LOCUS       DASYWL010000001         1672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_300261, whole genome shotgun sequence.
ACCESSION   DASYWL010000001 DASYWL010000000
VERSION     DASYWL010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300261"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1672
                     /locus_tag="VGR22_00005"
     CDS             <1..>1672
                     /locus_tag="VGR22_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEV2126985.1"
                     /translation="ATFWTNPGIEPAGIARAAVINVSGTGSSGASTITQQLVRSIYPE
                     KIGFEISYTRKFREALAAIALDQQYSKTDILTMYLNQIYYGVRSYGVEAAAQAFFQKK
                     ASELTLAEASLLAGLPQAPSFYDPSVPDNFPLAKRRQQYVLDQMVKDRYITQEEANAA
                     WNEPLDIHGNRTGAVKNAPHFTQYVREYIIEHYGIDALYGGLEITTSIDLDLQDQAEQ
                     IVADGVSNMEQYQRNNGAAVVMVPWSGEVLAMVGSADFNNALIAGQVNYTTSLIQPGS
                     SIKPLVYAAAFEQGWHPGTVILDVPTSWDSPDQPEPYEPQNYTRQFYGAMSARDALAN
                     SLNIPAVKATEYAGISGVMDLTRRMGYEDSLANDSSFYGLSLGLGSGEVELLEHTNAF
                     ATLANNGSHVPVHPILKIVDGQGNVLFELDDEEIAKEREQALPSGNAYQVTSILTDAE
                     AREMMFTSDNLFTRTQNELGRPVAAKSGTTEKWRDLWTMGYTTDVSVGVWVGRSGDTN
                     PDEMLSEIDGIQAAGPIWQDLMRLVHNDPQYAQLLDGPDGTSMPEEFPV"
BASE COUNT          333 a          546 c          527 g          266 t
ORIGIN      
        1 gcgaccttct ggaccaatcc cggcatcgag ccggccggca tcgcccgcgc cgcggtcatc
       61 aacgtgagtg gcaccgggtc atccggcgcg tccacgatca cgcagcagct ggttcgctcc
      121 atctaccccg agaagatcgg cttcgagatc agctacacgc gcaagtttcg tgaggcactc
      181 gcggccatcg cactggacca gcagtactcg aagaccgaca ttctcacgat gtacctgaac
      241 cagatctact acggcgtccg ctcctatggc gtcgaggcgg ccgcgcaggc cttcttccag
      301 aagaaggcgt cggagctcac gctcgccgag gcgtccctgc tggccggcct gccgcaggca
      361 ccgagcttct acgatccctc cgtgccggac aacttcccgc tcgccaagcg ccggcagcag
      421 tacgtgctcg accagatggt gaaggatcgt tacatcacgc aggaggaagc caacgccgcc
      481 tggaacgagc cgctggacat ccacggcaac cgcaccggcg ccgtcaagaa cgccccgcac
      541 ttcactcagt acgtccgcga gtacatcatc gaacactacg gcatcgatgc gctctacggc
      601 ggcctggaga tcaccacctc gatcgatctt gacctgcagg accaagctga gcagatcgtg
      661 gcggacggcg tcagcaacat ggagcaatac cagcgcaaca atggcgcggc ggtggtgatg
      721 gtgccgtgga gcggtgaggt gcttgcgatg gtgggctcgg ccgatttcaa caacgcgctc
      781 atcgccgggc aggtaaacta caccaccagc ctgatccagc cggggtcgtc gatcaagccg
      841 ctcgtctacg cggctgcctt cgagcaggga tggcaccccg gcacggtgat cctggatgtg
      901 ccgacgtcgt gggacagccc cgatcagccc gagccctacg agccgcagaa ctacaccagg
      961 cagttctatg gcgcaatgtc ggcgcgcgat gcgctggcga actcactcaa tatcccggcg
     1021 gtcaaggcga ccgagtatgc cggcatcagt ggcgtgatgg acctcacccg ccgcatgggc
     1081 tacgaggaca gcctcgcaaa cgactcctcg ttctacggtc tctcgcttgg cctcggttca
     1141 ggtgaggtcg agctgctgga gcacaccaac gccttcgcga cgctggcgaa caatggatcg
     1201 cacgtgccgg tgcatccgat cctgaagatc gtcgacggcc aagggaatgt gctcttcgag
     1261 ttggacgatg aggagatcgc gaaggagcgc gagcaagcgc tcccgtccgg caacgcttac
     1321 caggtaacct ccatcctcac cgacgcggag gcacgggaga tgatgttcac cagcgacaac
     1381 ctgttcacac ggacgcagaa tgagctcggc cgcccggtgg cggcgaagtc gggaacgacc
     1441 gagaagtggc gcgacctctg gacgatgggc tacacgacgg atgtctcggt tggcgtctgg
     1501 gtgggccgca gcggcgacac caaccccgac gagatgctct cggagatcga cggtatccag
     1561 gcggcaggtc cgatctggca ggacctgatg cggctggtgc acaacgaccc gcagtacgcg
     1621 cagcttctgg acggtccgga cgggacgtcg atgccggagg agttcccggt gc
//
LOCUS       DASYWL010000002        14224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_525534, whole genome shotgun sequence.
ACCESSION   DASYWL010000002 DASYWL010000000
VERSION     DASYWL010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 14224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14224
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525534"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="VGR22_00010"
     CDS             complement(<1..1015)
                     /locus_tag="VGR22_00010"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HEV2126986.1"
                     /translation="MAKRVKVVVTSDRYGNETTGLEMERKLISRYPELDIDLQGAPAR
                     SEDELIRIGQDADVLLVSTREEVTRHVLENIPNVKVISRYGVGLDNVDLDAAADVGIV
                     VTHYPGYCTSEVADHAMALLLAVNRRIVEQDRDLHEGAWQKYGSATGGILRGPVPPLR
                     QLTLGIIGFGRIGQALADRARPFGLKLLVADPYVSPGTVEATGAALLHLDEMLPQVDL
                     LTIHAPLTPETRGMINAERLSMMKPDASIINTARGPIIDLDALAEHLQEHPIARAALD
                     VVYPEPLPAAHPLYMLPNVIHTPHSAYYSEQSVITVREETFAEAMAVLMGKMPRTVAN
                     PAVL"
     gene            1204..2577
                     /locus_tag="VGR22_00015"
     CDS             1204..2577
                     /locus_tag="VGR22_00015"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF005914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEV2126987.1"
                     /translation="MSEQGWKQDLENRKDALERELMELLSMPSVSTDAQRVPDIRATA
                     EWVADRLRAAGVPEVRIAETEGHPSVLGRWHVSDDQPTVLIYGHYDVQPAEPLELWET
                     PPFDPAVRDGKVYARGSADMKGNLLTSLHGVEALARVNGQPPINVVFLYEGEEEIGSP
                     HLPKLVEQEKAFLACDAVLSADGSQFGPDQPELTVAMKGLGGCQINLTTANSDLHSGM
                     YGATVPNAVQAMVQLAATFHDVDGRVQVEGFYDKVHDLTEEERAEIAEVPFDEEEFRQ
                     ELGLDELWGEKGWTPRERMWARPTADFNGIWGGFQGEGTKTVTPREAHLKVTCRLVPD
                     QNPVEVVKLIEKHVQKHRPPGAKVEVEAIPGSAKPFVLDRSNPMNIAAGEVLEELYGK
                     KPYTTHAGGTIPATGIFQDVLGADTVGFAWSLPGSGAHAPNEWYRLEDYERGRVAYAM
                     LLERLKR"
     gene            2763..3641
                     /locus_tag="VGR22_00020"
     CDS             2763..3641
                     /locus_tag="VGR22_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2126988.1"
                     /translation="MDYVVFGIGFGATILVLGLLLRDLGPRLRYRRQANDDGVLPADV
                     LVAKVAWDRFCNALGAVLAIGGILFLLITFVCIILAVSDDTGGWVMLSALAVFFLMVL
                     YWTWAFFHRFGAYGILLERSAAEEEPEPEPAQRPEPMAGSEAGPVAVAPAADDAVEPQ
                     ERVIVGPVLDQVASRPEAEHEEVADEEAKIADSEPEAAEPENGEQTVVLTPEERLARH
                     EEPLDHGSAAGDLDETPGVARPSAARGRGTTDREITEDSAEPLEDETSEDEEQVDAAD
                     FDEDSEQDQEQGSDAR"
     gene            3732..3974
                     /locus_tag="VGR22_00025"
     CDS             3732..3974
                     /locus_tag="VGR22_00025"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV2126989.1"
                     /translation="MNFILWIILGIAAGWLAGVITRSNRGILGDLVLGLIGAFVAGWI
                     VDGSVGGDAGFWGSLVAATIAAVVLVLIKNAILGRD"
     gene            complement(4061..4699)
                     /locus_tag="VGR22_00030"
     CDS             complement(4061..4699)
                     /locus_tag="VGR22_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2126990.1"
                     /translation="MDLRYLRENSDEVWAKTLEHLSLSLTALAIALPLAIVLGILATR
                     IRWLTAPVVTTVSILYTVPSFAFMAFLIPGFGIGRQPALIMLVTYAQLFLVRNVVAGL
                     KSVDPAILEAARGLGMNELQLFWKVWLPLATPVIIAGIRTALLAIIAFATLTGYIRAG
                     GLGEIMFAGIGRNYPSQVLAGVIAVAGLAIVTDLLLRLLEQFTPVARARRAS"
     gene            complement(4721..5764)
                     /locus_tag="VGR22_00035"
     CDS             complement(4721..5764)
                     /locus_tag="VGR22_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2126991.1"
                     /translation="MTDVTAQDKPSGGSQIRLEHLTKIYPNTTAPAVDGVSLTVEPGE
                     FVVLLGPSGCGKTTLLKMVNRIYEPTSGQIWIDDTEIHALPASQLRRRIGYVIQQTGL
                     FPHMRIEDNVATVPKLLKWDRRRIRDRVRDLLELVGLPPDEYARRYPPQLSGGQQQRV
                     GLARALAGEPTTMLMDEPFGALDAITRTRLQDELKRIHRQISQTILFVTHDIDEAVRL
                     ADRIVVMREGHVVQFDTPLEIVTNPADAFVSDLVGAGDVLRRMGLITVASAASGATPF
                     QETEVEEHISGSAFLREAIGRMVESGTDRLTVLDEDGRPISELTMDDIRRAVGDEAAG
                     EAAEVDSIRSADG"
     gene            complement(5862..6860)
                     /locus_tag="VGR22_00040"
     CDS             complement(5862..6860)
                     /locus_tag="VGR22_00040"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV2126992.1"
                     /translation="MLNRRTVVKGALATPLAASALAAAQPAILAQDGPIVVGSKDFTE
                     QFILGNMYLMILEDMGLEAEDALNLGGTQIAHQALLSGEIQTYPEYTGTALVEVLGRS
                     VEEVLADFERGGGVATPAPGVASPVAGPASLEEYVYAIVRDAYQEQFDLTWLAFSPFN
                     NTQALAVRREFAEEHDLSTISDLAEIAGDLTISAPADFPEREDGLLGLQRVYNEGFDD
                     IDVLPVAPGLKYQALLDGEAEVVLAFGTDGQIAGYDLVVLEDDQGLWPPYNVAPIVKN
                     DVLEAHPEIEERFNAVTTSLTGEIMSELNWLVDGDERADPDQVAFEYLVENGFISE"
     gene            complement(6919..7560)
                     /locus_tag="VGR22_00045"
     CDS             complement(6919..7560)
                     /locus_tag="VGR22_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2126993.1"
                     /translation="MSTIDDVLGFYRDRSDQVLELTWQHIELSLVALALATLIFVPLG
                     IVFAHSERGGSGFVGAIASIRVIPSLALIFLFYPILGFGYEPALVALTVLAGPPLILN
                     TFAGMRQVDAAVLEAAKGVGMDPAQVFLKVHFPLALPVIVAGMRSASVEIFASATLAS
                     FIGVRTLGQYVITGISLLDTTYLLAGGMLIVALVLSAELVLGSIERLVRPPVA"
     gene            7873..8661
                     /locus_tag="VGR22_00050"
     CDS             7873..8661
                     /locus_tag="VGR22_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2126994.1"
                     /translation="MLGRFRSRTSKQPLTDGDKIIAAQDVVKIYETGATRVEALKEVS
                     FSVDRGEMVAVMGPSGCGKTTLLNCLSGLDSVTSGEVWLDGQDLAEMSDKKRTDYRAR
                     RMGFIFQVYNLLPVLSAVENVELPLLVAGVSPKEARRRAMTALEQVGLRDWADHRPAE
                     LSGGQRQRVTVARSLVNDPAIVWADEPTGALDSKSAQDIMDLLRNLNEEQDLTIVLVT
                     HDSDVGAQAHRIVRMQDGEIVGEERPERGDYVHAPADAEVVATR"
     gene            8715..11732
                     /locus_tag="VGR22_00055"
     CDS             8715..11732
                     /locus_tag="VGR22_00055"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV2126995.1"
                     /translation="MVTIMVVMVALLLVCLAVIAYITWRRPVIFKLGVRNIPRRKAQT
                     VLIVVGLMLSTLIIAAALGTGDTLNRSVSTVVYELLCPVDELVVASADGDGEGELSAV
                     ITQTIPESSIETVREIVDDSDAVDAVGGLLIAFAPALNVGDTDPDELDSFNDVVDTAA
                     RSEPGVVLAGIDPVSFEDLGGTVDLEGDRVDFATFGDRDVLLSESGAEDLDAEVGDTL
                     VASVNNTPVTLQVAAIAEDSVLTGSLDSTGAPAMAMRLDVLQELTGREGRLSGIGISN
                     VGGPREGLERTDEVVQLLKPELAALGLGINTIKQDLVDQAEQIANIFVTFFVVFGLFS
                     IAVGILLIVLIFTMLAAERRSEMGMERAVGAQRRMLIQQFLSEGAGYALLAGLVGTML
                     GALAAVGIGLGIQAAFGEAIDISPYIEPRSMVIAYSPGVVITFLAIAVSSWRVSRLNV
                     VAAVRDIPDTYQPRKNRRQLVWGVLMVVVGVLLIVAAQPDRSLFIFTAGMTLIPFGIA
                     AIVTYFGWHPRWVLTAAGLCTLVFWLLPEDALGSIFGEYSGNIEMFFVSGICIVAAST
                     LVIIQNFGWTVGGVSRLGGQAAGWVPAIRLATSYPSANKGRTGMMIAMFSLIVFSLVV
                     VASINENFAAAFLSEEATAGWDVEVETTTTNPVPDLEARLEEVGYDTSQVAAIGTITS
                     PAEAASTTLRNPDDEEWKTSLIQVAGQDYLEHATLEFSARAEGYEDDEAIVEALMTEP
                     DVMVIDTFSLATQQEFGGNPDMFTLDEIEASGTFDAPVVEVRLPDGTVEELRVIGVIS
                     TSVSSLFGTFVGPEADDALFSDVGEPERTWYVELVPGVDPSTAAAEVERALLASGVQA
                     YDVRQEMEDAQQQQRSFLYIMQGFMGVGLIVGIAAVGVIAFRAVVERRQQIGMLRALG
                     FQRGVVARAFVIESAVIVIIGVLAGGIMGLILSWSFITGDEFSAAGNATFIVPWQLIS
                     ITLIAAIVAALLMTWIPARQASRVVPAEALRYE"
     gene            11881..13329
                     /gene="pyk"
                     /locus_tag="VGR22_00060"
     CDS             11881..13329
                     /gene="pyk"
                     /locus_tag="VGR22_00060"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEV2126996.1"
                     /translation="MERRVKIVSTLGPSVSTPERLYQLLEAGVDVVRINAAHGTNETR
                     AELIRYVRKAADAIGKLVPILFDLQGLKIRTGPLPEGSDPLPIARGTSVRLVPRPEPT
                     SPDQIGVTLGTLLDVISPGSRVLISDGLIELRVTEVTSDHAVATVGRGGLLLGRQGVT
                     LPGAPIRGGALTDADKADVAFAMGQKVDYFGLSFINDASDLELARNEALKHGPTVPGL
                     IAKIERPEALDNLRDIACEADGLMVARGDLGVQLPPEQVPRAQKHIIGIANEIGVPVI
                     TATQMLESMITQPVATRAETSDVANAVWDGTDAVMLSAETAVGKYPIEAVLTMDRIIK
                     EVEKDGPARSQHASVHAIIRPDDEAQRFADAIARAAYALAVDTPVRFLVVFTKTGSAV
                     RRIAKYRPSPPIIAVADNEETARRLGLVWGVRSIVVPVETDPDAVFRKAGQQIVAEGL
                     AKHGEYALLVGSLPMTQEAGRTNLVHFRQLGT"
     gene            complement(13483..>14224)
                     /locus_tag="VGR22_00065"
     CDS             complement(13483..>14224)
                     /locus_tag="VGR22_00065"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEV2126997.1"
                     /translation="TLMCISNRIGAGLFSNARWQGVRLRDVLMAAGVDEDAYELKLTG
                     ADAYVDTFGMDYALNDTTLIVYGMNGEPLPRTHGYPVRIIVPGLFGEKNIKWVTRIDV
                     VTEDHQGFYEQQGWGPSFVPPTRSDVFRPRTAGAGQRATFRQPIAAGSTVDLRGRAFA
                     GDRGIRSVEVTTDDGATWSPAEIYYPGTRLTWSLWRFNWTPGAPGEYRVYSRAVDGNG
                     DPQPSEPRGIIPQGAQGYNILTATVVEA"
BASE COUNT         2456 a         4413 c         4868 g         2487 t
ORIGIN      
        1 tcagcacggc cgggttggcg accgtgcgcg gcatcttgcc catcagcacc gccattgcct
       61 ccgcgaacgt ctcctcgcgt acagtgatga ccgactgttc ggaatagtag gccgagtgcg
      121 gcgtgtggat cacgtttggc agcatgtaga gcgggtgggc ggcgggaagc ggttccgggt
      181 agaccacatc gagcgcggct cgtgcgatcg ggtgttcctg caggtgctcg gcgagggcat
      241 cgaggtcaat gatcggtccg cgggcggtgt tgatgatcga ggcgtccggc ttcatcatcg
      301 acagccgctc agcattgatc atgccacgtg tctccggtgt gagcggcgcg tggatggtca
      361 gcaggtcgac ctgcggcagc atctcgtcca aatgcagcag ggccgcgccg gtggcctcaa
      421 cggtgcccgg cgagacatac gggtcggcga cgagcagctt caggccgaac ggacgcgcgc
      481 gatcggcgag cgcctgaccg atgcgaccaa agccgatgat gccgagcgtc agctggcgca
      541 gcggcggcac ggggccgcga agaatgccgc ccgtcgcaga gccgtacttc tgccacgcgc
      601 cctcgtggag atcgcggtcc tgctccacga tgcgccggtt cacggccagc agcagcgcca
      661 tggcatggtc ggcgacctcc gacgtgcagt agccggggta gtgggtgacg acgattccga
      721 cgtcggcggc cgcgtcgaga tcgacgttgt ccaagccgac gccgtagcgg ctgatgacct
      781 tgacgttggg aatgttctcg agcacatggc gggtaacctc ctcgcgcgtg ctcacgagca
      841 gcacgtcggc gtcctgcccg atgcggatca gctcgtcctc ggagcgcgcg ggcgcgccct
      901 gaaggtcgat gtcgagctcc ggatatcggc tgatgagctt gcgctccatc tcgagacccg
      961 tggtctcgtt gccgtaccgg tccgatgtca cgacaacctt gactcgtttc gccacgctct
     1021 tgctcctgct gagaaatcgg gcggttcttt cgccccggtg agtgaaccag ggccattctg
     1081 gcacaccgcg tcgtgcctgc aaacgggctt gatggtgacg gaccgggtcc attccgcgat
     1141 actttcccgc gaaccgaaca gacggcgggc catgatgccc cagagacggg agaacgagac
     1201 gacatgtcgg agcaaggctg gaagcaggac ctcgaaaacc gcaaggacgc gctcgagagg
     1261 gagttgatgg agcttctctc gatgccgagc gtcagcaccg acgcgcagcg ggtgccggac
     1321 attcgcgcga cggccgagtg ggtcgccgat cggctccgcg cggcaggggt gccggaggtg
     1381 cgaatcgccg agacagaggg gcatccgtcg gtgctcggac gctggcacgt gtctgatgac
     1441 cagccgacgg tgctgatcta cgggcactac gacgtgcagc cggccgagcc gctggaactg
     1501 tgggaaacac cgccgttcga cccagcggtg cgggatggca aggtctacgc gcggggctcg
     1561 gccgacatga agggtaacct gctcacgtcg ctgcacggcg tcgaggcgct ggcgcgggtg
     1621 aatggccagc cgccgatcaa cgtcgtcttt ctctacgagg gtgaggagga gatcggcagc
     1681 ccgcacctgc cgaagctggt cgagcaggag aaggcgtttc ttgcctgcga tgccgtgctc
     1741 tccgccgatg gaagccagtt cggcccggac cagccggagc tgacggtcgc gatgaagggg
     1801 ctcggcggct gtcagatcaa cctcaccacc gccaacagcg atcttcactc gggcatgtac
     1861 ggcgccaccg tcccaaacgc ggtacaggct atggtgcagc tggcggcgac attccacgac
     1921 gtcgacggcc gcgttcaggt ggaagggttc tacgacaagg tgcacgacct cactgaggag
     1981 gagcgcgccg agatcgccga ggtgccgttc gacgaggagg agttcaggca ggaactcgga
     2041 ctcgacgagc tctggggcga gaagggctgg acgccgcgtg agcggatgtg ggcacgtccg
     2101 acggccgatt tcaacggcat ttggggggga ttccagggcg aaggcaccaa gaccgtgacg
     2161 ccgagggagg cgcacctcaa ggtcacctgc cggctcgtgc cggatcagaa ccccgtcgag
     2221 gtcgtgaagc tgatcgagaa gcacgtccag aagcaccgcc cgcccggcgc aaaggtggaa
     2281 gtcgaggcga tccccggttc cgccaagccg tttgtgctcg atcggtccaa cccgatgaac
     2341 atcgccgcag gcgaggtgct ggaggagctc tacggcaaga agccgtacac cacccacgcg
     2401 gggggcacga tcccggcaac cggcatcttc caggacgtgc tcggtgcgga caccgtcggc
     2461 ttcgcgtgga gccttccagg cagtggcgcg cacgccccga acgagtggta ccgcctcgag
     2521 gattacgagc gggggcgcgt cgcctatgcc atgctgctgg agcgcctgaa gcggtagctg
     2581 acggacacga gttgcgataa cgagcggccc gcctgcgcgg cgagtcgctc cattcttgtg
     2641 ccagtcgagg ctggtagtat tgacggcagc gtggctcgcg cgtgtgacgg ggaccgatcg
     2701 agagcgggta gccgacgaga agttatcggc cggcttgcgt gcgatgggac aaggggagtc
     2761 ggttggatta cgtcgtattc gggatcgggt tcggggcgac catcctcgtg ctaggtctgc
     2821 tgctccgcga cctcggtccg cgccttcgat accgacgcca ggcaaacgac gatggtgtcc
     2881 ttcccgcaga cgtcctggtc gccaaggtcg cgtgggaccg cttctgcaac gccctcggag
     2941 ccgtgctggc gatcggtggc atcctcttcc tcctgattac cttcgtctgc atcatcctgg
     3001 cggtctcgga tgacacgggc ggctgggtca tgctctcggc gctcgcggtg ttcttcctga
     3061 tggtgctcta ctggacgtgg gcattcttcc accgattcgg cgcctacgga atcctgttgg
     3121 agcggtcggc tgccgaggaa gaacccgagc cggaacctgc acagaggcca gagccgatgg
     3181 cgggatcgga agctggcccg gttgcagtgg cgccggcggc cgatgatgcc gtggagccgc
     3241 aagagcgagt gatcgtcggc ccggtacttg accaggttgc gtcccgaccc gaggcggagc
     3301 acgaagaggt cgctgatgag gaggcaaaga tcgccgattc cgagccggag gctgccgaac
     3361 cggaaaacgg cgaacagacg gtggtgttga ctcccgagga gcgcctggcg cggcacgagg
     3421 agccgctcga ccacggctcc gctgccggcg atctcgacga gaccccggga gtggcgcgcc
     3481 cgtcggctgc tcgtggtcgg ggtacgaccg atagggagat tacagaggat tccgccgagc
     3541 cacttgagga tgagacgtcc gaggacgagg agcaggttga cgcggctgac ttcgatgaag
     3601 attcggagca ggatcaagag cagggctccg acgcgcgctg atccgtggct ggcacaggga
     3661 atgcgtacag gtgggcgcta tcgtaggggg cggtgggcct ccgaatatcc ggagtgggag
     3721 gggcaagagc aatgaatttc atcctgtgga tcatattagg gatcgcggcg ggctggcttg
     3781 ctggcgtgat cacccgcagc aaccgaggaa tcctcggcga tctggtgctc ggcctgatcg
     3841 gcgcctttgt ggccggctgg atcgtcgacg gatcggtcgg cggtgacgcc gggttctggg
     3901 gcagcttggt cgcggcgacg atcgccgccg tggtgctggt gctgatcaag aacgcgatcc
     3961 tcggccgcga ctaggcatcc gctgatgcac gcgtcgccgc gcccatgcct gtgtcccgag
     4021 cctctgtaaa ggcgatcctg gctgggcgtg tccgatttca ctatgaggcg cggcgggctc
     4081 gggcgaccgg ggtgaattgc tcgagcaggc gcagcagcag atcggtgacg attgccagcc
     4141 ctgcgaccgc gatcacgcca gcgagcacct gcgaggggta gttgcggccg ataccggcaa
     4201 acatgatctc gccgagcccg cccgcgcgga tgtagccggt cagcgtcgcg aaggcgatga
     4261 tcgccagcag ggctgtgcgg atgccggcga tgatcaccgg cgtcgccagc ggcaaccaga
     4321 ccttccagaa cagctgtaac tcgttcattc cgagtccgcg cgccgcctcg aggatcgcgg
     4381 ggtcgacgct cttgaggccg gcgacgacgt tgcggaccag aaacagctgc gcgtaggtga
     4441 ccagcatgat caacgccggc tggcgcccga taccgaatcc tggtatcagg aacgccatga
     4501 acgcgaatga cggcaccgtg tagaggatgc tgactgtggt caccaccggt gccgtcagcc
     4561 accgaatgcg cgtcgcgagg atgccgagta cgatcgccag aggcagcgcg attgccaggg
     4621 cagtgagcga gagcgacagg tgctccagcg tctttgccca gacttcgtcg ctgttctcac
     4681 gcagatagcg aagatccatg ccaatcttcc cggctggtca tcagccgtcg gctgagcgga
     4741 tggaatcgac ctccgccgct tcgccggcag cctcatcacc aaccgcccgg cggatgtcgt
     4801 ccatcgtgag ctcggaaatc ggccgaccgt cctcgtccag aaccgtcaga cggtcggtac
     4861 cggattcgac catccgcccg atcgcctccc gcaggaacgc cgacccggaa atgtgctcct
     4921 cgacttctgt ttcctgaaat ggcgtggctc ccgatgcggc cgatgcgacg gtgatgagtc
     4981 ccatgcggcg cagcacatcg ccggcgccga cgagatcgga tacgaacgcg tcggccgggt
     5041 tggtgacgat ctccagtggc gtgtcgaact gcacgacatg cccctcccgc atcaccacga
     5101 tgcggtccgc gagtcgcacg gcctcgtcga tgtcatgggt gacgaagaga atcgtctggc
     5161 tgatctgccg gtggatgcgc ttcagctcgt cctgcaagcg tgtccgcgtg atggcgtcca
     5221 acgcaccgaa gggctcgtcc atcagcatcg tggttggttc gccggcgagg gcccgggcga
     5281 gcccgacgcg ctgctgctga ccgccggaaa gctggggcgg gtagcggcgg gcgtactcat
     5341 ccggaggcag gccgacgagc tccagcaggt cgcgcacgcg gtctcggatg cgtcttcggt
     5401 cccacttcag gagtttgggt acggtggcga cgttgtcctc gatccgcatg tggggaaaga
     5461 ggccggtctg ctgaatgacg tagccgatcc tccgccgcag ctgcgaggcc ggcagcgcgt
     5521 ggatctccgt gtcgtcgatc caaatctggc cgctggtggg ctcgtagatg cggttgacca
     5581 tcttgaggag cgtggtcttc ccgcatccgg acgggccgag gagcaccacg aactcgcccg
     5641 gctcgacggt gagcgagacg ccatcgacag caggtgcagt ggtgtttggg tagatcttgg
     5701 tgagatgctc gagccggatt tggctgccgc cgctgggttt gtcctgggct gtcacgtcag
     5761 tcacctcagc tctctcgcgt cgctcggcgg aatgcacacg gcagcataca caagaatggg
     5821 tctgccgata tgtagcggca gacccgggcc tcaaaacggc gttactcgct gatgaatccg
     5881 ttctcgacga ggtactcgaa ggccacttgg tccgggtcgg cccgctcgtc gccgtccacg
     5941 agccagttga gctcggacat gatctcgccg gtgagcgagg tcgtgacggc gttgaagcgc
     6001 tcctcgatct ccggatgggc ctccaggaca tcgttcttca cgatcggggc gacgttgtag
     6061 ggtggccaca ggccctgatc gtcctccagc acgacgaggt cgtagccggc gatttggccg
     6121 tcggtgccga aggcaagcac gacctcggct tcgccgtcga gcagtgcctg gtacttcagc
     6181 cccggcgcca ccgggagcac atcgatgtcg tcgaatccct cgttgtagac gcgctgcagg
     6241 ccgagaagcc cgtcctcgcg ctcggggaaa tcggctggcg cggagatcgt gaggtcgccg
     6301 gcgatctcgg ccaggtccga gatggtgctg agatcgtgtt cctcggcgaa ctcgcgtctc
     6361 acggcgaggg cctgagtgtt gttgaacggg gagaaagcga gccaggtgag gtcaaactgt
     6421 tcctggtagg cgtcgcggac gatggcgtag acgtattcct ccagcgatgc tggtccggca
     6481 acggggctgg caacgccggg cgccggcgtg gcgacgccgc caccgcgctc gaagtcggcc
     6541 agaacctcct cgacggaccg gccaagcacc tcgaccagcg ccgtgcccgt gtattcgggg
     6601 taggtctgga tttcgccgct caacagcgcc tggtgggcga tctgcgtgcc gccgaggttg
     6661 agggcatcct cggcttcgag acccatgtcc tcgaggatca tgagatacat gttgccgagg
     6721 atgaactgct cggtgaagtc cttggacccc acgacaatgg gtccgtcctg cgcgaggatg
     6781 gccggctggg cggctgcgag cgcggaagcg gcgaggggcg ttgcgagagc gcccttgact
     6841 acggtgcgtc gattcagcat ggtgtgtgtc attcctttcc gtcgaacgat tcaatgcctg
     6901 tggctttcac gagcccgatc aggcgacagg tggccgcacg agccgctcga tactgccgag
     6961 gaccagctcc gccgagagca cgagtgccac gatcagcatg ccgccggcca gcaggtaggt
     7021 ggtatccagc aaggatatcc ccgtgatgac gtactgaccc agcgtgcgca cgccgatgaa
     7081 cgaggcaagt gtcgcacttg cgaagatctc cacggatgca gaccgcatgc ccgctacgat
     7141 gacgggtaac gcgagcggaa aatgcacctt caggaagacc tgcgcagggt ccatgccaac
     7201 ccccttggcc gcctcgagga ccgcggcatc cacctgccgc atgccggcga aggtgttcag
     7261 gatcagcgga ggccctgcca gtacggtgag cgccacgagc gccggctcat acccgaaccc
     7321 gaggatcggg tagaagagga agatgagcgc cagcgaaggg atcacgcgga tcgaggcgat
     7381 cgcgccgacg aagcccgatc caccgcgctc ggagtgggcg aagacgatgc ccagcggcac
     7441 gaagatcagc gtcgccagcg cgagtgcgac cagactgagc tcgatgtgct gccacgtcag
     7501 ctcgagaacc tggtcggatc ggtcccggta gaagccgagg acatcgtcga tggtactcac
     7561 ccgcggcggt ccctcctcgg cggcgcgcag cttccagctc cgcacaccct gctctagcca
     7621 actgccgtcc agcggctgtg accagcatac cgcggctcgt catccgtgcc atgagcgagt
     7681 tgcggcgatg gttcttgtcg gccactacga gccagtctgg acgacgcgat tcggcctccc
     7741 gtaacatctt tgccgcatgc gcagcgtaca ctggagacag cacgcagctc ttgccgcacg
     7801 tgctgggtcc cacctgcaac ggtccagaat ctcgatcccg ccaacccggc ggatttactg
     7861 aggagtcatc ttatgctggg acgctttcgc tccagaacat ccaagcagcc actgacggat
     7921 ggcgacaaga tcatcgccgc gcaagacgtc gtcaagatct acgagaccgg tgccactcgc
     7981 gtcgaagcgt tgaaggaagt gtcgttctct gtcgatcgcg gcgagatggt ggccgtgatg
     8041 ggtccctccg gctgcggcaa gacgacgctg ctcaactgcc tctcagggct ggactccgtc
     8101 acctctggtg aggtctggct cgacggccaa gacctcgccg agatgagcga caagaagcga
     8161 acggattatc gtgcccgccg catggggttc atctttcagg tatacaacct tctgcccgtc
     8221 ctgagcgcgg tggagaatgt cgagctgccg ctgctggttg cgggcgtcag cccgaaggag
     8281 gcgcggcggc gggcaatgac ggccctggag caagttggcc tgcgcgactg ggctgaccac
     8341 aggccagccg agctctccgg tggtcagcgc cagcgggtga ccgtggcacg atcgctggtc
     8401 aatgatccgg ccatcgtctg ggccgacgag ccgaccggcg cgctcgattc gaaatccgca
     8461 caagacatca tggacctgct gcggaacctt aatgaggagc aggacctgac catcgtgctc
     8521 gtcacacacg attccgacgt gggcgcgcag gcccatcgca tcgtccgcat gcaggacggt
     8581 gagatcgtcg gcgaggagcg ccctgagcgg ggtgactatg tccatgcgcc ggccgacgcc
     8641 gaggtcgtcg caacccgcta gacgtctcgc ctcggattca ggagtaactc acggaagcgc
     8701 tctttggcat ccccatggtc acgatcatgg tggtgatggt tgcgctgctg cttgtctgcc
     8761 tggcagtcat cgcatacatc acctggcgtc gccctgtcat cttcaagctg ggtgtcagga
     8821 acataccccg gcgcaaggcg caaactgttc tgatcgttgt cggcctcatg ctctcgacgc
     8881 tgatcatcgc ggcggcgctg ggcaccggtg acaccctgaa caggtccgtg agcacggtgg
     8941 tgtacgagct gctgtgcccg gtcgacgagc tcgtggtggc gtcggccgac ggtgacggcg
     9001 agggcgagct cagcgcggtg atcacgcaga cgatcccgga atcgtcgatc gagaccgtgc
     9061 gagagatcgt ggacgacagt gatgcggtcg acgcggttgg cgggctcctg atcgcgtttg
     9121 ccccggcgct caatgttgga gacacggacc ccgacgaact tgactcattc aatgacgtcg
     9181 tcgacactgc ggcgcggtcc gagccgggcg tggtcctggc ggggatcgat ccagtatcgt
     9241 ttgaggatct cggcgggacg gttgacctcg aaggtgaccg tgtcgatttc gccacgttcg
     9301 gtgaccgcga cgtgctcctg agcgagagcg gcgccgagga tctcgacgcc gaggtgggag
     9361 ataccctcgt cgcttcagtc aacaacacac cggtcacgct tcaggttgcc gcgattgcag
     9421 aggactctgt cctcaccggc agcctcgaca gcacgggtgc gcccgcaatg gccatgcggc
     9481 tcgacgtcct tcaggaactg acgggcaggg aaggacggct gagcggcatc gggatctcga
     9541 acgttggcgg tcctcgggaa ggtctggaga ggaccgacga ggtggtccag ctgctcaagc
     9601 ccgagcttgc agctctgggg ctcggcatca acaccatcaa gcaggacctg gtggaccagg
     9661 ccgagcagat cgcaaacatc ttcgtgacgt tcttcgtggt gttcgggctc ttctcgatcg
     9721 ccgtcggcat tctgctgatt gtgctgatct tcaccatgct cgcggccgag cgacggtcgg
     9781 agatgggcat ggagcgggcc gtcggcgccc aacggcggat gcttatccag cagtttctct
     9841 ccgagggcgc agggtacgcg ctgctggccg ggctggttgg cacgatgctc ggggcgctgg
     9901 cggccgtcgg gatcggcctt ggcatccagg cagcgtttgg cgaggctatc gatatcagtc
     9961 cgtatatcga gccgcgaagc atggtgatcg cgtactcgcc cggagtggtg atcaccttcc
    10021 tggcgatcgc cgtttcgtcg tggcgagtga gccggctgaa tgttgtcgcg gccgtccgcg
    10081 atatcccgga tacctatcag ccgaggaaga atcgccggca gctcgtctgg ggcgtgctca
    10141 tggtggtggt cggagtcctg ctaatcgtcg ctgcgcagcc cgaccgcagc ctgttcatat
    10201 tcaccgccgg catgacgctg atcccgttcg gcattgccgc gatcgtgacg tacttcggtt
    10261 ggcacccgcg ctgggtgctt actgcggccg ggctctgcac gctcgtcttc tggttgctgc
    10321 ctgaggatgc cctcgggtcg atcttcggcg agtacagcgg caacatcgag atgttcttcg
    10381 tctccggcat ctgcatcgtg gcagcctcga ccctggtgat catccagaac ttcggctgga
    10441 cggtcggggg ggtcagccga ctcggcggtc aggctgccgg ctgggtgccc gcgattcggc
    10501 tggcgacctc ctacccgagc gcgaacaagg gtcgcaccgg catgatgatc gccatgttca
    10561 gcctgatcgt gttctcgctt gtggttgtgg cgtcgatcaa cgagaacttc gctgccgcct
    10621 tcctcagcga ggaagcaacc gctggctggg acgttgaggt cgagacaacc acgaccaacc
    10681 cggttcctga tctcgaagcc agactggagg aagtcggcta cgacacgtca caagttgccg
    10741 ccatcgggac catcacatcg ccggcggagg cggcgtcaac cacgctccgg aatcccgacg
    10801 acgaagagtg gaagaccagc ctgattcagg tggccgggca ggattacctg gaacatgcca
    10861 cactggagtt cagcgcccgg gcggagggct atgaggacga cgaggcgatc gtcgaagcgc
    10921 tcatgaccga gccagacgtg atggtgatcg acaccttctc cctagcgacg cagcaggagt
    10981 tcggcggcaa tcctgacatg ttcacactcg atgagatcga ggcgagcggc accttcgacg
    11041 cacccgtcgt ggaggtgcgg ctccccgatg gcacagtgga ggagttgcgc gtcatcggtg
    11101 tcatcagcac gagtgtctcg agcctcttcg gcaccttcgt cgggccggaa gccgatgacg
    11161 ccctcttctc cgatgttgga gagccggagc gaacgtggta cgtggagttg gtgccgggtg
    11221 ttgaccctag caccgctgcc gccgaggtcg agcgcgcgct gctggcgtcc ggcgtacagg
    11281 cgtatgacgt tcgacaggag atggaggacg cccagcagca gcagcggtcg ttcctctaca
    11341 tcatgcaagg attcatggga gtcggactga tcgtcgggat tgccgcagtg ggcgtgatcg
    11401 cctttcgcgc cgtggtggag cggcgtcagc agatcggcat gctgcgagcc ctcggcttcc
    11461 agcgcggggt ggtcgcgcgg gccttcgtga tcgaatctgc cgtcatcgtg atcatcggcg
    11521 tgctggccgg tggcatcatg gggctgatcc tgtcctggtc gttcatcacc ggtgatgagt
    11581 tctcggcggc cgggaatgca accttcatcg tgccgtggca gctgatcagc atcaccctga
    11641 ttgcggcgat cgttgcggca ctgctcatga cgtggatccc tgcccggcag gcctcccggg
    11701 tagtgcccgc cgaagcgctc cggtacgagt agcctctccc ggcccatgtc gccaatggga
    11761 cgcggcggac ggctgcgagt gctggacgag cgaatgcgcc atacctgcta gtatggcgag
    11821 cggaacgcgt cgtggccgag cggtcgcgac ccgagtatca gaaaacgagg tcgcccctac
    11881 atggaacgac gcgtcaagat cgtctcgacg cttggcccat ccgtgagcac gcccgagcgg
    11941 ctctatcagc tgctggaggc gggcgtcgat gtggtgcgta tcaatgcggc acacggcacc
    12001 aatgagacgc gcgctgagct gatccgatac gtgcgcaagg cggcggacgc gatcggcaag
    12061 ctcgtgccga tcctcttcga cctgcagggc ctcaagatcc gcactggacc gctgccggag
    12121 ggctctgacc cactaccgat cgcgcgcggc acctcggtgc ggctcgtgcc gcgaccggag
    12181 ccgaccagcc ccgaccagat cggcgtgacg ctgggcacac tgctcgatgt catctcgccg
    12241 gggtcgcgcg tgctgatctc cgacggcctg atcgaactcc gtgttaccga ggtcacgagc
    12301 gaccatgccg tggccaccgt cggccgtgga gggttgctgc tgggcaggca gggcgtcacc
    12361 ctgccgggcg cgccgattcg cggcggggcg ctcacggatg ctgacaaggc ggacgtcgcc
    12421 ttcgccatgg ggcagaaggt cgactacttc gggctgagct tcatcaacga cgcaagcgat
    12481 cttgagctgg cccgcaacga ggcgctgaag cacggcccca ctgtcccggg gctgatcgcc
    12541 aagatcgagc ggccagaggc gctagacaat ctccgcgata tcgcctgcga ggcggatggc
    12601 ctgatggtcg cacgtgggga tctcggcgta cagctgccgc cggagcaggt gccgcgggcc
    12661 cagaagcata tcatcggcat cgccaatgag attggcgtac ctgtcatcac ggcaacccag
    12721 atgctggagt cgatgattac ccaaccggtc gcgacccgcg ccgagacgag cgatgtcgcc
    12781 aacgccgtct gggacggcac cgacgcagtc atgctctcgg cagagacggc tgtcggcaag
    12841 tacccgattg aggccgtgct gacgatggac cggatcatca aggaggtcga aaaggacggg
    12901 ccggctcgtt cgcagcacgc gtctgtccac gcgatcatcc gccccgatga tgaggcacag
    12961 cgattcgccg acgcgatcgc gcgggcggcc tatgcgctgg cagtcgacac gcccgtgcgg
    13021 ttcctggtgg tgttcaccaa gacaggctcg gccgtgcggc gcatcgccaa gtatcggccg
    13081 agtccgccga tcatcgctgt ggccgataac gaggagactg cacggcggct cgggctcgtc
    13141 tggggcgtgc gcagcattgt cgtgccggtg gaaaccgatc ccgatgccgt cttccgcaag
    13201 gcgggccagc aaattgtcgc cgagggactc gcaaagcacg gcgagtacgc gttactggtg
    13261 ggatcgctgc ccatgaccca ggaggccgga cgcaccaatc tggtgcattt ccggcagctt
    13321 ggtacgtagt tgcgcaccgc aacatcgatc ggttgtggag acccgcctgc agagaggcgg
    13381 gtctcgtcgt ttctgcgtgg gcgcgaaaac cgacgtgcag gcgtggtcgg attttcctcg
    13441 agagggccaa tgccggactg ccggattacc cgctgtggat cctcacgcct cgacgacggt
    13501 ggcggtgagg atgttgtatc cctgcgctcc ctgcgggatg atgccccgcg gctccgacgg
    13561 ctgcgggtca ccgttgccgt ccactgcccg cgagtacacc cggtactccc ccggcgcccc
    13621 cggtgtccaa ttgaaccgcc agagggacca agtgagccgc gtgccggggt agtagatctc
    13681 cgccggcgac caggtcgcgc cgtcgtcggt cgtcacctcg accgaccgga tcccgcggtc
    13741 gcccgcgaat gcccgtcccc ggagatcgac cgtactgccg gcggcgatcg gctggcggaa
    13801 ggttgcccgc tgcccggcgc cggctgtccg tggccggaag acgtccgagc gggtcggcgg
    13861 cacgaagctc gggccccagc cctgctgctc gtagaagccc tggtggtcct cggtgacgac
    13921 gtcgatgcgc gtcacccact tgatgttctt ctcgccgaag agccccggca cgatgatccg
    13981 gaccggatag ccgtgggtgc gcggcagcgg ctcgccattc atcccgtaaa cgatcagcgt
    14041 cgtgtcgttg agggcgtaat ccatcccgaa ggtatcgacg taggcgtcgg ccccggtcag
    14101 cttgagctcg taggcatcct cgtcgacgcc cgccgccatc agcacgtcgc gcaggcgcac
    14161 gccttgccac cgggcattgc tgaagagccc tgcgccgatg cggttgctga tgcacatcag
    14221 cgtc
//
LOCUS       DASYWL010000003         2175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_544327, whole genome shotgun sequence.
ACCESSION   DASYWL010000003 DASYWL010000000
VERSION     DASYWL010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544327"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..153
                     /locus_tag="VGR22_00070"
     tRNA            64..153
                     /locus_tag="VGR22_00070"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:98..100,aa:Ser,seq:cga)
     gene            179..270
                     /locus_tag="VGR22_00075"
     tRNA            179..270
                     /locus_tag="VGR22_00075"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:215..217,aa:Ser,seq:gga)
     gene            complement(338..1261)
                     /locus_tag="VGR22_00080"
     CDS             complement(338..1261)
                     /locus_tag="VGR22_00080"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEV2126998.1"
                     /translation="MEGDSDKADIVVAGAINTDLVAVVERAPQAGETVTGSSFSIFSG
                     GKGANQAIAAQRSGSLTAMVGAVGDDAFGMDRRADLAADGIIVDGITEFAGMASGVAL
                     ITVEREGENRIAYVPGPTLRVTGEQIRGALEAHRPAIYLQPNEVPSEVAAVAIRTARE
                     LGASTVLNAAPDPTTVWPVLPDVQYLIVNEVEAFALVDESGPLDEIAIDLARRSGTNV
                     VLTAGAVGAYGVEDGEVFHVAAPAVQVVDTTGAGDTFCGAFAACLAHGVPFGEAVAWS
                     VVAGSLATTISGAQPSIPRREVVEGFITDSR"
     gene            complement(1286..>2175)
                     /locus_tag="VGR22_00085"
     CDS             complement(1286..>2175)
                     /locus_tag="VGR22_00085"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEV2126999.1"
                     /translation="TSGIDNPYDRAKAIELFLRNHIVYDLEVGVAPEDRDIVDYVVFE
                     NRRGYCEHYATSMTVMLRTLGIPAKTVVGYYPGDWDEEANGYIYRQQQAHAWTEAFFP
                     EYGWIRFEPTASQPESTLDGAAAADVSDEIPTPTPELETVAPEPPVATPDLGDDSAAP
                     IADVVPETPDEDEDGPGWPLPLGAGVLAVAALASGVWWVATRSPALDPGALFGAMVRW
                     GRFAGIRGNPSETPREYARQLGYRYPDLAGDAHDIVDVYEDHQYGGRAPERTRLERAG
                     DAIRQMQRALIRRFVRFGR"
BASE COUNT          366 a          760 c          678 g          371 t
ORIGIN      
        1 tttccgttgc acggcgacac cgcgcggctg acagcgatca ccggttttgg tagactatcc
       61 ggcggagagg tgtccgagtg gtttaaggtg ctgctctcga aaagcagtgt ggtgtaacag
      121 ccaccgtggg ttcgaatccc accctctccg ccacgggccg agggtcggtc acagccgagg
      181 aagggtcgca tagtctggcc tagtgcgcgc gattggaaat cgcgtaggcg gtaacacgcc
      241 tcgagggttc gaatccctcc ccttccgcca cacagaaccg cccggctaga agcgagccgg
      301 gcggttccgt tactcggatc aacggctggg ccggctgtca cctgctgtcg gtgatgaacc
      361 cttcgactac ctcccgtctc gggatcgacg gctgcgcccc ggagatcgtt gtcgccagcg
      421 atccggcaac gaccgaccac gccactgcct ccccgaacgg cacaccatgc gccagacatg
      481 cggcaaacgc gccgcagaac gtgtcgcccg cgcccgtggt gtccacgacc tgcacggccg
      541 gcgcggcaac atggaacacc tcgccatctt cgacgccgta tgcgccaacg gcgccggcag
      601 tcaacaccac gttcgtcccg ctccgccgtg caagatcgat ggcgatctcg tcgagagggc
      661 cggactcatc gacgagcgcg aacgcctcga cctcgttgac gatcaggtac tgtacgtcgg
      721 gcagcaccgg ccataccgtc gtcgggtccg gggccgcatt caggacggtc gacgccccga
      781 gctctcgtgc cgtccgtatc gccacagcgg ccacctcgct cggcacctcg ttcggctgca
      841 ggtagatagc tggtcgatgg gcttccagcg cgccccgaat ttgctccccg gtcaccctga
      901 gggtcggtcc cggcacgtag gcgatgcggt tctcgccctc gcgctccacc gtgatcagcg
      961 ccacgccgga tgccatgccg gcgaactccg tgatcccgtc aacgatgatg ccgtcggcgg
     1021 caagatccgc ccgtcgatcc ataccaaacg cgtcatcgcc cactgcgccg accattgccg
     1081 tgagcgaccc ggagcgctgt gcagcgatgg cttggttggc gcccttgcca ccgctgaaga
     1141 ttgagaacga cgagccggtg accgtctccc cggcctgggg cgctcgctcg acgaccgcca
     1201 caaggtccgt gttgatcgcc ccggcgacga cgatatcagc cttgtcgctg tctccttcca
     1261 tggcgctccc ctgccctcac acgcatcaac gcccgaaccg gacgaaccgc cggatcagcg
     1321 cgcgctgcat ctgccgaatg gcatccccgg cccgctcgag cctggttcgc tctggcgcac
     1381 ggccaccata ctggtgatcc tcgtacacat ccacgatatc gtgggcatcg cccgcgaggt
     1441 cggggtaccg gtaaccgagc tggcgcgcgt actcgcgcgg cgtctcggag gggttaccgc
     1501 ggatacctgc gaagcgtccc catcgcacca tcgcgccgaa aagggccccc gggtcgagcg
     1561 cgggtgatcg cgtcgccacc caccagacac cgctcgccag tgctgccaca gcaagcacgc
     1621 cggcgccgag cggaagcggc cagcccggac catcctcatc ctcgtcgggc gtctccggca
     1681 ccacgtctgc gatcggagcg gcggagtcat cgccgaggtc tggcgtcgcc accgggggct
     1741 cgggcgcaac tgtttcgagc tcgggcgtgg gagtcgggat ttcgtcggaa acgtccgcag
     1801 cggcggcgcc atcgagcgtg ctctccggct gcgaggcggt cggctcaaag cggatccatc
     1861 catactctgg gaagaacgct tcggtccatg catgagcctg ctgctgcctg tagatgtagc
     1921 cgttcgcctc ctcgtcccaa tcacccgggt agtaaccgac gactgtcttg gctggaatgc
     1981 cgagcgtgcg cagcatgacg gtcatcgagg tggcatagtg ctcgcagtag ccacgccggt
     2041 tctcgaagac cacataatcg acgatgtccc ggtcctctgg cgcgacgccg acctccaggt
     2101 catagacgat gtggtttcgc agaaagagct caatggcttt cgcacggtcg tacggattgt
     2161 cgatgccgga ggtga
//
LOCUS       DASYWL010000004        10788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_619377, whole genome shotgun sequence.
ACCESSION   DASYWL010000004 DASYWL010000000
VERSION     DASYWL010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10788
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619377"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /locus_tag="VGR22_00090"
     CDS             complement(<1..1094)
                     /locus_tag="VGR22_00090"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV2127000.1"
                     /translation="MPNRFAIPDVLVIGGGIAGCGLASVLARAGMSVIVVERTTRFID
                     RIRGEFAHPWGVRELERVNLLDVATARAGGRILPYWTKYTDGVPGEPYRWSDDFPDVP
                     GSLSVSHPALQQALIDHAAEQGAAVLRPAALDEITWEGDQPVVRVHADDTWHEVRPRL
                     VVGADGTHSLVRRQLGGTGITDEPHHAIGGALVAGIDLPADSAHQAYFAGGFAMVFPQ
                     GDDISRVYYVCSTEEAAVLQLADQPKALLDRLRDILPSEATRSMAGTESPVGFFPNAE
                     TLATVTHGPATVLIGDATGSNDPSQGHGLSLVFRDIHDLSNRLARESDWSVIPSTFAA
                     ERARVHGVLRAHAHWVEPLSTETGPHIDAMR"
     gene            complement(1111..1476)
                     /locus_tag="VGR22_00095"
     CDS             complement(1111..1476)
                     /locus_tag="VGR22_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127001.1"
                     /translation="MTTVHMIVGSLVVLGYLVVLVLNLRTATGKAMLSIQRPLSMTAA
                     ALYLLQIMIGFSLLGEGAEIPGTHFLIALLAIFSIGAEHMLTGRETDSRRAGRIGAIT
                     TGITFAIVLAAYLIGETNS"
     gene            1778..3754
                     /gene="acs"
                     /locus_tag="VGR22_00100"
     CDS             1778..3754
                     /gene="acs"
                     /locus_tag="VGR22_00100"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEV2127002.1"
                     /translation="MTDQKQPHVVAIEALYAEDRTFAPSDAFREQANISDPEIYERAA
                     ADPEAFWAEQAESLSWYRKWDTVLEWDEPFAKWFVGGKLNASYNCLDRHVEAGNGDRV
                     AYYWEGEPGDTVQITYSELLTEVCKAANALKELGVERGDRVAIYMPMILELPIAMLAC
                     ARIGAPHSVVFGGFSSDALRDRINDAEAKLVITADGGFRRGAPSTLKPAVDVALGETE
                     SVEKVLVARRTKEDVPMVEGRDVWWHDTVDRQDETCEPEQMESEDVLYVLYTSGTTAK
                     PKGIVHTTAGYLLGTSWTHQSVFDLKPDSDIYWCAADIGWVTGHSYIVYGPLANGATS
                     VMYEGTPTYPAPDRWWDIIERYKVTILYTAPTAIRAHMKEGHQHAEKHDLSSLRVLGS
                     VGEPINPEAWMWYHRYIGREQAPIVDTWWQTETGQILISPLPGITTTKPGSATFPLPG
                     IDADIVDDQGESVPRGEGGYLVVKKPWPAMLRGIYGDLERYKETYWSRFPHMYFPGDG
                     AKRDEDGYFWLLGRVDDVMNISGHRLSTTEIESALVSHDFVAEAAVVGYPDDVTGEAI
                     FAFVTLKSRAHVNKEFGAELRNYVGEKISPIAKPKQLMFTPDLPKTRSAKIMRRLLRD
                     IASGHALGDTTTLADPGVVETIREATQTASAEGE"
     gene            complement(4152..5225)
                     /locus_tag="VGR22_00105"
     CDS             complement(4152..5225)
                     /locus_tag="VGR22_00105"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV2127003.1"
                     /translation="MSDSTTHEPPTPRRVLITGGAGFLGINLARYLACRGVEVISLDL
                     APFDYEDMQDTVTAITGDIRDPQAVARALEGCDAVVHTAAALPLYTSEDIYTTDVEGT
                     RTVLSCARLAGIDRGVHISSTAVYGIPDHHPLLEDDRLEGVGPYGQAKIQAEMVCLEA
                     RARGMTVPILRPKSFVGPERLGVFALLYNWALEGRNFPMIGSGNNRYQLLDVEDLCVA
                     IWLTLTLPREAINDTFNIGAAEFTTMKQDYQAVLDAAGHGKRIIGFPATPMVWTLRLL
                     DRLGVSPLYRWVYETAGKDSFVSTEKAERQLGWQPKYSNQEALLRNFAWYREHRDRFA
                     GASGVSHRVPWKQGVLGLAKRLF"
     gene            complement(5236..6321)
                     /locus_tag="VGR22_00110"
     CDS             complement(5236..6321)
                     /locus_tag="VGR22_00110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEV2127004.1"
                     /translation="MQNDQNRSAHARSASIDPRYASPPMGDTGPTSALRRALPRILGG
                     VLLAVIVASALALFADVRSLGAALESFSWWLLVPVLGLTLVNYVLRWVKWHVYLRVVG
                     VPRISLADSVLAFLSAFSMALTPGKVGEIIKAIHVHRLTNSPVSRVAAVIAAERITDG
                     LAMTGLAAVGFLQFSQGRPVMAAAAVLGIGAILLFRHPSTLHTITQRLATVPVAGPLI
                     GAVLHHIEGFLDASNALYAPKLVVGSVALGMVSWFCECVALYLILIGLGIEASWTLLL
                     VSTFVLSVSSVLGALSMLPGGLGVAEASVAGLLLLLIDDPTFARADAAAATLLIRFAT
                     LWFGVLLGVVALPTLQRRGRPATMLAR"
     gene            complement(6523..6660)
                     /locus_tag="VGR22_00115"
     CDS             complement(6523..6660)
                     /locus_tag="VGR22_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127005.1"
                     /translation="MLDSIKDKTGVYRLGFNKYSFLGIVLPLLFVLVVGGGLAALIIL
                     T"
     gene            complement(6769..8328)
                     /gene="lysS"
                     /locus_tag="VGR22_00120"
     CDS             complement(6769..8328)
                     /gene="lysS"
                     /locus_tag="VGR22_00120"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEV2127006.1"
                     /translation="MELSELQQIRLDKVQRLRERGEEPYPTRAQRTHTNAEALALYEE
                     ADVAGSLVEDGVFDTPMTLVGRIVSFRHMGKTVFAHIEDGYARLQLYIRKDEVGEDRY
                     NDVLKLLDLGDFVQADGTLFRTRTGEVSLRVTGFTLLAKALNAPPEKWHGLQDIETRY
                     RQRYVDLLANEEVRQIFRTRATIITALRQFLDARNYIEVETPVLQPLYGGAAARPFTT
                     YHNALDQTFYLRIADELYLKRLLVGGLERVYEISKDFRNEGIDRNHSPEFTMLEFYEA
                     YSDYEKMMETVEALIIHVVETVFGEPRFTVGDEVIDLTPSWPRKTLRDVIGEASGIDY
                     VEHRTQAELERAARDAGADIEPGTVWPRIVDELLKQFVRPNLMQPIFLIDYPVELSPL
                     AKRKDDDPTHVERFQPYVGGAELGNAFTELNDPMDQLARFREQQEDRAAGDEEAMPLD
                     WDYINSLMYGMPPTGGVGIGIDRLTMLLTGQQTLRDVILFPAMRPLPAETEAEIQETP
                     VGSVKSESPAD"
     gene            complement(8438..8908)
                     /gene="greA"
                     /locus_tag="VGR22_00125"
     CDS             complement(8438..8908)
                     /gene="greA"
                     /locus_tag="VGR22_00125"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEV2127007.1"
                     /translation="MSTERKVRLTAEGKDRLEQELDHLRNERIPELSRMIQEMTGDGD
                     VSDNSEYEEMKDEYMHLENRVYDLETTLEHAEIITPGSGDGSVQLGSKVTVRDDDGIE
                     EEWLIVDSYEARSSENRVSDDSPVGKALIGCRAGDAATVETPGGAFTYTIVDVE"
     gene            9319..10071
                     /locus_tag="VGR22_00130"
     CDS             9319..10071
                     /locus_tag="VGR22_00130"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEV2127008.1"
                     /translation="MGIAFTTAKSGTRSFAPWRWLLMTLAALVLLGGAGWMSPEDARA
                     GQAATVVVDGAPLFAEHNDHTVIDWMAGGTRVDFFYGPYKGLYEIRYYGTVGWTWVEN
                     VVLGGQVAPGSGGGSGGSGSPGGERWIDVDRSNGAVRLYEGNTVLYTFWGSLSRSQGE
                     DFYATATGTYYVYTKHLPLVYTPYADNYFTHWVGFDPERRNGFHSYVKYADGSLHPNG
                     GGYTAGCVAIPPGDIDILYNFAYMGMRVVVHW"
     gene            complement(10320..>10788)
                     /locus_tag="VGR22_00135"
     CDS             complement(10320..>10788)
                     /locus_tag="VGR22_00135"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV2127009.1"
                     /translation="VLATLYYFRREITTISFDRPFAAATGLHVWRYDQLFLVLLALTI
                     VTGLQIVGNLLILAMLITPAATARLLTDRLPVMVALAGGIGALSGVAGLYISWYQQVA
                     SGAAIVLAATALFFVAYLFAPSNGIITPRVRHALHHPNPERRRPVDVSNEHVP"
BASE COUNT         1859 a         3352 c         3757 g         1820 t
ORIGIN      
        1 cgcatggcat cgatgtgcgg accggtctcg gtcgatagcg gctccaccca gtgggcgtgg
       61 gcgcgaagca ctccgtgaac acgcgctcgc tcggccgcga acgtcgacgg gatgaccgac
      121 cagtccgact cgcgcgcgag ccggttcgac aggtcatgga tgtcgcgaaa gacaagtgag
      181 aggccgtgtc cctggcttgg gtcgttcgac ccggttgcgt cgccaatcag cacggtggcg
      241 ggaccgtggg tgacggtggc gagcgtctcg gcgttgggga agaagccaac gggactctcg
      301 gtgccggcca tgctgcgggt cgcctcgctt ggcaggatgt cgcggagccg atcgagcagg
      361 gccttcggct ggtcagcgag ctgcaggacg gctgcctctt cggtggagca gacatagtag
      421 acccggctga tgtcgtctcc ctgggggaag accatcgcga agccgccggc gaagtaggcc
      481 tggtgggcgg aatcggcggg gaggtcgata cccgccacca gcgcgccgcc gattgcatga
      541 tgcggctcgt cggtgatccc ggtgccgccg agttgccggc ggaccagcga gtgcgtgccg
      601 tcagcgccga ccacgaggcg cgggcgcacc tcgtgccatg tgtcgtcggc gtggacgcga
      661 accaccggct ggtcgccctc ccaggtgatc tcgtccagtg cggcaggccg aagcacggcc
      721 gccccctgtt cggcggcgtg gtcgatcagc gcctgttgca gtgccgggtg ggagacgctg
      781 agggagccgg gcacatccgg gaagtcatcc gaccagcggt agggctcgcc cggcacgccg
      841 tctgtgtatt tggtccagta cggcaggatg cgaccaccgg cgcgtgctgt cgcgacatcg
      901 aggaggttga cccgctccag ctcgcggaca ccccaggggt gggcgaactc gccgcggatc
      961 cggtcgatga agcgtgtggt gcgctcgacg acgatgacgg acatcccggc gcgggccagc
     1021 acgctggcga ggccgcatcc ggcgatgccc ccgccgatca cgagcacatc cggaatggcg
     1081 aaccggttgg gcatggtggg gttccggtga ctagctgttc gtctcgccga tcaggtaggc
     1141 agcgagcacg atcgcgaagg tgatgccggt ggtgatcgcg ccgattcgtc cagccctgcg
     1201 ggagtcggtc tcgcggccgg tcagcatgtg ctccgcgccg atcgagaaga tcgcgagcag
     1261 agcgatcaga aagtgtgtgc cggggatctc cgcgccctcg ccgagcagcg agaagccgat
     1321 catgatctgc agcaggtaga gtgctgcggc cgtcatcgag agcgggcgct gaatcgaaag
     1381 catcgccttg cccgtcgccg tgcggaggtt caggaccagc acgacgaggt agccgagcac
     1441 gacgaggctc ccgacaatca tgtgaacggt ggtcatcagc gaggaatcct ccaggggtca
     1501 cgggtggcga ccatggcggc cgcgccggtg agcgatggac actgccatcg agtccaatgg
     1561 tccagtgtag tgcacgcggg ctgcgcagcg gcaggtgatc gggcatacct tgcaggtttt
     1621 cggggctctg gtatggtgca ggcgctgctg tcggcgaggc gatcgggctt tgtatactgc
     1681 gatgtgcaga acgatgggca acagaggggt cagtggatat cggtcaccgc gcggacagct
     1741 catcgcacca tcacgtgagc agggaggaat cagagccatg acagaccaga agcagccgca
     1801 tgtcgtcgcg atcgaggcgc tctacgctga ggatcgcacg ttcgccccgt ccgacgcctt
     1861 ccgcgagcag gcgaacatct ccgatccgga gatctacgag cgcgcggcag ccgaccccga
     1921 ggcgttctgg gccgagcagg ccgaatcgct ctcctggtac aggaagtggg acaccgtgct
     1981 ggagtgggac gagcccttcg cgaagtggtt cgtcggaggc aagctgaacg cttcctacaa
     2041 ctgcctcgat cgtcatgtgg aggcgggcaa cggtgaccgc gtcgcctact actgggaggg
     2101 cgagccgggc gacaccgtcc agatcaccta cagcgagctc ctcaccgaag tctgcaaggc
     2161 tgccaacgcg ctcaaggagc tcggcgtgga gcgcggcgac cgtgtggcca tctacatgcc
     2221 gatgatcctc gagctgccga tcgcgatgct cgcctgcgcc cgcatcgggg cgccgcactc
     2281 ggtcgtcttc ggcgggttct cctccgacgc cttgcgtgac cgaatcaacg acgccgaggc
     2341 gaagctggtg atcaccgccg acggcgggtt ccgccgcggc gcaccctcca cgctcaagcc
     2401 ggcggtcgat gtcgcactcg gggagacgga gagcgtcgag aaggtcctcg tggcccggcg
     2461 cacgaaggaa gatgtgccga tggtcgaggg tcgagatgtc tggtggcacg acaccgtcga
     2521 ccggcaggac gagacctgcg agccagagca gatggagagc gaggacgtgc tgtacgtgct
     2581 ctacacgtcc ggcaccacgg ccaagcccaa gggcatcgtt cacaccactg ccggctacct
     2641 gctcggcacg agctggacgc accagagtgt tttcgatctc aagccggatt cggatatcta
     2701 ctggtgcgcg gccgacatcg gctgggtgac cggacactcc tacatcgtct acggcccgct
     2761 cgccaacggc gcgaccagcg tgatgtacga gggcacaccg acctacccgg cgccggaccg
     2821 ctggtgggac atcatcgagc gctacaaggt gaccatcctc tacaccgcac cgaccgcgat
     2881 ccgtgcacac atgaaggaag gccaccagca cgcagagaag cacgacctct cctcgctccg
     2941 cgtgcttggc tcggtcgggg agccgatcaa cccggaggcg tggatgtggt accaccgcta
     3001 catcggccgc gagcaggcgc ccatcgtcga cacctggtgg cagaccgaga ccgggcagat
     3061 cctcatctct ccgctgccgg gcatcacgac caccaagccg ggctcggcga ccttcccgct
     3121 gccgggtatc gacgccgaca tcgtcgatga ccagggcgaa tcggtcccgc gcggtgaggg
     3181 tggctatctg gtcgtcaaga agccgtggcc ggcgatgctg cggggtatct acggtgatct
     3241 ggagcgatac aaggagacct actggagccg gttcccgcac atgtacttcc ccggcgacgg
     3301 ggccaagcgc gacgaggacg ggtacttctg gctgctcggc cgcgtcgacg acgtgatgaa
     3361 catctccggg caccggctca gtaccaccga gatcgagagc gcactggtct cgcacgactt
     3421 cgtcgccgag gcggcggtgg tcggctaccc ggacgatgtt accggtgagg cgatcttcgc
     3481 gttcgtcaca ctgaagtcgc gcgcgcacgt caacaaggag ttcggtgccg agctgcggaa
     3541 ctacgtcggc gagaagatca gcccgatcgc caagccgaag cagctgatgt tcacgccgga
     3601 cctgccgaag acgcggtcgg cgaagatcat gcggcggctg ctgcgtgata tcgcatcggg
     3661 ccacgccctc ggcgacacca cgaccctggc cgaccccggt gtggtggaga cgatccgtga
     3721 ggcaacgcag accgcctccg ccgaaggcga gtaggcgtcg gcggaggcgc tccatgccgc
     3781 tcggcagatg ggctattcgt tcgagcggat ccgcgtcgtg accggaccac caacgcatct
     3841 gtcatcctga gcctagcaaa ggatcccccg cccgtggcac cgccatgggc ggtttctctg
     3901 gcgtgacacg cgcacacgag cgggaccgta ccggtccggg atcgccagga aacgctggtt
     3961 gaacattgat cgggcaaaga aaacgaccgg ccggcgcgtg ctccggccgg ggattcttcg
     4021 aatcggccgc ggcgcatgcg cgccttgccg gctcagaatg acaattcttt cggggtatgg
     4081 cactcgacgg ctggccgcca gcgcagggcg ggcggacaca aggtccgccc ctacggcttg
     4141 gaagcggtcc gtcagaacag gcgcttggcg aggccgagga cgccttgctt ccagggcacc
     4201 cgatgcgaga caccgctggc gccggcgaac cggtcccggt gctcccggta ccaggcgaag
     4261 ttgcgcagca gcgcctcctg gttgctgtac ttcggctgcc agccgagctg tcgctccgcc
     4321 ttctcggtgc tcacgaagga gtccttgccg gccgtctcgt agacccatct gtagagcggc
     4381 gagacgccca gccggtcgag caggcgcagc gtccagacca tcggtgtggc ggggaacccg
     4441 atgatgcgct tgccgtgccc ggcggcatcg agcaccgcct gatagtcctg cttcatggtc
     4501 gtgaactccg ccgcgccgat gttgaacgtg tcgttgatgg cctcgcgggg cagcgtcagg
     4561 gttagccaaa tcgcgacgca gagatcctcc acatcgagca gttggtaccg gttgttgccg
     4621 ctgccgatca tcgggaagtt gcgaccctcc agcgcccagt tgtagagcag cgcgaagacc
     4681 cccagccgct cggggccgac gaagctcttc ggccgcagga tcggtacggt catgccgcgc
     4741 gcccgcgcct ccaggcagac catctccgcc tggatctttg cctgtccgta cggcccgacg
     4801 ccctcgagcc ggtcgtcttc gagcaatggg tgatggtcgg ggatgccgta gacggcggtc
     4861 gaggagatgt ggacgccccg gtcgatgccg gccagcctgg cgcaactgag gaccgtgcgg
     4921 gtgccctcga catcggtggt gtagatgtcc tcggatgtgt agagcggcag cgcggcggcg
     4981 gtgtgcacta cggcgtcgca gccctccaac gcgcgcgcga ccgcctgcgg gtcgcggatg
     5041 tcgccggtga tggccgtcac cgtgtcctgc atgtcctcgt agtcgaaggg ggcgaggtcg
     5101 agcgagatga cctcgacgcc gcggcaggcg aggtaacgcg ccaggttgat ccccaggaag
     5161 ccggcgccgc cggtgatcag cacccggcgg ggggtgggtg gttcatgcgt agtggaatcg
     5221 gacacgtgca tcctctcagc gggcgagcat ggtcgccggt cggccacggc gttgcagggt
     5281 ggggagcgcg accacgccga gcagcacgcc gaaccagagg gtggcgaacc ggatcagcag
     5341 ggtcgccgcc gcggcgtccg cgcgtgcgaa tgtcgggtcg tcgatcagca ggagcagcag
     5401 acccgccacg ctcgcctccg caacgccgag gccgcccggc agcatggaga gcgccccgag
     5461 cacagatgat acggagagca cgaaggtgct gaccagcagc agcgtccagc ttgcctcgat
     5521 ccccagcccg atcaggataa ggtagagcgc cacgcactcg cagaaccagg agaccatgcc
     5581 cagcgcgacc gagccgacga ccagcttcgg tgcgtagagc gcgttggagg cgtcgaggaa
     5641 accctcgatg tggtggagca cggcaccgat cagcggcccg gccacgggca cggtggccag
     5701 ccgctgggtg atggtgtgca gggtcgaggg atgtcggaac agcagaatcg cgccgatgcc
     5761 cagcacggcg gcggcggcca tcaccggccg gccctggctg aactgcagaa agccgacggc
     5821 ggcgaggccg gtcatcgcca gcccgtcggt gatgcgctcg gcagcgatca ccgccgcgac
     5881 ccggctcacc ggcgagttgg tgagccgatg gacgtggatc gccttgatga tctcgccgac
     5941 cttgccgggc gtgagggcca tgctgaacgc ggagagaaag gcgaggacgc tatcggcaag
     6001 cgagatgcgg ggaacgccga caacgcgcag gtagacatgc cacttgaccc agcggagcac
     6061 gtagttgacg agggtcaggc cgagcaccgg cacgagcagc caccagctga agctctccag
     6121 ggcggcgccg agcgagcgga cgtcggcgaa gagcgcgagc gccgatgcga cgatgaccgc
     6181 gagcagcacc ccgccaagga tgcgcggcag ggcccggcgc aaggcggatg tggggccggt
     6241 gtcacccatg ggcggcgagg cgtagcgcgg gtcgatcgat gctgagcggg cgtgagcgga
     6301 gcggttctgg tcgttctgca tcgatgaggc tcattcttct cactggcggt gtggtgcgtt
     6361 ggcatttgaa tccgcagcag ggtggacatg gggcatcgag ggcaaacggg cgaagcgagc
     6421 gcctcgtggc atgcgcgcgt ctgggccacc cgcccggatg cgcgcaacgt gaagccccct
     6481 ggcaggttgc gccgaggggc cgtatcactg acgggttagg tcctaggtca ggatgatgag
     6541 cgcggcgagt ccgcccccga ccacgagcac gaagagcagc ggcagcacga taccgagaaa
     6601 tgaatacttg ttgaagccga gcctgtagac accggttttg tccttgatgc tgtcgagcat
     6661 gtgatcgtcc tttcttcgct tcgttcgcga ggcccctgtc gcggagatca cggcacggag
     6721 acgctccttc ccgagggtct cccttcattt ttgtattgta gtggagcgtc aatccgctgg
     6781 cgactccgat ttgaccgaac ccacaggcgt ttcttggatc tctgcctccg tctcggcggg
     6841 aagcggacgc atcgccggga agagaatcac gtcgcgcagg gtctgctggc cggtcaggag
     6901 catcgtcagg cgatcgatgc cgatgccgac gccgccggtc ggcggcatgc catacatgag
     6961 cgagttgatg taatcccagt cgagcggcat ggcttcctcg tcaccggcgg ccctgtcctc
     7021 ctgctgttcc ctgaaccggg cgagttggtc catcgggtcg ttcagctcgg tgaaggcgtt
     7081 tccgagctcc gcgcctccga cgtagggttg gaagcgctcg acgtgcgttg ggtcatcatc
     7141 cttgcgcttc gcgagcggcg agagttctac cgggtagtcg atcaggaaga tcggctgcat
     7201 caggtttggc cgcacgaact gcttgagcag ctcgtcgacg atgcgcggcc agacggtacc
     7261 cggttcgatg tcggcgcccg cgtcgcgcgc ggcacgctcg agctcggcct gggtcctgtg
     7321 ctcgacgtag tcgatcccgc tcgcctcgcc aatcacgtcg cgcagggtct tgcgcggcca
     7381 tgatggcgtg aggtcgatta cctcgtcgcc gacggtgaag cgcggctcgc cgaagacggt
     7441 ttccacgacg tggatgatga gcgcctcgac ggtctccatc atcttctcgt agtcgctgta
     7501 cgcctcgtag aactcgagca tcgtgaactc gggcgagtgg ttgcggtcga tgccctcgtt
     7561 gcggaagtcc ttcgaaatct cgtagacgcg ctccagcccg ccgacaagca gccgcttcag
     7621 gtagagctcg tcggcgatgc ggaggtagaa ggtttggtcg agtgcgttat ggtaggtcgt
     7681 gaacggccgg gcggccgcgc cgccgtagag cggttgcagc accggcgtct cgacctcgat
     7741 gtagttccgc gcgtcgagga actgccggag cgcggtgatg atcgtcgctc gcgtgcggaa
     7801 gatctggcgt acctcctcgt tggcgaggag atcgacgtag cgttgccggt agcgggtctc
     7861 gatatcctgc aggccgtgcc acttctcggg cggggcgttc agcgccttgg cgagcagggt
     7921 gaagccggtc acgcgcagcg agacctcacc cgtacgggtg cggaagagcg tgccatcggc
     7981 ctggacgaag tcaccgaggt cgagcagctt gaggacatcg ttgtaccggt cctcgccgac
     8041 ctcgtcctta cggatgtaga gctgcaggcg ggcgtagccg tcctcgatgt gcgcgaacac
     8101 cgtcttgccc atgtgccgaa agctgacgat gcgcccgacg agcgtcatgg gcgtgtcgaa
     8161 tacgccgtcc tcgacgagtg agccagcgac gtcggcctcc tcgtagagcg cgagcgcctc
     8221 ggcattggtg tgcgtgcgtt gtgcccgggt ggggtagggc tcctccccgc gctcgcgcag
     8281 gcgctgcacc ttgtcgagtc gaatctgctg cagttcgctc agctccatga gggtctgtct
     8341 ccgcgtgaca tggtgacggg ttgcgggtcc gttccgaaca tgccgaacgc ggcgggagta
     8401 ctcccgccgc gttcgatcat gcagccggaa ttgctgacta ttctacgtcg acgatggtgt
     8461 aggtgaaggc gccgccgggc gtttcgacgg tggcggcgtc accggcgcga cacccgataa
     8521 gggccttgcc aacaggggag tcatcggaga cgcggttttc ggaggatctg gcctcgtagg
     8581 agtcgacgat gagccactcc tcttcgatgc cgtcgtcgtc gcgaaccgtg accttcgagc
     8641 cgagctgaac gctaccgtcc ccgctaccag gcgtgatgat ctcagcatgc tcgagggtcg
     8701 tttcgagatc atacacccgg ttctccagat gcatgtactc gtccttcatc tcctcgtact
     8761 cgctgttgtc cgagacgtca ccgtcgccgg tcatctcctg gatcatgcga ctgagctcgg
     8821 gaatgcgctc attgcgcagg tggtcgagct cctgctccag acgatccttg ccctcggcgg
     8881 tcaggcgaac cttccgttcc gtgctcatgt ggtgttcctg cctttctgca cgccggctgc
     8941 accgggtatg tgctcgtaac gtgtacgaca gagcacacag ggtgtgctcc ggagaaccag
     9001 ccctgcaaaa cgcaataggt atgcaataga gccggagggg cctgcctccg gctcggctcg
     9061 gttgctgcca gtatacggca accggcctga acgcgccccc cttatgcccg cctagcgaat
     9121 cgaaggcggc gtccgctgga atgtcgtgac catgagtcac agacgccaaa aggcccgatt
     9181 ctcacagccg cttgcaatca ttctcctctg tctgtatagt acgaccacag tgtacgtaca
     9241 ctgctcgcat ccggggcagt ggcagggagg ggaaggggcg ccggggcgtc ccgacgatgt
     9301 acggtcagga gggcctgaat gggaatcgcg ttcacgacag caaagtccgg aacgaggtcg
     9361 ttcgcaccgt ggcgatggct gctgatgacg ctggctgcac tcgtgctgct cggcggcgca
     9421 ggatggatga gccccgagga tgccagggcg ggccaggcag cgaccgtggt ggtggatggt
     9481 gcgccgctgt ttgccgagca taacgaccac acggtgattg actggatggc ggggggcacg
     9541 cgcgtcgatt tcttctacgg accgtacaag gggctgtacg agattcggta ctacggcaca
     9601 gtcggatgga cctgggtcga gaatgtcgtg ctcgggggcc aagtcgcgcc gggcagcggt
     9661 ggtggcagcg gagggtccgg ttcaccgggc ggcgagcgtt ggatcgacgt cgatcgatcc
     9721 aacggtgcgg ttcggctcta tgagggcaac acggtgctct acaccttctg gggctcactg
     9781 agccgcagtc agggcgagga tttctatgcg acggcgacgg gcacctacta cgtctacacc
     9841 aagcacctgc cgctggtgta cacgccgtat gccgacaact acttcaccca ttgggtgggc
     9901 tttgacccgg agcggcgcaa cgggttccac tcctacgtga agtacgccga tggttcgctt
     9961 cacccaaatg gtggcggcta caccgccgga tgcgtggcga ttccgccggg cgacatcgac
    10021 attctctata acttcgcgta catggggatg cgagtggtgg tgcactggta gagggcaggc
    10081 tgccctttcc gcgatccgaa cccaatggaa atcgctggaa tccgggtggg gatcaggact
    10141 cacggttccc gcccggttct gccatatgtg gcagccctcg tgccgtcatg ctggtacgga
    10201 cttcaggtga tcccttggtc gagccgaggt ggaggagcgg aaaccgtccg cgcacgtcca
    10261 ttgggaggat cttgtgtgaa ttaatccgta ggggcggatc gtgtgtccgc ccccggtcgt
    10321 tacgggacgt gctcgttgct gacgtcgacc gggcgccgcc gctccgggtt cggatggtgt
    10381 agtgcgtggc gcacgcgtgg cgtgatgatc ccgttggatg gcgcgaacag ataggcgacg
    10441 aagaagagcg ccgttgccgc aagcacgatc gccgcgcccg acgccacctg ctgataccaa
    10501 gagatgtaga ggcccgccac gccggagagc gcgccgatgc cgcccgccag cgccaccatc
    10561 accggcagcc ggtcggtcag cagccgcgcg gttgccgccg gggtgatcag catcgcgaga
    10621 atcaggaggt tgccgacgat ctgcagcccg gtcacgatcg tcagcgccag cagcacgagg
    10681 aagagttggt cgtagcgcca gacgtgcaac ccggtcgccg ccgcgaaggg gcggtcgaac
    10741 gagatggtcg tgatctctcg gcggaagtag tagagcgtcg ccagtacc
//
LOCUS       DASYWL010000005         6948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_656952, whole genome shotgun sequence.
ACCESSION   DASYWL010000005 DASYWL010000000
VERSION     DASYWL010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6948
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656952"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="VGR22_00140"
     CDS             complement(<1..768)
                     /locus_tag="VGR22_00140"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEV2127010.1"
                     /translation="MTHPFTTALAQRPLLFDGAMGTMLYEAGRSLDESLDALNLTHPD
                     LVANIYRAYINAGADVIETNTFGANAIRLEQHGLADKVRDINFRGVKIAREEREISGR
                     PVLVAGSVGPTGRTLAPIGTLQREQATAAFRDQIDALLEAGVDLLVFETMGSMEEMLA
                     ALDAAEQCADLPIVALMTFAEDGRTLSGHNPADVAPVLADRGVNVIGANCSVGPQRLM
                     NIIRKLRATLEDRDGNRPAVAAVPNAGYPTVVGSRLMY"
     gene            1022..1873
                     /locus_tag="VGR22_00145"
     CDS             1022..1873
                     /locus_tag="VGR22_00145"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HEV2127011.1"
                     /translation="MVDTANTRTKREQDDAADEHTRPGALMAIGGAEDKLDDMVILSR
                     FVRMAGGKKARVVIVPTASSFEDAGLRYKAIFLGMGVEEAEVTFLQSRDDANDSEHID
                     ALEDATGIFLTGGNQVKLSVMIGGTQFEETVRRKHRSGTVIAGTSAGASILSAHMVAG
                     GAGGAVPKQRMAQMVAGFGLISNVIIDQHFRQRNRIGRLLALVATNPRLLGVGIDEDT
                     AAIFHDGRLEVIGRHSVTVVDGSEMYTDIFRVKGHGEVTVSNARLHVLPAGRTFDLES
                     RTLSEDL"
     gene            1997..4633
                     /gene="cphA"
                     /locus_tag="VGR22_00150"
     CDS             1997..4633
                     /gene="cphA"
                     /locus_tag="VGR22_00150"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: protein motif:HMM:NF010623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEV2127012.1"
                     /translation="MMEIRKTTVYRGPNVWARMPAIQYVLDLGELEDRPSNKIPGFYE
                     QLTELMPSLYEHRCSVGRPGGFLQRLREGTWMGHVLEHVALEIQNLSGADVGRGKTRS
                     TTEKGVYNVIFQYDQEDVGLAAGKMALRLLNNLVYDKPEDFDFEAELEQVIRLAERLA
                     YGPSTGSIVQEAQRRGIPVLRLDPRRSLVQLGHGKYQKRIWATVTSETANIAVDIASN
                     KELTNRLLQEVGIPVPKSATVGNAEDAVRSAERIGYPVVLKPLDGNHGRGVFVNLADE
                     GEVREFFESAHSESRSGIVVVESFITGRDYRILVVNNDVIAVSERVPAHVVGDGKTTI
                     AELIERENADPRRGVGHEKILTRITVDSQTMEVLERDGRSLDDVPDEGEFVQLKLTGN
                     MSTGGTAIDRTDEIHPDNVEIARQAAMVVGLDIAGIDFITTDIAESVRQTGGAICEVN
                     AGPGFRMHTNPTEGHPRHVGRAVVDMLFERGKPSRVPIVAVTGTNGKTTTSRMIAHIM
                     KTAGYKVGLTTTDGIYIDGTQILSGDMSGPGSARMVLKNPAIDYAVLETARGGILRSG
                     LGYDRCNIAVVTNIASDHLGLKGIHTLAELARVKAVVPQSALRDGASVLNADNEWTVQ
                     MSRTARGEIIYFSMDEENPVIQEHIREKGKAVVLRQSRYGEMITVVENRRETSLVLAS
                     QIPATFEGRVRVNIANALAASAVALADDVPLDHIRQALRTFSSSFFQTPGRFNLLDLG
                     GQRVVMDYCHNVAGLEALGDFVSRMEADRSVAVISIPGDRSDEDMRQFGELAASVFDR
                     IIIREDDNRRGRQIGEIADRLKAAAIAGGKPQQDIEIVLDEMEAARHAVDTTSRDELV
                     VILADKPVKVWQMLTARKDAVI"
     gene            4635..5174
                     /locus_tag="VGR22_00155"
     CDS             4635..5174
                     /locus_tag="VGR22_00155"
                     /inference="COORDINATES: protein motif:HMM:NF002598.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyuridine 5'-triphosphate
                     nucleotidohydrolase"
                     /protein_id="HEV2127013.1"
                     /translation="MTELTGLIGGVLSREELLSRIVRPDRPLLRDYVSLEEQVQPNGI
                     DLTLAKVERFAGSGKIAMEGSGRELPKLEEVHPDERGWFHLEAGPWHITYNEVVALPD
                     DLMALGRPRSSLGRSGVAIHTAVWDAGYEGRSTSLLQVVNPSGFSVQRNARVMQLVFV
                     TLNARTAAGYSGQYQGENI"
     gene            complement(5246..5527)
                     /locus_tag="VGR22_00160"
     CDS             complement(5246..5527)
                     /locus_tag="VGR22_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127014.1"
                     /translation="MPSLLDVILRRNTELKTPPDCQVHDVEMDLRGKMGRPSRFDNMS
                     EEDYTFIYYCPVDGCNETAERHVVRREIPVPGVTPKRPDFTPTKDRVKY"
     gene            5610..6089
                     /locus_tag="VGR22_00165"
     CDS             5610..6089
                     /locus_tag="VGR22_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127015.1"
                     /translation="MKTSQEAVEAPQAPLRHLYEVVLVTLQGALGLSWGKPGKSVRQV
                     FRRIRETVADLDGIHLAADLDHDDQAGLHIMLWVDRDDFDLLLADDIANEIIRSLGDR
                     EVLLACRSLEDSGIRYRFASGSVERGMTGAVMLVGPYAQDVSRLARIGIGQPTGFSA"
     gene            6136..6498
                     /locus_tag="VGR22_00170"
     CDS             6136..6498
                     /locus_tag="VGR22_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEV2127016.1"
                     /translation="MPTVRVEGEAPFEAEEGRRLVLAIEDAGIDILHRCGGNARCTTC
                     RVEVLEGNPGPMTEAEEKRLETVSDRTDDTRLSCQVQVRDDLAVSVKNRLHEKPELGD
                     AGPRPIEWPKDHPLPPEE"
     gene            6545..6807
                     /gene="ffs"
                     /locus_tag="VGR22_00175"
     ncRNA           6545..6807
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VGR22_00175"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
BASE COUNT         1285 a         2107 c         2344 g         1212 t
ORIGIN      
        1 gtacatcaga cgggagccga cgacggtcgg gtagcccgcg ttcggcactg ccgcaaccgc
       61 cggccggttc ccgtcgcggt cctccagcgt agcgcgcagc ttgcggatga tgttcatgag
      121 tcgctgagga ccaaccgagc aattggcacc gatcacattg acgccccggt cggcaagcac
      181 cggagcgacg tcggctggat tgtggcccga gagggtgcgg ccgtcctcgg caaacgtcat
      241 tagggccacg atcggcaggt cggcgcattg ctcggcggca tcgagcgccg ccagcatttc
      301 ctccatgctg cccattgtct cgaagacgag caggtccaca cccgcctcca gcagcgcatc
      361 gatctggtcg cggaacgcag ctgtcgcctg ctcacgctgc agcgtgccga tcggcgcaag
      421 ggtgcgaccg gtcggaccga cggagccggc aactagcacc ggtcggccgc tgatctctcg
      481 ctcctcgcga gcgatcttga ccccgcggaa gttaatgtct cggaccttgt cggcaagccc
      541 gtgctgctcc agccggatcg cgttggcccc gaaggtgttg gtctcgatca cgtccgcgcc
      601 ggcgttgatg tatgcgcgat agatgttggc aaccaggtca gggtgggtga ggttaagcgc
      661 gtccagggac tcgtcaagcg acctcccggc ctcgtagagc atggttccca tcgcaccgtc
      721 gaagaggagc gggcgctggg ccagcgcagt cgtgaacggg tgggtcatgg ggtgagatgc
      781 tcctgcagtg aacgatcgac cgtggtagcc gctgacccgg ttcaatctca tgtcgtcgtg
      841 ctgacagtct agaatatccg gacgcgaggg gggcgtgctt cgtatcgcaa cccatcgatt
      901 gacacgtact ccacagcccc gtaaggttca tcgaatcgac acgagctatg gctcacggag
      961 gggcgcagcc ccgggtcccg agcgcctgcg ggttgcggca ggtttggcag cgaacacaac
     1021 catggttgac acagcgaaca ccaggacgaa gcgcgaacag gacgatgctg cggatgagca
     1081 tacgcggccg ggcgcgctca tggccattgg tggtgccgag gacaaactcg acgacatggt
     1141 catcctctcc cgcttcgtgc gcatggccgg gggcaagaag gcgcgcgtcg tcatcgtgcc
     1201 gaccgcgtcg tcgttcgagg atgcagggct tcgctacaaa gcgatctttc tcggcatggg
     1261 cgtcgaggag gccgaagtca cgttcctgca gtcgcgggac gacgccaacg acagcgaaca
     1321 cattgacgcg ctcgaggacg caaccggcat cttcctgacc ggcggcaatc aggtgaagct
     1381 gtccgtgatg atcggcggta cccaattcga ggagaccgtt cgtcgcaagc accgaagcgg
     1441 cacggtcatc gcggggacca gcgccggggc gtcgattctc tctgcgcaca tggtggccgg
     1501 cggggctgga ggcgccgttc ccaagcagcg catggcacag atggtcgctg gcttcggcct
     1561 gatcagcaac gtgatcatcg accagcactt ccgccagcga aaccgcatcg ggcgcctgct
     1621 ggcgctggtc gcgaccaatc cccggctgct tggcgtcggt atcgacgagg acaccgccgc
     1681 gattttccat gatggtcgtc tcgaggtcat cggtcgccac agcgtgaccg tcgtcgacgg
     1741 ctccgagatg tacaccgaca tcttccgggt caaggggcat ggcgaggtca cggtcagcaa
     1801 cgcccggctc cacgtgctgc ctgcaggccg cacgttcgat ctggagtccc gaacgctctc
     1861 cgaggacctc tagtcggata gtatgtttcc agcgtgtagg attcatatca gccagtgaca
     1921 tgggtgggcg tcgccggtgc ctgacctcca tgacggagcg caagtcccga gcccagtagt
     1981 tcacgggagt ggggccatga tggagattcg caagacgacg gtctatcggg gccccaacgt
     2041 gtgggcccgc atgcccgcca ttcagtatgt gctcgatctg ggcgagctcg aggatcggcc
     2101 ttccaacaag atccccggat tctacgagca actgaccgaa ctgatgccgt cgctgtacga
     2161 gcaccggtgt tcggtcggcc gccccggcgg cttcctccag cgcctccgcg agggcacgtg
     2221 gatgggccac gtgctcgagc acgtggcgct ggagatccag aacctctccg gcgcggatgt
     2281 tggccgcggc aaaacccgta gcacgaccga gaagggcgtc tacaacgtta tcttccagta
     2341 cgatcaggag gatgtcggcc tggcggccgg caagatggcg ttgcggctgc tcaacaacct
     2401 cgtctacgac aagcctgagg acttcgactt cgaggcggag ctcgaacagg tgatccgtct
     2461 cgccgagcgc ctcgcatacg gcccgtccac cggatcgatt gtgcaggagg cgcagcgtcg
     2521 tggcatcccg gtgctgcggc tcgacccgcg tcggtcgctc gtccagctgg gccatggcaa
     2581 gtaccagaag cgtatctggg cgacggtcac ctccgagacg gcgaacattg ccgttgatat
     2641 cgccagcaac aaggagttga ccaaccggct gttgcaggaa gtcggcatcc cggtgccgaa
     2701 gtccgccacg gttggcaatg ccgaggacgc ggtgcgttcc gcggagcgta tcggctatcc
     2761 ggtggtgctc aagccgctcg acggcaacca tggccgcggc gtgttcgtga acctcgcgga
     2821 cgagggcgag gtgcgggagt tcttcgagag cgcgcactcg gagagccgca gtggtatcgt
     2881 cgtggtcgaa tccttcatca cggggcgcga ctaccggatt ctcgtggtta acaacgacgt
     2941 catcgctgtc tcggagcgcg tgccggcgca tgtggtcggc gacggaaaaa cgacgatcgc
     3001 cgagttgatc gagcgggaga acgccgatcc ccgccgcggt gtcgggcacg agaagattct
     3061 gacgcgcatc accgtcgact cgcagacgat ggaggtgctg gagcgcgacg ggcgatcgct
     3121 ggacgacgtg cccgatgagg gcgagttcgt gcagctcaag ctcaccggca acatgagcac
     3181 gggcggcacc gcgatcgacc gcaccgacga gatccatccc gacaacgtcg agatcgctcg
     3241 gcaggcggcc atggtcgtcg ggctcgatat cgcaggaatc gacttcatta ccacggacat
     3301 cgcggagagc gttcgccaga ccggcggcgc gatttgcgag gtcaatgccg gtccgggctt
     3361 ccggatgcac acgaacccga ccgaggggca cccgcgccat gttggtcggg ccgtggtcga
     3421 catgctcttc gagcgtggca agccatcgcg cgtaccgatc gttgcggtca ctgggaccaa
     3481 cggcaagacc acgacctctc ggatgatcgc gcacatcatg aagactgccg ggtacaaggt
     3541 tggactgact accaccgatg gtatctacat cgacggcacg cagatcctct cgggtgacat
     3601 gagcggaccc ggcagcgccc gcatggtgct gaagaatccg gcgatcgatt acgcggtgct
     3661 ggaaacggca cgcggcggaa ttctgcgctc gggtctcggc tacgaccgtt gcaacattgc
     3721 ggtggttacc aacatcgcaa gcgaccatct cgggctcaag ggcatccaca cactcgcgga
     3781 gcttgcccga gtcaaggcgg tggtgcccca gagtgccctc cgcgacggcg ccagcgtcct
     3841 gaatgccgac aacgagtgga cggtgcagat gtcccgcacc gcgcgaggcg agatcatcta
     3901 cttctcaatg gacgaggaga acccggtgat ccaggagcac atccgcgaga agggcaaggc
     3961 cgtcgtgctg cggcagtcgc gctacggcga gatgatcaca gtggtggaga accggcggga
     4021 aacctcgctg gtgctcgcca gccaaatccc ggcgaccttc gagggccggg tgcgggtcaa
     4081 catcgccaat gcgctcgccg cgagcgctgt agccttggct gacgatgtcc cgctcgacca
     4141 tatccgtcag gcgctgcgca cgttcagcag cagcttcttc cagaccccgg gccgtttcaa
     4201 cctgctcgac ctcggtggac aacgggtggt gatggactac tgccacaacg tcgccggtct
     4261 cgaggcgctg ggcgacttcg tctcccgtat ggaggctgat cgaagcgtcg cggtcatctc
     4321 catccccggc gatcgttccg acgaggacat gcgccagttc ggcgagcttg ccgccagtgt
     4381 gttcgaccgg atcatcatcc gggaggatga caaccggcgc gggcgccaga tcggagagat
     4441 tgccgaccgg ctgaaagccg cggcgatcgc tgggggtaag ccacagcagg atatcgagat
     4501 cgtgctcgac gagatggagg cagcgcggca cgccgtcgac accacgtcga gagatgagtt
     4561 ggtcgtaatt ctcgccgaca agccggtcaa ggtctggcag atgctgactg cccggaagga
     4621 cgccgtgatc tgacatgacc gagctgacgg ggttgatagg cggcgtcctg agccgtgagg
     4681 agctgctgtc gcgtatcgtg cgtccggatc gcccgctgct ccgggattat gtgtcgcttg
     4741 aggagcaggt gcagccaaac ggtatcgacc tgacgctcgc gaaggtcgag agattcgctg
     4801 gaagcggcaa gatcgcgatg gagggcagcg gcagggagct gccgaagctc gaggaggtcc
     4861 acccggacga gcgtggttgg ttccatctcg aggcggggcc gtggcacatc acgtacaacg
     4921 aggtcgtggc gctgccggac gacctcatgg cgctcgggcg accccggtcg agccttgggc
     4981 gctctggtgt ggcgatccat acggcggtct gggacgcggg ctacgaaggg cgatcgacgt
     5041 cgctgctgca ggtggtgaac cccagcggat tttcggtgca gcgcaacgcc cgggtgatgc
     5101 aactggtctt cgtcacgctc aacgcgagga ccgctgccgg atacagcggc cagtaccagg
     5161 gcgagaatat ctgagccgga acggggaagc gaaagggtgg gcaccatgac ggcgtccgcc
     5221 ctgtgcgttg cggccatttc cggatctagt atttcacgcg gtccttcgtc ggcgtgaagt
     5281 cgggccgctt cggggtcacg cccggaaccg gaatctcgcg gcggacgaca tggcgctcgg
     5341 ccgtctcgtt gcagccatcc accggacagt agtagatgaa cgtgtagtcc tcttccgaca
     5401 tgttgtcgaa gcgggacggc cggcccatct tgccgcggag gtccatctct acgtcgtgca
     5461 cctggcagtc cggaggcgtc ttcaattcgg tattgcgtcg cagaatgacg tccagcagac
     5521 tcggcatcgt cgtacactcc cgggtagtga tcacagggca cgtgcatgta cctcaatcga
     5581 aagtatacgg gaagggacgc gggtccggga tgaagacaag tcaggaagcg gtcgaggcac
     5641 cgcaggcgcc gctccggcat ctctatgagg tggtgctggt gaccctgcag ggtgcgctcg
     5701 ggctcagctg gggcaagcca ggcaagtcgg tcaggcaggt cttccgtcgc atccgcgaga
     5761 cggtggccga tctggacggc atccacctgg ccgccgatct cgatcatgac gaccaggccg
     5821 gtttgcacat catgctgtgg gtcgaccgcg acgatttcga cctgctgctc gccgacgata
     5881 tcgcgaacga gattatacgg agcctcgggg accgagaggt tctgttggcg tgccgcagcc
     5941 tcgaggacag cggtatccga taccgctttg cctcagggag cgtcgagcgc ggcatgaccg
     6001 gtgcggtcat gctcgttggg ccgtatgcgc aggatgtctc tcgtctcgct cgcatcggta
     6061 tcgggcagcc gacgggattc agcgcctgag ccggcccgtg aaaccttttc gctctgatca
     6121 tcaggggagg atacgatgcc aaccgttcgg gtcgaaggag aagcgccgtt tgaagccgag
     6181 gagggacgca ggctcgttct cgccatcgag gacgccggga tcgatatcct gcaccgatgc
     6241 ggcggcaatg cccgctgcac gacgtgccgc gtcgaggtgc tggaggggaa cccgggacca
     6301 atgacagagg ccgaggagaa gcggctggaa accgtttccg atcgcaccga cgacactcga
     6361 ctctcatgcc aagtccaggt acgtgacgac cttgccgtca gtgtgaagaa ccgccttcac
     6421 gagaagcccg aattgggcga cgccgggccg cggccgatcg aatggccgaa ggatcatccg
     6481 ctacctcccg aagagtagtc tccgtgttga gattcggttg aggcaggctg tatactttgc
     6541 atcgtcgtgc tagacgggga gctggcggtg ccctgtactc gcaatccgcc ttagcgaggt
     6601 tgaattccct ctcgaggtac ggcttttcag gactgcggcc gtggcggggt gttgaagccc
     6661 gggtcccgca cggcggggaa ctgcgaatcg ggtcaggacc ggaaggtagc agccctaagc
     6721 aggattctcc gggtgatgcg gggacacccg ggtggagcca acgcgcggtc gcaagctggg
     6781 gagtccgtat cgacagaggg tgcacggcat tctctagcaa tcaggacaga aacgggcgag
     6841 gtgatatcac ctcgcccgtt tttcgtgtca acgcgttgtc gccgtttagg cgctccctgg
     6901 cgttgggaga tatcggactg accactactg cccaagtcga gcctgtgg
//
LOCUS       DASYWL010000006        10065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_525619, whole genome shotgun sequence.
ACCESSION   DASYWL010000006 DASYWL010000000
VERSION     DASYWL010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10065
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525619"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(270..1055)
                     /locus_tag="VGR22_00180"
     CDS             complement(270..1055)
                     /locus_tag="VGR22_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HEV2127017.1"
                     /translation="MHEELRERWRHQEWQVIPPRPLLPAENLALDEVLTQRVGRGERL
                     PTLRFWSWARKAIILGRFQSVQNEVYAEVAAQHEIDVVRRISGGGAMFVEPEGAITYS
                     VYVPESFVRGLSFAESYEMFDSWVLDALRDLGVNAWYQPLNDITSEGGKIGGAAQARR
                     GGAVLHHTTMAYQMTTGVLGTVLRVGQEKLSDKGIRSADRRVGPLRQQTEMERDAIIA
                     HLIEHFRSRFGLHDDELTLEEEAEMQRLVDEQFGTRDWTYFLP"
     gene            complement(1048..1341)
                     /locus_tag="VGR22_00185"
     CDS             complement(1048..1341)
                     /locus_tag="VGR22_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--protein ligase"
                     /protein_id="HEV2127018.1"
                     /translation="MHGEFKVPRGKLVVVDLDIDETSRLTDVRLSGDFFLYPEEAMTS
                     IVGALDGAPAGLPAHEYTLRVAEAVPPGTELFGFSPAAIAIAVTRAQEANADA"
     gene            complement(1356..2648)
                     /locus_tag="VGR22_00190"
     CDS             complement(1356..2648)
                     /locus_tag="VGR22_00190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV2127019.1"
                     /translation="MREQRRPVIIVGAGLAGLTAARRLHRDGISVLVLEAEPEVGGRV
                     RTRRTDDGFLIDRGFQILLSAYPALRRNVDLDVLGARPFDSGAHVWTGRRLVPLRNPL
                     NHPAGILRDLTSPVFGFGDKLRLVRWGLQTALAPWTTAAEAANVLPDQSALAALQAHG
                     FSDEFIDRFARPFWGGIALDRSLSFSAGVVRFTTKMFLAGDGVLPREGVGAVPKAIAA
                     ELPDGSVRTSTTVDALAIEGTRATGVRAAGETIEAAAVIVATDPPAAARLTGIDAIPT
                     EGVGCVTVYLATRQDPGIGKMLAIDGTGHQPVNEIAPLSAVQPSYAPDGERLLAAVLL
                     GDEPLSRDGEENGRIARESVATMLGIGDLRVVEAVAVPFSLYRQVPGIHRRLPDATTG
                     VQGLMLASDATVDASSNGAMMSGEDAAHAVRMELGTEA"
     gene            complement(2869..3309)
                     /locus_tag="VGR22_00195"
     CDS             complement(2869..3309)
                     /locus_tag="VGR22_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127020.1"
                     /translation="MLDSFQDLIEGLTETPSELREILGNPVPDDVPEDVKVMLAELGT
                     RERVQVRRVQAVMRERMPRLRAIEYEPELRALDQHIDTPEAMLTEYNNQRSELVSLLV
                     NLTLRDWDQKVDHDQQGEITLADEIENHLAWDEEMLEKFRAAMA"
     gene            complement(3316..4206)
                     /locus_tag="VGR22_00200"
     CDS             complement(3316..4206)
                     /locus_tag="VGR22_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127021.1"
                     /translation="MAYRFVLEVPESLLDEANIVVNSVPDAQILVERNSHGLGFNDPY
                     LDFSVAAHSLAVVERIYQWMANYGEPYPDVRLVLHDGRRVSFAKANVSLIVATIRRDQ
                     PWMDHTIPQIGVHEPKVWEGSGGAPAVSESQPELVLDDPASLAVRLASAPGVPVHNLG
                     PAEQYYDEVLDLRLIGRALRQDNGQLRAVEEPYDHKMARLYGREADVSFLENGPLRIN
                     LERHPRGLPLPYGVDPMEIHTTATPKHIATVRGRVLIYGNTVLESDPDTLRFVDPFNV
                     VWSITPMVAVDTTTPAALQG"
     gene            4633..5217
                     /locus_tag="VGR22_00205"
     CDS             4633..5217
                     /locus_tag="VGR22_00205"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEV2127022.1"
                     /translation="MSLFTLPSSATEGSTCASDHTHFDRFRAPIAMIIAALMLIGSLG
                     MATPAAAIELLPGPGSEQAVPPAEASSTGDAIAGFAMQFLGYPYVWAGNTPAGFDCSG
                     FTQYVVLSTVGIDIGHGTAGQTAYGTPVAWGAWQPGDLVYFANTFSAGISHAGIYIGD
                     GQMIHAENASTGVTISSIYSDYYSAHYYGAFRIG"
     gene            5522..5788
                     /locus_tag="VGR22_00210"
     CDS             5522..5788
                     /locus_tag="VGR22_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255349557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:5579..5581,aa:Sec)
                     /transl_table=11
                     /product="Rdx family protein"
                     /protein_id="HEV2127023.1"
                     /translation="MSSENGNGKGALQIEFCTSUGYEKRAASLAEHILHTRPLDVTEG
                     VTLIPSSGGVFEVTYNGELIFSKKAENRFPRREEINQAMDAATS"
     gene            complement(5902..7137)
                     /locus_tag="VGR22_00215"
     CDS             complement(5902..7137)
                     /locus_tag="VGR22_00215"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEV2127024.1"
                     /translation="MTVTSSTSEKTTPEGNTTPLAWEGQTYLVGEEIYLRPIDKDDAK
                     WSMSFRGSRFPLSPERTEEWITGDILKAEGRQHLGIRRKSDERVVGMAVIERSPIGGR
                     WLNLFVDPLFGSKIDRWRLEALRMILGWSVNEQQRPNLHAIVPASQQAFVDGAKDLGM
                     RETATFREYFFRDGQRVDAIVLEYLNSQWVSTLGDPNEVEIERIGSGEPRPVPASVRS
                     LSDPPKNAVMVGERVYLRPFTKADAEQFSIWSRRETEAEAAFNVGRYMLHKLGKWRWA
                     EGFQKQEFPEWIRFAVADRESGELAGGNGVLEVDYHDRRGETESFFLPKFRGKGYGSE
                     GKHLVLDYAFNRLDLHMVTSWVYFPNTRSAAALRKQGYREAGRINWLYSNKGTFDNFV
                     VFDYLADEWRAMPRTGEQG"
     gene            complement(7216..8448)
                     /locus_tag="VGR22_00220"
     CDS             complement(7216..8448)
                     /locus_tag="VGR22_00220"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2127025.1"
                     /translation="MTTSTPDQTNPELTRAAGQTWLVGEEIYLRPIVPADAEFVSSWK
                     RSPFPMAPERAREWIADDLAKNSNPYQKAIYLVVRRSNERPVGSVTVDNRHFPHHDVS
                     AQLDPLLGDEGFRLKTEALVLVFRMLVDEQQRPKVSIALPADETLVIEALEGIGARTT
                     TRFREKLSCPSGGRRDELVVEYLNRDWVARLGDPADVVFPRTGTGQPRPVTAPVLPDG
                     DPPTNAVRIGPRVYLRPPQKSDAEAVAHWSMREIDTSWDNGRFPVGREGVAKWFDDTQ
                     KKTPPDWIEFSVCLRETDEYLGMVGVLDVNYTHRFGESASMILNSKYREAGYGTEAKH
                     LMFDYLFNDLDVHAIQSWVMFDNPRSAAALRKQGYREAGREHWIEFRDGRFVSFVAFD
                     LLATDWRAMPRQDADIDS"
     gene            complement(8445..9680)
                     /locus_tag="VGR22_00225"
     CDS             complement(8445..9680)
                     /locus_tag="VGR22_00225"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEV2127026.1"
                     /translation="MPATTDARSAPNPLLVTSEGQTWLVGDEIYLRPPAPADAETVQS
                     WTFTRFPISPDRIRARIDSGERGDDSGKDRHQLLIVRRADERPVGSLIVNTKWFPHFF
                     IDVTTDSLFGPDIDRWKGEALAMAMRLVVDEWHVPIVTVSLREDEREAITRLEAIGAR
                     QCLRLPEVYARDGMRVDSVTYELMHPGWVARLGDPAEVELPRSGTGQPRPVTPPVLPD
                     GDPPANAVRIGPRVYLRPIQESDARVVTYWSLRELDTSWSNGRFTFSPEAFWNEVKEI
                     QSADKPGRVDFAVCLRDSDTLIGFVGVEDVDYQHRFADSASMMINPAYREAGYGSEAK
                     HLLFDYVFNTLDMHVLGSWVMFANTRSAAALRKQGYTEAGRVHWITHRDGAFANMVIF
                     YLRAKDWRAMPRRLEGSAE"
BASE COUNT         1720 a         3285 c         3322 g         1738 t
ORIGIN      
        1 gaccgagggg cgctcctgtc acgttgcccg ttcgccggga gagatggtgt tggtcttgcg
       61 tccaccgggc gcacacgacc gcgggcccgg tggtcggttg tccctgctct ccccgcagat
      121 taccactctt tcgcaccaca atgtcaggga cgctggtgcg tgtgcacatc aggcgcagga
      181 cggaattgga cttcggatga ttgggtcaat gcgattcaaa gcgacgggga ccggccattc
      241 agctgccgcc cgcacggggg ctcaaccgat cagggcagga aataggtcca gtcgcgggtg
      301 ccgaactgct cgtccacgag gcgctgcatc tcggcctcct cctccagagt gagctcgtca
      361 tcgtgcaacc cgaagcgcga acggaagtgc tcgatcaggt gtgcgatgat cgcgtctcgc
      421 tccatctcgg tttgctgccg gagcggaccg acgcgccgat ctgcggagcg gatgcccttg
      481 tcgctgagct tctcctggcc gacgcgcagc acggtcccga gcacccccgt ggtcatctgg
      541 taggccatgg tggtgtgatg cagcaccgcg ccgcctcgac gcgcctgcgc ggcgccgccg
      601 atcttgccgc cctcggacgt gatgtcgttg agtggctggt accacgcgtt gacgccgaga
      661 tcccgcagcg cgtccagcac ccagctatcg aacatctcgt aggactcggc aaagctcagg
      721 ccccgcacaa aggactccgg cacatagacg gaataggtga tcgcgccctc cggctcgacg
      781 aacatcgcgc cgccaccgct gatgcggcga acaacgtcga tctcatgctg ggcggcgacc
      841 tcggcataga cctcgttctg caccgactgg aagcgcccga ggatgatggc cttcctggcc
      901 cacgaccaga accggagggt gggcaggcgc tcgccgcgcc ccacgcgctg ggtcaggacc
      961 tcgtcgagcg cgaggttctc cgccgggagc agcggccgtg gcgggatcac ctgccattcc
     1021 tgatggcgcc agcgctcgcg cagttcctca tgcatctgcg tttgcctcct gcgctcgagt
     1081 gacggcgatc gcgatggcag cgggcgagaa gccgaacagc tccgttccgg gcggaaccgc
     1141 ctccgccacg cgcagcgtgt actcatgcgc cggcagcccc gccggtgcgc cgtcgagcgc
     1201 gcccacaatg gaggtcatcg cctcttccgg gtagagaaag aaatccccgc tcagccgtac
     1261 gtcggtcaat cgcgatgtct cgtcgatgtc gagatctaca acgacgagct tgccacgcgg
     1321 gaccttgaac tctccgtgca tgcatagcgc tccgttcacg cctcggtgcc cagctccatt
     1381 ctcaccgcgt gggcagcatc ctcgccgctc atcatcgcgc cgttgctgga ggcgtcaacc
     1441 gtcgcatccg acgcgagcat caatccctgc acaccggtgg tggcgtccgg caggcggcgg
     1501 tggatgccgg gaacctgccg gtagaggctg aacggcacgg cgactgcctc gacgacgcgg
     1561 aggtcgccga tcccgagcat ggtcgcaacc gactcccgcg cgatgcggcc gttctcctcg
     1621 ccatcgcggg acagtggctc gtcacccagc agcacggcgg ccaggagacg ctcgccgtcc
     1681 ggcgcgtaac tcggctgcac ggcggagagc ggcgcgatct cgttgaccgg ctgatgacca
     1741 gtgccatcga tggccagcat cttcccgatg cccggatcct gccgcgtggc gaggtagacc
     1801 gtgacacatc ccactccctc ggtcgggatc gcatcgatgc cggtcagccg ggcggcggcg
     1861 ggcgggtcgg tggcaacgat gacggcggca gcctcgatcg tctccccggc ggcccggacg
     1921 ccggtggcgc gcgtgccctc gatcgccagc gcgtcaaccg ttgtcgacgt ccgaaccgag
     1981 ccgtcgggta gctcggccgc gatcgccttc ggcaccgcgc cgacgccctc gcgggggagc
     2041 acgccgtcgc cggccaggaa catcttcgtg gtaaagcgga cgacgccggc gctgaacgac
     2101 agcgagcggt cgagcgcgat gccgccccag aagggccgtg cgaagcggtc gatgaactcg
     2161 tcggagaagc cgtgcgcctg cagcgcagca agcgcggact ggtccggcag cacattcgca
     2221 gcctcggctg cagtggtcca cggcgcgagc gccgtctgca ggccccaacg cacgaggcga
     2281 agcttgtcgc cgaacccgaa gaccgggctt gtcaggtcgc gcaggatacc agccgggtgg
     2341 ttcagcgggt tgcgcagcgg cacgagccgc cgtccagtcc agacatgcgc gccagaatcg
     2401 aacggacggg caccgagcac gtcgaggtcg acgttgcgac gcagggcggg ataggcggag
     2461 agcaggatct gaaaccctcg gtcgatgagg aacccgtcgt cggtgcggcg cgtgcgcaca
     2521 cgccccccca cctccggctc ggcctcgagc acgagcacgg aaatcccgtc gcgatgcaaa
     2581 cggcgggcgg cggtcaaccc cgcaagcccc gcaccgacga tgatcacggg gcgtcgctgt
     2641 tcacgcatac gggccgcact cctcgagagg ggctccccgc cttgaaccag cctagttgga
     2701 ggggttgcct ggatagaaca tttgtcctac tatgatcctg tcagcgggtg aacggacacg
     2761 tggcgcttcc ccactaccgc gccgtcccgc accccgattg acactccccc attcccttcc
     2821 ggagcgcgcc tgtggttccc caaccgcggg cgcgctccgc tgtttgggtc aggccatcgc
     2881 ggcgcgaaac ttctcgagca tctcctcatc ccaagcaagg tggttctcga tctcgtcggc
     2941 gagggtgatc tcgccctgct ggtcatggtc aaccttctgg tcccagtcgc gcagggtgag
     3001 gttgaccagt agcgagacaa gctcgctgcg ctggttgttg tactcggtga gcatggcctc
     3061 gggcgtgtcg atgtgctgat cgagggccct gagctccggc tcgtactcga tcgctcgcag
     3121 acgcggcatc cgctcgcgca tcacggcctg cacccgtcgc acctgcacac gctcgcgcgt
     3181 tcccagctcg gcgagcatca ccttgacgtc ctcggggacg tcatccggca cgggattgcc
     3241 gagaatttcg cgcaactccg agggggtctc ggtaagtccc tcgatgaggt cctgaaaact
     3301 gtccagcatg aaggattatc cctgcagtgc cgccggcgtc gtcgtgtcga cggccaccat
     3361 gggcgtgatg ctccacacca cgttgaacgg gtcaacgaag cggagagtgt ccggatcgct
     3421 ctcgagcacg gtgttgccgt agatcagtac gcggccacgg accgtggcga tgtgcttcgg
     3481 cgtggcggtg gtgtggatct ccatgggatc gacaccgtag ggcagtggca agccacgcgg
     3541 gtgccgttcg agattgatcc gcaacggtcc gttctccaag aaggagacat cggcctcccg
     3601 gccatacagg cgcgccatct tgtgatcgta cggttcctca acggcgcgga gttgcccgtt
     3661 gtcctgccga agggcgcgcc cgatcaggcg caggtcgagc acctcgtcgt agtactgctc
     3721 ggcaggccca aggttgtgaa cgggcacgcc gggggccgag gcaagccgca cggccagcga
     3781 ggcgggatcg tcaaggacga gctccggttg gctctcgctg actgcaggcg caccaccgct
     3841 accctcccac accttcggct cgtgcacgcc gatctgcgga atggtgtggt ccatccacgg
     3901 ctggtcacgt ctgatggtgg cgacgatgag gctgacgttg gccttcgcga agctgacgcg
     3961 ccggccgtcg tgcaaaacca ggcgaacatc cgggtagggc tcgccgtagt tagccatcca
     4021 ctggtagatg cgctccacca ccgccaggga gtgtgccgca acggagaaat cgaggtacgg
     4081 atcattgaag ccgaggccgt gcgagttgcg ctcgacgagg atctgcgcgt ccggcacgga
     4141 gttgaccact atgttcgcct cgtcaagtag ggattccggc acctcgagca cgaaccggta
     4201 cgccatcgtc tgtgtctcct ctacgtgctg tcaggtggtc gggggccggt ggcccgtggc
     4261 gatcgcggtg tgcagcgggg tttccacccc aaccacacat gccggccgca tgtgcccacg
     4321 attgtaccaa ctgacatgcc acgaaacgga aacggcatcg ggtgggtgga cggatagccg
     4381 atgcttgtac gttcgctggc gacagccggg aggggtgtgg tagggtgccc gcggttcgtt
     4441 gcgaccaccc atcggcgccg tggctggagg gccgaggggc atcgacgcgg gcaggatacg
     4501 tccgctgcac accactgaga aaggagcaac accgcatcga atcgtgatcg tcatccccgg
     4561 cgatcaccag tacgacgggg gcaaaagcgc tcccgatcga tttcctttga gggaaggaca
     4621 tattacccaa tcatgtcgtt gttcacgctg ccgtcctccg cgacggaggg ttcgacttgt
     4681 gcctcggacc acacacattt cgaccgtttc cgcgctccga tcgcgatgat catcgccgcg
     4741 ctgatgctca tcgggtcgct cggcatggcc accccggctg ccgctatcga gctgctgcca
     4801 ggtcctgggt ccgagcaggc ggtgccgccg gcggaagcct ccagcaccgg ggacgcgatc
     4861 gctggcttcg cgatgcaatt cctcggctat ccctacgtct gggcgggcaa caccccggcc
     4921 ggcttcgact gctccggctt cacgcaatac gtggtgctga gcacggtcgg catcgacatc
     4981 ggccatggca ctgcggggca aacggcctac gggacgccgg tcgcgtgggg tgcgtggcag
     5041 ccgggcgatc tggtctattt cgccaacacc ttcagcgccg gcatcagcca cgccggcatc
     5101 tacatcggtg acgggcagat gatccacgcc gagaatgcga gcacgggcgt gacgatctcg
     5161 agcatctact cggattacta ctccgcgcac tactacggcg cgttccggat cggctagtcc
     5221 gaaccctcgg tgcgctgtag acgacgactt gcctgcgcta gccgtctgaa atggaggacg
     5281 ccgttgtgcg ccaagtgggc ccatctgcgc catgtagacc gcattatgaa gacgggagga
     5341 cattgaacgc gcgcccgacg tctgtcgtgg ctctggcaag cgcgttcaag tcctcccctc
     5401 ctatgtagtc tattgatcgc gagccaggtt ttccgcggct ggtaccattc ttgccacgac
     5461 ctaccccatg agtgccgcga agcacggagc ttcgcggcga gagcggaaag gacggtggac
     5521 gatgtccagc gagaacggca acggaaaggg cgcactgcag atcgaattct gcacgtcctg
     5581 aggctacgaa aagcgcgccg ccagtttggc ggagcatatc ctgcacactc ggccgctcga
     5641 cgtcaccgag ggggtcacgc tgatcccgag cagcggcggg gttttcgagg tcacctacaa
     5701 cggcgaactg atcttctcga agaaggcgga gaaccgattc cccagacgcg aggagatcaa
     5761 ccaggcgatg gatgcggcga cgagctgatt gccgcaccca gcccgagccc gacgccccgg
     5821 atcgcgcgat ccggggcgtc gtcgtgtccg gcggtctcac cttgacatca gctagagggc
     5881 gggtggggtg actgggcccg gctacccctg ctcgccggtg cggggcatgg cgcgccactc
     5941 gtcggcgagg taatcgaaga cgacgaagtt gtcgaacgtg cccttgttgg aatagagcca
     6001 gttgatgcgg ccggcctcgc ggtagccctg cttgcgcagc gccgccgccg agcgggtgtt
     6061 cgggaagtag acccacgagg tgaccatgtg caggtcgagc cggttgaacg cgtagtccag
     6121 cacgaggtgc ttgccctccg agccataccc cttgccacgg aacttcggca ggaagaagct
     6181 ttcggtctcg ccgcggcggt cgtggtagtc cacctcgagg acgccgttac cgccggccag
     6241 ctcgccgctc tcgcgatcgg ccaccgcgaa acgaatccac tccgggaact cctgcttctg
     6301 gaagccctcg gcccaccgcc acttgcccag cttgtgcagc atgtagcgtc cgacgttgaa
     6361 ggcagcctcg gcctccgtct cccgccgcga ccagatcgag aactgctcgg cgtccgcctt
     6421 cgtgaacggg cgaaggtaga cccgctcgcc gaccatgacc gcgttcttcg gcgggtcgct
     6481 caacgagcgc accgatgcgg ggaccggtcg tggctcgcct gagccgatcc gctcgatctc
     6541 gacctcgttg ggatcgccga gcgtgctcac ccactgcgag ttgaggtact ccagcacgat
     6601 cgcgtcgacc cgctggccgt ctcggaagaa gtactcacgg aaagtggcgg tctcgcgcat
     6661 gccgagatcc ttcgcgccgt cgacgaacgc ctgctggctg gcgggcacga tggcatgcag
     6721 attcggccgc tgctgctcgt tgacggacca gccgaggatc atgcgcaggg cctcgagtct
     6781 ccagcgatcg atcttgctgc cgaagagcgg atcgacgaag aggttgagcc agcgaccacc
     6841 gattggagat cgctcgatga cggccatgcc gacgacgcgc tcgtcgctct tgcgccggat
     6901 gccgaggtgc tggcgaccct cggccttgag tatgtcgccc gtgatccact cctcggtgcg
     6961 ctcgggagag agtgggaagc gagagccgcg gaaggacatg gaccacttcg cgtcgtcctt
     7021 gtcgatcggg cggaggtaga tctcctcgcc gacgaggtag gtctgcccct cccaggctag
     7081 tggagtggtg ttgccctcag gggtggtctt ctcggaggtg gatgatgtca cggtcatggt
     7141 ttggtgtctc gtttccatac aatgcgagtt tgcgttatat cgccccgcgg ccggccgggc
     7201 agacccgatc cggccctacg agtcgatgtc cgcatcctga cggggcatgg cgcgccagtc
     7261 ggttgcgagc aggtcgaagg cgacgaagct gacgaagcgc ccgtcgcgga actcgatcca
     7321 gtgctcgcgg ccggcctcgc ggtagccctg cttgcgcagc gccgccgccg agcgcgggtt
     7381 gtcgaacatg acccacgact ggatcgcgtg gacatccaga tcgttgaaca ggtagtcgaa
     7441 catcaggtgc ttggcttcgg tcccataccc cgcctcacgg tatttcgaat tgaggatcat
     7501 gctggccgac tcaccgaacc ggtgcgtgta gttcacgtcg agcaccccga ccatgccgag
     7561 atactcatcg gtctcgcgaa ggcaaaccga gaactcgatc cagtccggcg gggttttctt
     7621 ctgcgtatcg tcgaaccatt tggctactcc ctcccgcccc accgggaacc gtccgttgtc
     7681 ccagctggtg tcgatttccc gcatcgacca gtgggctaca gcctccgcgt cggatttctg
     7741 cggcggtcgc aggtagacgc gcggcccgat ccgcaccgcg ttggtgggcg ggtcgccgtc
     7801 cgggagcacc ggcgctgtca ccgggcgcgg ctgacctgtg ccggtgcgcg ggaagacgac
     7861 gtccgccgga tcacccagcc gcgcgaccca atcccggttc aggtattcca cgacgagctc
     7921 atcgcgccga ccaccgcttg ggcaggagag cttttcgcgg aaccgggttg ttgtgcgcgc
     7981 accgatgccc tccagtgcct cgatgaccag tgtctcatcg gccgggagag cgatcgacac
     8041 cttgggtcgc tgctgctcgt caacgagcat ccggaagacc agcacgagcg cctcggtctt
     8101 cagccgaaat ccctcgtcgc cgagaagcgg gtccagctga gcgcttacgt cgtggtgtgg
     8161 gaaatgccgg ttgtcgaccg tcaccgaccc caccgggcgc tcgttgctgc ggcgcacgac
     8221 gaggtagatt gccttctggt acggattgct gttcttcgcg agatcatcgg cgatccactc
     8281 ccgggcgcgc tcgggcgcca tcgggaatgg agacctcttc caagaactga cgaactcggc
     8341 atcggccggc acgatgggtc gcaggtagat ctcctcgccg acgagccagg tctgcccggc
     8401 ggcacgcgtc agctccgggt tagtctggtc cggggtggat gttgtcattc ggcgctccct
     8461 tccaggcggc ggggcatggc tcgccagtcc ttcgcccgaa ggtagaaaat gaccatgttg
     8521 gcgaacgccc cgtctcggtg ggtgatccag tggacgcgac ccgcctcggt gtagccctgc
     8581 ttgcgcagtg cggccgccga gcgggtgttg gcgaacatca cccacgagcc cagaacatgc
     8641 atgtcgaggg tgttgaagac gtagtcgaag agcaggtgtt tcgcctcgga gccgtagcct
     8701 gcctcacgat aggcggggtt gatcatcatg ctggcggaat cagcgaatct gtgctggtag
     8761 tcaacgtcct cgacgccaac gaagccgatc aatgtgtcac tgtcccgaag gcaaacggcg
     8821 aagtcaacgc gccccggctt gtccgcgctc tggatctcct tcacctcatt ccagaacgcc
     8881 tcggggctga acgtgaagcg cccgttcgac cagctggtgt ccaactcacg caggctccag
     8941 taggttacga cgcgcgcgtc gctctcctga atcggccgta gatagacgcg cggaccgatg
     9001 cgtacggcgt tggcaggcgg gtcgccgtcc ggcagcaccg gcggggtaac tgggcgcggc
     9061 tgcccggtgc cggagcgggg caactcgacc tcggcaggat cgccgagccg cgccacccaa
     9121 ccgggatgca tcagctcgta cgtgacgctg tcgacgcgca tgccgtcccg tgcatacacc
     9181 tccggcagtc gcaggcactg gcgcgcgccg atagcttcga ggcgagtaat ggcctcgcgt
     9241 tcgtcctcac gaagcgaaac cgtgacgatc gggacgtgcc actcgtcgac caccagccgc
     9301 atggccatgg cgagcgcctc ccccttccag cggtcgatgt ccggtccaaa gagggagtcg
     9361 gtggtgacat cgatgaagaa gtgtgggaac catttggtgt tgacgatgag cgaaccgacc
     9421 gggcgctcgt cagcacgacg gacgatcagc aactggtggc ggtccttgcc ggagtcatca
     9481 ccgcgctcgc cactgtcgat acgggcacgg atgcgatctg gtgagatcgg gaagcgggta
     9541 aaggtccacg actgcaccgt ctcggcgtcg gccggagcag gcggccgcag gtagatctcg
     9601 tcgccgacga gccaggtctg gccctcgctg gtgacgagca acggattggg cgcactgcgg
     9661 gcatcggtgg tcgcaggcat ggttcacctc tgagtaatgg ttgcagggac ggcacaacgc
     9721 cacacgggcg cgcagctgcc gggacgaatg atgttgggga gctgggggtg gaagtcaggc
     9781 gatcgcggaa ccgggtggct ccatcacctg gaacaccatt cgaggccgga tggcgtcaga
     9841 tccgcgcacg ccgtggggtg ggaatcagca ttgaacgctc tcctctgtgc cgttgtcggt
     9901 ggggacccga ggtggctccg gcacactcgc cgcgttgtgc gtagagacgt agcggagtat
     9961 acacaacggc cacgagccat gtcaggaaag cagtgactgc ccgagcgtcg gccaccagtc
    10021 aaagggcgga catggagccc gcccatacaa acgacatcct atggc
//
LOCUS       DASYWL010000007         2483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_188022, whole genome shotgun sequence.
ACCESSION   DASYWL010000007 DASYWL010000000
VERSION     DASYWL010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188022"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="VGR22_00230"
     CDS             <1..1071
                     /locus_tag="VGR22_00230"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2127027.1"
                     /translation="AEGRAICGVVSVGDIGVPAWVLQRVGIKVPPAMFARGTVVGWHA
                     DDACRCAHGLMSARRRAVRPVELTMNVHSETLPVTQWRDVIHMEQDVQLAVCGWVPNR
                     PKTILLLSHGYADHPGRHSHVVNPLVADGFAVYGIDHRGHGRSSGVRALITDFDRTAD
                     ELYVLATRAQQRHLGLPTVLVGNSMGGLIALRYALRYPEVLGALVTIAPALIIDQNSS
                     RPEVLIGKLIGTIAPAVPHPYSPTGDCSTTTDPEICRQFAIDHRTWHGHPRIGAAAAM
                     LRAGEDTLDRLGEITTPLLAMHGTEDTLADPCGTELMRKRVGSQDKTVFLLEGMRHEL
                     FNEPDRARVIGVVRSWLAERFG"
     gene            complement(1234..>2483)
                     /locus_tag="VGR22_00235"
     CDS             complement(1234..>2483)
                     /locus_tag="VGR22_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2127028.1"
                     /translation="VVVPVMLVVMWVWQRYAKAAYRVTRRTTSAVNANLAESIAGMRV
                     VQSYVREPMNFRFFQRINFDNFRAHVDAARLSSLLFPLVTVLGTAATAMIVYVGGQLV
                     FDNQMSIGELVLFVALIDRFFVPIRDLSQQYSVLQSAMVAGERIFEVLDVEPEVQDKP
                     DAYELPPIEGRVDYNHVTFGYGNTQILHDINLHVEPGETVAFVGETGAGKSSMVNLLM
                     RFTDVWDGSITIDGHDIRDVTQQSLRAQLGIVLQDTFLFGSTIRENISYARPDATLRE
                     VEAAAREVGAHEFIRRLPEGYDTPVQERGVSLSVGQRQLISFARALIADPKILILDEA
                     TSSIDTQTEKLIQDALKRLLVGRTSFVIAHRLSTIREADTVVVMDQGRIVEMGTHDEL
                     MAKRGAYHGLYTMQWRGSGMAAD"
BASE COUNT          432 a          827 c          783 g          441 t
ORIGIN      
        1 gcagagggcc gggcgatctg cggagtggta agcgtcggtg atatcggcgt gccggcatgg
       61 gttctgcaga gggtcggcat taaggtcccg ccggcaatgt tcgcgcgggg gacagtcgtt
      121 ggctggcacg cagacgatgc ctgccggtgt gcgcatggcc tcatgtctgc aaggagacga
      181 gccgttcgac cggtggaact taccatgaac gtacacagcg aaaccttgcc tgtcacccag
      241 tggcgggatg tcatccacat ggagcaggat gtccagttgg cggtatgcgg ctgggttccg
      301 aaccggccca agacaatcct gctcctgagc cacggctacg ccgaccatcc cggtcgtcac
      361 agccacgtcg taaacccgct cgtcgctgac gggttcgccg tctacggcat cgatcaccgt
      421 gggcacggac ggagcagcgg cgttcgcgca ctgatcacgg atttcgaccg aaccgccgac
      481 gaactctatg tcctcgcgac ccgcgcccag caacgccacc ttggcctgcc gaccgtgctg
      541 gtcggcaact cgatgggcgg tctcatcgcc ctgcgctacg cattgcggta cccagaggtg
      601 ctcggggcgc tcgtcaccat cgcgcccgcg ctcatcatcg atcagaactc ctcccgtccg
      661 gaagttctca tcggcaagct gatcggaaca atcgcccctg cggtcccaca tccatattcg
      721 cctactggtg actgcagtac tactaccgac ccggagattt gccgacagtt cgctattgat
      781 caccgtacct ggcatggtca cccacggatt ggggccgccg ccgcgatgct gcgcgccggg
      841 gaggacacgc tcgaccgact cggggaaatc acgacacccc tgctggcgat gcacggcacg
      901 gaggacacgc tcgcggaccc gtgcggcacc gagctgatgc gcaagcgggt cggcagccag
      961 gacaagacgg tgttcctctt ggaggggatg cgccacgagc tcttcaacga gccggatcgg
     1021 gcccgagtga tcggtgtggt gcggtcgtgg ctcgccgagc gtttcggctg agtggtccga
     1081 ggcaatccgg cggcggcatg ggaagtaggc ccgcttctcc gcgggcgggt tgggctccca
     1141 gcgtcacctg tcgattcctt catccggaca tcgtatccat cgcacgcacc cagatctcga
     1201 aaccggtcca tgtgcctgtt gcgacggatg gggtcaatcc gcggccatgc cgctgccgcg
     1261 ccactgcatg gtgtagaggc cgtggtaggc gccccgcttc gccatcagct cgtcgtgcgt
     1321 gcccatctcg acgatgcgcc cctggtccat caccaccacc gtgtcggcct cgcggatggt
     1381 gctcaaccgg tgggcgatca cgaatgaggt gcgcccgacc agcagccgct tgagcgcatc
     1441 ctggatcagc ttctccgtct gcgtgtcgat gctgctcgtc gcctcgtcga ggatgaggat
     1501 cttcggatcg gcgatcagcg cccgcgcaaa cgagatcaac tgccgctgcc cgactgacag
     1561 actgacacca cgctcctgca ccggcgtgtc atacccctcc ggcagccgtc ggatgaactc
     1621 gtgcgcgccg acctccctgg ccgccgcctc gacctcccgc agcgtcgcat ccggccgggc
     1681 gtagctgatg ttctcgcgga tggtgctgcc gaacaggaag gtatcctgca gcacgatgcc
     1741 gagctgcgcc cgcagcgact gctgggtgac gtcgcgaata tcatgcccgt cgatggtgat
     1801 gctgccgtcc cagacatcgg tgaagcgcat cagcaggttg accatcgagc tcttgccggc
     1861 gccggtctct cccacaaagg cgaccgtctc ccccggctcc acgtgcaggt tgatgtcgtg
     1921 gaggatctgc gtgttgccat acccgaaggt gacgtggttg tagtccaccc ggccctcgat
     1981 cggcggcaac tcataggcgt ccggcttgtc ctgcacctcc ggctcgacgt cgagcacctc
     2041 gaagatgcgt tcgccggcaa ccatcgctga ctgcagcacg ctgtactgct gactgaggtc
     2101 acggatcggc acgaagaagc ggtcgatcag ggcaacgaag agcaccagct cgccgatcga
     2161 catctggttg tcgaacacca gctgcccgcc gacatagacg atcatcgcgg tcgccgccgt
     2221 gcccagcacc gtcacgagcg gaaagagcag ggacgagagc ctggcggcgt cgacgtgggc
     2281 acggaagtta tcgaaattga tgcgctggaa gaaccggaag ttcatcggct cgcgcacgta
     2341 cgactgcacc acccgcatgc cggcgatgct ctccgccagg ttggcgttga ccgcgctcgt
     2401 ggttcgtcgt gtgacccggt aggcggcctt cgcgtagcgc tgccagaccc acatcaccac
     2461 cagcatgact gggacgacga cat
//
LOCUS       DASYWL010000008         4791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_582121, whole genome shotgun sequence.
ACCESSION   DASYWL010000008 DASYWL010000000
VERSION     DASYWL010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582121"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..1362)
                     /gene="queG"
                     /locus_tag="VGR22_00240"
     CDS             complement(160..1362)
                     /gene="queG"
                     /locus_tag="VGR22_00240"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEV2127029.1"
                     /translation="MKLDARVPIRPVPIPEDAGLQRTTAWVKKTAAEHGLEVSAVTSV
                     DPFPEAADAVRSRIDAGHLDGMDWFTHERIDVAEDVRNLHPTARSILSVGLTYWSGPS
                     EKPDDGVPRGRISRYAWGRDYHRVIEKRMKALHADLEDQLGRRIEARFLVDTARVMER
                     AIAARSGLGWYGKHSCIIVPGHGSWILLGELVLDIPLLPDAPLGQDCGRCRICLDQCP
                     TGAIVDAYKVNAPTCISYLTIEERGWIPRELRPKMGDWVYGCDVCQNVCPYTKAAKVT
                     DDADMRPASIENAYPSLPWLLEMTDEEFGAHYFGTPVPRTKRRGLARNAAIALGNIGS
                     DRYIPVLASVLREHDELLVRGHAAWAIGRLAEHDPLTARRALEAARRSEPDAVVCDEI
                     ERAMEVPR"
     gene            complement(1494..2420)
                     /locus_tag="VGR22_00245"
     CDS             complement(1494..2420)
                     /locus_tag="VGR22_00245"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEV2127030.1"
                     /translation="MARIALVGLIDHDEVLHLDHLPVPGTSAVITAVSESPGGTTANT
                     AVALARLGEHVTLRALVGDDAEGAALKTGLEREGVDCTMVTIAGGQPTDRSVILLDTR
                     TGERTILWRKGAMIGRTDKLDVYSLFDHDVVLLDMADEPLRRFLTDLPVHTRPDVLLL
                     GPITYIVDNAEPDATDVAFRFDTITGNAAQFLDLMQVESEGELIETVQHRMPGTNLRA
                     AVVTLAADGCLWITRDEHEYVEGFRVEVRDTIGAGDAHTAGIAYGMARRWSWPETCRF
                     ANAVAAMSTTGAGAQAGLPTIAEVQGLVGNTM"
     gene            complement(2423..4030)
                     /locus_tag="VGR22_00250"
     CDS             complement(2423..4030)
                     /locus_tag="VGR22_00250"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEV2127031.1"
                     /translation="MRARLWTNARITTMDDAKPTAEAILTIDDRIADVGNLDDLQAAA
                     PAGTDTVDLGGAALFPGFIESHNHMIAYGRSLAAIDARPTQLGSIKALVAAIRERASI
                     SPEGGWLIARGYDDNQLAERRHPNRHDLDSVSERHPVLLVNGSGHLAVANTEALRLAG
                     ITATTENPQGGEIVRDAHGQATGLLLETAQRLVQQAMPDPTVDDLVDALQACHDRYVA
                     AGITSSHTAGVNSTDEVTAHQRWAETADNPLRKTIMIGRSMFPAFRDTGMHTGFGDDR
                     LRVGPLKLFSDGSLIGRTAAVSQPFLEDPNPNNLGMEMMPQEELDAIVLDGHTHGYQV
                     AIHAIGDRAIEMCLDAFKRAQEAHPRDDARHRIEHCGILRPDLIDRLAREQVIPVSQP
                     VFIREYGEGFIRHLGRERTALTYPFRSLLGAGIPVVFSSDCPVSAHEPLKSIQASVEE
                     RTNAGTEYEPQEAITPGEAIRAYTIHGARAGFAEDRVGTICPGMLADFTILARHPEDV
                     APAEIDQIPVVGTVIGGELAYQRPMEA"
     gene            4161..4631
                     /locus_tag="VGR22_00255"
     CDS             4161..4631
                     /locus_tag="VGR22_00255"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV2127032.1"
                     /translation="MPRRRKTTSEGTQPSPLGQWLRSIRGEQGMSQRSLADRAGISRS
                     YLCDIERGRGAQPSIETIDRLAVALGYSRDDLLRAGGMIEGGPGDRPTDDERRLLAVF
                     RGLDDDGKSLVMRFARFVHADEHSWIQAGLGVGDDAQPASSTAHQSGPSLFDLE"
BASE COUNT          760 a         1489 c         1689 g          853 t
ORIGIN      
        1 ggcgtagccg ggcctcggct caggatctca aacgcgagga gctccacgtg cgaaccttga
       61 ggacaagcgt gaaagtgatg ctttaacaag gctgcggcgc aggtgcctcg ccggttcagc
      121 atgacatcga tgttaccgtg gtccttttcg gctccattct catcgcggaa cctccattgc
      181 ccgctcaatc tcatcgcaga caacagcgtc aggctccgat cgtcgtgctg cctccaaggc
      241 ccgtcgggcg gttagcgggt cgtgctcggc gaggcggccg atcgcccagg cggcatggcc
      301 gcgcaccagc agctcgtcgt gttcccgcag cacgctcgcg agcactggga tgtagcggtc
      361 gctcccgatg ttgccgagcg cgatggcggc gttgcgtgcc agcccgcggc gctttgttcg
      421 cgggaccggc gtgccgaagt agtgggcgcc gaactcttcg tcggtcatct ccagcagcca
      481 agggagcgag gggtaggcgt tttcgatgct cgctgggcgc atgtcggcgt cgtcggtgac
      541 cttcgccgcc ttcgtgtagg ggcagacatt ctggcagaca tcacagccgt agacccagtc
      601 tcccatcttc gggcgcaact cgcgcgggat ccagccacgc tcctcgatgg tgaggtaaga
      661 gatgcaggtg ggcgcgttga ccttataggc gtcgacgatc gcgccggtcg ggcactggtc
      721 gaggcaaatc cggcaacgac cgcaatcctg cccaagcggg gcgtccggca gcagcgggat
      781 gtcgagcacg agctcgccga gcaggatcca cgacccatgg ccagggacga tgatgcatga
      841 gtgtttgccg taccagccga gcccggatct cgccgcaatg gcacgctcca tgacccgcgc
      901 cgtgtcgacg aggaatcgcg cctcgatccg gcgcccgagc tggtcctcca gatccgcgtg
      961 gagcgctttc atgcgcttct cgatgacgcg gtggtagtcg cgaccccagg catatcggga
     1021 gatacgcccg cgcgggacgc cgtcgtccgg cttctcggat ggtcccgacc agtaggtaag
     1081 cccgaccgag agaattgacc gcgcggttgg gtggaggttg cgaacgtcct cggcgacgtc
     1141 gatgcgctca tgggtgaacc agtccatgcc gtcaaggtgt ccggcatcga tgcgggaccg
     1201 cactgcgtcg gcagcctccg ggaacgggtc gacgctggtg accgcgctta cctccaggcc
     1261 gtgctccgca gcggtcttct tgacccaggc ggtggtccgc tgcagccctg cgtcttcagg
     1321 aatgggtacg ggtcgaatgg ggacgcgcgc atcaagcttc ataccggcca gtatagacgg
     1381 ctggcgatgg tccctcggtg gcgatgatcg tgataccggt agatccggct cgccgtgggc
     1441 gggcctgtgc agcgaccggg aatcggtgac gacctgccca cacaattccg gcgctacatg
     1501 gtgttgccca ccagtccctg cacctcggcg atggtgggca agcctgcctg ggctccggca
     1561 cccgtggtgc tcatggcggc gacggcgttt gcgaagcggc atgtctccgg ccatgaccat
     1621 cgtcgcgcca tgccgtaggc gatcccggcc gtatgggcat cgcccgctcc gatggtgtcg
     1681 cgaacctcga cccggaaccc ctcgacgtac tcgtgctcgt cgcgcgtgat ccacaggcag
     1741 ccgtccgctg ccagcgtcac gaccgctgcg cggaggttgg tgcccggcat ccggtgctgc
     1801 accgtctcga tcagctcgcc ctcggattcg acctgcatga ggtcaaggaa ttgcgcggcg
     1861 ttgccggtaa tcgtgtcgaa gcgaaaggcg acatcggtgg cgtccggctc ggcgttgtcg
     1921 acgatgtacg tgatcggccc gagcaggagc acatccggcc gggtgtggac gggaagatcg
     1981 gtcaggaagc ggcggagtgg ctcgtcggcc atgtcgagca gcacgacgtc gtggtcaaac
     2041 agggagtaga cgtcgagctt gtcagtccgg ccgatcattg cgcccttgcg ccagaggata
     2101 gtgcgctcgc cggtgcgggt gtcgagcaga atcaccgagc ggtcggtcgg ctgccctccg
     2161 gcgatcgtca ccatcgtaca gtcgacaccc tcacgctcga gccccgtctt cagcgcggcg
     2221 ccctcagcgt cgtcgccgac gagtgcacgg agcgtcacgt gctcacccag ccgggcgagg
     2281 gcgacggcgg tgttggcggt ggtgccaccg ggcgactcgc tgaccgcagt gatcaccgcc
     2341 gacgtgccag gcactggtag gtggtcgagg tggagcacct cgtcgtggtc gatcaggccg
     2401 acgagcgcga ttcgggccat ccctacgcct ccatcggcct ctggtaggcg agctcgccac
     2461 cgattaccgt cccgactacg gggatctggt cgatctctgc cggcgcgacg tcctccgggt
     2521 gccgggcgag gatcgtgaag tcggccagca tgccggggca gattgtcccg acccggtcct
     2581 cagcgaagcc ggcgcgtgcg ccgtggatgg tgtaggcgcg gatcgcctca ccgggggtga
     2641 ttgcctcctg cggctcgtac tcggtgccgg cattcgtgcg ttcctcgacc gacgcctgga
     2701 tcgacttcag tggctcatgg gccgagaccg ggcagtcgga cgagaagacg accgggatac
     2761 cagcgcccag caatgagcgg aaggggtagg tgagggcggt gcgctcgcga ccgaggtggc
     2821 ggatgaagcc ctcgccgtac tcccggatga agaccggctg ggagaccgga atcacctgtt
     2881 cgcgcgccag tcggtcgatg aggtcgggtc gcaggatgcc gcagtgctcg atgcggtgtc
     2941 gggcgtcatc tcgcggatgg gcctcctgcg cccgcttgaa cgcatctagg cacatctcga
     3001 tcgcgcggtc accgatcgcg tggatagcga cctgatatcc atgcgtgtgg ccgtcgagca
     3061 cgatcgcgtc cagttcctcc tgcggcatca tttccatgcc gaggttgttc gggttgggat
     3121 cctcaaggaa cggctggctg acagcggccg tgcggccgat aagggagccg tctgagaaga
     3181 gcttgagggg gccgacgcgc aggcggtcat cgccgaagcc cgtatgcatg ccggtgtccc
     3241 ggaaggcggg gaacatggac cgcccgatca tgatcgtttt gcgcagcggg ttgtcggccg
     3301 tttccgccca gcgctggtgt gcggttacct cgtcggtact gttcaccccc gccgtgtgac
     3361 tggaggtgat accggcggca acgtagcggt cgtggcaggc ctgcagcgcg tcgacgaggt
     3421 catcgacggt tgggtccggc attgcctgtt ggaccaatcg ctgcgccgtc tcgagcagga
     3481 ggccggtcgc ctggccgtgg gcatcacgta caatctcgcc gccctgcggg ttctcggtcg
     3541 tggcggtgat ccccgccagg cgcagcgcct cggtgttggc gaccgcaaga tggccggagc
     3601 cgttgacgag gaggaccgga tggcgctccg agacgctgtc cagatcgtgc cggttgggat
     3661 ggcgcctctc ggcgagttgg ttgtcgtcgt acccgcgtgc gatgagccag cctccctccg
     3721 ggctgattga ggcgcgctcg cggatcgctg ccaccagcgc tttgatcgag ccaagctggg
     3781 tcgggcgcgc gtcgatcgcc gccagcgaac ggccatacgc gatcatgtgg ttgtgggact
     3841 cgatgaagcc ggggaagagc gcggcacccc ccaggtccac ggtgtcggtc ccggccggtg
     3901 cagctgcctg cagatcgtcg agattaccga cgtcggcgat gcggtcgtcg atcgtcagga
     3961 tcgcctcggc ggtcggcttg gcatcgtcca tcgtggtgat gcgggcgttg gtccagagtc
     4021 gtgcgcgcat ggggtgagac tcccggggtg tgcagaatat gctacggcca tggtgcgggg
     4081 gttgagcgag gagcgcaagt accggaagat agaggagcgt ttcgcgaggg ccgacacatg
     4141 cagacggcga ggacacaggg atgccacgga ggcgaaagac gacatcggaa ggcacccagc
     4201 cgtcgccgct cggccagtgg ctgcggtcga tccgcggtga gcagggaatg agccagcgga
     4261 gtctcgccga tcgggcgggg atcagccggt cgtatctctg cgacatcgag cgggggcgag
     4321 gagcgcagcc gagtatcgag acgatcgacc ggctggcggt agcgctgggc tactcgcggg
     4381 atgacctcct tcgcgctggc gggatgatcg aggggggtcc cggcgatcgc ccgactgacg
     4441 acgagcgccg gctgctggcg gtcttccgtg gcctggacga tgatgggaag tcattggtga
     4501 tgcggtttgc gcgcttcgtg cacgccgatg agcatagctg gatccaggca ggacttggcg
     4561 tcggcgacga tgcccagccg gcgtcgtcca ccgcccatca atcgggtccg tccctgttcg
     4621 acctcgagta gaggctccat cgaaacgctc ccaacgtgtc ttttcgagcg catgtgagcc
     4681 tgcagtcaag aaatctcctg gctgcccgaa gttgatgccc cttacagaag cgatttctcg
     4741 acttcgcggc ttcgccgcta cgctcgataa tgacacattg agtgggcgaa a
//
LOCUS       DASYWL010000009         8141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_188033, whole genome shotgun sequence.
ACCESSION   DASYWL010000009 DASYWL010000000
VERSION     DASYWL010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8141
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188033"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(357..983)
                     /locus_tag="VGR22_00260"
     CDS             complement(357..983)
                     /locus_tag="VGR22_00260"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsnF/AvaK domain-containing protein"
                     /protein_id="HEV2127033.1"
                     /translation="MENPQFVENARVVDREGAEIGTLRAIEEGEAGSIVIARTPDGRE
                     VGIPYEQIDLRASTDEEVVADLLVASLEIPSEGVAEDHYATLTLSEEDLDVSTRWAEK
                     GRVIVRKRVETVPQEKTIELAQEGVEVERVRINDVVDELPQVRTEGETTIIPVVEEVL
                     IVEKRYRLVEEVRVTSTRSSQPHTIREDLRREVVEFEEVPPEEGTAER"
     gene            1155..1556
                     /locus_tag="VGR22_00265"
     CDS             1155..1556
                     /locus_tag="VGR22_00265"
                     /inference="COORDINATES: protein motif:HMM:NF021458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2171 domain-containing protein"
                     /protein_id="HEV2127034.1"
                     /translation="MARNSTPAVPEKGMQVYGSDAGRLGDIRRIEGDFLVVDQGILRD
                     DLYIPRSVVDRIDQQGRVVLTLSKDEVKSRGWTDPALGGAITVSGQPAEHRPDALNQV
                     STTATPGMDDVINVDTIEFDDRPDMDTNRTE"
     gene            1567..3648
                     /locus_tag="VGR22_00270"
     CDS             1567..3648
                     /locus_tag="VGR22_00270"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEV2127035.1"
                     /translation="MSNRLANEPSPYLQQHKDNPVDWWPWSDDALEEARQQDKPLFVS
                     IGYSACHWCHVMAHESFEHPELAAFMNEHFLNVKVDREERPDVDTIYMNAVQAMVGHG
                     GWPLNVFATADGVPFFGGTYWPPVDARGMPGFVRVLETIATAWQRDRESLLENARRVT
                     GYLESISQQRPNYEAVRPSLADEALETLGNSFDSHWGGFGSAPKFPQVPTLDFLARHL
                     QRTGSDEARRMLTTTLDRMAAGGIHDHLAGGFARYSVDAQWVVPHFEKMLYDNAQLIR
                     SYLEAWKATGTPRYAEVVRQTGNWLLTEMQLPGGGLASALDADSEGVEGKFYVWSDSE
                     IHELLDGDVAEVVHRRFGIAPAGNFEGNNILTLAESVEDIATSLGKPVSEVQKLLDEA
                     TARLRERRSSRVRPGQDDKVVTAWNGLAVTGLALVGAALRIPRFVDAAEEAARFMLTS
                     VRNDDGTLWRTWKDGERRGNGVLEDYIFLAEGLIHLHQATGKIRYLDEADAIFGKAVE
                     LFNREGGADFYDTPAEGTGLAVRPHTLQDNATPAGNSVAADVLLTLSEVTSRSEMAER
                     AEEIVSGVADLLTEHPGAFGRYLAVAERLYSTPYTLVIAGDPTSDIHRELTQLALAHP
                     QPSLVVTHATPKLDERTLHRYPVLQERAAVHGVSTGWLCRQGACMLPATTVDELQERL
                     RETDRSLTAEA"
     gene            complement(3728..4276)
                     /locus_tag="VGR22_00275"
     CDS             complement(3728..4276)
                     /locus_tag="VGR22_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127036.1"
                     /translation="MPDKRRDEEQLPPIPDGGLAESMPEWLRRPPAWRTLRDEEVVQA
                     EPPETDEIPEADDSPIDPRTFLTDDDLPDWLRRLGSVSSPQPESGADERVAVAADERT
                     EPEPSAAVSRPVASAHFVPRSPAVTHAEGDRKSLSPPARAPSMRRQTVIHRAAWWQGP
                     QLEILLAVALLMALIVIVILVI"
     gene            complement(4289..4768)
                     /gene="dtd"
                     /locus_tag="VGR22_00280"
     CDS             complement(4289..4768)
                     /gene="dtd"
                     /locus_tag="VGR22_00280"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEV2127037.1"
                     /translation="MRILIQRVTQASVTVGGVITGAIGSGLALLIGIGEDDTPDLVEP
                     MCEKVVSLRIFDDAGGRMNRSALDLLDEGEQPGMLVVSQFTLYGDLRKGRRPSYSHAA
                     PPEAASPMVDACAELLRKTGFTVGTGVFGAEMQVALVNDGPVTLWLDSDDMKRPRRA"
     gene            complement(4756..5280)
                     /gene="ispF"
                     /locus_tag="VGR22_00285"
     CDS             complement(4756..5280)
                     /gene="ispF"
                     /locus_tag="VGR22_00285"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEV2127038.1"
                     /translation="MYRAEVPRTGIGYDVHRFAEGRRCVLGGVDIPFERGLEGHSDAD
                     VVLHALADALLGAAALGDIGRHFPPSDPQWAGMDSGDIVRRSVVLLSKIGMEPIHADI
                     TVIAEVPRINPHLSAMQAAIGAVVGLPPETISVKATTSERMGFVGREEGIAAIAVATI
                     ARVPGEGERLKCGS"
     gene            complement(5267..5980)
                     /gene="ispD"
                     /locus_tag="VGR22_00290"
     CDS             complement(5267..5980)
                     /gene="ispD"
                     /locus_tag="VGR22_00290"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEV2127039.1"
                     /translation="MTDRFAGVIIVAAGRGERFGDSGKVLAYAGGRPLLARSLDAAIA
                     ASSTAEIVVVCGGHTRERIAALIESLDTPLPVCVCAGGAERQDSVRFGIHALSDSIEV
                     AVIHDAARPLATSAMFDDVAEAAREQGAAIVAVPVTDTIKEVEGDRILRTIPRQMLRA
                     AQTPQAFRLDTLWDAMERTTGSNVTFTDEATRLESLGVPVVVRPGTTTNIKVTVPEDL
                     VLVEALLQSQCRGTRDVPS"
     gene            complement(5977..6852)
                     /locus_tag="VGR22_00295"
     CDS             complement(5977..6852)
                     /locus_tag="VGR22_00295"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEV2127040.1"
                     /translation="MTAKDTNRANRPSEGSFWPVDLHAHTTASDGTLAPDELVDFAVQ
                     RGVEILGVTDHDTVDGIEAASRRASERRVTLMPGVELSTTERGSEVHVLGYGVDLRDA
                     SLRKRLADLAASRVRRISAMIEKLCEAGYAIDGDAVLAQADEGSIGRPHVARALIEIG
                     VVASVDEAFDRFLKPGRPGWVPREPFTPEQAVQLLRDHGVLPVLAHPLSTRDVPGVLR
                     RLIPVGLRGMEVWYGEYDPGEREALRRTAEEHNLLATGGSDYHGPRFRAGRELGSVPV
                     PREVFDRLVDAGVRV"
     gene            complement(6869..7120)
                     /locus_tag="VGR22_00300"
     CDS             complement(6869..7120)
                     /locus_tag="VGR22_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003181955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HEV2127041.1"
                     /translation="MKVSARSRPSAVAGAIAGVVRESGRAEVQAIGAGATNQAVKAAA
                     IARGYLLETGIDVVCVPAFIDVTIENEDRTAIRLVIEPR"
BASE COUNT         1476 a         2750 c         2491 g         1424 t
ORIGIN      
        1 caagacggcc gagacgcaca ccgagcgggt gcgcgacacg gtgcgccgcg aggaagtcga
       61 cgtcgagggt gaggagatcg acaccgacgt tgacgtcaac cgcgaccgag gctgatctca
      121 tcaccgtaac cggccggcac atagctgacg aggtgggacc cacagggccc tgcctcgtcg
      181 cattgggctg cagttgcgca cggcgatcgg acgacaaccc tcaccgctgg gttcgtgcga
      241 cgacacgtca ggtcaaggca gttcaaccca cgctggggac gccgtcgcag ggcgccggtg
      301 cctcaacgcg agcgccgagg cagcggcgct gaagcgaatc ggtttccagc caacgctcaa
      361 cgctcggctg tgccctcttc aggaggcact tcctcgaact ccacgacctc acgccgcaag
      421 tcctctcgaa tcgtgtgcgg ttggctcgat cgcgtggacg tgactcgcac ctcctccacc
      481 agccgatagc gcttctccac tatcagcacc tcctcgacga ccgggatgat cgtcgtctcg
      541 ccctcggtgc gaacctgcgg cagctcgtcg accacgtcat tgatgcgcac gcgctcgacc
      601 tcaacgcctt cctgcgccag ttcgatcgtc ttctcctggg gaacggtctc tacgcgcttg
      661 cggacgataa cgcgcccttt ctcagcccag cgcgtggaga cgtccagatc ctcctcgctg
      721 agcgtcaagg ttgcgtaatg atcttcggcc actccctcgc tggggatttc cagtgaggcc
      781 acaagcaagt cggccaccac ctcctcgtct gtggaggccc tcaggtcgat ctgttcgtat
      841 ggaatgccga cctcacgccc gtccggtgtt cgggcgatga cgatcgatcc tgcttcccct
      901 tcctcgattg cgcgcagcgt gccaatctcg gcaccctcac ggtcgacgac tctggcattc
      961 tcgacaaatt gcgggttttc cattgtgatg tcccttttcc ctttctgcgt ctcgacagca
     1021 ggtactgccc ttccggtgca atccacgtgc cagaatcacc gcccctcggc agcgtggcac
     1081 ggccagtgcg cggaacggga tgaaaagtcg gcgccatcgc cgtcttggcg cccgaacaat
     1141 cggaggcatt tgccatggca cggaattcca ctcccgccgt ccctgagaag gggatgcagg
     1201 tctacggctc ggacgcagga cgtctcgggg acatcaggcg catcgagggt gacttcctcg
     1261 tcgtcgatca gggaatcctg cgagacgatc tctatatccc tcgatcagtg gtcgaccgga
     1321 ttgatcagca gggccgggtg gttctcacct tgtcgaagga cgaggtcaag tccaggggct
     1381 ggaccgaccc ggcgctgggt ggagccatca ccgttagcgg ccagcctgct gagcatcgac
     1441 ccgatgcgct caatcaggtg agcaccaccg cgacaccggg gatggatgac gtgatcaacg
     1501 tcgacaccat cgagttcgac gaccgcccgg acatggacac caaccgaacc gagtgaaagc
     1561 acgatcatgt caaatcgact ggcgaacgag ccgagcccct atctgcagca gcacaaggac
     1621 aacccggtcg attggtggcc gtggtctgat gacgcactcg aggaggcccg acagcaggac
     1681 aagccgctct tcgtgagtat cggctactct gcgtgccact ggtgtcatgt tatggcacac
     1741 gagtcgttcg agcatcccga gctcgcagcg ttcatgaacg agcacttcct caatgtcaag
     1801 gtcgaccgtg aggaacggcc tgacgtcgat acgatctaca tgaacgcagt tcaggcgatg
     1861 gtcggccacg gtgggtggcc actcaacgtc ttcgccacgg cggacggtgt ccccttcttc
     1921 ggcggcacct actggccgcc ggtcgacgct cgtggcatgc ccggcttcgt gcgcgtgctg
     1981 gagacgattg ccaccgcttg gcaacgggac cgggagtcgc tattggagaa cgcacgtcgc
     2041 gtgaccggtt acctggagtc gatcagccag caacggccca attacgaagc ggtcaggccg
     2101 agtctcgccg atgaggcgct tgagaccctg ggcaactcct tcgacagcca ctggggcgga
     2161 ttcggcagcg cgccgaagtt cccgcaggtg ccgacactcg acttcctggc ccggcacctg
     2221 cagcgcacgg gctccgacga agcccggcga atgctgacca cgacgctcga ccgcatggcg
     2281 gccggaggca tccacgacca tctggccggc ggctttgctc gctactcggt ggatgcgcag
     2341 tgggtagtcc cccacttcga gaagatgctc tacgacaacg cccagctgat acgcagctat
     2401 ctcgaagcgt ggaaggcgac gggcactcct cgctatgcag aggtggttcg acaaaccggc
     2461 aactggctgt tgacggagat gcagctgccc ggcggggggc tcgcctccgc gcttgacgcc
     2521 gacagcgagg gcgtcgaggg caagttctac gtctggtcgg atagcgagat ccacgagctt
     2581 ctggatggcg atgtcgctga agtggtgcac cgccgcttcg gtatcgctcc ggctggcaat
     2641 ttcgagggaa acaatattct cacgctcgcc gagagcgtcg aggatatcgc cacatcgctc
     2701 ggcaagccag tttccgaggt acagaagctg ctggacgaag ccaccgccag gcttcgcgag
     2761 cggcgatcga gccgtgtgcg tcccggccag gacgacaagg tggtcacggc gtggaacggt
     2821 ctcgcggtca ccgggctcgc gctcgtcggc gcagcactgc ggattccccg cttcgtcgat
     2881 gccgctgaag aagccgcccg tttcatgctg acgtctgttc gtaacgatga cggcacgctc
     2941 tggcgcacct ggaaggacgg cgagcgacgt ggcaacggtg tgctggagga ctatatcttc
     3001 ctcgccgagg gactcatcca cctgcaccag gcgaccggca agatccgcta cctcgatgag
     3061 gcggacgcca tcttcggcaa ggctgtggag cttttcaacc gcgagggtgg tgcggacttc
     3121 tacgacactc cggcggaggg caccggactg gctgttcgac cacatacact gcaggacaac
     3181 gccactccgg ccggcaactc cgtcgcagca gacgtgctgc tgacactcag tgaggtcacg
     3241 agccggagcg agatggctga gcgcgctgag gaaatcgtct caggcgtcgc cgacctgctg
     3301 accgagcatc caggcgcgtt cggacgctat cttgcggttg ccgagcggct ctatagcacg
     3361 ccctacacac tcgtgatcgc cggagaccct acatcggaca tccaccgcga gttaacccag
     3421 ctcgcgcttg cccacccgca gccatcgctc gtcgtcacac acgctacacc gaagctggac
     3481 gagcgtaccc tccaccgcta cccggtgctg caggaacgcg cagcggtaca tggcgtcagc
     3541 accggctggc tttgtcgtca gggagcctgc atgctgccgg cgacaacggt cgacgagctt
     3601 caggagcgac tccgcgaaac ggaccgctcc ctgaccgccg aggcctgaga cccaccaaga
     3661 aggctggtat gccctgaccc gggagcagca cggcgatggc aatagggctt gctgcactcg
     3721 gtggcagtca gatcaccagg atcacaatca cgatgagtgc catgagcagt gcaacggcca
     3781 acagaatttc cagctgtggt ccctgccacc atgccgctcg gtggatcacg gtctgacgcc
     3841 gcatcgaagg cgcgcgcgcg ggcggcgaca gggacttccg gtctccttca gcgtgagtga
     3901 ccgctggcga gcgcggcaca aagtgcgctg atgcgaccgg tcgggaaact gccgcgctcg
     3961 gctccggttc tgtccgctca tcggccgcaa cagcgactcg ctcatcggct ccggattcgg
     4021 gttgcggtga cgacacagac cccaaccttc gtaaccaatc cggcaggtca tcatccgtca
     4081 ggaacgtacg tggatcgatc ggcgagtcgt cggcttccgg gatttcgtcg gtctccggcg
     4141 gctcggcttg cacaacctcc tcgtcccgca atgtccgcca ggcaggagga cgtcgaagcc
     4201 attccggcat cgactcggca agcccgccat cggggatcgg cggcagttgc tcctcgtcac
     4261 ggcgcttgtc gggcatatcg gccgcctcct acgctcgccg cgggcgcttc atgtcgtcgg
     4321 agtcgagcca cagtgtcacc ggcccgtcgt tcacgagtgc cacctgcatc tccgccccaa
     4381 agacgccggt ccccaccgtg aacccggtct tccggagcaa ctccgcacag gcgtcaacca
     4441 tgggcgacgc ggcctcgggc ggcgcggcgt ggctgtagct cggacggcgg ccctttcgca
     4501 ggtcgccgta gagcgtgaat tgcgacacca cgagcatgcc cggctgctcg ccctcatcga
     4561 ggagatcgag cgccgagcga ttcatgcggc cgccggcgtc gtcgaagatg cggaggctga
     4621 ccaccttctc gcacatcggc tcgaccaggt ccggggtgtc gtcctcgccg atgccgatca
     4681 gcaacgccag gcctgacccg atcgcacccg tgatcacgcc accgacggtc acgctcgcct
     4741 gggtcacccg ctggatcagg atccgcactt cagcctctcc ccttcaccgg gcacacgcgc
     4801 gatcgttgcg acagcgattg ccgctatgcc ctcctcacgg ccgacaaatc ccatccgctc
     4861 gctggtcgtt gccttgacgc tgatcgtctc gggcggcaga ccgaccactg caccgatcgc
     4921 cgcctgcatc gcggagaggt gcggattgat tcgcggcacc tcggcaatca ccgtgatgtc
     4981 agcgtggatc ggctccatgc cgatcttcga gagcagtacg accgaccgcc gcacgatatc
     5041 accactgtcc atgccggccc actgcggatc gctgggcggg aagtgccgac cgatatctcc
     5101 cagcgctgcc gcacccagca gggcatccgc cagcgcgtgg agcaccacat cggcgtccga
     5161 gtggccctcc agtccgcgct cgaacgggat gtcgacaccg ccgagcacgc atcgccgccc
     5221 ctcggcgaaa cggtggacgt cgtaaccgat accggtccgt ggaacctcag ctcggtacat
     5281 cgcgagttcc ccggcactgg gactgaagca acgcctcgac cagcaccagg tcctccggca
     5341 ccgtgacctt gatattcgtg gttgtccccg gtcgaaccac gactggcaca ccgagcgatt
     5401 cgagccgcgt tgcctcgtcc gtgaacgtca cgttggagcc ggtggtcctt tccatggcat
     5461 cccacagcgt gtcgagccgg aacgcctggg gtgtctgggc cgcgcgcagc atttgccgcg
     5521 gaatcgttcg gaggatgcgg tcgccctcga cctccttgat cgtgtccgtg accggcacgg
     5581 ccacgatggc cgcgccttgc tcacgagccg cctccgcgac gtcatcgaac atcgccgatg
     5641 tcgccaacgg ccgtgccgca tcgtggatca cggcgacctc aatgctatct gaaagcgcgt
     5701 ggatgccgaa acgcaccgag tcctgccgct cagcaccgcc ggcacagacg cacaccggca
     5761 gcggggtgtc aagggactcg atcaacgcag cgatacgctc gcgcgtgtgg ccaccgcaga
     5821 cgacgacgat ctccgccgtt gaggatgccg caatcgcggc atccagactc cgcgccagca
     5881 agggacgccc tccggcatag gcgagcacct tgccgctgtc accgaagcgc tccccgcgcc
     5941 cggcggcaac gatgataacg cccgcaaaac ggtccgtcac acccgcactc cggcgtccac
     6001 caaacgatcg aacacctcac gtgggaccgg cacggatccg agctcacgac cggcccggaa
     6061 acgcgggcca tggtaatccg agcctcccgt ggccagcagg ttgtgctctt ccgcggtgcg
     6121 ccgcaacgcc tcccgctccc cggggtcata ctcgccgtac cagacctcca tgccgcgcaa
     6181 tccgactggt atcagccgcc tcaggacgcc ggggacatcc ctggtcgaca gcgggtgtgc
     6241 gagtacaggc aggacaccgt gatctcgcag gagctgtacc gcctgctcag gcgtgaacgg
     6301 ctcgcgcggg acccatcccg gccgacccgg cttgaggaac cggtcgaacg cctcgtcgac
     6361 ggaggccacc accccgatct cgataagcgc gcgggcgacg tggggccgac cgatggatcc
     6421 ctcatccgct tgggcgagga cggcgtcgcc gtcgatggcg taccccgctt cgcacagctt
     6481 ctcgatcatg gcgctgatac gacgcacgcg cgacgccgcg aggtcggcca ggcgcttccg
     6541 cagcgaagcg tctcgcaggt cgacgccata gccgagaacg tgcacctcgg agccccgctc
     6601 ggtggtcgag agctcgacgc cgggcatgag cgtgacacgc cgctcggacg ctcggcgaga
     6661 cgccgcttca atgccatcga ctgtgtcatg atccgtcacg ccgaggatct cgacgccgcg
     6721 ctggacggcg aagtcgacga gctcatcggg cgcgagtgtg ccgtccgaag cggtggtgtg
     6781 ggcatgcagg tccaccggcc agaacgaccc ttcgctcggc cggttggctc gattggtgtc
     6841 tttagcggtc atggaatcct gagtgtgtct agcgcggctc gatcacgagg cgaatcgccg
     6901 tgcgatcctc gttttcgatg gtgacgtcga tgaaagccgg gacgcagacg acgtcgatgc
     6961 cggtttcgag caggtagccc cgcgcgatag ccgccgcctt gacggcctga ttggtcgcgc
     7021 cagcgccgat ggcctgaacc tcggcccgac cggactcgcg gaccacgccc gcgatcgcac
     7081 cggcaaccgc actcggacgc gaacgtgccg agactttcag aacctcgtcg ccgggctcag
     7141 gaacataggt cgacgcgagg gcttcagccg agccgtagcc attttccgac acgggcgcga
     7201 ggtcttcgtc tgccgcggtt gacgcctcgt gctcctcggg ggcaaactcg atttcatcga
     7261 gctgtgttgc gtggctgtcc gatccgccgg ccggctcggc atccacctcc tcttctccgt
     7321 cgggcggaaa ccgcgtgcct tcaggatcga tcttgatatc acgcagatca agcttgctga
     7381 aaaaacgatt catgaaagag gccctcccca tagtgtcccc catgcgtgcc catagccagc
     7441 ggacgaatcc gtgccggagc gccgccttcc cccgcgttga gcgaaccggt caactccgat
     7501 ccaactcgct ggacggcata ccggcagaga tggtgtcgac gaatgcgtca acgatctctc
     7561 cgagatatga gtttacgatg ttatcccgat gatggcacgt tcgggcgtcc tgcaccagcc
     7621 cggagatgcg aaaacgggtg gactggaagt ccatctagtg cgccgtacac tgcataccac
     7681 cggatacctc tagcctgtcg ttacggcgca tctcacgtgc gatatgtgca ttgtgtatca
     7741 atcgctattc tgacggctat tctgacatcg gccctcaagc gcacctctcg acctggagcg
     7801 cagcgaaaaa caatcgttga cgattcactc tatctatacg cacgccccgt ttggaagtgg
     7861 gattttcggg atttcgggat ttgccccgaa acggcgggca gaatccggtc ccgattttcg
     7921 gctctatccc gtttttttcg ggacatgcct caggtcggca cgtcgacggt tagctgccct
     7981 ccagcggcaa cgctcagagc aatatcccga atcggctcta ctttcgaggt ccagcggctt
     8041 ttcgcagtgc acacagagtc tcagccgctc aatgtctcca tcgtgaaccg ggtctctcga
     8101 gctgacggcg ttcatccaga gtcgcacgta caacaggctg a
//
LOCUS       DASYWL010000010         8472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_56548, whole genome shotgun sequence.
ACCESSION   DASYWL010000010 DASYWL010000000
VERSION     DASYWL010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8472
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56548"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VGR22_00305"
     CDS             complement(<1..389)
                     /locus_tag="VGR22_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127042.1"
                     /translation="MAELVYLATHGPDDPTKACMLIHLAVNDAAEAGIEAEVNLAGDA
                     VVLIHDAIIDAVLPIGFPPLKDLLAKAREKDVHVFIMRWLRACPWGQRRRSRRQERLL
                     LDANRVRTMLRRSHQRPSVLACGDPGGA"
     gene            complement(628..2427)
                     /gene="thrS"
                     /locus_tag="VGR22_00310"
     CDS             complement(628..2427)
                     /gene="thrS"
                     /locus_tag="VGR22_00310"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEV2127043.1"
                     /translation="MASSSAAVENMDIAVDPENNREYELARMRHSTAHLMAEAVQEIF
                     PDAKLGIGPAIADGFYYDFDLPRPLRPEDLEEIEKRMRKNQKQNEPYVRREISREEAL
                     EIFSGNPYKVEIINELPADEVISTYQQGHFLDLCRGPHVESTGKIGPFKLQSVAGAYW
                     RGDERRPMLQRIYGTAWPTQEELDEYLEKLEEAQRRDHRRLGKELDLFSVSEEIGPGL
                     ILWHPNGAMVRYLVEQFEQQEQLDRGYDLVYTPHIGSEKIYQKSGHLQTYIENMYSPM
                     SIEDVDYYLKPMNCPGHIMIYKSQVHSYRELPMRFAELGTVYRYERSGTLHGMLRVRG
                     FTQDDAHIFCTHEQVVDEVGGVIDLAIHMADIFGYGFEVYLGTRPEKAIGDEQVWDEA
                     TEALKRALSQRNLDYVVDEGEGAFYGPKIDIKWLDALGRAWTGPTIQVDFNLPERFDV
                     NFVGEDGERHRVAMIHRTLLGSMERFVGGLVEHYAGAFPSWLAPVQARVIPITDKQSE
                     YAEQVEQRLKDAGLRVEVDNSRDRMQAKIRNAQLQKVPYMLVVGGREADANAVAVRLR
                     SGEDLGAIPIDQFVDLVSPVIDTKSLDLQEPRR"
     misc_binding    complement(2485..2727)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2879..3322
                     /locus_tag="VGR22_00315"
     CDS             2879..3322
                     /locus_tag="VGR22_00315"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HEV2127044.1"
                     /translation="MGTRWFALVVGVVYLVIGIAGFIPALGVDRHTPDLAIESHYRDL
                     FGLFPINILHNVVHLSVGVLGIVAYRSFASARLYCKGLAIFYGVLAIMGMIPVLNTTF
                     DLIPIFSHDIWLHALTALAAAYSGWAVSEDDTGEPLRSDPPGTVR"
     gene            complement(3332..4459)
                     /locus_tag="VGR22_00320"
     CDS             complement(3332..4459)
                     /locus_tag="VGR22_00320"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEV2127045.1"
                     /translation="MRFFITVAILLLSIVLPTLSAASQEVPELQINSERYIVIDAKTG
                     QVYAQKGARDEVAIASLTKIFTAVQALEMAPPDTPITTTADDLFGSAHTLMGFGPNET
                     YTLEDMIYGMLLPSGNDAAHAIARSLGGQPGDTAEEAVQRFMDLVNQRVLDMGLTDTH
                     LITPSGWGVPGHHSSAWDVAAMMQHAMQYPLIEQAINTRSYTTSNGLLTVRNTNKLLN
                     SYSALLGGKTGYDLDAGWCLVNVAEIGDRRMIAVTLDGIAPDDWYDDNRVLLDYGFDQ
                     TAEVANWSGDVVVWQNPSIAQIERSASMDAALAGRSMAESNAVADVVDSPRVLAAERE
                     TASRLAAGSTAAVMVAIVALLGMRGLLAFRRWPLGRARPVS"
     gene            complement(4541..4614)
                     /locus_tag="VGR22_00325"
     tRNA            complement(4541..4614)
                     /locus_tag="VGR22_00325"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4578..4580),aa:Val,seq:gac)
     gene            complement(5028..5450)
                     /locus_tag="VGR22_00330"
     CDS             complement(5028..5450)
                     /locus_tag="VGR22_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127046.1"
                     /translation="MSDERRFEQQSQESEGRPAAEWITLGISIAFVAAMVALVTWFYF
                     RGAEEVPAIVAEAQLAEVSERNGSWYLPIEILNDGDATVADAIIRGELVTGSGEPQTA
                     EVTIDFLAGGEREQATLVFDSDPREGDLTVAPVSYENP"
     gene            complement(5447..6283)
                     /locus_tag="VGR22_00335"
     CDS             complement(5447..6283)
                     /locus_tag="VGR22_00335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02587 family membrane protein"
                     /protein_id="HEV2127047.1"
                     /translation="MSSDGRAAWSREINAYTRAISGAYIFGVPLLFTMEMWWIGEYID
                     RSKLLMAVGLAFAANFGLSWVSGFRRDSSLFASVEEAIEAVAVGTITASAMLLVLNRI
                     ALGDPIESVAGMILVQAVPLSIGASVANEVFGRPGNRRGKSGHQHLSAGKELLSDVGA
                     TMIGGVFIGFSIAPTEEVPMLAAALDYPHLLALVAFSLGVSYIIVFASGFDRPHPEGV
                     FQQPITETALAYVVSVVVALVVLYLFDRITFGQPLQSIAQQALVLAVPTTVGGAAGRL
                     VV"
     gene            complement(6406..6801)
                     /locus_tag="VGR22_00340"
     CDS             complement(6406..6801)
                     /locus_tag="VGR22_00340"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEV2127048.1"
                     /translation="MTATYDVIFDGGAKGNPGLGYGSYQITRDGAVIAHTQLNFGANV
                     TNNQAEYMTLLNALQWLADGLGNSASSASVTVHGDSQLVIKQLSGEWKIKDARLRRIA
                     METRAQMDRFHDVSLTWHRRDVSVQRLGH"
     gene            complement(6876..>8472)
                     /locus_tag="VGR22_00345"
     CDS             complement(6876..>8472)
                     /locus_tag="VGR22_00345"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEV2127049.1"
                     /translation="PIQFPIGAAENFRGIVDLLTGEALIMHENGDGGYDRAPIPEELA
                     DDVDTYRRALIESIAEHNEELLMRYLDEEPIDDEEIIRELHSCVEDGTVVPLLCGSAD
                     GNHGVSPLIEAMVRHLPSADERVEIAMKGDEEVQLKASPDESTVGLVFKSISDPHVGR
                     VSLVRVFAGAINAHDTLVNSRTGSKERIGKPFTLCGKTQVEMESIPAGDIGALTKLGD
                     VQTGDTLVANDHGPMLRPMESMEPTMALAVNPESKADVDKLAVALERMVAEDAGIRVR
                     RDEATGETLILGQGEQHINLAAERLMKRSGITISLTTPRVAYRETITKPTRAEYKHKK
                     QTGGAGQYGHVVLEVEPSENEFAFDEQVVGGAVPKGFFPAVEKGVRDGMESGSLAGFP
                     VVNVHATLVDGSYHSVDSNEMAFRIAAKECIRRALDQGSPVLLEPIHRLEVTVPDELM
                     GDVLGHLNSKRGQVLGVDAVRHGWSQITAEVPLAEVQRYSTELRSMSQGRATFTMRFA
                     RYQQVPETIAETVRKEAEEAKAA"
BASE COUNT         1501 a         2664 c         2655 g         1652 t
ORIGIN      
        1 gcgccgccgg gatcgccgca agctagaaca ctaggacgtt ggtggcttcg gcgcaacatt
       61 gtgcgaactc ggttggcgtc gagaaggagg cgttcttgcc ttcgagatcg gcgtcgctga
      121 ccccacgggc atgcgcgcaa ccacctcata atgaaaacgt ggacgtcctt ctccctcgct
      181 ttcgccagca gatccttcag cggcgggaag ccgatcggga gcactgcatc gatgatcgca
      241 tcgtgaatca ggaccactgc gtcacctgcc aggttgactt ccgcctcgat tcccgcttca
      301 gccgcatcgt tcaccgccaa gtggataagc atgcacgcct tggtcggatc atcaggacca
      361 tgcgtggcca gatagacgag ctccgccatt gccgcctccc tgcatgatgg tctcccgcaa
      421 agcggaaacc ttgtcacgat cgtcccgtcg agcaaggctc accgtgggtg atgttgatag
      481 gttatcggaa acgattatag caccgagttg gagtgacatc tggcctgacg gtggggtgga
      541 gagggaaaga cccgctctcc ccattggaag agcgggtccc tgccgagttg actccgagcg
      601 cgtgaatgag attgcgaatc tgcggcccta tcgacgcggc tcctggaggt cgagtgattt
      661 cgtgtcaatg accgggctga cgagatcgac gaactgatcg atggggatcg cgcccaggtc
      721 ctcgcccgag cgaagccgaa cagcgacggc attcgcatct gcctcgcgcc caccaactac
      781 aagcatgtac ggcaccttct ggagctgtgc gttccggatc tttgcctgca tacggtcgcg
      841 cgagttgtcg acctcgacgc ggagcccggc atccttgagg cgctgctcga cctgctccgc
      901 gtactcggac tgcttgtccg tgatcgggat gacgcgcgcc tgtaccggcg caagccacga
      961 cgggaaggcg ccggcgtagt gctcgaccag accaccgacg aagcgctcca tggagccaag
     1021 cagcgtgcga tggatcatcg cgacgcgatg ccgttcgcca tcctcgccca cgaagttaac
     1081 gtcgaagcgc tccgggaggt tgaagtcgac ctgaatcgtc gggcccgtcc acgctctgcc
     1141 cagtgcatcc aaccacttga tatcgatctt gggaccgtag aaggcgccct cgccctcatc
     1201 gacgacgtag tcgagattcc gctggctgag ggcacgcttg agcgcctccg tcgcttcatc
     1261 ccagacctgc tcatcgccga tcgccttctc tggccgcgtt ccaaggtaaa cctcaaagcc
     1321 gtagccgaag atgtcggcca tgtggatggc gaggtcgatt acgccgccga cctcgtcgac
     1381 cacctgctca tgcgtgcaga aaatgtgcgc gtcgtcctgg gtgaagccgc ggacgcgcaa
     1441 catgccatgc agcgtaccgg atcgctcgta gcggtagacc gttccaagct ccgcgaagcg
     1501 catcggaagc tcccggtagg aatgaacctg gctcttgtag atcatgatgt ggccgggaca
     1561 gttcatcggc ttgagatagt aatcgacatc ctcgatgctc attggcgagt acatgttctc
     1621 gatatatgtc tgcaagtgcc cggatttctg gtagatcttt tccgatccga tgtgcggcgt
     1681 gtagacgaga tcgtagccgc gatcgagctg ctcctgctgc tcgaactgct caaccagata
     1741 gcgtaccatc gcgccgttcg ggtgccagag gatcaggccc ggtccgatct cttcggacac
     1801 cgagaagagg tcgagctcct tgccgaggcg ccggtggtcg cgccgctgcg cctcctccag
     1861 cttctccagg tactcgtcga gctcctcctg cgttggccaa gccgttccgt agatgcgctg
     1921 caacatcggc cgacgctcgt cgccacgcca gtaggcgcca gcgacactct ggagcttgaa
     1981 tggcccgatc ttgcctgtcg actcgacgtg cggtccgcgg cagaggtcaa ggaagtgccc
     2041 ttgctgatac gtcgaaatca cttcatccgc gggcaactcg ttgatgatct cgaccttgta
     2101 cgggttgccg ctgaagatct cgagcgcctc ctcgcgcgag atctccctgc gaacgtacgg
     2161 ctcgttctgc ttctggttct tccgcatacg cttctcgatc tcctcgaggt cttccggtct
     2221 gagtgggcga gggagatcga aatcgtagta gaagccgtcg gcgatggcgg gaccgatacc
     2281 caacttggcg tctgggaaga tttcctggac ggcttcggcc atgaggtgcg ccgtcgagtg
     2341 gcgcatgcgc gcaagctcgt actcccggtt gttctcgggg tcgacggcga tgtccatgtt
     2401 ctcgacagct gcgctgctgc tggccatgtt gctgctcctg taatgtgtct gatgatcatg
     2461 agacgcaaga aacccggcgc cccgcaaagg gacgccgggt tggcgtggtt ccaccctcat
     2521 tccgacccgc cgacgcgaca tcgcgtcaag ggggtcgctc atggtgcccg gtaacggggg
     2581 cgcccggagc gcgctactgc cgcctgcggc aggttcacgg ctcggctcgt ggagggtttt
     2641 ccgcctgtac tctgccagtc actcgcaccg accgtgactt ctctgaggcg aactggcagg
     2701 cgtactcgtt ccagtcatcg ccgttgacgg ttactctcgc agtgtagtag cgggcccggt
     2761 acacgtcaac aggcgttccc tgcgagtgct acagccggcc ggcaccgcgc actggcactg
     2821 cgtttgcggt tttctgtccg ataccgatgg atggaacgac gcgcagagga gcattggcat
     2881 ggggacacgg tggttcgcgc ttgttgttgg tgtggtgtat ctggtcatcg gaattgccgg
     2941 gttcattccg gcactcgggg ttgaccggca cacgccggac ttggccatcg aatcgcacta
     3001 ccgcgatctt ttcggcctgt tcccgatcaa tatcctgcac aacgtggtgc atctgtccgt
     3061 cggcgtgctg ggcattgttg cgtaccggag cttcgcctcg gcgcgcctgt actgcaaggg
     3121 tctggcgatc ttctacggcg tgctggcgat tatgggaatg atcccagtgc tcaacaccac
     3181 gttcgacctg atcccgatct tcagccacga catctggctg cacgcgctga ccgcgctggc
     3241 cgccgcgtac tctggctggg cagtcagcga ggacgacaca ggtgagccct tgcgttccga
     3301 cccgccaggc acggttcgat agcgatggcg ctcaggacac tggtcgtgcc ctgccgagcg
     3361 gccagcgcct gaatgcgagc aggccgcgca tgccgaggag agcgacgatc gcgaccatca
     3421 ctgcggcagt actgccggct gccagacggc tagctgtttc gcgctccgcc gccaggactc
     3481 ttggagaatc gaccacgtcg gcgacagcgt tcgattccgc catcgagcga ccggcaagag
     3541 cggcgtccat cgacgccgag cgctcgatct gggcgataga cggattctgc caaaccacga
     3601 cgtcaccgct ccagttggcg acttcggccg tctgatcgaa gccgtagtcg agaagcacac
     3661 ggttgtcatc gtaccagtcg tccggagcga tgccgtcaag tgtgacggcg atcattcgcc
     3721 ggtccccgat ttccgcgaca ttgaccaggc accagccggc atcgagatcg tatccggtct
     3781 tgccgccgag gagtgcgctg taggagttga gcagcttgtt ggtgttcctg accgtgagca
     3841 ggccattgga cgttgtgtag gagcgtgtgt tgatggcctg ttcgataagc ggatactgca
     3901 tcgcatgctg catcatcgct gccacgtccc aagccgacga gtggtgccca gggacgcccc
     3961 atcccgatgg ggtgatgagg tgcgtgtcag tgaggcccat gtcgagcaca cgctggttga
     4021 ctaggtccat gaatcgctgc accgcctcct cggcagtgtc gcccggctgc ccaccgagcg
     4081 accgggcaat cgcgtgtgcg gcgtcattgc cggatggcag cagcatgccg tagatcatgt
     4141 cctcgagcgt gtacgtctcg ttgggcccga agcccatcag cgtgtgggcc gagccgaaga
     4201 ggtcgtcggc cgttgtcgtg atcggtgtgt cgggcggggc catctcgaga gcctgaacag
     4261 ccgtgaagat cttggtgagg ctggcgatcg ccacctcatc gcgcgcgcct ttttgggcgt
     4321 agacctgccc tgtcttggcg tcgatgacaa tataccgctc ggagttgatc tgcagctccg
     4381 gtacctcctg gctggcagcc gaaagtgttg ggagcacaat cgagagcagc aaaatggcga
     4441 ctgtgatgaa gaagcgcatg gtataggcgg gtcctcaccg gttcggcgag tggaatcaag
     4501 tagagccggc acctcacggg gaggcccggc tcctgggcgg tgggcgatag tggactcgaa
     4561 ccactgacct ccacgatgtc aacgtggcgc tctaaccagc tgagctaatc gcccgacagc
     4621 gcacagcgtg agaagtatag tggcgtctca accgccggtc aagcgcaatg tgtagggatg
     4681 cctcgcgggg agcaccggca tccctggccc gcaccggtgg ctggtgaatt gctgagcgag
     4741 cggttgacga tggcagcgag aagccgcgat cgaggcaagc gctcagaggc acggggagag
     4801 gatagcgcag gtgatgatcg agttgtcgtt tctcaccgcc atggggcaag gatgccgccg
     4861 atgatcgctc tacccatggg aaacagggcg aagatcagaa ctttctggag cagatcaggc
     4921 tcaacacgtc tcctggtgcg cgctgggtcg gctgtgcttc gcggccggta cccccgtctg
     4981 ctccgaacgg tcgcaagact ccgtccagac gacccgcccc cgcttgttca cgggttctca
     5041 tagctgacag gggcgacggt gagatcgccc tcacgtggat cgctgtcgaa gacgagtgtg
     5101 gcctgctcac gctcgccgcc tgccaggaag tctatggtca cctcggccgt ttgcggctct
     5161 ccgctaccag tgacgagctc gcccctgatg atggcgtccg ccacggtggc gtcaccgtcg
     5221 ttcagaattt cgatcggcag gtaccagctg ccgttccgct cgctgacttc ggcgagctgc
     5281 gcctcggcca cgatggccgg cacctcttcg gcgccgcgga agtagaacca tgtgaccagc
     5341 gccaccatag cggcaacgaa agcgatactg ataccgaggg tgatccactc ggcggccggc
     5401 cttccttcgc tctcctggct ctgctgctcg aagcgcctct cgtcactcat accaccagtc
     5461 tcccggccgc tccgccgacc gttgtcggta cggcgagcac cagcgcctgc tgcgcgatcg
     5521 actgcagcgg ctgcccgaag gtgatgcgat cgaacagata cagcaccacc agcgcgacga
     5581 ccaccgacac gacgtacgcg agcgccgttt ccgtaatcgg ttgctggaaa actccctcgg
     5641 ggtgcggccg gtcgaagccg ctggcaaaga cgatgatgta gctgactccg agactgaacg
     5701 cgacgagggc aagcaggtgc gggtaatcga gcgccgcggc cagcatgggc acctcctcgg
     5761 tcggagcgat cgagaagcca atgaagacgc caccgatcat ggtggctccc acatcggaga
     5821 ggagttcctt ccctgccgac agatgctggt ggccgctctt gcctcgccgg ttgccgggtc
     5881 taccgaagac ctcgtttgcg acggaggccc cgatgctgag cggtaccgcc tggacgagaa
     5941 tcatgccggc cacgctttct attgggtcgc ccagtgcgat gcgattgagc acgagcaaca
     6001 tggcggaggc ggtgatggtg ccgacggcga ctgcttcgat cgcttcctca acacttgcga
     6061 atagcgaact gtcacggcgg aaaccggaca cccacgagag gccgaagttt gccgcgaagg
     6121 ccaggccaac ggccatcagg agtttcgagc ggtcgatata ctcgccgatc caccacatct
     6181 ccatggtgaa cagcagcgga accccgaaga tgtaggcgcc ggagattgcc cgggtgtagg
     6241 cattgatctc ccgcgaccag gccgctcttc catcactcga cacgcgttgt tctccagaca
     6301 ttcaaaccgc tgaggctggc ggcaggggct gtatcgcacg gtgggagggg cacgaaaggg
     6361 acggcaatca tgtcaacggc cgtgcttcgc aatggtgacg aggtgtcagt ggccgaggcg
     6421 ctgcaccgac acatcgcgcc ggtgccaagt cagtgagaca tcgtggaagc ggtccatctg
     6481 ggcacgggtc tccatggcga tacgtcggag acgggcatcc ttgatcttcc actcgcccga
     6541 gagctgcttg atgaccagct ggctgtcgcc atgcacggtg acactggcac tgctggccga
     6601 gttgccgagc ccgtcagcga gccactgcag cgcgttgagc agcgtcatgt actcggcctg
     6661 gttgttggtg acattggcac cgaagttcag ctgagtgtgg gcgatgacgg cgccgtcacg
     6721 ggtgatctgg tagcttccgt agccaagccc gggattgccc tttgcgccgc catcaaagat
     6781 tacgtcgtag gtcgccgtca cgtgtccatc cttgcgaaac cagcgtgtcg ttcatttcgc
     6841 aaggatggta cacggtcagc gcggcaccga gcacgtcagg cggccttggc ctcctccgcc
     6901 tctttccgga cggtctcggc gatagtctcc ggcacctgct ggtagcgcgc gaagcgcatg
     6961 gtgaacgtgg ctcgcccctg cgacatcgat cgaagctcgg tcgagtagcg ctggacctcg
     7021 gccaatggca cctcggcggt gatctggctc catccatggc gaacggcatc cacgccgagc
     7081 acctgcccgc gcttgctatt cagatgtcca agcacgtcgc ccatcagctc atccggcacc
     7141 gtcacctcga gccggtggat cggttcaagc agtaccgggc tgccctggtc aagagcacgg
     7201 cggatgcatt ccttcgccgc aatccggaac gccatctcgt tcgagtccac cgagtggtaa
     7261 ctgccgtcga cgagggtcgc gtggacgttg acgacgggaa agccggccag cgatcccgat
     7321 tccatcccgt cgcgcacgcc cttctcgacc gccgggaaga agcccttcgg cacagcgccg
     7381 ccgacgacct gctcatcgaa ggcgaactcg ttctcgctgg gctcgacctc cagcacgacg
     7441 tggccgtact ggccagcacc gccggtctgc ttcttgtgct tgtactcggc acgggtcggc
     7501 ttggtgatcg tctcacgata ggcgacgcgg ggcgtggtga gcgagatcgt gatgccggac
     7561 cgcttcatga gccgctcagc cgcaaggttg atgtgctgct cgccctgccc gagaattagc
     7621 gtctcgccgg ttgcctcgtc gcgtcgaacc ctgatgccgg catcctcggc caccatccgc
     7681 tcgagcgcga cggccagctt gtcgacgtcc gccttggact ccgggttgac ggccagcgcc
     7741 atcgtcggct ccattgattc catcggtcgg agcatgggac cgtggtcatt ggcgacgagc
     7801 gtgtcgccgg tctggacatc tccgagcttg gtaagtgcgc cgatatcccc tgcgggaatc
     7861 gactccatct cgacctgcgt ctttccgcac aaggtgaacg gcttgccaat gcgttccttg
     7921 cttcccgtcc tagaattgac caacgtatcg tgcgcgttga tcgcaccggc gaagacgcga
     7981 accagtgaaa cgcgcccaac gtgcgggtcg ctgatcgact tgaaaacgag gccgacggtc
     8041 gactcgtctg gcgatgcctt gagctgtacc tcctcatcgc ccttcatcgc gatctcgacc
     8101 cgctcgtcgg cgctcgggag gtgccgcacc atcgcctcga tcagcggcga gacgccgtgg
     8161 ttgccgtcgg cggagccaca gagcaggggc accaccgtgc cgtcctcgac acaggaatgc
     8221 aactcgcgga tgatctcctc gtcgtcgatc ggctcctcgt cgaggtagcg catcagcagc
     8281 tcctcgttgt gctctgcgat cgactcgatg agtgcgcggc ggtaggtgtc cacatcatcg
     8341 gcgagttctt ccgggatcgg cgcgcggtca tagccgccgt ccccgttctc gtgcatgatc
     8401 aacgcctcgc cggtgagcag gtcaacgata ccgcggaagt tctccgccgc accgatcgga
     8461 aactggatcg gc
//
LOCUS       DASYWL010000011         7475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_281821, whole genome shotgun sequence.
ACCESSION   DASYWL010000011 DASYWL010000000
VERSION     DASYWL010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7475
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281821"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(288..1832)
                     /locus_tag="VGR22_00350"
     CDS             complement(288..1832)
                     /locus_tag="VGR22_00350"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2127050.1"
                     /translation="MSPGYRWAATRTKIGAIIMDIAGIHPHQNMRSGAPSPARHAHSV
                     QFYDDGTALVSRVSDFTSEALHSGGTAVVIATPAHRDAIAARLVAAGHDLARARQTGR
                     YTSLDAARTLANTMVNGQVDELRFDAVIGGPVRQATALAARTGGITAVFGELVALLAL
                     EGDHEAAITMEGWWNTLSRRHGFALHCTYPMHAFSQTDDGDVLGRICDAHTDVVPAES
                     YTTLHTPDEQSRQIVLLQQKASALEQEVIARARAEEALQAQYRELQAALIAREEFLAV
                     AAHELRTPMTGLRGNAQMLRRSMQQGREVSRERMKRSLDTIENLSLRLNHLITRLLDA
                     SQIEAGMLRLAPARTDLVALVRSAMDHQPAHDGHMVEFDGPDRLDLVVDAVRIEQVIA
                     NLIDNSIRYSPDGGTVSVKVEDYGDEGIELSVTDTGPGIPPDQREAVFTSYRQGGSAE
                     RFAGIGLGLFVSRQIVELHGGAMHIEDPADHPGTRIVITLPSAASSTRTGESLAESLP
                     GSYSAD"
     gene            complement(1932..3026)
                     /locus_tag="VGR22_00355"
     CDS             complement(1932..3026)
                     /locus_tag="VGR22_00355"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEV2127051.1"
                     /translation="MSCFRWQAVCSSDIFPSPRNRKGTPLNLSLAHTGRRLLGRYGAW
                     IWLALVLGATVWFGWRQQVEIRNIWHSLRTADPTWIAALVGIEIVILALITITYRSLL
                     LRLGHEVRFSSLINVHLERIVVGTVTPVGGPSSMAIFVHRLRQRGVKPADALLTVSIK
                     SVIGNFAFLLLLVPVLFVQKPSTLLLLSTGALILLVISMAWLLHTALSRKKPPRWIIS
                     RLPRKALRFLAEVRSHQISLGSLIGPFFYMLATKLGGVAMLFIALKAVGHAPNIQVPL
                     MAYVVGMVFLLVAPVFQGIGVVEVSMAVALQQMGVPTAAAVSAVLLTRVGELWLPLLT
                     GVLVQLGEILAAHRHPRPEPQPVPMKVRAR"
     gene            complement(3156..3578)
                     /locus_tag="VGR22_00360"
     CDS             complement(3156..3578)
                     /locus_tag="VGR22_00360"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEV2127052.1"
                     /translation="MFGTLHSDEIEDILFTYHTGHLACIAAGAPYIVPVTYAYDGGAL
                     YGAITPGCKITALRDNSAVAFGIADLSEPHRWRSVIIEGTWSEVTDPHEREQTIALLA
                     NVDQPIELDESSILFRVSPQQRSGRWLETPHRHTAMRH"
     gene            complement(3910..4227)
                     /locus_tag="VGR22_00365"
     CDS             complement(3910..4227)
                     /locus_tag="VGR22_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127053.1"
                     /translation="MEHPERDRLPERHRLRAPRHAHAPVLLLRQPPPLAAKRIGQAIE
                     QRPHLLSGDRQHKPRETERRGRLRQLGRVRRRNLRGAARRREDGEADDEQDHTTSHHR
                     LLS"
     gene            4598..5554
                     /gene="thyX"
                     /locus_tag="VGR22_00370"
     CDS             4598..5554
                     /gene="thyX"
                     /locus_tag="VGR22_00370"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEV2127054.1"
                     /translation="MTDTQPEATVEPRQTAEALQRVSIPELDAVLGKPFPVLDQGFVR
                     VIDYMGTDSSIIQAARVSYGAGTKKVREDRGLIRYLMRHDHTTPFEMCDIKLHVKVPM
                     DIWRQWIRHRTASVNEYSTRYSIAIDEAHRVQDGEWRGQSASNRQGSEGVVDSSLIGR
                     LSEREAELQELARQVYRERLELGVAREQARKDLPLSTYTEAYWKTNLHNLLHFVHLRM
                     DEHAQQEIRDYARTIGEEIIRRWCPVAWEAFLDYRVNSMGFSAPELACIGPIVAGDRE
                     AAIGAALEQGLLRDRKGERAATREGQEFAVKLARFGLQAPWN"
     gene            complement(6124..6984)
                     /locus_tag="VGR22_00375"
     CDS             complement(6124..6984)
                     /locus_tag="VGR22_00375"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HEV2127055.1"
                     /translation="MSTTTNGEPTRETVREHYAAAAMEVSTTSGGCCLPDRTDPITSN
                     LYTADELASLPQEASLASLGCGNPTALTILEPGETVLDLGSGGGIDVLLSARRVGPDG
                     FAYGLDMTDEMLALAERNKARSELTNVEFLKGHIEAIPLAECTVEVVISNCVINLSTD
                     KGQVLREAFRVLRPGGRFAVSDVVTRGELPADLRVDMEAWIGCVAGALDEEEYRQLLA
                     DAGFENVEVQVTRVYDPRELIASLGSGSGCCGGDERMPVWSEADLERLAASGGQVMSA
                     FVRAHKPSAA"
     gene            complement(7052..7306)
                     /locus_tag="VGR22_00380"
     CDS             complement(7052..7306)
                     /locus_tag="VGR22_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127056.1"
                     /translation="MFDINDALVHQIIRSAHEEKPASRGAFQMASHPLNTRSLPTITI
                     PSPVAVLTTITAGFRADVARLTALAAPQRVAEEACCPAAA"
BASE COUNT         1234 a         2340 c         2621 g         1280 t
ORIGIN      
        1 ttggtgggat ctacgcactg ctgatgacgg tcgccgttgc ctacctcggc gagcacttca
       61 tcctcgacgc ggcggtgggc tggctgatcg cccttccggg ttggtgggtg acccgaggtg
      121 tggttgtcga ggcgtactct gtttcccatg caggacactg aggcccgggg gctgcccgtt
      181 gatgtcgcga cggtcccggt tttagcccac ggtctgttcg ggactctcat catcctgcca
      241 ctggtcggga cccggcgttc cctgggtggt cgtcgaggtc tggaacgtca gtccgcgctg
      301 taggagccgg ggagcgactc ggccagcgat tccccggtgc gggtggagga ggcagcggac
      361 gggagggtga tgacgatccg ggtaccgggg tggtccgccg ggtcctcgat gtgcatcgcc
      421 ccgccgtgca gctcgacgat ctggcggctg acgaacagcc cgagaccaat tccggcgaat
      481 cgctctgccg agccgccctg acggtagctg gtgaagaccg cctcgcgctg gtcgggcggg
      541 atgcccgggc cggtgtcggt caccgaaagc tcgatgccct cgtcgccgta gtcctcgacc
      601 ttcacggaca cggtaccgcc gtccggactg tagcggatgc tgttgtcgat caggttggcg
      661 atgacctgct cgatccggac agcatcgacg accaagtcga gtcggtccgg gccgtcgaac
      721 tccaccatgt gaccgtcgtg tgcgggctgg tggtccatcg ccgaccgcac cagcgccacc
      781 agatcggtcc gcgccggggc cagccgcagc atgccggcct cgatctggct ggcgtcgagc
      841 agccgcgtga tcaggtggtt gaggcgcaag ctcaggttct cgattgtgtc gagcgagcgc
      901 ttcatccgct cgcgactgac ctcccggccc tgctgcatgc ttcgacgcag catctgcgcg
      961 ttgccacgca ggccagtcat cggggtgcgc agctcatgcg ctgccaccgc gaggaattct
     1021 tcgcgggcga tcagcgccgc ctgcagctcc cggtactgcg cctggagcgc ctcctcggcg
     1081 cgagcccgcg cgatcacctc ctgttccaaa gcgctggcct tctgctgcag gaggacgatc
     1141 tggcgcgact gctcgtcggg ggtgtgcagg gtggtgtagc tctcggccgg aacgacgtcg
     1201 gtgtgggcgt cgcaaatgcg gcccagcaca tcgccgtcat ccgtctgact gaacgcgtgc
     1261 atggggtagg tgcagtgcaa tgcgaagcca tgcctgcggg agagggtgtt ccaccagccc
     1321 tccatggtga tcgctgcctc gtggtcgccc tccagtgcga gcagggcgac cagctcgccg
     1381 aagactgcag tgatcccgcc agtgcgggca gcgagtgcag tcgcctgacg gaccgggccg
     1441 ccgatgaccg catcgaagcg gagctcgtcg acctgaccgt tcaccatcgt gttcgcaagg
     1501 gtccgggctg cgtcgaggga ggtataccgg cccgtctggc gggctcgggc gagatcatga
     1561 ccggcggcga ccagccgggc ggcgatagca tcgcggtggg cgggcgtcgc gatcacgacc
     1621 gcagtgccgc cggagtgcaa ggcctcgctg gtgaaatcac tcacccggct gaccagggca
     1681 gtgccatcgt catagaactg caccgagtgc gcgtgtcgcg ctggactcgg tgcaccggat
     1741 ctcatgttct ggtgcgggtg gatgccagcg atgtccatga tgatcgcacc gatcttggta
     1801 cgcgttgctg cccagcggta tccgggcgac acaatgctat cgtgacaggt cacacagtga
     1861 cagagacgat aatcccttcg atagtcgtgt acgttctgtg ccgctggtat tctgcattgc
     1921 catcgaacgg gctagcgtgc ccgaaccttc atgggtaccg gttggggctc gggtcgtgga
     1981 tgacgatgag ccgcgagtat ctcgccgagc tgaaccagca ccccggtcag cagcggcagc
     2041 cacaactcgc cgacacgcgt cagcagcacc gcactgacgg ctgcggcggt gggaacgccc
     2101 atctgctgca gggcgacggc catgctgacc tcgaccacgc cgatgccctg gaagactggc
     2161 gcgacgagca ggaagaccat gccgaccacg tacgccatca gcggcacctg gatgttcggt
     2221 gcgtgcccga ccgccttcag cgcgatgaag agcatcgcca cgccaccgag cttggtcgcc
     2281 agcatgtaga agaaggggcc gatcagcgag cccagactga tctggtggct gcgcacctcg
     2341 gcgaggaaac gaagcgcctt gcgcggcagc cgggagatga tccaccgggg tggcttcttg
     2401 cggctcaacg cggtgtgcag cagccacgcc atcgagatca ccagcaggat cagggcgccg
     2461 gtgctcaaca ggagcagggt ggagggcttc tgcacgaaga ggaccggcac cagcagcaac
     2521 aggaacgcga aattgccgat cacgctcttg atggagacgg tcagcagcgc gtccgcaggc
     2581 ttcaccccgc gctggcggag gcgatggacg aagatcgcca tggacgacgg accgccgacc
     2641 ggcgtcacag tgccgaccac gatccgctcc agatgcacgt tgatgaggga ggagaagcga
     2701 acctcgtggc ccagccgcag cagtagtgat cggtaggtga tggtgatgag cgcgaggatg
     2761 acgatctcga tgccgaccag cgcggcgatc caggtcgggt ctgcggtgcg gagcgagtgc
     2821 cagatgttcc ggatttccac ctgctgccgc cagccgaacc agacggttgc cccgagcacg
     2881 agcgcgagcc agatccaggc gccgtagcgc ccgaggagtc gcctgccggt atgtgcgagt
     2941 gagaggttca gtggagtccc cttcctgttg cgcggtgacg ggaaaatatc gctgctgcac
     3001 accgcctgcc atctgaagca cgacataagg ctggactcgc cttgtgccag tcccagatgc
     3061 aaactgcatg ccaccggaca ggatcatacc cgatggggga tggcatggct ccatcgccct
     3121 gtgcagcctt gctggctgtg gatgctggct cttcgtcagt gcctcattgc cgtgtgacga
     3181 tgtggtgtct ctagccaccg cccgcttcgc tgctggggtg agactcggaa caggatactg
     3241 ctctcgtcca gctcgatggg ctggtcgaca ttcgccagca acgcgatggt ctgctcgcgc
     3301 tcgtgcgggt cggtgacctc cgaccaagta ccctcgataa tcacactgcg ccagcgatgt
     3361 ggttcggaga gatcggcgat cccgaaggcg actgccgagt tgtctcgcag cgccgtgatt
     3421 ttgcacccgg gcgtgatcgc accgtagagg gcgccgccat cgtaggcgta ggtgaccgga
     3481 acgatgtagg gagcgccggc ggcgatgcag gcgaggtggc cggtgtggta agtgaagagg
     3541 atgtcctcaa tctcgtccga gtgcagggtg ccgaacatgc cattactcct tccggtggcg
     3601 tgcaatcgtg gcggccttcg tcagccaggg gcttggggcc atcatgcctc gacgactgtg
     3661 tcgaatgcat tacatcgaga gcgacggccc ttgcagtttc gtgtcagttc ggttgccgct
     3721 ccctgctcgc ggacagggcg gcgtcgagga cagacagccg ggagaaggcc atggcgtcgt
     3781 cctcgctgct caagaaacga acgggtcaga ctgtgagcca gcgtcggttg tggtcgcggg
     3841 tatgcgtgtg acaggagtgt ggaggcagat ctatcgcgcg atccggtgga catggccatc
     3901 acgagccact cacgaaagga gccggtgatg cgacgtcgta tggtcctgct cgtcatcagc
     3961 ctcgccatcc tctcggcgcc gggcagcgcc gcgcaggttg cgacgcctga cccggccgag
     4021 ctgccggagg cgaccgcgcc gttcggtctc tcggggcttg tgctgcctgt caccggagag
     4081 gaggtgcggg cgctgctcga tcgcctgccc gatacgcttg gcggcgagcg gcgggggctg
     4141 gcgcaggagg aggacgggcg cgtgcgcgtg gcgtggggcg cggagtcgat gccgttcggg
     4201 cagccgatca cgctcggggt gctccacttt ccgacgagcg atttctttcc cgccgatttc
     4261 accgccggga actacgttgc ggtgacgatg ctgaccgacg acgcgcaggt ggccgccggc
     4321 ggccgggacg gcgaccttgt gtgggtgtgg gcggagatct cggtcggggc cgttggcccc
     4381 agcgacgcga ctccggcggc gcgtggctct ttaccgctgc cgccgatacg ccgcagcggc
     4441 tcaagacact tgtggggcgt tcgtcgaggc gacggatggc gtggagggtg ccacaccagc
     4501 ggcgtgacca ccccggataa tctatggaag atcgggtgca tgtgtggtcc aatcgtgctg
     4561 acacgagccg tccgtacagc gcgaaggacc accagccatg accgacactc agcccgaagc
     4621 cacggtcgag ccccggcaga ccgccgaagc cctccagcgc gtctccatcc ccgagctcga
     4681 tgcggtgctc ggcaagccct tcccggtgct cgatcaggga ttcgtgcggg tgatcgacta
     4741 catggggact gactcctcga tcatccaggc ggcgcgcgtc tcctatgggg cgggcaccaa
     4801 gaaggtgcgg gaggaccgcg ggctgatccg ctacctcatg cgccacgacc acacgacacc
     4861 gttcgagatg tgcgacatca agctgcatgt caaggtgccg atggacatct ggcggcagtg
     4921 gatccggcac cgcacggcca gcgtaaacga gtactcgacg cgctactcga tcgcgatcga
     4981 cgaggcgcac cgggtccagg atggggagtg gcgcggacag tcggcctcca accggcaggg
     5041 gagcgagggg gtggtcgaca gcagcttgat cggccggctc tcagagcggg aggcggagct
     5101 gcaggagctc gcgcggcagg tctaccggga gcgactggag ctgggggtcg cccgggagca
     5161 ggcgcgcaag gacctgccgc tctcgaccta caccgaggcc tactggaaga ccaacctgca
     5221 caatttgctg cacttcgtgc acctgcgcat ggacgagcac gcccagcagg agatccggga
     5281 ctatgcccgt accatcggcg aggagatcat ccgccgctgg tgtccggtgg cgtgggaggc
     5341 cttccttgac taccgcgtca acagcatggg cttcagcgca ccggagctgg cctgcatcgg
     5401 cccgatcgtc gccggggacc gggaggcggc gatcggcgca gcgctcgagc aggggctgct
     5461 gcgggaccgc aagggggagc gggcggccac ccgcgagggc caggagttcg cggtcaagct
     5521 cgcgcgcttc gggctccaag cgccatggaa ttaggtgagc gcgtggggga tgcgcggaag
     5581 cggatagtcg gccggtgaag gctgccaacg gaaaaacgtg gcagtgtggc agccttggac
     5641 gatttgcctg taatgacggg gaaaatcgag tctgccactg ttcggcggat aggtggcagc
     5701 cctccataaa cacggggtgt cgttcgccct ctgtgtatgc cgtgcgtagc gtctctaccc
     5761 aatacgaacg catcgaatgg cgggacttgg cgcatccgtg acgcgccaac ccccacgtcg
     5821 tcaggccggg acgggccggt acgaggccga cgctacggtt ggagaggtgg ttgggcgtga
     5881 ccccgggcgg atatgcctct gggcggctgc gccgcgggat cagtccgcag ggatcggcct
     5941 tgtgccggac cgagcagggg cagaccccgt gtctacccct gcggatggcc gcgctcgccg
     6001 tctgttttcc cggatttcgc ctgagctggc acttgggcga cattggacgc gcgggacggt
     6061 gacgtgtgtg gggccggtag gaaacgcatc caagttgtcc gctacaggcg ccttgcagcc
     6121 cgttcaggcg gcgctcggct tgtgggcccg gacgaaggcg ctcatcacct ggccgccact
     6181 cgccgcgagc cgctcaagat cggcttcgct ccatacgggc atgcgctcgt cgccaccgca
     6241 gcagcctgac ccgctgccga ggctggcgat caactcccgt gggtcgtaga cgcgggtcac
     6301 ctgcacctcc acgttctcga acccagcgtc ggccagcagc tgccggtact cctcttcgtc
     6361 gagcgcgccg gcgacgcagc cgatccatgc ttccatgtcg acgcgcaggt cggcgggcag
     6421 ctcgccgcgg gtgacaacgt ccgagactgc gaagcggccg cctggacgca gcacacggaa
     6481 tgcctcgcgc agcacctgtc ccttgtcagt ggaaagattg atcacgcagt tggagatcac
     6541 gacctcgacc gtgcactccg ccagcgggat cgcttcgatg tggcctttca ggaactcgac
     6601 gttggtcagc tcggaccgcg ccttgttgcg ctcggcgagc gccagcatct cgtcggtcat
     6661 gtcgagcccg taggcgaagc cgtccggacc gacccgtcgc gccgagagca gcacgtcaat
     6721 gccgccgccg gaaccaaggt caagcaccgt ctcgccaggt tcaaggatgg tcagggcggt
     6781 tgggttgccg cagccgagtg acgccaggga ggcctcctgc ggcaggctgg cgagctcatc
     6841 ggccgtgtag aggttggagg tgatcgggtc ggtgcggtca ggcaagcagc agccaccgga
     6901 ggtggtgctg acctccattg cggcggcggc ataatgctcc cgcacggtct cgcgggtcgg
     6961 ttcgccattc gtggtcgtgc tcatcgttgt catctccctc gggcgagcgc acgcttcggt
     7021 cctggctcct gcggctcacc cggcgtgcgt gctacgccgc ggccgggcag caggcctctt
     7081 cggcaacccg ctgcggggcg gccagcgcgg tcagccgggc gacatcggcg cggaaacccg
     7141 ccgtgattgt ggtcagcacg gcgacaggtg acggtatggt gatcgtcggc agcgagcggg
     7201 tgttgagcgg gtgcgatgcc atctgaaagg cgccgcggct ggcgggcttc tcttcgtgag
     7261 cggagcggat gatctggtgg acgagcgcgt cgttgatatc gaacatcgtg gtaatctctc
     7321 tcgctatcac atcgacattc gtctatgtga gcggccaaag aaaacgcggg tcagcgaaga
     7381 gccgccgcgc gctcgggagc gatctgctcc cgcgagccct gaagaaacga gagcccgtcc
     7441 tcgttggccg cgtagtacgc ccaaattcct tggcg
//
LOCUS       DASYWL010000012         8244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_150484, whole genome shotgun sequence.
ACCESSION   DASYWL010000012 DASYWL010000000
VERSION     DASYWL010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8244
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150484"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VGR22_00385"
     CDS             complement(<1..524)
                     /locus_tag="VGR22_00385"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEV2127057.1"
                     /translation="MWLLTLILAILFGLGAFMAPVEAVSRVGVGQTSVSPLTVEGEPV
                     TALGVEPVSGEARYLGTGGATWTHDETGGWRRTGVAPGHHVMVVDSRDDLLVWAGTPV
                     DCYRGVEAPAPMVRSTDGGATWVEAGPAGAVPLASWMNTGIAVAHDCSGLLLSTDSGT
                     TWSVPEGFPLGSQVT"
     gene            complement(597..1673)
                     /gene="aroC"
                     /locus_tag="VGR22_00390"
     CDS             complement(597..1673)
                     /gene="aroC"
                     /locus_tag="VGR22_00390"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEV2127058.1"
                     /translation="MGSRFGTIFSLTTWGESHGPALGVVVEGCPAGIPLTVEDIQHEL
                     DRRRVGQSKVTTPRNEKDRAEILSGVFEGVTTGTPISIITYNADVDSSKYENLRNLFR
                     PGHGDYTYQMKYGHRDHRGGGRSSARETWGRVAAGAIARKILAHAGAHVFGFTREIGG
                     IEMETFDEAEIERNIVRCPDPVAAEKMIDAIMKVKEEKNSLGGVVEARAIGVPPGLGE
                     PTFDKLDALIGQAMLSIPATKGVEIGRGFEQARLTGRESNDPFGIRDGRVRTESNNAG
                     GTLAGISTGEEIVVRVAIKPTSSIEQDQSTVTTELQPTTVKVEGRHDPSVVPRAVPVV
                     EAMLANVLADLYLRNRASRIDWPD"
     gene            complement(1673..2593)
                     /locus_tag="VGR22_00395"
     CDS             complement(1673..2593)
                     /locus_tag="VGR22_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2127059.1"
                     /translation="MGGLTNEIREPAVEVVPLTPADLPALGSTGNPRIDVADMRAMLE
                     AAPGRSFWVPDTAEFILVQAWRNREELPSIHTLWSFTGDDALIRAAADAAQRLDAAAL
                     VMLETGERRSPTFYHRHGFVRIETIRTYEHVEPEVLARQIVPGSQSFSEVTLNRPDLL
                     TAVIEIDHHAFPWFWWNSEAEFDTYLQQPGVEVWAGLRDGEVVSYIGITSFHHWAHLD
                     RIAVDPMRQGQGLGRSAVTFAAERMLGAGARRIGLSTQGSNRVSRHLYESLGFQHTRQ
                     SDYDVFGIVFDADRVYRAARRVLSSDESGS"
     gene            2740..3408
                     /locus_tag="VGR22_00400"
     CDS             2740..3408
                     /locus_tag="VGR22_00400"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEV2127060.1"
                     /translation="MDQPSLPIFPLGTVLFPHGLMPLHIFEERYRRMLRDTSHNDPAF
                     VVALAARNEPDITRLEPETIGTACRVSGISPRPDGRSDIAVTGTTRVRIGVDAIDWSN
                     GYAVAPVEVLNDLQEGPEEVARRYATIRARFHRYLHALERLIGERLPALEPSRIPEKG
                     SWVIAETLTLHTWERQLLLEQPTIDARLEVLEWFLKREHALLVQTGMIGSPIDFPGRN
                     LTMN"
     gene            complement(3541..3918)
                     /locus_tag="VGR22_00405"
     CDS             complement(3541..3918)
                     /locus_tag="VGR22_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127061.1"
                     /translation="MDTTHAPDPRRIVVIGPCGSGKTTLSHRLRALGFDAHACGQEHS
                     EIPELWAHQEPEVLVGLRIDLKTLRRRRSASWSDRLYAKQLERLRSGYDHADLVIDAD
                     VNDEDAVLDAVLSWLRDHEESGL"
     gene            complement(3963..4682)
                     /locus_tag="VGR22_00410"
     CDS             complement(3963..4682)
                     /locus_tag="VGR22_00410"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEV2127062.1"
                     /translation="MAQPVMLDLGLAKTEKYASRDSGDTVEIVERPGGGFSVVMIDGQ
                     GSGQAAKTLSLLLSSKAVGLIKEGVRDGAVARATHDHLFMYRHGRVSATLDLLSVDMK
                     TRSVVVTRNADAPMLMGQGGEYRTVDVGSGPIGRYHFTKPDIREMPLDTGLTIVLFTD
                     GIPQSGRRARSDPIDYIGFAVNHFDDHLPAQEMADRLLEHAVERDDGRPVDDMAVVAL
                     RISDHHEPRYIRRMQAQFPML"
     gene            4814..5575
                     /locus_tag="VGR22_00415"
     CDS             4814..5575
                     /locus_tag="VGR22_00415"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV2127063.1"
                     /translation="MQIATFDFHNTVAYCDPWFQLEIRELPGEVLELLALRTGISVTA
                     DLRDEATGRYRALRQTIMESGVEVDALESVQRIFRDMDLPVDRPALTQAIDELMRATL
                     SDAEPVPGSAETIVSLVDSGVPVGIISSAVYHPFLEWVLARFGLLERLAFVATSASVG
                     YYKSDARIYRHAYERVNATTELGVHVGDSPRWDVDTAKQAGLAAVLYDHPGVKRQTPS
                     PDGLAPPDLVLDSLIGADRPILDLLAARRNGKAGR"
     gene            5572..6291
                     /gene="trmD"
                     /locus_tag="VGR22_00420"
     CDS             5572..6291
                     /gene="trmD"
                     /locus_tag="VGR22_00420"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEV2127064.1"
                     /translation="MTEHQTPFRIDIFTLFPPMFEGPMRESILKRAAAAGLLEIQVYD
                     IRDWTHDRHRTADDTPYGGGAGMVMKAPPIVEAVEDVLGNEIGETHIAIMSAGGRRYT
                     QGIALELSRKRRLALVCGHYEGIDERVGQLLDADELSIGDYVLTGGELAAMVVADAVS
                     RLLPGVIDEESIREESHSVEGVEYPHYTRPVEYRGLKVPDVLLSGHHANIAAWRKEEA
                     RRRTARWRPDLLEYLDDEDER"
     gene            complement(6511..7293)
                     /gene="recO"
                     /locus_tag="VGR22_00425"
     CDS             complement(6511..7293)
                     /gene="recO"
                     /locus_tag="VGR22_00425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEV2127065.1"
                     /translation="MADSLRDRLYSTEVIVLSRRNLGEADRIYDVYSVRHGRMSMIAK
                     GARKAENRNGRTLDLLNRVVVQLYRGRNLDVVRGVEPVATHPGLRSDLEAFGHASYIA
                     ELIRVMTHDHEPSAQIYTLLAETLALLADGVDPWPLTRYFEYALLEAAGFQAQLYECP
                     RCREALTAQVNAFSMREGGVVCPRCRVDDPGSTPLSVNAQKYLRTMQREGLRRVLTLK
                     LDSESRLQIQQALTGYMQHLAERPLGSLAVLHALQGRRDVVS"
     gene            7456..8202
                     /locus_tag="VGR22_00430"
     CDS             7456..8202
                     /locus_tag="VGR22_00430"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEV2127066.1"
                     /translation="MTEVTFLTALIAGVFSITSPCMLPLIPIYLTHIAGVSAGEPGVR
                     ARALVLRNAAAYVIGFSVVFIVLGAALGTAGALAGSLDFISENRLWLVRIGGVLLLIL
                     GLHQLGWITVPFLNRTRRANLQAENPGTLLSSFLIGVTFGAGWSPCVGPILGAILTMA
                     AGQGSIGRATTLLTVYSLGLAVPFMLAALAFGSAPGIIRKLNSWMAMVTTISGAVMIG
                     VGAIMILGVYEQIFIELIRVAPWTPYEPEI"
BASE COUNT         1413 a         2820 c         2567 g         1444 t
ORIGIN      
        1 gtcacctgcg agcccagcgg aaagccctcg ggcacagacc acgtcgtacc ggagtccgtc
       61 gaaagcagca ggccggagca gtcatgcgca actgcgatac cggtgttcat ccacgacgcc
      121 agcggtaccg cgcccgccgg gcccgcctcc acccacgtcg cgccgccatc cgtcgatcgc
      181 accatcggcg ccggcgcctc aaccccgcgg tagcagtcca ccggcgtccc ggcccagacg
      241 agcagatcat cgcgcgaatc gacgaccatc acgtggtgac ccggcgccac acctgtccga
      301 cgccagccgc cggtctcgtc atgcgtccag gtggcaccac ccgtgccgag gtaccgggcc
      361 tcgccactga ccggctcgac tcccagtgcc gtcactggct cgccctcgac ggtgagcggc
      421 gagacgctgg tttgcccaac tccgacgcgc gacacggcct cgaccggtgc catgaacgcg
      481 ccgaggccga agaggatggc aagaatcagg gtcaacagcc acatggccta cctcccatga
      541 cacagtcggc attcactttg ctgaaacgcc gggcgcgtcg cacccgtttc attcaatcag
      601 tcgggccagt cgatccgcga cgcccggttg cgcaggtaga gatccgccag cacgtttgcc
      661 aacatcgcct ccaccaccgg cacggcgcgg ggcaccaccg atggatcgtg ccggccctcg
      721 accttcacgg tcgtcggctg cagctcggtc gtcacggtgg actggtcctg ctcgatcgac
      781 gaagttggct tgatcgcgac ccgcacgacg atctcctcgc cggtcgagat gccggcgagc
      841 gtgccgcccg cgttgttgct ctccgtacgc acccgaccat cgcgaatccc gaaaggatcg
      901 ttcgactccc ggcccgtgag acgcgcctgc tcgaaccccc gcccgatctc gacgcccttg
      961 gttgccggga tgctgagcat tgcctgcccg atcagcgcgt ccagcttgtc gaaggtcggc
     1021 tcgccgagtc ccggtggcac tccgatcgcg cgagcctcca ccacgccacc gaggctgttc
     1081 ttctcttcct tgaccttcat gatcgcgtcg atcatcttct cggctgcgac cgggtcgggg
     1141 cagcgcacga tgttgcgctc gatctccgcc tcgtcgaagg tctccatctc gatgcccccg
     1201 atctcgcgcg tgaagccgaa aacatgcgcg ccagcgtgcg cgaggatctt ccgtgcgatc
     1261 gcgccggcag cgacccggcc ccacgtctcc cgcgcggacg accggccacc gccgcggtgg
     1321 tcgcgatgcc cgtacttcat ttggtaggtg tagtcaccat gccccggacg gaagagattg
     1381 cgcaggttct cgtacttgga ggaatcgaca tccgcgttgt aggtaatgat cgaaatcggc
     1441 gtgccggtcg tcactccctc aaagacgccg gagaggatct cggcacggtc cttctcgttg
     1501 cgcggcgtcg tcaccttgct ctggccaacc cgccgccgat ccagttcgtg ctggatgtct
     1561 tcgacggtga gcggaatccc ggcaggacac ccctcgacca ccacgcccag cgcgggaccg
     1621 tgtgactcgc cccacgtcgt caagctgaaa atcgtcccga acctgctgcc catcaactcc
     1681 cgctttcatc cgaactcaac acacgtctcg cggcccggta gacccgatcg gcgtcgaaga
     1741 cgataccgaa gacatcatag tcggactggc gggtgtgctg gaacccaagc gactcgtaca
     1801 ggtgacgcga tacccggttg ctcccctgcg tactcagacc gattcggcgc gcccctgccc
     1861 ccagcatgcg ctcggcggcg aacgtgacag ccgatcgccc cagtccctgc ccttgtcgca
     1921 tgggatcgac cgcgatgcgg tcgaggtggg cccagtggtg gaagctggtg atgccgatgt
     1981 aggagaccac ttcgccatcc cgcaaccccg cccagacctc gacgcccggc tgctgtaggt
     2041 aggtgtcgaa ctccgcctcg gagttccacc agaaccatgg gaaggcatgg tggtcgatct
     2101 cgatcaccgc cgtgaggaga tccggccggt tgagtgtcac ctccgagaaa ctctgactgc
     2161 ccgggacaat ctgtcgcgcc agcacctccg gctccacgtg ctcgtaagtg cgaatggtct
     2221 cgatgcgcac gaacccatgg cgatggtaga aggtcgggct gcggcgctct cccgtctcca
     2281 gcatcaccag tgcggccgcg tccaaccgct gcgccgcgtc agcggctgcc cggatcaggg
     2341 catcgtcacc ggtgaaggac cagagggtgt ggatgctcgg cagctcctcg cgattgcgcc
     2401 atgcctgcac aaggatgaac tctgcggtgt ccggcaccca gaatgaccgc cccggcgcag
     2461 cctccagcat cgcccgcatg tccgccacat ctattcgagg atttccggtg gaaccgagcg
     2521 ccggaaggtc ggcaggtgtc aggggcacaa cctccactgc cggctccctg atctcgttcg
     2581 tcagccctcc caacgatccg tccttccgga gttctgccgt gccggcagcc agcgtccgtt
     2641 cctgcccaag tgtacgaggt cacccgctcc ggtgcgccgt ggaccggggc aaactctata
     2701 cttgaactcg caggcctgca tcgcacgctg gatgctctca tggaccaacc ctcgcttccg
     2761 atctttccac tcggcaccgt gctcttcccg cacggtctca tgccgctcca catcttcgaa
     2821 gagcgctacc gccggatgct gcgcgatacc tcccacaacg accctgcatt cgtggtcgcc
     2881 ctggcggccc gaaacgaacc cgacatcacc cggttggagc cggagacgat cggcaccgcc
     2941 tgcagggtat ccggcatcag cccgcggccc gacggccgct cggacatcgc cgtcaccgga
     3001 acgacgaggg tgcgtatcgg cgtcgatgca atcgactggt cgaacggcta tgctgtcgcg
     3061 cccgtcgagg tgctgaatga tctgcaggaa ggcccggagg aggtcgccag gcggtacgca
     3121 acgatccgtg cccggttcca tcgatatctt cacgcactgg agcggctgat cggcgagcgg
     3181 ctaccggcgc tggagccgtc ccgtatcccg gagaagggaa gctgggtgat cgcggagaca
     3241 ctcaccctgc acacgtggga gcggcaactg ctgctggagc agccgacaat cgacgcgcgg
     3301 ctcgaggtgt tggagtggtt cctgaagcgg gagcacgcgc tgcttgtcca gacgggcatg
     3361 atcggcagcc cgatcgactt ccccggccgc aacctcacga tgaattgaat ccaagacacc
     3421 aagactctcg tgcatgggcc tgccttgttg cgtccgctgc gcgacgccat gactggcgac
     3481 caggcctcag gggaaccgcc acttacgccg tcgagacacg gacagacaca agatctgtcc
     3541 ctacaaaccg ctctcttcgt ggtcgcggag ccagctcagg acagcgtcga gaaccgcgtc
     3601 ctcgtcgttg acgtcagcgt cgattaccag atcggcgtgg tcgtacccgg acctcagccg
     3661 ctccagctgc ttcgcgtaga gccggtcgga ccaactcgcc gacctccgtc tccgcagcgt
     3721 cttcaggtca atccgcaaac cgaccagaac ctccggctcc tggtgtgccc agagctccgg
     3781 tatctcgctg tgctcctgtc cgcacgcgtg cgcatcgaag ccgagcgccc tgagccgatg
     3841 agacagtgtg gtcttgccgg acccgcacgg gccgatcacg acgatgcgcc ggggatcggg
     3901 cgcgtgcgtg gtgtccatga tccacctcct gctccagcga catggcgacc ggaacgtcga
     3961 tactacagca tcgggaactg cgcctgcatg cgcctgatgt atcgcggctc atgatggtcg
     4021 ctgatgcgca gggcaaccac cgccatgtca tcgacaggtc ggccgtcatc tcgctcgacg
     4081 gcatgctcca gaagccggtc ggccatctcc tgtgcgggca gatggtcgtc gaagtggttg
     4141 actgcaaacc cgatatagtc gatgggatcg cttctcgctc gccggcccga ctgtgggatg
     4201 ccatcggtga agagcacgat cgtcaatccg gtgtccagcg gcatctcacg gatgtccggc
     4261 ttggtgaagt gatatcggcc gatcggcccg gaaccgacat cgacggtgcg atactcgcca
     4321 ccctgaccca tcagcatggg cgcgtcggcg ttgcgagtga ccaccaccga gcgggtcttc
     4381 atgtcgaccg agagcaggtc gagcgtggcc gagacccgcc cgtgccggta catgaagagg
     4441 tggtcgtggg tcgcgcgggc gacagcgccg tcacgaaccc cctccttgat gagcccgacg
     4501 gccttgctcg acagcagcag gctgagcgtc tttgcggcct gcccgctccc ctgcccgtcg
     4561 atcatcacca cggagaagcc gccgccgggc cgctcgacga tctcgacggt gtccccgctg
     4621 tcccggctgg catacttctc cgttttcgcg agcccgaggt cgagcatcac cggttgcgcc
     4681 acagtcctct cgttcccttt cgtgccgtcg taccttcacc tgtcccaatc atcccacgac
     4741 acggccgctt ggaacgacag gatgctatcg ttgatgacag gatcgacgat acccgccgtg
     4801 acaggtgcca tccgtgcaga ttgcgacatt cgacttccat aacaccgtcg cctactgcga
     4861 tccctggttt caactggaga ttcgcgaact gcccggcgag gtgctggaat tactggcgct
     4921 ccgaaccggc atctcggtaa ctgcggacct gcgcgacgaa gcaacggggc ggtatcgggc
     4981 gctgcgacag accatcatgg agagcggcgt cgaggtcgat gcgctggagt ccgtccagcg
     5041 catcttccgc gacatggatc tcccggtgga tcgcccggcg ctcacccagg cgattgacga
     5101 gctcatgcgc gcgacgctgt ccgacgccga gccggtgccc gggtccgcag agacgatcgt
     5161 gagcctggtt gactctggcg tgcccgtcgg gatcatctcc agcgccgtct accatccgtt
     5221 tctcgaatgg gtgctggctc gctttggact cctcgagcgg ctggccttcg tcgccacctc
     5281 ggcgagcgtt ggctactaca agtcggatgc ccgcatctac cgccatgcct acgaaagggt
     5341 caacgcgacg accgaactgg gcgtccacgt cggcgactcg ccgaggtggg acgtcgatac
     5401 cgccaagcag gctggcctgg cggcggtgct ctacgaccac ccgggcgtga agcggcagac
     5461 gcccagcccg gacggcctgg cgccacctga cctcgtgctc gactccctga tcggtgccga
     5521 ccgaccgatt ctcgacctgc tggcagcgcg ccgaaacggg aaagccggca gatgaccgaa
     5581 catcagacac cgttccgaat cgacatcttc acgctcttcc cgcccatgtt cgaggggccg
     5641 atgcgagaga gcatcctcaa gcgcgccgcg gcggccggct tgctcgagat ccaggtctat
     5701 gacattcgcg attggaccca tgacaggcat cggaccgcgg acgacacgcc ctacggcggt
     5761 ggcgcgggca tggtcatgaa ggcacctccg atcgtcgaag cggtcgagga cgtgcttggc
     5821 aacgagatcg gcgaaacgca catcgcgatc atgtcggctg gcgggcggcg atatacgcag
     5881 ggcatcgccc ttgagctctc ccgcaagcgc cgcctcgcgc tggtctgtgg gcactatgaa
     5941 ggcatcgacg agcgcgtcgg gcagctgctc gacgccgacg agctgtcgat cggcgactac
     6001 gtgctgacag ggggcgagct cgctgcgatg gtggtcgcgg acgccgtctc ccggctcctc
     6061 cccggcgtca tcgacgaaga gagcatccgc gaggaatcgc acagcgtcga aggcgtcgag
     6121 tacccgcatt acacgcggcc ggtggagtac cggggtctga aggtgcccga cgtgctcctc
     6181 agcggccacc acgcgaatat cgctgcgtgg cggaaggagg aggcgcggag gcgaaccgcc
     6241 cgctggcgcc cggacctgct cgagtacctc gacgatgagg acgaacgcta gagccggggc
     6301 agccctgcca tttcgagcga agcggcgcag ccgcggagtc gagaacgctc ttgtgcgcgt
     6361 tgtcagacac cgattcctga tgctatcgat gggagagact tatccactct gcaagcgtcg
     6421 catgcgagcc tgccctgagc ggagacgaag gggtcgaaat cagccactgg ctgacaggtg
     6481 tccgaaggcg tcggctgcag actgtagctg tcaggagacg acgtcgcggc gaccctgcag
     6541 ggcgtggagg acggccaggc tgccgagcgg acgctcggcg agatgctgca tgtaccccgt
     6601 cagcgcctgc tgaatctgca gccgcgactc cgagtcgagc ttgagggtga gcacgcgccg
     6661 caagccctca cgctgcattg tccgcaggta cttctgcgcg ttcaccgaca ggggggtgct
     6721 gccgggatcg tccactcggc agcgcgggca cacgaccccc ccttcccgca tcgagaaggc
     6781 gttcacctgg gcagtaagcg cttcccggca acgcgggcac tcgtagagct gcgcctggaa
     6841 accggctgcc tccagcagtg cgtattcgaa gtaacgggtg agtggccacg gatccacgcc
     6901 gtctgccagg agcgcgagtg tttccgccag cagcgtgtag atctgcgcgc tcggctcatg
     6961 atcgtgcgtc atcacgcgga tcagctcggc gatgtacgat gcgtgcccga acgcctccag
     7021 atcagatcgc agacctggat gggttgccac gggctccacg ccgcgtacca cgtcgaggtt
     7081 gcgtccccgg tagagttgca ccacgacccg gttgagaagg tcgagcgtcc gcccgtttcg
     7141 gttctccgcc ttgcgcgcgc ccttcgcgat catcgacatg cggccatggc gcacggagta
     7201 gacatcgtag attcggtccg cctcgccgag gtttcggcgc gacaacacga tcacttccgt
     7261 ggagtagagg cggtccctga gggaatcggc catgagcgat gaatccagca tgagcccggc
     7321 ttggaccgga ggtgagtacg gggaaggaaa tcgggggaat caggtcggtg gtacatggca
     7381 gaaatgccgg tgttccggag cattccagtg catcagcatt gtatccggaa tcgccagaat
     7441 ggtagggttc cggggatgac cgaggttacc tttctcacag cgctcatcgc gggtgtcttc
     7501 tccatcacct caccctgcat gctccccctg attcccatct atctcacgca catcgcgggg
     7561 gtgtcggcgg gtgagcccgg tgtgcgagcc cgcgcgcttg tgctgcgcaa cgcggccgcc
     7621 tacgtgatcg gcttctccgt cgtcttcatc gtgctgggtg ccgcgctcgg caccgccgga
     7681 gcgctggccg gctcgctcga cttcatctcc gagaaccggc tctggctcgt gcggatcggc
     7741 ggcgtgctgc tgctcattct cggactgcac cagctcggct ggatcaccgt ccccttcctg
     7801 aaccgaacgc gccgcgccaa cctgcaggcg gagaaccccg gcacgctgct ctcgtcgttc
     7861 ctcatcgggg tcaccttcgg ggccggatgg tcaccgtgcg tcggaccgat cctcggggcg
     7921 atcctgacga tggccgcagg acagggcagc atcgggcggg cgaccacgct cctgaccgtc
     7981 tactcgcttg gcctcgccgt gccgttcatg ctggcggcgc tcgccttcgg cagcgcaccg
     8041 ggtatcatcc ggaagctcaa cagctggatg gcgatggtaa cgaccatcag cggcgcggtg
     8101 atgatcggcg tcggcgcgat tatgattctc ggtgtctacg agcagatctt catcgaactc
     8161 atccgcgtcg caccgtggac gccctacgag cccgagatct gatcatatcc tttcttttca
     8221 catacgcccg gcacggttgg tttc
//
LOCUS       DASYWL010000013        24622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_131816, whole genome shotgun sequence.
ACCESSION   DASYWL010000013 DASYWL010000000
VERSION     DASYWL010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 24622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24622
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131816"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..577)
                     /locus_tag="VGR22_00435"
     CDS             complement(41..577)
                     /locus_tag="VGR22_00435"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEV2127067.1"
                     /translation="MSNALIIVDVQNCFMNSYTEHIPDRVVQLIEDNDYDPILFTRFI
                     NQPGGPYPRFMDWCDCTDAPGTDFVDQLAPLATPETTFDKHGHAGIPETLTDYLREHE
                     IDRITVVGIDTDMCVLKVAMDIFDLNIESIVVIDCCASTAGLQSHLAGLAVLARNIGA
                     DQLREAGLTGGHLAAPRD"
     gene            complement(877..1761)
                     /locus_tag="VGR22_00440"
     CDS             complement(877..1761)
                     /locus_tag="VGR22_00440"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEV2127068.1"
                     /translation="MINDIEPRLRLTRRTLLATAGGSAVLLAIPAAIALAHDDDDHDD
                     RDDDDRDNSGPGNAEDRDDHDDHDNSGPGNAEDRPDRDDDDDDDDDDFDDEGDVAAQG
                     TVPPGSVEVRIVDDDTNGFQPGPITIDPGQQVTFVNLDDDPHTATGAGFDTGIMQPGQ
                     QVTITFDEPGTFPYSCQIHPVMTGVVEVRGEVAATSASPEASPAAATPAAAGGEAAVT
                     IVDFSFDPAEIEVAVGTTVTWTNEDSVPHTATAEDGTFDTGALNQGESGSHTFDTPGE
                     YPYICSFHPNMHGVVIVT"
     gene            complement(1926..3365)
                     /locus_tag="VGR22_00445"
     CDS             complement(1926..3365)
                     /locus_tag="VGR22_00445"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV2127069.1"
                     /translation="MTDPRATDMQHEAVVVGSGPNGLAAGISLAEAGHTVRIYEANDT
                     IGGGCRSLELTLPGFVHDLCSAIHPLAVASPFFRTIPMASYGLEWANPPFAVAHPFDD
                     GSAAVLSQSLEETAASVGEDGQAWIDLFAPLVEDFDILIGPLLGPFQLPRHPIAMARF
                     GLSGLQPAHRMMRSRFSGERARALFAGLAGHSIIDLNKPATAAFGLVLGLIGHVVGWP
                     LPKGGSQQITNALVEHFRALGGEIVTGTPVTSLDIADGADAVMFDVTPKQFLGIVGDA
                     VPGRYRRQLEQYRYGGGVFKIDWALDGPVPWTAEECAQAGTVHLGATMEEIVTAEHEA
                     AIGRHPERPFVILAQQSLFDETRAPDGKHTLWGYCHVPNGSIVDMTERIEAQIERFAP
                     EFRECIIERSVIPPAEMEVHNANYIGGDINGGIQDIRQLFTRPVPRLDPYSTPNPRLY
                     FCSASTPPGGGVHGMGGYHGARSALRRAW"
     gene            complement(3362..4129)
                     /locus_tag="VGR22_00450"
     CDS             complement(3362..4129)
                     /locus_tag="VGR22_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127070.1"
                     /translation="MVQPGDVRLQHDADKDKAVGAKHHSFSPDPLPLDSLDLNVEGRD
                     LAPPQEGFGQLWRKRFRIRLTGANVPSTEVVRVWREHFGEFWPESNRFYRPLGGLKPG
                     EVALADLEMAPGGRLSTGVVVVDASPTSFTFMTPRGHSFAGQITFSAHDEAGTTIAQV
                     EILMRASDPLFEISLLLGGHKRENEFWQTTLLELADHFGVEDQEPEVTITCPDPNRQW
                     SKATNIVYNSLLHTAFYMATRPLRRLLERLKRRGETS"
     gene            4423..4926
                     /locus_tag="VGR22_00455"
     CDS             4423..4926
                     /locus_tag="VGR22_00455"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEV2127071.1"
                     /translation="MLRSPLFNDFLTLRNSIDQLAEGVFGGDNYRTLWSRAGTGNGNS
                     VYPMPLDVYATDDQAVIVAAVPGMHPDDLDLTIQQNTVTLSGSVRSVTDSEEAKGATW
                     YIHELASGTWRRSVTLPFQVDADQAEATFEHGILRVVLPKAENARPKKIAISGSQHEA
                     IGTGASS"
     gene            complement(5331..7484)
                     /locus_tag="VGR22_00460"
     CDS             complement(5331..7484)
                     /locus_tag="VGR22_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127072.1"
                     /translation="MRLLHSPTSNRWRWPVTLALLTLLYLVLRVTDLDQLVTVDEPFW
                     LARSANFYRALWQGDLAYTFQHAHPGVTTMWAGMVGYWVAAPGYVDAFDTNLDNVFNI
                     HQRLREMGLAALDVLIAARLAKIGLQAMLFLLSLVLLRRLFGVGITVVAGAIIALDPF
                     LIAHDQLLQIDGLVTISSLAAALALADAVTRDRWDAAPWIVAGALAAIAWLTRITGLA
                     LIGVTGLVLLITVLMGRRGTLGARLRTATRYGTLWLTAALITTIALWPALWTGPERAV
                     AFMRRYVAGAVAEGHEGPIYFMGDLHTGDPGVLFYPVSMLWRLSVASSIGLVLAAVSL
                     AVPSIRRALPARLLRAIGILLLFAVLYLVGMALGAKKFDRYVLPVHAVLDVIAAIGIV
                     ATLRWALPRRRSIARVAMPIVLIALLLAQTLVTLGHRPYYLVAYNPLLGGAEAAEDVV
                     RMGYGEGTDAAARWIIEDSGVRPGQEIDDPPVVHISGAPAPLHYLLPPPFMVDPGGFR
                     TAEDWEVTDYYVVSIQQRRVREWPPNQYGPTIAYLQRFEPAHTVWIEGVRYADVWDLS
                     EIPSPPWLTGPASCEWQFEPWLQLENVVIDGEIIALWFQTLTAVDLPGRVVVTVDLVP
                     RTLNTPAVDQTWEATFEPRPRRGLVTDVMLEIPPLAAEATGDYLLEITVHDGETGESL
                     PAYPPNSSISHPAAVIGSRCASGVPDANPANVPSA"
     gene            7947..8924
                     /locus_tag="VGR22_00465"
     CDS             7947..8924
                     /locus_tag="VGR22_00465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEV2127073.1"
                     /translation="MRSLMVRLAMLFGVLAMISAPAALAQERMSIATGGTGGVYYPYG
                     GGLADLLSEQLEGYEFTAEVTSASVDNMYLIDQGDADLAFVLSDTAYDAVQGNEPFED
                     PINARSLVTLYNNFTQVVVNPESGIESLEDLAGKTVSTGSPGSGTEVIAQRLLEVAGL
                     STDDFQQEQLGASESAAAFKDGKIDAFFWSGGLPTSAITDLGATPNISYELLSTDEYV
                     EEMQEQFGQFYDVDTIPADTYPDQSEAVETIVVPNVLVVHADMDEQLAYDIVRTMFEH
                     KDELVAVHSAANNLQLETAMSSTALEYHPGAVRYFEEQGVDIPEATPAS"
     gene            8968..9471
                     /locus_tag="VGR22_00470"
     CDS             8968..9471
                     /locus_tag="VGR22_00470"
                     /inference="COORDINATES: protein motif:HMM:NF020479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
                     /protein_id="HEV2127074.1"
                     /translation="MLIRRMLWLPAATLVAMALLGIALQPATRLTVTTDDGRVLVCEQ
                     MAPGDTVTLVFTHSMYGVEVRESWRVVNGRLVRTGIETDRAAAAEYYATDGAVERTDG
                     GFAVISPPLTVDALVVRVDQIGRHRLRSDGTDEISLADRVEGSAAVTIQTRHQALLQQ
                     GIAGCRG"
     gene            9507..11693
                     /locus_tag="VGR22_00475"
     CDS             9507..11693
                     /locus_tag="VGR22_00475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HEV2127075.1"
                     /translation="MTDRDKDREAQHVDPQTEEAQAESNIELKEVNVADFEAESPTRQ
                     LSGPMTVIVAVIAIGLSVYALYATRTTIPAQTYRTIFLAIVLVLTFLLYPARRRNAPL
                     VPILDVVTIMVAVAVLGYPLVDNSMVLMALLAVAVPLVFIVVPGLAGGNGFSTRSLFA
                     VIPDGALVVLSVIAALYPLWDFDNFIYRAARPNETDVLMGVLLILLVVEATRRTVGWI
                     LPAVAVGLIAYGRFGDMLSGAWGHRGYDVDRMVGTLYISLEGIYGVPLDVAATYIILF
                     TIYGAVLEFSGAGKFFLDFSFAATGRRASGAGRTTTIAGFLLGTVSGSGVATTVTLGS
                     VAWPMLRKAGYDPESGGAVLSAAGIGAILSPPTLGAAAFLIAEFLQISYLEVIKMAMV
                     PTILYYLCIFLMIELDARRMGTRAVIIETLPLKTLTMRYWYHFTSLIAIVVLMIMGFT
                     PITAVFWAIVLAFLLSFLRRETALTPIRTAQALRGGAVGTLSVAATTATAGIIVGIMV
                     LTGLGLKMSDLIVDLANNNLFLTVLFTGIAVWVLGLAVPVTASYIIAAAITAPALTTL
                     GVPDVAAHMFIFYYAVLSEVSPPTALSPFAAAAITGANPFKTMMVTWKYTLPAFIVPF
                     MFTLNTESGVHLLAIGDVERIILATVTACLAIVALVAGVGGWVVQRANMVERAILIVA
                     AGLLLYTGPVQDAVGLTLMVIGIGVHWLRVRGQGSDTGGEHPVAVA"
     gene            11798..12334
                     /locus_tag="VGR22_00480"
     CDS             11798..12334
                     /locus_tag="VGR22_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127076.1"
                     /translation="MHDPSQNRILVHRIQQAVSRGVAKPIEEELMSLYRVPIESTSYD
                     NRGKPAFGLAETALVIAVLWPLVHKFFEPLVTTAGEAFRNRVLETIRKTRHTPSGPHI
                     SVAIQFGDESDEEFPDTPIRYYFYHQIDDEELVKRLKAADKHLKSLPCELFSERSAPV
                     EYGFFWDRETQQWRGCVW"
     gene            complement(12574..12876)
                     /locus_tag="VGR22_00485"
     CDS             complement(12574..12876)
                     /locus_tag="VGR22_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127077.1"
                     /translation="MTGSIRPTITEDHMTISSTSHRPSAAEGLHTYGLAGIDLQRATE
                     RRTTATSARTTDRQSSLLHRMRQAAGDAIIRLGASIAGEAARPTTAPSIQPKVDLA"
     gene            complement(12966..15314)
                     /locus_tag="VGR22_00490"
     CDS             complement(12966..15314)
                     /locus_tag="VGR22_00490"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEV2127078.1"
                     /translation="MQAIALHHQHIPLPATSLVGREGDLARITEVLSRPDTRLVTLIG
                     PGGVGKTRLALQIAHDLDPRIAEEVALVLLTPVDEAAEVLPAIAHGIGISQSDTLPIE
                     DRIISAVDSRRLLLILDNAEHVAEHLALLGQLLARCPNLKILVTSQMMLRLSAERVVP
                     IEPLPVTSTSISELAPATELFVERARAVRLDLEVTRETVRAIDDICQQLDGLPLAIEL
                     AAARARFLSPTALRDRLSERLPVLVGGPRDAPERHQTLRATLHWSHDLLDDDERALFR
                     RLAIFRGSAPFDAVEPVCNADGALGHRTEELLGVLADHSLVRIFEPPDTGPRVRMLNT
                     IHEFAQEQLEVSGEANRIAGVHAAWFAQAVIDQPEEHWRTGTSELRVWTVRYERDSDN
                     LQVALERTVAQEDWTRAVQMVSALVSFWMETGQIRETRVWTQRLAPYADAMPPEIQLR
                     FWYMAAIAAFANEVPDDALASARRALTLADELGHARLAANAQNLIGMIHWQRGEAEEA
                     ERMQLLAVETIHKAPDRTRGGMFLATIGEQLIEHGQAERGEALLREGLEAIEHDRPEV
                     KALALGALVDLLLARNDIDEAGETLEASLAYHRDPPHRQPVALADRLCSAARLATLCD
                     RPVDGARLRGAASAILERLGMMQHSQTEEMLARTDARLRAVLDEVTLRREMNAGRGMS
                     IPEAIALATDIARMRSEAPSQAEPPPAERKDAGLTDRQREILRLLAAGKSNAAIAEEL
                     FISERTVTTHLTRIYDRLGVATRGEAIARAHELGIVPAASRT"
     gene            15421..16035
                     /locus_tag="VGR22_00495"
     CDS             15421..16035
                     /locus_tag="VGR22_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127079.1"
                     /translation="MPDITVKPDVAFAGDNPLLMLYRPDTEEVIAVASYWRCAWSPEG
                     AGEALVIWVDPAASGLDEQAPAGIFADNTAMAHLVWVQFNSQFDRLQGRGIEDREIQP
                     ARFTMLADGIRLHRVTCAAGATTIELEWRDALEAVHMVATPRVGSSRWEVTNVVLPCA
                     GAAIRVDGIEIAGEVHQPEGIYRSSAFLAFAESWVRLADAGGES"
     gene            16310..16828
                     /locus_tag="VGR22_00500"
     CDS             16310..16828
                     /locus_tag="VGR22_00500"
                     /inference="COORDINATES: protein motif:HMM:NF018765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1360 domain-containing protein"
                     /protein_id="HEV2127080.1"
                     /translation="MDGSGNEQQREQKQPENAAYITLIGIFLGLFAAFSKREQENESQ
                     LRLTGLDLTMLGLASFRTGRLTAYDQVTKPLREPVTKTESDSYGTSQTTAPEGSGVRK
                     ALGELVACPTCTGTWVAAFLVYGLRVAPGPTRLFLAFMSATGLAELLDSANEALFWTG
                     RAERKQAKPPTS"
     gene            16877..18253
                     /locus_tag="VGR22_00505"
     CDS             16877..18253
                     /locus_tag="VGR22_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127081.1"
                     /translation="MVRLLLTHGRGLSGRNQEYLRRKFTRLVQEGLRNVGYDDLTSEQ
                     VDFLYYGDLLDRAEREYLPEPTPLIEIGRREGAEVSRYDFKDDLEHLHRHLGALAVAD
                     AGICNIVEERAGQVPIVSEASDEASRRAAIEIALEDRTAQLLENPNELLDVLERGFGP
                     AYGDDEAVRRHLHELDERLGDDPRLIAARRGHEEEGTDDTNRVEDLLREWILGPVTDA
                     IASLQAVSRIIGDYGIDLRKRLGEWRLAAWSEAQTRFEGTDFGRQWALLFDIIAAVAH
                     LTMLDGVYIARRMADVEIYFRDPSIRNDVRIAFRNAVLGADGATILVAHSLGSVIGYD
                     VLREYERLQVPGLVTVGSPLSIGHFRRNLARKGESGDNLPVPSMLEGWVNVYSEMDPL
                     VLGSGIERWFQGGGDESGSAPVDREAGNTGYLDAHSPDQYLRSKETASAIVRMVTRAM
                     RDVRSSNW"
     gene            complement(18954..20564)
                     /locus_tag="VGR22_00510"
     CDS             complement(18954..20564)
                     /locus_tag="VGR22_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127082.1"
                     /translation="MCPLPQLRAAAVTLTLLIAIAAMLPALTAAQTPGTDGASLQGCA
                     APGTVSVVAGAAGGKAVTIAETETGITLTMPDGTTHDLPAGELRLEPGMAIVLADDGT
                     HMVLSTATGETWLFDPVEYRDFQAGMTPATAPFVQTGPYVAAPITDDGLDWQIVDMRT
                     GESATTSEILGGPFPVAADAYALPNSGDDRGIAAIRFRQMAEPIAGLGATPTPFANVG
                     ATLVIPGDLAGAAFVDLEPEALLFHDADPMLFAGNRSVVFSPDHSQVAWATGSGDPFG
                     ETHEVLVASLDALEEPTAYLAPGAPDARVPLLFTPDGTALLALVDGALTRIDLATGEA
                     QPANAPGITGEGIRVANPVDGTIIYDAGTPEQPEVMLYDAATDTATPLPELNAYPWIY
                     PLAGWTYAGATGAGDPASTVLIDTRTGQIATDLPTAGDNQTVLSEAMLAGDFRDGVLV
                     TGRPDGTVLVQDGATGEAWVTPMPEGMEPGLVPRLSISPDARCLLLSTTTAGSNMPFA
                     SWLAPIAAGATWTALETPALGWWQIGGE"
     gene            20685..21578
                     /locus_tag="VGR22_00515"
     CDS             20685..21578
                     /locus_tag="VGR22_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEV2127083.1"
                     /translation="MTDERPDSSPPHQPPDPVREPRPPDQAEEREEKHRERWELLDNL
                     QALLEPAMTVLGLVFLVLLLVDYANTELGPLGRVRIDQALQVIWGVFVIDFAVRLWVA
                     PAKLAFLRANWLTIISLALPFLRPLRAFRAIRAVRSLSLVRLLGGMNRGMRVIRRITR
                     GAQLAYVGLLTLVVMLAGAVGAYFFEQGVADAQIRTLGDAIWWSSALVTTINNEKYVV
                     SPEARTIAILMRIYAVSIFGYITASIATYLIGSAVSGEGEALESENTALRAEIASLRE
                     EIRAVRRSLERDDNAQDHLQE"
     gene            complement(21909..22664)
                     /locus_tag="VGR22_00520"
     CDS             complement(21909..22664)
                     /locus_tag="VGR22_00520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV2127084.1"
                     /translation="MIDLDSRIVLVTGAGGAIGSATAHTIVQAGGRVVLHDRDKASLK
                     DLAEVLGDRATALVANLVDAEATDDLWARALAAYGHIDVLVNNAGIYPPAPIDDPLED
                     WVAVWDRSLGVNLIAPAVLCRAAVRSFRAQREGGIIVNLASRAAFRGEDPDYWHYAAA
                     KAGVVAMTRTIARQYGREGVTAFAVAPGYVDTPFNQVFAETVGVEVAAADTGLGEVAQ
                     PQDVANVVVFLASGLARHATGATVDVNGASYVH"
     gene            complement(22713..22868)
                     /locus_tag="VGR22_00525"
     CDS             complement(22713..22868)
                     /locus_tag="VGR22_00525"
                     /inference="COORDINATES: protein motif:HMM:NF033488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEV2127085.1"
                     /translation="MLGILWTILVIAVVVWLLGLLLDIGGSLIHVLLIIAAVILIYNM
                     ITGRRAV"
     gene            complement(23011..23253)
                     /locus_tag="VGR22_00530"
     CDS             complement(23011..23253)
                     /locus_tag="VGR22_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127086.1"
                     /translation="MLAPMFHERELIVIGSSDRPDYAAHARWYFPIVHGTGHELERLF
                     DLRISAAELPDTFAPLAAGSTTAKVLVRYENGELGW"
     gene            complement(23376..23975)
                     /locus_tag="VGR22_00535"
     CDS             complement(23376..23975)
                     /locus_tag="VGR22_00535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2127087.1"
                     /translation="MRPPRPRSAPPSTTPRPPRTSTRAGPSRSTATACAVSNHRRPED
                     ATSRTFERVLRSLPDYRVDVTRPGATFRAWLFAIAHNTVVDHHRRRRFHLSLDRLLHP
                     LRNRPAFDPPDPDRAPDDAAISRDEGRAVWIALGHLPERQRRVVELRLAGLTGPEIAT
                     AQNMTLPAVKSVQFRAYQTMREVVTELGYAPDARRTPGG"
     gene            complement(24198..>24622)
                     /locus_tag="VGR22_00540"
     CDS             complement(24198..>24622)
                     /locus_tag="VGR22_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127088.1"
                     /translation="NTHLDHISEPARVRGAQVILEVLEGWSPQTPIIILGDFNSSPYR
                     PTSHAAYTPRTFGLFAEAGFMDAYRSVAGIWPPPASFHNYEGERYVPDRYGTWYIDWP
                     LTRNLRVLAAEILRHRSEDAPVSDHYPVEAVVTYTDVA"
BASE COUNT         4009 a         7733 c         8612 g         4268 t
ORIGIN      
        1 gccgtcagct ggggtttcgt ggtgggtcac tgacggcccg ctagtcgcgg ggagcggcga
       61 gatgtccgcc ggtgagaccg gcttcccgca gctggtcggc gccgatgttt cgcgccagca
      121 ccgccagccc ggccagatgc gactgcagac ccgccgtgct ggcgcagcag tcgatcacca
      181 cgatcgactc gatgttgagg tcgaagatat ccatcgccac cttcagcacg cacatgtcgg
      241 tgtcgatgcc gacgaccgtg atgcgatcga tctcgtgctc gcgcaggtag tcggtcagcg
      301 tctcggggat gcccgcatgg ccgtgtttgt cgaaggtcgt ctccggagtt gccagcggcg
      361 ctagctggtc gacgaaatcc gtcccgggtg cgtcggtgca gtcgcaccag tccatgaagc
      421 gcgggtaggg gccgcccggc tggttgatga agcgcgtgaa caggatcggg tcatagtcgt
      481 tgtcctcgat cagctgcacc acgcgatcgg ggatgtgttc ggtgtaactg ttcatgaagc
      541 agttctgcac gtcaacgatg atcagtgcgt tgctcatgtt cgcttcgatc tccgcagtat
      601 ccggggcagc gcatactccc gctcgactca tatcgggcag ggtaccgcag gtttggggcc
      661 gcgcatcagc ttcctgtccc agcaacggcg ggtcctccgg catcaggagc gcgccgatgt
      721 gcgtgaaaac cgcctggccg catgagcgat tcggtgaagt cccggattcc tgcctcccac
      781 cttactatgg attcaatact atggattcaa tggcacgggc agaccagttc ggcctgcccg
      841 gcgacgaggt gacgcattgg agcatgcccg ggccgatcag gtcacgatca ccacgccgtg
      901 catgttcgga tggaacgagc agatgtacgg gtactcgccg ggcgtgtcga aagtatggct
      961 gccgctctcg ccctggttga gcgcgccggt gtcgaaggtg ccgtcctcgg cggtggccgt
     1021 gtgcggaaca ctgtcctcgt tcgtccaggt gacggtcgtg ccgactgcga cctcgatctc
     1081 cgcagggtcg aagctgaagt cgacgatcgt taccgccgcc tcgccgcccg ccgctgccgg
     1141 ggtggcggcc gcagggctcg cctcgggcga cgcggatgtc gctgccacct cgcccctgac
     1201 ctccacgacc ccggtcatca ccgggtggat ctggcaggag taggggaagg tgcccggctc
     1261 gtcgaaggtg atcgtcacct gctgaccggg ctgcatgatg cctgtgtcga acccggcgcc
     1321 ggtggccgtg tgcgggtcgt cgtcgagatt gacgaaggtg acctgctggc cgggatcgat
     1381 cgtgatgggc cccggctgga agccgtttgt gtcgtcgtcg acgatgcgga cctcgaccga
     1441 tccgggcggc acggtgccct gcgccgcgac atcgccctcg tcatcgaaat cgtcgtcatc
     1501 gtcatcgtca tcgtcgtcgc gatcggggcg atcctcggca ttgccgggac cgctattgtc
     1561 atggtcgtca tggtcgtcgc gatcctcggc gttgcccggg ccgctgttgt cgcgatcgtc
     1621 gtcatcacgg tcgtcgtgat cgtcatcgtc gtgagcgagg gcaatggctg ccgggatggc
     1681 gagcagcacg gcggagccgc cggctgtggc gagcagggtg cggcgggtga ggcggaggcg
     1741 tggctcgatg tcgtttatca tggggcgatc ctttgcgtgg tgaggcgaag ctcccggtat
     1801 gtgggccaga tgcgtcagtc tgtcgcatcg attccggcat ccaatgtaga tcgctcgggt
     1861 ctgggtacga agagggttga cctcccgcag agagtcggcc cctgcatcgg gtaggtatgc
     1921 cgtggttacc aggcgcgcct gagggcagat cgggcgccgt ggtagcctcc catgccgtga
     1981 actccgccgc ccggcggcgt gctcgccgag cagaagtaga gccgcgggtt cggggtcgag
     2041 taggggtcga ggcggggaac cgggcgcgtg aagagctgcc ggatatcctg gatgccgccg
     2101 ttgatatcgc ccccgatata gttcgcgttg tgcacctcca tctccgccgg tgggatcacc
     2161 gatcgctcga tgatgcactc gcggaactcc ggcgcgaacc gctcgatctg cgcctcgatg
     2221 cgttcggtca tgtccacgat cgagccgttc ggcacgtggc agtagcccca cagcgtgtgc
     2281 ttgccgtccg gcgcgcgcgt ctcgtcgaac aggctctgct gggcgaggat cacgaacggt
     2341 cgctccgggt gccgcccgat cgccgcctcg tgctcggcgg tgacgatctc ctccatcgtc
     2401 gcgcctaggt gtaccgtacc cgcctgcgcg cactcctccg ccgtccatgg caccgggccg
     2461 tcaagtgccc agtcgatctt gaacacgccg cccccgtagc ggtattgctc gagctgccgg
     2521 cggtagcgcc caggcactgc atccccgacg atgccgagaa actgcttcgg tgtcacgtcg
     2581 aacatcaccg cgtcggcgcc gtcggcaatg tcgagggagg tgaccggcgt tccggtcacg
     2641 atctcgccgc cgagtgcccg gaaatgctcg accagcgcgt tggtgatctg ctgcgagccg
     2701 ccctttggaa gcggccagcc cacgacgtgc ccgatcaggc ccaacaccag cccgaacgct
     2761 gcggtggccg gcttgttgag atcgatgatc gagtggcccg ccaggccagc gaagagcgcc
     2821 cgcgcgcgct cacccgagaa tcgcgacctc atcatcctgt gtgccggctg cagccccgag
     2881 agtccgaacc gtgccatcgc gatggggtgc cgcgggagct ggaacgggcc cagcagggga
     2941 ccgatcagga tatcgaaatc ctcgaccagc ggggcgaaca ggtcgatcca tgcctgcccg
     3001 tcctcgccca cgctcgcggc ggtttcctcc agcgattgcg aaaggaccgc ggcgctgccg
     3061 tcgtcgaagg ggtgggccac ggcgaagggc gggttggccc actccagacc gtaggacgcc
     3121 atcggaatgg tgcggaagaa cggtgacgcc acggcgagcg ggtggatcgc cgagcagagg
     3181 tcgtgcacga atccgggcag ggtcagctcc agggaccggc agccaccgcc gatcgtgtcg
     3241 ttcgcctcgt agatgcgcac cgtgtgtccg gcctccgcca ggctgatgcc cgcggcgagc
     3301 ccgttggggc cggagcccac taccaccgcc tcgtgctgca tgtcggttgc ccgtggatcc
     3361 gtcacgatgt ctcgcctctt ctcttgagcc gttcgagcaa gcggcggagc ggtcgcgttg
     3421 ccatgtagaa cgccgtgtgc aggagggagt tgtagacgat gttggtggcc ttgctccact
     3481 gccggttcgg atcggggcag gtaatggtca cctccggttc ctggtcctca accccgaaat
     3541 ggtcggccaa ctccagcagc gtggtctgcc agaactcatt ctcgcgcttg tggccgccga
     3601 gcagcaggct gatctcgaag agcgggtcgc tggcccgcat caggatctcg acctgggcga
     3661 tggtggttcc cgcctcgtcg tgtgccgaga aggtgatctg tccggcgaag gagtgtcccc
     3721 gcggcgtcat gaaggtgaag gacgtcgggc tcgcgtcgac gaccacgacg ccggtggaga
     3781 gccgaccgcc gggggccatc tcgagatccg ccagcgcgac ctcgcccggt ttcagcccgc
     3841 ccaagggccg gtaaaaccgg ttgctttcgg gccagaactc gccgaagtgc tcccgccaga
     3901 cccggacgac ctctgtggac ggcacgttcg cgccggtaag ccggatccga aagcgtttgc
     3961 gccagagctg gccgaacccc tcctgtggcg gggccaggtc gcgcccttcg acgttgaggt
     4021 cgagcgagtc cagtggcagc gggtcgggcg agaacgaatg gtgcttcgcg cctaccgcct
     4081 tgtccttatc tgcatcgtgc tgcagccgga cgtcgccggg ctgaaccata catctctctc
     4141 cggacctgtg ggcggtcgcc agtcgccaaa cgctgtcgtg cgccgtctag gccacgagca
     4201 cgaccggagt atcgcaagga gtgggccttc ggcgtctgtg cgattcagac cggttcggca
     4261 aagggggcca agagttgata cgtgtagtga agactacttg acaaactcaa gggtcatttc
     4321 tatcatgatg gcagacggtt tggcgtcacg gccaccgtcg tttggttcgg attgatcgaa
     4381 cggtttcgtt cgattcggat tgagcgatgg aggtgatgaa cgatgcttcg ttctccgctc
     4441 ttcaatgatt tcctgaccct tcgcaacagc atcgaccagc tcgccgaggg tgtcttcggc
     4501 ggcgacaact accgcacgct ctggtcgcgg gccggcacgg gcaacggcaa ttccgtctac
     4561 ccgatgccgc tggacgtcta cgcgactgat gaccaggcgg tgatcgtcgc ggcggtcccg
     4621 ggcatgcatc cggatgacct cgacctgacc attcagcaga acacggtgac cctgagcggc
     4681 tcggtgcgca gcgtgaccga cagcgaggag gcgaagggcg cgacgtggta catccacgag
     4741 ctggccagcg gcacctggcg ccgtagcgtg actctgccgt tccaagtcga cgccgatcag
     4801 gcggaggcga ccttcgagca cggcatcctg cgcgtagtgc tgccgaaggc cgagaacgcg
     4861 cgaccgaaga agatcgccat cagcggcagc cagcatgagg ctattggcac gggcgcgagc
     4921 tcgtaaagga tagcggctcc tggcagagcg aagaacgccc ccggcgggac atcccgccgg
     4981 gggcgttttc gtggctggtg ggggaaagag aggggaaact gctccggtcg atggcgttgg
     5041 aggggtgggg gagccaccga ccgggacagt tgtgggggaa ggagacgtca accgaccgcg
     5101 tggctggtcg cggtacggac atttgcctcg aattctcgta acgcacgtcc cttgccacgg
     5161 gttacgccat gtgcgtacgt ctgtgacaat tcggcaccgt ctcacgggcc gaagacgccg
     5221 tcgttgcgcc gatccggagc gtttgccgag cagccggaac gatggtcgga aatgctcggg
     5281 acaaagggtc ctggcataca ccgccgcact cggagtggcg ttctgttcct ctacgcgctg
     5341 gggacgttcg ccgggttcgc gtccggaaca ccgcttgcac acctggagcc gatcaccgcc
     5401 gctgggtggc tgatggagct gtttggcggg taggccggga gtgactcccc ggtctcgccg
     5461 tcgtgaacgg tgatctccag caggtagtcg ccggtggctt ctgcggccaa gggcgggatc
     5521 tccagcatca catccgtgac tagtccgcgc cgtggtctgg gctcgaaggt cgcttcccat
     5581 gtttggtcga ctgccggggt attgagcgtg cgcggcacca gatcgaccgt caccacgacc
     5641 cttccgggca ggtcaaccgc cgtcagggtc tggaaccaaa gcgcgatgat ctccccatca
     5701 atcaccacat tctccagctg cagccacggc tcgaactgcc actcgcaact cgcgggtccc
     5761 gtcagccatg gcggtgatgg gatctccgac aggtcccaga catcggcata gcgcaccccc
     5821 tcgatccaga cggtgtgggc tggctcgaag cgctgcaggt atgcaatggt cggcccgtac
     5881 tgattcggcg gccactcgcg aacgcgccgc tgctggatgc tcacgacata gtagtcggtc
     5941 acctcccaat cttcggcggt gcgaaagccg cccgggtcga ccatgaacgg tggggggagc
     6001 agatagtgga gcggtgccgg ggcgccggag atgtgcacca ccggcgggtc gtcgatttcc
     6061 tggcctggcc ggacgccgga gtcctcgatg atccatcgcg ccgccgcgtc ggtcccctcg
     6121 ccgtagccca tgcgcaccac atcctcggcc gcctcggcac caccgagcag ggggttgtag
     6181 gccacgaggt agtagggcct atgcccgagc gtgacgaggg tttgcgcgag caacagggcg
     6241 atcaacacga tcggcatcgc cacccgcgcg atcgatcgcc gcctcggcag cgcccagcgc
     6301 agggttgcga cgatgccgat cgcggcaatc acatcgagca ccgcatggac cggcagcacg
     6361 tagcggtcga acttcttcgc cccgagtgcc atcccgacga ggtagagcac ggcgaagagt
     6421 agcaggattc cgattgcccg tagcaggcgt gccggcagcg ctctgcggat gcttgggacc
     6481 gccagggaga ccgcggcgag gacgagcccg atcgaactgg ctaccgagag ccgccagagc
     6541 atgctgactg gatagaacag gacaccgggg tcgccggtgt ggagatctcc catgaaatag
     6601 atgggtcctt cgtgaccctc ggcgacggca ccagccacgt acctccgcat gaatgccacg
     6661 gcacgttcgg gcccggtcca cagcgccggc cagagggcga tggtggtgat gagtgccgcc
     6721 gtgagccaca gtgtcccgta gcgggtcgcg gtacgcaggc gtgcccccaa tgtccctcgc
     6781 ctccccatca gcacggtgat gagcagcaca agcccggtca caccgatgag cgccagcccg
     6841 gtgatgcgcg tgagccaggc gatggccgcc agcgccccgg cgacgatcca gggagcagcg
     6901 tcccaccggt cacgcgtcac cgcgtctgcc agtgcgaggg ccgccgccag gctgctgatc
     6961 gtgaccaggc cgtcgatctg gagcagttga tcgtgggcga tcaggaaggg gtcgagcgcg
     7021 atgatggcgc cggccacgac ggtaatccct acgccgaaga ggcggcgaag caagaccagg
     7081 gagagcagga agagcatcgc ctgcaggccg atcttggcga gccgtgcggc gatgaggacg
     7141 tccagtgcgg cgagtcccat ctcccgcagc cgctggtgga tgttgaagac gttgtcgagg
     7201 ttggtatcga aggcgtcgac atagccgggc gcggcgaccc agtagccgac catgccggcc
     7261 cacatcgtcg tcacgcccgg atgggcatgc tggaaggtat aggcgaggtc gccctgccag
     7321 agcgcgcggt agaaattggc cgagcgggcg agccagaaag gctcgtcgac cgtgacgagc
     7381 tggtcgagat cggtgacgcg gaggacgagg tagaggaggg tgagcagggc gagggtgaca
     7441 ggccatcgcc atcgattgga ggtgggtgaa tgcaacaatc gcacgtctcc aacagttgtc
     7501 ctgaggctgc accgctcaca tggcgcatct tgaggatacg cgatgtggta ggcggtggca
     7561 gggatagccg gggcacagcg gaacaaccgg gcagccactt ccgtggtccg tgcaacgccg
     7621 cgaagcctcg cgatcaactg ggtggcacgt tggctgaccc cggcagaaag ggtgacggcg
     7681 gtgccttgtt cagcttgtct gagtaaccgg cgcgccgtag atcgtcacgg tcttgaccgg
     7741 gtcgaacccc ttgagggagc tggcgacttc accgcagggc cggcccggcc agctctggca
     7801 ggcaatgatc cggcggtcgg cctattgaag ccgccatcac cgtgctatac tcgcggcgtc
     7861 atcacaagtg aagtccgaac ggacgttggc aggtcgtgtt gtcgtggtgc gggtccgtcg
     7921 gggcccggtc agcaaaggag gaccagatgc ggtcgttgat ggttcgtctc gcgatgctct
     7981 tcggcgtgtt ggcgatgatc tctgcccctg cggcactggc gcaggagcgg atgtcgatcg
     8041 caaccggcgg caccggcggt gtctactacc cctacggcgg cggcctcgcc gacctcctca
     8101 gcgagcagct cgaagggtac gagttcaccg ccgaggtcac ctcggcatcg gtcgacaaca
     8161 tgtacctgat cgatcagggt gacgccgacc tcgccttcgt gctctccgac acggcctacg
     8221 acgccgtgca gggtaacgag ccgttcgagg acccgatcaa cgcgcgctcg ctggtcaccc
     8281 tctacaacaa cttcactcag gtcgtggtca accccgaatc ggggatcgag tcgctcgagg
     8341 atctcgccgg caagacagtc tccaccggct cgccgggcag cggtaccgag gtgatcgcgc
     8401 agcgtctcct cgaggttgcc gggctcagta ccgacgactt ccagcaggag cagcttggtg
     8461 cctccgagtc ggccgctgcc ttcaaggacg gcaagattga cgccttcttc tggtcgggcg
     8521 gcctgccgac ctcggcgatc accgacctcg gcgccacgcc gaacatcagc tacgaactgc
     8581 tgagcaccga cgagtacgtc gaggagatgc aggagcagtt cggccagttt tacgatgttg
     8641 atacgattcc ggccgacacc tacccagacc agagcgaggc ggtcgagacc atcgtcgtgc
     8701 cgaacgtgct ggtggtccat gccgacatgg acgagcagct ggcctacgac atcgtgcgga
     8761 ccatgttcga gcacaaggac gagttggtcg cggtccactc cgccgcgaac aacctgcaac
     8821 tggagacggc gatgagctcg acggcgctcg agtaccaccc gggcgcagtt cgctactttg
     8881 aggagcaggg tgtggacatc ccggaggcca cgccggccag ctagatcagc agaggaagga
     8941 tcatgagatc gccgtgacca tgtcgggatg ctgatccgac gcatgctgtg gctgccggcg
     9001 gcgacgctgg tggccatggc gctgctcggg attgcgctgc agccggccac gcggctcacc
     9061 gtcacaacgg acgacggccg cgtgctggtc tgcgagcaga tggctccggg cgacacggtg
     9121 acactggtgt tcacgcattc gatgtatggc gtcgaggtgc gggaatcctg gcgcgtggtg
     9181 aacggccgcc tcgtccggac cggcatcgag accgaccgtg ccgccgctgc cgagtactac
     9241 gccacggacg gtgccgtcga gcgcacggac ggcggcttcg cggtgatcag cccgccgctg
     9301 accgtcgatg cccttgtcgt acgagtcgac cagatcggca ggcaccggct gcggtccgac
     9361 ggcactgacg agatctcgct ggcggaccgg gtggagggct ctgctgccgt gaccatccag
     9421 acccgacacc aagccctgct gcagcagggc atcgcggggt gtcggggctg agacgacggc
     9481 gcgcttctct gggggacggg gacggcatga cggacaggga caaggatcgg gaagcgcagc
     9541 acgtcgatcc tcagaccgag gaggcgcagg ccgagagcaa catcgagctc aaagaggtca
     9601 atgtcgctga tttcgaagcc gagagcccaa cccggcagct cagtgggcca atgacggtca
     9661 tcgtcgcggt gatcgcgatt ggcctctctg tctacgcgct ctacgccacc cgcacgacga
     9721 tccccgcaca gacctaccgc accatcttcc tcgcgatcgt gctggtgctc accttcctgc
     9781 tctatccggc ccgtcgccgg aacgccccac tcgtgccgat cctcgacgtg gtcacgatca
     9841 tggtggcggt cgccgtcctg ggctacccgc tggttgacaa cagcatggtg ctcatggcgc
     9901 tgctggcggt tgccgtgccg ctggtcttca tcgtggtgcc cgggctcgca ggcgggaacg
     9961 ggttcagcac gcggtcgctc ttcgccgtga ttccagatgg cgcgctggtc gtgctctcgg
    10021 tgatcgccgc actctatccg ctgtgggact tcgacaactt catctaccgc gcggcgcgtc
    10081 ccaatgagac ggatgtcctg atgggcgtgc tgctgatcct gctcgtggtc gaggcgaccc
    10141 gacgcactgt cggctggatc ctgccggcgg tggccgtcgg cctgattgcc tacggccgct
    10201 tcggcgatat gctcagcggt gcctggggac accgcggcta cgacgtcgac cgcatggtcg
    10261 gcacactcta catctcgctt gagggcatct acggtgtccc gctcgacgtc gccgcgacgt
    10321 acatcatcct cttcacgatc tacggcgcgg tgctcgagtt ctctggtgcg ggcaagttct
    10381 tcctcgattt ctcgttcgcg gccaccgggc ggcgggcctc cggagctggc cgcacgacca
    10441 cgattgccgg cttcctgctc ggcaccgtct ccggcagcgg cgtcgccacc acggtaacgc
    10501 tcggctcggt cgcgtggccg atgttgcgca aggcgggcta cgatccggag tcgggcggcg
    10561 cggtgctgtc agcagctggc atcggcgcga tcctctcgcc accgacgctc ggtgcggcgg
    10621 ccttcctgat cgccgagttc ctccagatct cctatctgga ggtgatcaag atggcgatgg
    10681 tgccgaccat cctctattac ctctgtatct tcctcatgat cgagctggat gcccgccgca
    10741 tgggcaccag ggcggtgatc atcgagacac tgccgctcaa gaccctcacg atgcggtact
    10801 ggtaccactt cacgtcgctg atcgcgatcg tcgtgctgat gatcatgggc tttacgccga
    10861 tcacggccgt cttctgggcg atcgtgctgg ccttcctgct cagcttcctg cggcgcgaga
    10921 cggcgttgac gccgatccgc acggcacagg cgctgcgggg cggggcggtc ggcaccctct
    10981 cggtggcggc gacgacggcg acggcgggca tcattgtcgg catcatggtg ctgaccggac
    11041 tcggcctgaa gatgtccgac ctgatcgtcg acctcgccaa caacaacctc ttcctgacgg
    11101 tgctcttcac cgggatcgcc gtctgggtgc tcgggctggc ggtgccggtg acggcgtcgt
    11161 acatcatcgc ggcggcgatc accgcaccgg cgctgaccac gctcggcgta ccggatgtgg
    11221 ccgcgcacat gttcatcttc tactacgcgg tcctctcgga ggtctccccg ccgacggcgc
    11281 tttcgccgtt cgcggcggcg gcgatcaccg gggcaaaccc gttcaagacg atgatggtga
    11341 cgtggaagta cacgctgccg gcgttcatcg tgccgttcat gttcacgctc aacacggaga
    11401 gcggtgtgca cctgctggcg atcggagacg tggagcggat catcctcgcg acggtgaccg
    11461 cctgcctggc catcgtcgcg ctggtggccg gggtgggcgg ctgggtggtc cagcgggcga
    11521 acatggtcga gcgggcgatt ctgatcgtcg cggcggggtt gctgctctac accggtccgg
    11581 tgcaggacgc cgttggcctg accctgatgg tgattggcat cggcgtgcac tggctgcggg
    11641 tgcgcgggca gggcagcgac accggcggcg agcatcctgt cgcggtggcg tagcacccta
    11701 gaaccacctg gctagtccct tgtgtacgcg tcatccactg ttaccgctag gatgaatctc
    11761 cttgttcttt ttggggctaa tgtgtaggag tacacgggtg catgatccca gccaaaatcg
    11821 catcttggtt cacagaatcc agcaggctgt tagtcgcggt gtagctaagc ctatcgaaga
    11881 agaactgatg agcctgtaca gagtaccgat cgagtcgaca tcgtatgaca atcggggaaa
    11941 gccggcgttc gggttggctg agacggcgct cgtcatcgca gttctctggc cactagttca
    12001 caagttcttc gagccacttg tgaccaccgc cggcgaggct ttccgcaata gagtcttgga
    12061 aacaatacgg aagacacggc atacacctag tggtccccat atttcggttg ctattcagtt
    12121 tggagacgaa tcggatgaag aatttcccga tacccctatc cgctactact tctatcatca
    12181 aattgacgat gaggagttgg tgaagcgctt gaaggcagct gacaagcacc tcaagtcgtt
    12241 accgtgcgag ttgttctcgg agcggtcagc tcccgttgag tatggattct tctgggaccg
    12301 ggagacacaa caatggcggg gatgcgtttg gtgacgatct ggtgagttgt acggtgaagg
    12361 gtggttgcgc gacgacattt acaagaaaga caaggacgat gatgggtagg ctataccccg
    12421 cagagggtca accgcacaga tacagcagtg ctacgaaggt gtttgttacc tcttgtcgca
    12481 ccatgagaca cccgcctctc tgaaggggcg ggtgtcatca gaaaatggaa gctggagggg
    12541 gaatgacgcg acggtaattg gtatggtcgt ggatcaggct aggtcgacct tcggctggat
    12601 gctcggggcg gtcgtcgggc gagccgcctc gccggcgatg ctggcgccga gccggatgat
    12661 cgcgtcgccg gccgcctggc gcatccggtg gagcagactg gattgccggt ctgtagtccg
    12721 ggcgctcgtc gcggtcgtgc ggcgctcggt cgcccgctgg agatcgatgc ccgccaggcc
    12781 gtacgtgtgc agaccctcag cggcggacgg tcggtggctg gtgctggaga tggtcatgtg
    12841 gtcctccgtg atggtcggtc gaatcgaacc ggtcatgcac ctatcatgca ggatgctcga
    12901 ccgccgtaca tcgtagagac cgcgtatttc acggaggagg ggatgcgtac gtaggacttc
    12961 gtacgttacg tacgcgaggc ggcggggacg ataccgagct cgtgcgcccg ggcgatcgcc
    13021 tcgccgcgcg tggcgacgcc gagccgatcg taaatgcggg tcaggtgagt cgtcaccgtg
    13081 cgctcgctga tgaacagctc ctcggcgatc gccgcgttgg acttgcccgc ggcgagcagc
    13141 cgcaggatct cccgctgccg gtcggtgagt ccggcgtcct tccgctcagc cggtggtggc
    13201 tcggcctggg acggcgcctc ggaacgcatc cgggcaatgt cggttgccag ggcgatcgcc
    13261 tcgggaatag acatccccct gccagcgttc atctcccggc gcaaggtgac ctcatcgagc
    13321 accgcgcgga gacgcgcatc ggtccgagcg agcatctcct cggtttggct gtgctgcatc
    13381 atgccgaggc gctccagaat ggcggacgcc gcgccgcgga gacgcgcgcc atcaacaggg
    13441 cgatcgcaga gcgtcgccag ccgcgcggcg ctgcagagcc gatcggcgag tgccacgggc
    13501 tgccggtggg gcgggtcacg gtggtaggcg agactcgcct ccagagtctc gcccgcctca
    13561 tcgatgtcgt tgcgggccag caggagatcc accagtgcac ccagcgccag cgccttgacc
    13621 tccggccggt cgtgctcaat ggcctccaac ccctcgcgca gcagcgcctc tccgcgctcg
    13681 gcttgaccat gctcgatcag ctgctccccg atcgtggcga ggaacatgcc gccccgcgtg
    13741 cggtcgggtg ccttgtggat cgtttccacc gccagcagct gcatccgctc ggcttcttcg
    13801 gcttcaccgc gttgccagtg gatcatgccg atgaggtttt gcgcgttcgc cgcaagccgg
    13861 gcgtggccga gctcgtcggc aagcgtcagg gcgcgacgag cgctggccaa tgcatcgtcg
    13921 ggcacctcat tggcaaaggc ggcgatcgcc gccatgtacc agaagcgcaa ctgaatctcc
    13981 ggcggcatgg cgtcggcgta gggtgcgagc cgttgggtcc agactcgggt ttcccgaatc
    14041 tggccggtct ccatccagaa cgagacgagg gcggagacca tctgcacggc acgcgtccag
    14101 tcctcttggg ccacggtccg ctccaaggcc acctgcaggt tatccgaatc ccgctcgtaa
    14161 cggaccgtcc agacgcgcaa ttcggacgtg ccggtgcgcc agtgctcttc aggctggtcg
    14221 atgacggctt gggcgaacca tgcggcatgg accccggcga tgcggttcgc ctcgccgctg
    14281 acctcgagct gctcctgcgc gaactcatgg atcgtgttga gcatccgcac gcgcgggccg
    14341 gtgtccggtg gctcgaagat gcgcacgagg ctgtggtcgg cgagcacgcc gagcagttcc
    14401 tccgtgcgat gcccgagcgc accgtcggcg ttgcagaccg gctcgaccgc atcgaacggt
    14461 gcgctgcccc ggaagatcgc cagccgccgg aagagcgcgc gctcatcgtc gtcgagcagg
    14521 tcgtggctcc agtgcagtgt ggcgcggagg gtctggtgcc gctccggggc gtccctcggg
    14581 ccaccgacga ggaccggcaa tcgctcggag aggcggtcac ggagcgcggt cggcgagagg
    14641 aaccgggcgc gcgcggcggc cagctcgatc gccagcggca gcccgtcaag ctgctggcag
    14701 atatcgtcga tcgcgcggac ggtctcccgg gtgacctcca gatcaagccg caccgcgcgg
    14761 gcacgctcca cgaagagctc agtcgccggc gcgagctcgc tgatggaggt gctggtgacg
    14821 ggcagaggct cgatcggcac cacccgctcg gcggagaggc gcagcatcat ctggctggtg
    14881 acgaggatct tgaggttggg gcagcgggcg agaagctgac cgagcagggc gaggtgttcg
    14941 gcgacgtgct cggcgttatc gagaatgagc agcaggcgtc gggagtcgac cgcggagatg
    15001 atgcggtcct cgatcggcag ggtatcggac tggctgatgc cgatgccgtg ggcgatagcg
    15061 ggcagcacct cggcggcctc gtcgaccggc gtgaggagca ccagcgcgac ctcctcggcg
    15121 atccgggggt cgaggtcgtg ggcgatctgg agtgcgaggc gcgtcttgcc gacaccgccc
    15181 ggcccgatga gggtgaccag gcgggtgtcc ggtctcgaga gcacctcggt aatgcgggca
    15241 agatcgcctt cccggccgac gagcgaggtt gccggcaggg ggatatgctg gtggtggagc
    15301 gcgatcgcct gcatggctgc ctcatgtggc tgctgatccg tccgggtccc gtgtcgcgtg
    15361 aagtatagaa ccgatgatgt gcggggacac gctgcgcgtc accgaagggg ataccatgcc
    15421 atgcctgaca tcaccgtgaa gcccgacgtc gccttcgccg gtgacaatcc cctgctgatg
    15481 ctctaccggc ccgatacgga ggaggtgatc gccgtcgcga gctattggcg gtgcgcgtgg
    15541 tcgccggagg gtgccggcga ggcgctggtg atctgggtcg acccggcggc atctggattg
    15601 gacgagcagg cgccggccgg tatctttgct gacaacacgg caatggcgca tctcgtgtgg
    15661 gtgcagttca acagccagtt cgaccgtctg cagggtcgcg gcatcgagga tcgggaaatc
    15721 cagccggcgc gattcaccat gctggcggac ggcatccgat tgcaccgggt gacctgtgca
    15781 gccggggcga cgacgatcga gctggagtgg cgcgacgcgc tggaggcggt gcacatggtc
    15841 gccacgccga gggtgggcag cagccgctgg gaggtgacca acgtggtgct gccctgcgcg
    15901 ggcgcggcga tccgggttga cgggatcgag atcgccgggg aggtgcacca gccggaggga
    15961 atctaccgca gctccgcgtt cctcgccttc gcggagtcct gggtgcggct ggccgatgcc
    16021 ggtggcgaat cgtagttgcg gactcggaac ctgtccggcg ctgagcgagg gcggcatggc
    16081 gatcgatgta atgccgttcg acgctaggat cgacgtctct gcactgtcgt cctcgaggtg
    16141 ccaggtttcc atcgcgcagt cgcccctcgc aggccggttc cggcggccgg caacgagcat
    16201 tggtcagcct tgctcggcgc cgagatgggc gtggcatgcg ccatgcgtcg tgctcctagc
    16261 accggacagt gtcgctggat acgacggata ccgacaggag aagggtcgga tggatggcag
    16321 cgggaacgag caacagcgtg agcagaagca gccggagaac gccgcctaca ttacgctgat
    16381 cggcatcttc ctgggtctct tcgccgcatt ctcgaaacga gaacaggaga atgagtcgca
    16441 gctcaggctg actggcctcg acctgacaat gctgggcttg gcgtcctttc gtaccggccg
    16501 gctcactgcc tacgaccagg tcaccaagcc gctgcgcgaa ccggtcacga aaaccgagtc
    16561 ggacagctac ggcaccagcc agacgaccgc tccggagggc agcggcgtgc gcaaggcgct
    16621 cggtgaactc gtcgcgtgtc caacctgcac cgggacctgg gtggcggcct tcctcgtcta
    16681 cggcctgcgg gtcgctccgg ggccgacccg gctgttcctc gccttcatga gtgcgaccgg
    16741 tcttgccgaa ctgctcgaca gcgccaacga ggcgctgttc tggacgggcc gtgccgagcg
    16801 caagcaggcg aagccgccca ccagttaggt acacacaggc tggatcccaa gtggatcggg
    16861 tgacggaggg atacacgtgg ttcggctgct gctcacccac ggacgtggcc tgagtggccg
    16921 caatcaggag tatcttcgcc ggaagttcac ccgcctcgtg caggagggcc tccgaaacgt
    16981 cgggtacgac gacctgacca gcgagcaggt cgactttctc tactacggcg atctcctcga
    17041 tcgtgccgag cgggagtacc tgcccgagcc cacgccgctg atcgagatcg ggcggcgcga
    17101 aggggcggag gtttcccggt acgacttcaa ggacgacctc gagcacctcc atcggcatct
    17161 cggcgcactc gcggtggccg atgccggaat ctgcaacatc gtcgaggagc gggcagggca
    17221 ggtgccgatc gtctctgagg cgagcgacga ggcgtcccgc cgcgccgcga tcgagatcgc
    17281 actggaggac cgtaccgcgc aactgctgga gaacccgaac gagctgctcg acgtgctgga
    17341 gcgcgggttc ggccctgcct acggtgacga cgaggcggtt cgccggcacc ttcacgagct
    17401 ggacgagcgc ctcggtgatg acccacgcct gatcgctgca cgcagaggcc atgaggaaga
    17461 ggggaccgat gacaccaatc gcgtcgagga tttgctgcgg gagtggattc ttggtccggt
    17521 gaccgatgcc attgccagtc tgcaggcggt cagccggatc attggtgact acggcatcga
    17581 tctccggaag cggctcggcg agtggcgctt ggccgcatgg agcgaggcgc agactcgctt
    17641 cgaggggacc gacttcggcc gccaatgggc cctgctcttc gacatcatcg ctgcggtcgc
    17701 ccacctcacc atgctggacg gcgtctacat cgcgcgacgc atggccgacg tcgagatcta
    17761 cttccgcgac ccctcgatcc gcaacgacgt ccggatcgcc tttcgcaacg cggtgctggg
    17821 agctgacggg gcgacgattc tggtcgcgca cagtttgggc tcagtgatcg ggtacgacgt
    17881 gctgcgggag tacgagcgtc tgcaggtgcc gggcctagtg acggtcgggt cgccgctctc
    17941 gatcggccat tttcggcgca atcttgcacg taaaggagag tcaggtgaca acctgccggt
    18001 gccgtcgatg ctggagggat gggtgaacgt ctattccgag atggacccgc tcgttctcgg
    18061 tagcggcatc gagcggtggt ttcagggcgg tggtgacgag tccggctcgg caccggttga
    18121 ccgtgaggcg ggcaacaccg gctatctgga tgcccacagc ccggaccagt acctgcggtc
    18181 gaaggaaacc gctagcgcca ttgtccggat ggtcacgcgt gcgatgcgcg acgtgcgatc
    18241 gtccaattgg tagccccgta gccccggatt tggatttaat tgcgtgggtg acgggtggga
    18301 cccagtatgg tgtcgtcagt gccgcgcggc cggttctgcg cgaatcggct ggctggtgcc
    18361 gggagggaag ggggtgcgca ttgaccgcac cgcgcgggat gacggataag gaggggaggg
    18421 gtttgaatgg ttgcctcggt cgcgaccgac caccgtcgga cgtaggtctg accgaactgg
    18481 gacccggcga gcgatcactc gttgatcgtc ctgccgcagc ggccctcgat ccacgttccc
    18541 gatcgagtca gcatcgggcc cgtgatcgtg cgcgtgctga ctcccggcgg aacccgggtc
    18601 gagggccggt gagcgcccca ttgcgctcct gccgcagacc gacgagagac aggagggtct
    18661 cgatgggccg caccgagatc tccctcgctt caagcgacca caaaccgatg ctgccgtcca
    18721 attcagcatc gcctcggagc cctgcccggc agcctggtcg cgtgatgccg gggccattgg
    18781 ggcgaggagc cgctggtgac ccgggccggc gactgccaat cgccaccctg gcgaactggt
    18841 atcctgccga cctgatacca gtatgcaggg gcaggtatcg aggcacatcc gtattcttac
    18901 gtatttgtca cacaatcgtg ctcggggtcg gtatcgtcct cgtcgaagcg aggttactcg
    18961 cccccgatct gccaccaacc gagcgccggt gtctccagcg cagtccaggt ggcacccgcc
    19021 gcgatcggcg cgagccagct ggcgaacggc atattgctgc ccgccgtggt ggtgctcagc
    19081 agcaggcacc gcgcgtccgg cgagatcgac agccgcggca ccaagcccgg ctccatcccc
    19141 tccggcatcg gcgttaccca ggcctcgccg gtggcgccgt cctgcaccag cacggtgccg
    19201 tccggccgcc cggtgaccag cacgccgtcg cgaaagtcgc ccgccagcat ggcctcgctc
    19261 aggacagtct ggttgtcacc ggctgtgggc agatctgtgg cgatctgtcc ggtgcgcgtg
    19321 tcgatcagca cggtgctggc cgggtcgcct gcaccggtcg ctccagcgta ggtccacccg
    19381 gcgagcgggt agatccatgg gtaggcgttc aactcgggaa gcggggtggc ggtgtcggtg
    19441 gcggcgtcat agagcataac ctctggctgc tcgggggtgc cggcgtcgta gatgatggtg
    19501 ccgtcgaccg ggttggcaac gcgaatgccc tcgccggtga tccccggcgc gttggcgggc
    19561 tgcgcctccc cggtggcgag atcgattcgg gtcagcgcac cgtcgactag cgccagcagc
    19621 gcggtgccgt ccggcgtgaa gagcagtggc acgcgggcgt ccggggcgcc gggcgcgagg
    19681 taggcggtcg gctcctcgag ggcatcgagg ctcgccacca ggacctcatg ggtctcgccg
    19741 aagggatcgc cgctgccggt cgcccacgcg acctgcgagt ggtcggggct gaagacgacg
    19801 ctgcggttgc cggcgaagag catcggatcg gcgtcgtgga agagcagtgc ctcgggttcc
    19861 aggtcgacaa aggccgcccc ggcgaggtcg cctgggatca cgagggttgc tccgacattc
    19921 gcgaagggcg tcggcgtggc cccgaggccg gcgatcggct cggccatctg ccggaagcgg
    19981 atcgcggcga tcccgcggtc gtcgccgctg ttgggaagcg cgtaggcgtc cgcggcgacc
    20041 gggaacggcc cgccgagaat ctcgctcgtg gtcgccgatt cgccggtgcg catgtcgacg
    20101 atctgccagt cgaggccgtc atcggtgatc ggggcggcga cgtaggggcc ggtctggacg
    20161 aagggtgcag tggcgggggt catgccggcc tgaaagtccc ggtactccac cgggtcgaag
    20221 agccacgtct cgccggttgc ggtgctcagc accatgtggg tgccgtcgtc ggcgagcacg
    20281 atcgccatgc cgggttcgag gcggagctcg ccggcgggga gatcgtgagt ggtgccgtcc
    20341 ggcatggtca gggtgatgcc agtctcggtc tcggcgatcg tcaccgcctt gccgccggct
    20401 gcgccggcga ccacggacac cgtgcccggc gcggcgcaac cctggaggct cgccccatcg
    20461 gtacctggtg tctgggcagc ggtgagggcc ggcagcatcg cggcgatcgc gatcagcagg
    20521 gtgagggtta cggcggctgc gcgtagctgc gggagaggac acatcgctca ggtcctttcg
    20581 ggtcgtgatg gcgcgtcggt ggacgcggga tggccaagtg gagccattgt gccacaggga
    20641 gatgctcgca tcgtggtcgg cacgattcct gcatcgagcc cggcatgacg gacgagcgac
    20701 cggactcatc accaccacac cagccacccg atcccgtacg ggagccgcga ccgcccgacc
    20761 aggcagagga gcgggaggaa aagcaccgcg agcgctggga gctgctcgac aacctgcagg
    20821 cgctgctcga gccggcgatg accgtgctcg ggctcgtctt cctcgtgctg ctgctcgtcg
    20881 actacgcgaa cacggagctg ggaccgctcg ggcgcgtgcg gatcgaccag gcgttgcagg
    20941 tgatctgggg cgtcttcgtg atcgacttcg cggtgcgcct ctgggtggcg ccggcgaagc
    21001 tcgccttcct gcgcgccaac tggctgacga tcatctcgct ggcgctgccg ttcctgcggc
    21061 cgctgcgcgc cttccgggcg atccgggcgg tgcgctcgct gagcctcgtg cgcctgctgg
    21121 gcggcatgaa ccggggcatg cgggtgatcc ggcgcatcac gcggggcgcg cagctcgcct
    21181 acgtggggct gctgacactg gtggtgatgc tggccggcgc ggtcggcgcc tacttctttg
    21241 agcagggggt ggccgacgcg caaatccgga cgttgggcga tgcgatctgg tggtcgtcgg
    21301 cgctggtgac cacgatcaac aacgagaagt acgtggtgag ccccgaggcg cggacgatcg
    21361 cgatcctgat gcgcatctac gcggtgagca ttttcgggta catcaccgcc tcgatcgcga
    21421 cctacctcat cggcagcgcg gtctccggcg agggcgaggc gctggagagc gagaacaccg
    21481 ccctgcgcgc cgagatcgca tcactgcggg aggagatccg ggcggtgcgc cgctcgctgg
    21541 agcgggacga taacgcccag gaccatctgc aggagtgaac tctgaccgat ataggcccgc
    21601 cgtctgcagc caagtgcagg atggttgagg caacgctgga ttgagcccgg agtgcggacc
    21661 gacataagag cctgtgttga gtgccgccga agagtcctct cggccaccgg cccgagtcaa
    21721 atgggtcgaa gcatctcttg cgatccatgc tccgacactc gatgtctggc aatcccggcg
    21781 caagagatgc ttcgactccg gtccctcgga cctcggctca cgatgacggc agtcggtggt
    21841 tgcgggtggc agacctgtgt ccgaccagac tcgcccttca ggtgccatgg catgacccgg
    21901 agccgctgtc agtgcacgta gctcgcgccg ttgacgtcga ccgtcgcgcc ggttgcatgg
    21961 cgggccagcc ccgaggcgag gaagacgacc acgttggcga cgtcctgcgg ctgggcgacc
    22021 tcgccgagcc cggtgtccgc tgcggcgacc tccaccccga ccgtctcggc aaagacctgg
    22081 ttgaagggcg tgtcgacata gcccggcgcg accgcgaagg cggtcacccc ctcgcggccg
    22141 tactgccggg cgatggttcg cgtcatcgcg acgacacccg ccttggccgc ggcgtagtgc
    22201 cagtaatccg ggtcctcgcc tcgaaaggcg gcgcggctgg cgaggttgac gatgatcccg
    22261 ccctcgcgct gtgctcggaa cgagcggact gccgcccggc agagcaccgc cggagcgatg
    22321 aggttgacgc ccagtgagcg atcccagacg gcgacccagt cctccagtgg gtcgtcgatc
    22381 ggcgcgggcg ggtagatccc ggcgttgttc accagcacat cgatatgccc atatgccgcc
    22441 agcgcacggg cccagagatc gtcggtggct tccgcatcga cgaggttggc gacgagtgca
    22501 gtggcgcggt cgcccagcac ttcggcgagg tccttcagcg atgccttgtc gcggtcgtgc
    22561 agcaccaccc ggccaccggc ctggacaatg gtgtgggcgg tggccgaacc aatggcgccg
    22621 ccggcgccgg tgacgaggac gatgcggctg tcgagatcga tcatggcgag aactcccagg
    22681 tgctgaagcg agtggcgttg cggaacagtg tgtcagacgg cgcggcggcc ggtgatcatg
    22741 ttgtagatca ggatgacggc ggcgatgatc agcagcacgt ggatgagcga gccgccgatg
    22801 tcgagcagca ggccgagcag ccagaccacc accgcgatga cgagaatcgt ccagagaatc
    22861 cccaacatga ttatctctcc ccggcgccgc tcggcaacgc tccccctcga tcgccctgcg
    22921 ccggtatgtg accagccggg acctgccgtt gacggttctc cgctcgctgt ctgtacgatt
    22981 gtacggatga gcgacggtgt tggcgaaggg ttaccagccg agctcgccat tctcgtaccg
    23041 gaccaggacc tttgcggtcg tcgatccggc ggccagaggc gcgaaggtat cgggcagctc
    23101 ggcggcggat atgcgcaggt cgaagagccg ctccagctca tgcccggttc cgtgcactat
    23161 ggggaagtac cagcgcgcgt gcgcggcgta gtccggacgg tcgctggagc cgatcacgat
    23221 cagctcgcgc tcgtggaaca tcggcgcgag catcagtggc tcgatgttcc cgtcggccag
    23281 gacgcagatg cgcccgcctc gctagagacg tccctgcagc agcgcgaacg ccgcatcgcg
    23341 gctggagcac tcgacgccgc cgagatagcc atcgctcagc cccccggcgt cctccgcgcg
    23401 tccggcgcgt agccaagctc ggtcaccacc tcgcgcatcg tctgataggc gcggaactgg
    23461 accgacttga ccgccgggag ggtcatgttc tgggcggtgg cgatttccgg gccggtgagc
    23521 cccgccagcc gcagctcgac gacgcggcgc tgccgctcgg gcaggtgccc gagggcgatc
    23581 cacactgcgc gaccctcgtc tcggctgatc gcggcgtcgt ccggggcgcg atcgggatcg
    23641 ggcgggtcga aggcgggacg gttccgcagc ggatggagca ggcggtcgag tgagaggtgg
    23701 aagcggcggc gtcggtgatg gtcgacgacg gtgttgtggg cgatggcgaa gagccaggcg
    23761 cggaaggtcg cgcccggccg ggtcacatcg acgcgataat cgggcaggga gcgcagcacg
    23821 cgctcgaagg tgcgcgaggt ggcgtcctcc ggcctccggt gattcgagac ggcgcaggca
    23881 gtagcggtag accggctcgg accagctcgc gtagatgtcc gcggtggcct cggggtcgtg
    23941 ctgggcggcg cggatctggg ccggggtggt ctcagggccg gtccgctccg tacggtcggt
    24001 gcgctcgtcg ttggatgtcg ctatcgtgcc ggcatccatg ccgcgtgcct ctcgtggctc
    24061 cggccggtta gcccggaggt agtcgatcgc ctatagagag ggtaaacgcg tcgggtggga
    24121 aagggttgcg gtgcgagtcg caaagccgcg taccatcggc gtgggcagtg gtgcagatgc
    24181 cgtggaacgg ctttcgccta tgcgacgtcg gtgtaggtca caaccgcctc aacgggatag
    24241 tggtcgctga cgggcgcatc ctcggatcga tggcggagga tctcggcagc gaggacgcgc
    24301 aggtttcgtg tcaagggcca atcgatgtac cacgtcccat agcggtcggg tacgtagcgc
    24361 tcgccctcat agttgtgaaa tgaggcgggc gggggccaaa taccggcgac cgagcggtag
    24421 gcgtccatga acccggcttc agcgaagagg ccgaaggtgc gtggcgtgta cgcggcgtgc
    24481 gaggtcgggc ggtaggggct ggagttgaaa tcgcccagga tgatgatcgg cgtctggggt
    24541 gaccagccct ccagtacctc cagaatcacc tgcgccccgc gcacgcgtgc cggctcactg
    24601 atgtggtcga ggtgagtgtt ga
//
LOCUS       DASYWL010000014         5246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_657457, whole genome shotgun sequence.
ACCESSION   DASYWL010000014 DASYWL010000000
VERSION     DASYWL010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657457"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            514..1083
                     /locus_tag="VGR22_00545"
     CDS             514..1083
                     /locus_tag="VGR22_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV2127089.1"
                     /translation="MNIGYARVSTGEQTLDLQLDALEKDGCDKVFTETASGAKSDRPL
                     LQDALAYARPGDTLVVWRLDRLGRSLPHLIDTVTDLRERGIGFRSLTEHIDTTTPGGK
                     LIFHVFGALAEFERDLIQERTHAGLAAARARGRVGGRPRKLTPGQVALARRLYADGET
                     DIATICQTLGVSRATLYRVLKNSGDAGKS"
     gene            complement(1181..1603)
                     /locus_tag="VGR22_00550"
     CDS             complement(1181..1603)
                     /locus_tag="VGR22_00550"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEV2127090.1"
                     /translation="MRVMVLVKSSPETEAGQMPTEQELAEMGKYNEALANAGIMLDGE
                     GLHSSSKGVRIDFSSEGTTSVTDGPFAETKELLAGFWLWKVESMDEAVEWAKKAPFGD
                     GAQLELRQVFDDEDFGEAFTPELREQEQRIREQSQTQQ"
     gene            complement(1879..2073)
                     /locus_tag="VGR22_00555"
     CDS             complement(1879..2073)
                     /locus_tag="VGR22_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127091.1"
                     /translation="MGAILAMLGYVVPTLHGPPIFVAWQANALMMATCSAVVMVVELA
                     RAQSNPITHAPWPGSAATPV"
     gene            complement(2183..2488)
                     /locus_tag="VGR22_00560"
     CDS             complement(2183..2488)
                     /locus_tag="VGR22_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127092.1"
                     /translation="MDIYSRIRQAILDKHQIIATYRGHRRKRCPHVLGTKGGRRQALF
                     FQFAGSSSSGLPPGGEWRCIPIDQLADVVVQPGPWHTRTGHIRPQTCIDVVDAEMAY"
     gene            complement(2656..3096)
                     /locus_tag="VGR22_00565"
     CDS             complement(2656..3096)
                     /locus_tag="VGR22_00565"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEV2127093.1"
                     /translation="MIFLDANIFLRALTRSSDPDVQRMNQIAGELLRQAERGEVEVTT
                     SEAVIAEVAFILTAKTHYRLPVPDAAGRLAAILRLRGIKLRQKRVVLRALDLWSAHPA
                     LGFVDALTAAYAQQPGVELATFDTDFDHLPNIIRWQPEDGGGAR"
     gene            complement(3093..3365)
                     /locus_tag="VGR22_00570"
     CDS             complement(3093..3365)
                     /locus_tag="VGR22_00570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEV2127094.1"
                     /translation="MKEFISTISSKGQVTIPADVRRQLGVRSADKVAFVVTDAGTVEV
                     RPARFTLESVLGSLDALPDESVDLDREIEEAVEEGVAQKMRHQEGR"
     gene            complement(4309..4431)
                     /locus_tag="VGR22_00575"
     CDS             complement(4309..4431)
                     /locus_tag="VGR22_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127095.1"
                     /translation="MTEQTTPTPADNEWGPWEGKAAEVTLTGPDGSASARDDAV"
     gene            complement(4428..>5246)
                     /locus_tag="VGR22_00580"
     CDS             complement(4428..>5246)
                     /locus_tag="VGR22_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV2127096.1"
                     /translation="DEYGRYVARFLHGLADPAQATPGMVHAFAYAPTQRGTTPRPSTV
                     MVRLAAVHGYYDLARRVGMIERNPAADVRRPRTTDPRPKGLSSDELRALLNATPRTPP
                     GARDRAAILTAVLTGLRRSELLGLTVGDIETKAGRVFYSTRTKGGHMRYRELPAPAWQ
                     AIRRALIALGTPYETLEPEAPLFPISGRGFAANLRRYAKRAGLEGVTPHVLRHSAAKL
                     RRETGASIEEIGTFLGHRSLHTTSVYLRQLEGETDPGWHGVAVALGLDGEEVNA"
BASE COUNT          871 a         1773 c         1655 g          947 t
ORIGIN      
        1 ctatcaggcc attgacatgg tcatcggtga tcttggtggg aaggggtggg agctggtctc
       61 agtcactgag gtctttcgcg ccagcgacga ctactgggag aagaacctgt atttcaaacg
      121 cccgcatgat cccaaccggg aactgaagga ctaaatccac aaccacgtcg acaccgtcgc
      181 agcgtccaag ccggcgatgt cggtatgtga tccctttcgg cagccccttg tgaccgccgc
      241 ggttgggcac gccatcggct ttccgacctg gcagtcgctg gtgcgggagc aggggctcga
      301 cgagaccgct gccgtcgaga cgatggggac ggaggggcgc agcagcgaac agcctgctct
      361 gcttcatgct gtctcacaaa cgtccgtttt tgcgacaccg gcaggaccgt gctccccacc
      421 cccgcttttc ggcccgattg ctgtcgcaca acccgtctca gaatcaagat tttactactt
      481 gcgcaccccg gtaagttttg agacgatgaa tgcatgaaca tcggctatgc ccgggtctcc
      541 accggggagc agaccctcga cctccagctc gacgccctcg aaaaagacgg gtgcgacaag
      601 gtcttcaccg agaccgcgag cggcgcgaag agcgaccgcc cgctcttaca ggacgcgctc
      661 gcctacgccc gccccggcga caccctcgtg gtctggcgcc tcgaccggct cgggcgctcg
      721 ctgccgcacc tgatcgacac ggtcacggac ctgcgcgagc ggggcatcgg ctttcgctcg
      781 ctcaccgagc acatcgacac caccaccccc ggcggcaagc tcatcttcca cgtcttcggg
      841 gccttggccg agttcgagcg cgacctcatc caggagcgta cccacgccgg cctcgccgcg
      901 gcgcgcgccc ggggacgggt cggcggccgg ccacggaagc tgacgcccgg ccaggtggcg
      961 ttggcgcggc ggctctacgc ggatggggag accgacatcg ccacgatctg ccagacgctg
     1021 ggcgtctcgc gggcgaccct ctaccgagta ctcaagaatt ccggagatgc cggcaagagc
     1081 tagcacgaca cgagagacaa cctgcctccc gcgacgcgat ttctcgactc cgggctcgct
     1141 gcgcacggcc tgcgcgcgaa atgactgtag cgaccgcccg ttactgctgc gtctgactct
     1201 gctcgcggat gcgctgctcc tgctcccgca gctccggcgt gaaggcttcc ccgaagtcct
     1261 cgtcgtcgaa cacctggcgc agttcgagct gcgcaccatc accgaatggc gccttcttcg
     1321 cccattcgac ggcttcgtcc atcgactcga ccttccacag ccagaacccg gccagcaact
     1381 ccttcgtctc ggcgaaggga ccgtcggtca ccgacgtcgt gccctcggag gagaagtcga
     1441 tccggacacc cttggagctg gagtgcagtc cttccccgtc gagcatgatg ccggcgttgg
     1501 ccaacgcctc gttgtacttg cccatctcgg cgagctcctg ctcggtcggc atttgccccg
     1561 cttcggtctc gggggaagac ttgacaagaa ccatcacgcg catcgcagcg tttcctttcc
     1621 tcgtgtggcg cctggtacag cgccatctga atgtttgcac tgccacggct tcgccccgtg
     1681 acacctggta gtcgaacgag ccgaccacaa atcgacatgg tcccgagcgg cgcccgcgcg
     1741 tggatctacg tcagcaccgt ctgcgtgctg gtctcgccga gaaccccacg cgtggtggtt
     1801 cgttccgtag cgcaatcaac gtgacacccg atcggcctcg accatcgacg gcccaccggc
     1861 cgggggcaag cacccgggct acaccggcgt ggcagcgctg ccaggccaag gggcatgggt
     1921 gatggggttc gattgtgcgc gggcgagttc gaccaccatg actacggccg agcaggtggc
     1981 catcatcagc gcgttcgcct gccaggccac gaagatgggc gggccgtgga gcgtcgggac
     2041 gacgtagccg agcatcgcca ggatggcccc cacgaacttg gactgatcgc tgtcacgcag
     2101 cgcctcgagc agcgcgatac ctattgccct ctgccggcga gctgaggtgc gcgtaccgag
     2161 tcgtgaggct ctacccctcc tatcaatagg ccatctcagc atccacaacg tcaatgcagg
     2221 tctggggacg gatgtgtcct gttctcgtat gccacgggcc tggttgcacc acgacatcgg
     2281 caagctgatc gatggggatg cagcgccact ctccgccagg cggaagtcca ctgctgctgc
     2341 taccagcgaa ctggaaaaag agtgcctgcc gtcgtccacc cttcgtgccg agcacatgcg
     2401 gacacctctt gcgtcggtgt ccacgatagg tggcgatgat ctggtgttta tcgagaatgg
     2461 cttggcgaat ccgcgaatag atatccacta ccctaccctc ttctctgacg tctatggcgt
     2521 cgctggccaa gcacgcctac cgcgatcgag aaagctttcc attacctcca gacggttctt
     2581 ccgccttgta agtactgagt ggaagttctg agaagatcct gggatcggtg aaggggtggg
     2641 gatactgctc agcaatcatc gcgctccacc tccgtcctcc ggctgccagc gaatgatgtt
     2701 gggaaggtga tcgaaatcgg tatcaaaggt ggcgagctcg acacctggtt gctgcgcata
     2761 ggcggccgtc agggcatcaa caaatcccag ggctgggtgg gctgaccaca gatcgagtgc
     2821 ccgcagcacg acccgcttct ggcgcagctt gatgccccgg agccgcagga tggccgcgag
     2881 gcggccggcc gcatcgggca ccgggagccg atagtgggtc ttggcggtca gaatgaaggc
     2941 cacctccgcg atcactgcct ccgaggtggt gacctcaacc tcaccccgct ccgcctgccg
     3001 gagcagctcc ccggcaatct ggttcatccg ctggacatca ggatctgatg atcgtgtgag
     3061 cgcgcgcagg aaaatgttgg catccagaaa gatcatcggc cctcctgatg ccgcatcttc
     3121 tgcgcaaccc cctcctcgac ggcctcctcg atctcgcggt ccagatcgac tgactcatcg
     3181 ggcaacgcat caagcgagcc cagcactgac tccaaggtaa accgcgccgg gcgcacttct
     3241 accgtccccg catcggtcac cacgaaggcg actttgtcgg ccgatcgcac gccaagctgc
     3301 cggcgcacat cggcggggat cgtcacctgg cccttgctgc tgatggtcga aatgaactct
     3361 ttcatggaat ccctcacagc caggacatgg ctcgcgcgta aggatatagt acacctatct
     3421 ttactacgag tcagcatctt tacatcgaga gagatgtagc cgctcgtgag cgggatcggc
     3481 cggggtagac ggggatctcg tcggtgttgg cacgtctcaa ccgatacccc atccttacga
     3541 cgaccgaggg actggccgtc ggccgcgacg atctccgtca tcatgcacac cttgcccgcc
     3601 tcggcctgaa gcgcgtggtc ctgcagccgc ccctccacgt agaggggatc accacgctgt
     3661 ctaggcctcc tccggcatgt tttcgtgcgc tgcaagccat tgccggagct cggctgctcg
     3721 ggagccccga gctcggcgtg gtagcgggca gcaacggcaa gcgccaccgc gctcgggata
     3781 ctggctggca tccatggggt ggtgcacggt gcatggggtg tccaaaacag atagcacgac
     3841 gggtgcgatc aggtggttgg ctcggcgtca gtcccgtccc cgtcgttaag ggtgatgtcg
     3901 aattcccata cagtgtcgtc gagcacgagg tggtccagct caaccccgtc caagccccca
     3961 gcatctgccg gaacgctgcc tccttggccg cctcgcgctc ggctcgcgcc tggcgccgtg
     4021 cctcgatcat ggcctgggcg gcggcgcgca tgcgctcgcg ccgggcggca gcctccgcct
     4081 caacgcgtcg ccagttggcg ggccagtact tggagtacca gccgtccttg taggttggcg
     4141 agcccatacc ccagcgggac ttgcccccgt gcatccgata gcgcccgctg ggccacagcc
     4201 cccagttctt gcatggggtg ccggcgcggg tcttggcccc gcaccgccgg tcctcgggca
     4261 cgtcctgcaa gttgctcacg ctagtcctcc tatcagttct ccggagggtc agacggcatc
     4321 gtcgcgggcg ctagcgctac catccgggcc cgttagcgtg acctctgcgg ccttgccttc
     4381 ccacggcccc cactcattgt cggctggcgt cggtgttgtc tgttcggtca tgcgttcacc
     4441 tcctcaccat cgaggcccag ggcgaccgct accccatgcc agccggggtc ggtctcgcct
     4501 tccagctgcc ggaggtacac gctggtcgtg tgcaagcttc ggtgccccag gaacgtgccg
     4561 atctcctcga tcgaggcgcc ggtctcgcgg cgcagtttcg cggcggaatg tctgagcaca
     4621 tgcggcgtca cgccctcgag gccggcccgt ttggcgtagc gccgcaggtt ggcagcgaag
     4681 ccgcggccgg agatcgggaa gagcggcgcc tccggctcga gggtctcgta gggcgtgccc
     4741 agggcaatga gtgcccggcg gatcgcctgc cacgccggcg ccggcaattc ccggtaccgc
     4801 atgtgcccgc ctttcgtgcg tgtcgagtag aacacgcgcc cggctttcgt ctcgatatcg
     4861 ccgacggtga ggcccagtag ctcggatcgc cgcaggcccg taagcactgc ggtcaggatc
     4921 gccgctcggt cgcgagcgcc ggggggggtg cgcggggtgg cgttgagcaa cgcccggagc
     4981 tcgtctgagc tgaggccctt ggggcgcggg tccgtggtgc gcggccggcg cacgtcggcg
     5041 gccgggttgc gctcgatcat gcccacgcgc cgggccaggt cgtaatagcc gtgcaccgcg
     5101 gccagccgca ccatgacggt tgagggccgc ggcgtggtgc cccgctgggt tggtgcgtag
     5161 gcgaaggcgt gcaccatacc cggtgtggcc tgtgccggat ccgcgagccc gtgcaggaag
     5221 cgggcgacat atcgaccgta ttcgtc
//
LOCUS       DASYWL010000015         4320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_563584, whole genome shotgun sequence.
ACCESSION   DASYWL010000015 DASYWL010000000
VERSION     DASYWL010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563584"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..1188)
                     /locus_tag="VGR22_00585"
     CDS             complement(4..1188)
                     /locus_tag="VGR22_00585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEV2127097.1"
                     /translation="METSLRKSQARHQLALLAGGLGSWELDLQTMVLESSDSCKANMG
                     LGPHDQISYARLLEVILPEDRPAMQASVQRAVETGGDYQADYRVQWPDGSIHWIAAYG
                     RPFFAADGTPERMLGTTQEVTERKQAEQERERLLREARNARDEAQRAVQLRDTFLSST
                     VHDLRNPLTSMRGRTQLLQRRISKLNLADADRQRLEDGLAQIEVSTGQIQAILAELQD
                     MAFLQIGRPLELKCAPTDLVALVQSAIDQHRLQARRHEVRFDTSHEHLIAAVDPTRIE
                     RVITNLLSNAVKYSPGGGRVTVSLWGDETGVALAVSDEGIGIPAGELGLIFERYRRGT
                     NVTKSFEGTGIGLSGSRQIVEQHGGAISVDSEEGKGSRFTVRLPIDVVHENPPHCTGE
                     NG"
     gene            1385..1810
                     /locus_tag="VGR22_00590"
     CDS             1385..1810
                     /locus_tag="VGR22_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127098.1"
                     /translation="MSNATNQNVTIVAAVPGCELRRSAGGSEKYVLSDMASNISIDLT
                     GERMADSTLQAMVKSLGDNAVTINNEHSSSWDDFDEVTKLWVMPSHELTMEAELTTDH
                     YRSKTLIKGLDNRAPFLAPPRHTSPRYTDRRKGATFAEN"
     gene            1851..2054
                     /locus_tag="VGR22_00595"
     CDS             1851..2054
                     /locus_tag="VGR22_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127099.1"
                     /translation="MSKASQEILRAITQLKKDEVLRMQPDPDKSLRGLKTAVGRVASR
                     NDLKLETWSDEAGDVLYVRKVRS"
     gene            complement(2336..2617)
                     /locus_tag="VGR22_00600"
     CDS             complement(2336..2617)
                     /locus_tag="VGR22_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127100.1"
                     /translation="MSEQFKAALVRALVVAGLGALATWLATWATTDDATVIMAAVAAA
                     FLAAFLARFGGEGAWDTKRARDRDVHPADVGYDELHALRTGRVRIVTEV"
     gene            complement(2938..3231)
                     /locus_tag="VGR22_00605"
     CDS             complement(2938..3231)
                     /locus_tag="VGR22_00605"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV2127101.1"
                     /translation="MPCDSVFAGHGDPGDSPGSGHPHRAFTGKAQVRTNGAALFAAMP
                     NFLDDLLQSLQDGDTLWTEWRWVGTLHDGTRSDRRGVILFGVGGDRIVWGRSI"
     gene            3497..>4320
                     /locus_tag="VGR22_00610"
     CDS             3497..>4320
                     /locus_tag="VGR22_00610"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV2127102.1"
                     /translation="MASTQPPSASASATGTLTFLFTDIEGSTKRWERHPDLMARAVAR
                     HDEIMRVTIAQHNGHVFKTVGDAFCAVFPSAEDALHASVAAQRAIAAEDWGEIGPIRV
                     RMGLHTGEVEERDADYFGPTVNRVARIASAGHGGQTLLSAVTGDHAGERLPPEVRVRD
                     LGKHRLKDLARPERILQLIGAGLPSDFPRLKTVPSPGPGIAAAVLSTLVPLVLFRLTN
                     RSSSDEVSASMFSPLSLYTGLKGLVIELSTLNEYLLLAAGLLLLTIALWIGVARWRA"
BASE COUNT          773 a         1494 c         1333 g          720 t
ORIGIN      
        1 gtcctaccca ttctctcccg tgcagtgggg aggattctcg tggacaacgt cgatggggag
       61 ccgaacggta aacctgcttc cctttccctc ctcgctgtcc accgagatcg cgccaccatg
      121 ctgctcgacg atctgccgag agccggacag cccgatgccc gtaccttcaa agctcttggt
      181 gacgttggtg ccccgacgat agcgctcgaa aatcagtccc agctcgccag ccgggatgcc
      241 aatcccctcg tcactcaccg caagtgccac ccccgtctcg tctccccata gcgacacggt
      301 gacgcgccct ccgccggggc tgtacttgac ggcgttggac aagaggttgg tgatgactcg
      361 ctcgatgcgg gtggggtcga ccgcggcgat caggtgctca tgcgacgtgt cgaaccgaac
      421 ctcgtgtcga cgggcctgca atcgatgttg gtcgatggct gactggacca gggccacgag
      481 gtcggtcggg gcgcacttca actccagcgg gcgaccgatc tgcaggaacg ccatatcctg
      541 cagctccgcg aggatggctt ggatctgccc cgtgctcacc tcgatctgcg cgaggccgtc
      601 ctcaaggcgc tgtcggtccg cgtctgccag attcaactta ctgatgcgac gttggagcaa
      661 ctgggtgcga ccccgcatgc tggtcagcgg gttgcgcaag tcatgtacgg tcgaggacag
      721 aaaggtgtcc cgcagctgca cggcccgctg cgcctcatcg cgggcattac gagcttcccg
      781 gagcagccgc tcccgctcct gctccgcctg tttgcgctcg gtgacctcct gggtggtgcc
      841 cagcatccgc tccggggtcc cgtcggcggc aaagaagggt cgcccgtacg cggcgatcca
      901 gtggatactc ccatccggcc actgaacccg gtagtcggct tgatagtcac cgccggtctc
      961 caccgcgcgt tgcaccgagg cctgcatggc ggggcgatcc tcgggcagga tgacctccag
     1021 cagccgcgcg taggatatct ggtcgtgcgg accaagaccc atattggcct tgcagctatc
     1081 cgagctctcc aacaccatcg tttgcaggtc gagctcccac gagcccagcc cgcccgccag
     1141 aagggcgagc tgatgccgcg cctggctctt gcgcagagac gtttccactc tccgcagtcg
     1201 atccagaatg gttgaggacc gtgtcatgac cgggtgcctc cgatgaccac gatgtggggc
     1261 caggcaaagc agcagatagc ccacatgcta caacaaggtg aatcaacccc gcgtgactcc
     1321 cacgcgagac accatcgagc gtccgaagcg agctgaatga atcagctctc ggacgcttat
     1381 tcgtatgtcg aacgccacga accagaacgt cacgattgtc gctgccgtcc ccggctgtga
     1441 gctccgcagg agcgcgggtg gatccgagaa gtatgtcctc tccgatatgg cctccaacat
     1501 cagtatcgac ctgaccggcg agcgcatggc cgacagtacc ctgcaagcca tggtcaagtc
     1561 gctgggggat aacgccgtca ccatcaacaa cgaacacagc tccagttggg acgatttcga
     1621 cgaagtgaca aagctgtggg tgatgccttc ccacgaactg acgatggaag ccgagctcac
     1681 taccgaccac taccgcagca agacgctgat caaaggtctg gacaatcggg cgccttttct
     1741 cgcaccaccc cggcatacct cgccgcgata tactgaccgc cgaaaggggg caacctttgc
     1801 cgagaattga gacgatcaaa gcgtcggacg caccgccacc accgaagaag atgtcaaagg
     1861 catcgcagga gattctccgg gcaatcactc agctcaagaa ggacgaggtc ttgcgaatgc
     1921 agccggaccc cgacaagagc ctgcgaggtc tcaagaccgc ggtcggccgg gtggcctcac
     1981 gcaacgacct caagctcgag acgtggtccg acgaggccgg ggacgtcctc tacgtgcgca
     2041 aggtgcggtc ctagccaacg gcacagcgtt cgtctcgccg cgtaccgagg gtcaggacgg
     2101 ccatgaacga gtggcgccca tcggttggag cgagcggctg tcggtggccg gtgaagctcc
     2161 ggatggcagg gactgcgcgg gatgtgccgc gcgtcgtcgc gccgggaacc gggccgcatc
     2221 cgcacgccgg gtcaacggct ccggcgcgtt gtcggtcgat cttcgcggtc gctcagtagc
     2281 gtcgagtgcc gtgccactca cggcgcatac acacgcccgc ggtcagcgcg tgcggtcaga
     2341 cctcggtcac gatgcgcacc cgacccgttc gcagggcgtg caactcgtcg tacccgacgt
     2401 ccgcaggatg cacgtcgcgg tccctggctc gcttggtgtc ccacgctccc tctcccccga
     2461 agcgggcgag aaacgccgcc aggaacgccg ccgcgacggc agccatgatc accgtggcgt
     2521 catcggtggt cgcccaggtg gcaagccacg tcgcgagggc accgaggcca gcgacgacga
     2581 gcgcgcgaac caacgcagcc ttgaactgct cggacatgtc ctcgtctccc gatgctgtaa
     2641 tgacgttgca accttaccga ggtagaacac cattgtgtca cgtcaccgac aagaatggaa
     2701 cgaggaataa tcggcaatac acagcaacag gtcgacccgt tgcggcgccg catgacccgc
     2761 agcaacggcc cgtggtgtgg ttgccagcaa tgctcccaca gcacactgtc ctggttggac
     2821 acgctctcag tccgcaatcg gctgatctca ccggcgactg gacgggacgt cgatgtccct
     2881 accgccgaaa cccggccagc gtctggtgga tgcccgcgct ggtcgcctcg ggcggctcta
     2941 tatggagcgg ccccaaacaa tccggtcgcc gcccacgccg aagaggatga cgccccgcct
     3001 gtcgctccgg gttccatcgt gcagcgtccc cacccacctc cactccgtcc acagcgtgtc
     3061 gccgtcctgc agggactgga ggaggtcgtc caggaagttc ggcatcgccg cgaacagcgc
     3121 cgccccgttc gtgcgcacct gggctttgcc ggtgaaggcg cggtgagggt gaccggatcc
     3181 cggcgagtcg ccgggatctc cgtgaccagc gaacacgcta tcgcacggca cgccctcaga
     3241 accccttccg tgaatacacg gcgggggaag gtattgcggg ttttgcgggg atcatccaga
     3301 cgctcgacgc cgcctccccc taccccacag ggagggcggt aatccgagag ggtggccgag
     3361 cgacaccgaa atcgcaacgg cagtcgcctg agatgttccg gaatggcgac ggtggcccta
     3421 caatgtcatc aggaatccat cgcctcgcac catcgggtcc catcttcgtc gcggttctcc
     3481 tcacgagtga gccaccatgg catcgaccca gcccccctcc gcttcggcct ccgcgaccgg
     3541 caccctgacg ttcctcttta ccgacatcga agggtccacc aaacgctggg agcgccaccc
     3601 ggacctgatg gcgcgcgccg tcgcccgcca cgatgagatc atgcgcgtga ccatcgcgca
     3661 gcacaacggc cacgtcttta agaccgtcgg tgatgccttc tgtgccgttt tcccgagcgc
     3721 ggaggacgcc ctccacgcca gcgtcgcggc ccagcgggcg atcgccgccg aggactgggg
     3781 cgagatcggc ccgatccgcg tcaggatggg cctccatacc ggcgaggtgg aggagcgcga
     3841 cgccgactac ttcggcccca ccgtcaaccg ggtggcccgc atcgcctcgg cgggtcacgg
     3901 cgggcagacc ctcctctccg ccgtgacggg cgaccacgcc ggcgagcgcc tcccgcccga
     3961 ggtacgggtg cgcgacctcg ggaagcacag gctcaaggac ctcgcccggc ccgagcggat
     4021 cctgcagttg atcggcgccg ggctgccaag cgacttcccc cggctgaaga cggtcccatc
     4081 ccccggtccc ggcatcgccg ccgcggtgct ctcgacgctg gtgccgctcg tgctcttccg
     4141 gttgaccaat cggtcgtcat ccgacgaggt cagcgcgtcg atgttctcgc cgctctcgct
     4201 ctacaccggg ctcaaggggc tcgtcatcga gctctcgacc ctcaacgagt acttgctcct
     4261 cgcggccggt ctgctcttgc tgacaatcgc tctctggatc ggggtcgcgc ggtggcgcgc
//
LOCUS       DASYWL010000016         1759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_113401, whole genome shotgun sequence.
ACCESSION   DASYWL010000016 DASYWL010000000
VERSION     DASYWL010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113401"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VGR22_00615"
     CDS             complement(<1..237)
                     /locus_tag="VGR22_00615"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEV2127103.1"
                     /translation="MDDSQASEQPQQQGQSGQQAASTEETLRGTFLGIVHDLRNIVRG
                     EAELARAELKEDANRIGKGAGMIAGSGVLSYTGFL"
     gene            586..1752
                     /locus_tag="VGR22_00620"
     CDS             586..1752
                     /locus_tag="VGR22_00620"
                     /inference="COORDINATES: protein motif:HMM:NF025168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HEV2127104.1"
                     /translation="MTDTDTRTHHPSPLPPGTTRPNGSDDIQRMARTIADEPGSIFRV
                     IRDLPGIRSWKILNDLVFLLLSGVLGIFWFVVAVVGFSVGGSLLIILVGFPILAGTIA
                     LLVWGAHLERARIRTFLGIDIPSPYRDMPQEGGRIRHGWRLLRTSAVWRDVAYLLLLF
                     PIGLLEMSVALLPIQFFITPLITLFGGENNIIFWSIDSFGEGIVAVILGAILFVPLSI
                     LINLVATLHGEFARRLLTRSSAEILTERVEELTESRSAVMKAMHLERRRIERDLHDGA
                     QQRLVSLAMDLGRARDRMESGDADGVRELLVHSHEESKVILKELRDLVRGIHPAVLSD
                     RGLDAAISAIAGRSSIPVEVDVDLANRLPDEVEGTAYFFVVEALANIAKHNHAT"
BASE COUNT          269 a          672 c          478 g          340 t
ORIGIN      
        1 gaggaagccc gtgtagctga gcaccccgct tccggcgatc atgcccgctc ccttcccgat
       61 ccggttcgcg tcctccttca actcggccct ggccagctcg gcctcgccac gcacgatgtt
      121 ccgcaaatca tgcacgatac cgaggaatgt gcccctcagc gtctcctccg ttgatgcagc
      181 ctgctggccg gattgaccct gctgctgagg ctgctcgctt gcttggctgt cgtccatctg
      241 catgcctcct ttctcaagct cgccgtgggc gtctctgaga tccccggcgc ggttggcacg
      301 aatccgagca agaatcgttc ccgtaacctc tcggcgcccg tccaccgccc cccggcatcg
      361 ccgatgggca gcggccgatg ggagggataa ccctccctcg cgattcgggg tgcgccacct
      421 gtttctcgct ccccgaccag ccgatcatgg ttgcggggcc atcggtttcc tggccctgcg
      481 aacaccgcag cctcgaagac atcggcaccg cgattccgcg ctaccgctcg ctacactgga
      541 tacagcggcc ctcagccggc tccagccggc agacaggatc ctcacatgac cgataccgat
      601 acccgcactc accacccatc cccgctcccg cccggcacca cccgtccgaa cggcagcgac
      661 gacatccagc gcatggctcg caccatcgcc gacgagcccg gctccatctt ccgggtgatc
      721 cgcgatctcc ctggcatccg ctcctggaag atcctcaacg acctcgtctt cctcctgctc
      781 agcggcgtgc ttggcatctt ctggttcgtg gtcgcagtcg tcggcttttc ggtcggcgga
      841 tcgctcctca tcatcctcgt cggcttcccc atcctcgccg gcaccattgc cctcctcgtc
      901 tggggtgcgc acctggaacg cgcccgcatc cgcaccttcc tgggcatcga tatcccctcg
      961 ccatatcgag acatgccgca agagggtgga cgcatcaggc acggctggcg gttgctgcgt
     1021 acatccgccg tctggcgcga cgtcgcctac ctcctcctgc tcttccccat cggactgctg
     1081 gagatgagcg ttgcgctgct cccgatccag ttcttcatca cgccgctcat caccctcttc
     1141 ggcggtgaga acaacattat tttctggtcg atcgacagct ttggcgaagg aatcgttgcg
     1201 gtcattctcg gcgcgatcct gttcgtgccg ctcagcatcc tcatcaacct ggtggcgacg
     1261 ctccacggcg agttcgcccg gcggctgctc acccgctcca gcgccgagat tctcaccgag
     1321 cgcgtcgagg agctcaccga gagccgatcc gcggtcatga aggcaatgca cctggagcgc
     1381 cgccgcatcg agcgcgacct ccacgacggt gcccaacagc gcctcgtctc cctcgcgatg
     1441 gacctcggtc gcgcccgtga ccgtatggag tccggcgacg ccgacggcgt ccgcgagctc
     1501 ctcgtccact cccacgagga gtccaaggtc attctcaagg agttgcgtga tctcgtccgc
     1561 ggcatccacc ccgccgtcct gagcgatcgc ggtctcgacg ccgccatctc ggcaatcgcc
     1621 ggacgctcct cgatccctgt cgaggtcgac gtcgatctcg ccaaccgcct gcccgacgag
     1681 gttgagggca ccgcatactt cttcgtcgtc gaggcgctgg cgaacatcgc caagcacaac
     1741 catgccacct aggcacacg
//
LOCUS       DASYWL010000017        12250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_563606, whole genome shotgun sequence.
ACCESSION   DASYWL010000017 DASYWL010000000
VERSION     DASYWL010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 12250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12250
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563606"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VGR22_00625"
     CDS             <1..548
                     /locus_tag="VGR22_00625"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV2127105.1"
                     /translation="IHAAGRLVAQLDALALPANPKTTLNVGILEGGISVNTIAPEARM
                     VIDMRSLEQESLEDLVREVQGQIDAVATDDLHVKTTVVGDRPAGRLPADQGLVPIARE
                     ALALVGIEASFDASSTDANIPISRGIPAVCIGVTSGGNAHREDEYIDLELVPTGVTQL
                     AVVTLEASRLLAEDRLTIVTE"
     gene            582..3530
                     /locus_tag="VGR22_00630"
     CDS             582..3530
                     /locus_tag="VGR22_00630"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HEV2127106.1"
                     /translation="MLRLAEEHALDSQVFVALDVEGTGMDPAVDEIIEIAIVRFTRQE
                     VLDSWSTLMRPSHKPGTEIVHLTGIQPQELAEAPRFAEVSAVVREKLGAWPLVGHTVT
                     SDIDMLESAGLRLANRSLDTHRLSTLFLHGLPAYSLAAVADALGVHTQNVHRALVDAD
                     AARRVFLSIIRLMQRYSTTTLNHAARYIEGAGWSEAWLLQLMANDDTLSPVFRTDSDL
                     PWLEPAETRFLSRLQRPDALKKTGVTTPIGVDDVERALTAGGALGEVLDHFETRPGQI
                     EMASAVTEAFNHDAMLMVEAGTGTGKSMAYLLPAALHAIKRGERVVISTDTRALQEQL
                     YQKDVPDVQSAVDRLGIPDDVRAAVLKGRTNYLCLRRWFSHDRREPSDGADAAMRAKV
                     TVWLDETDTGDQAELRLTPDEQGHWRQIAAEEEACVASQCPFNQRNQCFLYRAKRKAE
                     HAHLVVANHSLLLADTDHRVLPEFDRLVIDEAHHLEDEATRHFGYVLEQRVIEALLDS
                     LLQGRRTGQRGTLEQVATFLSGSAESSARKAAPEARERVQNVSSIRARVLGLTTELFG
                     RIDRMLRDQRAGGGYGASLRITDAARSRQPWLELEQIWEQLDRGLFDYEALVTWLLNE
                     LERLPSPREGTDDPQSLVREELWLELMSRWRELHEFRHRLTGAIAEPAEDTIYWLEQV
                     GPQRVPTLHAAPLFVDELLHQKLFQDMRTVVATSATLTIDSSFEFMASRTGLRNADAV
                     SVGSPFDHEASTLLLLPDDMPFSNESRYQYALNDSIVRLCTASGGRALVLFTSYAALR
                     ASYDSVKAALQRHDIAVYAQGVDGGAKALVDRLKATDRSVIFGTSSFWEGIDVPGEAL
                     SMLIITKLPFPVPSDPVFQARGELLDNEFIQLAVPRAVLKFKQGFGRLIRRATDKGVC
                     AVLDRRVIAKRYGAHFLHSLPPTTQRICSRTDMASLAGMWLDDRPLPSVPDLFGDPFD
                     SFDVHEGVWR"
     gene            3527..4510
                     /gene="glpX"
                     /locus_tag="VGR22_00635"
     CDS             3527..4510
                     /gene="glpX"
                     /locus_tag="VGR22_00635"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEV2127107.1"
                     /translation="MTKPITRNLALELVRGTEAAALMAARWMGRGEKEAADGAAVDAL
                     RDVLNSIEMDGVVRIGEGDKDEAPMLYTGEQVGRGTEPKVDIAVDPVEGTRLLAQGMP
                     NALVILAVAERGSLYGWQDIAYMDKIAVGPDAAGAIDINASVAENLHSVAEAKGADVS
                     DLTVVILDRPRHEQLIREVREAGARMKLITDGDVAGGLMAAMPGTGIDMLMGIGGAPE
                     AVITACALKCVGGDIQTKLWVRNEQEEATVRERNLDVNRVYRLSDLVQSEDTFFVATG
                     ITDGELLRGVHYFADGATTHSLVMRERSGTVRYIESSHRISKLRELADSRF"
     gene            4956..6563
                     /gene="rho"
                     /locus_tag="VGR22_00640"
     CDS             4956..6563
                     /gene="rho"
                     /locus_tag="VGR22_00640"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEV2127108.1"
                     /translation="MSIDEVVTEAPQAEEEAKPARKPRVARRKKDLEAVAEETAAQET
                     ADNDHPAAEALATTDLRPGGDAAPPPTEQADDEEQRATQGAAEGKRGRGKNRRTRGKK
                     SPDGETEQRGGQNQNGNRITVAELNAKPDEDLWALARELEVSGYMRMRRPDLVTKLLQ
                     AQTEKAGHKFGDGVLEIIEDGFGFLRGQRFLPGSNDIYVSQSQIRRFGLRTGDRVSGQ
                     VRPPKDNEKFFSLLRVESVNGIDPETARRRPSFDTLTPIFPLEQIHLETQPNILSTRL
                     LDLVAPIGKGQRGLVVSPPKAGKTLLLKSIANGITSNNPDMHLMVCLIGERPEEVTDM
                     RRSVDGEVISSTFDEPVEDHTKVAEMALERAKRLVESGRDVTILLDSITRLARAYNLA
                     VPPSGRTLSGGIDPIALYPPKRFFGAARNIEGGGSLTIISTCLIDTGSRMDDVIYEEF
                     KGTGNMELHLDRKLAERRIYPAIDIQRSGTRREELLLGEDVLRQVWTMRRMVSMLGGT
                     EGTELVLSRLSKTDTNSDFLQTLHKDM"
     gene            6900..8012
                     /locus_tag="VGR22_00645"
     CDS             6900..8012
                     /locus_tag="VGR22_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEV2127109.1"
                     /translation="MTLDKSAATSPATMSREDRQLWFLAGLRQAMADKGHAVADSSAG
                     EASEASRIVINPIHPERPKSYRRRSRTIFVVGLAALDGLPEDILREGYTLLVRSMSNV
                     FMLLLEDEEGLAVYFITMERGFYSVRYGGDDAAFFDAVAERLIPLATSRLVIDNVFTP
                     DLPEALWNGDEHTEAIYRAGVRLAELDLLPAAFPLEELLGPRDLRHLHRLFGIGGLSY
                     GNVSARLDEETFWMSASGVDKSHLCDVGTEVLLVAGFDEENQAIHLSVPPNVTPRRVS
                     VDAIEHYMLYREHPGVGAILHVHSWMNGIPSTEVNYPCGTIELAQEVAELVREADDPN
                     RAVIGLKNHGLTITGPSLDEIFDRVESRLLHQIPMS"
     gene            8015..9262
                     /locus_tag="VGR22_00650"
     CDS             8015..9262
                     /locus_tag="VGR22_00650"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV2127110.1"
                     /translation="MGNAMDASTRGLRYRLSIPNYLAVRAMDNLLPLAMSSGRIPGLS
                     YTATPPLELPGPDWLRLRPLLSGICGSDMALLTGKSSPALSPFTSFPAVLGHEVLAEV
                     TECGAEVRGVNVGDRVVIDPFISCEMRGLEPCPSCRTGQRCVCTQTAEGSLSPGMLVG
                     FCRDLPGGWSGEMIAHRGQVYCVPAEIPDDVAVLVEPFSVALHAVLKLVPEPRSRVLV
                     LGGGTVGLLVLSALRLLKLDCHVTVIARYAVQAEMARRLGADAVHEQAGAAAIDVTGA
                     SRYKPLKGRPVYTGGFDTVYDCVGSGRSLDESMRVAGPQGRAVIVGCVGEVSRLDMTF
                     VWARELYVTGSYGYAMEHDLEGKPHTFDLALDLLRGNSDYPLGELVTHRFPLSSWLEA
                     MRVNLRRSQSGAIKTIFDCREVG"
     gene            complement(9348..10295)
                     /locus_tag="VGR22_00655"
     CDS             complement(9348..10295)
                     /locus_tag="VGR22_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HEV2127111.1"
                     /translation="MNQGTVVASSNGMVGLPAAIEVLRNGGSALDAAVAGTKIVEANP
                     EDHTVGYSGLPNMLGDVELDASVMEGHGLKAGSVGALTGYQDAVELARKVMETLPHVL
                     IAGDGARQFARELEFEPKELLTPEAEAIWRKRIDDEADRDASYMGKVRELVAASASDP
                     DYADLGEPPHGTVNFIARDRNGEIACAVSTSGWAWKYPGRMGDSPIIGAGNYADNRWG
                     AAACTGRGEMAQRCLTAHSVVMFMRFGMSLDEALDQAVRDLDHLDDPFRSEMNIVALD
                     KHGNPGAASTAKDKTYAMMTEEMEQPEERPRMHVPIAGT"
     gene            complement(10292..10570)
                     /locus_tag="VGR22_00660"
     CDS             complement(10292..10570)
                     /locus_tag="VGR22_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEV2127112.1"
                     /translation="MNQPEIIVYARARFSPDAQRTRDRLLELDLSYVEHDIETDVVAG
                     EALEELTGVRRVPTVVIGDRVLIEPSTEELDVALRDAGFTTPPSRREQ"
     gene            complement(10585..11619)
                     /locus_tag="VGR22_00665"
     CDS             complement(10585..11619)
                     /locus_tag="VGR22_00665"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase family protein"
                     /protein_id="HEV2127113.1"
                     /translation="MSRNPSPLQPGKLPAAFLADLLASHVTVDDPSVLVAPAPGFDAA
                     VVRPRHDIIVKSDPITFTTSKPAAYLVAVNANDIACLGGVPRWLTVTALFPEGTTAVD
                     VAEIFDTLADACQRIGISLIGGHTEVTSAVRQLVLSGTMVGVPGPQGTLAPGGARPGD
                     EVWITQAAGIEGTSIMATEVPAQHLRGISDWALEVGRSLIDDPGISIVEAAQTARSAG
                     PVTAMHDPTEGGVATALHELADASRLGIEVNIEAVPVWPVTRALADHFGISPLGLISS
                     GALLFTAPPGSGEAFQAAFETTGIPVSRIGTMLADPNVRVARAAGSATELPRYDTDEI
                     TSVFARIEAH"
     gene            complement(11636..11857)
                     /gene="xseB"
                     /locus_tag="VGR22_00670"
     CDS             complement(11636..11857)
                     /gene="xseB"
                     /locus_tag="VGR22_00670"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEV2127114.1"
                     /translation="MNATDTSALEEPSFEERLEELKTLVEELEAGDLSLEDMLQRYEQ
                     GMSLIATCQDRLERAQLRITEIAASGDDR"
     gene            complement(11854..>12250)
                     /locus_tag="VGR22_00675"
     CDS             complement(11854..>12250)
                     /locus_tag="VGR22_00675"
                     /inference="COORDINATES: protein motif:HMM:NF014640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEV2127115.1"
                     /translation="IEIAERRLANEWSQLDALTRRFQQQSPRAHISRTGQELVTLERR
                     LRLSGRSYLQQRETSLRSAAKVLAALDPTRVLERGYAVIETPAPRTPIRRVADLDGDT
                     AVVVNFRDGTARGTIAPHTNDSQEDAQQS"
BASE COUNT         2165 a         3781 c         4077 g         2227 t
ORIGIN      
        1 ccatccatgc cgccggccgg ctggtcgcgc agctcgacgc gctcgcgttg ccggcgaacc
       61 ccaagaccac cctcaatgtc ggtatccttg agggcggcat cagtgtcaac accatcgcgc
      121 ccgaggcgcg catggtgatc gacatgcgct cgctggagca ggagtcgctt gaggatctgg
      181 ttcgcgaggt ccagggccag atcgatgccg ttgcgaccga cgacctccat gtgaagacca
      241 cggtcgtcgg cgatcggcca gccggtcgcc tgccagccga tcaggggctc gtgccgatcg
      301 cgcgggaggc gctcgcgttg gtcggcatcg aggcgtcgtt cgacgcctcg agtaccgacg
      361 ccaacatccc gatcagccgc ggcattcccg ccgtctgcat cggtgtcacc tccggcggca
      421 acgcccaccg tgaggacgag tacatcgacc tcgagctggt cccgacaggc gtcacccagc
      481 ttgccgtggt gaccctcgaa gcctcacggt tgcttgccga ggatcgattg acgatcgtca
      541 ctgaatgacc ggagtaccgg tcctggaaag tccgttgcct catgcttcga ctcgctgaag
      601 agcacgcact cgacagccag gtcttcgtgg cgttggatgt cgagggcact ggtatggacc
      661 ctgccgtcga cgagatcatc gagatcgcga tcgtcaggtt cacgcgacag gaggtgctcg
      721 acagctggtc gacgctgatg cggccgtccc acaagcccgg taccgagatc gtgcatctga
      781 ccgggataca accgcaggag ttggccgagg cgccgcgctt cgccgaagtc tccgccgtag
      841 ttcgagaaaa gctgggggcg tggcccctgg tggggcacac cgtcacatct gacatcgaca
      901 tgctggagtc ggccgggttg cgactggcga atcggtccct ggacacgcat cgcctcagca
      961 cgctctttct ccacgggctg ccggcctaca gcctggctgc agtggcagac gcgctcggcg
     1021 tgcacaccca gaacgttcac cgagcgctgg tggatgcgga tgcggcccgc cgggtcttcc
     1081 tctccatcat tcggctcatg caacgctatt cgaccacgac tttgaaccac gccgcccgct
     1141 acatcgaagg cgcagggtgg agcgaggcgt ggctcctgca attgatggcc aacgacgata
     1201 cgctgagccc ggtctttcga accgatagcg acttgccgtg gctcgaaccg gccgagacgc
     1261 ggtttctctc gcgcctgcag cggcccgacg cgctcaagaa gaccggcgtg acgacaccga
     1321 tcggggtcga tgatgtcgag cgtgcgctga cggctggtgg tgcgctcggc gaggtcctcg
     1381 accacttcga gacacgaccc gggcagatcg agatggccag cgctgttacc gaggcgttca
     1441 atcacgacgc aatgctgatg gtcgaggcag gaaccggcac cggcaagagc atggcttacc
     1501 tgctgccggc ggcgctgcac gcgatcaagc gcggggagcg tgtggtcatc tcgacagata
     1561 ctcgtgcctt gcaggagcag ctctaccaga aggacgtccc ggatgtgcag tcggcggtcg
     1621 atcggctcgg catccccgat gacgtgcgag cggcggtgtt gaagggtcgc accaactacc
     1681 tctgcctgcg ccgctggttc tcgcacgacc gccgcgagcc atccgacggc gccgacgcgg
     1741 ccatgcgcgc caaggtgacg gtctggctcg acgagaccga caccggcgac caggccgagc
     1801 tccgactcac gcccgatgag caaggacact ggcggcagat cgccgccgag gaggaggcat
     1861 gtgtcgccag tcagtgcccg ttcaaccagc gtaaccagtg tttcctctat cgggcgaagc
     1921 gcaaggcgga gcacgcgcac ttggttgtcg ccaaccactc gctgctgctt gccgataccg
     1981 atcatcgtgt cctgcccgag tttgatcgtc tggtcatcga cgaggcacac catctcgagg
     2041 atgaggcgac caggcacttc ggctacgtgt tggaacagcg ggtgatcgag gcgctgctgg
     2101 acagcctgtt gcagggcagg aggacgggcc agcgcgggac actcgagcag gtggcgacat
     2161 tcctttccgg atcggcggag tcaagtgcca gaaaagccgc accggaggct cgggaacggg
     2221 tccagaacgt aagcagcatc cgggcgcgag tcctgggtct caccacggag ctctttggac
     2281 ggatcgaccg catgctgcgg gaccagcgtg cgggcggtgg ctacggcgct tcactccgaa
     2341 tcactgacgc ggctcgctcg cgtcagcctt ggctggagtt ggagcagatt tgggagcaac
     2401 tcgaccgcgg cctctttgac tacgaggcgc tggtgacttg gctgctgaac gagctggagc
     2461 gcctaccatc gccgagggag gggacggacg acccgcagtc gctcgtgcgg gaggagctct
     2521 ggctggaact gatgtcccgc tggcgagagc tgcacgagtt ccggcaccgg ctgaccgggg
     2581 cgatcgccga gccagccgag gacaccatct actggctgga acaggtcggg ccgcaacggg
     2641 tgccgaccct gcacgcagcg ccgcttttcg tcgacgagct gctgcaccag aagctcttcc
     2701 aagacatgcg gacggtggtt gcgacttccg cgacgctcac catcgacagc agcttcgagt
     2761 tcatggccag tcgcacgggc ctccggaatg ctgacgcggt ctcggtcggg tcgccgttcg
     2821 accacgaggc gtctaccctc ctgttgttgc ccgacgatat gccgttctcg aatgaaagcc
     2881 gctatcagta cgcgctgaac gacagcatcg ttcggctctg cactgcgagc ggcggccgtg
     2941 cactcgtgct cttcacctcg tatgccgcac tgcgagcgtc gtacgattcg gtcaaggcgg
     3001 cactccagcg gcatgatatc gccgtctatg cgcagggagt ggacgggggc gccaaggcac
     3061 tggtcgatcg cctcaaggcg actgatcgca gcgtgatctt cggtacatca tccttttggg
     3121 agggaatcga cgtcccgggc gaggcgctca gcatgctcat tatcaccaag ctgcccttcc
     3181 cggttccgag cgatcccgtg ttccaagcgc gcggcgagct gctcgacaat gagttcattc
     3241 agctggcggt gccgagggcg gtgctgaagt tcaaacaggg gttcggccgg ctgatccgac
     3301 gagcgacgga caagggcgtc tgcgcggtgc tcgaccgccg cgtgattgcc aagcgatatg
     3361 gcgcacactt cctgcattcg ctgccgccga ccacccagcg tatctgtagc cgtaccgaca
     3421 tggcgtcgct tgccggcatg tggctggatg atcgaccctt gccgagcgtg cctgacctct
     3481 ttggcgatcc gttcgattcg ttcgatgtgc atgaaggagt ttggcgatga cgaagccgat
     3541 tactcgcaac ttggcgctgg agctcgtacg tggcaccgaa gccgcggccc tgatggcagc
     3601 ccgctggatg gggcgtgggg agaaggaggc cgcggacggc gcggcggtcg acgcgctgcg
     3661 ggacgtgctc aattcgatcg agatggatgg cgtcgtcagg atcggtgagg gcgacaagga
     3721 cgaggcgccg atgctctaca ccggggagca ggtcgggcgg ggcaccgagc caaaggtgga
     3781 tattgccgtc gatccggtcg agggcacacg gctgcttgca caaggcatgc cgaacgcgct
     3841 cgtgatcctg gcggtagccg aacgaggctc cctctacggg tggcaggaca tcgcgtacat
     3901 ggacaagatc gctgtaggac cggacgcagc cggcgcgatc gacatcaacg cgtctgtggc
     3961 ggagaacctg cacagcgtag ccgaggcaaa gggggccgac gtttccgacc tgacggtcgt
     4021 cattctcgat cggccccgcc atgagcagct gattcgtgaa gtccgggagg cgggtgcccg
     4081 tatgaaactc atcaccgacg gagatgtcgc gggtgggctg atggcagcca tgccgggcac
     4141 cggcatcgat atgctgatgg gaatcggagg agctccggaa gcggtgatca ccgcctgcgc
     4201 cctcaagtgc gttggcggtg acatccaaac gaagctgtgg gttcgcaacg agcaggaaga
     4261 ggcaactgtc agggagcgca atctcgacgt caaccgggtg taccggttga gcgatctcgt
     4321 gcagtcggag gacaccttct ttgtggctac cggcatcacg gatggtgagc tcctccgcgg
     4381 tgttcactat ttcgctgacg gcgctaccac ccactcgctg gtgatgcgag agcggtcggg
     4441 aaccgtgcgg tacatcgagt cgtcgcaccg aatcagcaaa ctccgcgagt tggctgactc
     4501 tcggttctaa cctgcaagtc ccatctggac gaggcgtggt ggggttgggc gatccggcga
     4561 cccccgtatg caggctgtgt gactggcgct attgacgcgg ttttctccgg ggtggtacat
     4621 ttctgcataa cgacatggca acacacacga tgctccacct gccggtcgtc tgccagcacc
     4681 ctatcgacgc gccaactccg agtctccgca tgacggtgtg gcgtggtgcc tggcaccagg
     4741 tatctccctg acacggacat gaagatctca gccccttcac caaggtgctg ccccacctgc
     4801 cgagctcatc tgacccgccg tgaacgggtc gtttacctct ccacaaccct cgcgcaaccg
     4861 cgctgatcgg gcacgttggt ccggtcaatg ccgcctggcc gatttcgcgc atctgaatat
     4921 tcccagtgca aacagttgaa tcggaggatt ccatcttgag tatcgatgaa gttgtgactg
     4981 aagctcctca agccgaggag gaagcgaagc cggcgcgcaa gccgcgcgtt gctcgccgca
     5041 agaaggacct ggaggctgtc gccgaggaaa cggctgcgca ggaaacggcc gacaacgacc
     5101 atccggcagc agaggcgctc gccaccaccg atctgcgtcc cggcggtgat gccgctcccc
     5161 cgccgaccga gcaggccgac gacgaggagc agcgggcaac ccagggcgcg gcggaaggca
     5221 agcgcgggcg cggcaagaac cgccgtacgc gcggcaagaa gtccccggac ggtgaaaccg
     5281 agcagcgcgg gggccagaac cagaacggca acaggatcac ggtcgcggag ttgaacgcca
     5341 agccggatga ggatctctgg gccctcgcgc gggagctgga agtatccggc tatatgcgca
     5401 tgcggcgccc ggacctcgtg acgaagctgc tgcaggcgca gaccgagaag gccgggcaca
     5461 agttcggcga cggtgtgctg gagattatcg aggatgggtt tgggttcctg cgtgggcagc
     5521 gcttcctgcc gggatcgaac gacatctatg tctcccagtc gcaaatccgt cgcttcggcc
     5581 tgcgcacggg cgaccgtgtc agcggccagg ttcgcccgcc gaaggacaac gagaagttct
     5641 tcagcctgct gcgcgtggag tcggtcaatg ggatcgaccc ggagaccgcg cgccgccgtc
     5701 cgtcgttcga cacgctgacg ccaatcttcc cgctggagca gattcatctc gagacccagc
     5761 ccaatatcct ctcgacgcgg ctgctcgacc ttgttgcgcc gatcgggaag ggccagcggg
     5821 gtctggtcgt ctcgccaccg aaggctggca agacactcct gctgaagtcg atcgccaacg
     5881 ggatcaccag caacaatcct gatatgcatc taatggtctg cctcatcggt gagcggccgg
     5941 aagaggtgac cgacatgcgg cgctctgtcg acggcgaggt tatttcctcg acgttcgacg
     6001 agcctgtcga ggaccacacc aaggtcgccg agatggcgct ggagcgcgcc aagcggctcg
     6061 tggagagcgg tcgggatgtg acgatcctgc tcgactcgat cacccgtctc gcgcgtgcct
     6121 acaacctcgc cgtgccaccc agcggccgca cactctccgg tggtatcgac ccaattgcgc
     6181 tctacccgcc gaagcgcttc ttcggcgcgg cgcgcaacat cgagggcggt ggcagcctca
     6241 cgatcatctc cacctgcctg atcgataccg gcagtcgtat ggacgacgtg atttacgagg
     6301 agttcaaggg caccggcaac atggagttgc acctggaccg caagctggcc gagcgccgga
     6361 tttacccggc gatcgacatc cagcggtcgg gtacccggcg cgaggaactg ctccttggcg
     6421 aggatgtgct gcggcaggtc tggacgatgc gacgcatggt gtccatgctt ggcggcaccg
     6481 aaggtaccga gctggttctg agccgtctct cgaagacgga caccaactcg gacttcctgc
     6541 agaccctgca caaggacatg taggtgtcca tgtgccgcaa tgagggcccg agcggtaact
     6601 gtcgctcggg ccctcgttgt ctccctcgcc cggcccgcct gcgaccggga ctggctgcct
     6661 cgtcgcccct cagtgcgcaa gtggcagcct gatcctgcgc ctggtcgcaa gctggctcgg
     6721 tgacggccgg ggcaggcaac cgtcagaatc agccggcaat gccgggctcg taacgcgaaa
     6781 tcagtggccg atgctgccga cggttcaggt ggtatcgtgg agagagacag gcgagaacgg
     6841 cgcaggctga tatcctacgc aatggggtgc gcttgctatt ggggtacgga ggaggaaaca
     6901 tgacgctcga caagtcggct gccacatcgc ccgctaccat gagccgtgag gatcgacagc
     6961 tttggtttct tgccggactg aggcaggcaa tggctgacaa gggacacgcg gtggccgata
     7021 gctccgctgg tgaggccagc gaagcgtctc gaatcgtcat caacccgatc catccggagc
     7081 ggccgaagtc ctatcggcgt aggtcgagga caatatttgt tgtgggactg gcagcactgg
     7141 atggattgcc ggaggacatt ctcagggagg gatacacact cctggtacgg tccatgtcca
     7201 acgtgttcat gctgctcttg gaggacgaag aggggcttgc tgtctacttc atcaccatgg
     7261 agcgcggctt ctacagtgtc cgctacggag gcgacgacgc cgcattcttc gacgccgtcg
     7321 cagagcggct cattccgctt gctacatcac ggcttgtgat cgacaacgtg tttactcccg
     7381 atttgccgga agcgttgtgg aacggtgacg agcatacgga ggcgatctac cgagcgggag
     7441 tgcgactggc ggagctcgat ttgctgccag ccgcgtttcc gctcgaggag ttgctgggcc
     7501 cccgcgatct gcgacatctg catcgcctgt tcggcatagg agggctgagc tacggcaacg
     7561 tgagcgcccg cctggatgaa gagacgttct ggatgagcgc gagcggtgtt gacaagagcc
     7621 atctgtgcga cgtcggcacg gaggtactgc tggtcgccgg attcgatgag gagaaccagg
     7681 cgatccacct gagcgttccg cccaacgtga caccgaggcg cgtgtcggtc gacgccattg
     7741 agcactacat gctctatcgg gagcatcccg gtgtcggtgc gatcctccac gtccattcct
     7801 ggatgaatgg tatcccgtcc actgaggtca actatccgtg tggaacgatc gagcttgctc
     7861 aggaggtcgc cgaactggtg cgcgaagccg acgatccgaa ccgtgccgtc attggcctga
     7921 agaaccacgg cctgacgatc acggggccga gcctggacga gatcttcgat cgcgtggaga
     7981 gccggttgct tcaccaaatc ccgatgagct agttgtgggt aacgcgatgg acgcctcaac
     8041 gcgtgggctc cgctaccggc tttcgattcc gaactatctc gcggtccgcg ccatggacaa
     8101 tctgctgccg cttgcgatgt cgtctgggcg cattccgggt ctgtcgtata cggcaacgcc
     8161 accgctggag ttgccgggtc ccgactggct gcggctgcgc ccgcttctga gtggcatctg
     8221 tgggagcgac atggcgctcc tcacggggaa aagcagtccg gcgctctcgc cgttcacctc
     8281 gtttccggcg gtccttgggc acgaggtcct tgccgaggtg accgaatgcg gtgccgaggt
     8341 tcgtggagtg aacgtgggag accgtgtcgt catcgatcca ttcatctcat gcgagatgcg
     8401 cgggctggag ccctgcccct cgtgccgcac cgggcagcgc tgcgtgtgca cgcagacggc
     8461 cgaggggtca ctctctccgg gcatgctggt cgggttctgc cgtgacttgc ccggagggtg
     8521 gtcgggagag atgattgccc atcgcggcca ggtctactgc gtgccagctg aaatccccga
     8581 tgatgtcgct gtcctggtcg agccgttcag tgttgcactg catgccgttt tgaagctggt
     8641 gccggagccg agaagccgcg tgttggtcct gggaggcggc acggtgggat tgctggtgct
     8701 gtcagcgctg cggctcctga agctcgactg tcatgtgaca gtgatcgcca gatatgcagt
     8761 gcaggccgag atggcgagaa gactcggagc tgatgccgtg cacgagcagg cgggagctgc
     8821 cgcaatcgac gtgacgggtg cgagcagata caagcccttg aaggggcggc cggtctatac
     8881 aggtgggttc gacaccgtct acgactgtgt cggctccggg cgcagtctcg atgagagcat
     8941 gcgggtcgct gggccacagg gcagggcggt tattgtcggg tgcgtcggtg aggtctcacg
     9001 actcgacatg acctttgtct gggcccgtga gctgtacgtc accggaagct acggttatgc
     9061 gatggagcat gatctggagg ggaagcccca tacgttcgat ctggccctcg atctgctcag
     9121 ggggaattcc gattacccct tgggcgaact cgtgactcac cgcttcccgc tctcgagctg
     9181 gttggaagcg atgcgcgtca atctgcggcg cagccagtct ggtgcgatca agacgatctt
     9241 cgactgccgg gaggttggat agcgccgagc cggcctcgac aagtgatgcc cgcggcgcaa
     9301 acgctgagcg atacagagcc aactccagcg agtggacggt ctggtcgcta cgtgccggcg
     9361 atggggacgt gcatgcgagg acgctcctcc ggctgctcca tttcctcggt catcatggcg
     9421 taggtcttgt ccttcgccgt cgacgccgca cccgggttgc catgcttgtc gagtgcgacg
     9481 atgttcatct cgctcctgaa tggatcgtcg agatggtcga ggtccctcac tgcctggtcg
     9541 agcgcctcgt caagtgacat cccaaaccgc atgaacatca cgacgctgtg ggccgtgagg
     9601 cagcgctgcg ccatctcgcc acggcccgta cacgccgccg cgccccaccg gttgtcggcg
     9661 tagttaccgg cgccgatgat cggcgagtca cccatccgcc cgggatactt ccacgcccag
     9721 ccggaggtcg agacggcgca ggcgatctca ccgttgcggt cgcgcgcgat gaagttgacg
     9781 gtgccgtgcg gcggctcccc gagatcggcg tagtctgggt cactggcact ggcggcaacc
     9841 agctcgcgga ccttgcccat gtacgacgcg tcacggtcgg cctcgtcgtc gatgcgcttg
     9901 cgccagatcg cctcggcttc gggcgtcagc agctccttcg gctcgaactc gagctccctg
     9961 gcaaactgac gggccccgtc gccggcgatg agcacgtgtg gaagcgtctc catcaccttg
    10021 cgggcgagct cgacggcgtc ctggtatccc gtaagcgcgc cgacggaacc ggccttgagt
    10081 ccatgaccct ccatcacgct tgcgtcgagc tccacgtcac cgagcatatt cggcagtccg
    10141 gagtagccaa cggtgtggtc ctccggattt gcctcgacga tcttcgttcc ggcaacggct
    10201 gcgtcgagtg ctgatccgcc attgcgcagc acctcgatcg cggccggcag gccgaccatg
    10261 ccgttggagg acgccacgac ggttccctga ttcattgctc tctcctgcta ggtggggtag
    10321 tgaagcctgc gtcgcggagc gccacatcca actcctccgt ggagggttcg atcagtacgc
    10381 ggtcaccgat cacgaccgtc ggaacacgcc gcacgccggt caactcctcg agggcttcac
    10441 cagcgaccac gtccgtctcg atgtcgtgct cgacgtacga aaggtcgagc tccaggagcc
    10501 ggtcacgggt gcgctgtgcg tctggcgaga agcgcgcgcg ggcgtagacg atgatttcgg
    10561 gttggttcac cggcggaaga ctcctcagtg ggcttcgatt cgggcgaaaa cagacgtgat
    10621 ctcgtcggta tcataccgtg gcagctcggt ggccgagcct gcggcgcggg ccacgcggac
    10681 gttcggatct gcgagcatcg ttccgatccg gctgaccggg atgccagtcg tttcgaacgc
    10741 cgcctggaac gcctcgccgc tgcccggcgg tgccgtgaag agcaacgccc cggaggagat
    10801 gagcccaagt ggggagatgc cgaagtgatc ggccagcgcg cgagtcaccg gccaaactgg
    10861 gacggcttcg atgttgacct cgattccgag gcgtgacgca tcggcgagtt cgtgcagcgc
    10921 tgtggcgacg ccgccctcgg ttggatcgtg catggctgtg accggaccgg cagagcgggc
    10981 ggtttgcgct gcttcgacga tcgagatgcc cgggtcgtcg ataagacttc gtccgacttc
    11041 gagagcccag tcggagatgc cacgaagatg ttgggcagga acttccgtcg ccatgatgga
    11101 ggttccctcg ataccggctg cctgggtgat ccagacctca tcaccgggac gcgcgccacc
    11161 cggtgcaagc gtcccctgtg gcccaggcac accaaccatc gttccggaga gcaccagttg
    11221 gcgaaccgcc gaggtgactt ccgtgtgccc gccaatgagc gagataccga tacgctggca
    11281 ggcatcggcg agcgtatcga aaatctccgc gacatcaact gcggtcgttc cctcgggaaa
    11341 gagtgcggtc acggtcagcc agcgcggtac tccgccgagg caggcgatgt cgttggcgtt
    11401 gaccgcgaca aggtaggctg cgggcttcga cgtcgtgaac gtgatcggat cgctcttgac
    11461 gatgatgtcg tgacgcggcc gcacaacggc ggcgtcgaag cctggggccg gtgcgacgag
    11521 caccgagggg tcatcaacgg tgacatggga ggcgagcagg tcggcgagaa atgccgccgg
    11581 gagcttgccg ggttggagcg gtgagggatt ccgactcatg ggaggagaag ggatatcaac
    11641 ggtcgtcgcc ggacgcggcg atctccgtga tgcgcagctg ggcacgctcc agccgatcct
    11701 ggcatgtcgc gatcagcgac atcccctgct cgtagcgctg cagcatatcc tcgagagaga
    11761 ggtctccggc ttccagctcc tcgacgaggg ttttcagctc ttcaagtcgc tcttcgaagc
    11821 ttggctcttc cagtgcagat gtatcggtgg cgttcatgac tgctgcgcat cctcctgtga
    11881 atcgttggtg tgcggggcga tcgtgccgcg ggcagttcca tcccggaaat tgactaccac
    11941 tgccgtgtcg ccgtccaggt ccgccacgcg acggatcggc gtacggggtg cgggcgtttc
    12001 gatcactgca tatccgcgtt cgagaacacg ggtgggatcc agcgcggcaa ggaccttcgc
    12061 cgcgctccgg agcgacgttt cccgctgctg cagatagctc cgccccgaga gccggaggcg
    12121 gcgctccagt gtgacgagtt cctgcccagt gcgtgagatg tgcgcccgcg gcgattgctg
    12181 ctggaaacgg cgcgtgagcg cgtcgagctg ggaccattcg ttggcaagcc gccgctccgc
    12241 gatttcgatc
//
LOCUS       DASYWL010000018        15003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_507448, whole genome shotgun sequence.
ACCESSION   DASYWL010000018 DASYWL010000000
VERSION     DASYWL010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 15003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15003
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507448"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="VGR22_00680"
     CDS             complement(<1..707)
                     /locus_tag="VGR22_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127116.1"
                     /translation="MSYTPHAAPPLALGTGLRSLLAVTIPSHSLQRCPLLTLARRVMI
                     PSRASSMEDDGLVLKAGKDRPMHDDREPIGRIVRLQVQAAHLKRGEQPSRWYDPAPIT
                     EVATLRLDEGGVTGIAIDGTAHHDVHHRDHPLSRNRGDNGISVGFTGHYAAMRERFGD
                     QLMHGIAGENILVECNTVQTEPGPGGRLIIETGNGLVRLEEVIAAPPCVEFVRFCLQW
                     PRDRKPDRTVTEALQFL"
     gene            complement(816..2183)
                     /locus_tag="VGR22_00685"
     CDS             complement(816..2183)
                     /locus_tag="VGR22_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEV2127117.1"
                     /translation="MAITTINPATGETLATYDEFSTSEVNAAIEAAHQAYLGWRETSF
                     AERAERLHALAAILREDQERLATLMTCEMGKPITQARAEIEKCAWGCDFYAEHGEAFL
                     KSEVIETDARKSYVAYRPLGVVLAIMPWNFPFWQVLRYGAPALMAGNATVLKHASNVM
                     GSALAIEELFVRAGFPKGLFRTLVVRSDAVERILDDPRIAAATLTGSTAAGKAVAGAA
                     GSRVKKTVLELGGSDPYLILEDANLDAAVTACAAGRLQNSGQSCIAAKRFIVVDAVRE
                     AFEQKLVDAFRTYRMGDPLDDETNIGPLAREDLRDDVHAQVMKSVEAGAKVLIGGEIP
                     EGKGAWYPPTILDSVRQGMPVHDEEVFGPVAAVIATKDEDEAVSIANNTRYGLGAAIF
                     TEDFERGERIAAERIEVGSCFVNAFVKSDPRLPFGGIKESGYGRELSHQGIREFTNIK
                     TIYIA"
     gene            2381..3907
                     /locus_tag="VGR22_00690"
     CDS             2381..3907
                     /locus_tag="VGR22_00690"
                     /inference="COORDINATES: protein motif:HMM:NF022525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HEV2127118.1"
                     /translation="MGDATIYLTGTDVLNYRICPAWCWTAKHRPDLVPPATEHDLRRE
                     DDEARVAAVARTRFPNGALIDSEDPAEATRLTEQAIAAGAATFLSASVVSPSGLHARA
                     DILARHPSGEGWRLIEVRASTSIDAEKRLDATFQRIAFADAGYDIRSVEVMYLNKRYR
                     RNGHLDLDDLFRFDISVIRWGDGHRSRILADIDAAWDVVHGDSCPPCRCDCVTRGRRC
                     PTFDLFHPSFPNGGETVFDLVSINQKRLAEVLERGVIHLADWPEDLSLSQRQQWQVEA
                     VRSGEELVREEMLRAFLAEVEFPVHFLDYETFQVPVPLHTGMWPYQQVPFQYSVHVLH
                     EDGTLEHREFLWKEAGAPSIRPIAESLREHIADEGSVVVWWKGFEGKRNEELAAAVPE
                     LAEFFHGLNTRMIDLMETVTRGMWVHPGFNGSSSIKKVLPVVDPDMSYDELEIGHGSV
                     ATLRWKQCVVDETPPEGVDPDEAFDQLRQYCRLDTLAMVRIWEHLGRLAGIGVSALAP
                     "
     gene            3904..4932
                     /locus_tag="VGR22_00695"
     CDS             3904..4932
                     /locus_tag="VGR22_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127119.1"
                     /translation="MNGPAQLTCPVCGAPRQPGTTRCAYCGSWLVIFPGEDLPADEAA
                     VQEHVARFREVLDRDPHDVVAMHGLGVALRTLGLIDDATRILTRAANRRPESLAIQRA
                     LAGTLRDAVRNRPDDPRMWRDVRRQADRILALDPGAVDGWRLQAEVALRTRDAEALIT
                     LAPDLARHDPDGGHRAVARRLRQLGDRWFRDWRWPEAVDAWEALAALDPAAGRTALAA
                     FLVENARLVPRSEGRIWRAVRQTMALRGDFRTSTLAALVLGLAAAIALALATWWLARD
                     RFPVVTVIGLLVLPLLNTIAVRTWLAGWPPFSVPHRPWSEIGTEEMARVARMIAPKIE
                     HIRPGNEG"
     gene            5019..5564
                     /locus_tag="VGR22_00700"
     CDS             5019..5564
                     /locus_tag="VGR22_00700"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV2127120.1"
                     /translation="MTFDEGQQGSATDARDTPAGPQVVRSRNRTSLDFGSFGVEFLIW
                     GEETGGRFSVVEHPIPPRTLVAPLHRHEREDEYSIVLTGRMGARLGDDVVTSEAGDVV
                     FKPRGQWHTFWNAGDAPCRVLEIISPSGFEHLFREMGEDPEAMTGDTAETLDRRFALE
                     VDYDSVAWLCEEHGLTFPVDA"
     gene            5590..6327
                     /locus_tag="VGR22_00705"
     CDS             5590..6327
                     /locus_tag="VGR22_00705"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEV2127121.1"
                     /translation="MPTRPPSHVRARDLGIAPGRLASGPLNAITDVPGVHVFQVTVRR
                     EPDLHTGVTAVVPEQLRERRSLPAGLFVGNGFGKLVGATQLVELGEIETPIVLTGTLS
                     AFRAADALVTHMLSLPGNEHMRSVNPVVGETNDGFLPDIRSRPITDRHVLDALEGAKG
                     GPVEEGCVGAGTGTTALGFKAGMGTASRAIDIPEIRMSVSIGVLVQANFGGILRVLGV
                     PRLSIRTGRACEPRACDRGQSVRSCGA"
     gene            complement(6456..8750)
                     /gene="metE"
                     /locus_tag="VGR22_00710"
     CDS             complement(6456..8750)
                     /gene="metE"
                     /locus_tag="VGR22_00710"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEV2127122.1"
                     /translation="MAYATILGYPRIGKNREMKKALEAFWAGKISEDELRVTGEEVRR
                     AGWEAQAGAGLDLMPTNDFTFYDHVLDTTALLGNVPERYGWQGGEVDLDTYFAMARGR
                     TGERDVPAMEMTKWFDTNYHYIVPELHPGTTFKVSSRRPFEELDEAQAAGANAMPVLL
                     GPVSYLLLGKSHDEHFDRLALIERLAPVYAEIVDRLAKSGAEWIQIDEPVLVQDLVPG
                     EMEALKTAYETIAAAKGTAKIVVQTYFDHVGEAYEVLVNLPVDGIGLDLVRGRKNLDL
                     IERHGFPEGKVLVAGIVDGRNVWVNDLNASMDTLERLAEMIPGDNLMVSTSCSLLHVP
                     YTVENERDLDPEVKRWLAFAEEKVNEVVLLSAGLTRDRDAIADELRANTALLREASES
                     PRRRNPDMQERLANLPPDVTDRGRPFAERAQIQRERLGLPKLPTTTIGSFPQTGELRK
                     ARRQFERGNLSAEEYEAFIKAQIRQVIEHQESLGIDVLVHGEPERNDMVQYFGEQLEG
                     FAFTRNGWVQSYGSRYVRPPIIFGDVSRPEPMTVKWAEYAQSLSDKPVKGMLTGPVTI
                     LNWSFVRDDQPRAETCRQIALAMRDEVADLDAAGIRMIQIDEAALREGLPLRKEDWQA
                     YLEWAVECFRITAAGARPETQVHTHMCYSEFNDIFGAISDLDADVISIENARSGLELL
                     EAFREQGYDKGIGPGVYDIHSPRVPPAEEIAENLKATLTVLDVDQVWVNPDCGLKTRK
                     PEEATPALQNMVAAARQVRESLAG"
     gene            complement(9024..9992)
                     /locus_tag="VGR22_00715"
     CDS             complement(9024..9992)
                     /locus_tag="VGR22_00715"
                     /EC_number="4.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threo-3-hydroxy-L-aspartate ammonia-lyase"
                     /protein_id="HEV2127123.1"
                     /translation="MTTELALPTYTDIESAVQRIEGIVHRTPVLTSRHANETFGAAFF
                     FKAEHLQRMGAFKFRGAVNALSRFSPEQRRAGVVAFSSGNHAQAIALAARELGIPATI
                     VMPEDAPQMKIAATRGYGGETVLYDRYTEDREAIGRQIAESRGMTLIPPFDHPDIIAG
                     QGTAAKELIEEVGQLDALLVPLGGGGLLSGSVLAAKALSPGIEIYGVEPEAGNDGQRS
                     FRSGEIVHIETPKTIADGAQTLHLGQITFPIIKRDVTDILTATDEQLVEAMRFFASRM
                     KQIVEPTGTLGLAGFATIAELLSGKRVGVIISGGNVDLARFCALTS"
     gene            10181..10750
                     /locus_tag="VGR22_00720"
     CDS             10181..10750
                     /locus_tag="VGR22_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127124.1"
                     /translation="MSSKLWDYLLELDPDVALLQDFGAIPDHVLHVYTHARNTKPIVA
                     DRTPQFMTGILVKGVSDDIALPAPTEWVARELENFEEFLTAKAVTLHDRVRLRTLSVD
                     SPAFPVDRARLEGIDTTGIQLTQNRDVWGTELMWATLKSMGIGPEEPLIVGGDLNSSE
                     TFDYLWRGGPRGNREIMDRMNALECSRFL"
     gene            complement(11376..11639)
                     /locus_tag="VGR22_00725"
     CDS             complement(11376..11639)
                     /locus_tag="VGR22_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127125.1"
                     /translation="MFALFPLPPTDHQMQQRRLRYEYGEDAMAARQRQRTRQPTDPRP
                     ATWVRLAMAAMLIRVGERLLSTQAGNPAVPGLETPVDPGTAPP"
     gene            11866..14598
                     /locus_tag="VGR22_00730"
     CDS             11866..14598
                     /locus_tag="VGR22_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2127126.1"
                     /translation="MDARKPSAFGSLLRDYRVAAGLSQERLAERAGLSLRGISDLERG
                     ARTAPRLETVRMLADGLDLDGPERAALLAARGAAADAASPAPVTDRPVLPMPLTSLVG
                     REREIEAVRGLLHREDVRLVTLTGPGGVGKTRLALQLVAESPERFADGVWFVPLAPIR
                     DPDLVLLTVARALGLRDTGDRPLGERLATFLHEKAALLVLDNFEQVLAAGPSVTDLLM
                     ACPRLKVLVTSRAALRVTGEHSFPVPPLALPDLGPNGSPSFSEIARSDAVRLFAERAQ
                     AVDPGFELTEASAPVITEICQRLDGLPLAIELAAARSNTLPPVALLGRLEQRLPLLTG
                     GARDQPARLRTMRDAIAWSYDLLLPDEQAVFRRLAVFASGCMFEAAEAVVNVDSSLGL
                     DVLDGISSLVDKSLLDRVEGPGGEPRFAMLETVREFGLEQLVASEEADGARQRHAAHY
                     LELAEEAAPRLRGREQMAWLDRLEAEHTNLRAAIQWSIERGEAEVALRLAGVLHWFWY
                     LRGHWGEGRRWLERALALAPRGIVMAARTKTLAGAGVLAFAQSDYPAARVRLQESLAG
                     CQEREDVAGSAYALHFLGMTALLPGENASARALFEESVATFRQAGDRWGVATSLCSLG
                     VAAMQSEVDESRAFLEESLALYRELGDTYGVARALQYLGEVARAGGDYVVARRLYEEG
                     LTLYRALGHRNTAASVVHNLGYVAQHEGNLRLGAACFAEALAVHVEHGDRRNIGHCLG
                     GLAGMAGLLGYPEQAARLFGAADTVLAKAGASMFFVDRLDYDRNQAAVRALLGEQAFE
                     AAFLAGKALVLDQALAEAARVATVVAAIPPQPAKASITAAPSSQSAADWHGLTARERD
                     VLRLLVDGRTDREIAETLCISPRTVGVHVTNVLGKLAVGTRTAAAAHAVRHGLA"
BASE COUNT         2402 a         4772 c         5110 g         2719 t
ORIGIN      
        1 tcgaggaact gcaacgcttc ggtgaccgtc cggtccggct tgcgatcgcg cggccactgc
       61 aggcagaacc gcacaaattc gacgcaaggt ggcgcggcga tcacctcctc cagccggacg
      121 aggccgttcc cggtttcgat gatgagtctt cccccgggcc ctggctcggt ctggacggtg
      181 ttgcactcga caaggatgtt ctcgccagcg atgccatgca tcagctgatc cccgaagcgc
      241 tcgcgcatgg ccgcatagtg tcccgtgaaa ccgaccgaga tcccgttgtc gccgcgattg
      301 cgggagagcg gatggtcacg atggtggacg tcgtggtggg ccgtgccgtc gatggcgatg
      361 ccggtgaccc ctccctcgtc gagccgaagc gtcgcgacct cggtgatggg tgcggggtcg
      421 taccagcgac tgggctgctc gccgcgcttg aggtgagcgg cctggacctg gagccgcacg
      481 atccggccaa tcggttcacg gtcgtcgtgc atcggtcggt cctttcctgc cttcagaacg
      541 aggccgtcat cttccatgct actggctcgc gaggggatca tcacgcggcg cgcgagtgtc
      601 aggagggggc agcgctgcag agagtgcgac gggatcgtca cggccagcag cgagcgcaga
      661 ccggtgccga gggcgagggg aggcgcagcg tgcggcgtgt aggacatgtg gtggcgccgg
      721 agcaagagtg accaccatcg ccggctgcgt cagacgacga ggggtggcag tcaatgcccg
      781 cccctgcgct tcgggtgcaa gctgagatga gagactcagg cgatgtagat cgtcttgatg
      841 ttggtgaact cgcggatgcc ctggtgcgag agctcgcggc cgtagccgga ctccttgatg
      901 ccgccgaagg gcaggcgcgg gtcggacttg acgaaggcgt tgacgaagca gctgccgact
      961 tcgatgcgtt cggcggcgat gcgctcgcct cgctcgaagt cttctgtgaa gatcgctgcg
     1021 cccagaccgt agcgggtgtt gttggcgatg ctgaccgcct cgtcttcgtc cttcgtggcg
     1081 atgactgcag cgacgggccc gaagacctcc tcgtcatgca ccggcatgcc ctgtcggaca
     1141 ctgtcgagga tcgtcggcgg gtaccatgcg cccttgccct ccgggatctc gccaccgatg
     1201 agcaccttcg ctcccgcctc gacactcttc atcacctgtg catggacgtc gtcgcgcagg
     1261 tcctctcggg cgagcgggcc gatgttcgtc tcgtcgtcca acggatcacc catccggtag
     1321 gtgcggaagg cgtcgaccaa cttctgctcg aacgcctcgc gcacggcgtc gaccacgatg
     1381 aagcgcttgg cggcgatgca gctctggccg ctgttctgca gccgcccggc cgcgcaggcg
     1441 gtcacggcgg cgtcgaggtt ggcatcctcc aggatgaggt agggatccga gccgcccagc
     1501 tcaagcaccg tcttcttgac ccgcgacccg gccgccccgg cgaccgcctt gcccgccgcg
     1561 gtgctgcccg tcagcgtggc agcggcgatg cgcggatcgt cgaggatccg ctcgacggca
     1621 tcggagcgaa cgaccagcgt tcggaagagg cccttcggga atccggcacg gacgaagagc
     1681 tcctcgatcg ccagtgcgct gcccatcaca ttgctggcat gcttgagcac cgtggcgttg
     1741 cccgccatca gcgcgggcgc accgtagcgg agtacctgcc agaacgggaa gttccacggc
     1801 atgatcgcca gcaccacacc gagcgggcgg taggcgacat agctcttccg tgcatcggtc
     1861 tcgatcacct cggatttcag gaatgcctcg ccatgctcgg cgtagaagtc acagccccac
     1921 gcgcacttct cgatctcggc acgggcctgc gtgatcggct tgcccatctc gcaggtcata
     1981 agcgtcgcga gccgctcctg atcctcccgg aggatcgccg ccagcgcgtg gagccgctcg
     2041 gcacgctcgg cgaagcttgt ctcgcgccag ccgaggtacg cctggtgggc ggcctcgata
     2101 gcggcattga cctctgacgt ggagaactcg tcgtaggtgg cgagggtctc tccggtggcg
     2161 gggttgatcg tcgtgatggc catgtggggc tcctttctcg gtcgggctgt caactccaag
     2221 ggtacggcat cggggcgggg ttcgtgcgca caacacaata tgttatgaca ggaatcggcg
     2281 atactggacg acggcagtgg agttgtgccc tgctatactt gtagtggatg taaaatgtgt
     2341 acgtacgtta cgatcgaacc ggggagcacg cgagcgacgg atgggtgacg cgacgatcta
     2401 tctgacaggg accgatgtcc tcaactaccg catctgccct gcttggtgct ggacagcgaa
     2461 gcaccgcccc gatctcgttc cgcccgcaac cgaacacgac ctgcgccgcg aggacgacga
     2521 ggcgcgcgtc gccgccgttg cccgcacacg cttccccaat ggcgccctga tcgacagcga
     2581 agacccggcc gaggcgaccc gcctgaccga gcaggcgatc gcggcggggg cggcaacgtt
     2641 cctctcggcc tcggtggtgt cgccgtctgg cctccacgcg agggcagaca tcctcgcgcg
     2701 gcatccctcc ggcgaggggt ggcggctcat cgaggtccga gcttcgacct cgatcgatgc
     2761 cgagaagcgg ctcgacgcga ctttccagcg catcgccttc gctgacgcgg ggtacgacat
     2821 ccgcagcgtc gaggtgatgt atctcaacaa gcggtatcgc cgcaatggcc acctcgatct
     2881 cgacgatctc ttccgcttcg acatttctgt catccgctgg ggagacgggc accggtcacg
     2941 gatactcgcc gacatcgacg ctgcctggga tgtggtgcac ggcgactcct gcccgccctg
     3001 tcggtgcgac tgtgtcacgc gcggtcgtcg ctgcccgacc ttcgatctct tccacccgtc
     3061 cttcccgaat ggcggcgaga cagtgttcga tctggtctcc atcaaccaga agcggctcgc
     3121 ggaggtgctg gagcgcgggg tgatccacct ggccgactgg ccggaggacc tctcgctgag
     3181 ccagcgccag cagtggcagg tcgaggccgt ccgctccggt gaggagttgg tgcgcgagga
     3241 gatgcttcgc gcgtttctcg ccgaggtcga gttcccggtc catttcctcg actacgagac
     3301 cttccaggtg ccggtgccgc tgcataccgg catgtggccc taccagcagg tgcccttcca
     3361 gtattccgtc cacgtgttgc acgaggacgg cacgctggag caccgcgagt tcctctggaa
     3421 ggaggccggc gctccatcga tccgtcccat cgccgagtcg ttgcgcgagc acatcgccga
     3481 cgagggttcg gtggtcgttt ggtggaaggg cttcgagggc aagcgcaacg aggagctcgc
     3541 tgccgccgtg cccgagctgg ccgagttctt ccacggactc aacacgcgga tgatcgacct
     3601 catggagacg gtcacgcggg gcatgtgggt gcatcctggt ttcaacggca gctcctcgat
     3661 caagaaggtg ctgccggtgg ttgacccgga catgagctac gacgagctgg agatcgggca
     3721 cggctcggtt gcgacgctgc gctggaagca gtgcgtggtc gacgagacgc cgccagaggg
     3781 agtcgatccg gacgaggcgt tcgaccaact tcgccagtac tgccggctcg atacgctggc
     3841 aatggttcgc atctgggagc atctcggtcg tctcgccggg atcggtgttt cggccctagc
     3901 gccgtgaacg gaccggcgca actgacctgc cccgtctgtg gcgcgccccg gcaacccggc
     3961 accacccgct gcgcctactg cggcagctgg ctggtgatct tccccggcga ggatctgcca
     4021 gccgacgagg ccgcggttca ggagcacgtc gctcgtttcc gagaggtgct cgaccgcgac
     4081 cctcacgatg tcgtcgcgat gcacgggctc ggcgttgcgc tccgcaccct cggcctgatc
     4141 gacgacgcca cgcgcatcct cacccgagca gccaaccgac ggccggagtc gctcgcgatc
     4201 cagcgggcgc tcgctggcac gctccgcgac gccgtgcgaa accggccgga cgacccgcgc
     4261 atgtggcgag acgtgcgccg gcaggccgat cgcatcctcg cgctcgatcc gggggccgtc
     4321 gatggttggc ggctgcaggc cgaggtcgcc ctgcgcaccc gcgacgccga ggcgctgatc
     4381 acgctggcgc ccgacctcgc ccggcatgat cctgacggcg gccaccgggc tgtggccagg
     4441 cgcctgaggc agctggggga tcgttggttt cgggactggc gctggccgga ggcggtcgat
     4501 gcctgggagg cgctcgctgc gctcgacccg gctgccggcc gaaccgcact ggcggctttt
     4561 ctggtggaaa acgcgcggct ggtgccgcgt tccgaaggac ggatctggcg ggcagtgcgg
     4621 cagacgatgg cactgcgcgg cgatttccgg acttcgacgc tcgctgcgct ggtcctcggg
     4681 cttgcggcgg cgatcgcgct ggcactggcc acctggtggc tggctcggga tcgctttccg
     4741 gtcgtcacgg tgatcgggct gctggtgctg cccttgctca acacgattgc cgtgcggacc
     4801 tggctggcag gttggccacc cttctcggta ccgcaccggc catggtcaga gatcggcacg
     4861 gaggagatgg cgcgggtcgc ccggatgatc gcgccgaaga tcgagcatat ccgcccagga
     4921 aacgaggggt agcgggccgg aggctttgac agcccttcgg cgctctctat actgaccaaa
     4981 cgagtcctcg gtcttcgtca tcgtgaggga ggtgtgggat gacgttcgat gaagggcagc
     5041 aggggagcgc aactgatgcc cgtgatacgc ctgctggtcc tcaggtcgtc cgaagccgga
     5101 atcggacgag cctcgatttc ggatccttcg gtgtcgagtt cctgatttgg ggcgaggaga
     5161 ccggaggcag gttctccgtg gtcgagcacc cgatccctcc gcggacactc gttgcacccc
     5221 tgcaccggca tgagcgcgag gacgagtaca gcatcgtcct cacagggcgg atgggtgccc
     5281 ggctcggcga cgatgtggtg acctccgagg caggtgatgt ggtgttcaag cccaggggac
     5341 aatggcacac cttctggaac gccggcgacg cgccgtgtcg agtgctggag atcatctctc
     5401 caagtgggtt cgagcatctc ttccgcgaga tgggcgagga cccggaggcg atgaccggtg
     5461 acacggctga gacgctcgac cgccggtttg cccttgaggt cgattacgac agcgtggctt
     5521 ggctgtgcga ggagcacggc ctcaccttcc cggtggatgc ctgaccaccg ttcacgaacg
     5581 gagaacgccg tgccgacgcg cccgccgtca catgttcgcg cccgagacct cggtatcgct
     5641 ccggggcggc tcgcgtctgg gccgctcaac gcgatcaccg acgtgcccgg cgtgcatgtg
     5701 ttccaggtga ccgtccgacg tgagcccgat ctccacaccg gcgtcactgc ggtagtgccg
     5761 gagcagttac gggagcgcag gtcgctgccg gccggactct tcgtcggcaa tggcttcggc
     5821 aaactggtgg gcgcgacgca gctcgtcgag ctgggtgaaa tcgagacgcc gatcgtgctg
     5881 accggcacgc tctcggcctt ccgggcggcc gacgcgctgg tgacccacat gctgtcactg
     5941 ccgggaaatg agcacatgcg atcagtcaac cctgttgttg gagaaaccaa cgacggcttc
     6001 ctccccgaca tccggtcgag gccgatcacc gaccggcacg tgctcgacgc gcttgagggc
     6061 gcgaagggtg gcccggttga ggagggatgt gtcggcgccg ggaccggcac gacggcgctc
     6121 ggtttcaagg caggcatggg aacggcgtcg cgcgccatcg atattccgga gatacggatg
     6181 agtgtatcaa tcggcgtgct ggtgcaggcc aacttcggtg gcatcctgcg tgtgctgggc
     6241 gtaccgaggc tgtcgatccg aacaggtcga gcctgcgaac cccgtgcttg cgacagaggg
     6301 cagtcagttc gctcatgtgg tgcttgatga gtggaatcac ggctccaaac cctctgattg
     6361 gaaggattcc cgagttggct gtctaccgac tgcgggccgg cacttaggcc gacccctacg
     6421 agcctacagg aacccaagca cctcagctct ttgggtcagc ctgcgagcga ttcgcggacc
     6481 tgcctcgcgg cggcgaccat gttctgcagt gccggggtgg cctcctcggg cttgcgcgtc
     6541 ttcaggccgc agtccgggtt cacccacacc tggtcgacat cgagcaccgt cagcgtcgcc
     6601 ttcaggttct cggcgatctc ctcggccggc ggcacgcgcg ggctgtggat gtcatagacg
     6661 cccggcccga tgcccttgtc gtagccctgc tcgcggaatg cctccagcag ttccaggccg
     6721 gaccgcgcgt tctcgatcga gatcacgtcg gcgtccagat cgctgatcgc cccaaagatg
     6781 tcgttgaact ccgagtagca catgtgcgta tgcacctgtg tttcagggcg agcgccggcg
     6841 gcggtgatgc ggaagcactc gaccgcccac tcgagatatg cctgccagtc ctccttgcgc
     6901 aggggcagcc cctcgcgcag cgccgcctcg tcgatctgga tcatgcggat acccgcggca
     6961 tcgagatctg ccacctcgtc ccgcatcgcc agcgcaatct gccggcaggt ctccgcacgt
     7021 ggctggtcgt cccgcacaaa tgaccagttg aggatcgtga ccgggccggt aagcatgccc
     7081 ttgaccggct tgtcgctgag cgactgcgcg tactcggccc acttgacggt catcggctct
     7141 gggcgggaga catcgccgaa gatgatcggt gggcgcacgt agcgcgagcc gtaactctgc
     7201 acccagccgt tgcgggtgaa ggcgaatccc tccagctgct cgccgaagta ctgcaccatg
     7261 tcgttgcgct ccggctcgcc atggaccagc acgtcgatgc cgagcgactc ctggtgctcg
     7321 atcacctggc ggatctgggc cttgatgaac gcctcgtact cctcggcgga gaggttgccc
     7381 cgctcgaact ggcggcgcgc cttgcgcagc tcgcccgtct gtgggaagga gccgatcgtc
     7441 gtggtcggca gtttcggcaa tccgagccgc tcacgctgga tctgcgcgcg ctcggcgaac
     7501 ggccggccac ggtcggtgac gtcaggtggg aggttggcga ggcgctcctg catgtctgga
     7561 ttgcgtcgcc gcggtgattc cgacgcctca cgcagcagcg cggtgttggc ccgcagctca
     7621 tcggcgatcg catcgcgatc gcgggtgagt cctgccgaga gcagcaccac ctcgttcacc
     7681 ttctcctcgg cgaaggcaag ccagcgcttc acctcggggt cgagatcccg ctcgttctcg
     7741 acggtgtagg gcacatgcag cagcgagcag gaggtggaca ccatcaggtt gtccccggga
     7801 atcatctcgg cgagccgctc gagcgtgtcc atcgaggcgt tgaggtcgtt gacccagacg
     7861 ttgcggccat cgacgatgcc tgcgaccagc accttgccct ccgggaagcc gtgccgctcg
     7921 atgaggtcga ggttcttccg gccgcgcacg agatcgagcc cgatgccatc gaccggcagg
     7981 ttgaccagca cctcgtacgc ttcgccgacg tggtcgaagt aggtctggac aacgatcttc
     8041 gccgtgccct tcgccgcggc gatcgtctcg taggccgtct tcagcgcctc catctcgcca
     8101 gggacgaggt cctgcacgag gaccggctcg tcgatctgga tccactcggc gccgctcttc
     8161 gcgagccggt cgacgatctc ggcatagacc ggcgcgagcc gctcgatcag cgcgaggcgg
     8221 tcgaagtgct cgtcgtgcga cttgccgagc agcagatagc tgactggtcc gagcagcacc
     8281 ggcatggcgt ttgcgcccgc cgcctgcgcc tcatcgagtt cctcaaacgg gcgcctgctg
     8341 gaaaccttga aggtggtacc cgggtgcagc tccgggacga tgtagtggta gttggtgtcg
     8401 aaccacttgg tcatctccat cgcgggcacg tcgcgctcgc cggtgcggcc gcgcgccatc
     8461 gcaaagtagg tgtccagatc gacctcgccg ccctgccagc cgtagcgctc cggtacgttg
     8521 ccgagcagtg cagtggtgtc gagcacgtgg tcgtagaagg tgaaatcgtt ggtcggcatc
     8581 aggtcgagtc ccgcgcccgc ctgggcctcc cagccggcgc ggcgaacctc ttcaccggtc
     8641 acgcgcagct cgtcctcgct gattttgccg gcccagaacg cctccagcgc cttcttcatc
     8701 tcgcggttct tgccgatgcg ggggtatccg aggattgtgg cgtaggccat gtgcgcctcc
     8761 atctcttctg tgcggggtcc gataggcgtg acagtgacgg cgatgccgtt gcctggcgcc
     8821 atgcggatgc tgcgaatctg cccaagtata gctatgcagt cattgactat gacctcgccg
     8881 gtgctcagct cccctgagtg gcctccggac gaacgcggcc cgatcgtgtc catgattgca
     8941 atgcaggtca acgatgcgga gtcaccgctc ccccgtggaa gcggtgaaga caggtggtgt
     9001 gctggggaga ctcgtccttc ggatcagctg gttagcgcgc aaaagcgcgc gaggtcgacg
     9061 ttgccaccgg agatgatgac gccgacacgc ttgccggaga gtagctcggc gatggttgcg
     9121 aacccggcga ggccgagcgt gccggtcggc tcgacgatct gcttcatgcg gctagcgaag
     9181 aagcgcatcg cctccaccag ctgctcatcg gttgcggtga ggatgtcggt gacatcacgc
     9241 ttgatgatcg ggaaggtgat ctggccgagg tggagcgtct gcgcaccgtc ggcgatcgtc
     9301 ttcggcgtct cgatgtggac gatctcgccg ctccggaacg agcgctgccc gtcgttgccg
     9361 gcctccggct caaccccata gatctcgatg ccggggctga gcgctttcgc cgccagtacc
     9421 gaacccgaga gtagccctcc gccgccgagc ggcaccagca gcgcgtccag ctggccgacc
     9481 tcctcgatca gctccttcgc agcggtgccc tggccggcga tgatgtccgg gtggtcaaag
     9541 ggagggatca gcgtcatgcc gcgcgactct gcgatctgcc ggccgatcgc ctcgcgatcc
     9601 tcggtatagc ggtcgtaaag cacggtctcg ccgccatagc cccgcgtggc agcgatcttc
     9661 atctgaggag cgtcttccgg catgacgatc gtcgccggga tgccgagctc gcgggcggcg
     9721 agcgcgattg cctgggcgtg gttgcccgag gagaaggcga ccaccccggc gcgccgctgc
     9781 tctggcgaga agcgggagag ggcattgacg gcaccacgga acttgaaagc gcccatccgc
     9841 tgcagatgct cggccttgaa gaagaacgcc gcgccgaagg tctcgtttgc atggcgggac
     9901 gtgagcaccg gcgtccgatg cacgatcccc tcaatgcgct ggacggcgct ttcgatatcg
     9961 gtataggtgg gcagggcaag ttcggtagtc aatgtgatgg ctctctggtc agcacaattg
    10021 gaaagcagtc gtaggggcgg acctcgcaca cattgccacg gaacaccgac cggcacaaga
    10081 aggctgttcg cactcctggt ctgctgcgcg cacttgcctg ctatcatcct gaatcacgaa
    10141 gcagacattc cgcgtcgtta cttggaattg caggcgagct ttgtcgagca agctctggga
    10201 ctatctcctg gagctcgatc cggacgtggc gctactccaa gacttcgggg ccatccctga
    10261 tcatgtcctg catgtataca cccacgcccg aaacaccaaa cccatcgtgg cggatcgcac
    10321 gccacagttc atgaccggga tccttgtgaa gggcgttagc gatgatattg ccttgcccgc
    10381 tccgacggag tgggtggctc gggagttgga gaatttcgaa gaattcctca ctgcaaaggc
    10441 agtaacgctt cacgaccgcg tccgcctcag gacgctatcc gtcgatagcc ctgcgtttcc
    10501 cgttgaccgg gcacgactcg aaggtatcga cacaaccggc attcagctga cccagaaccg
    10561 cgatgtctgg ggtactgagt tgatgtgggc gacattgaag tcgatgggga tcggtccaga
    10621 agagccactc atcgtgggcg gcgatttgaa ctcctcggag accttcgact atctctggcg
    10681 gggtggaccg agaggcaacc gggaaatcat ggatcgaatg aatgctcttg aatgctctcg
    10741 gtttctatga ttgctcgcga acgttcaagg gagagttgac tccaaccttt cgtacaccga
    10801 gaggtgggac gatcgttcat cagctcgatc atctttacgt gaccccagtg ctcttgggca
    10861 ggcttgcttg gtgcgatgtc ggttcggctg aacgtgtctt cgggtccaag ccgacgctga
    10921 gcggtcacct gccaatagtg gctgattttg tttggtcgag tgacgcaaca ccggacagca
    10981 cggaatgagc ggctctgttg ccaggaagcc gtgtacgttg cactgctgga gaaggacgga
    11041 gccatggccg ctctcggaac gcccgtcaca gccaggagac gacgggttcc actaatacgc
    11101 ctcgagggga ggctaggagt ggcctagcac cggttatgtg ggtgctggtc acaaacacta
    11161 cggctggtta gctaggacgc atccgaaatc gcagcgatgg tgatctgagt gatgctgtct
    11221 acactccact gctcctatat caggaagcat tagtgagaca ggtttcccgg taaggcggca
    11281 tccgtggcac cggacgctcc ggcaacgtag cgccgttggg caagcaggag ggggcatcag
    11341 cacagccgcc cctgcttgca ccgtttggcc cggtgctatg gcggtgcagt gcccggatcg
    11401 acaggtgtct cgaggccagg aacggccggg ttgccggcct gcgtgctcag gaggcgctcg
    11461 ccgacccgaa tcagcatcgc cgccatggcc aggcggaccc aggtggcggg gcggggatcg
    11521 gtcggctggc gcgttcgctg ccgttgccgg gcagccatcg catcttcgcc gtattcatag
    11581 cggaggcgac gctgctgcat ttggtggtcg gtcggaggga gtgggaacag ggcgaacatg
    11641 tcatcctcct tgggatagcg acgttgacag tggggacggc cccggcttct ccgggaacag
    11701 ttccatcgtg cgccaagctg gtgccgaggc ctacgtgcat cgaccacgcg ttgcccacgc
    11761 aacggaccgc tttggcacgg gaggctgcgg tgacatcccg cccggccggt gctaaggtgg
    11821 ggatggggaa gtgcacaagg tggctgcagg agaggtggga gcgccatgga cgcacggaag
    11881 ccttcagcgt tcgggagcct gttgcgggac taccgtgtgg cggcgggctt gtcgcaggag
    11941 cggcttgccg agcgcgctgg actgagcctg cgcgggatca gtgacctgga gcgcggtgcc
    12001 cgcacggctc cccggttgga aaccgtgcgg atgctggctg acggtctcga cctggacgga
    12061 cccgagcggg ccgctctcct tgccgcccgc ggtgcggccg cggacgcggc ctctcccgca
    12121 cctgtgaccg accggccggt gctcccgatg ccactcacct cgctggtcgg ccgggagcgt
    12181 gagatcgagg cggtgcgtgg cttgctgcac cgggaggacg tgcggctcgt cacgctcacc
    12241 ggtcccggtg gtgtgggcaa gacccggctc gccctccagt tggtagccga atcgccggag
    12301 cgctttgccg acggtgtctg gttcgtgccg ctggcgccga tccgcgaccc ggatctggtc
    12361 ctgctcactg tcgcgcgggc actcggtctc cgggataccg gtgaccgacc gctgggcgag
    12421 cgcctggcca ccttcctgca cgagaaggcg gcactgttgg tcctcgacaa cttcgagcag
    12481 gtgctggcgg ctggcccgtc ggtcactgac ctgctcatgg cgtgccctcg cctgaaggtg
    12541 ctggtcacca gtcgggcggc gctgcgcgtc accggcgagc actccttccc cgtgccgccc
    12601 ctggccctgc ccgaccttgg accgaatggc agcccgtcct tcagcgagat agcgcggtct
    12661 gatgcggttc gcttgttcgc tgagcgggca caggccgtgg atcccggctt cgagctcact
    12721 gaggctagcg cgccggtcat caccgagatc tgtcagcggc tggatgggtt gccgctcgcc
    12781 atcgagctgg ctgctgctcg cagcaacacc ctgcccccgg tcgccttgtt ggggcgcctg
    12841 gagcagcggt tgccgctcct gacgggtggt gcgcgggacc agccagcgcg gctgcggacc
    12901 atgcgcgatg ccatcgcctg gtcctacgat ttactgctgc cagacgagca ggcggtcttc
    12961 cgacgcctag ctgtctttgc cagcggctgc atgttcgagg ccgcagaggc ggtggtcaac
    13021 gtcgactcta gccttgggct cgatgtcttg gacggcattt cgtcgctcgt ggacaagagc
    13081 ctcctagatc gggtcgaggg accaggcgga gagccacgct tcgcaatgct cgagacggtg
    13141 cgtgagttcg ggctcgagca gttagtggct agcgaggaag cggacggtgc acggcaacgc
    13201 cacgcggcgc attacctgga gctggccgaa gaggcagcgc cgcggctccg agggcgtgag
    13261 cagatggcat ggctggaccg actggaggcc gagcacacca acctccgggc ggccatccag
    13321 tggtcgattg agcggggtga ggctgaggtg gcactacggc tggcgggcgt cctgcattgg
    13381 ttttggtatc tccgtggcca ctggggcgag gggcgccgct ggctggagag agcgctggca
    13441 ctggctcccc gtggaatcgt gatggcggcg cggacaaaga cactggccgg ggccggggtc
    13501 cttgcctttg cccagagcga ctacccggcc gcgcgtgtgc ggctccaaga gagtctcgcc
    13561 gggtgccagg agcgggagga cgtggcgggc agtgcctacg ctctgcactt cctcgggatg
    13621 acagcgctcc tgcccggtga gaatgcgtcg gcccgtgcgc tctttgagga gagcgtcgcc
    13681 acctttcgtc aggccgggga ccggtggggg gtggcgacat cgctctgttc gttaggggtc
    13741 gcggcgatgc agtcggaggt cgacgagtct cgcgccttcc tagaagagag cctcgccctc
    13801 taccgcgaac tgggggacac gtacggcgtg gctcgagcgc tgcagtacct cggcgaggtg
    13861 gcacgcgcgg ggggcgacta cgtcgtggcg aggcggctct acgaggaggg cttgaccctc
    13921 tatcgggcgc tgggtcaccg gaatacggcc gcgagcgtcg tccacaatct cggctatgtt
    13981 gctcagcacg aaggcaacct gcggctggga gcggcctgtt tcgccgaggc gctggcggtg
    14041 cacgtcgagc acggtgaccg ccgaaacatt ggccactgcc tgggtgggct agcagggatg
    14101 gccggcctgc ttggctatcc cgagcaggcg gcacggctgt tcggcgcggc agacaccgtg
    14161 ctcgccaagg ccggtgcctc catgttcttc gtcgatcgcc tcgactatga ccgcaatcaa
    14221 gcggcggtcc gggctctact tggcgagcaa gccttcgagg ctgccttcct agccggtaag
    14281 gcactggtgc tggaccaagc acttgccgag gcggcgcgcg tggcaactgt cgtggcagct
    14341 atacctccac aaccagccaa ggccagcatc acagccgcgc catcctctca gtcagcggct
    14401 gattggcatg gtctgacagc ccgtgagcgc gatgtcctac gcctgcttgt cgacggccgc
    14461 accgaccggg agatcgcgga gaccctctgc atcagccctc gcaccgtggg cgtccacgtc
    14521 actaacgttc tcggcaagct ggccgtcggg acgcgtactg ccgccgccgc ccacgccgtt
    14581 cgccacggtc ttgcctgacc cacagcccca cgacctacgc agatctacct atatcgtgcg
    14641 cgagagcggc gggatcaaat acggggtctt cacgatgccc tctaggccac aagaccgcat
    14701 gatgaaatca tgctgcttga gataagcggc atgccgagac gaccaggcgg cggccgctcg
    14761 gcgccgccga aggcgacagc ataacggtgg aggatagctg cggtcctgat tgccaggagc
    14821 ggttgggact cgatggcatg gaggtatcca atgattacgt cgaagatcgt gagtgaggcg
    14881 aatgcggcgg agctcgccgg gtcggtcgtg gcgctgtggc actaccccgt caagtccatg
    14941 cagggcgaag agctcaccgc actggccttc gggccgcggg gggccgtcgg ggaccgggcc
    15001 tat
//
LOCUS       DASYWL010000019         4055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_282325, whole genome shotgun sequence.
ACCESSION   DASYWL010000019 DASYWL010000000
VERSION     DASYWL010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282325"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..2793)
                     /locus_tag="VGR22_00735"
     CDS             complement(145..2793)
                     /locus_tag="VGR22_00735"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2127127.1"
                     /translation="MPDLPHGNVTFLFTDLEGSTRLWRRYAEAMPDAYARHDLLLHRA
                     VEAHRGVIYKVVGDGCQAAFASAGDALDAALEAQRELVAEEWQTPEPLRSRMALHTGA
                     AEPQPNGDYRTAILNVPGRLLGAGYGGQVLLSAATAELVRDLLPSGVALRDLGERRLR
                     DLPPARIYQLLAPGLPATFPPLNTIDARSHNLPEPPTPLVGREREVAAATAQLRSGSV
                     RLLTLTGPGGTGKTRLALAIAADLIDDFDHGVTFVALAPVTDPALVASTIARELGVQE
                     EAGRDLADLLIAHLREKRLLLVLDNFEQVAPAASLIAQLLAACPGLTVLATSRMRLHL
                     RAERGFPVPPLALPDLAAPIDTLMQAEAARFFVDRARAISPDFIVNVAVARSIAEVCH
                     RLDGLPLAIELAAAWVTDLTPGEMLARLETRLPLLTDGAFDLPDRQRTLRSTIDWSYD
                     LLSRDEQTLFRRLAVFAGGWLREAAVAVGNPPDHTGIDVDGGLTSLVDKSLIRIDTDA
                     RGDRYTMLETIREYGLDQLLASGEAEAVRRAHATQFLTFATNAAPGTWGSDQADWLDR
                     LDLELDNLRAALTWAIDRAEAQTALDLGISMEKLWDVRGSLDEGRRWLEQALALPMDG
                     VRPAARAQAHSNAGSIAQSQGDLEGARRLQEQALAILVDLDTEGGQRGTAHTLNRLGI
                     VAFLQGDSERADTLHEEALERFRDLDDKSAMATVLNNLGVTADHREEYDRARSLYEEA
                     LALQREAGDTQSIAIYLGNLGEVTRDQGDFPQAAAYYREALLLWAELRDRWNLTATLD
                     GVAELAIAYKQPHHAARLLGAGAALRAAAGVPLPENERAAYERSVAAAREALGDGAFD
                     VEWAAGQAMTYDEAIREASAVIRAME"
     gene            2969..3196
                     /locus_tag="VGR22_00740"
     CDS             2969..3196
                     /locus_tag="VGR22_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127128.1"
                     /translation="MATDQLSRLVERAMNDPAFISRAQADLDGTLAAEDISLDSDEMA
                     AVREFHSQIVGLSEEEIQGRLSESNRQQGPM"
     gene            3273..>4055
                     /locus_tag="VGR22_00745"
     CDS             3273..>4055
                     /locus_tag="VGR22_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV2127129.1"
                     /translation="MSVTPEQVELLSLVVDEGRSQAASLPNGLPSAEVTMAAYAAAGG
                     GDDLPLPVTAACLCVYLGADVLDNVIDRELSDRWEEHGAHQALLAGVTVLTPLSIACL
                     GELNGPIERRLAAQRILIDTLITMAAGEVADVAFEGRDDVSLDACEAMVLAKSGAECS
                     LFAQVGAIVAGAPSDVCDAYATFGRELGAAIQLVSDCADLATGDEGRDLVSGKRTLPI
                     VHALTVLPDAKRAELRDHLAASDDDERRRAARQMLLDAGSFHF"
BASE COUNT          666 a         1326 c         1365 g          698 t
ORIGIN      
        1 ctccaccacg caccggacag acgggtgcca gggcaatcgc atgggtaacg caggagccaa
       61 cgcgtatgcg ggctatgcgg aggggacgag catagatatg agacagtatg gtagttcttt
      121 gcagtcggtc ccggcaggcc ccgttcattc catcgcacgt ataacggcag atgcctcgcg
      181 tatggcctcg tcataggtca ttgcctggcc cgcggcccat tcgacgtcaa acgccccatc
      241 gccgagggct tcccgcgccg ccgcgacact tcgttcgtaa gccgctcgct cgttctccgg
      301 gaggggaacg cccgccgcag cgcgcaacgc cgctcccgcc cccaacagcc gagcggcgtg
      361 gtgcggctgc ttgtacgcta tcgcgagctc ggccacacca tcaagggtcg ccgtgaggtt
      421 ccagcgatcc ctgagctcgg cccagagcag gagcgcctct cgataatagg cggcagcctg
      481 aggaaaatcc ccctgatctc gggttacctc gccgagattg ccgaggtaga tggcaatgct
      541 ctgcgtgtcc ccggcctcgc gctggagcgc cagcgcctcc tcatagaggc tccgtgcgcg
      601 atcgtactcc tctcggtggt ccgcggtcac gccgagattg ttgaggacag tggccatcgc
      661 gcttttgtcg tcgagatcac ggaaccgttc cagcgcctcc tcgtgcagcg tatccgcccg
      721 ctcggaatcc ccttggagaa acgccacgat tcccagccgg ttcagcgtgt gagcggtgcc
      781 gcgctgaccg ccttccgtgt cgaggtcgac gagaatggcc agggcctgtt cttgcagccg
      841 gcgggcgccc tcgagatcgc cctgtgactg cgcgatgctg ccggcgttgc tgtgcgcttg
      901 ggcccgcgcg gcgggccgca cgccgtccat cggcagtgcg agcgcctgct cgagccagcg
      961 ccgtccctcg tcgagagagc cgcgcacgtc ccagagcttc tccatgctaa tgcccaggtc
     1021 cagcgccgtt tgcgcttcgg cccggtcgat ggcccaggtc agggccgcgc gcaggttgtc
     1081 gagctccagg tcgagccggt cgagccaatc tgcctggtcg gaaccccagg ttcctggtgc
     1141 ggcattcgtc gcgaaggtca ggaactgcgt ggcgtgggcg cgtcggaccg cttccgcttc
     1201 gccactcgcg aggagctgat ccaacccata ttcccggatc gtctccagca tcgtgtagcg
     1261 atcgcctcgg gcgtccgtgt cgatacgaat caggctcttg tcgacgagtg aggtgagccc
     1321 gccatcgaca tcgatgccgg tgtgatcggg ggggttgccc acggccaccg ctgcctcacg
     1381 caaccatcct ccggcgaaga ccgccagacg gcggaagaga gtctgctcat cccgcgacag
     1441 caggtcgtag ctccagtcga tggtgctgcg gagcgtccgc tgccgatcgg gcaggtcgaa
     1501 cgcaccatcg gtgagaagcg gcaggcgtgt ctccagccgg gctagcatct ccccaggtgt
     1561 caggtcggtg acccatgctg ccgcgagctc gatggccagc ggcagcccat cgaggcgatg
     1621 acatacctcg gcaatcgacc gggcgacggc gacattgacg atgaaatcgg gactgatagc
     1681 acgcgcccgg tcgacaaaga agcgggccgc ttcggcctgc atcagcgtgt cgatcggggc
     1741 tgccaaatcg ggaagcgcca ggggcggcac cgggaagccg cgctccgccc gcaggtgcag
     1801 ccgcatccgg ctcgttgcca gcaccgtgag ccccgggcat gccgcgagca gttgggcaat
     1861 caggctcgcc gcgggggcga cctgctcgaa gttgtccagc accaggagga gtctcttctc
     1921 tcgcaggtgc gcgatcagga gatcggcaag atcccgtcct gcctcttcct ggacgccgag
     1981 ctcgcgggcg atcgtggagg caaccagtgc cgggtcagtg accggcgcca gcgcgacgaa
     2041 cgtcacccca tggtcgaagt cgtcgatgag gtcggccgcg atagcgagcg ccaggcgcgt
     2101 ctttccggtc ccgccggggc ctgtcagggt gaggagacgc acactgcccg agcgaagctg
     2161 ggccgttgcc gccgccacct cccgctcgcg gccgaccagc ggcgtcggcg gctccggaag
     2221 gttgtgcgag cgggcatcga tcgtgttgag tggcgggaag gttgccggca gccccggcgc
     2281 gaggagctga tagatgcgcg cggggggcaa atcccgaagc cggcgctcgc cgaggtcacg
     2341 gagcgcgacg ccggaaggaa gcaggtcgcg aaccagctcg gccgtggccg ccgagagcag
     2401 cacctgcccg ccgtatccgg ctcccagcag gcggccaggc acgttgagga tcgcggtgcg
     2461 atagtcgccg ttcggctgcg gctcggcagc accggtatgg agcgccatcc ggctgcgcag
     2521 tggctcgggc gtctgccatt cctccgcaac cagctcccgt tgcgcctcca gcgcggcatc
     2581 gagcgcatcc ccggcactgg caaaggcagc ctgacagccg tcgccaacca ccttgtagat
     2641 cacgccgcgg tgcgcttcga cagctcgatg gagcagcagg tcgtgacggg cgtaggcatc
     2701 cggcatcgcc tcagcgtacc gccgccacag gcgcgtgctg ccttcgaggt cggtgaagag
     2761 gaaagtgacg ttaccgtgcg gcaggtcggg catgatggat tcctcgccgt gctcctctga
     2821 acgcgttgtc gccatgatag ggccggcggg gtagttcgtc ctgacctatt gcgttgaccg
     2881 ggggggttcc ctatgatgga tgggtcgcct ggagcgacaa gggtccctca cgacaacgcg
     2941 cgcgatatcc atccgagtga ggtgtgctat ggcaactgat cagctttccc ggctcgtgga
     3001 gcgggcaatg aacgatcccg ccttcatctc gcgagcgcag gccgaccttg acggcacgct
     3061 ggcggctgaa gacatctcgc tcgattccga cgagatggcg gcggtacgcg agttccactc
     3121 ccagatcgtc ggtctttccg aagaggaaat ccagggtcgg ttgagcgagt cgaaccgcca
     3181 acagggcccc atgtagccat tcgaccccga tgcggcatga cggccagatc actcacgcga
     3241 cactggactg gcttcaggcg ttcatcgaag ggatgtctgt tacgcccgag caggttgaat
     3301 tgctgagcct ggtcgtcgat gaggggcgct cccaggcagc atccttgccg aatggcctac
     3361 catccgcaga agtcacgatg gccgcctacg ccgccgcagg gggtggtgat gatttgcctc
     3421 tgcctgtcac ggcggcgtgt ctgtgtgtct atcttggggc ggatgtcctg gataatgtga
     3481 tcgaccgcga gctctcggat cgatgggagg agcacggagc gcatcaggca ctccttgccg
     3541 gtgtgacggt cttgacgcct ctctccatcg cctgccttgg cgagctgaat ggaccaatcg
     3601 agcggcgcct ggcagcccaa cgcatcctca tcgacacgct gattacaatg gcagccggcg
     3661 aggtggcgga cgtcgcgttc gaggggcgag atgatgtctc actcgacgcc tgcgaagcga
     3721 tggtgctcgc caagtcgggg gctgaatgct cactcttcgc tcaggtcggc gcgatcgttg
     3781 cgggtgcgcc ttcggacgtc tgcgacgcgt acgcgacctt cggccgagag ctcggcgccg
     3841 cgattcagct tgtcagcgat tgtgccgatc tcgccaccgg tgacgaaggc cgggatctgg
     3901 tgtcagggaa gcgtaccctg ccgatcgtcc acgccctgac ggttctgcca gatgccaagc
     3961 gagccgaact gcgggaccac ctcgccgcgt cggacgatga cgaacggcgg cgggccgcgc
     4021 gacagatgct ccttgacgcg ggatcgttcc acttt
//
LOCUS       DASYWL010000020         6594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_169907, whole genome shotgun sequence.
ACCESSION   DASYWL010000020 DASYWL010000000
VERSION     DASYWL010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6594
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169907"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VGR22_00750"
     CDS             complement(<1..446)
                     /locus_tag="VGR22_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127130.1"
                     /translation="MAQVIEAPRAEPRGPGHDDNGHGHAEHPPTSLGMDHRKLLMWLF
                     LASDCMFFGALIATYMIYRGRAEEVGVGPFPHELVDIPFTSVSAFVLLMSSLTMVLAL
                     GSLQQGNVRNSRIWLFATALLGSIFLGGQYFEFTEFYHEGLGITTN"
     gene            complement(567..2531)
                     /gene="ctaD"
                     /locus_tag="VGR22_00755"
     CDS             complement(567..2531)
                     /gene="ctaD"
                     /locus_tag="VGR22_00755"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEV2127131.1"
                     /translation="MASIAQQPQPTTVERPVTPARSRVWDWLTTIDHKKIGILYGVSA
                     IFFFLVGGFEALLIRAQLIRPLNGIVGPETYNELFTMHGTTMIFLAVMPLSVAFFNYI
                     MPLQIGARDVAFPRLNAFSYWVFLFGGILLNASWFVGGFPGEVPNQGWYGYAPLTELA
                     WNPTRAVDFWVLGLLVLGVSSVAGALNFTVTILNMRAPGVTLMRMPIFTWSTLIVSIL
                     IFMAFPAITIALFLLAMDRFVGTGFFEAAAGGDPLLWQHLFWVFGHPEVYILILPAFG
                     VISEIIPTFSRKPLFGYAVMVYAIAAIAFLGFGVWAHHMFTTGLGPTANSVFAASTML
                     IAIPTGVKIFNWIGTMWLGQLRFTTAMLFAVGLVSQFVIGGLSGVMHAVVPVDTQQND
                     SYFVVAHFHYVLFGGSIFGLIAAIYYWFPKFTGRMMDERLGKWNFWTTFLGFNMTFFP
                     MHFLGLEGMPRRYYTYAEGSGWAFWNLISTIGAFILGASILLFIYNIARSLQTGERAG
                     ANPWDAGTLEWATSSPPSVYNFAVVPTIEHRDPLWAEKYGLEEEEEDLEITIAGKETG
                     HAHVPDENRREQTLMAAVEADTGTADIHMPNPSYYPLIAAIGFTLVAVGLLFENVDIP
                     IGALPVPIITLVGVLILVGGIVGWSLEPPD"
     gene            complement(2583..3848)
                     /gene="coxB"
                     /locus_tag="VGR22_00760"
     CDS             complement(2583..3848)
                     /gene="coxB"
                     /locus_tag="VGR22_00760"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEV2127132.1"
                     /translation="MAQTTRSTSSRKGGLRRVLTLGALAIVALAVLAACGIEEPNPYS
                     HVSPESPTAEDIHSLYKLVFWLALIVFVGVQFAIVYTAMRFRRRSAARRRPPQIHGNS
                     RLEITWTVIPAVILLIILVPTITTLFAHGEAAEEGDLVVEVNGKQWWWEVIYNEDAYG
                     NDLGVVTANHIRVPVGQETIFRLKSNNVIHSFWVPRLSGKMDVIPGHETELSITPLEP
                     GVYHGECAEFCGIQHAWMRFEIHAMPEDQFYGWINAMRAGNPGTTNQDVELPEGVTRA
                     PETMNVCLACHNIDGFEGSAGLAGMQAPRTFGPNLTNLACRDSIAAGLLINTPENLRV
                     WIDNPGGVKPGNYMATQLQPGTFTDEQLDELVDYLLTLRPPGGCEDFLADPVGADVTA
                     EGDLAATPADDTEVAVATPDVTPVDDDED"
     gene            4352..4609
                     /locus_tag="VGR22_00765"
     CDS             4352..4609
                     /locus_tag="VGR22_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127133.1"
                     /translation="MRNPLLASRAMAVALLLIGLGLMVLALVADAVDIGGGRGFGYQQ
                     LIVFIAGMTVVLLAGAILLQPRLSQNDDTSYRDEQDPDRHR"
     gene            4713..>6594
                     /gene="argS"
                     /locus_tag="VGR22_00770"
     CDS             4713..>6594
                     /gene="argS"
                     /locus_tag="VGR22_00770"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEV2127134.1"
                     /translation="MSTTSPPPTPAGIIAREETAAADAIRQAAIAAGANPGGRPVDLR
                     PLPFAGTWGSASTIAHMLASGLVQQEMEAEGKLEGLSKKQIKKLVSGQASERAQELAE
                     RIAETVRESGRFASVEAVNGYINISYDAASVARQLLGEVLEQDERYGRVPEGTHPSRV
                     MIEHSQLNTHKAAHVGHLRNICLGVAVTNISEAAGFETMPVTYIGDIGRHVIQCLWCY
                     ERFHASEEPHSRSSRGRWLGSIYAESSARLRYRKDLLAFLNTLIKEDPAFVEAIDRML
                     KQLWEVHKEGEDIAYLLGCVINGNEIDPARFVDDEVIVRFWPIVGEQLRAERDNPPAE
                     ADGSQPAMDGETAEPSMTPQERVEAWEQLNAHIDWWRHADEWEQEVKATFQRWEQKDP
                     EFVALWEKTREWSLIELREIFDEFGATFDEWFYESGVEDAGREIVKDLLEQGIAEISD
                     GLPVVKIDEKLGLEQETYRTLPILRSDGTTLYSTKDLALTRMKFVDYNVDTAVWVVDV
                     RQSLYFNQISKILELSGFEQAKNTVHLGYEFVALPTGAISSRKGNAPMLEDVRNAVLE
                     RAQGIIEEKNPSMDTGKREQVAWQVGIGAIKYTMLARDGNKVIVFDPEEALSFDGHAA
                     PYI"
BASE COUNT         1295 a         2093 c         2102 g         1104 t
ORIGIN      
        1 atgttggtcg tgatgcccag cccctcgtgg tagaactcgg tgaactcgaa gtactggcca
       61 ccaaggaaga tcgatccgag gagcgctgtg gcgaagagcc agattcgcga gttgcggacg
      121 ttgccctgct gcagcgagcc aagggcgagc accatcgtca gcgagctcat cagcagcacg
      181 aaggcactga ccgacgtgaa cgggatatcg acgagctcat gcgggaaggg gccaacgccc
      241 acttcctctg cgcgaccgcg atagatcatg taggttgcga tcagcgcgcc gaagaacata
      301 cagtccgacg ctaggaacaa ccacatcagc agcttgcggt gatccattcc cagactggtg
      361 ggtggatgtt ccgcgtgacc atgcccgttg tcgtcgtgcc ccggcccgcg gggctcggct
      421 cgcggcgcct cgataacctg tgccatgctc tccccttgcc gttttcgtgg gactcgggcc
      481 caactgttcg accctggtgg cacccgacgc atgtgatcgt gcgctggagc gaggcgggtg
      541 cctcctcgct ccagcaagtt cgatgcctaa tccggcggct ccagcgacca gcccacgatg
      601 ccgccgacca ggatcagcac gccgactagc gtgataatcg gcaccggcaa cgcaccgatt
      661 gggatatcca cgttctcgaa gaggagcccg actgcgacga gcgtgaagcc gatcgctgca
      721 ataagcgggt agtacgaggg gttgggcata tggatatcag cggtgccggt gtcggcctcg
      781 acggcggcca tgagcgtctg ctcgcgccga ttctcgtcgg gcacatgtgc atgcccggtc
      841 tccttgccag caatcgtgat ctcgaggtcc tcctcctctt cctccaggcc gtacttctcg
      901 gcccagagcg gatcacggtg ctcgatggtc ggtaccacgg cgaagttgta gaccgacggc
      961 ggggaggagg tcgcccactc cagcgtgccg gcgtcccacg ggttcgcacc cgcacgctca
     1021 ccggtctgca acgaccgtgc aatgttgtag atgaagagca gtatggaggc accaagaatg
     1081 aacgcgccga tggtggatat gaggttccag aacgcccacc cactgccctc ggcataggtg
     1141 tagtagcggc gcggcatgcc ttccaggccg aggaagtgca tcgggaagaa ggtcatattg
     1201 aagccgagga acgtcgtcca gaagttccac ttgccaagtc gctcgtccat catgcggccc
     1261 gtgaacttcg ggaaccagta gtagatcgcg gcgatcagac caaagatcga cccaccgaag
     1321 agcacgtagt ggaagtgcgc caccacgaag tagctgtcgt tctgctgggt atcgaccggc
     1381 acgacggcgt gcatgacacc cgacaggcca ccgatcacga actgggatac cagcccgacg
     1441 gcaaagagca tcgccgtggt gaaccggagc tgcccgagcc acatcgtgcc gatccagttg
     1501 aagatcttca cgccggtcgg aatcgcgatg agcatggtcg acgccgcgaa caccgagttc
     1561 gccgtcggcc caagaccggt cgtgaacatg tggtgcgccc acacaccgaa gccgaggaag
     1621 gcgatggcgg cgatcgcgta gaccatcacg gcgtagccga agagtggctt gcgcgagaag
     1681 gtcgggatga tctccgagat gacaccgaat gccggcagga tcaggatata gacctccggg
     1741 tgcccgaaga cccagaacaa gtgttgccac aggagtggat cacccccggc agcggcctcg
     1801 aagaagccgg tgccgacgaa gcggtccatc gcgagcagga agagcgcgat cgtgatcgcc
     1861 gggaaggcca tgaagatcag gatcgagacg ataagcgtgg accaggtgaa gatcggcatg
     1921 cgcatcagcg tcacaccggg tgctcgcatg ttgaggatgg tgaccgtgaa gttgagcgcg
     1981 ccggcgacgg aactcacgcc cagcacaagc agaccgagta cccagaagtc gacggcgcga
     2041 gtcgggttcc aggccaactc cgtcagcggc gcgtaaccgt accacccctg gttcggcacc
     2101 tcgccaggga atccgccgac gaaccagctg gcattgagca gaatgccgcc aaagaggaag
     2161 acccagtagc tgaaggcgtt cagccgtggg aaggcaacat cacgggcacc gatctgcagc
     2221 ggcatgatgt agttgaagaa cgccacgctc aacggcatca ccgccaggaa gatcatggtg
     2281 gtgccatgca tcgtgaagag ctcgttgtag gtttcgggcc ccacgatgcc gttgagcggc
     2341 cggatgagct gcgcgcggat gagcagcgcc tcgaatccgc ccaccaggaa gaagaagatg
     2401 gcggagacgc cgtacaggat gccgatcttc ttgtgatcga tcgtggtcaa ccagtcccac
     2461 accctcgatc gtgctggtgt tactggccgt tctaccgtgg tgggctgcgg ctgctgggcg
     2521 atcgatgcca tcactcgctc ctgttgtcaa tcggtgctgc gaacgcgacc gggggggatc
     2581 cactaatcct catcatcgtc aaccggtgtt acatcggggg tggccacagc cacttcggta
     2641 tcgtcggccg gggtcgccgc caggtcaccc tcagcggtta cgtctgcgcc aaccggatcg
     2701 gcaaggaagt cctcacatcc accgggcggc ctgagcgtca gaaggtagtc gaccaactcg
     2761 tcgagctgct catccgtgaa cgttccgggc tggagctggg ttgccatgta gttgcccggc
     2821 ttcacccctc ctgggttatc gatccacacc ctgagattct cgggagtgtt gatcagcaag
     2881 ccggcggcaa tcgagtcgcg gcaggcaagg ttggtcaggt tcggaccgaa cgtgcgcggt
     2941 gcctgcatgc cagcgagacc ggcgctgccc tcgaagccat cgatattgtg gcaggcgagg
     3001 cagacgttca tcgtctccgg cgcgcgggtg accccctcgg gcagttcgac atcctgattg
     3061 gtggtgccgg gattgccagc gcgcatcgcg ttgatccagc cgtagaactg atcctctggc
     3121 atcgcgtgga tctcgaagcg catccacgca tgctgaatgc cgcagaactc ggcgcactcg
     3181 ccatggtaca cacctggctc cagcggcgtg atcgagagct cggtttcgtg gccggggatt
     3241 acgtccatct tgccgctgag acgcggcacc cagaaggagt ggatgacgtt gttcgacttc
     3301 agcctgaaga tcgtttcctg gccgaccggt acgcggatgt ggttcgcggt cacgacgccg
     3361 aggtcgttgc cgtaggcatc ctcgttgtag atcacctccc accaccactg cttgccgttg
     3421 acctcgacga cgagatcgcc ctcttcggct gcttccccgt gggcgaagag tgtggtgatg
     3481 gtcggcacca ggatgatgag caggatcacg gccgggatca ccgtccaggt gatctccagg
     3541 cgggagttac cgtgaatctg tggcggccgg cggcgcgccg cgctcctgcg ccggaaccgc
     3601 atcgccgtgt agacgatcgc gaactgtacg ccgacgaaaa cgataagcgc aagccagaac
     3661 accagcttgt acagcgaatg gatatcttcg gccgtcggcg attccggact aacgtgcgag
     3721 tacggattcg gttcctcgat cccgcaggcg gccagaactg caagcgccac gatcgccagg
     3781 gccccgaggg tgaggacccg acgcaggccc cccttgcggc tcgaagtcga tcgggtggtc
     3841 tgggccatcc cgttgtcact ccgcccgttc attctgcctc ccgcaccccg tccgggcctg
     3901 ccagacgttg aagctaaaca ccagaacagc gccaccaccc cacgaatggc gatggtgcgc
     3961 ccatcgtaca cgttgccccg tatgctaccc gactttcacg cgtcacgcgg tctgcaagcc
     4021 tggggattgc gaagagcgac gctgtcctct cctgcgtgcc ggtcgcatca cgatgctcgg
     4081 cccataccgg agatcggatg ccattcggct gccggcgagt gtcgccagtg tcgccatcgg
     4141 tccgctctcc cccggatcgg gtcgttgcgt gaacatatag cggacactgc cgacgacggt
     4201 caagcactcc tcgccgggtt tggaccgggg aattggtata cttgccgcac tagcatcggc
     4261 gcgtcgcaac accaggcgtg tcacggttcg cgatcgacac agagccggcc gatcgctcat
     4321 tgttgtgcat ttccgagctg aggtggtccc cgtgcgcaac ccccttttgg catcacgagc
     4381 aatggccgtc gccctgctgc tgatcggcct cgggctgatg gtgctggcgc tcgtggctga
     4441 cgccgtagac atcgggggcg gccggggctt cggctaccag cagttgatcg tcttcatcgc
     4501 cggcatgacc gttgtgctgc tcgccggcgc catcctgctg cagccccggc tttcccagaa
     4561 cgatgacacc agctaccggg acgagcagga ccctgaccgc caccgctgat gccgagccat
     4621 gcacgctggt gtcctcaccg cgcatcaccc gaacaccaca cggggcggac acgatctata
     4681 ccccccaatc ccaaaggaga gttgaccacc ccatgagcac tacgtcaccc ccccccaccc
     4741 cggcaggcat catcgcgcgg gaggagaccg ccgcggccga tgcgatccgc caggcggcca
     4801 ttgctgccgg tgccaatccc ggcggtcgac cggtcgacct gcgcccgctg ccgttcgccg
     4861 gcacgtgggg cagcgcctca accattgccc acatgctcgc cagcgggctg gtgcagcagg
     4921 agatggaggc cgagggcaag ctcgaggggt tgagcaagaa gcagatcaag aagctcgtca
     4981 gcggccaggc cagcgaacgc gcgcaggaac tggccgagcg gatcgccgaa accgtgcgcg
     5041 agagcggccg gttcgcatcg gtcgaggctg tcaacggcta catcaacatc tcctacgacg
     5101 ccgcctccgt cgcccgacaa ctgctcggcg aggtactgga gcaggacgaa cgttacggcc
     5161 gcgtgccgga gggcactcac cctagccgcg tgatgatcga gcactcgcag ctcaacaccc
     5221 acaaggccgc ccacgtcggt cacctgcgca acatctgcct cggtgtggcg gtgaccaaca
     5281 tctccgaagc ggccggcttc gagaccatgc cggtcaccta tatcggcgac atcggccggc
     5341 acgtgatcca gtgcctctgg tgctacgagc gcttccacgc cagcgaggag ccgcatagca
     5401 ggagctcgcg cgggcgctgg ctaggcagta tctacgccga atcgtccgcc cggctgcgct
     5461 accggaaaga cctgcttgcc ttcctgaaca cgctcatcaa ggaagacccg gccttcgtcg
     5521 aggcgatcga ccggatgctc aagcagctct gggaagtaca caaggagggc gaggacatcg
     5581 cctacctgct cggctgtgtc atcaacggca acgagatcga tcctgcccga ttcgtcgatg
     5641 acgaggtgat agtgaggttc tggccgatcg tcggcgagca gcttcgcgcc gagcgcgata
     5701 acccgcccgc cgaggcggac gggagccagc ccgccatgga tggagagacc gccgagccgt
     5761 ccatgacgcc gcaggaacgg gtcgaggcgt gggagcagct caacgcccat atcgactggt
     5821 ggcggcatgc agacgagtgg gagcaggagg tcaaggccac tttccagcgc tgggagcaga
     5881 aggaccccga gttcgtcgcc ctgtgggaga agacgcgcga gtggagcctg atcgagctgc
     5941 gagagatctt cgacgagttc ggcgccacct tcgacgagtg gttctacgaa agcggcgtcg
     6001 aggatgccgg ccgcgagatc gtgaaggacc tgctggagca gggcatcgcc gagatcagcg
     6061 acggcctgcc ggtcgtcaag attgacgaga agctgggcct cgagcaggag acctaccgca
     6121 cgctgccgat cctgcggtcg gacggcacca ccctctacag caccaaggac ctggcgctga
     6181 cccggatgaa gttcgtcgac tacaacgtcg acaccgccgt ctgggtggtg gacgtgcggc
     6241 agtcgctcta cttcaaccag atctcgaaga tcctcgagct ctccgggttc gagcaggcaa
     6301 aaaacaccgt ccatctcggc tatgagttcg tggcactgcc gaccggcgcg atctcctcgc
     6361 ggaagggcaa cgccccgatg ctggaggacg tccgcaatgc cgtgctggag cgcgcacagg
     6421 ggatcattga agagaaaaac ccgtctatgg acaccgggaa gcgcgagcag gtcgcgtggc
     6481 aggtcggcat cggcgcgatc aagtacacga tgctcgcccg cgacggcaac aaggtgatcg
     6541 ttttcgatcc ggaggaggcg ctctccttcg acggtcacgc cgcgccctac attc
//
LOCUS       DASYWL010000021        12644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_301192, whole genome shotgun sequence.
ACCESSION   DASYWL010000021 DASYWL010000000
VERSION     DASYWL010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 12644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12644
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301192"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="VGR22_00775"
     CDS             <1..1088
                     /locus_tag="VGR22_00775"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV2127135.1"
                     /translation="WKLEDREQWRSLMLDMDTLSQLNELDSALAMLGSIGSVMALSDA
                     AIERVSDDLPEDLGRWLDGWAEAAGDLRQQREVESESARLSLPPSVVAAIGKDLLRRL
                     FRPAIARVPGEHEASVAGTACDSGETTRAWEPGRPLDLNLVGTLANAIRREGPTDKGQ
                     IRLRLEDFVVVERTARTAMSTVLALDRSHSMGQSGAWASAKKVTLAMHELIRQSYYRD
                     ALSVLAFSSSAETVAIAELPEMHWDRFEHGTHLQAALALGRRMLRGSAAATKQIVVIT
                     DGEPTIASTVGVEIFASPPTTNVLEATMREVIRCTRERIVINMVILGEMGAAAFAEQV
                     ARLNGDRIFDASNQDLGTFIFRDYLST"
     gene            1187..3403
                     /gene="purL"
                     /locus_tag="VGR22_00780"
     CDS             1187..3403
                     /gene="purL"
                     /locus_tag="VGR22_00780"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF002290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEV2127136.1"
                     /translation="MAITEGQWREVALSDDEYRRIVELLGREPSLVELGMFGAMWSEH
                     CGYKHSRPHLKQLPTEAPWVLQGPGENAGAVDIGNGLAAVFKVESHNHPSAVEPYEGA
                     ATGIGGIVRDIFTMGARPIAILDALRFGALDRPRNQYLFSHVVAGIGGYGNCLGIPTV
                     GGETAFDSRFDGNPIVNAMCVGIVPHDGIVRAKASGVGNRIVLIGAKTGRDGLHGAMF
                     ASVDDPETSHRGVIQVGNPFLEKQLMEACLELLQTDTVVAMQDLGAAGMTSSIVEIAS
                     RGGVGADVDVLKVPRREEGMTPYEIMLSESQERMVLVCAPDQVTNVIRVLDRWSLAAS
                     DIGTVTDDGLVIVREGDEVVCKVPVGIFVDECPTYHVTASEPEVTRTRREASIDPDVV
                     ASEARVADELAALIAHPNLGTRRSIWEQYDHTILTNTVVGPGGDAAVLRVKGTPLGVA
                     LSIDCNSRYCDLDPYNGARLAVAEASRNVSCAGAKPLGITNCLNMGNPEQLPGSWHLQ
                     EAVRGIADACRDLGVPVVSGNVSLYNESNGVAIQPTPMIGCVGVLDDVTRVKHHAFQD
                     GDDLILVSSGEPTLGGSEWLSIRGGRSEGTPPSLDMATEARLQTLLRDLATRDRVHSV
                     HDISQGGLAVAVAEGALAGDIGADLSPGEAGGRHEILRWFGEYGSAAIIAAEPSLRAA
                     IFDKAESAGLDATMIGRAIGDAVIFPDGSSVSLASLRTASESALVSALETLELVSA"
     gene            3445..4902
                     /gene="purF"
                     /locus_tag="VGR22_00785"
     CDS             3445..4902
                     /gene="purF"
                     /locus_tag="VGR22_00785"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEV2127137.1"
                     /translation="MLPAEQPQLDGDTLHEECGVFGIVAPGEDVARISYFALYALQHR
                     GQESAGIATFDGTQLHAHHGMGLVAQAFDEESLADLPGTMAIGHTRYSTAGSNRADNA
                     GPMITDSDIGPVAVSHNGNLTNALELREELEAEGEEFVTTTDSEVLARVIARAPGLTI
                     VDRIRNAMRRLVGAFSLAVLAEGRVFAVRDPYGVRPLSIGTLNGHKVVASETCAMMTI
                     GAEVDREVEPGEIIEIREGEILSHGATPADREALCLFEMIYFARPDSEIHGKRLHQVR
                     QAMGRELYREHPVDADVVIGLPDSATPATIGYAKESGIPYAEALIKNRYIGRTFIQPN
                     QMLRERGVSLKFNVVPEVVEGKRVILVDDTIVRGTTSRPIVNLIREAGAREVHMRVHA
                     PPIMWPCYLGVDLARKEELIAARLSVPEIGQELGVDSIAYLSLDGLFRAIGESGSGYC
                     TGCLTGNYPVPAFTSLDKLALEHRSVQRAKVGVRA"
     gene            4899..6023
                     /gene="gcvT"
                     /locus_tag="VGR22_00790"
     CDS             4899..6023
                     /gene="gcvT"
                     /locus_tag="VGR22_00790"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEV2127138.1"
                     /translation="MTTSATDRRTALYQQHVDLGARMVSFAGWEMPIQYGGILGEHKA
                     VRTAAGLFDLGHMGQVNFTGPDALEFLQWTTTNDVSSLGPGDAQYALLPNEQGGVVDD
                     IIVYRRPEGEAGFLVVVNAANRERDVAWWQELLSRRPDLDVTVRDVSDETGMIAIQGP
                     RAEAITAKVTDADLPSIENFSWRRGTVAGKNAMIARTGYTGEDGFEFYAANQNIAAIW
                     DALLKAGEGDGLVPVGLGARDTLRLEARMPLYGQELGDDISPYEAGLGWAVKTDAKDF
                     VGKEPIAQVKADKPKRRTVGFATVGRTSAPRSHCRVLVDDEEVGYVTSGAFSPTLQQN
                     IGLALINRAYAGVGKPLQIEVRGKVVDAEQVKTPFYKRAE"
     gene            6106..6495
                     /gene="gcvH"
                     /locus_tag="VGR22_00795"
     CDS             6106..6495
                     /gene="gcvH"
                     /locus_tag="VGR22_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEV2127139.1"
                     /translation="MAAPENLKYTKTHEWVKVDQDTVTLGITDAAQTELGDITYLELP
                     EAGDQLSSGEPFGVVESVKAASDIYSPIDGEVVERNDDAVESPELINQSPYEKAWMIK
                     VKVSDPSQLESLLSSSDYDAFASESLG"
     gene            6518..7849
                     /gene="gcvPA"
                     /locus_tag="VGR22_00800"
     CDS             6518..7849
                     /gene="gcvPA"
                     /locus_tag="VGR22_00800"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEV2127140.1"
                     /translation="MSFNPHTPEDRERMLATIGVASTDDLFTPVPETIRYPHLDLPPA
                     LTELEAIRRLEHLAEKNFVPDAEDVYLGAGSYRHFIPSTVDHLIQRGEFYTAYTPYQP
                     EVAQGTLQVIYEFQTMVAELLGFEVANASMYDGATAMAEGALTAVSASRKREKIVVSP
                     TVHPAYRKVLETYVEGTAVTSETLESADPFRFDVSALEGRLDDSTACVVIQYPNFFGT
                     IEDVQAIADAVHEAGALLVVNTSPVPLGLLKPPGALGADVASAEGQPLGIPPGYGGPY
                     VGLLATGKKHVRQMPGRLAGVTADAEGKRGYVLTLQTREQHIRREKATSNICTNQGLM
                     ATAATIYMSTLGPEGFREVATRSYQNAHYLADQIVKREGYAVANTGEFFHEFVVECPE
                     PAASVNRRLLDHGIVGGLDLGTVDDSLKNFMLICATELHSRQSIDRFVEAI"
     gene            7968..9320
                     /gene="der"
                     /locus_tag="VGR22_00805"
     CDS             7968..9320
                     /gene="der"
                     /locus_tag="VGR22_00805"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEV2127141.1"
                     /translation="MPKPIVALVGRPNVGKSTLFNRLIGERRAIVQNEPGTTRDRVYG
                     STDWNGVDFTIIDTGGLQEDSDLPRDETEVRRLIARQTRNQATMAISEADLIVMLVDA
                     RTGPTPQDQEIADLLRRSDKPVVLGANKAERNDLRFEALEFYNLGLGEPIPISAYHGN
                     GTGDLLDRVVEELPNAEELEEDEVEGPKIAIVGRPNVGKSRLLNALLGEERAIVSEEP
                     GTTRDSLDTIIEWEGEQLTLIDTAGIRRRGRVEEGIERFSVMRSMRAIDRADVVLLVI
                     DATEDFTAQDLHIAGYIEEQKKGIVVVVNKWDLVEKDSRSMDEYRERARRELDFMPYA
                     PLVFISAKTGQRVGKVLEMALGVIEERNRRVNTAALNRMLKDAVTKHPPPSKPGKWVR
                     FFYATQADTAPPTFVFFCNDPKAIHFSYRRYLENELREAFDFSGTPLRISFRSRHEPS
                     "
     gene            9323..9940
                     /gene="plsY"
                     /locus_tag="VGR22_00810"
     CDS             9323..9940
                     /gene="plsY"
                     /locus_tag="VGR22_00810"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEV2127142.1"
                     /translation="MPLIWSILGIAAAYLSGSIPWGVVVGKLIKGIDIRKIGSGNTGA
                     TNAMRAFGWRIALLVAVLDVLKGLVPVLVARWLDAPTWTVALMAVAATAGHCWSPFMK
                     FRGGKGMATGGGATIGLLPWLLTMLPLMIIIVLITRYVSLASIIAMITGTTIAYIFAA
                     TTPFSWVWATAVLGISAIVVGKHHGNIMRLLTGTERRFGVPESSS"
     gene            complement(9956..10933)
                     /gene="galE"
                     /locus_tag="VGR22_00815"
     CDS             complement(9956..10933)
                     /gene="galE"
                     /locus_tag="VGR22_00815"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV2127143.1"
                     /translation="MHVLVTGGGGYIGSIAVERLVARGDRVTVLDSFVRGHRQAVHPD
                     AAIVECDIRDAVATRDAVQRLGAEAIMHFAALQLVPESVKEPGAYFATNTSGGLHMLE
                     AARDAGIQRFVFSSTAAVYGEPETVPIPENSPKLPINPYGQSKWMVEQMLEAFARQHG
                     LNHAIFRYFNVAGASEQRGEDHNPETHVIPVALQALLGQRDGFVVYGTDYDTADGTAV
                     RDYVHVIDLVDAHIAALDRLDEPLGAMNLGTANGFSVQQIVDAVQWVTGRELPVTYGA
                     RREGDPATLIADSTRAREVLDWRPAHSTLDEMIGSAWEWLQRHPEGYGS"
     gene            11099..12364
                     /gene="glyA"
                     /locus_tag="VGR22_00820"
     CDS             11099..12364
                     /gene="glyA"
                     /locus_tag="VGR22_00820"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEV2127144.1"
                     /translation="MTASEKLAQYDPEVAAAIRHEEQRQREGIELIASENYVSEAVLT
                     AVGSVLTNKYAEGLPGKRYYGGCEYVDIAEELAIDRAKTLFGAEHANVQPHSGASANL
                     AAFFSLMDPGDTILGMNLSEGGHLTHGSPVNFSGRFFNVHAYGVDHQTGTIDYDAVLE
                     KAKEVRPRGIICGASAYSRIIDFKRFREIADEVGAYLFADVAHIAGLIVAGEHPTSIS
                     HAHITTTTTHKTLRGPRAGLILTSEEHAKTVDKTVFPGMQGGPLMHVIAGKAVAFQEA
                     LQPSFKTYAKQIIANARAMASALQERGLHIISDGTDNHLFLVDVGEKGISGRKAERVL
                     DSVGISANRNTIPGETRPPTQASGLRIGTPAMTTRGFREQESVQVADMIARVIEAPED
                     EQVLDTIRNEVREMTATYPVPGTTPSASK"
     gene            complement(12438..>12644)
                     /locus_tag="VGR22_00825"
     CDS             complement(12438..>12644)
                     /locus_tag="VGR22_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127145.1"
                     /translation="RWRRHCSMKSDNSVLTLVFASIGAATVGFVLGAALGRSLVHLLS
                     VVWAFIDRRDRSDDERLRFELLLQ"
BASE COUNT         2381 a         4062 c         4094 g         2107 t
ORIGIN      
        1 gttggaagct cgaagaccgc gagcagtggc ggtccctgat gctggatatg gataccctgt
       61 cgcagctcaa cgagctggac agcgcccttg cgatgctcgg atcgattggc agcgtgatgg
      121 ctctctccga cgccgcgatc gaacgcgtct cggatgacct gcccgaggac ctgggcaggt
      181 ggctggatgg ctgggccgag gcagcgggtg acctgcgtca acagcgcgag gtggaatcgg
      241 aatcagcgcg actgtcgctg ccgccatccg tcgtcgcggc aatcgggaag gatctgctca
      301 ggcgcctctt ccggccagcc atcgcccgag tccccggtga gcacgaagcc tcagtcgccg
      361 gaaccgcttg cgactccggc gagacgaccc gggcctggga gccgggacgg ccgctggacc
      421 tcaatctggt cggaaccctc gccaatgcca tccggcgtga aggtcctacc gacaagggtc
      481 agattcggct acggctggaa gatttcgtgg tggtcgagcg cacggcacgg accgcgatgt
      541 ccaccgtgct ggcgctcgac cgcagccact ccatggggca gagcggggca tgggcatccg
      601 ccaagaaggt gacgctcgcc atgcacgagc tgatccgtca gtcatactac cgcgacgcgc
      661 tatcggtgct ggccttttcc tcgtcagcgg agaccgtggc gatcgccgag ctaccggaga
      721 tgcactggga tcgctttgaa catggcacgc atctgcaggc ggccctcgca ctcggccgcc
      781 gcatgctgcg ggggtccgcg gctgccacga aacagatcgt cgtcatcacg gacggcgaac
      841 cgactatcgc ctccaccgtg ggtgttgaga tcttcgcctc tccacccacg accaacgtgc
      901 tcgaagcgac catgcgggag gtgatccgct gcacgcgcga gcggatcgtc atcaacatgg
      961 tgatcctcgg tgagatgggt gcggcggcct ttgcggagca ggtggcgcgg ctgaacggtg
     1021 accgaatctt tgacgcatcg aaccaggatc tcggtacgtt tatcttccgg gactatctct
     1081 cgacatagaa gtacgttcac ccggttgcga ttgcgttaca gtagggatga cacaccgatg
     1141 acctcgccgg atggcgaggt cgatccccgc gctggagttt gcccacgtgg cgataactga
     1201 gggtcagtgg agagaggtcg cactcagcga cgatgagtac cgccggatcg tcgagctgct
     1261 cggccgggaa ccatcgctcg tcgagctcgg catgttcggc gcgatgtgga gcgagcactg
     1321 cggctacaag cactcccgcc cacatctgaa gcaactgccg accgaggccc cgtgggtgtt
     1381 gcaggggccc ggcgaaaacg cgggcgcggt cgatatcggc aacggcctgg cagccgtctt
     1441 caaggtcgaa tcccacaatc acccgagcgc ggtcgaaccg tacgagggtg ccgcgacagg
     1501 catcggtggc atcgtccgcg acatcttcac catgggcgct cggccgatcg cgatcctcga
     1561 cgcgctccgg ttcggcgcac tcgacaggcc gcgcaaccag taccttttct cgcatgtggt
     1621 cgccggtatc ggagggtacg gcaactgcct cggcattccg acggttggcg gtgagacggc
     1681 cttcgacagc cgcttcgacg gcaatccgat cgtgaatgcc atgtgcgtcg gcatcgttcc
     1741 tcacgatgga atcgttcgag ccaaggctag cggtgtgggc aaccgcattg tgctgatcgg
     1801 cgcgaagacc ggtcgggacg ggctacacgg cgcgatgttc gcctcagtcg acgacccgga
     1861 aacgtcccat cgtggggtca ttcaggtcgg caacccgttt ctcgagaagc agctcatgga
     1921 ggcctgcctc gagctgctcc agaccgacac agtcgttgcg atgcaggatc tcggtgcggc
     1981 gggcatgacg agctccatcg tcgagatcgc cagcagaggc ggcgtcggcg cggacgtcga
     2041 cgtgctgaag gtgccacgcc gcgaggaggg catgaccccc tacgagatca tgctctcgga
     2101 gagccaggag cggatggtgc tcgtctgcgc acccgaccag gttacgaatg tcatccgcgt
     2161 gctcgaccgt tggtccctgg ccgcgagtga catcggcacg gtgacggatg acgggctggt
     2221 gatcgttcgc gaaggtgacg aggtcgtctg caaggtgccg gttggtatct tcgtcgacga
     2281 gtgtccgaca taccacgtga cagccagcga gccggaggtg acccggacgc gacgcgaggc
     2341 gagcatcgat ccggacgtgg tcgcatcgga ggcaagggtg gcggatgagc ttgcggcgct
     2401 gatcgcccat ccgaatctgg gcacgcgccg ctcgatttgg gagcagtacg accacacgat
     2461 tctcaccaac accgtcgtcg gtccgggcgg cgatgcggcg gtgcttcgcg tcaagggcac
     2521 gccgctcggt gtggcgctct cgatcgactg caactcccgg tactgcgatc tcgatcccta
     2581 caacggcgcg cggctcgccg tcgccgaggc atcgcgcaat gtgtcctgcg cgggcgcgaa
     2641 gcccctcggc atcacgaact gtctgaacat gggcaatccc gagcagctgc ccggcagctg
     2701 gcacctgcag gaagccgtgc gcggcatcgc ggacgcctgc cgggatcttg gcgtgccggt
     2761 ggtaagcggc aacgtcagcc tgtacaacga aagcaacggc gtggcgatcc agccgacgcc
     2821 aatgatcggc tgcgttgggg tgctcgacga tgtaacgcgt gtgaagcacc acgcctttca
     2881 ggatggagac gatctcatcc tggtcagctc tggtgaaccg accctcggcg gcagtgaatg
     2941 gctgtcgatc cgcggcggcc ggagcgaggg gacacctccc agccttgaca tggccaccga
     3001 agcgcgattg cagaccctgc tgcgagacct cgcgacacgg gaccgcgtcc actccgtcca
     3061 cgacatctcc cagggtggac tcgcggttgc cgtcgctgaa ggagcgctgg ccggcgatat
     3121 cggtgctgat ctctcgccgg gagaagccgg agggcgccac gaaattcttc ggtggttcgg
     3181 agagtatgga agcgctgcga tcatcgctgc cgagccgtcc ctgcgggcag ccattttcga
     3241 caaggcagag agcgcagggc ttgatgcgac catgatcggt cgcgcaatcg gcgacgcagt
     3301 gatcttcccc gatggctcgt ccgtgtcgct cgcgagcctc cgtactgcca gcgagtcagc
     3361 gctggtgagc gcactcgaaa cattggaact ggtgtcggca tagtgcagcg ggtctgacca
     3421 gagactcttg gcgaaaggta agccatgctt ccagctgagc agccacagct tgacggcgac
     3481 acgctccacg aagagtgcgg ggtgttcggc atcgtcgctc ctggcgagga tgtcgcgcgg
     3541 atctcgtact ttgcgctgta tgccctgcag caccgcggcc aagagagcgc cggcatcgcg
     3601 accttcgatg gtacccagct ccatgcccat cacggcatgg gactcgtcgc gcaagcgttc
     3661 gacgaggagt cactcgccga ccttccgggc acgatggcca tcgggcatac ccgctattcg
     3721 accgccggct ccaaccgcgc cgacaacgcc ggtccgatga tcactgacag cgatatcggg
     3781 cccgtcgccg tgtcgcacaa cgggaatctc accaacgcgc tggagctgcg tgaggaactc
     3841 gaggcggagg gtgaggagtt cgttaccacg acggactccg aggtgctcgc ccgggtcatt
     3901 gcccgagcgc cgggcctaac gatcgtcgac cgcatcagga acgccatgcg ccgtttggtc
     3961 ggggcgtttt cgcttgccgt gctcgccgag ggtcgcgtct tcgcggtgcg cgatccgtat
     4021 ggcgtgcgcc cgctgagcat cggcacgctc aatggccaca aggtcgtcgc ctcagagacc
     4081 tgcgcgatga tgacgatcgg cgccgaggtc gaccgcgaag tcgagcccgg tgagatcatc
     4141 gagattcgcg agggcgagat cctctcgcac ggcgctacgc cggccgaccg ggaagcactc
     4201 tgcctcttcg agatgatcta cttcgcgcgt cccgacagtg agatccacgg caagcgactg
     4261 catcaggtgc gccaggcgat ggggcgcgag ctctaccggg agcacccggt ggatgccgac
     4321 gtggtcatcg gcctgccgga ctcggccaca ccggcgacca tcggctatgc caaggagtcc
     4381 gggattccgt atgccgaggc gctgatcaag aaccgctaca tcggccgaac cttcatccaa
     4441 ccgaaccaga tgcttcgcga acgcggcgtg tcgctgaagt tcaacgtcgt tccggaagtc
     4501 gtcgagggca agcgcgtgat tctcgtcgat gacacgatcg tgcgcgggac caccagccgg
     4561 ccgatcgtca acctcattcg ggaagccggt gcgagagagg tccacatgcg cgtccatgct
     4621 ccgccgatca tgtggccctg ctacctcggc gtcgatctcg cccgcaagga agagctgatc
     4681 gctgcccggc tgtccgtgcc ggagatcggc caggagcttg gggtagactc cattgcatat
     4741 ctgtcgctgg atggcctgtt ccgggcgatc ggggagagcg gttcgggcta ctgcacgggc
     4801 tgcctcaccg gcaactaccc ggtgccggcg ttcacgagtc tggacaagct tgcgctggag
     4861 caccgaagtg tccagcgagc caaggtggga gtgagagcat gacgaccagc gccacggaca
     4921 ggcgcacggc gctctaccag caacatgtcg acctcggcgc gaggatggtg tcgttcgccg
     4981 ggtgggagat gccgatccag tatggcggca ttctcggcga gcacaaggcg gtgcgcaccg
     5041 ccgccggcct cttcgatctc ggccacatgg gtcaagtcaa cttcacgggc ccggatgcgc
     5101 tcgaattcct gcaatggaca accaccaacg atgtcagctc actcggaccg ggtgacgcgc
     5161 agtacgcgct cctgcccaac gagcagggcg gagtcgtcga cgacatcatt gtctaccggc
     5221 gacccgaggg cgaagcggga ttcctcgtcg tcgtcaacgc cgcaaatcgc gagcgagacg
     5281 tcgcctggtg gcaggagttg ctctcccgcc gtcccgacct cgatgtcacc gtccgcgacg
     5341 tctccgacga aaccggcatg atcgctatcc aggggccgcg agccgaggca atcaccgcga
     5401 aggtgacaga cgccgacctt cccagcatcg agaatttctc ttggcggcga gggacggttg
     5461 ccggcaagaa cgcgatgatt gcccgaaccg gctacaccgg tgaggacggc ttcgagttct
     5521 acgctgccaa ccagaacatt gctgccattt gggatgccct gctcaaggcg ggtgagggtg
     5581 acgggctcgt gccggtcggg ctcggcgcgc gagacacctt gagactcgaa gcgcgcatgc
     5641 cgctctacgg acaggagctc ggcgacgaca tcagcccgta cgaggcgggg ctcggctggg
     5701 cggtcaagac tgacgccaag gacttcgtcg gcaaggaacc gatagcccag gtcaaggctg
     5761 acaagccgaa acgacgaacg gttggatttg ccaccgtcgg ccgtacctcg gctccgcgga
     5821 gccactgccg cgtgctcgtc gacgacgaag aggtcgggta cgtaaccagc ggggcgtttt
     5881 cgcctacact tcagcagaac atcgggctcg ccctgatcaa tcgcgcctac gcgggagtcg
     5941 gaaagccgct ccaaatcgag gtccgcggca aggtggtcga cgctgagcag gtgaagacac
     6001 cgttctacaa gcgagccgaa tagccgagca gacatcaccg cgagctgcct gcccaaggtc
     6061 gggacgggta cagggcggtg tgcgatcttg tgaaaggaga gagcgatggc cgcacccgag
     6121 aacctgaagt acaccaagac tcacgagtgg gtgaaggttg accaagacac cgtcacactc
     6181 ggcatcaccg acgctgccca gacagagctg ggagacatca cctatctcga gctgcccgag
     6241 gctggcgacc agctctcctc cggcgaaccg ttcggggtcg tcgaatcggt gaaggccgcg
     6301 tcggatatct actcgcccat cgacggcgag gtggtcgagc gcaacgacga cgcagttgag
     6361 tcgccggaat tgatcaacca gtcgccgtac gagaaggcgt ggatgatcaa ggtcaaggtg
     6421 tcggacccat cccaactcga gagcctgctt tcaagcagtg attacgacgc cttcgcctca
     6481 gagtcgctgg gctagcacgc aaggacaacc gacggacatg agcttcaatc ctcacacccc
     6541 ggaggaccgc gagcggatgc ttgcgacgat cggggtggca tcgaccgacg acctgttcac
     6601 accggtgccc gaaacgatcc gatatcccca tctcgatctc ccgcccgcgc tgacggagct
     6661 ggaggccatt cgtcgcctcg agcacttggc cgagaagaac tttgtgccgg atgccgagga
     6721 tgtctacctc ggtgccggct cgtatcgaca tttcatcccc tcgacggtcg atcacctgat
     6781 ccagcgcggc gagttctaca ccgcctacac gccctatcag ccggaggtgg cccagggcac
     6841 gctgcaggtg atctacgagt tccagacgat ggtggcggag ctgctcggct tcgaggtcgc
     6901 caacgcctcc atgtacgacg gggcgaccgc gatggccgaa ggggcgttga ccgccgtctc
     6961 ggcgtcgcgc aagcgagaga agatcgtcgt gtcgccgacg gtccacccag cataccgaaa
     7021 ggtgctcgaa acctacgtgg aaggcacggc ggtcaccagc gagacgctgg aatcggccga
     7081 tcccttccgc tttgatgtct cggcgctcga gggcagactg gacgacagca ccgcctgtgt
     7141 cgtcatccag tacccgaact tcttcgggac gatcgaggat gtgcaggcga tcgccgacgc
     7201 cgtccacgag gccggcgcgc tgctggtcgt caacaccagt ccggtaccgc tggggctgct
     7261 gaagccaccc ggcgcactcg gcgccgatgt ggcgtctgcc gaggggcagc cgctgggcat
     7321 cccaccggga tacggcggac cgtatgtcgg cctgctggcg accggcaaga agcatgtccg
     7381 ccaaatgcca ggccgcctgg caggggtgac cgccgacgcg gagggcaagc gaggctacgt
     7441 gctgaccctg cagacccgcg agcagcacat ccgacgtgag aaggcgacga gcaacatctg
     7501 cacgaaccag ggtctcatgg cgaccgccgc gacgatctat atgtcgacgc tgggtccgga
     7561 gggattccgc gaggtcgcga cccgcagcta ccagaacgcg cactacctcg cggaccagat
     7621 cgtgaaacgg gagggttacg ccgtcgccaa caccggcgag ttcttccacg agttcgtggt
     7681 cgagtgtcct gagcccgccg cgtccgtcaa tcgacgattg ctggatcatg ggatcgtcgg
     7741 cggcctggac ctcggcacgg tggatgactc gctgaagaac ttcatgctga tttgcgcgac
     7801 ggagttgcac tctcggcagt cgattgaccg gtttgtcgag gcgatctagc ggactgctat
     7861 actgccggat gttctgaaga tcgctcgatg ttcgacgcga gtatccgtct cgcgcggcgg
     7921 catgcgctca atgctctgcc gccgcgccgg cagaccatgg tgacccagtg ccaaagccca
     7981 tcgtcgccct cgtcgggcgc cccaatgtag gcaaatcgac cctgttcaac cggctgatcg
     8041 gtgagcggcg cgccatcgtc cagaatgaac caggtaccac ccgggaccgc gtttacggct
     8101 caacggactg gaacggcgtc gacttcacga tcatcgacac cggaggtcta caggaggact
     8161 cggacctccc gagagacgag accgaggtgc gacggctgat cgcgcggcaa acgcgcaacc
     8221 aggcgaccat ggcgatcagc gaggcggatc tcatcgtgat gctcgtcgac gcccgcaccg
     8281 gcccgacccc gcaggaccag gagatcgccg atctgctgcg gcgatccgac aagccagtgg
     8341 tgctcggcgc gaacaaggcc gagcgcaacg atctccgatt cgaggcgctg gagttctaca
     8401 acctcggact cggcgaaccg attcccatct cggcctacca cggcaacggc actggtgacc
     8461 tgctcgaccg cgtcgtcgag gaactcccga acgcggagga gctggaggaa gacgaggtcg
     8521 aaggaccgaa gatcgcgatc gtcgggcggc cgaacgtcgg caagagccga ttgctcaacg
     8581 cgctactcgg cgaggaacgc gcgatcgtct cggaggagcc gggcaccacg cgcgattcgc
     8641 tcgacaccat aatcgagtgg gagggcgagc agctcaccct gatcgacacc gccggcatcc
     8701 gccgccgtgg gcgggtggag gaggggatcg agcgcttcag cgtgatgcgg tcgatgcgcg
     8761 ccatcgaccg ggccgacgtc gtgctgctcg tgatcgacgc tactgaggac ttcactgccc
     8821 aggatctgca catcgccggc tacatcgagg agcagaagaa gggcatcgtc gtcgtcgtca
     8881 acaagtggga cctcgtcgag aaggactcga ggtccatgga cgagtaccgc gagcgagcgc
     8941 gccgagagct cgatttcatg ccgtacgcgc cgctggtctt catctccgcg aagaccggcc
     9001 aacgggtggg caaggtactc gaaatggcgc tcggcgtcat cgaggagcgc aaccggcgcg
     9061 tcaacactgc cgcactcaac cgaatgctga aggatgcggt taccaagcac ccgccgccat
     9121 cgaaaccggg caagtgggtc agattcttct acgccaccca ggccgacact gccccgccga
     9181 cgttcgtctt cttctgcaac gatcccaagg cgatccattt ctcctatcgc cgctatctcg
     9241 agaatgaact gcgggaggcc ttcgacttca gcggaacccc gctgcgcatc tccttccgga
     9301 gccgccacga gccaagctga ccatgcccct gatctggtcc attctcggaa ttgccgctgc
     9361 ctacctctcg gggagcatcc cgtggggagt ggtagtcggg aagctgatca agggcatcga
     9421 tattcgaaag atcggctccg gcaacacggg cgccaccaac gccatgcgcg ccttcggctg
     9481 gcggatcgca ctcctcgtag cggtgctcga cgtgctcaag gggctggtac cggtgctggt
     9541 ggcgcgctgg ctcgacgcgc cgacctggac ggtggccctg atggccgtcg cggcgactgc
     9601 aggccattgc tggtcgccgt tcatgaagtt ccggggcggc aagggcatgg cgaccggcgg
     9661 gggcgcgaca atcgggctgc tgccatggct gctgaccatg ttgcccttga tgatcatcat
     9721 cgtgctgatc acacgctacg tgtcgctcgc gtcgatcatc gcgatgatca ccgggaccac
     9781 catcgcctac attttcgccg cgaccacccc cttctcatgg gtctgggcga ccgcagtcct
     9841 cggaatctcg gcgatcgttg tcggcaagca tcacggcaac atcatgcgcc tgctcacggg
     9901 caccgagcgc cgcttcggtg tccccgagtc ctcctcataa tcctcttgcg tcgcgctagc
     9961 tcccgtagcc ctcgggatgc cgctgcaacc actcccacgc ggacccgatc atctcgtcaa
    10021 gcgttgaatg cgccggtctc caatcgagaa cctctctcgc ccgggtcgag tcggcgatca
    10081 gggtcgccgg gtcgccctcg cggcgcgccc cgtaggtgac cggcagttcc cggccggtca
    10141 cccactgcac ggcatcgacg atttgctgca cggagaagcc gttggccgtg ccgaggttca
    10201 tcgcgccgag cggctcgtcc agcctgtcga gcgcggcaat atgtgcgtcg acgaggtcga
    10261 tcacgtgcac gtagtcccgg acggccgtgc catcagctgt gtcgtagtca gtgccataca
    10321 cgacgaaacc gtcgcgctgg ccgaggagcg cctggagcgc gaccggaatg acgtgcgtct
    10381 cgggattgtg atcctcgcct cgctgctctg acgcgcccgc cacgttgaag tagcggaaga
    10441 tagcgtggtt cagaccgtgc tgccgcgcga acgcctccag catttgctcc accatccatt
    10501 tggactgccc gtaggggttg atcggcagct tcggcgagtt ctcgggaatc ggcaccgttt
    10561 ccggctcgcc gtagactgcc gccgtcgacg agaagacgaa tcgctggatc cctgcgtcgc
    10621 gggccgcctc aagcatatgg agaccgcccg aggtgttggt ggcgaagtaa gcgcctggct
    10681 ccttgacgct ttcggggacg agctggagcg cagcaaagtg catgatcgcc tccgcaccca
    10741 gccgctgcac cgcatcccgg gtcgcgacgg cgtcacggat gtcgcattcg acaatagcgg
    10801 cgtcaggatg cacggcctgc cggtgaccgc gcacgaagct gtcgagcacc gtcacccggt
    10861 ctccgcgcgc caccaaacgc tccacggcga tgctgccgat gtagccacct ccaccagtca
    10921 caagaacgtg catatttggc tccgtacccg ctagagaccg cgctgtccgg cccgccagcc
    10981 cgagccggaa ggacacaggc accatggtac acttccgcgg aacgaccggt ctcttcccac
    11041 acggcgaccg ggccccgcaa cgcccccttt ccccaatagg tttctgagga gtgagtgcat
    11101 gaccgcgtcg gagaagctcg cccagtatga ccccgaggtg gctgcggcga ttcgccacga
    11161 ggagcagcgt cagcgcgagg gaatcgagct gatcgcatcc gagaactatg tcagcgaggc
    11221 ggtactgacc gcggtcggct ccgtgctgac caacaaatat gccgagggtt tgccgggaaa
    11281 gcggtactac ggcgggtgcg agtacgtcga tatcgccgag gagctcgcga tcgatcgggc
    11341 caagacgctg ttcggggcgg aacacgcgaa cgtccagcca cattcgggag caagcgccaa
    11401 cctggcagcg ttcttctcac tcatggatcc aggtgacacc atcctgggca tgaacttgtc
    11461 cgaaggcggt catctcacac acggctcgcc agtgaatttt tccggccggt tcttcaacgt
    11521 ccacgcatat ggcgtggatc accagacggg aaccatcgac tacgacgccg tgctggaaaa
    11581 ggccaaggag gtccggccga ggggcatcat ctgcggagcc agcgcctaca gccgcatcat
    11641 cgacttcaag cggttccgcg agattgccga tgaggtcggc gcatatctct ttgcagatgt
    11701 cgcacacatc gccgggctca tcgtcgccgg tgagcatccc acatctatca gccacgcaca
    11761 catcaccacg accacgaccc acaagacgct gcgcggtcca cgcgctgggc tcatcctgac
    11821 cagcgaggag catgccaaga cggtcgacaa gaccgtcttc ccgggcatgc agggtggacc
    11881 gctgatgcac gtcattgccg gaaaggcggt ggccttccag gaggcgctcc agccgtcgtt
    11941 caagacctac gcgaagcaga tcatcgccaa tgcgcgagcg atggcctcgg ccctgcagga
    12001 gcgcggcctc cacatcatct ccgatggcac ggacaaccat ctgttcctcg tcgacgtcgg
    12061 ggagaaggga atcagcggac gcaaggccga gcgggtgctg gacagcgtcg gcatctccgc
    12121 gaaccgcaac accattcctg gggagactcg accgccgaca caggcgagtg gcctgcggat
    12181 cggcacgccg gcgatgacca cgcgaggctt cagggaacag gagtcggtgc aagtcgcgga
    12241 catgatcgca cgggtgatcg aagcgccgga ggacgagcag gtgctcgata ccatccgcaa
    12301 tgaagtccgt gagatgacgg ccacctatcc ggtgcccgga accacgccat cggcatccaa
    12361 gtaggtgacg atgcagggaa cgcgaaaggg cggtcgggta taaccccgac cgcccttatt
    12421 gccccttgat ggcctcgcta ttgcagaagc agctcaaacc gcagccgctc gtcgtcgctg
    12481 cgatcgcgcc ggtcgatgaa ggcccagacc accgacagga gatgcacgag cgatcggccg
    12541 agcgccgcac cgagaacgaa ccccactgtg gcggccccga tgctggcgaa cacgagcgtc
    12601 aggacgctgt tgtcggattt cattgagcag tgtctcctcc agcg
//
LOCUS       DASYWL010000022         2284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_470195, whole genome shotgun sequence.
ACCESSION   DASYWL010000022 DASYWL010000000
VERSION     DASYWL010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470195"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..340)
                     /locus_tag="VGR22_00830"
     CDS             complement(113..340)
                     /locus_tag="VGR22_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127146.1"
                     /translation="MRNHPYPDDPLRQRGVKARENLVNALRECGELADAVETFDGVEL
                     LEVLDYLDSLRFVMAESGQLLAGVVRGEMSG"
     gene            complement(366..2012)
                     /locus_tag="VGR22_00835"
     CDS             complement(366..2012)
                     /locus_tag="VGR22_00835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2127147.1"
                     /translation="MPTPAPNPPRHARPNVNPWAVLAVLCTSIFMLLLDTTIVNNAQR
                     KIQIGLDAELSQIQWILDSYILVYAVLLLSFGRMGDIFGRKRFFVAGIVVFTLASGLC
                     GLSPLIGDWLGVGGATTLIAARALQGFGGAMMMPQTLSIITQVFPPEKRGSAFGIWGS
                     VVALGAVSGPVIGGAITTYYSWEWIFLINLPIGALGVVASARILPETRDPNASRTLDW
                     GGVLLSGTAIFMLVFALIEGNRIGWCSPMIVALLAGATIIFAIFFRWEVRHPEPMVRL
                     ELFRIHNFAVSNAILMLVAFGVFGIFFPFTLFLQAGVGYTPLEAGLVTVPMSIMHGTV
                     APIAGRLTDRYGARPFIIGGLAMVTIGIALIAWQTTTETTWQRLMIPTSLTGAGMGTV
                     MSPLTSAAMHDVPQGIAGSASGIFNTMRSIGQILGIAVLGTLLQTRTATEADTRLDRL
                     DIAPALQQDLVDKAEASQFEEIVARLDTLPDSIPLLLPPIQHAFADAAQFTFFTNASI
                     AAVTLLIAFQIRSPQRAEKRAAQPARPRERPQPVRASGSK"
BASE COUNT          437 a          676 c          797 g          374 t
ORIGIN      
        1 ggtgccggga ttcgacgcat ggaaccagcg gcgcgagaga tttctccact tcgtacgctt
       61 tgcgtgcttc ggtcgaaatg acattccccg ttgtacgtga tccgccggtc ccttacccac
      121 tcatctcgcc tcgcacgacg ccggcgagca gctggccgga ttccgccatc acgaatcgga
      181 ggctgtcgag gtagtccagc acctccagca gctcgacacc gtcgaacgtc tcgactgcat
      241 cggccagctc gccgcactcg cgcagcgcgt tgacgaggtt ctcacgtgcc ttgacgccgc
      301 gctgacggag cggatcgtcg gggtagggat ggttccgcat gctggtgctc tcctcagggg
      361 ccagcctatt tgctgccgga cgcacggacc ggctggggtc gctctcgcgg tcgtgccggc
      421 tgcgccgctc gcttttccgc ccgctgtggc gagcggatct gaaaggcgat caggagcgtg
      481 acggccgcga tagacgcatt cgtgaaaaag gtgaactgcg cggcgtcggc gaaggcgtgc
      541 tggatgggag gcagcagcag cgggatggag tccggcaggg tatccaaccg ggcgacgatc
      601 tcctcgaact ggctcgcctc cgccttgtcg acgagatcct gctgcagggc cggggcgatg
      661 tcgagacggt cgaggcgggt atcggcctcg gtcgccgtgc gagtctgcag cagcgttccc
      721 agcaccgcga taccgaggat ctgcccgatg ctgcgcatgg tattgaagat gccgctggcg
      781 ctgccggcga taccctgtgg gacgtcgtgc atcgcggccg aggttagcgg tgacatcacg
      841 gtgcccatgc cggcgcccgt gaggctggtc ggaatcatca ggcgctgcca ggtcgtctca
      901 gtggtggttt gccaggcaat gagcgcgatc ccgatcgtca ccatggcgag cccgccgatg
      961 atgaaaggac gcgcgccgta gcggtcggtg agccgccccg cgatcggggc caccgtaccg
     1021 tgcatgatcg acatcggcac ggtgacgagc ccggcctcga gcggcgtgta gccgactcca
     1081 gcctgcagaa agagggtgaa cgggaagaag atgccgaaga caccgaaggc gacgagcatg
     1141 aggatggcgt tggagacggc gaagttgtgg atgcggaaga gctccagccg caccatcggc
     1201 tccggatgtc gcacctccca tcggaagaag atggcgaaga tgatcgtcgc gccagcgagc
     1261 agcgcgacga tcatcgggct gcaccagccg atgcggtttc cttcgatcag cgcgaagacg
     1321 agcatgaaga tcgccgtgcc ggagagcagc acgccgcccc agtcgagggt gcggctggcg
     1381 ttggggtctc gggtttccgg caggatgcgc gcgctggcga ccacaccgag ggccccgatc
     1441 ggcaggttga tgaggaagat ccactcccaa gagtagtagg tggtgatcgc gccgccgatc
     1501 accggcccgg aaaccgcacc gagtgcgacc acactgcccc agatgccgaa ggcgctgcca
     1561 cgcttctccg gtggaaagac ctgcgtgatg atcgagagcg tctgcggcat catcatcgcc
     1621 ccgccgaagc cctgcagcgc ccgggcggcg atgagtgtgg tcgctccgcc gacgccgagc
     1681 cagtcgccga tcagtggcga gagaccgcag agacccgagg cgagcgtgaa gacgacgata
     1741 ccggcgacga agaacctctt gcggccgaag atgtcaccca tgcgaccgaa cgagagcagc
     1801 agcaccgcgt agacaaggat gtaggaatcg aggatccact gaatctggga gagctcggcg
     1861 tcgagtccga tctgaatctt gcgctgcgca ttgttcacga tggtagtgtc gagcagcagc
     1921 atgaagatcg aggtgcagag caccgccagc accgcccacg ggttgacgtt cggacgcgcg
     1981 tgtcgaggtg ggttgggggc gggtgtgggc atggacagcg tcctcaagtg cggccggcaa
     2041 cccctccatg atgggtgagc gagcagcggg atgcaacgtg tacatggaag cgggagcgcg
     2101 atatcgggct gcaattcacc cgattccacc gagtacgtac actgatcagc ggcgaaaatt
     2161 acgtaatttt accgatgaac cgaggcgggt actggtcgaa aatggagtga ctgaaggacg
     2221 agccacggtg gtggcgatat ccagaggtat cggaagaaga gcctgtgtgg tgccgggtct
     2281 cggt
//
LOCUS       DASYWL010000023        24951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_320011, whole genome shotgun sequence.
ACCESSION   DASYWL010000023 DASYWL010000000
VERSION     DASYWL010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 24951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24951
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320011"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..488)
                     /locus_tag="VGR22_00840"
     CDS             complement(105..488)
                     /locus_tag="VGR22_00840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEV2127148.1"
                     /translation="MSPAGAQVLEARGRKSGKLQTVPVNPIEVGGQRYLVAPRGETQW
                     VRNVRAAGEGTLRVGGRSERIAGREIPDADKPPVLRAYLDKYYRQVGAQFGVPKDVTL
                     DQLADIAGNHPVFRIDRPDAGSRHK"
     gene            complement(509..1000)
                     /locus_tag="VGR22_00845"
     CDS             complement(509..1000)
                     /locus_tag="VGR22_00845"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEV2127149.1"
                     /translation="MACYQKPNWFTAKVANPAVQFFIKRFGMSPAGAQVLEVRGRKSG
                     KTHTVPVNPIEVEGHRYLVAPRGETQWVRNFRVAGEGVLRVGKRAEHIVGREIPDVDK
                     PPILKAYLDRYHWEVGSQFGVPKDATLAQLADIAPNHPVFVIHAREFAKGDDGLGSSP
                     YDA"
     gene            complement(1243..2430)
                     /locus_tag="VGR22_00850"
     CDS             complement(1243..2430)
                     /locus_tag="VGR22_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEV2127150.1"
                     /translation="MPFVPQKVFKDRAKVIREWLGKHELKALIVTTPDNFYMVSGFHL
                     DVAPWERPVAAVIPAEGEPFMIMNELSTNHLKFAEERKSLFITDYTIYAEHPRTTNRT
                     WTVLQFGDLVAHRLGERGITKGRVGVEGSGPAALRTAAPKLVFEDVSQYLVSLREVKY
                     PEELEILRECAKLTDWAQDKYMELVKPGLNVKAFDLHIGSLAIEEAARRFPKDRFEPR
                     MMSLSGPASAAPHGTGADVGETFEKGHGVVNIIVFRLSGLVVESERTLFLGKPNDTQR
                     RAFEAATEANEAAAAQMVAGNPVSSADAAAQQVIEKAGFGEHINHRTGHGIGIAGHEF
                     PDDMPYLHRPFKEREIFSSEPGIYIWGVGGFRHDDTVVVGKNKPEVLTRRSRKLEDQV
                     VRV"
     gene            complement(2442..3317)
                     /locus_tag="VGR22_00855"
     CDS             complement(2442..3317)
                     /locus_tag="VGR22_00855"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2127151.1"
                     /translation="MVATAQTLDGARTRQNRFVRALLRDPWAMMAILFLVVVTVVALV
                     SPLIVPDDVNSPDFGARLLPPLSDGHIAGTDQLGRDVWDRMLLGARISLFVGFVTVLC
                     AALVGVPIGMLAGYFGGWIDTILMRLVDVQLSFPFILLALTINAILGLGLRNIIITLI
                     IAGWIEYARVARGETLVVREREFVQAAHLNGAGSGRIFLRHVFPNIITPLIVIGTLQV
                     ARFIIAEASISFLGFGVQPPTPAWGSMVSEGLDYIFSAWWLITLPGLALALVALAVNI
                     TGDWLRDTLDPRITE"
     gene            complement(3317..4231)
                     /locus_tag="VGR22_00860"
     CDS             complement(3317..4231)
                     /locus_tag="VGR22_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2127152.1"
                     /translation="MKAYVFGRLVSGAIVVFLALTAVFFMLRLSGDPVLLFAPMDTAR
                     TDLEQIRERMGFDDPLAVQYVRFMTDAVQGDFGDSTRERRPATEIVLERLPATLQLGG
                     VALLISIVVGIPLGIVSATRHGSGWDTLARIIAVAGQAVPGFWLGLLLILLFSVRLGW
                     LPTSGRGGLEHIILPAITLAALSTARYARLTRSTMLDVLGQDYIRTAKAKGLGGQVIL
                     WRHALKNASISLITMTGLEIGRLIEGAVIVEQVFAWPGMGRVTVQALLNRDFAVVMAA
                     VVLFAIMYTLANLLADLAYGWANPQVRL"
     gene            complement(4361..5938)
                     /locus_tag="VGR22_00865"
     CDS             complement(4361..5938)
                     /locus_tag="VGR22_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2127153.1"
                     /translation="MTKRSMELNRRNFARMAGATPFAAALGTPLALSYSAQVAAAQDD
                     MLEQVVVALGADPLTMMPNSIVDWTTDIQLAHMFDRAMNYDPEQNYEVGPWLCELTNI
                     DDLTWELTLVSPDITFHSGNPLTANDFKYAIEWAQDPANESHYLERWEQFEEVTIVDN
                     QTFRIKTTDPFPLIAQRMVELYPVDSALIEEIGIEEYIENPSGTGPFTFQEWVRGEYL
                     RLERNPDYWHNEVQVQEVEFRFMPEFSSRLAALLAGEVQIVKDIPVDSIPNVEDSENA
                     RVEEIPSSRVNYVALVNNREDSVFTDVRLRRAVNHGVDVQAIIDGVFQGHATRMAGAL
                     SNLNPEVNAEIEPYPYDPELARELIREAGYEPGELTVTMDAPQGRYPMDTDAAQAIAA
                     SLTDTGINVEVQYNEWGTHLDKIVNRQTGDMFYLGWGPALDAYGTLVFLFIGDSTYSS
                     YSNDEVTPLIEEAMVTVDEERRQELWNEVQQIVYDDAGWLFLWQQHDIYGVTNDVAWE
                     PRPDEFMWMGQAEPREA"
     gene            6249..7190
                     /locus_tag="VGR22_00870"
     CDS             6249..7190
                     /locus_tag="VGR22_00870"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEV2127154.1"
                     /translation="MSHTASPSDEIRTVTVIGAGFLGRQIAALAAASRREVRLYDANP
                     YAAPAARSLLRDYLASPVADGLLDWDLDEVLARIHPVASLEEAMRGTDLMIEAVREDL
                     ETKREVFGAASAANPDAYLATNSSSLMSAQLRDVVSQTDKLVNLHFFGEFWNRSMVEL
                     MGCGETSEETIITLHRFGQSLGLYCAVVRGQSKGFIINRVWRAVKREALRVVDEGHAD
                     PEDVDRLWALFWGTQQGPFGAMDRVGLDVVADIEDSYIAVATDPTDRPSRMLHELVEA
                     GRLGIKTGEGFYEYPNPAYRAPGWPRTSPSGDDSAGR"
     gene            7336..9108
                     /locus_tag="VGR22_00875"
     CDS             7336..9108
                     /locus_tag="VGR22_00875"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV2127155.1"
                     /translation="MSRQFRFVPHHSRRRFLQTSSALGLSVVMTSRSDLAIAQSSTPE
                     AESEQLWYEDTQGPPEPGGTVRFLLYEDPNTLNPLLGSTSIAVQVVTAVLEGLAENAP
                     DGTFVPLLAAELPTPENGGVSEDLLTVTWKLKEGVTWSDGTPFTSDDVLFTWEAARDA
                     TNGSALASDYELISSVETPDELTVVVNYSQFNAGYVDQFPWILPRHATGPVEEMRDWT
                     FNRAPIGTGPFKFDEWSSGEFVQLSANENYREEDKPYLDGLLFLVVPDESVRTAMMIE
                     ENAEIMLWSGQEADEQITAAGVGASRTAPGIWVVEMRFNLSQPFDDDPGPEPPHPILG
                     DPRVREAITLGVDRDRITGELVTGTVFELDSPLEVGWMDCQVDPFVYDPERARELLEE
                     VGWRDEDGDGIREAHGVETVEDGTRLSLMMNGYTGFDTLDLIELAVQEDLANIGMEIN
                     IENQEFAVIFGTWEDGSPRMLGDYDILIYDAGLFAEPGADIANRYHPDRVPSADNPGG
                     ENIYRWVREDVGEWIEAANSSPDLEVRRENFCNVAEALREDIVTFPILQFSEGSAYSN
                     RLHGFTVSTWEWATWDAENWWLEQ"
     gene            9200..10189
                     /locus_tag="VGR22_00880"
     CDS             9200..10189
                     /locus_tag="VGR22_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2127156.1"
                     /translation="MGPYIIRRVLWAIPLLLFVSVIVYGLLELSPGGPTSMYLRRGTG
                     MSAEDLARMEESLGLNDPFYEKYGRWLWRVLQGDFGMATTSSRPVSDEILERLPNTLY
                     LMTIAWAVSLLIAIPIGIVSAVRQYSKFDHFVTAMTFIGQSIPSFFLGLILLLIFYMR
                     LDNPFSGRPLLPSGGVETLGADLSGWDMIWDRIKHLILPVVMLSATWVAWYSRFLRAS
                     MLETIHLDYIRTARAKGVAERAVILRHAFRNAALPLVTLMALDLPLLFAGALYAEVIF
                     SWPGMGRLFYQAAERRDYGLLMAIVMITSLLIIVGNLLADVAYRFLDPRIKLR"
     gene            10205..11152
                     /locus_tag="VGR22_00885"
     CDS             10205..11152
                     /locus_tag="VGR22_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2127157.1"
                     /translation="MAGSPEASASTTTQNRPLQSEDTMARTESRSMLRIYVRRFLAHR
                     LAAAGLVFLVIVTLSAVLAPAITQDPNALSASDRKAAPSADHWFGTDEAGRDILARTI
                     HGGRISLAVGITATALALFIGTLIGAVAGYFGGAVDAALMRLTDAFLCFPQLFVLIVF
                     GTLMRTTELRVLQGNMTPIAIIIGLLSWMGLARIVRATFLSLREQDYVEAARSVGAGN
                     ARIMIRHVLPGAVGPIVVQATLLVAVTIITESGLSYLGFGVQPPTSSWGNLLEGAKPF
                     LQTLPVYAIAPGMMIFLTVMSINFVGDGLRDALDPHSQH"
     gene            11240..12622
                     /locus_tag="VGR22_00890"
     CDS             11240..12622
                     /locus_tag="VGR22_00890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2127158.1"
                     /translation="MKDYRRYSFWLETCGDDLTPRPPLDSSIDVDIAILGGGFTGLWT
                     AYYLLSKEPSLNVAIVEAEIAGFGASGRNGGWCFAGFPVSPVTLLQSHGYDAARLVSL
                     EMYKAVDEVGRVTREEGIDAHYAKGGELEIARAAYDLPKLQKMYDEFRAIGLEDHYRL
                     LDAEETTERINVARAAGSFHNREGAVIQPARLARGLARAVERRGGTIYEQTRVIDFTG
                     APNPSLVTAWGTVRAKTIVLAGEAYLSALPGMDRSIIPMTSHIVVTEPLNDELWEQIR
                     WWDRDTLGGFGVNGGYIQHTADGRIAFGPYRGNYPFNSRISDDLDRDEKIFAHARKST
                     LDWFPMLQGVKFTHAWGGVFGIPRDRMPTMNYDPRTGIAAAYGYTGDGVATANLSGRV
                     LTDLITETDSPLQMLPMTQGSQRRWEPEPLRWMGVTFVRKDRQRVLKKIEHKGKHPEK
                     PSLSQRLYSY"
     gene            complement(12657..13232)
                     /locus_tag="VGR22_00895"
     CDS             complement(12657..13232)
                     /locus_tag="VGR22_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
                     /protein_id="HEV2127159.1"
                     /translation="MTTIRTADREIDVRIVYYGPGLSGKTTNLVNIYQRMPGDLRGPM
                     RSIDTDQERTLFFDFLPVEPVRVGGWSLRFQIYSVPGQESYARTRRAILTGVDGVAFI
                     ADASPDRLRANQESLQELADHLERQGRTLTEIPVVFQINKMDQAAGRSTIEIASQIGA
                     GEWPVVEAVAVRGVGVRETLERAVRDIARAL"
     gene            complement(13423..14517)
                     /gene="purM"
                     /locus_tag="VGR22_00900"
     CDS             complement(13423..14517)
                     /gene="purM"
                     /locus_tag="VGR22_00900"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEV2127160.1"
                     /translation="MNDTPCTGPDIGTETGVTYRDAGVDIDAKMSAIEEVKARAAQTL
                     GDTAGPIGHFGGTYRLPGAPGQILVASADGVGTKLRLAFALGGDAHARMGEDIVNHCA
                     NDILALGARPLFFLDYFATGRLAPEVVTGVVGGIADACAVNGIALLGGETAEMPGMYA
                     DGEYDLAGFTVGAVQEHAIVDGSAIEPDDLVIGLPSTGLHTNGYSLARRIIGMNGDPE
                     HDREVLAKQLPGASKDETLGEALLAPHRSYVREVTPLLEDGLVRGMAHVTGGGLLDNL
                     PRTLPEDCIARLDPTVWETPEILDFLVNRGQISVAERYRVFNMGVGFVLVVSPDHVDE
                     MLARLPEGRPIGMIISRVTSDESAVQGLSC"
     gene            complement(14583..15977)
                     /gene="hflX"
                     /locus_tag="VGR22_00905"
     CDS             complement(14583..15977)
                     /gene="hflX"
                     /locus_tag="VGR22_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEV2127161.1"
                     /translation="MVTPFDADTALQIGDTERALLVAVETQGQAWAAQDSLEELARLA
                     ETVDVEVAGSVSQKLKAPHPAAYVGKGKVEELRDLRESLDYTLVMVDGELSPAQQRNL
                     ERALEVKIIDRTALILDVFARRAQTHEGRLQVELAQLEYRLPRLTRMWTHLSRQGVGG
                     VGLRGPGETQLEADRREAQKRISFIKDQLAQVHQHRERYRKRRRQTPISVVSLVGYTN
                     AGKSTLLNTITGAEVLAEDKLFATLDPTTRRIQLPSGREVLLTDTVGFINNLPTQLIA
                     AFRATLEEISEAAVLVHVVDGTHPSAAEQVETVNRTLEDLAVDDRPVVFALNKTDRLS
                     PDERERLPQVASSLGVPDDAVGISARTGLGLDRLFEAVEAALEEEAGFVAVSGTIPFD
                     RTELVDLFHKLGRVEDHAYDEQGTTMTGQLPARELGRFEPWLVPDDIAEAGPDTEPGA
                     EGKDEEPRRDRVAS"
     gene            complement(16051..17847)
                     /locus_tag="VGR22_00910"
     CDS             complement(16051..17847)
                     /locus_tag="VGR22_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127162.1"
                     /translation="MWRALLIILLLFPGIAVTPAAAQDGDVAPPVDLATMPLTPDQLP
                     DDRYQFARGAYLTRDDARYLIEQRYAIDAGVVDTVITGARWRQGYTASFVLLSDRASD
                     PIVAVTTTIHALENSDAATVLETLLIGAPPEGAEERGPALGEAVTWRVVSSQDDTRRI
                     LLRIGRFVVEVEMAYRQVPPDPVEHAEVVADTLDRIERVLRFGGPGLSRQMMLVDDDR
                     MVPVAVSLESPLVHTWYRMLDEEVVLASGELAAPSLPEGATDIAIARQTAEVASQSWV
                     TIGVVVARFETESGAAGFAESDIIRDPLDIFAAGESEVSIESVEPRLVVTAISGESNV
                     GGRWSGYRVTLARGDLAAQMTIRSTGPTLVDRDAVEAWAVLQWACLAEDTYGPVPLAD
                     LLQVGRNATPAAIGDVPGVYESPVAPWSVIYNPTVWDAQHTFAEGGYDYLYLRADRID
                     ATFETIVDHRGDPEACVLDELELLREDEEHARIDVGSDVEGEEPGGLENGHAWILYTV
                     EPLDEERAGEEYVIRIDCYTVVEGSTSLVVGTRAPRDVWAEVAPMGEQLRAGIEIDGA
                     PAGQETGDRLAAALSWRTGVMIDRRLWVGLAA"
     gene            complement(17928..18743)
                     /gene="lgt"
                     /locus_tag="VGR22_00915"
     CDS             complement(17928..18743)
                     /gene="lgt"
                     /locus_tag="VGR22_00915"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEV2127163.1"
                     /translation="MPVLDDPTAFSLGALDIRWYALFILTGIFAAIGLGSWLAERRGL
                     DGEFAFDLAPVVVISALVGARLYYIILRWDYYLDHPVEAINVRLGGLTIHGGIIAGVL
                     AVWWYTRRRNQPFLTWTDIIAPSLALAQAIGRWGNWANQEAFGTPTDVPWAVEIAPEN
                     RPRAYAEFETFHPTFLYESIFNLLNAIFLSWLVLQVPKRASLRAGDVTGIYLIIYGVV
                     RLFIERIRTDSLYIGPWPAAYWLSLALIFAGTGLMVGTRWWASRSTSTVEAAP"
     gene            complement(18945..19898)
                     /locus_tag="VGR22_00920"
     CDS             complement(18945..19898)
                     /locus_tag="VGR22_00920"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEV2127164.1"
                     /translation="MVDPNRPVVSIGECLVDLIAENQLDLLHADRFAVREGGAPANVA
                     VALARLGVPVMLCAVVGRDPFGYRLRDILQNEGVDTRALRMTSEAATSLALAWTNARG
                     DGEFQLLRLADRLLSSNDAEAARPWEASALVAGSVVLSDSESRRGAVAALDLAVSNLV
                     PVVFDLNLRPTLWRSMEQVRLAVEQFLHSARVIKVSMDDARGVWGIETPQEALDRLAS
                     YSARTTIVTDGGRGVWYRNERKTARSIPVFSVDVVEPTGAGDAFTAAITSRLLASSWM
                     PVTDEDVRFAMAAGALATTKPGAWPGLPTARAVEVFLRDRE"
     gene            complement(19904..22186)
                     /locus_tag="VGR22_00925"
     CDS             complement(19904..22186)
                     /locus_tag="VGR22_00925"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEV2127165.1"
                     /translation="MSIVAQRPLLEELARLDPPDSAVSGLDLRNLEQSDPRVLDALNR
                     LAGAVDANTIIGDVAMIARGVMLAARAHDGFNRKSGEPYFVHPLEVAIFLCHMHLDAE
                     TVVGGLLHDAIEDTDVTSEEIAERFDERVSRLVDGVTKLSRLKWAPGETERSVKEQQA
                     ESLRKMFLAMVDDIGVVLIKLADRLHNMRTLEAMPREKQIRIAQQTMEIYAPLANRLG
                     IWQVKSELEDLAFRYLQPEDYARITKELKRHGAGTEEVIGPVLRKLEDLIAAAGIRAE
                     MSARKKHIYSIYRKMNRKRRAFDEIYDVIGVRIIVDTVQDCYSVLGIVHGRWHPVPGE
                     IDDYIATPKESMYQSLHTAVIGPKGQPVEIQIRTKEMHQLAEYGVAAHWRYKEGGQAD
                     QRLEAKVAWLRQLMDWRDEVADAEEFVESLKSDVFQDMVYCFTPAGDIIELPNGATPV
                     DFAYRIHTQVGHHAVSATVNGQMVPLNYKLQNGQVVKIKTSKTVSGPRRDWLQAGDEY
                     VKTASAREKIRQWFRRQQRDENIAQGKQILERELKRLGVEKKLEDVLELFPRYSKLDD
                     FLAAIGYGGVSAQQIVSRLDETPKDVLTATTHVKAPSSPARVEVLGVGNLLTTQANCC
                     RPVPGDPIVGYISRGRGIIYHHRDCTNVTNLPDPDRLVPVSWGKESQATYPVSVRVSA
                     LDRVGLLKDMSIMLAEDHVNILSVLTQTHDDRTVTLLLTIEVEGIRHLSRVMHRLEAV
                     RDVLEVQRDNSATARQAWAS"
     gene            22570..23820
                     /gene="glp"
                     /locus_tag="VGR22_00930"
     CDS             22570..23820
                     /gene="glp"
                     /locus_tag="VGR22_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEV2127166.1"
                     /translation="MTDHQALQELDRMRPPDEARRVIEAHLPTLPTEHVPLTEALGRV
                     LVEDIAAPFDHPPFPASTMDGFAVLAADASPWREVIGEQPAGTMIAEEVHDGQAVRIM
                     TGAPIPTGADAVVPVEETSPAEDHVVIHAEDVTPGTNIRPVGSDLKRGEVVLRTGTRL
                     HPAHIGLIASVGIAQVPVRRRPRVSILATGDEVMEPGSELKPGQIYDANRFSLSAAVL
                     DAGGEITWSGLAPDRRDQLEALLRERMETDDIILTSGGISMGELDLIKAILFDAPDVT
                     VHFRRLFMKPGKPLNFATRGDALIFGLPGNPVSSLVTFELFLRPAMAIMSGDSKPNRP
                     RVPVTLSSPAAPSDRIEYQRARVTVTPEGKLVGASTGPQQSSRLASFVGANALLVIPP
                     RETSYEPGEMVEALLLAPPDSESR"
     gene            24062..24823
                     /locus_tag="VGR22_00935"
     CDS             24062..24823
                     /locus_tag="VGR22_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127167.1"
                     /translation="MPKSATLSAPAWSETDIAATPVTAEPRDGRPITIDDLYAFRMVG
                     DPQVSPDRSSILTTLLTIDHDTDEYRAATWRMDADDGNTRQLTSGEWRDGSPRWSPDG
                     RWIAFSSKRGNDDAKTQLWVMPTDGGEPIRVTSFDNGVLEHDWAPDSQRLVVVSLVEM
                     KPEHAETEADVRVITSAHQVQRAWLRRPPLPATLRRRPYRAGRRTAAADRGLVPAPLP
                     GVVSGRARDRVRHQPQPRLGHLPHLGHLDRPGAWR"
BASE COUNT         4306 a         8467 c         7898 g         4280 t
ORIGIN      
        1 cgcttgacgt gcgcggcgtg gacgaggcga cgtaactgag atgctccatt ggggtcgacc
       61 ttgtgtcggc ccaaaactct ggggggaagg ccctcaccta cgaatcactt gtgccgcgat
      121 cctgcatcgg gcctgtctat cctgaagacc gggtgattgc cggcgatgtc ggcgagctga
      181 tccagcgtca catccttcgg gacgccgaac tgggcgccca cctgccggta gtatttgtct
      241 agatacgcgc gcaggaccgg tggcttgtcg gcgtcaggga tctcgcggcc ggcgattcgc
      301 tcgctgcggc cgccgacccg cagggtcccc tcgcccgccg cgcggacgtt gcgcacccac
      361 tgcgtctcgc ctcgcggcgc caccaggtag cgctgcccac ccacctcgat cgggttgacc
      421 ggtacggtct gaagcttgcc actcttgcgg ccgcgcgcct cgagcacctg cgcgcccgct
      481 ggagacattc caaacctcag aaccaccctc aggcatcata gggactcgag cccaatccgt
      541 catccccttt cgcgaactcc cttgcgtgga tgacgaagac gggatggttc ggggcgatgt
      601 cggcaagctg cgcgagggtc gcgtccttcg gcacgccgaa ctgcgagccg acctcccaat
      661 ggtacctgtc caggtacgcc ttcaggatgg gcggcttgtc gacgtcggga atctcccgcc
      721 cgacgatgtg ctcggctcgc ttgccgaccc gcagcacccc ctcacccgcc actcgaaagt
      781 tgcgcaccca ctgtgtctcg ccgcgcggcg ccaccagata gcggtggcct tcgacctcga
      841 tcgggttgac cggaacggta tgggtcttgc cgctcttgcg ccctcgcacc tcgagcacct
      901 gcgcgcccgc cggcgacatg ccgaacctct tgatgaagaa ctgcaccgcc gggttggcga
      961 ccttcgcggt gaaccagttg ggcttctgat agcaggccat gtagccatcc tgttaacggg
     1021 ctctacgacg cgatatcggg taggggtcaa ccccgcagag tgtcgacccg tagggtatct
     1081 gctacatgcg aacctaccag acgtcgcgtg cgaatcccct acagggggtc cacccctacg
     1141 ggttaaccat tgggccaaag ccccaaggcc atcatgcccg cctttctcgg aaacggcaag
     1201 gcgggcgcta tcgtccgaaa gcacctgccc ttcacgactc gatcagaccc gaacgacttg
     1261 gtcctcgagc ttgcgcgacc gcctggtgag cacctccggc ttgttcttgc cgacgaccac
     1321 agtgtcatca tgccggaacc caccgacccc ccaaatgtag atccccggct ccgaggagaa
     1381 gatctcgcgc tccttgaacg gccggtgcag gtagggcatg tcgtccggga actcgtgccc
     1441 ggcgatgccg atgccgtgtc cggtgcggtg gttgatgtgc tccccgaagc cggccttctc
     1501 gatcacctgc tgtgccgcgg catcggcgga cgagaccggg ttgccggcaa ccatctgcgc
     1561 cgcggcggcc tcgttggcct ccgtcgccgc ctcgaacgcg cgccgctggg tgtcattcgg
     1621 tttgccgagg aagagcgtgc gctcactctc gacgaccaac ccgctcagcc ggaagacgat
     1681 aatgttgacc actccgtggc ccttctcgaa ggtctcgccg acgtccgcgc cggtgccgtg
     1741 cggcgccgcc gaggctggcc cgctcaacga catcatgcgc ggttcgaagc ggtccttggg
     1801 gaagcggcgg gcggcctcct cgatggcgag gctgccgatg tgcaggtcga acgccttgac
     1861 gttcagtccc ggcttgacca gctccatgta cttgtcctgt gcccagtcag tcagcttggc
     1921 gcactcgcgc aggatctcca gctcctcggg gtacttcacc tcgcgcaggg agaccaggta
     1981 ctgggagacg tcctcgaaca cgagcttcgg cgcggccgtg cgcagcgccg ccggaccgga
     2041 gccctcgacc ccgacccggc ccttcgtgat accgcgctcc cccagccgat gggccaccag
     2101 gtcgccgaac tgcagcacgg tccacgtgcg gttggtggtg cgcggatgct cggcgtagat
     2161 cgtgtagtcg gtgatgaaga gcgacttgcg ctcctcggca aacttcaggt ggttggtcga
     2221 gagctcgttc atgatcatga acggctcgcc ctcggccggg atcaccgccg cgacgggccg
     2281 ctcccatggc gcgacatcga ggtggaagcc cgagaccatg tagaaattgt ccggcgtggt
     2341 gacgatgagc gccttgagct cgtgcttccc gagccactcg cggatgacct tcgcgcggtc
     2401 cttgaacacc ttctgtggga cgaacggcat ggattcctcc gttactcggt gatacgcggg
     2461 tcgagcgtgt cccgcagcca gtctccggta atgttcaccg ccagcgcgac gagcgcaagc
     2521 gccagcccgg gcagcgtgat cagccaccag gcagagaaga tgtagtcgag gccctcgctc
     2581 accatgctgc cccacgccgg ggtcggcggc tgcacgccga acccgaggaa actgatgctc
     2641 gcctcggcga tgatgaaccg ggctacctgc agcgtaccga tcacgatcag cggtgtgatg
     2701 atgttcggga agacatgccg caggaaaatg cggccactcc ccgccccgtt cagatgcgct
     2761 gcctgaacga actcccgctc gcgcacgacc aacgtctcgc cgcgagcgac acgggcatac
     2821 tcgatccacc cagcaatgat cagtgtgatg atgatgttcc tgagcccgag cccgaggatg
     2881 gcgttgatcg tcagcgccag caggatgaac ggaaacgaga gctgcacgtc caccagccgc
     2941 atcagaatgg tgtcgatcca gccaccgaag tacccagcca gcataccgat cgggacgccc
     3001 acgagcgccg cgcacagcac cgtgacgaag ccgacgaaca gggagatacg cgcacccagc
     3061 agcatcctgt cccagacatc gcggcccagc tggtccgtcc ctgcgatgtg cccatcgctc
     3121 agtggcggca agagcctcgc accgaagtcg gggctgttca catcgtccgg cacgataagg
     3181 ggcgagacca gcgcaaccac ggtcaccaca accaagaaca ggatcgccat catcgcccac
     3241 gggtccctga gcagcgcgcg gacgaaacgg ttctgccggg ttctcgcccc atcgagcgtc
     3301 tgggccgtgg caaccatcac aatcgcacct ggggattggc ccagccgtag gccagatccg
     3361 ccagcaggtt cgcgagcgtg tacatgatcg cgaagagcac caccgccgcc atcaccaccg
     3421 cgaaatcgcg gttcagcagc gcctgcaccg tgacgcggcc catgccgggc caggcgaaga
     3481 cctgctcgac gatcacggcg ccctcgatca ggcggccgat ctcgagaccg gtcatggtga
     3541 tcagcgagat gctggcgttc ttgagcgcat gacgccagag gatgacctga ccgccgagcc
     3601 ccttcgcttt cgcggtgcgg atgtaatcct gcccgagcac gtcgagcatg gtcgaacggg
     3661 tcagacgggc atatcgagcc gtactgagtg ccgccagcgt gatcgccggc aggatgatgt
     3721 gctccaaccc tccccggccg ctggtcggga gccagccgag ccgcaccgag aagaggagga
     3781 tcagcagcaa tcccagccag aatccgggca ccgcctgccc tgccacagcg atgatccgag
     3841 cgagggtgtc ccagcccgat ccgtgccgcg tggcggagac gatgccgagc gggatcccga
     3901 ccacgatcga aatcagcagc gccacgccac ccagctgcaa cgtcgccggg agtcgttcga
     3961 gcacgatctc cgtcgcaggc cgccgctcgc gggtggaatc gccgaagtca ccctgcacgg
     4021 cgtcggtcat gaagcgcacg tactgcaccg cgagcgggtc atcgaatccc atccgctctc
     4081 gaatctgctc cagatcggtg cgcgcggtat ccataggtgc gaagagcagc acggggtcgc
     4141 cggagaggcg gagcatgaag aagaccgccg tcagcgcgag gaacaccacg atggcgccag
     4201 aaacgaggcg cccgaacaca taggccttca tgcggatatc ccggatggcc ggtatcaggg
     4261 tgatcggtcc ggccgcggga gggcaccatc tctcccggac cggaccgacc cgtcacatca
     4321 gccggaaacg ttacctacct gctgtccgcc aacggaggaa tcaagcctcc cggggttccg
     4381 cctgacccat ccacatgaac tcatccgggc ggggctccca ggcgacatcg ttggtcacgc
     4441 cgtagatgtc gtgctgctgc cagaggaaga gccagcccgc atcgtcgtag acgatctgct
     4501 ggacctcgtt ccagagctcc tgtcgacgct cctcatcgac cgtgaccatc gcctcctcga
     4561 tcagcggcgt gacctcatcg ttgctgtagc tgctgtaggt cgagtcgccg atgaagagga
     4621 acaccagtgt gccgtaagca tcgagcgccg gcccccagcc gaggtagaac atgtcgccgg
     4681 tctgccggtt gacgatcttg tcgaggtgcg tgccccactc gttgtactgc acttcgacgt
     4741 tgatgcccgt atcggtcagc gaggccgcga tcgcttgcgc cgcatcggtg tccatggggt
     4801 agcggccctg cggtgcgtcc atggtcacgg tcagctcgcc cggctcgtag cccgcctcgc
     4861 ggatcagctc gcgagcgagc tcgggatcgt atgggtacgg ctctatctcc gcattcacct
     4921 cgggattcag gttcgagagc gcgcccgcca tcctggtggc gtgtccctgg aacacgccgt
     4981 caatgatggc ctgcacatcg acgccgtggt tcactgccct gcgcagacgg acatcggtga
     5041 aaacgctgtc ctcgcggttg ttgaccagcg ccacgtaatt gacgcgggaa gacggaatct
     5101 cctcgacgcg ggcgttctcg ctgtcctcga cgttcggaat cgaatcgacc gggatgtcct
     5161 tgacgatctg gacctcgcca gccagcagcg ccgccaatcg cgagctgaac tccggcatga
     5221 accggaactc gacctcctgg acctgaacct cgttgtgcca atagtccggg ttgcgctcga
     5281 gccgcaggta ctcgccccgc acccactctt ggaaagtgaa cggaccggtg ccgctcgggt
     5341 tctcgatgta ctcctcgatc ccgatctctt cgatgagcgc ggagtcgacc gggtacaact
     5401 ccaccatgcg ctgtgcgatc aacgggaagg ggtccgtggt cttaatccgg aacgtctggt
     5461 tatcgacgat ggtgacctcc tcgaactgct cccagcgctc caggtagtgg ctctcgttcg
     5521 cgggatcctg cgcccactcg atcgcgtact tgaagtcgtt cgccgttaag gggttgccgc
     5581 tgtggaaggt gatgtccggg ctgaccagcg tcagctccca ggtcaggtca tcgatgttgg
     5641 tgagctcgca caaccacggt cccacctcgt agttctgctc ggggtcatag ttcatcgcgc
     5701 gatcgaacat gtgcgccagc tggatgtcgg tggtccagtc gacgatcgag ttcggcatca
     5761 tggtgagcgg gtcggcgccg agcgcgacca ccacctgctc gagcatgtca tcttgcgcag
     5821 ccgccacctg cgcgctgtag gagagcgcca gcggcgtgcc gagcgcggca gcaaatggcg
     5881 tcgctccggc catcctagcg aaatttcggc ggttcagttc catcgatcgc ttcgtcatgt
     5941 ctcgaccctt tccgaatctg ttctgcacat aactaggacg caagcgtgac ccgtcgccac
     6001 cgaagcaggg gaggcgaacg tgagcgtgtt gcccacacag aagggatcat tgccatggtg
     6061 atccgctgtg tttccggccc gacacccgca ttgtacgctc tggcagcgaa gctagcagtc
     6121 ggaacggctg catgtcaagc acgaacgcct cgaatcgagc cactgagctg ggtagtgctg
     6181 accagcatgc cttcttctgg taccgtgcca gcatcatctc aacgcccagc agtaaggaac
     6241 gactacaaat gtcgcatacc gcctcaccct ctgacgagat tcgcaccgtc accgtgatcg
     6301 gcgcagggtt cctcggtcgc cagatcgccg ccctcgcagc agcctccagg cgcgaggtgc
     6361 gcctctacga cgccaacccg tatgccgccc ccgcggccag aagcctgctg cgcgactatc
     6421 tcgcgtcgcc ggtcgccgac ggcctgctgg actgggatct cgacgaggtg ctggcgcgca
     6481 tccatcccgt ggcgtcgctg gaggaggcca tgcgcggcac cgacctgatg atcgaggccg
     6541 tgcgggagga cctcgagacc aagcgggagg tcttcggagc tgcctccgcc gccaacccag
     6601 acgcctacct cgccaccaac agctcgtcgc tgatgtccgc ccagctacgg gacgtggtga
     6661 gccagactga caagctggtc aacctccact tctttggcga gttctggaac cgctcgatgg
     6721 ttgagctgat gggctgcggc gagacttcgg aggagacaat catcacgctc catcgcttcg
     6781 ggcagtcgct cgggctctac tgcgcggtgg tgcggggcca gagcaaggga ttcatcatca
     6841 accgcgtctg gagagccgtg aagcgagagg ccctgcgcgt ggtcgacgag ggccacgccg
     6901 acccggagga tgtcgaccgc ctctgggcac tcttctgggg cacacagcag gggcccttcg
     6961 gcgcgatgga tcgggtcggt ctcgacgtcg tcgcggacat cgaggacagc tatatcgccg
     7021 tcgccaccga cccgaccgac cgtccctcgc ggatgctgca cgagctggtg gaggcgggac
     7081 gcctcgggat caagactggc gagggattct acgagtaccc gaacccggcc taccgcgcgc
     7141 ccggctggcc ccgcacaagc ccatcgggtg atgattcagc tgggagatga gcccgcaaac
     7201 cagaacgatc ttcactgtaa tgccgttagt tagcgtacaa tagatgctat atggcaaaca
     7261 ttcggatagg agcgccatgg cttggcggca gctcgatcgt cctgctgcgg attcgcgtct
     7321 cgaaaggagc acatgatgtc ccgacagttc cgattcgttc cccatcactc gcggcgacga
     7381 ttcctgcaaa ccagcagtgc cctcgggctc tcggtagtga tgacaagccg gagtgatctt
     7441 gccatcgcgc agtcgtcaac gcccgaagcc gagtccgagc agctctggta cgaggacacc
     7501 cagggaccgc ctgagcccgg cggcacggtt cgcttcctgc tctacgaaga cccgaacacg
     7561 ctcaacccgc ttctgggatc gacatcaatc gctgttcagg tcgtgaccgc cgttctggaa
     7621 gggctcgccg agaacgctcc ggacggcacc tttgtcccgc ttctcgccgc cgagcttcca
     7681 accccagaga acggtggtgt gtccgaggac ctgctcaccg tcacctggaa gctgaaggag
     7741 ggggtgacct ggtcggacgg cacaccgttc accagcgacg acgtgctctt tacctgggag
     7801 gcggcacggg atgcgaccaa cggctcagcc cttgcctccg actacgagtt gatctccagt
     7861 gtcgagacgc cagacgagtt gacggtggtg gtcaactact cgcagttcaa cgccgggtac
     7921 gtggaccagt ttccgtggat cctgccgcgt cacgcaaccg gcccagttga agagatgcgc
     7981 gactggacct tcaaccgcgc gcccatcggc actggaccgt tcaagttcga cgagtggtcc
     8041 tcgggtgagt tcgtccagct gtcggccaat gagaactacc gggaggagga caagccctat
     8101 ctcgatggcc tgctcttcct cgtcgtcccg gacgaatccg tgcggacggc aatgatgatt
     8161 gaggagaatg ccgagatcat gctctggtcc ggtcaggagg ccgatgagca gattactgcc
     8221 gcgggtgtcg gggcatcccg caccgcgccg ggtatctggg tggtcgagat gcgtttcaat
     8281 ctctcacagc ccttcgatga tgacccggga cccgagccgc cacatcccat cctcggtgat
     8341 ccgcgcgtcc gcgaggcgat cacgctcggc gtggatcgcg accggatcac cggcgaactc
     8401 gtaacaggaa ccgtcttcga gctcgactcc ccactcgagg tcggctggat ggactgccag
     8461 gtcgatccct tcgtctacga tcccgagcgt gcacgcgagt tgctcgaaga ggtgggctgg
     8521 cgcgacgagg atggcgatgg tatccgcgag gcacatggcg tcgagacggt cgaggacggc
     8581 accaggcttt ccctgatgat gaacggatac accggcttcg acaccctcga tctcatcgag
     8641 ctggccgtgc aggaggacct cgccaacatc ggcatggaga tcaacatcga gaaccaggag
     8701 ttcgccgtca tcttcggcac ctgggaggat ggatcgcccc ggatgctggg cgactacgac
     8761 atcctcattt acgatgccgg tctcttcgcg gagccaggcg cggatatcgc caatcgctac
     8821 catccggacc gggtgccgag cgctgacaac ccgggcggcg aaaacatcta ccgctgggtt
     8881 cgtgaggacg tcggcgagtg gatcgaggca gccaactcat cgcccgatct cgaggtgcgg
     8941 cgcgagaact tctgcaacgt cgccgaggcg cttcgggagg acatcgtgac cttcccgatc
     9001 ctccagtttt cggagggcag cgcgtattcg aacaggctgc acggcttcac ggtcagcact
     9061 tgggagtggg caacctggga cgccgagaac tggtggctcg agcagtagcc gcgaaacggc
     9121 caccaactcg atcgcagacg ggtgatcccg cctcatcgac gggatcactc gacccctcgc
     9181 tcggcgggag acgatatcgg tgggtcccta catcatcagg cgcgtcctct gggccattcc
     9241 cctgctcctc ttcgtgagcg tcatcgtgta cgggctgctg gagctctcgc ccggcggccc
     9301 gacatccatg tacctgcgcc ggggcacggg catgagcgcc gaggacctgg cacgaatgga
     9361 ggaatcgctc gggctcaacg acccctttta cgagaagtac ggccgctggc tgtggcgggt
     9421 tctgcagggc gatttcggca tggcaaccac ctcgtcccgc ccggtcagtg acgagatcct
     9481 cgagcgactg cccaacacgc tctacctgat gacgatcgcc tgggcggtca gcctgctgat
     9541 agccatcccg atcggcatcg tctccgccgt ccggcagtac tccaagttcg atcactttgt
     9601 gacggcaatg accttcatcg gccagagcat tccgtcgttt ttcctcgggc tgatcctgct
     9661 gctgatcttc tacatgcggc tcgacaaccc gttcagcggc agaccgctcc tgccatcggg
     9721 tggtgtcgaa acgctcggtg ccgacctctc cggctgggac atgatctggg accgcatcaa
     9781 gcatctgatt ctgccggtcg tgatgctctc cgcgacgtgg gtcgcctggt actcccgctt
     9841 tctccgcgcc tcgatgctcg agaccatcca cctcgattac atccgcaccg cccgcgccaa
     9901 gggcgtcgcc gagcgcgcgg tgatcctgcg gcacgcgttc cgcaacgccg cgctgcccct
     9961 cgtcaccctg atggcgctcg acctccccct gctcttcgcc ggcgccctct acgcggaggt
    10021 gatcttctcc tggccaggca tgggccggct gttctaccaa gccgccgagc gccgggacta
    10081 cggactgctg atggctatcg tcatgatcac gtcactactc atcatcgtcg gcaacctgct
    10141 cgccgacgtt gcctaccgct tcctcgatcc gcggatcaag ctccggtagc gcgaggagat
    10201 tctcatggcc ggctcacccg aggcatcggc ttccacgacc acccaaaaca ggcctctcca
    10261 gtcggaggac acgatggcgc ggacggagtc gcgctcgatg ctccggatct acgtccgtcg
    10321 atttctggcc catcggctcg ccgccgccgg cctggtcttc ctcgtgatcg tcaccctctc
    10381 ggccgtgctg gccccggcca tcacgcagga tcccaacgct ctctccgcca gcgatcgcaa
    10441 ggctgcgccg tcggcggacc attggtttgg aacggacgag gcaggacgcg acatcctcgc
    10501 gcggacgatc cacggaggtc gtatctccct cgccgttggg atcacagcca ctgccctcgc
    10561 gctcttcatc ggaacgctga tcggcgctgt ggcggggtac ttcggcgggg ccgtcgacgc
    10621 agcgctcatg cgcctcaccg acgccttcct ctgtttcccg cagctgttcg tgctcatcgt
    10681 cttcggcacg ctcatgcgca cgacggaact gcgtgtcctt cagggcaaca tgacgcctat
    10741 agccatcatc atcggcctgc tctcgtggat gggactggcg cgcatcgttc gtgcgacctt
    10801 cctctccctg cgcgagcagg actacgtcga ggccgcccga tcggtcgggg ccggcaacgc
    10861 ccgcatcatg atccgtcacg tgctaccggg agcagtcggt ccgattgtcg tccaggcgac
    10921 cctgctggtg gcggtgacga tcatcaccga gtccggactc agctacctcg ggttcggcgt
    10981 ccaaccgccg acatcgagct ggggcaacct gctggagggc gccaagccat tcctgcagac
    11041 cctgccggtc tatgcgattg cacccggcat gatgatcttc ctgaccgtga tgagcatcaa
    11101 cttcgtcggg gacgggctgc gcgacgcgct cgacccgcac agtcagcatt gaggcacagg
    11161 tatcatcgaa cagtgcctca cagacatatc atgtcgaccg cagcacaccg agcgcgagac
    11221 gtaaggagac acactgttca tgaaggacta ccggcgctac agcttctggc tcgagacctg
    11281 cggcgacgac ctcacccctc ggccacccct cgatagctcg atcgacgtcg acatcgccat
    11341 cctcggcggc ggcttcaccg gcctctggac ggcctactat ctcctctcga aggagccgtc
    11401 tctcaatgtc gcgatcgttg aagcggagat cgccgggttc ggtgcctcag ggcgcaacgg
    11461 cggctggtgc ttcgccgggt tccccgtctc gccggtaact ctgctgcaga gccacggcta
    11521 cgacgccgcg cgcctcgtct cgctcgagat gtacaaggcg gtcgacgagg ttggtcgtgt
    11581 gacgcgggag gaaggcattg acgcccacta cgccaagggc ggcgaactgg agatcgctcg
    11641 cgcggcgtac gacctgccga agctccagaa gatgtacgac gagtttcgcg cgatcggcct
    11701 cgaggatcac taccggttgc tggacgcgga ggaaaccacc gaacgcatca acgtcgccag
    11761 ggccgccggc agcttccaca accgtgaggg tgcggtcatc cagccggccc gactcgcgcg
    11821 tgggctcgcc cgggcggtcg agcgacgcgg cggaacgatc tacgagcaga cccgcgtgat
    11881 cgatttcacc ggtgcgccca acccctcgct tgtcaccgcg tgggggactg tccgtgcaaa
    11941 gacaattgtc cttgccgggg aggcctatct ctcggcgctg ccaggcatgg accgttcgat
    12001 catcccgatg acctcacaca tcgtggtcac cgagccactc aacgacgaac tctgggaaca
    12061 gattcgctgg tgggatcgtg acacgctggg cgggttcggt gtcaatggtg ggtacatcca
    12121 gcacacggcc gacggccgca ttgctttcgg tccctaccgg ggcaactatc cattcaactc
    12181 acgcatttcc gatgacctcg accgcgacga gaaaatcttc gcccacgccc gaaagtcgac
    12241 gctcgactgg ttcccgatgc tccaaggggt gaagttcacg cacgcctggg gcggcgtgtt
    12301 cggcattcct cgcgaccgca tgcccaccat gaactacgac ccccgcaccg gtatcgccgc
    12361 tgcctatgga tacaccggtg acggggtcgc gacggcgaat ctgtcgggcc gggtgcttac
    12421 cgatctcatc acagagaccg actcgccgct gcaaatgctg ccgatgacac agggtagcca
    12481 acgacgctgg gagccagagc ctctgcgctg gatgggcgtg acattcgttc gcaaggatcg
    12541 tcagcgagtg ctgaagaaaa tcgagcacaa aggcaagcac ccggagaagc cgtcgctctc
    12601 ccagcgcttg tatagctact agccgactcc ctaccattcg aggagaactg ctagcctcag
    12661 agcgccctgg cgatgtccct gacggcgcgc tccagcgtct cccggacgcc aacgccacgc
    12721 acggcgaccg cctcgaccac cggccactct cctgcgccaa tctgcgaggc gatctcgatg
    12781 gtgctgcggc ctgccgcctg gtccatcttg ttgatctgaa agacaaccgg tatctcggtc
    12841 agcgtcctgc cctgccgctc gaggtggtct gcgagctcct ggaggctctc ctgattggcg
    12901 cgaaggcggt ccggactggc atcagcaatg aaggccacgc cgtcgacacc agtaagtatc
    12961 gcgcgccggg tccgggcgta ggattcctgc ccaggtacag agtagatctg gaagcgaagc
    13021 gaccagcccc ccacacgcac cggctcgacc ggcaggaagt cgaagaagag cgtgcgctcc
    13081 tgatcggtgt cgatcgagcg catcggcccg cgcaggtcgc cgggcattcg ctggtagatg
    13141 ttgacgaggt tggtcgtctt gccgctcagg ccgggcccgt agtagacgat ccgcacatcg
    13201 atctcgcggt cggcggtgcg aatcgtcgtc acagatcgtc tcctcgcggc ggacatgcgt
    13261 cgaggggcag gtgtccctca ctgaaacgcc gcagggggcg aatccgaagg gcctcggccc
    13321 gcacaccgtc atccccgcca gtgtgaccgg cggcgccgaa ctcccgcggg cggacacccg
    13381 ggcggagtcc gcttctgcgg ttcaccgcgt gcactgcgat gtctagcacg agagcccctg
    13441 cacggccgac tcgtcgctgg tgacacgcga gatgatcatc ccgatcggtc gcccctccgg
    13501 cagccgcgcg agcatctcgt cgacgtggtc gggggagacg accagcacga acccgactcc
    13561 catgttgaac acgcggtagc gctcggcgac cgagatttga ccacggttca cgagaaagtc
    13621 aagaatctcc ggcgtctccc atacagtcgg gtcaagccga gcgatgcagt cctctgggag
    13681 ggtgcgcggc aggttgtcca gcagcccgcc accagtgacg tgcgccatgc cgcgcacgag
    13741 tccatcctcc agcaacggcg tcacctcgcg cacgtaactg cggtgcggcg ccagcaacgc
    13801 ctcgccgagc gtctcgtcct tggacgcgcc tggcaactgc ttcgcgagca cctcgcggtc
    13861 gtgctccgga tcgccgttca tgccgatgat ccggcgcgca aggctgtagc cgttggtgtg
    13921 gagccccgtg ctgggcaacc cgatcacgag atcgtccggc tcgatggccg agccatcgac
    13981 gatcgcatgc tcctgcaccg ccccgacggt gaagcctgcc agatcgtact cgccatcggc
    14041 gtacatgccc ggcatctcgg ccgtctcgcc gccgagcagg gcgatgccgt tgacggcgca
    14101 ggcgtcagcg atgccgccga caaccccggt gacgacctcg ggcgcgaggc ggccggtcgc
    14161 gaagtagtcg aggaagaaga gcggcctcgc accgagcgcg aggatgtcgt tggcgcagtg
    14221 gttgacgatg tcctcgccca tgcgggcgtg ggcgtcgcct ccgagcgcga acgccaggcg
    14281 gagcttggtg ccgacgccat cggcgctggc gacgaggatc tgtcccggtg cgccgggcaa
    14341 ccggtaggtg ccgccgaagt gtccgatcgg gccggcggtg tcgccgagcg tctgcgcggc
    14401 acgggccttc acctcttcga ttgccgacat ctttgcatcg atgtcgacac cggcatcgcg
    14461 gtaggtgacc ccggtttcag taccgatgtc aggtccagtg cagggcgtgt cgttcactgc
    14521 cacctctcgt cgatgggatt gccggccagc cctcggacac ggggaccggc cggctgcgat
    14581 cgttacgacg cgacgcgatc gcggcgcggt tcctcatcct tgccctcggc tcccggctcg
    14641 gtgtcggggc cggcttcggc gatgtcgtcc ggcacgagcc acggctcgaa gcggccgagc
    14701 tcgcgggcgg gcagctgccc ggtcatggtg gtgccctgct cgtcgtaggc gtgatcctca
    14761 acgcggccga gcttgtggaa gagatcgaca agctccgtac ggtcgaacgg aatcgtcccg
    14821 ctcaccgcca cgaaaccggc ctcctcctcg agcgccgcct cgacggcctc gaagagcctg
    14881 tccaacccga gcccggtccg cgcagagatg ccgacggcgt cgtccggcac gccgaggctg
    14941 ctcgccacct gcggcagccg ctcgcgctca tccggggaga gccggtcagt cttgttgagt
    15001 gcgaagacga ccggccggtc gtcgacggcg aggtcctcca gcgtccggtt gacggtctcg
    15061 acctgctcgg cggcgctcgg gtgagtgcca tcaacgacgt ggacgagcac cgccgcctcg
    15121 ctgatctcct cgagcgtggc gcggaacgcg gcgatcagct gcgtgggtag gttgttgatg
    15181 aagcccaccg tgtcggtgag cagtacctcg cgtccgctcg gcagctggat gcgccgggtc
    15241 gttgggtcga gggtggcgaa gagcttgtcc tcggccagca cctctgcccc ggtgatcgtg
    15301 ttgagcagcg tgctctttcc ggcattggtg taacccacga gcgagaccac cgagatgggc
    15361 gtctgccggc gccgcttgcg gtagcgctcg cggtgctgat gcacctgcgc gagctggtcc
    15421 ttgatgaacg agatgcgctt ctgtgcctcg cgccggtcgg cctccagctg cgtctcaccg
    15481 ggaccgcgca gaccgacacc accgacaccc tgccgggaga ggtgggtcca catgcgggtc
    15541 aggcgtggca accggtactc gagctgcgcc agctcgacct gcaggcgccc ctcgtgggtc
    15601 tgcgcgcggc gcgcgaagac gtcgaggatc agcgccgtcc ggtcgatgat cttgacctcc
    15661 agcgcgcgct cgaggttgcg ctgctgggcg ggcgagagct cgccatcgac catcaccagc
    15721 gtgtagtcga gcgactcccg caggtccctg agctcctcga ccttgccctt gccgacatag
    15781 gccgccggat gcggcgcctt tagcttctga gagacggaac cagcgacctc gacgtcgacc
    15841 gtctcggcga gccgggcgag ctcctcgagg gaatcctgcg cggcccaggc ctgtccctgg
    15901 gtttcgaccg cgaccagcag cgcgcgctcg gtgtcgccga tctgcagggc ggtatcagca
    15961 tcaaatgggg tgactatggc agggtccttt caggcggttt cacggtcatc cgtgcgaaag
    16021 tggctgcaca gcgggccccg tgcagccggg tcaggccgcc aagccgaccc acaggcgcct
    16081 gtcaatcatc acgccagtcc tccagctcag agcagccgcc aaccggtcgc cagtttcctg
    16141 gccggccggc gccccgtcga tctcgatccc cgcgcggagc tgctcgccca tcggcgcgac
    16201 ctcggcccac acgtcgcgtg gggcccgggt ccctacgacc aacgatgtcg atccctcgac
    16261 caccgtgtag cagtcgatgc gaatcacgta ctcctcgccg gcgcgctcct cgtccagcgg
    16321 ctcgaccgtg tagaggatcc aggcatgacc gttttccagc ccgccgggct cctcaccctc
    16381 gacgtcgctg cccacgtcga tgcgcgcgtg ctcctcgtcc tcgcgcagca gctcgagctc
    16441 atcgagcacg cacgcttccg gatcgccccg gtggtccacg atcgtctcga acgtcgcatc
    16501 gatccggtcg gcacgcaggt acaggtaatc atacccacct tcggcaaatg tatgctgcgc
    16561 atcccacaca gtggggttgt agatcactga ccacggcgcc accgggctct cgtacacgcc
    16621 cgggacatca ccgattgccg ccggtgtggc attgcgcccg acctgcagga ggtcggccag
    16681 cggcacgggg ccgtaggtgt cctccgcaag acaggcccac tgcagcacgg cccatgcctc
    16741 gacggcgtcc cggtctacca atgtcgggcc cgttgatcga atagtcatct gcgccgccag
    16801 gtcgcccctc gcaagggtaa cccggtaccc ggaccagcgc ccgccgacgt tggactcgcc
    16861 ggaaatggcc gtcaccacca acctcggttc aacagattcg atgctcacct cgctctcgcc
    16921 ggctgcgaag atgtccagcg gatcgcggat gatgtcgctc tcggcgaacc cggccgcacc
    16981 gctctccgtc tcgaaccgcg cgaccaccac tccgatcgtc acccagctct ggcttgccac
    17041 ttcggccgtc tgccgggcga tcgcgatgtc cgtcgcgccc tccggcagcg acggggctgc
    17101 cagctccccg gaggccagga ccacctcttc gtcgagcatt cgataccagg tatggacgag
    17161 gggtgattcg agcgacaccg cgaccggcac cattcggtcg tcgtcgacca gcatcatctg
    17221 ccggctcagt cccggccctc cgaagcgcag caccctctcg atccggtcga gggtgtcggc
    17281 caccacctcg gcgtgctcga cgggatccgg aggcacctgc cggtaggcca tctccacctc
    17341 gaccacgaac cgaccgatcc gcaggaggat ccgccgggtg tcgtcctgcg aggacactac
    17401 tcgccaagtg accgcttcgc cgagggctgg gccacgctct tcggcgcctt cgggcggcgc
    17461 gccgatcagg agtgtctcca gcacggtcgc ggcgtcgctg ttctccagcg catggatggt
    17521 agtggtcact gcaacgatgg gatcactggc gcggtcggag agaagtacaa agctggcggt
    17581 gtacccctgc cgccatcgcg cgccggtgat gacggtgtcg acgacacctg catcgatggc
    17641 ataacgctgc tcaatgaggt agcgggcatc gtcgcgagtg aggtaggcac cgcgggcgaa
    17701 ctggtagcga tcgtcgggaa gctggtcggg cgtcagcggc atcgtcgcga ggtcaaccgg
    17761 cggcgcaacg tcgccatcct gagccgccgc cggagtcact gcgataccgg ggaagagcag
    17821 caggatgatc agcaaggcgc gccacatgtc gattctctcc ctctagccac ctagagtagg
    17881 ctcgacatgg agtcgacccg taaaggttat ctccatgatc gcccgagcta cggagcggcc
    17941 tcgacggtgg atgtcgatcg gctcgcccac cagcgcgtcc ccaccatcag ccccgtccct
    18001 gcgaagatca gcgccaggct gagccagtac gccgccggcc acgggccgat atagaggctg
    18061 tcggtgcgga tccgctcgat gaacagccgg acgacaccat agatgatcag atagatgccg
    18121 gtgacgtccc ctgcccgaag cgaggcccgc ttcggcacct gcagcacgag ccacgacagg
    18181 aagatcgcat tcaacagatt gaagatcgac tcgtagagga aggtcggatg aaaggtttcg
    18241 aactcggcat aggcgcgggg ccggttctcc ggggcgatct ccaccgccca tggcacgtcg
    18301 gtcggcgtgc cgaaggcctc ctggttggcc cagttgcccc accggccgat cgcctgcgcg
    18361 agcgcgagac tcggagcgat gatgtcagtc catgtcagga acggctggtt gcgccggcgg
    18421 gtataccacc atacggcgag gacaccggcg atgatgccac cgtggatggt gagcccgccg
    18481 agccggacat tgatcgcctc aacgggatgg tcgagatagt agtcccaacg gaggatgatg
    18541 tagtagagcc gagcgccgac cagggcgctg atcaccacca ccggggcgag gtcgaaggcg
    18601 aactcgccgt cgagcccgcg ccgctcggcc agccaggagc cgagcccgat cgccgcaaag
    18661 atgcccgtca ggatgaagag cgcgtaccag cggatatcga gcgcgccgag ggagaaggcg
    18721 gttgggtcat cgagtacagg catgcggatc ctcagtggaa cgtgtgggcc atcgagcggg
    18781 gttggttcaa tccgtatgga ccaacctcgt gtcgaccctc ggcagcttcg ctgccgagcg
    18841 cttcgcgcgg ggattcttcg ctacgctcag aatgacggct ggtgtccgtt gaatggccaa
    18901 cacaaggtcg gcgctacggg tcgatgatcc cacagaaccg gagatcactc gcggtcgcgc
    18961 aggaagacct cgaccgcccg tgccgtcggc aggcctggcc aggcgccagg cttcgtcgtc
    19021 gccagcgcgc cggccgccat cgcgaagcga acatcctcat cggtcaccgg catccagctg
    19081 ctggccagca ggcgggacgt gatcgccgcc gtgaaggcat ccccggcacc ggtcggctcg
    19141 acgacatcga ccgaaaacac tggtatcgat cgtgccgtct tgcgctcgtt tcggtaccag
    19201 acgccccgcc ccccatcggt cacgatggtc gtccgcgccg agtacgacgc cagccgatcg
    19261 agggcctcct gcggagtttc aataccccac acgccgcgag cgtcatccat gctcaccttg
    19321 atcacccggg cactgtgcag gaactgctcg acggcgaggc gtacctgctc cattgaccgc
    19381 cacagtgtcg ggcggaggtt gaggtcgaag accaccggta ccagattcga gacagcgaga
    19441 tcgagcgcgg cgaccgcccc ccgacgcgat tcggaatcgg acagcaccac ggatcccgcg
    19501 accagcgctg acgcctccca cgggcgcgcc gcctcggcat cattgctcga gagcagccgg
    19561 tcggcgaggc ggagcagttg gaactcacca tcgccacgcg cgttcgtcca cgcgagtgcc
    19621 agcgaggtgg cggcctcgga cgtcatccgc agcgcacggg tatctacccc ctcattctgc
    19681 aggatgtcgc gtaagcgata gccaaacgga tcccgaccga caaccgcgca gagcatcacc
    19741 ggcacgccga gccgggccag cgcgacagcg acgttggccg gcgcaccgcc ctcgcggacc
    19801 gcgaaccggt cggcgtgcag gagatcgagc tggttttcgg cgatgagatc gacgagacat
    19861 tcgccgatgg agacgacggg gcggttggga tccaccatcc cggctagctt gcccacgcct
    19921 ggcgggcggt cgccgagttg tcacgctgga cttcaaggac gtcacggacc gcctccagcc
    19981 ggtgcatcac ccggctgagg tggcggatgc cctcgacctc gatggtcagc agcagcgtca
    20041 ccgtgcggtc gtcgtgcgtc tgcgtcagca cggagaggat gttgacgtgg tcctcggcga
    20101 gcatgatgct catgtccttg aggagcccga cgcgatcgag cgcggaaacc cgcaccgata
    20161 ccgggtaagt cgcctggctc tccttgcccc acgagacagg cacgaggcga tccggatccg
    20221 gcaggttggt gacgttggtg cagtcgcggt ggtggtagat gatgccgcga ccgcgcgaga
    20281 tgtacccgac gatcgggtcg cccgggaccg ggcggcagca gttggcctgt gtcgtcaaca
    20341 ggttgccgac cccgagcact tcgacgcggg ccggggacga gggcgccttg acgtgcgtgg
    20401 tggcggtgag tacgtccttc ggcgtttcat cgagccggga gacgatctgc tgtgcggaaa
    20461 cgccaccata gccgatggcg gcgaggaagt catcaagctt gctgtagcgc gggaaaagct
    20521 ccagtacgtc ctcgagcttc ttctcgacac cgaggcgctt gagctcgcgc tcgaggatct
    20581 gcttgccctg cgcgatgttc tcgtcgcgct gctggcggcg gaaccactgg cgaatcttct
    20641 cgcgggcgga cgccgtcttg acgtactcgt cgccggcctg cagccagtca cgccggggtc
    20701 cgctgacggt cttgctggtc ttgatcttga cgacctggcc gttctgcagc ttgtagttga
    20761 gcgggaccat ctggccgttg acggtggcgc tcaccgcgtg gtggccgacc tgcgtgtgga
    20821 tgcggtaggc gaagtcgacc ggcgtcgcgc cattgggaag ctcgatgatg tcccccgccg
    20881 gggtgaagca gtagaccatg tcctggaaaa cgtcggactt gagcgactcg acgaactcct
    20941 cggcgtcggc gacctcgtcc cgccagtcca tgagctgccg cagccacgcg accttggctt
    21001 ccagccgctg gtcggcctga ccaccctcct tgtagcgcca gtgagcggca acgccgtact
    21061 cggcgagctg atgcatctcc ttggtgcgga tctggatctc gaccggctgc cccttcgggc
    21121 cgatcaccgc cgtgtggagg ctctggtaca tgctctcctt cggcgtggcg atgtagtcgt
    21181 cgatctcgcc ggggaccggg tgccagcggc cgtggacgat gccgagcacg ctatagcagt
    21241 cctgcacagt gtcgacaatg atgcggacgc cgatgacgtc gtagatctcg tcgaacgcgc
    21301 gccgcttgcg gttcatcttc cgatagatgg agtagatgtg cttcttgcga gcactcatct
    21361 cggcacggat gccggcggcg gcgatcaggt cctccagctt gcgaaggacc ggcccgatca
    21421 cttcctcggt gccggcaccg tggcgcttga gctccttggt gatgcgagcg tagtcctcag
    21481 gctgaagata gcgaaaggcc aagtcctcga gctcggactt gacctgccag atccccaatc
    21541 ggttggccag cggcgcgtag atctccatcg tctgctgggc gatacgaatc tgcttctcgc
    21601 ggggcatcgc ctcaagcgta cgcatgttgt ggagacggtc ggcgagcttg atgaggacca
    21661 cgccgatgtc gtcgaccatc gctaggaaca tcttgcgcag gctctccgcc tgctgctcct
    21721 tgacgctgcg ctccgtctcg cccggcgccc acttgaggcg gctcagcttg gtgacgccat
    21781 cgaccaggcg ggagacacgc tcgtcgaagc gctcggcgat ctcctcgctg gtgacgtcgg
    21841 tgtcctcgat cgcgtcgtgg agcaacccgc cgacgaccgt ctcggcgtcg aggtgcatgt
    21901 gacagaggaa gattgccacc tcgagcgggt ggacgaagta gggctcaccg gacttgcggt
    21961 tgaagccgtc gtgcgctctt gcagcaagca tcacgccgcg agcgatcatg gcgacatcgc
    22021 cgatgatggt gttggcgtca acagccccgg cgagccggtt gagcgcgtcc aggacccgcg
    22081 gatcgctctg ctcgagattc cgcaggtcaa gtcccgacac cgccgaatcc ggcggatcaa
    22141 gcctggcgag ttcttccaga agcggacgct gagccacgat cgacatcgag cagtctccag
    22201 gtgcgggagc cggcagaccc tgtgttcatc tctttgcgca cattcgcggt gcgcgggagc
    22261 cggagggggc ctgcggaccg ctctcggcag tgccgccggg agcgtgattg atccagtgta
    22321 tgggagatcg atttggggcg caattgcggg atagttcagt tcgtcacaat gacagcgcga
    22381 tacatccgcc cctgccccaa ctgctcggtc catctggccc ttgtgagttc ccgagcccga
    22441 gtatgcagcc ggtacgcatc gcgccccgcc tgaagacacc gggaatggaa agtgcatcaa
    22501 agtgcatcgt ggtaggatgc cgcgagatca gcccccggcc ctgccttggc aggctcgctc
    22561 aggacgccca tgaccgacca tcaggcactc caggagctcg accgcatgcg gccgcccgac
    22621 gaggcgcgca gggtcatcga ggctcacctc cccaccctcc cgaccgagca cgtccccctg
    22681 accgaggcgc tcggtcgcgt gctcgtcgag gatatcgccg caccgttcga ccacccgccc
    22741 ttcccggcat cgactatgga cggcttcgca gtgctggccg ccgacgcctc gccgtggcgc
    22801 gaggtgatcg gcgagcagcc agccggcacg atgatcgccg aggaggtcca cgacgggcag
    22861 gcggtccgca tcatgaccgg cgcgcccatc cccaccggcg cggacgcagt cgtgcccgtg
    22921 gaggagacct ccccggcgga ggatcatgtc gtcatccacg ccgaggacgt cactcccggc
    22981 accaacatcc gcccggtcgg ctccgacctg aaacgagggg aggtcgtcct gcgcaccggc
    23041 acccggctgc accccgccca catcggcctg atcgccagcg tcggcatcgc gcaggtgccg
    23101 gtccgccgcc gcccgcgggt atcaatcctc gcgacgggcg acgaggtgat ggagccgggc
    23161 agcgagctga agccgggaca gatctacgac gccaaccgct tcagcctctc ggcggcggtg
    23221 ctggacgccg gcggggagat cacctggtcc ggcctagccc cggacaggcg tgaccagctc
    23281 gaggcactgc tgcgcgagcg catggagacg gacgacatca tcctgacctc cggcggcatc
    23341 tcgatgggcg agctggacct gatcaaggcg atcctctttg acgctcccga cgtcacggtc
    23401 cacttccgtc ggctcttcat gaagccgggc aagccgctca acttcgccac gcgcggagac
    23461 gcgctgatct tcggcctgcc aggcaacccg gtctcctcgc tggtgacctt cgagctcttc
    23521 ctgcggccgg cgatggcaat catgagcggt gactccaagc cgaaccgacc ccgcgtgccg
    23581 gtgacgctct ccagccctgc tgctccgtcc gaccgcatcg agtatcagcg cgcgagggtg
    23641 acggtgacgc cggaggggaa gctggttggt gccagcaccg ggccgcagca gtcgtcgaga
    23701 ctggcgtcat tcgtcggcgc gaacgcgttg ttggtcattc cgccgcgcga gacgtcctat
    23761 gagcccggcg agatggtcga agccctgctg ctcgcgcctc ccgacagcga gtcgcgctga
    23821 aggttggcga atccatcgat gacgtcattt cgaccgaagc gcctgagagg cgcggagtgg
    23881 agaaatctct tccgccaggg acatcgcttc ccggttcacg ggagatttct cgactgcagg
    23941 ctcgctgacg ctcgccttcc gctcgaaacg acaggtcagg tcgcgggccg gcacaaggcc
    24001 ctcccctaca cgatcacttg cgtcgaaccc tcttcatagc cctccgagga gccccaaccc
    24061 catgccgaaa tccgccaccc tgtccgcccc cgcctggtcc gagaccgaca tcgccgccac
    24121 gcccgtcaca gccgagccgc gcgacggccg cccgatcacc atcgacgacc tctacgcgtt
    24181 ccggatggtc ggcgacccgc aagtgtctcc cgatcggtcg agcatcctca cgaccctgct
    24241 gaccattgac cacgacaccg acgagtaccg cgcggccacc tggcggatgg atgccgacga
    24301 cggcaacacg cgccagctca cgtccggcga gtggcgggac ggctccccga gatggtcccc
    24361 tgacggccgc tggatcgcct tcagctcaaa gcgcggtaac gacgacgcga agactcagct
    24421 ctgggtaatg ccgaccgacg gtggcgaacc gatccgggtg acctcattcg acaacggcgt
    24481 gctcgagcac gactgggcgc cggacagcca gcgcctcgtc gtcgtcagcc tggtcgagat
    24541 gaagccagag cacgcggaga ccgaagccga cgtccgggtg atcacctccg cacaccaagt
    24601 tcaacgggct tggcttcgtc gaccaccgct acccgcaact cttcgtcgtc gaccgtaccg
    24661 agccggacgc cgaaccgctg cagctgaccg agggctggtt cctgcaccgc tccccggcgt
    24721 ggtctccgga cgggcgagag atcgcgttcg ccaccaaccg caaccccgac tgggacacct
    24781 cccgcatctc ggacatctgg accgtcccgg cgcatggcgg tgaaccgcgg cgcctcactg
    24841 acggcacagg gtcgtacgat tcgccggtct ggtcgccgga cggcacccgc ctccccttca
    24901 ctggtgaggc cgaactcgat catgtctacc gcaacactcg cctctggatg a
//
LOCUS       DASYWL010000024        10970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_113721, whole genome shotgun sequence.
ACCESSION   DASYWL010000024 DASYWL010000000
VERSION     DASYWL010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10970
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113721"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1576)
                     /locus_tag="VGR22_00940"
     CDS             complement(<1..1576)
                     /locus_tag="VGR22_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HEV2127168.1"
                     /translation="MTQTDLVFLPYPRSIDLPQDTLGCAIDLSTRLLIGPDAGADTRH
                     AAGTVRAAVREVIGIDVPVTAQSGAAGERPVRFVLQGRDDALLPAGFSDVSGLGFEAQ
                     YTLVVDAQGVTIVAETEAGLFYGAQTLIQVIQQAGRTLPTLRIADAPVLPNRGLMLDV
                     SRFKVPTPEALERLVRTLAHYKYNQLQLYMEHTFASREHPSIAPDAGGLTPNDLLRLD
                     ELCRQHHIDLVPNWQSLGHQRVMLEHPRYAHLAETEWRWSFASTSDEAFEIVDEIYGD
                     LLPNFSSEYFNIDADEPWDMGLGVSKARTDRIGAGELYIEHIRRLHEMVSKHGRTMMM
                     WADVFWHHPEKVTDLPDDIVLLDWWYEPKERYDTVDTLRGRRFYVCPGTGAWLSLFPR
                     IERAIANVQGFVRDGVEAGAEGMLMTDWGDYGHFTMFENNWYPYLWAAECAWSGAETS
                     VEAYDQAVSRLFFRDPSGNLVAAIRRLGSVIAPMDAERQAIAATGPAFWGDPLDRRVE
                     RVLPREQMEQIRQGAEA"
     gene            complement(1847..2860)
                     /locus_tag="VGR22_00945"
     CDS             complement(1847..2860)
                     /locus_tag="VGR22_00945"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2127169.1"
                     /translation="MTEPTQIGAVHVAILSFAHSHANGYAGVLNALPDAELVLIADDN
                     AERGRDAAERFGAEFVGDYVEALARDDVDAVVICSENARHAELTLAAADAGKHVLCEK
                     PLATTMEDAQAMIDACARAGVKLQTAFPVRFNAAAVAFRDAIRNGQAGTPLAVIARNP
                     GTCPMGWFVDPDLAGGGAVMDHTVHVIDVLRHIFDAEVTEVYAEADTRIYDIPVDDTG
                     LVMFRMSNGLRGSLDTSWSRPENWPIWGGVEIEVIGEKGALRLDAFNDNLEIAEIDGP
                     SYTWQAVEHSGDPEMVAAFIHAVQHDEPVQVTGEDGMRAMQVALAAYESVRTHEPVEV
                     VTG"
     gene            complement(2892..3950)
                     /locus_tag="VGR22_00950"
     CDS             complement(2892..3950)
                     /locus_tag="VGR22_00950"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2127170.1"
                     /translation="MNRTSGQHASAAVRFAVIGAGGMGRTHVANIAASPDAGVRWVVD
                     LDEALCTAAAKLCGARASTEMDEALGDPEVDAVLIALPTSAHRMATERAAEAGKHVFC
                     EKPIARHVEDAVAMTEACERADVIFQVGHVVRFFPEYARIKQVLDAGTLGQIAMVRTQ
                     RRSAPVMERSPWFADLEKNGGIIIDLMIHDLDTLRWYFGDVERVFAHGLSYTEWQQSR
                     DVAMASLRFRNGVIAHLDASWAHGGFYTAIEVAGEKGLLSHNSRKNATLTFESSEGTA
                     DKGNIAPQLTFTRPSTFNPYSREVAHFVEAIRTGSPVMTHGAEATRTLALAQSVLDSM
                     RTGRPVVIESNAVATTTA"
     gene            complement(4091..4957)
                     /locus_tag="VGR22_00955"
     CDS             complement(4091..4957)
                     /locus_tag="VGR22_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127171.1"
                     /translation="MPPRPPVPTIADLRNAEPNERRAIAQALVERSHDLETVRAALAV
                     LEAEQDPALRPVLHRKYVWCESSPSRRDSSSFIRAGIVRALQPIIHHDDEPILLRALT
                     TYQMQGLYELCAELRAVALRAMTDLDPGTAALFAARFLSDPLTSFSGEPAITAIHLLA
                     AQQRLEPVFAVASWEKGGGQIVGEALRNLVDLRPELVDLLIERHRESEDAQVVVGLFD
                     LLLHHRERARWHELIIQHVATSQDLGVYGLVVTSIVASRDEVLIGALRALAVDERDPQ
                     RRSLLDHALELA"
     gene            5112..5816
                     /locus_tag="VGR22_00960"
     CDS             5112..5816
                     /locus_tag="VGR22_00960"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV2127172.1"
                     /translation="MPRSHAEPTEHVRELYDGLAPTWNAREARGERLLMGDRFRQELG
                     AMLRGDVLELGSGTGMIFRFVDWDRITSFTATDLSPGMLAEARQRPEVEGRPVTFEQV
                     EATDLPYPDASFDTVTTSLTLCTVPDPERTLREMSRVCRPDGRIVLLEHVDPPNPLLS
                     GVLHLLSPLQERRMGCHLDRPTDRLVERMGFPIERHEGTFLSIFRLLVLCPLPQHRAS
                     GCHRDRLGPSRWHDGC"
     gene            5870..6307
                     /locus_tag="VGR22_00965"
     CDS             5870..6307
                     /locus_tag="VGR22_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127173.1"
                     /translation="MGFVLPKRASINDPVVRGRQPVPGAPGATASPQIPGKVPDRSTI
                     PGEGELPVEVPATPPRRPLTEYGSAQRIMHVFAPEDGNEDDPARFEQAPISYEEMRKA
                     YPTLRSPLRPKPGYALADGIRASSQKAEELAAADTEAEIEPGT"
     gene            6600..7676
                     /locus_tag="VGR22_00970"
     CDS             6600..7676
                     /locus_tag="VGR22_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEV2127174.1"
                     /translation="MASTKRTTKRGRSNDAANSAPEKEKHVELDTEKLLSLYRKMVEI
                     RLFEDAAQRGFRQGKIGGYLHLYVGQEAVATGMLESHQDGDRVITAYRDHAHALLLGA
                     TPREVMAELYGKGTGLVKGKGGSMHLFDVKNDLYGGYGIVGGHIPLGVGMAYAAAYQK
                     EGSIVQLYLGDGAINNGAFHEAANLSGLWGKEGLNPCLFVLENNQYGMGTSVERATAN
                     TDLGSKFDAYGIEHEKVDGMDLVAVIECTRKATQRVRETNTPYAIEALTYRTVPHGAA
                     DFLEKYRTKEEVKKWRERDPIGLLEKRLTDAGVEDSTIEQIHEEKKEVVADAVKFAEE
                     SPEPDLSELTTDVYASEMKVADAG"
     gene            7732..8718
                     /locus_tag="VGR22_00975"
     CDS             7732..8718
                     /locus_tag="VGR22_00975"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEV2127175.1"
                     /translation="MPVKTYRDALRAAMADEMERDESVVLLGEDIGVYGGTHLVTNGL
                     IDEFGPRRVIDTPISELGFTGAAIGMAMAGMKPVVEMMTWNFSFQAADQILQNAAKIR
                     YFSGGQVHIPLVIRGPNGGGVQLSAQHTHSLEGFYGHFPGLKVVSPVTPEDVKGMMLA
                     AIRDPDPVIFLEAGALYGTKGEVPDGDYTVEFGKARVAREGTDVTLIAYGRQVNMLLR
                     VADELKKEGVQAEVIDLRSIRPFDTETIIESVKKTNRAVVVQEQWRWFSVGSEVAALV
                     QEHAFDYLDAPVERVSGAEVPAPYARNLEVAAFPSEAKIQEAVQRVLYQESV"
     gene            8718..10085
                     /locus_tag="VGR22_00980"
     CDS             8718..10085
                     /locus_tag="VGR22_00980"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEV2127176.1"
                     /translation="MAEVIMPKMGDAMEEGTLLKWLKNPGDEVAEGDPIAEIETDKVT
                     LEIEANEGGFLTNRLVEEGDTVPIGTAVATIGSQDEVGKTPDGAGVPAQEAPKAETEG
                     QEEGAGEAAHAAGTDDDAVVPGATNGTAPAAAATPPATEAQEAPAPPERADGSRLRAS
                     PLVKRLAGEHNIDLSQVAGSGPGGRIIKADIAPYVSGEKQAPQAGAPAAQPQAPAQQP
                     AAAPLEAAPGTAVEMSRMKQRTGERMAQSKREVPHFYVTTSVDMSASMEFRKQINASL
                     EEGGGKVSVNDLVIKAAALALAEHPDVNRSYVNGELVQHETIDVNMAVAVEGGLIAPF
                     IPQADQKSLGAISRIAKDIGRRAREGGLKPEEYQGGTFTISNLGMFDVDEFIAVINPP
                     QAAILAVASVRETPVVRDGEIAIGHKMSITLAADHRALDGAEVAVFLQALKRYLENPM
                     LLALS"
     gene            10185..10418
                     /locus_tag="VGR22_00985"
     CDS             10185..10418
                     /locus_tag="VGR22_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127177.1"
                     /translation="MSGFEWFLVIVVVILIPLVVAVAVTLWTLEMARRRNPRNRPGGK
                     VSGTKRRGTRPNAPSDATDAPAASEQEARAGDR"
     gene            10500..10955
                     /gene="ndk"
                     /locus_tag="VGR22_00990"
     CDS             10500..10955
                     /gene="ndk"
                     /locus_tag="VGR22_00990"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEV2127178.1"
                     /translation="MAIERTLVIIKPDAVQRGLIGEIIARFERRGLKIAAMRFETVSR
                     ETAEEHYSEHKGKPFYEGLVEYITSSPSVLMVLEGPDAIAITRTTMGATKPAEASPGT
                     IRADFGLMIGRNLVHGSDSAVSAERETGIFFGGGGIVEYDRAIDVWTRE"
BASE COUNT         1949 a         3618 c         3636 g         1767 t
ORIGIN      
        1 gtgcctcggc accctggcga atctgctcca tctgctctcg cggcaatacc cgctccaccc
       61 ggcggtccag cgggtcgccc cagaatgccg ggccggtggc ggcgatagcc tgccgctccg
      121 cgtccatcgg cgcgataacc gagccaagcc tgcggatagc agcgacgagg ttcccactcg
      181 ggtcccggaa gaagaggcgg cttactgcct gatcgtaagc ttcgacactg gtctccgcgc
      241 cgctccaggc acactctgcc gcccagagat agggatacca gttgttctcg aacatcgtga
      301 aatgcccgta gtcaccccag tcggtcatca gcataccctc ggcaccggcc tcgaccccgt
      361 ctcgcacgaa gccctggacg ttggcgatgg cccgctcgat gcgggggaag agcgagagcc
      421 acgcgcccgt gccgggacag acgtagaagc gccggccccg cagcgtgtcg accgtgtcgt
      481 agcgctcctt cggctcgtac caccagtcga gcagcacgat gtcgtcgggc aggtcggtga
      541 ccttctccgg atggtgccag aagacatcgg cccacatcat catggtgcgg ccgtgcttgc
      601 tgaccatctc gtgcagccgc cggatgtgct cgatgtagag ctcgccggca ccgatcctgt
      661 cggtgcgcgc cttcgagaca ccgaggccca tgtcccacgg ctcgtcggcg tcgatattga
      721 agtactcgga cgagaagttc ggcagcagat cgccgtagat ctcgtcgacg atctcgaacg
      781 cctcgtcgct ggtcgacgcg aacgaccagc gccactccgt ctcggcgagg tgggcatagc
      841 gcgggtgctc cagcatgacc cgctggtgcc cgagcgactg ccagttgggc acgaggtcga
      901 tgtgatgctg ccggcagagc tcgtcgagcc gcaacaggtc gttcggtgtc aggcccccgg
      961 catccggagc gatggacggg tgctcccggc tggcgaacgt atgctccatg tagagctgta
     1021 gctggttgta cttgtagtgc gcgagcgtgc ggaccagccg ctcgagcgcc tcgggcgtcg
     1081 gcactttgaa tcgggagacg tccaacatga gtccgcgatt gggcagcacc ggcgcgtcgg
     1141 cgatccgcag cgtcggcagt gtgcgacccg cctgctggat cacctggatg agcgtctgcg
     1201 cgccgtagaa gagccctgcc tcggtttcgg cgacgatcgt aacgccctgc gcgtcgacca
     1261 ccagggtgta ctgcgcctcg aagcccagtc cggagacgtc cgagaagccg gcgggcagca
     1321 gcgcatcatc gcgtccctgc aggacaaagc gaaccggtcg ctcgccggct gcgcctgatt
     1381 gcgccgtgac cgggacgtca attccgatca cctcgcgaac ggcagcccgc accgtgccgg
     1441 cggcatgccg ggtgtccgca ccggcatccg ggccgatcag caggcgggtc gagaggtcga
     1501 tcgcgcaccc aagggtgtcc tggggcaaat cgatcgaccg cgggtatggc agaaagacca
     1561 gatcagtctg cgtcatggtg tttcctttca tgtcatacat catgtcatac aggcgccggc
     1621 tccttcgtgt cctgacgggc ggcgcttaga tcgcaaccag gagcgacgtc atttcgagcc
     1681 gcgaggcgcg gcacgcgccg agcgtgtcga gaaatgtctt gcatgccaga cgccgtgatt
     1741 cgaccgtcgg agcgatgtct ccactcgcac gccaaggcgc gttccagtcg aaatgacaat
     1801 cgttgattga acggttcggt cgcacgcctg cctgcaccta tcgctcctac cccgtcacca
     1861 cctcgaccgg ctcgtgcgtg cgcaccgact cgtaggccgc cagcgcgacc tgcatcgcgc
     1921 gcatgccgtc ctcgcccgtc acctgtaccg gctcatcgtg ctgcaccgcg tggatgaacg
     1981 ccgcgaccat ttccgggtca ccactgtgct cgactgcctg ccatgtgtag gacggtccgt
     2041 cgatctcggc gatctcaagg ttgtcgttga aggcgtcgag cctgagcgcg cccttctccc
     2101 cgatcacctc gatctcgacg ccgccccaga tcggccagtt ctccggccgc gaccaactgg
     2161 tgtcgagcga gccgcgcaga ccattgctca tccggaacat caccagccct gtgtcgtcga
     2221 cgggaatgtc gtagatgcgc gtgtccgcct cggcgtagac ctcggtcacc tcggcgtcga
     2281 agatgtgccg cagcacatcg atgacgtgga ccgtgtggtc catcacggcg ccaccaccgg
     2341 cgaggtctgg atcgacaaac cagcccatcg ggcaggtgcc ggggttgcga gctatcacgg
     2401 cgagtggggt gccggcctgg ccgttgcgga tggcgtcgcg gaaggcgacc gcggcggcgt
     2461 tgaagcgcac cgggaaggcc gtctgcagct tgacgccggc cctcgcgcag gcgtcgatca
     2521 tcgcctgggc gtcctccatc gtcgtcgcca gcggcttttc gcagagcacg tgcttgccgg
     2581 cgtcggcggc ggcaagcgtc agctcggcat gacgcgcgtt ctccgagcag atcactacgg
     2641 cgtcgacgtc atcgcgggcg agtgcctcga catagtcacc aacgaattcg gcgccgaagc
     2701 gctcggcggc atcccgccca cgctcggcgt tgtcgtcggc aatcagcacc agctcggcgt
     2761 ccggcagggc attcaggacg ccggcatacc cgttcgcatg gctgtgcgcg aagctgagaa
     2821 tggcgacgtg cacagcgcca atctgggtcg gttcggtcat cgggatagct cctcgggatg
     2881 ctgcatcctg gtcaggcggt ggtggtggcg acggcgttgg attcgatcac gacggggcgg
     2941 cccgtccgca tcgaatcgag caccgattgc gccagcgcca gcgtgcgggt cgcctcggcg
     3001 ccgtgggtca ttaccggtga accggtgcgg atcgcttcga caaaatgcgc gacctcacgg
     3061 ctgtaggggt tgaaggtcga cggtcgcgta aaagtcagct gcggggcgat gttgcccttg
     3121 tcggccgtgc cctcgctgct ctcgaacgtg agcgtggcgt tcttgcggct gttgtgcgaa
     3181 agcaggccct tctcgcccgc gacctcaatg gcggtataga agccaccgtg cgcccagctg
     3241 gcatcgagat gggcgatcac gccgttgcga aagcgcagcg aggccatcgc gacgtcgcgg
     3301 ctctgctgcc actcggtgta gctgagcccg tgcgcgaaga cccgctcgac gtcaccgaag
     3361 taccaacgga gcgtgtcgag gtcatggatc atcaggtcga tgataatccc gccgttcttc
     3421 tcgaggtcgg caaaccaggg actgcgctcc atcaccgggg cgctgcggcg ctgggtccgg
     3481 accatggcga tctgcccgag tgtgccggca tcgagcacct gcttgatgcg cgcgtattcc
     3541 gggaagaagc ggaccacgtg accaacctgg aagatcacat cggcccgctc gcaggcctcg
     3601 gtcatcgcga ccgcatcctc gacatgccgc gcgatcggct tctcgcagaa gacgtgcttg
     3661 cccgcctcgg cggcccgctc ggttgccatg cggtgcgctg aggtcggcag cgcgatcagc
     3721 accgcatcga cctccgggtc acccagcgcc tcatccatct ccgtgctggc gcgggcgcca
     3781 cacaatttgg ccgccgccgt gcaaagcgcc tcgtcgagat cgacgaccca gcggacccca
     3841 gcgtcgggcg acgcggcgat gttggcgaca tgggtgcgcc ccatgcctcc cgcgccgatg
     3901 accgcaaacc gcaccgccgc gctcgcatgc tgacctgacg ttcgattcac gtgaactggt
     3961 ccctttccgg catgatggat ggcaagctgt ctcatccgtg ccgagtggga acggatgccg
     4021 catgcaccct acacggttgg catactgcgt acaaggtcga gtccagatgt cgctttcggt
     4081 ccgccaactg tcaagcgagc tccagcgcgt ggtcaagcag gctccgtcgc tgcgggtcgc
     4141 gctcgtcgac ggcgagggcg cgcagcgcgc cgatcagcac ctcgtcacgg cttgcaacga
     4201 tcgatgttac gaccaagccg tagacaccga gatcctgcga tgtggccacg tgctggatga
     4261 tgagctcatg ccagcgagcc cgctcgcgat ggtgcagcag caggtcgaac aggccgacca
     4321 cgacctgcgc gtcctcgctc tcgcggtggc gctcgatcag caggtcgacg agctccgggc
     4381 gaagatccac gaggttgcgc aacgcctccc cgacgatctg cccgcccccc ttctcccatg
     4441 atgccaccgc gaagaccggt tccagccgct gctgcgctgc cagcaagtgg atggcggtaa
     4501 tcgccggctc gccggagaac gacgttagcg ggtcggacag gaaccgcgcg gcaaacagcg
     4561 cagcggtccc ggggtcaagg tcggtcatcg cccggagcgc aacggcacgc agttcggcgc
     4621 agagctcgta gagcccctgc atttgatagg ttgtcagcgc ccgcaacagg atcggctcgt
     4681 catcatgatg gatgatcggc tggagggcac gcacgatccc cgcacggatg aagctgctgg
     4741 aatcccggcg gctgggcgac gactcgcacc atacatactt gcgatgcaac accggccgga
     4801 gtgccgggtc ctgctcggct tcgagtacgg caagtgccgc ccggacggtc tcaaggtcgt
     4861 gcgaccgctc gacgagggct tgcgcgatcg cccggcgctc attcggctcg gcattgcgga
     4921 gatcggcgat ggtcgggacc ggcggacgag gaggcacggg caccctccac agttacggct
     4981 gctttgtagt cactcacggg agtggggcgt ctcatgtccg ccctgcgccc cctttggggg
     5041 atcgccgcat accctccacg gtacactgcc gccattggca accgatgtcg agccccgccg
     5101 ggaggtatcc gatgccccga tcccacgccg aacccaccga gcatgtccga gagctctatg
     5161 acgggctcgc accaacctgg aatgcccgag aggcacgcgg cgagcgcctg ttgatgggtg
     5221 accgctttcg tcaggagctg ggtgcgatgc tccgtggcga cgtgctcgag ctcggcagcg
     5281 gcacgggcat gatcttccgc tttgtcgact gggaccgaat caccagcttc accgccacgg
     5341 acctctcgcc cggcatgctg gccgaggcac gccagcgccc cgaggtggag gggcgcccgg
     5401 tgacattcga gcaggtcgag gccaccgacc tcccctaccc tgacgccagc ttcgacaccg
     5461 tgaccacgag cctgacactc tgcaccgtcc ccgatccgga gcgcaccctc agggagatgt
     5521 cgcgggtctg ccgcccggac ggacgtatcg tgctgctgga gcacgtcgac ccgccgaacc
     5581 cgctcctctc gggggtgctg cacctgctct ccccgctgca ggagcgccgg atggggtgtc
     5641 acctcgaccg tccgaccgac cggctggtgg agaggatggg cttcccgatc gagcgccacg
     5701 aggggacatt cctctccatc ttccgcctgc tcgtcctgtg cccgctgccg cagcacagag
     5761 cgtccggttg ccaccgcgac aggctgggcc cgtcgcggtg gcatgacggc tgctagactg
     5821 ctcgcagcga cagatgcacg cgtgtttgcc accaggaggc ctattcgtca tgggattcgt
     5881 gttaccgaag agagccagca tcaacgatcc ggtcgtgcga ggccgccagc cggtgccagg
     5941 ggctccgggg gccaccgcct cgccgcagat tcctggcaag gtgccagacc gctcgaccat
     6001 tccaggcgag ggcgagttgc cggtcgaggt gccggcgacc ccgccgcgcc gaccgctgac
     6061 cgagtacggc tccgcgcagc gcatcatgca cgtctttgcg cccgaggacg gcaacgagga
     6121 cgacccggct cgctttgagc aggcgccgat cagctacgaa gagatgcgca aggcgtatcc
     6181 caccctgcgc agtcccctgc gtcccaagcc gggatacgca ttggctgatg gaatccgcgc
     6241 gtccagccag aaggcggagg agttggccgc agctgacacc gaagccgaga tcgaacccgg
     6301 cacgtgaaac cacaattcga ggcgatgtct ggccgtcacg ccctccggcg tggcggctgt
     6361 tttgtctacc gagctccgcg gctctcagcg gcagacatgg tgagccaccc cgccggtgct
     6421 gcggtacaat tgcgcccaat tgtggtgaac aaggtcgggg cccggtgtgc ccggctgctg
     6481 tcgagtaaca ctgatgcgcg tgccgcatcg gcaaaaccgc accattgata ccccgtaccg
     6541 ttccgcgagc ggctccgctc ggccgagccg gtgccgcgat agttctcaag aggccggcta
     6601 tggcatccac caaacgtacg acgaagcgag gccggagcaa cgacgcggcc aactccgctc
     6661 cggagaagga gaagcacgtg gagctcgaca ccgagaagtt gctgtcgctg tacaggaaga
     6721 tggtggagat ccgcctcttc gaggatgccg cccagcgcgg cttccggcag ggcaagatcg
     6781 gcggttacct ccacctctac gtgggccagg aggccgtggc caccggcatg ctggagagcc
     6841 atcaggacgg tgaccgcgtc atcaccgcct atcgcgacca cgcccatgct ctcctgctgg
     6901 gcgccacccc gcgcgaggtg atggccgagc tctatggcaa gggcacgggc ctcgtcaagg
     6961 gcaagggcgg atcgatgcac ctgttcgatg tgaagaatga tctctatggc gggtacggca
     7021 tcgtcggtgg gcacatcccg ctcggtgtcg gcatggccta cgcggccgcc taccagaagg
     7081 aaggcagcat cgtccagctc tatctgggtg atggcgcgat caacaacggc gccttccacg
     7141 aggccgccaa cctctccggt ctgtggggca aggaggggct caacccctgc ctcttcgtac
     7201 tcgagaacaa ccagtacggt atgggcacca gtgtcgagcg cgccacggcc aacaccgacc
     7261 tcggctcaaa gttcgacgcc tacggcatcg agcacgagaa ggtcgacggc atggatctcg
     7321 tcgccgtgat cgagtgcacg cgcaaggcga cccagcgcgt ccgcgagacg aacaccccct
     7381 acgcgatcga ggcgctcacc taccggaccg tgccgcacgg cgccgccgac ttcctcgaga
     7441 agtaccgcac caaggaagag gtcaagaagt ggcgcgagcg cgacccgatc ggcctccttg
     7501 agaagcgcct gaccgatgcc ggtgtcgagg attcgacgat cgagcagatc catgaggaga
     7561 agaaggaggt cgtcgcggac gcggtgaagt tcgccgagga gagtccggag ccggatctct
     7621 cggagctcac caccgatgtc tacgcgtcgg agatgaaggt cgcggacgca gggtagccgc
     7681 acgggcagtt cctgggtttt ggaacagctt gctgagaaag ggattggcga gatgccagta
     7741 aaaacctatc gcgatgccct gcgggcagcg atggccgacg agatggagcg ggacgaatcg
     7801 gtcgtcctgc tcggcgagga tatcggtgtc tacggtggca cgcacctcgt caccaacggg
     7861 ctgattgacg agttcggccc ccgccgcgtg atcgacacgc cgatttcgga gctcggcttc
     7921 accggtgccg ctatcggcat ggccatggcg ggcatgaagc cggtggtcga gatgatgacc
     7981 tggaacttct cgttccaggc ggcggaccaa atcctgcaga acgcggccaa gatccgctac
     8041 ttctccggcg ggcaagtcca catcccactg gtgatccgcg gcccgaacgg cggcggcgtc
     8101 caactctccg cccagcacac ccacagcctg gaaggcttct acggccactt ccccggcctc
     8161 aaggtggtct ccccggtcac ccctgaggac gtcaagggca tgatgctcgc cgcgatccgc
     8221 gacccggacc cggtgatctt cctcgaagcc ggtgccctct acggcaccaa gggcgaggtg
     8281 cccgacggcg actacaccgt cgagttcggc aaggcgcgcg tcgcccgtga gggcacggac
     8341 gtaacgctga tcgcctatgg ccggcaggtc aacatgctcc tgcgggtcgc ggatgagctc
     8401 aagaaagagg gcgttcaggc cgaggtgatc gacctgcgct cgatccgccc gttcgacacc
     8461 gagaccatca tcgagtcggt caaaaagacg aaccgggcag tcgtggtgca ggagcagtgg
     8521 cgctggttca gcgtggggag cgaggtcgcc gcgcttgtcc aggagcacgc gtttgactac
     8581 ctcgacgcgc cggtcgagcg ggtcagcggg gccgaggtgc ctgcgcccta cgcgcgcaac
     8641 ctcgaggtgg ccgcattccc gagcgaagcg aagattcagg aagccgtgca gcgcgtgctg
     8701 taccaggaga gcgtgtaatg gccgaagtca tcatgcccaa gatgggcgac gccatggaag
     8761 agggcactct tctcaaatgg ctcaagaacc ccggcgatga ggttgccgag ggtgacccga
     8821 tcgccgagat cgagaccgac aaggtcaccc tcgagatcga ggccaacgag ggcgggttcc
     8881 tgaccaacag gcttgtcgag gaaggcgaca ccgtgccgat cggcaccgcg gtcgccacca
     8941 tcggttccca ggacgaggtc ggcaagacac cggacggcgc gggcgtcccc gcgcaggagg
     9001 cgccgaaggc ggagaccgag ggccaggagg aaggagccgg cgaagcggct catgccgctg
     9061 ggaccgacga tgatgccgtc gtcccgggtg cgaccaacgg caccgcaccg gcagcagccg
     9121 cgaccccgcc ggcgaccgag gcacaggaag ctcccgctcc gccggaacgt gccgacggtt
     9181 cacgcctgcg cgcctccccg ctggtgaagc gcctcgccgg ggagcacaac atcgacctct
     9241 cgcaggtggc cggttccggc ccgggcggtc gcatcatcaa ggcggacatc gctccctacg
     9301 tctctggcga gaagcaggct ccgcaggcag gggcacctgc ggctcagcca caggctccag
     9361 cacagcaacc ggccgccgct ccactggagg ccgcccccgg gacggcagtc gagatgtcgc
     9421 gcatgaagca gcgcaccggc gagcgcatgg cgcagtcgaa gcgcgaggtg ccgcacttct
     9481 acgtcacgac gtcggtggac atgtcggcct ccatggagtt ccgcaagcag atcaacgcgt
     9541 cgcttgagga aggcggcggc aaggtgtcgg tcaacgacct agtgatcaag gcggcggccc
     9601 tggcgctcgc cgagcaccca gacgtcaatc gaagttatgt gaatggcgag ctcgtccagc
     9661 acgaaactat cgacgtcaac atggcggtgg cggttgaggg tggcctgatc gccccgttca
     9721 ttccgcaagc cgatcagaag agcctcggcg cgatctcccg gatcgccaag gacatcggcc
     9781 ggcgcgcgcg cgagggcggt ctcaagccgg aggagtacca gggcggcaca ttcacgatca
     9841 gcaacctcgg catgttcgac gtcgacgagt tcatcgccgt catcaacccg ccccaagcgg
     9901 cgatcctcgc ggtcgcgtcg gtgcgcgaga ccccggtggt gcgcgacggt gagatcgcaa
     9961 tcgggcacaa aatgtcgatc acgctcgcgg ccgaccaccg cgcgctcgac ggcgccgagg
    10021 ttgcggtctt cttgcaggcg ctcaagcgct acctggaaaa cccgatgttg ctcgcgctct
    10081 cgtagacggg caacgacatt acgcgcattg tcgggaacgc cggtggaggc tgatatgctt
    10141 ccaccggcgt tcttcttgta tcggtcgaga acggaaacag ggcaatgagc ggattcgaat
    10201 ggtttctggt gatcgtggtg gtgatcctga tcccgctggt ggtcgcggtc gcagtgaccc
    10261 tctggacact ggagatggcg cggcgacgca acccgcgcaa ccgaccgggc ggcaaggttt
    10321 ccggcaccaa gcggaggggt actaggccga acgcgcccag tgatgcgact gatgcgccgg
    10381 cggcatccga gcaggaggcg cgagccggcg ataggtgaca tcgagctctg cggcccggta
    10441 tgcttgagcg gatatcggag taccggcttg gccgggtagt acctgggaga aagcagaacg
    10501 tggctataga acgcacactc gtgatcatca agccagatgc ggtgcagcgc gggctcatcg
    10561 gggagatcat cgcgcggttc gagcgacggg ggctcaagat cgcggcgatg cggttcgaaa
    10621 ctgtttcacg tgaaacggcc gaggagcact acagcgagca caagggcaag cccttctacg
    10681 aaggtctcgt cgagtacatc acctcgtcac cgtcggtgct gatggtgctg gagggcccgg
    10741 acgcgatcgc gatcacccgg accaccatgg gtgcgaccaa gccggccgag gcgagtccgg
    10801 gcaccattcg agcggacttc ggcctgatga tcggtcgcaa cctcgtgcac ggctctgaca
    10861 gcgccgtgag cgccgagcgc gagactggaa tattcttcgg tggtggcggc atcgtcgagt
    10921 acgaccgcgc gatcgacgtt tggacccgcg agtaaggccc gggccgtgac
//
LOCUS       DASYWL010000025        10314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_226118, whole genome shotgun sequence.
ACCESSION   DASYWL010000025 DASYWL010000000
VERSION     DASYWL010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10314
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226118"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..1434)
                     /locus_tag="VGR22_00995"
     CDS             complement(193..1434)
                     /locus_tag="VGR22_00995"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEV2127179.1"
                     /translation="MRSTLTHLECSNCGESYPADQLMTTCPACGKVLLARYDLDRARQ
                     TMTPEALCSRSWDMWRYTELLPVLDPANRAMLGEGGTPLLEAPHLGKTFGIDRLLVKD
                     EGLNPTGSFKARGLAMAVSRAKELGAEELVIPSAGNAGSAMAAYAARAGLPAHVFMPA
                     DVPEVMKVECHAYGARTYLVDGLINDAGKVSRQAADAFGWFDVSTLKEPYRAEGKKTM
                     GLEIAEQLGWRVPDAIVYPTGGGTGIVGMWKAFDELETIGLIGPARPKMIVVQAEGCA
                     PIVRAFDAGERHAELWIGAETCAAGLRVPVAIGDYLILDAVHQSGGTAITVSETELME
                     GIHVAAEREGIFASPEAGAAFIATRNLRESGFLQQRDEVVIFSTGSGMKHTDMFAPAL
                     PTLDPSADDLLDRIADNARHV"
     gene            1618..2589
                     /locus_tag="VGR22_01000"
     CDS             1618..2589
                     /locus_tag="VGR22_01000"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEV2127180.1"
                     /translation="MTTRGRVEHYQCSVTRPADFDAFWDQILAELDGIPLDPELTPVP
                     LRSTDEVEVFEIHYTSLDDVRIAGWYCRPHERHIAGPYPGLSIVPGYVSEPTLPKSWA
                     KMGYAAVGVAPRGKLRSNSQFNPGYPGLLINRIIDRNTYSYRGFYADAVRAIDFLLSR
                     EEVDSSRIGVHGSSQGGALTIVSAALRPDIVTCGAAGAPYLTGYMDCAELTHSYPYEE
                     MNEYFRLYPERQEQARDTLNYFDCLNLASRVRAPMLLYAGLNDDVCPPETAFALRDAL
                     PGEVTLHTYERCGHDAGNYWEMRNVEAFLAEHLCPATTNEHAQTTVG"
     gene            2600..3607
                     /locus_tag="VGR22_01005"
     CDS             2600..3607
                     /locus_tag="VGR22_01005"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEV2127181.1"
                     /translation="MTEHHERVPQAGTTDAGDTVTTIDAPTGADPKFTSTRPGDLNAW
                     WDGIDEDLAARPANPEYEVAPLQSNEHATVYRPRMTSIDNYVIAAWFSVPHGDGPFPA
                     LFTTPGYASVVTPAHYDLRQRYVTMTVMTRGQRGADKPYAAAFPGHLTNGITDPEEWV
                     FRGVMADILRAWEVLRDHAAVDTRRIGITGTDAGLLLNARRPGAKAVQVTSTFWYRLG
                     ELASETEAYPYEEISDWVRTYPDDRDAVGRSLGYVDPQFQAGKVTAAVHLNRDRESRF
                     LDDAWLAPLVKDIGSELAYYDVTHEGQTDHDANDAWMSDQLGVDPAPRSWQPEEIGPW
                     R"
     gene            3598..4737
                     /locus_tag="VGR22_01010"
     CDS             3598..4737
                     /locus_tag="VGR22_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEV2127182.1"
                     /translation="MAVTDRTTEPRTERDWLAELDIRPVINASATLTALGGSLMPAPV
                     LAAMQAGARHFIDLHELQRKVGARVAELTHNDAAYVSSGAAAGITLSVAACMASADPA
                     LIDSFPGPSGFEKSELVIHRSQRNGYDYAARMTGVTMVEVEGTVEAVEAAITERTAAI
                     LWFAGAHWAEGALPVEQVVAIGRECGVPVIVDAAAQVPTIANLWYFTRDLGADLAIFS
                     GGKGLRGPQSSGLVLGRADLIAAVSINGSPNAAIGRPMKVGKEELLGCLAAVEWSLAQ
                     DEEAVLAGYEATVEMWLDGVRDLPGVIAERGFPSEAGQPHGRAILTIGPDARMSRDAL
                     HEALWERDPRIAVAKIGDDQIALNPQTLEPGEPETILAAIRELLS"
     gene            4917..5369
                     /locus_tag="VGR22_01015"
     CDS             4917..5369
                     /locus_tag="VGR22_01015"
                     /inference="COORDINATES: protein motif:HMM:NF025939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEV2127183.1"
                     /translation="MTPEEKLKDMGIELPEAKPFHPAVELGVVVDNLLFCCGSTPGDK
                     SLKGRVGDVYTTEEGYAAARDCAIQQLAIAKAVLGDLSRIKRVVKLLGMVNAAPGFAE
                     TPQVVHGATEFYYEVLGDKGKHARSAVGMQALPSEVPVEIETIFEIEV"
     regulatory      5506..5614
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            5731..6522
                     /locus_tag="VGR22_01020"
     CDS             5731..6522
                     /locus_tag="VGR22_01020"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2127184.1"
                     /translation="MTQDVALAQVAKAFTRKNQYTDVLDDLSLAVDPGEFVAILGPSG
                     CGKSTVLRLIAGLEQPTAGEVAIGGRPVSVTDDRCAVVFQEPRLLPWRTVAQNVALGA
                     RRRAGNQRERVAALLDEVGLAESAGAWPHQLSGGMAQRVALARGLAAEPDVLLLDEPF
                     AALDALTRLRMQDLLLQVRERHDQTVVLVTHDIDEALYLADRIVILGGTPARVVATHR
                     IPVPRPRDRSHPAIGCMRDSILRRFGVGLVPEREDTVCPGALAAD"
     gene            6623..7654
                     /locus_tag="VGR22_01025"
     CDS             6623..7654
                     /locus_tag="VGR22_01025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042918 - alkanesulfonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV2127185.1"
                     /translation="MTNRLAISSTIDRRRLLAAAGAAAVTGTLWGASRAAAQEEIDAP
                     ETLRMDYAYYNPSSLVLKRFGWIEEALPDTEVTWTLSAGSNKANEFLRSDAIDFASTA
                     GVAAFLARANGTDLHTIYLYSQPEWTALVVTEDSEIETVEDLEGKKIAATKGTDPYFF
                     LLQTLAEHGMSEADVEVVNLQHADGKTALERGDVDAWAGLDPHMAQTELEEGSRLIYR
                     NIDFNTWGALNVREAFLEEYPEVVALVIEQYERARHWILENPDEAAQILSEEAGLSLE
                     VAKKVLVERTNLEIDPVPGETQAEGLEPIIPILEAENQFAPGADPQEALDTLYRTEIT
                     EAVVEGEAP"
     gene            7853..8689
                     /locus_tag="VGR22_01030"
     CDS             7853..8689
                     /locus_tag="VGR22_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2127186.1"
                     /translation="MAIARQTSTIARQPVVPAKPRWRRALGAPRWLLGLIVPVALLGL
                     WQWLVAREVYTRSQLPAPKDVWLGAEQLWTADLLLEHITISIGRVAQGFAWGAGIAVL
                     LGLAVGLSPLIDALLSPTIQAVRAIPSLAWVPLLILWMGIGDQPKITLVAIGVFFPVF
                     TAFVGGIQQIDRKLVEVGHAYGLRGPVLAWQILLPAATPSLLTGLRIGLAQGWLFLVA
                     AELIGASRGLGFLLTDGQNTGRADIIVLSIVLLALLGKGTDVLLGIAQRRALRWSDAW
                     QA"
     gene            8708..9262
                     /locus_tag="VGR22_01035"
     CDS             8708..9262
                     /locus_tag="VGR22_01035"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEV2127187.1"
                     /translation="MVTKNGSTATEAPANRDFKSRQRALKASYAENPELAKQTTVVKS
                     VTPDDAETGKVRIAIDNASAVEVDIGAHCSVGGTEDTGCSGDYFLASLAGCYEVTLRL
                     VASSLKLTINRLDLRVEGDWDARGTLGTDKEAPVGYTEIRVIADVDAEGPEKSLARLG
                     ELTERYCVVSQTLKNPPSVEVTWR"
     gene            complement(9697..>10314)
                     /locus_tag="VGR22_01040"
     CDS             complement(9697..>10314)
                     /locus_tag="VGR22_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEV2127188.1"
                     /translation="VLHTTDRIGEPKAQSARQAIEAINPDVTVIAIEDRLTRENVFDV
                     IEPYDVIVDGSDNFATRYLLNDAAVLRKKPVVHGSIFRFDGQATVLNPSEASPCYRCL
                     FQSPPPPDLAPNCAEAGVLGVLPGMIGMIQATEAIKLVLGIGETLVGRLLMYDARSME
                     FRTLRVKRSPTCSMCGEHGPDSLDGITYDDVSCAIPAAQSASVAE"
BASE COUNT         1749 a         3410 c         3463 g         1692 t
ORIGIN      
        1 ggtggtggtc cgcgtggcag gactgaaaat cagccatgtg cgctacaggg atcctggcat
       61 ggacagcgag acaagcgggg tgttccttcg tttccgaagg acgaagcctt cactaatagc
      121 ggacaggtat gcatgcggac atcgttgtat gcccccgtga ccattacgtg tcctccccgt
      181 cgtgttgggc ggtcagacgt gccgcgcgtt gtcggcgatg cggtcgagca gatcgtcggc
      241 gctcggatcg agcgtgggga gcgccggcgc gaacatgtcg gtgtgcttca tgccgctgcc
      301 ggtcgagaag atgacgacct cgtcgcgctg ctgcaggaac ccgctctcgc gcaggttgcg
      361 ggtggcgatg aatgccgccc ccgcttcggg cgaggcgaag atgccctcgc gctcggcggc
      421 gacgtgtatg ccctccatca gctccgtctc ggacaccgtg atggcggtgc caccgctttg
      481 gtggaccgcg tcgaggatga ggtagtcgcc gatcgcgacc ggcacgcgca accctgcggc
      541 gcacgtctcg gccccgatcc agagttcggc gtgccgctcg ccggcgtcaa aggcgcgcac
      601 gatcggcgca cacccctccg cttgaacgac gatcatcttc ggtcgcgcag gaccgatcag
      661 gccgatcgtc tccagctcgt cgaaggcctt ccacataccg acgataccgg tgccgccgcc
      721 cgtggggtag acgatcgcgt ccggcacgcg ccagcccagc tgctccgcga tctccaggcc
      781 catcgtcttc ttgccctcgg cccggtaggg ctctttgagc gtcgaaacgt cgaaccagcc
      841 gaaggcgtcg gccgcctggc gggaaacctt gccggcgtcg ttgatgaggc catcgaccaa
      901 atacgtgcgt gcgccgtagg cgtggcattc gaccttcatc acctcgggca cgtcggccgg
      961 catgaaaacg tgggccggca gcccggcgcg ggcggcgtag gccgccatcg ccgacccggc
     1021 attcccggcg gaaggaatga ccaactcctc cgcgcccagc tccttcgcgc gggagacggc
     1081 catggccaac cctcgcgcct tgaagctgcc ggtggggttc aggccctcgt ccttgaccag
     1141 cagccggtcg atcccgaacg tcttgccgag gtgcggcgcc tccagcagcg gcgtgccccc
     1201 ctcgccgagc atcgcgcggt tggccggatc gagcaccggc aggagctcgg tgtagcgcca
     1261 catgtcccac gatcggctgc acagcgcttc gggggtcatc gtctgcctgg cccggtcaag
     1321 gtcgtagcgg gcaaggagga ccttgccaca agccgggcag gtggtcatca gctggtcggc
     1381 agggtagctc tcgccgcagt tggagcattc gaggtgggtc agggtgcttc gcatcgggcc
     1441 tccagatggg gtgtccaaac gtcgcccgat cataccccat gtatggctgc atggtagggg
     1501 gtgagacagg ggttcgatcg tgccaggttg tacggaatgc cctcttgcca gacgtggtga
     1561 tccgggcgag agtgccgcca gcgaccatcg ccgcacgcgc tttgaaagga caccagcgtg
     1621 acgacccgtg gacgagtcga gcactatcag tgcagcgtga cccggccggc cgacttcgac
     1681 gcattctggg accagatcct cgccgagctc gacgggatcc cgctcgaccc cgagctgact
     1741 cccgtacccc tgcgctcgac cgacgaggtc gaggtcttcg agatccacta caccagcctc
     1801 gatgatgtcc gcatcgccgg gtggtactgc cgcccgcacg agcgtcacat tgcaggtccg
     1861 tatccggggc tctcgatcgt gcccggctac gtctccgagc cgacgctgcc gaagtcgtgg
     1921 gcgaagatgg gctatgccgc ggttggtgtg gcgccgcgcg gcaagctgcg ctccaacagc
     1981 cagttcaatc ccggctatcc tggcctgctg atcaaccgga tcatcgatcg caacacctac
     2041 agctaccgtg gcttctacgc cgacgccgtc cgcgccattg acttcctgct ctcacgagaa
     2101 gaggtcgatt cgagtcgcat cggcgtgcat ggcagcagcc agggcggcgc gctgacgatc
     2161 gtctctgccg cgcttcggcc tgacatcgtc acgtgtggtg cggccggcgc gccctatctg
     2221 accggctaca tggattgcgc tgaactgacc cattcctacc cctatgagga gatgaacgag
     2281 tacttccggc tctatccaga gaggcaggag caggcgcgcg acacgctcaa ctacttcgac
     2341 tgcctcaacc tcgcgtcccg ggtgagggcg ccgatgctgc tctatgccgg tctcaacgat
     2401 gacgtctgtc cgccggagac cgccttcgcg ttgcgcgacg ccctgccggg cgaggtcacg
     2461 ctgcacacct acgaacgctg cgggcacgac gccggcaact attgggaaat gcgcaacgtc
     2521 gaggccttcc ttgccgagca cctgtgccca gccacaacca atgagcacgc ccagacgacg
     2581 gtgggatagg gggaacgaga tgaccgaaca ccacgagcgc gttccccagg cgggaactac
     2641 cgatgccggc gacaccgtca cgaccatcga cgcaccgacg ggggccgatc ccaagttcac
     2701 cagcacgcgc cccggcgacc tcaatgcctg gtgggacggc atcgacgagg atctggccgc
     2761 gcggccggcg aaccccgagt atgaggtcgc tccattgcag agcaacgagc atgcgacggt
     2821 ctaccggccc cggatgacga gtatcgacaa ctacgtcatt gccgcgtggt tcagtgtgcc
     2881 gcacggcgat ggtccctttc cggcgctctt caccacgccg ggatacgcca gtgtcgtcac
     2941 gcccgcccac tacgacctgc ggcagcgata cgtgacgatg accgtgatga cccgcggcca
     3001 gcgcggtgcg gacaagccgt acgcggccgc cttccccggt cacctgacca acggcatcac
     3061 cgatccagag gagtgggtct ttcggggcgt gatggcggac atactgcgcg cctgggaggt
     3121 gctgcgcgac catgccgccg tcgataccag gcggatcggt atcaccggca ccgatgccgg
     3181 cttgctgctc aacgcccggc gtccgggcgc caaggcagtg caggtgacga gcaccttctg
     3241 gtaccgtctg ggcgagttgg cctcggagac cgaggcgtat ccctatgagg agatcagcga
     3301 ctgggtgcga acgtatccgg atgatcggga cgccgtgggg cgatcgctcg ggtacgtcga
     3361 cccgcagttc caggcgggca aggtgacggc tgcggtccac ctcaaccgag atcgggagag
     3421 ccgcttcctc gacgacgcat ggctggcgcc gctcgtcaaa gacattggtt ccgagctggc
     3481 ctactacgac gtaacccatg aagggcagac cgaccacgac gcgaacgatg cgtggatgag
     3541 cgatcagctc ggcgtggatc ccgcgccgcg gagctggcag ccagaggaga tcgggccatg
     3601 gcggtgaccg accgcacgac agaaccgagg accgagcgag actggctcgc cgagctcgac
     3661 atccggccgg tgatcaatgc ctcggcgacg ctcaccgcgc tcggcggctc gctgatgcca
     3721 gcgccggtgc tggccgccat gcaggccgga gcccggcact tcatcgatct tcacgagttg
     3781 cagcgcaagg tgggagcgcg ggtcgccgag ttgacgcata acgacgcggc ctacgtctcg
     3841 tcgggagcgg cggcggggat caccctttcg gttgcggcgt gcatggccag cgccgacccg
     3901 gcgttgatcg acagcttccc cggcccgtcc gggttcgaga agagcgagtt ggtcatccac
     3961 cgctcccagc gcaacggcta cgactatgcc gcgcgcatga ccggggtgac gatggtcgag
     4021 gtcgagggca ccgtagaagc ggtggaggca gcgatcaccg agcgaaccgc ggccatcctc
     4081 tggttcgctg gcgcccactg ggccgaaggc gccttgccag tcgagcaggt ggtcgcgatc
     4141 gggagggagt gtggtgtccc ggtcatcgtc gatgccgccg cacaggtgcc cacgatcgcc
     4201 aacctctggt acttcacgcg cgatctgggt gctgatctcg ccattttcag tggcggcaag
     4261 gggctgcgcg gaccgcagtc gagcgggctc gtgctgggtc gggccgacct gatcgcggcg
     4321 gtgagcataa acggatcccc gaacgccgcc atcggcaggc cgatgaaggt cggcaaggag
     4381 gagctgctgg gctgcctcgc tgccgtggag tggtcgctcg cgcaggacga ggaggcggtg
     4441 ctggccggct acgaggcgac ggtcgagatg tggctggacg gcgtccgcga tctgcccggc
     4501 gtgatcgccg agcggggttt ccccagcgag gcgggacagc cccatggacg ggcaatcctg
     4561 accatcggtc cggatgcccg catgagccgg gatgcccttc acgaggcgct gtgggagcgt
     4621 gacccgcgca tcgccgtcgc gaagatcggt gacgaccaga tcgcgctcaa cccgcagacg
     4681 ctcgagccgg gcgagccgga gacgatcctc gccgcgatcc gcgagctcct gtcctgaccg
     4741 tcggagccga cccgccggat tgacaggtga acatcgatcc cacaccgtat gggcggcccc
     4801 accgggggtt ggggcgtgcg aagcgcgcga gcgagataca cgcgcctcga gcgctagctg
     4861 gccgatcgac cccgtatacg agatcgaacc tacgtaaatg cgtagaaagg atcgcgatga
     4921 ctccggaaga gaagctgaag gacatgggca tcgagctgcc cgaggcgaag ccgtttcatc
     4981 ctgccgtcga gttgggtgtc gttgtcgaca acctcttgtt ctgctgcggc tccacgccgg
     5041 gcgacaagtc gctcaagggc cgtgtcggtg acgtctacac gacggaggag ggctatgccg
     5101 ctgcccggga ctgcgccatc cagcagcttg cgatcgccaa ggcggtgctc ggagatctct
     5161 cccgcatcaa gcgggtggtg aagctgctcg gcatggtcaa cgccgcaccg ggcttcgcgg
     5221 agacgccaca ggtcgtccac ggcgctaccg agttctacta cgaggtgctc ggcgacaagg
     5281 gcaagcacgc ccgctccgcc gtcggcatgc aggcgctccc gagcgaggtt ccagtcgaga
     5341 tcgagaccat tttcgagatc gaagtgtagc gtcggctcgc atcggcaggg ggcgggccca
     5401 ccaagtgtgg gtccgctcat gccacgctcg tgagcaattt cacgcagggg gttgacggac
     5461 tgcgaccgtt gcccgtagag tgaaaagaac aatcgaatct gaaccgactc atccagagga
     5521 gcagagggaa cggcccgttg aagctccagc aaccccccga cgacgtcgac gggaaggtgc
     5581 taatgccgac agcaatcgtg ctggacgatg aggaggtcaa cggcactccc cgtgagtcct
     5641 ggtgacgtcc cttcgcccac gcgaacgggg cgattttcgt gtctgcggtc cggccagccg
     5701 cggcaccccg gtcgcccggg agtctgaacg atgacccagg acgtcgccct ggcgcaggtc
     5761 gccaaggcgt tcactcgcaa gaaccaatac accgacgtgc tcgacgatct ctcgctcgct
     5821 gtcgaccccg gcgagttcgt cgcgatcctc ggtccgagcg gatgtggcaa gtcgacggtg
     5881 ctccggctca ttgccgggct cgagcagccg accgccggcg aggtcgctat cggcggtcga
     5941 ccggtttcgg tgacggacga ccgctgcgcc gtcgttttcc aggagccgcg cctgctgccg
     6001 tggcggaccg tggcgcagaa cgtcgcgctc ggtgcccgcc ggcgtgccgg gaaccagcgt
     6061 gagcgcgtgg cggcgctgct cgacgaggtc gggctcgccg aatcggccgg tgcctggccg
     6121 catcagctct ccggcggaat ggcccagcgc gtcgcacttg ctcggggact cgctgccgag
     6181 cccgacgtgc tcctgctcga cgaacccttc gccgctctag atgccctcac tcggctgcgt
     6241 atgcaggacc tgctgctgca ggtgcgcgag cggcatgacc aaaccgtcgt gctcgtcacc
     6301 catgacatcg acgaggcgct ctacctcgct gatcgcatcg tgatcctcgg cggcacgccc
     6361 gctcgggtag tggccacgca tcgcatcccc gtgccgcgcc ctcgcgaccg ctcgcacccg
     6421 gcgatcggtt gcatgcggga cagcatcctt cggcggttcg gtgtcgggct cgttcccgaa
     6481 cgggaggaca ccgtgtgtcc tggcgccctc gccgccgact aggcgcgagg ggctgcacaa
     6541 ctccctgatc tctcttgctc acacactgtc gtagcgcagg tcgccgcgat tgtgctccac
     6601 atgcgaaagg atcgatctcg acatgaccaa tcgcctcgcc atttcctcga ccatcgaccg
     6661 ccgccgcctg ctcgccgctg cgggtgctgc cgccgtcacc ggtaccctct ggggcgccag
     6721 ccgcgctgcc gctcaggagg agatcgacgc cccggagacc ctccggatgg actacgccta
     6781 ctacaacccg agcagcctcg tgctcaagcg cttcggctgg atcgaggagg cgctgcccga
     6841 caccgaggtg acttggacgc tgagcgccgg cagcaacaag gccaatgagt tcctgcgctc
     6901 cgatgcgatc gatttcgcct ccaccgccgg cgttgcggct ttcctggccc gtgccaacgg
     6961 caccgacctg catacgatct acctctactc ccagccggag tggacggcgc tggtggtcac
     7021 cgaggactcc gagatcgaga ccgtcgagga tctggagggc aagaagattg ccgccaccaa
     7081 gggcaccgac ccgtacttct tcctgctgca gacgctcgcc gagcatggga tgagcgaggc
     7141 ggacgtcgaa gtggtcaacc tccagcacgc cgacggcaag accgccctgg agcggggcga
     7201 tgtcgacgcc tgggccggac tcgacccgca catggcgcag acggagttgg aggaggggtc
     7261 gcggctgatc taccgcaaca tcgatttcaa cacgtggggt gcgctcaacg tgagggaagc
     7321 gttcctggag gagtacccgg aggtcgtggc cctcgtgatc gagcagtacg agcgcgcccg
     7381 gcactggatt ctcgagaacc cggacgaggc ggcccagatc ctgagcgagg aggcgggcct
     7441 ctcgctcgag gtggcgaaga aggtgctggt ggagcggacg aatctggaga tcgacccggt
     7501 tccgggcgag actcaggcag aggggctgga gcccatcatc ccgattctgg aggcggagaa
     7561 ccagttcgcg cccggtgccg acccacagga ggcactcgat acgctctacc gcacggagat
     7621 caccgaagct gtcgtcgagg gcgaggcacc atagagaggc cgccagaatg ctcaacgtgt
     7681 catttcgacc ggaacgcgcg agcggagtgg agaaatcgct cccatgcgga ggcacaagct
     7741 tcgatcgttg aagcgcattc agacagagat ttctccacgc gggcctgacg gccctcggtc
     7801 gaaatgacac gttgatcagg ggcacaaatc gtttcacgga ggagcaccgg ccatggccat
     7861 cgcccgacaa acgtcgacca tcgcacgtca acctgttgtc ccggccaaac cccgatggcg
     7921 acgcgcactc ggcgcgccgc gctggctgct cggcctgatc gtcccggtcg cgctgctcgg
     7981 cctgtggcag tggctggtcg cgcgggaggt ctacacgcgc agccagttgc ccgccccgaa
     8041 ggacgtctgg ctcggtgccg agcaactctg gaccgccgac ctgctgctgg agcacatcac
     8101 gatcagcatc gggcgggtgg cgcaggggtt tgcgtggggt gcgggcatcg ccgtgttgct
     8161 cgggctggcg gtcggcctct cgccactgat cgacgcgctg ctttcgccga ctatccaggc
     8221 ggttcgtgcg atcccctcgc tggcgtgggt gccgctgctc atcctctgga tgggtatcgg
     8281 cgaccagccg aagatcacgc tggtggcgat cggcgtcttc ttcccagtct tcaccgcgtt
     8341 cgtcggtggc atccagcaga tcgaccgaaa gctggtcgag gtcggccacg cctacgggct
     8401 ccgggggccg gtgcttgcgt ggcagatcct gctgccggcg gcaacaccgt cgctgctgac
     8461 cggcctgcgg atcgggcttg cgcagggctg gctcttcctt gtcgccgccg agctgatcgg
     8521 ggcgtcgcgc gggctcggct tcctcctcac tgacggtcag aacactggcc gtgccgatat
     8581 tattgtcctg agcatcgtgc tgctggcgct gctcggcaag ggaaccgacg tcctgctcgg
     8641 catcgcccag cgccgcgcgc tgcggtggtc ggatgcctgg caggcctgag attgcatgga
     8701 gtagcagatg gtcacgaaga acggatcgac agccaccgag gcgccggcca accgggattt
     8761 caagagccgc caacgggcgc tgaaggcgag ctacgccgag aacccggagc tggcgaaaca
     8821 gacgacggtt gtgaagagcg ttaccccgga cgacgccgag accggcaagg tgcggatcgc
     8881 catcgacaat gcctccgccg tcgaggtcga catcggggcg cactgctcgg tcggcggcac
     8941 cgaggacacc ggatgctccg gggactactt ccttgcgtca ctggccgggt gctacgaggt
     9001 gacactccgg ctcgttgcgt cgtcactgaa gctgaccatc aaccggctcg acctgcgcgt
     9061 cgagggcgac tgggacgcgc gcggcacgct cggcaccgac aaggaggcgc cggtcggcta
     9121 cacggagatt cgagtaattg ccgacgtcga tgccgagggc ccggagaaga gcctcgctcg
     9181 tctcggcgag ctgaccgagc gctactgtgt cgtctcgcag acgttgaaga atccgccctc
     9241 ggtcgaggtg acgtggaggt agcgtccgac cctgtgtcgg gccccgctca cgaatggtga
     9301 cagggtgcgg tcctcacagg caacgccagt tccggtaggc ccgccccgcc gtgggcgggc
     9361 tgcgacatga gcgcctgcgc gcattagccc aagaccacca ccaagggcgg cactccgagc
     9421 gcgtgccaca ggcgcgaagc ctgtcctgag caagggccac aggtccgccg tcgaaggatc
     9481 caagcatccc cgcgcgaagc gctttgcgat agcggtcgtg gcacggtcct ggattcaatg
     9541 tcagagcggc agcaagctgc cgagcgcttc gcgcggggat gcttcgctgc gctcagaatg
     9601 acgatgtcac tggtctcttg gccgccagcg gggcgccacg cgacgtagcc gccccgcagt
     9661 gggcgggcct actgatttct cgatgcgttg cttcgctcat tccgcgacgg acgctgattg
     9721 agccgctgga atcgcacagg agacgtcgtc gtaggtaatc ccgtccagcg aatcggggcc
     9781 gtgctcgccg cacatcgagc aggtcggcga tcgcttcact cgcaacgtgc ggaactccat
     9841 tgagcgcgcg tcgtacatca gcagccgccc gactagcgtc tcgccgatgc cgagcaccag
     9901 cttgatcgcc tcggtcgcct ggatcatccc gatcatcccg ggcagcacac ccagcacacc
     9961 cgcctcggcg cagttcggcg ccagatctgg cggaggcgga gattggaaga ggcagcggta
    10021 gcaggggctg gcctccgaag gattgagcac cgtcgcctgg ccgtcgaatc ggaagatgct
    10081 gccgtgcacc accggcttct tgcgcagcac cgccgcgtca ttgagcaggt agcgcgtggc
    10141 gaagttgtca gacccgtcga cgatcacgtc gtacggctcg atcacgtcga agacgttctc
    10201 tcgagtcagt cgatcctcga tcgcgatcac ggtgacatcc gggttgatcg cctcgatcgc
    10261 ctgtcgcgcc gactgcgcct tcggctcgcc gatgcggtcg gtcgtgtgca gcac
//
LOCUS       DASYWL010000026         9832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_151142, whole genome shotgun sequence.
ACCESSION   DASYWL010000026 DASYWL010000000
VERSION     DASYWL010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9832
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151142"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..236)
                     /locus_tag="VGR22_01045"
     tRNA            complement(162..236)
                     /locus_tag="VGR22_01045"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(202..204),aa:Lys,seq:ttt)
     gene            333..1136
                     /locus_tag="VGR22_01050"
     CDS             333..1136
                     /locus_tag="VGR22_01050"
                     /inference="COORDINATES: protein motif:HMM:NF014049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HEV2127189.1"
                     /translation="MDRLVVSPIEFAIAVAAAAVIAALALRKRLLGPGGAVTAPLLGP
                     GLVAAGDWWLGALLIGFFLTSSLLPSTEQGGPARTAWQVLANGGPALVFGTLAVLTGE
                     PAFLIGAATTIAAATSDTWATELGKAFGGTPLSIRSGQRVPPGTSGAVSGAGTGASVA
                     GAVVIAALAWWLSPLAPEAATLGAAQVAVIAACGVLGSAIDTLLGGTLQARFRCDRCG
                     KTSESPSEHELGHRAQHASGVAWMTNSTINLIAVTSAGSTAVALGMLAL"
     gene            1312..2901
                     /locus_tag="VGR22_01055"
     CDS             1312..2901
                     /locus_tag="VGR22_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEV2127190.1"
                     /translation="MQKALATDIDHTISLRAVPIVLVALIIGILLAFAPAAQAQGSTP
                     AVDATPAASPEVAAVGTNALGNVLMSYEFLEMPQVPMTARLLRITLAPGASVPMHTHP
                     GIEFDYVESGTLTAVPNGTMVISVDGEQSQVTESQTLSEGSGVMYPPETGMSLRNEGD
                     EDVVLLSAVLLSVGNDVESTITYTEGEPTDTDFEGVSFVVLGDGLIQQFPDAPATVAV
                     DRLTVDAGAPVPGFSGVAMLSKESGSLAFEATEGAVQVTRTENPQLQPNAIPGQEFEL
                     NDNDAAFFPAGYETIQRPDSQDSLTLVRLLVLPEGDLAAEPAVVTALPTEVAATDDGE
                     ATNAGDGFGVGAIVALNVDAVNIRAEATTDSEIVDTFPAGTQFEITGNPVEGESYTWY
                     PVQGVGDLSSVEGWLVTDFMDVIDSAPEGESQTDGDGSSGTQDEAEATPEAASALEAA
                     GGEVEIGATVQVTEDTVRVREEPNAGAFIINTYDTGTEMEVLDGPVQADGFSWYQVEV
                     LETGVVGWTAVDFLEVVEPAQ"
     gene            complement(3000..3842)
                     /gene="aroE"
                     /locus_tag="VGR22_01060"
     CDS             complement(3000..3842)
                     /gene="aroE"
                     /locus_tag="VGR22_01060"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEV2127191.1"
                     /translation="MRRCGLIGNPVEHSLSPVMQTAAFRETGIGGSYELWPTTDSEVP
                     ARVAELREPDVLGANVTVPHKQQVMPEIDEISETARRIGAVNTIINRGGHLVGDNTDA
                     YGFARVVASTGFGASPESRALVLGAGGAARAVIVALQQAGFGTVEIANRTPERAATLA
                     RELDREGTVSPVLWGDLETVLSSVDLLVNATSLGWHVGELPLDIARLGLLSERATVVD
                     LTYRDTDLLQVARRRGLMAVDGLGMLIHQGARAFELWTGVEAPVEAMRAAVEIEQRGR
                     AGMA"
     gene            complement(3856..5007)
                     /gene="mltG"
                     /locus_tag="VGR22_01065"
     CDS             complement(3856..5007)
                     /gene="mltG"
                     /locus_tag="VGR22_01065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEV2127192.1"
                     /translation="MVRILTQSLKIITIAVLSVFAVSGAVWFFDYWQDRSTPQDEGYP
                     VLLQVSEEDDSGSVADKLKDTELINFGFYFETRMRFSDVQLQPGVYTLRRGMSVPEII
                     DAITIEAVATGEEVEDSGRDAEVIEVTLIEGQRAEQFAAAIEEAGYPNGREVFMNAVM
                     DPSVRSGWDFLEGVPADASLDGFLFPDTYTFTTDYTGEDMANMLLQNFDAKVTEQTRD
                     AWRDQGLSAYEAVTIASIVEREAAVTEERATIAAVYLNRIAQGMLLNADPTIQYVVGT
                     PEEWWPVLTNADLEVDSPYNTYIYEGLPPTPISNPGLQSLQAVGQPENVDYLYFFALQ
                     DGSGEHVFASTYEEHQQNVCAVDPVACEGASAPTVGGGFAGTRPLTGAA"
     gene            5125..6087
                     /gene="miaA"
                     /locus_tag="VGR22_01070"
     CDS             5125..6087
                     /gene="miaA"
                     /locus_tag="VGR22_01070"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEV2127193.1"
                     /translation="MAGANAIDTSTPNPPLIVLTGPTATGKTAAAIRLAAELPVEIIN
                     GDSRLFYRGMDIGTAKPTREERTVVPHHLIDTLDPTETMTVARFQDEVYALIEEIHDH
                     HRLPVLVGGTQQYINAVIEGWRIPRVPPEPALRARLQQEADEHGRDYLLARLRALDPD
                     AAAPIGSNLRRIIRALEVIEVTGRPVSEQQGKTEVPFAPLLIGLTMPRDLLYERIDRR
                     VEEMLDAGLIDEVRALLHAGVPEDAPALSSIGYRQVLPHLHAEAAVEEVAEHIRNDTH
                     RLVRHQQTWLRKSAGLVEIDVSSEGWFERLRDHIARHIARHRVP"
     gene            6174..6629
                     /locus_tag="VGR22_01075"
     CDS             6174..6629
                     /locus_tag="VGR22_01075"
                     /inference="COORDINATES: protein motif:HMM:NF022466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HEV2127194.1"
                     /translation="MHDYAFRDAQRVSKLALSIAWHAGLTREVVTTAGERLAIIFPGH
                     WTHGHGPDFRNAMLEGDDGRLLIGAAELHHRATDWTRRGHHTDPAYNDVVLHVVTEPD
                     TSETRRLDGALVPMAVLHIPETQLRAVEKRRPEIWDRFGGRRLCRSPRP"
     gene            6592..7557
                     /locus_tag="VGR22_01080"
     CDS             6592..7557
                     /locus_tag="VGR22_01080"
                     /inference="COORDINATES: protein motif:HMM:NF022466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HEV2127195.1"
                     /translation="MGGDVCAARLVREQPDRIRSLLRDLSDTRFGQRVLRFESELSLM
                     PPTAALTSALFEAFGYSRNREQMLRLARAVDWTSFAPRLHRLDRDSRTRLTLAALLGL
                     SGWMPLRPSHASIAHLAPSEVTRLETIWEAEHASWAHAALTGTIWDIARVRPANHPVA
                     HVATLAVLLGGHGTELVPVLLNAIRDKLPLEEILLQIVSGTSAPPLGADRAIAIAASA
                     VLPFAVAYARLYGDDALEDDALRAWGALPAGSPSQSARRARQQVAGDVRITGFRERGA
                     QGLLWLDRHYCGPRRCYECPIARAVVVDELAQPNSSMNTGSSSAC"
     gene            complement(7512..9242)
                     /locus_tag="VGR22_01085"
     CDS             complement(7512..9242)
                     /locus_tag="VGR22_01085"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEV2127196.1"
                     /translation="MEDIRIILDLVMALGVATIAGVAADRVGIPVLLGYVAAGMAIGP
                     HTPGLFANEDRVMQLANLGVAFLMFTIGAELSLRDLLAVRRVALIAATIQMPLAFAFG
                     LLTGRLIGWDAPASVLLGAIFTISSSIVLIKVVLNRGEATSRQARYGIGLMVVQDLSL
                     VPILALLPVLEGETGNIVFEIGRSLALASVALAIVLVLGTRLVPALLYRVARTSSHEL
                     FLLTIVTIALGTALASHYAGLSLALGAFLAGLVVSESEFDAQVMVEIAPLRDLFATLF
                     FVSLGMLVQPAVIADQPLVLLLMAVTLIGGKLLAGTMGFLASRINPFVAVSAGFLSAQ
                     IGEFSFVLAGSGLESGIITSEQYGLVIALALISILIAPGLPSLAVPVASVINRFSFAR
                     DVELETLETENQSVELRRHVVICGYGRVAAELGAALERRGAAYVVIDINAATVRELRR
                     RGIPAIYGDAGRRSVLEHAGVAEARTVAVTVPDLLAASAAIRFARELNPTVDIIARAG
                     VRGEVFTLRADGASEVVQPEFEAGLEFTRYVLRRLGVSARELELVSARRRSRYYQQAE
                     DEPVFIDELG"
     gene            9493..>9832
                     /locus_tag="VGR22_01090"
     CDS             9493..>9832
                     /locus_tag="VGR22_01090"
                     /inference="COORDINATES: protein motif:HMM:NF040248.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2127197.1"
                     /translation="MGRYILGRLLGLAFVLFVVSLIAFLLMHSVPGGPFAPDEKGRLP
                     EATRQAQLEKYGLDQPIYVQYVKYMWNALQLDFGVPFQSPTETVTGLIARAWPVTIKI
                     GVPTIILAYTL"
BASE COUNT         1742 a         3405 c         3101 g         1584 t
ORIGIN      
        1 ctttagcacc ttacccggac tccctcggca gccatccgca tggacgccaa acgattgttt
       61 cagcgacctt ttgtcgtcat ttttgtcgtc agaaggcatg cagcatgatg gccggcacgc
      121 ccttcggcgc ccgtctgccc agtagatact ggattttccg gtggtgaccc ccctgggatt
      181 cgaacccaga accaacggat taaaagtccg ctgctctgcc gttgagctag agggtcccgc
      241 aactcgacag aatcgcgcgg gtcaatccta ccgcgcccca tcccgctgtg ccacactccc
      301 acagaccgtc atcgacgccg cccgggagca tcatggaccg cctcgtcgtc tcgccaatcg
      361 agttcgccat cgcggtggcc gcagcagcgg tcatcgccgc gctcgccctt cgcaagcggc
      421 tcctcggtcc cggtggcgcc gtcaccgcgc ccctcctcgg accgggcctc gtcgccgccg
      481 gcgactggtg gctcggtgcg ctgctcatcg gcttcttcct cacctcctct ctcctgccat
      541 cgaccgagca gggtggaccc gcccggaccg cctggcaagt gctcgccaac ggcggcccgg
      601 ctcttgtctt cggcacacta gccgtgctca ccggcgagcc tgccttcctg atcggcgcgg
      661 cgacgaccat cgccgccgca acctccgaca cctgggcaac cgagctcggc aaggcgttcg
      721 gcggcacacc gctctcgatc cgcagcggcc aacgagtacc accgggcacg tccggagcag
      781 tgagcggcgc gggaaccgga gccagcgtcg ccggcgccgt ggtgatcgcc gcgcttgcat
      841 ggtggctctc gccccttgcc ccagaggcag ccaccctcgg ggccgcacag gtggccgtca
      901 tcgccgcctg cggcgttctc ggcagcgcga tcgatacgct tctgggcggt acgctgcagg
      961 cccgatttcg atgcgatcgg tgcggcaaga cctccgaatc gccgagcgag cacgagctgg
     1021 ggcatcgagc gcagcatgcc agtggcgtcg cgtggatgac gaacagcact atcaatctca
     1081 tcgccgtcac gtccgccggc tccacagcag tcgcgctggg aatgctggca ttgtgaccgc
     1141 gatcgttcac gacgccgctt cgtgccagcc gccctaatgg agtaagattc gaaaaaacgc
     1201 tggcagcgca tcgcgcgacc gtccgagggg cccgtccaga cacctatctc ggctgtctct
     1261 cgcaccgaac cggtcccggt ctcatcggac acgatccttg tgaggagcat catgcagaaa
     1321 gcattggcga ccgacatcga ccacacgatc agcctgcgcg ccgtgccgat cgtgctcgtg
     1381 gccttgatca tcggcatcct gctggcattc gcaccggccg cgcaggcaca gggatccacg
     1441 cccgcggtcg atgccacgcc cgctgcgtct ccggaggtcg ccgcagtcgg gaccaatgcg
     1501 ctcggcaacg tactcatgtc ctatgagttc ttagagatgc cgcaggtccc gatgacggct
     1561 cgcctgctgc gcatcacgct cgctcctggc gccagcgtgc cgatgcacac ccaccccggc
     1621 attgagttcg actacgtcga gtccggcacg ctgaccgcag ttcccaatgg cacgatggtc
     1681 atcagtgtcg acggagagca atcccaggtg accgagtccc aaaccctctc cgagggatcg
     1741 ggggtcatgt acccacctga aaccggcatg agcttgcgga acgagggcga cgaagacgtc
     1801 gtgttgctct ccgcggtgct gctttccgtg ggcaatgacg ttgagtcgac gatcacctac
     1861 accgagggtg agcccaccga cacggacttc gagggagtct ctttcgtggt cctcggcgac
     1921 gggctgatcc agcaattccc ggatgcgcca gccaccgtcg ccgtcgaccg gctcaccgtc
     1981 gacgcgggcg caccggtgcc cgggttcagc ggcgtggcga tgctcagcaa ggaatctggc
     2041 tccttggcgt tcgaggctac cgagggcgcg gtccaggtga cgcgcaccga aaacccgcag
     2101 ctccagccga acgcgattcc cgggcaggag ttcgagctca acgacaatga cgctgccttc
     2161 ttcccggccg gctacgagac catccagcgt cccgacagcc aagattccct cacgctggtg
     2221 cgcctgctgg ttctgccaga gggcgatctg gccgctgaac ccgcggtcgt caccgccctc
     2281 ccgaccgaag tggcggcgac cgacgatggg gaagcgacga acgcgggtga tgggttcggc
     2341 gtcggcgcga ttgtggcgct caacgtcgac gcggttaaca ttcgcgccga ggcgaccacc
     2401 gatagcgaga tcgtcgacac attcccggcg ggcacgcaat tcgagatcac tggcaacccg
     2461 gtcgaaggcg agagctacac gtggtatccg gtccagggtg tcggcgacct ctcctcggtc
     2521 gagggctggc ttgtcaccga cttcatggac gtgatcgatt ccgcgccgga gggcgagtcg
     2581 cagacagacg gcgacggcag ctccggcaca caggacgagg ccgaagcgac gccggaggcg
     2641 gcgagcgcgc ttgaagcggc cggcggcgag gtggagatcg gcgcgaccgt gcaggtgacc
     2701 gaggacacgg ttcgggttcg cgaggagccg aatgccggcg cgttcatcat caacacctac
     2761 gatacgggca ccgagatgga agtgctcgac ggcccggtgc aggcagacgg cttcagctgg
     2821 taccaggtcg aggtgcttga aaccggcgtc gtagggtgga cggccgtcga cttcttggag
     2881 gtcgtggagc cggcgcagta agttaccgtt tcagccgaac atggagtaac gatgcggcgg
     2941 ctcggtcgag ccgccgcatc gtctgtcgtt caacatcggg gcgggtgaat agggatcgct
     3001 catgccatcc cggcacggcc gcgctgctcg atctccacag ccgcacgcat cgcctcaacc
     3061 ggcgcctcga cgccggtcca gagctcgaac gctctcgcgc cctggtgaat cagcatgccc
     3121 aagccgtcga ccgccattag gcctcggcgg cgtgcgactt gcagcaggtc ggtgtcgcga
     3181 taggtgaggt ccacgaccgt cgctcgctcc gagagcagcc cgagtcgcgc gatatcgagc
     3241 ggcagttcac cgacatgcca gccgagggat gtggcgttga ccagcaggtc caccgaggac
     3301 agcaccgtct ccaaatcgcc ccagagcacc ggggacactg tgccctcgcg gtccaactct
     3361 ctcgccagcg tagccgcccg ctccggcgtg cggttggcga tctccaccgt gccgaacccc
     3421 gcttgctgca gcgccacgat caccgcccgt gccgcgcccc cggccccgag cacgagcgca
     3481 cggctctccg gcgaggcacc gaaaccggtc gacgccacga cccgcgcgaa cccgtaggcg
     3541 tcggtgttgt cgccaaccag gtggcctccc cgattgatga tcgtgttgac cgcaccaatg
     3601 cgtcgcgcgg tctcggagat ctcgtcgatc tcgggcatga cttgctgctt atgcggtacc
     3661 gtcacgttcg cgccgagcac gtcgggctca cgcagctcgg ccacgcgagc tggcacttcg
     3721 ctgtccgttg tgggccaaag ctcgtagctg ccgccgatgc cggtctcgcg aaacgccgcc
     3781 gtctgcatca ccggcgagag cgagtgctcc accgggtttc cgatcagtcc gcagcgtcgc
     3841 atcggcgctc ctctctcaag ctgcgccagt caaggggcga gtgccggcga acccaccgcc
     3901 gacagtcgga gcggacgccc cctcacaggc cacgggatcg accgcgcaga cgttctgctg
     3961 gtgctcctca tacgtgctcg cgaagacatg ctcgcccgag ccgtcctgca gggcgaagaa
     4021 gtagaggtaa tcgacgtttt cgggctgccc gaccgcctgc agcgactgaa gacccgggtt
     4081 ggagatgggc gtcggcggca gaccctcata gatgtaggtg ttgtacggcg agtccacctc
     4141 gagatcggcg ttggtcagga ccggccacca ctcctccggc gtgcccacga cgtactgaat
     4201 cgtcgggtcg gcgttgagga gcatgccttg cgcaatgcgg ttgagataga cggctgcaat
     4261 ggttgcgcgc tcctccgtga ccgccgcctc acgctcgacg atcgaggcga tcgtgaccgc
     4321 ctcgtacgcg ctgagcccct gatcacgcca cgcatcgcgg gtctgctcgg tgaccttcgc
     4381 gtcgaagttc tggagcagca tattggccat gtcctcgcca gtgtagtcgg ttgtgaaggt
     4441 gtacgtgtcc gggaagagaa acccgtcgag gctggcgtcg gcgggaacgc cctcgaggaa
     4501 gtcccagccg gagcgcaccg acggatccat gaccgcgttc atgaagacct cgcggccgtt
     4561 cggatagccc gcctcctcga ttgccgcggc gaactgctcg gcccgctgcc cctcgatgag
     4621 ggtgacctcg atcacctcgg cgtcgcggcc ggagtcctca acctcctcgc ctgtagcgac
     4681 cgcctcgatc gtgatggcgt cgatgatttc cggcacactc ataccccggc gcaacgtgta
     4741 gacaccaggc tggagctgga cgtcggagaa gcgcattcgt gtctcgaagt agaagccgaa
     4801 gttgatcaac tcggtgtcct tgagcttgtc cgccaccgag ccgctgtcat cctcctcact
     4861 cacctgaaga aggaccgggt agccctcatc ctgcggcgtt gagcgatcct gccagtagtc
     4921 gaaaaaccag actgcgccgg acacggcgaa gaccgagagc acggcaatgg tgatgatctt
     4981 caggctttgg gtgagaatac gaaccaacgc gagcgtctcc cgtgaggtgt gatgccacca
     5041 tggagcacgg gcatacgatt cggggctccc atggtcaagg cacatgcagc aggcgacagc
     5101 gcgcccgggt tttaaccagt gtaaatggcg ggagcgaacg cgatcgatac atcaacaccg
     5161 aatccgcctc tcatcgtact gacaggtccg acggcgacgg gcaagaccgc cgccgcgatc
     5221 cggctcgccg ccgagttgcc agtcgagata atcaacgggg actcccgcct cttctaccga
     5281 ggcatggata tcggcaccgc caagccgacg cgcgaagagc gaaccgtcgt gccccaccac
     5341 ctgatcgaca ccctcgaccc aaccgagacg atgactgtgg cccgctttca ggacgaggtc
     5401 tacgcgctga tcgaggagat ccacgaccac catcgcctcc ccgtgctcgt ggggggcacc
     5461 caacagtaca tcaacgcggt gatcgagggg tggcgcatcc cccgggttcc gccggagccg
     5521 gcgctgcgcg cccgcctgca acaggaggcc gacgagcacg gacgggatta cctgctcgct
     5581 cgcctgcgag cactcgatcc ggatgcggcg gccccgatcg ggtcgaacct tcgccgcatc
     5641 atccgcgcgc tggaggtgat cgaggtgacc ggccgcccgg tctccgagca acagggcaag
     5701 accgaggtgc cgttcgcgcc gctgctgatc ggcctcacca tgccacgcga tctgctctac
     5761 gagcgcatcg accgccgggt ggaggaaatg ctcgacgccg gactgatcga cgaggtgcga
     5821 gccctgctcc acgccggtgt cccggaggac gctcctgccc tctccagcat cggctaccgg
     5881 caggtgctgc cccacctgca cgccgaggcc gcggtcgagg aggtcgccga gcacatccgc
     5941 aatgacaccc atcggctggt gcgacaccag cagacctggc tgcggaagtc cgccggactc
     6001 gtcgagatcg acgtgtccag cgagggctgg ttcgagcgcc tgcgcgacca tattgcccga
     6061 cacatcgcgc ggcatcgtgt cccctgagta ccgcttgaca cgggttgatc ctcgccctac
     6121 gctgcagaga gttacaccag accaacgtcc gaaagaatcg ccggaggacc gggatgcacg
     6181 actacgcctt ccgtgacgcc caacgggtga gcaaactggc gctcagcatc gcttggcacg
     6241 ctggtctcac gcgcgaggtc gtcaccaccg caggagagcg gcttgcgatt atcttcccgg
     6301 gtcactggac gcacggtcac ggccccgatt ttcggaatgc gatgctcgag ggggacgacg
     6361 ggagactcct cattggtgcc gccgaactgc accatcgcgc gaccgattgg actcgccgcg
     6421 gtcaccacac cgatcccgcc tacaacgacg tggtgctgca tgtcgtcacc gagccggaca
     6481 cgtctgagac ccggcggctc gacggtgcgc tggtgccgat ggcggtcctg cacatccccg
     6541 aaacgcagtt gcgcgccgtc gagaagcgtc ggccggagat ctgggaccgc tttggggggc
     6601 gacgtctgtg ccgctcgcct cgtccgtgag cagcccgacc gaattcgcag cctgctccgt
     6661 gacctcagcg atacgcgctt tggacagcgc gtcctccggt tcgaatcgga actcagcctg
     6721 atgccaccaa ctgcagcgct cacatctgca ctgttcgagg cgttcggcta ctctcggaac
     6781 cgtgagcaga tgctgcgcct cgccagggcg gtcgactgga cgtctttcgc tccaaggctc
     6841 caccgcctgg atcgtgattc gcgcacccgc ctgaccctcg ccgcgctgct cggcctcagc
     6901 ggctggatgc cgctgaggcc ctcacacgcc agcatcgcac acctcgctcc gtcagaggtg
     6961 acccgcttgg agacgatctg ggaggctgag cacgcgtcat gggcccacgc agcgctgacg
     7021 ggcaccatct gggacatcgc ccgggtccga ccggcgaacc acccggtcgc gcacgtggcg
     7081 accctcgcgg tgcttctcgg cggtcacggc accgagctgg tacctgtgct gctcaacgcc
     7141 attcgcgaca agcttccgct cgaagagatc cttctacaaa tcgtgtccgg gacgtccgct
     7201 cccccgcttg gagcagaccg cgccattgcg atcgccgcga gcgccgtgct acccttcgcc
     7261 gttgcctacg cgcgcctata tggagacgat gcactggaag acgatgccct gcgagcgtgg
     7321 ggcgcgctgc cggctggatc tccgtcgcaa tcggctcgac gggcgcgaca gcaggtcgcc
     7381 ggtgacgtgc gcatcaccgg gttccgcgag cgcggcgccc agggcttact gtggctcgac
     7441 cgacactact gcggaccccg gcgctgttac gagtgcccta tcgcccgagc cgtggtcgtc
     7501 gacgagcttg ctcagcccaa ctcatcgatg aacaccggct cgtcttccgc ctgttgataa
     7561 tagcgggacc ggcgccgtgc cgagaccagc tccagctctc gcgccgaaac gcccaggcgc
     7621 cgcaggacgt accgcgtgaa ctccagccca gcctcgaact ccggctgaac cacctccgag
     7681 gcgccatcgg cccgcagcgt gaacacctcg ccgcgcacgc cggcacgggc gatgatatcc
     7741 accgtcgggt tgagctcccg cgcgaaccgg atcgcggccg acgccgcgag caggtcgggc
     7801 actgtcacag ccacggtccg cgcctccgcg acgccggcat gctcgagaac cgaccgtcgc
     7861 ccggcatcgc cgtagatcgc cgggatgccg cgccgccgca gctcgcgaac ggtggccgcg
     7921 ttgatgtcga tgacgacata ggcagccccg cgccgctcca gcgccgcgcc cagctcggcg
     7981 gcgacccggc catacccgca tatcacgaca tgccgccgaa gctcgacgga ctgattctcg
     8041 gtctcgagcg tctccagctc tacatcccga gcaaaggaga atctgttgat cacgctcgcg
     8101 acaggcaccg ccagcgacgg gagccctggc gcgatcagga tcgaaatcaa cgccagtgcg
     8161 atcacgagac cgtattgctc cgacgtgatg atgccgctct ccaggccgga gccggccagc
     8221 acaaagctga actcgccgat ctgtgcggag agaaacccgg cggagacggc gacgaagggg
     8281 ttgatgcgcg acgccaggaa acccatcgtg ccggccagca acttgccgcc gatcagcgtc
     8341 accgccatca gcagcagcac cagcggctgg tcggcgatca cggctggctg caccagcatc
     8401 ccgagcgaga cgaagaagag cgtggcgaag aggtcgcgca gcggtgcaat ctccaccatc
     8461 acctgcgcgt cgaactccga ctccgaaacg acgaggccgg cgaggaacgc tccgagcgcg
     8521 agcgagagac cggcgtagtg gctcgccagt gccgtgccga gcgcgatggt gacgatcgtc
     8581 agcagaaaga gctcgtgcga gctggtgcgc gcgacgcggt agaggagtgc cggcaccagg
     8641 cgcgtgccga ggaccaagac gattgcgagc gccacgctcg ccagggccag cgagcgtcca
     8701 atctcgaaga cgatgttgcc ggtctcgccc tcgagcacgg ggagcagcgc gaggattgga
     8761 acgagcgaga ggtcctgcac gaccatcaac ccgatgccat agcgagcctg ccggctggtt
     8821 gcctcgccgc gattgagcac caccttgatc agcacaatcg agctggagat ggtaaagatc
     8881 gcgcccaaga gaacggacgc cggcgcgtcc cacccgatca gacgaccagt aagcagcccg
     8941 aacgcgaagg cgagcggcat ctgaatggtc gccgcgatca gcgcgacacg ccgcacggcc
     9001 agcagatcgc gcagcgagag ctcggcgccg atcgtgaaca tgagaaaggc aacaccgagg
     9061 ttcgccagct gcatgacgcg atcctcgttg gcgaaaagcc ctggggtgtg cgggccgatc
     9121 gccatcccgg ccgccacata gccgagcagc actgggatgc cgacccggtc ggcggccacc
     9181 cccgcaatcg tcgccacacc cagcgccatc acgagatcca ggatgatgcg gatgtcttcc
     9241 acggggcgct cgactccggg tcgcgaccgc cggcctcttc ccgacgcacg cgcgtactct
     9301 cctcctcagg aagtgtaccc gcacgatgcg tgcgggtagc ctgctccgcc gaccgccccc
     9361 ttgcgacatc gcgctatctc cgaaacaata gtcaccacac aagcaagatc gacgttacgg
     9421 tgcagtgacg gtcagggtcg cgggtggctt cacccgcgtt tccaacgctt tatccgtgga
     9481 ggcacggctt cagtgggtcg ctacattctc ggccggttgc tcggcctggc attcgtcctg
     9541 ttcgtggtct cgctgatcgc gttcctgctc atgcattcgg tcccgggcgg accgtttgca
     9601 cccgatgaga aggggcgcct gccggaagcc acgcgccagg cgcagctgga aaagtatggg
     9661 ctcgaccagc cgatttacgt tcagtatgtc aagtacatgt ggaacgcgtt gcagcttgac
     9721 ttcggcgtgc ccttccagag tccgaccgag acggttaccg ggctcatcgc gcgggcttgg
     9781 ccggtcacaa tcaagatcgg cgtcccgacc atcatcctcg cctacaccct cg
//
LOCUS       DASYWL010000027         2866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_432701, whole genome shotgun sequence.
ACCESSION   DASYWL010000027 DASYWL010000000
VERSION     DASYWL010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432701"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VGR22_01095"
     CDS             complement(<1..326)
                     /locus_tag="VGR22_01095"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HEV2127198.1"
                     /translation="MATNEMLVAWMNDAHALEKGIVETLETQVEAASDHPEVKRGIEE
                     HLEATKTHVRLVEECLETLGEKPSTVKEGMATITSKAQGMMMGGAKDKLVKSALNDYS
                     TEHMEIA"
     gene            541..1854
                     /locus_tag="VGR22_01100"
     CDS             541..1854
                     /locus_tag="VGR22_01100"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HEV2127199.1"
                     /translation="MEHNASGEQQAPPETDNEIREAVRSMLKSNTHEGYSQRFGTSFC
                     YIQPSPGTYPYQYFWDTCLHVFILTALDEHELAMRNMRSLFAMQDEDGYVGHMLYWGR
                     VRPAKWTDIFQSRPTRHIFQQHMSALIQPPLVAQTVQRIHMGTQDDAFLGEMLPKLKR
                     YFDWLANHRDLDDDGLLSIITTFESGMDWKPTFDQVVGFPHGVANRRLFWRVVATDAR
                     NFLNRYNIETLKRKRYFLVKEVAFNTIYAQNLRALAQLCEALDDPDAQRYHSMAERVA
                     ASMIEHMYDEEEAAFYDIDGRDGSKIRVLTPTIFFPLVVRTMPDRITDTLIERHFLQE
                     SEFDTPFPIPSVALDDPSFYPHESEYLWRGPTWVFYNWFVYQCLFYRGYREEANALQT
                     SIKRLIRQSGFREYYNPFSGAGHGAHNFTWSGLVVDMLNLTEETM"
     gene            2055..>2866
                     /locus_tag="VGR22_01105"
     CDS             2055..>2866
                     /locus_tag="VGR22_01105"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2127200.1"
                     /translation="MASLADSLDQPSRIGTGALIRRFAAYYRPHRRLFTIDFGSAVVS
                     GLLELAFPIAVQIFIDDLLPGQDWGLITLAAAGLLLIYLLNTGLMAVVNYWGHMLGIN
                     IETELRRRSFDHLQKLSFRFYDNNRTGHIIGRVTKDLEDIGEVAHHGPEDAFIAVMTF
                     VGAFALMFAINVELALITTLIVPFTAWLTTRYGGRLTRTWRSLFSSVGEFNTRIENNV
                     SGMRVVQAFANEDHERDLFAVDNANYRRRKLRAYKLMATSMSLSYFSMRFTQL"
BASE COUNT          506 a         1013 c          823 g          524 t
ORIGIN      
        1 gcgatctcca tgtgctccgt ggagtagtcg ttgagcgcgc tcttgaccag cttgtccttg
       61 gcgccgccca tcatcatccc ctgcgccttg gacgtgatcg tcgccatgcc ctccttgacg
      121 gtggacggct tctcgcccag cgtctccagg cactcctcga cgagtcgcac gtgggtcttc
      181 gtggcctcga ggtgctcctc gatgccgcgc ttgacctcgg gatggtcgga cgccgcctcg
      241 acctgcgtct ccagggtctc gacgatgccc ttctccaacg cgtgcgcgtc attcatccat
      301 gctaccagca tctcgttagt cgccatgtgg cacctcctgc gagcggggcg ttcggcaccc
      361 cgctgattgg ctcatccgtg caggccgcgt gccacggctt cccggcgacc gtcgaatcag
      421 gacggcccga cacgatcgcg gtcgagcggt gctcggggaa cagttcttcc gatacgggcc
      481 actggcacgg atacggccca cacatacggg catcgcccgg tcggcaagga ggaaccccca
      541 atggaacaca acgcctcggg agaacagcag gcgccaccgg aaacggacaa cgagatccgg
      601 gaagcggtgc ggtcgatgct gaagtccaac acgcatgagg gctattcgca gcgattcgga
      661 acatcgtttt gctacatcca gccctcaccc ggcacctacc cgtaccagta cttctgggat
      721 acctgcctgc acgtcttcat cctgacggct ctggacgagc acgagctggc gatgcggaac
      781 atgcgcagcc tgttcgccat gcaggacgag gacggctacg tcggtcacat gctctactgg
      841 ggccgggtcc ggcctgccaa gtggactgac atcttccagt ctcgcccgac caggcacatc
      901 ttccagcagc acatgagcgc cctgatccaa cccccgctcg tggcccagac ggttcagcgc
      961 atccacatgg gcacgcaaga cgatgcgttt cttggggaga tgctgccgaa gctgaagcgc
     1021 tacttcgatt ggctcgccaa tcaccgcgac ctcgacgacg atggcctgct gtcgatcatc
     1081 accaccttcg agtccggaat ggactggaag cccaccttcg accaggtggt cggcttcccg
     1141 cacggcgtcg ccaatcggcg gctcttctgg cgagtggtcg cgacggacgc ccgcaacttc
     1201 ctcaaccgct acaacatcga gacgctcaag cggaagcgct attttctggt caaggaggtg
     1261 gccttcaaca cgatctacgc gcagaacctg cgggcgctcg cgcagctctg cgaggcgctc
     1321 gatgatcccg atgcgcagcg ctaccattcc atggcggagc gcgtcgccgc gagcatgatc
     1381 gagcacatgt acgacgagga ggaggcggcg ttctacgaca tcgacgggcg tgacgggtcg
     1441 aagatccggg tgctgacgcc caccatcttc ttcccgctgg tcgtccgcac gatgccggac
     1501 cgcatcaccg acaccctgat cgagcggcac ttcctgcagg agagcgagtt cgacacgcca
     1561 ttcccgatcc cctccgtcgc cctcgacgac ccgtccttct acccgcatga gtccgagtac
     1621 ctgtggcgcg ggccgacctg ggtcttctac aactggttcg tctaccagtg cctcttctac
     1681 cgggggtacc gggaggaggc gaacgcgctg cagacgtcca tcaagcgtct catccggcag
     1741 agcgggttcc gcgagtacta caacccgttc agcggtgcgg gccacggggc gcacaacttc
     1801 acctggtccg gactcgtggt cgacatgctg aacctgaccg aggagacgat gtgatacgca
     1861 gcagagtggt gctgcgtgaa gcacctaggc atcgttgcca gcgtctgtcg taggggcgga
     1921 cacgaggtcc gcccagtgcg cgcgaagcgt gcacttgccg cgcgccgccc ggtcggctac
     1981 atatgagtgg cttaccggcg gccagacgtc gtcggcattt tgtcatgccg cgctttcaac
     2041 tccgacagga caccatggcc tcgcttgccg acagtctcga ccaacccagc aggatcggca
     2101 caggggcgct catccgccgc ttcgccgcct actaccggcc gcaccgccgc ctcttcacca
     2161 tcgacttcgg ctcggcggtc gtttccggcc tgctcgagct cgccttcccc atcgccgtcc
     2221 agatcttcat cgacgacctc ctccccgggc aggactgggg cctgatcacc ctcgcagccg
     2281 ccgggctgct gctcatctac ctgctcaaca ccggcctgat ggcggtcgtt aactactggg
     2341 gccacatgct cggcatcaac atcgagaccg agcttcgccg ccgcagcttc gatcacctgc
     2401 agaagctctc cttccgcttc tacgacaaca accgtaccgg tcacatcatc ggtcgcgtca
     2461 ccaaggatct cgaggacatc ggcgaggtcg cccaccacgg ccccgaggac gccttcatcg
     2521 ccgtgatgac cttcgtcggc gccttcgcgc tgatgttcgc catcaacgtc gagctcgccc
     2581 tcatcaccac cctgatcgtc ccgttcaccg cctggctcac cacccgctac ggtggccgcc
     2641 tcacccgcac ctggcgcagc cttttcagca gtgtcggcga gttcaacacg cggatcgaga
     2701 acaacgtcag cggcatgcgc gtcgtgcagg cgttcgccaa cgaggatcac gagcgcgacc
     2761 tcttcgccgt cgacaacgcc aactaccgca gaaggaagct ccgcgcctac aagctgatgg
     2821 cgaccagcat gtcgctcagc tacttcagca tgcgcttcac ccagct
//
LOCUS       DASYWL010000028         3042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_451597, whole genome shotgun sequence.
ACCESSION   DASYWL010000028 DASYWL010000000
VERSION     DASYWL010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451597"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="VGR22_01110"
     CDS             <1..798
                     /locus_tag="VGR22_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2127201.1"
                     /translation="ALSGDWEQALEVNDAFLQRFPNDPEALNRKGHALLELGRLQEAW
                     DAYAEALAADPANMIARRNLQRLEMLVNTDVSQRAGAERIASPRAHVFIEEVGKTWVD
                     ELTHAAENAVLATVSPGKQLAFEVQNGRVMVSSRTGQVLGELEQRIAHRLADLIDAGN
                     QYEIYALGMSGHSLRIIIRETHLDPSMEGQQGLPRQSRQTMDLMRERELLSQREEGDF
                     SFGDEDEEELGEDEAEVDEEDEEVEVDEEAAQYVDSSLADDSEEDAM"
     gene            complement(962..1258)
                     /locus_tag="VGR22_01115"
     CDS             complement(962..1258)
                     /locus_tag="VGR22_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127202.1"
                     /translation="MPPAHRNTSRAAERRRQRRQRRSGQEGSQRSSQVPKMAPLQLDP
                     RRAGASSSAPVAVPTAKLEPDMEFAIVRRDLWRLTLYSIICFALMIAVLYVLNA"
     gene            complement(1277..1639)
                     /locus_tag="VGR22_01120"
     CDS             complement(1277..1639)
                     /locus_tag="VGR22_01120"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HEV2127203.1"
                     /translation="MAADVESLNLLQRAQLVWSLMRDDRVSGWVKKAAPAAIIAYVIS
                     PIDVIPDFLLGAGQVDDVGVVAVGLVLLARLLVRFAPRDVVEQHVSRITGTWDGQRSR
                     TGRPDGPTIDTRGRVRQQ"
     gene            2058..2345
                     /locus_tag="VGR22_01125"
     CDS             2058..2345
                     /locus_tag="VGR22_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127204.1"
                     /translation="MNSSMIGKIEKAHRYAREPERITFETFTASFRGSHDDYTVSLTA
                     NGWECTCHTFETHMLGTCSHIMAIQHILGGMLPDRARYDSAEAETLEAVAG"
     gene            complement(2342..2608)
                     /locus_tag="VGR22_01130"
     CDS             complement(2342..2608)
                     /locus_tag="VGR22_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127205.1"
                     /translation="MTLGRVSWRTVAATWGGAVMLVTRQFGVPASGSAIGETRIVRIA
                     EAGELRAAAERTADGRILTLPIEPGSDHQRQDDRRLSDCRFAVN"
     gene            complement(2605..2964)
                     /locus_tag="VGR22_01135"
     CDS             complement(2605..2964)
                     /locus_tag="VGR22_01135"
                     /inference="COORDINATES: protein motif:HMM:NF017920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEV2127206.1"
                     /translation="MKLIIAIIQAYDSDRLLRAVTSAGLGATKIVSTGGFLRMANATI
                     LMGVDDERVQQCLEIIREHCEPRVESAIDPGEQEFIEWFPIGVHDVTVGGAVVFILPV
                     RDYRRIFPAGHREEGQP"
BASE COUNT          550 a         1020 c          996 g          476 t
ORIGIN      
        1 gccctgtccg gcgactggga gcaggcgctg gaggtcaacg acgccttcct ccagcgcttc
       61 ccgaacgacc cggaagcact caaccgcaag ggccacgcgc tgctggagct cggccgcctg
      121 caggaggcat gggacgccta cgccgaggcg ctcgccgctg acccggcgaa catgatcgct
      181 cgccgcaatc tgcaacggct cgagatgctg gtaaacaccg acgtctccca gcgggcaggc
      241 gccgagcgga tcgcctcccc gcgcgcgcat gtcttcatcg aggaggtcgg caagacctgg
      301 gtcgacgagc tgacccacgc cgccgagaac gccgtgctgg cgactgtctc ccctgggaag
      361 cagctggcgt tcgaggtgca gaacggcagg gtgatggtgt cctcgcgaac cgggcaggtg
      421 ctcggtgagc tcgagcagcg catcgcccac cggctcgctg acctgatcga cgccggcaac
      481 cagtacgaga tctacgccct cgggatgtcc ggccacagcc tgcggatcat tatccgagag
      541 acccatctgg atccttcgat ggagggacag caaggcctgc cgcgccagag ccgacagacg
      601 atggacctca tgcgcgagcg cgagctgctc tcgcagcgcg aggaaggcga cttctcgttc
      661 ggtgacgagg acgaggaaga gctcggcgaa gacgaggcgg aggtcgacga ggaggacgag
      721 gaagtcgagg tcgatgagga agcggcccag tatgtcgata gctcgctggc cgatgactcc
      781 gaagaagacg cgatgtagcg cacctcttct cgcagcgata accgataatc cccgacccga
      841 aactcggcgg ggatcgacat tggtatgact gttgacggcg ataccgtgcg gttgtgggga
      901 gagcggcggg tcggattctt ccctggagac ccggtcgacg ctctccaggc cgtttgaagg
      961 ttcaggcgtt gaggacgtag agcaccgcga tcatcagcgc gaagcagatg atcgagtaga
     1021 gagttagccg ccacaggtcg cgccggacga tcgcaaactc catgtcgggc tcgagcttcg
     1081 cggtcggcac cgcgaccggc gccgacgatg acgcgcccgc acgccgggga tcgagctgca
     1141 gcggcgccat cttcggcacc tggctgcttc gttgcgagcc ctcctgcccg cttcgtcgct
     1201 gccggcgctg gcgacggcgc tcagcggccc tcgaggtgtt gcggtgtgct ggtggcatgg
     1261 tctgtcctgt tccgggctac tgctggcgga cgcgcccccg cgtgtcgatc gtcgggccgt
     1321 ccggtctgcc ggtccgcgaa cgctgtccat cccatgtgcc ggtgatgcgg gacacgtgct
     1381 gctccaccac gtcccgcggg gcgaaccgca ccagcagccg tgccagcaac accaggccga
     1441 cggcgaccac accgacgtcg tcgacctggc cggcgccgag caggaagtcc ggaatcacgt
     1501 cgatcggcga gatgacgtag gcgatgatcg cggcaggggc agctttcttg acccaccccg
     1561 acacgcggtc gtcgcgcatc aggctccaca ccaactgggc gcgctgcagg agattgagcg
     1621 attcaacatc ggccgccatc tggtcacctc cgtccgccgg ggatcgtctc gcagtgccga
     1681 aagtataccg ccgggccgtt ggagattcgc gtgccgacga acgccacgat cggcgcaccc
     1741 agaagagcca tacgccgtgt gccgctcgct ggcatggggt tgcaccggct gatacaatcc
     1801 cgtgttaata cacgaccgtg aaccggaaca gcgcgtgggg atcggaggag gtacccatga
     1861 catccgttcg gtacgggaaa gacccatatg catgacccgc acccgcgcga cacatgttgc
     1921 cgggtgcgtt ttgtttgagc cgtattcccc gcagcggcgt cgggcacgtg ctgaggtcac
     1981 ggtcctggtc cacttccact catcgttggg gtcgttcggg cccacccggg ctcggcacac
     2041 gcaaggagcg atggcccatg aactcaagca tgatcggcaa gatcgagaag gcccatcggt
     2101 acgcgcggga gccggagcgc atcacgttcg agacgttcac cgcctccttc cgcggcagtc
     2161 acgacgacta caccgtctcc ctgacagcaa acggatggga atgcacctgc cacaccttcg
     2221 agacgcacat gcttggaaca tgcagtcaca tcatggcgat ccagcacatc cttggcggga
     2281 tgctgcccga ccgggcacgc tatgactcgg cagaggccga gaccctcgag gccgtcgccg
     2341 gctagttgac cgcgaaccgg caatccgaga ggcgtcgatc atcctgacgc tgatggtccg
     2401 agcccggctc gatcggcagc gtcaggattc gtccatccgc ggtccgttca gcggcggcgc
     2461 gcaattcccc agcctcggcg atccgcacga tccgcgtctc cccgatcgcg ctaccggacg
     2521 cgggcacgcc aaactggcgc gtcaccagca tgacggcccc gccccacgtg gcggcgacgg
     2581 tacgccaact cactctcccg agggtcacgg ctgcccctcc tcgcgatgcc cggccgggaa
     2641 gatccggcga tagtcccgca ccggcaggat aaacaccaca gcgcctccaa cggtgacatc
     2701 gtgcacgccg atggggaacc actcgatgaa ctcctgctcc ccaggatcga tggccgattc
     2761 cacgcggggc tcgcagtgct cacggatgat ctccaggcac tgctggaccc gctcgtcgtc
     2821 cacgcccatg agaatcgtcg cgttggccat gcgcaggaag ccgccggtgc tcacgatctt
     2881 cgtcgcgccc aggccagccg aggtgacggc gcgcaggagc cgatcgctgt cgtacgcctg
     2941 aatgatcgcg atgatgagct tcatgcgtac aggaccctcg gcgatggcgt gtggcaggag
     3001 cgggcggccg gcgctggctt gtccgagcca gcgccatcat gc
//
LOCUS       DASYWL010000029         5757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_451654, whole genome shotgun sequence.
ACCESSION   DASYWL010000029 DASYWL010000000
VERSION     DASYWL010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451654"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..505)
                     /locus_tag="VGR22_01140"
     CDS             complement(140..505)
                     /locus_tag="VGR22_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127207.1"
                     /translation="MTVVPPPFFDGYEFSLKGSPRHYDDVRAIIEAGGEADVWFGEAL
                     AYERGAGVALWRIRAKSFGWLDQLYDWWAEAERVEPINVTFHLYLPDDPKYPVLDLRQ
                     YTPAEVVEFLRANAPYVRD"
     gene            681..1244
                     /locus_tag="VGR22_01145"
     CDS             681..1244
                     /locus_tag="VGR22_01145"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HEV2127208.1"
                     /translation="MPAPPPAPSSAPRRNLELKVRVSPKTLEHLRALASSIADGPSEQ
                     QHQDDRYFRVPEGRLKLRAITDDTGVSRAELIAYRRPDAAGSRWSAYRIAQLEAGQAQ
                     DLAETLGHVLRPLARVRKRREVLLHGATRIPLDQVEGLGAFVELETVVMHQNATSAEQ
                     EHRRVIAALGLDAASRGRLLQRPARIR"
     gene            1290..2600
                     /locus_tag="VGR22_01150"
     CDS             1290..2600
                     /locus_tag="VGR22_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2127209.1"
                     /translation="MSESTQLAAPVATNGRRSRARLQSVRRTYMVGDLRMSVLDWMGP
                     EQPLSPPVVLLHGALQSSEGMANLASHLARRGRVIVPDLRGRGATGQPEDGYDPKTMA
                     ADVAGLIAEMGVDRPVVIGRLHGGLVAWHLASNHPEAVSGLIIGDTAPEVTNGHAKRL
                     IEMTRLLPRSFRTIDDAYAFYQHTLGLPLERALHDIPFDLRRSRDGFTWRHNLDLVAR
                     IEEAALPRADWDVLAKIAVPTLVLRGQRGSLSPEMAERMCELIGDCTVQTILSSGRDV
                     FLGGGAEQAFAAIDLFLLRFTDTGPIRDVAVPDTLARSYIEPLVRGLNGKDAAALRSM
                     LTRDAVVEVVRDSQPVHKGSAETVNELMQTLLEQHPSAVFAVERFLASRREAAVRLVS
                     RSQPAGDGARDEEVSLYLDLWIEIDREGITFARLQLARVPDGEG"
     gene            complement(2892..3752)
                     /gene="uppP"
                     /locus_tag="VGR22_01155"
     CDS             complement(2892..3752)
                     /gene="uppP"
                     /locus_tag="VGR22_01155"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HEV2127210.1"
                     /translation="MEWWQAIILGIVQGLTEFLPISSSGHLIIFPWLFDWETPGLSFD
                     ASLHLGTLLAVVVYFSREILLMLRAIPVALSNPVGLLTGTAPDGPRHAEARLGLLIVV
                     ATIPGLIAGLLLESVVEEVFHTEDNSTLGISVIATMLIVVGIVLWVAERVGHRAREIS
                     GMRWADALVIGLAQALAIIPGTSRSGATITAGLFRGLERDDAARFSFLAGMPLILGAG
                     LKSLLDVLQEGTEGSDLFLYLLGGVSAAVVGFATIWGLLRFLQRQSTFVFTVYRVIFG
                     LFLFLMLAVQ"
     gene            complement(3882..4931)
                     /locus_tag="VGR22_01160"
     CDS             complement(3882..4931)
                     /locus_tag="VGR22_01160"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEV2127211.1"
                     /translation="MRSWRAWVGIAISTLFLWLAFRGQDLGEIRDALRQTNLWWVVPA
                     LLLYFTGVWVRAMRWQVLMRPLAPVSARDLFPVAVVGYMANNVLPLRAGELVRSVVIG
                     QRFGVRKTSTLATIAVERLFDGLTMLGFMLLATTVVSFTSELQNLAIIAFVLFAVLLI
                     GLFALTLGGSVVDRLLQLVLGPMPTALADRVERMAESFLSGLGIFRSRSDFLKVAGLS
                     VTAWLFEASTYWVLAIGFGGTMREIMGWAETLMTTGVANMATLIPSSPGYIGQFEYGV
                     KLVLNGALGVADGPALAYAILVHAVLYFPITLWGAWEWFRQHLSWKAIQESGEGNGRT
                     AEQPVLHSESPGGNA"
     gene            complement(5067..5267)
                     /gene="infA"
                     /locus_tag="VGR22_01165"
     CDS             complement(5067..5267)
                     /gene="infA"
                     /locus_tag="VGR22_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003808114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEV2127212.1"
                     /translation="MAVEGKVVEALPNAMFSVELENGHTVLGHLGGRMRKNYIRVLPG
                     DRVVVELSPYDLTRGRITFRHR"
BASE COUNT         1041 a         1955 c         1836 g          925 t
ORIGIN      
        1 atccgacacg gcgctggcga gcgcggtcaa tgtcctccgc tacgccccgc gcgttcaagt
       61 gggctgccgg gcggacacga aaaacgcggg agtgatggat tcactcccgc gtttgccatt
      121 tcggtcgaat tggtttccgc tagtcgcgaa cgtagggggc gttcgcgcgg aggaactcca
      181 ccacctcggc tggcgtgtac tggcgcaggt cgagcaccgg gtacttcggg tcgtccggca
      241 ggtagaggtg gaaagtcacg ttgatcggct cgacccgctc ggcctcggcc caccagtcgt
      301 acagctggtc cagccagcca aagctcttcg cgcgaatgcg ccacagcgcg accccggcgc
      361 ctcgctcgta ggcgagcgcc tcgccgaacc agacgtccgc ctcgccaccg gcctcgatga
      421 tcgcacggac gtcatcgtag tgccgtggcg atcccttgag tgagaactcg tagccatcga
      481 agaacggcgg cggcaccaca gtcatctgct tcctgagccg ggccttgcgc cctggcgaga
      541 acaaccccat atacggctcc tccgcacggt atagttgcgt atcgtatgcg agtgggtgtc
      601 ggaccccgca gccacacgcc gtgctgggac ccgtctggtt tcacgcggta ttgtagcgcg
      661 tcctggaacg cctcctgacc atgccggcac cacctcccgc cccttcgtcg gcgccccgcc
      721 gcaacctcga gcttaaggtc cgcgtctcgc cgaagacgct ggagcatctt cgcgcacttg
      781 cctcgagcat cgccgacgga ccgtccgaac agcagcatca ggacgaccgc tactttcgcg
      841 tgcccgaggg ccgattgaag ctgcgagcaa tcacagacga tactggagta tcgcgggccg
      901 aactgatcgc gtaccgccgg ccagatgcgg cgggatcccg atggagcgcc taccgcatcg
      961 cgcaactgga ggcggggcag gcgcaggatc tcgcggagac actcggacat gtcctgcggc
     1021 ccctcgcgcg ggttcgcaag cgacgcgaag tcctgctgca cggcgcgacc cgcatccccc
     1081 tcgaccaagt cgaggggctg ggcgcgttcg tggagctgga aacggtcgtg atgcatcaga
     1141 acgccacaag cgccgagcag gagcaccggc gagtgatcgc cgccttgggc ctggacgcgg
     1201 ccagtcgagg ccggctccta cagcgacctg ctcgaatacg ttgacgatga cgcgccggca
     1261 cggccggcgc ctgacagggg acaacaatga tgagcgaaag tacacaactg gcggcaccgg
     1321 tggccacgaa cgggcgccgc agccgcgcgc ggctgcaatc cgttcgccgg acctatatgg
     1381 tcggagacct gcgcatgagc gtgctcgact ggatgggtcc cgagcaaccc ctcagcccgc
     1441 cggtggtgct gctccacggc gcgctgcaga gctctgaagg catggccaac ctcgcatccc
     1501 acctcgcccg gcgcggacgg gtgatcgtgc cggatctccg ggggcgcggt gccaccggtc
     1561 agccggagga tgggtacgac ccaaagacca tggcggccga tgtcgccggc ctgatcgccg
     1621 agatgggagt cgaccggccg gtcgtcatcg gccggctcca tggcggcctc gtcgcgtggc
     1681 acctcgcctc gaaccacccg gaggcggtct ccggcctgat catcggcgac accgcgccag
     1741 aagtcaccaa cggccatgcc aagcgcctga tcgagatgac gcgcttgctg ccgcgctcat
     1801 ttcgcacgat cgacgacgct tacgccttct atcagcacac gctcgggctc ccgctggagc
     1861 gggcgctgca cgatatcccc ttcgatctgc gccgcagccg cgatggcttc acctggaggc
     1921 acaatctcga cctcgtcgcc cggatcgaag aggcagccct cccccgcgcc gactgggatg
     1981 tgctggcgaa gatcgcagtg cccaccctcg tgctgcgggg tcagcgcggg tcactctcgc
     2041 ccgagatggc cgagcgcatg tgcgagttga tcggtgactg caccgtgcag acgatcctct
     2101 cctccggccg ggatgtcttt ctcggcggcg gcgccgagca ggccttcgcg gcgatcgacc
     2161 tcttcctgct gcgcttcaca gacaccggac cgatccggga cgtggccgtg cccgacaccc
     2221 tggcccgcag ctacatcgag ccgctggtgc gtgggctcaa cggcaaggat gccgccgcac
     2281 tacggtcgat gctgacccgg gatgcggtgg tcgaggtggt gcgtgacagc caaccggtgc
     2341 acaagggatc ggcggagact gtcaatgagc tgatgcagac gctgctggag cagcacccgt
     2401 cggcggtctt cgcggtcgag cggttcctcg cctcacggcg tgaggcggcg gtgcggctgg
     2461 tctcccgctc gcaaccagca ggagacggcg cgcgggacga ggaggtctcg ctctacctcg
     2521 atctctggat cgagatcgac cgggagggaa tcaccttcgc ccgccttcag ctcgcacgcg
     2581 tcccggatgg cgagggatag gccgtttggg gtggagtagc agcggcaggg ccgcccctcc
     2641 gcaacacacc tcgccgtcgg gcgtcgcatc tcgaacgtgg accacaccgc gcgcagaaag
     2701 cggccttcgg cctgggcgga caacgcccgc cccaacagag tgacccctct tccttcgccg
     2761 ccgcgcgccg accgctggcc gcgacgggac ccgcccctcc gtgggcgggc cgacacgttg
     2821 gcacccgtat cgttggtcat gggattccga cgtcgacgga tgcgacgcaa cctgtgcatc
     2881 acactccggg gctactggac ggccagcatg aggaagagga acaggccgaa gatgacgcgg
     2941 tagacggtga agacaaaggt gctctggcgc tgcaggaagc gcagcaaccc ccagattgtt
     3001 gcgaacccga ctactgccgc ggagaccccg cccagcaggt agaggaacag gtcgctcccc
     3061 tccgtgccct cctgcaggac atcaagcaac gacttcagtc ccgcgccgag gatgagcggc
     3121 atgcccgcga ggaatgagaa ccgggccgcg tcgtcccgct ctaggcctcg gaagagcccg
     3181 gcggtgatcg ttgcgccgga gcgcgaggtg cccgggatga tcgccagtgc ctgtgctaaa
     3241 ccgatgacga gcgcgtcggc ccaccgcatg ccggagatct cccgcgcccg gtgcccgacc
     3301 cgctccgcca cccagaggac gataccgacg acgatcaaca tcgtcgcgat cacgctgatg
     3361 ccaagcgtgc tgttgtcctc ggtgtggaag acctcctcca ctaccgactc cagcagcagt
     3421 cctgcaatca ggccggggat cgttgccacc acgatcagca gcccgagccg tgcctcggcg
     3481 tgccgcgggc cgtccggcgc agtgcctgtg agaagaccaa ccgggttgct cagcgccacc
     3541 ggaatcgcgc gaagcatcag caggatctcg cgggagaagt acaccaccac cgcgagcagc
     3601 gtgccgaggt ggagcgatgc gtcgaagctg agcccgggcg tctcccagtc gaacaaccag
     3661 gggaagatga tgaggtgccc ggacgacgag atcggcagga actccgtcag cccctgtacg
     3721 atcccgagaa tgatcgcttg ccaccactcc atgcgtgaat cactcctgag cgtccggtct
     3781 gatcgtcacg tcacaacacg gccgggcctc gtgcccggcc gccgaacgtg ctgattgctg
     3841 gtttgaaggc ctgatcctac gtcaccgccc ggtgtcgggg ctcaggcgtt gccgccggga
     3901 ctttcggagt gcagcaccgg ctgctctgca gttctgccgt taccctcgcc actctcctgg
     3961 atcgccttcc agctcaagtg ctgacggaac cattcccagg caccccagag tgtgatcggg
     4021 aagtagagta ccgcatgcac cagaatcgcg taggcgagcg ccggcccatc cgccaccccc
     4081 agtgcgccgt taagcaccag cttgacgcca tactcgaatt gcccgatgta gccaggggat
     4141 gaggggatga gggtcgccat gttggcgacg cccgttgtca tgagcgtctc cgcccaaccc
     4201 atgatttccc gcatggtgcc gccgaagcca atcgccagca cccagtacgt cgacgcctcg
     4261 aacagccatg ccgtgaccga gagaccggcc actttcaaga aatcgctccg actacggaag
     4321 atgccgagac cgctgaggaa tgactccgcc atccgctcca cccgatcggc gagagccgtc
     4381 ggcatcggcc cgagcacgag ctggagcagg cggtcgacca cgctgccacc gagggtgagc
     4441 gcaaagagcc caatcagcag gacagcgaag agcacgaagg cgatgatcgc gaggttttgc
     4501 agctcactgg tgaaggaaac gaccgtggtc gccagcagca tgaacccgag catggtaaga
     4561 ccgtcgaaca ggcgctcgac ggcgatggtc gccaacgtgg aagtctttcg cacgccgaac
     4621 cgctgcccga tcaccaccga gcgaacaagc tctccggcgc ggagcggcag cacattgttg
     4681 gccatatagc caacaactgc cacaggaaag agatcgcggg cgctcacggg cgcgagtgga
     4741 cgcatgagca cctgccagcg catggcgcgc acccagacac cggtgaagta gagcagcagc
     4801 gcggggacaa cccaccagag gttggtttgc cggagggcat cccgaatctc gccgagatcc
     4861 tgtccgcgaa aggccagcca gaggaagagg gtgctgatgg cgataccgac ccacgcgcgc
     4921 caggatctca tgaagcctcg ctctgggacc gcgagcacgc cgaagcgccg ggcgccggtc
     4981 gtcgcagccc gatggtctcg gatagcacca acacagaaag gccgggacct gtaggcccga
     5041 ccttgtcatg gtgcatggac tgtgtgctac cggtgacgaa aagtgattcg gccacgcgtg
     5101 aggtcgtatg gcgacagctc gacgaccacg cggtcgcccg ggagcacgcg gatgtagttc
     5161 ttgcgcatgc ggccaccgag gtgcccgagg acggtatgtc cgttctcgag ttccaccgaa
     5221 aacatcgcgt tcggcagcgc ctcgacgacc ttgccctcaa cggcgatggc gtcaggattg
     5281 gtgcgacgcg ggccctccga cgtcgcggtg ttcgggccct tgccctgctt gccctttggt
     5341 gtctgcggtt tcctcgccaa tatgctgtcc tctcctgtgt gatgtcctgc gattgcagcc
     5401 gtccgctcgc cgatatcttt ggcgaaggca tcaaaagcga atccgcaggt attgctcgtc
     5461 caatcccgct ctctatttta ccgcatccct gcaagtctcc tgccatcagc cgcaccgctt
     5521 tcaactttca accaaggtat ggttggccct accgtcgatc cgtcgctgct ttcgtatcct
     5581 gaacgcaggc ccgtgccggc tggcacgggc gaaacaacga cgaccgaaag gatacgatct
     5641 ccatgaacac actgggttgc gggttgtccc tcgttgcggg cgtgttcctg ctcatcgggc
     5701 tcttcccgtt cctcggctgg ctcaactggc tcgccacgct gccgctctcg ctgcttg
//
LOCUS       DASYWL010000030         3060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_207590, whole genome shotgun sequence.
ACCESSION   DASYWL010000030 DASYWL010000000
VERSION     DASYWL010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207590"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..220)
                     /locus_tag="VGR22_01170"
     tRNA            complement(144..220)
                     /locus_tag="VGR22_01170"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(184..186),aa:His,seq:gtg)
     gene            complement(647..733)
                     /locus_tag="VGR22_01175"
     tRNA            complement(647..733)
                     /locus_tag="VGR22_01175"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(697..699),aa:Leu,seq:cag)
     gene            complement(758..832)
                     /locus_tag="VGR22_01180"
     tRNA            complement(758..832)
                     /locus_tag="VGR22_01180"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(798..800),aa:Thr,seq:cgt)
     gene            1039..1650
                     /locus_tag="VGR22_01185"
     CDS             1039..1650
                     /locus_tag="VGR22_01185"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2127213.1"
                     /translation="MGTSRMMKIGFITLAMLFTCSLLAGSLVLIPFDELFGLNDADDV
                     QNIVDRNDELIEELEATVEANPDDVENVLLLANVLGNSGRLDDAVSYYEQAIELAPND
                     SSVRLDFARALADGGYRADAELQFERVLEMRPDSQEAQYYLAELYMSWDPPREEQARE
                     LYERAVESDPISFLAEQARNQLASFSGTSASPVATPAADGMGG"
     gene            1738..2799
                     /gene="ruvB"
                     /locus_tag="VGR22_01190"
     CDS             1738..2799
                     /gene="ruvB"
                     /locus_tag="VGR22_01190"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEV2127214.1"
                     /translation="MSEKSQQSRMMSGKNREEDARLDRSLRPRSLDEYIGQDRVKNNL
                     RIFIQAARAREEALDHILLYGPPGLGKTTLSSIIATEMGVNLRVTSGPAIERQGDLVS
                     ILTNLKQGDVLFIDEIHRLNRVVEEVLYPAMEDYAVDIVIGKGPSARTMRINLPRFTL
                     VGATTRLALMTGPLRDRFGAVLRLDYYDDDAMEQVIRRSAQVLDVEIKDDGARALATR
                     SRGTPRIANRLLKRVRDVAQVRGAGAVTDEIANSALDLLEVDQLGLDEVDRRILLALI
                     KKFQGGPVGIDTLAAATSEESDTIMDVYEPYLIQLGFLQRTPRGRVATRLAYEHLGIE
                     IPEAIEAFSAQQALFSTDE"
     gene            2796..>3060
                     /locus_tag="VGR22_01195"
     CDS             2796..>3060
                     /locus_tag="VGR22_01195"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEV2127215.1"
                     /translation="MSDERNEQSHIRMADYDYDLPGELIAQVPLEERAASRLLVLERG
                     SETITHSRFACLTDLLRPRDLLVFNDSRVVPARLRVRRETGGVG"
BASE COUNT          539 a          996 c          983 g          542 t
ORIGIN      
        1 catgtgtccc gaggtgcgac gacaaaccga ccccaacgcg gccccgccgc accggcatgg
       61 acacattagg gacacatttc gcgtcggaca cacgatcggg caccccgtcg gtgcccgatt
      121 ccgttgctat gactggtgcc tggtggggtg cctaacggga cttgaacccg taacctcttg
      181 ttccacagac aagcgctcta accgattgag ctataggcac catggcgatc aacggagaga
      241 aagggggtaa gccaggttct gttctcggat ggtcatctct ctcgacgaat gtcggtcacc
      301 tcgtcgcctc ggtgacggct ctcgacttcc ggcctcggcg agcaacctgc tggcggccga
      361 atcggagatt gctgcgggga ggattgccct tttcactctg cttgcgcagc cttggtctct
      421 gttgctctca cgcgccggtt ggcgcgtgcc gatctggact gtcaacggaa catcattccg
      481 ctgccctggc tttcgccagg caccctcgct ctttgcagcc cggactttcc tctggccctc
      541 gcggggccag cgaccatcac cccagacttc cgatgatctc ttctcatgcc aacgtgctgc
      601 gtccgaccca ccacatttcc gttggtcgag tcggccgccg ttgtcatggt gccgaagggg
      661 agatttgaac tcccacgggg ttgcccccac tacgccctga acgtagcgcg tctgccggtt
      721 ccgccacttc ggcgcgtgcg gatgctccac gagcttgtgg agccgaccgt gggattcgaa
      781 cccacgacct gctgtttacg aaacagctgc tctaccactg agctaggtcg gcgtccgctt
      841 cccgcggcgc agcgttacgt agtatagcaa aggtggggag ccacgccaag tgcggtggcg
      901 ggtggtatcg ttgccccggc acctctgccg atacccgcca gagtaccctc aggagtgacc
      961 agcttggccc gttcccgacc cgttcgccgc cccccggacc gaaggcgccc cgctcagagg
     1021 aagcccgcca cggagcagtt gggcaccagc cgcatgatga agatcggatt catcacgctg
     1081 gcgatgctgt tcacctgctc gttgctcgcc ggatcgctcg tcctgattcc attcgacgag
     1141 ctcttcgggc tgaacgacgc cgacgacgtg cagaatatcg tcgaccgcaa cgacgagctg
     1201 atcgaggagc tggaggcgac ggtcgaggcc aatccggacg acgtcgaaaa cgtgctgctg
     1261 ctcgccaacg tgctcggcaa ttcaggacgg ctcgacgacg ctgtctcgta ctacgagcag
     1321 gcaatcgaac tggcaccgaa cgactcctcg gtgcggctcg acttcgcccg ggcgctcgcc
     1381 gatggcggct accgggccga cgcggagctc cagttcgaac gagtgctgga gatgcgtcct
     1441 gacagccagg aggcgcaata ctacctcgcc gagctctaca tgagctggga cccgccccga
     1501 gaggagcagg ccagggaact gtacgagcgg gcagtcgagt cagacccgat ttccttcctc
     1561 gcggagcagg cgcgcaatca gcttgcgtcg ttctcaggca catcggcgtc gccggtcgcg
     1621 acacccgcgg ccgacggcat gggtggatga tacgaacagg cgttctattc gcgctatact
     1681 tgcgggagag catgacgggc ctggttggtc ccacggagca gagaaggtcg ccgcgccatg
     1741 tcggagaagt cgcagcagtc gcgcatgatg agcggaaaga accgggagga ggatgcccgg
     1801 ctcgatcgct cgctgcgccc gcggagcctc gatgagtaca tcgggcagga tcgggtcaag
     1861 aacaatctca ggatcttcat ccaggcggcg cgagcgcggg aagaggcgct cgatcacatc
     1921 ctgctctacg gcccgccggg cctcggcaag acgacgctct catcgatcat cgccaccgag
     1981 atgggtgtca atctccgcgt gacctccggt ccggcaattg agcggcaggg tgacctcgtc
     2041 tcgatcctga ccaacctcaa gcagggcgac gtgctgttca tcgacgagat ccaccggctc
     2101 aaccgagtgg tcgaggaggt gctatacccg gcgatggagg actacgcggt cgacatcgtg
     2161 atcggcaagg gcccctcggc gcgtacgatg cggatcaacc tcccgcgatt cacgctcgtc
     2221 ggtgcaacca cgcggctcgc gctgatgacg ggcccgctgc gggaccgttt tggcgcggtg
     2281 cttcgcctcg actactacga cgacgacgcg atggagcagg tcatccggcg gtcggcgcag
     2341 gtcctcgatg tcgagatcaa ggatgatggc gctcgcgcgc tcgccacgcg gtcgcggggc
     2401 acgccgcgca ttgcgaaccg gctgctcaag cgcgtccggg acgtggcgca ggtgcgaggt
     2461 gccggtgcgg taaccgacga gatcgccaac tcggcgctcg acctgctgga ggtcgatcag
     2521 ctcggcttgg acgaggttga ccgccggatc ctgctggccc tgatcaagaa gttccagggc
     2581 ggtccggtcg ggatcgacac cctcgccgct gcgacgagcg aggagtcgga cacgatcatg
     2641 gatgtctacg agccctatct catccagctc ggctttctcc agcgcacccc gcgcggccga
     2701 gtcgcgacgc ggctcgccta cgagcacctc ggcatcgaga ttccggaggc gatcgaggcg
     2761 ttctcggcgc agcaggcgct gttcagcacc gacgaatgag tgacgagcga aacgagcagt
     2821 cccatatccg catggcggac tacgactacg acctgccggg agagttgatc gcgcaggttc
     2881 cgctcgagga gcgggccgcc agtcgcctgc tggtgctcga gcgagggtcc gagactatca
     2941 ctcacagtcg cttcgcctgc ctgacagacc tcctgcgccc gcgtgatctg ctcgtcttca
     3001 acgacagtcg ggtggtcccg gcccgattgc gagtccggcg cgagaccggc ggggtggggg
//
LOCUS       DASYWL010000031         2500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_432846, whole genome shotgun sequence.
ACCESSION   DASYWL010000031 DASYWL010000000
VERSION     DASYWL010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432846"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VGR22_01200"
     CDS             complement(<1..426)
                     /locus_tag="VGR22_01200"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEV2127216.1"
                     /translation="MAEITNTADALEAIRKVRQVRQYEPGSVSDEDLNRILEIARWSG
                     SSRNTQPWRFVIVEDKDVLARLAELRPNITWVANGALAIALVFPGENDIHEAYDEGRV
                     SERIMIAAEMLGLGAGTAWFAENEGVAKQILGVPDDMVLH"
     gene            complement(573..1397)
                     /locus_tag="VGR22_01205"
     CDS             complement(573..1397)
                     /locus_tag="VGR22_01205"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV2127217.1"
                     /translation="MNAAIPSIAQVRRNARLYLTLQLVQLRAAVEYSGDFWIGIIGAM
                     LQQAAGLVFIAALFTQIPEVGGWTAWNIAILYGLAMIPKGLTEFLCDGPWMLRTQVNT
                     GEFDRVLVRPISPALQSATAIASIHGFGQLILGVVVLWAGMSRSEMTFTWWTLPFLLV
                     TIAASAVMIGALNYVINMIGFWEPSAQSALPTLVALMIDFAKFPLDVFNLVIRGLVTV
                     VIPYAFISYFPALLLLDRDAPWRWLGLATPLVAALVVAFTAWLWSKALNRYQGVGH"
     gene            complement(1401..2219)
                     /locus_tag="VGR22_01210"
     CDS             complement(1401..2219)
                     /locus_tag="VGR22_01210"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV2127218.1"
                     /translation="MNESARTAPWPKYLSIAMTSFQQAIAYRVTTILNIAITFIWVFI
                     LYYLWRAAFSERNLIAGYTWADMRTYVLLAYGINALVGWRVGSVMMGAIRTGEILREL
                     VRPLNYCVTQLALAAGFTVTEGFISLIFTLVLGIFVLDIRAPASSEMALLFALSVLVG
                     FVTKALIVFTVSLLTFWTLNGVGLMWAQQAVIAILAGTLVPIDLLPGWLRVAAEVLPM
                     RGIVYTPITLYLGKAEGWTVVQMLGMQLAWLVALWVFANWAWRAAFDAVEIQGG"
     gene            complement(2216..>2500)
                     /locus_tag="VGR22_01215"
     CDS             complement(2216..>2500)
                     /locus_tag="VGR22_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127219.1"
                     /translation="QRLHLTFGEEAPDANTNAESALAKHPHARIEQPDGTHLTITFDS
                     REATSGELIRDLATALPIADIRIEEPSAESIIRKLYEGSLHFDDAVVTAP"
BASE COUNT          463 a          745 c          889 g          403 t
ORIGIN      
        1 gtggagcacc atgtcgtccg ggaccccgag gatctgcttg gcgacgccct cgttctcggc
       61 aaaccacgca gtgccggcac cgaggccgag catctcggcg gcgatcatga tgcgctcgct
      121 gacacggccc tcgtcgtagg cctcgtggat gtcgttctcg ccggggaaga cgagggcgat
      181 cgcgagggca ccattggcga cccaggtgat gttcggtcgc aattccgcga ggcgcgcgag
      241 cacgtccttg tcctcgacaa tgacgaagcg ccacggttgg gtgttgcgcg agctgcccga
      301 ccagcgagcg atctcgagaa tcctgttcag gtcctcatcg ctgacgctgc ccggctcgta
      361 ctggcggacc tggcgaacct tgcggatcgc ctctagggca tcggcggtgt tggtaatctc
      421 ggccatcgtg ttgcctcctg tcatggtcgg tgtggtcggc ctgtccctgc atcatagcgt
      481 catgggacga gacggcaaat tcccggccac ggctcatgcc acgtacggga ggcaaccagg
      541 gagtcgacat cgggtgaact gttgggaccc cgctaatgcc cgacgccctg atagcggttg
      601 agggccttgc tccacagcca agcggtgaag gcgacgacga gcgcggcgac cagcggcgtg
      661 gcgaggccga gccagcgcca tggggcgtcg cggtcgagga gcaggagggc ggggaagtag
      721 ctgatgaagg cgtaggggat caccacggtg acgaggccgc ggatcaccag attgaagacg
      781 tcgagcggga acttcgcgaa gtcgatcatg agcgcgacca gcgtgggcag cgcgctctgg
      841 gccgatggct cccagaagcc gatcatgttg atgacgtagt tgagcgcacc gatcatcacc
      901 gcgctcgcgg cgatcgtcac cagcaggaag ggcagcgtcc accaagtgaa cgtcatctcg
      961 gaacgactca taccggccca cagcacgaca acaccgagga tgagttgtcc aaacccgtgg
     1021 atggaggcga tcgccgtggc ggactgcaac gccggggaga tggggcggac cagcacgcgg
     1081 tcgaactcgc cggtgtttac ctgcgttcgc agcatccatg gtccgtcgca gagaaactcg
     1141 gtcagccctt tcgggatcat cgccaagccg tagaggattg cgatattcca agcggtccag
     1201 cccccgactt cggggatctg ggtgaagagg gcggcgatga agacgagtcc ggctgcctgc
     1261 tgcagcatcg cgccgatgat gccgatccag aagtcgccac tgtactcaac cgcggcccga
     1321 agctggacca gctggagggt gaggtagagc cgcgcgtttc tgcgtacctg cgcgatcgac
     1381 gggatggcgg cgttcaccgg tcaccctccc tggatctcga cggcgtcgaa ggccgcgcgc
     1441 cacgcccagt tggcgaagac ccacagcgcc accaaccagg cgagctgcat gccaagcatc
     1501 tggacgaccg tccatccctc ggccttgccg aggtagaggg tgatcggtgt gtagacgatg
     1561 ccgcgcatgg gcagcacctc ggcggcgacc cgcagccagc ccggcaggag gtcgatcgga
     1621 acgagcgtgc cggcgaggat ggcgatgacc gcctgctggg cccacatgag cccaacgccg
     1681 ttgagcgtcc agaaggtgag cagcgagacg gtgaagacga tcagcgcctt ggtgacgaag
     1741 ccgacaagga cgctgagggc gaagagcagg gccatctccg acgaggcggg agcccggata
     1801 tcgagcacga agatgccgag cacgagcgtg aagatgaggc tgatgaatcc ctcggtgacg
     1861 gtgaagccgg cggccagggc gagctgggtg acgcagtagt tgagcgggcg gaccagctcg
     1921 cgcaggatct ctccggtgcg gatcgcgccc atcatcaccg agccaacgcg ccagccgacg
     1981 agcgcgttga tgccgtaggc gagcagcacg taggtgcgca tgtcggccca ggtgtagcca
     2041 gcgatcaggt ttcgctcgga gaaggcggcg cgccacaggt agtagaggat gaagacccag
     2101 atgaacgtga tggcgatgtt gaggatcgtg gtgacgcggt aggcgatcgc ctgctggaac
     2161 gatgtcatgg cgatcgagag gtacttcggc cacggcgcgg tccgggcgct ctcgttcatg
     2221 gcgccgtcac caccgcgtcg tcgaagtgga gcgagccctc gtagagcttg cggatgatcg
     2281 actccgcgct cggctcctcg atgcggatat cggcaatcgg cagggcggtt gcgaggtcgc
     2341 gaatcagctc gccggaggtc gcctcgcggc tgtcgaaggt gatcgtcaag tgggttccgt
     2401 ccggctgctc gatgcgcgcg tgtgggtgtt tcgcgagggc tgattccgcg ttggtgttgg
     2461 cgtccggagc ctcctcgccg aaggtcaggt gcaggcgctg
//
LOCUS       DASYWL010000032         4007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_432905, whole genome shotgun sequence.
ACCESSION   DASYWL010000032 DASYWL010000000
VERSION     DASYWL010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432905"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /gene="cofD"
                     /locus_tag="VGR22_01220"
     CDS             <1..846
                     /gene="cofD"
                     /locus_tag="VGR22_01220"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEV2127220.1"
                     /translation="IVNTADDFELYGLHISPDLDTVMYTLAGIANPATGWGIEGDTRA
                     TLDAIARYGDDPWFLVGDQDFATHILRTHALHEGRMLTDVTLRLASSLGVRMPILPMT
                     DDRVATEVRTADGWLAFQDYFVARRHADEVLDVRFAGIDSARPSDRVIMAISEAELIL
                     FCPSNPLVSIEPILALSGVRDAIMASPAPVVAVSPIVGGKALKGPADRMLASKGVEVS
                     ARGVARYYEGLIDGIVIDAEDAAFADDIRGAGLAVDVRNTIMGGRDDRMRLAREILDV
                     EWVPR"
     gene            843..1580
                     /gene="cofC"
                     /locus_tag="VGR22_01225"
     CDS             843..1580
                     /gene="cofC"
                     /locus_tag="VGR22_01225"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEV2127221.1"
                     /translation="MSRTLAVIPVRGAMRTKTRLAPLFGAGARVCLVRHMLLHVLDAI
                     EQSGVVDHTLIVTRDPDAVPDQITESPSCTVAAQPGEGLNAAIELGRDRAVAGGYDQV
                     LILLPDLPMLVPGDVGALLAESAPVVIAADRHGTGTNALLLRLCGPGERLQFAFGPRS
                     AARHIAEIDRLGVRWTMVDTSGLRHDLDTPDDWQVFPEAVHRRLLECIVEPTAGGTSG
                     GVDDDEVDECYAARAVSPSCHARASNG"
     gene            complement(1593..2456)
                     /gene="prmC"
                     /locus_tag="VGR22_01230"
     CDS             complement(1593..2456)
                     /gene="prmC"
                     /locus_tag="VGR22_01230"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEV2127222.1"
                     /translation="MTSHADLIAEATERLQSTSTSARLDAEVLLRHVTGLDRTGLFLA
                     LRETASADTQERFRSLVDRRSAGAPIAYLTGTREFMGMPFAVTPDVLVPRPETELLVE
                     WALAMLPRFDHRPLRVADVGAGSGAIAVSVAALAEVPVTMTAVEPSAGAREVIARNAD
                     SLLPPERRLRFRITDDDLLATTVGPFKLVLANLPYLTPAQIAENPDLAAEPRLALDGG
                     PDGLDLIKRLIKQLPERAAPSCATGLEVDPSQARRVRALLEAALPDAEVSSIRDYAGF
                     ERHIVATRLKD"
     gene            complement(2821..3921)
                     /gene="prfA"
                     /locus_tag="VGR22_01235"
     CDS             complement(2821..3921)
                     /gene="prfA"
                     /locus_tag="VGR22_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEV2127223.1"
                     /translation="MQSISVNLNELERRYTELGHLMADPAVATDPERLMALGKERSDL
                     EAIVGAWREFRTNEEEIAEARLMTEDCDREIAELAHEELRELRRRQAELEGNIQRLLV
                     PKDPNDEKDVIVEIRAGTGGDEAALFAADLFRMYTRYAERQRWKVDVMSATETDGGGF
                     REIIFEVRGDGAYSYLRYESGVHRVQRVPQTESQGRIHTSTASVAVLPEAEEVDLQIN
                     ENDLRIDVFRSSGHGGQSVNTTDSAVRITHVPSGIVVSMQDEKSQLKNKTKAMSVLRS
                     RLYEAEQQRLAEERGDVRRSQIGSGERSEKIRTYNFPQDRITDHRIKKSVSNIPAVLN
                     GELDELIQDLRAADEAERLAEAGLAQDASAAV"
BASE COUNT          613 a         1341 c         1318 g          735 t
ORIGIN      
        1 atcgtcaaca ccgccgacga cttcgaactc tacggattgc atatcagccc ggatctcgac
       61 accgtcatgt acaccctcgc cgggatcgcc aaccctgcga ccggctgggg catcgagggc
      121 gacacccgcg cgacgctcga cgcgatcgcc cggtacggcg atgatccctg gtttctcgtg
      181 ggcgatcagg atttcgccac acacatcctg cgcacgcatg ccctccatga gggccgtatg
      241 ctcacggatg tcaccctccg gctcgcttcc agccttgggg tccgcatgcc catcctgcca
      301 atgaccgacg accgtgtggc cacggaggtg cgtaccgccg atggctggct ggcgtttcag
      361 gattacttcg tggcgcggag gcacgctgac gaggtcctgg atgtgcgctt cgccggcatc
      421 gacagtgcca gaccatcgga tcgggtgatc atggctattt ccgaggcgga gttgattctc
      481 ttctgtccgt ccaaccccct ggtgagcatc gagccgattc tcgccctctc cggcgtacga
      541 gatgcgatca tggcgtcacc ggcgccggtg gttgccgtca gcccgatcgt gggcggcaag
      601 gcgctcaagg gaccagccga ccggatgctc gcctcgaagg gggtggaggt ctctgcccga
      661 ggtgtggctc ggtactacga ggggctcatc gacgggatcg tgatcgacgc cgaagatgcg
      721 gcattcgcag acgacatccg tggagccgga ctcgccgtgg acgtgcggaa cacgatcatg
      781 ggcggtcgcg atgatcgcat gcggctcgcc cgggagattc tcgatgtgga gtgggtgccc
      841 cggtgagtcg cacgctcgcg gtgatcccgg tccggggtgc catgcgcacc aagactcggc
      901 tggcaccgct cttcggtgcc ggcgctcgtg tctgcttggt gcggcacatg ctcctgcacg
      961 tgctcgatgc catcgagcaa tccggagtcg tcgatcacac gctcatagtc acccgcgatc
     1021 ccgacgcggt gcccgaccag atcacagaat cgccctcatg caccgtggca gcacagccgg
     1081 gcgaggggct caacgccgct atcgagctgg gacgggaccg ggcggtcgcc ggcgggtacg
     1141 atcaggtgct gatattgctg cccgacctgc cgatgctggt accgggcgat gtgggggcgc
     1201 tgcttgcgga atccgccccg gtggtcatcg cggccgaccg tcatggcacc ggcacgaatg
     1261 ccctgctgct gcggctctgc ggtcccggtg aacgtctgca gttcgccttc ggtccccgga
     1321 gcgcggcgcg tcacatcgcg gagatcgacc gtctgggggt gaggtggacg atggtcgaca
     1381 cctctgggct ccggcacgat ctcgataccc ccgacgactg gcaggtgttc cccgaagcgg
     1441 tccatcggag gcttctggaa tgtattgtgg agccaactgc cggaggcacc agcggagggg
     1501 tcgacgacga tgaggttgac gaatgctacg ccgcgcgtgc cgtctccccg tcatgtcatg
     1561 ctcgggccag caatggttga ccgcaagtcg gatcagtcct tgagcctcgt tgcgacgatg
     1621 tgccgctcga agccggcgta gtcccggatg ctggatacct cggcgtcggg cagcgctgct
     1681 tccagcagcg cccggactcg tctcgcctgc gacgggtcga cctccagccc ggtcgcgcat
     1741 gacggtgccg cccgctccgg taattgcttg atcagccgct tgatcaggtc gagcccgtct
     1801 ggcccgccgt ccagcgccaa acgcggctcg gcagcgagat ccggattctc cgcgatctgc
     1861 gctggcgtga gataggggag gttggcgagc acgagcttga agggacccac cgtggttgcc
     1921 agcaggtcgt cgtcggtaat ccggaatcgc agtcgccgct ccggcgggag gagcgagtcg
     1981 gcgttgcggg ctatcacctc ccgtgcgccc gccgacggct caacggccgt catcgtcact
     2041 ggcacctctg ccagcgccgc gaccgatacc gcgatcgcgc cggacccggc accgacatcg
     2101 gccacccgca gcggccggtg gtcgaaccgt ggcagcatcg ccagcgccca ttcgaccagg
     2161 agctccgttt cgggacgggg cactagcaca tccggtgtca ccgcgaacgg catccccatg
     2221 aactcgcgcg tgccggtgag ataggcgatc ggcgcacccg ccgaacgtcg atcgacgagc
     2281 gatcggaacc gctcctgtgt gtcggcgctg gcggtttccc tgagtgcgag gaagagccct
     2341 gtgcggtcga gaccagtgac gtgacgcagc agcacctcgg cgtcgagccg ggcgctcgtg
     2401 ctggtgcttt ggagacgctc ggtcgcctcg gcaatgaggt cggcgtggga ggtcagcgga
     2461 tcgggccttt ctgcacgata ccggctcgga agacgctacg atagacgcaa cgacactatt
     2521 tcgctcaggc catgatagtc cctgcggggc cagcgacctg caaaggctgt ttgcggtggc
     2581 agcggcggcg cgactcgtgt cttcgctgtt gagcctgggt agcagaggca catggaaaga
     2641 cgctttgtac cccatgccgc gagcgcgcgg gagccggtgt gggcgtcaac aaacctttgc
     2701 ctgcccatcc tgacgactac gctcacgacg acccggggtg ggcctgctgc gatgccgcag
     2761 cctctcgaaa cggaaacccg cccatttgca gggcgggcct acctggtcgt ccgaatcttc
     2821 ctacaccgcc gcggaggcgt cctgggccag gccggcctcc gccaggcgct cggcctcgtc
     2881 ggcggcgcga agatcctgga tcagctcatc cagttccccg ttgagcacgg ctggaatgtt
     2941 gctgacgctc ttcttgatgc gatggtcggt gatgcggtcc tgcgggaagt tgtaggtgcg
     3001 gatcttctcg ctgcgctcgc cggagccgat ttgcgatcgc cgcacgtcgc cccgctcctc
     3061 ggcgagccgc tgctgctcgg cctcgtagag ccgcgatcgc agcacgctca tcgccttggt
     3121 cttgttcttc agctgcgact tctcgtcctg catgctcacc acaatgccgg acgggacgtg
     3181 cgtgatgcgc accgccgagt cggtcgtgtt gacgctctgg ccgccgtgcc ccgaggagcg
     3241 gaaaacgtcg atgcggaggt cgttctcgtt gatctgcagg tcgacttcct cggcctccgg
     3301 cagcacagcg acactggccg tgctggtgtg gatgcgcccc tggctctccg tctgtggcac
     3361 gcgctgcacg cggtgcacgc cggattcgta tcgcaggtag ctgtaggcgc cgtcgccacg
     3421 gacctcgaag atgatctccc ggaacccgcc gccgtcggtc tcggtggccg acatgacatc
     3481 gaccttccac cgctggcgct cggcgtaccg cgtgtacatg cggaagaggt cggccgcgaa
     3541 gagcgccgcc tcgtcgccgc ctgtaccggc acggatctcg acgatgacgt ccttttcgtc
     3601 gttggggtcc ttcggcacga gcagccgctg gatgttgcct tccagttccg cctgccgcct
     3661 gcgcagctcg cgcaactcct cgtgggcgag ctcggcgatc tcccgatcac agtcctcggt
     3721 catcaggcgc gcctcggcaa tctcctcctc attcgtgcgg aactcccgcc atgcccccac
     3781 gatcgcctcg agatcggacc gctccttgcc gagcgccatc aggcgctcag gatcggtcgc
     3841 caccgccggg tcggccataa gatggccgag ctcggtgtac cgccgctcca gctcattgag
     3901 atttacgctg atactctgca tcgtggcagc catgtgggat gcttctccgc gaggaaacga
     3961 ggcctgctgg gtccgggacc gcaactcctg accctcaagt atagccc
//
LOCUS       DASYWL010000033         5574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_507931, whole genome shotgun sequence.
ACCESSION   DASYWL010000033 DASYWL010000000
VERSION     DASYWL010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5574
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507931"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VGR22_01240"
     CDS             <1..195
                     /locus_tag="VGR22_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127224.1"
                     /translation="GLPQEDCYVNVLRDAMAVDTLGACGIYVGTTGGQVYVSPDSGDT
                     WHATASNLPKVLSVEVQTLP"
     gene            192..491
                     /locus_tag="VGR22_01245"
     CDS             192..491
                     /locus_tag="VGR22_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEV2127225.1"
                     /translation="MIRVILPAHLRTLARTEHEVSLDNVAAPVTARSIVDALEATYPM
                     LKGTIRDHQTKQRRPMVRFFACEQDLSHDPIDDPLPEAVATGKEPFWIVGAMSGG"
     gene            complement(749..922)
                     /locus_tag="VGR22_01250"
     CDS             complement(749..922)
                     /locus_tag="VGR22_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127226.1"
                     /translation="MKTLRKLFTRKNANGARRFDRYYGQVVHAGSGYPTADEARRDMR
                     DHDRMLIGFGLPR"
     gene            complement(1111..2436)
                     /locus_tag="VGR22_01255"
     CDS             complement(1111..2436)
                     /locus_tag="VGR22_01255"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEV2127227.1"
                     /translation="MVTIETHEQALAAIHERSGYDRGFISNPFAGDDAARLGLKRTQA
                     MLDRLGNPERSYKIVHVAGSKGKGSTCVFIDAILRATGRRTGRYLSPHLHSWHERFVV
                     DDRSITPEAFTTLTADSIAAAEAIETASPELGEVTAFELATAMALLHFRQEGCEVAVV
                     EVGLGGTLDATNVVDPAVSVITPLDYEHTAILGDTMAEIAANKAGIIKPGRPVVSAAQ
                     PSDGMHVIERRATELGSPLLVAGRDWRTTGTDRAFGAEGPWGTFRELATALPGQHQVD
                     NSGLAIAATYLVDPTTTEAVVRAGLLGAHLPGRFEVVPLPDGRAAVLDGAHTPAATRA
                     LAAALRDRFPNQSVTVVVGMLRDKDPGPVITPLLDVADRWIAAAPNSPRALPAETVAA
                     AIEALGGRCEVAADVASGIGAAREYAESVIFVTGSLTTVAEGRVALGLG"
     gene            complement(2525..3535)
                     /locus_tag="VGR22_01260"
     CDS             complement(2525..3535)
                     /locus_tag="VGR22_01260"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HEV2127228.1"
                     /translation="MKARKTPFTRRNFVGSLGATALAAPVALAGQRTMASEGHGDDDG
                     ELTVDEMDEMHREGIQSFPAPTEGLGNQPLEYTMDGDVKVFELTASVFDWEFRPGEYA
                     EAWGYNGAVPGPEIRVTEGDKVRVHLTNELPESTAIHWHGLVVPVKMDGVPFITQPPV
                     KPGETFTYEFTIREGNSGSHMYHSHHNSAFQVTQGLLGAFIVEPKDPSNEPEYDKEYT
                     LILNDGPIGGFSINGKSFPATEPLTAKQGEKIRVRYMNEGLMIHPMHLHGMPMTVTHK
                     DGYALPQPFVCDTLNIAPGERWDAIVEATEPGTWAFHCHILSHAESKHGMFGMVTALI
                     VE"
     gene            3879..4415
                     /locus_tag="VGR22_01265"
     CDS             3879..4415
                     /locus_tag="VGR22_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127229.1"
                     /translation="MARRSTLPQRSRSANLLSTVLFIAAIGFAAAAIWVWYTDDSESR
                     PGEPPPAERIEDIDLAHVLSVLKERNDDWTYSRNPATASTDQFNAPGQALQLDDALLF
                     VFIFTGAGAEERIAAREAASERVDLETMTLTTPSGTVINDNGEPLWMAEHSNVMTILV
                     GGDQALANEVAEALTDLP"
     gene            4441..5148
                     /locus_tag="VGR22_01270"
     CDS             4441..5148
                     /locus_tag="VGR22_01270"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEV2127230.1"
                     /translation="MTDSTRPDANAIRPPAPSAGEPVPTLADDDTDPDREQQGRIGYG
                     RWGRLSPIGLALLILVTLGVIWVMDQDNADDETDGLPSSTTTGELQDPRPAPEFELAL
                     MNGEGTVSLEELRGNTVVVNFWASWCAPCREEMPALQESAEAHAGDEVVFVGVGAKND
                     KQDAAEAFVEEFGITYPIGRDTEGGDSLSGQIEKAFGVPGYPATFFITPEGMINQIVF
                     GPLEIEDLDTYITNTTD"
BASE COUNT          987 a         1774 c         1836 g          977 t
ORIGIN      
        1 ggcctgccgc aggaggattg ctacgtcaat gtgctgcgcg acgcgatggc ggtcgacacg
       61 ctcggcgcct gtggcatcta cgtcgggacc accggcgggc aggtctatgt ctcgccggac
      121 agtggcgaca cctggcatgc caccgcgagc aacctgccga aggtgctctc cgtggaggtg
      181 caaaccctgc catgatccgc gtcattcttc cggcccacct gcgcacactg gcacgcactg
      241 agcacgaggt gtcactcgac aacgtcgccg caccggtgac agcacgcagc atcgtcgacg
      301 cgctggaggc cacctacccg atgctgaaag gcacgatccg cgaccatcag acaaagcagc
      361 gccgcccgat ggtccgcttc ttcgcctgtg agcaggacct ctcacacgat ccgatcgacg
      421 acccgctgcc ggaggcggtg gctaccggca aggagccgtt ctggatcgtc ggtgccatgt
      481 ccggcgggta gcgattcagg ttcctggagg cagcgtgcca ccagcccagg ccggtgggga
      541 ttcggcgcgg ttggcccgac cacacctctt gccagaattg gggatagtcg gcacaggggt
      601 ttgcctgcaa acgctcaccc ccggccggtg caggaccggt cgggggtgag ctaggcatct
      661 cttgagtgcg aaatgccgtg caggccgggg acggaccgtc gggatggccg acccgagggc
      721 agtatcacca aactgtgata tcggcagcct agcgcggcag gccgaacccg atcagcatgc
      781 gatcgtgatc acgcatgtca cggcgcgcct cgtcggcggt cgggtacccg gagccggcgt
      841 ggacgacctg gccgtagtag cgatcgaaac ggcgggcccc attggcattc ttacgagtga
      901 acagcttgcg aagtgttttc atcagtgcga tctcctggcg ttgcattacg tgcggatgga
      961 aaaccccgat gtctcccacc gtcggtatga taatatcaca ctatctcgat ttaagcatga
     1021 aacgtgaaca aatgctcaaa ttcggacgca agcatggcgg gttggcacac acggactgcg
     1081 gaaattcggg cgaaatcggg agcagcgctg tcagcccagc ccgagcgcta cccgtccctc
     1141 ggcaacggtg gtgagggagc cggtcacgaa gatcaccgat tcggcatatt cccgcgccgc
     1201 ccctataccg ctggcgacat cggccgctac ctcgcatcgg ccgccgaggg cctcgatcgc
     1261 cgcagcgaca gtctcggcgg gcagggcgcg cggggagttc ggcgcggcgg cgatccagcg
     1321 gtcggccaca tcgaggaggg gggtgatcac cggcccggga tccttgtcgc gcagcatacc
     1381 gaccaccaca gtcaccgact ggttggggaa gcgatcccgc agcgcagccg cgagggctcg
     1441 agtggcggct ggcgtgtgtg cgccgtcgag cacggccgcc cgtccatccg gcagcggcac
     1501 gacctcgaaa cggccgggca ggtgggcgcc cagcagcccg gcacggacga ccgcctcggt
     1561 ggtcgtcggg tcgaccaggt aggtggcagc gatggcgagt ccggaattgt cgacctggtg
     1621 ctggcccggc aacgcagtcg ccagctcccg gaaggtgccc cacggcccct cggcgccaaa
     1681 tgcgcggtcg gtgccggtgg ttcgccagtc acgccccgca accagcagcg gcgagccgag
     1741 ttcggtggcc cggcgctcga tcacgtgcat gccatctgac ggttgggcgg cggagaccac
     1801 gggtcgcccc ggcttgatga tgccggcctt gttggcggcg atctccgcca tggtgtcgcc
     1861 gaggatggcg gtgtgctcgt agtcgagcgg ggtgatgacc gagacagcgg ggtcgacgac
     1921 attggttgcg tccagcgtgc cgccgaggcc gacctcgacg accgcgactt cgcacccttc
     1981 ctgccggaaa tggagcagtg ccatcgcagt ggccagttcg aacgcggtta cctcgcccag
     2041 ctcgggtgag gccgtctcaa tggcctcagc ggcggcgatg ctgtcggcgg tgagcgtggt
     2101 aaacgcttcg ggcgtgatgg atcgatcgtc gacaacgaaa cgttcgtgcc acgagtgaag
     2161 atgcggtgag agatagcggc cggtccgtcg tccggtggcg cgcaggatag cgtcgatgaa
     2221 gacgcaggtc gagcccttgc ccttgctccc ggcgacatgg acgatcttgt acgaccgctc
     2281 ggggttgccg agacggtcga gcatcgcctg cgtacgtttc agcccgaggc gcgcggcgtc
     2341 gtcaccagcg aaggggttgc tgatgaaacc gcggtcgtat ccggagcgct cgtggatcgc
     2401 cgccagcgcc tgctcgtgcg tctcgatcgt gaccaccgtg ccgttccctc cgtagacagg
     2461 agaggggccg gcccggtggt cagcccctct cacggatgct gtgttgtcga tgatacgcgc
     2521 tgggctattc gacgatcagc gcagtgacca tgccgaacat gccgtgcttg ctctcggcat
     2581 ggctgaggat gtggcagtgg aaggcccacg tgcccggctc ggtggcttcg acaatggcat
     2641 cccagcgctc accgggggcg atgttgagcg tgtcgcagac gaacggctgc ggcagcgcgt
     2701 atccatcctt gtgcgtcacc gtcatcggca tgccgtgcag gtgcatcgga tggatcatca
     2761 gcccctcgtt catataccgg acccggatct tctcgccctg cttggcggtc agcggctcgg
     2821 ttgccgggaa gctcttgcca ttgatcgaga atccgccgat ggggccgtcg ttcaggatca
     2881 gcgtgtactc cttgtcgtac tccggctcgt tcgacggatc cttcggctca acgatgaacg
     2941 cgccgagcag gccctgcgtc acctggaacg ccgagttgtg gtgcgagtgg tacatgtgcg
     3001 agccactgtt gccctcgcga atggtgaact cgtaggtgaa ggtctcgccc ggcttgaccg
     3061 gtggctgggt gatgaacgga acgccgtcca tcttcacggg gacgaccagt ccatgccagt
     3121 ggattgccgt gctctcgggc agctcgttgg tgagatgcac gcgcaccttg tcgccctcgg
     3181 tgacgcgaat ctcggggccc ggcacggcgc cgttgtagcc ccacgcctcg gcgtactcgc
     3241 ccgggcggaa ctcccagtcg aagacgctgg cggtcagctc gaacaccttg acgtcgccat
     3301 ccatggtgta ctcgagcggc tggttgccga gaccttcggt cggtgccggg aaggactgga
     3361 tgccctcccg gtgcatctcg tccatctcgt cgacggtcag ctcgccgtcg tcgtcgccat
     3421 gcccctcgga agccatagtt cgctgtccgg caagcgctac cggtgcggcg agagcagtcg
     3481 cgccgaggct gccaacgaag ttacgacggg tgaacggagt cttgcgtgcc ttcatggatc
     3541 ttccttcttt aggtgatcat gcggttcgtc ctctcatgca gcgaatgata tcaccgccag
     3601 aggttcgaga aaatcgtagc atgcgggcaa atgaattccc atatcaggaa cgtgaagaat
     3661 gggctgaagt cgttacagaa tcgttacagt ttgggaatgg gtgcgctacc tgctcataga
     3721 tgggccgcat ttggggccga tgggcaggaa ttgcctgtgg agggtgatac ctggcaaggc
     3781 aaccgcgcct tggctgagaa cgtagaatcc ttgtggggtg catgggatac cgtatcggta
     3841 tcgcagcctt gccccgacgg ttcgcgcaca gaggcaccat ggcacgaaga tctacactgc
     3901 cgcagagatc gcggtccgca aatctgctga gcacggtcct tttcatcgcg gccatcggct
     3961 tcgccgccgc cgcaatctgg gtgtggtaca ccgatgactc cgagtcgagg cccggcgagc
     4021 caccgccggc ggagcgcatt gaagacatcg acctcgcgca tgtgctgagc gtgctcaagg
     4081 agcgcaacga cgactggacc tacagccgga acccggccac ggcgagcaca gatcagttca
     4141 atgccccggg gcaggcgctg cagctcgatg acgcgctgct cttcgtcttc atcttcactg
     4201 gcgctggcgc ggaggagcga atcgccgccc gcgaggccgc gagcgagcgt gtcgatctcg
     4261 aaaccatgac gctcacgaca ccctcgggca cagtgatcaa tgacaatggt gaaccactct
     4321 ggatggcgga gcacagcaac gtcatgacga tcctcgtcgg tggcgatcag gcactggcca
     4381 acgaggtcgc cgaagcgctc accgatctcc cgtaaccttc attgcaggac gtcccatccc
     4441 atgaccgatt cgacccgccc cgatgccaat gccatccgcc cgcccgcacc cagtgccggc
     4501 gagccggtgc cgaccctcgc tgacgacgac accgatcctg accgggagca gcagggacgc
     4561 atcggctatg gtcgctgggg gcgcctctcg ccgatcgggc tggcactgct gatccttgtg
     4621 acgctcggcg tgatctgggt aatggaccag gacaacgccg acgacgagac tgacgggctc
     4681 ccgagctcaa cgacgaccgg cgagctgcag gacccgcggc cggcgccaga gttcgaactc
     4741 gcgctgatga acggcgaggg caccgtctcg ctggaggaac tgcgcggcaa cacagtggtc
     4801 gtcaacttct gggcatcatg gtgcgccccc tgccgtgagg agatgccggc gctgcaggag
     4861 agcgccgagg cgcacgccgg tgacgaggtg gtctttgtcg gcgtcggcgc gaagaacgac
     4921 aagcaggacg cggccgaggc gttcgtcgag gagttcggca tcacctaccc catcggccgc
     4981 gacacggagg gcggcgactc cctgagcggc cagatcgaaa aggcgttcgg ggtgcccggc
     5041 tacccggcga cgttctttat cacccccgag ggaatgatca accagatcgt cttcggcccc
     5101 ctcgagatcg aggacttgga cacctacatc accaacacca ccgactgaca ccaccccacg
     5161 gaatcccacg caagctcgta gactcggccc tcggtgtccg gtttcctcga tgatgcctgt
     5221 gtaaggtgaa ccagtcagcg cgatgatcac ctgtagtcct gcgccgccag tgcgcgagtt
     5281 cgttgtggca ggcaacattc gattcctgtg accaacccag ccgtcatgct gagccgcgaa
     5341 tggccgatgg gggattgaat cgacggttaa ccggcccgat cgaacagcgg cgcccgtgac
     5401 gacgcccgcc catctgcagg cgggtctgtc agaatctccc cctgttctcg gggaacctgt
     5461 gtcggtgatg ctgggcccca ctctgggcgc agccacgcgg gtctaccgga gcaatcgtgc
     5521 cttacgcttt caccacctcg tggagagcga tgtcttctcg acgctgcata ccat
//
LOCUS       DASYWL010000034         4811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_526768, whole genome shotgun sequence.
ACCESSION   DASYWL010000034 DASYWL010000000
VERSION     DASYWL010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4811
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526768"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VGR22_01275"
     CDS             complement(<1..746)
                     /locus_tag="VGR22_01275"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2127231.1"
                     /translation="MGGQDYNELAGAYRRHREPHPDVIDALIPHGEVTETSHVLEVGC
                     GTGNYIHEIARRTGATCTGVDPSREMLRHAESRAELPMNRGAGHPEVTFVLGSAEDLL
                     LADRQFDLVYSVDVIHHIDDRDAAARETRRVLKPGGIAMVVTESEEDLRLRTPHVTYF
                     PDIVEVELRRYPSIETIQRELAAAGLEIGETIGVSRPVEVTETAPFRDKAYSSLHLID
                     EESFRDGLARMEADVSVGRFQVSVDTPSLRP"
     gene            complement(777..1598)
                     /locus_tag="VGR22_01280"
     CDS             complement(777..1598)
                     /locus_tag="VGR22_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2127232.1"
                     /translation="MSDNVIWPREGDLEQGWFAVDSPAEGVWRIQEPLHDENVKSFLV
                     VGSERAALIDTGMGIGNIRDVVERITSLPVVVINSHAHWDHIGDNARFGEIWIHEAEA
                     GDLLTGVSNDILRDWFGPDRLLGPLPPHVDVSTVTYPPTPPTGTLQGGEEIDLGDRSL
                     EVIHSPGHSPGGIALWDERNRILFTTDVAYPCMLLVHDRKDLPVYLQSLERLLALDPL
                     PELVLGSHCDVEMPVIMLAAQRDAIAGIIDGQEPTREVEEGRLLWELEGFSVKLG"
     gene            complement(1742..2956)
                     /locus_tag="VGR22_01285"
     CDS             complement(1742..2956)
                     /locus_tag="VGR22_01285"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV2127233.1"
                     /translation="MGQTRTPELSDVTRHPGRDATAAWWRGAAIVGIALLVALTAADL
                     FWLLVEPLTLVLVAIVIAQALSPLVAFVEERGLGHGVSVIAVYLVFVSLFGLLGWILV
                     PMLITEGQALVSDLPAMVSQARSWVSSVIPRDGSSSNQLISWAETGVQRFSNQLLSLP
                     ITIFTSLLDFVVIVFMSAYWLIFTPSLRRFTLSLFPEEKRLWVDSVLDEMGATMGGFV
                     RGTIIDGAIIAVAVYIGLTIIGLEYVLLLAVISGIGELVPIIGPIVAAVPAVVVGFLD
                     SPQKAGIVLLFFLVLQQLESNLLLPLIMHNQADVPPLMSLVAVVIGGALGGILGAILA
                     IPIFGALRILIVRVVAPAERDWTGVDDAPHEVGTEEETAPDEIVEEVDEEKENGHQDH
                     SEARRTSRSKSR"
     gene            complement(4130..>4811)
                     /locus_tag="VGR22_01290"
     CDS             complement(4130..>4811)
                     /locus_tag="VGR22_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2127234.1"
                     /translation="LDSALMVARDIGDRTQIASALTGLAHIVHLEGEHRAAVDLMRDA
                     MRSFERGRHLAAALMCLDGIANMALALGDGRHAAWCIGAVDGMTERTGFARQEAVQGE
                     HQARVDAVIAAIGRDAWHEAWAAGRALEPEAVLADAYAWQPPIGVVSSGTTTAVSRMP
                     EEAELLSPRELEVLRLMADGLTNQEIAEALYLSHRTATSHASNILGKLGLTTRTAAVA
                     YAIRSGLA"
BASE COUNT          862 a         1630 c         1473 g          846 t
ORIGIN      
        1 ggccgtaacg atggtgtatc gacgctcacc tggaatcggc ccacgctgac gtcggcttcc
       61 atccgcgcga gaccatccct gaacgattcc tcatcaatca agtgcagtga ggagtaggcc
      121 ttgtcgcgga atggcgcggt ctccgtcacc tcgacgggcc gtgagacgcc gatcgtctcg
      181 ccaatctcga gtcctgctgc cgccaactct cgctgaatcg tttcgatcga cgggtagcgg
      241 cgcagctcga cctcgacgat atccgggaag taggtcacat gcggcgtgcg gagacgaaga
      301 tcctcctcgc tctccgtcac caccattgcg attccgcccg gtttcagcac gcgccgcgtc
      361 tcccgcgctg cggcgtcacg gtcatcgatg tggtggatca cgtcgaccga atagaccagg
      421 tcgaactgcc gatctgccag cagcaaatcc tctgccgatc ccaagacgaa ggtcacctcc
      481 gggtgcccgg cgccgcgatt catgggcagt tccgcccgtg actcggcatg gcggagcatc
      541 tcgcggcttg ggtcgacacc ggtgcatgtc gcgccagtcc ggcgggcaat ctcgtggatg
      601 taattgccgg tgccgcatcc aacctccagc acgtggctcg tctccgtcac ctcaccatgc
      661 gggatgagcg cgtcgatcac gtccgggtgc ggctcccggt ggcgtcgata ggcgcccgcc
      721 agttcgttgt agtcctgtcc gcccatcccc ctgtcccttc ttccctgcgt cgcctcctag
      781 ccgagcttga cgctgaagcc ctcgagctcc cagagcaggc ggccctcctc gacctcgcgc
      841 gtcggctcct ggccgtcgat gatccctgcg attgcatccc gctgtgctgc cagcatgatc
      901 accggcatct cgacgtcgca gtgcgagccg agcaccagtt cgggtaaggg atcgagcgcg
      961 agcaaccgct ccaacgattg caggtagacc ggcaggtcct tgcggtcgtg cacgagcagc
     1021 atgcatgggt aggcgacatc ggtggtgaag aggatacggt tgcgctcgtc ccagagcgca
     1081 atgccacccg gcgagtggcc ggggctgtgg atcacctcga gactgcggtc gccgaggtcg
     1141 atctcctccc caccctgaag cgtgccggtc ggtggcgtcg gcgggtaggt gacggtactg
     1201 acatcgacgt gcggaggcag cgggccaagc agccgatccg gaccaaacca gtcacgaagg
     1261 atgtcgttgg agacgccggt caggagatcg cccgcctcgg cctcgtgaat ccagatctcg
     1321 ccgaaccgcg cgttgtcacc aatgtggtcc caatgggcat ggctgttgat caccacgaca
     1381 gggagtgacg tgatccgctc caccacgtcg cggatgttgc caatgcccat gccggtgtcg
     1441 atgagagcgg cgcgctcgct gccgaccacg aggaatgatt tcacgttttc gtcgtgcagg
     1501 ggctcctgaa tccgccagac accctcggca ggcgagtcca ccgcgaacca cccctgctcc
     1561 agatcaccct cgcgcggcca gatcacgtta tcgctcaccg ctcacccctc cccgggacgg
     1621 tcacacttcg cacggcctcc gcccaccggg cgaatgatgc caccccgatc caacctcacg
     1681 catgccggac catgcgcacc tccacgatcg tctgcccgag gccccagcag aggacactcg
     1741 gtcagcggct cttgcttcgg ctcgtgcggc gggcttccga gtggtcctgg tgcccgttct
     1801 ccttttcctc gtcgacctcc tcgacgattt cgtccggggc tgtctcttct tccgtgccca
     1861 cctcgtgggg cgcgtcgtcc acccccgtcc aatcgcgttc ggcgggggcg acgacgcgca
     1921 cgatcaggat acgcagcgcg ccgaagatcg ggattgccag gatggctccg agaattccac
     1981 cgagagctcc accgatgacg acggcgacca gcgacatcag cgggggcaca tccgcctggt
     2041 tgtgcatgat cagggggagg agcaggttgc tctccagctg ctgcagaacg aggaagaaga
     2101 ggaggacgat gcctgctttt tgcggagagt cgagaaaccc caccacaacc gccggcactg
     2161 cggccacgat cggcccgatg atcggaacga gctcgccgat accagagatg acagccagca
     2221 gcaggacgta ctctagccca atgatcgtaa gaccgatgta caccgcaact gcgatgatcg
     2281 ccccgtcgat gatggtgcct cgaacgaaac cacccatcgt ggcgcccatc tcgtccagca
     2341 cactgtcaac ccacagccgc ttctcctcgg gaaacaggga cagggtgaat cggcgcaggg
     2401 aaggggtgaa gatcaaccag taggcggaca tgaacacgat cacgacgaag tcgagcagcg
     2461 aggtaaagat cgttatcggc aaggacagca gctggttgct gaatctctgt acaccagtct
     2521 ccgcccaact gatgagttgg ttggacgaac tgccatccct tgggataacg ctactgaccc
     2581 agctcctggc ttgagaaacc atggctggca aatcgctcac gagggcctgg ccctcggtga
     2641 tgagcatcgg caccagaatc catccgagca ggccaaagag cgagacaaac accaggtaga
     2701 cggcgatcac cgaaaccccg tgcccgagtc cccgctcctc cacgaacgcg acaaggggcg
     2761 acagcgcctg cgcgatgacg atggccacca gcacgagcgt caaaggctcc accaggagcc
     2821 agaagaggtc ggcagccgtc agcgccacca gaagggcgat gccgacgatg gctgcgccac
     2881 gccaccaagc cgccgtggcg tcccgtcccg ggtgcctagt gacatcgctc agctccggtg
     2941 ttcgcgtttg tcccattcgg tactcctcgt ctgttgcgct ctactacgtc ggcacggagt
     3001 cccgccgctg ctgcgcgact ccgtcgttct accgatcgtc tcattgcatg attccggcca
     3061 ctggtacacg acctccggcc tttgtccccg gcacaaggga tccacctgcg agatcgaggt
     3121 caaaggctgc atgatgcctg gaatgacgac gacccggccg gtggctgata ccggctcggg
     3181 tcatcaaggg ggaggtgggg ggacaagccc ccgcgggggc cacggcatca ccgtggatcg
     3241 aggccgacca ttgccacagc acccggcggg gcgctgactg tcgcatccgc cgtcccggac
     3301 cgcaaaatgt ccggagggca acggcccgag aggttttgcc gattcggccg ctggtcgcaa
     3361 gggcgacgcc gccgatcatc gacgcttgga aaactgatcg tgcgcactcg tcgagccaac
     3421 cgctgaagac tgactatggt gccggctgcg acacgtgtcc gtttcacctg tctccatagt
     3481 gcgctctccc gagtctgcgc gcatccgaaa aagtacgtat ttctctgaca gcgtcgcatg
     3541 caccaattac ggacgtcagt cacctccgcg acgcagtgac accgtgccgg gcgatctatc
     3601 ctccggcacg gtgcctcacg cgagaatggg aatggtgatc agcaaaggcg tggttgtccc
     3661 agtaatctcc ggctacttca atctgactgc tggcgcccac caccgccgcc actgctgctg
     3721 gcgttgggga tgagctggtc ggcgctgacg ctgctgactg cgagctgctg gcaaggaccc
     3781 catggcgaca gtgacgaggc gggcgcaagc gagcgaagac tgatagcgga gcgattggcc
     3841 atgccggacg cctatctgag tggtacgagc gggtcgcacg gatcggtcgt cggccgtaac
     3901 tccagcatgc gctcattgtc ccgccacaca tcgcacgatc tggggtgtcg cctcctctcc
     3961 ttcctgcgct cccgctttcg tcgtttgtat agtcggggtc gccctggtgc tgtgatctct
     4021 cctccagatc accggggatc ccgaggcacg gcaggtagaa ccgtgccggc tatcgccggc
     4081 gccccatcgt tgccccgact ccgtcccgtg agtgatcacg gtcggacgtt caggccaggc
     4141 cgctgcggat ggcataggcc accgctgcgg tgcgcgtggt gagcccaagc ttgccgagga
     4201 tgttgctggc gtggctggtg gccgtgcggt ggctgaggta aagcgcctcg gcgatctcct
     4261 ggttggtcag accgtccgcc atcaatcgga gcacctccaa ttcccgtggc gagaggagtt
     4321 cggcctcctc cggcatgcgc gacacagcgg tcgtggtgcc agaactcacg acgccaattg
     4381 ggggctgcca ggcgtatgcg tccgccagca ccgcctcggg ctccagcgcc ctgccggccg
     4441 cccaggcttc gtgccaggcg tccctaccga tcgccgcgat caccgcgtcg acccgtgcct
     4501 ggtgctcgcc ctggaccgcc tcctggcgag caaacccggt tcgctccgtc atgccatcga
     4561 ccgcgccgat acaccacgcc gcatgcctgc catcccccag cgccagcgcc atgttggcaa
     4621 tgccgtcgag acacatcaac gctgcggcga gatgcctccc tcgctcgaaa ctgcgcatgg
     4681 cgtcccgcat caggtcgaca gcggcacggt gctcgccttc gagatgaacg atatgcgcca
     4741 aacccgtgag cgcgctggcg atctgcgttc ggtcgccgat atctcgcgcg accatcaggg
     4801 cgctgtcgag c
//
LOCUS       DASYWL010000035         5644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_226519, whole genome shotgun sequence.
ACCESSION   DASYWL010000035 DASYWL010000000
VERSION     DASYWL010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226519"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..552)
                     /gene="rsfS"
                     /locus_tag="VGR22_01295"
     CDS             complement(169..552)
                     /gene="rsfS"
                     /locus_tag="VGR22_01295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEV2127235.1"
                     /translation="MTQDQQTLSLTTEEEIRDFAITIADILADTPASHTRVLDIRGMS
                     SLADFFIITTGENERQLRAISRHLSDEVSKRKIRPARVEGEPASGWILVDYLDVVVHI
                     FEADMRQFYDLEELWQDATVLLEIQ"
     gene            complement(601..672)
                     /locus_tag="VGR22_01300"
     tRNA            complement(601..672)
                     /locus_tag="VGR22_01300"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(638..640),aa:Ile,seq:cat)
     gene            853..1041
                     /locus_tag="VGR22_01305"
     CDS             853..1041
                     /locus_tag="VGR22_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127236.1"
                     /translation="MAKTAGLQVDAERARRLRDELDSALDAVAALDDAIDASSRGTAG
                     ADTFDADWAGPDRENRRG"
     gene            1034..2458
                     /locus_tag="VGR22_01310"
     CDS             1034..2458
                     /locus_tag="VGR22_01310"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEV2127237.1"
                     /translation="MGSLSLDTITLTTAVAAMRARKLSAVELLAAVHQRYVETEPVLH
                     GWVERFTEARAQAVTADRRIAGGATAPLLGVPIGIKDIFDVAGSPTRCNSPSREDVPV
                     AHRDAEPVHRLRKAGTVLLGKTVTQEFAAGVVSAPARNPWNPHRVPGGSSGGSAASVS
                     AGTSLGALGSDTGGSIRIPASVTGTVGLKPTYGSLDLTGTFPLSVSLDTVGPITRTVA
                     DAVAMHLVLANRHAEVLEAVAHLDRLAVEPSLTGVRLGVMRGFFNHRLQSGVAEAFEQ
                     AVRALAEQGAELVEIDWQLAEAARASALLISRVESAAVHHRLLRENPELIGDDVRYRF
                     EVGALLTGDTYLRAKEARRAARDSIRHIYREHELAAVVAPTTPAVAPSAARLEVTFSG
                     GAVENVGAALTRLTMPWNATGQPALSVPCGFDHDGMPVGLSFVGKPNDELRLAEIAQH
                     YEQATDWHRRRPSIGGRTDGLASP"
     gene            2455..3459
                     /locus_tag="VGR22_01315"
     CDS             2455..3459
                     /locus_tag="VGR22_01315"
                     /inference="COORDINATES: protein motif:HMM:NF036726.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HEV2127238.1"
                     /translation="MRTIVPEYLRAAVPDNGFHAWLENLASLDVSGLDICLPEGEELA
                     DELRWLDVPEHAIAEVLASRPDPTLHPDAWWLLERCVARLINHLGLIADMAPFPELPE
                     ELARQVPHLYLHVFVAMLPHTRAYHRERDIPGRLSQAILADLGRNVRVHERRYGEVGL
                     GVVNWITLHFRGLIYDLGRLQFERAQMRGTLANAMRERAVDARPDETVLSIHVPDSKG
                     PLTPEACDASIACAHQFFAEHFPEERYRFAVCHSWLLDPQLNRCLRPDSNIVRFQERF
                     ELADRLTEADRSIVQFVFGPVPDDPADLPQRTSLERAVREHLLAGEHWYVRNGWFRFD
                     "
     gene            3609..5021
                     /locus_tag="VGR22_01320"
     CDS             3609..5021
                     /locus_tag="VGR22_01320"
                     /inference="COORDINATES: protein motif:HMM:NF037429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vms1/Ankzf1 family peptidyl-tRNA hydrolase"
                     /protein_id="HEV2127239.1"
                     /translation="MGIDPTQSIQEQDYENALVVQARKIAERLAELQPSPKIPYVTVT
                     LDWRVEGTNPGRQPYYPEGTRPDDDKLRSQPDRPSEEREEDSARRRPARRVFEDQMDE
                     LVEAHGPHGDVYESLKADFERISNYLDHEIDPSAKGAFIVSCSGKGVFETVLFALPLT
                     TSVSTGPTPALTELVRLDEDFPTYGALLADQKNAHLSVISHGTTDHAVILEASGYPRK
                     QAQGGWSQRRYQARADERIEAFARDVAEETRKVMDDEGIGSLIIAGDEVITSVLDGEM
                     HESVKDRVIGTIHLDIDASESDLLESSMPVALKAERQRELEAVQHASDGIGADTFGAG
                     GSQKVVDALQAGQVMTLVMNDDFEGVGWADYTLPAYGVGDLPTNHPLGGEVSNLVEVD
                     LRDEFVRLAVQTGAEVEFIHTNIPDDATELDEIPEPGEGPPRSKAARMLDEHGGVAAI
                     LRYVVGDEPSEAESAAQAQA"
BASE COUNT         1019 a         1822 c         1865 g          938 t
ORIGIN      
        1 ccagaccacc gacaggagat gcacgagcga tcggccgagc gccgcaccga gaacgaaccc
       61 cactgtggcg gccccgatgc tggcgaacac gagcgtcagg acgctgttgt cggatttcat
      121 tgagcagtgt ctcctccagc gttgggtctt gcattgcatg acggcgcgct attggatctc
      181 gaggaggacg gtggcgtcct gccagagctc ctccaggtca tagaactgcc gcatgtccgc
      241 ttcgaagata tgcaccacca cgtccaagta gtcgacgaga atccagccgg aggctggctc
      301 cccctcgacg cgcgccgggc gaatcttgcg cttgctcacc tcgtcggaaa gatgccgcga
      361 gattgcgcgg agctgccgct cgttctctcc tgtggtgatg atgaagaagt cggccagcga
      421 cgacatgcct cggatgtcga ggacccgggt atgggatgca ggggtatcag cgaggatgtc
      481 tgcgatcgtg atcgcgaagt cccggatttc ctcttcggtc gtgagcgata gcgtctgctg
      541 atcctgagtc acgtgcacct ctcatacgtg cattgcgcac cgtctggaat cgccgcccgg
      601 tgggcccggc agggttcgaa cctgcgacca aggggttatg agccccgtgc tctaccgctg
      661 agctacaggc ccgttgcgcg tgcgatccta gtgtacccgc ggtcgcatct ctcgaccaac
      721 ggcccgggac aatagccgta gcgtggatgc ggggcaggaa cggctggacg gatgtgcggt
      781 gcgactgcta tcagcgaggg gagaggaatg caagggaatc gtacgcgtcc gacggcggag
      841 actgttctgg agatggcgaa gaccgcaggg ctgcaggtag acgcagagcg agccaggcgg
      901 cttcgcgacg agctcgattc cgctctcgac gcggtagccg cgctcgatga cgccatcgac
      961 gcaagctcgc ggggaaccgc gggcgctgac accttcgacg cggattgggc gggtcccgac
     1021 agggagaaca ggcgtgggta gtttgagcct ggacacgatc acactgacga ccgccgttgc
     1081 agcgatgcgc gcccggaaac tctcggcggt tgagctcctc gcagcggtcc atcagcgata
     1141 tgttgagacg gaaccggtgc tccacggctg ggttgagcgc ttcacggagg cgcgcgccca
     1201 ggccgttaca gcagaccgcc ggatcgccgg tggggcgacg gcgccgcttc tgggcgtacc
     1261 gatcggcatt aaggacatct ttgacgttgc cggaagcccc acgcgctgca actccccatc
     1321 gcgggaggac gtgccggtcg ctcacagaga cgctgagcca gtgcaccgcc tgcgaaaggc
     1381 cggcacggtg ctgctgggca agacggtaac gcaggaattc gcggcgggtg tcgtgagcgc
     1441 gccagcgcgg aacccctgga atccccaccg tgtgcccggt ggtagctccg gcggctcggc
     1501 ggcctcggtc agcgccggga cgtcgctcgg cgcgctgggg agcgacacgg gcggcagtat
     1561 ccgcattccg gcctccgtta ccgggacagt cggcctgaag ccgacctacg gaagcttaga
     1621 tctcaccggc accttcccgc tctccgtctc gctcgacacc gtcggaccga tcacgcggac
     1681 ggtagccgac gccgtggcga tgcatctcgt gctcgccaat cgacacgccg aggtactgga
     1741 agcggtcgcc cacctcgacc ggctggcggt ggagccctcg ctcaccggcg tgcgccttgg
     1801 cgttatgcgc ggctttttca atcatcgcct gcagtcggga gttgccgagg cgttcgagca
     1861 ggcggtacgc gcgctggcag agcagggcgc cgagctggtg gagatcgact ggcagctggc
     1921 ggaagcggca cgggcctcgg cacttctcat ctcccgagtg gagtcggcag cggtgcacca
     1981 tcgcttgctc cgggagaacc cggaactcat cggtgacgac gtgcgttacc ggttcgaggt
     2041 cggtgcgctg ctgaccggcg atacctatct gcgggcgaag gaggcgcgac gcgccgcgcg
     2101 ggacagtatc cgccacatct atcgagaaca cgagttggca gcggttgtgg cgccgacgac
     2161 accagcagtc gcaccgagcg ccgcgcggct ggaggtcacc ttttcgggtg gggcagtaga
     2221 aaatgttggc gcggccctaa cacgcctcac catgccgtgg aatgcgaccg gccaaccagc
     2281 gctcagcgtg ccatgcggct tcgaccacga cggcatgcct gtcggcctct cgttcgtggg
     2341 caagccgaac gacgagctgc ggctcgcgga gatcgcccag cactacgagc aggcgacgga
     2401 ttggcaccgg cgacgaccgt ccatcggtgg gagaaccgac gggttggcgt cgccgtgaga
     2461 accatcgttc ccgagtatct gcgcgctgcc gtccccgaca acggcttcca tgcctggctc
     2521 gaaaacctgg cgtcactcga cgtgtccggg ctcgacatct gccttccgga gggtgaggaa
     2581 cttgctgacg aactgcgctg gctcgacgtg cccgagcacg cgatagccga ggtcctagca
     2641 agccgaccgg acccgacact ccaccccgac gcatggtggc tgcttgaacg gtgcgtcgca
     2701 cgcctgatca accacctcgg cctcattgcc gacatggcac cgttccccga gctgcccgag
     2761 gagctagcac ggcaggtgcc tcatctttac ctccatgtct tcgtcgccat gctgccgcac
     2821 acccgcgcct accaccgcga gcgagacatc cctggccgcc tgtcccaagc gatcctggcc
     2881 gacctcggac gcaacgttcg cgtgcatgag aggcgatatg gagaggtggg actcggcgtc
     2941 gtcaactgga tcacgctcca ttttcgtggc ctgatctacg acctcggccg gctgcagttc
     3001 gagcgggcgc agatgcgcgg cacgttggcc aatgcgatgc gcgagcgagc cgttgacgct
     3061 cgaccggacg agacggtgtt gtcgatccac gtaccggact ccaagggacc gctcacgccc
     3121 gaggcgtgcg acgcgtcgat tgcctgcgcc catcagttct tcgcggagca cttcccggag
     3181 gagcgctacc gattcgccgt ctgccactct tggctgcttg acccgcagct caatcgctgt
     3241 ctgcggcccg actccaacat cgtccgtttt caggagcggt tcgagctcgc cgatcgcctg
     3301 accgaggccg atcgctcgat tgtgcagttc gtcttcggcc cggtacctga tgatcccgcc
     3361 gatctcccgc agcgcacctc cctggaacga gcggtgcggg agcaccttct tgccggagaa
     3421 cactggtacg tccggaacgg ttggttcagg ttcgactagc gcctgtcggg ccaaccagcg
     3481 gccggtcgcg actgaccgcg ggcgcccgct ggcatggatt ctgcaccgac gcttcagggc
     3541 acgtatgccc gcacagcagt gatgaacaag aactgcccca cgacaggggg aaaggatcga
     3601 acgcgaccat ggggatcgac ccgacacaga gcattcagga gcaggattac gagaacgcgc
     3661 tcgtcgtcca ggcaaggaag attgcggagc gtctcgccga gttgcaaccg tctccgaaga
     3721 ttccctacgt cacggtgacg cttgactggc gcgtcgaggg caccaacccg ggacggcagc
     3781 cgtactaccc agagggcacg cggcccgacg acgacaagct gcgatcgcag cctgatcggc
     3841 cctccgagga gcgcgaggag gacagcgcgc gacgccggcc cgcgcggcgc gtgttcgagg
     3901 accagatgga tgagctcgtc gaggcgcatg gtccgcacgg ggacgtgtac gagagcctga
     3961 aggcagactt cgagcgcatc tccaactacc tcgaccacga gatcgacccg tccgcgaaag
     4021 gcgccttcat cgtctcgtgc tcgggcaagg gcgtcttcga gaccgtgctc ttcgcgctcc
     4081 cgctgacgac cagcgtctcc actgggccaa caccagctct gaccgaactg gtccggctcg
     4141 atgaggactt cccgacctac ggggcgctgc tcgctgacca gaagaacgcg catctctcag
     4201 ttatttcgca cggtaccacg gatcatgctg tgattctcga ggcgagcgga tacccgcgca
     4261 agcaggcaca gggcggctgg tcgcagcgca ggtatcaggc ccgagctgac gagcggatcg
     4321 aggcctttgc ccgtgacgtc gccgaggaga cccggaaggt gatggatgac gaggggatcg
     4381 gctcgctcat catcgcgggt gacgaggtga tcacttcggt gctcgatggc gagatgcacg
     4441 agagtgtcaa ggaccgcgtg atcggcacga tccacctcga cattgatgcg tcggaatccg
     4501 acctgctcga gagctcgatg ccggttgcgc tgaaggcgga gcgtcagcgg gaactcgagg
     4561 ccgttcagca tgcgagtgac ggcatcggag ccgacacctt cggcgccggc ggttcgcaga
     4621 aggtcgtcga cgcgctgcag gccgggcaag tcatgacgct cgtcatgaat gacgacttcg
     4681 aaggtgtggg ctgggccgat tacacgctgc cggcctacgg cgtgggcgac ctgcccacca
     4741 accacccgct tgggggtgag gtatccaacc tcgtcgaggt cgatctgcgc gacgagttcg
     4801 tgcgactcgc agtccagacg ggtgccgagg tcgaattcat ccacaccaac atccccgacg
     4861 atgcgaccga gctggacgag attcccgagc ccggtgaggg cccgccgcgc tcgaaggcgg
     4921 cacggatgct ggacgagcac ggcggagtcg cagcgattct ccgctacgtc gtcggcgacg
     4981 agccctcgga ggcggagtcg gccgcccagg cgcaggcgtg atcggcgctg tggcgacgcg
     5041 atgaaacgct ttcatgacgg gccgctcgtg ggagcggccc gtcggcatat ctgacccata
     5101 aagcgaaggt tgcccactgt cgacgcatcc ggtaacgcaa cgatcaagag ctccaaaacc
     5161 aagatcggca atcgagcact gcgcgatgcg cgggttcacc gactcgcagg caacaccttc
     5221 gccggagcta ttgggacgcc ggggttacga catccagggc tacgccaatc ttggcgcccc
     5281 aatcggacgc tggaccgctg gttctcgtgg cgtggcaggt atcatgccca ctcgcggtac
     5341 tacaccgtta gacaggccac cgaggcgctc gcttccagtt ctgaccgcgc tcgagatccg
     5401 gcgacacggc tcgttgtcgg caccaagatg ggtcaccact tgctgatcac tggagcggac
     5461 ctccaggctc tgcgccagta tgccgggcgc cttccagtca cgtcaccgtc acccggtatc
     5521 gtcgaaacgc aacgatgaga cgcgagcctc gcgatggcaa cccgacggat cgcatcggtc
     5581 atcgtctgca gcggcgcatg ctcaacgatg actgttgcgc ctgaatcgcg acacaagtcg
     5641 gtgc
//
LOCUS       DASYWL010000036         1559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_189166, whole genome shotgun sequence.
ACCESSION   DASYWL010000036 DASYWL010000000
VERSION     DASYWL010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189166"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1359)
                     /locus_tag="VGR22_01325"
     CDS             complement(154..1359)
                     /locus_tag="VGR22_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEV2127240.1"
                     /translation="MTETNMPLIELLQKQDDGDFLRAVAEAVLQLLMEHDVEGLVGAG
                     RYERGDGRLTWRNGYRDRELKTRLGVLNLRVPKLRQGSYFPGFLEPRRTSEKALVAVI
                     QEAWIGGVSTRRVDELVQAMGLTGISKSTVSKLCKEIDERVAEFLQRPLSGEWPYLWL
                     DATYLKVRQGGRIISVAAIIAMAVNTDGRREIIGLGIGPSEAETFWTEFLRGLKGRGL
                     DGVKLVISDAHTGLKAAIARVFDATWQRCRVHWMRNALAHVPKGQHTVVAAAIRQAFT
                     QPDRDSAGQTWRHVADQVRGRWPKLAALMDESEADVLAYMSFPSQHRTKLHSTNPLER
                     LNKEIKRRADVVGIFPNEDAIIRLIGAVLFEQNDEWLGQHRYMQVEAFAQIDAAERDP
                     LLSITTQAA"
BASE COUNT          254 a          527 c          494 g          284 t
ORIGIN      
        1 gcgcagcact actgcgtcac ccgttaacca cctccgcgac accagcgaaa cggctggtac
       61 cgcagtacta atttttgcat cttcggctcg tacgacgacg gtcacgtccg tcaaggtggt
      121 ggggattcca ggatgaccta ctgagggcat cgctcaggcg gcctgcgttg taatgctgag
      181 aagggggtcg cgctcggcgg catcgatctg cgcgaaagct tcgacctgca tgtagcggtg
      241 ctgtccgagc cactcatcgt tctgttcgaa gaggacagcg ccgatgagcc ggatgatggc
      301 gtcctcgttc gggaagatgc cgacgacgtc ggcccggcgc ttgatctcct tgttgaggcg
      361 ctccagcgga ttcgtggagt gcagcttggt ccggtgctgg ctggggaagc tcatataggc
      421 gagcacgtcg gcctcgctct cgtccatgag cgccgcgagc tttggccagc ggccgcggac
      481 ctggtcggcg acatggcgcc aggtctggcc ggcgctgtcg cggtcgggtt gggtgaaggc
      541 ctggcggatc gcggcagcca ccacggtgtg ctgccccttc ggcacatggg cgagcgcgtt
      601 cctcatccag tgcacgcggc acctctgcca ggtggcgtcg aagacccgcg cgatcgccgc
      661 cttcagcccg gtatgggcgt cggagatgac cagctttacc ccgtccagcc cgcgtccctt
      721 gaggccgcgc aggaactcgg tccagaacgt ctccgcctct gagggaccga tgccgaggcc
      781 gatgatctcg cgccgcccgt cggtgttgac cgccatggcg attatcgccg ccacggagat
      841 gatcctcccg ccctgacgga ccttgaggta ggtggcgtcg agccagagat agggccactc
      901 gccggagagc gggcgctgca ggaactccgc gacccgctcg tcgatctcct tgcagagttt
      961 cgagaccgtg ctcttggaga tcccggtcag ccccatcgcc tgcaccagct cgtcgacccg
     1021 gcgcgtcgag acgccgccga tccaggcctc ctgtatgacc gccaccagcg ccttctccga
     1081 cgtccgacgc ggctcgagga agccggggaa ataggagccc tgccggagct tcggcacgcg
     1141 caggttcaat accccgagcc gggtcttcag ttcgcgatca cgatagccgt tgcgccaggt
     1201 caggcggccg tcaccccgct cgtagcggcc ggccccgacc agaccctcga catcgtgctc
     1261 catcagcagc tgcagcacgg cctcggcgac agcacggaga aagtccccgt catcctgctt
     1321 ctgcaggagc tcgatcagag gcatgttggt ctcggtcatc ggggtctcca tcggtccctg
     1381 gttgaagtcg ccaaactcca ccatagccga tgccctcgat ggccaccccg gcctacaccg
     1441 cggtcgtgcg ctgaatctcc accacgttct cggacgctaa ccagcttctg ctcttcgggc
     1501 gaaaccgggg gcgggggaga aattgatttt aacccctcgt ttcacgatca gcccgcacc
//
LOCUS       DASYWL010000037        14376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_658324, whole genome shotgun sequence.
ACCESSION   DASYWL010000037 DASYWL010000000
VERSION     DASYWL010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 14376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14376
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658324"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            367..2832
                     /locus_tag="VGR22_01330"
     CDS             367..2832
                     /locus_tag="VGR22_01330"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEV2127241.1"
                     /translation="MSADGATYTEPQIVGPADADAGPADRDGRTFGHLIMTDPAQYVR
                     YRTLDANGAPTVLPGLAFTYIDASHGPTGDDVHPEAFGGALTKTPIITRDRWGAAQAY
                     GGWDGGTDGWEADYRTVKHIIIHHSVTPQFRDPLVEIRSIHYYHAITRGWGDIGYNYL
                     VDFLGNVYEGRKGGENVVGGHAFQYAYGSAGICSMGNFTLESSTPESIAALVWITAWA
                     GRELDPLGKSDFHETPNCPTIAGHRDVYDSTCPGDGLYADLPYIRTAVAEVLAGSRNA
                     GIPSGYDPGDVIQTTVSGANLRSKPSTSGSIEARLERGKVLTIIEGPTTNDGYTWYRV
                     SGEQSAGWLATTTFSRSDAAPPQRDYEPNTRVEIAANLLNLRSEPDLWASVVARLPQG
                     DTATVVDGPALSGGLTWLKLDTNLGTGWVAQQFLAPSGRAWISSKFAVGDTVWVTTNP
                     LNIRTDTSLQSRIIARLWEGETGAVIGGPRRSAGIIWVQIRTDRGSGWVSEEFLEEGS
                     APPPQPVNGKFSVGDEVEVDTDKLNVRGGPGLDDRVVTVLWTGDEATITDGAVEADGY
                     LWYEIDAGHASGWVAETFLATAGSADPRASGFEVGTKVTVIEDSVFFRESADTTGRIF
                     WKLYRGDTGTVAGGTVQSDGYSWVLCDFSGTLGWVATPFLGRGTGARSSSTGLGVGIT
                     VNVTENKVNVRPEPGITDIVLGQLYEGATATIQEGPVEADGYTWYRITGARWEGWVTD
                     GYITTAVGGSIGLSSSVRVFDGELNMRSGPSTADDVVRVLPDGAIVEVLDGPERSDGH
                     EWYRVSSSRYGTGWVIAAWLERV"
     gene            2979..3566
                     /gene="rplI"
                     /locus_tag="VGR22_01335"
     CDS             2979..3566
                     /gene="rplI"
                     /locus_tag="VGR22_01335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEV2127242.1"
                     /translation="MKIVLRQDVPKLGEAGSIQTVADGYARNYLIPKGMAVVATEGEM
                     KTAQHNMKVRERKVSRQEEQLQSLADKIQGTRLEFTANAGEQGRLFGSITASDIMVQL
                     NEKIGEKIDRRKLQLTDPIRTVGEHELEIHLVGKLRPTITVLVQPEGGLQPETDEMAA
                     EADVPEELKASEEDLAMDEEAAAEVTDEEDESANA"
     gene            3657..5003
                     /gene="dnaB"
                     /locus_tag="VGR22_01340"
     CDS             3657..5003
                     /gene="dnaB"
                     /locus_tag="VGR22_01340"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEV2127243.1"
                     /translation="MDTQVDRLPPHNIEAEQSVLGSLLIDRDAIIRVASYLKVEDFYV
                     SANATIYSAIRDLYNRREPTDFVTLSDELERREMLDDIGGVGYLSSLLSAVPTAVHVE
                     YYGRIVERTATLRRLIDAGTSIASIGYQDNIDTEDALDAAERALFNVTQQRSTKDFQS
                     IDEVIDRFFDQLEFLQTNRGELVGVPTAFTDLDRLTGGLQRSDLVILAARPSMGKTAF
                     SLGMAYGAAVKHGRTVGIFSLEMSAEQLVQRLISTETGIDSHRIRLGNISDREWEEIS
                     RAFGRLSEARIFIDDSANAGIMDVRSKARRLQAEQGLDLVIVDYLQLMSGRRTENRVQ
                     EISEISRGLKGLARELNVPVVALSQLSRAVESRSDHRPMLSDLRESGSIEQDADIVMF
                     IYREEKYDEHSDKKGIAEIVVAKHRNGPVASVNLRFFESTARFADLEMYRADFDEE"
     gene            5006..5689
                     /locus_tag="VGR22_01345"
     CDS             5006..5689
                     /locus_tag="VGR22_01345"
                     /inference="COORDINATES: protein motif:HMM:NF018914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEV2127244.1"
                     /translation="MPTRRRQDNTPFAGFVDIDREAVSIPQRFFTEVLPEITSIGELR
                     VTLTFFRMLTDHGGVEAPVPEQTLLADRLLQQAFRVDGIPTGDVRDAALNALDRAVAR
                     GTLLRVVSRSGRASTMWYFLHTTVTRELVAAIQRGAVAPPQVMWSDNHTPEINTDPPT
                     PFYLYEQNIGPLTPMVADRISRAMDDYPPVWIEDAIAEAVTYNRRSWRYIERILESWS
                     EQGRPDRSS"
     gene            5721..6539
                     /locus_tag="VGR22_01350"
     CDS             5721..6539
                     /locus_tag="VGR22_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2127245.1"
                     /translation="MKRIGDVMREIPIPKQPTTGQASGAVRIRKDAVRCPICNDAGYL
                     RANVPVGHPSFGRLFPCECKQRELDERRDDELRRLSNLDAFSDYTFESFDPDIPGMEE
                     AYDVAIAFAQEPRHNWLFLSGPCGVGKTHLAVAIARYAMEWHRMSVYFAVVPDLLDHL
                     RSTFDPKSGSAYDDRFATIKNAPLLVLDDLGTENATPWAKEKLYQIINHRYSEQMPTV
                     ITTNVDLSSSGKIDERIYSRIMDHRLTQHIAIDAEDFRLAGDPRRVRRTTGRHR"
     gene            6892..7788
                     /gene="cdaA"
                     /locus_tag="VGR22_01355"
     CDS             6892..7788
                     /gene="cdaA"
                     /locus_tag="VGR22_01355"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HEV2127246.1"
                     /translation="MSQIGWLLSQLLNPRAVLDILVVALIIYWLLWVAQGTRALHVIR
                     GIVTLFALLVFLGWALDLSTLNWILNNSWPGLVIAIPVIFAAELRRGFEQLGHTGTWL
                     RLPFTANTEDTGLDEAVDEIVRASAQLSRLRYGALTVIERETGLQDFASRGVQIDGIL
                     TRQLLINIFFPNTPLHDAAVLVRNNRILAASVVLPLTDNISATSNLGTRHRAAIGVTE
                     ESDAIAVVVSEETGQMSVAHSGRLIRNLDQDRLRRVLRTLLSLDQPRRREAGYPFFSF
                     ANRNGEDRSHSSRRTERRQTGD"
     gene            7814..9124
                     /locus_tag="VGR22_01360"
     CDS             7814..9124
                     /locus_tag="VGR22_01360"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HEV2127247.1"
                     /translation="MLQNVRNFLQEHLTGQQMARFALSLLVALVLWGWVTQLQNPVEA
                     QRYAELEIVPPELPGPLEIVTNLPRVTVSITGVSSQLEEISRADITVTVDPSRVDGSG
                     MYQLPVVVELDGEVREMRVSPDTVSVEVEELVSRNFPLTVENQAPDEESGRIVDTDPE
                     VSEVTITGPAPAVDRIDRIVLPVSAQGHARDFVEVIEPFAVDENGQRVQEVIITPGNV
                     RTVVELEARGKAVSVVPQVVGSPAERYVVQQQIAVPSMVIVDGPPEVLDSLLFVNTEP
                     VDISGATDSLSEVVPLENLPEGATLVEPEENRIEVRVSIGPSGGTANRISNMPVAVED
                     IRDGLDATVDPGAVAIEVQAPSDVLTSLTPDDVSVWIDASGLEPGIYALQPQVEVPDD
                     VEVIQTEPEEVVVVIITNGSATPVAPAGLRGHRALTVNEYMVDS"
     gene            complement(9199..9843)
                     /locus_tag="VGR22_01365"
     CDS             complement(9199..9843)
                     /locus_tag="VGR22_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2127248.1"
                     /translation="MNRSSRSAEKWRLIIADDESLIRLDLREMLTHLGYDVIAEASDG
                     RSAVDLARKLRPDLLIMDVKMPEVDGIAAAEELTRERIAPVVLLTAYSDQGLVERARM
                     AGVVGYVVKPFREAELMPVIELARARFEEFRTLEREVGSLKDALETRKVIERAKGVLM
                     ETHGMRESEAFHRIRKTSMDARKSMKEVAEAILLAHDLEYSVSTGSDDDESGMS"
     gene            complement(9953..10026)
                     /locus_tag="VGR22_01370"
     tRNA            complement(9953..10026)
                     /locus_tag="VGR22_01370"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9990..9992),aa:Pro,seq:ggg)
     gene            complement(10138..10527)
                     /gene="rplL"
                     /locus_tag="VGR22_01375"
     CDS             complement(10138..10527)
                     /gene="rplL"
                     /locus_tag="VGR22_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEV2127249.1"
                     /translation="MTMAHEALIEQLENMTVIQLNELVKELEEKWGVSAAAPIAAGAV
                     AGAPGAAGGAAAEEEQTEFDVVLTDVGGNKIQVIKAVREITSLGLKEAKELVDSAPKA
                     VKEGVAREEADAAKAKLEDAGAKVDVK"
     gene            complement(10597..11145)
                     /gene="rplJ"
                     /locus_tag="VGR22_01380"
     CDS             complement(10597..11145)
                     /gene="rplJ"
                     /locus_tag="VGR22_01380"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEV2127250.1"
                     /translation="MPTAQKAKQIDEIAESLERSQLTVVASYRGLTVTQLQDLRRGLR
                     DSNAEFTVAKNTLTRIAAERVGVDIPGEHLEGPTALMFAYEDVVAPAKALNDYARASR
                     GVLELKVGMMEGQTLTTSDIDVLASMPPREELLGKLVGMLASPMARAVGVLNGPSRSI
                     AYLFNARAESVGGEGGEAQAAD"
     misc_feature    complement(11198..11328)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(11341..12063)
                     /gene="rplA"
                     /locus_tag="VGR22_01385"
     CDS             complement(11341..12063)
                     /gene="rplA"
                     /locus_tag="VGR22_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEV2127251.1"
                     /translation="MPKHGKRYQQAAELVEADRQYTLDEAIELLGKFPAPKFDETVEL
                     HARLGIDPRQADQAVRSSVTLPHGTGKNVRVLVFAAGEAERAALDAGADYAGTDEYVQ
                     QINNGWLAFDAAVAMADQMSKVGGLGRILGRRGLMPNPRSGTVVRELEDLPGVIRDLK
                     GGRVEFRNDRTGNVHTVLGKRSFTPEQLRENILAFVDALNREKPSGVKGIYCRKLSLT
                     TTMNPGMNLDVNDTINLASQLS"
     gene            complement(12131..12556)
                     /gene="rplK"
                     /locus_tag="VGR22_01390"
     CDS             complement(12131..12556)
                     /gene="rplK"
                     /locus_tag="VGR22_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEV2127252.1"
                     /translation="MAKKIRGYVKLQIPAGKATPAPPIGPALGQHGVAIMNFCKEYNE
                     RTQSMAGQIVPVVITVYEDRSFTFITKTPPAADLLRKAAGIEKGTDNPKTTKVGKVTQ
                     DQVREIAELKMQDLNAIDVDGAMKQVAGTARSMGIEVVG"
     gene            complement(12652..13251)
                     /gene="nusG"
                     /locus_tag="VGR22_01395"
     CDS             complement(12652..13251)
                     /gene="nusG"
                     /locus_tag="VGR22_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEV2127253.1"
                     /translation="MARQTLRERLAARRAEEAGVEAESEQGQWYVLHTYSGYENKVKK
                     TIESRVEAYDLEDRVFEIVVPTQEEIEIRNGQRHTVQKKVFPGYVLVRMNIDEETWYQ
                     LRNTPGVTGFISINNKPVPLGEEEARAILKGMTAEAPKVAVSFALGDSVRIIDGPFAD
                     FRGQIDDINHEKGKIKVLVSFFGRETPVELDFLQAEKET"
     gene            complement(13287..13757)
                     /gene="secE"
                     /locus_tag="VGR22_01400"
     CDS             complement(13287..13757)
                     /gene="secE"
                     /locus_tag="VGR22_01400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEV2127254.1"
                     /translation="MTGDLASQNKGANTLSTEQQAGVKRRSSRAAREGKRRRRNAART
                     AAAPTVQEATQAAIAAGGNEQVAETPASRAEARRQAKAQSAKARSRRGFVSTERTEGL
                     RRFWRETMAEIRKVVWPDAETTRNLTLLVIALSVVLGILLGGIDWVLYQVFEAF"
     gene            complement(13896..13968)
                     /locus_tag="VGR22_01405"
     tRNA            complement(13896..13968)
                     /locus_tag="VGR22_01405"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13933..13935),aa:Trp,seq:cca)
     gene            complement(13987..14157)
                     /gene="rpmG"
                     /locus_tag="VGR22_01410"
     CDS             complement(13987..14157)
                     /gene="rpmG"
                     /locus_tag="VGR22_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV2127255.1"
                     /translation="MARKTKADRPVVTMECTVCRERNYTTEKNRRNDPGRLELKKFCP
                     RCRCHQLHRETR"
     gene            complement(14246..>14376)
                     /gene="tuf"
                     /locus_tag="VGR22_01415"
     CDS             complement(14246..>14376)
                     /gene="tuf"
                     /locus_tag="VGR22_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV2127256.1"
                     /translation="PGDNVQMGVELITPVAIEEGLRFAIREGGRTVGAGVVTSIDE"
BASE COUNT         2583 a         4825 c         4332 g         2636 t
ORIGIN      
        1 taccaagggt ttctccgcgt gctggcgtcc gatcttccga gtggggggaa ggacgtacct
       61 gtggtgcacc attcagttga ctttgcagcg acattcgcgc cgcacgtcag tccgcagttt
      121 agccgccgca tgctcaccaa ggctgccgca ggctttgctg cagccactgc aatggctcga
      181 gatggagtgc cgcgagccga gccggccatc gcacagggtt cggccacccg cgccggtgag
      241 tggtggcaac cagaaggggc tggcagcgac ggggaatata ttgagttcac tgcggacatt
      301 gttttccgag ctctcgcacc gcactgggca ggtgagcctg ccacgcacgg cgccgttgag
      361 atctcggtga gcgccgacgg cgctacctat accgagccgc agatcgtggg cccagccgac
      421 gccgacgccg ggccggccga ccgggatgga cgcacgttcg gccacctgat catgaccgac
      481 ccggcgcagt acgtccgcta ccggacactg gatgcaaacg gcgcgccaac cgtcctcccc
      541 ggcctcgcct tcacctacat cgatgcctcg cacggcccca caggggacga cgtgcatccc
      601 gaagccttcg gcggcgccct caccaagacg ccaatcatca cccgggatcg ctggggtgcc
      661 gcgcaggcgt acggcggctg ggacggcggc actgacggct gggaagccga ctaccgcacc
      721 gtcaagcaca tcatcatcca ccactctgtc acaccgcagt tccgcgatcc gctcgtcgag
      781 atccgctcga tccactacta ccacgcgatc acgcgtggct ggggcgatat cggctacaac
      841 tacctcgtcg acttcctcgg caacgtctat gagggtcgca agggcggcga gaacgtcgtc
      901 ggtgggcacg cgtttcagta cgcctacggc agcgccggca tctgctcgat gggcaacttc
      961 acgctcgaat cctctacgcc cgagtcgatc gccgcgctcg tgtggatcac cgcctgggct
     1021 ggccgtgagc tcgacccgct cggcaaaagc gacttccacg agacaccgaa ctgtccgacc
     1081 atcgccggcc accgcgacgt ctacgacagt acctgcccgg gtgacggcct ctacgccgac
     1141 ctcccctaca tccgcacggc tgtcgccgaa gttctcgctg gcagtcgcaa cgccggcatc
     1201 ccctcgggct acgaccccgg cgacgtcatc cagacgacgg tcagcggcgc caacctgcgc
     1261 agcaagccga gcaccagcgg gtccatcgag gcacggctcg agcgcggcaa ggtcctcacg
     1321 atcattgagg gcccgacgac caacgacggc tacacgtggt accgggtgtc cggcgagcag
     1381 tcggccggct ggctcgccac cacgaccttc agccgttccg acgccgcccc accgcagcgg
     1441 gactatgagc cgaacacgcg cgtggagatt gccgccaacc ttttgaactt acgctcggag
     1501 ccggacctgt gggcatcggt cgttgcacgc ctgccgcagg gcgacacagc taccgtggtc
     1561 gacggtcccg cattaagcgg cggcttgaca tggctgaagc tcgacaccaa cctcggcacc
     1621 ggctgggtag cgcagcagtt cctcgcgccc tcgggccgcg cctggatctc ctcgaagttc
     1681 gccgtgggcg atacggtctg ggtaactaca aacccgctca atatacgcac cgacaccagc
     1741 ctgcaatccc ggatcattgc taggctctgg gagggagaga ccggcgccgt tatcggaggc
     1801 ccgcgtcgtt cggctgggat tatctgggtt cagatccgca cggaccgcgg atccggctgg
     1861 gtatccgagg agttcctgga agaaggttcg gcgcccccac cgcagcccgt caacggcaag
     1921 ttttccgtgg gtgacgaggt cgaggtcgac accgacaagc tcaatgttcg cggtggacca
     1981 gggctcgacg accgtgtggt gaccgttctc tggaccggcg atgaggcgac catcacggat
     2041 ggcgccgtcg aggccgacgg ctacctctgg tacgagatcg acgcggggca tgccagcggc
     2101 tgggtggccg aaacgttcct tgccacggcc ggctccgctg acccacgcgc gagcggcttc
     2161 gaggtcggaa ccaaggtcac ggtgatcgag gattccgtct tcttccggga atccgccgac
     2221 acgactgggc gcatcttctg gaagctctac cggggcgaca ccggcactgt cgcgggtgga
     2281 accgtgcagt ccgatgggta ttcgtgggtg ctgtgcgact tcagcggcac gctcggctgg
     2341 gtcgccacac cgttcctcgg gcggggcacc ggagcccgat cctcctccac cggcctgggg
     2401 gtcggcatca ccgtcaacgt gactgagaac aaggtcaatg tccggccgga acccggcatt
     2461 accgacatcg tgctgggcca gctctacgag ggcgcgacgg cgacgatcca ggagggtccg
     2521 gtggaggccg acggctacac ctggtaccgc atcaccggag cgcgatggga aggctgggtg
     2581 acggacgggt atatcaccac cgccgtcggt ggcagcatcg gtctgagctc gtcggtgcgg
     2641 gtgttcgacg gcgagttgaa catgcgcagt ggtccctcga ccgcagacga cgtcgttcga
     2701 gtgctgccgg acggagcgat cgtcgaggtg ctcgacggtc cggagcgatc agacggccac
     2761 gagtggtatc gggtttccag ctcgcgctac ggcaccggct gggtgatcgc cgcttggctg
     2821 gagcgggtct gatgacgtgt tgcactgaac ggacgccagc ccgtacaatt cctcggattg
     2881 aggcttgcct tcctgggcgt ggcatatcgt cccggggagg ttcagtgcgc gattcaccgc
     2941 cgactatcca caccagaggc aggaaggttt ctacagtcat gaagatcgta ctccggcagg
     3001 atgtgccgaa gctcggtgag gccggcagca tccagaccgt tgccgacgga tacgcccgca
     3061 actacctcat tccgaagggc atggccgtcg tcgccaccga gggcgagatg aagacggctc
     3121 agcacaacat gaaggtgcgc gaacgcaagg tctcgcgcca ggaagagcag ctgcagtcgc
     3181 tggccgacaa gatccagggc acccgactcg agttcaccgc caacgctggt gagcagggcc
     3241 gcctcttcgg ctccatcacg gcgagcgaca tcatggtgca gctcaatgaa aagatcggtg
     3301 agaagatcga tcgccgcaag ctccagctca ccgacccgat ccggacagtc ggcgaacacg
     3361 agctcgagat ccaccttgtc ggcaagctcc gcccgacgat caccgttctg gtccagcctg
     3421 agggcggtct ccagccagag accgacgaga tggctgccga ggccgatgtg ccagaagagc
     3481 tcaaagcgtc ggaggaggat ctcgcgatgg acgaggaagc cgccgccgaa gtcacggacg
     3541 aggaagacga gagcgcgaac gcataacgcg ggcaattcgg tcggccgagg cgacaggctg
     3601 gcggccttgg cgggccccct gccggttcgc ctggctgcca ggacgtagct gaacgcatgg
     3661 acacccaggt cgaccgacta ccgccacaca acatcgaagc cgaacaatcc gtcctcggca
     3721 gcttgctgat cgaccgcgac gcgatcattc gcgtggcatc gtatctcaag gtcgaggact
     3781 tttacgtctc agcgaacgcg accatttact cggcaatccg tgacctctac aaccgccgag
     3841 agccgaccga cttcgtcacc ctctccgacg aactcgagcg tcgcgagatg ctcgatgaca
     3901 tcggcggcgt cggctacctg tcctcgctgc tcagcgcggt cccgacggcc gtccacgtcg
     3961 agtactacgg ccgcatcgtc gagcgcacgg caacccttcg ccgcctgatc gacgccggaa
     4021 cgtcgatcgc cagcatcggc taccaggaca acatcgacac cgaggacgcg ctcgacgcgg
     4081 cggagcgcgc gcttttcaac gtcacccagc aacgatccac taaggacttc cagagcatcg
     4141 atgaggtgat cgaccgcttc ttcgatcagc tcgagttcct gcagaccaac cgcggcgaac
     4201 tcgtgggcgt tccgaccgca ttcaccgacc tcgaccgcct cactggcggc ctccagcgct
     4261 cagacctcgt catcctcgcc gcccgcccga gcatgggcaa gacggcgttc tcgctcggca
     4321 tggcatatgg agccgccgtc aagcacggtc gcaccgtggg catcttcagt ctcgagatgt
     4381 cggccgagca gctggtgcag cgcctgatct cgaccgagac gggcatcgac tcgcaccgca
     4441 ttcggcttgg caacatcagc gaccgggagt gggaagagat ctcccgggcg ttcggtcggc
     4501 tctcggaagc ccgcatcttc atcgacgaca gcgccaacgc cggcatcatg gacgtacggt
     4561 cgaaggcgcg ccgccttcag gccgagcagg gcctcgacct ggtgatcgtg gattacctgc
     4621 agctgatgtc gggccgccgg actgagaacc gggtgcagga gatttcggaa atctcccgcg
     4681 gcctgaaggg cctcgcccgc gagctgaacg tgcctgtcgt ggcgctatct cagctctctc
     4741 gcgcggtgga gtcccgctcc gatcaccgcc cgatgctgtc ggacctccgt gagtcaggat
     4801 cgatcgaaca ggacgccgac atcgtcatgt tcatctaccg tgaggagaag tacgacgagc
     4861 actcggacaa gaagggcatc gccgagatcg tcgtggccaa gcaccgcaat ggcccggtag
     4921 cctcggtcaa cctgcgattc ttcgagtcca ccgcgcggtt tgccgatctg gagatgtacc
     4981 gcgccgactt cgacgaggag taggtgtgcc gacgcgtcgc aggcaggaca acacgccatt
     5041 tgccggcttt gttgacatcg accgcgaagc cgtttcgatt ccgcagcgct ttttcacgga
     5101 ggtgctgccg gagatcacct cgataggaga gctgcgcgtc accctcacct tctttcggat
     5161 gctgaccgac cacggtggtg tcgaagcgcc ggtgcccgag cagacgctcc ttgccgaccg
     5221 gctactgcag caggcgttcc gcgtcgatgg catccctacc ggtgatgtcc gagatgccgc
     5281 gctgaacgcg ctcgatcgcg ctgtggcacg aggcacactc ctccgggttg tctcacgctc
     5341 ggggcgggcg tccacgatgt ggtacttcct gcacacgacg gttacgcgcg agctcgtcgc
     5401 cgccatccag cgcggcgccg tcgccccgcc gcaagtcatg tggtcggaca accatacccc
     5461 ggagatcaac accgacccgc ccacgccgtt ctatctctat gagcagaaca tcggtcccct
     5521 cacaccgatg gtggcggacc gtatctcgcg agcgatggac gactacccgc cagtgtggat
     5581 tgaggacgcg atcgcggaag cggtaacgta caatcgacgt agctggaggt acatcgagcg
     5641 tatcctcgag tcgtggtccg agcagggccg gcccgaccgc tcctcctgac atcatctatc
     5701 gcatcggcag ggattccatc atgaaacgta tcggcgatgt gatgagagag ataccgattc
     5761 cgaagcagcc caccacgggg caggccagcg gcgcggtccg cattcgcaag gacgcggttc
     5821 gatgcccgat ctgcaatgat gcaggctacc tgcgcgcgaa cgtaccggtg ggacacccca
     5881 gcttcggccg cctcttcccc tgcgagtgca aacagcgcga actcgacgag cgccgtgatg
     5941 acgagctccg ccgcctctcc aatctcgatg ccttctcgga ttacaccttc gagagcttcg
     6001 accctgacat ccccggcatg gaggaggcct acgacgtcgc gattgccttc gcccaagagc
     6061 cgaggcacaa ctggctgttt ctctcgggac cttgtggcgt cgggaagacg catctcgcgg
     6121 tcgccatcgc ccggtatgcc atggaatggc acaggatgag cgtctacttc gccgtcgtgc
     6181 cggacctgct cgaccacctg cggtcgacgt tcgatcccaa atccggctcg gcatacgacg
     6241 accgcttcgc cactatcaag aatgcacctt tgctggttct tgacgatctc ggcaccgaga
     6301 acgcaacacc ctgggcgaag gagaagcttt accagatcat taatcaccgc tacagtgagc
     6361 agatgcctac ggtgatcacc acgaatgtcg atctctcgag cagcgggaag atcgacgagc
     6421 gcatctactc gcgcatcatg gaccatcggc tgacgcagca tattgcgatc gacgccgagg
     6481 atttccgcct cgccggcgat ccgcgccgcg tccgccgtac caccggtcgc caccgctgac
     6541 cctcggttcg gctccgggca gccccccaac cgcaaacgat tcccgccctg ccgcacggca
     6601 gggcgattcc atttcgacgg gaaacgcgtg gctcaatcgc ccgcatcggg caagccctgt
     6661 tgcatccggc cgcaactgac ggcactgcag ggcaagcgtg ctcgcgccct gcctcatgtc
     6721 tgcgaatcga agaatctcgc gggcctggga tctccgacga tccttgccgg cgcctgctcc
     6781 tcggttcact cgccgttatg tccggacacc acattcggtc cggcatggcg agtggtagac
     6841 ttcagtgagc gatcacgatg ctccacgtat ctcgccttga ccggtgcacc aatgtcccaa
     6901 atcggttggc tcttgtccca gttgctcaat ccaagggccg tactcgatat cctcgtggtc
     6961 gccctcatca tctactggtt gctgtgggtc gctcagggca cgcgcgcgct ccacgtgatc
     7021 cgcggcatcg tgacgctgtt cgccctgctg gtctttctcg gctgggctct cgatctctcg
     7081 actctcaact ggatcctcaa caactcctgg cccggtctgg tgatcgcgat ccccgtcatc
     7141 ttcgccgccg agctcaggcg cggcttcgaa cagctcggcc atacgggaac atggctgcgg
     7201 ctgcccttca ccgcgaacac tgaggacacc ggtctcgatg aagccgttga cgagatcgtg
     7261 cgcgcttcgg cgcagctctc gcggctccgc tatggtgcac tgaccgtcat tgagcgcgaa
     7321 accgggctgc aggacttcgc cagccgtggt gttcagatcg atggcattct gactcgacag
     7381 ctcctgatca acatcttctt tccgaacacg cccttgcacg acgcagcggt gctcgtgcgc
     7441 aacaatcgca ttctcgccgc cagcgtggtg ctgccgctga ccgacaatat ctcggcgaca
     7501 agcaaccttg gcactcggca ccgtgcggcg atcggcgtca ccgaggagtc ggacgccata
     7561 gccgtagtcg tctcggagga gactggccag atgtccgtcg cacactccgg ccgcctgatc
     7621 cgcaatctgg accaggaccg gctgcggcgc gtgctgcgga cgctcctgag cctcgaccag
     7681 ccgcggcggc gtgaggccgg gtatccgttc ttctccttcg ccaaccggaa cggtgaggac
     7741 cgatcgcaca gttcacgacg caccgagcgg cgccaaaccg gcgactaggc cgccaatcta
     7801 cgattgagtc gaaatgcttc agaacgtccg caatttcctc caggaacacc tgacgggcca
     7861 gcagatggcc cgattcgcgc tttcgctgct ggtcgcactc gtgctctggg gctgggttac
     7921 gcagctgcag aaccccgtgg aggcccagcg gtacgccgag cttgaaatcg taccccccga
     7981 gttgccggga cctctcgaga tcgtgacgaa cctaccgcgg gtgactgttt ccatcacggg
     8041 cgtgtcgtca caattggaag agatttcccg cgccgacatc actgtcacgg tcgatccatc
     8101 gcgagtcgac ggctccggca tgtatcagct tcccgtggtc gtcgagctgg acggcgaggt
     8161 ccgagagatg cgtgtctcac cggacaccgt ctccgtcgag gtcgaggagc tggtgagccg
     8221 gaacttcccg ctcacggtgg agaatcaggc acccgacgag gagtcgggcc ggattgtgga
     8281 taccgatcca gaggtttcgg aagtcaccat cacggggcca gcacctgccg tcgaccgcat
     8341 cgatcgaatc gtgctgcccg tatcggccca aggccacgcg cgcgacttcg tcgaggtgat
     8401 cgagccgttt gcggtcgatg agaacgggca gcgagtccag gaggtcataa ttacccctgg
     8461 gaacgtccgc accgtggtcg agttggaagc gcgtggaaag gctgtcagtg tcgtcccgca
     8521 ggtggtgggc tcccccgccg agcggtacgt cgtgcagcag cagatagcgg ttccatcgat
     8581 ggtcatcgtc gacggacctc ccgaggtgct cgactccctg ctcttcgtca acaccgagcc
     8641 ggtggacatc tccggcgcga ccgactccct gagtgaggtc gtgcctttgg aaaacctgcc
     8701 agagggagcg acgctggtcg agcccgagga gaaccggatc gaggtacggg tctcaatcgg
     8761 gccgtctgga ggaacagcca acaggatctc caacatgcca gtagccgttg aagacatccg
     8821 ggacggcctc gacgcgaccg ttgaccccgg cgccgtcgcc atcgaggtgc aagcaccatc
     8881 cgatgtgctg acctcgctta cgccggacga tgtttcggtt tggatcgatg cgagcggtct
     8941 cgagcctgga atctacgccc tgcaaccaca ggtggaggta ccggacgacg ttgaggtcat
     9001 ccagacggag ccggaagagg tcgtggtagt gatcatcacc aacgggtcag cgaccccagt
     9061 ggcgccagca ggccttcgcg gccaccgcgc gctcactgtc aacgagtaca tggtcgacag
     9121 ctgacgagaa tgacagtgcg gccccgggaa ttcccggggc cgcactgtca cgagcatgtt
     9181 gttcgccgga gcgggtgatc agctcatgcc ggattcgtcg tcatcggacc ccgtcgaaac
     9241 agagtactcc agatcgtgcg caagcagtat cgcctccgcg acctccttca tcgacttgcg
     9301 cgcgtccata ctggtcttgc ggatgcgatg gaacgcctcg ctctcacgca tgccgtgggt
     9361 ctccatcaga acgcccttgg ctcgctcgat caccttgcgg gtctccagcg catccttgag
     9421 gctccccacc tctcgctcca gcgtccggaa ctcctcaaat ctcgcgcggg cgagttcgat
     9481 gaccggcatg agctccgcct cccggaacgg cttgaccacg taccctacca cgccagccat
     9541 ccgggcccgc tcgacgagcc cctggtcgga ataggcggta agcagcacca ccggcgcgat
     9601 gcgctcacgg gtcaattcct cggcggcagc gatgccgtcc acctcgggca tcttgacatc
     9661 catgatgagc aggtctggcc gcagcttgcg cgcaaggtca actgccgacc gcccgtcgct
     9721 ggcctcagcg atcacatcgt agccgagatg cgtcagcatc tcacgaagat cgagacggat
     9781 cagggactca tcatcggcaa tgatgagtcg ccacttctcc gctgatcgac tcgagcgatt
     9841 catgctccat gatcctgtcc gtagacacgc cattgccggc tccaggcctg caatcctgca
     9901 gatcgcctac agccccgcca acgcctgcac tcacaggggg aaaagaactt cgtcggggcg
     9961 agaggattcg aacctccgac caccggtacc ccataccggt gcgctaccgg actgcgccac
    10021 gccccgtcat tcaccaccac agtatacaaa agaggatggg atggcagcaa acgccatccc
    10081 accccaacgc gccgacgcac ccgccgcaaa atcggggcgc accattccga gaagttgcta
    10141 cttgacgtcg accttggcgc cagcatcctc gagcttcgcc ttggcagcgt cggcctcttc
    10201 ccgggcaacc ccctccttga cggccttagg cgccgagtcg accagttcct tggcctcctt
    10261 gaggccaagg ctcgtgatct cgcggacggc cttgatcacc tggatcttgt tgccgcccac
    10321 atcggtgagc acaacgtcga actcggtctg ctcttcctca gcggcagcac cgccggcagc
    10381 gccgggcgca cccgcgacgg cgcccgcagc gatcggagcc gcggccgaga cgccccactt
    10441 ttcctcgagc tccttgacga gctcgttgag ctggataacg gtcatgttct caagttgttc
    10501 gatcagcgcc tcgtgagcca tggtcatggt tcctttcaac ttctatatgc cttggtcggc
    10561 actgacatgt gcctggcggt tgcgtgatat gaaaaactag tctgcggcct gcgcctcgcc
    10621 gccctcgccg cctacggact cggcccgggc attgaacagg taagcgatcg aacgcgacgg
    10681 accgttcagg acgccgacgg cgcgtgccat cggcgatgcg agcatgccga ccagcttgcc
    10741 gagcagctcc tcacgcggcg gcatgctggc gagcacatcg atgtcggatg ttgtaagggt
    10801 ctgcccctcc atcatgccga ccttgagctc aagcacacca cggctcgccc tggcgtagtc
    10861 attgagcgcc ttcgccggag caacgacatc ctcgtatgcg aacatcagcg cggtcggacc
    10921 ctcgagatgt tctcccggaa tgtccacgcc cacgcgctcc gcggcaatcc gtgtcagcgt
    10981 gttcttggca accgtaaact ccgcgttgct gtcgcgcagc ccgcgccgca ggtcctgcag
    11041 ctgggtcacc gtgagccctc ggtagctcgc cacgacggtc agctgggagc gctcgagact
    11101 ctcggcgatc tcatcgattt gcttcgcctt ctgtgctgtt ggcatcggtc atcacctcct
    11161 ttctgattgg atttgtggag caggtgcctg ctccgattca caccaaagaa aatcgtcctg
    11221 ggcagacacg cccaggacga ttgcaccaca ttcggtgatt gcgacctgag cctcggctgg
    11281 aaattaagca aacgcgccag ctgtctttgg ccaggattct tctcttgttg gcgtcactgt
    11341 ttaggacaac tgcgatgcca ggttgatcgt gtcgttgacg tcgaggttca tccccgggtt
    11401 catcgtcgtt gtcagggaga gtttacggca gtagatgccc ttcacgccac tcggcttctc
    11461 gcggttgagc gcatctacga acgcaaggat gttctcgcgc aactgctccg gtgtgaaact
    11521 ccgctttccg agcacggtgt gcacgttccc ggtccggtcg ttacggaact caacacggcc
    11581 gcccttgagg tcgcggatca cgcccgggag atcctcgagc tcgcgcacca ccgtgccgga
    11641 gcggggattg ggcatgagac cgcggcggcc gaggatgcgg ccgagtccgc caaccttgct
    11701 catctgatcc gccatcgcca cggcggcgtc gaatgcgagc catccgttgt tgatctgctg
    11761 gacgtactcg tccgtgccgg cgtagtcggc accggcgtcg agcgcggcac gctcggcctc
    11821 gcctgcggca aaaacgagca cccgaacatt cttgcccgtt ccatgcggga gcgtgacgct
    11881 ggaacgaacc gcctggtcag cctgacgagg atcgatccca agccgcgcat gcagctcgac
    11941 cgtctcgtca aacttcggcg ccgggaactt cccgagcagc tcgatggcct catccagggt
    12001 gtactggcgg tcggcctcga ccagctcggc agcctgctgg tatcgcttgc catgctttgg
    12061 cattacgcgt tctccttcgt ggtgcgatcg ggttgcccct cccacgtgtt atcgcgacgc
    12121 gttgcgtcgt ctatcctacg acctcgatgc ccatggaccg cgcagtgccg gccacctgct
    12181 tcatggcgcc gtcgacatcg atggcgttga ggtcctgcat cttcagctcc gcgatctcgc
    12241 ggacctggtc ctgcgtcacc ttgccgacct tggtggtctt ggggttatcg gtgcccttct
    12301 cgatgcccgc cgccttgcgg agcaggtccg ccgccggcgg ggtcttggtg atgaacgtga
    12361 aggagcggtc ctcgtacacc gtgatcacga ccgggacgat ctgtcccgcc atgctctggg
    12421 tgcgctcgtt gtactccttg cagaagttca tgatcgccac gccgtgctga cccaaggccg
    12481 ggccgatcgg cggcgcaggt gttgccttgc ccgccggaat ctgaagcttg acgtagccgc
    12541 gaatcttctt ggccaactga gtgttccttt cgatacgctc cgggaatcca ccggcgaccg
    12601 acgccgccct gctcccacag acaacctgac agtactacca caggaccaga tctacgtttc
    12661 cttctcggcc tgcaggaaat ccagctccac gggcgtctcg cgcccgaaga acgagaccag
    12721 gaccttgatc ttgcccttct cgtggttgat atcgtcgatc tgtccacgga agtctgcaaa
    12781 cggcccgtca atgatacgaa cgctatctcc gagcgcgaac gagacggcca ccttcggcgc
    12841 ctcggccgtc atgcccttga gaatggcgcg cgcctcctct tcgccgagcg ggacgggttt
    12901 gttgttgatg ctgatgaatc ctgtaacgcc cggcgtgttg cggagctggt accaggtctc
    12961 ctcgtcgata ttcatccgaa cgaggacgta ccccggaaac accttcttct ggacagtgtg
    13021 tcgctgcccg ttacggattt cgatctcttc ctgagtggga acgacgatct cgaagactcg
    13081 gtcctcgagg tcgtaggcct cgacccgact ctcgatggtc ttcttgacct tgttctcata
    13141 tccagagtac gtgtgcagca cgtaccactg cccttgctct gactccgcct cgacaccggc
    13201 ctcttctgca cggcgtgcgg ccaggcgttc acggagggtc tggcgtgcca tcgggcacct
    13261 ccttctgtag ggaattctgc ctatgcctag aacgcctcga agacctggta caggacccag
    13321 tcaatcccgc ccagcagaat acccagaacc accgacagcg caatgacgag gagtgtgaga
    13381 ttgcgcgtgg tctcggcatc gggccagaca accttgcgga tctccgccat cgtctcacgc
    13441 cagaatcggc gaagcccttc agtccgctca gtggaaacaa aaccgcggcg cgacctcgcc
    13501 ttcgccgact gggccttggc ctgccggcgc gcctctgcgc gggaggcagg agtttcagcc
    13561 acctgctcgt tgccccctgc cgcgatggcg gcctgggtgg cctcctgcac cgtcggtgct
    13621 gccgcggtgc gggccgcatt gcgacgccgg cgcttgccct cgcgtgcggc acggctcgaa
    13681 cgacgcttca ctccagcttg ctgttctgta ctcaacgtat tggccccctt gttctgggat
    13741 gccaggtctc cggtcattcg gacaagatcg gagaaggctc ttcgatgcgg ctataccgag
    13801 ggaacggtcg gcatgcgacg tggctggacc ccaaacactg aaatggggcg tgccgctcgg
    13861 ccacacccca cctcgaatcc agcaacatta ccgggcaggg ccggcaggaa tcgaacccgc
    13921 aacctgcggt tttggagacc gctgctctac caattgagct acgaccctaa acgctctgcc
    13981 gaacgcttac cgcgtctcgc gatgaagctg gtggcatcgg caccgtgggc agaacttctt
    14041 cagctccagc cgaccgggat cgttccgacg gttcttctcc gtcgtatagt tacgctctcg
    14101 gcacaccgtg cactccatcg taaccaccgg gcggtcagcc ttggtcttcc tggccattat
    14161 acatcatcct cttgccgcga tcacgctccg acagttcagg atggaggcag gtgccacagc
    14221 actcgcctcc cagcgctcgc cgctgttact cgtcgatgga ggtcaccacg ccggcgccca
    14281 ccgtgcgccc gccctcgcgg atcgcaaagc gcagcccctc ctcgatcgcc accggggtga
    14341 tcagctccac ccccatctgc acgttgtcgc ccggca
//
LOCUS       DASYWL010000038         3121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_282982, whole genome shotgun sequence.
ACCESSION   DASYWL010000038 DASYWL010000000
VERSION     DASYWL010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282982"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..1773
                     /gene="nadB"
                     /locus_tag="VGR22_01420"
     CDS             193..1773
                     /gene="nadB"
                     /locus_tag="VGR22_01420"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEV2127257.1"
                     /translation="MIGAGIAGLSFALSLPFPARVMLVTKGALGESNTRYAQGGIAAA
                     IADSDSPALHLQDTIAAGARLVDVEAARMMVAGGKEAVEWLVANGASFDREDSHFALG
                     KEGAHSRSRVLHAGGDATGAEIERALVARVAARDSLTVHEHATAIELHMDDGRCTGAT
                     LAIGTERRCSVASATTVLAMGGAGRLWAVSSNPVGATGDGIALAMAAGATLSDLEFTQ
                     FHPTVLDLPGEEPFLISEAVRGEGAWLRNDAGQRFMLSIDDRAELAPRDVVARAIHQQ
                     NAAGSRAWLDLRHLDKDLVRLRFPTIARHLGMLGLDLARDLIPVAPATHYFMGGVAAS
                     PTGKTSVPGLLAIGEVSCTGVHGANRLASNSLLEGLVFGRMAARALRPADLSVVIGAP
                     VAPTEVPRPESEATARVVLQRRVQATMRNHVSVVRTAGSLEAAIRELEAILDAPELIH
                     DASLEAASTRNIAVVAREISASALNRHESRGGHYRVDFPLSDPRLDGQHQLVTPVPEG
                     IPTRRFGALDEAWSMVTQ"
     gene            2071..2613
                     /locus_tag="VGR22_01425"
     CDS             2071..2613
                     /locus_tag="VGR22_01425"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2127258.1"
                     /translation="MAASGDGRALEELYERYSRIVMSFSMRMLSERSAAEELTQEVFF
                     RAWKQADSYTSSRGSLATWILSITRNMAIDELRKRQRRPQRADLDDPVTAIANVEDTE
                     TSVEEHAWLQVIRSEMQAALAELPDSQREPIELAYFRGFTQREIAEATGAPLGTIKTR
                     MRLGMRKLREQMEERKVELE"
     gene            2610..>3121
                     /locus_tag="VGR22_01430"
     CDS             2610..>3121
                     /locus_tag="VGR22_01430"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEV2127259.1"
                     /translation="MTTHRHHKLNSDWGCAQIQDEIEAWSIGALDPAEAAAVERHLAM
                     CRDSRHEATRWREVAGMLPFTLEPMNPSGSTKRVLMARIAEDQMKAHQPDLVPRLSAP
                     SQPNGSATPAMQRRFHWSQVLITPLAIALVVMTLWSFELRGQLDSVEAGGAEGTSASV
                     PDSLQSFQMQS"
BASE COUNT          557 a         1047 c          991 g          526 t
ORIGIN      
        1 attcgcgtat acttccggct caccgcgcgg gctgtgagag aacgtgactc tgcctgactt
       61 cgtgtcagtg atacactaag tccgagtgat tcacctgttg cggatcgcgg cagtgacgtc
      121 ggagcgacgg tcatcgacac gcccacctcg caaattgcgc tcatatcggg caccgctcac
      181 accgacatcc tcgtgattgg tgccggcatc gccggcctca gcttcgcgct cagcctcccc
      241 tttcccgccc gcgtcatgct ggtcacgaag ggtgccctcg gcgagagcaa cactcgctat
      301 gcgcagggcg gtattgccgc ggcgatcgcc gatagcgact ctccagccct ccacctgcaa
      361 gacacgatcg ctgccggcgc caggctcgtc gacgtcgaag ccgcccggat gatggtcgcc
      421 ggcggcaagg aggcggttga gtggctggtg gcgaacgggg ccagcttcga ccgagaggac
      481 agccactttg cgctcggcaa ggagggcgca cacagccgct ctcgggtgtt gcatgcgggc
      541 ggcgacgcca ccggtgccga gatcgagcgg gcactggtcg ccagagtcgc cgcgcgcgac
      601 agcctgacgg tacacgaaca tgccaccgcg atagaattgc atatggatga cggtcgctgc
      661 accggcgcga ctctcgccat cgggacggag cgccggtgca gcgtcgcgag cgcgaccacg
      721 gtgctggcca tgggcggtgc aggtcggctc tgggccgtct cctccaatcc ggtgggtgcc
      781 acgggcgacg gtatcgcgct cgccatggcc gccggggcga cactgtccga cctcgaattc
      841 acgcagtttc acccgaccgt gctggatctc cccggcgagg agccattcct catctccgag
      901 gcggtccgcg gcgaaggggc atggctccgc aacgatgccg gtcagcgatt catgctgtcc
      961 atcgacgatc gtgccgagct cgctcctcgc gacgtggtcg cccgagcgat ccatcagcag
     1021 aacgcagcag gcagccgggc ctggcttgat ctgcgccatc tcgacaagga tctggtgcgt
     1081 ttgcgctttc cgaccatcgc ccgtcacctc ggtatgctcg ggctcgacct ggcgcgcgat
     1141 ctcattcccg tcgcgccggc gacccactac ttcatggggg gtgtggctgc gtcgccgacc
     1201 ggcaagacct ccgttccggg cctcctcgcg atcggtgagg taagctgcac cggcgtacac
     1261 ggtgcaaacc gcctggccag caactccctg ctggaggggc tggtcttcgg tcgcatggca
     1321 gcgcgggcac ttcgcccggc ggacctgtca gttgtcatcg gggcaccggt ggctccgact
     1381 gaagtccctc ggcctgaaag cgaggcgaca gcgcgggttg tgctccagcg ccgcgtgcag
     1441 gcgacaatgc gcaaccacgt atcggtcgtt cgaacagcag gatcccttga agctgccatt
     1501 cgtgagctcg aagcgatcct cgacgcgccc gaactcatcc acgatgcctc gctggaggcg
     1561 gccagtaccc gaaacattgc cgtggttgca cgcgagattt ccgcttcggc cctcaatcgc
     1621 cacgagagtc gcggcgggca ctaccgcgtc gacttccctc tttccgaccc caggctcgac
     1681 ggccagcacc agctcgtcac accagtgccc gaagggatcc cgacgcgccg cttcggcgcg
     1741 cttgacgagg cgtggtctat ggtcacccag tgatggagtc tccagcaccc gggagctgcg
     1801 tggtatgttc atgctggcgg tgaggccgct gcccatccat tggactgcct gctcccgcct
     1861 ccatcgacat tcccatcgtg gcgccccccg cccggctgaa ccatcgaagc tacacaccat
     1921 gcaaccaaac gtaccggacc ggcgtacatc tcaacagccg accctaatcg gcgcccgcgg
     1981 cccgaagaca gaaaggcgag gcccgtgacg cctgctgaca ctgttctgcg ccgcccgctg
     2041 gccgacgtcg acgatgcggg tctatgcgcc atggcggcat ccggggatgg tcgggcactg
     2101 gaagagctgt acgagcgcta ctcgcgaatc gtaatgtcgt tctccatgcg gatgttgtcg
     2161 gagcgctcgg cggcggagga gctgacccag gaagtatttt tccgcgcctg gaagcaggca
     2221 gacagctaca cctcctcccg cggctcgctc gctacctgga tcctgagcat cacgcgcaac
     2281 atggcgatcg acgagctacg aaagcgacag cgccgtcccc agcgcgccga tctcgatgac
     2341 cccgtcacgg cgatagcgaa cgttgaagat accgagactt ccgtggaaga acatgcgtgg
     2401 ctccaggtga tacgaagcga gatgcaggca gccctggccg agctgccaga cagccagcgc
     2461 gagccgatcg agttggctta cttccgggga ttcacccagc gcgagatcgc agaggccacg
     2521 ggtgcccccc tcggcacgat caagacccgc atgcgactgg gaatgcgcaa gctccgggaa
     2581 cagatggaag aacgaaaggt ggagttggaa tgacaacgca tcgccaccac aagctgaata
     2641 gcgactgggg ctgcgcgcag atccaggacg aaatcgaagc atggtccatt ggtgcgcttg
     2701 acccggcgga ggccgccgca gtcgagcggc acctcgcaat gtgtcgggac agccgccatg
     2761 aagcgacgcg ctggcgggaa gtcgccggta tgctgccctt cactcttgag ccgatgaacc
     2821 cgtcgggctc gaccaagcgg gtcctcatgg cccgaatcgc cgaggaccag atgaaggcgc
     2881 accagccgga cttggtaccg cgtctttctg caccctcgca accgaacggc tctgcgacac
     2941 ctgctatgca gcgacggttc cactggagcc aagtgctgat cacgccactg gccatcgccc
     3001 tcgtggtgat gaccctctgg tcatttgagc ttcgaggcca actcgacagc gtcgaggcgg
     3061 gtggcgccga ggggacgtcg gcatcggtgc cggacagtct ccagtccttt cagatgcagt
     3121 c
//
LOCUS       DASYWL010000039         1572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_527072, whole genome shotgun sequence.
ACCESSION   DASYWL010000039 DASYWL010000000
VERSION     DASYWL010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527072"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VGR22_01435"
     CDS             <1..497
                     /locus_tag="VGR22_01435"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127260.1"
                     /translation="LAELFSWFSFDREYRYPNHFLLSNRLLRVREKLEDIEHEVIGEE
                     RDHRLFISEGHNPGFYGAAMAWCQGATMAEIGESLELSEGDLVITFNKTIDLLRQVRE
                     MLTDVQPDHPVRIRLSDAIERLRKGIVAQSLTLGFTPIAEFEDEADEEEIVETEEESV
                     LDEE"
     gene            501..1373
                     /locus_tag="VGR22_01440"
     CDS             501..1373
                     /locus_tag="VGR22_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127261.1"
                     /translation="MSEPVFTAARLDRVRAAADRALPFLEQMDDPLGAAWVRHRWKDG
                     PSGDVLAELAAYQQPDGGFAGLEVDIEAPVSTAFDGHLAMHVLLGLRDRLQSELVDRL
                     GGWYARNQHEDGDWHFSPEAYEHRLAPWFASWEFPGLNPACCVAGLTHALGIATPEML
                     VRVERLWREKATIDQARSGDFYTMLPIIEYVAHIDVEDREVWLHAIADGVERGIADSA
                     FGDAEHALDHLAGSGPEVIARLDRDDVVTVIDAVLADQREDGGWPTPYDPAWRPWFTA
                     STLVLLARLHHTIA"
BASE COUNT          273 a          512 c          536 g          251 t
ORIGIN      
        1 agctcgccga gctcttctcg tggttcagct ttgaccgtga gtaccgctac cccaaccact
       61 tcctgctcag caaccggctg ctccgcgttc gagagaagct ggaggacatc gagcacgagg
      121 tgatcggcga ggagcgcgac caccggctct tcatctccga gggccacaac cccggcttct
      181 acggtgcggc gatggcatgg tgccaggggg cgacgatggc ggagatcggc gagtcgctcg
      241 agctctccga aggcgatctg gtcattacct tcaacaagac gatcgacctg ctccggcagg
      301 tgcgggagat gctcaccgac gtccagccgg accacccggt gcgcattcgg ctcagcgacg
      361 cgatcgagcg gctcaggaag ggcatcgtcg cgcaatccct cacgctcggt ttcaccccga
      421 tcgccgagtt cgaggacgaa gccgacgagg aggagatcgt cgagaccgag gaggagagtg
      481 ttctcgatga ggagtagcgt gtgagcgagc cggtcttcac cgcggcgcgt ctcgatcggg
      541 tccgcgctgc ggcggaccgc gccctgccct ttctcgagca gatggacgac ccactcggtg
      601 ccgcatgggt gcggcaccga tggaaagacg gcccctcagg tgatgtgctg gcagaactgg
      661 cggcgtacca gcagccagac ggcgggttcg ccggcctgga ggtcgacatc gaggcgccgg
      721 tcagcacggc gttcgatggc cacctggcaa tgcatgtcct gctcggcctg cgggaccgtc
      781 tgcaatccga actcgtcgac cgcctcggcg ggtggtacgc ccgcaaccag catgaggacg
      841 gtgactggca cttctcgccg gaggcgtacg agcaccgtct cgcgccgtgg tttgccagct
      901 gggagttccc ggggctcaac ccggcctgct gcgtcgccgg tctcacccat gccctcggca
      961 tcgccacacc ggagatgctc gtgcgcgtcg agcggctctg gcgcgagaaa gcgacgatcg
     1021 atcaggcacg ctcgggcgac ttctacacga tgctaccgat catcgaatac gtcgcccaca
     1081 tcgacgtcga ggatcgcgag gtctggctcc acgcgatcgc cgatggggtc gagcgcggca
     1141 tcgccgacag cgcgttcggg gacgccgagc acgcgctgga ccacctcgcc ggcagcggac
     1201 cggaggtaat cgcacggctc gaccgcgatg atgtggtcac ggtgatcgac gccgtgctgg
     1261 cggatcagcg ggaggacggc ggctggccga cgccctacga ccccgcttgg cgcccctggt
     1321 tcaccgccag cacgctcgtc ctgctcgcca ggctccacca cacgatcgcg tagagccgac
     1381 cgggcgatcg cagcgggacc ttcaaacagg aggttcgaca gtgggatacc gagcatgggg
     1441 gcgaagcgga tccggaggtg ttccggcagc agattctgcc caggctccaa catatctccc
     1501 tcggggcgat ggcaaaggcg accggattga gcgagggcta ctgctcgttc attcggcgtg
     1561 gggtaaaggt gc
//
LOCUS       DASYWL010000040         2528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_39184, whole genome shotgun sequence.
ACCESSION   DASYWL010000040 DASYWL010000000
VERSION     DASYWL010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39184"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VGR22_01445"
     CDS             complement(<1..279)
                     /locus_tag="VGR22_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127262.1"
                     /translation="MEAALTTSPIGLRPSSRQTLERSSPELARTLSDLSRYLEEQGLA
                     SRWPFPVGGLHLFLVLRQWVQEDANETLANQMLSNKDWCQAETMLRKRL"
     gene            1013..1780
                     /locus_tag="VGR22_01450"
     CDS             1013..1780
                     /locus_tag="VGR22_01450"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEV2127263.1"
                     /translation="MRLDGKTAFVTGADSGIGRAIAITFAREGADVAVHYHTDRRGAE
                     QAADAVRHHGQRAEIVGADFTDPMAAEGLIAVVADRMGRLDILVNNAGRGSAASASLD
                     ITTDVFLDVLRVDLVAPFVLARDAARLMVEQGGGSIVNVTSVHEQIPSPGGADYSAAK
                     GGLRMVTRTLALELAPPGVRINNLAPGMIATPMTTPALHDPEQSAEATAKIPMGRPGE
                     VQEMANVALFLASDEASYVTGSTFFADGGLRQKVGLA"
     gene            1798..1971
                     /locus_tag="VGR22_01455"
     CDS             1798..1971
                     /locus_tag="VGR22_01455"
                     /inference="COORDINATES: protein motif:HMM:NF024645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEV2127264.1"
                     /translation="MTGPTDADIREAEWLAAQSPSSGPVGNDEELTCPECGGAIGVDD
                     VICPHCGVPLVGG"
BASE COUNT          431 a          784 c          868 g          445 t
ORIGIN      
        1 cagacgcttc ctcaacatag tctccgcttg gcaccagtct ttgttgctca acatctggtt
       61 cgccagcgtc tcgttcgcgt cctcctgcac ccactgccgg agcacaagga agagatgcaa
      121 gcccccgacg gggaatggcc agcgcgacgc gaggccctgt tcctcaagat agcggctcag
      181 gtcggacagg gtacgggcca gttcgggcga ggatctctcg agcgtttgcc tgcttgacgg
      241 ccgcaagcca atcggactgg tggtgagcgc ggcctccacg tctctggcac gatcctcgac
      301 ccagcgccgt accgtcgcct gcatcgtagg cgcacacacg agtgggggcg ggtcgtcagt
      361 gttccgcggg tctagcaacc gacggcacgg gcagccgcca tcatcggcgc gtggacgctc
      421 agagtgcagg tctgtcatcg ccatactcgc tcacgactca gcacgtgccg gcaaactgga
      481 cacggcccga ttctagtcct tcgcgcgctg cctcgaacag agctagccag cgggcgtggg
      541 acggtgagga cggctggctt tgacgctcgg taccacggcg gtggtgatgc ccttgatgag
      601 atgaccgaag tgaagttgct ggcgaagcgg gcctccacct tgcccacccg cgccgatgcg
      661 ggagtggcaa ggcaatcgac gatgattgta gaggctcggt cgtacgcggc ggtgggatag
      721 cgaggcatgc cttcgctgcc atctcgttat cccgatggtt cctgacatgg atgtgggacg
      781 ggtgcgtatc gtgccgctac cgccgggtca agcaaggaat ggtggaggga aattgatctg
      841 caaacccctc actaccgaca agtcgcgatg ccaaagctcg gttcagccgg tttcgtttcg
      901 gaatcgcctc tttggccaca cgtcattgcg agctggtcgg tattttgcgt agcaggtcgc
      961 tcccggcacg accggcgtgg cactgaccga tcaccagaaa aggatcagcc ccatgcgact
     1021 tgacgggaag acggccttcg tgaccggtgc cgacagcggc atcggccggg ctatcgccat
     1081 caccttcgcc cgggaggggg cggatgtcgc cgtccactac cacaccgacc ggcgcggcgc
     1141 cgagcaggcg gcggatgcgg tccggcacca cggccagcga gccgagatcg tcggggccga
     1201 cttcaccgat ccgatggcgg ccgagggact gatcgccgtg gtggcggatc ggatgggtcg
     1261 cctcgacatc ctcgtcaaca acgccgggcg gggatcggcg gcgtcggcct cgctcgacat
     1321 caccaccgac gtgttcctgg acgtgctccg tgtggatctc gtcgccccgt tcgtgctggc
     1381 gcgggacgcc gccaggctga tggtggagca gggcggcggg tcgatcgtca atgtgacctc
     1441 ggtccacgag cagatcccgt cgccgggcgg ggcggactac agcgccgcga aaggcggctt
     1501 gcggatggtg acccgcacgc tggcgctgga gctggcgccg cccggtgtgc gcatcaacaa
     1561 cctcgcgccg gggatgatcg ccacaccgat gacgacgccg gcgctgcatg atccggagca
     1621 gtcggccgag gccacggcga agatcccgat gggtcgcccg ggggaggtgc aggagatggc
     1681 caacgtggcg ctcttcctgg catccgacga ggcgagctac gtcaccggca gcaccttctt
     1741 cgccgacggc gggctgcgcc agaaggtcgg gctggcctga cgctggaggg atggagcatg
     1801 acaggaccga cggacgcgga catccgtgaa gccgaatggc tcgcagcgca atcgccgtcg
     1861 tcgggtccgg tgggcaacga tgaggagctg acctgtcccg agtgcggcgg cgccatcggg
     1921 gtcgacgacg tcatctgccc gcactgcggc gtgccgctcg tcggcggcta gcgtcccggc
     1981 cggcactgtg gcgacgtgtc acgcgttgcg acgcgcgcca gccttggtat gcggctggtg
     2041 gtgctcgacg ttgtgtgtca gtgtgttcgg cggttgcagc gcattccacc ccgtcggatt
     2101 gtgagagggt gcatctgatg gggtacgtcc gagactggat tacgaggtat cgctcgggaa
     2161 cgactgccga atccgggagc gacgatatcc cccatcaacc gcggcaaaga tgcgcgctcg
     2221 gccgccaagc gccctcaaaa tcgaccgagt ttcacgattg gtgccgaacg cgggattccg
     2281 gagccgccat gcgaaccagt accctgcagt gcgacaaaat gcgtaggtat agggttttca
     2341 gctcgttcct ggtctagcgt gtccggcctc gtccccccag tgctggggcg tccgtgacaa
     2401 agttgtgaca cggtcttcag cggttcactc atatagataa gggtaggctg tcgaaggcga
     2461 ggtggtctcg ggagactagt ttagcctcct cgtccccata ggatgcgtca gggctattgg
     2521 aagccacg
//
LOCUS       DASYWL010000041        15054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_414638, whole genome shotgun sequence.
ACCESSION   DASYWL010000041 DASYWL010000000
VERSION     DASYWL010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 15054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15054
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414638"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VGR22_01460"
     CDS             complement(<1..556)
                     /locus_tag="VGR22_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEV2127265.1"
                     /translation="MPHHLDKIRVRGAREHNLKNIDVDIPRDNLVVITGLSGSGKSSL
                     AFDTIFAEGQRRYVESLSAYARQFLGQMEKPDIDHIDGLSPAISIDQKGASRNPRSTV
                     GTVTEIYDYLRLLYARIGHPHCPNCGRPITQQSVQQMADQVLTQPDSTRLMILGPVIR
                     DRKGEHVAVLDEIRRQGFVRVRVDG"
     gene            1092..3779
                     /locus_tag="VGR22_01465"
     CDS             1092..3779
                     /locus_tag="VGR22_01465"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEV2127266.1"
                     /translation="MSASQTSPAVFQTGELAKSYDPAAIEHGIYDWWDRQGYFQPSDD
                     EGQEPFVVVMPPPNLTGVLHVGHALFVALEDIMVRYNRMKGRAALWIPGADHAGIAGQ
                     LTVVRLLAEEGINRHDIGREKFLERTWEYMDALRPRVREQMKSLGASADWTRFAFTMD
                     PGPGRAVRHVFKHLYDKGLIYRGERIISWCPNDRTALSDLEVVYKEQQSNLWSIAYPV
                     EGTEERIVVATTRPETMLGDTGVAVHPEDERYQHLVGKQVRLPLMGRLIPIVADEHVD
                     MTFGTGAVKVTPAHDPNDFEIAKRADLPALMIMHEDGTLNAETGEFAGMMMAEGRKAV
                     VARLEEQGFLVGIEPHEHSVGTCDRCGHVVEPLLSKQWFIKMAPLAKAALDAACSGDV
                     TFVPERYKGVYTNWMENIHEWCISRQLWWGHQIPIWYCDACGDTFASVEESVDTCPSC
                     GGPVHQDPDVLDTWFSSGLWPFSTLGWPEQTEDLRRFYPSDVLETGYEIIFTWVARMI
                     FFGIEIMGEVPFHTVYLHGIVRDDEGAKMSKTKGNVIDPLEVSEEYGADALRYTLVTQ
                     ASPGNDSRLSLQKVEAGRNFGNKLWNMTRFAMRSFDPDVIEVAEDGPARPIGNLQIVD
                     RWIISRLDAVTAGVSGLMERHLYGEAGRQIADFLWSEVADWYIEAAKVRLREEGEAAR
                     MVGQVIVYVLERALRLLHPYMPFITETLWQQLPHAGESVMIAPWPEAGERDMTAERHV
                     GAMMELVRGIRNARTDAGVEPAKWIAAMVYPGEHLEAFEQLRGEIAFLARVQDDQLTF
                     AEGKPEESGKAITVVAGDVMASLPLEGMVDLGVERERLEKELEEARAEQQRAEKQLSN
                     ESFVSRAPEHVVQAQRDRLERAGEQVAVIEQRLSALADA"
     gene            3784..4422
                     /locus_tag="VGR22_01470"
     CDS             3784..4422
                     /locus_tag="VGR22_01470"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEV2127267.1"
                     /translation="MPVDRAWFSRHPVKVAWDLIGCVLRIERDGVVVSGRIVETEAYA
                     GPDDPASHASRSPVGREVMMGRPGTVYTYLSYGIHTMMNIVAHEERQGGAVLLRALEP
                     LEGIEVMGERRNGVSETQLAKGPGSLGQAMDIRLSDLGVDLIDAPEWTLLHGDVRHPV
                     HAGPRVGISKAVVAPWRFFERPNPHVSRHRKGEPVERHELPSLIPPSGTPLE"
     gene            4469..6223
                     /gene="mutL"
                     /locus_tag="VGR22_01475"
     CDS             4469..6223
                     /gene="mutL"
                     /locus_tag="VGR22_01475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEV2127268.1"
                     /translation="MTIRLLTEDTIGKIAAGEVVERPVSVVKELIENAVDAGATRIDV
                     DIAGGGSERIRVSDNGLGMTRSDLELAIQRHATSKLSTFNDLETLSTLGFRGEALPSI
                     GAVSHFMIRTAAAGEARGAQLVSAFGRKAEVAVVAARQGTVVTVEDLFGNVPARRKFL
                     RQPGTEATYISRLVSAYAANWSDIAWSLTSEARRSISTQGNGDDIQAAIGIYGTELVD
                     AVLPLSSDAEGARVEGIEVSGWILAPRVSRSHRQNLFFFVNGRLIQHRSLVYALEEAY
                     HSLLMVGRHPVGMVRIDLDPRAVDVNVHPTKAEVRFADERAVARAVQRAAHAALTMQQ
                     QDVLPEVAYECPQGPPSVRQTFFEHQQQAPQPGGATFARRRADEDDEPDQAPSPGLQH
                     RSGVPALRVLGQVGGAYIIAEGPGGIFLIDQHAAHERVMYEKILGQVETKDIDRQPLL
                     DPLVVDLAPHELTVLERSMDELREIGFDLEMFGEQSVIVRSVPAVMQRVDIRERIHLI
                     LEELAEGGSGDSWFDSVAISAACHTSIRAGQALSLDEMRELVAQLERTRQPRACGHGR
                     PTMLHMSQADLEKQFSRR"
     gene            complement(6255..7088)
                     /locus_tag="VGR22_01480"
     CDS             complement(6255..7088)
                     /locus_tag="VGR22_01480"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HEV2127269.1"
                     /translation="MESTTTPSSGTPSLPGVSIVIPVYNESERIGSTLEQLAEFLRAA
                     PFRAEVIIADDGSTDDTRGIAARHAGSYPHFHLLTLPHGGKARAVLAGLRVATQPIVG
                     FMDADLATPLPTLVEAVELIQGGADVAIGSREGEGSRRIGEPEYRHIMGRLFNRLVRL
                     LILPGIHDTQCGFKFMTSEAATEILSRVQLYRNSEEVSHPRVTAFDVELLFIARQLGL
                     AIAVIPVTWSYGTSSKVSPLRDTLQNLRDVVQVWLNGRRGLYAGDATPLAGRCDETAN
                     S"
     gene            7174..7554
                     /locus_tag="VGR22_01485"
     CDS             7174..7554
                     /locus_tag="VGR22_01485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEV2127270.1"
                     /translation="MKPILYLYRSCTSCRNAASILDAHGVDYDVREYFKEPFTREELE
                     SVLERAGQKPSDLVAARSVPYRAQGLAERGLSEDELLERMLAEPRLVRRPILVTEDEV
                     AIGFDRDRYEQKAKTLASRVSADA"
     gene            7582..8457
                     /locus_tag="VGR22_01490"
     CDS             7582..8457
                     /locus_tag="VGR22_01490"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEV2127271.1"
                     /translation="MSLAFGAHVSVSGGVDKAFARAEEFQMDGIQIFSKNERQWRAKP
                     LADDVIERWFDERERTGISKIVAHASYLINLATPREDLREKSLAAFLEELDRCEMLDI
                     PSLVIHPGAHTGSGEDVGVKAVADALNQVHELRPDHKVRTLLETTAGQGTTLGRTFEE
                     LAAIIDQVEAKERVMVCLDTCHVFAAGYDIRRPESYAAVMLHFAHTIGIDRLQAIHLN
                     DSQNGPGTNKDRHEHIGQGEIGLEGFRNIVNDPRLIGVPGLLETEKGENNVEDGRNLA
                     RLRDLAERPVGTTAE"
     gene            complement(8657..9163)
                     /locus_tag="VGR22_01495"
     CDS             complement(8657..9163)
                     /locus_tag="VGR22_01495"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HEV2127272.1"
                     /translation="MFDEVKGFAVTLKRLASPKVTTEYPEEKRPMEPRFRGLPSLRTD
                     PATGEPLCVACGLCARICPTTCLEMNIVASEEGDRELGEFVLKAGRCMFCGRCAQVCP
                     VDAITMSHEYELSVLERDGLIYTKTELAQIGAQMPTWWESGIGLDHQNTNAPTKYDRF
                     RDVASELY"
     gene            complement(9156..11630)
                     /locus_tag="VGR22_01500"
     CDS             complement(9156..11630)
                     /locus_tag="VGR22_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEV2127273.1"
                     /translation="MVAATEQQMVTGLINGQEVTVPQGTTILEASRIAGIEVPNLCYQ
                     PLLRPWGSCRICTVEVLGKRGGLVESCAAVFQDGAEVLTHSPDSMRARQYILQMYLID
                     HALDCPTCDKSGECYLQDNTYLHNINANPYRRPKLAQPYEHFSDTVDYKWDRCIMCNR
                     CTRVCDEMIGVIAIEQSNRSLEATITTSFGRSLEENTCTNCGMCIAVCPVGALTDRHF
                     GHHPWELDTTETICGFCDVGCTLNIESHKGIVRRTTHLWERGVNHGYTCQYGRWGHEV
                     VQGPDRLYHARVRGEDGRSYEIDIDEAVDEIAENLAHYQGSSFAALASAENTNEEAYV
                     LQQFSRAVMQSNNVDRLMSPSQLDVERSVRDALGLSVSNTNNQQEFFTHVKNVMVIGP
                     SVFKAAPVFSYWVNHSRLYREARIVVISTEHYLLCDRAALWLQPKPGTTHKVVDAIAA
                     AIVALNLESPGDADVTALVKRLEGIDLNAVAGETGVASDDIVQAARIFVTGFLEESDR
                     AAKPGELPGTIIQTVAHDIIQSIAYDWGIEGMQDYPDAGSIVDACTNLSILTGNLGKA
                     GAGIATPRGPANYQGTTDMGMVPDMLPGGVPVSDESARVSLERTWNSRWAGRVATSNG
                     YVQQQELPSEPGIGMEGLAEAIESGRVKAMWVEGGARTRTFFPNARLFEALQKLEYLV
                     VADIYDSPLAAIADVVLPVSANLEKDGTFTSFDRVVQRVRAAVPALGEAQSSLEYLTE
                     IAARFGYRLEYGHPSHVMREIATVVPGYNGMSYAFLEREGVVTPTAGFGGDGVHVLDR
                     TTGLTPTVMGQQRRGHRPPEKESADV"
     gene            11978..13450
                     /gene="gatB"
                     /locus_tag="VGR22_01505"
     CDS             11978..13450
                     /gene="gatB"
                     /locus_tag="VGR22_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEV2127274.1"
                     /translation="MAAQAIGRTYRTVIGLEVHAQVLTGSKMYCPCSASYADAAPNTH
                     VCPVCLGLPGALPVINRAAVEMVIHTGLALNCEIASDSKMDRKNYVYPDLPKGYQISQ
                     YDLPLCLDGALTFEVDGELRTSGITRVHIEEDTGRLVHDQSDEDDSLVDLNRSGVPLM
                     EIVGEPDLQSPEEAREYLMALRQILRYVGASTGNMEEGAFRCDANISTRSADGEFTGP
                     KVEIKNMNSFRAVERALRFEEQRQREAAERGGELVQETRGWVDAQGITVSQRTKEQAH
                     DYRYFPEPDLPPLRMKKTDIDRIRESLPELPAQRRRRLMDEFGLPPGDAVTLTMERPI
                     ADLYEDVVAATDARFHRAVANWIVNDIMGLARARGMAQDQLPLSAAQIRDLVRGVENG
                     KLTARAAKDLLPQIEDGEMPMDAAERFNLLSLDDADVVTQAAAEAIEANPAVVQDYLG
                     GKKAAVGRLIGETMKRTGGRAKPDVVRETLVKLLSQVEGE"
     gene            13507..14805
                     /locus_tag="VGR22_01510"
     CDS             13507..14805
                     /locus_tag="VGR22_01510"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEV2127275.1"
                     /translation="MVTATGIREAATAPEPTEDEDIFDVAVRQFHIAADVVNLDDGIR
                     AVLSDCERELTVHFPVEMDDGSVQTFTGYRVQHNSGPGPTKGGIRYHQAVTRSEVKAL
                     AMWMTWKCAVVGLPFGGAKGGVRVNPKMLSANEIQNLTRRYTTEIQPLIGPTRDIPAP
                     DVNTNAQVMAWLMDTYSMNIGYSERGVTTGKPIVLGGSAGREEATGRGTVFAVEDGAR
                     HVGLDLSSARVAIQGYGNVGAVAGRLMVQRNKSTVIAVSDSSGGIYNPNGLDLDEVDR
                     AKRRTGTVQGYADADKVTNSELLELPCDVLIPAALEGQITGRNADRIKAGIIAEAANG
                     PTLPAADDILYERGVLVIPDIYANAGGVTVSYFEWVQALQAFPWTLDQVNERLRQIMQ
                     DSFTHVLAKSTEYGVHMRTAAMIRAIERVSEFTRLRGIYP"
BASE COUNT         2675 a         4628 c         5146 g         2605 t
ORIGIN      
        1 cgccgtcgac ccgcacgcgg acgaaaccct gccggcgaat ctcgtccagc acggcgacgt
       61 gctcgccctt gcgatcgcgg atcactgggc cgaggatcat cagccgtgtg ctatccggct
      121 gggtgagcac ctgatcggcc atctgctgaa ccgactgctg tgtgatgggg cgaccgcagt
      181 ttgggcaatg cgggtgcccg atgcgcgcgt agagcagtcg aaggtagtcg tagatctcgg
      241 tgacggtccc gacggttgag cggggattgc gggaggcgcc cttctggtcg atcgagatcg
      301 ccggggagag accatcgatg tggtcaatgt ccggcttctc catctggccc aagaactgac
      361 gcgcgtaagc ggagagggat tcgacgtagc ggcgctggcc ctcggcgaag atggtgtcga
      421 aggcgagcga cgacttgccc gagccggaga gtccggtgat gacgaccagg ttgtcgcggg
      481 ggatgtcgac gtcgatgttc ttgaggttgt gctcgcgggc gccgcgcacg cggatcttgt
      541 caaggtgatg cggcatcagg gggacctccg gcgaatgctg ctcgctcgga aaccggttgg
      601 gaaccagtcg tgatctgttc gggagagacg accccatgcc ccaacaatcc gaacatgtga
      661 tcgcatctat cgattgtacc acgcctgacc gtacactccc ggggcccgcg tacctttccg
      721 ccgtgctggg actgaacagg ggacctcaat gagggtgtgc gtggtgaggc aagcgtgcca
      781 gtatggcgta tacttgacga aacgccgaag atcgggagga gtaggagcgt atctctccca
      841 gggagccgat ggccgttcga ctgcgagccg tggcagagct cggcgctccc aacgtcgccc
      901 gggaggccca gcggcgatcg cggcaatgcg tgagtcgatg gcggtaacgc ccgttatcgc
      961 gtgacgagtg cggcagcctc tgtctgccga agcgaggtgg taccgcgggc cttcccgtcc
     1021 tggcagcagg gcgggctttt tgtgtttgaa caggcgctgc agaggcgatg agaaagagac
     1081 acgaggcaac gatgagcgca tcacagacat cgccggccgt tttccaaacc ggcgagttgg
     1141 cgaagagtta cgacccggcg gccattgagc acgggatcta cgactggtgg gatcggcagg
     1201 ggtacttcca gccctccgac gacgagggcc aggagccgtt cgtcgtcgtg atgccaccgc
     1261 ccaacctcac cggtgtgctg cacgtcggcc acgcgctctt cgtcgcactc gaggacatca
     1321 tggtgcgcta caaccggatg aagggacgcg cggcgctgtg gattccgggg gcggatcacg
     1381 ccggcatcgc cggacagctc acggtggtgc gccttctcgc cgaggagggc atcaaccggc
     1441 acgatatcgg ccgcgagaag ttccttgagc gcacctggga gtacatggac gcgctcaggc
     1501 cgcgcgttcg ggagcagatg aaatcactcg gcgcctcagc cgactggacg cgcttcgcct
     1561 tcacgatgga cccgggcccc ggccgcgcgg ttcgacacgt gttcaagcac ctctacgaca
     1621 aggggctgat ctaccgaggc gagcgcatca tctcctggtg tccgaacgac cgcacggcgc
     1681 tctccgacct cgaggtcgtg tacaaggagc agcagtcgaa cctctggagc atcgcctacc
     1741 cggttgaggg cacggaggag cgcatcgtgg tcgccacgac acgaccggag acgatgctcg
     1801 gtgacaccgg cgtggcggtc caccccgagg atgagcgcta ccagcacctt gtcggcaagc
     1861 aggtccggct gccgctgatg ggtcgcctca tcccgatcgt ggccgacgag cacgtcgaca
     1921 tgaccttcgg caccggtgcg gtgaaggtga caccggccca cgaccccaac gacttcgaga
     1981 tcgccaagcg tgctgatctg cctgcgctca tgatcatgca cgaggacggc accctcaacg
     2041 ccgagaccgg cgagttcgcg ggtatgatga tggcggaagg tcgcaaggca gtcgtcgcca
     2101 ggctcgagga gcaagggttc ctggttggca tcgagccgca cgagcacagc gtcggcacct
     2161 gcgatcgttg cggccatgtg gttgagccgc tgctgagcaa gcagtggttc atcaagatgg
     2221 cgccgctggc gaaggcggcg ctcgatgcag cctgtagcgg cgatgtcacc ttcgtgccgg
     2281 agcggtacaa gggcgtctac accaactgga tggagaacat ccacgagtgg tgtatctctc
     2341 ggcagctctg gtggggtcac cagattccga tctggtactg cgatgcgtgc ggcgatacct
     2401 tcgcgagtgt cgaggagtcg gtcgacacgt gtccgtcgtg tggcgggccg gtccaccagg
     2461 acccagatgt gctcgacact tggttctcgt cggggctttg gccgttctcg accctcggct
     2521 ggccggagca gacggaggac ttgcggcggt tctaccccag cgacgtcctc gagaccggct
     2581 acgagatcat cttcacatgg gtcgcccgca tgatcttctt cggtatcgag atcatgggcg
     2641 aggtgccgtt ccacaccgtc tacctgcatg gcatcgtgcg ggacgacgag ggcgccaaga
     2701 tgagcaagac gaagggcaac gtgatcgacc cgctcgaggt ctcggaggag tacggagcgg
     2761 acgcgctgcg ttacacgctg gtgacgcaag cctcgccggg caacgacagc cgcctgagcc
     2821 tgcagaaggt cgaggcggga cgcaacttcg gcaataagct ctggaacatg acccggttcg
     2881 caatgcgcag cttcgatccg gacgtgatcg aggtggcgga ggacggcccg gctcggccga
     2941 tagggaacct gcagatcgtg gatcgctgga tcatcagccg gctcgatgcc gtgacggcag
     3001 gcgtttccgg gctgatggag cggcaccttt acggtgaggc ggggcggcag atcgccgact
     3061 tcctctggag tgaggttgcc gactggtaca tcgaggccgc caaggttcgc ctccgggagg
     3121 agggcgaggc ggcgcggatg gtggggcagg tgatcgtgta tgtgctggag cgcgccctgc
     3181 ggctgctgca cccatacatg ccgttcatca cggagacgct ctggcagcag ctgccgcatg
     3241 ccggcgagtc agtcatgatc gcgccatggc cggaagccgg cgagcgggac atgaccgccg
     3301 agcggcacgt cggcgcgatg atggagctgg tccgaggcat ccgcaacgcg cgcactgatg
     3361 ccggcgtcga gccggccaag tggatcgcgg cgatggtcta ccccggcgag cacctcgagg
     3421 cgttcgaaca gctccgcggc gagatcgcct tcctcgcgcg cgttcaggac gaccagctga
     3481 cctttgccga ggggaagccg gaggagtcgg gaaaggcgat caccgtggtg gcgggcgacg
     3541 tgatggcgtc gctgccgctt gaagggatgg tcgaccttgg cgtcgagcgt gagcggctcg
     3601 agaaagagct ggaggaggcc cgggcggaac agcagcgagc cgaaaagcaa ctctccaacg
     3661 agtcgttcgt gtctcgggct cccgagcatg tcgtccaggc tcaacgcgat cggctggagc
     3721 gggcgggcga gcaggtcgcc gtgatcgaac agcgtctttc ggcgctggcg gatgcctgag
     3781 ggaatgccgg tcgatcgtgc atggttctcg cgccaccccg tgaaggtggc gtgggacctg
     3841 atcggctgcg tgctgcgcat cgagcgggac ggcgtcgtgg tctccggtcg catcgtcgag
     3901 acggaggcct atgccggccc tgatgatccc gcctcacacg cgagccgatc accggtcggg
     3961 cgggaggtga tgatgggccg cccgggcact gtctatacct atctgtccta tggcattcac
     4021 acgatgatga acatcgttgc gcacgaggag aggcaggggg gcgccgtgct gctgcgcgcg
     4081 ctcgagccct tggagggcat cgaggtcatg ggggagcggc gcaatggcgt gtccgaaacc
     4141 cagttggcca aggggccggg cagcctcggt caggcaatgg atatccggct ctccgacctc
     4201 ggtgttgatc tcatcgacgc tcccgaatgg accctgctgc acggtgacgt acgccacccg
     4261 gttcacgccg gaccgcgcgt cggtatcagc aaggcggtag tggcgccgtg gcggttcttc
     4321 gagcgcccga acccgcacgt gtctcggcat cggaagggcg agcccgtcga gcggcacgag
     4381 ctaccgtcgt tgatcccgcc gtccgggacg ccgctcgagt gagtcgttta tgctgaaggg
     4441 tgatcgaagc gattctgcga ggcgcgccgt gaccatccga ctgctgaccg aagataccat
     4501 cgggaagatc gccgccggcg aggtcgtcga gcgaccggtc tccgtggtca aggagctgat
     4561 cgaaaacgct gtcgacgccg gtgcgaccag gatcgatgtc gacatcgccg ggggcggctc
     4621 cgagcgcatc cgcgtctcag acaacgggct cggcatgacg cggagcgatc tcgagctggc
     4681 aatccagcgc cacgcgacct ccaagctctc caccttcaac gacctcgaga cgctcagcac
     4741 gctcggattt cgcggtgagg cgctgccgag catcggcgcc gtctcgcatt tcatgattcg
     4801 cacggcagcg gcgggcgagg cgcgcggggc gcagctcgtc tccgcgttcg gtcgcaaggc
     4861 ggaggttgct gtcgtcgcgg cgcgacaggg aaccgtggta acggtcgagg atctcttcgg
     4921 gaacgtccca gcgcgccgga agttcctgcg ccagccgggc accgaggcga cctacatttc
     4981 ccgtctggtg agcgcctacg ccgccaactg gagcgacatc gcctggtcgc tcacgtccga
     5041 agcgcggcgg tccatctcga cgcaggggaa cggcgatgac atccaggcgg cgatcggcat
     5101 ctatggcacg gaactggtcg atgccgtgct gccgctctca tccgacgctg agggagcgag
     5161 ggtggagggc atcgaggtct cgggctggat cttggcacct cgtgtgtcac ggtcgcaccg
     5221 gcagaacctc ttcttcttcg tcaatggacg tctgatccag catcgctccc tcgtctacgc
     5281 attggaggag gcctaccaca gcctgctgat ggtgggtcgg cacccggtcg gcatggtccg
     5341 catcgacctt gacccgcgcg cggtcgacgt caacgtccat ccgacaaagg cagaggtccg
     5401 cttcgccgac gagcgcgccg tggcccgggc cgtgcagcgc gccgcgcacg cagcgttgac
     5461 gatgcagcag caggacgttc tgccggaggt cgcctacgag tgcccgcagg gtccaccgtc
     5521 ggtgcgacag acgtttttcg agcaccagca gcaggcgccg cagcccggcg gagctacctt
     5581 cgcccggcgt cgcgcagatg aggatgacga gccggaccag gcgccgtctc cgggactgca
     5641 gcaccgctcc ggagtaccgg cgctgagggt gctggggcag gtgggtggtg cctacatcat
     5701 tgccgagggc ccgggcggca tatttctgat cgaccagcac gcggcacacg agcgggtgat
     5761 gtacgagaag atcctcgggc aggtggagac gaaggatatc gaccgacagc ccttgctcga
     5821 cccgctggtg gtcgacctcg cgccgcacga gctgacggta ctggagcgat cgatggacga
     5881 gttgcgggag atcggcttcg acctggagat gttcggggag cagtcggtga tcgtgcggag
     5941 tgttcccgcg gtcatgcagc gcgtcgacat ccgtgagcgg atccatctga tcctcgagga
     6001 gttggccgag gggggctccg gcgactcgtg gttcgactcg gtggcgatca gcgcggcctg
     6061 ccacacctcg attcgggccg gccaggcact gtcgctggac gaaatgcggg agctggtcgc
     6121 gcagctcgag cggacccgcc agccgagggc gtgcgggcac ggtcgcccga caatgctgca
     6181 catgtcccag gcagacctgg agaaacagtt ctcccgccga taggacgtgt ctgcatcgag
     6241 cggacacaat gcccctacga gtttgccgtc tcgtcgcacc gcccggcgag cggcgtcgcg
     6301 tcgcccgcat agaggccgcg gcggccgttg agccagacct ggaccacatc ccgaaggttc
     6361 tggagtgtgt cgcgaagcgg actgaccttg ctgctggtgc cataggacca cgtgacaggg
     6421 atcacggcga tagcgaggcc gagctgccgc gcgatgaaca gcagctcgac atcgaaggcg
     6481 gtcacgcgag ggtgggacac ttcctcagag ttccggtaga gctgcacccg tgagagaatc
     6541 tccgtcgcgg cctcgctggt catgaacttg aatccgcact gcgtgtcatg gatgccaggg
     6601 aggatcagca ggcggaccag ccggttgaag agtctcccca tgatatgccg gtactccggc
     6661 tcgccgatgc ggcgcgaccc ctcgccctcg cgtgagccga tggccacgtc ggcaccgccc
     6721 tggatcagct cgactgcctc gacgagcgtc ggcaggggag tggcgaggtc agcgtccatg
     6781 aacccgacga tcggttgtgt ggcgacccgg agcccggcca gcaccgcgcg agccttgccg
     6841 ccgtgcggca gcgtcagcag gtggaaatgt gggtacgacc cggcgtggcg cgcggcaatg
     6901 cctcgcgtgt cgtcggtgct gccgtcatcg gcgatgatga cctcggccct aaatggagct
     6961 gcgcgcagaa actcggcgag ctgctccagc gtcgagccga tgcgctccga ttcgttgtat
     7021 acagggatca cgatcgatac gccgggcaag cttggagtgc cgctcgatgg ggtggttgta
     7081 gactccaacg gcgcgttgct cctgccttgc tcgcgatcga atcgaggaga agtctagcac
     7141 gcccggtcgc cgcttccggg cgcggagatc cgcatgaaac caattctcta cctctatcgc
     7201 tcctgcacct catgtcgcaa cgctgcttcg atcctcgacg cccacggcgt ggactatgac
     7261 gtgcgggagt acttcaagga accgttcacc agagaggagc tggagtcggt gctcgagcgg
     7321 gcagggcaga agccctccga cctggtggcg gcgcgcagcg ttccctaccg tgcccaaggg
     7381 ttggcggagc gagggctctc cgaggacgag ctgctggagc ggatgcttgc tgagccgcgc
     7441 ctggtgcggc ggccgatcct ggtcaccgaa gacgaggtgg cgatcggatt cgaccgcgac
     7501 cgatatgagc agaaggcaaa gacgctggcg agccgtgttt cggctgacgc ctaactcgca
     7561 accctgctga aaggaccctc gatgagcctg gcgtttggtg cccatgtgtc cgtttcgggc
     7621 ggtgtggaca aggcgttcgc gcgcgccgaa gagttccaga tggacgggat tcagatcttc
     7681 tcgaagaacg agcggcagtg gcgcgccaag ccgctcgccg atgatgttat cgagcgttgg
     7741 ttcgacgagc gcgaacggac cgggatcagc aagatcgtcg cgcacgcctc ctatctcatc
     7801 aatctggcga cgccgaggga ggacctgcga gagaagtcac tggcggcctt cctcgaggag
     7861 ctggaccggt gtgagatgct ggacatcccg tcgctggtaa ttcacccggg cgcgcacacc
     7921 ggtagcggag aggatgtcgg cgtcaaggcc gtcgccgacg cgctcaacca ggtgcacgag
     7981 ctccggcccg accacaaggt ccgaacattg ctcgagacga cggcggggca ggggacaacc
     8041 ctaggccgga cctttgagga gctggccgcg atcatcgacc aggtcgaggc gaaggagcgc
     8101 gtgatggtct gcctcgatac ttgccacgtc ttcgctgccg gctacgacat ccgcaggccg
     8161 gagagctatg ccgcagtgat gctgcacttc gcccatacca ttggcatcga tcggctccag
     8221 gcgatccacc tcaacgactc gcagaacggg cccggtacca acaaggaccg gcacgagcac
     8281 atcggtcagg gtgagatcgg cctcgagggg ttccgcaata tcgtgaacga cccgcgcctg
     8341 atcggcgtgc cggggctgct ggagacggag aagggcgaaa acaacgtcga agacggccgc
     8401 aatctggcga ggctgcgcga cctcgcggag cggccagtcg gaacgacggc cgagtagtcg
     8461 gtggtgcagc aggaacctct gcagcccgcc tgggcggctc ttgggttcct ggcaaccatt
     8521 ggcacgccgg aacacacaac gctgccagac gcgcccatgg ttgatgtcct ccagagacag
     8581 cgccttccat caagaacccc cagtgcccaa cagcaccggg ggtttccact cttgcaggtc
     8641 gcgtgacacg cgatcgtcag tagagttccg aggcgacatc ccggaagcgg tcgtacttcg
     8701 ttggggcatt ggtgttctga tggtccagtc cgatgccgga ctcccaccag gtcggcatct
     8761 gcgcgccgat ctgtgccaac tccgtctttg tgtagatcag gccgtcgcgc tccagcaccg
     8821 agagctcata ctcgtggctc atcgtgatcg catcgaccgg gcaaacctgg gcgcagcgtc
     8881 cgcagaacat gcaccggccc gccttcagca cgaactcgcc cagctcccgg tcgccctcct
     8941 cggacgcgac gatgttcatt tcgaggcagg tggtcgggca gatgcgcgcg cagaggccgc
     9001 aggcgacgca gagcggctcg cccgtggccg ggtccgtgcg cagcgatggc aatccacgaa
     9061 agcgcggctc catcgggcgc ttctcttccg gatactcggt tgtcactttc ggggaggcga
     9121 ggcgcttgag cgtaaccgca aaccccttga cttcatcaaa catcggcaga ttccttctct
     9181 ggtggtctgt gtccacgacg ttgctggccc atcaccgtcg gggtcaggcc cgtcgtgcga
     9241 tcgaggacat gcaccccgtc cccgccgaaa ccggccgttg gagtgacgac accctcgcgc
     9301 tcgaggaacg cgtaggacat tccgttgtaa ccgggcacga cggtcgcgat ctctcgcatg
     9361 acatgtgacg ggtggccgta ttcgagccga tagccgaagc gggccgcgat ctccgtcagg
     9421 tattccagcg acgactgtgc ctcgccgagc gcggggactg cggcgcggac ccgctgaacc
     9481 acccggtcaa agctggtgaa ggtgccgtcc ttctcgaggt tggctgaaac cggcaacacc
     9541 acgtcggcga tcgcggcgag cggcgagtcg tagatgtcgg cgacgacgag atactcgagc
     9601 ttctgcagcg cctcaaagag gcgcgcattc gggaagaagg tacgcgtgcg cgcgccgccc
     9661 tcgacccaca tcgccttcac cctgccggac tcgatggctt cggccaatcc ctccatgccg
     9721 atgcccggct cgctcggaag ttcctgctgc tgcacgtagc cattcgaggt cgcgactcga
     9781 ccagcccagc ggctgttcca cgtgcgctcc aggctgacgc gggcgctctc gtcgctcact
     9841 ggaacgccgc ctggcagcat gtcgggcacc attcccatgt cggtcgtgcc ctggtagttt
     9901 gccggcccac gcggcgtcgc gataccggcg cccgccttgc cgaggttgcc cgtgagaatc
     9961 gacaagttgg tgcaggcgtc gacgatgctg ccggcatccg gatagtcctg catcccctcg
    10021 attccccagt cgtaggcaat ggactgaatg atgtcgtgcg caaccgtctg gataatcgtg
    10081 cccgggagtt cgcctggctt ggcggcccgg tccgactctt cgaggaagcc ggtcacgaat
    10141 atgcgcgctg cctggacgat atcgtcactt gccacgccgg tctcacccgc gaccgcgttg
    10201 agatcgatac cctcgagcct cttgacgagc gccgtcacgt cagcatcacc cggggactcg
    10261 aggttgagcg caacgatcgc ggcggcgatc gcgtcgacca ccttgtgtgt cgtaccgggc
    10321 ttcggctgca gccagagggc tgcgcggtca cacagtagat agtgctcggt ggagatgacg
    10381 acgatgcgcg cctcgcggta gaggcgcgag tggttgaccc agtaggagaa gaccggagcc
    10441 gccttgaaga ccgacgggcc gatgaccatg acattcttca cgtgagtgaa gaattcctgc
    10501 tggttgttcg tgttcgacac cgacagcccg agcgcatcgc ggaccgatcg ctccacgtcg
    10561 agctgactcg gtgacatcag gcggtcgaca ttgttggact gcatgaccgc acgggagaac
    10621 tgttgaagca cgtatgcctc ttcattcgtg ttctcggcag aggccagcgc agcgaacgag
    10681 gagccctggt agtgggccaa gttctcggca atctcgtcaa cggcctcgtc gatgtcgatt
    10741 tcgtaggagc gcccgtcctc gccacggacc cgagcatggt acaggcgatc tggcccctga
    10801 acgacctcgt gtccccaccg gccgtactga caggtgtagc cgtggttgac gccgcgctcc
    10861 cagaggtgag tggtgcgccg gacgattccc ttgtgcgact cgatgttgag cgtacaaccc
    10921 acatcgcaga agccgcagat cgtctcggtc gtgtccagct cccaggggtg gtgaccgaag
    10981 tggcggtcag tcagcgcgcc gaccgggcag acggcaatgc acatgccgca gttggtgcag
    11041 gtgttttcct cgagggagcg gccgaaggag gtggtgatgg tcgcttccag ggagcgattg
    11101 ctctgctcga tcgcgatgac gccgatcatc tcgtcgcaga cgcgcgtgca gcggttgcac
    11161 atgatacagc gatcccactt gtagtcgacg gtgtcgctga aatgctcgta cggctgcgcg
    11221 agcttcgggc ggcggtacgg gttggcgttg atgttgtgga ggtaggtgtt gtcctgcaga
    11281 tagcactcgc cggacttgtc acaggtcggg cagtcgagag cgtggtcgat gaggtacatc
    11341 tgcaggatgt actggcgcgc ccgcatggag tcgggggaat gagtgagcac ctcggcgccg
    11401 tcctggaaga cggctgcgca ggattccacg agcccgccgc gcttgccgag cacctcaaca
    11461 gtgcagatgc ggcacgaacc ccacgggcgg aggagaggtt gatagcacag gttgggaacc
    11521 tcgatgccgg cgatacggga tgcttcgagg atcgtcgtgc cctgcggtac cgtcacctcc
    11581 tgaccgttga tcaatccggt caccatctgc tgctcggttg ctgccaccat agtgcctgtg
    11641 atgcctccgt ttgccggcaa tcagcttccg gtcgtggggt ctggcggtgg gcgtcttcga
    11701 tgctcaggca ggcactcgct ccggtttcgg gcagtgccgc gacgccactg tttcctgaca
    11761 cagtccgacc tccgcggctg ccgtccgcag gagttcagtc gtctcgatgg tagcacaata
    11821 gcccgaaatt tccggggtgt tgcgcctgtc ttcggtagcg cgttgccaaa caggctatgc
    11881 ttcccgcatc atcccccggc gccctgcgag cggggtaaag gacgatgcct cgcggcatca
    11941 gatgggtcgt tcgcgcgacc cgccgaggag aacagttgtg gctgcacagg caatcggccg
    12001 aacctaccgc acggtcatcg gcctggaagt ccacgcccag gtcctgaccg gttccaagat
    12061 gtactgtccc tgctccgcca gctacgccga cgccgcgccc aacacgcacg tctgcccggt
    12121 ttgtctgggg ctgcccggcg cgctgcccgt gatcaaccgg gcggcggtgg agatggtgat
    12181 tcacacgggg ctcgcgctca actgcgagat cgccagcgac tccaagatgg accggaagaa
    12241 ctatgtgtat ccggacctcc ccaagggcta ccagatctcc cagtacgacc tgccgctgtg
    12301 tctcgacggt gcgctcacgt tcgaggtcga tggcgagctt cgtacctccg ggatcactcg
    12361 cgtgcatatc gaggaggaca ccggccggct cgtgcacgac cagtcggacg aggacgacag
    12421 cctcgtggac ctcaatcgat cgggggtgcc gctgatggag atcgtcggcg aaccggatct
    12481 gcagtcaccg gaggaggcgc gggagtacct catggcgctc cggcaaatcc tgcggtatgt
    12541 cggtgcatca acaggaaata tggaagaggg cgcgttccgc tgcgatgcca acatctcgac
    12601 ccgcagcgcc gacggcgagt tcaccggccc caaggtcgaa atcaagaaca tgaactcctt
    12661 ccgcgccgtt gagcgggcgc ttcgcttcga ggagcagcgg cagcgggagg cggcggagcg
    12721 tggtggcgag ctggtgcagg agacgcgagg ttgggtcgac gcacagggga tcactgtctc
    12781 gcagcgtacc aaggagcagg cgcacgacta ccggtacttt ccggagccgg atctcccgcc
    12841 cctccggatg aagaaaaccg atatcgaccg tatccgcgag tcgctgccgg agctccctgc
    12901 ccagcgacgc cgccgcctga tggacgagtt cgggcttccg cctggcgacg cggtgacgct
    12961 gaccatggag cggccgatcg ccgacctgta cgaagacgtc gtggcggcca ccgacgcgcg
    13021 tttccatcgc gcggtcgcca actggatcgt caacgacatt atggggctcg cgcgcgcgcg
    13081 cggcatggca caggaccagc ttccgttgtc cgccgcccaa atccgtgacc tcgtgcgggg
    13141 cgtggaaaat ggcaagctga ctgcacgggc ggcaaaggac ctgttgccac agatcgaaga
    13201 tggcgagatg ccgatggatg ccgctgagcg gttcaatctg ctctcgctcg atgatgcgga
    13261 tgttgtcacg caggcggccg ccgaggcgat cgaggcgaac cctgctgtgg tgcaggacta
    13321 tcttggtggc aagaaggctg ctgtgggtcg gttgatcggc gagaccatga agcggaccgg
    13381 cggtcgagcc aagcccgacg tggtgcgtga gacgctcgtc aagctgttga gccaggtgga
    13441 gggcgagtga ggatcttgac tgtgatggcg cagtgacgca ggagcgctga agggagaacg
    13501 caggatatgg tgacagcgac aggcatcagg gaagcggcaa cggcgccgga gcccacagaa
    13561 gatgaggata ttttcgatgt cgcggtacgg cagttccaca tcgctgcgga cgtggtcaac
    13621 ctcgatgacg gcatccgtgc cgtcttgagc gactgtgagc gagagctgac cgtgcacttt
    13681 ccggtggaga tggacgacgg ctcggtccag accttcaccg gctatcgggt ccagcacaac
    13741 agtggtcccg gtccgacaaa gggcggcatc cgttatcacc aggccgtcac ccgctccgag
    13801 gtcaaggccc tcgcgatgtg gatgacgtgg aagtgtgcgg tggtcggcct gccttttggc
    13861 ggcgcgaagg gtggcgtgcg ggtcaacccg aaaatgctct cggccaacga gatacagaac
    13921 ctgactcggc ggtacaccac agagatccag ccactcatcg gcccgacccg ggacattccc
    13981 gctccagacg tgaacaccaa tgcgcaggtc atggcgtggc tgatggacac ctactcgatg
    14041 aacatcggtt actccgagcg cggtgtgacc accggaaagc cgattgtgct gggcggatcg
    14101 gcagggcgcg aagaggcgac cggccgcggt accgtgttcg cggtcgagga tggcgcccgt
    14161 catgtcggtc tcgatctgtc gtcggcgagg gtggcgattc agggctacgg caacgtcggt
    14221 gctgtcgccg gccgactcat ggtacagcga aacaagtcca cagttatcgc cgtaagcgat
    14281 tcctcaggcg gcatctacaa tcccaacgga ctcgatctgg atgaggtcga tcgtgcgaaa
    14341 cggcgcaccg gtacggtgca ggggtacgcg gatgccgata aggtcacgaa ttccgaactg
    14401 ctcgagctgc cgtgcgacgt gctgattccc gccgcgctcg aggggcagat caccgggcgc
    14461 aacgctgacc gaatcaaggc cggcatcatt gcggaggcgg cgaacggccc gaccctgccg
    14521 gctgccgacg atatcctgta cgagcgtggc gtgctggtga tcccggatat ctacgccaat
    14581 gctggcggcg tgaccgtgtc gtactttgag tgggttcagg cgctgcaggc gttcccgtgg
    14641 accctcgatc aggtgaatga gcggctccgg cagatcatgc aggactcctt cacgcacgtc
    14701 ctcgcgaagt cgacggagta cggcgtacac atgcgcacgg cggcgatgat ccgcgcgatt
    14761 gagcgcgtct ccgagttcac tcgccttcga ggcatttatc catagggaag acccgttcgc
    14821 gacggcgagc aggcgtcccg atcgaagcgg agcatggcga tagcgctcgt gagctgtcgg
    14881 acagcggacg cagaaccgcc cgggcagtga tgaggtccgg gcggttctct tgtggcacga
    14941 gggcgacgct gacccttagc tggcctgcgt gagcgtccgc tggagctggg cttcctcgtg
    15001 gctgtgcgtg acgatgatca gctcatcgcc cggcaggatc tcggtgctct gcgt
//
LOCUS       DASYWL010000042         2971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_114415, whole genome shotgun sequence.
ACCESSION   DASYWL010000042 DASYWL010000000
VERSION     DASYWL010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114415"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="VGR22_01515"
     CDS             complement(<1..748)
                     /locus_tag="VGR22_01515"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEV2127276.1"
                     /translation="MPMHRSDDAPVLEVGEPRLLVDEPCETGEGPLWHEDEQALYWLD
                     IPTGRLFRYDPGSQVNEVVFRHDGEIGGLTIQQDGSLLLFCSRGQVLHWNDGKTTTVI
                     DEIPAERENRFNDVIADAEGRVYCGTLAYDGGPARLYRLDPDGSLTRLFDDIGLSNGM
                     GFTADGCTMYHTDTNFRVIYRLDYNRATGGISNRTPLLRTPEGNGAPDGMALDAEGTI
                     WSARYGGGRIFRYSPKGETIGIVPMPVTSVT"
     gene            complement(974..1906)
                     /locus_tag="VGR22_01520"
     CDS             complement(974..1906)
                     /locus_tag="VGR22_01520"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV2127277.1"
                     /translation="MADRAVYRGVFPIAPTTFNADESIDEASQRRAIDFMVDAGVDGI
                     CILANYSEQFSLTDAERDRLQAVCLEQVDGRVPVIVTTSHFSSRIAAERSRTAQEAGA
                     AMVMLMPPYHGATLRVEPDGLRRFFSTVADAIDIPIMIQDAPMSGTVLPAGLLADLAK
                     KIPQVQYLKVESPKAAEKLKAVIELAGDACPGPFDGEEGITLIPDLEAGATGTMPSAM
                     IPEVHGEVLRHWREGHREDARDAWEAWLPMIHYENRQCGLLATKTLMREGGVIASDMV
                     RAPLSPLSPMIREGLLAHARRRDPLVLRWARDHA"
     gene            complement(1926..2342)
                     /locus_tag="VGR22_01525"
     CDS             complement(1926..2342)
                     /locus_tag="VGR22_01525"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV2127278.1"
                     /translation="MVEGQKIYGRADIPAVIASLPPDPDSEARHRANTLIKTNTLRVV
                     VVTMLEGGELQEHTAPGPITIHTIEGGMEVDVEGETHPLDAGELISLAPGVRHAVRCT
                     RDGAFLLTIGVFLREPDPGGRHGGIEEHGRFLDQIP"
BASE COUNT          479 a          932 c          991 g          569 t
ORIGIN      
        1 tggtgacgct cgtcacgggc atcggcacga taccgatcgt ctcgcccttc ggagagtagc
       61 ggaagatcct acccccacca taccgtgccg accagatggt gccctcggca tcgagggcca
      121 tgccgtccgg cgcgccgttg ccctccggtg tgcgcagcag cggcgtgcga ttgctgatgc
      181 cgccggttgc acggttgtaa tcgagccggt agatcacgcg gaagttggtg tcggtgtggt
      241 acatcgtgca cccgtcggcg gtgaacccca tgccgttcga gagcccgatg tcatcgaaga
      301 gccgcgtcag cgagccgtcg gggtcgagcc ggtagaggcg ggcagggcca ccgtcgtagg
      361 cgagggtgcc gcagtagacg cgcccctcgg cgtcggcgat gacgtcgttg aagcggttct
      421 cgcgctcggc gggaatctcg tcgatcaccg tggtggtctt cccatcgttc cagtggagga
      481 cctgcccccg agagcagaag agcaggagcg agccgtcctg ctggatggtg aggccaccga
      541 tctctccatc gtgccggaag accacctcgt tgacttgaga gccgggatcg tagcggaaaa
      601 ggcgtccggt ggggatgtcg agccagtaga gcgcctgctc atcctcgtgc cagagcgggc
      661 cctcgcctgt ttcgcatggc tcgtccacga gcagccgtgg ctcacccacc tcgaggaccg
      721 gcgcgtcgtc ggatcggtgc attggcatcg gtgactcctt gtctcagggc tgcagccaac
      781 cgcgtcattc gagcccattc gactcgggct tgtggctctc gctcagaaca gactccgcgg
      841 ctttgccgcg gagtcgagaa atctcctggg tgatctgctc gagagtcgag ccctgacgcc
      901 atcgtcgtga gagatttctc cacttcgcat cctgtgggtg ctccggtcga aatgacatgc
      961 tgggcgacgc cagttatgca tggtcccgtg cccagcggag gacgaggggg tcgcggcggc
     1021 gggcgtgagc gagcagcccc tcccggatca ttggcgagag cgggctgagc ggtgcacgga
     1081 ccatgtcgct ggcgatgaca ccgccttccc gcatcagcgt cttcgtggcg agcaggccgc
     1141 actgccggtt ctcgtagtgg atcatcggca gccacgcctc ccaggcgtcc cgtgcgtcct
     1201 cccggtgccc ctcccgccag tggcgaagca cctcgccgtg aacctccggg atcatggcac
     1261 tcggcatcgt gccggtcgca cccgcctcga gatcggggat gagcgtgatg ccctcctcgc
     1321 cgtcgaaggg tccggggcag gcgtcgccgg ccagctcgat cacggctttt agcttctcgg
     1381 cggctttcgg cgattccacc ttcaggtact gcacctgcgg gattttcttc gcgagatcgg
     1441 cgaggaggcc ggcgggcagc accgtgccgc tcatgggagc gtcctggatc atgatcggga
     1501 tgtcgatcgc gtctgccacc gtggaaaaga agcggcggag accgtccggt tccacgcgca
     1561 gggtcgcgcc gtggtagggc ggcatcagca tgaccatcgc cgcgccagcc tcctgggcgg
     1621 tgcggctgcg ctcggcggcg atgcgcgaac tgaagtggct cgtggtaacg atcaccggca
     1681 cccggccatc gacctgctcg agacagaccg cctgcagccg gtcgcgctcg gcgtcggtca
     1741 ggctgaactg ctcggaatag tttgcgagga tgcagatacc gtcgacaccg gcatcgacca
     1801 tgaagtcgat cgcgcggcgc tgactcgcct cgtcgatcga ctcgtcggca ttgaaggtgg
     1861 ttggcgcgat cggaaagacc ccgcgataga cggctcgatc agccatgcct acagctttct
     1921 actggtcaag gaatctggtc aaggaatcgg ccgtgctcct cgatgcctcc gtggcggcca
     1981 ccgggatcgg gctcgcgcag gaatacgccg atcgtgagca ggaaggcccc gtctctggtg
     2041 cagcggacgg catggcggac gcccggcgcg agcgagatca gctcgccggc gtcgagcggg
     2101 tgcgtctcgc cctcaacgtc gacttccatg ccgccctcga tcgtatggat ggtgatcggt
     2161 cccggtgcgg tgtgctcctg cagctcgcca ccctcgagca ttgtcacaac caccacccgc
     2221 agggtattgg tcttgatcag cgtgttggcc cgatggcggg cttcgctgtc cggatccggg
     2281 ggaagcgacg cgatcactgc ggggatgtcc gccctcccat aaatcttctg cccttcgacc
     2341 atggcatgcc ctcctgattc ggctggcgtc atcttgcccg atcgtcgacg tggtgtatag
     2401 cccgcacggg cgtggctcgc ggtctgaatc gcaatcccgg taccagtggc agcaatcgca
     2461 gcaggcgcat tcgatgcgtt cgaaagatta catgaggtgc ggggatgcgc gttcacggag
     2521 ttaccgagcg gagcctcgtg tttgttcgcg cgaggatcct ggtcgcgttg gacggtttga
     2581 gaaccattgc cacccagggg gcgacggctc gatcactgat cggcaggtcg acctcaatac
     2641 cagcttcatt gtccaacagg atttgtcgac ctcgtggtgt gccgaattgc acaagaattc
     2701 tcgctcgatt tgtgcggctt gtccctcgaa tccgctcgag cagcgcttca cactgaatcc
     2761 attgggcgac agagcagtcg cactgcccga tgttgggcag agtgcacagt tgacgcggtg
     2821 ttcggagggt gacaccgcga gccaagcatg gaggggagtt tccctatgga cactggtaac
     2881 acggctttcg tcctggtcgc ggccgcgctg gtcatgctca tgaccccggc gctggggttc
     2941 ttctatgggg gtctggtcaa ccgcaagaac g
//
LOCUS       DASYWL010000043         2622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_208312, whole genome shotgun sequence.
ACCESSION   DASYWL010000043 DASYWL010000000
VERSION     DASYWL010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208312"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VGR22_01530"
     CDS             <1..358
                     /locus_tag="VGR22_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127279.1"
                     /translation="AMPTEYPDATPQEPIRFRVSAIERFALPNLVKQHHMVLRVEYLD
                     PEDPLRGASFDLSPERYRPYREAIDEALASGATYWVDWDYTKTPNFPLRLRESAAERL
                     LRAIGDRQIVSTNGEE"
     gene            complement(1168..2010)
                     /locus_tag="VGR22_01535"
     CDS             complement(1168..2010)
                     /locus_tag="VGR22_01535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEV2127280.1"
                     /translation="MTTDERKVGRRGAIALRDAEGFIFDMDGVLYRGKRALAGVVEIF
                     DALDARGIPYLLATNNSMATPAMYVERLKGYGVTVDASQIQTSATATRDVLGHEFPEG
                     GTLFVVGMPALDEQLQDGTDFHITSDPTDDIVAVVVGLDQQFTYDKLAAATTAIRGGA
                     RFFATNDDATLPVEDGILPGAGSIIAAIATASGRQPTVVGKPQTLMMTDAVERLGSQA
                     HCTVMIGDRLDTDILAAERAGLTTALVLTGVTTREELAAGESVPDYVFSDLPALTREL
                     VGTD"
     gene            complement(2077..>2622)
                     /locus_tag="VGR22_01540"
     CDS             complement(2077..>2622)
                     /locus_tag="VGR22_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEV2127281.1"
                     /translation="QMDNSGKKVAMVLAPNFEDVEATDPKQALEQAGAQVTIIGIEKG
                     EIEGKKGAKLEATETFETAKPEDYDMLVIPGGGSPENLRIHDAAVAFTRQFVESGKPV
                     GSICHGPQLLISAEVIGGRTVTAVAKIRDDIKNAKANYVDEELVVDGNLITSRVPKDL
                     PAFNQALLTALAERVPAGQAD"
BASE COUNT          443 a          848 c          808 g          523 t
ORIGIN      
        1 agcgatgccg accgagtacc ccgacgccac cccgcaggag cccatccgct tccgcgtctc
       61 cgccatcgag cggttcgcgc tcccgaacct agtaaagcaa caccacatgg tcctgcgcgt
      121 ggagtacctc gacccggagg atccattgcg cggggcgtcc ttcgacctat cgcccgagcg
      181 gtaccgaccc taccgggaag ccatcgacga ggcactcgcc tctggcgcga cgtactgggt
      241 cgactgggac tacaccaaga cacccaactt tccgctccga ttgcgagagt ccgccgccga
      301 gcgcctgcta cgggccatcg gcgaccggca gattgtctcg acaaatgggg aggaatagga
      361 gtgccggaca agcggtgaca gccaggcacg atccgaagat gagcgcgctg tgtggtatcg
      421 cgatgcacag cggtcgctcc cgtgcctgct gtcccggcat gcctgatcgt gcccatctgg
      481 gggcggttcg ctgtcctgtt gtccgagcgt cccgcatgtg catccgatat atttcttgat
      541 ccgccgggaa caactcggac tgtcaccgtc atgggtacca cgcccacggc gacctttgaa
      601 cttcttggct cggaaccggg gcgagcagca ctcaccgacc taccttccgc tgatcaatgc
      661 cgggcctctg ctgccgcctt tctgctgccg atatagctgc cgtcgccggt gtcgcgttgc
      721 atggccgtgg aatcatccac cgacgtcagt cgaggacggc ctgcgattcg agatcaggcg
      781 cgtctggccg gcggcgagtg ccggcggaca gagcgaatcg tctcgcgaga tacgccgtac
      841 tcagcagcga gagatcacag gctctggtat ttcaatcgag ccgtgatttt agcctcgtct
      901 tgagatggag agcttgcgag ggcgctgaga atatcgctgt ccttccacgc atgggaattc
      961 aactcttgct tgaggatagc ggtggagtgc gacacgacgg gctttgaaga gcgctaccat
     1021 acggttccgg tccccgtcgc ttccgccaat atgcccgatg tcacgtggca tcgggtcttt
     1081 tgcgttgcgt tttcgagcat atctgccgag gcactgtgcc ccgctgatcg aggtgtgatc
     1141 cgagagcgcc cgacgcgctg ccacttgtca atcggtgccg acgagctcac gggtgagggc
     1201 cgggaggtcc gagaagacgt agtcgggaac cgactcgccg gcggcgagct cctcgcgggt
     1261 ggtcacaccg gtgaggacca gcgcggtggt aaggcctgcc cgctcggcgg cgaggatatc
     1321 tgtgtctagg cggtcaccga tcatcacggt gcagtgcgct tgcgagccga gccgctcgac
     1381 ggcatctgtc atcatcagcg tttgcggctt gccgacgacg gtcggctgtc tcccgctggc
     1441 cgtagctatc gccgcgatga tcgacccagc tcccggcagg atgccgtcct cgaccggtag
     1501 cgtcgcgtca tcgttggtcg cgaagaatcg ggcgccaccc cgaatcgctg tcgttgcagc
     1561 cgcgagcttg tcataggtga attgctggtc gagccccaca acgacggcca caatatcgtc
     1621 ggtcggatcg ctcgtgatgt gaaagtccgt gccatcctgc agttgctcgt ccagcgccgg
     1681 catgccgacg acgaacaggg tcccgccctc ggggaactcg tgcccgagga catcgcgcgt
     1741 cgccgtagct gatgtctgta tttgggaggc gtccaccgtg acgccgtacc ccttcaggcg
     1801 ctcgacgtac atagccggcg tcgccatgga attgttggtc gcgaggagat agggaatgcc
     1861 gcgtgcatcg agagcatcga aaatctcgac gacccctgcc agagcccgct tgccgcggta
     1921 gagcacgccg tccatatcga aaatgaaccc ttcggcatct ctcagagcaa tggcgccgcg
     1981 cctgccgact ttccgctcgt cggttgtcac gtggtggacc ttcccgcttg gaattaacag
     2041 cggtgtggag cagcttgccg ctccacaccc gagattctag tccgcctgtc cggccggcac
     2101 tcgctcggcg agtgcggtaa ggagcgcctg gttgaacgcc ggaaggtcct tcggcactcg
     2161 tgaggtgatg aggttgccgt caaccaccaa ctcctcgtcc acgtagttcg ccttcgcgtt
     2221 tttgatgtcg tcgcgaatct tggcgacagc ggtgacggtc cggccaccga tgacctctgc
     2281 cgagatcagc aactgcggtc cgtggcagat cgagccgaca ggcttgcccg actcgacgaa
     2341 ctgccttgtg aaggcgaccg ccgcatcgtg gatgcgcagg ttctcgggcg accccccacc
     2401 tgggatcacc agcatgtcgt aatcctcggg cttcgcggtt tcgaaggtct cggtcgcttc
     2461 gagcttcgcg cccttcttgc cctcgatctc gcccttctcg atcccgatga tggtgacctg
     2521 cgccccggcc tgctcaagcg cctgcttcgg gtcggtcgcc tcgacatcct cgaagttggg
     2581 tgcgagaacc atcgctacct tcttcccaga attgtccatc tg
//
LOCUS       DASYWL010000044         6105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_133122, whole genome shotgun sequence.
ACCESSION   DASYWL010000044 DASYWL010000000
VERSION     DASYWL010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6105
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133122"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VGR22_01545"
     CDS             <1..234
                     /locus_tag="VGR22_01545"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEV2127282.1"
                     /translation="GKTTEEIELYRVTDVGVKQHPLERITKMGDIYVASGDTSTPTKY
                     LHNVVDPERVKDLVREATRQERERRRVFVREDF"
     gene            379..1137
                     /locus_tag="VGR22_01550"
     CDS             379..1137
                     /locus_tag="VGR22_01550"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEV2127283.1"
                     /translation="MTLTIWNESTRGELNGALPVALVVLPTAAIEQHGPHLATGHDTF
                     MVAHIARQAAERAAGQADVVLAPVLAFGSSAHHLPFGGTLSLRTETYLHVVRDLIESM
                     LACGAKRILLLNGHGGNHELNQLAARDIAIELSPESGVVLSAASYWDIAREALAEDEV
                     AGGLTLPGHAGQFETAMMLAFDPLRVRQRVERQADPSATDVVPGVRVETPDRWESFDG
                     YTDFPHRATAEQGEVLLERVVGAVAEAMVALARR"
     gene            complement(1251..3401)
                     /locus_tag="VGR22_01555"
     CDS             complement(1251..3401)
                     /locus_tag="VGR22_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEV2127284.1"
                     /translation="MAARTIPLAHTVHTACPHDCPDTCAITTTTESGRAIAFTPVRDH
                     PVTRGWLCAKVRPYLDRVYHPDRLTTPLRRTGSKGSDQWEAITWDEALEEITDRWQAA
                     ISRSGAGAILPYSYSGTLGLVQMGVASARLWNRMGACGLDRTICDAAAGVAVPATVGA
                     KHAMAFEDFLQSELIVLWGHNPASTSPHFMPFLRGAQKNGATVVLIDPRRTLTAKSVD
                     WHMQPEPGTDAALALGVIHVLFQKHLHDEAWLEAHSTGWRDLRDRAAGYDPERVSEIT
                     GIPVEDIERLAGLWAKSSPAVVKIADGLQRNQHGGQVVRAAISIPAVTGQYGILGGGM
                     FYSQSGWITWDSEAVGHGSDSACPPIPRVVNMNRLGAALTGEDGGAPIESLFVFGANP
                     VTSTPNSPKVVDGLLRDDLFTVVHEQFMTDTATYADIVLPATSQLEQVDLHKPYGHHH
                     LQYNAQAIEPLGECRSNWDVMRALAERMGYDEPWLRQEPDAIIKEVLCATARANPLLE
                     GITLERLQREGTVPYSFGTVSDIPFEGGTFPTHDGRIQLRCDAMLEHNVDPLPDYTGP
                     ESPDEAREDGSLRLLSGAAHHFVNSSMANQPNLERKEGRPTLEISPADAERLGVSDGQ
                     IVRVYNERGSALLHASVTDGVRPGVVVSTKGRWGQRSLDGRNVNQLTTDELSDLGGGS
                     SFHGTRVFIAQATTDDLQLFEVASRNRNAVAVQQ"
     gene            complement(3406..4596)
                     /locus_tag="VGR22_01560"
     CDS             complement(3406..4596)
                     /locus_tag="VGR22_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEV2127285.1"
                     /translation="MASSTIAAPAATPDIAPETLRKHFQLDTGITFLNHGSFGATPKP
                     VFDVYQAWQRELEREPVAFFARRQEALLDAARGRIGTWLNADPDTLSFVVNATSGLNV
                     IARSLSLGPGDEILTTDLEYGALDLTWEHLCAKAGARYFHQPISLPVTSKEEVVETIW
                     AGVSDRTRAIFLSHITSGTTLILPVQEICARAREVGILTIIDGAHVPGQLDLDMEAIG
                     ADAYSGNFHKWLCTPKGSAFLYVRPEQHGWIESLTISWGWMGEHTFVTRNQKQATRDV
                     SAFLAVGRAIDFQREHNWDAVRDRCFEMLSTLRQELHAKWGTTAISPDTPDWYRQLAT
                     ITLPDSAPDDLQDRLFHNHAVEVPITRHGDRRFIRVSVQGYTTERDLDALRTALNAEI
                     TTGA"
     gene            complement(4753..>6105)
                     /locus_tag="VGR22_01565"
     CDS             complement(4753..>6105)
                     /locus_tag="VGR22_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV2127286.1"
                     /translation="PVTAQDYVRTFQYAADPEHAWDFTWFWNGNIVNFEEATAGEVPV
                     EEVGVREGANEYEVVFETIEPAPYLAAKLLYSTPLSAAALDEYGPLYNTNPETHVSSG
                     PFKLEEWLRDQQIVYTRNEAYTGDMVVALQEIIVKLAAPPSHFTLYEAGQVDYMEGPA
                     PAELQIMEQDPETTTQIYQGVGDFRCFYFFFDVERAPFDDLQVRQAFSHVLDREALDQ
                     QVWGRQANPAPSFLAPGYPASNTEELAGIQNFDADFGRQLLAEAGYPDGEGFPELTMN
                     IVVGQNPIYDATAEAYASMLSEHLNIDVQIQSLDPQAFYAAMNADPDEILFGFISYGM
                     DYFDPSNMLGVWHSTGRHSWSNEEYDALVEEGTTSLASQEERIAIFQEAERILVEDVP
                     AVFTYFQTPIQLIKPYVTGPALEPDNNGIQAIHWPAWATTGHTLEELYIRADAPTGRE
                     "
BASE COUNT         1001 a         1855 c         2129 g         1120 t
ORIGIN      
        1 ggcaagacga ccgaggagat cgagctctac cgcgtaaccg acgtcggggt gaagcagcac
       61 ccgctggagc gaatcacgaa gatgggcgat atctacgtgg catcgggtga taccagcacg
      121 ccgacaaagt acctgcacaa cgtcgtcgat cccgagcggg tcaaggatct cgttcgggag
      181 gctacccgcc aggagcgcga gcgccgccgc gttttcgtcc gtgaggattt ctagcaagcg
      241 acaggagcgg tagcggcgcg ccctgaaaag gcacccggcc actcgttccg ggaatgacag
      301 gcggcgacgc cagcgcgtgc gctgattcga ggccacacgc tgccgggatc gaccctcacg
      361 gattcactgg agccgcatgt gacgctgacg atctggaacg aatccacgcg cggggaactc
      421 aacggggcgt tgccggtcgc gctcgtcgtg ctccccaccg ccgcgatcga gcaacacggg
      481 ccacacctcg caaccggtca tgacacgttc atggtggcgc acatcgcgcg gcaggcggcc
      541 gagcgggcgg cagggcaggc agatgtcgtg ctggcgccgg tccttgcctt cgggtcgtcc
      601 gctcaccact tgccgttcgg tggcacgctc tcgctgcgaa ccgagaccta cctccatgtg
      661 gtgcgcgacc tcatcgagtc gatgctcgcg tgtggcgcga agcgcatctt gcttctcaac
      721 ggccacggtg gcaaccacga actcaaccag ttggccgcac gcgatattgc gatcgagctg
      781 tcgcccgaga gcggtgtggt gctctcagca gcgtcctact gggacatcgc gcgggaggcg
      841 cttgccgagg acgaagtggc cggcgggctc accctgccgg gccacgccgg gcagttcgag
      901 accgcgatga tgctcgcgtt tgatcccttg cgagtgaggc agcgggtcga gcgccaagct
      961 gatccatcgg ccaccgatgt cgtccccggt gtgagggtcg agacacccga ccggtgggag
     1021 tcgttcgatg gatataccga cttcccacac cgggcgaccg cggagcaggg cgaggttctg
     1081 ctggagagag tagtcggcgc cgttgccgag gcgatggtgg cactggcgag gcggtagctg
     1141 gcggcctcta tccgacccct gtcatgtcga gccggcaagg cggtttgccg ccggagccac
     1201 gtcgagacat cgtgacgcca gtgtcggttg ctgggcgcga tttgcatggc ctactgctgc
     1261 actgcgaccg cgttccggtt cctcgatgcc acctcgaaga gctggagatc gtcggtggtg
     1321 gcctgggcaa tgaagacgcg ggtgccgtgg aaggagctgc cgccaccgag gtcggagagc
     1381 tcgtcggtgg tgagctggtt gacgttgcgg ccgtcgagcg agcgctggcc ccagcgacct
     1441 ttcgtggata cgacgactcc cggacgcacg ccgtcggtca cggatgcatg cagcagcgca
     1501 ctcccacgct cgttgtaaac gcgcacgatt tgtccatcgg agacgcccag ccgctcggcg
     1561 tcggccgggc tgatctccaa tgtcggacgg ccctccttgc gctctaggtt cggctggttg
     1621 gccatggatg agttgacgaa gtggtgtgcc gcgccggaga gcaggcggag ggagccgtct
     1681 tcccgggcct cgtcaggtga ttccgggccg gtgtagtcgg gcagcgggtc gacattgtgc
     1741 tccagcatcg cgtcgcaccg cagctgaatg cggccatcgt gggtcggaaa tgtgccccct
     1801 tcgaagggaa tatcgctgac cgtgccgaag ctgtacggaa cggtgccctc gcgttgcagc
     1861 cgctccagcg tgatgccctc cagcagcggg ttggctcgcg ccgttgcgca gagcacctcc
     1921 ttgatgatcg cgtccggttc ctggcgcagc cacggctcgt cgtaccccat tcgctcggcc
     1981 agcgcgcgca tcacgtccca attgcttcgg cactcgccta gcggctcgat cgcctgcgcg
     2041 ttgtactgca ggtggtggtg gccgtagggc ttgtggagat caacctgctc cagctgactg
     2101 gtggcgggga ggacgatgtc ggcgtacgtc gccgtgtcgg tcatgaactg ctcgtggacg
     2161 acggtgaaga gatcgtcccg caacagcccg tcgacgacct tcggactgtt cggggtcgat
     2221 gtgaccgggt tggcgccgaa gacgaagagt gattcgatcg gcgcaccgcc atcctcgccg
     2281 gtcagcgccg ccccgaggcg attcatgttg accacgcgcg ggatcggtgg acatgcgctg
     2341 tcgctaccat gccccactgc ctcgctgtcc caggtgatcc agccgctctg gctgtagaac
     2401 atgccgccgc cgagaatccc gtactgcccg gtgacggcgg ggatcgagat cgccgcgcgt
     2461 acaacctgcc cgccgtgctg gttgcgctga aggccatcgg cgatcttgac cacggccgga
     2521 gaggatttgg cccagagtcc tgcgagccgt tcgatgtcct cgacagggat gccggtgatt
     2581 tcgctgacgc gctctggatc gtaccctgcg gcgcggtcgc gcaggtctcg ccagccggtg
     2641 ctgtgggctt ccagccacgc ctcgtcgtgc agatgtttct ggaacaggac gtggatcaca
     2701 ccgagcgcga gcgcggcgtc cgttcccggc tccggctgca tgtgccagtc gactgacttc
     2761 gccgtgagcg tccggcgtgg gtcgatcagc accacggttg ctccattttt ctgtgcgccc
     2821 cggagaaatg gcatgaagtg tggactcgtg ctggccgggt tgtgccccca gagcacgatg
     2881 agctcgctct gcaggaaatc ctcgaacgcc atcgcgtgct tggcgccgac agtcgcgggc
     2941 acggcgacgc cggcggcggc gtcgcagatc gtccggtcga gtccacacgc gcccatccgg
     3001 ttccagaggc gagcgctggc gacacccatc tgcacgagcc cgagggtacc gctgtaggag
     3061 taggggagga tcgctccggc gccggaccgg gagatggccg cctgccagcg gtcggtgatt
     3121 tcctcgagcg cctcgtccca tgtgatcgct tcccactggt cgctgccctt cgacccggtg
     3181 cggcggagcg gcgtggtgag gcgatccgga tggtagaccc gatcaaggta ggggcggacc
     3241 ttggcgcaga gccagccgcg cgtgacgggg tggtcgcgaa ccggcgtgaa ggcaatcgcg
     3301 cggccgctct cggtggtggt ggtgatcgca caggtgtcgg ggcagtcgtg cgggcaggcg
     3361 gtgtgaaccg tgtgggcgag cgggatggtc cgtgctgcca tacgctcagg ctcctgtcgt
     3421 gatttcggca ttcagcgccg tgcgaagcgc atcgagatcc ctctcggtgg tgtatccctg
     3481 caccgagacg cggatgaacc gccggtcgcc gtgcctcgtg atcggaacct cgacagcgtg
     3541 gttgtggaag agccggtcct gcaggtcgtc tggtgccgag tcgggcaggg tgatggttgc
     3601 caactgccgg taccaatcag gggtgtctgg tgaaatggca gtcgtgcccc acttcgcgtg
     3661 cagttcctgc cggagcgtgc tcagcatctc gaagcagcga tcgcggacgg cgtcccagtt
     3721 gtgctcgcgc tggaagtcaa tggcgcgacc gacggcgagg aaagcggaga cgtcgcgggt
     3781 ggcctgtttc tgattgcggg tgacgaaggt gtgctcgccc atccaccccc agctgatcgt
     3841 cagcgattcg atccagccgt gctgttccgg tcggacgtag aggaacgccg accccttggg
     3901 cgtgcagagc cacttgtgga agttaccgga gtaggcgtct gcaccgatcg cctccatgtc
     3961 gagatcgagc tggccgggga cgtgggcgcc gtcgatgatc gtgaggatac cgacttcacg
     4021 tgcccgggcg caaatctctt ggacgggcag gatcagtgtc gtgcccgagg taatgtggct
     4081 caggaagatg gcacgggtgc ggtcggaaac gccggcccag atcgtctcga cgacctcctc
     4141 cttcgacgtg actggcagcg agatcggctg gtggaagtac ctcgcgccgg ccttcgcgca
     4201 gagatgttcc caggtgaggt cgagcgcgcc gtattcgagg tcggtggtca ggatctcatc
     4261 gcccggcccc aaggacagcg agcgcgcgat gacgttgagt ccggaggtcg cattgacgac
     4321 gaagctgagg gtgtcggggt cggcgttgag ccaggtgcca atgcgaccgc gcgcggcatc
     4381 gagcagcgcc tcctgccggc gagcaaagaa ggcgacaggc tcgcgctcga gttcccgctg
     4441 ccatgcctga taaacgtcga agacgggctt cggcgtcgcg ccaaaggagc cgtggttgag
     4501 gaaggtgatt cccgtgtcga gctggaagtg tttgcgcagc gtttccgggg cgatatcggg
     4561 cgtggcggcg ggagcggcga tcgtggacga ggccatggcg gtcctttcgg caggcgtagt
     4621 cgtgggcgtc gccccggtcg gtgcaactga cgtaccgatc atagcgccag cgtcccgctg
     4681 ctggcgacca cggaaacggc ccactgttgc tcaagagagc gacagcgggc cgaacgagcg
     4741 ccgagcagtt tgctactcgc ggccggttgg cgcgtcggcg cggatgtaga gctcttcaag
     4801 cgtgtgcccg gtcgtagccc acgccggcca gtggatcgcc tgaatgccat tgttgtccgg
     4861 ttccagcgca ggaccggtaa catatggctt gatcagctgg atcggcgtct ggaagtaggt
     4921 gaacaccgcc ggcacgtcct cgacgaggat gcgctcagct tcctggaaga tcgcgattcg
     4981 ctcctcctgc gaggcgagag aggtcgtgcc ctcctcgaca agcgcgtcgt actcctcatt
     5041 gctccaagag tgacggcccg tgctgtgcca gacaccgagc atgttggaag gatcgaagta
     5101 gtccatgccg taggagatga atccgaagag gatttcgtcc ggatcggcgt tcatggcggc
     5161 gtagaaggcc tgggggtcga gtgactggat ttggacgtcg atgttgagat gctcgctcag
     5221 catggacgcg taggcttccg ccgtcgcatc atagatcggg ttttggccga cgacgatgtt
     5281 catcgtgagt tccgggaacc cctccccgtc gggatagccc gcttcggcaa gcagctgccg
     5341 accgaagtcc gcatcgaaat tctggatgcc ggccagctcc tctgtgttcg aggcggggta
     5401 tcctggcgca aggaacgagg gggccgggtt ggcctgccgg ccccagacct gctgatcgag
     5461 cgcttcgcgg tccaagacgt ggctgaaggc ctggcgcacc tgcaggtcgt cgaacggcgc
     5521 cctctcgacg tcgaagaaga agtagaagca gcggaagtct cccacaccct gatagatctg
     5581 cgtcgtggtt tccgggtcct gctccatgat ctggagctcg gcgggagccg gtccctccat
     5641 atagtcgacc tgaccggcct cgtaaagggt gaagtggctt ggtggggcag ccagcttcac
     5701 gatgatctcc tgaagtgcca ctaccatgtc gccggtgtag gcctcgttgc gggtgtacac
     5761 gatctgctgg tcgcgcagcc attcctccag cttgaatggg ccggagctga cgtgggtctc
     5821 gggattggta ttgtagagtg ggccgtactc gtcgagcgca gcggcagaga gtggagttga
     5881 gtagaggagc ttggcggcga ggtacggtgc cggctcaatc gtctcgaaga ccacctcata
     5941 ctcgttcgcg ccctcgcgga caccaacttc ctcgaccggg acttccccag cggttgcctc
     6001 ctcgaagttg acgatatttc cgttccagaa ccaggtgaag tcccacgcgt gctcagggtc
     6061 ggcggcgtac tggaaggtgc gcacgtagtc ctgggcggta accgg
//
LOCUS       DASYWL010000045         3178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_264754, whole genome shotgun sequence.
ACCESSION   DASYWL010000045 DASYWL010000000
VERSION     DASYWL010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264754"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1439)
                     /gene="uvrC"
                     /locus_tag="VGR22_01570"
     CDS             complement(<1..1439)
                     /gene="uvrC"
                     /locus_tag="VGR22_01570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEV2127287.1"
                     /translation="MTTQHTDFSTRLSALAPAPGVYLMKNADGDIIYVGKAQSLRNRV
                     RSYFQSKRGMDPKTREMVSHVADFEVIRTDTATEALILENELIKKHQPKYNVRLRDSK
                     TYPHIRITNEDWPRIVSTRRIIKDGSRYFGPYTSAGAAYDTINLLNRLFPYRKCDKTI
                     TGNESVCLYYHMHQCTAPCIGAVDRETYMQAIEGARTFLEGRGDEIITTLEEEMDQAS
                     EAWNFERAAELRDRLQAVRHVLERQKIVSNPSTNADVIAVAKGVGGDAGVQVGFLRNG
                     KIIGSDFFQMQAAIDDKPSEIISSFLTQFYNEAAMIPSLILLQHSLPEGEAEMIRAWM
                     EQRREGTVDLRVPQRGLKRQLVAMIAESASENLEQSRLKFLSDEQKMTAAMTELADAL
                     DLPRLPRRIECYDISNLQGTNPVASMVVFEDGKPIKKEYRRFAIRTVTGANDFAMMAE
                     VITRRFKRAADQDEEQDGKWARLPDLVIV"
     gene            1628..2314
                     /locus_tag="VGR22_01575"
     CDS             1628..2314
                     /locus_tag="VGR22_01575"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEV2127288.1"
                     /translation="MPQEQQQKFAFAAFWLYKRSDRWYGLEDIDWERQCTETAEVVRE
                     TGEDVTLRGVYSSVGLNAHADMIIWAVSHQFEKLQQYAVAFDKTRLGRRMTMVESYIG
                     VGGMSLYDPTHGPAFLKGEEAKAYLSVYPFTKTPDWYLVPFEERRRLMIEHGELGREF
                     PTILTNTVNSFGIQDQEFIVALEDDDPHELIRMVQRLRAAEVRKYTAVDTPIYLGRRK
                     PIEEILEDLR"
     gene            complement(2636..>3178)
                     /locus_tag="VGR22_01580"
     CDS             complement(2636..>3178)
                     /locus_tag="VGR22_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2127289.1"
                     /translation="PLMSVSRNFFLGSEPMKGWGPFRHFDVATADEITRTELNRMGIA
                     IRDTSQPVGTLSGGERQSVAIARAVYFGARILILDEPTAALGVKEAGVVLRYIARARE
                     QGLGVIFITHNAHHAWTIGDTFTLLSQGESVGTFRKDETALEEVVTLMAGGAELEELE
                     HELGRNTPVSRHHTDANITT"
BASE COUNT          564 a          972 c         1019 g          623 t
ORIGIN      
        1 tcgacgatca cgaggtctgg caggcgggcc cacttgccgt cttgctcctc atcctgatct
       61 gccgcgcgct tgaaccgacg ggtgatgacc tcggccatca ttgcgaagtc gttggcgccg
      121 gtgaccgtcc tgatcgcgaa ccggcgatac tccttcttga tcggcttgcc atcctcgaag
      181 accaccatgc tggcgaccgg gttggtcccc tgcagattcg agatgtcgta gcactcgatg
      241 cggcgaggca ggcgtggaag gtcgagtgcg tcggccagct cggtcatcgc cgcggtcatc
      301 ttctgctcgt ccgagaggaa cttcaagcgc gactgctcga ggttctcgct cgcgctctcg
      361 gcgatcatcg ccaccagctg ccgcttcagg ccgcgctgcg gcactcgcag atcgacggta
      421 ccctcgcgcc gctgctccat ccacgcgcgg atcatctccg cctcaccctc gggcagcgag
      481 tgctgcagca ggatgagcga tgggatcatc gcagcctcgt tgtagaactg cgtcaggaag
      541 ctggagatga tctcgctcgg cttgtcgtcg atcgcggcct gcatctggaa gaaatcggac
      601 ccgatgatct tgccgttgcg gaggaacccg acctgcacgc cggcgtcacc acccacgccc
      661 ttcgcaacgg cgatgacatc agcgtttgtg ctcgggttgc tcacgatctt ctgccgttcc
      721 agcacatgcc gaacggcctg aagacggtct cgcagctcgg ctgcacgctc gaagttccac
      781 gcctcggagg cctgatccat ctcctcctcg agcgtggtga tgatctcgtc gcctcgacct
      841 tcgaggaacg tgcgcgcgcc ctcgatggcc tgcatatagg tctcgcggtc gaccgcgccg
      901 atgcacggcg cagtgcactg atgcatgtga tagtagaggc agaccgactc gtttccggtg
      961 atcgtcttgt cgcacttgcg gtaaggaaaa agccggttga gcaggttgat cgtgtcgtag
     1021 gcagcgcctg cggacgtgta gggaccgaag tagcggctgc cgtccttgat gatgcgacgg
     1081 gtgctgacga ttcgcggcca gtcctcgttg gtaatccgga tgtgcgggta tgtcttgctg
     1141 tctctcagcc tgacgttgta cttcggctga tgcttcttga tgagctcgtt ttcgaggatc
     1201 agtgcctcgg tcgcggtgtc ggtccggatc acctcgaagt cggcgacgtg cgacaccatc
     1261 tcacgagtct tggggtccat gccgcgcttc gactgaaagt aggagcgtac ccggttgcgg
     1321 agcgactgcg ccttgccgac atagataatg tccccgtcgg cgttcttcat gaggtagacg
     1381 ccgggggctg gagcaagggc ggagaggcgc gtggagaagt cggtgtgctg cgtcgtcatg
     1441 gcaacgcaca gtataccgtg cgggcagggc attctgggtg tcgccgcatc ggtgccctga
     1501 gggcggctga ggcaggcgtg ccgctgccgg ttggcgcccg atgaccacga ccgttcatta
     1561 ttgacggaac gaacggccga gtcacgtctg ggtcgactcg caccggcaac gaggaagtga
     1621 tcacgcaatg ccacaggaac agcagcagaa gttcgccttc gcggcgtttt ggctctacaa
     1681 gcgcagcgac cggtggtatg ggctcgagga catcgattgg gagcgacagt gcaccgagac
     1741 ggcagaagtg gtgcgggaga ccggggagga cgtcaccctc cgcggcgtct actcctctgt
     1801 cggcctgaat gcgcatgccg acatgatcat ctgggcagtc tcgcaccagt tcgagaaact
     1861 gcagcagtat gccgtcgcgt tcgacaagac gcgactcggc cgccggatga ccatggtgga
     1921 gtcgtacatc ggcgtcggcg gcatgtcgct ctacgacccg acgcacggcc cggccttcct
     1981 gaagggcgag gaggcgaagg cgtacctcag cgtctacccc ttcacgaaga cgccagactg
     2041 gtatctagtg ccattcgagg agcgtcgccg attgatgatt gagcacggcg agctggggcg
     2101 cgagttcccg acgatcctca ccaacacggt caactccttt ggcattcagg accaggagtt
     2161 catcgtcgcg ctggaggatg acgatcccca cgagctgatc agaatggttc agcgcctgcg
     2221 ggcggccgag gttcgaaaat atacggcggt cgatacgccg atctatctcg ggcgccgcaa
     2281 gccgatcgaa gaaatcctcg aggacctgcg gtagcttcac ggagcagacc tgatcaggtg
     2341 caagcagaca accctgtacg gcgatgtccg ggatacgcct ttggcgacat cgttcgatcg
     2401 ccaactccag cgttcaccgt ggcaacctca actcgtgatc ggccaggctt cggcgttgat
     2461 gtcattgttc tgcctgtggc gagcgacgga taacgccatc cacaggaggc ggcgcttcaa
     2521 tggggaaata gcgcggtaat gctgtagcgt cgggcacagg acccgacgtt ctcatcgttg
     2581 ccggtcatga tcgggccttc atccaaaggc cagccccacg cgtccaagtt tggaactacg
     2641 tggtgatgtt cgcatcagtg tggtgacgcg agaccggggt gttgcgtccg agctcgtgct
     2701 cgagctcctc gagctcggcg ccgccggcca tcagcgtgac gacctcctcc agcgccgtct
     2761 cgtccttccg gaacgtgccc accgactcgc cctggctcag tagcgtgaag gtgtcaccga
     2821 tcgtccaggc gtggtgggcg ttgtgggtga tgaagatgac gccgagtccc tgctcgcgcg
     2881 cccgcgcgat gtagcgcagc accacacctg cctccttgac ccccagcgct gccgtcggct
     2941 cgtctaggat cagaatccgc gcaccgaagt agaccgctcg ggcaatcgcg accgactgtc
     3001 gctcgccgcc ggagagggtg ccgaccggct gagacgtatc gcgaatggcg atgcccatgc
     3061 gattgagttc ggttcgggtg atttcgtcgg cggtggcgac atcgaagtgc cggaacggcc
     3121 cccacccctt cattggctct gagccgagaa aaaagttgcg cgaaacgctc atgagcgg
//
LOCUS       DASYWL010000046         6812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_658806, whole genome shotgun sequence.
ACCESSION   DASYWL010000046 DASYWL010000000
VERSION     DASYWL010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6812
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658806"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..1273)
                     /locus_tag="VGR22_01585"
     CDS             complement(167..1273)
                     /locus_tag="VGR22_01585"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2127290.1"
                     /translation="MSTGSRPVGIGIIGAGVISEIYLKNTTGVFDNIRAIAVADLVAE
                     RAQVRAKEFGIDALTVDELITHPDVEIVVNLTIPAAHHGVAKQVIEAGKSVYNEKPLA
                     INRDDARELVAIAKAKGVLIGGAPDTFLGGGLQTARKALEDGRIGTPVAATAHFLGRG
                     MEMWHPDPHFFFQPGAGPLFDVGPYYLTALTSMLGPVAAVTGFARASFPERTVTAEGP
                     KTGETIPVNTPTHIAGSLQFESGVIGSLIASFDVWETDERRTILTIYGSQGTLHLPDP
                     NRFGGPVTLVREQTRETEALPLTHGYTENSRGIGVSDMARALRGDTEPARASGEMAAH
                     VVDIMQSVLEAAESYSTVNLSTTMTRPAPLPAGG"
     gene            1630..2343
                     /locus_tag="VGR22_01590"
     CDS             1630..2343
                     /locus_tag="VGR22_01590"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2127291.1"
                     /translation="MKLIHVGLGNWGLDWEKSALPWVEETVERVAIVDAHEFTLATAQ
                     ERLALPDSTCFTALDDAFAATDAELVLITTPMVAHVPLAVEAMEAGKHVLVEKPFAGS
                     VREAQRAVDAAERTGRTLMVSQNYRFYPAPRTAAKIVASGMLGARGSVHIDFRRWAND
                     AEYEMNRHYQFPHPLLYDMSVHHYDLMRMVLGQEALEVYARDTSPSWSKFDEEAEALL
                     IVTFERADPQLSRQLGELR"
     gene            2330..3964
                     /locus_tag="VGR22_01595"
     CDS             2330..3964
                     /locus_tag="VGR22_01595"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEV2127292.1"
                     /translation="MSSGDPTHWAGNWRMEFSEGELSWTSRAGADIGAGGDLVQVRHR
                     GARPVTQSMEKLPLLGRSRALQHLVESIAAGVEPESSGRQNLGTVAIMEASVPRPRAT
                     ASSRSPRSGTCRDPREPRVAHLVHRCRYGRSEGCSGRPMTPGSSQPFSNHNGGGMNRQ
                     RVEVGLEVMLEANATPIDGKRVGLVTNSSAIDRQLRSAVDRLHADQRFDLATLFGPEH
                     GVRGDAQAGVEVDSVEDSRTGLPVYSLYGDTREPTPEMLDGLDALIFDVQDVPVRFAT
                     YISTLAYCQVAAASAGLDVVVLDRPNSLTGMHVEGNLLDPAFTSFVGIHPIVIRHGLT
                     VGEFARLFAAERSLPEPIVVPMRNWERDMWFDEMGLPWVQPSPNLPTLDSVTLYPGTC
                     LIEGTNISEGRGTARPFEYIGAPWIDPYRLAEDLERRNLPGLAYRPAFFSPVFSKHAG
                     EVCGGVQVYALDRKALRPVELGIQLLHALRGHNPDAFEWRSRSTGEYFVDLLSGTDRL
                     RRSLDDGAAPADIMADWDKDAAAFEERRRPYLLYPE"
     gene            complement(4029..6392)
                     /locus_tag="VGR22_01600"
     CDS             complement(4029..6392)
                     /locus_tag="VGR22_01600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV2127293.1"
                     /translation="MTHTAWRPDLVDALQQPIWTARADGTIIRANASWHAVTGIPAGI
                     TLGRWWDTAIHPDDIAPAAAGWDVGIRDSDPFEIEFRLRHAGGAWRWHLARIAPMPPG
                     EADEPAWVAIALDIDERRKAREARRASEVRLRELIANADDIIYTLGLDGMLTDINPAV
                     ERVLGYTPEEMMSLPFERIVVPDQVPYTRGMLERKLAGEPSSIYELDVIAKDGRRVTL
                     EINSRLIEEDGRPIAIHGVARDISNRRQRIQQAELSAAVGTALTTMQALGEQLQAAAQ
                     AIVDHLGVAFARIWLIDNDDPELLVLHASAGQYTHLDGAHSRIRVGEYKIGRIAARRE
                     PFLSNDVATDPEISDREWARREGMTAFAGYPLVLGDHLLGVVGLFARHRLDSTTQSVL
                     SSVTDTIAVAIERTYAEHARDTVLAREQEARVWAEVAETRYRNLFEGVADAILVADSE
                     RHYLDANAATSQLLGYTRAELLQMRVEDIVVAEPTWTESEFKQFVEDIVVAEPTWTES
                     EFNQFNEKGQWRGELALRHKDGTIIPVEARATVVRLPAGPVFMSAVRDITERKHLERL
                     QRDFLAMVTHDIRTPLTSVKGWMQLLQRRPYLEARDRRIVSRSLEQTETIAHLIDDLA
                     DLVRIEVGQLQLHRRQDDLLGIIREQIALVQEHAEHHRLRLETAEEAITGAWDRRRVG
                     QVLQNLLTNAVKYSADGGDVTVRVARAGDEARIEVEDQGIGISPDHLPLLFERFYREE
                     ATGAGGLGLGLHITRMLVETHGGAISAVSEQGTGSVFIVTLPIVSAA"
BASE COUNT         1062 a         2212 c         2336 g         1202 t
ORIGIN      
        1 cgccgaagcg ctcggcctgg cgccgcgcgt gttccggcac caggtcggcg atgccgacga
       61 cccgcaggtt ggcgaagcgc gtcgtgaagt tctccaggta gatggcggag atgtagcccg
      121 ccccgatgat gccgacgccg actggctcgt ggtccgtcac gttgcgctat ccacccgccg
      181 gcagcggggc cgggcgggtc atggtagtgc tcaggttgac ggtgctgtac gactccgccg
      241 cctccagcac cgactgcatg atgtccacga cgtgcgccgc catctcgccg ctcgcacggg
      301 caggttcggt gtcgccgcgc agcgcgcgcg ccatgtcgct cacgccgatg ccgcggctgt
      361 tttccgtgta gccgtgggtc agcggcagcg cctcggtctc gcgggtctgc tcgcggacca
      421 gcgtgaccgg cccgccgaac cggttcgggt ccggcaggtg cagtgtcccc tgggagccgt
      481 agatcgtgag gatcgtccgg cgctcgtccg tttcccaaac atcgaacgag gcgatcagcg
      541 agccgatcac gccgctctcg aactgcaggc tgccggcgat atgcgtcggc gtgttgaccg
      601 gaatcgtctc gcccgtcttc ggtccctcgg cggtgacggt gcgctccggg aagctggccc
      661 gggcgaaccc ggtcaccgcc gcgaccgggc cgagcatgct cgtcagtgcg gtcaggtagt
      721 acgggccgac gtcgaagagc ggtcctgcac ctggctggaa gaagaagtgt gggtccgggt
      781 gccacatctc catgccgcgg ccgaggaagt gcgcggtcgc cgcgaccggt gtgccgatcc
      841 tgccatcctc cagtgccttg cgggcggtct gcaggccgcc acccaggaag gtatccggcg
      901 cgccgccgat gagcacgccc ttcgccttcg cgatcgcgac cagctcgcgg gcatcgtcgc
      961 ggttgatcgc cagcggcttt tcgttgtaga ccgacttgcc ggcttcgatc acctgcttgg
     1021 ccacaccgtg gtgggcggcg gggatggtga ggttgacgac gatttccacg tccggatggg
     1081 tgatcagctc atcgacggtg agcgcgtcga tgccgaattc cttcgcgcgg acctgcgcgc
     1141 gctctgcgac gaggtcggcg accgcgatgg cgcggatgtt gtcgaagacg ccggtggtgt
     1201 tcttgagata gatctcggag atgacgccgg caccgatgat gccgatgccg accggtctgc
     1261 tgcctgtact catgctctcc cttggtcccc tgtgatgatt ggtgaagacg ccccattttt
     1321 gcgcgcctgc gatcactccc ggtaggatgc ccgaggcagg taagccgcgc aaggtgagca
     1381 cgctggcgcg cgatacggga ttccgctgtt tgtcaccagc ctgtcatttc gagccggcaa
     1441 ggcgcgtgag cgccgcagcc gtgtcgagaa atctcttgcc tggctggagt cgatgccccg
     1501 tgtggaaaag attcctcgac tgcaggctcg ctgtgctcgc cttccgctcg aaatggcacc
     1561 atggtttctg tcgccatcga cgaactcgtt tgtggtcggg catgtgcgcg acgggggagc
     1621 atcaaacgta tgaagctgat acacgtcggt ctcggcaact ggggactcga ctgggagaag
     1681 agcgcccttc cgtgggtcga ggagacggtt gagcgggtcg cgatcgtgga cgcgcatgag
     1741 ttcaccctcg ccactgcgca ggagagactg gcgctgccgg acagcacatg cttcaccgcg
     1801 ctggacgatg ccttcgctgc gaccgatgcc gagctggtgc tgatcaccac gccgatggtc
     1861 gcccacgtgc cgctggcggt cgaagcgatg gaggccggaa agcatgtact ggtcgagaaa
     1921 ccgttcgccg ggtcggtccg cgaggcgcag cgggcggtcg acgccgcgga gcggactggc
     1981 cggacgctga tggtcagcca gaactaccgc ttctaccccg cgccccgcac cgctgcgaag
     2041 atcgtcgcca gcgggatgct cggggcgagg ggcagcgtgc acatcgactt ccggcggtgg
     2101 gcgaacgatg ccgagtacga gatgaaccgg cactaccagt tcccgcaccc actgctctac
     2161 gacatgtccg tccatcatta cgacctgatg cggatggtgc tcgggcagga ggcgctggag
     2221 gtctacgcgc gggacacctc gccttcgtgg agtaagttcg acgaggaggc ggaggcgctg
     2281 ctcatcgtca ccttcgaacg ggcagaccct cagctatcgc ggcagctggg tgagctccgg
     2341 tgatcccacc cactgggccg gcaactggcg gatggagttc tcggagggcg agttgagctg
     2401 gacgagtcgc gccggtgcgg atatcggcgc tggcggcgac ctggtgcagg tgcgacatcg
     2461 cggtgccagg ccagtgacgc agtcgatgga gaagctgccg ctgctgggcc gctcgagggc
     2521 cctgcagcac ctcgtggaga gcatcgcggc gggtgtcgag ccggagagct ccgggcggca
     2581 gaacctcggc acggtcgcga tcatggaggc gtcggtgccg aggccgagag cgaccgcgtc
     2641 gagcaggtcg cccaggtcgg ggacctgtag agaccctcgt gaaccgcgcg tggctcacct
     2701 ggtccacaga tgccgatatg gccgctcgga gggctgctcc ggacggccca tgacgcccgg
     2761 ttccagccag ccgttctcta accacaacgg gggaggaatg aaccgacagc gagttgaagt
     2821 cggcctcgag gtgatgctcg aggccaatgc cacacctatc gatggcaagc gggtcgggct
     2881 cgtcaccaac agcagcgcca tcgaccggca gctgcggagc gcggtcgatc gcctccacgc
     2941 cgatcagcgc ttcgatctgg cgacgctctt cgggcccgag catggtgtcc gcggcgacgc
     3001 ccaggccggt gtcgaggtgg actcggtcga ggattcccgc acgggcctgc ccgtctacag
     3061 tctctatggt gacacccgcg agccgacgcc ggagatgctt gacggtctcg atgcactcat
     3121 cttcgatgtc caggacgtgc ccgttcgctt cgccacgtac atctcaacgt tggcctactg
     3181 ccaggtcgcg gcggcgagcg ccggtctgga cgtcgtcgtc ctcgatcgcc cgaactcgct
     3241 gaccgggatg catgtcgagg gcaacctgct cgaccctgcg ttcacctcat tcgtcgggat
     3301 ccacccaatc gtcatccggc acggactgac ggttggagag tttgcgcggc tcttcgctgc
     3361 ggagcggagc ctgccggagc cgatcgtcgt tccgatgcgt aactgggaac gtgacatgtg
     3421 gttcgacgag atgggacttc cctgggtgca gccttcgccg aacctgccca ccctggactc
     3481 cgtcaccctg tatcccggaa cctgcctgat cgagggcacc aacatctccg aaggccgggg
     3541 cacagcccgg cccttcgagt acattggtgc gccgtggatc gatccctaca ggctcgctga
     3601 ggatctggag cgccgcaacc tgccgggcct cgcctaccgc ccggccttct tctcaccggt
     3661 cttttccaag cacgccggtg aggtatgcgg cggcgtgcag gtctatgcgc tcgaccggaa
     3721 agccctgcgt ccggtggagc tcggcatcca actcctccac gcattgcgcg ggcataaccc
     3781 cgacgcgttc gagtggcggt cgcggtccac aggcgagtac ttcgtcgacc tgctgtccgg
     3841 cactgacagg ctgcgtcggt cgctcgatga tggggcggcc ccggcggaca tcatggccga
     3901 ttgggacaag gatgcggcag cgttcgagga gcgccgccgg ccgtacctgc tgtacccgga
     3961 ataacgaagc tcccctgaac tatcggggga cccaggtgga ggggcgagcg aaacactcgt
     4021 cgggatcgtc atgctgccga gacgatcggc agcgtgacga tgaagacgct cccagtgccc
     4081 tgctccgaga ccgccgagat cgccccgccg tgcgtctcga ccagcatccg tgtgatgtgc
     4141 aggccgagcc ccagcccgcc ggcgccggta gcctcctcgc ggtagaaccg ctcgaagagc
     4201 agcggcaggt ggtccggcga gatcccgatg ccctggtcct cgacctcaat tcgtgcctca
     4261 tcccccgcgc gggcaactcg caccgtcaca tccccgccgt ctgcgctgta cttgaccgcg
     4321 ttggtgagca ggttctgcag cacctggccg acgcgtcgtc ggtcccacgc gccggtgatc
     4381 gcctcttcgg ccgtttccag gcgcaggcgg tggtgctcgg catgctcctg caccaaggcg
     4441 atctgctcgc ggatgatgcc caagaggtca tcctggcgcc ggtggagctg cagctgcccc
     4501 acctcgattc gcaccagatc ggcgaggtcg tcgatcagat gggcgatcgt ctcggtctgc
     4561 tcgaggctcc ggctcacgat ccggcggtcc cgggcctcca gatagggccg ccgctgcaga
     4621 agctgcatcc accccttgac tgaggtcagc ggcgtgcgga tatcatgggt gaccatcgcg
     4681 aggaaatcgc gctgcagccg ctccaggtgc ttgcgctcgg tgatgtcgcg cactgccgac
     4741 atgaagactg gaccggccgg tagacgcacg acggttgccc gggcctcgac ggggatgatg
     4801 gtgccatcct tgtggcggag cgccagctca ccccgccact ggcccttttc attgaactga
     4861 ttgaactcgg attccgtcca cgtgggttcg gccactacga tgtcctcgac gaactgtttg
     4921 aactcggatt ctgtccacgt gggttcggcc actacgatgt cctcgacccg catctggagc
     4981 agctcggcac gcgtgtagcc gagcagctgc gaagtcgcgg cgttggcgtc cagatagtgg
     5041 cgctcgctgt cggccacgag aatggcgtca gcgactccct cgaagaggtt gcggtatcgg
     5101 gtctccgcca cctctgccca gacgcgcgcc tcctgctcgc gggccaggac ggtatcgcgg
     5161 gcgtgctcgg cgtacgtgcg ctcgatggcg acggcgatgg tgtcggtcac ggaactcaag
     5221 accgactggg tggtggagtc gagccggtgg cgggcgaaga gcccgacgac gccgagcagg
     5281 tggtcgccca gcacgagcgg gtagccggcg aacgcggtca tcccctcgcg gcgggcccac
     5341 tcgcgatcgc tgatctccgg gtcggtggcg acgtcgttgg agaggaaggg ctcgcgccgc
     5401 gcggcgatcc gtccgatctt gtattccccc acgcgaatcc ggctgtgcgc cccgtccagg
     5461 tgtgtgtact gcccggcgct ggcatggagc accaggagct cgggatcgtc gttgtcgatc
     5521 agccagatgc gcgcgaacgc cacgccgagg tgatcgacga tcgcctgtgc cgccgcctga
     5581 agctgctcac cgagcgcctg catggtggtg agcgcggtac cgaccgcggc gctcagctcg
     5641 gcctgctgga tccgctggcg tcggttgctg atgtcccggg cgaccccgtg aatcgcgatg
     5701 ggccggccat cctcctcgat caggcgggag ttgatctcca gcgtcacacg tcggccgtcc
     5761 ttggcaatca catcgagctc gtagatgctg ctcggctccc cggcgagctt gcgctcgagc
     5821 atgccccgtg tgtatggcac ctggtccggt acgacgatcc gctcgaacgg cagggacatc
     5881 atctcttcgg gggtgtaccc cagcacgcgt tccacggcgg ggttgatgtc ggtcagcatg
     5941 ccgtccaggc cgagcgtgta gatgatgtca tccgcgttgg cgatcagttc ccgcaggcgg
     6001 acctcgctgg cgcggcgagc ctcgcgtgcc ttacggcgct cgtcgatgtc gagggcaatg
     6061 gccacccatg ctggttcatc cgcttcccct gggggcatgg gtgcgatacg agcgaggtgc
     6121 cagcgccagg cgccaccagc gtggcggagg cggaactcga tctcgaacgg atcagaatcg
     6181 cggatgccca catcccatcc tgcagcagcg ggggcgatat cgtccgggtg gatggcagta
     6241 tcccaccagc ggccgagcgt gatccccgcc gggatgccgg tgacggcgtg ccaagacgcg
     6301 ttggcgcgga tgatcgtgcc gtcggctcgg gcggtccaga tcggctgctg gagcgcatcg
     6361 acaaggtcgg gccgccatgc ggtgtgggtc atgctggtga gcacctctct gattgggcgg
     6421 ttccggtctc gtagcgatgt gtacgcgcgg gcggtcggcg gagaagtgcc gcgtggatcc
     6481 ctcccgcgct atgcagttcc gcacgacagc ggccaccgtc cggcacgtct ggtactgccg
     6541 tatcaccaca tggaaagagg ccggacacgg caacacccgg ctgggaacag ggggcaagag
     6601 tccccagccg ggtgttgcga gctgtggacg gaacggaggg ggcccggcag gtcctctacc
     6661 ggtctctcgt gccgttgcta taaacagtag cgggtgccga ccggtgcaaa cctgccattc
     6721 acctggcagt ctcgtagcac ctaggcataa cacttgctgg gctaggtgga gacgtactgc
     6781 gttgggggca caatcacatt caggcagcct gc
//
LOCUS       DASYWL010000047         1543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_283471, whole genome shotgun sequence.
ACCESSION   DASYWL010000047 DASYWL010000000
VERSION     DASYWL010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283471"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="VGR22_01605"
     CDS             <1..1033
                     /locus_tag="VGR22_01605"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2127294.1"
                     /translation="AYGVPHALTNSDDLIARDDVDLVLVITPIGLHYPLALQALHAGK
                     HVYVQKTMTTAYGEAKDLVDTARELGLVLVAAPGQMLAPAMRAMRSLVAKGALGDVYW
                     AWGSTGGWQHHLEANRQGDDARSSADPSWYYTKLGGPLRDVTVYVLHSLTGILGPAKR
                     VAAMSGIRTKEHRWRDKVIPVEVDDNTMLMLDFGDATFAMLGGHSAATGKLIQWGGMG
                     IYGSRGAVETLEIEPLSGHPVALAVNGDVDLPELEPAGSGTYTLRTHLPHVTERHAAI
                     PEPHVYADIMHCVDCIRTGAPPVASGEHAAHVVEIIEKGYLAAETGQAQQLESTFSMA
                     ELSTGGSIL"
     gene            1030..>1543
                     /locus_tag="VGR22_01610"
     CDS             1030..>1543
                     /locus_tag="VGR22_01610"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2127295.1"
                     /translation="MTQRLKLGLLGCGDVAQRDYLPELHRLQDRVELVAVCGRSEARA
                     RAVAEQYGVDAWFTDYRLMLERGEAEAILNLTPIQLHAETTLACLEAGKHVYSEKPAA
                     TTVADAARLREVANDRSVVLVCAPSILLYPQIQEARRLLERQVIGSVHSAVGQAFGGV
                     PPWSGYTSDPS"
BASE COUNT          257 a          501 c          529 g          256 t
ORIGIN      
        1 cgcctatggc gtcccgcacg cgctcaccaa ctccgacgac ctgatcgccc gcgacgacgt
       61 cgatctggtg ctggtgatca cgccgatcgg cctgcactat ccgctcgcct tgcaggcgct
      121 ccatgcgggc aagcacgtct atgtgcagaa gacgatgacc acggcctacg gagaggcgaa
      181 ggacctcgtc gataccgcgc gcgagctggg cctcgtcctc gtggcggcac cgggccagat
      241 gctcgcgccc gcgatgcgcg cgatgcgctc gctcgtggcg aagggcgcgc tgggcgatgt
      301 gtactgggcg tgggggagca cgggtggctg gcagcaccat ttggaggcca accgccaagg
      361 cgacgatgcc cggagttcgg ccgacccgtc ctggtactac acgaagttgg gagggccgct
      421 ccgggatgtc acggtctacg tgctgcacag cctgacgggc attctgggtc cggcgaagcg
      481 cgtcgctgcg atgtccggca ttcgcaccaa ggagcatcga tggcgcgaca aggttatccc
      541 cgtggaggtc gacgacaaca ccatgctgat gctcgacttc ggcgacgcga cgtttgccat
      601 gctgggcggg cacagtgccg ccacgggaaa gctgattcag tggggtggga tggggatcta
      661 cggctcgcgc ggcgcggttg agacgctcga gattgagccg ctctccggcc acccggtcgc
      721 gctggccgtc aacggggatg tcgaccttcc ggagttggaa cccgcggggt cgggcaccta
      781 caccctccgc acccatctcc cacatgtcac cgagcggcat gcggctattc ccgagccaca
      841 cgtctatgcc gacatcatgc attgcgtcga ctgcatccga accggtgcgc cgccggttgc
      901 ctccggcgag catgccgccc atgtcgtgga gatcatcgag aaggggtatc tggcagcgga
      961 gacgggtcag gcgcaacagc tcgagagcac cttctccatg gcggaactga gcaccggggg
     1021 atcgatcctg tgacacagcg tttgaaactg gggttgctcg gctgtggcga cgtggcccag
     1081 cgggactacc tgccggaact gcaccgcctg caggaccgtg tggaactcgt tgccgtgtgc
     1141 gggcgctccg aagcgcgcgc ccgggccgtc gctgagcagt acggcgttga cgcctggttc
     1201 accgattacc gcctgatgct cgagcggggg gaggcagagg cgatcctcaa tctgacaccg
     1261 atccagctgc atgccgagac cacgctcgcc tgtctcgagg cagggaagca tgtctatagt
     1321 gagaaacccg ccgcgacgac ggttgccgat gccgcgcgcc tgcgcgaggt ggcgaacgac
     1381 cggagtgtgg tgctggtctg cgccccgtcg attctgctct acccgcaaat ccaagaagcc
     1441 cggcgcctcc tcgaacggca ggtgatcggc tccgtgcact cggcggtcgg gcaggcgttc
     1501 ggcggcgtgc caccctggag cgggtatacc tccgatccaa gcc
//
LOCUS       DASYWL010000048        20164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_208522, whole genome shotgun sequence.
ACCESSION   DASYWL010000048 DASYWL010000000
VERSION     DASYWL010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 20164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20164
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208522"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..343)
                     /gene="rpmB"
                     /locus_tag="VGR22_01615"
     CDS             complement(149..343)
                     /gene="rpmB"
                     /locus_tag="VGR22_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEV2127296.1"
                     /translation="MAGKCDICGKTKSFGNNVSFSHKKTNRDFRPNVQKKRIVIDGEA
                     KRLNVCTRCMRTLGKSGKAA"
     gene            639..1025
                     /locus_tag="VGR22_01620"
     CDS             639..1025
                     /locus_tag="VGR22_01620"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV2127297.1"
                     /translation="MTSPGPDKPRQPGGTVEISDHAIVAVVHDAVLSCYGIVDMAPRS
                     IRSAIGKRFGLGTTQRGIGITQDEGRLTIDLSVVVEYGTPIFTVATNVMQTVKFRVEE
                     LLGMEVDRVNIHVDGLHVSDEAGRAS"
     gene            1022..2704
                     /locus_tag="VGR22_01625"
     CDS             1022..2704
                     /locus_tag="VGR22_01625"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEV2127298.1"
                     /translation="MNSTTSSQAVWNGQALRDAFTFASALIQRNRDRVNQLNVFPVPD
                     GDTGTNMALTMTGALEVLEDLPETATASEVANRVAYGALMGARGNSGVILSQIFRGFA
                     NGLNGVTELSGGDLAKALDGARETAYKAVMRPVEGTMLTVIRGAAEGASEAAKTHRSV
                     TEVLRAAHEAAQVALDGTPQQLDILRQAGVVDAGGQGVVIILEALLRSATGDTELPTM
                     EEAGAVGGDMAFLDMVEETHGEDTFGYCTNFMVLGSDIDAEKARDEIAAMGESAVIVG
                     DDSMLKVHIHTLDPGGVLQYGIRLGELDQIKIDNMSKQTETLQSQRAGTLTQNGAPPP
                     EPEGEQLHNDLAIIVVAAGEGISTALRSLGATSIVQGGQTMNPSTQELLAAVEDSAAE
                     EIILLPNNKNIHMAANQVAELSEKTVRIVPTRSIPAALAALTAFNTDQSLDENVDDMA
                     EAMTDTTAIAITRAVRDAEISGIAVATGQTIGLINDELAAAGDDELQVVLDTFERANV
                     DDPELLTIFVGESVPDDEAETLQERLGETWPDAEIEMHNGGQPHYRYIISAE"
     gene            2758..3618
                     /locus_tag="VGR22_01630"
     CDS             2758..3618
                     /locus_tag="VGR22_01630"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV2127299.1"
                     /translation="MAETPRIAIVTDSTADLPEDLLTRLGITVVPLNVHIGGESFRDQ
                     IDLTTDEFMGRLADTRDLPSTSQPSAGIFERTFREIGQDHDAIVCVLISSRLSGTVQS
                     AQVAADAVGDVARVEVIDSLSASLGCGFQVLHAHRLADQGLGAKEIATRLRSQTMNYH
                     VVFFVETLDHLRRGGRIGKAASLVGSMLKLKPLLRIDEGQVVPFERTRTRKRAIEALV
                     EFAQNMPEIERIGVLHNTTPEDAERLSERVQSLARRESEVPIAKFGPVLGTHVGPGTL
                     GLAIEVANNA"
     gene            3611..4459
                     /locus_tag="VGR22_01635"
     CDS             3611..4459
                     /locus_tag="VGR22_01635"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV2127300.1"
                     /translation="MPDAQHVTIVTDSTADVPPDMREALEIEVVPLRVNFGQESFRDG
                     IGLTTAQLMERMANAPSLPTTSQPTAGEFAAVFQDAIDAGREVVCITIGAKLSGTYQA
                     AVTAAEQVALDRIEVIDGATTTMHIGWPAVAGARVARQGADRETVSRTIRDARERSNL
                     FVALKTLDNVYKGGRIGKASQLVGSALGIKPIVSFRDGTLVPIERVRTWKKAVRRMVE
                     LVVEEGDLSDIIVVYTDDPTDAETIQAELREHYPNANVILGTAGAVIATHAGPGAIGV
                     ATLRRA"
     gene            4522..7032
                     /gene="recG"
                     /locus_tag="VGR22_01640"
     CDS             4522..7032
                     /gene="recG"
                     /locus_tag="VGR22_01640"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEV2127301.1"
                     /translation="MASRTHQALSPTSRAEQHYEQTLRALRVLWTARDSARPEMREAV
                     AAYRQLIPAAAATHEMQDMLQRSIERLERLPMDLESRRPIVETLAAELKALRPRLGLV
                     EPTGEKGVLNIAAGERRAGRDSPAQNKPRTPSTQERLPIDAAVTELNGVGKKQAAMLD
                     KLGIHTVGDLLRLSPRRYVDYRNPIKIGQHLDFTGHILVRGTVSNIREHRTRKGPSRV
                     TMELSDGTGVLTLTFFSPYIARQVREGSEIYAAGTVEHGYGKLQMVSPEWDLVGGPNV
                     ATGHLVPVYPLTKGLYQKNMRRFTRLALDATRPNLVDWLADIHDYIDDAVWQRMPSLE
                     QALEHLHYPPDLHAATAAVRRMTLESLILLQLGLVKRRQEASATPGTAFTFDRDRLDR
                     FIDELPFELTGAQRRSLDQIIADITRPHAMTRLLQGDVGSGKTVVAAAIALVAAASGH
                     QTAVMTPTELLAEQHYRNLTAQYAALHEDEQPSVALLTGSITAKTRRELLTRLAEGEI
                     DVLIGTHALIQDDVEFRALGLVVIDEQHRFGVRQRSTLAAKARGTLPHLLSMTATPIP
                     RTLNLVLYGDMNVSIIDERPPGRIPIETRHYIGSERDRAYAQVREQVAMGHQIFVICP
                     LVEESDTLEAKAAVTEAERLQNEVFPELQVEVLHGRMPSKKKDEVMAAFRDHQFDILV
                     STSVIEVGIDIPNATVMMIEGADRFGLSQLHQFRGRVGRGGSKSYCLLLADTVTLEGQ
                     IRLDTMVATDDGFELAQKDLELRGPGDFLGGTRQSGLPDLGFLVQGFDTRLLDLARST
                     AEAVLAEHLDITWQAFPHLYPQYRAFWATAGTPDEGKS"
     gene            7275..7871
                     /locus_tag="VGR22_01645"
     CDS             7275..7871
                     /locus_tag="VGR22_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HEV2127302.1"
                     /translation="MRVISGSARGRKLKGPPSHATRPMADKIKGALFNVLLSLDVQPD
                     RILDLYAGTGNIGIEALSRGGSWADFVDKGRDQAKVIRQNLETTGFSDRAKVHQIPVE
                     TFIATAHGQPYDFIILDPPYADPSIVETVESIGNSRLVQSGTIVVIGHWPRLEVPDTV
                     GRLECLRNRCHGDSCFSVYEVVDVSGSAPSGEEQQKTP"
     gene            7868..8398
                     /gene="coaD"
                     /locus_tag="VGR22_01650"
     CDS             7868..8398
                     /gene="coaD"
                     /locus_tag="VGR22_01650"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEV2127303.1"
                     /translation="MRLAVYPGSFDPITNGHMDVALRAAKLFDGLIVSIYSGTTKANA
                     LFDPEERIELAKQAFAESDEPQASRIRVESFSGLAVEYARSVGATTLVRGLRAVSDFE
                     YEFKYAHMNNHIAPEIEVVCLMTSSKHSFISSSFIREVASLGGTVDGLVPPNIAAAVH
                     RRVSAVNHPLPAHGMA"
     gene            8431..8961
                     /locus_tag="VGR22_01655"
     CDS             8431..8961
                     /locus_tag="VGR22_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127304.1"
                     /translation="MDDTIERPANQDGAVDMLYLVDRLEELVSLGKRVPFSTRVMVEE
                     DEFLALVDQLRVAVPNEIKQAQRVIKERERIIGDAQDEAADIVGRAHEEADRMVSQSA
                     LLAEARQRGEDILRRAEEEQQRTKGELDVYVLEQAQLMEDAIQRAIAIMQDAAEESLD
                     SIIELKAEVSRASDRR"
     gene            complement(9107..9370)
                     /locus_tag="VGR22_01660"
     CDS             complement(9107..9370)
                     /locus_tag="VGR22_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127305.1"
                     /translation="MRALYFVLAGYLADLKYLKPGLAGVLVFVGGEMLLIDVYKIPAL
                     GSLAVIITILAIAFGASAIARRREVAAGDGEAALPPGTNPVAE"
     gene            complement(10226..11026)
                     /locus_tag="VGR22_01665"
     CDS             complement(10226..11026)
                     /locus_tag="VGR22_01665"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF006673.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEV2127306.1"
                     /translation="MEGSKSLNVTVRKSLGLYANVRPCVAYAPFVPTRHPGMDVVIVR
                     ENEEDLYAGIEHCQTDEVVQCLKLISRPGCEKIVRYAFEYARRNNRKKVTCFTKDNIM
                     KLTDGLFHRVFDEIAEEYPELEHEHWIVDIGTAKLADTPEVFDVIVTPNLYGDVLSDV
                     AAQIAGSIGLAGEANVGEHGAMFEAIHGSAPRRAGQDIANPSGLLLAGVMMLVHVGQS
                     DVAERIHNAWLRTIEDGVHTYDIFDESVSKQQVGTREFAREVIERLGQ"
     gene            complement(11810..12421)
                     /locus_tag="VGR22_01670"
     CDS             complement(11810..12421)
                     /locus_tag="VGR22_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127307.1"
                     /translation="MAKRILVPGVVVAMFTLLASLVTMAGSVTAQATPPGVATPAVPF
                     EAEIELHPAHIHAGTCQDLGDIVYPLNDLQEAAAVREPDATPPLDLAATPDAEEVVGL
                     VGLTAQSSTEVDATLEELLAEDFAINVHESEENIMNYLACGNITGTPDEGELFIDLHE
                     IDNSGFAGQAELADQGDGTTFVHVTIFPKELPGGTPVDATPTS"
     gene            12611..13267
                     /locus_tag="VGR22_01675"
     CDS             12611..13267
                     /locus_tag="VGR22_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEV2127308.1"
                     /translation="MTEHQQDPAAARPAASSTPATPRPSPDEALELLMAGNRRYVEGA
                     ITQDYSARRTALKEHAPIAAILGCGDARVGAELIFDRGPGDLFMVRVAGNFLSDYGLA
                     SLEFCVEFLQVPLLMVLGHTHCGAVTAAVDVVEKRQDLPGRLFVLIDALEPSVLRAKM
                     DEPADLLLAATEENVRRQVRRLETISPVINAAREAGRVKIAGGIYDIDTGKVTLLDQS
                     "
     gene            complement(13340..15190)
                     /locus_tag="VGR22_01680"
     CDS             complement(13340..15190)
                     /locus_tag="VGR22_01680"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEV2127309.1"
                     /translation="MARSFNNASLASIVLQGIDRPLPAIRGWSRDRVRHLRHTVLPES
                     GGDPDLQPETVPVIAPPQGEQRFRMVLVRRIFLAVLVALLVVAVGLVVFRGAYAGRVY
                     PSIVVGDVPVGGLTIEEAKARLTERAADLERGTVVFSHDGQTWTPSLAELGVSVNLDA
                     SLAQAEQLGRDGNATTRLGFVAEVLGTDQVVPLQTQLDQRALDLWFDSVERDVGQLPV
                     DARIEVRDGEVVVVPGEAGIVVDREAAIAYIQQALSTLEPIADDLPMQVGLPALNAEA
                     LAPIERRVTEVISTPITARVLDEEWQITGETVGSYLTIEVVMESGEPTAHLAVDTDGL
                     SDELRAQFADKVNRKPVSAVLGWDNGVVAIEPSVTGAAMKADAFAEVVATSFLGSHEP
                     VDIPVVLIEPEIDDTKLESYGITELLGTGHSNYVGGTWERDENIRVAISLLDGALVPP
                     GGTFSFNGAIGEITADKGYQEAIVVSGDRVGRDVGGGVCQVSTTVFRAALNAGMRIEE
                     WYPHTFRLPNYERDGWGPGFDASILQYGPNPEEWPDFRFENYSDSWLLIEAYMTDSQV
                     FVNIYGTGDGRAVEISAWQVDKNTFAFSRAVRDAQGSVIDEQIFESPFKG"
     gene            complement(15446..15958)
                     /locus_tag="VGR22_01685"
     CDS             complement(15446..15958)
                     /locus_tag="VGR22_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127310.1"
                     /translation="MGVLDWLTSTKRPEPGVAACSADEVRERILAINRPTAPFQIVDG
                     SDEGVNLIAEWKIVDAQWYEIFAKAGLEKVFRISMKLDDKEKQVRTKDHEYTVFWEAG
                     VPHLSFSASKTMGQKQSVQFGTAYAFTEELRPGQVYNYRFSTGEMKKSIQESVAGCGW
                     TYKGIVFGKL"
     gene            complement(16033..17181)
                     /locus_tag="VGR22_01690"
     CDS             complement(16033..17181)
                     /locus_tag="VGR22_01690"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2127311.1"
                     /translation="MNTLRVGIIGVGWPGQRHIEGYQKHPDVQIVALSDLNTAAAEQV
                     KAEYNVQGAEIFGDYQELLASDHLDAVSICTPNFLHASMAIDALDAGKHVLLEKPLAR
                     TLAEGERLAEKVAAHPDQVFMIALNNRYRPDSVVLKQHLDAGELGDIYYAKTGWLRGA
                     SHFFIRDRSWFTQRERSGGGPLIDLGVHMLDLSLWFMGNPRPVSVSGAVYYTFNEFMR
                     DSTGAEVDVEDLATAFIKLDNGATVMLDVSWLSHIEEGNLVYSQLFGTNGGAKMQRGP
                     AEDLKIVTTSGTGATRTTMVSTPEFHSPAAQQPGFQLYESFRAEIADFVDSVRDHRQP
                     GATITHGLDILRVLDGIYRSAETGQETDLHVPTASEAVVEPGEVLPSS"
     gene            17465..17764
                     /locus_tag="VGR22_01695"
     CDS             17465..17764
                     /locus_tag="VGR22_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127312.1"
                     /translation="MMGRHGSTASPSPWVASGLSVLVEGFGQAYNRQPVKAAVFLITG
                     LTLSTGSGLNTWLAQNILGLKGTRLGPERVRPGLLGLWFATFVLNLFDAWRTARR"
     gene            complement(17844..19106)
                     /locus_tag="VGR22_01700"
     CDS             complement(17844..19106)
                     /locus_tag="VGR22_01700"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEV2127313.1"
                     /translation="MSEKNRREAMEDSSSILRAGNRDEREGIALCLSGGGFRAALYHL
                     GALRRLNELGILGDVATISAVSGGSILAAHLVERIPEWPAKGDVVDDWDGQVAQPFRD
                     ITSQNLRTGPVLRRFLPWNWTRPEAGIRALARRYQRQITSARLGDLPDRPRYIFCATD
                     MVYGRFWVFEKRGAGRFDTELAPYPKWPVARAVAASSCFPPIFAPMAVAMSPGPDGRQ
                     AGTARPPKRKVDRGHLTDGGTYDNTGVEAVWEDHATILVSDGGGTFDTRWRNSWLWRL
                     RRYPAIANARVRVVQKRWLFASFRAGLFDGTIWGIDEPKWDLQEIGSPTVYPRDLVET
                     VIAQIRTDLDAFSEAEQKVLENHGYLTAEAAIRALTPSLPKREAPALPPYPEWMDPDK
                     VREALADSDQRRMLGRPYASHPLDLLFE"
     gene            19603..>20164
                     /locus_tag="VGR22_01705"
     CDS             19603..>20164
                     /locus_tag="VGR22_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127314.1"
                     /translation="MPELVPDPKLELSVDAVFGYAMAAYYGGRAECRVREVPVPVAYC
                     DFRSMYPSVNTLMGLWRFLTAQRLEVVDATAWAQNFLARTTADDCFRRATWEQLAALV
                     EVEPDGELLPVRAPYSAEQAGYTIGVNPLRATEQVETIWYTLADCLAAKIATGRAPRV
                     RRALRFVPKGRQRGLHPVDLRGQVTVD"
BASE COUNT         3647 a         6715 c         6333 g         3469 t
ORIGIN      
        1 tgagccgagg cccggctacg ccgggcctcg gctcaggatg aaggctagct gtcgccacat
       61 tatcgtcttg ccttcaagga caacaagaac ccccggtcgc aggtgcgacc gggggtttcc
      121 tggcagtctt ggtaccggct atgcccgcct aggctgcctt gccggacttg cccagcgtgc
      181 gcatgcagcg cgtgcagacg ttgaggcgct tcgcctcgcc atcgatcacg atccgcttct
      241 tctgcacgtt gggccggaag tcacggttcg tcttcttgtg tgaaaagctt acgttgttgc
      301 cgaacgactt ggtcttgccg caaatgtcac acttcccggc cacgtttgca ctcccttcgc
      361 tggtttcctg ctgtactggt ccgcgtcggg ccaataccga cgaaagagcc gggcccggac
      421 actgtcacct tgttgccgcc cggatggtga ggatcgcccc gggcacggaa caacgtcgcc
      481 acgccagatg gcggcgtaac tccgatcacg tatcgtacca ttaggggata tgatcggcaa
      541 ttcccgagca tgtcaggcgc aacatgcctc acgattgccg ggatgccatc ggcccgaccg
      601 ttccatgccc ccgaccgacc agcagggagc aatctgtaat gaccagtccg ggtcccgaca
      661 aaccccgcca gcccggcggc accgtagaga tatcggacca tgccatcgtc gccgtggtcc
      721 atgacgccgt gctgtcctgc tatggcatcg ttgacatggc gccacgctcg atccgttccg
      781 ccatcggcaa gcgcttcggg cttggcacaa cccagcgcgg catcggcatc acccaggacg
      841 agggccggct gaccatcgat ctctccgtcg tggtggagta cggcacgccg atcttcaccg
      901 tggccaccaa cgtgatgcaa acagtcaagt tccgtgtcga ggagctgctg ggcatggagg
      961 tcgaccgggt gaatatccac gtcgacggcc ttcacgtcag cgacgaagcc gggagagcgt
     1021 cgtgaactcg accacatctt cccaagcggt atggaacgga caggcgctgc gtgatgcctt
     1081 caccttcgct agcgcgctga ttcagcgcaa ccgcgaccgc gtcaaccagc tgaacgtctt
     1141 ccccgtaccg gacggcgaca cgggcaccaa catggcgctc accatgacgg gcgcgctcga
     1201 ggtactggag gacctgccgg agaccgccac agcctccgaa gttgccaacc gggtcgccta
     1261 cggcgcgctc atgggtgcgc gcggcaactc cggcgttatt ctctcgcaga tcttccgcgg
     1321 ctttgccaat ggactcaacg gcgtcactga gctgagcggc ggcgatcttg caaaggcgct
     1381 cgacggagcc cgggagaccg cctacaaggc ggtgatgcgc ccggtcgagg gcacgatgct
     1441 gacggtgatc cgtggtgcgg cggagggagc ctctgaagct gcgaagactc accgctccgt
     1501 cacggaggtg ctgcgcgccg cgcacgaggc tgcacaggtt gcgctcgacg gcacgccaca
     1561 gcagctcgat atcctgcgac aggccggcgt ggtcgacgcc ggcggacagg gcgtcgtcat
     1621 catcctggag gccctgctgc gcagcgccac cggcgacaca gaactcccga ctatggagga
     1681 ggcgggcgcg gtcggcggcg acatggcgtt cctcgacatg gtcgaggaga cccatggcga
     1741 ggacaccttc ggctactgta cgaacttcat ggtcctcggc tccgatatcg acgccgaaaa
     1801 ggcgcgcgac gagatcgcgg cgatgggcga atcggcagtg atcgtcggtg acgactccat
     1861 gctcaaggtc cacatccaca cgctcgatcc cggcggcgtg ctgcagtacg ggattcggct
     1921 gggcgagctc gatcagatca agatcgacaa catgagcaag cagaccgaaa cgctgcagag
     1981 ccagcgcgca gggacgttga cgcagaacgg tgcgccgccg cccgagccgg agggcgagca
     2041 gctgcacaat gacctcgcga tcatcgtcgt cgccgccggc gagggcatct ccacggcgtt
     2101 gcgctcgctt ggcgcgacga gcatcgtgca ggggggccag accatgaacc ccagtacgca
     2161 ggaactcctc gccgcggttg aggactccgc cgccgaggaa atcatcctgc tgccgaacaa
     2221 caagaacatc cacatggccg ccaaccaggt agccgagctc agcgagaaga ccgtccggat
     2281 cgtacccacc cggtccatcc ccgccgcgct cgccgcgctg accgccttca acactgacca
     2341 gtcgctggac gagaacgtgg acgacatggc ggaggcaatg acggacacga cggcgatcgc
     2401 gatcacccgc gcggtacgtg atgccgagat cagtggcatc gccgtcgcca ccggccagac
     2461 gatcggtctg atcaacgacg aactcgccgc cgccggtgac gacgagcttc aggtcgtact
     2521 cgacaccttc gagcgcgcca atgtcgacga tccggagctg ctcacgatct tcgttggcga
     2581 gagtgtcccg gatgacgagg ccgaaaccct gcaggagcgg ctcggcgaga catggcccga
     2641 tgccgagatc gaaatgcata atggtggcca gccgcactat cgttacatca tctcggccga
     2701 gtgacgcgac gacccggagg agagcgggct ccgagatgat ccggagagaa catcctcatg
     2761 gctgagaccc cacgcatagc catcgtgacg gacagcaccg ccgacctgcc ggaggatctc
     2821 ctcacgagac tcggcatcac ggtggtgcca ctcaatgtgc atatcggtgg cgagtcgttc
     2881 cgcgaccaga tcgacctgac gaccgacgag ttcatgggtc gattggccga tacacgcgat
     2941 ctcccatcga cttcccagcc ctcggccggg atcttcgagc ggacattccg cgagatcggg
     3001 caagatcacg acgcgatcgt ctgcgtgttg atctcctcgc gcctgagcgg caccgttcag
     3061 tcggcgcagg tcgccgccga cgctgtcggc gatgtggccc gcgttgaggt gatcgactcg
     3121 ctcagcgcgt ccctcggctg cggctttcag gtgcttcacg ctcatcgtct cgccgaccaa
     3181 ggcctcggcg ccaaggagat cgccacccgc ctgcgtagcc agaccatgaa ctaccacgtg
     3241 gtcttctttg tagaaacgct ggatcacctc cggcgaggtg gacgcatcgg caaggccgcg
     3301 tcgctggtcg gatccatgct caagctgaaa ccgctgcttc gcatcgacga ggggcaggtg
     3361 gtcccgttcg agcgcacgcg cacccgcaaa cgcgccatcg aggcactcgt cgagttcgcc
     3421 cagaacatgc cggagatcga gcggatcggc gtgctgcaca acacgacacc ggaggatgcc
     3481 gagcggctca gcgagcgcgt gcagtcgctg gcacgccggg aaagcgaggt gccgatcgcc
     3541 aagttcggcc cggtgcttgg cacccacgtt gggccgggaa cgctcggcct cgccatcgag
     3601 gtggccaaca atgcctgatg cccagcacgt gaccatcgtg accgactcga ccgctgacgt
     3661 gccccccgac atgcgtgagg cgctcgagat cgaggtggtg cccctgcgag tcaactttgg
     3721 acaggagagc ttccgggacg gcatcgggct gaccaccgca caactgatgg agcggatggc
     3781 aaacgccccg agcctgccga cgacctccca gccgacggcg ggtgagttcg cagccgtctt
     3841 ccaggatgcg atcgatgccg gtcgcgaggt cgtctgcatc accatcggcg ccaagctctc
     3901 cggcacgtac caggcagccg tcaccgccgc cgagcaggtc gccctggacc gcatcgaggt
     3961 catcgacggc gcaaccacca ccatgcatat tggctggcca gccgttgccg gggcccgggt
     4021 cgcccggcag ggcgccgacc gggagacggt ctctcggaca atccgtgacg cccgggaacg
     4081 atccaacctc ttcgtggcgc tcaagacgct cgacaacgtc tacaagggcg ggcgcattgg
     4141 caaggcgagc cagctcgtcg gctcggccct cggcatcaag ccgatcgttt ccttccgtga
     4201 tggcacgctc gtgcctattg agcgcgtgcg cacctggaaa aaggccgttc ggcgcatggt
     4261 agagctggtg gtggaggaag gcgacctctc ggatatcatt gtcgtgtata cggacgatcc
     4321 gaccgacgcg gaaaccatcc aggcagagtt gcgggagcac tacccaaacg ccaacgtcat
     4381 cctcgggacg gccggtgcgg tgatcgccac ccacgccggt cccggcgcga tcggcgtcgc
     4441 gaccttgcgt cgcgcctgac tacgcactga cccccgagtc cgacccagac cacatcccac
     4501 cgccagagga gcatgcccat cgtggcgtct cgtacgcatc aggcactatc gccgacatcg
     4561 cgagccgaac agcactacga gcagaccctg cgtgcgctgc gcgtgctgtg gacggcgcgt
     4621 gacagcgcac gcccagagat gcgcgaggct gtggccgcgt atcgccaact gatccctgcc
     4681 gccgcggcga cgcacgagat gcaggacatg ttgcagcgca gcatcgagcg actcgagcgg
     4741 ctaccgatgg acctcgagag ccgtcgaccg atcgtggaga ccttggctgc ggaactgaaa
     4801 gccctccgac cgcgcctcgg gctggtcgag ccgaccgggg aaaagggcgt gctcaacatc
     4861 gccgccgggg agcgccgtgc cggtcgcgac tccccagccc agaacaagcc tcgcacccct
     4921 tcgacgcagg agcgcctgcc gatcgacgcc gcggtcaccg agctgaatgg cgtcggcaag
     4981 aaacaggccg cgatgctcga caagctcggc attcacactg tcggcgacct gctccgcctc
     5041 tccccgcgcc gctatgtcga ctaccgcaat ccgatcaaga tcggccagca cctcgacttc
     5101 accggccata tcctcgtgcg gggcacggtc agcaacatcc gcgagcatcg cacgcgcaag
     5161 gggccgagcc gggtgacgat ggagctgagc gatggaaccg gcgtgctcac cctcaccttc
     5221 ttcagtccat acatcgccag gcaggtgcgc gagggatcgg agatctacgc tgccgggacg
     5281 gtcgagcatg gctacggcaa gctgcagatg gtctcgccgg aatgggacct tgtcggcggc
     5341 ccgaacgtcg ctaccggcca ccttgttccc gtctacccgc ttaccaaggg cctctaccag
     5401 aagaacatgc gccggttcac ccggctcgcc ctcgacgcta cgcggccgaa cctcgtcgac
     5461 tggcttgcgg atatccacga ctacatcgac gacgccgtct ggcagcggat gccctcactg
     5521 gaacaggcgc tcgagcacct gcactaccct cccgatctgc acgccgccac tgcggccgtc
     5581 cgacggatga cgctggagag ccttatcctg ctgcagctcg gcctcgtcaa gcgccgccaa
     5641 gaggcctctg ccacgccggg caccgccttc acattcgacc gcgatcggct cgaccggttc
     5701 atcgacgaac tgcccttcga gctgaccggc gcacagcggc gctcgctcga ccagatcatc
     5761 gcagacatca cccggccgca tgcgatgacg cgcctgctcc agggcgacgt tggctcggga
     5821 aagacagtgg ttgctgccgc catcgcattg gtcgccgccg cgagcggcca ccagaccgcg
     5881 gtgatgacgc cgaccgaact gcttgccgaa cagcactatc ggaatctgac ggcgcagtac
     5941 gcggcccttc acgaggatga gcagccgagc gtcgccctgc tgaccggctc gatcaccgca
     6001 aagacccgac gcgaactgct gactcgcctt gccgaggggg agatcgacgt cctcatcggg
     6061 acacacgcgc tcatccagga tgacgtcgag ttccgcgcgc tcgggctggt cgtgatcgat
     6121 gagcagcatc gcttcggtgt ccggcagcgg agcaccctcg ccgccaaggc gcgcggcaca
     6181 ctgccgcacc tactctcgat gacggcgacg ccgatcccgc ggacactcaa cctcgtgctc
     6241 tacggcgaca tgaacgtctc gatcatcgac gagcgcccgc cgggccgcat cccgatcgag
     6301 acgcggcact acatcggcag cgaacgcgac cgcgcctatg cacaggtgcg cgagcaggtc
     6361 gccatgggcc accagatctt cgtgatctgt ccgctggtcg aggaatcgga caccctggag
     6421 gccaaggcgg cggtcaccga ggctgagcga ttgcagaacg aggtcttccc ggaactgcaa
     6481 gtcgaggtgc tgcacggcag gatgccgagc aagaagaagg atgaggtgat ggccgccttc
     6541 cgcgaccacc agttcgacat tctggtgtcg acgtcggtga tcgaggtcgg tatcgacatc
     6601 ccgaacgcga cggtgatgat gatcgagggg gctgatcgct tcggactcag ccagctccac
     6661 cagttccgcg ggcgcgtcgg gcgcggcggg agcaagagct actgcctgct gctggccgat
     6721 accgtcacgc ttgaaggaca gatacgactc gataccatgg tcgccaccga cgatggcttc
     6781 gagcttgcgc agaaggatct cgagttgcgc ggtcccggcg acttcctcgg cgggactcgg
     6841 cagagcggtc tgcccgattt gggattcctg gtgcaggggt tcgacacacg tttgctcgac
     6901 ctcgcacggt cgacggcgga agcggtgctg gccgagcacc tcgacatcac atggcaggcg
     6961 ttcccgcacc tctaccccca gtaccgggca ttctgggcga ctgccggcac cccggatgag
     7021 gggaagtcgt aggtacgtct ggatcggagc gatcgctcgc ttcctcgctt caaagtagcc
     7081 tgtcagttcg accggagcag ccgcagggtg cggagtggag aaacctctct tgacgacggc
     7141 gccaattctc aaaagttgac atcgtcacgc aagagatttc tcgctaggac tagggcactg
     7201 acgcgccttc tcgaaatgac atcgctggtt gcgcttgcac tcgcggcgac cgtcaccttt
     7261 caaggaccat caccatgcgc gttatctccg gctccgcacg gggccgcaag ctcaagggtc
     7321 caccgtccca cgccacccga ccgatggcgg acaagatcaa gggcgcactc ttcaacgtgc
     7381 tgctctcgct cgacgtccag cccgaccgga tcctcgatct ctacgccggc accggcaaca
     7441 tcggcatcga agcgctctct cgcggcggca gctgggcaga tttcgtcgac aaggggcgcg
     7501 accaggccaa ggtcatccgc cagaatctgg aaaccaccgg cttcagcgac cgggcaaagg
     7561 tccaccagat acccgtcgaa acgttcattg ccaccgcaca cggccagccg tacgacttca
     7621 tcattctcga cccgccctac gccgatccga gcattgtcga gaccgttgag tcgatcggca
     7681 acagccgctt ggtacaatct ggcacaattg tggtaattgg acattggcca cggctcgagg
     7741 tgcccgacac ggtcggacgg ctggaatgtc tcaggaaccg gtgccacggc gacagctgct
     7801 tctcggtcta cgaggtggtg gatgtcagcg gctcggcacc atcgggggag gaacagcaga
     7861 agacaccatg aggctcgccg tctacccggg cagttttgac ccgatcacca atggacacat
     7921 ggacgtcgcc cttcgagctg ccaagctttt cgacgggttg atcgtctcga tctacagcgg
     7981 cacgaccaag gccaacgccc tgttcgaccc cgaggagcgg atcgagctcg ccaagcaggc
     8041 gttcgccgag agcgacgagc cgcaggcgag caggatccgt gtcgagagct tctccggact
     8101 cgcggtggaa tacgcgcggt ccgtcggtgc gacgacgctc gtgcgtggcc tgcgcgcggt
     8161 ctccgacttc gagtacgagt tcaagtacgc gcacatgaac aaccacatcg cgcccgagat
     8221 cgaagtggtc tgcctgatga cctcctcgaa gcattcgttt atcagctcga gcttcatccg
     8281 ggaggttgcg tcgctcggcg ggactgtgga tggtctggta ccgcccaaca tcgccgcggc
     8341 cgtccaccgc cgggtcagcg ccgtgaacca cccgctgccc gcacacggga tggcctgacc
     8401 gcgatcgcaa acgaaatgga ccccgcgccc atggatgaca cgatcgagcg ccccgcaaat
     8461 caggatggag ctgtcgacat gctctacctt gtcgaccgtc tcgaggagct cgtgagcctc
     8521 ggcaagcgcg tgcccttcag cacgcgggtg atggtcgagg aggatgagtt cctcgcgctg
     8581 gtcgaccagc tgcgcgtggc cgtgccgaat gaaatcaagc aggcgcagcg tgtgatcaag
     8641 gagcgggagc ggatcatcgg cgatgcgcag gacgaggcgg cagacattgt cggccgcgcc
     8701 catgaggaag cggaccgtat ggtctcgcag agcgccctgc tggccgaggc ccgacagcgc
     8761 ggcgaggata tcctccgtcg cgccgaggag gagcagcaac ggaccaaggg cgagttggat
     8821 gtctacgtgc ttgagcaggc acagctgatg gaagacgcca tccagcgggc aatcgcgatc
     8881 atgcaggacg ccgccgaaga gagtctcgac agcatcatcg aactcaaggc ggaggttagc
     8941 cgcgcctcag accgtcgttg atccacaccc cccacctggc tatgaccctg ataaggggca
     9001 gtctgtggct gcgccttatg ttgaagcaat ccggcatcac ggcttggcgc gtgacgcacg
     9061 gttggcctta ccgccgtggg cacctaccca gttgccatga cgctcgctac tccgcgaccg
     9121 gattcgtgcc gggcgggagc gcggcctcgc catcccctgc ggccacctcg cgacgccggg
     9181 cgatcgccga cgcaccgaac gcgattgcca ggatcgtgat gatcaccgcg agactcccca
     9241 acgccgggat cttgtagacg tcgatcagca gcatctcgcc accgacgaag accagcacgc
     9301 ccgcgagccc gggcttgagg tacttcaaat cggcgaggta gccagcgagc acgaagtaga
     9361 gcgcccgcaa aaggaatcaa gctcggccgc agatattagg accacatgcc cgaagtttgt
     9421 ctccatagtc gcgatctgtt catgcacttc aatgccacgg tcgtcatcga tgaagaccag
     9481 agttttctga ccgatcggga gacagggaac aaacggatcc gggccgaagt ttccctcgct
     9541 cacccaccca agtgacggtc cagtcgccac catcccgagc ggtgcctttc gagctgcgac
     9601 ctctctcggc gcaccggccc gtgggacctc ccccgcgaac atcccgagca gatctgtatc
     9661 gacgccgtga gccacggcga tccgtgcgcc caagccgagc cggaacggca gcgagactca
     9721 ccgttcctta cctgtgcttc gtcgccatcg cgaccgtccg cccggtatag agcgatgggg
     9781 tctgttctgg cttgcttacc atcgcagctt actgtccctg ccccatggaa aatccgggct
     9841 ccccatcgaa ggtatacagg ttttcggtct tgatgatgcc ccaaccggca ttgttgacac
     9901 gctggagcag cgcgatcacg tgttgatacg ttatggcctg accttcagtg gtgaaccgac
     9961 acctccagtg atcagtgctg aaggtttctg gtagcccctc tggccagact ttcgtgcctc
    10021 ggttgctgat catctccaga ttcagcccgt cgccaccggc atcctggaga gtcaatccca
    10081 gttcagaagg ggctcagtcg tcccagtgca ggaagacgtc gacaccgacc agttccttgt
    10141 gcgccgcttt cctcgtggac ggttccgagc gcactccgat cggctcagag gaggcggcat
    10201 actcgatggg gttgagatgt tccggtcact ggccgagccg ctcgatcacc tctcgcgcga
    10261 attcccgcgt acctacctgc tgcttgctga ctgattcatc gaaaatgtcg tacgtgtgga
    10321 cgccgtcttc gatcgtccgc agccaggcgt tgtgtatgcg ctcggcgacg tcggactgac
    10381 cgacatgcac cagcatcatc actccggcca gcagcaggcc tgaggggttg gcaatatcct
    10441 ggcctgcgcg ccgcggtgcc gagccatgaa tcgcctcgaa catcgcacca tgctcgccga
    10501 cattggcctc tccggccaga cctatcgacc ctgcaatctg ggccgcgaca tccgacagca
    10561 cgtcaccgta caggttcggg gtgacgatca cgtcgaacac ctcgggtgtg tcggccagct
    10621 ttgcagtgcc aatgtcaaca atccagtgct cgtgctcgag ttcaggatac tcctcagcga
    10681 tctcgtcaaa gacgcggtgg aagagcccgt cggtgagctt catgatgttg tctttggtga
    10741 aacaggtcac cttcttccgg ttgttgcggc gcgcgtactc aaaggcgtag cgcacgatct
    10801 tttcgcagcc cggccggctg atcagcttca ggcactgcac cacctcatct gtctggcagt
    10861 gctcaatgcc cgcgtatagg tcctcctcgt tctcccgcac aatcaccacg tccatccccg
    10921 ggtgcctggt cgggacgaac ggagcgtacg cgacgcacgg tcgcacgttg gcgtacaggc
    10981 ccagggactt gcgcacggtc acgttgaggc tctttgaacc ctccaccttg cggtggagtg
    11041 attggggctt tcaggaagag tttcgtgcgt cggagtgact cccatgcgtc ggcgtcgata
    11101 ccagcggaga tccccggcga cagacctgct cgccgatctc gatcgtttca atggcaagcc
    11161 gtgcaccggc agcgctgagc actgacaggg tcgcgtccat gatttccggc ccaatcccat
    11221 cgccatgagc aacagtaacc ggggtaggct cccgcaacgc ttctctatgt gttgcggccc
    11281 cttggcggac ttcggtagca cgtggtgtgg aatcactgga tcgcatgtgt ccctcctgcc
    11341 tggcactgag aaaaccgcgg ctgcgcccaa tccaggcaac actccatggt tcaggcggac
    11401 cgtcaacagt cggtcgttat agccatttct ggccgaatct tcgctgtatc acccgcgtgc
    11461 cgggcggctg ggtcgggtgc tcagacccag tgattggcgg ttggtgctat tgcggcgcca
    11521 ttgtcgggca atgatatcac ccacttacac cgacggctct tcggcagtgc ttgccacgac
    11581 ttgtcagaag ttcatggctc ttgtctggcc aagtctgcat cgtgctgcgt gccttccgga
    11641 atggatgctg gtatgtgcag ttctggcagc aacacatctg cacggcgacg cctcacctca
    11701 cgtgctagac agccggctcg tcacgatgca gcttcaatgc atgcagagta cgaaaacggc
    11761 gggcacgtcc tgcacccgcc gttttcctcg tcgcgagact gccgcccttc tagcttgtcg
    11821 gcgtggcgtc gaccggcgtg ccgccaggca gctccttcgg gaagatggtc acgtgaacga
    11881 atgttgtgcc atcgccctgg tcagcaagct cggcctgacc ggcgaaaccg gaattatcga
    11941 tctcgtgcag atcaatgaag agctcgcctt cgtccggtgt cccggtgatg ttgccacacg
    12001 cgaggtagtt catgatattt tcctcgctct cgtggacgtt gatcgcgaag tcttccgcaa
    12061 gcaactcctc gagcgtggca tcgacctcag tgctgctctg cgccgtgaga ccgaccagac
    12121 cgacaacttc ctccgcatcc ggcgtcgcag ccaagtcgag cggaggcgtc gcatccggct
    12181 cacgaactgc agccgcctcc tgcaaatcgt tcagcggata gacgatatcg ccgaggtcct
    12241 gacaggtacc tgcatggata tgcgccgggt gcagctcaat ttctgcctca aatgggacgg
    12301 caggggtcgc cacgcccggg ggagttgcct gtgcagtcac cgatcccgcc atggtcacga
    12361 ggctcgccaa gagcgtgaac atcgcgacga cgactccggg tacgagtatc cgtttggcca
    12421 tgagaactcc tccttctaaa ccacgttatg ttttccacca ccgggtggtt cgagccggtc
    12481 tgcttcccca cctgcaagtg gcgtgccact ccggtcgctg gacagcgacg tcctaccaca
    12541 agcacaatcc caaaaagcca cgcggtcccg cgtctgccgc cggcgtgtcc ttgggaggga
    12601 acgtgtacgc atgaccgagc accagcaaga tcccgcagcg gccagaccgg ccgcctcctc
    12661 cactccagcg acaccccgcc cctcgcccga cgaggcgctc gaactgctga tggccggcaa
    12721 ccgccggtat gtcgagggcg ccataacgca ggactattcg gcacgccgca ccgcgctgaa
    12781 ggagcatgca ccgatcgccg cgatcctcgg ctgcggcgac gcccgcgtcg gggccgagct
    12841 gatcttcgat cgcggccccg gcgacctgtt catggtgcgc gtagcgggca acttcctgag
    12901 cgactacggg ctggccagcc tggagttttg tgtggagttc ctgcaggtgc cgctgctgat
    12961 ggtgctcgga catacccact gtggcgccgt cacggcagcc gtcgacgtcg ttgagaaacg
    13021 ccaagatctc cctggacggc tcttcgtgct catcgacgcg ctggaaccat cggtactgcg
    13081 cgcgaagatg gacgagccgg cagacctgtt gctggcggcg acagaagaga atgtgcgccg
    13141 gcaggtccgc cggctggaga cgatctcgcc ggtaatcaac gcggcacgcg aggccggtcg
    13201 ggtgaagatc gccggtggca tctatgacat cgacaccggc aaggtgaccc tgctcgacca
    13261 aagctgatat cgcacacctc gccgaacgaa aaagggccgg cgcaaggccg gcccgtgcgt
    13321 gcgacgtggg taattccgct caacccttga atggggattc gaagatctgc tcgtcgatca
    13381 cgctgccctg ggcatcgcgg acggctcggc tgaacgcgaa ggtgttcttg tcgacttgcc
    13441 aggcgctgat ctccaccgcc cggccgtcac cggtcccgta gatgttcacg aacacctgcg
    13501 agtcggtcat gtaggcctcg atgagcagcc agctgtcgga atagttctcg aaccggaaat
    13561 ccggccactc ctccgggttc ggcccatact gcaggatcga cgcatcgaag ccggggcccc
    13621 atccgtcgcg ctcgtagttc ggtagccgga acgtgtgcgg gtaccactcc tcgatccgca
    13681 taccggcatt cagcgccgcc cggaagacgg tcgtggagac ttggcagaca ccgccgccga
    13741 cgtcacgtcc cacccggtcg ccggagacga cgatcgcctc ctggtacccc ttgtcggctg
    13801 tgatctcgcc gatcgcgccg ttgaacgaga aggtgccacc cggcggtacc agcgcgccgt
    13861 cgagcaagct gattgcgacg cggatgttct cgtcccgctc ccacgtgcca ccaacatagt
    13921 tggagtggcc ggtgccgagc agctcggtga tcccgtacga ctccagtttg gtatcgtcga
    13981 tctccggctc aatcagcacg accggaatgt cgaccggctc gtggctgccg aggaagctcg
    14041 tggcgaccac ctccgcgaag gcatccgcct tcatcgcagc gccggtcact gacggctcga
    14101 tggcgacgac accgttgtcc catcccagca cggcgctgac cggcttgcgg ttgaccttgt
    14161 cggcgaactg cgcgcgcagt tcatcggaga ggccatcagt gtcgaccgcc agatgggcgg
    14221 tgggctcgcc cgactccatc acaacctcga tggtgaggta ggatcccacc gtctcgccgg
    14281 tgatctgcca ttcctcgtcg agcactctgg cagtgatcgg cgtgctgatg acctcggtca
    14341 cccgtcgctc gatcggcgcg agtgcctcgg cgttgagtgc gggcaggccc acctgcatcg
    14401 gcaggtcatc cgcgatcggc tcgagcgtcg aaagtgcctg ctggatgtag gcgatagcgg
    14461 cctcgcggtc gaccacgatg ccggcctcac cgggtaccac gaccacctcg ccgtcccgaa
    14521 cttcgatccg ggcgtcgacc ggcaactggc cgacatcgcg ctcgaccgag tcgaaccaca
    14581 agtccagtgc tcgctgatcg agctgtgtct ggagcggcac aacctggtcg gtgccgagaa
    14641 cctcagccac gaacccgagg cgggttgtgg cattgccgtc gcgcccgagt tgttctgctt
    14701 gtgccagaga cgcgtccagg ttcaccgaca cgcccagctc ggccagcgac ggtgtccatg
    14761 tctggccatc gtggctgaag acgacggtgc cgcgctcgag gtcagccgcg cgctccgtga
    14821 gccgcgcctt cgcttcctcg atcgtcaagc caccgactgg cacatcgccc acaacaatcg
    14881 agggatagac acggccggca taggcaccac gaaagacgac gagtccgacc gccaccacca
    14941 gcagcgcgac gagaacggca aggaagattc ggcgaacgag gaccatgcgg aatcgctgct
    15001 caccctgcgg gggagcaatg accggaactg tttccggttg caggtcagga tcgccgcccg
    15061 attcgggcag tacggtgtgt cgcagatggc gaacgcgatc ccgtgaccag cctcggatgg
    15121 ccggcagagg acggtcgatc ccctgcagga cgatgctggc cagggacgcg ttgttgaaag
    15181 aacgcgccat gctctcttct ctcaccccac tacgccgatg gcgataggac atcaccaccg
    15241 ccgtaagctt tcgatgctgt ccctccaggc gcgtggcaag atgactcgga cgcgcgtggt
    15301 gtctccgttg gcgggattca tcatccgttc cgggccctgg ctacacgggt tgggccggct
    15361 cggtagtcca gccattctca cataccgctc gtccgcgtgc cagcctgtca ccagagaacc
    15421 tcgttgccga aaccggtgct gtcggctaga gcttgccgaa cacaatgccc ttgtaggtcc
    15481 agccacagcc ggccacggac tcctggatcg acttcttcat ctcacccgtc gagaaccggt
    15541 agttgtacac ctgccccgga cgcagctcct cggtgaacgc gtaagcggtg ccgaactgta
    15601 ccgattgctt ctgccccatc gtcttgctgg ccgaaaacga gaggtgcggc acgccggcct
    15661 cccagaagac cgtgtactcg tgatccttgg tgcgcacctg cttctctttg tcgtcgagct
    15721 tcatggagat cctgaagacc ttctcgagcc ctgccttggc gaaaatctcg taccactggg
    15781 catcgacgat cttccactcg gcgatcaggt tgacaccctc atcgctgccg tcgacgatct
    15841 ggaatggcgc ggtcggccgg ttgatcgcca gaatccgctc gcgcacctcg tccgccgagc
    15901 aggccgcgac gcccggttcc ggccgcttcg tcgacgtgag ccagtccagc acacccacga
    15961 tccgcctccc cgcaagacga cattcacgtt gtggtcccac gagccatcat accagccaat
    16021 ccggatgcgg acctatgaag acggcaacac ctcgcccggt tcgacgaccg cttccgatgc
    16081 ggtcggcaca tgcaggtcgg tctcctgccc cgtctcggcg gatcggtaga ttccgtcgag
    16141 cacccgcagg atgtcgagcc cgtgcgtgat cgtcgccccg ggctgccggt gatcgcggac
    16201 gctgtcgacg aagtccgcga tctcagcccg gaacgactcg tagagctgga atcctggttg
    16261 ttgggcggcg ggcgagtgga actccggcgt gctcaccatc gtcgttcgcg ttgcgcccgt
    16321 gccggaggtc gtgacgatct tcagatcctc ggcggggccg cgctgcatct tcgcaccgcc
    16381 attggtgccg aagagctgac tgtagaccag gttgccctcc tcaatgtggg agagccacga
    16441 gacatcgagc atgaccgtcg cgccgttgtc gagcttgatg aacgcggtgg cgaggtcctc
    16501 gacgtcgacc tccgcgccgg tgctgtcccg catgaactcg ttgaaggtgt agtagacagc
    16561 cccgctcacg ctcaccgggc gggggttgcc catgaaccag agagacagat cgagcatgtg
    16621 cacgccgagg tcgatcagcg ggccgccacc ggaccgctcc cgctgcgtga accagcttct
    16681 atcccggatg aagaagtgcg acgcgccgcg cagccatccg gtcttcgcgt agtagatgtc
    16741 acccaactca cccgcgtcga ggtgctgctt gagcacgaca ctgtcgggcc ggtagcggtt
    16801 gttgagcgca atcatgaaga cctgatccgg atgggcggcg accttctcgg cgagccgctc
    16861 gccctcggct agggtacgcg cgaggggctt ctcgagcagc acatgcttgc ccgcgtcgag
    16921 cgcatcgatc gccatggagg cgtgcaggaa gttcggcgtg cagatgctga ccgcatcgag
    16981 atggtcgctg gcgagcagct cctgatagtc gccgaagatc tccgccccct ggacgttgta
    17041 ctccgccttg acctgctcgg cggcggctgt gttgagatcc gagagtgcga cgatctgcac
    17101 atcgggatgc ttctggtacc cctcgatgtg ccgctgtcct ggccaaccga cgccgatgat
    17161 gccaacgcga agtgtgttca tgaagccccc ctctcgaatg caagagcacg tgggcgttgc
    17221 cgggtgcgca cgtcaacatg ctggatagca tagcccctcg gcacggccag gtcgccaccg
    17281 cccagaacct cgtcctgctc gaacgctcct gccgctcgtt ggtcggctca ttgtgtatgg
    17341 cacaggtatt gcagtctcac gagaccggct gacggcggtc tccgggcagg ccccagacga
    17401 acgccggcgg tcgcgtcaga tcgatagagc ggatgttgag atcaacgcag cggaagggcg
    17461 aacgatgatg ggacgccatg gcagcacagc ttccccgtcc ccttgggtag catcggggct
    17521 ctcggtgctg gtcgaggggt ttggacaggc atacaaccgg cagccggtga aagcggccgt
    17581 gttcctgatc acgggactga ccctcagcac cggcagtgga ctgaatacgt ggctcgccca
    17641 gaacatcctc ggcttgaagg gcacgcgact cggtcctgag cgcgtgcgcc cgggcctgct
    17701 cggtctctgg ttcgcgacgt tcgtgctgaa cctgttcgac gcctggagaa cggcgcggcg
    17761 gtagcgccag cagccagact cgtgatcgac gcccgcgccc ggtcgcggct gagtgccgaa
    17821 ccgagcgcgg atgaacggct gtgtcactcg aagagaagat cgagcgggtg tgaggcgtag
    17881 gggcgaccca gcatgcggcg ctggtcgctg tcggcgagcg cctcgcgcac cttgtccggg
    17941 tccatccact cagggtacgg aggcaacgcc ggggcttctc gcttgggcag cgacggggtg
    18001 agggcgcgga tcgccgcctc agccgtcagg tagccgtggt tctccagcac cttctgctcg
    18061 gcctcgctga acgcgtcgag gtcggtccga atctgggcaa tgactgtctc gaccaggtcc
    18121 cgcgggtaca cggttgggga gccgatctcc tgcaggtccc acttcggctc gtcgatgccc
    18181 cagatggtgc cgtcgaacag tccggcgcgg aagctggcga agagccagcg cttctgcacc
    18241 acccgcacgc gcgcgttcgc gatcgccggg tagcggcgga gccgccagag ccaagagttg
    18301 cgccagcggg tatcgaaggt cccgccaccg tcggagacga ggatggtggc gtggtcctcc
    18361 cagaccgcct cgaccccggt gttgtcgtag gtgccgccgt ccgtcagatg cccccggtca
    18421 accttgcgct tgggtgggcg ggcggtgccg gcctggcggc cgtccggtcc gggactcatg
    18481 gccaccgcca tcggcgcgaa gatcggcgga aagcaggagg aggcggcgac cgccctcgcc
    18541 actggccact tcggatatgg cgccagctcg gtatcgaacc gaccggcgcc gcgcttctcg
    18601 aacacccaga agcggccgta gaccatatcg gttgcacaga agatgtagcg cgggcgatcc
    18661 ggcaggtccc cgagccgtgc cgaggtaatc tgccgctggt agcgccgtgc gagcgcccga
    18721 atgccggctt ccggacgcgt ccagttccac ggcaggaacc gccggaggac cggcccggtg
    18781 cggaggttct ggctcgtgat gtcccggaac ggctgggcaa cctgcccgtc ccagtcgtcc
    18841 acgacgtcgc ccttggcggg ccactcgggg atgcgctcga caaggtgcgc ggcgaggatg
    18901 ctgccgcccg atacggcgga gatggtggcg acgtcgccga ggatgccgag ctcgttgagc
    18961 cggcgcaggg caccgaggtg gtagagcgcg gcgcggaacc cgccgcccga gaggcagagg
    19021 gcgatcccct cacgctcgtc gcgattccct gcccgcaaaa tggacgagga atcctccatc
    19081 gcctcgcgcc gattcttctc tgacactcga ccatcctcct ctagtatgcc ctgcattgtc
    19141 ctgcaatcgc tgaaaagcgc aagcgcgatg ccactgcgtc cgtggccgga ggcccggcct
    19201 tccgctacca tgcggcatcg gccagccacc ccgtgaggta cgccatgcag cgcgatgtct
    19261 ccttaccagc ctacgtcccg gagcagatcg cgtgctatgg gggcgccgat ctcctcacgc
    19321 aggaggtctg ccggttcgac cccgcgctgg ccgtggaacg actccagccc cagcaggaac
    19381 tgctggcgat cgacatcggc ggcgacaaga tccgcagcgc gatctaccgc gccggcggcg
    19441 gaacgctcac gaaggtgagc gaagagatgt tccggtcgag cggcggagct ctaccgccgg
    19501 gcgctggccg agtaccgccg ccacccgctg gcgctgccgc ccgatcgggc ctactccccg
    19561 gccagcatcg gcaaggctta tttgaaagcg atgggggtca gggtgccgga actcgtgccc
    19621 gatccgaagc tggagctctc cgtcgacgcg gtgttcggct acgccatggc ggcctactac
    19681 ggcgggcggg ccgagtgccg ggtgcgcgag gtgccggtcc cggtggccta ctgcgatttc
    19741 cgcagcatgt acccgagcgt caacacgctg atggggctgt ggcgcttcct caccgcccag
    19801 cgcctcgagg tcgtcgacgc caccgcctgg gctcagaact tcctcgcccg caccacggcc
    19861 gacgattgct ttcgaagggc gacgtgggag cagctggcgg cgctcgtcga ggtcgagccg
    19921 gacggcgagc tgctgcccgt gcgggccccc tacagcgccg agcaggcggg ctacaccatc
    19981 ggggtcaacc cgctccgcgc aacggagcag gtggaaacga tctggtacac gctcgccgac
    20041 tgcctcgcgg cgaaaatcgc caccggccgc gcgccgcgag tccggcgggc gctgcggttc
    20101 gtaccgaaag ggcggcagcg tggtctgcac cccgtggatc tgcgcgggca ggtcaccgtt
    20161 gacc
//
LOCUS       DASYWL010000049         5756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_227264, whole genome shotgun sequence.
ACCESSION   DASYWL010000049 DASYWL010000000
VERSION     DASYWL010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5756
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227264"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="VGR22_01710"
     CDS             complement(<1..864)
                     /locus_tag="VGR22_01710"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127315.1"
                     /translation="MCGIFGYVGEPVKVGPVVLEALRSMEYRGYDSWGVAFTVGDQPG
                     EVIKAVGRVEVGAAADSEATAAIGHTRWATHGEVCERNAHPHLGCGGRIAVVHNGIIE
                     NVDGLRGELDDLLLASDTDSEIMAHLVSRNVGLGQSLREVVQIAFSSIEGNNAFVVLD
                     RATGEVVVVTRRLPIRLSRHDRGVVLASDPAALSGMVETAVALPDDTAVSLGTPTRVA
                     EGIDRLIAGGRRIAVPDRGERIKGGPGASMRAEITEQPSVLRRIAADTEPVQAAAELA
                     RQADRVVFTGCG"
     gene            1361..1747
                     /locus_tag="VGR22_01715"
     CDS             1361..1747
                     /locus_tag="VGR22_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEV2127316.1"
                     /translation="MASKAIVESNKAPSAIGPYSQAIRVGDMLYTSGVVPLDPETMEI
                     VGTTIEEQTERVMESMKALLEDSGTDLGKVVKTTCFLDDLGNFAAFNEVYARYFGDSK
                     PARSTVEVAALPKAAMVEIEAIALLD"
     gene            1819..1892
                     /locus_tag="VGR22_01720"
     tRNA            1819..1892
                     /locus_tag="VGR22_01720"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1853..1855,aa:Val,seq:cac)
     gene            2242..2970
                     /gene="extP"
                     /locus_tag="VGR22_01725"
     CDS             2242..2970
                     /gene="extP"
                     /locus_tag="VGR22_01725"
                     /inference="COORDINATES: protein motif:HMM:NF040969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenite/tellurite reduction operon b-type
                     cytochrome ExtP"
                     /protein_id="HEV2127317.1"
                     /translation="MRREQTLADRITGSALWRSVVRHGYPDTQRNQALIIASNVFLHI
                     HPVKIKRYATKVTYTFCLGGLSFFMFLVLTMTGVLLMFYYIPSADQAYQSMQDIEASV
                     SFGQLMRNMHRWSAQGMVIAVFLHMGRVFYTGSYKPPREFNWVVGVMLLVVTFLLSFT
                     GYLLPWDQLAYWAITVGAEIGGSAPIVGPQVNRILLGDFQIGQSTLIRFYTLHVIFLP
                     LVAAFLMAVHFWRIRKDGGISGPL"
     gene            3017..3808
                     /locus_tag="VGR22_01730"
     CDS             3017..3808
                     /locus_tag="VGR22_01730"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127318.1"
                     /translation="MAELANRPPGSAAQQIDEAKRRKLLELVRGRALARPTDLAEDEV
                     MVWPSLVVVEAVSAVVFLGILTALSVLVNAPLLERANPNQTPNPSKAPWYFLNLQEML
                     LHMDAGLAGVIIPTFALLGIAAIPYVDRSPLGVGILGTSRKGRKIIAFTAVFTTVVLT
                     GMVLLDEALGRGTQFGLGNLLQEELGAPDWVGSIVLSSLIILAAIAVLVALVQGIFRP
                     TRRELIIALFTGFFVTYWVLAFFGTSFRGEGQELTWPWNLPLEHH"
     gene            3885..4478
                     /locus_tag="VGR22_01735"
     CDS             3885..4478
                     /locus_tag="VGR22_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEV2127319.1"
                     /translation="MTPLVRTLAGIAGGWLVAKVLGLLKLLQATHGATPAKKLSSRRS
                     FTRNAALGAVGIVTAQIAGGFVWLLWPNKTGAFGGEINVSANNVPEVGGEPFRYSQGQ
                     FFVVHTENGVLALWWKCPHLGCTVPWISAENRFICPCHGSVYDYNGERISGPAPYGLD
                     IMPVTVLDDGAIVVDTNPSTIIVGSADRSELAVEYTA"
     gene            4538..5494
                     /locus_tag="VGR22_01740"
     CDS             4538..5494
                     /locus_tag="VGR22_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEV2127320.1"
                     /translation="MGAVQKLATVVIIGLVALATVLTVYIANEPGRRDAEAEEQQDIS
                     IVRGTELYITFCLQCHGPAGLGAAGGEENPRIGPPLNNEFYQSDDPVQLQRAESLIRY
                     RVLNGAPSDPRIEDKVMPSFRNDLSDAQLNTIVTLILHGDWDYVYNRSVLQTGQQVAQ
                     DACRHEGGEAEVCDHIEEGSVDAPPAYPTAPPPEVADPGESAEGANLGEDAPEEEGVA
                     AEAEAETGVVTLEAADPYEWSETNFTVQAGDTFITVNTGSLQHDFTVDELGIHEDLPT
                     DGTNVTITIPEDAEPGEYEFYCSVPGHREGGMYGILVVEPAS"
     gene            5507..5713
                     /locus_tag="VGR22_01745"
     CDS             5507..5713
                     /locus_tag="VGR22_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127321.1"
                     /translation="MIISNLALVFGTLAETVELWLAIIMMLSGIYLTHLIQRWADSAG
                     EHPSAANIGLSGSAERREPKSMRE"
BASE COUNT         1047 a         1915 c         1738 g         1056 t
ORIGIN      
        1 gccacacccg gtgaagacca cccggtcagc ctggcgagcc agctccgccg ccgcctgcac
       61 cggctcggta tcggccgcga tccgtctcag caccgagggc tgctcggtga tctcagcgcg
      121 catcgacgcg ccaggtccac ccttgatccg ctcgccgcga tccggcacgg cgatccgccg
      181 ccctccggcg atgagccgat cgatcccctc ggcgacacgc gtcggcgtgc cgaggctgac
      241 cgccgtgtcg tccggcagcg caaccgccgt ctcgaccatg ccggagagcg cagccgggtc
      301 cgacgccagc accacaccgc ggtcgtgccg gctgaggcga atcggcaggc ggcgtgtgac
      361 aacgacgacc tcgcccgtcg cccgatcgag caccacaaac gcgttgttcc cttcaatgga
      421 agagaaggcg atctgcacca cctcgcggag ggactggccc agcccgacgt tgcgcgacac
      481 gagatgggcc atgatctccg aatcggtatc ggacgcgagc aggaggtcgt ccagctcgcc
      541 ccggagaccg tcgacgttct cgatgatgcc gttgtggacc accgcgattc ggccgccaca
      601 gccgaggtgg ggatgcgcgt tgcgctcgca cacctcgccg tgggtcgccc agcgggtgtg
      661 cccgatcgca gccgtcgcct cggaatccgc ggcggcgccg acttcgacgc gaccgaccgc
      721 cttgatgacc tctccgggct ggtcgcccac ggtgaaggcc acaccccacg agtcgtaccc
      781 gcggtactcc atcgagcgaa gcgcctcgag caccacggga ccgaccttca ccggctcacc
      841 cacataccca aagattccgc acacgctcca cctcccatat gacaggccgt ccccgccagc
      901 atgccggggc acggctccgc ctggtgcctg gtcgcaaggc actgcacggc tcacccgggc
      961 acgccgggtg gctcgaacaa aaacgcattg tcgggtgacg tggagggggt ggcctcgtaa
     1021 tatgggccaa cgacacgccg agcggagcat tgttcgcgcg tcaccgcgcg gctttgtctc
     1081 gctcgtgacg actgtgcctt gccgggaagc catccgccga atggttcgat cggcttccgc
     1141 ctcgtcaact cggtctcccg ccgagtcctg gcgctggaat gcgaatacgg tccccatcac
     1201 cacgtcgtac ggacatcgta gccccctttc gggctggcgt ccagtggtcc tgcaggacgc
     1261 ggggcatggc cctgcacgtt cggcttggta ccatgctcag cggtacgatc ggcgtatcgg
     1321 gcggcccgcg ggccgagcag gagaagtgac aggagatctc atggcgagca aagcgatcgt
     1381 ggaatcgaac aaagccccat cggcgatcgg cccctactcc caggcgatcc gcgtcgggga
     1441 tatgctctac acctccggcg tggtgcccct cgatcccgag acgatggaga tcgtgggcac
     1501 gaccatcgag gagcagaccg agcgcgtgat ggagagcatg aaggcactgc tcgaggattc
     1561 gggcacggac ctcggcaagg tggtcaagac cacctgcttc ctggacgacc tcgggaactt
     1621 cgctgccttc aacgaggtct acgcccgtta tttcggtgat tcgaagccgg cccgctccac
     1681 ggtcgaggtc gccgcgctgc cgaaggcggc gatggtggaa atcgaggcga tcgccctcct
     1741 cgactgaggt gacccggtga cacacgcggg gttggcgact cgccgacccc gcgtgtatac
     1801 tcgcttcgct gcagatacgg gcgcgtagct cagctggcca gagcgcacgg ttcacatccg
     1861 tgaggtcaca ggttcaagtc ctgtcgcgcc caccctcaac cccgcatccc gagaatcgga
     1921 tgcgggtttg ctgtacctaa cacccgaccg ccgtgcggct tggcacaccc gacatgcgcg
     1981 cctatttcct aggtactaga cggagcgagg atatggtcag ggatgccgat ctttcatcga
     2041 tatcgcgccg gatcaccctt gaccccacct aaccagccgg aacacaatac gcctacagcg
     2101 ctgccgccgg ctggttgact ggcggtacac gatcgagatc attaccctac cttcccggag
     2161 gatacctcct cacgcggaag gatgattcat gagcgacgtg ttttggtctg tcagccggag
     2221 aggattcgga ggaggagtgg catgcggcgt gagcagacgc tggccgatcg cataaccggc
     2281 tcagcgttgt ggcgttcggt ggtccgacac ggctatccgg atacacagcg caaccaggcc
     2341 ttgatcatcg ccagcaatgt tttcctgcac attcacccgg tcaagatcaa gcgctacgcg
     2401 accaaggtga cctacacctt ctgcctgggc ggtctctcgt tcttcatgtt cctggtcctg
     2461 accatgaccg gcgttctgct gatgttctat tacattccct cggcagatca ggcataccag
     2521 tcgatgcagg acatcgaggc cagcgtctcc ttcggacagt tgatgcgcaa catgcatcgg
     2581 tggtccgcac aaggtatggt catcgccgtc tttctccaca tgggccgcgt cttctacacc
     2641 ggttcctaca agcccccccg cgagttcaac tgggtcgttg gcgtgatgct gctcgtcgta
     2701 accttcctgc tctccttcac cggctacctc ctcccatggg accagctcgc ctactgggcg
     2761 atcacggtgg gcgccgagat cggcggatcg gccccgatcg tcggtccgca ggtcaaccgc
     2821 atccttctgg gcgacttcca gatcggccaa agtacgctga ttcgcttcta cacgcttcac
     2881 gtgatcttcc tgccactggt cgcggcgttc ctgatggccg tgcacttctg gcgcatccgc
     2941 aaggacggcg gtatctccgg gccgctgtag gccggtcatg ccgcttcgct tcctcgcgta
     3001 gagaaggacc caacgcatgg cagaactcgc gaaccgccca cccggctccg ccgctcagca
     3061 gatcgacgag gcaaagcgcc gcaagctcct tgaactggtg cgtggtcgtg ccctggcccg
     3121 tccgaccgac cttgctgaag atgaggtgat ggtctggccg tccctggtcg tcgtcgaagc
     3181 cgtgtcagca gttgtttttc tcggaatcct gacggccctc tcggtcctcg tgaacgcccc
     3241 gctgctcgag cgggccaatc cgaaccaaac gccgaaccca tccaaggcgc catggtattt
     3301 cctgaacctt caggagatgc tgctgcacat ggacgccggt ctcgccggcg tgatcatccc
     3361 gacgtttgcc cttctgggca ttgctgccat cccatacgtg gaccgcagcc cgctcggcgt
     3421 cggcattctc ggtacctcca ggaagggccg gaagatcatt gccttcaccg cggtgtttac
     3481 caccgtcgtg ctcaccggta tggtccttct tgatgaagcg ctcggtcgtg gcacccagtt
     3541 cggtctcggc aacctgctcc aagaggagct aggcgcaccg gattgggttg gcagcatcgt
     3601 tctctcgagt ctgatcatcc tcgctgctat cgctgtgctc gtggcgctcg tccagggcat
     3661 tttccgccca acgaggcgcg agctcatcat tgcactcttt accggctttt tcgtgaccta
     3721 ttgggtgctc gcattcttcg gcacgtcgtt ccgaggcgaa ggccaggagc tcacctggcc
     3781 ctggaacctc ccgctcgaac atcactagcc agcggcactc cgccattcaa caagcatgtc
     3841 gggcggtgga gacatcgccc agcctaggca aggaacactg acgtatgact ccacttgtac
     3901 gaacgctcgc gggcatcgcg ggcgggtggc tggtcgccaa ggtccttggc ctcctgaagc
     3961 tcctccaagc cactcacggc gccaccccgg cgaagaagct ctcgtcccgt cgctccttca
     4021 cccgcaatgc cgcccttggt gcggtcggca tcgtgaccgc ccaaatcgcc ggcggcttcg
     4081 tctggctgct ctggccgaac aagaccggag cattcggcgg tgagatcaac gtcagcgcca
     4141 acaatgtgcc ggaggttggc ggtgagccat ttcgctactc gcagggccag ttcttcgtgg
     4201 tgcatacgga aaacggcgtc ttggcgctct ggtggaaatg tccacacctc ggatgcaccg
     4261 tcccgtggat cagcgccgag aaccgcttca tctgcccgtg ccacggctcc gtctacgact
     4321 acaacggcga gcgcatctcc ggtccggctc cgtacgggtt ggacatcatg ccggtgaccg
     4381 tacttgacga cggcgccatc gtcgtcgaca cgaacccctc gaccatcatt gtcggaagtg
     4441 cggaccggtc cgaactcgcc gttgaataca cggcctagga acctgcttcg cgggtcgctg
     4501 ctcgcgggcc acgctagagg atgaggagaa gcgaaacgtg ggagctgtcc agaagctcgc
     4561 gaccgtggtg atcatcggcc ttgtcgcact cgcaactgtc ctgaccgtct acatcgcgaa
     4621 tgagccgggc cgtcgcgatg ccgaagccga ggagcagcag gacatatcga tcgtccgggg
     4681 taccgagctg tacatcacgt tctgcctgca gtgccatgga ccagccggtc tgggtgccgc
     4741 cggtggcgag gagaacccac gcatcggccc gccactgaac aacgagttct accagtcaga
     4801 tgacccagtc cagttgcagc gggccgaaag cctcatccga taccgtgtcc tcaatggcgc
     4861 cccctcggac ccgcgtatcg aggacaaggt gatgccgtcg ttccgcaacg acctcagcga
     4921 cgcgcagctc aataccatcg tcacgctgat cctccacggc gactgggact acgtctacaa
     4981 tcggtccgtc cttcagactg gccagcaggt tgcacaggat gcctgccggc atgaaggcgg
     5041 tgaagcagaa gtgtgcgatc acattgagga gggctccgtc gacgcgccgc cggcctatcc
     5101 gaccgcgccc ccgccggaag tcgctgatcc aggtgaaagc gccgaaggcg cgaatctggg
     5161 cgaggacgct cctgaggaag aaggggtagc ggctgaggct gaggctgaga ctggcgtcgt
     5221 cacgttggaa gccgccgatc cgtacgaatg gagtgaaacc aatttcaccg tccaggcagg
     5281 tgacacgttc atcacagtaa ataccggatc gctgcaacac gatttcacgg tcgatgaact
     5341 cggtatccac gaggatctgc cgacagacgg taccaacgta acgatcacga ttccggagga
     5401 tgccgagccc ggggaatacg agttctactg ctccgtaccc gggcaccggg aaggcgggat
     5461 gtacggcata ctggtagtcg agccagcgtc gtaggagatc atcagaatga tcatatcgaa
     5521 ccttgccttg gtattcggca cactggccga gacggttgag ctttggctcg cgatcatcat
     5581 gatgctgagc ggcatctacc tgacccacct catccagagg tgggccgaca gcgccggtga
     5641 gcatccgtct gccgccaaca ttggcttgag cggcagcgcg gagcggcgcg aaccgaagtc
     5701 catgcgcgag tagcaggacc ttccggcaac tcgcgtgacg gcccggatgc atgatc
//
LOCUS       DASYWL010000050         3445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_20845, whole genome shotgun sequence.
ACCESSION   DASYWL010000050 DASYWL010000000
VERSION     DASYWL010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20845"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VGR22_01750"
     CDS             <1..366
                     /locus_tag="VGR22_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127322.1"
                     /translation="IGAGYISAIYLENFTTRFANLRVVGIADLVPEHARRQAERFGVA
                     AMASFPLAKMRLRFNRVLFLLFIAGAMIPVHVTLIPIYTLTNRIGIYDSLLGTPGAVR
                     CVSPAAGDPDPHRVHAPAT"
     gene            284..700
                     /locus_tag="VGR22_01755"
     CDS             284..700
                     /locus_tag="VGR22_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV2127323.1"
                     /translation="MALLGPYVAFHLPLAILILTEFMRQLPKELEEAARIDGASHIEI
                     FMRIIMPLTTPALSTVAIYTFIYVWNEFVFALVLLSSRSNLTLPLGLMQFSGEYSVNV
                     PGLMSAVTLASLPVILFYLVMQERVVSGMVAGAIKG"
     gene            complement(1009..2529)
                     /gene="xylB"
                     /locus_tag="VGR22_01760"
     CDS             complement(1009..2529)
                     /gene="xylB"
                     /locus_tag="VGR22_01760"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HEV2127324.1"
                     /translation="MPYMLGLDIGTSGAKAVLVGEDGSVAGVGNASYPLSHPRPLWSE
                     QDPDDWWAGAKAALREAIQGDGVAPDEIVALGLTGQMHGAVFLDETDTPIRPAMLWND
                     GRTDAQCDEIERRVGLERLLAITGNPALEGFQAPKILWVREHEPEAYVRVRRVLLPKD
                     YIRLLLTGDAATDASDASGTLLLDLAAREWSDKLLSALDIPRAWLPTVHESPELTGKL
                     REQVAAELGLPAGLPVAAGGGDNAAAAVGTGIVREGFVSSSVGTSGVIFAHTDAFLPD
                     PEGRLHAFCHAIPDKYHLMGVTLAAGGSFSWWRDLLDPEMSFGDLSAEAASAPPGADG
                     LLFLPYLSGERTPHRDPLARGAFVGLTARHTRAHMTRAVMEGVIFSLRESLALMRNLG
                     VQIDHVRATGGGARDPLWRELQADIYRTPVRRMRVEEGPALGAALLAGVGAGVFVDVM
                     AASETIALHAEAQAPDPERADRYDGLFRLYRSLYPTAADIMHELNTFASSADSAGT"
     gene            complement(2783..>3445)
                     /locus_tag="VGR22_01765"
     CDS             complement(2783..>3445)
                     /locus_tag="VGR22_01765"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEV2127325.1"
                     /translation="LSQGYVLKFAIEPKPNEPRGDIYLPTIGHALAFIETLDHPEMVG
                     LNPEFAHEVMAGLNFVHAVGQALEAGKLFHIDLNDQQPSRYDQDFRFGSENPKRAFFL
                     VKLLEDAGYDGPRHFDAHAYRTEDEEGVWAFARGCMRTYLILQEKVQRFNKDREVQAL
                     LAELSKQDKSYDGPRAGEGYSADHGAQLKEQTFDVAVLAERGRRYEELDQLVMEVLLG
                     VR"
BASE COUNT          548 a         1118 c         1128 g          651 t
ORIGIN      
        1 atcggggcgg gctacatctc cgccatctac ctggagaact tcacgacgcg cttcgccaac
       61 ctgcgggtcg tcggcatcgc cgacctggtg ccggaacacg cgcggcgcca ggccgagcgc
      121 ttcggcgtcg ccgcgatggc cagcttcccc ttggcgaaga tgcgactccg attcaaccgc
      181 gtactctttc tgctcttcat cgctggcgcg atgattccgg tgcatgtgac gctgatcccc
      241 atctacacgc tgaccaatcg cattggtatc tacgattcgt tgcttggcac tcctggggcc
      301 gtacgttgcg tttcacctgc cgctggcgat cctgatcctc accgagttca tgcgccagct
      361 acctaaggaa ctcgaggagg cggcgcggat agacggagcg agccacatcg agatcttcat
      421 gcggatcatc atgccgctga ccacgccggc gctgagcact gtcgccatct acacgttcat
      481 ctacgtctgg aacgagttcg tcttcgcgct cgttctcctg tcgtctcgct cgaatctgac
      541 gcttcccctg ggcctcatgc agttctccgg cgagtacagc gtcaacgtgc caggtctcat
      601 gtccgccgtc acgctggcca gcctgccggt gatcctgttc tatctcgtga tgcaggaacg
      661 ggtcgtatca gggatggtcg caggcgcgat caagggctga ctcggttacg ctcgattcct
      721 ttgccggccc gatttttcgt tttgataccg tggcccgttc ctccggctcc gagtcacctg
      781 ccgataattt tctgcagcgc aaacttgccg agttgcacct ccgctgatgg caggacgaag
      841 ctggtacctc gctgacttgt gcgcgtgttt tcccgtcaaa gaggcgttac tggacaggta
      901 gtgttcggaa tgggccgcag ggattcgacg cacatagtgg cctgcggaac gtggggtgtg
      961 caatccgcca aggcaccggt ccttctgagg tgcagcaacc cggcgcccct atgtgcctgc
     1021 agaatcagca cttgaggcga aggtgttgag ctcgtgcatg atgtcggcgg cggtcgggta
     1081 gaggctgcga tagagccgaa acagtccgtc gtagcgatcg gcccgctctg ggtcgggcgc
     1141 ctgtgcctcg gcgtgcaggg caatcgtctc gctcgcggcc atgacatcca cgaagacgcc
     1201 cgcaccgacg ccggcgagga gggcggcacc tagcgctggt ccttcctcga cccgcatccg
     1261 gcgaactggt gtgcggtaaa tatccgcctg cagctcccgc caaagcgggt cgcgcgcgcc
     1321 gccgccagtg gctcgcacgt ggtcgatctg cacgccgaga ttccgcatca gcgccagcga
     1381 ctcacgcagg ctgaaaatga ctccctccat cacggcgcga gtcatgtgcg ctctggtgtg
     1441 gcgggcggtc agccctacga aggctcctcg cgcgagcggg tcgcggtgcg gggtgcgctc
     1501 gccgctcagg tagggcagga agagcagccc gtcggccccc ggtggggcac tcgccgcctc
     1561 ggcggagagg tcgccaaagc tcatctcagg atccagcaag tcccgccacc aagagaacga
     1621 cccgcctgcg gcaagggtca cacccatcag atgatacttg tcggggatcg catggcagaa
     1681 ggcgtggagc cgtccctctg ggtcgggcag gaaggcgtcg gtgtgagcga agatgacacc
     1741 gctcgtgccg accgaggagc tgacgaagcc ctcgcgcacg atcccggtac cgacagcggc
     1801 ggcggcgttg tcgccaccac cggcggcgac cggcagtccg gcgggcaggc cgagttcagc
     1861 ggccacctgc tcgcgcagct tcccggtcag ctccggactc tcgtgcacag tgggcagcca
     1921 ggcgcgtggg atgtcgagcg cggacaggag cttgtcggac cattcgcggg cggcgaggtc
     1981 gagcagcagc gtaccgctgg catcagaggc atcagtggcg gcatcgccgg tgagtaacag
     2041 gcggatgtag tccttcggca gcagcacgcg ccggacgcgc acatacgcct ccggctcgtg
     2101 ctctcggacc cagaggattt tcggcgcctg gaagccctcc agtgccgggt tgccggtgat
     2161 tgccagcagc cgctccagcc cgacgcgacg ctcgatctcg tcgcactgag cgtcggtgcg
     2221 gccgtcgttc caaagcattg ccgggcggat cggcgtgtcg gtctcgtcga ggaagacggc
     2281 gccgtgcatc tgaccggtca ggccgagcgc gacgatctcg tcaggggcca cgccgtctcc
     2341 ctgaatggct tccctgagtg ccgccttcgc accagcccac cagtcgtcgg gatcctgctc
     2401 actccagagt gggcgagggt gggagagcgg gtagctggcg ttgccgactc cggcgacgga
     2461 gccgtcctcc cccaccagca cagccttcgc gccgctcgtg ccaatgtcga gtccgagcat
     2521 gtatggcatt gttggtctcc atggtgctgg cgttctggat gcccgatgat gacgaggttc
     2581 aaggcatcac gccagtgtca tttttcgacc cggctgcgac gactgtgcgc ctgggcggct
     2641 cgagatgaca ttgagccggt ctctcacagg caacggctgg ctgcctcggt tgttgggagg
     2701 gcgaatacaa ggtccgcccc tacgaatagg gcggcggtgc cgtattcgtt ccgaatcctc
     2761 cccgcacgat tcccttgccg tcctatcgca cgccgagcag cacctccatc acaagctggt
     2821 cgagctcctc gtagcggcgg ccgcgttcgg cgagcacggc gacgtcgaag gtctgctcct
     2881 tgagttgggc gccgtggtcg gccgagtacc cttccccggc ccgcggcccg tcgtagctct
     2941 tgtcctgctt gctcagctcg gcaaggagcg cctggacctc gcggtccttg ttgaagcgct
     3001 gcaccttctc ttggaggatc aggtacgtgc gcatgcagcc gcgcgcgaac gcccagactc
     3061 cttcctcgtc ttcggtacgg tacgcgtggg cgtcgaagtg gcgcggaccg tcgtagccgg
     3121 cgtcctcgag cagcttgact aggaaaaagg cgcgcttggg gttctccgag ccgaagcgga
     3181 agtcctgatc gtagcggctc ggctgctggt cattgaggtc gatgtggaag agcttgccgg
     3241 cctccagcgc ctgcccgacc gcatggacga agttgagccc cgccatcacc tcgtgggcga
     3301 actccgggtt gagacccacc atctccgggt gatccagggt ctcgatgaag gcgagcgcgt
     3361 ggccgatggt cggcaggtag atgtcgcccc ggggctcgtt cggcttgggc tcgatggcga
     3421 acttcaggac gtagccctgc gagag
//
LOCUS       DASYWL010000051         2840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_659081, whole genome shotgun sequence.
ACCESSION   DASYWL010000051 DASYWL010000000
VERSION     DASYWL010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659081"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..919)
                     /locus_tag="VGR22_01770"
     CDS             complement(98..919)
                     /locus_tag="VGR22_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2127326.1"
                     /translation="MSDNVIWPREGELEQGWFAVDSPAEGVWRVQEPLHEENVKSFLV
                     LGVERAALIDTGMGVGDILAVVERITTLPVVVINSHAHWDHIGGNAQFDEIWIHEAEA
                     AGLANGVPNETLRGWFGPDRLRGPLPTHVDPATVAYPPTPPNGTLEGGEEMDLGDRCL
                     EVIHAPGHSPGGTCLWDERNRILFTTDVAYPCSLLVDTRDDLPVYHRTLERLAALEPA
                     PELVFGSHCDVEMPVAMLAAQRDAIAAIMDGREPDRELDGGVLRWGFDGFAVELG"
     gene            complement(1064..2278)
                     /locus_tag="VGR22_01775"
     CDS             complement(1064..2278)
                     /locus_tag="VGR22_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV2127327.1"
                     /translation="MGYSRTPELGDMARHQGRDAATAWWRVGVIVGVALVVALTAADI
                     FWLLVEPLTLILVAIVIAQALSPLVGFVEHLLPRGVAVIAVYLALVLLIAGLGWILVP
                     MLVSEGEGLLKDIPGMLTQARNSLNSISIPGQGESSNQVVARVESAVQGTADRLLSLP
                     MTIFNSLIDFIVVVFMSAYWLIFTPALRRFTLSLFPEEKRLWVDSVLDEMGDTMGGFV
                     RGTLIDAFIIGLAVYIGLTVIGIQYVLLLAILSAIGELIPIIGPILAAVPAVFVGFMD
                     SPRTALVVLVFFLVLQQVESNLLLPLVMRNQADVPPLLSLVAVVIGGALGGILAAILA
                     IPIFGALRILIVRVVAPAERDWTGVDDAPHEVGTEEEVAPDEIVEEVDEEKENGHQDR
                     AEAPRERSGRGR"
     gene            complement(2467..>2840)
                     /locus_tag="VGR22_01780"
     CDS             complement(2467..>2840)
                     /locus_tag="VGR22_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127328.1"
                     /translation="AEVRPYAYIAHAYAIDIRNMADARTSTEEQDRPRLDWNRMARPA
                     ENRSRTSGIDEWGSVACATGDTLDETSRAVALPELASSNPADATASAPPEAVATVDLF
                     HGKAVALAGLGPHPPLNGPLR"
BASE COUNT          519 a          987 c          896 g          438 t
ORIGIN      
        1 accctgcggc acaccgacca acaccgctcc tgcagcgggc ttgtggcatc agtgatgcgg
       61 cccgcgatcg cctggctgag tagcagtcgc cgaactccta ccccagctcg accgcgaagc
      121 cgtcgaaccc ccagcggagc actccgccat cgagctcgcg gtccggctcc cgcccgtcca
      181 tgatcgccgc gatcgcgtca cgctgggccg ccagcatcgc gaccggcatc tcgacatcgc
      241 agtgcgagcc gaacaccagc tccggcgccg gctcaagcgc ggccaggcgc tccagcgtcc
      301 ggtggtagac cggcaggtca tcgcgcgtgt cgaccagcag cgagcagggg taagccacat
      361 cggtcgtgaa caggatgcgg ttgcgctcgt cccagaggca ggtcccaccc ggcgagtggc
      421 cgggggcgtg gatcacctcg aggcagcgat caccgaggtc catctcctcg ccgccttcga
      481 gggttccgtt cggcggtgtc ggcgggtagg cgaccgtcgc cggatcgaca tgggtcggca
      541 gcggcccgcg cagccgatcg ggtccgaacc acccgcgcag ggtctcgttc ggcacgccat
      601 tggcgagccc ggccgcctcc gcctcgtgga tccagatctc gtcgaattgt gcgttgccgc
      661 cgatgtggtc ccagtgggca tgactgttga tgacgaccac cggcagcgtg gtgatccgct
      721 ccaccacggc gaggatgtcg ccgacgccca tgcctgtgtc gatcagcgcg gcccgctcga
      781 cgccgagcac gaggaacgac ttcacgttct cctcgtgcag cggttcctgg acgcgccaga
      841 ccccctcggc gggcgagtcc accgcgaacc atccctgctc cagctcaccc tcgcgaggcc
      901 agatcacgtt atcgctcacc gctgacccct cctcaggacc gtcacccgtc gcacggcctc
      961 ggcccaccgg gcgaatgatg ccatcccgaa ccagcagcac gcgggccaga cgatgcgcac
     1021 gcccatgacc ggcgacgcgt gacccgcggc aacggctgct cgttcagcgg cccctgcccg
     1081 acctctcacg cggagcttcc gcccggtctt ggtgcccgtt ctccttctcc tcgtcgacct
     1141 cttcgacgat ctcatccggg gccacttcct cttccgtgcc cacctcgtgt ggtgcgtcgt
     1201 ccacaccggt ccagtcccgc tcggcaggcg cgacgacgcg cacgatcagg attcgcagcg
     1261 caccgaagat cgggatcgcc aggatagcag cgaggattcc gccgagagcc ccaccgatga
     1321 cgacggcgac cagggacagc agcggaggca cgtcggcctg gttgcgcatg actaggggaa
     1381 ggagcaggtt gctctccacc tgctgcagaa caaggaagaa gacaaggacg acaagtgcgg
     1441 tccgagggga gtccatgaac cccacgaaaa ccgccggcac cgcagcaagg atcggcccga
     1501 tgatcgggat gagctcaccg atggcggaga gtatggcgag cagcaggacg tactggatac
     1561 cgatgaccgt cagaccgatg tataccgcca acccgatgat gaacgcgtcg atgagggtgc
     1621 cgcggacgaa gccgcccatc gtgtcaccca tctcatccag cacgctgtca acccacagcc
     1681 gcttctcctc cggaaacagg gacagggtga accggcgcag cgcgggcgtg aagatcagcc
     1741 agtaggcgga catgaacact acgacgataa aatcgatcag cgaattgaag atcgtcatcg
     1801 gcaacgacag cagccggtcg gcagtcccct gcacggcaga ctccacccga gcaaccacct
     1861 ggttcgaaga ttccccctgg cccggaatgc tgatggaatt gagtgagttg cgagcctgcg
     1921 tcagcattcc ggggatatct ttcaggaggc cttccccctc ggataccaac attggcacca
     1981 gaatccatcc aagtcctgcg atcagcagga caagcgcgag atagacggcg atgacagcaa
     2041 cgccacgcgg gagcaggtgc tccacgaagc cgacaagggg agacagtgcc tgcgcgatga
     2101 cgatggccac caggatcagc gtcaggggtt ctaccaggag ccagaagatg tcggccgccg
     2161 tcaacgccac cacgagcgcg acgccgacga tgaccccgac acgccaccag gccgtcgcgg
     2221 catcccggcc ctggtgcctg gccatgtcac ccagctccgg tgttcgtgag taccccatcc
     2281 actgctcctc gtctgttgta catcttttgg cacgcgggac tcgcccactg ccgacggctg
     2341 cggtgcgcag cgatgccttg cacgatcgcg gccacggatg tatgacggtg ctggagtagg
     2401 aatcgccgac ggcggctgcg cgccgtggtc cgcggcacgg acaggaaacg gcccgcccgg
     2461 tggtgatcac cggagcgggc cgttgagggg agggtggggg ccaagccccg cgagggccac
     2521 ggctttaccg tggaagaggt cgaccgtcgc cactgcctcc ggcggagcgc tggctgtcgc
     2581 atccgctgga ttcgagctcg cgagctcggg gagggccacg gcccgagagg tttcgtcgag
     2641 cgtgtcaccg gtcgcacagg cgacactgcc ccactcatcg atgccactgg tgcggctccg
     2701 gttttccgct ggccttgcca tccggttcca atcgagcctg ggtctatctt gctcctccgt
     2761 cgacgtgcgc gcatccgcca tattacggat atcgatcgcg tatgcatgcg cgatatacgc
     2821 atatgggcgg acctcagcac
//
LOCUS       DASYWL010000052         9281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_96296, whole genome shotgun sequence.
ACCESSION   DASYWL010000052 DASYWL010000000
VERSION     DASYWL010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9281
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96296"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VGR22_01785"
     CDS             complement(<1..737)
                     /locus_tag="VGR22_01785"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEV2127329.1"
                     /translation="MTDKHQVRHVGVVGLAVMGANLALNLARNGYAPAVYNRTAARTE
                     EFLADEAKGTEIIPAWSINELMASLERPRRILLMVKAGPAVDAVIEDLVPYLEQGDIL
                     IDGGNSFFQDTERRSQELADRGFHFVGMGVSGGEEGALWGPSLMPGGSAEAYAELEPM
                     LTAIAAKSDSGPCVTHVGPGSAGHYVKMVHNGIEYGDMQLIAEIYDIMRSALDMSAPE
                     IAEVFMRWNGGKLGSFLVEITATVLRE"
     gene            complement(804..1928)
                     /gene="tal"
                     /locus_tag="VGR22_01790"
     CDS             complement(804..1928)
                     /gene="tal"
                     /locus_tag="VGR22_01790"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEV2127330.1"
                     /translation="MTNTIKRLHSEHDQSVWQDDISRDMLLQGTLKQRIEEVGIRGVT
                     SNPTIFQKAISVGHAYDEDIRRMLGEDKTPVQVFDALAIKDIRDACDLFRPMYDESNG
                     GDGFVSIEVLPSLARDTEGTVENARMLWRAVDRPNLMVKVPGTVEGAPAIEQLLSEGI
                     NINVTLLFSLENYERVARAYVAALKQRDNAGESIDRIASVASFFVSRVDTAADKAIDE
                     KIAEGNTDLADLKGKVAVANARLAYRKFEEIFLGEEFMVLAEKGAGVQRPLWASTGTK
                     NPEYSDVLYVETLIGPHTVNTMPWPTIEAFLDHGTVKRTVDQHYDEAERVLARLEEAG
                     ISLDAITTKLEDDGIDAFAKSYDELLAGVEEKEKALAAAG"
     gene            complement(2004..4145)
                     /gene="tkt"
                     /locus_tag="VGR22_01795"
     CDS             complement(2004..4145)
                     /gene="tkt"
                     /locus_tag="VGR22_01795"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV2127331.1"
                     /translation="MATATTVDKALEEKAINSIRSLSMDAVQEANSGHPGLPLGAAPM
                     GYVLWQEYLKHNPRDPEWFDRDRFVLSAGHGSMLVYSLLHLTGYDVTLEDLKAFRQIG
                     SKTPGHPERGHTPGVEVTTGPLGQGMGNGVGMAMAERFLAAKYNRPDHEIIDHYTYGI
                     VSDGDIMEGVAFEAAAIAGTLKLGKLIYLYDSNHITLAASANVTLGEDVGARFEAMGW
                     HVQSIDGMDTDEVRRALDAARGETERPSLIVSSTVIGYGSPNKAGTFGAHGAPLGPDE
                     VEATKKNLGLPTDEKFFVYDEALDHFREALDRGAEAQAQWRMRWDAYQNAFPEEAAEL
                     ERAINGELPDGWDSDIPSWEPDPKGIATRKAGGAVMNAFFEKLPTVIGGSADLNPSTN
                     TVLKGGGDFMPPRFDEDDAAVQGASGGPWNYTGRNIHFGVREHGMGAIVNGMAAHGGV
                     IPFSATFLTFSDYQRPSIRLAALSKLRSTFVFTHDSIAVGEDGPTHEPVEHTMSLRLI
                     PDLQVFRPADANETADAWRVVIESGKASVLLLSRQDLPVQDRSGAKGGVREGAYILAD
                     SKGDPDLVFLATGSEVALAADARAELAQHGVDARVVSMPSWELFDAQDDAYRATVLGP
                     AGTPRVSVEAGTVIGWERYTGEKSAHIGIDRYGASGPGNAVLKYFGFSVENVTATALE
                     LLGRHEDAVPYRETADKHAAGPAPSGAEGHS"
     gene            complement(4311..5609)
                     /locus_tag="VGR22_01800"
     CDS             complement(4311..5609)
                     /locus_tag="VGR22_01800"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2127332.1"
                     /translation="MEPAAGVTPDHFLDTSAPRTAVVSWALYDLGNTTFSLAIVSLHF
                     PLWVVNDRGGTDAHFTLAVSLSMLLMFLAAPVLGALSDQASRRKPFLVTATLICVSFT
                     ALLGTGSIWLSLAFFVVANFAYQAGLLFYDTLLPSVATRENRGRISGFGVAVGYIGSI
                     IAILTALAILGVGGTDARVWVFRVTAVLFLLFALPLFRFVRERERPVSSAIDLGAARQ
                     AIRTALGAVASLRQRRDLRNFLLAHLLYADAANTAIAVLGIYATNEVGFSDEQAQMVL
                     LVGIVGAITGGLTIGHVADRIGPRDTLLGVLGMWLVSLTLVATIAWLDLSEAVFWPVA
                     VLVGIALGGLWSADRPLMLQLIPAGRLGEYYGLYSMVGRFAAMLGPLLWAVVVDGVGW
                     GRPTAVAGLAVFIAVAIVLLSRVDNRPVPQAQAVAGPVSP"
     gene            complement(5611..5913)
                     /locus_tag="VGR22_01805"
     CDS             complement(5611..5913)
                     /locus_tag="VGR22_01805"
                     /inference="COORDINATES: protein motif:HMM:NF023857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family antitoxin"
                     /protein_id="HEV2127333.1"
                     /translation="MSTPKPLIAFGYPTEAHGRIPSFTSVEEEAGFWDTHDFTDFEEE
                     LESFELVVEPDLSSSLTLVLATNDREELDRRAEEFGVAPSTLVPIWVKERLHKEAS"
     gene            complement(6265..6459)
                     /locus_tag="VGR22_01810"
     CDS             complement(6265..6459)
                     /locus_tag="VGR22_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127334.1"
                     /translation="MSFETLIWFGISGGTLAFAISLLWQRYVQRTERAVQSHGSFTMF
                     AILGIIFSIGTFVTALATMG"
     gene            6632..7093
                     /locus_tag="VGR22_01815"
     CDS             6632..7093
                     /locus_tag="VGR22_01815"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2127335.1"
                     /translation="MSDTGGGRSRREQRFTIEQLERMTGYPARTIRYYIARGLLQPAY
                     GRGPTATYDRDHLRRLQYIELLKQERMSLDQIREHLAEMEPADIEVALRGQFSPSADL
                     WRHLPIHEDLVVMIRDRQQGRRDPALDMAFDLIIEYARSVLEDLDRRVDHD"
     gene            7086..7655
                     /locus_tag="VGR22_01820"
     CDS             7086..7655
                     /locus_tag="VGR22_01820"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEV2127336.1"
                     /translation="MTDEERSITSGGGIVRGSQRSARGDTPQTPSDLTRAFRRRWATG
                     IAVMTLRDGGRLRGITLTAMMPLSLEPPLVAVGLTAGGEFPSLAKEGRRCVIAILVRD
                     QEFLSERFAGRAPVPDAAFTGVPHELDAHGVPILSGAAAHVTGGIVSRAAHGDHELVV
                     IEVDGGEIGPDEDDPLLTYEGAYRGLEVE"
     gene            7652..8002
                     /locus_tag="VGR22_01825"
     CDS             7652..8002
                     /locus_tag="VGR22_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127337.1"
                     /translation="MISDERDDLWIPDNPDGSGWADVDWVSATRGSRYHYRHGEAYEQ
                     GVRTLPPLFRVTTRLESEQIRPHVNTYYVPGPKLHDFVEELVWNGGAERIWAIEPSSK
                     APPEARVETEGTES"
     gene            8284..8448
                     /locus_tag="VGR22_01830"
     CDS             8284..8448
                     /locus_tag="VGR22_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127338.1"
                     /translation="MVRLVYVEVFSRADDTIAREKRVKRWRREKKAALIEKTNPQWHD
                     LAEVYGFEPL"
     gene            complement(8505..8783)
                     /locus_tag="VGR22_01835"
     CDS             complement(8505..8783)
                     /locus_tag="VGR22_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate o-methyltransferase 1"
                     /protein_id="HEV2127339.1"
                     /translation="MTDATPQQPILTEEHAAEVVWAAVERAGGTRSIYRNPRMAFATN
                     ARRKIEAEGYEVDIRYGEISTPAIATVEGWVFEIFDEEIELLMRPKGR"
     gene            8944..>9281
                     /locus_tag="VGR22_01840"
     CDS             8944..>9281
                     /locus_tag="VGR22_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV2127340.1"
                     /translation="MVTLANVREDVDEILPGAVADRRYLHENPELGFQEFNTSRFVAE
                     RLQTLGVEDIRTEVNVTGVTGLIRGTGDGSGKGKVVLARADMDALPIVEENEVDYRSK
                     NDGVMHACGH"
BASE COUNT         1598 a         2943 c         3150 g         1590 t
ORIGIN      
        1 atctcccgca gcacggtggc ggtgatctca acgaggaacg accccagttt gccgccattc
       61 cagcgcatga agacctcagc gatctccggg gccgacatgt cgagcgcgct acgcatgatg
      121 tcgtagatct cggcgatgag ctgcatgtcg ccatattcaa tgccgttgtg caccatcttc
      181 acgtagtgcc cggccgaacc gggaccgacg tgggtgacgc agggcccgga gtccgacttc
      241 gcggcgatcg cggtcagcat cggctccagc tcggcgtagg cctcggcgga gccaccgggc
      301 atgaggctcg gaccccagag agctccctcc tcgccgccgg aaacccccat gccgacgaag
      361 tggaagccgc gatcggcgag ctcctgtgag cggcgctcgg tgtcctggaa gaacgagttg
      421 ccaccgtcga tcaggatgtc gccctgctcg aggtacggca ccagatcctc gatcactgca
      481 tcgacggcgg ggccggcctt gaccatgagc aggatgcggc gcggtcgctc cagcgaggcc
      541 atcagctcgt tgatggacca tgccgggatg atctccgtgc ccttcgcctc gtcggcgaga
      601 aactcctccg tccgcgcggc agtccggttg tagacggcgg gggcgtagcc attccgcgcg
      661 aggttgagcg ccaggttggc acccatcacc gccagaccaa cgacaccaac gtgtcgcacc
      721 tggtgtttgt ccgtcatgcg tggtgctctc ccattcaggc agggagtcgc cggcgcgacg
      781 cctccccggt ccctgcgcca ggctcaacca gcggcggcca gcgccttctc cttctcttcg
      841 accccggcga gcagctcgtc gtaggacttc gcaaaggcgt cgatgccgtc gtcctcaagc
      901 ttcgtggtga ttgcgtcgag cgagatgccc gcctcctcga gccgcgccag cacccgctcg
      961 gcctcgtcgt agtgctggtc gacggtgcgc ttcaccgtgc cgtggtcgag gaacgcctcg
     1021 atcgtcggcc agggcatcgt gttgaccgtg tgcgggccga ttagcgtctc gacgtagagc
     1081 acgtcgctgt actcggggtt cttggtgccg gtgctggccc agagcggccg ctgcacgccg
     1141 gctcccttct cggcgagcac catgaactcc tcgccgagga agatctcttc gaacttgcgg
     1201 taggccagcc gtgcgttggc gactgcaacc ttacccttga gatcggcaag atcagtattg
     1261 ccctcggcga tcttctcgtc gatcgccttg tcggcggcgg tgtcgacacg actgacgaag
     1321 aacgacgcga ccgaggcgat gcggtcgatc gactcgccgg cgttgtcgcg ctgcttcagc
     1381 gcggcgacgt aggcacgggc gacccgctcg tagttctcca gcgagaagag cagggtcacg
     1441 ttgatgttga tgccctcgct gagcagctgc tcgatggcgg gtgctccctc gacggtgccc
     1501 ggcaccttca ccatcaggtt cgggcggtcc acggcgcgcc agagcatgcg ggcgttttcc
     1561 acggtgccct cggtgtcgcg ggcgaggctc gggagcacct cgatcgagac gaagccgtcg
     1621 ccgccgttgc tctcgtcgta catcggtcgg aagaggtcgc aggcgtcccg gatgtccttg
     1681 atggcgaggg cgtcgaacac ctggaccggc gtcttgtcct cgccgagcat ccgcctgatg
     1741 tcttcatcgt aggcgtgtcc gacggagatc gccttctgga agatcgtcgg gttcgaggtc
     1801 acgccccgga tgccgacctc ttcgatgcgc tgcttgaggg tgccctgcag cagcatgtcg
     1861 cggctgatgt cgtcctgcca gacactctgg tcgtgctccg aatggagccg cttgatcgtg
     1921 ttggtcatga tgccgtcctt tcgtgtcctg ctcagctcgg tttgttcatg gaaaccccat
     1981 gcaactcccg tgcccgcggc acatcaggag tggccctcgg cacctgacgg agctgggcca
     2041 gccgcatgct tgtccgccgt ctcgcgatac ggcacagcgt cctcgtggcg gccgaggagc
     2101 tcgagcgcag tggcggtgac attctcgacg ctgaagccga agtacttgag caccgcgttg
     2161 cccggaccgg aggcgccgta gcggtcgatg ccgatgtgcg cactcttctc accggtgtag
     2221 cgctcccagc cgatcaccgt gccagcctcg acggagacac gcggcgtgcc agccgggcca
     2281 agcacggttg ccctgtaggc atcatcctgt gcgtcgaaga gctcccagct cggcatgctc
     2341 accacccgcg catcgacgcc gtgctgcgcc agctctgcgc gcgcgtcggc ggccagcgcg
     2401 acctcggagc cggtggccaa gaagacgagg tcgggatcgc ccttgctgtc ggcgagaatg
     2461 taggctccct cgcgcacgcc acccttcgcg ccggagcggt cctgcaccgg cagatcctgt
     2521 cgactgagga gcagcacgct cgccttgccg ctctcgatca ccacgcgcca ggcgtcggcc
     2581 gtttcgttgg catcggctgg gcggaagacc tgcaggtccg ggatcagccg cagcgacatc
     2641 gtgtgctcga ccggctcgtg cgtcgggcca tcctcgccga cggcgatgct gtcgtgggtg
     2701 aagacgaagg tcgagcgcag cttgctgagc gcggcgaggc ggatcgacgg ccgctggtaa
     2761 tccgagaagg tcaggaaggt tgcactgaac gggatcacgc cgccatgcgc agccataccg
     2821 ttgacgatcg cccccatacc gtgctcacgg acaccgaagt ggatgttgcg gccggtgtag
     2881 ttccaggggc cgccgcttgc gccctgcacc gccgcgtcgt cctcgtcgaa cctgggtggc
     2941 atgaagtcgc cgccgccctt gaggacggta ttggtcgacg ggttgaggtc ggcggagccg
     3001 ccgatgacgg tcggcagttt ctcgaagaac gcgttcatca cggcaccgcc cgccttgcgg
     3061 gtggcgatgc ccttggggtc cggctcccag gatgggatat cgctgtccca gccgtcgggt
     3121 agctcaccgt tgatcgcccg ctccagctcg gcggcctcct ccgggaacgc attctggtag
     3181 gcgtcccagc gcatacgcca ctgggcctgc gcctcggcgc cgcggtcgag cgcctcccgg
     3241 aagtggtcca gcgcttcgtc gtagacgaag aacttctcgt cggtcggcag accgaggttc
     3301 ttcttggtgg cctccacctc gtccgggccg agcggcgcgc catgtgcacc gaacgtgccg
     3361 gccttgtttg gcgagccgta gccgatgacg gtgctggaga cgatcagcga cgggcgctcc
     3421 gtctccccgc gggcggcgtc gagcgcgcgg cggacctcgt cagtgtccat gccgtcgatg
     3481 gactgcacgt gccagcccat cgcctcgaag cgggcaccga cgtcctcgcc aagcgtgacg
     3541 ttggccgatg ctgcgagcgt gatgtggttg gagtcgtaga ggtagatcag cttgccgagc
     3601 ttgagggtcc cggcgatcgc ggccgcctcg aaggcgacgc cctccatgat gtcaccgtcc
     3661 gagacgatgc cgtaggtgta gtggtcgatg atttcgtggt ccgggcggtt gtacttcgcc
     3721 gccaggaacc tctcggccat ggccatgccc acgccgttgc ccatgccctg cccgaggggg
     3781 ccggttgtca cctcgacgcc cggggtgtgg ccgcgctctg ggtggccggg ggtcttcgag
     3841 ccgatctggc ggaaggcctt gagatcctcg agcgtgacgt cgtaaccggt caggtggagc
     3901 agcgagtaga ccagcatcga gccgtggcca gcggaaagca cgaagcggtc gcggtcgaac
     3961 cactctggat cacgcgggtt gtgcttcagg tactcctgcc agagcacgta tcccatcggc
     4021 gccgcgccga gtggaagccc cgggtggccg gaattggcct cctggacggc gtccatcgaa
     4081 agcgagcgaa tgctgttgat cgccttctct tcgagtgcct tgtcaacggt tgttgccgta
     4141 gccaaatccg tgtctccttc gatgtttcag gtcgaatcgc gctgggcgac accattcgcc
     4201 ggtcaggagg cagcatggtg gcggtcacgg gaatgcaggc cggcttgcac cggcggtcac
     4261 aaccttcccg gttcgttgca ggtattatcc cacgccggtt caatgcggag ttatggtgat
     4321 accgggcctg ccaccgcctg ggcctgcggt accggccggt tgtcgacgcg gctcagcagc
     4381 acgatcgcga cggcgatgaa gacggcgagt ccggcgacag cggtcgggcg tccccaacca
     4441 accccgtcaa ccaccactgc ccagagcaac gggccgagca tggcggcgaa ccggccgacc
     4501 atcgagtaga gaccgtagta ctcgccaagg cggccggcgg ggatgagctg gagcatcagc
     4561 gggcggtcag ccgaccacag cccgcccagg gcgatgccga ccagcaccgc cacgggccag
     4621 aacaccgcct cggacaggtc cagccaggcg atggtcgcga ccagcgtgag ggagaccaac
     4681 cacatgccca gcacgcccag gagggtgtcg cgagggccga tgcggtcggc cacgtggccg
     4741 atggtgagcc cgccagtgat cgccccgacg atgccgacca gcagcaccat ctgcgcctgc
     4801 tcgtcggaga agcccacctc gttggtcgca taaataccga gcaccgcgat ggcggtgttg
     4861 gcggcgtcgg cgtatagcag gtgcgcgagc aggaagttcc gcaggtcacg gcgctggcgc
     4921 agcgacgcca ccgcgccgag ggcggtacgg atagcttgcc gggcggcacc gaggtcgatc
     4981 gcgctgctga ccggccgctc gcgttccctg acgaagcgga agagcgggag tgcgaagagc
     5041 agaaagagca ccgcagtcac gcggaagacc cagacgcggg cgtctgtgcc accgacaccg
     5101 aggattgcca gcgcagtgag gatggcgatg atcgagccga tgtagccgac cgcgacgccg
     5161 aacccgctga tacgcccgcg gttctcgcgg gtagcgacgg agggcagcag cgtgtcgtag
     5221 aagaggagcc ctgcctgata ggcgaagttg gcaaccacga agaaggcgag cgacagccag
     5281 atgcttcccg tgccgagcag cgcggtgaag gagacgcaga tcagggtggc ggtgacgaga
     5341 aagggcttgc ggcgtgatgc ctggtcggag agggcgccga gcacgggcgc ggcgaggaac
     5401 atgagcagca tcgagaggct cacggcgagc gtgaagtgcg catccgtgcc gccgcggtcg
     5461 ttgacgaccc agagcgggaa gtggagggag acgatcgcga ggctgaaggt cgtgttgccg
     5521 aggtcgtaga gcgcccagga gacgaccgcc gtgcgtggtg ccgaagtgtc caggaagtgg
     5581 tcgggtgtga ccccggcggc tggctccatg ttatgaggct tccttgtgga gtcgctcctt
     5641 gacccaaatc ggcacgagcg tcgacggcgc taccccgaac tcttcggcgc gacgatcgag
     5701 ttcctcgcga tcgttcgtcg ctagaactag ggtcagtgag cttgaaagat caggctcgac
     5761 gaccagctca aatgactcca gttcttcttc gaagtcagtg aagtcatggg tatcccagaa
     5821 accggcttct tcttcaaccg aagtgaacga cggaatgcgg ccgtgcgcct cggttgggta
     5881 gccgaaggcg atcaatggct tgggggtcga catgatcgcc ttctcctcca ggtgcttggt
     5941 cctccgctat tatgccggaa gaaagaaatc tcgtgtcacc cgaccgcgcg atagaacagc
     6001 gctctcgctg gcccttggcg tcagcagccg cgcccaaacg tacttgccgt catgctgagc
     6061 gagcgccgca ggtgcgaagt cgaagcatcc ccgcgcgaag cgcaacgaag tggtagacag
     6121 attatgagta ttgagtccag cgcgaggcga aaacgacagc aagctggctg cgcttggcat
     6181 gagggcttct cgacaagctc ggagtgacgg tcggtgggtg gcgtgttgtc gctatccctg
     6241 ccaatgaacc tacggcaacg gtcatcatcc catcgtcgcc agcgcggtca cgaaggtccc
     6301 gatggagaag atgatcccga ggatcgcgaa catcgtgaac gaaccgtggc tctgcaccgc
     6361 gcgctcggtg cgctgcacat agcgctgcca cagcagcgag atcgcgaagg cgagcgtgcc
     6421 gccgctgatg ccgaaccaga tcagggtctc gaagctcatt cgttcgggtt tcctgtgctg
     6481 tgggtggatg cctgcgtggg gcgagataac cacatgccgt cgcgatgata ctacgctgcc
     6541 cctgtgaatc ccggtccctg cacgctcgaa acgataccgg acgtggcatc atgggcgggt
     6601 gatgacccac ggcccagcag cgaggagcgg aatgtcggat acgggcggcg gacgatcacg
     6661 gcgagaacag cgcttcacga tcgagcagct ggagcggatg accgggtacc ccgcccggac
     6721 catccgctac tacatcgcgc gcggactcct tcagcctgcc tacggacgcg gcccgacggc
     6781 cacctacgac cgtgatcacc tcagacggct gcagtacatc gagctgctca agcaggagcg
     6841 gatgtcgctc gaccagattc gggagcacct cgccgagatg gaaccggccg acatcgaggt
     6901 cgcgctgcgc gggcagttca gcccctcggc ggacctctgg cggcacctgc cgattcatga
     6961 ggacctggtg gtgatgatcc gcgatcggca gcagggacga cgtgacccgg ccctcgacat
     7021 ggcgttcgac ctgatcatcg agtacgcacg gtcggtgctg gaggatcttg acaggcgggt
     7081 ggaccatgac tgacgaggag cgcagcatca ccagcggcgg cggcatcgtg cgcggtagcc
     7141 agcgctcggc tcgcggcgac actccgcaga cgccctcgga tctgacgcga gccttccggc
     7201 ggcgctgggc cactggcatc gcagtgatga ccctgcgcga cggcgggcgc ctgcggggca
     7261 tcaccctgac agcgatgatg ccgctctcgc tggagccgcc gctggtcgcg gtcggcttga
     7321 cggcgggcgg cgagtttccg tcactggcga aagaggggcg gcgctgcgta attgcgatcc
     7381 tcgttcgcga tcaggagttc ctctcggagc gcttcgccgg gcgcgctcct gtgccggacg
     7441 ccgccttcac cggcgttccg cacgagctgg acgcccatgg ggtgccgatc cttagcggcg
     7501 cggccgccca cgtgaccggc gggatcgtct cccgagcggc tcacggtgac cacgagctgg
     7561 tggtgatcga ggtggatggc ggggagatcg gccccgacga ggacgatccg ctgctgacct
     7621 acgagggcgc ctatcgcgga ctggaggtcg agtgatatcg gacgagcgtg acgatctctg
     7681 gatccccgat aacccggacg gcagcggctg ggccgatgtc gactgggtga gcgcgacccg
     7741 cggcagtcga tatcactacc ggcacggcga ggcgtacgag cagggtgtca ggacgctgcc
     7801 gccactcttt cgggtgacca cgcgactgga atcggagcag atccgaccgc acgtgaatac
     7861 ttactatgtg cccggcccga agctacacga tttcgtcgag gagttggtct ggaacggcgg
     7921 cgccgagcga atctgggcca tcgaaccgtc atccaaggct ccccccgaag cccgggttga
     7981 gaccgaaggt actgagagct gaacctgggt gcacgtctcc gtgaatgagc cctgccatta
     8041 cgaccggagc gcagctcggc gtgcggagtg gagaaatctc tcccacgaga ggaagcagag
     8101 ttctctcggc gagacaactc gcgaaaagcg tttgttggga ggggaggatg cagcggacct
     8161 actacgtgta catcctcgcc agtaagactc ggcggattta cattggcatc acgagtgacc
     8221 tcgagcggcg ggtattggga acaccgcaac ggggtacggt cttcattcgc ttccgggtac
     8281 aacatggtcc gcttggtcta tgtcgaggtg ttcagcagag cggacgacac catcgcgcgg
     8341 gagaagcggg tcaaacggtg gcgccgggag aagaaggccg cactcatcga gaaaaccaac
     8401 ccgcagtggc acgacctcgc cgaggtctac ggcttcgagc cgctgtaatc ggccgaaatg
     8461 acgaggtatc gccttgcaaa tggcggaccg gcatcggtga gaatctaacg tcccttcggg
     8521 cgcatgagca actcgatctc ctcatcgaag atctcgaaca cccatccctc gacggtcgcg
     8581 atcgccggtg tggagatctc cccgtagcgg atgtccacct cgtacccctc ggcctcgatc
     8641 ttgcggcgcg cgttcgtggc gaaggccata cggggattgc gatagatgga acgggtgccg
     8701 cctgcccgct cgaccgccgc ccagacgacc tcggccgcgt gctcctcggt caggatcggc
     8761 tgctggggtg tggcgtcggt catggggctc gtctccggtc gggaatgcgg gatggaagcg
     8821 gcggggttcg accttgcggg atcggtcgcg tctgctatgt tgcccactga agatacacgc
     8881 attccggcct cgcggtcgct ccgacccgcg accggcgcaa cacccggtga ggtacagcaa
     8941 cgcatggtga cactggcgaa cgttcgtgaa gacgtggacg agattctgcc cggcgcggtt
     9001 gccgaccggc gatacctcca cgaaaacccc gagcttggct ttcaggaatt caacacttca
     9061 cggtttgtgg ccgagcggct ccagaccctc ggcgtcgagg acatccgtac ggaggttaac
     9121 gtcactggcg tgaccggcct gattcgcggc accggcgatg gttccggcaa gggcaaggtc
     9181 gtgctggcgc gggccgacat ggacgcgctg ccgatcgtcg aggagaacga ggtcgactac
     9241 cgctcgaaga acgatggtgt gatgcatgct tgcgggcacg a
//
LOCUS       DASYWL010000053         2959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_171418, whole genome shotgun sequence.
ACCESSION   DASYWL010000053 DASYWL010000000
VERSION     DASYWL010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171418"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VGR22_01845"
     CDS             <1..267
                     /locus_tag="VGR22_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127341.1"
                     /translation="SVMPMVYTTIAEGEPWSWDRLIEEHLDALPEGTCWLPLYTEHNE
                     AMLKRQARLAREREMIGVAWYSAGLIQTYDRWPVVRSTVNALAD"
     gene            456..1748
                     /locus_tag="VGR22_01850"
     CDS             456..1748
                     /locus_tag="VGR22_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HEV2127342.1"
                     /translation="MTDGAVREGEQQYDLVISGGHVIDPANGVSMVGDVAVRDGKIAA
                     VETHLDPASARRQIDAGGHYVTPGLVDLHTHIYWGVTYWGIEADPVAARTGVTTWLDV
                     GSAGGYTFPGFREYIVEKTQARVYALLNLSSIGLVAPTWEFSNPDYLDVDLAAGIVER
                     HRDIILGIKARIDSNTTRGTGILPMQRARELADRVGLPLMTHIGVSPPSLSEVAEYLR
                     PGDILTHCFTGRDMRIIGPDDKIDPQVKALKEQGLVLDVGHGTGSFSYEVAEAMLDQG
                     IPPDVISSDIHQMAIQGPMFDLPTTLSKFINLGMPIEDVIAAATINAARAVKLDHLGR
                     IGVGDVADVAIFWMEEGDYAFHDVQMLERKGSKLLVNTATLVAGQELPRAEERPLHFW
                     AQVPEVQRAAVFPGAAWDVEPAGDAEAVAEQMYAGYDE"
     gene            2003..2905
                     /gene="murI"
                     /locus_tag="VGR22_01855"
     CDS             2003..2905
                     /gene="murI"
                     /locus_tag="VGR22_01855"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEV2127343.1"
                     /translation="MAPAHTIATSDHPVGVFDSGLGGLSVVREIQRRLPCEDLIYFAD
                     NAWCPYGIRSPEEVRQRSEYVVGTLINAGAKAIVVACNTASAMAIFHLREAFPDVPLI
                     GLEPAVKPAVECTVTGTIGVLATPRTVAGERLQWLIETYAGGVEVYRVAATGLVELVE
                     AGVLAGDEVDAALRPLLDPMIAVGVDIVVLGCTHYPFLRGAIERYLGSGVEVLDSGEA
                     IARRLDVVLGQRRLRSLAIAPGTTRFTTSGDPATVGPVATLLLGHPVTVVASESEPVA
                     VEEPSFDAVGHLAPAHDLADLPNP"
BASE COUNT          510 a          904 c          998 g          547 t
ORIGIN      
        1 agcgtgatgc cgatggtcta cacgacgatc gccgagggcg agccgtggag ctgggatcgc
       61 ctgatcgagg aacacctcga cgcccttccg gagggaacat gctggctacc cctgtacaca
      121 gagcacaacg aggcgatgct caaacggcag gctcgtctgg cgcgtgagcg agagatgatc
      181 ggggttgcgt ggtactccgc cgggctgatc cagacctacg atcgctggcc ggtcgtgcga
      241 tcgaccgtca acgcgctggc cgactgaggc gttccacttg tcgatcaccg agtgctgacc
      301 tctggcctcg tactcgtggc gaagcatcgc catgcacagc gctggcgctc ttgcgtggtt
      361 tcgcccaata ggtatcctgc cgcaagaatt cggtcagcgt cgctggctgg cgaactgagg
      421 cgccgcgggc gccgacgaga gagtggagag caatggtgac cgacggcgca gtgcgggaag
      481 gtgaacagca gtacgacttg gtgatctccg ggggacacgt gatcgacccg gcaaacgggg
      541 tcagcatggt gggtgacgtt gcggtccgtg acggcaagat cgccgcggtg gagacgcatc
      601 tcgacccggc cagcgcgcga cgacaaatcg atgccggtgg tcactacgtc acgccgggcc
      661 tggttgacct gcacacgcat atctactggg gcgtgaccta ctggggcatc gaagcggatc
      721 cggtcgctgc ccgaaccggt gtgaccacat ggctcgatgt cgggagcgcc ggcggctaca
      781 catttcccgg gttccgtgag tacatcgtcg agaagacgca ggcgcgcgtg tacgcgctgc
      841 tcaatctctc ctcgatcggt ctcgtcgcgc ccacttggga gttttccaat cccgactatc
      901 tggatgtcga tctcgccgcg ggtatcgttg agcggcaccg cgatatcatt ctcggcatca
      961 aggcccgtat cgatagcaac accacgcgcg gcaccggtat cctgccgatg cagcgcgctc
     1021 gggagctggc ggaccgggtt ggcctgccgc tgatgaccca catcggtgta tcgccgccat
     1081 cgctttctga agtggcggag tacctgcggc ctggcgatat cctcacgcac tgcttcactg
     1141 ggcgtgacat gcgcatcatc ggaccggatg acaagattga tccgcaggtc aaggcactca
     1201 aggagcaggg gctcgtgctc gacgtcgggc atggtaccgg ttcgtttagc tacgaggtgg
     1261 ccgaggcgat gctcgatcag ggcatcccgc ccgacgtgat ctccagcgac atccatcaga
     1321 tggcgatcca ggggcccatg ttcgacctgc ctaccacctt gagcaaattc atcaatctcg
     1381 gcatgccgat cgaggatgtg atcgccgccg cgaccatcaa cgcggcccgc gcggtcaaac
     1441 tcgatcatct cggcaggatc ggcgtcgggg atgtcgccga cgtggcgatc ttctggatgg
     1501 aggagggcga ctacgccttc cacgatgtcc agatgctgga gcgcaagggg tcgaagctgc
     1561 tggtcaacac ggccacactg gtggccggcc aggagctccc gcgcgcagaa gaacggccgc
     1621 tgcacttctg ggcgcaggtg ccagaggtcc agcgagcagc cgtattcccc ggcgccgcgt
     1681 gggacgttga gccggctggc gatgcggaag cggtcgccga gcagatgtac gcggggtacg
     1741 acgagtagcg caaaggcatg gcgccgaggc tgagttccga acgcagccgg cacaggtgcc
     1801 gctctgagtt gggaaggtgg ccggaagccg ttccctgtcg atgagtcttc ccaccttgct
     1861 tcctgacagg gccgggttcc tgtcggattt cacccaagtg aagtggccgt acaggtctga
     1921 catggaacat aatggctcac tcgcctgtgc aggactgcgg gggggtgtac tcgtcgggtg
     1981 atgtatggtc ttgagggaca gcatggcacc agcacacacc atcgccacca gcgaccatcc
     2041 ggtcggcgtg ttcgattccg ggctcggcgg tctcagcgtt gtccgggaga tccagcgacg
     2101 gctaccctgc gaagacctca tctacttcgc cgacaacgcg tggtgtccct acggcattcg
     2161 gtcgccggag gaggtccggc agcgatcgga gtacgtcgtt ggcaccctca tcaacgctgg
     2221 ggcgaaggcc atcgtggtcg cctgcaacac ggccagcgca atggccatct ttcatctgcg
     2281 tgaggccttt cccgatgttc cgctgatcgg cttggagcca gcagtgaagc cggctgtcga
     2341 gtgcacggtg acgggcacca ttggcgtgct ggcgacccca aggacggtgg ccggcgagcg
     2401 cctgcagtgg ttgatcgaga cctatgccgg tggcgtcgag gtgtatcggg tggcggcaac
     2461 gggactcgtc gaactggtcg aggcgggcgt gctcgctgga gacgaggttg atgctgcact
     2521 gcgcccgctc ctggatccga tgatcgccgt cggcgtggat atcgtggtct tgggctgcac
     2581 gcactatccc ttcctgcgcg gtgcaatcga gcgctatctc ggttctggag tcgaggtgct
     2641 ggatagcggg gaggcgatcg ctcgtcgcct cgatgtcgtg ctcggccagc ggaggctccg
     2701 tagcctggcg atcgccccgg gaacgacgcg gttcacgacc agcggcgatc cggcaaccgt
     2761 gggaccggtc gcgacgctcc tgcttgggca ccccgtcacg gtggtcgcct cggaatccga
     2821 accggtcgct gtcgaggagc cgtcgttcga tgcggtcggc catttggctc ccgcccacga
     2881 cctggcggat ctgcccaatc cgtaatggca gcacgcgcgc cttcatctcg atttcatctc
     2941 gaccgagtac cgtcgtcag
//
LOCUS       DASYWL010000054         3627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_584410, whole genome shotgun sequence.
ACCESSION   DASYWL010000054 DASYWL010000000
VERSION     DASYWL010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584410"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2351)
                     /locus_tag="VGR22_01860"
     CDS             complement(<1..2351)
                     /locus_tag="VGR22_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2127344.1"
                     /translation="MSPFVGRTHEAASIIGLLRRGDVRLVTLTGPGGVGKTRLALEVA
                     RSIVEDFPDGVRFVALAPITNPGLVASSVAQVLGVRELGEDPPVVRLEMFLRDKHLLL
                     LLDNFEHVVEAAPLAADLLAACPDLKILTTSRVRLRLSGEREYPVPPLALPAAGHSLN
                     REGVSESEAVRLFTERAQAVKPDFALIDENADAVAEICHRLDGLPLAIELAAARVKVL
                     HPTALLSRLEKRLPLLSGGGRDLPARQHTMRDTISWSYDLLSPDEQALFRRLSVFAGG
                     FTLEATEVAVAPHDPGIDLFEGVASLMDRSLLRQAQAPDSEPRYSMLETVREYGLEQL
                     DSHGEADAVRWCHAAYFLALVEQVDPLLRTGEQQTWLGRLEADHANLRVALGWAIERG
                     EAEMALRLAGALHWFWYLRGHWNEGRGWLKRALELPSHGSGVAVRVKALTGAGVLAFA
                     QSDYPAARAQLQESLAICRQRGDPAGSAYALHFLGMAALLPGQYAVARDLFEESVEGF
                     RRAEDPWGLAMSLCSLGVAVMQMEADGAGDHDDARVFFEESLALFRERGDTFGLARAL
                     QYVGEAARALGDYPASRKLYEESLTRYRALDHRNTAASVVHNLGYVAQHQGDLQGAAS
                     CFAEALAVHVEHGDRRNIGHCLGGLAGMVGLLGPSEQAARLFGAADAMFAGTGASMFI
                     VDRPDYDRNLAMIRDRLGEQQFAAAFAAGQMLPLDDALAEAAQATAMVKVESSGASAN
                     GTPTAVSCQSATDWYGLTAREREVLQLLVDGRTDRAIAEALFI"
     gene            3326..>3627
                     /locus_tag="VGR22_01865"
     CDS             3326..>3627
                     /locus_tag="VGR22_01865"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2127345.1"
                     /translation="MTRGRGRQPIRLGIIGTGLAVEQLHWPALKRMPDRFRVAAFCDV
                     ERSNAERFADYSGAAMDAYVEDFHDLLRRDDVDAVLVSLPIPLNYPVTRAALEAGK"
BASE COUNT          700 a         1179 c         1154 g          594 t
ORIGIN      
        1 ctgatgaaca aggcctcggc gatcgcgcgg tcggtgcggc catcgacgag taactgcagc
       61 acctcgcgtt cgcgggcggt gaggccatac cagtcggtcg cggactgaca cgatacggcc
      121 gtcggtgtac cgtttgcgga cgctcccgag gactcaactt tcaccatggc ggtcgcctgc
      181 gccgcctcgg caagcgcatc gtccagcggt agcatctgac ccgccgcgaa ggcggcggcg
      241 aactgctgct cgccaagccg atcgcggatc atcgccaggt tgcggtcgta gtcggggcga
      301 tcgacgatga acatagaggc accggtgccg gcgaacatcg cgtccgcggc gccgaacagc
      361 cgcgccgcct gctcggacgg cccgagcagg ccgaccatcc cagccaggcc accgagacag
      421 tggccaatgt tccgccggtc gccgtgctcc acgtgcacag ctaatgcttc agcgaagcat
      481 gaagccgcgc cctgcaaatc gccctggtgt tgggcaacgt acccgaggtt gtggacgacg
      541 ctcgcggcgg tgtttcgatg gtccaacgcc cggtagcgcg tgagactctc ctcatagagt
      601 ttcctggatg ctgggtaatc tcccagggct cgcgctgcct cacctacata ctgcagtgct
      661 cgagcaagtc caaacgtatc tcctcgttct cggaacaagg ccaggctctc ctcgaaaaag
      721 acacgtgcat cgtcatggtc cccagcaccg tcagcctcca tctgcatcac cgcgacaccg
      781 agcgagcaga gggacatggc aaggccccaa ggatcctccg cccggcggaa cccctcgacg
      841 ctctcctcaa agaggtcgcg cgccaccgca tattgacccg ggaggagcgc cgccatgcca
      901 aggaagtgca gggcgtaggc actgccggca ggatcaccac gttgccggca gatggcaagg
      961 ctctcctgaa gttgagcacg tgcggctggg tagtcgctct gggcgaaggc gagtaccccc
     1021 gcgccagtga gcgccttcac ccgtaccgcg accccgctgc catgagacgg caactccaga
     1081 gctcttttca gccagccgcg cccctcattc caatgtcccc gcaagtacca gaaccagtgc
     1141 agggcaccgg ccaaccgaag cgccatctcg gcctcgcccc tctcgatcgc ccagccgagc
     1201 gccacccgca ggttcgcgtg atccgcctcc aatcgaccca gccacgtctg ttgctcccca
     1261 gtacgcagca atggatcgac ctgctcgacc aatgccagga agtaggccgc atggcaccac
     1321 cgcacggcgt ccgcctctcc gtgcgagtcc aactgctcca agccgtactc ccggacagtt
     1381 tccagcattg agtaccgggg ctcgctgtcc ggcgcctgtg cctgccgcag caggctccta
     1441 tccattagcg aggcgacgcc ctcgaagaga tcgataccag gatcatgcgg tgcgactgcg
     1501 acctctgtcg cctccagcgt aaagccgccg gcgaacactg acaaccgacg aaagagtgcc
     1561 tgctcatccg gggacaagag gtcgtaactc caggagatgg tgtcccgcat cgtgtgctgc
     1621 cgagcgggaa gatcgcgtcc accgccggac aagagcggca gccgcttctc caaccgggat
     1681 agcagcgccg tggggtgcag gaccttgaca cgggcagcag ccagctcaat ggctagggga
     1741 aggccgtcca gacggtgaca gatttccgcc accgcgtcgg cgttctcgtc gatcaaagcg
     1801 aagtcgggct tgaccgcctg cgctcgctcg gtgaacaacc gaaccgcctc ggactcggac
     1861 accccctctc ggttgagcga gtgtccggcc gctggcaatg ccagcggcgg caccgggtat
     1921 tcgcgttcac cggagagccg aagccggaca cggcttgtgg tcaggatctt caaatcagga
     1981 cacgcggcga gcaagtcggc cgccagcggt gctgcctcaa caacatgttc gaagttgtcg
     2041 aggaggagca ggagatgctt gtcacggagg aacatctcga gtcggacgac gggagggtcc
     2101 tcgccaagct cgcgaacgcc gagcacctgg gcgaccgagg aggcgacgag gccaggattg
     2161 gttatggggg caagggcgac aaaccgaacg ccgtccggga aatcctcgac aattgatcgc
     2221 gccacttcca gcgcgagccg cgtcttcccc accccaccgg ggccggtcaa ggtgacgagt
     2281 cgtacatcgc cccggcgcag caggccgata atcgatgccg cctcgtgcgt ccggccaacg
     2341 aagggactca acggggctgg tagggcagtg gtacggcgaa acggtgacac ggcggcgacc
     2401 atggcagaac ctaagcagtc cgttcggacg gatctcgaat gtatcgtgga acagaattat
     2461 gacgaactgc ccgggcccgg tcaatagttg cgatcagcaa ccgcagaaga cttggttgga
     2521 atacgcttgg aatcggaatt gatccgccgt cgagagcacc cgttttaccc atcgcaacaa
     2581 ggttcaaaga gcggtcaccc ccgttacact ccggtcccgg attgacgcga ttcagagatc
     2641 tcgtcccggc gcacgaccgg tgttgccagc tggtggcaag ggttggcgcg cgcaaggtca
     2701 cgaggtggag cggcaacagg cggcgaaatg atcgactgag gggcggatgg cgctgaatcg
     2761 gaagcgagcg tggggggaag tcgccgacga cgcgcgtcca agtcccttcg gcccgtcgat
     2821 gtgctctcgc tcactgccgg tcggtgccgt agtgatcgcg ccgatgtcaa tccttttgcc
     2881 tgcgccggtc atggagaccc atgagcggta acggcccaga agcaaagtcg cttccgaaga
     2941 cgccttcttg atgccgtcca ggttcacgac ggtggagatt caccgaccga gcaatgaaca
     3001 ggcggcgggt catgttgcgt gagggatgct ggagtcgctg gtcgtttcct gcaatcgcac
     3061 ggatcgggtc tacggttcgc cttacgcccg catcggcgcg tactatccgc cactggcctt
     3121 catccaaaac tcaacggctc gtctagtcca tgtggcaccg agccagcacc atgcattggc
     3181 atggtccgtg caaccatcct cggtccagta gcgcaaggag accatcgcgc agttaccgag
     3241 cacattcggc agcggtacgc cgtgttgtgg actgcctgct ggacgcattc tattcgccca
     3301 catggcattg tcggggaggg tcgagatgac gaggggtaga ggtaggcagc cgatccggct
     3361 ggggatcatc gggaccgggc tggccgtgga gcagctacac tggccggcgc tgaagcggat
     3421 gcccgaccgg tttcgggtgg cggcgttctg cgacgtggag cgctccaacg ccgagcggtt
     3481 tgctgactac agcggcgcgg cgatggacgc ctacgtggag gacttccacg acctgctccg
     3541 acgcgacgac gtggacgccg tgctcgtctc gctcccgatc ccgctcaact accccgtgac
     3601 ccgggcggcg ctggaggccg ggaagga
//
LOCUS       DASYWL010000055         6940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_302588, whole genome shotgun sequence.
ACCESSION   DASYWL010000055 DASYWL010000000
VERSION     DASYWL010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6940
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302588"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /gene="larC"
                     /locus_tag="VGR22_01870"
     CDS             <1..337
                     /gene="larC"
                     /locus_tag="VGR22_01870"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nickel insertion protein"
                     /protein_id="HEV2127346.1"
                     /translation="MKKGRPATKVSLLAPASRQAQLEHVLMFNTTTLGTRSWSIERTK
                     AGRDFGAVTTRWGDVPVKLRILDGRVIDAQPEYDEIARLAREFDQPIRDVWNEAHRLG
                     ESFVGQPRR"
     gene            complement(334..774)
                     /locus_tag="VGR22_01875"
     CDS             complement(334..774)
                     /locus_tag="VGR22_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127347.1"
                     /translation="MRRILFAMLLALHGAVHLMGPVDFWGIADLEEIAPPDASTAVVD
                     LLGGLWLLAAVSFIVGAVALWRGARWWRSLVLATAVLSFVIAGAVWADAWVGMALDVA
                     IAVIALPGRLIPSPDRDPVTPMDGTVARTHVTGRRPATRRIAPR"
     gene            complement(963..1550)
                     /locus_tag="VGR22_01880"
     CDS             complement(963..1550)
                     /locus_tag="VGR22_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B-box zinc finger protein"
                     /protein_id="HEV2127348.1"
                     /translation="MAQQFAAPTTGEVPCSYHPDVMTGLRCSRCGKPICPRCAVRTPV
                     GLRCPDCAGVRGLPTIRTSPNALARAGGLAMAVAALVTALWYLAPDWKFYLSLALGFG
                     VAEAIAWIAKGKRGSDLQVLGIAIVTLAMVAVRLLLAQKYGIPIDLMLSSERVVVQEG
                     GQLLAGMPRDFVQLRIVPDILFMALTWVIVWVRFR"
     gene            complement(1672..2838)
                     /locus_tag="VGR22_01885"
     CDS             complement(1672..2838)
                     /locus_tag="VGR22_01885"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEV2127349.1"
                     /translation="MDTATAILHLLFLAGMLLIIVGGLLAILLLWYSRGRDPHIGPVA
                     EYLSEPPDDLPPGAVGTLIDEHADEHDVMATLLGLARHGAVEITQVSENGRRDFVLML
                     IHPRRIEDRIERDLLVVLFGPDPQALQEVRLSDVRGVFLAHRDRIRRDLYREIVDRGY
                     FTRSPEVTRKLWRWIAWSGVAISVVFGIALTILTDAWALLPAIAGVIVSLVLVRVARR
                     MPQKTRKGAEAAARWRAFRTYLQDIRKYESVETATELFDRYLAYAVAFEIEKQWVKTF
                     ERAGTPAPRWYHSAGDILVLGEAGELLGEIGHLGRVPGPGDIGDVNLPDVGGIPNIGL
                     PDVGGVPNVDLPGAGDALQGASDLVSGGLQGASDGIFDLFDAAGSIFDGLDFDI"
     gene            complement(2879..4924)
                     /locus_tag="VGR22_01890"
     CDS             complement(2879..4924)
                     /locus_tag="VGR22_01890"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEV2127350.1"
                     /translation="MPVQIDDRLGRATWPRLLMLVAVALLAVGIPLPAGGQSDSVVWE
                     RYDVDLDVREDGTVHVTEDQTIRFDGTFTYGFADIPLDRVEGIDNLAITIDGEQARYV
                     EPSEYAEEPGTFTYRVSGDELNIDYAFEPTTFGEERNVTLEYDVSGAIRVYEDLEPAN
                     QQLWWTAISSDVTEVAPIRESTVTVTLPEDVPGEEVIAEPENPEVDGRTYAWTRTDLD
                     AGEDFEVRLQFPPITNAVVPAWQQLDDELREEALEQEGREAVAGTALFAAGLLILVGG
                     GIGFTLAWFARGRDPHVGAVADYIAEPPDDLSPGAAGTLVDETVQTRDVLATVLDLAS
                     KGAIAIHESGREEGLFGLGGGYRHELELKDPAKAERPFERRLLNLMFGLNADPGDRTS
                     LGEFRQAYAGNVESIADGFYDELVEHGYFHESPEATRKRWKGIAVGTPVALVIAAFVL
                     VNLFDVLMGWIAFVIVAAVIIAFYGAFLSAHMAVKTRQGAEAAAKWRAFRRYLEDIDE
                     RENLEESKEIFDRYLSYATAFGLEKSFVRKFAAAHVPMPGWFEGGGFGIPTAGGHPMR
                     RGRRTVVVGNPWFGGGYGGGTMGGGQATGGGRGDGGGGGLFGGFPDLQDTSDAGERTL
                     QSGSDSFFDMLDTAARAFSSGSRRSGRGSFGSFGGGRGFSGGGGFGGGGGGGGRGFG"
     gene            5120..5605
                     /locus_tag="VGR22_01895"
     CDS             5120..5605
                     /locus_tag="VGR22_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEV2127351.1"
                     /translation="MPQASAGQRATSISASRIRTILSQHGYRLTAPREAIVEQALRHD
                     RPFTAEQLVAELREHTDDVEAGIGRATVYRTLEILASVDVLSRVIQPNGNAAYMVDAP
                     GHRHYLVCSGCGTTVSFTSCPVETLVQHLTADTSFAIHDHLLEVFGTCPDCQRDAHAD
                     Y"
     gene            5787..6485
                     /locus_tag="VGR22_01900"
     CDS             5787..6485
                     /locus_tag="VGR22_01900"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV2127352.1"
                     /translation="MIDIMPGTITSILGPNGAGKSTLLKSLAGVIQPSHGSVRYRDRE
                     LKHPVPEITYIPQRSGAEWTFPISVLETVLLGLSRDTRRWRPFNTQERQQAIDALAHV
                     GLEPLTDVQIGALSGGQQQRVFLARALVACGEVLLLDEPFTGVDVPTQELFLDLFENL
                     RKRGVTIVFATHDLEHAARSSTRAILINNTVIADGPPAAILTDATLTNTFQGRIMVFR
                     EGGVPKVTEGPGQG"
     gene            6482..>6940
                     /locus_tag="VGR22_01905"
     CDS             6482..>6940
                     /locus_tag="VGR22_01905"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV2127353.1"
                     /translation="MIESLIAPWQSTFMQHAFAAIFIISVTSGIIGAFVVLRGIGFLG
                     DALAHAIFPGLAIAFITGGNILVGAFAAAVIVSLGIGAISQHRRINNDTAIGVLFVGA
                     FALGIALMTSQPTFQRDLTSFLFGSILGVTREDIGIIAVVSVLVLATLYYF"
BASE COUNT         1273 a         2435 c         2144 g         1088 t
ORIGIN      
        1 gatgaagaag ggtcgcccgg cgacgaaggt cagcctgctg gctccggcat ctcgccaggc
       61 acaactggaa cacgtcctga tgttcaacac tacgaccctc ggcacgcgct catggtcgat
      121 cgagcgcaca aaagcgggcc gagacttcgg tgccgtcacg acccgctggg gcgacgtccc
      181 cgtcaagctg cgcattctcg atgggcgggt gatcgacgcc cagcccgagt acgacgagat
      241 cgcccgtttg gcacgcgaat tcgaccagcc gatccgcgac gtctggaacg aggcgcatcg
      301 gctcggcgaa tcgttcgtcg ggcagccacg tcgctagcgc ggggcgatgc gccgggtggc
      361 cgggcgccga ccggtcacat gagttcgagc gaccgtacca tccataggcg tcacgggatc
      421 gcggtcaggc gacgggatga gccgtccagg cagggcgatc accgcgatgg cgacgtcgag
      481 agccattccc acccaggcgt cggcccagac ggcgccggca atcacgaacg agagcacggc
      541 ggtggcgagc accagcgacc gccaccagcg agcgccccgc cagagggcga ccgccccgac
      601 gatgaagctg accgcggcca ggagccacag cccgcccagc aggtcgacga cggccgtgga
      661 ggcgtccggc ggcgcgatct cctcgagatc ggcaatgccc cagaagtcga cggggcccat
      721 cagatgcacg gctccatgga gggcgagcag catggcaaac aggatgcgtc gcatgatggc
      781 tcctttcgcg aaccggttcg tcggcgcagc catacggtcc tgcaccgatc gtcttccagt
      841 ccccatcatg cgacagcaga cgtgacatcc gaatcacaga acgcccgcac gcgttacagg
      901 tttgcaacaa tcgattacgt caggacaaaa aaaccggttt tgccagactc cagcgacaga
      961 cgtcagcgaa accgcaccca gacgatcacc caggtcaggg ccatgaagag gatatcgggc
     1021 acgatacgga gctggacgaa gtcacgtggc atacccgcga gcagctgacc tccctcctgc
     1081 acgacgaccc gctcactgga gagcatcagg tcaatgggga tgccgtactt ctgggcgagg
     1141 agcaggcgca cggcgaccat cgcgagggtg acgatcgcga tccccagcac ctgcagatcg
     1201 gacccacgct tgcctttcgc gatccaggca atcgcctcgg cgacgccgaa gccgagcgcc
     1261 agcgagaggt agaacttcca gtccggagcg aggtaccaga gtgcagtcac caacgccgcg
     1321 acagccatcg ccagcccacc ggctctcgcc agcgcgttcg gggaggtgcg gatggtgggc
     1381 aggccccgta ccccggcgca atcggggcag cgcagtccga ccggcgtccg caccgcacac
     1441 cgcgggcaga tcggcttgcc gcaccgcgag cagcggagtc cggtcatgac gtctggatgg
     1501 tagctgcagg gcacctcgcc ggttgtgggt gcagcgaatt gctgggccat gagtctcctg
     1561 tggatcgtcg cgatgcacac gccgtcgctc acgcgttcgc cttacaggat accggtgagc
     1621 aggcgagaac ggctgtagtg ccggccccaa cgtgtatgag cacacgcccg gtcagatatc
     1681 gaagtcgaga ccgtcgaaga tggagccggc cgcgtcgaag aggtcgaaga taccatcgct
     1741 ggcgccctgc aacccgccgg aaaccaaatc cgaggcgccc tgcaaggcgt caccggcacc
     1801 gggcaggtcg acgttgggga caccgccgac atcgggcagg ccgatgttgg ggataccgcc
     1861 gacatccggc agattgacgt cgccaatgtc gcccgggccg ggcacccgcc caagatggcc
     1921 gatctcgccg agcagttcgc ccgcctcgcc gagtacgagg atgtcgcccg ccgagtggta
     1981 ccacctcggc gccggcgtcc ctgcccgctc gaacgtcttt acccattgct tctcgatctc
     2041 gaacgcgacc gcgtaggcca gataccgatc gaagagctct gtcgccgtct ccaccgactc
     2101 gtacttgcgg atatcttgaa ggtaggtcct gaacgcacgc cacctggcag ccgcctccgc
     2161 acccttccgc gtcttctgcg gcatccgccg cgcaacccga accagcacca gcgagacgat
     2221 cacgcccgcg atggccggca gcagtgccca ggcatcggta aggatggtca gcgcaatgcc
     2281 gaacaccacc gaaatggcca cgccactcca ggcgatccac ctccacagct tccgggtcac
     2341 ctcgggcgac cgcgtgaagt agccccgatc gactatctcg cggtagagat cccgtcgaat
     2401 tcggtctcgg tgagcgagaa acaccccccg gacgtcgctg aggcgaactt cctgcagtgc
     2461 ctgcgggtca ggaccgaaga gcacaacgag caagtcccgc tcgatgcgat cctcaatccg
     2521 ccggggatgg atcagcatca gcacgaagtc ccgcctgccg ttctcgctga cctgtgtgat
     2581 ctcaacggca ccgtgccggg cgagccccag cagggtcgcc atcacgtcat gttcatcggc
     2641 gtgctcgtcg atcagggtgc cgacggcacc gggcggaaga tcgtccggcg gctcggagag
     2701 gtactccgcg actggtccga tgtgcgggtc gcgcccgcgg gaataccaaa ggagcaggat
     2761 cgccagcagg ccgcccacga tgatgagcag catgccggcg agaaaaagca ggtggaggat
     2821 cgcggtcgca gtgtccattc agggggtcat ctcggtgaac ggcgaagcga gcggccggtc
     2881 atccgaatcc gcggccaccc ccaccaccgc caccgccaaa tccgccgcca ccgctgaagc
     2941 cacgtccgcc gccgaagctg ccgaaggagc cacgcccaga ccgccgcgag ccgctgctaa
     3001 aggcgcgcgc tgccgtgtcg agcatatcga agaagctgtc gctgccgctt tggagcgtgc
     3061 gctcaccggc atcactcgta tcctgcaggt ccgggaagcc gccgaaaagg ccaccgcccc
     3121 cgccgtcgcc acgaccacca cctgtggcct ggccaccacc catggttccg ccgccatagc
     3181 cgccaccgaa ccaggggttg ccgaccacca cggttcggcg accgcgccgc atcgggtgac
     3241 cgccagctgt cgggatgccg aagccgccac cctcgaacca gccaggcatc ggcacatggg
     3301 cggccgcgaa cttccggacg aacgacttct ccagcccgaa ggctgtcgcg taggacaggt
     3361 agcgatcgaa gatctccttc gactcctcca ggttctcgcg ctcgtcgatg tcctccagat
     3421 agcgacggaa ggcacgccac ttcgcagccg cctcggcacc ctgccgggtc ttgacggcca
     3481 tgtgcgccga gaggaacgcg ccgtagaagg cgatgatcac cgctgcgacg atcacgaaag
     3541 caatccagcc catcaagacg tcgaacaggt tcaccagcac aaacgcggcg atcaccagcg
     3601 cgaccggcgt gccgaccgcg atgcccttcc accgctttcg cgttgcctcc ggtgactcgt
     3661 gaaagtagcc gtgctcgacc agctcgtcgt agaacccgtc ggcgatcgac tcgacgttac
     3721 ccgcgtaggc ctgccgaaac tctccgaggc tggtgcggtc gcccggatcg gcattcaggc
     3781 cgaacatcag attgagtagc cgccgctcga acggtcgttc cgccttcgcg ggatccttca
     3841 actcgagctc gtgtcggtag ccgcccccga gtccgaagag cccctcctcc ctgcccgact
     3901 cgtgaatggc gatcgctccc ttgctcgcga gatcgaggac tgtggcaagg acatcacgcg
     3961 tctgcaccgt ctcatcgacc agcgtgccgg cggcccccgg tgagagatcg tccggcggct
     4021 cggcgatgta gtcggccacc gcgccgacgt gcgggtcacg tccgcgcgca aaccacgcca
     4081 gcgtgaagcc gatccccccg ccgacgagga tgagcagccc ggcggcgaag agcgccgtgc
     4141 ccgcgacggc ctcgcgcccc tcctgctcga gggcctcctc gcgcagctcg tcatcgagtt
     4201 gctgccatgc cggcaccacc gcatttgtga tcggcgggaa ctgaagccgc acctcgaagt
     4261 cctcgccggc atcgaggtct gtgcgtgtcc aagcgtaggt gcggccatcg acctccgggt
     4321 tttccggctc cgcgatcacc tcctcccccg ggacatcctc gggcagcgtg acggtgacgg
     4381 tcgactcgcg tatcggtgcg acctcggtga catcggagct gatcgctgtc caccagagct
     4441 gctggttggc cggctcgaga tcctcgtaga cacggatcgc gccgctgacg tcgtactcaa
     4501 gcgtgacatt gcgctcctcg ccgaacgtcg tcggctcgaa cgcgtagtcg atattcagct
     4561 catcgccaga gacgcgatag gtgaaggtgc cgggctcttc ggcgtattcg gacggctcga
     4621 cgtagcgcgc ctgctcgccg tcaatggtga tggcgagatt gtcgatgccc tcgacccgat
     4681 ccaaggggat gtccgcgaaa ccgtacgtga aggtaccgtc gaagcggatg gtctggtcct
     4741 cggtcacatg gactgtgccg tcctcgcgca catcgaggtc gacatcgtag cgctcccaga
     4801 ccacggaatc cgactgccca ccagccggga gtggtatccc gacggcgagc agcgcgacgg
     4861 ccaccagcat cagcagccgg ggccacgtcg cacgtcccaa gcggtcgtca atctgcacag
     4921 gcatgcgccc gtatcctctc tctcatcgat tgcattgccg ggcgtccgcc cgcgatgggg
     4981 tcccaataac ggccttcccg actacctgac ggcaccgctg caggcttccg tgaggtattc
     5041 cgagtaccat tgtacgaggt tcgttagcgc tgccgaggca tgattccggc actccaacgc
     5101 gcacccgagg aggactttag tgccacaggc cagcgccgga cagcgcgcga cgtcgatcag
     5161 cgcctcgcgc atccgcacca tcctgagcca gcacggctac cggctgaccg cccctcggga
     5221 ggcgatcgtc gagcaggcgc tccggcacga ccgccccttc actgccgagc agcttgtggc
     5281 ggagctgcgt gagcacacgg acgacgtcga ggccggcatc gggcgagcga cggtctaccg
     5341 cacactcgaa atcctcgcct cggtcgacgt gctctcacgc gtcattcagc ccaacggcaa
     5401 cgccgcctac atggttgacg cgccgggaca ccggcattac ctcgtctgct ccggctgcgg
     5461 caccacggtg tcgttcacga gctgcccggt cgagacgctg gtgcagcacc tcaccgccga
     5521 caccagcttc gccatccacg accacctcct ggaggtcttt ggcacctgcc cggactgcca
     5581 gcgcgacgcg cacgctgatt attgacacta tgtctcaaaa cgtgtagggt gacagacgcg
     5641 ccgaatgcgg ccctacccaa cgttgggcgt tcctggagtt ccccaccgtg cgtccctgcc
     5701 cccctgcacc cgaccctgat cgcccggtgc tgagcacgcg gaatctgagc gttcacttcg
     5761 gctcccgatc ggcgatcgat caggttatga tcgacatcat gcccggcacg atcaccagca
     5821 ttctcggccc caatggcgcc gggaagtcca cgttgctcaa gagcctggcc ggcgtgatcc
     5881 agccgtcgca cggatcggtc cgataccgcg accgcgaact caagcacccc gtgccggaaa
     5941 tcacgtacat cccgcagcgc agtggggccg agtggacatt cccgatctcg gtactggaga
     6001 ccgtgctgct gggtctctcg cgggataccc gccgttggcg gcccttcaat acacaggagc
     6061 ggcaacaggc gatcgatgcg ctggcccatg tcgggctgga accgctcacc gacgtgcaga
     6121 tcggcgcgct ctccggtggc cagcaacagc gcgtgttcct ggcacgggcg ctggtagcct
     6181 gcggcgaggt gttgctgctc gacgagccct tcaccggcgt ggatgtcccc acccaggagc
     6241 tcttcctcga cctcttcgag aacctgcgga agcgcggcgt gacgatcgtc ttcgcgacgc
     6301 acgatctcga gcacgccgcg cggtcgtcca cacgcgcgat cctgatcaac aacacggtga
     6361 tcgcggacgg accaccggcc gcgatcctga ccgacgcgac gctcaccaat accttccaag
     6421 gacggatcat ggtcttccgg gagggtggcg taccgaaagt cacggaagga cctggccagg
     6481 gatgatcgag agcctgatcg cgccgtggca gagtacgttc atgcagcacg ccttcgccgc
     6541 gatcttcatc atcagtgtga ccagcggcat catcggagct tttgtcgtgc tgcgcggcat
     6601 cgggttcctc ggcgatgccc tcgcccacgc gatcttcccc ggcctggcga tcgccttcat
     6661 caccggcggc aacatcctgg tcggcgcctt cgccgccgcg gttatcgtct cgctcgggat
     6721 cggcgcaatc agccaacacc ggcgcatcaa caacgacact gccatcggcg tgctgttcgt
     6781 cggcgcgttc gcgctcggca tcgcgctgat gaccagccag ccgacgttcc agcgcgatct
     6841 caccagcttc ctgttcggat cgatccttgg ggtcacccgc gaagacatcg gcatcatcgc
     6901 cgtcgtctcg gtgctggtac tggcgacgct ctactacttc
//
LOCUS       DASYWL010000056        14594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_396731, whole genome shotgun sequence.
ACCESSION   DASYWL010000056 DASYWL010000000
VERSION     DASYWL010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 14594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14594
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396731"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VGR22_01910"
     CDS             <1..185
                     /locus_tag="VGR22_01910"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV2127354.1"
                     /translation="IGPRIGLGVFLVTIIPGQLIGGLLLDHVGAFGSLPRQIDPIRIA
                     GALVLILGVVLIRGRP"
     gene            complement(262..1782)
                     /locus_tag="VGR22_01915"
     CDS             complement(262..1782)
                     /locus_tag="VGR22_01915"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEV2127355.1"
                     /translation="MTSESVTKLREHLATVSDLGGAASLLGWDQRTKMPTAGAAVRGE
                     RLATLGRLAHELFVSDMTAKLLEAAEKELDGLDSDSDEASLVRHTRREFDRSAVISSD
                     LMTESIRASSDGYGRWVEARADSDFASFRPALERILEVNRRFVEAYRTIQPDAAEDYD
                     LLLEEFEPGLTSAEISEAFDVVRKRTIPLVDRVREHADKVDDSLVHRAFPPADQEHLA
                     LKVIRQCGFDDKHWRLDETHHPFASSMATTDVRITTRYMPEFFNAAFFGSMHEFGHGL
                     YERQVSPSLERTPLARGTSMAWHESQSRMWENLVGRSRAFWDWAITPVREAFPETLGS
                     ASAEDMYRAVNKLHPSLIRVEADELTYNLHIILRFELEREMLAGSVELRHLPEAWNQK
                     MCDYLGVEVPDDAHGVLQDVHWSSGLFGYFPTYALGNILSLQLWQRITADLPDLEERI
                     RVGEFAPLREWLGENIHVHGSKFRPKDLMEKVLGTRKLDPGPLTNYLEAKVDDLYS"
     gene            complement(1785..2528)
                     /gene="purQ"
                     /locus_tag="VGR22_01920"
     CDS             complement(1785..2528)
                     /gene="purQ"
                     /locus_tag="VGR22_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEV2127356.1"
                     /translation="MSNTPRVAVIGFPGSNGDHDALHALAHDVGVNAELIDYRATALN
                     GIDAVVLPGGFSYGDALRAGAIARFAPIMGAVQEFARSGGPVLGICNGFQVLTEAHLL
                     PGALLRNRTLKFHCHVTHVRLEDAESIWLSNVSPGEVLSLPIAHGEGSYYVDDETLAR
                     LEDRNQIAFRYVEKDGSLTDEANLNGSVSAIAGVISEAGNVLGLMPHPERATDPLTSN
                     GSGLKILSSLLGSGDGRQVAGSNRHNAVR"
     gene            complement(2528..2800)
                     /gene="purS"
                     /locus_tag="VGR22_01925"
     CDS             complement(2528..2800)
                     /gene="purS"
                     /locus_tag="VGR22_01925"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF004630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEV2127357.1"
                     /translation="MRRWTVKAIIMPKHGVNDPQGEAIKGGLQSLEFSGIEDVRAGKQ
                     IAIMLEARSREEAVAAVERMCEQLLANPVIETYSISADEARQESAT"
     gene            complement(2797..3708)
                     /locus_tag="VGR22_01930"
     CDS             complement(2797..3708)
                     /locus_tag="VGR22_01930"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEV2127358.1"
                     /translation="MTDTPPVLDMELTHYPKWRSGKVREIFDLGDAFLFVATDRISAF
                     DVILPDAIPGKGVLLTQVSRFWFEQTRHIVSNHVLDFDLDDLDLSDEERAMLQGRAMV
                     VRKAERIDVECVVRARLAGSGWEEYRQHATLAGAPLPVGLRPGDRLPELRFTPATKND
                     AAHDDNISTADLRAAVGRNLAELLEVSSKAIFRHAEGIATHAGFAIADTKFEFGFIDG
                     ELSLIDEVLTPDSSRYWDLEELAPGSAPQGYDKQVIRNWLLESGWDREPPAPSLPPEI
                     IKIARGRYADVLRRLRSAATSEPGATS"
     gene            complement(3705..5018)
                     /gene="purB"
                     /locus_tag="VGR22_01935"
     CDS             complement(3705..5018)
                     /gene="purB"
                     /locus_tag="VGR22_01935"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEV2127359.1"
                     /translation="MIDRYTLPEMGGIWNEQAKIDRWLDVEKAVCEAWAARDRIPGDA
                     MARIRKAACDLDRMKAIERETDHDVIAFLRATGETVGEASRFIHLGLTSSDVVDTGLA
                     MQARDAGRLLQRRLAILVETVGRQAVKHKRTLTIGRSHGMHAEPTTLGMKLAVWYDEL
                     RRHQQRLEMATDHIAAGQISGAVGTHAHVAPDLEEEVLERLGLGVELASTQVAQRDRH
                     AFFLAVLAGIGATLEKFAVEIRHLQRSEVAEAEEFFEEGNQGSSAMPHKRNPHESERL
                     AGLARLLRGYALAGNENVALWHERDISHSSVERVIFPDSCIVLDYMLHLANELIGEWV
                     IHPDRMRANLEMSGGLIYSQRVMLALVEAGMDRQQAYKIVQSEAMAASKSPRGPSFRE
                     RIEQHPEVRRAASPEQIGAIFDPWDQLHSIGATFRRLGLESEVPV"
     gene            5146..5925
                     /locus_tag="VGR22_01940"
     CDS             5146..5925
                     /locus_tag="VGR22_01940"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEV2127360.1"
                     /translation="MKFVDTHIHLDSDAFDEDRDRVIAASRSAGVTRWINVGYNEQRW
                     ETTEELVRTVNGMYCMLGLHPGDADAWSDDMLPRLRGRVDAVRPLAIGETGIDLHWRQ
                     DNLDIQRAAFRGQLALAGDAELPAVIHMRDADVELLDVLTSENSLPHIHLHSFDGNDM
                     LRQWVLETDATVGVGGLITRKGSESLRQWVGNLPHDRVVLETDAPYLKPRRIRGKRNE
                     PAYIVAVAKLLSELWGTDLASVAATTTRNAERIFALKEEKA"
     gene            5925..6521
                     /gene="rdgB"
                     /locus_tag="VGR22_01945"
     CDS             5925..6521
                     /gene="rdgB"
                     /locus_tag="VGR22_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEV2127361.1"
                     /translation="MPRLLIATGNTGKIAEMEALIGDRAEVISLAEAGIESPEETGTT
                     FEENAALKAVNAARESGLVTVADDSGIEVDALGGAPGVRSVRYAGDDATDEQNIAKLL
                     HELEGVPDEARTAGFVSVIVVATPDGRARAFRGTLEGMVGRSPRGGGGFGYDPVMVLG
                     DGRTVAELEPGEKNGISHRGTALRNALPYIHELLDSGA"
     gene            6540..7772
                     /locus_tag="VGR22_01950"
     CDS             6540..7772
                     /locus_tag="VGR22_01950"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEV2127362.1"
                     /translation="MIAAYSPLTELRDLPPSFRHWQKRCVEYGLPPVIAVAGSRGKST
                     VVRMLQAVFDLAGVRSAIWTDFGVEINGRRQSREIAGWNRALARLTERTLDVAVQELD
                     WNLISAVGLPHATYPIGIITNLCSNSTQCRHTAAGRIALRALPRVIDAVHSDGFICLN
                     GEDYDLERAVQGESAHPVVVAKSDGAPMLRQQRDAGGIHLWLKPDGSVVWGNADDRHE
                     IVNVHHVPVCLDGAASFEQTNVMLASAAGLATGIGTEIIRQALTNFTLSVDQLPGSFS
                     ARDIGRIHTVVDRMMPSWFLKPVLRAANPHARRRQITVLSGLDRLPADDVFEVGRILG
                     RYSGAVIHHGETDRARFEAFRRGIARNDYPPVLVSLPTERRAINRAMRAVRAEDVALF
                     LCTDDPGPAIRAIDRMKT"
     gene            complement(7769..11245)
                     /locus_tag="VGR22_01955"
     CDS             complement(7769..11245)
                     /locus_tag="VGR22_01955"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEV2127363.1"
                     /translation="MAEFAHLHLHTEFSLLDGVGRIDQYIERAHQLGLQHIAVTDHGV
                     MYASLSWYNAATEAGLHPIIGMEAYLAEGLISERKRESYHLLLLAANETGYRNLLKLA
                     SIASLEGFYYRPRIDLELLQQYHEGIIATSACIGGPVAKPFLNGDIERAERYAGTLSE
                     IFGRDRFFIEIQNHGIDLEEEANRFMIPLAKRMNLPIVATNDVHYNEEDDAAAQDLLV
                     CVQTNTTLNDPKRLKSDSTQLYLKSAGEMERLFGHIPEAITNTVRIAEMCSLDLGFKG
                     YQLPHFDVPEEFTPSAYLRKLVEEGARRKYGTIEGAVCERIDHELGIIENMGFTNYFL
                     IVWDFVRFAKERGILVGPGRGSAAGSVVTYALDITGLDPLKYELFFERFLNPSRISMP
                     DIDIDFADDRRDEVIEYVVDKYGDDRVAQIITFGTLKAKAAVRDVGRAMDLGFGETDR
                     VARLIPTDPKMTIEKALETVPDLGKLYESDAQVRELIDTAKKVEGHARHSSTHAAGVV
                     ISRDPLVEQVPLQYAGGKSEGDVTTQWTQDHLESLGLLKMDFLGLKTLTVLGKAIALL
                     AESGHNISLEDTPLEDPEALALLRRGETFGVFQLEGGMTRRMTIDVAPESFDDLVALM
                     ALIRPGPLELAPDFIARKHGREPVEYIHPLVEPILEKTYGIAIYQEQVMQIANAVAGF
                     SMAEADGLRKAMGKKLPEVMAKYESRFIEGAVENGTDKDLAKQIFDMIEAFAGYGFNK
                     AHSAAYAVIAAQTGYFKANFPVQFMAALMSTEMNSSEKTVVNIAECRRTGIPILPPDV
                     NRSGVEFSVEKLDDGTEAIRFAMGAIRNVGVGAVETIVAARKEQPTGEFASLEDFCDS
                     VSWSKVGRRVAENLCKVGALEGFGERNAVLKALETAIASAQQRQKAADRGQTDMFGMV
                     TAASPGPSERTLSQVPEASNRERLDWEKELLGFYLSAHPINEVVGKHIPQGYAEIVDL
                     ESREPGERVRMLGMVVDVRKIITKTNKSMAIVKIEDLTGTIEVVCFPETYERIGEMLE
                     VDAIIEVSGKFERRNEEPQIVADNATRDLPEFEAGRPKDEPIVALQLPGSEDVWSDIS
                     LMQNIDRVLNNHEGTIPVEFRLPRGTRVIRLRSRSRTVEWSDEFRGEMGELLGATAVT
                     LEEPARESQAAD"
     gene            complement(11428..11853)
                     /locus_tag="VGR22_01960"
     CDS             complement(11428..11853)
                     /locus_tag="VGR22_01960"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV2127364.1"
                     /translation="MAVQVREAGSAFGRVLKEFREQRRVSQSKLAKRAGFDHSYVSRL
                     ESGARTPTREAVEQLAGALELDGVQQDELLVSAGFLPREVSSLLAGEPEITEVLNLLQ
                     NAEVPEEYRSSMRQVLRVLADQARLVLKGEKAAGAGFAA"
     gene            12237..13211
                     /locus_tag="VGR22_01965"
     CDS             12237..13211
                     /locus_tag="VGR22_01965"
                     /inference="COORDINATES: protein motif:HMM:NF014881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HEV2127365.1"
                     /translation="MPADGPEPVETYVLKGSRLAKRLRAEIAASQQHLLDVGGEPPRL
                     ATLIVGEDRAAIAYRSSIERTFARAEIDVLRAELPGDVSEQTFLEELRELNRDPQVTA
                     VLVLMPLPAHLNPHLVLENLSPLKDVDGITPTNAGRLHLGLPSLRPSTPAGGLALLDH
                     YGVTLEGARVTVVGRSNVVGRPLATLLTQRDATVTLCHSKTVDLDRETRRAEIVCVAA
                     GHAGLISRDMVSRGTVVIDFGVNVTDEGKLTGDADFDGLMGIAGAITPVPGGTGPVTA
                     LTLAQNTMAAGFAALGGNLDYVDYALPDRLQQKINDATREGVEQPTAS"
     gene            13231..13304
                     /locus_tag="VGR22_01970"
     tRNA            13231..13304
                     /locus_tag="VGR22_01970"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13263..13265,aa:Gln,seq:ctg)
     gene            complement(13850..>14594)
                     /locus_tag="VGR22_01975"
     CDS             complement(13850..>14594)
                     /locus_tag="VGR22_01975"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEV2127366.1"
                     /translation="AGMIAASWFVDPAWATAKATVLLGAVLPEGQETIEAIVEDAIAQ
                     RGRTGVLALAVLLVTGRRVLGAVTKALNLVSDVDERDDPLERRAAVEVSLITGLIGAL
                     LLAFASRPLLELLWGMIGILPGRDGALLPVAHQLVRAGLLVVVFTLVYVVVPRGERFW
                     RAAFAGAVVATALFLGARGVFVVLFLFDVLWPNLTLIYGPLAVAALLLSWGWYIALII
                     LAGGALASHVKVMILEGRPAEEAGDAHVR"
BASE COUNT         2580 a         4804 c         4546 g         2664 t
ORIGIN      
        1 tcatcggtcc gcggatcggg ctcggcgtct ttctcgtcac gatcatcccg ggccagctca
       61 taggcggctt gctgctggac catgtcggcg ctttcggctc cctaccacgg cagatcgacc
      121 caatccggat cgccggcgcc ctggtcctga tcctcggcgt cgtcctcatt cgcggccgac
      181 cataaccttc ctactgcggc gcattgcggc ccttgtgcag gttgcacaag ggccgctcgt
      241 cgtgtctggg tggcggcgct gtcagctgta gagatcgtcg accttggctt cgagatagtt
      301 cgtgagcggt cccggatcga gcttgcgcgt gccgagcact ttctccatca gatccttcgg
      361 cctgaacttg ctgccatgca catggatgtt ctctccgagc cactcgcgca gcggcgcgaa
      421 ctccccgaca cggatccgct cctcgaggtc cgggaggtcg gcggtgatgc gctgccacag
      481 ctgcagcgag aggatgttgc ccagcgcata ggtcgggaag tacccgaaga gccccgagct
      541 ccagtgaacg tcctgcagca cgccatgggc gtcgtcaggc acctccacgc cgaggtagtc
      601 gcacatcttc tggttccacg cctccggcaa gtgcctgagc tcgacgctac cagccagcat
      661 ctcccgctcc agctcgaacc gaaggatgat gtgcaggttg taggtcagct catcggcctc
      721 tacacggatc agggatgggt gcagcttgtt caccgcgcga tacatgtcct ccgcgctggc
      781 gctgccgagg gtttcgggga acgcctctcg taccggcgta atcgcccagt cccagaaggc
      841 gcggctgcga cccaccaggt tctcccacat ccggctctgc gactcgtgcc atgccatcga
      901 ggtgccgcgc gcgagtggcg tgcgctccag cgacgggctt acctgccgct catacagccc
      961 gtgaccgaac tcgtgcatgc taccgaagaa ggcggcattg aagaattccg gcatgtatcg
     1021 cgtggtaatg cggacatcgg tcgtcgccat cgaagaagca aatgggtgat gcgtctcatc
     1081 gaggcgccaa tgcttgtcgt cgaagccgca ctgccggatc accttgagcg ccagatgctc
     1141 ctgatctgcg ggagggaatg cgcgatgtac gagagaatcg tcgaccttgt cggcatgctc
     1201 gcggacgcgg tcgaccagcg ggatggtgcg cttcctgacg acgtcgaagg cttcggagat
     1261 ctcggcggag gtgaggccgg gctcgaattc ctccaggagc aggtcatagt cttcggccgc
     1321 atccggctgg atggtgcgat aggcctcgac gaatctgcgg ttcacctcca ggatgcgctc
     1381 gagcgccggc cggaagctcg cgaagtcgga gtccgcgcga gcctctaccc acctgccgta
     1441 gccgtccgat gacgcccgga tggactcggt catcaggtcc gaggagatga ccgcggatcg
     1501 gtcgaactct cgccgagtgt gacggaccaa agaggcctcg tcactgtcgg agtcgagccc
     1561 atccagctcc ttctccgcgg cctccagcag cttcgctgtc atgtcggaga cgaacagctc
     1621 gtgggccaga cgacccagcg tcgcgagccg ctcaccgcgg accgccgcgc ccgcggtcgg
     1681 catcttggtg cgctggtccc agcccagcag gctcgccgca ccaccaaggt cgctcacggt
     1741 tgcgaggtgc tctcgaagtt tagttacaga ctcggaagtc atggttacct cactgcgttg
     1801 tgccggttcg acccggcgac ctggcggccg tcgccagagc ctagcaggct cgacaggatc
     1861 ttcaggccgc tgccgttgct cgtgagcggg tcggttgctc tctccgggtg cggcatcagg
     1921 ccgagcacat tgccagcctc gctgatcacg ccggcgatag cactcacgga tccgttcagg
     1981 ttcgcctcgt ctgtgaggga gccatccttc tcgacgtacc gaaaggcgat ctgattcctg
     2041 tcctccagcc gggcgagagt ctcatcgtcg acgtagtacg acccctcgcc gtgcgcgatc
     2101 ggcagcgaga gcacctcgcc gggcgagacg ttgctcagcc agatcgactc ggcatcctcg
     2161 agccggacat gggtgacgtg gcagtggaac ttgagcgtgc gattgcgaag gagcgcgccg
     2221 ggcagcaggt gcgcctcggt caacacttga aagccgttgc agatgcccag cacaggccca
     2281 cctgagcggg cgaactcctg cactgcgccc atgatcggcg cgaaccgcgc gatggcgccg
     2341 gcgcgaagcg catcgccgta ggagaagccg cccggcagca cgacggcatc gatgccattc
     2401 agcgccgtgg cccgatagtc gatgagctcg gcgttgacgc cgacatcgtg cgcgagcgcg
     2461 tgcagtgcgt cgtggtcgcc gttgcttccc ggaaacccga tgacggcgac gcgcggggtg
     2521 ttagacatca cgtcgcactc tcctgccggg cctcgtccgc actgatgctg taggtttcaa
     2581 tcaccggatt ggcaagcagt tgctcgcaca ttcgctcaac cgccgcgacg gcctcctcac
     2641 gggatcgcgc ctccagcatg atggcgatct gcttacccgc acgaacgtcc tcgatgccgc
     2701 tgaactcgag gctctgaagc ccccccttga tggcctcgcc ctgcgggtca ttgacgccgt
     2761 gcttgggcat gatgattgcc ttgaccgtcc accttctcac gatgtcgctc ctggttcgga
     2821 cgtcgctgcg gaccgcagcc gacggagaac gtcggcatac cggccccgag cgattttgat
     2881 gatctccggc ggcaacgagg gcgcaggcgg ctcgcggtcc cagccgctct ccagcagcca
     2941 gttgcggatc acctgcttgt cgtacccttg gggcgccgat ccgggcgcga gctcctccag
     3001 atcccagtac cgcgatgaat ccggcgtcag cacttcgtcg atcagcgaga gctcgccgtc
     3061 gatgaagccg aactcgaact tggtgtccgc gatggcgaaa ccggcatgcg ttgcaatgcc
     3121 ttccgcgtgc cgaaagatcg ccttgctgct cacctccagc aactctgcaa ggttcctgcc
     3181 gaccgccgcg cgcaagtctg cggtcgagat gttgtcgtcg tgcgccgcat cgttcttggt
     3241 ggctggagtg aagcgcagct ccggcaaccg atcgcccggt cgcaacccca ccggcagtgg
     3301 cgccccggcg agcgtagcgt gctgtcgata ctcctcccag ccggagccgg cgagccgtgc
     3361 gcgcaccaca cactcgacgt cgatccgctc ggccttccgg acgaccatcg cgcgcccttg
     3421 cagcatcgcg cgttcctcgt cactgaggtc gaggtcgtcc aaatcgaagt cgagcacatg
     3481 attggacacg atgtgccgcg tctgctcgaa ccagaatcgg ctcacctgcg tcagcaacac
     3541 gcccttgccg ggaatcgcat cgggcagaat gacatcgaac gcggaaatcc ggtccgtcgc
     3601 aacaaaaagg aaggcgtcgc cgagatcgaa gatttcgcgg accttgccac tgcgccattt
     3661 tgggtagtgc gtgagttcca tgtcgagaac ggggggtgtg tccgtcacac cggcacctcg
     3721 ctctccagcc cgaggcgccg aaacgtcgcg ccgatgctgt gcagttggtc ccaaggatcg
     3781 aagatcgcgc cgatctgctc cggagacgcc gcgcgccgga cctcaggatg ctgctcgatc
     3841 cgttcccgga atgacggacc cctaggactc ttcgacgctg ccatcgcctc ggactgtacg
     3901 atcttgtacg cctgctggcg gtccatgccc gcttcaacaa gcgcgagcat cacgcgctgc
     3961 gagtagatca ggccgccgct catctccagg ttcgctcgca tgcgatcggg gtggatcacc
     4021 cactcgccaa tcaactcgtt ggcgaggtga agcatgtagt cgagcacgat gcaggaatcg
     4081 ggaaagatca cccgttcgac cgagctgtgg ctgatgtcgc gctcgtgcca gagcgcaaca
     4141 ttctcgttcc cggccagcgc ataaccgcgc agcagccgtg ccagcccggc gagccgctcc
     4201 gattcgtgcg ggttgcgctt gtgcggcatc gccgaggagc cctggttgcc ctcctcgaag
     4261 aactcctcgg cctcggcgac ctccgagcgt tgcaggtgcc ggatctcgac tgcgaacttc
     4321 tcgagcgtcg caccgattcc cgccagaaca gcgaggaaga acgcgtggcg gtcgcgctgt
     4381 gcgacctgcg tcgacgccaa ctccacgccg agtccgagcc gttccagcac ctcctcctca
     4441 aggtcgggag cgacatgggc gtgggtgccg actgcaccgc tgatctggcc cgcggcaata
     4501 tggtcagtag ccatctcgag gcgctgctgg tgtcggcgaa gctcgtcgta ccagacggca
     4561 agtttcatgc cgagggtcgt tggctcggca tgcatgccat gagaccgacc gatggtcagt
     4621 gtacgcttgt gctttactgc ctgccgcccg accgtctcga caaggattgc gagtcgtcgc
     4681 tgcaggagac gcccggcatc tcgcgcctgc atggcaagac cggtatcgac aacatccgag
     4741 gatgtcaggc ccaggtggat gaaccgcgac gcctcgccga ccgtctcgcc ggtggctctg
     4801 aggaaggcga tcacatcgtg gtcggtctcc ctctcgatcg ccttcatccg gtcgagatca
     4861 caggctgcct tccgaatgcg ggccatggcg tcgcctggaa ttcgatcccg agctgcccaa
     4921 gcctcgcaca ccgccttctc cacgtcgagc cagcgatcga tcttcgcctg ctcgttccag
     4981 atcccgccca tttccggcag cgtgtatcga tcgatcacgc gtctcgctcc attctgtgct
     5041 ggggatggcc gcccaaatgg cgccgttgcc atggtcagta tacggtggta tcgggttcac
     5101 tacggtctcg gtgtggggcg ggacgcgcga ctgaatggaa tacacatgaa atttgtcgac
     5161 acccatattc atctggacag cgacgcattt gatgaagatc gcgaccgcgt gatcgcggcg
     5221 agccgctcag cgggtgtcac ccgctggatc aacgtcggat acaacgagca gcgctgggaa
     5281 accacggaag agctggtgcg gaccgtaaac ggtatgtact gcatgcttgg cctccacccc
     5341 ggcgacgccg acgcctggag cgacgacatg ctcccccgcc tccgcgggag agttgacgcg
     5401 gtacggccgc tggcgatcgg cgaaaccggc atcgacctcc actggcggca ggataatctc
     5461 gatatccagc gggcagcgtt tcggggacag ctcgcgctcg ccggtgatgc cgagctaccg
     5521 gcggtgattc acatgcgcga cgccgacgtg gaattgttgg acgtgctgac gagcgagaat
     5581 tcgctgcctc acatccatct ccacagcttt gacggcaacg atatgctccg ccagtgggtc
     5641 ctcgaaacgg acgcgacagt gggtgtcggc gggctcatca cccgcaaggg ttcggagagc
     5701 ctgcggcagt gggtgggcaa tctccctcac gaccgggtcg tgctcgaaac cgacgccccg
     5761 tacctcaagc cccgtcgaat tcgaggcaag cgcaacgagc ccgcatacat cgtcgcggtg
     5821 gcgaagttgt tgtcggaact ctggggcacg gaccttgcat ctgtagcagc gacgacaacc
     5881 cgaaacgcgg agcggatctt cgcgctgaag gaggagaagg cgtgatgcca cgtctgctga
     5941 tcgccacggg caacacgggc aagatcgccg agatggaggc gctgattggc gatcgcgccg
     6001 aagtgatctc gcttgccgaa gccggcattg agagtcccga agagacaggt accacgtttg
     6061 aggagaatgc agccctgaag gctgtgaacg cggctcggga atcaggactg gtgacagtcg
     6121 ccgatgactc cggcatcgag gtcgatgcgc tcggcggggc acccggggtg cggtctgtcc
     6181 gctatgccgg tgacgacgcg actgacgaac aaaacatcgc aaagctgctc cacgagctgg
     6241 aaggggtgcc cgacgaggct cgaacggccg ggttcgtctc ggtgatcgtc gtcgcgaccc
     6301 cggacgggcg cgcgcgggca ttccgtggca cgctggaggg catggtgggc cgatcgccgc
     6361 gaggcggcgg ggggttcggc tacgacccag tcatggtgct tggcgatggg cgcactgttg
     6421 ctgagctgga accgggagaa aagaacggaa tcagccaccg cggcacagcg ctcagaaacg
     6481 cgctacccta catacatgag ttattggact ctggggcctg atggccggaa ggatgtcccg
     6541 tgatcgcggc gtacagccca ttgaccgagt tacgggatct gccaccgtcg tttcgtcact
     6601 ggcaaaagcg atgcgttgaa tacggcttgc cgcccgtcat cgccgtggca ggatcacgcg
     6661 gcaagtcgac cgtcgtgcgc atgcttcagg ccgtgttcga tcttgccggc gtgcgaagcg
     6721 cgatctggac ggacttcggc gtcgaaatca acggacggcg gcagtcccgc gagatcgccg
     6781 gctggaaccg tgcgctggcg cgtctgaccg agcgtactct cgacgtggcg gtgcaggagc
     6841 tggactggaa cctgatatcg gccgtcggcc tccctcatgc cacctatccg atcggcatca
     6901 tcacgaacct ctgcagcaac agcacgcaat gccggcacac ggctgcgggc cggatcgcgc
     6961 tgagggcgct tccccgcgtg atagatgcgg tccactcgga cggattcatc tgcctcaacg
     7021 gcgaggacta cgatcttgag cgagccgtgc agggggaaag cgcgcaccca gtagtggtcg
     7081 caaagtcgga tggtgccccc atgctccgcc agcagcgcga tgccggtggg attcacctct
     7141 ggctaaagcc ggacggctcg gtcgtctggg gaaatgccga tgatcgccat gaaatcgtga
     7201 acgtgcacca tgtgccagtt tgtctggacg gcgcggcctc gttcgaacag acgaacgtca
     7261 tgctggcctc tgcagcgggg ctggccacag gcatcggaac cgagatcatc cgccaggcgc
     7321 tgaccaactt cacattatcc gtcgatcagc tgccgggctc gttttcggcg cgggacatcg
     7381 gtcgaatcca cacggtagtt gatcggatga tgccctcgtg gttcctgaag ccggtgctgc
     7441 gtgcggcgaa cccgcatgca cgtcgtcggc agatcacggt gctgagcggc cttgatcggc
     7501 tacctgccga cgacgtcttc gaggtgggcc ggatcctcgg tcgatactcc ggtgcggtca
     7561 ttcatcacgg cgagaccgat cgggctcgat tcgaagcatt tcggcgaggg atagcgagaa
     7621 acgattatcc accggttctg gtcagcctgc cgaccgaacg gcgcgcgatt aaccgcgcca
     7681 tgcgagcggt gcgagccgag gatgtcgcgc tcttcctctg caccgacgat ccgggtccgg
     7741 ctatccgcgc aatcgatcgc atgaaaacct agtccgccgc ttgggactcg cgcgccggct
     7801 cctccagcgt caccgccgtc gcaccgagta gctcgcccat ctcgccccgg aactcgtcgc
     7861 tccactcaac cgtccggctc cggctgcgaa gacggataac gcgcgttccc cgcggcaacc
     7921 ggaactccac ggggatcgtt ccctcgtggt tgttgagtac gcggtcgatg ttttgcatca
     7981 aggatatgtc gctccaaaca tcctcgctgc cgggcagctg cagcgccacg atcggctcgt
     8041 cctttgggcg tcccgcttcg aactccggca ggtcgcgggt tgcgttgtcc gccacgatct
     8101 gcggctcctc attgcgccgc tcgaactttc cggatacctc gatgatcgcg tccacctcga
     8161 gcatctcgcc gatccgctcg tacgtctccg ggaagcacac cacttcgatc gtgccggtga
     8221 ggtcctcgat cttcacgatc gccatggact tgttcgtctt cgtgatgatc ttccgcacgt
     8281 ccaccaccat gccgagcata cggacccgtt cgccgggctc ccgggactcc aggtcgacga
     8341 tctccgcgta gccctgcggg atgtgcttgc cgacgacttc gttgatcgga tgtgcgctca
     8401 gatagaatcc aagcagctcc ttttcccagt cgagacgctc tctgttgctt gcttccggca
     8461 cttgggacaa cgtcctctcg cttggtccgg gtgacgcggc agtcaccatg ccgaacatat
     8521 ccgtctgtcc ccgatcagcc gctttttgcc gctgctgcgc cgacgcaatc gccgtttcca
     8581 gcgccttcag caccgcattg cgctcgccga acccctccag tgcgccgacc ttgcagagat
     8641 tctcagcgac gcgccgcccg actttggacc agctcacgga atcacagaag tcctccagcg
     8701 acgcgaactc gccggtcggc tgctccttcc gtgcagcgac gatcgtctcg acagcaccga
     8761 cgccgacgtt tctgatggcc cccatcgcga atcggatcgc ctccgtccca tcgtccagct
     8821 tctcgaccga aaactcgaca ccgctccggt tgacatccgg cggcaggata gggataccgg
     8881 tacggcgaca ctcggcgatg ttgaccaccg ttttctcgct gctgttcatt tccgtcgaca
     8941 tgagcgcggc catgaactgc accgggaagt tcgccttgaa gtagcccgtc tgcgcggcga
     9001 tcacggcgta ggcggcacta tgagccttgt tgaaaccgta cccggcgaag gcctcgatca
     9061 tgtcgaagat ctgcttcgcg aggtccttgt cggtcccgtt ctcgaccgct ccctcaataa
     9121 agcgcgactc gtacttggcc atcacctccg gcagcttctt gcccattgcc ttgcgaagcc
     9181 cgtcggcctc cgccatagag aagcccgcca cggcgttggc aatctgcatc acctgctcct
     9241 gatagatggc gatgccgtac gtcttctcca ggatgggctc aacgagcggg tggatatact
     9301 cgaccggctc ccggccgtgc ttgcgggcaa tgaagtcggg cgcgagctcc agcggccccg
     9361 gcctgatgag ggccatcagc gcaacgaggt cgtcgaaact ctccggcgcg acgtcgatgg
     9421 tcatccgccg ggtcatgccg ccttctagct ggaacacccc gaaggtctca cctcgacgga
     9481 gcagcgccag ggcttccggg tcctccaagg gagtgtcttc cagggatatg ttgtgaccgg
     9541 actcggcgag cagcgcgatc gctttcccca gcaccgtcaa cgtcttgaga ccgagaaagt
     9601 ccatcttgag caagccgagc gactcaaggt ggtcctgcgt ccactgcgtt gtcacatctc
     9661 cctcgctctt gccgcccgcg tactgcagcg gcacctgctc gaccagcgga tcgcgggaaa
     9721 tgacgacacc ggcggcgtgt gtggacgagt ggcgcgcgtg gccctcgacc ttcttcgccg
     9781 tgtcgatgag ctcacgcacc tgcgcatcgg actcgtagag cttgccgagg tccggcacgg
     9841 tctcgagcgc cttctcgatc gtcattttcg gatcggtcgg gatgagacgt gcgacccggt
     9901 cggtctcacc gaagcccaaa tccatcgccc gccccacatc gcggaccgcc gccttcgcct
     9961 tcagcgtgcc gaacgtgatg atttgcgcga cccgatcgtc gccgtacttg tcgacgacat
    10021 actcgatcac ttcgtcacgc cggtcgtcag cgaagtcgat gtcgatatcc ggcatggaga
    10081 tacgggacgg gttgaggaag cgctcgaaga agagctcgta cttcagggga tcgagaccgg
    10141 tgatgtccaa cgcataagtc acgacgctgc ccgcggccga gcctcgaccg ggcccgacca
    10201 ggatgccgcg ttccttcgcg aaccgcacga agtcccagac aatgaggaaa tagttggtga
    10261 agcccatgtt ctcgataatg ccgagctcgt ggtcgatgcg ctcacagaca gcaccttcaa
    10321 tggttccata cttccggcga gccccctcct cgaccagctt cctcaggtac gccgacggcg
    10381 tgaattcctc aggcacgtcg aagtgcggca gttggtagcc cttgaagccg agatcaagcg
    10441 agcacatctc cgcgatgcgg accgtgttgg tgattgcttc tgggatatgt ccaaagagcc
    10501 gctccatctc ccccgccgac ttcagataca gctgcgtgga gtcgctcttg agtcgcttcg
    10561 gatcgttcag cgtggtgttg gtctgtacgc agacgagcag atcctgggcg gcagcgtcgt
    10621 cctcttcgtt gtagtgcacg tcgttggtgg caacgatcgg caggttcatc cgcttcgcga
    10681 gcggaatcat gaacctattc gcctcttcct cgagatcgat tccgtggttc tggatttcga
    10741 tgaagaagcg atcccttccg aagatctctg atagcgtccc ggcatagcgc tcggcccgct
    10801 cgatgtcgcc attgaggaac ggcttggcca ccggaccgcc gatgcaggcc gaggtggcga
    10861 tgatgccctc gtggtactgc tgcaacaact cgaggtcgat tcgcgggcgg tagtagaagc
    10921 cctcgaggct ggcgatgctg gcgagcttca gcaggttacg gtagccggtc tcgttcgccg
    10981 ccagcagcag gagatggtac gactcccgct tgcgctcgct gatcaggccc tccgccaggt
    11041 atgcctccat gccgatgatc gggtggagcc ctgcttcagt ggctgcgttg taccaggaga
    11101 gtgaggcgta catgacaccg tggtcagtga ccgcgatgtg ctgcaagccg agctgatgtg
    11161 cccgctcgat gtactggtcg atgcgaccga cgccatcaag caacgaaaac tcggtatgca
    11221 ggtgcagatg cgcaaattct gccacggtga aaacctctcg gctgctccag atgtcgaact
    11281 cggactcccg cccagaaccg ttgaagagag ttagcccctg agcgatacgg ggggtaagcc
    11341 cagtgtctca tattctggaa ggcctgtacg tgcgggttcg aaggggcggc gcatccccgc
    11401 tcgccgcccc cttgaacccg cgacggatca ggccgcgaag cccgctccgg ctgctttctc
    11461 ccccttgagg acaagccggg cctgatcagc caggacacgc agtacctgcc gcatcgagga
    11521 tcggtactcc tcaggcacct ccgcgttctg caggagattg agcacctcgg tgatctcggg
    11581 ctcgccggca aggaggctcg acacctcgcg tggcagaaag ccggcggaga ccaacagttc
    11641 atcctgctgc acgccgtcga gctcgagtgc cccggcgagc tgctcgacag cttcgcgggt
    11701 cggcgttcgg gcgccgctct cgaggcggct cacgtaggag tgatcgaacc ccgcccgctt
    11761 ggcgagcttg ctctgcgata cgcgccgctg ctctcgaaac tccttcagca cgcggccgaa
    11821 cgccgagcct gcttcccgca cctgaacagc catgattatg ctccccactt tccggcggcc
    11881 cacctcggta tgccaccggc ccgagttgcc aaacagtcac gacacgactg ccaactgatc
    11941 ggattgtact accgggttgc cggcctgtca acaacccgac gggcggcggc ttgccgcggt
    12001 gtcacccaac gagttgtaag cgcgcgcagg agcgggtgca tcacccctgg cgttgatgga
    12061 aacgacagcc gctatcgctt gctgtcttaa tgtggcgtga tcatggtctt ttcggggatt
    12121 tgtgctgtgg aaaggggagg ccggtctccc acggagcgcc tgatatactg ccgattgacg
    12181 aaactgcccc tggtggcgcc actggggccg cctatctcac acgtcggagt cgcctcgtgc
    12241 cagccgatgg accggagccg gtcgaaacct acgtcctgaa gggctctcgc ctcgcgaagc
    12301 ggctgcgtgc ggagatcgct gcctcccagc agcacctgct cgacgttgga ggcgaaccgc
    12361 cgcgcctcgc aacattgatc gtgggcgagg atcgggcggc catcgcctac cggtcgtcga
    12421 tcgaacgcac cttcgcccgc gccgagatcg acgtcctgcg agcggaacta ccgggcgatg
    12481 tgtccgagca gaccttcctt gaggagttgc gggagctgaa ccgggatccg caggtgacgg
    12541 cggtcctggt cctcatgccg ctgccggccc acttaaaccc gcatctcgtg ctggaaaacc
    12601 tctcgccact caaggatgtg gacggcatca cacccaccaa cgccggtcgg ctccacctcg
    12661 gcttgccctc gctccgcccc agcacgccgg cgggcggact tgccctgctc gaccactacg
    12721 gcgtaacgct cgaaggagcg cgggttaccg tcgttggccg ctccaacgtc gtcggtcggc
    12781 cgctggcgac cctgctcacc cagcgtgacg caacggtcac gctctgccac tcaaaaaccg
    12841 tcgacctcga cagggaaacg cgccgcgccg aaattgtctg tgtggcggca ggtcatgctg
    12901 ggttgatcag ccgggatatg gtgtctcggg gtacagtggt gattgacttc ggcgtaaacg
    12961 ttaccgacga gggcaagctg acaggtgatg cagatttcga cggactcatg gggatcgccg
    13021 gggctatcac tccggtcccc ggcggcacag gcccggtcac cgcgcttacg ctcgcccaga
    13081 acaccatggc cgccggcttc gcggcacttg gcggcaacct cgactacgtc gactacgcgc
    13141 tgccagaccg cctccagcaa aagatcaatg acgctaccag ggagggagtt gaacagccca
    13201 ccgcctcgtg atacacttac gtgcgctcga tggggtgtcg tctaacggta ggacgcctga
    13261 ctctggatca ggtagttggg gttcgaatcc ctgcacccca gccatcgaaa cagccagaag
    13321 ccataggggg taaacgcaga accccggcca catcggccgg gggtttctcg tgggcgtgac
    13381 agccaagatg acagccaaac ggacgagagt tggcagtatc aggcgagagt cgagccggcg
    13441 ttttttcccg tttttcgagc gcaacgagac tcggtgagat gggccgagac atccggaatc
    13501 ggactctgga caagcttgtc ggggtttgaa tcccgcgccc caggcccaaa ggcaggctcg
    13561 acgtgcgagg agaatggcag acccgggctg aatctgccag ggttcttcgc tgccattgac
    13621 agccattagg tcgggtcccc tctcccacta ggcaggaccg ccgggcacgc gaacgccctc
    13681 gcttgcggga gcgtgcccgg cggtgactca cctacgcggc agcatcagct gctcgctggg
    13741 gcgccgcgtc gttgtccccc cgcccgacgc catgcagcgc gtcgtggtgg cctgtccgtc
    13801 gaagattgga acgagcctgg tcgggcgggc gcccgtgcct ctgggccgac tatcgcacgt
    13861 gcgcgtcgcc ggcctcctcg gcgggccgtc cctccaggat catcaccttg acatgcgacg
    13921 ccagcgcgcc cccggcaagg atgatgagcg caatgtacca cccccaggac agcaggagcg
    13981 ccgcgaccgc gagtggcccg tagatcagtg tgaggttggg ccagaggaca tcgaagagga
    14041 agagcacgac aaacactccc ctcgccccca gaaagagcgc ggtggcgacc accgcgcccg
    14101 cgaacgccgc ccgccagaac cgctcacccc ggggcacgac cacgtagacg agggtgaaga
    14161 ccaccaccag caggccggct cggaccagct gatgcgcgac cggcagcagc gcgccgtcgc
    14221 gtccgggcag gatcccgatc atgccccaca gcagttccag cagcgggcgc gacgcaaacg
    14281 cgaggagcag cgccccgatg agcccggtga tgaggctgac ctctacggcg gcccgacgtt
    14341 ccagcgggtc gtcccgctcg tcaacatcgg acaccaggtt gagcgccttg gtcaccgcgc
    14401 caagcacgcg tcgcccggtg accagcagca ccgcgagcgc gaggacgccc gtacggccgc
    14461 gctgagcgat ggcgtcctcg acgatcgcct cgatcgtctc ctggccctcg ggaagcacag
    14521 caccgagcag gacggtcgcc ttggcggtcg cccatgccgg atcgacgaac cacgaggcgg
    14581 caatcatccc cgcg
//
LOCUS       DASYWL010000057         4197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_115210, whole genome shotgun sequence.
ACCESSION   DASYWL010000057 DASYWL010000000
VERSION     DASYWL010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115210"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VGR22_01980"
     CDS             complement(<1..795)
                     /locus_tag="VGR22_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127367.1"
                     /translation="MRERSTTLGLAPALPGIVLVVVIVWALAAVLMLTGTLINAREID
                     RAVPIINSSVLPIDEDLDNVKLAEETARLTEKIRDAAAPLSDQADRIIAEAGKIDRNA
                     ASILVTARSINDTAKAINSNALSINDTVSTINSNVVSINGLVDSIGASVFSIGSRVST
                     VGTNVASINRRVGTVLGAVGASNATGDGSIRASVDAILATFVALEPVTRSIESEDELG
                     GVAGINRRADDGIEGARLIKGDFEGILELVGRGALQAPGPHETAGPG"
     gene            complement(811..1698)
                     /locus_tag="VGR22_01985"
     CDS             complement(811..1698)
                     /locus_tag="VGR22_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127368.1"
                     /translation="MREAAPTFIRWLWIWVTIAALVVVVVIGFLIGIVSSLSSIDDNL
                     FEADAAVTGISGDAEPLPDAVETINKNLRLIDASLEPIPDQADTILNSLGSIRGKLGA
                     IDSSLGDTDASLENTDASLVDTSNQLGNITSLLNDTAGTLVTISGSLDDTSGTLQGIS
                     PSLDDTSGMLEVISPSLDDTSGELVSVLALARRIDTTLKAAQRSEAGEGDDFHLGTNG
                     IWRRVRFLNGGAFDRPGDDKDTSLAGPNDNPNGLEPVEDDATAIIGGLIEINKHLTSI
                     CEAPVLQVPIPLLVNPGPC"
     gene            complement(1711..2808)
                     /locus_tag="VGR22_01990"
     CDS             complement(1711..2808)
                     /locus_tag="VGR22_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127369.1"
                     /translation="MLVIVVAVIGIAAVLLARTVQEAQSINAKAQNIATNGRGINEAT
                     DSVIQLNRTVDLAKSILESARPLDTQLGRIVATASGINSRAASINSKAGSINQTAGSI
                     NDTAGAINTSAGEINAAAESINSSAGSVNTTAGAINQSAGSINTSAGAINQSAGSINT
                     SAGTINATARGIQDEASSILKTARLIDTDVELINENLDVSLNLVTAVKADTENILGQA
                     AEANQTAACIDRKLNGQAGNDGDCQGQPQAQPEPAQPVSNQRMSRKAYEGLLKKTAPP
                     APKAEKPKETKTVKRRKVTIPLSERLPETPTTEQVNKIVEDLLGRTKLGRSTDGLLRS
                     KDGLLRGTKDADKRRDALGRLLPELPLLGQR"
     gene            complement(3009..3674)
                     /locus_tag="VGR22_01995"
     CDS             complement(3009..3674)
                     /locus_tag="VGR22_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127370.1"
                     /translation="MARQSVREHELRAAVDRLPLATRRAMLEGLETQRIIVGADGSPG
                     GGVCPIYATANPPSKTIGAPFARAWDRFAGARLARPASEREVRTLRRMLEASIGLATE
                     PAEPAVSLSAAIAAHKATQARNRAIQSVPEMPAVPERPSVSERRSPARPMRKPAPRRD
                     TGERERTAELSPQHGWAWLRPFRSYDDYERAMLALEGMVPQAEARPEADSERLVTSGS
                     GRR"
     gene            3916..>4197
                     /locus_tag="VGR22_02000"
     CDS             3916..>4197
                     /locus_tag="VGR22_02000"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HEV2127371.1"
                     /translation="MLGVAYVAAGLIGFAVTGFTGPVVLNTDDQLLGFFDLNIFHNIV
                     HIAIGGGLIVAGLMRDAAITQGVLIGVGLFYVVAALLGFLNYLQIISIDS"
BASE COUNT          579 a         1375 c         1447 g          796 t
ORIGIN      
        1 ccccggcccg gcggtctcgt gcggcccggg ggcttggagc gcgccccgtc cgacgagctc
       61 gaggatcccc tcgaagtcgc ccttgatcag gcgagcaccc tcgatgccat catcggcgcg
      121 ccggttgatc ccggcgaccc cccctagttc gtcctcgctc tcgatcgacc gcgtcaccgg
      181 ctcgagcgcc acgaacgtgg ccaggatggc gtcgaccgac gcccggatcg aaccgtcccc
      241 ggtggcgttc gacgccccga ccgcgccgag caccgttccc acgcggcgat tgatcgacgc
      301 gacgttggta ccgacggtgc tgaccctgct gccaatcgag aagacgctcg ccccgatcga
      361 gtcgacgagg ccgttgatcg acaccacgtt gctgttgatc gtgctgaccg tgtcgttgat
      421 cgacagggca ttgctgttga tcgccttggc cgtgtcgttg atggacctgg cggtcaccag
      481 gatcgaggcg gcgttgcgat cgatcttgcc cgcttcggcg atgatgcggt cggcctgatc
      541 ggagaggggg gcggccgcat cacggatctt ctccgtgagg cgggccgtct cctcggcgag
      601 cttgacgttg tcgaggtcct cgtcgatcgg cagcacactg ctgttgatga tcgggacggc
      661 cctgtcgatc tcgcgtgcgt tgatcagcgt gccggtgagc atcagcaccg ccgcgagcgc
      721 ccagacgatc accacgacga gcacgatccc cggcagtgcg ggcgccagcc cgagtgttgt
      781 gcttctctct ctcatggtcg tccgtcctac ctagcagggg ccgggattca cgaggagggg
      841 gatcggcact tggagcaccg gcgcctcgca gatgctggtg aggtgcttgt tgatctcgat
      901 cagcccaccg atgatggcgg tcgcgtcgtc ctcgaccggt tccaggccat tggggttgtc
      961 gttggggccg gccagcgagg tgtccttgtc atcgccgggc cgatcgaagg cgccgccgtt
     1021 gaggaagcgc acccgtcgcc agatgccgtt cgtgccgagg tggaagtcgt ccccctcccc
     1081 tgcctccgac ctctgggccg ccttcagcgt ggtgtcgatc cttcgggcca acgcgagcac
     1141 actgaccagc tcgccggagg tgtcatccag cgagggtgag atgacctcga gcatgccgga
     1201 ggtgtcatcc agcgagggtg agatgccctg gagtgtgccc gaggtgtcgt ccagcgagcc
     1261 cgagatggtc accagcgtcc ccgcggtgtc gttgagcagg ctggtgatgt tcccgagctg
     1321 gttggaggtg tccaccagcg aggcgtcggt gttctccagc gacgcgtcgg tgtcgcccag
     1381 cgaggagtcg atggctccca gcttgccgcg gatcgacccc agcgagttga ggatggtgtc
     1441 cgcctggtcc gggatcggct ccagcgacgc gtcgatcagc ctcaggttct tgttgatggt
     1501 ctcgacggcg tcgggcagcg gctcggcgtc accggagatg ccggtgaccg ccgcgtcggc
     1561 ctcgaacagg ttgtcgtcga tcgacgacag cgagctgacg atcccgatga ggaacccgat
     1621 gacgaccacc acgacgagcg cggcgatggt gacccagatc cagagccacc ggatgaacgt
     1681 gggtgctgct tcacgcatgt ctatcgcccc ttatcgttgg cccagtaggg gcagttcagg
     1741 gaggagtctt cccagtgcat cacgccgctt gtcggcgtcc ttggtcccgc gcagaaggcc
     1801 gtccttgctc cggagaaggc cgtccgtgga ccggccgagc ttggtccggc ccagaaggtc
     1861 ttcgacgatc ttgttcacct gctcggtcgt cggcgtctcg ggcagccgct cgctcagcgg
     1921 gatcgtcacc ttccgccgct tgacggtctt cgtctccttg ggcttctcag ccttgggcgc
     1981 cgggggtgcg gtcttcttga ggagcccctc gtaggccttt cgcgacatcc tctggttcga
     2041 gaccggctgg gccggctcgg gctgcgcctg gggttgtccc tggcagtcgc cgtcgttgcc
     2101 ggcctggccg ttgagcttcc gatcgatgca ggcggcggtc tggttggcct ccgctgcctg
     2161 gccgaggatg ttctccgtat cggccttcac cgccgtcacc agattgaggg acacgtcgag
     2221 gttctcgttg atcagctcca cgtcggtgtc gatcaggcgg gcggtcttga ggatggagct
     2281 cgcctcgtcc tggatgccgc gggcggtcgc gttgatcgtg ccggcgctgg tgttgatgga
     2341 gcccgcgctc tggttgatcg cgccggcgct ggtgttgatg gagcccgcgc tctggttgat
     2401 cgcgccggcc gtggtgttga cggagcccgc gctggagttg atcgattcgg ccgcggcgtt
     2461 gatctctccg gcgctggtgt tgatggcccc cgcggtgtcg ttgatgctgc cggccgtctg
     2521 gttgatgctg ccggccttcg agttgatcga cgcggcgcgc gagttgatgc ccgacgccgt
     2581 ggcgacgatg cgccccagct gggtgtcgag cggccgggcc gactcgagga tcgacttagc
     2641 cagatcgacg gtgcggttga gctggatgac cgaatcggtc gcctcgttga tcccccgccc
     2701 gttggtagcg atgttctgcg ccttggcgtt gatcgactgc gcttcctgaa ccgtgcgcgc
     2761 caacagcacg gcagcgatcc cgatcaccgc gaccacgatt acgagcacga ttacgctcgt
     2821 gaaggtaaaa cctctcatac gtccagcacg cctccctcac cgattctctc ccggacctgc
     2881 gacggcgacg agactaaacg tatatgcggc cgacatacaa ccccgaaccg tcgaaggacc
     2941 cgtttgcggg tccttcgagg tcctggcgga gggacgctgg gcgcgttccc tgaagggtcg
     3001 gccgaaagtc agcggcgccc ggagccgctc gtgacgagcc gttcggaatc ggcctcgggg
     3061 cgtgcctctg cctgggggac catgccctcg agtgccagca tcgcgcgctc gtagtcgtcg
     3121 tagctacgga acggacgcag ccacgcccag ccgtgctggg gcgacagctc cgcggtgcgc
     3181 tcccgctcgc cggtgtcgcg tcggggtgcc ggcttgcgca tcggcctggc gggcgagcgc
     3241 ctctcggaaa cgctcggcct ctcgggaacg gccggcatct cgggtacgga ctggatggcc
     3301 cggttgcgcg cctgagtggc cttgtgcgcc gcgatcgccg cgctcaggga gaccgccggc
     3361 tcggcgggct cggtggccag cccgatgctc gcctcgagca tcctgcgcag cgtgcgcacc
     3421 tcccgctcgc tcgctggacg ggccagccga gcgcccgcaa accggtccca ggcccgcgcg
     3481 aagggcgcgc cgatcgtctt ggatggcggg ttcgctgtgg cgtagatcgg acagacacca
     3541 ccacccgggc tgccgtccgc cccgacgatg atgcgctggg tctcgaggcc ctcgagcatc
     3601 gcccgccgcg tggccagcgg cagtcggtcg accgctgctc gcaactcgtg ttcccttacc
     3661 gactgtcgtg ccattggcgc ctccgcttca gcgttgatct ccggactgcg agtggtccga
     3721 ggctagggcg ggccatcgcg gcaatcaagc gtttgcctgc cggtgcacgg gaagtttcag
     3781 gcgacgatct gcgcgggcgg gcgcgaggac gtctactgta tgcgcgcgac acgcagtatt
     3841 cgtcgagggt cgcctaccgg gagagggaga gagaaaatgg gagcaagtag cacagtggca
     3901 cgaggagccg cgctcgtgct cggggtcgcg tacgtggccg caggactgat cggctttgcg
     3961 gtcaccggct tcacgggccc cgtggtcctg aacaccgacg atcagctctt gggcttcttc
     4021 gacctgaaca tcttccacaa catcgtgcac atcgcgatcg gcgggggcct gatcgtcgcg
     4081 gggctgatga gagacgcggc catcacccag ggggtgctca tcggcgtcgg gctcttctac
     4141 gtggtcgccg ccctgctcgg gttcctcaac tacctgcaga tcatctcgat cgacagc
//
LOCUS       DASYWL010000058         2790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_209043, whole genome shotgun sequence.
ACCESSION   DASYWL010000058 DASYWL010000000
VERSION     DASYWL010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209043"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1867)
                     /gene="gyrA"
                     /locus_tag="VGR22_02005"
     CDS             complement(<1..1867)
                     /gene="gyrA"
                     /locus_tag="VGR22_02005"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEV2127372.1"
                     /translation="MDIGNVRTASIESEMRQSYLDYAMSVIVQRALPDARDGLKPVHR
                     RVLYAMHQMGLRPNTRYRKSAGIVGEVIKNFHPHSDTAAYDTLVRMAQDFSLRYPLVD
                     GQGNFGSVDGDSAAAMRYTEARMTAIAEEMLADINKRTVDEYPNYDATMTQPSVLPSR
                     IPNMLINGSAGIAVGMATNIPPHNLNEIADAITHLIDHPDATVEELIEIVPGPDFPTG
                     GTILGHEGIHAAFATGRGRVVIRAKAFIEDMDRGNRSQIVVTELPYQVNKATLLERIA
                     EMVRDGKLDGISNLRDESDRSGMRMVIELKRDAQPKKVLNNLFKHTALQQTFGVNMLA
                     LVDRGMQPRVMTLKRALQEFINHRQEVITRRTEFELERARNRAHILEGLKIALDNIEA
                     VISTIRRSRTTDTARRNLMKNFSLSQAQADAILDMRLARLAALERQKIEQEYREVMAE
                     IGRLEELLASPELILGIIKADMEDLKEKYGDERRTRIQNVSGELSDEDLIPETDVLVT
                     ITNRGYVKRINGDSYRTQRRGGRGVTGLTMRDEDGIQHILSANTHDSLLVFTNRGRVY
                     QIKVHELPDAGRTAKGLPIVNVINLQADENVATLLKVRHHDPGNYLFFASRKGRVK"
     gene            complement(2194..>2790)
                     /locus_tag="VGR22_02010"
     CDS             complement(2194..>2790)
                     /locus_tag="VGR22_02010"
                     /inference="COORDINATES: protein motif:HMM:NF024813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEV2127373.1"
                     /translation="DFLRSMILNEFNDMLGDVHTSILEIQSMTREIADATRSALASDF
                     TRLGLDLVSFQISAITPPEEVQQRIDERSGMGAIGNMGAYTQYKTAQAIGDAARAEGG
                     GGDAASTGVGLGAGLGIGQAMAGAIGSAYQQSQQGQQQQGQQMATRQCPNCNSTIPAN
                     AKFCPECGTSLAETVCPECGTANQPGAKFCTNCGTRLE"
BASE COUNT          413 a          879 c          931 g          567 t
ORIGIN      
        1 gcttcacacg gcccttgcgg gacgcgaaga agaggtagtt gcccggatca tggtggcgca
       61 ccttcagcag cgtcgccacg ttctcgtcag cctgcaggtt gatcacattg acgatcggca
      121 gccccttcgc cgtgcggccg gcgtccggca gctcgtgaac cttgatctgg tagacgcgac
      181 cccggttggt gaagaccagc agcgagtcgt gcgtgttggc gctgaggatg tgctggatgc
      241 cgtcctcgtc gcgcatcgtc aggccggtga ccccacggcc gccacgccgc tgggtccggt
      301 agctgtcgcc gttgatgcgt ttgacgtagc cccggttggt gatggtcacc agcacgtcgg
      361 tttccgggat gagatcctca tcgctcagct cgccgctcac gttctggatg cgggtgcgcc
      421 gctcgtcgcc gtacttctcc ttaaggtcct ccatgtccgc cttgatgatg ccaaggatca
      481 actcagggct cgccagcagc tcctcgagcc gcccgatctc cgccatgacc tccctgtact
      541 cctgctcgat cttctggcgc tcgagggcgg ccaggcgagc gaggcgcatg tcgaggatgg
      601 cgtcggcctg cgcctgggag aggctgaagt tcttcatcag gttcctgcgc gccgtgtcgg
      661 tggtgcgcga gcgccggatg gtgctgatta ccgcctcgat gttgtcgagg gcgatcttga
      721 ggccctcgag gatatgggcg cggtttcggg cacgctcgag ctcgaactcc gtccggcggg
      781 tgatgacctc ctgccggtgg ttgatgaact cctgcagcgc gcgcttgagc gtcatgacgc
      841 gcggctgcat gccgcggtcg acgagcgcca gcatgttgac gccgaacgtc tgctgcaggg
      901 ccgtgtgctt gaagaggttg ttgagcacct tcttcggctg ggcgtcgcgc ttgagctcga
      961 tgaccatccg catgccggag cggtccgatt cgtcgcggag gttggagata ccgtcgagct
     1021 tgccgtcgcg gaccatctcg gcgatgcgct ccagcagcgt cgccttgttg acctgatacg
     1081 gcagctcggt gacgacgatc tggctgcggt tgcctcggtc catgtcctcg atgaacgcct
     1141 tggcccgaat caccacgcgg ccacggccgg tggcgaatgc ggcgtggatg ccctcgtggc
     1201 cgaggatcgt gccgccggtc gggaagtccg gtccgggaac gatctcgatc agctcctcga
     1261 cagtcgcgtc tgggtggtcg atgagatgcg tgatggcgtc ggcgatctcg ttcaggttgt
     1321 gcggcgggat gttggtggcc atgccgacgg cgatgccggc gctgccgttg atgagcatgt
     1381 tcgggatgcg cgacggcagc accgacggct gcgtcatcgt cgcgtcgtag ttggggtact
     1441 cgtcgaccgt gcgcttgttg atgtcggcga gcatctcctc ggcgatcgcg gtcatgcgcg
     1501 cctcggtgta gcgcatcgcc gcggcactgt cgccgtcgac cgagccaaag ttgccctgac
     1561 cgtcgaccag cgggtagcga agcgagaagt cctgcgccat gcgaaccaat gtgtcgtagg
     1621 cggcggtgtc gctgtgcggg tggaagttct tgatgacctc gcccacgata ccggcactct
     1681 tgcggtagcg tgtgttgggc cgcagcccca tctggtgcat ggcgtacaac acgcggcggt
     1741 ggaccggctt gaggccgtca cgagcgtccg gcagggcccg ctgcacgatc acgctcatcg
     1801 cgtaatcgag ataggactgc cgcatctcgg attcgatgct cgcggtacgc acgtttccaa
     1861 tgtccacgcg ttgagctcct gctagattcg agtcattacg ggggcgcccg gcatgggcgg
     1921 aacaccttat tttaccagaa tcccggccag aatggccggg atgtcgtaca taagactacg
     1981 ttgtaaccac cgtgcgagct ctaccgaatc tgggaattca atcggggaat ccgtcagtcc
     2041 gcaggggtcg atgctgtcac gacacggccg cctgcagggc gacagctgcg ggctgggagc
     2101 atctcacaga tccgcacgct ccgcgcatgc tggacggaca cagggtgcgc cactgcgagc
     2161 cgatgtttac caacggttca gccggattcc tcgctactcc agtctcgtcc cgcaattggt
     2221 gcagaacttc gctcccggct ggttggcggt accgcactcc gggcacaccg tttcggccag
     2281 actcgtgccg cactccgggc agaacttcgc gttcgccggg atggtggagt tgcagttcgg
     2341 gcactgccgg gtcgccatct gctgaccctg ctgttgctgc ccttgctggc tctgctggta
     2401 ggcgctgccg atcgcgccgg ccatcgcctg gccgataccg agccccgccc cgagcccgac
     2461 gccagtggac gcggcgtccc cgccgccacc ttcggccctt gccgcatcac cgatcgcctg
     2521 ggcggtcttg tactgggtgt atgcgcccat gttgccgatc gctcccatgc cggagcgctc
     2581 gtcgatgcgc tgctggacct cctccggcgg cgtgatcgcg ctgatctgga acgacactag
     2641 atcgaggccc agccgggtaa agtcggaagc cagtgccgag cgggtcgcgt cggcgatctc
     2701 gcgcgtcatc gactggattt ccaggatcga tgtgtggaca tcccccagca tgtcgttgaa
     2761 ctcgttgagg atcatgctgc gcaggaagtc
//
LOCUS       DASYWL010000059         6663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_359044, whole genome shotgun sequence.
ACCESSION   DASYWL010000059 DASYWL010000000
VERSION     DASYWL010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6663
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359044"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1752
                     /gene="uvrB"
                     /locus_tag="VGR22_02015"
     CDS             <1..1752
                     /gene="uvrB"
                     /locus_tag="VGR22_02015"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEV2127374.1"
                     /translation="FVSYYDYYQPEAYVPRTDTFIEKDADINEEIDKLRHAATRSLLT
                     RRDALIVASVSCIFGLGTPEEYANTIVSLRRGDSVRRDKILRQLVDIQYDRNDMNLVR
                     GTFRVRGDTLEVFPAYEEIAVRVEFFGDEIERIVEVDPLTGELLIQRTEIDIYPAKHF
                     VTTQDRMGRAIEDIQDELAERVKFLEENGQVLEAARLKQRTMYDIEMMQEASYCAGIE
                     NYSMHLSGRKPGDQPSTLLDYFPDDFLLFVDESHITLPQVRGMFGGDRSRKDVLVEHG
                     FRLPSARENRPLTFEEFERTIGQAIFVSATPGPYEYEHSSRVVEQVIRPTGLVDPAIT
                     VKPTKGQVDDLLGEINQRVAKGERTLVTTLTKRMAEDLSDYLKEMGIRTHYLHSEIDT
                     LERIEILRDLRLGIHDVVVGINLLREGLDLPEVSLVAILDADKEGYLRSEGSLIQTIG
                     RAARHVDGHVIMYADTMTRSMRAAIDETYRRREKQIAYNEEHGIEPRGIVKAIKDLTD
                     RVREVAEQTASYDTGGGPAEGVIHQLPKDELTRMIKDLESQMKKAARDLEFEKAALLR
                     DQVVELRRVMEADPIEA"
     gene            1779..2738
                     /locus_tag="VGR22_02020"
     CDS             1779..2738
                     /locus_tag="VGR22_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127375.1"
                     /translation="MYHRGFARTALVALLTVLLLAMPSLSVAQGGTPSPGDESELAPQ
                     LPTVDLPTLNEMNYRFELESVWTGSAGTPAELPVYLFEPIVYTEEDVARIAEELGVEG
                     EITSQGAGSYSVSGNGSIFTSPGLLQYVSAAEPPDEDLPSNAEAVALAHEWLRTTGLL
                     PANIGEGEVLATIETPARKIVGFQPATPSPLLSSTPGITVTIGPDATVIEARINWAEI
                     TESDVYLLRPVEDAFAQVASRQSYINATLPADQFPQGSTISGTATYDSVSIAYASSGV
                     PGETQFLQPVYVFEGSVDVAEAEGTFDIVAYVPAIVTGLEPVG"
     gene            2764..3213
                     /gene="aroQ"
                     /locus_tag="VGR22_02025"
     CDS             2764..3213
                     /gene="aroQ"
                     /locus_tag="VGR22_02025"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEV2127376.1"
                     /translation="MKLLLVNGPNLNLLGTREPEVYGHQTLADIVDAVRREAAAHDCD
                     LLDFQSNHEGAILDFIHENAASADGLIINPGALTHYSIALRDAISGTGIATIEVHLSN
                     VHTREAFRHHSAISPVAAGTIVGLGAHGYHLALQWFRRTIQPVQKGS"
     gene            3213..4322
                     /locus_tag="VGR22_02030"
     CDS             3213..4322
                     /locus_tag="VGR22_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEV2127377.1"
                     /translation="MSVIARLKNARQVIRDTGLDAIVVTNPTNRRYLTGFTAEDHAAD
                     ESAGVVLLTAENATLIVSPTNLPWAAAEADAESITVKPHNGDMPGTIAERLTGIDATT
                     VAVEDLTTPTAVWFALRASLGERVEMVRASDCIDQFRAIKTADELEHLREAARLTDTA
                     FAIAVDRLRKGVTEREAADVVRDALREVGSEGEAFETIVSSGPNAAKPHHRPGARRLA
                     AGEPIVIDMGARVSGYNGDLTRTVSLGKADEKLGRIYGVVLEAQLAGLDAIRAGVSAA
                     RVDQATREAFAAHGLEQHVVHSAGHGLGLRVHEAPSVRKTSEETLDAGHVITMEPGLY
                     IEGWGGVRIEDVVIVRNTGHENITTAPKGLDSLEH"
     gene            4344..4904
                     /gene="efp"
                     /locus_tag="VGR22_02035"
     CDS             4344..4904
                     /gene="efp"
                     /locus_tag="VGR22_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEV2127378.1"
                     /translation="MIDTGDIRKGSTFEQDGRLLKVVDFSHNKQGRGSAQLRMTLRDL
                     RTGSLTQQTVMAGTKFNSVRLERSHVQFLYREGDAFHFMDTETFDQILVMESTIGDAA
                     QYIKENDVIDVLTYEDEPIDIELPTSVNLAVTQTDPGFKGDTATGGSKPATLETGLTV
                     NVPLFVSEGDQIKVDTRTGEYIERVG"
     gene            4935..5405
                     /gene="accB"
                     /locus_tag="VGR22_02040"
     CDS             4935..5405
                     /gene="accB"
                     /locus_tag="VGR22_02040"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEV2127379.1"
                     /translation="MNRTDQEASSTQELIGAVRELISMMGKGGISELDLTSGDVSIRL
                     RGGTAAVASPAPSVMPNGEMDDPEAAEDEGHLITAPMIGTFYAAPSPGEEPFVQVGDE
                     VEVGQVVGIIEAMKIMNEIVADRPGMVAEILVENAQAVEYGSPLVRLAEQTDEL"
     gene            5405..6637
                     /gene="xseA"
                     /locus_tag="VGR22_02045"
     CDS             5405..6637
                     /gene="xseA"
                     /locus_tag="VGR22_02045"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEV2127380.1"
                     /translation="MTSHILPVSTLNAYIQQVVSADDILADVWIEGEISQLTKARSGH
                     VYFKLCESDALIDCAMWKHSAARQTFIPRVGDEVIIHGHADFYPPQGRLQIIADVFEH
                     QGQGILALEMERLRQKLEAEGLFDLARKRELPPFPRRIGVVTSSAGAVWHDIQTVTRR
                     RFPLVELVLISASVQGSNAPREIVAAIRQMCELGNIDVMIVGRGGGSPEDLACFNSEE
                     VARAIFASTVPVVSAVGHETDVSIADMVADVRAATPSAAAEIVLPDIRELAVLLADTR
                     EELIEIAERRLANERSRLGALTRRFHQQSPRAHISRTEQELATLERRLRLSGRSYLQQ
                     RETSLRSTAKVLAALDPTRVLERGYAVIATPAPPSPIRRMADLDGDTAVVVNFRDGTA
                     RGTIAPHTNDSQEDAQQS"
BASE COUNT         1301 a         2204 c         2062 g         1096 t
ORIGIN      
        1 ttcgtctcct actacgacta ctaccagccg gaggcgtacg tcccgcgcac tgacactttc
       61 atcgagaaag acgccgacat caacgaggag atcgacaagc tgcgccacgc cgcgactcgc
      121 tcgctcctca cgcgacgcga cgcgctgatc gtcgcctcgg tttcgtgcat cttcggcctc
      181 ggcacgccgg aggagtacgc caacacgatc gtctcgctga gacggggaga ttcggttcgc
      241 cgcgacaaga ttctgcggca gttggtcgat atccagtacg accgcaatga catgaacctc
      301 gtgcgcggta ccttccgggt tcgcggcgat accctcgagg tcttccccgc ttatgaggag
      361 atcgcggtgc gtgtcgagtt ctttggcgac gaaatcgagc gtatcgtcga ggtcgacccg
      421 ctcaccggcg agctcctgat ccaacggacg gagatcgaca tctaccccgc caagcacttt
      481 gtgaccactc aggaccgaat gggccgcgcg atcgaggaca ttcaggacga actggcggag
      541 cgcgtgaagt tcctcgagga gaatggccag gtgctggagg ccgcgcgcct caagcagcgc
      601 acgatgtacg acatcgagat gatgcaggag gcgagctact gcgcgggcat tgagaactac
      661 tccatgcatc tctcaggaag gaaaccgggc gaccagccct cgacgctgct cgactacttc
      721 ccggacgact tcctgctctt cgtcgacgag tcgcacatca ccctgcctca ggtacgaggc
      781 atgttcggcg gggaccgttc ccggaaggat gtgctcgtcg agcacgggtt ccggctgcca
      841 tcggcgcgcg agaaccgccc gctcaccttc gaggaattcg agcggacgat cggacaggcc
      901 atcttcgtct ccgcgactcc gggcccctat gagtacgagc actccagccg cgttgtcgag
      961 caggtcattc gcccgaccgg gctcgtcgac ccggcgatca ccgtcaagcc gaccaagggc
     1021 caggtcgacg atctgctcgg cgagatcaac cagcgtgtcg cgaagggcga gcgaacactc
     1081 gtcacgacac tgaccaaacg catggcggag gacctctccg actacctcaa ggagatgggg
     1141 atccgtaccc actacctgca cagcgagatc gacactctgg aacggatcga gattcttcgc
     1201 gatctgcgcc tcggcatcca cgatgtcgtc gtgggcatca acctcctgcg cgaaggcctc
     1261 gacctgccgg aggtgtcgct cgtcgcgatc ctcgacgcag acaaggaggg ctacctgcgc
     1321 tccgagggat cgctcattca gacgatcggc cgcgccgccc gtcacgtcga cggccacgtc
     1381 atcatgtacg cggacaccat gacccgctcg atgagggccg cgatcgacga gacctaccgc
     1441 cgccgcgaga agcagatcgc ctacaacgag gagcacggca ttgagccgcg cggcatcgtc
     1501 aaggcaatca aggatctcac cgaccgcgtg cgcgaagtgg cagaacagac ggcttcgtac
     1561 gacactggtg gtggaccggc ggagggcgtc attcatcagc tgccgaagga cgagctgacc
     1621 cggatgatca aggacctcga gtcgcagatg aagaaggccg cgcgcgatct cgaattcgaa
     1681 aaggccgccc ttctacgcga ccaagttgtc gagctccgcc gcgtgatgga agccgatccc
     1741 atcgaggcat gattcgttgc agacagggag acattaccgt gtatcaccgt ggctttgccc
     1801 gaacggcgct cgttgcgttg ctcaccgtgc tgctgctcgc catgccgagc ctgtccgtcg
     1861 cgcagggcgg caccccttcc ccgggcgacg aatcggagct ggccccccag ctacccaccg
     1921 tcgacttgcc aaccttgaat gagatgaact accggttcga gctcgagtcg gtctggaccg
     1981 gtagcgccgg cacgccggcg gagctgcctg tttacctgtt cgagcccatc gtctacaccg
     2041 aggaggatgt cgcgcggatc gcggaggaac tgggggtcga gggcgagatc acctcacagg
     2101 gcgcgggatc gtactcggta tctggcaacg gaagtatctt cacgtcgccg gggctgctgc
     2161 agtatgtctc tgcggctgag ccgcccgacg aggacctgcc gagcaacgca gaggcagtgg
     2221 ctctcgccca cgaatggctc cgcaccaccg ggctgctgcc agccaacatc ggcgagggcg
     2281 aggtgctggc aaccattgag acgccggcgc ggaagatcgt cggattccag ccggcgacac
     2341 catcaccgtt gctcagttcg accccaggca tcaccgtcac catcggtccg gatgccacgg
     2401 taatcgaggc gcgcatcaac tgggccgaga tcaccgagag cgatgtctat ctgctgcgac
     2461 cggtggagga cgccttcgca caggttgcct cgcgacagtc gtacatcaac gccacgctgc
     2521 ccgccgatca gttcccacag ggcagcacca tttccggcac cgcaacctat gactccgtat
     2581 cgatcgcata cgcgagctcg ggggttcccg gcgagacgca gttcctgcag ccggtctatg
     2641 tcttcgaagg atcggtcgat gttgccgagg ccgagggaac cttcgacatc gtcgcctatg
     2701 tgcccgccat cgtcaccgga ctcgaaccgg tcggatagcc gcactagggg aatgacggcg
     2761 atcatgaagc tcctgcttgt gaatggaccg aacctcaacc tgctcggcac ccgcgaaccg
     2821 gaggtctatg gccaccagac cttggcggac atcgtcgatg ccgtccgaag ggaggccgca
     2881 gcgcacgact gcgacctcct cgatttccaa tcgaatcacg agggagccat tctcgacttc
     2941 attcacgaga acgcggccag cgcggacggt ctgatcatca accccggtgc actgacgcac
     3001 tacagcattg ccctgcgcga cgcgatcagt ggcaccggca tcgccaccat cgaagtccat
     3061 ctctccaatg tccacacgcg cgaggcgttt cggcaccact cggcgatctc ccccgtcgcg
     3121 gctggtacca tcgtcgggct tggtgcgcac ggctaccatc tcgcgcttca gtggttccgt
     3181 cgaacgatac agccagtaca gaaaggctcc tgatgagcgt catcgctcgc ctgaagaatg
     3241 cccggcaggt catccgcgac accgggctcg atgccattgt cgtcaccaac cccaccaacc
     3301 gtcgctacct gacggggttc accgccgagg accacgccgc cgacgagtcg gccggcgtgg
     3361 tcctgctcac cgcggagaac gccaccctca tcgtctcgcc aacgaacctg ccgtgggcag
     3421 ctgccgaggc ggatgcggaa tcgatcaccg tcaagccaca caatggtgac atgcctggaa
     3481 ccattgccga gcggctgacc ggcatcgacg cgaccaccgt tgccgtcgag gacctcacta
     3541 cccccacggc ggtctggttt gcgctgcggg cgtcgctcgg cgagagggtc gagatggtcc
     3601 gcgccagcga ctgcattgat cagttccgcg cgatcaagac tgctgacgaa ctcgagcacc
     3661 tgcgcgaggc tgcgcgacta accgacacgg ccttcgccat cgccgtggat cgacttcgga
     3721 aaggcgtaac tgagcgggaa gctgcagacg tggtgcgtga tgccctgcgc gaggtcggca
     3781 gcgagggcga ggcgttcgag acgatcgtct ccagcgggcc gaatgctgcc aagccgcacc
     3841 accggccggg agcacggaga ctcgctgccg gcgagccgat cgtcatcgac atgggcgcca
     3901 gggtgagcgg ctataatggg gacctgacgc gcaccgttag tctcggcaag gcagacgaaa
     3961 agcttggtag gatatacggc gttgtcctcg aggcgcagct cgccggactg gacgcgattc
     4021 gagccggggt gagtgccgca cgcgtcgacc aagccacccg cgaggccttc gccgcacacg
     4081 gcctcgagca gcacgtcgtc cacagcgccg gacatggtct tggccttcgg gtccacgagg
     4141 ccccgtcggt gcgcaagaca tccgaggaga cgctggacgc cgggcacgtg atcaccatgg
     4201 agccggggct ctacatcgag ggctggggcg gggtccgcat cgaggacgtc gtcatcgtcc
     4261 gcaacaccgg ccacgagaac atcaccacgg cgccgaaggg gctcgactcg ctcgagcact
     4321 gacaaggagg acaccttaca ttgatgatcg atacaggtga tatccgcaag ggcagcacgt
     4381 ttgagcagga cggccgcctt ctcaaagtcg tcgacttctc acacaacaag cagggccgcg
     4441 gctcggcaca actgcgcatg acgctccgcg acctgcgtac cggctcgctc acacagcaaa
     4501 cagtcatggc cggtaccaag ttcaactcgg tacgcctgga gcgctcgcac gtgcagttcc
     4561 tctaccgcga gggcgacgct tttcacttca tggataccga aacgttcgac cagatcctgg
     4621 tcatggagtc gaccatcggc gacgcggccc agtacatcaa ggagaacgat gtgatcgacg
     4681 ttctcacgta tgaggacgag cccatcgaca tcgagcttcc cacctccgtc aacctggcag
     4741 tcacgcaaac cgacccggga ttcaagggcg acaccgcgac cggtggctcc aagccggcca
     4801 cactcgagac cggcctgacg gtcaacgttc cgctctttgt cagcgagggg gaccagatca
     4861 aggttgatac gcgcaccggc gagtacatcg agcgcgttgg ataaagggga acgaggatgg
     4921 agcgcttcgg cggcatgaac cggacagacc aggaagcctc atccacacaa gaactgatcg
     4981 gcgcggtccg cgagctgatc tccatgatgg gcaagggcgg catctccgag ctcgacctga
     5041 catcaggaga cgtctccatc cggctccgcg gcggcacggc cgctgtggcc tcccccgctc
     5101 cgtccgtgat gcccaacggc gagatggacg atcccgaggc cgccgaggac gagggtcacc
     5161 tcatcaccgc tccgatgatc ggtaccttct atgccgcccc ctcgcccggc gaggagccct
     5221 tcgtgcaggt cggggacgaa gtcgaggttg ggcaggttgt tggcatcatc gaagcaatga
     5281 agatcatgaa cgagatcgtc gccgacaggc ccggcatggt cgccgagatc ctcgtcgaga
     5341 acgcgcaggc ggtcgaatac ggatcgcccc tcgtgcggct cgctgagcag accgacgagc
     5401 tctgatgacc tcccacatcc tcccggtcag caccctcaac gcctatatcc agcaggtcgt
     5461 ctccgccgac gacatcctcg ccgatgtctg gatcgagggc gagatcagcc agctgaccaa
     5521 ggcgcgctcg ggacacgtct acttcaagct ctgcgagagc gatgccctga tcgactgcgc
     5581 gatgtggaag cacagcgcag cccggcagac cttcatcccg cgcgtcggcg acgaggtcat
     5641 catccacggc cacgccgatt tctacccgcc tcaagggcgg ttgcagatca ttgccgatgt
     5701 cttcgagcac cagggccagg gaattctcgc gctcgagatg gagcgcctgc gccagaagct
     5761 cgaggcggaa gggctcttcg atctcgcacg caagcgggag ctgcccccgt ttccgcgccg
     5821 gatcggcgtg gttacgagca gcgccggtgc tgtttggcat gatattcaga ccgtcacccg
     5881 ccggcggttc cctctggtgg agctggtgtt gatctcggcg tcggtgcagg gcagcaacgc
     5941 accgcgtgag atcgtcgcgg ctattcgcca gatgtgcgag ctcggcaata tcgacgtgat
     6001 gatcgtcggt cgtggaggcg ggtcacccga agacctcgcg tgcttcaaca gtgaagaggt
     6061 ggcccgggcg atctttgcca gcacggtacc agtcgtttcc gcggtggggc acgagaccga
     6121 cgtctctatc gccgacatgg tcgccgacgt ccgcgctgcg acgccatccg ccgccgccga
     6181 gatcgtcctt cccgacattc gggagctggc cgtacttctc gctgacacgc gggaggaact
     6241 gatcgaaatc gcggagcggc ggctcgccaa cgaacggtcc cggctcggcg cgctcacgcg
     6301 ccgtttccac cagcaatcgc cgcgggcgca catctcacgc acggagcagg aactcgccac
     6361 actggagcgc cggctccggc tctccgggcg gagctatctg cagcagcggg aaacgtcgct
     6421 ccggagcacg gcgaaggtcc ttgccgcgct ggatccaacc cgtgttctcg aacgcgggta
     6481 tgcagtgatc gcaacgcccg cacccccctc gccgatccgt cggatggcgg atctggacgg
     6541 cgacacggca gtggtagtca acttccggga tggaactgcc cgcggcacga tcgccccgca
     6601 caccaacgat tcacaggagg atgcgcagca gtcatgaacg ccaccgatac atctgcactg
     6661 gaa
//
LOCUS       DASYWL010000060         4314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_246752, whole genome shotgun sequence.
ACCESSION   DASYWL010000060 DASYWL010000000
VERSION     DASYWL010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246752"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="VGR22_02050"
     CDS             <1..991
                     /locus_tag="VGR22_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEV2127381.1"
                     /translation="GGPMNALNQFATATPVRAGGTGLLDDFAVVTAIGNTPLLPLRRL
                     AAHAGLPETVELWLKAEWTNPGGSIKDRPALGIVCAALASGELGPSRILLDATSGNTG
                     IAYAMLGAALGFPVELILPGSASDERKRILAAYGATVILSDPYEGSNGAIRAARELAA
                     NAPDRYFYADQYNNPANPAAHVATTGPEIWSQTGGRVTHFVAGLGTTGTLMGAGRYLK
                     ARNQRIRLIGVQPSESFHGIEGLKHLPTAILPGIYDSGVPDIQKEVSTEEAFDLVRTL
                     ARVEGLFAGTSTGATLGAAIGVGHALAARDERAVLVAVAPDGGSKYLSTGLWA"
     gene            1062..1370
                     /locus_tag="VGR22_02055"
     CDS             1062..1370
                     /locus_tag="VGR22_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HEV2127382.1"
                     /translation="MAEDESIQVLIPTPLRRFTDGQARVSGRGGTVGELLTELNIRYP
                     GLKERICEEDGDIRRFVNVFVNGENVRERDGAATAVKPGDEVGIIPAMAGGTTAARRA
                     "
     gene            1376..1816
                     /locus_tag="VGR22_02060"
     CDS             1376..1816
                     /locus_tag="VGR22_02060"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEV2127383.1"
                     /translation="MSSDIHLPAAMRDEIVAHARAEAPRECCGVIAGQDGRPIQLYRL
                     TNLERGTDLYRIDDTELYRVYRELDKRGCEILAIYHSHPVSPAYPSRTDVDLAFWSEA
                     FYLICSLAVPGSPDIRAFRIVDERISEVEIMPKPGRSGVTNDGR"
     gene            complement(1881..3065)
                     /gene="moeB"
                     /locus_tag="VGR22_02065"
     CDS             complement(1881..3065)
                     /gene="moeB"
                     /locus_tag="VGR22_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEV2127384.1"
                     /translation="MSAIYRDLFDRTKAQIREVDVPGARDLIDAAGAAVLDVREQDEV
                     DQGVVPGAVHVPRGYLEMRVEETIPDKAQPIIVYCAGGVRSAFAAKSLQDLGYNDVVS
                     LAGGFTAWKGAGEPWEMPQTLTPDQRRRYSRHLLIPEVGEVGQQRLLHAKVLLVGAGG
                     LGSPAALYLAAAGVGTLGVVDADTVDDSNLQRQVIHSTDRIGMPKVESARLTIEALNP
                     DVTVICHEERLTKENVLEIVAPYDIILDGTDNFATRYLINDACVLLGKPNVHGSIFRF
                     EGQATTFITGEGPCYRCLFPNPPPPELAPSCAEAGVLGLLPGTVGLIQATEVAKLILG
                     VGEPLVGRLVTYDALTTTFRDFKFQRDPACPMCGSAAPTTLDDIEYSDIACAIPPLTT
                     AS"
     gene            complement(3545..>4314)
                     /locus_tag="VGR22_02070"
     CDS             complement(3545..>4314)
                     /locus_tag="VGR22_02070"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEV2127385.1"
                     /translation="FYAGGIPAVLRQLLPLLHGDALTVTGKTLAQNVEDAEIQDHDVI
                     RPLDNPLQLEGGTAVLWGNLAPDGAVIKHAAATPHLLRHQGRAVVFRDLEDLRARIDM
                     PDLEVAEEDVLVLQNVGPVGGLGMPEVGNFPIPGKLLRQGVRDMVRISDARMSGTAFG
                     SIVLHVAPESAIGGPLALVRDGDRIELDVPARRLHLHVDDAELATRRAAWTGPSPAFT
                     RGYGRLYLEHVTQAPLGCDFDVLRGNDPVGATHQPKF"
BASE COUNT          788 a         1335 c         1434 g          757 t
ORIGIN      
        1 aggtggtccc atgaatgcat tgaaccagtt tgccaccgcg acgcccgttc gcgcgggtgg
       61 cacgggtctg ctcgatgatt ttgccgtcgt gacagccatc ggtaatacgc cactgctgcc
      121 gctccggcgg cttgcagcgc atgctgggct gccggagacg gttgaactct ggctgaaggc
      181 cgaatggacc aaccccggcg gctcgatcaa ggaccggcct gcgcttggaa tcgtgtgcgc
      241 ggcgctggcc tccggcgaac tagggccgag tcggattctg ctcgacgcca cgtccggtaa
      301 caccgggatc gcgtatgcga tgctcggggc ggcgctcggg tttccggtcg agttaattct
      361 gccaggcagc gcctccgatg aacgaaagcg aatcctcgcg gcttatggtg cgaccgtaat
      421 tcttagtgat ccctatgagg gctcgaacgg cgcgatccgg gcggcacgtg aactcgcggc
      481 taacgcaccg gatcgctact tctacgccga tcagtacaat aacccggcca accccgcggc
      541 gcatgtcgct accaccggcc cggagatctg gtcccagacg ggcggtcggg tgacgcattt
      601 cgttgctggt ctcggcacga cggggacgct gatgggtgcg ggtcggtacc tgaaagcgcg
      661 taatcaacgg atccggctga tcggggtgca gccgtccgag tccttccacg gcatcgaggg
      721 tctaaagcac ctgccaacag ctatcctgcc cgggatttat gactccggcg tacctgatat
      781 ccagaaggag gtctcgaccg aagaggcgtt cgatctcgta cgcaccctgg cgcgggtcga
      841 gggtctgttc gccggcacct cgacgggggc gacgctgggc gcggcgatcg gggtcggaca
      901 cgcgctggcg gcacgcgatg agcgcgccgt gctcgtcgcg gtcgcgccgg acggtgggag
      961 caagtatctt tccacggggt tgtgggcgta acgcaggtac cagccaccga ttcacaacca
     1021 aaatctgata gctgatggct tcttttggag gactgttcac gatggccgag gacgaatcga
     1081 ttcaggtgtt gattccgacg ccactgcggc ggtttacgga cggacaggcg cgcgtgagtg
     1141 ggcgcggcgg cacggtcggc gaactgttga ccgagttgaa tattcggtat cccggcctca
     1201 aggagcgcat ctgcgaggag gatggggaca tccgccggtt cgtcaatgtc ttcgtcaacg
     1261 gcgagaacgt gcgggagcgc gacggtgcgg cgacggcggt gaaaccgggc gacgaggtcg
     1321 gaatcatccc ggcgatggct gggggcacca cagccgctcg tcgcgcatga gcgacatgag
     1381 ctcggacatc catctgccgg ccgcgatgcg agacgagatc gtggctcacg cgcgggccga
     1441 ggcgccgcgc gagtgctgcg gcgtgatcgc cggtcaggat ggccgaccga tccaactcta
     1501 ccggttgacc aacctcgaac ggggcaccga cctctaccgg atcgacgaca ccgagctcta
     1561 tcgcgtctac cgcgagttgg acaagcgggg ttgcgagatc ctcgcaatct accactcgca
     1621 ccccgtctcg ccggcgtacc cctccaggac cgatgtcgac ctcgcctttt ggtcggaggc
     1681 gttctacctc atttgctcgc ttgcggtgcc cggctcgccg gacatccgcg ccttccgcat
     1741 cgtcgacgag cgaatcagcg aggttgaaat catgccaaag ccaggcagat ctggcgtcac
     1801 gaacgatggt cggtgacgcg ctggcgtgtc ggcatccgtc atgcacagtt gccgatcatc
     1861 ctgaatagca gccgaattcc tcaactcgcc gtcgtgaggg gtgggatggc gcaggcgatg
     1921 tcgctgtact cgatgtcatc cagggtggtc ggagcggcgg aaccgcacat cgggcaggcg
     1981 gggtcacgct ggaacttgaa atcgcggaag gtggttgtga gggcatcgta ggttacgagg
     2041 cgaccgacga gtggctcgcc gacgccgagg atcagcttgg cgacctcagt ggcctggatc
     2101 aggccgactg tgccagggag caaaccgagg acgcccgctt cggcacagct cggagctagc
     2161 tccggcggcg gcggattcgg gaagaggcag cggtagcagg gaccttcacc agtgatgaag
     2221 gttgtcgcct gaccctcgaa gcgaaagatg ctgccgtgga cgttcggctt gccgagcagg
     2281 acgcaggcat cgttgatcaa gtagcgggtc gcgaagttgt cggtgccatc gaggatgatg
     2341 tcgtagggcg caacgatctc caggacgttc tccttggtca accgctcctc gtggcaaata
     2401 accgttacgt ccggattgag cgcctcaatc gtgagtcggg cggattcaac cttaggcatg
     2461 ccgatgcgat cggttgagtg aatgacctgg cgctggagat tactgtcgtc aaccgtgtcg
     2521 gcatcgacca ctccgagggt gccgacgccg gctgcggcta gatagagcgc agccggcgag
     2581 ccgagcccgc ccgcgccgac gagaagcacc ttggcgtgga ggagcctctg ctgcccgacc
     2641 tcaccaacct cggggatcag cagatggcga ctgtagcgac gacgctgatc cggggtgagg
     2701 gtttgcggca tctcccacgg ctcgccggcc cccttccacg cggtgaagcc cccggcaaga
     2761 gagacgacat cgttgtagcc gagatcctgg agggacttgg cggcgaaggc ggagcggacc
     2821 ccaccggcac agtagacgat gatcggctgc gctttgtcgg ggatggtctc ctccacgcgc
     2881 atttcaaggt acccgcgcgg gacgtggaca gcgcctggga cgacgccttg atcgacctca
     2941 tcctgctcgc gaacatcgag cacggcagcg cccgcggcgt caatgagatc acgcgcgccg
     3001 gggacgtcaa cctcacggat ctgcgccttg gttcggtcga acaggtcccg atagatggcg
     3061 gacatgcgtt atggctccat gctgggcggc acgactatca agggagcgcc gaatgaactg
     3121 agttcttcgc gaagggtatg ggaatagcaa tgccggtgca agaggcgcca ggctattgta
     3181 ccgaaaggag gtgtcggctt tccttcccgt gcgacgatat gtgggaattg agcccttttc
     3241 cgtccatcag tagaaaggac gcggaggagc gtgcctgtag ccaagccgac caaggacggg
     3301 acgtcgatca agtgttccac gcatggcacg cgcgccttta ggaggctcgc gagggatttc
     3361 cacccgcgcc gcccctgacg acctcggcac ctcacttgac ccgttgacgt cgatccgcag
     3421 tggtggtgca gacaatgcca tagcgcgctc tccgatcgtg gtacgtgctc gtgaacgccg
     3481 atgcccgagc cgggtttacc gattgtggct accgcgacag cggacaccca tcaggagaac
     3541 cactctagaa cttgggttgg tgagtcgccc ccacgggatc attgcctcgc agcacatcga
     3601 agtcgcagcc cagaggtgcc tgagtgacgt gctcgaggta gagccgcccg tagcctcgag
     3661 tgaacgccgg cgatggtccg gtccacgccg cgcgccgcgt cgccaactca gcatcatcaa
     3721 cgtgcagatg caagcgccgc gcgggcacat caagctcgat ccggtcgccg tcgcgcacca
     3781 gcgccagcgg cccaccgatc gcgctctcgg gcgcgacgtg gagcacaatg ctcccgaacg
     3841 cggtgccgct catccgcgca tcagagatcc gaaccatgtc gcgtacaccc tgccgcagga
     3901 gcttgcccgg gatcgggaag tttcccactt ccggcatccc caacccgccg accggcccga
     3961 cgttctgcag gaccagcacg tcctcctcgg ctacttcaag atcgggcatg tcgatccgtg
     4021 cgcgtaagtc ctccaggtcg cggaacacga ccgctcgacc ctggtgccgg agcaggtggg
     4081 gcgtcgcggc ggcgtgtttg atcaccgcac cgtcgggtgc gagattgccc cacaagacgg
     4141 cggtgccgcc ctcgagctgc agaggattat ccaacggtcg gatcacgtcg tgatcctgga
     4201 tctcggcgtc ctccacgttc tgcgccaagg tcttgcccgt gacggtcaac gcgtcaccgt
     4261 gcagcagcgg caggagctgt ctgagaacgg ccgggatccc accggcatag aaga
//
LOCUS       DASYWL010000061        11479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_21140, whole genome shotgun sequence.
ACCESSION   DASYWL010000061 DASYWL010000000
VERSION     DASYWL010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 11479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11479
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21140"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..159
                     /locus_tag="VGR22_02075"
     CDS             13..159
                     /locus_tag="VGR22_02075"
                     /inference="COORDINATES: protein motif:HMM:NF025873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEV2127386.1"
                     /translation="MPGVGPKRKKALIRKFGSVKGIREASAEELTTVDGINAQLAENI
                     KANL"
     gene            257..907
                     /locus_tag="VGR22_02080"
     CDS             257..907
                     /locus_tag="VGR22_02080"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEV2127387.1"
                     /translation="MKVLSLEAPRALEETIHTVQHGGVVAFPTDTVYGIGASLRHPDG
                     LKRIYKIKGRDMVKPLPVLLSSIDHIDLVAEVPHPHILALLRHFWPGGLTVALPAKPD
                     IPDEVTHADGSVGVRVPDHSIALALCDRAGGALATTSANRSGEPPACSADDVIAAFGD
                     RIDMVLRGGYTPGGEPSTVIRVEDDATIAVIRAGSIESEEVENVWARIQAETDSPA"
     gene            962..1909
                     /locus_tag="VGR22_02085"
     CDS             962..1909
                     /locus_tag="VGR22_02085"
                     /inference="COORDINATES: protein motif:HMM:NF040134.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127388.1"
                     /translation="MTDNERRHPLRSRPPQEPPEVDESQEDDTHHKIFGLGIPRAESR
                     QPAYGQGVVVSLLTAGAVLILAIITVGNDAEGDPPVFESTSTVFWIVAVVVMVLAATG
                     AQFAERTATFAAEKVGQPRLRTTMATAWTVPLVATFAAIVLAATFHNQLMLLVSPLIA
                     FIGTAGALLSRDLLDEVDEQSSRVASTIHTLVVHGVAFFAFSAVYLNKLDIWLAGPLI
                     AIIAFLLTLETLERAGVAPPRRIFFSLASGWVLFQSMFALDWWPTYGWAGGAVLLAVF
                     YIVAGLILVYCESGRVQQRDYVEFGAVGGIAILLLALLG"
     gene            1931..2137
                     /locus_tag="VGR22_02090"
     CDS             1931..2137
                     /locus_tag="VGR22_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127389.1"
                     /translation="MSDPIDAFRPRSDSKPPETTEEWMNELERLETMLEEMHEQGIED
                     RSELERRISEIEAALEAADDEPGE"
     gene            2134..3489
                     /locus_tag="VGR22_02095"
     CDS             2134..3489
                     /locus_tag="VGR22_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEV2127390.1"
                     /translation="MKDLFDLQRDQHLANRAPLATRVRPQTLDDLVGHEELVGPGSLL
                     RCAIESDRLSSLILWGPPGSGKTTLARIIANTTNAAFESVSAVTSGVADLRAVIASAK
                     ERLGMQGTRTVLFIDEIHRFNKAQQDAILPHVEDGTVILIGATTENPSFEVNAPLLSR
                     SRVVTLQSLSDGDIDRVLDRAMQDPEKGFGQQNLTLTPEGRSLLVNLANGDARFALNT
                     LEYAATGVEPGGTIDAELVSEAAQRRAATYDKSGEGHFDTISALHKTIRGSDPDAAVY
                     WLARMLERGDDPLYVARRLVRAASEDIGLADPNALQKAIAAQQSVHFLGMPEGGLALA
                     ELVVYLALAPKSNAVYKAWNAARQDVAETRNDPVPLHLRNAPTKLMRESEYGRGYRYA
                     HDYDGGVVGQQNLPENLEGRRYYEPTDRGFEQELGERLQEVRAIYDRTRADATSEEDV
                     E"
     gene            3486..4229
                     /gene="rph"
                     /locus_tag="VGR22_02100"
     CDS             3486..4229
                     /gene="rph"
                     /locus_tag="VGR22_02100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEV2127391.1"
                     /translation="MTIEIPVKRSYGRTVEDLRPVSIMPNALPHAEGSAYIKIGDTHV
                     ICSATVEERVPGWMKGTGRGWVTAEYGMLPRATGQRTEREATRGSQKGRTVEIQRLIG
                     RALRAGVSLEKLGERQIILDCDVIRADGGTRCASITGAWVALAMACRRLMDEKRLRSY
                     PITSQIAAVSVGVVKGVPMVDLDYREDSRADVDVNVVMTEKSQFVEIQGTAEAAPFSS
                     GTLDEMLVLASTALDQLFSRQLEAIGGDR"
     gene            complement(4304..5335)
                     /locus_tag="VGR22_02105"
     CDS             complement(4304..5335)
                     /locus_tag="VGR22_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV2127392.1"
                     /translation="MSRIQGTNLGSVRQSDGAIRLHRLENPYAPTDAVAEALADCEPV
                     SETADEMAADIRRRLGRIAGVDERWVVLANGIDQLYEGLVQWQVDSGPFAVFPPTQLG
                     ELQRVLDKGGDVEVASRTEVFELGLSTTARRFPLNSISVVLSPNDPTGTLVDLHQLVR
                     LSRQSRLAVIDERHGAYTPRSTAPFAREFDNVVVLQSMEWWAGLRETPLAWAICPPSL
                     GNALQEAMGDPEIHRPALIAARATLDDWSWLQGTLRWVSMEKGRLFRQLRKLNMLYTP
                     YPSWANFVLTRFARGSVDFFVPRLEERGIVVHVPDQDLLPDHIRVSAVSAETTDALKR
                     ALIDIALDL"
     gene            complement(5332..6336)
                     /locus_tag="VGR22_02110"
     CDS             complement(5332..6336)
                     /locus_tag="VGR22_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV2127393.1"
                     /translation="MLPLARLASACGRRLGTPVYVERPCRGEGTGNTRYTRRSLRTAG
                     TVRMTLRHQSIEVGLTAGEVQRIRVMAVGDVHLRADAPMSVQEGLRLAADEVDLVLLT
                     GDMTDNGRKIEVEQVARVMAGVDAPVYAVMGNHDRRTLRRKEFRRALATGGVELLDGE
                     STVVESRHGLRVGLVGIGGYGGGFWPDEAPDLLSTRFSQAVAVRARREAARLEAALDA
                     LASHDTDLVIVTMHYAPTTTTLGAEPMMKHWMLGNSVLGRVVDRHDVALVLHGHAHLG
                     NYHGVTPGGTPVRNVALPVVGAPVVLEVGRDGVVDRYEAGEQRAARMMAASRDRSRIP
                     "
     gene            6306..7544
                     /locus_tag="VGR22_02115"
     CDS             6306..7544
                     /locus_tag="VGR22_02115"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEV2127394.1"
                     /translation="MHWPTWPAATWAAHILAGRESLTVTTPTGTGAWRPALVLTIVIA
                     VLSALWVPTQPAAAAIQVPDVSARGVYAFDLDTGIQLFAKNEHERMPIGSVVKVVTAL
                     VTVEHTELSDKVMIVADDLVDDPLYSNMQLQAGDTLTVSQLLYGLMIPSGSDAATALA
                     RYVGGILSRSDDPIAQLDAFVAEMNAFVDRHGLENSRFTNPHGLDAPNNYSSAHDISI
                     LSGMLMKNEFLAKVVREPGYSFISAGPEARQYQASTTNQLLGEAGVIGIKTGSTDDAG
                     GCVVLARQVNNANSTVITAVLGAELAYDASGMITTDERWADASQLLQFMDTSFAWLPL
                     ADAETFVNLPTELAVWDVQIAGNPTIPVAVREDTQTRYQLALNPDGGGTIDIYYDQVR
                     VGSLPLEAASATSGNVAIAA"
     gene            7541..8545
                     /locus_tag="VGR22_02120"
     CDS             7541..8545
                     /locus_tag="VGR22_02120"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEV2127395.1"
                     /translation="MSDRRRDDEHESIREYQGERLQRVLSQRGVASRRAAEKMMLDGR
                     VQVNGRVVTELGTRVRPNRDEIRVDGKVLRPQPPRYVMLNKPTGFITTVSDERDRWTV
                     MDLVDVRERVYPVGRLDRNTQGLLLLTNDGDLAHRVMHPSYRVDKEYLAFTDQRPTET
                     QLQRLRQGIDVDGYKVVPEDARLFRETSEGVAIRIVIHEGLYHEVRRMMEAVGIEITR
                     LRRTRLGPLRLQGIPSGAWRDLTPGELVQLYQAVDLAEEAADHINSRRTIVREPVGGF
                     THKGAGASGQQRGPGLRGERKGEPTETRQGSRSEGRMGRNDNVRSSQSHRNRRPRRRG
                     "
     gene            8553..9200
                     /gene="cmk"
                     /locus_tag="VGR22_02125"
     CDS             8553..9200
                     /gene="cmk"
                     /locus_tag="VGR22_02125"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEV2127396.1"
                     /translation="MAAEVADELDSLLFDTGALYRVVTLLALRTGTPIADGDRLAALA
                     SEATIELCPATVEDGRTSDVLLDGDDVTWEIRRPEIDANVSAVSAHPQVRGALLDLQR
                     EIADGIAAVLVGRDIGTMVIPDAGLKIWLDASVEERARRRYLDMQERGIDMSYSAILE
                     DLRRRDQFDADRPVSPLRRAHDAITIVTDGRDVEDIVHEITELASARQLDTGTAR"
     gene            9197..9964
                     /locus_tag="VGR22_02130"
     CDS             9197..9964
                     /locus_tag="VGR22_02130"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEV2127397.1"
                     /translation="MSDLAGHGAPDPEAGTVRGIWRKLIRIPLYAFIWLVLRVRVEGM
                     DNIPPEGNGVFVVSNHLHNADPVLLEIVFPRPLHFMAKEEIYRIKPLGWLVSKFGNFP
                     VVRGKSDRRAVRSAIARLQHGVAVGLFPEGTRSRTMRLGPARAGAGFIANQANVPILP
                     VAITGSERLPLNGKRPPAVRPVADHPGIFIRIGEPFTLPKTGNDGRRLSPEQAADRMM
                     WAIAELLPEDYRGVYPSSESSTEREASASASSAARTN"
     gene            complement(9892..11304)
                     /gene="obgE"
                     /locus_tag="VGR22_02135"
     CDS             complement(9892..11304)
                     /gene="obgE"
                     /locus_tag="VGR22_02135"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEV2127398.1"
                     /translation="MLVDTARIHVKAGNGGNGSASFRREKYVPKGGPDGGDGGKGGDV
                     LLRVVPNLNTLLPFQYVHLYKAENGGDGAGRNRNGRKGKDTIVKVPPGTVVFLEEAGE
                     QIADLTEPGEELVVARGGKGGLGNVHFKSSTRRAPRVAELGEPGEERRLRLELRLIAD
                     VGLVGLPNAGKSTLLSTVTKAKPKVADYPFTTLQPNLGVVEIGGAGGQTFVMADIPGL
                     IEGAAGGAGLGHEFLRHVQRTKVLIHVLDASGGLEGRYPIEDFQTIMEEIETFDPEMG
                     EKPMFVALNKIDLQESRDNVDRLRSWLIEQGYERIYEISAATGEGTDPLVQDVAAQLR
                     EILEREAEQKRSAEEQPKRRVYTLGDVDERAWDVERLSKHHFRVLGVGIERFTRMTNF
                     DVPDSVDRFQRVLEGSGIQAELERQGVQEGDTVHIADFEMPWGEQDEEGNVLSADQFV
                     RAALEAEAEASRSVDDSDEG"
BASE COUNT         2086 a         3868 c         3592 g         1933 t
ORIGIN      
        1 aagctggacg acattcctgg cgtcggaccg aagcgcaaga aagcgctcat ccggaagttc
       61 ggatcggtga agggcatccg ggaggcatcg gcggaggagc tgacgacggt tgacggcatc
      121 aacgcgcagc tcgccgagaa catcaaggcg aatttgtaag ggcccgcgaa accatgcagg
      181 cggccctcgt gtccgctcgc cgcgggcgaa gcgcgcattg gtagtcgcgc tggtcgcctc
      241 aggccaagga ggagtggtga aggtactgtc gctggaagcc cctcgtgcac ttgaagagac
      301 gatccatacc gtccagcacg ggggcgtggt cgccttcccg accgataccg tctacggcat
      361 cggtgcgtca ctgcggcacc cggacgggtt gaagcgcatc tacaagatca aggggcgcga
      421 tatggtcaaa ccgctccctg tgctgctctc ttcgatcgac cacatcgacc tggtcgccga
      481 ggtaccgcac ccgcacatac tcgccctgct ccggcacttc tggcccggtg gcctgacggt
      541 cgcgctcccc gcgaagccgg acattcccga cgaggtaacc cacgccgatg gctccgtcgg
      601 cgtccgagtg ccggaccact ctattgccct cgctctctgc gaccgtgctg gcggtgcgct
      661 ggcaacaacg agcgccaacc gctccggaga gccgcccgcc tgcagcgcgg acgacgtcat
      721 cgctgcgttc ggcgacagga tcgacatggt gctccgtggc ggatacacgc cgggcggcga
      781 gccctccacc gttattcgcg tcgaggatga tgctacgatt gcggtgattc gtgccggatc
      841 catcgagtcc gaagaggtcg aaaacgtctg ggctcgcatt caggcggaga ccgactcgcc
      901 agcctgaagg cacgagccta cttgaaccgc gtctagtgcc agcccgagca caggggtccg
      961 tatgaccgat aacgagcgca ggcatccgct acgatcccgc ccgccacaag agccacccga
     1021 ggtcgatgag tcgcaggagg acgacactca ccacaagatc ttcggcctcg gcattccccg
     1081 cgccgagtcc cgccagccgg cttacggcca gggcgtggtg gtcagcttgc tgacggccgg
     1141 cgcggtgctg atcctcgcga tcatcaccgt cggaaacgat gccgagggcg acccgcccgt
     1201 cttcgaaagc acgtcgaccg tcttctggat cgtcgccgtc gtcgtcatgg tgctcgcggc
     1261 aaccggcgca cagttcgctg agcgcaccgc caccttcgcc gccgagaaag tcggtcagcc
     1321 gcgcctgcgc accaccatgg cgaccgcctg gaccgtccca ctcgtcgcaa cctttgccgc
     1381 aatcgtgctt gccgccacat tccacaacca actcatgctg ctggtcagcc ccctcatcgc
     1441 gttcatcggc acggcgggcg ccctgctctc gcgcgacctt ctcgatgagg tcgacgaaca
     1501 gtcgagccgc gtcgcctcga ccatccatac cctcgtggtg catggcgtgg cgttcttcgc
     1561 cttcagcgcc gtctatctca acaagctcga tatctggctc gccggtcccc tgatcgccat
     1621 catcgctttc ctgctgacgc tcgagacgct cgagcgtgcc ggtgtcgcgc cgccacggcg
     1681 catcttcttc tcactggcca gcggctgggt gctcttccag tcgatgttcg cgctcgactg
     1741 gtggccgaca tatggctggg ccggtggtgc ggtactgctg gcggtcttct acatcgtcgc
     1801 aggcctgatt ctcgtctact gtgagagtgg cagggtccag caacgtgact acgtcgagtt
     1861 tggcgcagtc ggcgggattg ccatcctgct gcttgccctg ctgggatagt cggtcagcaa
     1921 ggaggggacc atgtctgacc ccatcgacgc gtttcgaccc cgatcggatt ccaaaccgcc
     1981 ggaaaccacg gaggaatgga tgaacgaact cgaacgccta gaaacgatgc tcgaggagat
     2041 gcacgagcag ggcatcgagg acagaagcga actcgagcga cgcatcagcg agatcgaagc
     2101 agctctggag gctgcagatg acgagccggg cgagtgaagg atctcttcga tctccagcgc
     2161 gatcagcatc tggcgaaccg ggcgccgctc gccactcggg tgcgtccgca gactctggat
     2221 gatctggtcg gtcacgagga gctcgtcgga ccgggctcgc tgctgcgttg tgcgatcgag
     2281 tccgatcgcc tgtcctcgtt gatcctctgg ggcccgcccg gctccggaaa gacaacgctc
     2341 gcccggatca tcgccaacac caccaacgcc gccttcgaga gcgtcagtgc cgtcaccagc
     2401 ggcgtcgcag acctccgtgc cgtcatcgcg tcggcgaagg agcgcctcgg catgcagggc
     2461 acgcgcaccg tgctcttcat cgacgagatt caccgcttca acaaggcgca gcaggacgcc
     2521 attttgcctc acgtcgagga cgggaccgtg atcctgatcg gcgcgacgac cgagaacccg
     2581 tcgttcgagg tcaatgcgcc cttgctctcc cgctcgcgcg tggtgacgct gcaatcgctc
     2641 agcgacgggg acatcgaccg cgtgcttgat cgtgccatgc aggatcccga gaaggggttc
     2701 ggccagcaga acctgacgct gaccccggaa ggacggtcgc tgctggtcaa cctcgcgaac
     2761 ggagacgccc gctttgcgct caacacactg gaatacgccg ccaccggcgt cgagccgggc
     2821 ggcacgatcg acgccgagct cgtctccgag gcggcccagc gccgcgccgc gacatacgac
     2881 aagagcggcg agggccactt cgatactatc tcggcgttgc acaagacgat ccggggctcc
     2941 gatcctgatg ccgcggtcta ctggctcgca cggatgctgg agcgcggtga cgacccgctc
     3001 tacgtcgccc ggcgactggt ccgagccgcg tccgaggata tcggcctcgc agatccgaat
     3061 gcgcttcaga aagcgatcgc ggcgcagcag tcggtacatt tcctcggcat gcctgagggc
     3121 ggactcgccc tcgccgagct ggtcgtctac ctcgctctcg caccgaaaag caacgccgtc
     3181 tacaaggcgt ggaacgcggc gaggcaggat gtcgccgaga cccgcaacga cccggtcccg
     3241 ctccatctgc ggaacgctcc gacgaaactc atgcgtgaat ctgaatatgg tcgtgggtac
     3301 cgctacgccc acgattacga cggcggcgtg gtcggtcagc agaaccttcc ggagaatctt
     3361 gagggtcgac gatactacga gccgacggat cgcgggttcg agcaagagct cggcgagcgc
     3421 cttcaggagg ttcgtgcgat ctacgatcga accagggccg acgccacctc cgaagaggac
     3481 gttgaatgac gattgagatt cccgtgaagc gctcctacgg ccgcaccgtg gaagacctgc
     3541 ggccggtgtc catcatgccg aacgccctgc cccacgccga gggttccgcc tacatcaaga
     3601 tcggcgacac ccacgtcatc tgctcggcga ccgtcgagga gcgcgtgccg ggctggatga
     3661 agggaaccgg tcgtggctgg gtgacggcgg aatacggcat gctgccgcgc gccaccggcc
     3721 agcgcacgga gcgggaggcg acacgcggat cacagaaggg gcgcacggtc gagattcagc
     3781 gtctgatcgg gagggcactg cgagcgggag tcagccttga gaagctcgga gagcgtcaga
     3841 tcatcctcga ctgcgacgtc atccgcgccg atggcggcac ccgctgcgcg tccatcaccg
     3901 gcgcatgggt cgcgctggcg atggcctgcc gccgcctgat ggacgagaag cgcctccgaa
     3961 gctatccgat cacgagccag atcgccgccg tgagcgtcgg tgtcgtgaag ggcgtgccga
     4021 tggtcgacct ggattaccgc gaggattcgc gggcggatgt cgacgtcaac gtggtgatga
     4081 ccgagaaatc gcaattcgtc gagatccagg gcacggccga ggctgcgccg ttctcctcgg
     4141 gcacgctcga cgagatgctt gtcctggcga gcaccgcgct cgaccagctc ttctcacgac
     4201 aactcgaagc catcggcggc gatcgctgac cgggagctgg atcggcacga gcaccaggcc
     4261 gacaacattg gacgcctaca gagaggctta tttcaccctg tcttcacaga tccagagcga
     4321 tgtcgatcag ggcacgcttg agcgcatcgg tcgtctcggc tgagacggca ctgacgcgaa
     4381 tgtggtccgg gagcagatcc tgatcgggga catgaacgac gatcccccgc tcctccagcc
     4441 gcggcacgaa gaagtcgacg gagccacgcg cgaaccgcgt caacacgaaa ttcgcccaag
     4501 acggataggg cgtgtacagc atgttgagct ttcgcagctg tcggaagagc ctccccttct
     4561 ccatgctcac ccaccgcagc gtgccctgca gccatgacca gtcgtcgagc gtggcgcgcg
     4621 ctgcgatcag cgccggacgg tgtatctcag gatcccccat cgcctcctgg agcgcgtttc
     4681 ccaacgacgg cgggcaaatt gcccatgcca ggggcgtctc tcgcagcccg gcccaccact
     4741 ccatcgactg cagcacgacc acgttgtcga attcccgtgc gaacggcgcg gtggatcgtg
     4801 gggtataggc gccatggcgc tcgtcaatca ccgcgagccg gctttgccgc gagagccgca
     4861 caagctgatg gagatcgacg agcgtgccgg tcggatcgtt gggagagagc accacgctga
     4921 ttgaattcaa cgggaagcgg cgggcggtgg ttgacagccc gagctcaaac acctcggtcc
     4981 ttgacgcgac ctccacgtct ccgcccttgt caagcacgcg ctggagttcg cccaactgcg
     5041 tcggcgggaa gacggcgaac ggcccgctgt cgacttgcca ctgcaccaaa ccctcgtaca
     5101 gctggtcgat gccgttggcg agcactaccc agcgctcatc gactccggcg atacgcccga
     5161 gtcgccggcg gatatctgcc gccatctcgt cggccgtctc cgagacgggc tcacaatcag
     5221 ccagcgcctc ggccaccgca tcagtcggcg cgtacgggtt ttccagtctg tggagccgga
     5281 tcgctccgtc cgactgccgc acgcttccga gattcgtccc ctggatacgg ctcatgggat
     5341 tcgcgaccga tctcgcgacg cagccatcat ccttgctgcc cgctgctcgc cggcctcgta
     5401 gcggtcgacg accccgtcac ggccgacctc tagcaccacc ggcgcaccga cgaccggcaa
     5461 tgccacgttg cgtaccgggg tgccacccgg cgtgacgccg tggtagttgc caaggtgcgc
     5521 gtggccgtgg agcaccaggg ccacgtcgtg acggtccacg acacggccca gaaccgagtt
     5581 gcccagcatc caatgcttca tcatgggttc ggcgccgagt gtagtcgtcg tcggcgcata
     5641 gtgcatggtg acaatgacga gatcggtatc gtggctggcg agggcatcga gcgcagcctc
     5701 cagccgggcg gcctcgcggc gcgcccgcac cgcaacggct tggctgaacc gggtcgagag
     5761 caaatcaggc gcctcgtcgg gccagaaccc gccgccgtag ccgccgatgc cgacgaggcc
     5821 gactcgcagt ccgtgccggc tttcaaccac ggtcgactcg ccgtccagca actcgacacc
     5881 ccccgtcgcc aatgcgcggc gaaactcctt gcggcgcagg gtgcgacggt cgtggttgcc
     5941 cataacggcg tagactggcg cgtcgacacc ggccatgacc cgtgcgacct gctccacctc
     6001 gatcttccgg ccgttgtccg tcatgtcgcc agtcagcagg accagatcga cttcgtcagc
     6061 ggccagccgc agcccctcct gcacggacat aggagcatcc gcccgaaggt gcacatcccc
     6121 gacggccatg acccggattc gttggacttc gcctgccgtc aaaccgacct cgatcgactg
     6181 gtgtcgtaga gtcattcgga cagttccggc ggtccggaga ctcctccgag tgtaccgcgt
     6241 gttcccggta ccctcgcccc ggcacggccg ctccacgtat actggagtac cgagccgcct
     6301 gccacatgca ctggccaacc tggccagcgg caacatgggc ggcgcacatc ctggccggga
     6361 gagagagttt gaccgtcacc accccaacag ggacaggcgc ctggcgaccg gcgcttgtgc
     6421 tcaccatcgt catcgccgtc ctgtctgccc tctgggtccc tactcagcca gccgctgccg
     6481 ccatacaggt gccagatgtc tccgcacgcg gcgtctacgc cttcgatctc gatacgggaa
     6541 tccagctctt cgccaagaac gagcacgagc gcatgccgat cggctcggtc gtcaaggtcg
     6601 tcaccgcgct ggtaaccgtc gagcataccg agctgagcga caaggtgatg atcgttgcgg
     6661 acgacctggt cgacgacccg ttatattcca acatgcagtt gcaggcggga gacacgctca
     6721 cggtgtcgca gctgctctac ggcctgatga tcccctcagg cagcgatgcc gcaaccgccc
     6781 tggcgcgcta cgtgggcgga attctctcga ggagcgacga tccaatcgcc cagctcgacg
     6841 cattcgttgc cgagatgaac gccttcgtcg accggcatgg actcgaaaac agtcgtttca
     6901 ccaacccgca cggactcgac gccccgaaca actactcctc cgcccacgac atctcgatcc
     6961 tgagcggcat gctgatgaag aacgagtttc tcgccaaggt ggtgcgagag ccgggctact
     7021 cattcatctc cgccggaccg gaggcgcgcc agtatcaggc ctcgacgacc aaccagctgc
     7081 taggagaggc cggcgtgatc ggcatcaaga ccggctcaac cgatgatgcc ggcggctgtg
     7141 tggtgctggc gcggcaggtc aacaatgcga attcgaccgt gatcaccgcc gtgctgggcg
     7201 ccgagttggc ctatgacgcg agtggcatga tcaccacgga cgagcgctgg gccgacgcgt
     7261 cccagctgct ccagttcatg gacacgtcct tcgcctggct gccgctcgcc gacgccgaga
     7321 ccttcgtcaa cctgcccacc gaactggctg tgtgggacgt tcagatcgcc gggaacccga
     7381 ccattccagt cgccgtgcgg gaggacacgc agacccgata ccagctcgcc ctcaaccccg
     7441 atggaggcgg gacgatcgat atctattacg atcaggttcg ggttggctcc ctgccgctgg
     7501 aggcggcaag cgcaacgtca ggtaatgtgg cgatagcggc atgagcgatc ggcgacgcga
     7561 cgacgagcat gagagcattc gggagtacca gggcgagcgt ttgcagcggg tgctctcaca
     7621 gcgcggcgtg gcgtctcgca gggcagcgga aaagatgatg ctcgacggtc gcgtgcaggt
     7681 gaacggtcga gtcgtcaccg agctcggcac gcgagtcagg ccgaaccggg acgagattcg
     7741 cgtcgatggc aaggtgctgc gaccacaacc ccctcgctac gtcatgctca acaagccgac
     7801 cggcttcatc accacggtca gcgacgagcg cgaccgctgg acggtgatgg acctggtcga
     7861 cgtgcgcgag cgggtctacc ccgtcggtcg gcttgaccgc aatacgcagg ggcttctgct
     7921 gctcacgaac gacggcgatc tggcccaccg ggtcatgcac ccgagctacc gggtcgacaa
     7981 ggaatatctg gcgttcaccg accagcggcc gacggagacc cagctgcagc gcctgcggca
     8041 gggcatcgac gtcgacggct acaaggtcgt gccggaagat gccaggctct ttcgggaaac
     8101 gtcagagggc gtcgccatcc gcattgtcat ccacgagggg ctctaccacg aagtacgacg
     8161 catgatggag gccgtcggca tcgagatcac gcggctgcgg cggacccgcc tcggcccctt
     8221 gcgactccag ggcattccat ccggcgcgtg gcgggatctc acgcccggcg agctggtcca
     8281 gctctaccag gctgtcgacc tagccgagga ggctgccgac cacatcaaca gtcgccgcac
     8341 gatcgtgcgt gagccagtcg ggggcttcac ccacaagggt gcaggtgcga gcggacagca
     8401 acgcggaccc ggactgcgcg gcgagcgcaa aggtgagccg acggagaccc gccagggcag
     8461 ccgatcagaa ggcagaatgg gccgaaatga caacgtacga tcatcgcaaa gtcatcgcaa
     8521 tcgacggccc cggcgccgcg ggtaagtcaa ccgtggcagc cgaggttgcc gatgagctcg
     8581 attccctgct gttcgatacg ggagcgctct accgcgtggt taccctgctg gcgctgcgca
     8641 ccggcacacc gatcgctgac ggcgaccggc tcgccgcgct cgcctccgag gcgacgatcg
     8701 agttatgccc tgcaaccgtg gaggacggtc gcaccagcga tgtgctgctc gacggcgatg
     8761 atgtgacctg ggagattcga cgaccggaga ttgatgccaa cgtctccgct gtctccgcac
     8821 atccccaggt ccgcggagcg ctgctcgacc tgcagcggga gatcgccgac ggaatcgccg
     8881 cagtgctggt cggccgggat atcggcacca tggtcatccc cgacgctgga ctcaagatct
     8941 ggctcgacgc gagcgtggag gaacgcgctc gccgccgcta tctggacatg caggagcgcg
     9001 gcatcgacat gtcctactcc gcaatcctcg aggaccttcg acggcgggac cagttcgatg
     9061 ccgaccgccc ggtctcaccg ctccgacgcg cccacgatgc gatcaccatc gtgacggatg
     9121 gacgcgatgt cgaagacatc gttcacgaga ttactgagct ggccagtgcg cgacaactcg
     9181 acaccggaac tgcccgatga gcgacttggc aggccacggg gcgcccgatc cggaagcggg
     9241 aacggtccgt gggatttggc gcaagctcat ccgaatccca ctctatgcct tcatttggct
     9301 cgtactccgc gtgcgcgtcg agggcatgga caacatcccg ccggaaggaa acggcgtgtt
     9361 tgttgtctcc aaccacttgc acaacgccga tccggtgctg ctcgagatcg tcttcccgcg
     9421 cccactgcat ttcatggcca aggaggagat ctaccgcatc aagccactcg gttggctggt
     9481 gagcaagttc ggcaacttcc cagtcgttcg aggcaagagt gaccgcaggg ccgtgcgcag
     9541 cgcgatcgct cggctgcagc acggcgtcgc agtgggcctt ttccccgagg gaacccgcag
     9601 caggacgatg cgcctcggcc ccgcgcgcgc aggggcaggg ttcattgcca atcaggccaa
     9661 cgtgccgatt ctgccggtcg cgattacggg gtccgagcgc ctgccgctca acgggaagcg
     9721 cccgccggcc gtgcgacctg tcgccgacca tcccgggata ttcatccgca tcggcgaacc
     9781 gttcacgctt ccaaaaaccg gaaacgacgg acgcaggctc agcccggagc aggcagcaga
     9841 tcgcatgatg tgggctattg ccgagcttct gccggaagat taccggggcg tctacccctc
     9901 gtcggagtcg tcgacggagc gggaggcttc cgcctccgcc tcgagtgccg cccgcacgaa
     9961 ctgatcggcg ctcagcacgt tgccctcctc gtcctgttcg ccccacggca tctcgaagtc
    10021 ggcgatatgg acggtatcgc cctcctgtac gccctgccgc tcgagttcgg cctggatgcc
    10081 actgccctca agcacccgtt ggaaccggtc gaccgaatcg ggcacgtcga agttggtcat
    10141 gcgcgtgaag cgctcgatgc caacgcccaa tacccgaaag tggtgcttcg agaggcgctc
    10201 cacgtcccac gcccgctcat cgacatcgcc gagcgtgtaa actcggcgct tcggttgctc
    10261 ctccgccgat cgcttctgct cggcttcccg ctccaggatc tcgcgcagct gggcggcgac
    10321 atcctgcacc aacggatcag tgccctcgcc ggtcgcggcc gaaatctcgt agatgcgctc
    10381 gtagccctgc tctataagcc aggaccggag acgatcgacg ttgtcgcgtg actcctggag
    10441 gtcgatcttg ttgagtgcga caaacatcgg cttttcaccc atctccggat cgaatgtctc
    10501 gatctcctcc atgatggtct ggaagtcctc gatcgggtag cgcccctcaa ggccgcccga
    10561 tgcgtccagc acgtggatca gcaccttcgt ccgctggacg tgccgaagga actcgtggcc
    10621 cagcccggcg ccaccagccg ccccttcgat caatcccggg atatcggcca tcacaaaggt
    10681 ctggccgccc gcacctccga tctcgaccac gccgaggttc ggttggagtg tagtaaacgg
    10741 gtagtccgcg accttcggct tagccttggt caccgtcgag aggagcgtgg acttgccagc
    10801 gtttggcagg ccgacgagcc cgacatcagc gatcaagcgc agctccagcc gtagccggcg
    10861 ctcctcgccc ggctcgccca gttcggcaac gcgcggtgcc cgccgggttg agctcttgaa
    10921 gtgaacgttg ccgagaccgc ccttgccccc ccgtgcgacg actagctcct cgccgggctc
    10981 ggtgaggtcg gcgatctgct cgccggcctc ctcaagaaag acgacggtgc caggtgggac
    11041 cttgacgatg gtgtccttgc ccttgcggcc atttctgttg cggcctgcgc cgtcaccgcc
    11101 attctccgcc ttgtacagat ggacgtactg aaacggcagc agcgtgttga gattcggcac
    11161 gacgcgaagc agaacgtcac cgccctttcc tccgtcgccg ccgtccggac cgcccttggg
    11221 aacatacttc tcacgacgga aggaagccga tccgttaccc ccattgcccg ccttcacatg
    11281 tattcgtgcg gtatcgacca gcattaaaca attctccttc gtaatcgcac gctactgtac
    11341 ctgagtcgca ggatgcctgc ctgcaacgcc gattgacgga tcttgagtgg gtcggagaaa
    11401 cacagggcgg ggctggtcgg tcccagcatt cgacggtagg cacggacagt gatgcgcacc
    11461 cggcgggcct tccagcagg
//
LOCUS       DASYWL010000062         4379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_546923, whole genome shotgun sequence.
ACCESSION   DASYWL010000062 DASYWL010000000
VERSION     DASYWL010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546923"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..741)
                     /locus_tag="VGR22_02140"
     CDS             complement(1..741)
                     /locus_tag="VGR22_02140"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEV2127399.1"
                     /translation="MTFTDRSTTRRDSSLTMTRRSAIRAVGGGAAALLTVATVGVAGA
                     NHTSQLYTVSANANFRIGPGTNYAIIAVITKGSTFTLNEQTQNGYAGIISNGRSGWVL
                     ASLVVAAGSTGTTPVISGSAWTADYVNLRSGPGTSYQVLRVVPKGSQIGTSTTVKNGF
                     RYVSHNGLAGWMADSLISATNPDDQGTNYKTATVNLNLRAEPSTSAKVLTVIPAGAKV
                     QLLHTQVGQYGNANYNGMQGWVAPAYLK"
     gene            1447..1797
                     /locus_tag="VGR22_02145"
     CDS             1447..1797
                     /locus_tag="VGR22_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127400.1"
                     /translation="MTADTIVSMLQASVVEAYGDADALLIGVDAETGAVVACPPLSQR
                     TWQVWVIAVQPGAVPLGVEVLGTLPLEDPRFQQAIGDTLGARICHPELLDSRELTVLE
                     GLRDRPRDDVRRTV"
     gene            1973..2728
                     /locus_tag="VGR22_02150"
     CDS             1973..2728
                     /locus_tag="VGR22_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IIL restriction-modification enzyme MmeI"
                     /protein_id="HEV2127401.1"
                     /translation="MHPVEFVEKWRGVTRTERSAAQEHFLDLCRVLDVPTPAEVDKHG
                     ATYTFEKGATKSTGGSGWADVWYRGHFAWEYKGPDKDLGKAYDQLLRYKDHLENPPLL
                     VVSDLNRIVIRTNFTNTTQETIEITLDTFAERESQDALRRMWTKPMSFRPGPGGRHRG
                     GRETLRRARRLARIARARPPRRRPLPRPAPLLPLRRGHRPAAEPNLQPPARLRQAEPR
                     RPARRDPAAAGNHGQRRPRRLRDHPSLQRRSLP"
     gene            3076..>4379
                     /locus_tag="VGR22_02155"
     CDS             3076..>4379
                     /locus_tag="VGR22_02155"
                     /inference="COORDINATES: protein motif:HMM:NF042814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEV2127402.1"
                     /translation="MTILDPACGSGNFLYVALNQLLNLEKEAITLGATEGGMPMAWPQ
                     VGPRQVMGLEINAYAQELAQVVIWIGYLQWMIGNGFGWREPVLEDLETIRLQDALLEE
                     RAGKVQRTVWPKADYIIIGNPTFLGDKAMRHELGQSYVAELRSVYMEVVPAGSDLCCS
                     FFEQARHAIDDELARRAGLLATNSIRGGANRRVLDRIKETGDIFMAWSDERWVVGGDA
                     AVRISIVGFDNGSEKERLLQGQPVEDINADLTGTVDMTRSERLAENRELAYFGVQKGG
                     PFDIPGSVAREWLHLPTNPNGRTNSDVVKPRLNGYDIVCRSQDNWIVDFGLDRTEAKA
                     AMYERPFEHVLHNVKPKRDQNPRKSYREKRWVHTEPRQGLRAKLEDLDRFICTPTVAK
                     HCVFIWADQSVVPDHQLVVFPREDDYFFGVLHSRAHEVWSLR"
BASE COUNT          777 a         1425 c         1420 g          757 t
ORIGIN      
        1 ctacttgaga taggccggcg cgacccaacc ctgcatgccg ttgtagttcg cgttcccgta
       61 ctgccccacc tgcgtgtgca gcagctgcac cttggcgccc gccgggatca ccgtcaggac
      121 cttcgccgag gtgctcggct cggcgcgcag gttcagattg accgtcgccg tcttgtagtt
      181 ggtaccctgg tcatcgggat tggtggcgct gatgaggctg tccgccatcc acccggccaa
      241 accattgtgc gagacgtagc ggaacccgtt cttcacggtg gtgctggtgc cgatctggct
      301 tccctttggc acaacgcgaa gcacttggta gctcgtgccg ggacccgacc gcaggttgac
      361 gtagtcggca gtccacgcgc tgcccgagat caccggggtg gtgccggtcg agccggccgc
      421 gacgacgagt gatgccagca cccagccact gcggccgttg gagatgatgc cggcgtagcc
      481 gttctgcgtc tgctcgttca gcgtgaaggt cgatcccttg gtgatcacgg cgatgattgc
      541 gtagttggtg cccggtccga tgcggaagtt ggcattggcg ctcaccgtgt agagctggga
      601 ggtgtggtta gcgccggcga cgccgaccgt ggcaacggtc aggagggcgg ccgcgccacc
      661 gccaacggcg cggatggcgg agcggcgggt catggtgagg gacgaatcgc gacgggtggt
      721 ggaacgatcg gtgaaggtca tggcggccct cgcgttctgc tgtgaatggg tccagcgtca
      781 gtgctggggg tgtggcccgt gccactgatc ccattctgca gatggcgccg accgcccaca
      841 tcggtaggct tgcctaatcg ccctaccgaa tgcctggcgc cgaccggccg gtcttggtga
      901 cctcagcccg cgtggtggaa ttcgccgctg ccggcgctgg agttcctagc ctccaggagg
      961 tcgacgacgc cggcatcgct tccatcaact gctgaagcag cgctgccggc gcctcatcgt
     1021 gctacgcccg ctcgtcgacg cgggctgccg accaccacca ccagctccac acctgcatcc
     1081 tcggcggcct ccgtcctgtc ccttgtcggc tccatgccgg cactctgttc caccccccaa
     1141 gctcgggata gcgagttcga tgtcacacat acggttacgg ctgcctgacc acccgagtcg
     1201 ggtccgtacc accacatcca caggcacgat cccggaggcc aagctggcct tgatagacca
     1261 cctgtactcc tgcataccag tggcagcgtg cggtcctggc acggacgggc gcggtgccct
     1321 gcccgtagcc ctgcccggcc acatacccga cttcatggca tgcaagttgt gatttggagc
     1381 ggcgcagtgg tcaacgcagc ggtcgccgac gaccgcccga agcgcattgg aaggaatgcg
     1441 atgatcatga ccgccgacac gatcgtttcg atgctgcagg cgtcggtcgt cgaggcatac
     1501 ggcgatgccg acgcgctcct catcggcgtg gacgcggaga ccggtgcggt ggtggcctgc
     1561 ccgccgctgt cgcagcgcac ctggcaggtg tgggtgatcg ccgttcagcc cggtgcggtt
     1621 ccgctgggcg tcgaggttct gggcaccctg ccgctcgagg acccgcgctt ccagcaagcc
     1681 atcggggaca cgctcggcgc ccggatctgc caccccgagc tgctcgacag ccgggagctc
     1741 accgtgctcg aaggattgcg ggaccgccct cgcgacgacg tgcgccgcac ggtctagcgc
     1801 ctcccaaccc caaagcacag gtatcgcggg ggccacccgt agcgtcggcc ttgtgccggc
     1861 gccctgttgc ccggacggca gccgtaggtt cccacgcatg gtttcgactc ggctccggta
     1921 tggatggaga cagcaactgc catcatcggg tcaccaccgg gagccgcacc ccatgcaccc
     1981 cgtcgaattc gtcgagaaat ggcgcggcgt cacgcgtacc gagcgctccg ccgcccagga
     2041 gcacttcctc gacctctgcc gcgtgctcga tgtgccgacg cccgccgagg tcgacaagca
     2101 cggcgccacc tacaccttcg agaagggcgc gaccaagtcg accggcggca gcggctgggc
     2161 cgacgtctgg taccgcgggc acttcgcgtg ggagtacaag ggcccggaca aggacctcgg
     2221 caaggcctac gaccagctcc tacgctacaa ggaccatctc gagaaccctc ccctgctcgt
     2281 cgtctccgac ctcaaccgca tcgtcatccg caccaacttc acgaacacga cgcaggagac
     2341 gatcgagatc acgctcgaca ccttcgccga gcgcgagagc caggacgccc tgcggcggat
     2401 gtggaccaag ccgatgtcgt tccggccggg gccgggaggg cgtcaccgag gaggccgcga
     2461 aacgcttcgg agagctcgcc ggctcgctcg gatcgcgcgg gcacggcccc cacgacgtcg
     2521 cccacttcct cgtccagctc ctcttctgcc tcttcgccga ggacaccggc ctgctgccga
     2581 accgaatctt cagccgcctg ctcgacttcg gcaagcagaa ccccgccgcc ctgcgcgacg
     2641 agacccggcg gctgctggaa accatgggca gcggcggcca cgtcgcctac gagaccatcc
     2701 ctcacttcaa cggcggtctc ttccgtgaag tgaaggcgct ggagctgacg ccggcggatg
     2761 tcgccaccct cgacagcgcc agccacctcg actggagcca ggtcgagccc gccatcttcg
     2821 ggaccctctt cgatcgctcg ctcggccccg cccagcgctc ccaactcggc gcgcactaca
     2881 ccggccgcga cgacatcctg cgggtggtcg agccggtcgt gatgcagcca ctgcgcggcc
     2941 ggtgggacga ggcccgcgcc gaactggagg ggctggtcga gaagcgggac gaggcgaaga
     3001 gcgctagcgc ccgcacccgg aaccagaacc gcatccagcg agacgctgcg ggccttccgc
     3061 gaggaactgg cgggggtgac gatcctcgac ccggcgtgcg gcagcggcaa ttttctctac
     3121 gtggcgctca accagctact gaatctggag aaggaggcga tcaccttggg cgcgacggag
     3181 ggcggcatgc cgatggcgtg gccgcaggtc gggccgcggc aagtgatggg gctggagatc
     3241 aacgcctacg cgcaggagct ggcgcaggtg gtgatctgga tcgggtatct gcagtggatg
     3301 atcggcaacg ggttcggctg gcgggagccg gtgctggagg acctggagac gatccgcttg
     3361 caggacgcgc tgctggagga gcgggccggc aaggtgcagc gaaccgtctg gcccaaggcg
     3421 gactacatca tcatcggcaa cccgacgttt cttggcgaca aggccatgcg tcacgagctt
     3481 ggccagtcat acgttgcgga actccgaagt gtttacatgg aggtggttcc ggctgggtca
     3541 gacctctgct gctccttctt cgagcaagcc cgacacgcta tcgacgacga gctagcgcgt
     3601 cgggccggcc tgctagcgac caactcgatt cggggtggag caaatcggag ggtactcgat
     3661 cgaatcaagg agaccggcga catcttcatg gcgtggtcgg acgagcgttg ggtggtcggt
     3721 ggcgatgctg ccgtacggat atcaatcgtt ggcttcgaca acggcagtga aaaagagcga
     3781 cttcttcaag gtcaacctgt tgaagatatc aatgctgact taacagggac cgtcgatatg
     3841 acccgttcag agcgactcgc cgagaatcgg gagcttgcct atttcggagt tcagaaagga
     3901 ggtccttttg atatcccggg ttccgttgcc cgagaatggc tgcatcttcc caccaatcca
     3961 aatggccgaa caaattccga cgtggtcaaa cctcgattga acggctacga cattgtctgt
     4021 cgcagtcagg acaactggat cgttgatttt gggctggata gaactgaagc caaggcggcg
     4081 atgtatgagc gaccgtttga acacgtactg cacaacgtga aaccgaagcg agaccagaat
     4141 ccacgaaaat cctacaggga aaaaaggtgg gttcacacgg agccgcgcca aggcttgcgc
     4201 gcgaaactag aggacttgga ccgattcatc tgcacaccca ccgtcgccaa gcattgcgtc
     4261 tttatctggg cggaccagtc tgtggtccca gatcaccaac ttgttgtgtt tcctcgcgag
     4321 gatgactact tcttcggcgt gctacacagc cgggcgcacg aggtctggtc gctgaggat
//
LOCUS       DASYWL010000063         3031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_528308, whole genome shotgun sequence.
ACCESSION   DASYWL010000063 DASYWL010000000
VERSION     DASYWL010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528308"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..699)
                     /gene="tsaE"
                     /locus_tag="VGR22_02160"
     CDS             complement(205..699)
                     /gene="tsaE"
                     /locus_tag="VGR22_02160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEV2127403.1"
                     /translation="MADSSTALTVRGAEAMRDLGRQIGQQLRAGDVLLLHGDLGAGKT
                     TLAQGIADGLGIESAVQSPTFTLMAEYAAAVGDRSPGTLYHLDLYRLDDPQELGEIGW
                     EEAMAPEQGVTVVEWPERAGDWLPDRFVLVSIAYQDAGNRKVTITSTPPDRAAELIAS
                     MEMG"
     gene            complement(692..1789)
                     /locus_tag="VGR22_02165"
     CDS             complement(692..1789)
                     /locus_tag="VGR22_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2127404.1"
                     /translation="MSPAADNPLPTAAPSALAVGGLTKAFDSYPIVTDVSFDVHQGEL
                     VALLGPSGCGKTTTLRIIAGLEHQDAGNVTVGEKVVADRSTFVPAERRKIGMVFQDYA
                     LFPHMSVGKNVAYGLRRGRESSERVDEVLELVGLLELKNRLPSELSGGQQQRVALARA
                     LAPGPDLVMLDEPFSNLDQKLRVSVRREVRAILVEAGTTAVLVTHDQEEALSLADRIA
                     VMNRGRIEQMGSPEELYHRPATRFVAGFIGDAQFLHAKARERTASTVLGDVPIVNESH
                     GEVDLLVRPEYIRLDRSRPEDGVVHGQVLSREYFGHDQLLMVRLDGGSTVTARLGAYS
                     GIRPGDGVWVSVRGAMVAFKTDHPLETGGDG"
     gene            complement(1858..>3031)
                     /locus_tag="VGR22_02170"
     CDS             complement(1858..>3031)
                     /locus_tag="VGR22_02170"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV2127405.1"
                     /translation="AWIVLTLFTYPYIYVSVRAALAGLDPSLEDASLSLGYGRWQTFF
                     RCVLPQLRPAVAAGALLAALYTISDFGVVTLLRYDVFTRAIYVQYRSSFDRSGAAVLG
                     LLLVLFAIGLVVAEAWVRGHSRYHSLGSGSRRRARKLPLGRWKVPALLFSGTVAALAL
                     GIPLFVLVYWIVRGVSGGEGLDRLPALVANSFLAGGLGAVVTTLAALPLAILAVRYRG
                     MIPQLAAGMSYLGYALPAIVIALAFVYFGANHVAWLYQTLPLLVIAYAVRFLPQAVGA
                     QRLSLLQINPRLEEASRSLGRSQAGTVARITVPLAQPGVLTGAALVMVTVMKDLPITL
                     ILAPIGFTTLATELWQFTSTGAYGRAAAPALALVLISMIPSLLLTGRHSDTDAVSS"
BASE COUNT          590 a         1022 c          953 g          466 t
ORIGIN      
        1 caccgaggat gccggcgacg tgttcccgga tgtcgcccag acccttgagg aagaggcgga
       61 gccggtcgtc cagcagctcg agtcggtcga gcccgagggg tagtctctcc agcgctcagc
      121 gcacggctac tcctgtaatg cgagcaaact gggggtggcg acaaaccatc cccggtttcg
      181 ccaccgcgcc acgccaggtc gccatcagcc catttccatc gacgctatca gttcagccgc
      241 gcgatccggc ggcgtgctgg tgatcgtcac cttccggtta ccggcatcct ggtacgcgat
      301 cgagacgagc acgaatcgat ccggcagcca atcgccagca cgctcgggcc actcgaccac
      361 ggtgacccct tgctccggcg ccatcgcctc ctcccaaccg atctcgccga gctcctgagg
      421 atcatcgagc cggtagagat cgaggtgata gagcgtcccc ggggagcggt cgccgactgc
      481 tgccgcgtac tccgccatga gcgtgaaggt cgggctctgg acggcgctct caattcccag
      541 gccgtcagca atgccctgcg ccagcgtcgt ctttccggcg ccgaggtcgc cgtgcaggag
      601 cagcacatcc cccgcgcgca gctgctgtcc gatctgccgg ccgaggtctc gcatcgcctc
      661 ggcacccctg accgtcagcg cggtgcttga atcagccatc cccgccggtc tccaagggat
      721 ggtcggtctt gaacgcgacc atcgctccgc gaaccgatac ccacacccca tcgccgggcc
      781 ggattccact gtacgcgccg agacgggcgg tcacggtgga gccaccatcg aggcgcacca
      841 tgagcagctg gtcatgcccg aagtactcgc ggctcaacac ctgcccatgc acgaccccgt
      901 cctccggtcg ggagcgatcg agtcggatgt actccggtcg gaccagcaga tcgacctcgc
      961 catgggactc attgacgatg ggcacgtcac cgagcacggt gctggcggtg cgctcccgtg
     1021 ccttggcatg gaggaactgc gcgtcgccga tgaagcccgc cacgaagcgc gttgccggcc
     1081 ggtgatagag ctcctccggg ctgcccatct gctcgatccg gccacgattc atgaccgcaa
     1141 tgcgatcggc aagcgagagg gcctcctcct gatcgtgggt gaccaggacc gccgtggttc
     1201 ccgcctcgac cagaatcgcc cgcacctcgc gacgcaccga cacccggagc ttctggtcca
     1261 gattggagaa gggctcatcc agcatgacca gatcgggccc cggcgccagt gcccgggcaa
     1321 gcgcgacacg ttgctgctgg ccgccggaca gctctgatgg caagcggttt ttcagctcca
     1381 gcaggccgac caactcgagc acttcgtcga cacgctcgct tgactcgcgg ccacgccgca
     1441 ggccataggc aacgttcttg ccgacactca tgtgcgggaa cagcgcgtaa tcctggaaga
     1501 ccatgccgat cttgcgccgc tcggctggaa cgaacgtgct ccggtcggcg acaaccttct
     1561 ccccgacggt cacattaccg gcatcctgat gctccaggcc ggcgatgatt cgcagcgtgg
     1621 tcgtcttgcc gcacccactc ggcccgagca gtgccaccaa ctccccttgg tgcacgtcga
     1681 acgacacatc ggtgacgatt ggataactat cgaatgcctt ggtcaatccg ccgacggcaa
     1741 gcgccgatgg cgcagcagtg gggagtgggt tgtcggcggc aggtgacatg gtactccgat
     1801 gtggtgcact cgtgcaggat cggactcgag atcccatatg gagtgtaccc cctgctgtca
     1861 ggagctcacg gcgtccgtgt cactgtgccg accggtgagc aggagcgaag ggatcatcga
     1921 gatcagcacg agcgcgagcg caggcgctgc ggcgcggcca taggcaccgg tgctggtgaa
     1981 ctgccagagt tccgtcgcca gggtggtaaa tccgataggc gccaggatca acgtgatcgg
     2041 caggtctttc atgaccgtca ccatcaccag cgcagcaccg gtgagcacgc ccggctgcgc
     2101 cagtggaacg gtgatccgtg cgacggttcc cgcctgggat ctgcccagtg accgtgacgc
     2161 ctcctccagc cgagggttga tctgcagcaa cgagagccgc tgtgctccga cagcctgtgg
     2221 caggaacctg accgcgtagg cgatcaccag caacgggagc gtctggtaga gccaggcgac
     2281 gtggttcgct ccgaaataga cgaacgccaa ggcaatcacg atcgccggga gcgcgtagcc
     2341 caggtaggac ataccagcag cgagctgagg aatcatcccg cggtaccgaa ccgccaggat
     2401 cgctaacggc agcgccgcca gcgtcgtcac aacggcacca agcccgccgg ccaggaagct
     2461 gttggcaacc agtgcaggca ggcgatcgag cccttcgcca cccgatactc cacgcacgat
     2521 ccagtagaca aggacaaaga gcggtatccc gagcgccagg gccgcgacgg ttccgctgaa
     2581 gagcagggcc ggaaccttcc agcgcccgag cgggagtttc cgcgcgcgtc gcctcgaacc
     2641 gctaccgagc gagtggtagc ggctgtgccc gcgcacccaa gcttccgcaa cgaccagccc
     2701 aattgcgaaa agaaccagaa gcaacccgag cactgcagcc ccactgcgat cgaagctgga
     2761 gcggtactgc acgtaaatgg cacgagtgaa gacatcgtag cgaaggagcg tgacgacgcc
     2821 gaagtcggag atggtgtaga gcgcggcaag cagtgcccct gccgcgacgg ccggccgcaa
     2881 ctgcggcaac acgcagcgaa agaacgtctg ccatcgcccg tatccgagcg acagagatgc
     2941 gtcttcgagc gaggggtcca ggcccgccaa ggcggcacga acgctcacgt agatatatgg
     3001 gtaggtaaag agggtcagaa cgatccaggc t
//
LOCUS       DASYWL010000064         8515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_603350, whole genome shotgun sequence.
ACCESSION   DASYWL010000064 DASYWL010000000
VERSION     DASYWL010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8515
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603350"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="VGR22_02175"
     CDS             complement(<1..997)
                     /locus_tag="VGR22_02175"
                     /inference="COORDINATES: protein motif:HMM:NF017691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HEV2127406.1"
                     /translation="MVRRRRHVTDMPVFRTMRSVTIAVVVVALWMAFATPLGAQTDEP
                     TVVLCNEATGEIVELPEWIAEGALHFPAHPATEAEIAAGECAVTAPEFGEGNGGNGVV
                     TLPVPTAVDPVSCGHFETQADAQAYFDSTELDDPSVLDPDGDGIACEFRFGEVNGGTE
                     ETPASFEPQTSALLINKHTCPSLEAAQAEVPRCELGDAGIRFEVMTTDGAEVGACVTE
                     VVTIQTVERAACWVENVPLGTVVVTEDVSTLPPGTAPLTESQTVTVRDPSTQGPDYIN
                     TAVFYNVPVVGEEPPTDGDPDGGGNTDGEVEGGDTGTGVVQLPDTGTGNVAPDARY"
     gene            complement(1252..3579)
                     /locus_tag="VGR22_02180"
     CDS             complement(1252..3579)
                     /locus_tag="VGR22_02180"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2127407.1"
                     /translation="MRRCDVRLLTLTGPGGVGKTRLALELGAELAREFADGARLVDLA
                     PIRDPALVPLGITQALGIRSDSGRPPPITLIEHLRDRSLLLVLDNLEQVVEAAPVVAQ
                     LLASCPGLKVLATSRIALNVRVEHEYPVAPLSLIDPVHRLNGATAATSPAVALFVERA
                     RAASPDFNLTDENASAVAQVCARADGLPLAIELAAARVKVLPPDTMLERLQSRLDLLS
                     GGPRDLPARLRTMRDTIAWSYGLLDRDAQALFRGASLFVGGFVFEAIEAVADDMEAIT
                     ILDNITALVSQNLLIQDTIDGQPRFRLLETIREYGIERLGAEGEVHAMRSRHAAWCLE
                     LAEEAEPELIREAQGRWLDRLEAEQDNMRAALDWLLAHREAEAGLCLASALWRYWATR
                     GDLREGYDWLERAVAQDAQVPAAMRGKALQRLGNLALDLGEYTAARAHYEASLTIRRP
                     QRDAAEIAEPLNGLGLLAFHQGDYAEAQCYHEESLQLRRSCGDAHGLGNSLVNLGDVA
                     AAQGDYERAETLYQEALAVRRRMGDTDGIAYCLFNLGDIAWGRGDQAAASARLEESLA
                     LFRRVGDRLGVGYALHSLGRVAHAEGDNRQAAALYGEALPLRQELGDKRGIIECVEGL
                     AAVASQARIERAVSLFAAADALRTAFNAPLRPADRSVQERELGAMRASLGAQPSDAAW
                     AAGLSLSLGQAITEAARIAKRVATDRETLPAGLSEREVEVLRLVAAGFTNAQIADTLY
                     LSPHTVSAHLRRIYSKLDVNSRSAATRYAADHGLV"
     gene            complement(4131..4565)
                     /locus_tag="VGR22_02185"
     CDS             complement(4131..4565)
                     /locus_tag="VGR22_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127408.1"
                     /translation="MADNRDRVNQDPLASTRGVQSEEVGDSFDAQMRDKTPERIREGA
                     ATNVTPGSQEKEVRELLPQLSDDEVSRLAVLEPGTQLPQGSIFLDLNDLQAGPFKAIG
                     GQEAGRDQRLIAKSETDYEIWNKLAGRDDEPEIERPEREQGS"
     gene            4746..6041
                     /locus_tag="VGR22_02190"
     CDS             4746..6041
                     /locus_tag="VGR22_02190"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2127409.1"
                     /translation="MTTRSRPLVAPPPSQRLHQREPAPPQPDTPEPEGAFVALRDPNF
                     ARYWSTLALSLTGTWVRITAMGYLVYEITGDPFKLAMLNVAMSGPQLIGNLIAGNILD
                     RVDRRRVLVGVQATYMAMMAILVALVVAGTVEYWHLIAISIVTGIAVGFDWPARLSLV
                     PSLVPKQRMQSAIALNASAFNASRILGPTLGGWLIATAGLAVAFGFTLTAAAPYALIL
                     LGLTIVRPQAQTARGNALAMMKVGYGYIWRHARIRSLMSVEIIPIALGMSYVTLAPAV
                     ASDILDVGSRGLGYMLGAVGVGSLIGSVLVAWLPGIRHRGRLVIVAVGLFGVLFIAYA
                     FSQSMLVTLPVMFLMGLTTALYSTIKETLIQTLVDDAYRGRVMATNSIFWSFTPLGAL
                     LAGALAAVFGLQTALAINGALVLLYAPLLWLATPVREID"
     gene            6305..7114
                     /locus_tag="VGR22_02195"
     CDS             6305..7114
                     /locus_tag="VGR22_02195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV2127410.1"
                     /translation="MADNPNGESGRSVRSLIKGVLNRSSAEAAQGHQTTPSDTTPREM
                     LRKERPQVDPVSIAASLGTPPDRPLRILGFGGTTSEQSWSLIPLEQALQVAESAGCET
                     TLATVYGLDLPLFRTDWRLEDYPDTLAWFIDEVRKADGLIICSPTYHGTISGAIKNIL
                     DMLIFLAWDDPPYLGGKPVGVMGYGGMTSMGVLDALTTCVRGLKGITVPTQVAVPESA
                     IYKPEAAIYSQRVLDRIDSMVADVISFSARLRVPHQKPDAISHASTSSPTG"
     gene            7286..7606
                     /locus_tag="VGR22_02200"
     CDS             7286..7606
                     /locus_tag="VGR22_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127411.1"
                     /translation="MANDARMKLLDLLDKKAFDPVLQASPDDYKGDDKQKLKDLQKTT
                     QSTKESYHEYGSAEKVREMFRDDLSSDAAQKVHKQLRDLGLPTLQDVKPEFEKLADEV
                     GVGH"
     gene            7699..8121
                     /locus_tag="VGR22_02205"
     CDS             7699..8121
                     /locus_tag="VGR22_02205"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2127412.1"
                     /translation="MTVPSPTGMHEIVLKVVDLAAAERFYGGLLGLAEVARWSGDRNA
                     VWFDAGDGVAIGLWTPETGGEKAIHHGRGGAHVHFALRIPHGSIDAWQERLEATGQPV
                     TRVDFDNGNRSVYIDDPADNCVELMDAVRDWSGNPIGG"
     gene            8199..>8515
                     /locus_tag="VGR22_02210"
     CDS             8199..>8515
                     /locus_tag="VGR22_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127413.1"
                     /translation="MTANPAFRLAAATQHDITIVDVAADGTATTTHTLTAPATSSVAI
                     DPLNPNRIVAGTFDRGPWLTIDGGRSWRHIGHGVHSTHISAVAMSAARHNHAGSGIYI
                     GTEP"
BASE COUNT         1419 a         3048 c         2584 g         1464 t
ORIGIN      
        1 ggtagcgtgc gtccggtgcg acattgcccg tgcccgtgtc ggggagctgc acgacgccgg
       61 ttcccgtgtc accaccttct acctcgccgt cggtattccc accgccgtca ggatcgccat
      121 cagtcggtgg ctcctccccg accaccggga cgttgtagaa gacggcggta ttgatgtagt
      181 ccggcccctg ggtgctggga tcacgaacgg tgacggtctg ggattcggtc agtggcgcgg
      241 tccccggcgg cagggttgag acgtcctcgg tgaccaccac cgtgccgagc ggaacgttct
      301 cgacccagca ggccgcccgt tcgacggtct gaatggtcac cacctcggtc acgcatgctc
      361 ccacctccgc tccgtcggtg gtcatcacct cgaaccggat gccggcgtca cccagctcgc
      421 agcgcggcac ctcggcctgc gcggcctcga ggctcgggca ggtgtgcttg ttgatcagca
      481 gcgcgctcgt ctgcggctcg aacgaggccg gggtttcctc tgtcccgccg ttcacctcgc
      541 cgaaccggaa ctcgcaggca atcccgtcgc catccgggtc cagcacgctg ggatcatcga
      601 gttcggtgga gtcaaagtaa gcttgcgcat cggcctgcgt ctcgaagtgc ccgcaactca
      661 ccgggtcgac cgccgtcggc acgggcaggg tcaccacccc gtttccgccg ttgccctctc
      721 cgaactcggg tgcggtgacc gcacactcgc ctgctgcgat ctcagcctcg gtggccgggt
      781 gggccgggaa gtgcagggcg ccctcggcga tccactcggg gagttcgact atctctccgg
      841 tcgcctcgtt gcagagcacg acggttggct catccgtctg agctccaagc ggcgtggcaa
      901 acgccatcca gagtgcgaca accacgaccg cgattgtcac acttcgcatc gtacggaaga
      961 ccggcatgtc cgtgacgtgc cgtcggcgac gtaccatgcc attcactcct ttacgcatat
     1021 acggccccgc ctctcgtgag gtgcggctcc tcaccgcacc ctcatcctac cgaggctcat
     1081 gctccaggcc atcacatgaa ttgactaatc actcgtggta atcgctgctg gcgcgcacgt
     1141 gcccagtggc gcgggcgatg cgatctcgga tcaccgcggc gtgtgtggca ggctttggct
     1201 cctggccatc tccggcgata cgccatgaca gcctatcgag cgaatcgaac ctcagacaag
     1261 accgtgatca gcggcatacc gggtggcggc actgcgcgaa ttgacatcca gcttgctgta
     1321 aatccgacga aggtgggcac tgactgtgtg agggctgagg tagagcgtgt cagcgatctg
     1381 cgcgttggta aaaccagcag ccaccagccg gagcacctcg acctcccgct cgctgagccc
     1441 ggccggcagc gtctccctgt ctgtcgcgac tcgcttggca atccgcgccg cttccgtgat
     1501 cgcctggcca agggacagcg ataacccggc tgcccaagcc gcgtcggaag gctgcgctcc
     1561 caaactcgct cgcatggcac ccaactcccg ctcttgcacg gaacggtccg cgggacggag
     1621 cggggcattg aacgctgtcc gcaaggcatc tgctgcggcg aagagggaca ccgcgcgctc
     1681 gatgcgtgct tgactggcaa ccgccgccag cccttccaca cattcgatga tgcctcgctt
     1741 gtctcccagc tcctgacgga gggggagcgc ctcgccgtag agggccgctg cctgccggtt
     1801 gtccccctcg gcgtgagcca cgcgaccgag gctgtgcagg gcgtagccga cacccagtcg
     1861 atcacccact cggcggaaca gggcaaggct ctcttcaagc ctggcgctgg ctgctgcctg
     1921 atctccccgc ccccaggcga tatcgccgag gttgaagagg cagtaggcaa tgccgtcggt
     1981 gtcgcccatc cgccgccgca cggccagcgc ttcctgatac agggtttcgg cccgctcgta
     2041 gtcgccttga gcagccgcaa catcgccgag gttcaccaag gagttgccca ggccatgggc
     2101 atctccacag gaccggcgca gttgcaggct ctcctcgtgg tagcactggg cttcagcgta
     2161 gtccccttga tggaaggcca gcagaccgag tccgttgagc ggctccgcga tttcggctgc
     2221 atcacgctga gggcgtcgta tggtgagact cgcctcgtaa tgagcacggg cggctgtgta
     2281 ctcgccgagg tcgagcgcca ggttgccgag ccgctgcagc gccttcccgc gcatcgccgc
     2341 aggaacctgg gcgtcctgag ccacggctcg ctccagccag tcgtatccct cccgcaggtc
     2401 gccacgcgtt gcccagtagc gccacagtgc ggatgccagg cagaggccgg cctccgcctc
     2461 tcgatgagcc agcaaccaat cgagcgctgc ccgcatgttg tcctgctcgg cttccagccg
     2521 atccagccac cgcccttgcg cttccctgat cagctccggc tcggcctcct cggcaagctc
     2581 caggcaccag gccgcgtgcc gggatcgcat cgcatggacc tccccctcgg cccccagtcg
     2641 ctcgataccg tactcccgga tcgtctcgag caatcggaaa cggggttgtc cgtcgatggt
     2701 gtcctgaatc agcaggttct ggctcaccag cgcggtgatg ttgtccagga tagtgatcgc
     2761 ttccatgtcg tctgctacgg cctcgatagc ttcaaacacg aagccgccga cgaagagtga
     2821 agcgcctcgg aacagtgcct gcgcatcccg atcgaggagt ccatagctcc aggcaatcgt
     2881 atcccgcatc gtccgaaggc gggccggcag gtcgcgaggg ccacccgaaa gcagatcgag
     2941 tcgggattgg agtcgctcca acatggtgtc gggtggaagc accttgacac gcgcagccgc
     3001 cagttcgata gccaggggca gaccgtctgc ccgcgcgcaa acctgtgcga cggccgacgc
     3061 gttctcgtcg gtcaggttga agtccggaga tgctgcacgt gcccgctcca cgaagagggc
     3121 aaccgcgggt gaagtcgctg ctgttgctcc attcaagcgg tgaacgggat caatcaggga
     3181 cagtggagcc acagggtact cgtgctccac gcggacattt agggcgatgc ggctcgttgc
     3241 caggaccttc agccctgggc aggatgcgag gagctgggct acgaccggtg ctgcttcgac
     3301 cacctgctca aggttgtcca gcaccagcag cagggaccgg tcgcgcaggt gctcaatgag
     3361 ggtaattggc ggtggtcgac cgctgtcact tcggattccc agcgcctgcg tgattcccaa
     3421 cggcacgaga gcagggtcgc gaatcggagc gaggtctacc agccgggcgc catccgcgaa
     3481 ctcccgcgca agctccgccc caagctcaag agccagccgg gtctttccga caccgccggg
     3541 tccagtcaac gtgaggagcc gtacgtcgca gcggcgcatc aactctcctg cctgggctac
     3601 gtcacgatca cgaccgatca agcgtgtggg aggcacaggc agctgggcag cttggggcga
     3661 ggcaggccga tcgttggacg catcctgcgc atcgagccgt acagtcaaga acactctcct
     3721 cagtagggct gcaggatcga tcgaggttgg agaacacgcc ggacgcgtcg catcagcaac
     3781 gctgttctct agtttcgaac gcccccactt gacttgaggt tacgccagga gcgccaatct
     3841 gtgcgtgggg ttcaggcccc gcgccacgct ccgcccagcc cggtatcctg ccggatgtcg
     3901 ccgatcgcac ttggcagcct cactcagtca tatggaacag tgtttctggg tgcccacgcc
     3961 agtggggctg gtacgtagca ccttcgaaaa agatgcgcga acgcgagcac gaggattacc
     4021 caggagaatg agctcctggg tgatccccgt tgggtggtcg ggtagaccac ctctcttgcc
     4081 cagtacagac aagaggcccg cggcgcccgt tcacatcgct ttttcgatac tcaggaaccc
     4141 tgctctctct ccgggcgctc gatctccggc tcatcatccc gcccggcgag cttgttccag
     4201 atctcgtagt ccgtctcgct cttggcgatc aggcgctggt ccctgccggc ctcctgcccg
     4261 ccgatcgcct tgaacggccc ggcctgcagg tcgttcagat cgagaaagat cgacccctgc
     4321 ggcaactgcg tccccggctc gaggaccgcc aggcggctca cctcgtcgtc cgagagctgc
     4381 ggcagcagct ccctcacctc cttctcctgg ctcccgggag tcacgttcgt tgccgccccc
     4441 tcccgaatgc gctccggcgt cttgtcgcgc atctgcgcgt cgaacgaatc gccgacctcc
     4501 tccgactgca cccctcgggt gctcgccaac ggatcctggt tcacgcgatc gcgattgtct
     4561 gccatgatgc tgtctccctg tctctcgtcg ttcggctgca ctcgccgcat ctcgacgcgc
     4621 aggttggatg ccagcaccct cgtccgcccg tttacgcccc ggcccggctc tgttagcgtg
     4681 gcacgagcag cagcccgccg cgcggcgcca ggcccgcagg ccccgtccaa agtgagcatc
     4741 acggcatgac gacccggtcc cgcccactgg tggcgccacc accgagccag cgcctgcacc
     4801 agcgggagcc ggcgccgcca cagcccgaca ctcccgagcc cgaaggtgcc ttcgtcgcgc
     4861 tgcgcgaccc gaacttcgcc cgctactgga gcacactcgc cctctcgctg accggcacct
     4921 gggtgcgcat cacggcgatg ggctacctcg tctacgagat caccggcgac cccttcaagc
     4981 tcgccatgct caacgtcgcc atgtccggcc cgcagcttat cggaaacctc atcgccggca
     5041 atatcctcga ccgtgtcgac cgccgccgcg tgctcgtcgg agtgcaggcg acctacatgg
     5101 cgatgatggc gatcctcgtc gcgctcgtcg tcgccggcac cgtggagtac tggcacctga
     5161 tcgccatctc gatcgtcacc ggcatcgccg tcgggttcga ctggccggca cgcctgtctc
     5221 tggtgccgtc gctcgtcccc aaacagcgca tgcagagcgc catcgccctc aacgcctcgg
     5281 ccttcaacgc ctcacgcatc ctcggcccga ccctcggcgg ctggctgata gccacggccg
     5341 ggctcgccgt cgccttcggc ttcaccctca ccgccgccgc gccctacgcg ctgatcctgc
     5401 tcggactcac catcgtgcgc ccgcaggcac agacagcccg cggcaacgcc ctcgccatga
     5461 tgaaggtggg gtacggttac atctggcggc acgcgcgcat tcgcagccta atgtcggtcg
     5521 agatcatccc catcgcgctc ggcatgagct acgtcaccct cgcgccagcc gtcgccagcg
     5581 atatcctcga tgtcggcagc cgcggcttgg ggtacatgct cggcgcggtc ggtgtcggct
     5641 cgctgatcgg cagcgtgctc gtcgcgtggc tccccggtat ccgccaccga ggccggctgg
     5701 tgatcgtcgc cgtcggcctg ttcggtgtgc tcttcatcgc ttacgccttc tcgcagtcga
     5761 tgctggtcac gctgccggtc atgttcctga tgggcttgac caccgcgctc tactcgacca
     5821 tcaaggaaac gctgatccag acgcttgtcg acgacgccta ccgcggccgg gtgatggcca
     5881 ccaactcgat cttctggagc ttcaccccgc tcggcgcgct gctcgcgggc gcgctggcgg
     5941 ctgtcttcgg gctgcagacg gcgctggcga tcaacggcgc actcgtgctg ctctacgcgc
     6001 cgctgctctg gctcgccact cccgtccgcg agatcgactg agatgtcctg gcgccgaccc
     6061 agccgtcatg ctgggcgcag cgaagcatcc ccgcgcgaag cgccggcacc gcaggtgccg
     6121 catccgatgc cgtgtcaact ggatgccgga tcaccgcccg gcgcttcccg attttccatc
     6181 cgcacacagc gaaatgcaac acggtgtatc gtgatatcgg ggaaacacgc cgaaacgcgc
     6241 ggcatgaacc gtgcgcattt tcggtatatg gatcgatcgc gcctcgcacg caggatgttc
     6301 aatcatggca gacaacccca acggtgagtc cggtcgctcc gtccggtcgc tgatcaaggg
     6361 ggtgctcaat cgctccagcg ccgaggcggc ccagggccac cagaccaccc cctcggacac
     6421 cactccacgc gagatgctcc gcaaggagcg cccgcaggtc gacccggtca gcatcgccgc
     6481 ctcgctcggc acgccgccgg accgccccct gcgcatcctc ggcttcggcg gcaccaccag
     6541 tgagcagtct tggagcctga tcccgctcga acaggctctc caggtggcag aatctgccgg
     6601 ctgtgagacc accctcgcta ccgtctacgg cctcgacctg cccctcttcc gcaccgactg
     6661 gcgcttggag gactaccccg ataccctcgc ctggttcatc gatgaggtcc gcaaggcgga
     6721 cggcctcatc atctgctccc ccacgtacca tggcaccatc tccggcgcaa tcaagaacat
     6781 cctcgacatg ctgatcttcc tcgcatggga cgacccgccc tatctcggtg gcaagccggt
     6841 cggtgtgatg ggctacggcg gcatgacatc gatgggcgtg ctggacgcgc tcacgacctg
     6901 cgtgcgcggc ctcaagggca ttaccgtgcc gacccaggtg gcggtgccgg agagcgcgat
     6961 ctacaagccc gaggcggcga tctacagcca gcgcgtgctc gatcgcatcg acagcatggt
     7021 cgccgacgtc atctccttct cggcgcggct gcgcgtgcca caccagaagc cggacgccat
     7081 cagccacgcc tccacatcct ccccgaccgg gtagccctgc gctggcatgc gggcaggagg
     7141 tcccctgtaa cgtggtcacg cgcatggcat gccaggtgcg cggagctcac ccgaccggga
     7201 tgcctagatc atggcatccc tggagagcga tcatcgccgt ggtgccgtgc agccgcacga
     7261 tgcccgagat caggaggaac ccacgatggc gaacgacgca cgcatgaagc tgctcgacct
     7321 gctcgacaag aaagccttcg acccggtgct ccaggcgtct cctgacgact acaagggtga
     7381 cgacaagcag aagctgaagg atctgcagaa gacgacgcag agcaccaagg agagctatca
     7441 cgagtatgga tccgccgaga aggtccgcga gatgttccgt gacgacctga gctcggacgc
     7501 cgcccagaag gtgcacaagc agctgcgcga cctcggcctg ccgaccctgc aggacgtcaa
     7561 gccggagttc gaaaagctcg ccgacgaggt cggcgtcggc cactgacgct tcaggagctc
     7621 accgtacatg cggggcgagg ctatccggca acacccgggt ggctcgccgg agatcaccta
     7681 cgggagtcga gcccatccat gaccgtcccc tcgccaaccg gcatgcatga gatcgtcctc
     7741 aaggtcgtcg acctcgccgc cgccgagcgc ttctacggcg gtctgctcgg cctcgccgag
     7801 gtcgcccgct ggtcgggcga tcgcaacgcc gtctggttcg atgccgggga cggcgtcgcg
     7861 atcggcctct ggacgcccga gaccggcggc gagaaggcca tccaccatgg gcgtggcggc
     7921 gcccacgtcc acttcgcgct gcgcatcccg cacggcagca tcgatgcctg gcaggaacgg
     7981 ctcgaagcga ctggccagcc ggtcacccgg gtcgatttcg acaacggcaa ccgctcggtc
     8041 tacatcgacg acccggccga caactgtgtc gagctgatgg atgctgtccg cgattggtca
     8101 ggcaatccca tcggcggttg atcgcttcag catccgagcc gtgtacactc cgtctgggtc
     8161 aggacgacac atcgcttcat cacgcggagg ctgctcccat gaccgccaac cccgccttcc
     8221 ggctcgccgc cgccacccag cacgatatca ccatcgtcga tgtcgcagcc gacggcacag
     8281 ccaccacgac ccacacgctc accgcgcccg ccacctccag cgtcgccatc gacccgctca
     8341 accccaaccg catcgtcgca ggaaccttcg accgcgggcc gtggctgacc atcgatgggg
     8401 gacgctcgtg gcgccacatc ggccatggcg tccactccac ccacatctcc gccgtcgcca
     8461 tgagtgccgc ccggcacaac cacgccggca gcggaatcta tatcggtacc gagcc
//
LOCUS       DASYWL010000065        15641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_190418, whole genome shotgun sequence.
ACCESSION   DASYWL010000065 DASYWL010000000
VERSION     DASYWL010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 15641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15641
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190418"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(343..2364)
                     /locus_tag="VGR22_02215"
     CDS             complement(343..2364)
                     /locus_tag="VGR22_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127414.1"
                     /translation="MLDIVAFTNAMRQGTVSFLRPLGLRPFEEASGEIVSFHDDDAVV
                     FPVARSDDRPEFALRIPLDDASNRQWPEQYADLAATVGAIQAHLPAEITILGVEDIPG
                     TEVALLYDWVPGETLTRRVTRSRIRRERDQLYDLLWPLADIAEAMRLGGMVHADIAPG
                     NIVVRPDGRMTLIDLDRMGRREAIDAITPRRRPGYRIPRGGGDPVEEDAFGLLVLMAS
                     CAILADAPVPIDHERAPESSHPMLLFSSWDLMDPRRSRLVREIEDELTPLSKRLLDLL
                     VDACTGPSDRIPALLREAVRITRRSVSPPDAIDAETVGTDWHVVPASPQETQTPTAVP
                     HRPAETAFAEEHGAVMSWPKPKPLDVAQEESAPVADTNEGAARARVPETLQWQTTTGL
                     VDMLQGLGERGVAIERPTRRGRRVEQRRQAVAAELQDALAHNDRDALVRLAMSGSLAE
                     LGESRRSDLLQVVRALSHEAISRAVASDDDGAVVTAIDDTVFGRDTDLDPVFRERVHL
                     ARSRLRWTDTMVEAIRTGDVDTCASLMENPPGGAVDRLPETVRNRARELVERHDAVRD
                     AHEFIAAGDAEGLVRALGRLVVVSPAWTDYLDADAVEHLLGIEQIEHHLVQRLRQGPV
                     PQANQWMVDMVIAAGRLPDADRKAGLTPGEVRSRIDLQSSARATTHPSD"
     gene            2514..4229
                     /locus_tag="VGR22_02220"
     CDS             2514..4229
                     /locus_tag="VGR22_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127415.1"
                     /translation="MPVQSRFTRTSSTEPQIQEEEIPVEEYGTGDGETPEAQLPEPQR
                     LSQEHAALAREVNDLATSLDEIDTRASGRLRDLAHAVNTPNERSRWNAVDLHQAFNIE
                     RISFEYATRRVGRQKVRLIEFADKLRNMLVLVPVFLTWFALAEAAQNYERFISANPDQ
                     AGQPFLLLWQQGFGEQANPLSPTFATVALIDAAVILVIIMLTFYAHGRREDAEDAVAN
                     VAQEFYARFDNILAEAGVMLARDRAAQPSQLARNVATLTDRFDQNSREILSLIDDERA
                     RLAEISEQRHEEFQDFALFARSMRAGAEQSNRMLAELRQLSRSLDSTVDRLSSDVAAT
                     GRHQSTLLSTLQNLEQLTSSAIQSDQAVTHRLAEAASAISESADTAATSADNANRLAE
                     AANQALRGIGELAQRVVDTSDELTRAMNRDTSSGEEVAASITRTTQQNQQVADTLAGL
                     ASDIEAIRSAFGDLANRSATQVRALNQLLDQQGSITQEITRATRELGSMGMSSAQRNR
                     EVREELTRLAQRLEQLTTSLNRAAQQVPTAENLQKAFAGALRSSIDTDRAAIEEERPA
                     RRWTR"
     gene            4269..5396
                     /locus_tag="VGR22_02225"
     CDS             4269..5396
                     /locus_tag="VGR22_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127416.1"
                     /translation="MNPFRPRRRNRRDMIVLGGWLFADLLLGLAMIFLVANTVGAPPP
                     TPTATPTPDELATAEASFAAVQAENEATREALQSDLAMMEQTAIAQENQVASERLAAT
                     QTAEARQIAATQTAEAEATEAAMSESELATREARATQEAVAAQATISAFATEQAESGQ
                     SQAILSAELATISAQATEVAGNLEAQATTQAEIAAVATEQAESGADVQATLAALEASN
                     QTSADALATAEAEQAALQERQSQIEATATAFVAVAAPGSLSPSSIEEVIQVDLSGVLS
                     GDEGAVQQAQETIRDVFEPYAEAEGCQAGFALTNSRAPELGQGVELSRAVNDLLVETV
                     PEVFGETVFEAIALPNTTPAGEVILQIFLYSGCEIIGGGEG"
     gene            5710..6036
                     /gene="trxA"
                     /locus_tag="VGR22_02230"
     CDS             5710..6036
                     /gene="trxA"
                     /locus_tag="VGR22_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV2127417.1"
                     /translation="MTKPVNATDATYQTEVSDADKAVLVDFWAPWCGPCRIVGPILEE
                     IASEEPEKVKVVKVNVDENQQHAAQLGVFNIPTMVVYKNGQPVDKIVGAMPKAQIMER
                     LRPHMS"
     gene            complement(6111..6599)
                     /gene="nrdR"
                     /locus_tag="VGR22_02235"
     CDS             complement(6111..6599)
                     /gene="nrdR"
                     /locus_tag="VGR22_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEV2127418.1"
                     /translation="MRCPFCHARDSRVVDSRELSGGDSIRRRRECVECHRRFTTYERV
                     EAVTITVVKKDGRREPFSQDKLRQKIRVALTKRPISEDTLDTIVSHVESEVMKKGTKE
                     VSSTEIGEAVLRELKATDQVAYIRFASVYREFADLEDLRREVEGLASPLDGRLIDGDH
                     GG"
     gene            complement(6647..7849)
                     /gene="ftsZ"
                     /locus_tag="VGR22_02240"
     CDS             complement(6647..7849)
                     /gene="ftsZ"
                     /locus_tag="VGR22_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEV2127419.1"
                     /translation="MDERAARQLARDDRSLVDEDARDMPRSRWSSSEDFVEAFARIKV
                     VGVGGAGGNAINRMVSSGVEGIEFIAVNTDAQALMSSRATAVVRIGDKLTKGLGAGGR
                     PEIGERAAEESIEALAEVMQGSDMVFIAAGMGGGTGTGASPTVARLAKDAGALTVAVV
                     TKPFGFEGGRRRRAAEEGIELLRETVDALITIPNERLLHMVDPKTTVSEAFQIADDVL
                     RQGIAGIADLITKPGVINLDFADVKTIMQDAGSALMAIGYGEGPDRCINAAREAIESP
                     LLEMNIQGAKGVLYNIAGGESLTLFETSEAAEVIRAAADDDAEIIYGTSIDESLGDAV
                     MITLIATGFDEVGEIDAFQFDRVSDRGPAAPTMRSGGGRPAAGGPPVTPEDDWDSESS
                     IIKFLRDR"
     gene            complement(8156..9280)
                     /locus_tag="VGR22_02245"
     CDS             complement(8156..9280)
                     /locus_tag="VGR22_02245"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HEV2127420.1"
                     /translation="MSNSQQGRRIRIAVLFGGQSDEHDVSIRSAQTVINAIDTDRYDA
                     VPIGITREGRWIVDGDPMAALVQASEELSLRSGGDVNGEAVGEVRAVASVAGAFALTS
                     GEREAVDVVFPVLHGPFGEDGSVQGLLEVAGVPYVGSGVLGSAVAMDKAMTKHVLTQV
                     GIPQLPWQLVTRRDIQDHTNLVVDRICESMGLPCFTKPANLGSSVGIARSSSREDLVA
                     ALHEAAYYDRRVVVEKAIDAREIELSVLGNEEPIASVAGEIVPRGGFYDYAAKYLDNS
                     TELIVPARLDPSLLGFLQEMAIDAFRALDLAGMARVDFFIERGTDQVYLNEVNTLPGF
                     TSISMYPMLWEASGIPISELINRLVGLALDRHSDRKRSAR"
     gene            complement(9273..11690)
                     /gene="murC"
                     /locus_tag="VGR22_02250"
     CDS             complement(9273..11690)
                     /gene="murC"
                     /locus_tag="VGR22_02250"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEV2127421.1"
                     /translation="MPTFQSSTITELPPPPARIHFVGVGGVSMSGLARILRDWGYVVS
                     GSDRQASPVTELLASLGIEVQIGHGDASLAGSADILVATPPALTGAPVEIEAAAANGV
                     EILRRGQLLGLIANARQPAAVAGSHGKSTTSGMLTTALIAIGAHPSYSVGATVASTGR
                     NADSGSGPHFVVEADEYDRALLWLKPEVAIITTVAFDHPDIFADQEDYDQVFVDFASE
                     VRPGGTLVISADDEGCRRVLARIGEDGADEQIITFGIGDDADWRYQRSGGGWQIVTPG
                     GDVFPITLRVPGAHNVANATAAVAAIVALGFPVKEAVRGVEAFAGVGRRFEHRGMVGG
                     VDVVDDYAHHPDEIRATLAAARERYPGRRMVALFQPHTFSRTALLLDEFAESLDEADV
                     PLLLEIYPAREVNTWGVTSASVAERMEHDTPVMATPDDAAGWLASHTHKGDVVLTLGA
                     GDVTVVGAKLIEALRLRATRTSGRRSVGKPAAGIPLEGTDAIIQRNGDMAMFTTIRLG
                     GSADFLIRAPTPDVTVAALVWAEREGLPVTVIGGGSNLLVSDHGVRGLVIVARTPGQR
                     AGSLVEVEDGGDSVLVRVGGQAPMSWLGRHAAEQGWAGLDWAVGLPGQVGGATINNAG
                     AHGTESKDHLVEIEVFEGGDLRRYDRDWLEPAPRMTRVKESPRPRPWTLLRSVFRLPK
                     ADPQTLVALAENHAAFRKRTQPSGACSGSIFVNPEGDFAGRLLEEAGIKGMQVGSMQF
                     SDKHANWMINTGGGTAWEAWQLIRTAQGIIRQRYGIDLRPEIECVGDPFDGDEDERHM
                     EQDQIDE"
     gene            complement(11699..13147)
                     /gene="murD"
                     /locus_tag="VGR22_02255"
     CDS             complement(11699..13147)
                     /gene="murD"
                     /locus_tag="VGR22_02255"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEV2127422.1"
                     /translation="MGRTNDLRNKRATLVGLGTLGGGVGVARYLVAQGARVTVTDMRD
                     RESLAGSIEALQGLPITWHLGGHDVRDFHPDGADLVVRNPGVPRDSAFLKTARKYGVP
                     IEMEMSLFFRACPAPVIGVTGTKGKTTVSTLIGEMLTVWDPTSVVAGNMGVSALAALD
                     RITPETPAVVELSSWQLEAMHEHRLGPKIAVLTNIHEDHLNHYNGFEDYASTKRTIGA
                     HVPEDGVVIYNADQPDTRRISEVTRACLFPFGLQPRGGEGAWLDDEALVVRWNGRENR
                     YGLPTHLALRGPAGIANALAAIAAATARGVPTGVIAGALRSFAGVPNRLELIASCGGT
                     RFINDTSATAPAAAAATIRLLAHEPGELTVIGGGADKSSDFSPLADAVAHTGVRLVLL
                     DGSGTARLQEALREHAVEPQTPFGSLADAFEAAAAGDTNERTVVLSPGCASFGMFRNE
                     FDRGDQFRELVRAWCAEHQPDHADVSDSSHAR"
     gene            complement(13158..14186)
                     /gene="mraY"
                     /locus_tag="VGR22_02260"
     CDS             complement(13158..14186)
                     /gene="mraY"
                     /locus_tag="VGR22_02260"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEV2127423.1"
                     /translation="MILLVPLAADAVMPSDPNENMAVSLALAALSFVVTVIVGRPLIT
                     ALRQRGVGKKIRLEGPEAHLSKTGTPTMGGLMISSTVVVITLVFNTVGRLSMLLPITV
                     LLAVSVLGAIDDRMNLVGGRSTGMTGRFKMLWLTIFALFAALVLYAPDPYGLGLHHMY
                     IPFLGRFDIGLIYIPVAMFAIIGSANAVNLTDGLDTLSAGTAAIAFLAYGIIAYRQGQ
                     LGVVTFCLTMVGALLGFLWFNAHPAQVFMGDTGSLSIGAALAVAAFMTGQWLLLPIVG
                     FVFLAEAGSVMLQVTYFKLTKGKRIFRMTPLHHHFELLGWSETQITMRFWLIGMLAGL
                     VGTALALL"
     gene            complement(14183..15607)
                     /gene="murF"
                     /locus_tag="VGR22_02265"
     CDS             complement(14183..15607)
                     /gene="murF"
                     /locus_tag="VGR22_02265"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEV2127424.1"
                     /translation="MMSYTLQEVLDGTGGTLRGDLDAGFRFRDIVRDSRTVAAGDLYW
                     AIQGERLAGHQFVGEAVRAAAAAAVVSRESLEEVPEAFPLIVVDNTIVALQRLAAWKR
                     NLMHADVIGITGSVGKTSAKESIAAVLAEGGKVYRSGGNYNNEIGLPLSILEAPLDVD
                     FLVLEMGGAYAFGEITHLSEIARPRVGVVTNVYPVHIERMGTIEAIAETKAELVDAIP
                     EDGVAILNGDDHRVRAMAERAKGRVVTYGLGEDVDVRASDVQSDGLKGTSFWLQIDGQ
                     RHHIRVPLVGAHGVQIALVALATGHAYGMHIAAMLVGLRNPGVQVRLVFERGPGGSQI
                     IDDTYNASTPSVLAALDVLRDVPGQRHVAVLGEMRELGDVSEEEHRVVGRRAGDLVDL
                     LFTYGELADPLAEAAQTVRREDRLPLEVRSFPIAEKAALIEALEGELHGGDVVLIKGS
                     RGLEMEEVVAALRRRSEDVQETGE"
BASE COUNT         2876 a         5406 c         4774 g         2585 t
ORIGIN      
        1 cgccgaagcg ctcggcctgg cgccgcgcgt gttccggcac caggtcggcg atgccgacga
       61 cccgcaggtt ggcgaagcgc gtcgtgaagt tctccaggta gatggcggag atgtagcccg
      121 ccccgatgat gccgacgccg atatctctgt tcctgttgtt cataagacac cttgtttggt
      181 cagtagccag cctatggccc agacaactaa cgggctctcg ctcctgtccg gatcaggact
      241 ctcaggagcg aaacccttcc acctcatcct ctacatgcat ctcaatgtgc cacgctcaca
      301 tcattggtcg ggccagccaa agccccactc acctgccaaa actcagtccg acggatgagt
      361 agttgcgcga gcggaactct gcaagtcgat ccgactcctg acctcgcctg gcgtcaggcc
      421 ggccttgcgg tccgcgtccg gtaaccgccc cgccgcgatc accatgtcca ccatccactg
      481 attcgcctgc ggaaccggcc cctgcctcag tcgctgcacg agatggtgct cgatctgctc
      541 gatgcccaac aaatgttcca ccgcatcagc gtcgaggtag tcggtccagg ccgggctgac
      601 caccaccagg cggccgaggg cgcgcacaag tccctcggcg tcccccgctg cgatgaactc
      661 gtgggcgtcc cgcactgcat cgtgacgctc gacgagctcc ctggcacggt tgcggaccgt
      721 ctccggcagc cggtcgaccg ctccgccggg cggattctcc atcagcgagg cgcacgtgtc
      781 cacgtcgccg gtccgaatcg cctcgaccat ggtgtctgtc caccgcaatc gggatcgtgc
      841 aaggtgaaca cgttccctga agaccggatc aagatctgta tcgcgtccga aaacggtgtc
      901 gtcgatagct gtgacgaccg cgccgtcatc gtcgctggcg accgcacggc tgatcgcctc
      961 gtgcgagaga gcgcgcacga cctgcaacag gtccgatcgc cgtgactcgc ccagctcggc
     1021 gagcgatccg ctcatcgcca gccgcactaa cgcatcgcga tcgttgtgcg cgagcgcatc
     1081 ctgcagctca gccgcaaccg cctgtctgcg ctgctccact cgtctgccgc gcctggttgg
     1141 tcgctcgatc gcaacgccac gctcaccgag gccctgcagc atgtcgacca gcccggtggt
     1201 tgtctgccac tggagcgtct ccggcactcg agccctcgcg gcgccttcgt tggtatcggc
     1261 gaccggcgcg gactcctcct gcgccacgtc cagcggtttt ggcttgggcc agctcatcac
     1321 cgccccgtgt tcctcggcga atgctgtctc ggccggccta tggggcacag ccgtcggtgt
     1381 ctgggtttcc tgcggactgg ccggaaccac atgccagtct gtcccaaccg tttcagcgtc
     1441 gatggcgtca ggcgggctga cggatcgacg ggttatccga acggcctcgc gcagcagcgc
     1501 cggaatacgg tcgctcggtc cggtgcacgc atcgaccagc aggtccaaca gtcgcttcga
     1561 cagcggcgtg agctcgtctt cgatttcccg cacgagccgc gagcgtcgtg ggtccatcag
     1621 atcccatgac gagaagagca acattgggtg ggacgactcc ggcgcgcgct cgtggtcgat
     1681 cggcaccggg gcgtcggcca gtatcgcgca cgaagccatc agcaccagca gcccgaaggc
     1741 atcctcctcg acagggtcac cgccgcctct cgggatgcgg taacctggcc ggcgacgcgg
     1801 tgtgatcgca tctatcgcct cccgccggcc catacggtcg aggtcaatca acgtcatgcg
     1861 gccgtcgggc cgcacgacaa tgttgccggg tgcgatgtcg gcgtggacca tgccaccgag
     1921 ccgcatggcc tcggcgatgt ccgcgagcgg ccagaggaga tcgtatagct ggtcgcgctc
     1981 acgtcggatg cgagatcgag tcacccggcg cgtcaacgtc tcgccaggca cccagtcgta
     2041 gagcagcgcc acctcggtac ccgggatgtc ttccacaccg aggatcgtga tctcggcggg
     2101 caggtgcgcc tgaatcgcgc caaccgtcgc cgcaagatcg gcgtactgct ctggccactg
     2161 ccggttgctc gcgtcgtcga gcggtatccg caacgcgaac tcgggccgat cgtcgctgcg
     2221 tgccacggga aagacgactg cgtcatcgtc gtgaaacgag acgatctcgc cggaggcttc
     2281 ctcgaagggt cgcagcccga gcggacgcag gaacgaaacc gtgccttggc gcatggcgtt
     2341 ggtgaatgca acgatgtcga gcacgcgtgc ctcctctacg gcgggcgcat atgtaacgtg
     2401 tgcttgccat ccccgccctg tgatggtggt aaagtgtaca tacactttgc acgacgccgc
     2461 catcccagcg gtgccaggtg tcctgcttca gttgtagaga aagggctcac cccgtgccag
     2521 tccaatcacg attcactcgc acgtcgagca ctgaaccgca gatccaggaa gaagagatac
     2581 cggtcgagga gtacggaacg ggtgacgggg agacccccga ggctcaactt ccggagccac
     2641 agcgcctctc gcaggagcac gcggctctcg cccgcgaggt caacgacctc gccacctccc
     2701 tcgatgagat tgatacgcgc gcgtcgggcc gcctgcgcga ccttgcccac gcggtcaaca
     2761 ccccgaacga gcgctcccga tggaacgcgg tcgatctcca ccaagcgttc aacatcgaac
     2821 ggatatcctt tgaatacgcc actcgacgtg ttgggcgcca gaaggtgcgg ctcatcgagt
     2881 tcgccgacaa gcttcggaac atgctggtgc tggtgccggt cttcctgacg tggttcgcgc
     2941 tcgccgaagc tgcgcagaac tacgagcgct tcatcagcgc caacccggac caggccggtc
     3001 agcccttcct gctgttgtgg cagcagggct ttggcgagca ggccaatcca ctctccccca
     3061 ccttcgccac cgtcgcgctc atcgacgccg ccgtcatcct cgtgatcatc atgctgacct
     3121 tctacgctca cgggcgtcgc gaggatgcgg aggacgccgt cgccaacgtt gcccaggagt
     3181 tctacgcgcg cttcgacaat atcctggccg aggccggcgt catgcttgcg cgcgaccgtg
     3241 ccgcgcagcc gtcgcagcta gcccgcaacg tcgccacgct gaccgaccgc ttcgaccaga
     3301 acagccgcga aattctctcg ctcatcgacg acgagcgcgc gcggctcgcc gagatctccg
     3361 agcagcgaca tgaggagttc caggacttcg ccctgtttgc ccggagcatg cgcgccggag
     3421 cggagcagtc caaccggatg ctcgccgagc tgcgacagct ctcacgctcg ctggacagca
     3481 ccgtcgaccg gctcagctcg gatgtcgcgg cgacgggccg tcaccagagc acgctgctct
     3541 ccacgctgca gaacctcgaa caactcacct cgtcggcgat ccagagcgac caggccgtca
     3601 cccatcgact cgcggaggcg gcctcggcga tctcggaatc ggccgacaca gccgcgacca
     3661 gcgcagacaa cgcgaaccga ctcgcggagg cggccaacca ggcgctccga ggcatcggcg
     3721 agctcgcgca gcgtgtcgtt gacaccagcg acgagctgac ccgagccatg aaccgagaca
     3781 catcctccgg cgaggaggtg gctgcatcca tcacgaggac aacgcagcag aaccagcagg
     3841 tcgctgatac gctcgccggc ctcgcaagcg acatcgaagc catccggtcg gcgttcggcg
     3901 atctcgcgaa ccgctcggcc acgcaggtgc gcgcgctcaa ccagttgctc gatcagcagg
     3961 gcagcatcac gcaggagatc acgcgcgcca cgcgagagct cggctccatg ggcatgtcca
     4021 gcgcacagcg caaccgcgag gtgcgggagg agctgaccag gctcgcgcag agactggagc
     4081 agttgacgac atcgctcaac cgcgcggccc agcaggtacc caccgccgag aacctgcaga
     4141 aggcctttgc cggagcactg cgatcgagca tcgacaccga tcgcgctgcg atcgaggagg
     4201 aacgccctgc tcgccgctgg actcgttagg ctctcccact cccacaacga tccttccagg
     4261 agacctgcat gaatccgttt cgaccacgca gacgaaatcg ccgcgacatg atcgtcctgg
     4321 gcgggtggct cttcgccgac ctcctgctcg gcctggcgat gatcttcctc gtggcaaaca
     4381 cggtcggcgc gccgccgcca acgccgacag ctaccccaac ccctgatgag ctcgccaccg
     4441 cagaggcctc gttcgccgcc gtgcaggccg agaacgaggc cacacgcgag gcactgcaat
     4501 ctgatctcgc catgatggaa cagacggcga tcgctcagga gaaccaggtc gcttcggagc
     4561 ggctcgcagc aacgcagacc gccgaagccc gacaaatcgc ggcgacccag actgccgagg
     4621 cagaggccac agaggcagcg atgtccgagt cggagctagc gacccgcgag gcacgggcca
     4681 cccaggaagc cgtcgcagca caagcgacca tctcggcgtt tgcgacggag caggcggagt
     4741 ccggccagag ccaggcgatt ctctcggcgg agcttgccac catctcggct caggcaacag
     4801 aggttgccgg taaccttgag gcgcaggcga cgacccaggc ggaaatcgcc gcggtggcga
     4861 ccgagcaggc tgagtccggc gccgatgtgc aggccacgct cgctgcgctg gaggcttcga
     4921 accagacgag cgccgacgcg ttggcgaccg cggaggccga gcaggcagcg ctgcaggaac
     4981 gccagtcgca aatcgaggca accgccacgg cgttcgtcgc cgtcgccgct cccggatcgc
     5041 tcagtcccag ctcgatcgag gaagtcattc aggtggatct gagcggcgtg ctctccgggg
     5101 acgagggtgc tgtccagcaa gcgcaggaaa cgatcaggga tgttttcgag ccctacgccg
     5161 aggcggaggg ctgccaggcg gggttcgccc tcaccaactc acgcgcgccg gaactcggcc
     5221 aaggcgtcga gctctcgcga gccgtgaacg atctgctcgt cgaaacagta ccggaggtct
     5281 tcggcgagac ggtcttcgag gcgatcgcgc tgccgaatac cacgccagcc ggcgaggtga
     5341 tcctgcagat attcctctac tccggctgcg agatcatcgg cggaggcgag ggctagcaga
     5401 cacgtgatct tgacatgtgc tcggggtggc agcgtgtgga cccggttgct attcggggca
     5461 tagttcgtgc gccaagttca atgagaagca cgctctccgc ggaggcgcgc ggacaagagt
     5521 tcgagtgttg aagtcttgcc gtggatgagc cgatcccgaa gagcaatagc gtctgaacgt
     5581 gcgcagagag ggcgctatcg acagaatctg tcattgccat gttcgagtga cgatcgagcc
     5641 gtgggagcgc ggttcctatg gtatcatttg ggaagtattg atccgattcc tgtacccatg
     5701 gaggtatccg tgaccaagcc agtgaatgcc accgacgcaa cctaccagac tgaagtcagc
     5761 gacgccgaca aggcggtgct ggtcgacttc tgggccccgt ggtgcggtcc gtgccgcatc
     5821 gtcggtccca tccttgagga aatcgcctcg gaggagcccg agaaggtcaa ggtcgtcaag
     5881 gtcaacgttg acgagaacca gcagcacgct gcccagctcg gtgttttcaa cattccgacc
     5941 atggtcgtct acaagaacgg ccagccggtc gacaagatcg tcggcgcgat gccgaaggcc
     6001 cagatcatgg agcgtctgcg cccgcacatg agctagcccg tcaccgcagc caagacaaag
     6061 gaaccgggga gccgagcggc tccccggttc ctttgtcttg gccgggataa tcagcctccg
     6121 tggtcaccgt cgatgaggcg gccgtccagc ggcgaggcaa gcccctcaac ctcgcgccgc
     6181 aggtcctcga gatcggcgaa ctcgcggtag accgaagcga agcgtatgta ggccacctgg
     6241 tccgtcgcct tgagctcccg cagcacggct tcaccgatct cggtcgatga gacctccttg
     6301 gtgcccttct tcatcacctc ggattccacg tggctcacga tggtgtcgag cgtgtcctcg
     6361 ctgatcggtc gcttggttaa cgccacgcga atcttctggc gcagcttgtc ctggctgaac
     6421 ggctcgcgtc gaccatcctt cttgaccact gtgatcgtta ccgcctcgac ccgctcatag
     6481 gtggtgaagc ggcggtgaca ctcgacacac tcacgacggc ggcggatcga gtcaccaccg
     6541 ctcagctcgc ggctgtcgac aacacgagag tctcgcgcgt ggcagaaggg gcatcgcacc
     6601 ggcggttacc gtctctttcc ggctcaggca aatccatagc gggaagctac cgatcgcgca
     6661 ggaacttgat gatcgacgat tcgctgtccc agtcgtcttc aggagtcacc ggcggcccac
     6721 cagcggccgg tcgaccaccg cccgagcgca tcgtcggtgc tgccggacca cggtcagaca
     6781 cccgatcgaa ctggaaggcg tcgatctccc cgacctcgtc gaaaccggtc gcgatcaggg
     6841 taatcatcac cgcgtcgccg agcgactcat cgatagaggt gccgtagatg atctcggcat
     6901 cgtcgtctgc cgccgcgcgg atcacctccg ccgcctcgct cgtctcaaag agcgtaagcg
     6961 actcaccacc ggcgatgttg tagagcacgc cctttgcgcc ctggatgttc atttccagca
     7021 gcgggctctc gatcgcctct cgggcggcgt tgatgcagcg gtccggcccc tcgccgtagc
     7081 cgattgccat cagcgcggag ccggcatcct gcatgatcgt cttgacatcg gcgaagtcga
     7141 gattgatcac gcccggcttg gtgatgagat cggcgatacc ggcgataccc tgacgcagga
     7201 catcgtccgc aatctggaac gcctcgctga ccgtcgtctt cggatccacc atgtgcagca
     7261 ggcgctcatt ggggatggtg atcagcgcgt cgaccgtctc acggagcagc tcgatgccct
     7321 cctcagccgc ccgccggcgc ctcccgccct cgaagccgaa cggcttggta accaccgcca
     7381 cggtaagggc tccagcatcc ttggcgagcc gcgcgactgt gggtgacgcc cccgtgccgg
     7441 tgccgccgcc catgccggca gcgatgaaga ccatgtcgct gccctgcatc acctcggcga
     7501 gcgcctcgat gctctcctcc gcggcgcgct cgccgatctc tggccgacct ccagcgccaa
     7561 ggcccttggt cagcttgtcg ccgatacgca ccacggcggt cgcccggcta ctcatcagcg
     7621 cctgcgcatc ggtattcacg gcgatgaact caatgccctc aacgccggag gaaaccatcc
     7681 ggttaatggc attcccaccg gcgcccccga caccgaccac cttgatccgt gcgaacgcct
     7741 caacaaaatc ctcagaggag ctccagcggg atcgcggcat gtcgcgtgcg tcttcgtcga
     7801 ccaacgatcg atcgtcgcgc gccaactgcc gagccgcgcg ctcgtccatc ggcgcgccac
     7861 ggccccgtcc ctgttgccct cgaccaggac cttgtccgcg ggattgcatc gtccctcacc
     7921 atccacacga gacaccgcca gcgccaccgc gccacactgt ctcattactc atgcgaaaga
     7981 gccgcagacc ataccggtca cgcggcgttg ctccggcgtt cccctgttca ccggccatcg
     8041 cgaccacaat cgaccatggt ggggtgtacg cacaatctga tagacgagta tagcggcaga
     8101 gccgatatca tcgccagttt gacatgcccc gaccgcacca cggcacgcta ccggctcacc
     8161 gagctgaccg cttgcggtca ctgtgccgat ccagcgccag gcccaccagc cggttgatca
     8221 gctccgaaat gggaatgccg ctcgcctccc acagcattgg atacatgctg atcgaggtga
     8281 agccgggcag tgtgttgact tcgttgaggt agacctggtc ggtgccacgc tcgatgaaga
     8341 agtcaacacg tgccattccg gcgagatcca gcgcccggaa ggcatcgatc gccatctcct
     8401 ggaggaaccc gagcagcgat gggtcgagcc tcgcgggaac gatcaattct gtcgagttgt
     8461 cgagatattt ggcagcgtaa tcgtagaacc ctcctcgtgg cacaatctcg ccggccaccg
     8521 acgcgatcgg ctcctcgtta ccgagcaccg acagctcgat ctcacgggca tcgatagcct
     8581 tctcaaccac caccctgcga tcgtagtacg ctgcttcgtg cagggcagcc acgaggtcct
     8641 cacggctgct cgatcgggcg atgccaacgc tcgaaccaag attggctggc ttggtgaagc
     8701 acggcagccc catggattcg cagatgcggt ccaccaccag attggtgtga tcctgaatgt
     8761 cgcggcgcgt aaccagctgc cacggcagct gcgggattcc gacctgggtg aggacgtgct
     8821 tagtcatcgc cttgtccatc gccacagcgg aaccgagcac tccggagccg acatacggca
     8881 ccccggcgac ctccagcaat ccctgcaccg agccgtcctc gccgaatggc ccgtgcagca
     8941 ctgggaagac aacatccacc gcctcgcgct cgccactcgt cagcgcgaac gcgccagcca
     9001 ccgacgccac ggcgcggacc tcgccgaccg cctccccgtt gacgtcgccg cccgaccgca
     9061 gcgagagctc ctccgaggct tgcaccagcg cggccatggg atcgccatcg acaatccatc
     9121 gcccctccct ggtgatgccg atcggcaccg cgtcatagcg atcggtatca atggcgttga
     9181 tgactgtctg cgccgagcgg atcgatacgt catgctcatc ggactgccca ccgaagagca
     9241 cggcgatgcg aatacggcgt ccttgctgcg aattactcat cgatttggtc ctgttccata
     9301 tgtcgctcgt cttcgtcacc atcgaagggg tcgccaacgc actcgatttc gggccgcaag
     9361 tcgatcccgt atcgctgccg gatgataccc tgcgctgtcc ggatcaactg ccaggcctcc
     9421 cacgcggtac cgccacccgt gttgatcatc cagttcgcgt gcttgtcgct gaactgcatg
     9481 gagcccacct gcataccctt gatccccgcc tcctctagca ggcgaccggc gaaatcaccc
     9541 tctggattga cgaagatgga gccggaacat gccccgctcg gctgggtgcg cttgcggaag
     9601 gcggcgtggt tttcggcgag cgcgaccagt gtctgcgggt cagccttcgg cagcctgaag
     9661 accgaccgca gcagggtcca ggggcgcggg cgagggctct ccttgactcg agtcatgcgc
     9721 ggcgccggct ccagccagtc tcgatcgtac cgccgcaaat cgcctccctc gaaaacctcg
     9781 atctcgacga gatggtcctt gctctcggtg ccgtgtgcac cggcgttatt gatcgttgcc
     9841 ccgcccacct gcccgggaag gccaaccgcc cagtcgagcc ctgcccatcc ttgctcggcg
     9901 gcgtggcgcc cgagccagct catcggtgcc tgccccccca cgcgaacgag cacgctgtcg
     9961 cctccgtcct cgacctcgac tagggatccc gcgcgctgac caggcgtccg ggcaacaatc
    10021 acgagcccgc gaacgccgtg atcgctgacg agtagattgc tgccgccccc gattacggtc
    10081 accggcagtc cttccctttc cgcccacacc agcgccgcca cagtcacgtc cggcgtcggc
    10141 gcgcgtatca ggaagtcggc ggatccgcca aggcggatgg tggtgaacat tgccatgtcg
    10201 ccgttgcgct gaataatcgc gtcggtcccc tcaagcggga taccagcagc aggtttgccg
    10261 acagagcgcc tgccagaagt gcgcgtcgcc ctcaaccgga gcgcctcgat cagcttcgcc
    10321 ccgacgaccg tcacgtcgcc cgcgccgagg gtcagcacca catcaccctt gtgggtgtgc
    10381 gaggcaagcc accctgctgc gtcatcggga gtagccatca ccggcgtgtc gtgctccatg
    10441 cgctcggcaa ccgaggcaga agtcacgccc caggtgttca cctcgcgtgc tgggtaaatc
    10501 tcgagcaaga gcggaacgtc ggcctcgtcg agcgactcgg cgaactcatc gagcagcagc
    10561 gccgttcgac tgaacgtgtg cggctggaag agcgcgacca tccgccgacc cggataccgc
    10621 tcacgcgcag ccgcgagcgt ggcacgaatt tcgtctgggt ggtgggcata gtcgtcgacc
    10681 acgtcgacgc cgcctaccat cccccggtgc tcgaagcgac gccccacccc ggcgaacgcc
    10741 tcgaccccac ggactgcctc cttgacgggg aaccccagag cgacaatagc ggcaacagcg
    10801 gcggtcgcgt tggcgacgtt gtgtgcaccc gggacgcgca aggtgatggg gaacacatca
    10861 ccacccggcg ttacgatctg ccagccacct ccgctgcgct ggtagcgcca atcggcatcg
    10921 tcgcctatcc cgaacgtgat gatctgctcg tcagccccat cctctccaat gcgtgccagc
    10981 acgcgtcggc acccctcatc gtcggcggag atgaccagcg ttccgccggg acgcacctct
    11041 gatgcgaagt cgacgaacac ttggtcgtag tcctcctggt ccgcgaagat gtcgggatgg
    11101 tcgaaggcca cggtggtgat gatggctacc tccggcttca gccagagcag cgcgcggtcg
    11161 tactcatctg cctcaaccac gaagtgcggg ccggagcccg agtcggcgtt acggccggtc
    11221 gaagcaaccg ttgctccaac cgaataggac gggtgtgcgc cgatcgcgat gagcgccgtg
    11281 gtcaacatgc cggaggtcgt cgacttgccg tgggatccgg ccacggcagc cggctgccgg
    11341 gcgttggcaa ttagaccgag caactgcccg cgtcgaagga tttcgacgcc attcgctgcc
    11401 gccgcttcga tctcgacagg cgcgccagtg agagccggtg gcgtggccac aaggatatcg
    11461 gccgacccgg ccaaggaggc gtcaccgtgc ccgatctgca cctcaatacc cagactcgcg
    11521 agaagctcgg tcacgggcga cgcctggcga tcggagccgg aaacgacgta gccccaatcg
    11581 cgcaggatgc gcgccagccc gctcatgctc acaccgccga cgccgacgaa gtggatacgg
    11641 gccggagggg gtggaagctc ggtgatggtc gatgactgga aggtcggcac agacaatgtc
    11701 acctggcgtg cgaggaatcc gacacatccg cgtggtcggg ctgatgctcg gcacaccagg
    11761 ctcgcacgag ctcacggaat tggtcgcccc ggtcgaattc attgcggaac atgccaaagc
    11821 tggcacaccc cggcgagagc acaaccgttc gctcgttcgt atcgcccgct gcggccgcct
    11881 cgaaagcgtc ggcaagggag ccaaacggtg tttgcggttc cacggcgtgc tcccgaagcg
    11941 cttcctgcaa cctcgccgtg ccgctcccat cgagcagtac caaccggacg ccggtatggg
    12001 caaccgcgtc ggcgagcggc gagaaatcgg aggacttgtc ggcaccgcca ccgatgacgg
    12061 tcagctcgcc tggctcgtgg gcaaggagcc ggatcgttgc ggccgcggcg gcgggcgcgg
    12121 tagccgaggt gtcgttgatg aatcttgtcc cgccacagct cgcgatcagc tcgagccgat
    12181 tgggcacccc cgcaaaggac cggagcgccc cagcgatgac gccggtcggc acgccacgcg
    12241 ctgtcgccgc cgcgatggcg gccagcgcgt tggcgatccc ggccggccct ctcaacgcga
    12301 gatgagtcgg cagaccgtac cgattctcac gcccgttcca acggacaaca agcgcctcgt
    12361 catcgagcca ggcaccctcg ccaccacgtg gctgcagtcc aaacgggaag aggcatgcac
    12421 gcgtcacctc actgatacgg cgggtgtccg gctgatcagc gttgtagatt accacgccat
    12481 cctccggcac atgcgcaccg atagtccgct tggtcgaggc gtagtcctcg aagccgttgt
    12541 agtgattgag gtggtcctcg tggatattcg tgaggaccgc gatcttcggg ccgagccggt
    12601 gctcatgcat cgcctcgagc tgccaactgg acagctcgac cacggccggc gtctcgggcg
    12661 tgatgcgatc gagtgccgcc agggcggaga cacccatgtt tccggccacc acgctggtag
    12721 gatcccagac cgtcaacatc tcgccaatca gcgtcgagac cgtcgtcttc cccttggtgc
    12781 cagtgacgcc gatgaccggc gccgggcagg cccggaaaaa gagcgacatc tccatctcga
    12841 tcggcacgcc atacttgcgc gccgtcttca ggaaggcaga gtcgcgcggg acgcccgggt
    12901 tgcgcacgac gagatcggcg ccatccggat ggaaatcgcg cacatcatgc cccccgagat
    12961 gccaggtaat gggcaacccc tgcagcgcct cgatcgagcc agcgagcgac tcgcggtccc
    13021 gcatatcggt gactgttacg cgggcgccct gagcgacgag gtagcgcgcc accccgacac
    13081 cgcccccgag ggtgcccaga ccgaccagcg tcgcccgctt gttgcgaaga tcattcgttc
    13141 ttcccatcgc ccctccccta gagcaacgcg agcgcggtgc ccacaagccc cgccagcatg
    13201 ccgatcagcc agaagcgcat cgtgatctgc gtctccgacc atccgagcag ctcgaaatga
    13261 tgatgcagtg gcgtcatgcg aaagatgcgc ttgcccttgg tcagcttgaa gtaggtcacc
    13321 tgcagcatca ccgagcctgc ctcggcgaga aaaacgaagc caacgatcgg caacaggagc
    13381 cactgaccgg tcatgaacgc cgccaccgcc agggccgcgc caatggagag cgagccagtg
    13441 tcgcccatga agacctgcgc cgggtgcgca ttgaaccaca ggaacccgag cagcgcaccg
    13501 accatcgtca gacagaaggt caccacgccg agctgtccct gcctgtaggc aatgatgccg
    13561 taggcgagaa acgcgatcgc cgccgtgcct gccgagagcg tatcgagccc atcagttaga
    13621 ttcaccgcgt tcgcggagcc aatgattgcg aacatcgcca ccgggatgta gatgagaccg
    13681 atatcgaacc gtcccagaaa cgggatgtac atgtggtgca ggcccaagcc atatgggtcg
    13741 ggtgcgtaga gcacgagcgc cgcgaagagg gcgaagatcg tcagccacag catcttgaag
    13801 cgtccggtca tgccggtcga cctgccgccg acgaggttca tgcggtcatc gatcgcaccc
    13861 aggacgctga ccgcgagcag caccgtgatc ggcaacagca ttgagaggcg accgacagtg
    13921 ttgaacacca gcgtgatcac cacgacggtc gaggagatca tgagcccgcc catcgtgggc
    13981 gtcccagtct tagagaggtg cgcctccggc ccttccagcc ggattttctt gccgacaccc
    14041 cgctgccgga gggccgtgat gagtggccgc ccgacaatca ccgtcaccac gaacgagagc
    14101 gcggccagcg ccagcgaaac cgccatattc tcgttcgggt cagacggcat cacggcatcg
    14161 gccgcgagcg ggacgagcaa gatcattcgc ccgtctcctg gacgtcctcg ctccggcgcc
    14221 gcagtgccgc aaccacctcc tccatctcaa gccctcgcga gcccttgatc aacacgacgt
    14281 caccgccgtg gagctcgcct tcgagcgcct cgatgagcgc cgccttttcg gcgataggga
    14341 aggagcgaac ctccagtggc agccggtctt cgcgacgcac agtctgtgcc gcctcggcga
    14401 gcgggtcggc gagttcacca taggtgaaga gtaggtcgac caggtcgccg gcgcgacgac
    14461 cgaccacgcg gtgctcttcc tcactcacgt cgccgagctc ccgcatctcg ccgagcaccg
    14521 ccacgtggcg ctgtccaggg acatcgcgca ggacgtccag cgcagcaagc accgatggcg
    14581 tactggcgtt gtaggtgtcg tcgataatct gcgagccccc cgggccacgc tcgaagacga
    14641 gccgaacctg taccccgggg ttgcggaggc cgaccagcat cgcggcgata tgcatgccgt
    14701 atgcgtgccc ggtggcgagc gccaccagcg caatctgcac cccgtgcgcg cccaccagcg
    14761 gcaccctgat gtggtggcgc tgcccgtcga tctgcagcca gaacgacgtg cccttgagcc
    14821 cgtcagactg cacgtcgctc gcgcggacat ccacatcctc gccgagcccg tatgtcacta
    14881 ccctgccctt cgcccgctcc gccatggcgc ggacgcgatg atcatctccg ttcaggatgg
    14941 cgacgccatc ttcgggaatg gcatcgacca gctccgcctt ggtctcggcg atcgcctcga
    15001 tcgtgcccat gcgctcgata tgaaccggat agacgttggt taccaccccg acacgtggac
    15061 gggcgatctc cgagaggtga gtgatctcgc cgaaggcgta ggctcccccc atctcgagca
    15121 ccaggaagtc gacatccagc ggagcctcga gtatggacag cggcaggccg atctcgttgt
    15181 tgtagttgcc gccgcttcga tagaccttgc ccccctcggc gagtactgcg gcgattgact
    15241 ctttcgcgct cgtcttgccg acgctcccgg tgataccgat cacgtcggca tgcatcaggt
    15301 tgcgcttcca tgcggccaag cgctgcagcg cgacgatcgt attgtcgacg acgatgagag
    15361 ggaatgcctc gggaacctcc tcaaggctct cccgcgagac aacggccgcg gcggcagcgg
    15421 cgcggaccgc ttctcccacg aactggtgac cggcgaggcg ttcgccttgg atggcccagt
    15481 aaagatcgcc ggcggcgacg gtccgcgagt cccgcacgat atcccgaaac cggaaccccg
    15541 cgtcgagatc gccgcgcagc gtcccgccgg tgccatccag cacctcctgc aacgtgtaac
    15601 tcatcatcga caacgcacgc tttcccgctc acacgttcgt a
//
LOCUS       DASYWL010000066         2519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_96590, whole genome shotgun sequence.
ACCESSION   DASYWL010000066 DASYWL010000000
VERSION     DASYWL010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96590"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VGR22_02270"
     CDS             complement(<1..332)
                     /locus_tag="VGR22_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127425.1"
                     /translation="MPRIAPINLPSMHQVMRSGFSGWRMLLIAWLAVFFSGPGQTYGT
                     SAFVDPMIEELGMSRSLFSTLYSIGTLASAAALMLLGRQIDRRGSRLVMALAALGLAG
                     GTVVLGVAG"
     gene            complement(339..647)
                     /locus_tag="VGR22_02275"
     CDS             complement(339..647)
                     /locus_tag="VGR22_02275"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEV2127426.1"
                     /translation="MESWSYIDALYFSVVTLTTVGYGDLTPSTDVGKLFTIFYILIGL
                     GVIAAFLSTFAASGIEILMKQRDAGARTDIRRRIRRRAATDMNQAEDEPAIDDRTGTT
                     "
     gene            complement(651..782)
                     /locus_tag="VGR22_02280"
     CDS             complement(651..782)
                     /locus_tag="VGR22_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127427.1"
                     /translation="MLILLLKLPRSVGTLVTAGFRNPATQGLTELALLLIAIGSVFY"
     gene            916..1698
                     /locus_tag="VGR22_02285"
     CDS             916..1698
                     /locus_tag="VGR22_02285"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEV2127428.1"
                     /translation="MCHKTCPKPVTGGAPIAEAMETVSRDDAEVPVLLAQPEGATGPM
                     SGIVLIHDIWGANDFYHDLARRLASEGYMVALPNLFHREGPLAEETREAALARSASAK
                     QDDHLADLDAVTRWLLEQPNSSGAFGVMGFCLGGTLTFLRAAREPIPAAGVAFYGFPA
                     RESTANAPIRPLDEPEVAAVAAPLLGLWGDQDSGVGMDNVKAYEEALDRHGKEHEFII
                     YPGASHAFLTFDPNAPTYETSRKAWDHALAFLAKHLRTSNIA"
     gene            1695..2321
                     /locus_tag="VGR22_02290"
     CDS             1695..2321
                     /locus_tag="VGR22_02290"
                     /inference="COORDINATES: protein motif:HMM:NF024423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV2127429.1"
                     /translation="MSTETDILTLLRHLLRLKATPRTGWIDRGVPETDAESIADHLLL
                     TTLLGWITAGDGLDHDRVLKLALVHDLVEAITGDPPPYDHEDLPLADDTEALRDFFSR
                     RHVRPAAAKAEKRLAEAEAMETLRALMPDSAAEEAAALWHEYEEQASPEARFVKDLDR
                     FEAFLQARDYARRFPELPFGGFTDMARKEITHPTLAALRDALLAEEER"
     gene            2318..>2519
                     /locus_tag="VGR22_02295"
     CDS             2318..>2519
                     /locus_tag="VGR22_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127430.1"
                     /translation="MICYRDVHGELHERPPDASVEWRVSGYGIIERDGRLLMVEPVMA
                     AGWTWGLPGGGVRLEPEETIIAG"
BASE COUNT          463 a          864 c          810 g          382 t
ORIGIN      
        1 cccgccacgc cgagcaccac agtaccaccg gcgagcccga gcgccgccaa cgccatcacc
       61 aagcgactgc cacgccggtc gatctgtcgt cccaacagca tcagcgctgc ggcgctcgcc
      121 agcgtgccga tcgaatagag cgtcgagaag agcgaccgcg acatccccaa ctcctcgatc
      181 atcggatcga cgaaagcgga tgtgccgtag gtctggccag gccccgagaa gaacacggca
      241 agccaggcga tgagcagcat gcgccagcct gagaaccccg accgcatcac ctgatgcatc
      301 gagggcagat tgatcggggc tattcgaggc atgagggctc aggtcgttcc cgtgcggtcg
      361 tcgatggcag gctcatcctc ggcctgattc atgtcagtgg cggcccgacg acgtatccga
      421 cgtcgaatgt ccgtgcgggc gcctgcgtct cgctgcttca tcaggatctc gatgccgctg
      481 gcggcgaacg tcgacagaaa ggctgcgatg acaccgaggc cgatcaggat gtagaagatc
      541 gtgaagagtt tgcccacgtc ggtgctgggc gtgaggtcac catatccgac cgtggtcagt
      601 gtgacaaccg aaaagtagag cgcatcgatg tagctccagc tctcgatctg tcagtagaag
      661 accgagccga ttgcgatcaa gagaagggcg agctccgtca gcccctgcgt ggccgggttg
      721 cgaaagccgg ctgtcaccag cgttccgacc gaacggggca gcttgagcag caggatcagc
      781 atgtgcaatc ggttctcccc ctctcgtccg catgccgctg gacctcgtcg acatttcgat
      841 tggcatgcgg caccattccc gaagaacgta gcccggcatg cgggtgcaca cggcgacgca
      901 cacaaggata ccggcatgtg ccacaagacc tgcccgaaac ccgtgaccgg cggcgcaccg
      961 atcgccgaag cgatggaaac tgtctctcga gacgacgccg aggtcccggt gctgctcgcg
     1021 caaccggagg gcgcgaccgg ccccatgtcc ggtatcgtgc tgatccatga tatctggggc
     1081 gccaacgact tctaccacga cctcgcccgg cgcctcgcca gcgagggcta catggtggcg
     1141 ctgccgaatc tcttccaccg ggagggaccg ctcgccgagg agacccggga ggcggcactc
     1201 gcccgcagcg cctcggcaaa gcaggatgac cacctcgccg atctcgacgc cgtcacgcgc
     1261 tggctgctcg agcaaccgaa ctcgtcaggt gccttcggag tgatgggctt ctgcctcggc
     1321 ggcacgctca ccttcctgcg cgccgcgcga gagccgattc cggccgccgg cgtcgccttc
     1381 tacggcttcc cggccaggga gagcaccgcg aacgcaccga tccgtcccct cgacgagccg
     1441 gaggtggccg ccgtcgccgc gccactgctc ggcctctggg gcgatcagga tagcggcgtc
     1501 ggcatggaca acgtcaaggc atatgaggag gcgctcgacc ggcacggcaa ggagcacgag
     1561 ttcatcatct atcccggcgc cagccacgcc ttcctgacgt tcgatccgaa cgctccaacc
     1621 tacgagacga gccgaaaggc ctgggaccac gctttggcct tcctcgcgaa acacctccgg
     1681 acatccaaca tagcgtgagc accgagaccg atatcctgac cctgctgcgg cacctgctcc
     1741 gtctgaaggc caccccgcgc acgggctgga tcgaccgcgg cgtgccggag acggatgccg
     1801 agtcgatcgc tgaccacctg ctcctcacca cgctgctcgg ctggatcacc gccggcgacg
     1861 gcctcgacca cgatcgggtg ctgaagctgg cgctggtgca cgacctcgtc gaggcgatca
     1921 ccggcgaccc gccgccctac gaccacgaag acctacctct cgctgacgac acggaggcgc
     1981 tgcgagactt cttcagccgt cggcatgtgc ggccggctgc tgcgaaagcg gagaagcggc
     2041 ttgccgaggc ggaggcgatg gagacgctgc gtgccctgat gccggattcg gctgccgagg
     2101 aggccgcggc actctggcat gagtacgagg agcaggcgtc accggaggcg cggttcgtca
     2161 aggatcttga ccgcttcgag gcctttctcc aggcccggga ctacgctcgc cgcttccccg
     2221 aactcccctt cggcggcttc accgacatgg cccgcaagga gatcacccac ccgacactcg
     2281 ccgccctgcg cgacgcgctc ctcgccgagg aggagcgatg atctgctatc gcgatgtcca
     2341 cggcgagctg cacgagcgcc cacccgatgc atccgtcgag tggcgggtca gcggatacgg
     2401 catcatcgaa cgtgacggcc ggctgctcat ggtcgagccg gtgatggcgg cgggctggac
     2461 gtggggcctg ccgggcggcg gcgtccggct ggagccggag gagacgatca tcgccggga
//
LOCUS       DASYWL010000067         7891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_584744, whole genome shotgun sequence.
ACCESSION   DASYWL010000067 DASYWL010000000
VERSION     DASYWL010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7891
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584744"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VGR22_02300"
     CDS             complement(<1..793)
                     /locus_tag="VGR22_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127431.1"
                     /translation="MPLLPMAGGAAAHPSGVVVVVWQPDGTAVAVDPHTGQPVPLPVS
                     TSNTSIEAPGACPEHDDSVVLVAGAVRYRQHWPKADAHLLLAGEGEGAGWWYWRRGLA
                     MHMLDLPVDLETAFPSNSCARWFHGALVHADHLGSGTLRLLAVDLATGEMMLDREFDR
                     RLELAATDVSPDGAAVAHLQGGNTRLDLWLADLRGQGRDVELGIPIGPAPVAPAAIDL
                     RVVTDGDRTVAIAGVSWSQPDHPAPFVLLVSVGNGEPVITTVPGEI"
     gene            complement(840..1514)
                     /locus_tag="VGR22_02305"
     CDS             complement(840..1514)
                     /locus_tag="VGR22_02305"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV2127432.1"
                     /translation="MGCAAVLAMAAGVTDVAAQGAFEPEGCWSEDERIEPDTEAENVF
                     LFPQWETAPDMVIDEDADYTAVIETNKGDMTFDLNIEAAPVAVNNFICLANNGFYDIV
                     PFHRVIEGFMVQSGDPTGTGAGGPGYRFEDELPGDDLDYTRGTLAMANAGPDTQGSQF
                     FIVHADLPADFPRDYTIFGELVEGEDVLDDIADSAVVPSLQGEPSRPVEFVVIEDITI
                     LEESGS"
     gene            complement(1667..2854)
                     /locus_tag="VGR22_02310"
     CDS             complement(1667..2854)
                     /locus_tag="VGR22_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEV2127433.1"
                     /translation="MATDIPHRSTEDLIGVDKALQIPLYAKRDIVLVRGEGPYLYDND
                     GKRYLDAMSNYGVAVLGHAHPRYTQALVEQAQKLTTTHQSFGNDARSEFLETLMIVAP
                     EGVRKAFLSNSGTEAIEAALKFARAATGREKIVAARRGYHGRTFGALSATADKKYRDP
                     FLPLPIPAEHVPFNDADALEAEIDDQTAAVILEPLQGEGGVHRAEPAFLHRAREVATA
                     KGALLIADEVQSAIRTGVPFVMVDAGVTPDIIATAKAIANGFPSGVTLVTDAVSEAMP
                     GGAHGSTFGGNPLASRAATETLRILKEDGLYANAREMGTRIIAGVEGMQSPKVRAVRG
                     SGMMIGIELKAKATPTLKALQERGVLALTAGNLVIRLLPPMIYGDEQVDELLAALSDV
                     LSA"
     gene            complement(3042..3413)
                     /locus_tag="VGR22_02315"
     CDS             complement(3042..3413)
                     /locus_tag="VGR22_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127434.1"
                     /translation="MGESDRGNFPTGPPAAGGRPVGRLRPRQPHVPQPDSLPGAGAGA
                     RFAGVWVDVAPEVAYARLLANRERPSRSNVPDASFRQITEEFEPPVNKPDVIRWQPGM
                     EIEELILELEMSLSQSCHPEP"
     gene            complement(3579..4232)
                     /locus_tag="VGR22_02320"
     CDS             complement(3579..4232)
                     /locus_tag="VGR22_02320"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV2127435.1"
                     /translation="MSERHIRAICFDVGETLIDETRFWSEVARYAGVPEFTLAGTIGG
                     LIERRESHRSIFGYLQIESVDTNVVGYRVERTDLYPDVDRTIDELQAQRYRIAIAGNQ
                     GEGVDEQIASLDLGAEVIGTSGLWGAAKPDPAFFQRVCDELNLQPGQIVLVGDRLDND
                     VLPAMEFGMQAVHIRRGPWGIIQAGWPESGQVRWKIDRLGQLCDTLECIEEAISDDA"
     gene            complement(4229..5050)
                     /locus_tag="VGR22_02325"
     CDS             complement(4229..5050)
                     /locus_tag="VGR22_02325"
                     /inference="COORDINATES: protein motif:HMM:NF010659.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[LysW]-aminoadipate kinase"
                     /protein_id="HEV2127436.1"
                     /translation="MLVIKIGGGAGIGADAFACFAADIATFTEPIVVVHGGNAEFSRL
                     SERLCIPPQIVTSSTGRVTRYTDAETMDAMLMAYCGKVNKRLVAAMHSAGVNAVGLSA
                     MDGGVAVGKRKPVLRGEIDGRAKVFRDDHAGTIEEIDSTLIRTLMDAGFVVLITPPAL
                     SHEGVPINVDGDKLAQKLAESLGADGLLFFSDTPGLLADRHDESTLIRAIDAGNPESA
                     LAAAEGRMVVKVEQAVKAVERGVGQVIFSDGRAERPITRALTGEGTVVREEPVTA"
     gene            complement(5161..5448)
                     /locus_tag="VGR22_02330"
     CDS             complement(5161..5448)
                     /locus_tag="VGR22_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEV2127437.1"
                     /translation="MREYFVYIAASPSRTIYIGVTNNLERRMYEHKRKLFKGFTAKYG
                     VDRLVHMEEFANIDDAIAREKELKGWRRARKVALIEEENPRWYDLSHSWFQ"
     gene            complement(5684..6730)
                     /gene="argC"
                     /locus_tag="VGR22_02335"
     CDS             complement(5684..6730)
                     /gene="argC"
                     /locus_tag="VGR22_02335"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEV2127438.1"
                     /translation="MIEVSVIGGSGYAGGELVRLLLFHPHVTLKQVTSERYAGKFVRM
                     VHPNLRKVTDMKFVKSADIEPCDVLFLALPHGVSMGKLAEVRDKAKAIIDLSSDFRLN
                     NPDDYPMWYSHEHTDLDALGRFVYGSPELHRDEIRTADWIASPGCMATTTILGLYPLF
                     QAGVVDLDMPVIADVKTGSSGSGGSPNLGSHHSERSGAMRSFKPTGHRHSAEIIQELT
                     VNDGVPKVSFSATAIEAVRGILATSHVFLKDDLQDKDLWKIYRTAYRDEPFMRIVKEN
                     AGVHRYPEPKLLSGSNWCDVGFEKDPHSTRVVVMAATDNLMKGAAGQAVQAFNIRMGC
                     DEATALEFPGLHPI"
     gene            complement(6941..>7891)
                     /gene="lysX"
                     /locus_tag="VGR22_02340"
     CDS             complement(6941..>7891)
                     /gene="lysX"
                     /locus_tag="VGR22_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysX"
                     /protein_id="HEV2127439.1"
                     /translation="HDSVDGLETEGPARMQELPIQSPLIVGVLMSRVRVEEKLLLAEL
                     SRRENVEVIRFDDRELVLDFHNPITSCDVVLERAINHLRAEYTLKVLNDWGVPTVNTF
                     EVANICGDKLLTTAALVKAGVPVPRTRIAFTPESALEAIEELGYPVVLKPAIGSWGRL
                     LSKVNDRDAAEALLEHKVTLGSFHHGAFFIQEYVEKQGRDIRSFVAGDETICAIYRDS
                     PHWITNTARGAKASNCPITSELDELSRAAARAVGGGVVAVDLFETPDGLQVNEVNYTM
                     EFRNSINTTGVDIPARIIDYTLAVAEQSRATRLATSAVAE"
BASE COUNT         1347 a         2579 c         2549 g         1416 t
ORIGIN      
        1 cgatctcgcc gggaacggta gtgatgacgg gctcgccatt gccgaccgaa acaagaagca
       61 cgaacggtgc gggatggtcc ggttgcgacc acgacacccc ggcaatggcc acggtacggt
      121 cgccgtccgt caccacgcgc aggtcgattg ccgccggggc gaccggcgcc ggaccgattg
      181 ggattcccag ctcgacatcc ctcccctgcc cgcgcaggtc cgccagccag agatccagcc
      241 gggtgttgcc gccttgcagg tgagcaaccg cagccccgtc tgggctgaca tcggtggcgg
      301 caagttctag acggcgatcg aattcgcgat caagcatcat ctcgccggtg gcaaggtcga
      361 cggcgagcag cctcagcgtc ccactgccga ggtgatcggc gtgaaccagc gctccatgga
      421 accagcgagc gcaggagttg ctcggaaagg ctgtctcaag atcgaccggc aggtcaagca
      481 tgtgcatcgc gagcccgcga cgccagtacc accagcctgc gccctcaccc tcgccggcga
      541 gcagcagatg ggcgtcggcc ttcggccagt gctggcggta gcgcaccgcg ccggccacga
      601 gcacgaccga atcgtcatgc tcggggcatg caccaggcgc ctcgatcgat gtattggacg
      661 tggagacggg cagggggacc ggctgaccgg tgtgcgggtc aacggcaacg gcggtgccgt
      721 ccggttgcca caccacaaca acgaccccgc tcgggtgagc ggcggccccg ccggccatcg
      781 gaagcagggg cacgccaagg attcctcgca gcaggtgtcg tcgcgtgatc atgtgcaaat
      841 caggagccgg attcctcaag gatcgtaatg tcctcgatca ccacgaactc aaccggtcgc
      901 gacggctcgc cctgcaggct cggcaccaca gcggaatcag cgatgtcgtc gagcacatcc
      961 tctccctcta ccagctcacc aaagatcgtg tagtcgcggg ggaagtcggc cggcaaatcg
     1021 gcatggacta tgaagaactg gctgccctgt gtgtccgggc cggcgttggc catcgccagc
     1081 gtgccgcgcg tgtaatcgag gtcgtcaccg gggagctcat cctcgaaacg ataccccggc
     1141 ccgccggcgc cggtgccggt cggatccccc gactgcacca tgaacccttc gatcacacgg
     1201 tgaaacggca cgatgtcgta aaagccgttg ttggcgagac agatgaaatt gttgaccgcg
     1261 acgggtgccg cctcgatgtt gagatcgaat gtcatgtcgc ccttgttggt ctcgatcacg
     1321 gccgtatagt cggcgtcctc atcgatcacc atgtcaggtg ccgtttccca ctgggggaag
     1381 aggaagacgt tctcggcctc ggtatcgggc tcgatgcgct catcctcgga ccagcacccc
     1441 tccggctcga acgcgccctg cgccgctaca tcggtgacgc ccgctgccat cgccaagact
     1501 gctgcgcagc ccacgatcgc tgtgttccat accctgttcc actgcctcac gttgataccc
     1561 tttctccgag acctgtgcag acagtctgaa ggagccgaca cgcggccggc cagaatccgg
     1621 gcggacacaa ggtccgcccc tacatatcgc gtactcggca aacgtatcac gcactcagga
     1681 catcgctgag ggccgcaagc agctcgtcga cctgctcgtc gccatagatc atcggcggca
     1741 gcagccgaat gacgaggttg ccagccgtca gcgcgagcac tccccgctcc tgcagcgcct
     1801 tgagggtcgg ggtggccttc gccttgagct cgataccgat catcatgccg ctgccgcgca
     1861 ccgctcggac cttcgggctc tgcatcccct cgacgccggc aatgatcctc gtgcccatct
     1921 cgcgcgcatt cgcgtagagc ccgtcctcct tgaggatgcg cagggtctcg gtggcggccc
     1981 tgctggcgag cgggttgccg ccgaaggtcg acccgtgggc gccacccggc attgcctcgc
     2041 tgaccgcgtc ggtgaccagc gtgacgccac tcgggaagcc gttggcgatc gccttggccg
     2101 tcgcgatgat gtccggggta acgccagcat cgaccatcac gaacgggacg ccggtgcgaa
     2161 tggcggactg cacctcgtcg gcaatcagca gcgcgccctt cgccgtcgcg acctcccgcg
     2221 cgcgatgcag gaaggctggc tcggcccggt ggacgccgcc ctcgccctgg agcggctcga
     2281 ggatcaccgc cgccgtctgg tcgtcgatct ccgcttccag cgcgtcggcg tcgttgaatg
     2341 gcacgtgctc ggccgggatc ggcagcggca ggaatgggtc gcgatacttc ttgtcagccg
     2401 tagcggagag cgcgccgaag gtgcgaccgt ggtagccgcg ccgggcggca acgatcttct
     2461 cgcggccggt ggcggcgcga gcgaatttga gcgccgcctc gatcgcttcg gtgccggagt
     2521 tgctcaggaa cgccttccgc acgccctccg gggcgacgat catcagcgtc tcgaggaact
     2581 ccgagcgggc atcgttgccg aagctctggt gggtggtggt gagcttctga gcctgctcga
     2641 ccagcgcctg cgtatatcgc gggtgggcgt ggccgaggac agcaacaccg taattgctca
     2701 tcgcgtcgag gtagcgcttg ccgtcgttgt catagaggta cgggccctca ccacgtacga
     2761 ggacgatgtc gcgcttggcg tagaggggga tctggagcgc cttgtcgacg ccgatgagat
     2821 cttcggtgga tcggtggggg atatcggttg ccatggtatt cctttggttg tgatgcgcac
     2881 tcgcgacgcg ttgaggatcc ttgcgaatca cgaaaatccc gcacaccgtc atgctgagca
     2941 cagcgaagca tccccgcgcg aagcgccagc agccccgctg ccgcgttcta ctcgaatgac
     3001 ggctggccgc agttctgccc ttcgggcagc ggcttcgccg ctcacggctc aggatgacag
     3061 gattgggaca acgacatctc caattctaga atcagctcct caatctccat ccccggctgc
     3121 cagcggatga cgtcgggctt gttgacgggc ggctcgaact cctcggtgat ctgccgaaag
     3181 gatgcatctg ggacattcga ccgcgacggc ctctcgcgat tcgccagcag gcgcgcatac
     3241 gcgacttccg gcgcaacatc gacccagacg ccggcgaacc gggcacccgc gccagcgccc
     3301 ggcagcgagt ccggctgcgg cacgtgtggt tggcgtggcc gaagacgacc gactggccgg
     3361 ccgccagcag cgggcgggcc agtcggaaag tttccgcgat cgctctcgcc catcccattc
     3421 cccgcgtgcc atcacacccg aggtcgatgt ttcccgtgat cgcgctcgca tcgacactga
     3481 gcagccgcat cctgccccgt tcggcgatct gccgggcgag cgtcgtcttt cccgcaaacg
     3541 ggatgccgct catggcgacg agcacaggtg accagagatc acgcatcgtc ggaaatcgcc
     3601 tcctcgatgc actccagcgt gtcgcacagc tgccctagcc ggtcgatctt ccagcgcacc
     3661 tgtccgctct cgggccaccc tgcctggatg atgccccacg gaccccgccg gatgtgcacg
     3721 gcctgcatgc cgaactccat cgcggggagc acgtcgttgt cgagccggtc tcccacgagc
     3781 acgatctggc cgggctggag gttcaattca tcacacaccc gctgaaagaa ggccgggtcc
     3841 ggcttggccg cgccccacag accggaggtg ccgatcacct cagcaccgag atcgagcgag
     3901 gcgatctgct cgtcgacccc ctcgccctga tttccggcga tggcgattcg gtagcgctgc
     3961 gcctggagtt catcgatcgt gcggtcaacg tcagggtaga gatcggtacg ctcgactcga
     4021 tagccgacga cattggtatc aaccgattcg atctggagat agccgaagat gctgcggtgg
     4081 ctctcccgtc gctcgatcaa tccgccgatc gtgccggcca gcgtgaactc gggcaccccg
     4141 gcgtaccgcg cgacctcgct ccagaagcgt gtctcgtcga tcagcgtctc accgacatcg
     4201 aagcagatcg cccggatgtg tcgctcgctc atgccgtcac cggctcctcc cggacgacag
     4261 tcccctcgcc tgtcagcgcg cgggtgattg gtcgctcggc gcggccgtcg ctgaagatga
     4321 cctgcccgac gccgcgctcg acggccttca cggcctgctc gaccttgacg accatccgtc
     4381 cctcggcagc ggcgagcgcc gactccgggt tgccggcatc gatggcacgg atgagggtgg
     4441 attcgtcatg gcggtctgcc agcaggcccg gcgtatcgga gaagaagagc aacccgtcgg
     4501 caccgaggct ctcggcgagc ttctgggcga gcttgtcgcc gtcgacgttg atcggcactc
     4561 cttcgtggga gagtgctggc ggggtgatga gcacgacgaa gccggcgtcc atcagcgtgc
     4621 ggatgagcgt cgagtcgatc tcctcgatgg tacccgcgtg gtcgtcacgg aagactttcg
     4681 cccggccgtc gatctcgccc cgcaggaccg gcttgcgctt gccgacggcg acgccgccgt
     4741 ccatggcact gaggccgacg gcgttgacgc ccgcgctgtg catggcggcg acgaggcgct
     4801 tgttgacctt gccgcagtag gccatcagca tggcgtccat ggtctcggcg tcggtgtacc
     4861 gggtaacgcg gccggttgac gaggtgacga tctgcggcgg gatgcagagc cgttcggaga
     4921 gacgactgaa ctcggcattg ccgccgtgga caacgacgat cggctcggtg aaggtcgcga
     4981 tgtcggcggc gaagcaggcg aaggcgtcgg caccgatccc cgcgccccca ccgatcttga
     5041 ttaccagcat tccaagactc ccaaatcact cagtttatgc atggtcaatc cagcgaaact
     5101 gatgtcatcc tgagcgaaga aggatcccct ggcgcgaagc gccggcctcc atcgcgttct
     5161 tcattggaac cagctatgac tcaggtcata ccacctcgga ttttcttcct cgatcaacgc
     5221 cactttgcga gctcgacgcc atcccttgag ctccttctcg cgggcgatag catcatcgat
     5281 gttggcgaac tcctccatgt ggaccagccg gtcgaccccg tacttcgcgg tgaacccctt
     5341 gaacaacttg cgcttgtgct cgtacatgcg gcgttccaga ttgttggtca caccgatgta
     5401 tatcgtgcgc gaaggactag cggcaatgta gacgaagtac tcgcgcacag cgtgctactc
     5461 cgcgtgagaa agggccgggc cttcggcccg ggggactctt cgctgcgctc agaatgaccc
     5521 cctctaggct ggccctgagt tcctcaattg tcgcccattt cgggcggacc ggcacaaggc
     5581 cggcgctacg cgaaagatgc caaaaggacc tgcccggctt gacacactcg ctgctggcaa
     5641 accgggggcg gacatggagt ccgcccgtgc gcatcgagcg ggcctagatc gggtggagac
     5701 cggggaactc gagggcagtg gcctcgtcgc agcccatgcg gatgttgaag gcttggacgg
     5761 cctgtcccgc cgcgcccttc atcaggttgt cggtcgcggc catgacgacc acacgcgtcg
     5821 agtgtgggtc tttctcgaag ccgacgtcac accagttgga accggagagc agcttcggtt
     5881 ccggatagcg atgcacgccg gcgttctcct tcacgatccg catgaagggc tcgtccctgt
     5941 aggccgtgcg gtagatcttc cagaggtcct tgtcctgcag gtcatccttg aggaagacgt
     6001 gcgaggtcgc caggatgccg cgcacggcct cgatcgccgt ggcggagaag gacaccttcg
     6061 gcacaccatc gtttacggtg agctcttgga tgatctcggc ggagtggcgg tggccggtcg
     6121 gcttgaacga ccgcatcgcg ccgctgcgct cagagtggtg cgagccgagg ttgggcgacc
     6181 cgccggaacc ggacgagccg gttttcacat cggcgatgac cggcatgtcg aggtcgacga
     6241 cgcccgcctg gaagagcggg taaagcccga ggatggttgt ggtcgccatg cagccgggac
     6301 tggcgatcca gtcggccgtg cggatctcgt cgcgatgcag ctctggtgag ccgtagacga
     6361 acctgccgag cgcatccaga tcggtgtgct cgtggctgta ccacatcggg tagtcgtccg
     6421 ggttgttgag ccggaaatcc gagctgagat cgatgatggc cttcgccttg tcgcgcacct
     6481 cggcgagctt gcccatggag acgccatgcg gcagcgcgag gaagagcacg tcgcagggct
     6541 cgatgtccgc gctcttgacg aacttcatgt ccgtcacctt gcgcaggttg ggatgcacca
     6601 tacggacgaa cttgccggcg tagcgctctg aagtgacctg cttgagcgtc acgtgcgggt
     6661 ggaacaggag aagccgcacg agctcgcccc cggcgtatcc cgaacctccg atgactgata
     6721 cctcgatcat gttgtgtttc cctgtgtcat atgccgggag catgcccggt gtcgcgtcaa
     6781 aaaccaaccc aaactgtcat cctgagctga cgccgcaggc gtcgaaggat ctcttgcgtg
     6841 gtggtgacca gattcgagtc ctggctgtcc cggcgtttga gatgcttcga cacacgggct
     6901 gtcgcccgcg gctcagcatg acaaccacga ctgtagatac ctattccgca acagcagacg
     6961 tcgccagcct cgttgcacgg ctctgctcgg cgacggccag cgtgtagtcg atgatcctcg
     7021 cggggatatc aaccccggtc gtgttgatgc tgttgcggaa ctccatcgtg tagttgacct
     7081 cgttgacctg caggccgtcc ggcgtctcaa agaggtcgac ggcgaccaca cccccgccaa
     7141 cggccctcgc cgcggcgcgg gagagctcgt cgagctccga ggtgatgggg cagttgctcg
     7201 ccttcgcgcc ccgggcggtg ttggtgatcc agtggggcga gtcgcggtag atggcgcaga
     7261 tcgtctcgtc gccggcgacg aacgagcgga tgtcgcgccc ctgcttttcg acgtactcct
     7321 gtatgaagaa cgcaccgtga tggaacgacc cgagcgtcac cttgtgctcc aggagcgctt
     7381 cggcggcgtc gcggtcgttg accttggaga gcagccgccc ccacgagccg atcgcgggct
     7441 tcagcaccac cggatagccg agctcctcga tcgcctccag cgccgactcg ggtgtgaagg
     7501 cgatgcgggt gcgcgggacc ggcacaccgg ccttgaccag cgcggctgtg gtgagcagct
     7561 tgtcgccgca gatgttggcg acctcgaagg tgttgacggt cgggactccc cagtcgttga
     7621 gcaccttgag cgtgtactcg gcacgcaggt ggttgatcgc gcgctccagc acaacgtcgc
     7681 agctcgtgat cgggttgtgg aaatcgagca cgagctcacg gtcgtcgaag cggatgacct
     7741 cgacgttctc gcggcgggag agctcggcga gcaggagttt ctcctcgacc cgcacgcgcg
     7801 acatgagcac gccgacgatg agcggggatt gaatgggcag ttcctgcatg cgggctggtc
     7861 cttccgtttc gaggccgtca acgctgtcat g
//
LOCUS       DASYWL010000068        15621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_21303, whole genome shotgun sequence.
ACCESSION   DASYWL010000068 DASYWL010000000
VERSION     DASYWL010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 15621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15621
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21303"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VGR22_02345"
     CDS             complement(<1..421)
                     /locus_tag="VGR22_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127440.1"
                     /translation="MRSEARRPIRPLVVVCLVLMLLTGGVSTLAAQGVIDPNADSDLD
                     GTKNSMDPDDDNDGVSDDQDYYPLDPTRSQQETAATPTPVTVSPTPTPGPALPPTPTL
                     APVTIRSPTSTGTATRISSIPTTITTGSWTSRMPTRST"
     gene            674..1063
                     /locus_tag="VGR22_02350"
     CDS             674..1063
                     /locus_tag="VGR22_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127441.1"
                     /translation="MTRTPLTVIPDPGLTRSTLLREGEDSIIFSGDFEGPVFTCGGCD
                     APLIEGVRPTYVVDLVLFCRACGAFSESPASSLADIDLAKIERPVMFPPGRYELSDAI
                     TVHDRWVMASERALQGTRIAAPVRYET"
     gene            1237..1953
                     /locus_tag="VGR22_02355"
     CDS             1237..1953
                     /locus_tag="VGR22_02355"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEV2127442.1"
                     /translation="MTTSDSTTPSRAAPAVDSGQRGVIWDMDGTLIDSARYHYESWRE
                     LLAPEGVDLDYPAFLVGFGRRNDDWLRELLGAEIALAEIRRIGDAKEERYREMLREEG
                     LVVLPGVLDTLRALREEGWQHAVATSAPVQNLEIILEVTGLKPLMDGWAGSEDVEQGK
                     PAPDVFLVAANHIGVAPQRAVVIEDAAAGVLGAHRAGMSVIGVGPHHAELGADRSAES
                     LLDLPIETFAELVMMASSAR"
     gene            2019..2092
                     /locus_tag="VGR22_02360"
     tRNA            2019..2092
                     /locus_tag="VGR22_02360"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2051..2053,aa:Cys,seq:gca)
     gene            2185..2775
                     /locus_tag="VGR22_02365"
     CDS             2185..2775
                     /locus_tag="VGR22_02365"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV2127443.1"
                     /translation="MPPIDISTDTTRPHHEERIGSRRAYDGHILHVRVDDVRMPTGRE
                     TVREMVEHPGSVIVLPVTTDDAVVFVRQYRYTVGEALIELPAGLIDEGETPEVAAARE
                     LAEETGYEAGELHFLGAAYVSPGYSQEESRFYVATGCSRGKHEPNEEEPMDVLHVRLA
                     DLPEMVKPGETIIRNAQTLLAVNWFLRVQNKIMGTP"
     gene            complement(3152..4285)
                     /locus_tag="VGR22_02370"
     CDS             complement(3152..4285)
                     /locus_tag="VGR22_02370"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2127444.1"
                     /translation="MASPDTVRLGIIGTGLAVEKLHWPALKRMTDRIRVTTFADIDRD
                     HAEHFVSYSGASMDGFTADYHELLGRDDVDAVLISLPIPLNYTVTKDSLEAGKHVICE
                     KPTGGSAEAMRAFIDLEGQYRDRTILIAENWFYRDDLRFARSLLDEGAIGRMHLMAWR
                     NVSQLIPREGEFSSTPWRWEGDYVGGPQLDAGVHNTAQIRLLCGDVDHVSGEIQDANS
                     THNGPSDLTMNLHFVSEAIGSYTASYPELAMPEEPNDMRLYGTEGVMSISRGGVRIHR
                     PDGSTECWKVEMPDAGYYNEFLNFHQALTGEAELVGTIAQSVRNMEIIVQGLASAEEG
                     KVMEMASGPIDLSPTAVPLWKPAGADGLFDGLGVTTDHEVTKG"
     gene            4485..5138
                     /locus_tag="VGR22_02375"
     CDS             4485..5138
                     /locus_tag="VGR22_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127445.1"
                     /translation="MVMMLNALLAVLFLFVQTPQAGLPSDPSALTELAAEYCATEVAQ
                     ATYTNVEIVEVEDVGLPPVVTYAAFDFETAEQAIAALEEAPLLVVQAYTEDPAVELED
                     YDDFAAEAPTPDYGDADIAHVITLPVLEDEDDVLVIELLGIVQESQLLLVLMFSDSVA
                     YPAAPGVALETLLPFGEELDETWDGTGDLQDAIPEEDEMPLNWVRDDVTVEAPPNCE"
     gene            5206..5853
                     /locus_tag="VGR22_02380"
     CDS             5206..5853
                     /locus_tag="VGR22_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127446.1"
                     /translation="MILHMLIAVLLSLTQVSQTVESDDPNVFIDLGSEHCATGIVFAA
                     YEYVVEDEVAETANPAYMTYSAFQFESVDQAVAAFEDAPVLVAETFSDDPDISDDPAF
                     DELVTEVVTADYGDISNAYIMTLPLEGEEPDILTIEMLSIVEEAQLLLILMFSETGTT
                     RPSVGLTLETVPPFIGDLDERWEGIGDLEEAVPAEDEMPLGWEGERVILEDPPDC"
     gene            6110..6682
                     /locus_tag="VGR22_02385"
     CDS             6110..6682
                     /locus_tag="VGR22_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127447.1"
                     /translation="MRHPLGAGVKTAVFVLKVLPTLPSRPVDWVTPPPVRERVRYPSL
                     HGEVEAEIARPASRGPHPGIVVCLGVVPFGVDHPQVPRLQGALTRAGFATLLYWSPNM
                     RDWRLDPVDVEDLAMAYEWLVAQPFVDPERSGLFGTCVGGSFALMASADARIRDRLAF
                     VGAFSPYASMRTLTRDIATGTWLREGGREV"
     gene            6713..7180
                     /locus_tag="VGR22_02390"
     CDS             6713..7180
                     /locus_tag="VGR22_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127448.1"
                     /translation="MQSLTAGLDPAEAARLREAMAEPAGQVDPAALSPAARTVYPLLT
                     RLTIDEVESATDALPQALRDRLDALSPMAYLPNLRSPIIVLMHAHDDSVIPRTESLQL
                     RDALADRPGLRYTEFIMFKHLDPSKVQMRILPLLRELGRFYLALYPMFRGGTG"
     gene            complement(7192..7644)
                     /locus_tag="VGR22_02395"
     CDS             complement(7192..7644)
                     /locus_tag="VGR22_02395"
                     /inference="COORDINATES: protein motif:HMM:NF042610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6789 family protein"
                     /protein_id="HEV2127449.1"
                     /translation="MNRYLIGAVAGVIATAIMTIAIEIGKLVGLLRMPPPEQVAVNVT
                     RRVGMTSRSPEPEFTAGTLVTHHGYGVVVGMLYVAVRRFLPGSSGLAGLVWGAVVWTT
                     AYLGYLPALRLYPWPDDDRPSRTVVMIVAHAVYGTTLVETEKRLRVRM"
     gene            7813..9045
                     /locus_tag="VGR22_02400"
     CDS             7813..9045
                     /locus_tag="VGR22_02400"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2127450.1"
                     /translation="MTASSDAPTTPSFGWRQLLLNREHLAFALPGFIGRLPLAMRALG
                     CVLLIQGITGEYGLAGLVGAAQTLVSAFASPRIGQLADRHGTRPVLSIALVIQTIGVI
                     ALITTAYASAPAPVLLLAAAVIGGSSVPFGSFSRARWTQHVARGRDLDRAYALEGVFE
                     EISFIVGPALVVFLSIEIAPPLGLIVALLMTFVASAGLIRLPEIAHHAMQAPDAPRTS
                     LLRNPVILVVAGSGIGMGILFGAIEISLVAFAEELGREATASALIAIFTVGSLVAAVL
                     YGVLTISHPLRWRLLASVVWVGLWMIPTALAESLPAMIVCVLFAGIGISPWAISGSSM
                     IERGVPAHTLREGFGWFSAATASGAALGATLAGMAIDRWESDGGQVVSILGGMLAIAV
                     ALLGQRRLRELAPGSMPL"
     gene            complement(9032..9625)
                     /locus_tag="VGR22_02405"
     CDS             complement(9032..9625)
                     /locus_tag="VGR22_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEV2127451.1"
                     /translation="MTNRVLVSMIVGLVAVLVVSGGIRLATAQDEETDTAPYCATEAE
                     AELLRRINDLREERGLDPLQLSQPLGRAAELKARDMAQDNYLAHISPEGQGPEELLDQ
                     VGYTYNTAIGENIAAGQRSAEGTFEQWLNSPEHREIMLGEQFTAVGIGRAHNPEAKYD
                     WYWAAEFGGKVGEPADVCESATPQQGAGERVRPFTRA"
     gene            10495..11448
                     /locus_tag="VGR22_02410"
     CDS             10495..11448
                     /locus_tag="VGR22_02410"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEV2127452.1"
                     /translation="MARFAETMPYAFFEGEIVPFGEANVSIGTHALQYGTGAFAGVRG
                     YLDQDGKTINIFRLREHAARLLRSARLLRMDLPYDADAVGQILIDLAQKNAPSSDVYF
                     RPFVYKSSVQLTPRLQGIDDQLAVYMMLMGDYLDTNRGQKAIVSSWTRISDNAIPSRG
                     KITGAYINSSLAKDEAEEKGADEAIMLTADGTVAEGSGCNLFIVRDGVLVTPPITSDI
                     LEGITRRSLLKMAQDLGIPIEERGIDRSELYIVDEAFFAGTGVQIARIDTIDGRQIGT
                     RGEAPISKRLSKVFFDTVRGANDLYRDWITPVQIPVEAACR"
     gene            11663..12595
                     /locus_tag="VGR22_02415"
     CDS             11663..12595
                     /locus_tag="VGR22_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127453.1"
                     /translation="MVSFIRLVLGLLVAVASATALPLATTAAQQPSCADYSTAAAAQF
                     ALDINPVLASALDPDRNGAACDHEDASGTTDGAGGLQLPETQAPAQMPASNAPAEVTV
                     DAIDQQPADTTTMATQPSTTQQAGVLDGRLGGSQAAFAAAHGQPIEEVPSDTNPAVVL
                     HAYPPPSTAVDLYTIDLNDRVAIVVVAAETSWGSQQAADLIADYLPPDVTELGDVEPL
                     ADGSLLIPIFSEDLATGVSAATMTQAVISGVPGDLYLLLFPDDANGVVEMEIGLGNGD
                     DLREDVQTSGPVPEPTATQQATGPGLAPTQTPSN"
     gene            12635..12979
                     /locus_tag="VGR22_02420"
     CDS             12635..12979
                     /locus_tag="VGR22_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127454.1"
                     /translation="METRAEVDRLQGEIDELRSIIAAGTFTDAEIDRLSAIIGNWIAL
                     DTGLPAAPSEHSAMAQQLQQVRTDLSTVGLNLLLLISAPDSVDINETSQLLDSAERTL
                     DALDQQLTALGG"
     gene            13178..13642
                     /locus_tag="VGR22_02425"
     CDS             13178..13642
                     /locus_tag="VGR22_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127455.1"
                     /translation="MEYLQNRFELLGRSLAILAAYAVIAGAASVAVNLGDVAEGEVVA
                     IVRSALGLVGIAAGLLVWTLRSSLGWQALAAWTLVQVPFIAWSAEGNVTRQMWDILLG
                     FSSSTTVNGVVTSSEQYGLNGVGIILTIWAFRTRERWDQRIAPMLAGQPGMA"
     gene            complement(13843..14490)
                     /locus_tag="VGR22_02430"
     CDS             complement(13843..14490)
                     /locus_tag="VGR22_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127456.1"
                     /translation="MTRIPMQPNDLPATAALCRQTLAPWIDRDWSVPAGDLEWTCRRT
                     LDHIIDTQLFLGGNAAMRSTSRVLPIRNGDPSATVEQLLSAVVTAAMLLERVCAGMGP
                     GGRGFHPAGQADAEGFRAQGCAEILQHTWDIANGLGEGFRAPEDLSGRIVARLFPWTP
                     TADEHPDRWEVLLWSVGRIALPKRERLDSNWWVQMAPLEEWDGQRKVRTMSPAWR"
     gene            complement(14561..15286)
                     /locus_tag="VGR22_02435"
     CDS             complement(14561..15286)
                     /locus_tag="VGR22_02435"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEV2127457.1"
                     /translation="MSARYQRWWARGLARAIDMRIEITGLEHVGDAAYVVTPLHEGLA
                     DALALLHLPVPLRFVLRDEFVDWRLIGGYLRDTGQIAIRPEDGLQAYRSTVRAAQAVT
                     ERGESVVICPQGTVLGIETDFQPGAFAVARALGRPILPVAVTGTHRVWEFPFSPRLRR
                     GVRVGLQVLPPIAAEEVCTTERDTLRLRVQHRVKSAALDRTMPSPRHYVPERDGYWDG
                     YAFDIDPAYPELAANVAAHRDRA"
BASE COUNT         2679 a         5250 c         4985 g         2707 t
ORIGIN      
        1 gcgttgaacg ggtcggcatc ctgctcgtcc atgatcccgt cgttatcgtc gtcgggatcg
       61 acgatatccg ggttgccgtc cccgtcgaag tcgggcttct gatggtcacc ggagccaggg
      121 tcggtgtcgg cggcaacgcc gggccagggg tgggtgtagg ggagacggta acgggagtgg
      181 gcgtcgcggc cgtttcctgc tgcgagcggg tgggatcgag cggataataa tcctgatcgt
      241 cggagacgcc atcgttgtcg tcgtccggat ccatgctgtt cttagtgcca tcgagatcgc
      301 tgtcggcatt gggatcgatg accccttggg cggcgagggt cgataccccg cctgtcagca
      361 gcatcaggac gagacagacc accacgagcg gccggatcgg ccgccgggcc tccgagcgca
      421 tgcgcacacc ctccccctga agcccaccgg cgcaggcggg cccgatccgg ccagcagccc
      481 cggccatccg gatatgtttt ggtattattc cgtctacagt agcagaacct gccgtgtttg
      541 ggacccctct gcggagccga atttaaggaa tgtgggggta tggcacagtc acccgctgta
      601 tcgaactggc cgtcgtgtgc gacgattgac gcgaagacag agctctgccg tcgatggacg
      661 aagaaggtga cagatgacac ggactccact gacggtgatc ccagacccag ggctgacacg
      721 aagcacgctg ctgcgtgagg gcgaggacag catcatcttc tccggtgact tcgaggggcc
      781 ggtgttcacc tgtggcgggt gcgacgcccc actgatcgag ggcgtgcgcc ccacctatgt
      841 cgtggatttg gtgctcttct gcagagcctg cggtgcgttc agtgaatccc ccgcctccag
      901 ccttgcggat atcgacctgg ccaagatcga gcggccagtc atgttcccgc ccgggcggta
      961 cgagttgagc gatgcgatca cggtccacga ccgctgggtg atggcgagcg agcgagccct
     1021 gcaggggacc aggatcgcag cgccggtccg gtacgagacc tagccccggc accggcacgt
     1081 gcggcactgc aagcgcagtg ctgggcgcgg gcatgtcttc cgccaagggt tcgctggacg
     1141 ctcctgaaac ggcgctgcca ttcgcggtag catctgcgga gtgagaccgc gccgtccggt
     1201 tcgccggctg ccattcgcct gccaaggatc caactcgtga ccacttccga ctccaccacg
     1261 ccgtcccggg ctgcgcccgc cgtcgactcc gggcaacgcg gagtgatctg ggacatggac
     1321 ggcactctga tcgactccgc ccgctaccac tacgagtcct ggcgagagct cctcgcgccg
     1381 gagggagtcg atctcgacta ccctgccttc ctggtcggct tcggccgtcg caatgatgat
     1441 tggctccgcg agctgctggg cgcggagatc gcgctggccg agattcgacg tatcggtgat
     1501 gccaaggagg agcgctaccg cgagatgctc cgcgaggagg ggctggtcgt gctgccaggt
     1561 gtgctcgaca cgcttcgggc gctgcgggag gagggctggc agcatgccgt tgccacctcc
     1621 gcgccggtgc agaacctcga gatcatcctc gaggtcaccg gcctgaagcc actgatggac
     1681 ggctgggccg gcagcgagga tgtcgagcag gggaagcccg ctccggatgt ctttctcgtc
     1741 gcagcgaacc acatcggtgt ggcgccgcag cgcgcggtcg tcatcgagga tgccgccgcc
     1801 ggcgtgctgg gcgcgcaccg ggccggcatg tcggtgatcg gagtcggccc gcaccatgcc
     1861 gaactgggtg ccgaccgctc ggcggaatcg ctgcttgatc tccccattga aaccttcgcc
     1921 gagctcgtca tgatggcgtc cagcgcacgc tgagcgcgga aacgaccatg gtacaattcc
     1981 caatcgggac gtgggtcaac gccgacgttc tgccttgcgg cgccatagcc aagtggtaag
     2041 gcacgggtct gcaaaaccct gatcgccggt tcgaatccgg ctggcgcctc cagagccatc
     2101 gagcaacgcg gtcccggcta ccggggtcgc gttcttgcgt ctgggatgcc agatcggatc
     2161 gaccgatccc cacggagtac gcccatgccc ccgatcgaca tctcgaccga caccacccgc
     2221 ccccatcatg aggagcgcat tggcagccgc cgcgcctacg acgggcacat cctccacgtc
     2281 cgtgtcgacg acgtccgcat gccgaccggg cgagagacgg ttcgggagat ggtcgagcat
     2341 cctgggtcgg tgatcgtgct gccggtgacg actgacgatg cggtcgtctt cgtgcggcag
     2401 taccgctaca cggtcgggga ggcgctgatc gagctgcccg ccggactgat cgacgagggg
     2461 gagacgccgg aggtggccgc cgcgcgagag ctcgccgagg agaccggcta cgaagctggc
     2521 gagctgcact tcctgggtgc ggcgtacgtc tcgcccgggt actcgcaaga ggagagccgg
     2581 ttctacgtcg ccacgggctg tagccggggc aagcacgagc cgaatgagga ggagccgatg
     2641 gacgttctcc acgtgcggct ggccgacctg cccgagatgg tgaagccggg cgagacgatc
     2701 atccgcaacg cgcagacgct gctggcggtg aactggttcc tacgcgttca gaataagatc
     2761 atggggacac cgtagcggca gacgacgcag gagggtgccg tggctgcctc gccattccag
     2821 cggaaccaac catcacgtgc agcgagcatc acccgaattg ttgggctcgt ctggatcggc
     2881 ggcgcgatct tcagtgcatt tctattctca ctggcagcgc cctacacctc ggcgacgggc
     2941 ctgtacgcca tcctgctggc ctggcttgcc cgtggagagt accggtcaat tctggcgaga
     3001 tgacacgtga ccaggcacga agagattgta gacacgcgct caggtgcgcg ggtttcgccg
     3061 ctgtttccac ctcgaagtac ctacatcaag tgcgagtcac ccggccaagc cgggtctacc
     3121 tgttgacagc tacgacgagg ttcgcagcat cctatccctt cgtgacctca tggtcggtgg
     3181 tcacgccgag accgtcgaag aggccatcgg cgccggcggg cttccaaagc ggcactgcag
     3241 tcggcgagag atcgatcggc ccggatgcca tctccatgac cttcccctcc tccgccgagg
     3301 ccagcccctg cacgatgatc tccatgttgc gcacgctctg ggcgatggtc ccgacgagct
     3361 ccgcctcgcc cgtcagcgcc tggtggaagt tcaggaactc gttgtagtag cccgcgtccg
     3421 gcatctccac cttccagcac tccgtgctgc cgtccggccg gtggatgcgc accccgccgc
     3481 ggctgatcga catcaccccc tctgtcccgt agagccgcat gtcgttcggc tcctccggca
     3541 tcgccagttc agggtacgac gcggtgtagc tgccgatcgc ctcgctgacg aagtggagat
     3601 tcatcgtcag gtcggacggt ccgttgtgcg tcgagttggc atcctggatc tcgccgctga
     3661 cgtgatcgac atcgccgcag agcagccgga tctgggcggt gttgtggacg ccggcatcaa
     3721 gctgcggccc accgacgtag tctccctccc agcgccacgg tgtgctggag aactcgccct
     3781 cgcgggggat gagctgcgag acgttccgcc atgccatcag atgcatgcgc ccgatcgccc
     3841 cctcgtccag cagcgaccgt gcgaagcgga ggtcgtcccg gtagaaccag ttctcggcga
     3901 tcaggatcgt gcggtcccga tactgtccct ctagatcgat gaacgcccgc atcgcctccg
     3961 cgctgccgcc ggtcggtttc tcgcagatca catgcttgcc ggcctcgagg ctatccttcg
     4021 tgaccgtgta gttgagcggg atcggcagcg agatcagcac ggcgtcgaca tcgtcgcgcc
     4081 ccagcagctc atggtagtcc gccgtgaagc cgtccatcga ggcaccgctg taactgacga
     4141 agtgctccgc gtggtcccgg tcgatgtccg cgaatgtggt gactcggatt cggtcggtca
     4201 tgcgcttcag cgccggccag tgaagctttt caacggcgag cccggtgccg atgatgccga
     4261 gccggacggt gtcgggtgat gccatgctgc tgcccttcct actgatcggt ggccagttgt
     4321 gctgcccaat cggtgcgcag gttcagtgcc agctgccacg cgggctggac atctcacacg
     4381 gcaggtggcg tgaaagttgc ggctgtgcca gttggcgccg ccctcgcgca tacgggggta
     4441 caagaattgc atcgacagtt tgatcgacac gagctctggg agcgatggtg atgatgctga
     4501 acgcgctgct tgccgtgctt ttcctgttcg tgcagacccc ccaggcaggc ctaccaagcg
     4561 acccgagtgc gctcaccgag ttggctgcag agtactgcgc gaccgaggtc gcgcaggcga
     4621 cctatacaaa tgttgagatc gtagaagtcg aagacgtcgg gttgccgccc gtcgtgacct
     4681 acgccgcctt cgacttcgag accgcggagc aggccatcgc cgctctcgaa gaggctcctc
     4741 tcctcgttgt ccaagcctac accgaagacc cagccgtcga gcttgaggat tacgacgact
     4801 tcgccgccga ggccccgaca ccagactacg gtgacgcaga tatcgcccac gtcattaccc
     4861 tcccggtctt ggaagacgaa gacgacgtgc tcgtcatcga gctgctcggc atcgttcagg
     4921 agagccagct actgctggtc ctgatgttca gcgacagcgt cgcctatccc gccgctccgg
     4981 gtgtggcgct ggagaccctc ctgccgttcg gggaggagct cgatgaaacc tgggacggca
     5041 ccggcgacct gcaggacgcc atccccgagg aagacgagat gccgctcaac tgggtacggg
     5101 acgacgtcac cgtcgaggcc cccccgaact gcgagtaaca gaacgggagt cgaatcgctc
     5161 acgagagata tgcagatgcg cacatagcga cgaggagaat ccgccatgat cctgcacatg
     5221 ctgatagccg tgctgctttc cctcacccag gtttcgcaga ctgtcgaatc cgatgatcca
     5281 aacgtgttca tcgacctggg cagcgagcac tgcgccacgg ggatcgtctt cgcagcctac
     5341 gagtacgtcg ttgaggacga agtggccgag acggccaacc ctgcctacat gacctactca
     5401 gcgttccagt tcgagtccgt agaccaggcc gtggcggcat tcgaggatgc cccggtgctc
     5461 gtggcggaaa cgttcagcga tgatcccgac atcagcgatg acccggcctt cgacgaactc
     5521 gtgaccgagg tggtgaccgc ggactacggg gacatctcga acgcctacat catgacgctg
     5581 ccccttgaag gtgaggagcc cgacatcctg acgatcgaga tgctgagtat cgtggaggag
     5641 gcccagctgc tgctcatcct gatgttcagc gaaaccggca ctacccgacc gagtgtcggt
     5701 ctgaccctcg agaccgttcc gcccttcata ggagatctgg acgagcgatg ggagggcatt
     5761 ggggatctgg aggaggcggt ccctgccgaa gacgagatgc cgctcggctg ggagggcgag
     5821 cgggtcattc tcgaagatcc cccggactgc tagtgccgac gaacgccggt gaatgacagt
     5881 ggtgaacctc taaaatacag agacgccccg cccgccctgt cgagcgacag ggcgggcgtc
     5941 tttgcatcga cggacctttc agccgcaaca ggccgcatca gactcaatcc gtaaggtgta
     6001 tgatcagtcg gaacaccctc tcttttctcg catctgtgct tttacaaaag aggcgatgaa
     6061 gtggtgtgct cgccggctca tcttggtcgt cctccgtctc cccggaaggt tgaggcaccc
     6121 cctcggcgcc ggtgtcaaga cagccgtgtt cgtgctcaag gtgctgccga cgctgccgtc
     6181 ccgcccggtc gactgggtga ctcccccgcc cgtccgtgag cgggtgcgct acccgtcgct
     6241 gcacggagag gtggaggccg agatcgcccg ccccgcctca cgcggtccgc accccggcat
     6301 cgtggtctgc ctcggcgttg tccccttcgg cgtcgatcac ccgcaggttc ctcgtctcca
     6361 aggggccctg acccgcgccg gcttcgccac cttgctctac tggtctccca acatgcgtga
     6421 ctggcgcctg gatccggttg acgtcgagga cctggcgatg gcatacgagt ggctggtggc
     6481 gcaaccgttc gtggacccgg aacgcagcgg gctgttcggc acgtgtgtgg gcgggtcgtt
     6541 tgccctgatg gcctccgccg atgcccgcat ccgcgatcgc ctcgccttcg tcggcgcatt
     6601 ctcgccctac gcctcgatgc ggacactcac ccgggacatc gccacgggga cgtggctccg
     6661 tgagggtggg cgtgaggtgt agccggtcga tccgcttacc cgcaaggtct acgtgcagtc
     6721 gctgacggca ggtctcgacc ccgccgaagc agcccgactg cgcgaggcga tggccgagcc
     6781 ggccgggcag gttgacccag ctgcgctctc gccggcggcc cggacggtct acccgctgct
     6841 cacccggttg accatcgacg aggtcgagtc agcgaccgat gcgctaccac aggcgctccg
     6901 tgatcgcctc gatgcactgt ccccgatggc ctacctgcca aacctgcgct cgccgatcat
     6961 cgtgctgatg cacgcccacg acgactcggt gatcccacgg actgaatcgc tgcagttgcg
     7021 ggacgccctc gccgatcggc cgggacttcg gtataccgag ttcatcatgt tcaagcacct
     7081 cgaccccagc aaggtccaga tgcgcatctt gcccctgctc cgcgaactcg gtcggttcta
     7141 ccttgccctg tatccgatgt tccgaggtgg caccggttga cccctgccgg actacatccg
     7201 gaccctgagg cgcttctcgg tctccaccag cgtcgtgccg tagacggcat gggcgacgat
     7261 catcaccacc gttcgggatg gtcggtcgtc gtcgggccac gggtagaggc gcaatgccgg
     7321 caggtatccc aggtaggcgg tagtccagac caccgcgccc cagaccagcc ccgcgagccc
     7381 gctcgacccg ggtagaaacc gtcgcaccgc gacatacagc attcccacga ccacaccgta
     7441 gccgtggtgg gtaaccagcg tgccggctgt gaactccggt tccgggctcc gggaggtcat
     7501 ccctacccgt cgcgtgacgt tcacggccac ctgctccggg ggcggcatgc ggagcaggcc
     7561 gaccagcttg ccgatctcga tggcgatcgt catgatcgcc gttgcgatca ccccggcgac
     7621 cgcaccgatc agatagcgat tcatcggaac atgcctccct cccgttaccc caactgggcg
     7681 aaatgctcat taacaaccat cgtcgcaaga ccggcaccat ccattgccac ccgggaccac
     7741 gcagcctcgt tccagctgtg ccttccagtg atcagggtgc ggagtggccc ggacggctcg
     7801 tagactgagg acatgactgc ctcgtccgac gcccccacca ccccatcgtt cggctggcgc
     7861 cagctgctcc tcaaccgaga gcacctcgcc tttgcactgc ccggcttcat cgggcgcctg
     7921 ccgctggcga tgcgggcgct cgggtgtgtc ctgctgatcc agggcatcac cggcgagtac
     7981 ggcctcgccg ggctcgtcgg ggccgcgcag acgctggtga gcgccttcgc atcgccgcgc
     8041 atcggccagc tcgccgatcg ccacggcacc cgcccggtcc tctccatcgc gctcgtgatc
     8101 cagacgatcg gcgtgatcgc gctgatcacc accgcctacg ccagtgcacc cgcgccggtg
     8161 ctgctgctcg ccgcagcggt gattggcggg tcgtcggtcc cgttcgggtc gttctcccgc
     8221 gcacggtgga cgcagcacgt ggcccgaggc cgtgacctcg atcgcgccta tgcactggag
     8281 ggtgtcttcg aggaaatctc tttcatcgtc gggccggcgc tggtcgtgtt cctctccatc
     8341 gagatcgcgc cgccgctggg gctgatcgtc gcgctgctca tgaccttcgt cgccagcgcc
     8401 gggctgatcc gcctgccgga gatcgcgcac cacgccatgc aggcaccgga cgcgccgcgc
     8461 acgtcgctgc tccgcaaccc ggtcatcctg gtcgtcgccg gcagtggcat cggcatgggt
     8521 attctcttcg gcgcgatcga gatatcgctc gtggccttcg ccgaagagct gggccgcgag
     8581 gccacggcga gcgccctgat cgccatcttc acggtcggca gccttgtcgc ggcagtgctc
     8641 tacggcgtgc tcacgatcag ccacccgctg cgttggcggc tgctggcctc ggtcgtctgg
     8701 gtggggctgt ggatgatccc gaccgcgctg gcggagtcgc tccccgcaat gatcgtctgc
     8761 gtgctcttcg ccggaatcgg catctcaccg tgggcgatct ccggcagcag catgatcgag
     8821 cgcggtgtcc ccgcgcacac gttgcgggag gggttcggct ggttctcggc ggccaccgcc
     8881 agcggcgcgg cgctcggggc aacgctcgcc ggcatggcaa tcgaccgatg ggagtcggac
     8941 ggcgggcagg tggtctcgat cctcggcggg atgctggcga tcgcagtcgc gctgctcggg
     9001 cagcggcgtc tcagggaact ggctcctggc tctatgccct tgtgaaaggc cgaacccttt
     9061 cgcctgcgcc ttgctgaggg gttgcactct cgcacacgtc tgcgggctca ccgaccttac
     9121 cgccgaactc cgcggcccag taccagtcgt atttcgcctc gggattgtgg gcccggccga
     9181 tcccgaccgc ggtgaactgc tcgccaagca tgatctcccg gtgctcgggg gagttcagcc
     9241 actgctcgaa ggttccctcg gcgcttcgct ggccggcggc gatgttctcc ccgatcgcgg
     9301 tgttgtaggt gtaccccacc tggtcgagca gctcttcggg cccctgcccc tccggcgaga
     9361 tatgggccag gtagttgtcc tgagccatgt cgcgcgcctt cagctcggca gccctgccga
     9421 gcggctgcga gagttggagg ggatccaacc cgcgctcctc gcgaaggtca ttgatacgtc
     9481 ggagcagctc ggcctcggcc tcagtcgcac aatagggagc ggtatccgtt tcctcgtcct
     9541 gagctgtggc caggcgtata cccccgctca ccacgagcac ggccaccagg cccacgatca
     9601 tcgacaccag aaccctattc gtcactcgag tcctcctttt cacgatatgg gatcgcagaa
     9661 gcgatgccac ttggtgaagg agagtcgcca tctcgcacat tctccgatgt ttatcgaggt
     9721 accccgaaag cacgaacctt ccaagacatg cctgtcactc actgcgtaca ggtgcctggt
     9781 ggatccgaac gcacttcagt gacaggcagc cggtatcgtt gccgaatgac acataccacg
     9841 caccggcatt gcaggaaggc agttaagaac ttgcgccaat aggcgcaagc ggtatcggtg
     9901 gccgactggt ggccgccact gatggcggga gtgttgcgga cgatgccgcc aaacaacctc
     9961 tccctattgg cgcaagctct aagccataca cgcctcctgc cgctcaaatg gtctactcat
    10021 gcatagccgc cgaagtgccg gtgatgtctg ggatcgggtc ttggtggtgc agatggggca
    10081 gctcgcacag acgaccgggc ggcgcgactc ttgcgatgac atcccctgag tcgtatctaa
    10141 aactgcaagg ggatgagtca tggggtgtct aacacgaatc gtaaagggaa tcctgatagg
    10201 caaggctatc cagtggctac gccgacggtt gtaggatcgg aggcggccga gtcgatccga
    10261 atcacgcctc ggcaagatac aggggcccac cggtatgcca tgaccatagt cgaactgaga
    10321 atcccatacc cttggctcca gccgatagcg agaacaccgt gacagcgcgc aatatcccgc
    10381 ccgccgactc tccagcgctg tgcgatacta cggacgaact gccccatcaa cggcgatgcg
    10441 tcgcagcacg atgaccagac atttcactcc accacgcaca ggagcaccag caccatggcc
    10501 aggttcgcgg agaccatgcc gtacgccttc ttcgagggcg agatcgtgcc gtttggcgag
    10561 gccaacgtca gcatcggcac gcatgcgctg caatacggca ccggcgcctt cgctggggta
    10621 cgcggctatc tggaccagga cggcaagacg atcaacatct tccgcctgcg cgagcacgcc
    10681 gcacgcctgc tgcgatcggc acggctcctg cgcatggacc tcccctatga tgccgacgcc
    10741 gtcggccaga tcctcatcga cctcgcccag aagaacgcgc ccagcagcga cgtctacttc
    10801 cggccgttcg tctacaagtc ctcggtgcag ctcacgccac gcctgcaggg catcgacgac
    10861 cagctggccg tctacatgat gctgatgggc gactacctcg acaccaaccg cggccagaag
    10921 gcgatcgtct cctcctggac gcgcatctcg gacaacgcga tcccgagccg aggcaagatc
    10981 accggcgcct acatcaactc ttcgctcgct aaggacgagg ccgaggagaa gggcgccgac
    11041 gaggcgatca tgctcaccgc cgacggcacg gtcgccgagg gcagcggatg caacctcttc
    11101 atcgtccgtg acggcgtgct ggtcaccccg cccatcacca gcgacatcct tgagggcatc
    11161 acccgccgct cgctgctgaa gatggcgcag gacctcggca tccccatcga ggagcgcggc
    11221 attgaccgct ccgagctcta catcgtggac gaggccttct tcgcgggaac cggcgtccag
    11281 atcgcgcgga tcgacactat cgacggccgc cagatcggca cgcggggcga ggcgccgatc
    11341 agcaagcggc tcagcaaggt cttcttcgac accgtccgcg gcgcgaacga cctctaccgc
    11401 gactggatca ccccggtgca gattccggtc gaggccgcgt gtcgctagcg tctgcacttc
    11461 atcatgcatc gcgccgaggg cgggttccac aggggacccg ccctcgctca tactccgctc
    11521 atactcttgg gggccagttt gcggccaaac cgctgcatgg ctctgctaga gtgaaaacca
    11581 cgcactggtt acccgacgag ttcaatgcca tcgtatgcgg ctgcggcgca gctgcaaccg
    11641 tgtacagaag ggaacgtccg tcatggtgtc gttcatccgc ctcgttctgg gactccttgt
    11701 agcagtcgcg agcgcaacgg cgctcccatt ggcaacgact gccgcacagc aacccagctg
    11761 tgccgactac tcgaccgcgg cagccgccca gttcgcgctg gacatcaacc ccgtcctcgc
    11821 ctccgcgctc gatcccgacc gcaacggcgc cgcctgcgat catgaagacg ccagtggcac
    11881 cacagacggc gctgggggac tgcagctgcc agaaacgcag gccccggccc agatgccggc
    11941 ctcgaacgcc ccggccgagg tgaccgtcga cgcgatcgac cagcagccag cggacaccac
    12001 cacgatggcg acgcagccct ccaccacgca gcaggcggga gtgctcgacg gtcggctcgg
    12061 cggcagccag gcggcgttcg cggcggcgca tggtcagccg atcgaggagg tcccaagcga
    12121 caccaacccg gcagtcgtgc tgcatgccta cccaccgccg tcgacggccg tcgacctcta
    12181 caccatcgac ctgaacgacc gggtcgctat cgtcgtggtg gccgccgaga cctcatgggg
    12241 ctcccagcag gcggcggacc tcatcgccga ctaccttccc cccgatgtca ccgagctcgg
    12301 tgacgtggag ccactcgcgg acggcagtct gctgatcccc atcttcagcg aggatctcgc
    12361 aaccggggtc agcgccgcga cgatgacaca ggccgtcatt tccggcgtgc cgggtgatct
    12421 ctatctgttg cttttcccgg acgatgcgaa cggcgtcgtg gagatggaga tcgggctcgg
    12481 caacggtgac gatctgcgcg aggacgtgca gacgtctggc ccggtgcccg agccgaccgc
    12541 gacgcagcag gccaccggcc cgggcctggc accgacacaa actcccagca attagactga
    12601 cctgagtgcc gccagcgatc cgaccacctt tctggtggag acgcgggcag aggtcgaccg
    12661 gctgcagggg gagatcgatg agctgcggtc catcatcgcc gccggcacgt tcactgacgc
    12721 ggaaatcgac cggctctcgg cgatcatcgg gaactggatc gcgctcgaca ctgggctgcc
    12781 ggctgccccg tcggagcaca gcgccatggc ccaacaactc cagcaggtgc gaacggacct
    12841 ctcgacggtc ggcttgaacc tgctgttgct gatcagcgcg ccggacagtg tcgacatcaa
    12901 cgagacgtcc caactgctcg attcggcaga gcgcaccctc gatgccctcg atcagcagct
    12961 caccgccctc ggtggatgat catccccctc gggggatgat catccacccg gtcgctatga
    13021 tgatctgggt ccccggtttc atgctgccga acacgggtca ggtgacgtgc gcgctgagga
    13081 gcatagtgct cgaaccgctc gagccggcac cgatcatcct ctgtatctgg cagcggaacc
    13141 gactgactgt ttcgcacgac agacatgagg aagcgttatg gagtacctgc agaaccggtt
    13201 cgaactgttg gggcggtcgc tggcgattct tgccgcctac gcggtcatcg cgggagcggc
    13261 aagcgttgcc gtgaacctcg gggacgtcgc cgagggcgag gtggttgcca tcgtccgctc
    13321 ggcgctgggt ctggtcggga tcgccgccgg cctgctcgtc tggacgctgc gctccagcct
    13381 cggatggcag gcgctggcgg cctggacgct cgtccaggtg ccgttcatcg cctggtccgc
    13441 tgagggcaac gtgacgcgcc agatgtggga catcctgctg ggcttctcca gctcgaccac
    13501 cgtcaacggc gtggtgacct cgtcagagca gtacggcctg aacggcgtcg gcatcatcct
    13561 caccatctgg gcgttccgaa cccgcgagcg ctgggaccag cgcatcgcac cgatgctcgc
    13621 cggccaaccc ggtatggcct gatcgaaggc gcatacttcc aaccgaccgt cctgtcattt
    13681 cgaccgaagc agccgcaggg tgcgacgtgg aggaatctct gactgaccgg ccgcaagcgt
    13741 ccggcaaggg agagacttct cgcctgcatc ctcactgcgc tcggattccg accgaaatga
    13801 ctcgctgtgg agaatagggg tggggaaagc acccgaactc cctcaccgcc aggccggcga
    13861 catggtgcgc accttccgct ggccgtccca ctcctccagc ggcgccatct ggacccacca
    13921 gttggaatcc agtcgctcgc gttttggcag cgcgatgcgc ccgacggacc agagcagcac
    13981 ctcccagcgg tccggatgct cgtccgcggt tggcgtccat ggaaagagtc gggcgacgat
    14041 gcggccgctg agatcctcag gcgcccggaa cccctccccc aatccgttgg cgatgtccca
    14101 ggtgtgctgc aggatctcgg cacatccctg tgcccggaac ccctcggcgt cggcctgtcc
    14161 ggctggatgg aagccgcgcc cgcccggtcc catgccggca caaacccgtt ccaacagcat
    14221 cgccgcggta acgaccgcgc tcagtagctg ctccaccgtc gcactggggt cgccgttgcg
    14281 gatcggcagc acgcgggacg tcgaacgcat cgctgcgttg ccgcccagga agagctgcgt
    14341 atcgatgata tggtccagcg tgcgccggca ggtccattcg aggtcaccgg caggcacgga
    14401 ccaatcacgg tcgatccagg gagccagtgt ctggcggcag agtgcagcgg tggctggcag
    14461 gtcattgggc tgcatgggga ttcgggtcat gggacatgct cctcacggcg atgggtggag
    14521 aaccgtggat acccccatca tcgcgagacc atgacgatcg tcaagcgcga tcccggtgcg
    14581 cggcgacatt ggcagccaac tccggatagg cgggatcgat gtcgaatgcg tagccgtccc
    14641 agtagccgtc gcgctcggga acgtagtgcc ggggcgaggg catcgtccgg tcaagagccg
    14701 cggacttgac ccgatgctgc accctgagcc tgagggtatc gcgctccgtc gtgcagacct
    14761 cctctgcggc aatcggaggc agcacctgca ggccgacgcg tacgccgcga cgcagccggg
    14821 ggctgaacgg aaactcccag acgcgatggg tgccggtcac ggcgacaggc aggatcggcc
    14881 gtccgagcgc gcgtgcgacg gcaaaggcac cgggctggaa gtcggtttcg atgccgagaa
    14941 cggttccctg ggggcagatc accacactct cgcctcgctc ggtgacggcc tgggccgcgc
    15001 gaacggtcga ccggtaggcc tgcagcccgt cttccggtcg gatggcgatc tgcccggtat
    15061 cgcggaggta tccgccaatc aggcgccaat cgacgaactc atcccgcaac acgaatcgga
    15121 gcggcaccgg cagatggagc agcgcgagcg catccgcgag cccctcatgg agtggcgtga
    15181 cgacatacgc cgcatccccg acatgctcca gacccgtgat ctcgatgcgc atgtcgatcg
    15241 cacgcgcgag cccgcgtgcc caccaccgct ggtaccgtgc ggacatccgg tggtaccgtg
    15301 cggacatccg gtatgcgcca gcacgggaga ggagtcgcgc cggcagaggc actgccagca
    15361 cccagatccc gaactccgcc agcgtccgaa ggtatcccaa caaggtcgat gcgccgatcc
    15421 gcccaccgag cagtatgtac cggatcggag ggcccgcccc ctgctcgtcg catgccgcga
    15481 cgcgaggctg gcttcggacc cacgccacct acctgcgctc cagcacggcc gcggtgacga
    15541 ggccgtaagc gagatgcggg atgaggtcgg aaagccaggc ggagcggctc caggtccgcg
    15601 ggtcgccgtt acccgtcagg a
//
LOCUS       DASYWL010000069         9856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_209368, whole genome shotgun sequence.
ACCESSION   DASYWL010000069 DASYWL010000000
VERSION     DASYWL010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9856
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209368"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1668
                     /locus_tag="VGR22_02440"
     CDS             <1..1668
                     /locus_tag="VGR22_02440"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV2127458.1"
                     /translation="TGSGEARAIFLGGMGEVGKNVLLVETDDDIAVIDFGLGFPDETQ
                     HGVDVVLPDVSYLRERREKIRGIYITHGHEDHIGALPYLWEAIGAPIYATRLSAGLIQ
                     GKLQEARLDKKTEVKVFDPDERPVLDAGSLRIEPFRVTHSIPDAVGFGIQTPAGLIVH
                     TSDFKFDPTPVDGKPTDYGAIEAFGKRGVELLISDCVHIESTGATPSEAVISETFDAV
                     FAAAPGRIIVATFASQIARIQQVIDVAGRYGRRVAPLGRSLTRNVAIAQELGYLHDPD
                     NVLIDDRDAHNLPEDEVVYVVTGSQGEPMAVLSRIANQEHYEIRVHDKDMVILSATPI
                     PGNETSVYRIIDQLFHQGAEVIYSARAHVHVSGHASRDELRRMIEMTTPRHVLPTHGE
                     FRHLALYADLAREAGIPDERIHMVALGDVVTLSGGQVAVTDHIETGRIFVEGRTMNTV
                     DAETLSERGQMANEGIITIVVPVDLVGERIAGEPAITATGLLMQLEDAVRGEMMVALR
                     ESIEGMLEKGTNDPARLEKRVASIAASYIYRGHRRRPKIQPIILDVS"
     gene            2212..4311
                     /locus_tag="VGR22_02445"
     CDS             2212..4311
                     /locus_tag="VGR22_02445"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEV2127459.1"
                     /translation="MPAVEISERLRREILGVALVILALVTSWALGRGAGDGAIISWWS
                     SALRTLLGDAAPLVPVFLALTAIRAFANYGQPVLLFRHYIGGIMFATAILGLMSFDGP
                     ERAGEVGRFIGDIVEGALGFVASGLVLLAIGVVSVFLLADTDIQTLERDIRRLRPKRR
                     PKAEEPRKPVIGGEVTSGKRRKKVKKALPVVDAEPVQRIINIPKPSSPPAKSSAKEMP
                     RVVTQPVEDDEPPPLPDTRLLSYYDQVVPDKQELEVKAKVIEETLRSFKVQARVREIN
                     PGPAVTQFALEPGNGVKVRRISELHNDLALSLAAQSVRVEAPIPGQARVGVEIPNKQI
                     ATVGLREVLESQVFQTGKYKLPLPLGRDVNGRAVVGDLAKMPHLLIAGATGSGKSVCL
                     NGVICTFLMQKRPDELRMLMIDPKMVELTGFNGVPHLLCPVVTEMEKVVGALRMVLKE
                     MNRRYELFKSLGVRNLDGYRKKTANDETAEKIPYLVVIIDELADLMMTTSDEVETLLA
                     RLTQMARATGIHLIIATQRPSVNVLTGLIKANVPARIAFAVSSNTDSRVVLDQPGAER
                     LLGRGDMLYLPGDAAKPVRIQGVFIEDEVAAIVNHWHTVSPVPQYDEEWLDLPTNEVQ
                     TESGGGYDDDEDPLMEEALGVVRSQKLASASMLQRRLRIGYNRAARIIEQMEADGVIG
                     PADGAKGRPVLVGFEDE"
     gene            complement(4431..4766)
                     /locus_tag="VGR22_02450"
     CDS             complement(4431..4766)
                     /locus_tag="VGR22_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEV2127460.1"
                     /translation="MQFEDKTLRCKECGDHFTWTAGEQLFYREKQLINIPARCPKCRG
                     TRKAKLGLPDRAMTEVVCADCGQTTTVPFVPRNGNPVFCSMCFERNRQRERTAANEQE
                     LATSGSHQT"
     gene            5082..5696
                     /gene="recR"
                     /locus_tag="VGR22_02455"
     CDS             5082..5696
                     /gene="recR"
                     /locus_tag="VGR22_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEV2127461.1"
                     /translation="MALQPRTGVTAQPVSRLIDELSKLPGIGPKTASRLAYHILKTPK
                     DDALGLAEAILEVKERITLCSVCFNTTDIDPCAICANEGRENRILVVEQPLDVVAIER
                     THEYKGRYHVLHGAVSPVDGIGPDKLRIQELVDRVQQRLPDEVIVFTNLDLPGEATAT
                     YLHRILTPMGIRVTRPASGLPVGGDLEYADDQTLGRALSGRREL"
     gene            5741..6922
                     /locus_tag="VGR22_02460"
     CDS             5741..6922
                     /locus_tag="VGR22_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127462.1"
                     /translation="MIETPRSTIDRHRSVITRSSQRAPFHLLSPTGRREPGESRASTP
                     RYAPGYDPDLTAGRPALSDLPALVTAPRFPLDLARRDHSAQWLADRAIRERLGLFRGN
                     VRMRVSRRAGCLAAAAAFRVLAPDWRWQLEALVACNPEDGAALDTALRSGVKDAGEAG
                     ARRVLARLPGDPTVDSAMRRAGFVPFVTEFVYVLADDRPGRPSGAPYVRRVHPADVWG
                     IHQLYLDVVPRQVQYAEAVTSRTWERSRPLRPGTRRSSGWVIEDHGRVRGYARVSTEI
                     DPGVVRIDLLIDPLLRTLAPDLISAVVSEAQQQHGVPCVVVIPGYAQELHQPVQEAGF
                     RHVGDQTAWVCYTTVPARSYIVAVDLGAPAAAEDQRARVPGFGGAGMSVTGPVEWGNP
                     A"
     gene            7013..8854
                     /locus_tag="VGR22_02465"
     CDS             7013..8854
                     /locus_tag="VGR22_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HEV2127463.1"
                     /translation="MSTAIHTPPTVQHVGAEYAVTDNLTDMLAVLPSRIAAAIREHEN
                     MHVDGGLIEVVMDLGRPAEMRYQQGEITLDDFEVTREDLNAVADRIGHFGADNRAGIE
                     RTLHRISAIRNRTGDIVGLTCRVGRAVYGTIAIITDLVESGESVLLLGRPGVGKTTML
                     RETARVLADDLRKRVVIVDTSNEIAGDGDIPHPAIGRARRMQVPTPEAQHAVMIEAVE
                     NHMPEVVVIDEIGTELEAGAARTIAERGVQLIGTAHGNTLENLMLNPTLSDLVGGIQS
                     VTLSDEEARRRGTQKSILERKAPPTFSAMVEIVDRDEVIVHRDVAATVDSILRGVTPP
                     SEKRRRREDGTIESASGDDARRDPTGRSSSGSGGPSLTSIAGNASAGQRGSRDVDFGS
                     SPRAPRAQEAAVRHNLTETANGQSVQETSEPAPVDPRSPFSRGRGTRDKPLRVFPYGV
                     SRNRLHQSITRNRYPAQLVKDARDADLIVTLKNVFRRKPELLRNAETTGTPVYVSRSN
                     SAAQLDDVLRNLFPGIEIEEETELAPSTVPAGFNGGNEHRMMDALNEAESAILRVIDG
                     GPPESLEPQSVGIRRKQHELAERYNLASRSRGKDPFRHVEIYRQGAQ"
     gene            8854..9516
                     /gene="tmk"
                     /locus_tag="VGR22_02470"
     CDS             8854..9516
                     /gene="tmk"
                     /locus_tag="VGR22_02470"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEV2127464.1"
                     /translation="MSAGTFVVFEGPEGAGKSTQMRRLADVLVRAGHDVVATREPGGT
                     PVGDAIRRVLLESDGYAMLPETEAFLLSAARVQHVHEVIRPALDRGAVVLCDRYADST
                     LAYQGGGHGMAEGDLRCLERIATDRLVPDLRVLLDVPAAAGLARRHRDTTSVNRIDRA
                     SLAFHERVAAAYRLLAERDPEGWTVIDATQPTDAVFTMVCQAVQSSLGFSLEQAGGQC
                     QV"
     gene            9524..9853
                     /locus_tag="VGR22_02475"
     CDS             9524..9853
                     /locus_tag="VGR22_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEV2127465.1"
                     /translation="MKLVIAVVQGKDVSELLSALTAAGYRATQISSSGGFLRESNVTL
                     LIGVEDHEVTSVQAIIQENCHSRTHFVNPLMPIVEPGESLMPAPVEVPVGGATVFVLR
                     VERFERL"
BASE COUNT         1746 a         3064 c         3264 g         1782 t
ORIGIN      
        1 acgggcagtg gcgaggcgag ggcaatcttt ctcggcggga tgggggaggt cggcaagaac
       61 gtcctgctgg tggagacgga tgacgacatc gccgtcattg acttcgggct cgggtttccc
      121 gacgagacgc agcacggagt cgacgtggtg ttgccggatg tcagctactt gcgcgagcga
      181 cgcgagaaga tccgcggcat ctacatcacc cacggacacg aggaccacat cggcgcgctg
      241 ccatatctct gggaggcgat cggcgcaccg atctacgcca ccagactcag tgccggtctc
      301 atccagggca agctgcagga agccaggctt gacaagaaaa ccgaggtgaa ggtcttcgat
      361 cctgacgagc ggccggtgct cgacgccggt agcctgcgta tcgagccgtt ccgcgtcacc
      421 cacagcatcc ccgatgccgt cgggttcggc atccagacgc ctgccggcct gattgtccat
      481 acgagcgact tcaagttcga cccgacgccg gtggacggca agccgaccga ctacggcgcg
      541 atcgaggcct tcggcaagcg tggcgtcgag ctgctgatct ccgattgtgt ccacatcgag
      601 tcgaccggcg cgacgccgtc ggaggcagtg atcagcgaga ccttcgacgc tgtcttcgcc
      661 gccgcgcccg gccggatcat cgtggccacc tttgcctcgc agatcgcccg tatccagcag
      721 gtgatcgacg tcgctggtcg ctacggtcgg cgggtcgcgc cactcggccg tagcctcacc
      781 agaaatgtcg ccatcgccca agagttgggg tacctgcatg atcctgacaa cgtgctgatc
      841 gacgaccgcg atgcccacaa cctgccagag gacgaggtcg tctatgtcgt tacagggagc
      901 cagggcgagc cgatggccgt gctgagccga atcgccaatc aggagcacta cgagatccgg
      961 gtccacgaca aggacatggt tatcctctcg gcgacaccga tccccggcaa tgagacatcg
     1021 gtctaccgga tcatcgacca gctctttcac cagggagccg aggtgatcta ctcggcgcgg
     1081 gcgcatgtgc atgtctcggg acatgccagc cgggacgagc tgcggcggat gatcgagatg
     1141 accacgccgc gacacgtgct gccgacgcat ggtgagttcc ggcatctggc gctctacgcg
     1201 gatctggccc gggaggccgg catccccgac gagcgcatcc acatggtcgc gctgggcgat
     1261 gtggtgacgc tttccggcgg acaggtagca gtgaccgacc acatcgagac cggacggatc
     1321 ttcgtcgagg ggcggacgat gaacacggtt gatgcggaga cgctgtccga gcgcggccag
     1381 atggccaacg agggaatcat taccatcgtt gtgcccgtcg acctggtggg cgagcggatc
     1441 gccggggagc cagcaatcac ggctactggg ttgctgatgc agctcgagga cgccgtgcgc
     1501 ggcgagatga tggtggcgct tcgggagtcg attgaaggga tgctggagaa ggggacgaac
     1561 gatcccgcgc ggctggagaa acgggtggcg agcattgccg cgtcctacat ctatcggggg
     1621 caccgtcgtc gcccgaagat ccagccgatc atcctcgacg tctcataggg gcaggacagc
     1681 catcagtgta gtcatcctga tccctggccg gagttgaagg atttcgtgcg tcccggtcgt
     1741 caacgcgcga gcctccgtat caatcatcca agagatttgt cgacgcgccg attacgttgc
     1801 agatacatgg cgacaggcat gacatctctt cgttgtccct cagcagctcg ccttggcgtt
     1861 cgcggtatct gaacccgggt ctacaggctg tcgggtgttc cggtggctcc cgaaccggtg
     1921 ccgatatcat tggacgaaca cccctgacac acgaccgcat cggcgtgtat actagtgtcc
     1981 gtacactcct gcctgccaca ggtggagctt tgccgactcg ttggcgccgt acggcggcat
     2041 tccgttgtac ccgcgtgtac tggaaagggg agagtaatgg ccgcaatccg atcacgctct
     2101 ggtggagctc cgggccgttc ctcatccagc tcgccttcgc gcaggaagaa gtcgcaaggc
     2161 accgttgtca gacgctggta ccgagctacc cgtgattttg tcagtggcct cgtgcccgcc
     2221 gtcgaaatat ccgagcggct gcgtcgcgag atcctcggcg tcgcgctcgt gatcctggca
     2281 ctggtcacct catgggcgct cggtcgcggc gcgggcgacg gtgcaatcat ttcgtggtgg
     2341 tccagcgcac tccgaacact gctcggtgac gcagcgccgc tggtgccggt cttcctggcg
     2401 ctgactgcca tccgggcgtt tgccaactac gggcagccgg tgctgctctt tcgccattac
     2461 atcggcggca tcatgttcgc gacggcgatt ctcggcctga tgtctttcga tggtcccgaa
     2521 cgggccggcg aggtgggcag gttcatcggt gacatcgtcg agggcgcgct cgggttcgtt
     2581 gcctccgggc tggtgctgct ggcgatcggc gttgtctcgg tcttcctgct ggccgacacc
     2641 gacatccaga cgcttgagcg cgacattcgg cgcctgcggc ccaagcggcg cccaaaagcc
     2701 gaggagccgc ggaagccggt gattggcggc gaggtgacca gtggcaagcg tcgaaagaag
     2761 gtgaagaagg cgctgccggt cgttgacgcc gagccggtcc agcggatcat caacatcccg
     2821 aagccgtcct cgccgccggc caagtcgtca gccaaagaga tgcccagggt cgtgacccag
     2881 ccggtcgagg atgacgagcc gccgccactg ccggatactc gcctgctttc gtattacgat
     2941 caggttgtgc cggacaagca ggagctcgag gtcaaggcga aggtcattga ggagaccttg
     3001 cggagcttca aggtgcaggc gcgcgtccgc gagatcaatc ctggtcctgc cgtgacccag
     3061 tttgcactgg agccgggcaa cggcgtgaag gtgcgccgaa tcagtgagct gcacaacgac
     3121 ctcgccctgt cgctggcagc ccagtctgtg cgcgttgagg cgccaattcc cggccaggcg
     3181 cgcgtcggcg tcgaaattcc gaacaagcag atcgcgacgg ttggcctccg tgaggtgctg
     3241 gagtcgcagg ttttccagac cggcaagtac aagctgccac taccgctcgg acgggatgtg
     3301 aatgggcgcg ccgtggtcgg cgatcttgcc aagatgccgc acctgctgat tgccggtgcg
     3361 acaggctccg gcaagagcgt ctgtctgaac ggggtgatct gcaccttcct gatgcagaag
     3421 cgcccagacg agctccgcat gctgatgatt gatccgaaga tggtcgagct gaccggcttc
     3481 aacggtgtgc cgcatctgct ctgcccggtc gtcaccgaaa tggagaaggt ggtcggggcg
     3541 ctgaggatgg tgctcaagga gatgaatcgg cgctatgagc tcttcaagtc gctaggcgtg
     3601 cggaacctcg atggctaccg aaagaagacg gccaacgacg agacagcgga gaagattccg
     3661 tacctggttg tgatcatcga tgagctcgct gacctgatga tgacaacctc cgacgaggtc
     3721 gagacgctgc tcgcgcggct gacgcagatg gcgcgcgcca ccggcatcca cctcatcatc
     3781 gccacacagc ggccgtcagt gaacgtgctt acggggctga tcaaggcgaa cgtcccagca
     3841 cgcattgcct tcgcggtctc gtccaacacc gacagccggg tggtgctcga ccaacccggt
     3901 gccgagcgcc tgctgggacg cggtgacatg ctgtacttgc caggcgatgc cgccaagcca
     3961 gtccgtatcc agggcgtttt catcgaagac gaggtcgcgg cgatcgtcaa ccactggcac
     4021 acggtgtcgc cggtgcccca gtacgacgag gagtggctcg acctaccgac caatgaggtg
     4081 caaacggaga gcggcggcgg atatgacgat gacgaagacc cgttgatgga ggaggcgttg
     4141 ggggtggtgc ggagccagaa actggcgtcg gcgtcgatgc tgcaacgtcg gttgcgcatc
     4201 ggctacaacc gggctgcgcg gattatcgag cagatggagg cggatggcgt catcgggccg
     4261 gccgacggtg ccaagggccg accggtgctt gtcggattcg aggatgagta gcggcgcagg
     4321 agctgcacgc cgcggttgaa cgtttctgcg tcgctccagc ggcgacggca gaccgccgcc
     4381 ggatatctcc ggcggcggtg cattgattca cgataaccgt gatcgacggt ctacgtctgg
     4441 tgactccctg atgtcgccag ttcctgctcg ttggctgctg tccgttcgcg ttgcctgttc
     4501 cgctcaaagc acatcgagca gaagaccggg ttgccatttc tcggaacgaa tggaacggtt
     4561 gtcgtctggc cacagtccgc acatacgacc tcggtcatcg cacggtcggg cagtcccagc
     4621 ttcgccttgc gcgtcccgcg acacttcggg cagcgagccg gaatgttgat gagttgtttc
     4681 tccctgtaaa agagttgctc acctgcagtc cacgtaaagt ggtcgccgca ctccttgcaa
     4741 cgtaacgtct tgtcctcgaa ctgcacgttg tcgaccctcc tcttggtagc gatcgcgtgc
     4801 gttcattcga cagatactgt ggacattcca cggcgcttcc gaaccgacgc cggccacacc
     4861 actgttggac ggacgaatga aacacagcct tcaatttatc agccaagagg ccaaaaagca
     4921 atcgttgtca tggctggttc attcgagtct ctaccattgg ggaggagtga ggacggtctt
     4981 gatctattca caacattggc cacttcgggc agcgtggatg tggtattatg ggatgttgcg
     5041 gttggatacc gctcatacta tcgaaaggat gactcttaac tatggcgttg caaccgcgaa
     5101 cgggtgtgac agcccagccg gtcagccgac tgattgacga gctgtcgaag ctgccgggaa
     5161 ttggtcccaa gaccgcctcg cggctcgcat accacattct caagacgccg aaggatgacg
     5221 ccctcggtct ggccgaggcg atcctggagg tgaaggagcg catcacgctc tgttcggtct
     5281 gtttcaacac gaccgacatc gacccctgcg cgatttgcgc caacgagggt agggagaacc
     5341 gaattctcgt cgtcgaacaa ccgctcgacg tggtcgcgat cgagcgcacg catgagtaca
     5401 agggtcgcta ccacgtcctg catggcgccg tctcaccggt tgacggaatc ggaccggaca
     5461 agttgcgcat tcaggagctg gtcgaccgtg tccagcagcg tcttccggac gaagtcatcg
     5521 tgttcaccaa cctggatctc ccgggcgagg cgacggcaac ctatctgcat cggattttga
     5581 ccccgatggg tatccgtgtc acacggccgg cgagcggcct gccggtaggt ggggatctcg
     5641 agtacgccga tgaccagacg ctcggtcggg cgttgagcgg gaggcgagag ctgtagccgg
     5701 gctattgagc aaggtggccg tacaggctac tatagagaca atgattgaga cgccgcggtc
     5761 gacgatcgat cgccaccgtt ctgttatcac tcgctcgtcc cagcgggcgc ctttccacct
     5821 cctgtcaccc accggccggc gggagcccgg cgaaagccgc gccagcaccc cgcgctatgc
     5881 tcccggctac gatcccgacc tgacggcagg gcgtccagcg ctgtccgatc tcccggcgct
     5941 tgtcaccgcg ccccggttcc cgctcgatct cgcccgacga gatcactcgg cacagtggct
     6001 cgccgaccgc gccatccgcg agcggctcgg actctttcgg ggaaacgttc ggatgcgggt
     6061 ctcccgacga gccggctgtt tggccgcggc ggcggcgttt cgcgtgctgg ccccggattg
     6121 gcgctggcag ctggaagcgc tggttgcctg caatcccgag gacggcgccg cgttggatac
     6181 agcgctgcga tccggtgtca aggatgccgg cgaggctggc gcgagacgcg tgctggcccg
     6241 gttgcctgga gacccgacgg tagacagtgc gatgcggcgc gctggcttcg taccctttgt
     6301 caccgaattc gtctacgtgc tcgctgatga tcgacccggc aggccatccg gcgcgcccta
     6361 tgtccggcgg gtgcacccgg cggacgtctg gggtattcat cagctctatc tcgatgtcgt
     6421 gccgcgacag gtccagtacg ccgaggcggt gaccagccgg acatgggaac ggtcccggcc
     6481 actccggccg ggcacccgcc gctctagcgg ctgggtgatc gaggaccacg gccgggtcag
     6541 agggtacgcc cgggtctcga ccgaaatcga tcccggcgtg gtgcgtatcg atcttctcat
     6601 tgacccgctt cttcggacgc tggcgccgga tctgatctcg gcggttgtgt ccgaggccca
     6661 gcaacagcac ggcgtgccgt gtgtcgtcgt cattccgggg tacgcccagg agttgcatca
     6721 gcccgtacag gaggccgggt tcaggcacgt cggtgaccag acagcctggg tctgctacac
     6781 gaccgtgccc gcgcgatcgt acatcgtcgc tgtcgatctc ggtgctcccg cagctgccga
     6841 ggaccagcgc gcccgcgtgc ctggatttgg cggtgccggt atgagtgtga ccggaccggt
     6901 ggagtggggg aatcccgcct gacgctcgtc ggacccgacc acggcgcaaa tccccgctga
     6961 cgtcgctgga gaccgccgct ggcggtcggg acagaggaag gtacaacaac acttgagtac
     7021 tgctatccat acaccaccta cagtgcagca tgtcggggcg gagtatgcag taaccgataa
     7081 cctgaccgac atgctcgccg ttctgccctc gcgcatcgcc gcagcgatcc gcgagcacga
     7141 gaacatgcat gtcgacggtg ggctgatcga ggtcgtgatg gaccttggtc gtcccgctga
     7201 gatgcgctac cagcagggcg agatcacgct ggatgacttc gaggtcaccc gcgaggatct
     7261 caacgcggtg gcggaccgca tcgggcactt cggcgcggac aatcgtgccg ggatcgaacg
     7321 cacgctccac cgcatctcgg cgatccgcaa ccgcaccggg gacatcgttg gcctcacgtg
     7381 ccgtgtcggt cgcgctgtct acggcacgat cgcgatcatc accgatctgg tcgagtccgg
     7441 cgagagcgtc ctgttgctcg gccgcccggg cgtcggcaag accacgatgc tccgcgaaac
     7501 ggcgcgcgtg ctcgcggacg accttcggaa gcgcgtcgtg atcgtcgata cgagcaacga
     7561 gatcgccggc gacggggata tcccgcaccc ggccattggg cgtgctcgcc gcatgcaggt
     7621 cccgacaccc gaggcccagc acgccgtgat gatcgaggcg gtggagaacc acatgcccga
     7681 ggtcgtcgtg atcgacgaga tcggcaccga actcgaggcc ggcgccgctc gcacgattgc
     7741 cgaacgcggc gtccagttga tcggcacggc acatggtaac acgctcgaga acctcatgct
     7801 caaccccacg ctctccgacc tggtcggggg tatccagagc gtcacgcttt ccgacgagga
     7861 ggcgcgccgc cgcggcacgc agaagtcaat cctggagcgc aaggcgccgc cgaccttctc
     7921 ggcaatggtc gagatcgtcg accgcgacga ggtgatcgtc caccgggatg ttgccgcgac
     7981 ggtcgattcg atcctgcgtg gcgtcacacc accgagcgag aagcgtcggc ggcgcgagga
     8041 tggcacgatc gagtcggctt ccggtgatga cgcgcgccgc gatccaaccg gccggtcgtc
     8101 gtcgggatct ggcggtccgt cgctcacctc gatcgcggga aatgccagtg cgggtcagcg
     8161 tggcagccgc gacgtcgatt tcggatcttc gccccgggca ccgcgtgctc aggaggccgc
     8221 tgtccgccac aatctcaccg agaccgccaa tgggcagtcc gtccaggaga cgagcgagcc
     8281 ggcgccggtt gatccgcgca gcccgttcag tcggggtcgt ggcaccaggg ataagccgct
     8341 gcgcgtcttc ccgtacgggg tgagccgcaa ccgactgcat cagtcgatca cgcgcaaccg
     8401 ctatccggcg cagcttgtga aggatgcccg cgacgccgac ctgatcgtga cgctcaagaa
     8461 tgtcttcagg cgcaagcccg agttgctgcg gaacgcggag acgactggca cgccggttta
     8521 cgtcagtcgt tccaattcgg cggcccagct ggatgacgtg ttgcgcaatc tcttccccgg
     8581 catcgagatc gaggaggaga cggagctcgc gccatcgacg gtgccggccg ggttcaacgg
     8641 tggtaacgag caccgcatga tggatgcgtt gaacgaggcg gaatctgcga tcctgcgggt
     8701 gatcgatggc ggcccgcccg agtcgctgga accgcagtcg gtcggcattc gccgcaagca
     8761 gcacgagctc gccgagcggt acaatctcgc gtcccgtagt cgaggcaagg atcccttccg
     8821 ccacgtggag atctaccggc agggcgcgca gtagtgagtg ccggcacgtt tgtcgtgttc
     8881 gagggccccg agggggcagg gaaatcgacc cagatgcgcc gactcgccga tgtcctcgtg
     8941 cgggccggtc atgacgttgt cgccacccgc gagccaggcg ggacgcccgt tggtgacgcc
     9001 atccgccgcg tcctcctcga gtcggatggc tatgctatgc tccccgagac ggaggccttc
     9061 ctgctttctg ccgcccgtgt ccagcatgtt cacgaggtca ttcgcccggc gcttgaccgg
     9121 ggggcagttg tgctgtgcga tcgctatgcc gattcaacac tggcctacca aggtggtggg
     9181 cacggcatgg cggaaggtga cctccggtgc ctggagcgga tcgcgaccga tcgcctggta
     9241 cccgatctcc gcgttctgct cgatgtgccg gcggcagccg ggctggcgcg caggcaccgc
     9301 gataccacgt cggtgaatcg catcgatcgc gccagtctgg cctttcacga gcgggtcgct
     9361 gccgcgtacc ggttgctggc cgaacgagat cctgaagggt ggacggtgat cgacgcgacg
     9421 cagccaacgg atgctgtatt cacgatggtc tgtcaggcgg tgcagtcgtc actcggtttc
     9481 tcgctcgaac aggctggcgg ccagtgtcag gtgtgagcac gtcatgaaac tcgtcattgc
     9541 ggtggttcag gggaaggacg tcagcgaact gctgtcggcc ctgacggccg cgggctatcg
     9601 ggcgacgcag atcagcagct ctggtgggtt cctgcgggag agcaacgtca ccctgctgat
     9661 cggcgtggag gaccacgagg tcacatctgt gcaggcaatt atccaggaga actgtcactc
     9721 ccggacgcac ttcgtcaatc cgctcatgcc gatcgtcgag cccggcgaga gcctgatgcc
     9781 ggcgccggtg gaggtgccgg tgggcggggc gacggtattc gtgctcaggg ttgagcggtt
     9841 cgagcgtctc tagccc
//
LOCUS       DASYWL010000070         1995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_603548, whole genome shotgun sequence.
ACCESSION   DASYWL010000070 DASYWL010000000
VERSION     DASYWL010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603548"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VGR22_02480"
     CDS             <1..473
                     /locus_tag="VGR22_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127466.1"
                     /translation="PGQPAGEIHLDSVAAVRSTIETAQAGGATGAISVHYRDVEATFP
                     LQLGLILSGIGYSIAEDHDLTHSWLLGSLYTSITGMRRSFGGSGTRPEYRHHMAAIRA
                     MSAVASAHGVRNLKDEPLFQRAISAARQRVTAGEAPAITFRKVGKVGQVGSAPD"
     gene            complement(470..1660)
                     /locus_tag="VGR22_02485"
     CDS             complement(470..1660)
                     /locus_tag="VGR22_02485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEV2127467.1"
                     /translation="MKMHLPIEARLRNTESALRKAQERHRLALRAGGLGSWELDLSTM
                     TLETSEGCKVNFGLAPHDELPYSRLMEMMLPEDRPAMQASVKRAVETGEGYQAEYRIR
                     RPNGQITWIAAYGRPFFTADGTPEIMLGTTQDITDRKHAEAERERLLAEAHAARDEAE
                     RAVHMRDVFLTMTVHDLRTPVTAIRGRAQLLQRRLAKMQLTADDQQHIEEGLNWLEAS
                     TTQMEAILAELQDVALLEIGRPLELKLAPANLVALARSSIEQIRSQTDNHHICFDTPH
                     EQQFAAVDATRIGRVIDNLLSNAIKYSPEGGRVTISLGTDPEWAVLSVSDEGVGIPAG
                     EHELIFQRYRRGTNVTKRFEGIGIGLAGARQIVEQHKGSMHVESQEGQGSTFTVRLPI
                     GAGS"
     gene            complement(1788..>1995)
                     /locus_tag="VGR22_02490"
     CDS             complement(1788..>1995)
                     /locus_tag="VGR22_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEV2127468.1"
                     /translation="PITVGQRWVVERTIAWTNAHKKLVWCTERRAIVVAFWIAFSAVL
                     IIVGRLVREGWMRYRRDRRPRRRP"
BASE COUNT          336 a          671 c          613 g          375 t
ORIGIN      
        1 ttcccggaca gcccgccggc gagatccatc tcgacagcgt cgccgccgtg cgttcaacga
       61 tcgaaacggc acaggcgggc ggggccacgg gcgcgatcag cgtgcactac cgcgacgtcg
      121 aggcgacgtt cccgcttcag ctcgggctca tcctctcggg aatcggctac tcgatcgccg
      181 aggatcacga cctgacgcac tcgtggctgc tcggctcgct ttacacgagc atcacaggca
      241 tgcggcggtc gttcggcggg tcaggtaccc gaccggagta ccgtcaccat atggccgcga
      301 tccgagcgat gtcggcggtt gccagcgcgc acggtgtccg gaatctcaag gacgaaccct
      361 tgttccagcg ggcgatctcc gccgcccgcc agcgcgtcac cgccggtgaa gcccctgcca
      421 tcacgttccg caaggttggc aaggtcggcc aggttggttc cgccccagac taagagcctg
      481 cgccaatagg gagccgcacg gtaaaggtac tgccctgccc ttcctggctc tccacgtgca
      541 tgctgccctt gtgctgctca acgatctgcc tggctccagc cagcccgatc cctattccct
      601 caaagcgctt ggtcacattc gtccctcgcc ggtaccgctg gaaaatgagc tcgtgctcac
      661 ccgccggaat acccacaccc tcatcgctca cggacaggac cgcccactcc ggatccgtcc
      721 cgagtgagat cgtcacacgg ccaccctccg ggctgtactt gatggcgttg gacagcaggt
      781 tgtcgattac ccggccgatt cgggtggcgt cgacggcggc gaactgctgc tcgtggggcg
      841 tgtcgaaaca gatgtggtgg ttgtcggttt gcgacctgat ctgctcgatg gatgagcgcg
      901 ccagggccac caggttggca ggcgcaagtt tcagctccag cggtcggccg atctcgagca
      961 gcgcaacgtc ctgcaactca gccaggatgg cttccatctg ggtcgtgctg gcttccagcc
     1021 agttcagccc ctcttcgata tgctgctggt catcagcggt gagttgcatc ttcgccagtc
     1081 gtcgctgcag cagctgggca cggccgcgaa tggcggtgac gggagtgcgc aagtcgtgga
     1141 cggtcattgt gaggaagaca tcccgcatgt gcaccgctcg ttcggcctcg tctcgggcgg
     1201 cgtgtgcctc ggcgagcagc cgctctcgtt ctgcctcggc atgcttgcgg tcagtaatgt
     1261 cctgcgtggt gcccaacatg atctcaggcg taccatcggc ggtaaagaac ggccgaccgt
     1321 aggcagcaat ccaggtgatc tgcccattcg gtcgtcggat ccgatactcg gcttgatagc
     1381 cctcgccggt ttccaccgcc cgcttcacgg acgcctgcat ggcgggccgg tcctccggta
     1441 gcatcatctc catcagccgg ctgtagggta gctcgtcgtg gggggcgagg ccgaaattca
     1501 ccttgcaccc ttccgaggtc tccagcgtca tcgtgctcag gtccagctcc catgagccca
     1561 gcccaccagc ccggagagcg agccggtgtc gctcctgcgc cttacggagg gccgactccg
     1621 tgttacgcag acgcgcctcg atcggcagat gcatcttcat ggcctgacgt ccctttgacc
     1681 ttcggcgata cgcctagaat atgcctcatg ctacaataaa ggtaagcata aaacctgcgc
     1741 gtgactccca cgcgagacac ttagagcttg cgccaatagg ctcggcatca cggtcttcgg
     1801 cgcgggcgcc ggtcccggcg gtagcgcatc cagccttccc gcacaaggcg gcccacgatg
     1861 atgagcaccg ccgagaaggc gatccagaag gcgacgacaa tggcccggcg ctcggtgcac
     1921 cagacgagct tcttgtgggc gttcgtccag gcgatggtgc gctcgacgac ccaccgctga
     1981 cccacggtga tgggc
//
LOCUS       DASYWL010000071         4478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_622453, whole genome shotgun sequence.
ACCESSION   DASYWL010000071 DASYWL010000000
VERSION     DASYWL010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4478
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622453"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..374
                     /locus_tag="VGR22_02495"
     CDS             171..374
                     /locus_tag="VGR22_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127469.1"
                     /translation="MRRFSFLLAMILFMVPTPLVSAQVGTDSEQLDPGGHQLRWSGNW
                     YLDLQTSLAGPDYEGLVFEGNQM"
     gene            931..1230
                     /locus_tag="VGR22_02500"
     CDS             931..1230
                     /locus_tag="VGR22_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127470.1"
                     /translation="MQAFVSSLGVFDARQLDGGTLNNGTEWQLHRFTYQGIDLATFVT
                     VSLMSDGSYAVTSVTGNVDQMTLTLIEVQEQFSVNRSRSLLQGVDPILVTLTLLM"
     gene            1375..1992
                     /locus_tag="VGR22_02505"
     CDS             1375..1992
                     /locus_tag="VGR22_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127471.1"
                     /translation="MGRKLIAIVVLMASMIAHAASGIAQESAPPADSAEQSVTIAETE
                     VSWFGAWQFDEDASSDEIVTLKIVDEESGDLKLVTYAEMMDETVDDAGEALDVFATAF
                     LSTPGVSSVVEVATGELDDGAVWKVFAYDLGSVELATVFTVSERDDNTFVVSTLTAAI
                     PEFAEVVAEAQEQILLDGEPVFLGGIDREAFLPAPVATPQATPEG"
     gene            2234..3694
                     /locus_tag="VGR22_02510"
     CDS             2234..3694
                     /locus_tag="VGR22_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127472.1"
                     /translation="MRRERGFTAALAVPLALLLVLIAVLQPSATLAQTTATGQVTTQT
                     VGNSTITYSDVWVSEPSVPEGLLLIHQELRGTLFLYLEYDEPNLDATAVLSGGASEFF
                     SEFGDGNQEIVTIGGTTSIVDNVETRLSWQLYAVTQDSVPFGLLIAVDPTMLPGRAEV
                     SLLLAPAGSFDIAMQAVLDTIDVNGTGSPLSGMDPEDLAEDLEEFTQEAGPSGPAATE
                     PGATSTPSTTGGLTLPPLNPTTIAAAEETPAQPQETPAQPQETPAQTPGGLTLPPLNP
                     TATVQAAETPTQAPGGLTLPPLGGTSTAAPTGLLSGSATVAGMEIRYSDAWVLDTENS
                     TPGEILFLDAADEPLQFFGFLAQENAGLEAATALAVFNDVYFDNIGASTVTELAMETA
                     APGKAWSLHQAEIAGVPLMVLIYADAVSASGQIQFSLLAAEPAGMVDALTEAKASIQV
                     GGVPAFNELDPSMVASRLGGGPATTPLVVEAALRAA"
     gene            3885..>4478
                     /locus_tag="VGR22_02515"
     CDS             3885..>4478
                     /locus_tag="VGR22_02515"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV2127473.1"
                     /translation="MPIGRRTPATSIELHDDELVRLTAELVAIDSVNPDLVPGAAGES
                     AIADFCERWLAGQGLQTRRLESRPGRPSIVGIAPGTGGGRSLMLNGHYDTVSLGGYAG
                     DPLDPQVRGDRLYGRGAYDMKSGVATAMIAAARARRMRLSGDVIVACVADEEHGSLGT
                     EEVLMHYTADGGIVTEPSGLQAVIAHKGFVWFEVTILG"
BASE COUNT          810 a         1403 c         1460 g          805 t
ORIGIN      
        1 gtcttgcgta gagcctagtg tatcgagcgc gaacatgaaa tccgtcaaat gacgtatggg
       61 cctggatgga atcccgacct atgatgagtt gctgcacgac cgtgacctgt cacggagtgc
      121 gcatgggtcc catctggtaa caccacaatc acatatcccg aggagcaaac atgcgtcggt
      181 tctcatttct tctcgccatg atcctgttca tggtgccaac tccccttgtg tccgcgcagg
      241 tcggtacgga cagcgagcag ctcgatcccg gtgggcatca gttgcggtgg tccggcaact
      301 ggtatctcga tctgcagacc agcctggccg gccccgacta cgaaggcctg gtgttcgagg
      361 gcaaccagat gtaattcggc gtggtcgtgt acgccaacac cgtctcgggt gccgagctgc
      421 gagacatctt cgtcgatggc ttcgcctcgg agctgctgtc gtacggcacg gtcgatgtgg
      481 gcagccggaa cggtgtgcac tgggagctcg gtaacggtga tggcgtgacc ctttacgcgg
      541 ttttcattga tggcgatctg ggagcacagc cgctgggcta cttcgtggtc gccccgacga
      601 actcattcgc caactcggtc acggccatcc agcgtgactt cacccttgac ggcgttgccg
      661 tgctgcaggg agtggatggc ctcggtctcc agcaacgcgc acagccgggt ggcagccagg
      721 tctcggtcgt gccgccggtg caggactcga ccttcgtcta cgccacggag atcggctgga
      781 gcggcctgtg gacgctcgac accgtcaact cgtcgaacga ctacgccagc ttcatgcaga
      841 tgagcgaagc ggcgggcacg atgaagctcg tcgggtaccg agtactacga cctcaatgca
      901 gccacatccg gccaggcgct caacaattac atgcaggcct tcgtctcaag tctcggggtg
      961 ttcgatgcgc gccaactcga tggcggcacg ctcaacaacg gcaccgagtg gcagctccac
     1021 cggtttacct accagggtat cgacctggcg accttcgtga cggtctccct gatgtcggat
     1081 ggaagctacg cggtgacctc cgtcacgggg aacgtcgacc agatgacgct gacgctcatc
     1141 gaggtgcagg agcagttcag cgtgaaccgc agcaggtcgc tgctccaagg tgtcgatccg
     1201 atcctcgtta ccctcacgct gctcatgtag catcccgctg gctcagccag aagcgccttc
     1261 gcactcacga cgaccgcgag ggcgcttcca cggctgctcg tctcctgaca ccatcggtcc
     1321 aagaggctgc ctgatgtgcc tggggcattg gcttcgattc actggaggaa cacgatgggg
     1381 cgcaagctga tcgcgatcgt cgtgctgatg gcgagcatga ttgctcatgc tgcctccggc
     1441 atcgcccagg agagcgcccc gccggcggac tcggccgagc agtcagtcac gatcgcagag
     1501 accgaggtga gctggttcgg cgcctggcag ttcgacgagg atgcgtcgtc ggacgagatc
     1561 gtgacgctga agatcgtcga tgaggagtcg ggtgatctca agctcgtcac ctacgccgaa
     1621 atgatggatg agacggtcga tgatgcgggc gaggcactcg atgtcttcgc cacggcgttc
     1681 ctgagcaccc ctggcgtcag cagcgtagtg gaagtcgcca ccggcgaact ggacgacggg
     1741 gctgtctgga aggtctttgc ctatgatctc ggcagcgtcg agctcgccac cgtgttcacg
     1801 gtctccgagc gtgacgacaa caccttcgtc gtatcgacgc tgaccgcggc gatcccggag
     1861 ttcgcggagg tggtcgccga ggcgcaggag cagatcctgc tcgacggcga gccggtcttc
     1921 ctcgggggca tcgaccgcga agcgttcctt ccagcgccgg tcgctacgcc acaggcgacg
     1981 cccgaggggt gaccgaagtc ctgaaaaccg tgatccggct cacgtgaagg accgcccaga
     2041 cggatcgact cgcgatcatc atggcacgca gcgcgctggt gactggtcgc cgggatcgtg
     2101 ctcgactagc tcgatagcat catgtggccc acgccgtgtc cgatccgccg atagttatga
     2161 gagaatggct atgggtcatc atctcagtcc cgatcaacaa ttcacgaacc aatctgtgag
     2221 agaaggagtc cccatgaggc gagaacgtgg attcacggcc gcgctcgcag tgcccctggc
     2281 gttgctgctg gtgctgatcg ctgtgctgca accgtcagct accttggcac agacgaccgc
     2341 aaccgggcag gtgacgacgc aaacagttgg caactccacc atcacctact ccgacgtctg
     2401 ggtatcagag ccctcggttc ccgaaggcct gttactcatc catcaggagc tccgtggcac
     2461 gctctttctc tacctcgaat atgatgagcc aaatctcgat gccacagcgg tgctgtctgg
     2521 cggggcaagc gagttcttca gcgagttcgg cgatggcaat caggaaatcg tcacgatcgg
     2581 tgggacgacc tccattgtcg acaacgtcga gacccggttg agctggcagc tctatgccgt
     2641 gacgcaggat agcgtcccgt tcggcctgct catcgctgtg gaccccacca tgctgcccgg
     2701 acgagcagag gtatcgctgc tgctggcgcc ggcgggatcg ttcgacatcg cgatgcaggc
     2761 ggtgctggat accatcgacg tcaacggcac cggttccccg ctgagtggga tggacccgga
     2821 ggatctcgcg gaggacctcg aggagttcac ccaagaggcc ggtccctccg gcccggcggc
     2881 gacagagccg ggggcgacct caacaccgtc aaccaccggc ggcctgacct tgcccccgct
     2941 caacccaacg accatcgccg cggccgagga aaccccggcc cagccgcagg aaactccagc
     3001 tcagccgcag gagacaccgg cccagacacc tggcggcctc accttgccac cgttgaaccc
     3061 gacagctacc gtccaggcgg cggagacgcc aacccaggca ccgggcggac tcaccctgcc
     3121 gccactcgga ggcacgagca ctgcggcgcc aaccggtctg ttgagcggct cggcgactgt
     3181 ggccggcatg gagatccggt acagcgacgc ctgggtgctg gatacggaga acagcacgcc
     3241 gggcgaaatc ctctttctcg atgcggccga tgaaccgctg cagttcttcg ggttccttgc
     3301 ccaggagaat gcggggctcg aggcggcgac tgcgctggcc gtgttcaacg acgtctactt
     3361 cgacaacatc ggggcaagca ctgtcaccga gctggcgatg gagacggcgg caccgggcaa
     3421 ggcgtggtcg ctgcaccaag cggagatcgc tggcgtgcca ctgatggtgc tgatctacgc
     3481 ggacgccgtg tccgcgtccg gccagatcca gttcagcctg ctcgcggctg agccggccgg
     3541 catggtcgac gcgctgaccg aagcgaaggc atctatccag gttggcgggg tgcctgcctt
     3601 caatgaactc gatccttcga tggttgcgtc gcgtcttggt ggtggcccgg caacgacgcc
     3661 actggttgtg gaggccgcgc tccgcgccgc gtaagcactg tctgagacgg gcacattcgt
     3721 gatggctggc ccgctgcagg ccagcagagg cctggttccg accaacgggc ggctccgaaa
     3781 agagccgccc gttggtgtgt ccggagttgg tcgacactgc cgcaggactg ccccacaata
     3841 ccggccgggg agaggtccta tcgttcacag gaggatgcgc actcatgccc atcgggcgaa
     3901 ggacaccggc gacgagcatt gaattgcacg atgacgagct ggttcggctc acagctgagt
     3961 tggtcgcgat cgattcggtc aatcccgatc tcgtgcccgg cgccgccggg gagtcggcaa
     4021 tcgctgattt ctgcgagcga tggctggcgg gccaaggcct gcagacgcgg cggctggaat
     4081 cgcgtcccgg gcgtccctcc atcgtgggga tcgcgccggg caccgggggt ggccgctcgc
     4141 tgatgctcaa cgggcattat gatacggtct cgcttggtgg gtacgctgga gatcccctcg
     4201 acccgcaggt gcgcggcgat cgcctctacg gccgtggggc ctacgacatg aagagcgggg
     4261 tggcgactgc gatgatcgcc gcggcacggg cccggcgcat gcggctcagc ggcgatgtga
     4321 ttgtcgcctg tgtcgccgat gaggagcacg gcagcctcgg caccgaggag gtgctgatgc
     4381 actacaccgc cgacgggggg atcgtcaccg aaccaagtgg gctgcaggcg gtgatcgcgc
     4441 acaagggatt cgtctggttc gaggtcacca tcctgggg
//
LOCUS       DASYWL010000072        17582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_78251, whole genome shotgun sequence.
ACCESSION   DASYWL010000072 DASYWL010000000
VERSION     DASYWL010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 17582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17582
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78251"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VGR22_02520"
     CDS             <1..242
                     /locus_tag="VGR22_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127474.1"
                     /translation="NAVPKLRTVLWQFVGKPVSPELMADIVRVRLDERALRSYFEGLL
                     SCDEINALIARFNRLLNSGCHPILDPNRNIPYGWW"
     gene            complement(538..891)
                     /locus_tag="VGR22_02525"
     CDS             complement(538..891)
                     /locus_tag="VGR22_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127475.1"
                     /translation="MARESPSVTQGLAVANAASTPAMTHRRVLGSVIPVIGENLLQTG
                     VSVVDTMIVAALAATAVALTFPTCGFQSVYGGALRATGEARTPRWPTSSRRGWPWASS
                     GRGYGGPMPGLPLSG"
     gene            complement(1006..2421)
                     /locus_tag="VGR22_02530"
     CDS             complement(1006..2421)
                     /locus_tag="VGR22_02530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV2127476.1"
                     /translation="MTDEAHAGATAGIETTESPSEMTQGRVLRLALPIIGENLLQTTV
                     GVVDTLFVAALGAAALAGVGVALEVVFFAIAILSSIAIGGTVLVSQAIGARSTESANR
                     LARQTVLWGAILSVPLAVLAYSTAPGLVGLFHTEPEVADHAVTYLRIIGGTIVTLLMT
                     FVCGAVLRGAGDSQTPLRASLVANVVNVFLAYTLIFGKLGFPRLEVAGSAWSTTIGRA
                     VSAGILLWFLFSGRRAVSLAGRYGWRPQVRTARKLMTIGVPSAIEQMLMSAGFTAMIA
                     IVALIGTDALAAQQISFNALAMAFLPGFGFAIAATALVGQSLGAHDLDAAKTAARLSA
                     RWSLLWMTTGGTIYFIFSESILRIFTDNESVIGYGDGALKALAVSLPLWTFWTVYGGS
                     LRGLGDARTPMVASALSMWLAVGLAYVLVRWFDGSLTLVWATFLVTSPIAAVINTLMF
                     HRRLRKTRDEVAAGLPPPTPA"
     gene            2658..2978
                     /locus_tag="VGR22_02535"
     CDS             2658..2978
                     /locus_tag="VGR22_02535"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEV2127477.1"
                     /translation="MSNSFGPEDIKEFSALGPAQGLGCSIVASLLVFILGGVFLDQYL
                     DTAPLLTLIGVAIGLFAAGYQLYELTLIDRKDRPAGPIGRTLESKLEPRRRKQPTDRG
                     DGVQ"
     gene            3078..4073
                     /gene="atpB"
                     /locus_tag="VGR22_02540"
     CDS             3078..4073
                     /gene="atpB"
                     /locus_tag="VGR22_02540"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEV2127478.1"
                     /translation="MEVHVEIAAETLFEIGPVAVTNSMLTMFMVMAAILVVFSLIARK
                     ASMIPGRGQGVIELIIEFLLGLVEGTAGRRVGRRIFPLIAGIFIFILFANFSGLLPGV
                     GTIVYEHHEEVAHVEEAVDPDEGELADPSADEVTEAETEAEGAEHATGSVAVETDVDE
                     AEEEEAHGSAIPVLRPPTADLNMTLAMSLLTFIVVQVAGISAHGVVGRIKHMGDPPFI
                     FPIEVISEFSRIVSLAARLFGNVFAGEVLLAVMYSLANAIKIAIIPVVFPVVFIGLEV
                     LFGTIQALVFALLTLIYIALATAGHDEHGHIEEGQVDETVESSSNRNAAAPVSGD"
     gene            4249..4491
                     /gene="atpE"
                     /locus_tag="VGR22_02545"
     CDS             4249..4491
                     /gene="atpE"
                     /locus_tag="VGR22_02545"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEV2127479.1"
                     /translation="MFDGITEVEAVQYFASAFAIGIGAIGPALGIGMAVRGAMEAMGR
                     NPEAAGDIRTTMIIGAALAEAVAIYAFVIAIIILFV"
     gene            4622..5128
                     /gene="atpF"
                     /locus_tag="VGR22_02550"
     CDS             4622..5128
                     /gene="atpF"
                     /locus_tag="VGR22_02550"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEV2127480.1"
                     /translation="MLADLGINGWQLLIQMIAFIIFIALLWKFAVGPITHLLDERQGN
                     IRESMEAAERMQAELKETQARNEQVLQEARREAQNIVSTARTNGDQMIARAREEAAAQ
                     ADEYLERARGTLRQETDQARQQLRNEVADLAVLAAGRIVRRELDPSAQARLIEETLAE
                     AATESRTA"
     gene            5239..5769
                     /gene="atpH"
                     /locus_tag="VGR22_02555"
     CDS             5239..5769
                     /gene="atpH"
                     /locus_tag="VGR22_02555"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEV2127481.1"
                     /translation="MASGAAKRYVQAIVEIARENGSFDAWKGDLERFEAIARDETVLA
                     YLQNPSVQDARKKQAVDVVLTEAQPEARNLAHLLIERHRVDIIPEIVQGFDEALLAEQ
                     GIVLADVTTAEAIDPSMQEAVRERIGELVGKRVQLRMHTDPDIIGGLIARIGDEVIDG
                     SVQTRLRRMRARLKAS"
     gene            5886..7457
                     /gene="atpA"
                     /locus_tag="VGR22_02560"
     CDS             5886..7457
                     /gene="atpA"
                     /locus_tag="VGR22_02560"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEV2127482.1"
                     /translation="MAVRATEISDLLRQQIQGFSDDATITNVGQVISVGDGVATVHGL
                     TKAMQSELVEFPKTGTRGLVLNLEEDSVAVIVMGDYTNIEEGDEVRTTGAVASVPVGD
                     ALLGRVVNALGEPIDGKGPIHTEKTRDIERIAPGVIKRQDVDTGLQTGIKAIDAMTPI
                     GRGQRQLILGDRQTGKSTIAIDTIINQRDSDVKCIYVAIGLKQAQVAQMVKVLEDNGA
                     MDHTIVVAASAADPAPLQYIAPFAGTAMGEEFMENGGDALIVYDDLTKHAQAYRQVSL
                     LVRRPPGREAYPGDVFYLHSRLLERSARMHEDLGGGSLTSLPLIETQAGDISAYIPTN
                     VISITDGQIYLESDLYYAGIRPAINTGLSVSRVGGAAQIKAMSKVAGSLRLDMANYRS
                     LAAFAQFGTSDLDRSTRRQIERGQVMTEILKQPQYNPLPVEEEVAILWAGTNGYVDDV
                     PVDRVREFETQYLEFLRTSHSDLLESIVTEKRLTDEVVERLERVTKEFKDTNDFGEED
                     DATADASLEEATQTA"
     gene            7477..8343
                     /gene="atpG"
                     /locus_tag="VGR22_02565"
     CDS             7477..8343
                     /gene="atpG"
                     /locus_tag="VGR22_02565"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEV2127483.1"
                     /translation="MASPREIRRRIRSVRNMSQITRAMEMVAASKMRRAQDRVTASRP
                     YSERLREFIAGLSSQQMDAEELQRYPLLAERPLENAGLILLTPDRGLTGALNGNILRR
                     GAQFIREDVDVPTKVIAVGKKGRDFMARTNQDMIADFVGLGDAPSLDDIRPIAKVAMD
                     EFLSGGVDRVDMVFPRFINTLTQKPEAVQILPIEQQESQVEDVDYIFEPDAETVLEEV
                     LPRYVEVLLYQGILETVASFYSAQMVAMRNATDNANELADDLNLSYNKARQAQITNEV
                     AEIAAGANAFRA"
     gene            8421..9860
                     /gene="atpD"
                     /locus_tag="VGR22_02570"
     CDS             8421..9860
                     /gene="atpD"
                     /locus_tag="VGR22_02570"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEV2127484.1"
                     /translation="MAAPAVAQETKTAGATGTIVQITGVVVDVEFPADQLPEIYNALE
                     TELSDDTKLTLEVQTHLGNDRVRTVAMSTTDGLRRGAPVVDTGQAIAVPVGAATLGRI
                     FNVTGDPIDEMGPVDAEEYRPIHQDPPAFEDQATSVEVLETGSKVIDLIAPFTKGGKV
                     GVFGGAGVGKTVIITELIRNIAAEFGGYSVFCGVGERTREGTALWGELAESGVRDQTA
                     LIFGQMNEPPGARLRVSLTGLTMAEFFRDEGRDVLVFIDNIFRFVQAGSEVSALLGRM
                     PSAVGYQPTLGTEMGQLQERITSTRQGSITSVQAVYVPADDYTDPAPSSVFAHLDATL
                     ALERSIAERGIYPAVDPLASTSRILDPLIVGQEHYDVARDVQQVLQRYRDLQDIIAIL
                     GVEELSDEDKQTVNRARRIERFMSQPYFTAEQFTGTPGRFVSLADTVRSFREILDGKH
                     DDKPEAAFMYKGSIDEVVEAAEQMEAENR"
     gene            9923..10357
                     /locus_tag="VGR22_02575"
     CDS             9923..10357
                     /locus_tag="VGR22_02575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEV2127485.1"
                     /translation="MANLSVEIVTGERVVYEQDDVSMVVAPGAEGTLGILPSHAPLVS
                     LLQEGELRVRSGAGEESLAIYGGFLEVVNNKVLILSDSAERSGEIDLERAEAARARAE
                     ETLRNVRDTQDMAEAEAALRRASIRLRIARNRRQRGQERPAG"
     gene            10534..11880
                     /gene="murA"
                     /locus_tag="VGR22_02580"
     CDS             10534..11880
                     /gene="murA"
                     /locus_tag="VGR22_02580"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV2127486.1"
                     /translation="MGEVTLTSTTPATDERLLKIRGGRVLRGEVPISGAKNAALKAMA
                     ATLLTTEEVVLNNVPILADVLSMAKLLREFGAEVEVDRDRSRVRVRAEHIARTTAPPE
                     LFRPTRASVVAAGPMLARAGEVSFYMPGGDQIGRRPIDVHLRGFQRLGATVEHRSNQV
                     IATGGTLRGARIYMDYPSHTGTENLLMAATMAAGKTVIVNASVEPEVVWLGHLLNRMG
                     ARIEGLGSPIVTIEGVERLHGISDYVPPDRLEAGSFAIAAVMTGGEVVLRDIREPHML
                     PLTEKLMEMGAEVWTSNDMMLVRAGKTLKAVDIQTLPFPGFPTDLQAPFTALLTQANG
                     VSRVHERVYEDRLRYTAELQKMGADIRVERIGHNAGNGGELLAASAEVRGPTWLHGAR
                     VSAIDIRAAVCMVLAALVAEGETELDEVYHIDRGYEDFVAKLSAIGADLEDTAPQV"
     gene            11983..12366
                     /locus_tag="VGR22_02585"
     CDS             11983..12366
                     /locus_tag="VGR22_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2127487.1"
                     /translation="MSSRILIVEDQEDVAQLIEIVLKNEGYTVAVAPDGAQGLMLARD
                     WKPDLILMDIMLPGVDGGKLITRLRKEADTRDLPIVAMSASRTLRDRTPELEADALLA
                     KPFDVDALLVQVQFLLSRERGDDAS"
     gene            12363..13118
                     /locus_tag="VGR22_02590"
     CDS             12363..13118
                     /locus_tag="VGR22_02590"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEV2127488.1"
                     /translation="MTSGPSAAPSTTPDTAACDVLVVIPSYNERDNLPELIPQILALG
                     RGYCVLVVDDSSPDGTGQIADTLAAASNGRVRVLHREEKEGLGRAYLAGFQQALASDV
                     PLVAQMDADLSHRPQDLRTLVGAMLPGVDLVIGSRYVPGGATEGWPLPRRMISRAGGM
                     YARAVLGLPIHDLTGGFKVWRRDLLATIGLPEIVSDGYGFQIETTYRAVKRGAKVVQV
                     PIVFHDRVAGKSKLSRRVVFEAALRVWQFRFGR"
     gene            13245..14525
                     /locus_tag="VGR22_02595"
     CDS             13245..14525
                     /locus_tag="VGR22_02595"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEV2127489.1"
                     /translation="MVTSSRHEPEEMLDRREAQAIAVISQGLAAPSPRRRIRQSDLRD
                     VVERLGCVQLDTISVISRSHETVGWSRLGPYDLSLWDTLYEPAHAVTEYWAHAAAIVP
                     VADLRLHRPVMERFRRRAVQERDEATRALYNAVLERIREQGPLSSLDFEAPEGAAASA
                     WQWYGHKPERRALADLWSEGRLVVRRREAFRRIFDLIERVVPELWDGEVDEAEVRLAL
                     GRKALSALGLTTLPWFTDYFRTGGRPHLPRPIAQQVLATLVERGEAVPLAIAGIDEPA
                     WAAPEAMETLATLRKRRAWPTRTTLLSPFDNLVWNRQRDEQLWGFQYRLECYTPAPKR
                     IYGYYSLPILYRGALVGRLDPSFDRRAKLLTIKSFHWEEGVRPHDAMRRAVAGAIGEL
                     GGFLGGEPDRWVVNGHPPGTVPENVGRSWRGHDH"
     gene            complement(14592..15833)
                     /locus_tag="VGR22_02600"
     CDS             complement(14592..15833)
                     /locus_tag="VGR22_02600"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEV2127490.1"
                     /translation="MAANQSQRNAGTSTNQSNQGDVALLIDYENLQVSLKRHFEVTTP
                     KMSLIIQEAQDHGRLVLARAYAPWTSPDLSIDAENLYRQGIDLVYVPTRKNSADVRMA
                     VDAVESCQRAPNISTYVIVTGDGDLIHPLNYLRQQGRTVVVIGVDAAMSRLLSAAADS
                     VLLYERDLDPAVRQPAPSTRAQQRPSGQQEQQKTSLMPTSKEYPPAEEAFRMVQDALA
                     RRGNGEPVLFQELGHWLSQDHNLKPRVWYGVPFSTFLDAAEQAGHVQLTTSGGNSYAA
                     LPATIDTEEALLDDISEDDTSGTAGDSDDGSTVRLESLRPEERIALFNALSDLQESKR
                     NSYMSFRLLLKHLVSNSILPRLSEWQVRRLLNDLADRKPPILVRGQKTGKTPGGRSYT
                     FSTFTLTEDQKVLAQTERGEE"
     gene            15940..16917
                     /locus_tag="VGR22_02605"
     CDS             15940..16917
                     /locus_tag="VGR22_02605"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV2127491.1"
                     /translation="MSDLPTVVRERDPQIPPRVFDTHVHFPWGTGRHPSAVSDELVEL
                     AKKLNIVHMALLGSRREDYNDRVSQAIEHYPDLFIGMYGVELGEDTPEVIQDAHDRGF
                     RGLKIIHALKPYDDRSYWSVFEKAEELGIVTLFHTGVVGGGVDFRDTDPFSPEVVKRS
                     PFFEKRLRGQGFSSRHMDPILLDTLAFTFPRLRIIGAHMGVGQYDYACHIARWRRNVF
                     WDISGGELVRRHVFERRLIGQEISPMKILFASDCGMEKMGNEIADWAAVFDQMRLTIE
                     ERDRIFYGNAARLFALDEFAGVTNESCYDEIWNSYQLEDTGEISPADDA"
     gene            complement(17021..17275)
                     /locus_tag="VGR22_02610"
     CDS             complement(17021..17275)
                     /locus_tag="VGR22_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127492.1"
                     /translation="MPRIETIKASEAPAPPKKMSKATQEILDAINRLQKEEVLRLQPD
                     PGKSIRGLKTSVGRIASSNDLKIESWTDPAGDYLYARKAQ"
BASE COUNT         3150 a         5554 c         5882 g         2996 t
ORIGIN      
        1 tcaacgcggt gccgaagctc cggacggtcc tatggcagtt cgtcggcaag ccggtgtcgc
       61 ccgagttgat ggcagacatc gtccgtgtgc gcctcgacga gcgggcgctg cgatcttact
      121 tcgagggatt gctgagctgc gacgagatca atgcgctgat cgcccgcttc aaccgattgc
      181 tcaacagcgg ctgtcatccc atcctcgacc ccaaccgcaa cattccctat ggctggtggt
      241 gacgccagct tgaggagcac cgggtcccac agctcgactt cctcgacgtt cgcaccgtgc
      301 gagtccggga atccaacgga cagcccggca gctcggtagg ccccatggag gggcggaccc
      361 gtcggtcttc tttatctgca tctgccttgg aacctttgat cacagcggca tgggtgggac
      421 acggccggcc atgctacagc ggtgtctttg cctcgttcag ggcggcggcc cgatcagcca
      481 ggcggcgccg cacgatcccc acggtcatcg ccgtgattgg actggtgatg actatcgcta
      541 gccagataaa ggtaggcccg gcatcggccc accgtacccc cgcccagacg atgcccacgg
      601 ccagccacgt cgcgacgatg tcggccacct cggcgtgcga gcttcacccg tcgcccgcaa
      661 ggcacctccg tagacagact gaaacccgca cgtcggaaag gtgagggcga ccgccgtcgc
      721 agcgagcgcg gcgacgatca tcgtgtcgac tacgctcacg ccggtctgca gcaggttctc
      781 gccgatgacc gggatgaccg agccgagtac ccgccgatgg gtcatggccg gagttgaggc
      841 cgcgtttgcc acggcaagtc cctgcgtcac actgggcgac tcccgggcca tggggcagtt
      901 ccttgatgag ctcacgcctt gagcggcagg acaacggtgc aggacttggg agccccggag
      961 cgttcagagc gccggacgcg cgtcggagcg cagcatgcag gcgagtcagg ccggtgtggg
     1021 cggcggcagc ccagctgcga cctcatcgcg cgtcttccgg agccggcggt ggaacatcag
     1081 cgtgttgatc acagcagcga tcgggctggt gacgagaaac gtcgcccaga ccagggttag
     1141 cgatccgtca aaccaccgca cgagcacgta cgccagtccg actgcgagcc acatcgagag
     1201 agcgctggcc accattgggg tgcgtgcatc gcccagcccg cgcagggatc ccccgtagac
     1261 cgtccaaaag gtccagagcg gcaggctgac cgccagcgcc ttcagggcgc cgtcgccgta
     1321 accgatgacg ctttcgttgt cggtgaagat gcgcaggatc gactcgctga agatgaagta
     1381 gatggtaccg ccggttgtca tccagaggag cgaccagcgt gccgagagcc gagcggccgt
     1441 cttcgcggcg tctaggtcat gcgccccaag gctctggcca accagcgccg tggcggcgat
     1501 cgcgaacccg aatcccggca ggaatgccat cgcgagcgcg ttgaagctga tctgctgcgc
     1561 agcgagcgcg tcggtgccga tcagggcgac gatcgcgatc atggcggtga atccggccga
     1621 catcagcatc tgctcgatgg cggacgggac accgatcgtc atgagtttgc gtgcggttcg
     1681 cacctgcggc ctccagccgt atcgtcctgc caatgagacc gcccgacggc cggagaagag
     1741 gaaccagagt aggatacccg ccgagaccgc tcggccgatc gtggtgctcc acgcgcttcc
     1801 ggcaacttca agccgcggga agccgagctt cccgaaaatc agcgtgtagg cgaggaagac
     1861 attgaccacg ttggcgacga gtgacgcccg tagtggcgtc tggctgtcgc ccgcaccacg
     1921 caacaccgca ccgcagacga aggtcatcag caaggtgacg atcgtaccgc caatgatgcg
     1981 gagatacgtc acggcgtggt cggcgacctc cggctcggta tggaacagcc cgacgagtcc
     2041 gggggcggtg gagtaggcga ggaccgccag cggcactgag aggatggcgc cccagagcac
     2101 ggtttgccgg gcgagccggt tggcgctttc cgtgctgcgg gcgccgatcg cctgggagac
     2161 gagcactgtg ccgccgatgg caatggagct gagaatcgcg atcgcgaaga agacgacctc
     2221 cagcgccacg ccgacgcctg ccagcgcggc cgctccgagg gcagcgacga agagcgtatc
     2281 gaccacgccg accgtggtct gcagcaggtt ctcgccgatg atcggcaggg ccagccggag
     2341 cacgcgtcct tgcgtcatct ccgatggcga ctccgtggtt tcgatgcccg cggtagcgcc
     2401 ggcgtgggcc tcgtcggtca tggcgtccgt ctcatgaggg tacgggtatg gtatcaggcc
     2461 ggcacagtgc cgacgtgcga cacatcagga gagtccgaga acccgattgc cacggccgcg
     2521 cgacgggcat attgttggga agcgtacccg gcagtgtggc accatgctac gaagcaggtg
     2581 agtcgttggc tctcgttggt tgtttcgcat cgagattgtg ataatattcg caatcggaaa
     2641 agcgaggagg ggcgagggtg tccaactcgt ttggccccga ggacatcaag gagttcagcg
     2701 cgctgggacc agcgcaagga ctcgggtgct cgattgtggc atcgctcctg gtgtttatcc
     2761 tcggaggggt cttcctcgac cagtacctcg atacagcgcc gttgctgacg ctcatcggtg
     2821 tcgcaatcgg acttttcgcg gcggggtacc agctctacga gctgacactg atcgaccgga
     2881 aggatcgccc ggcaggcccg atcgggcgca cgctcgaatc gaaactggag ccgcgccgcc
     2941 gcaaacagcc caccgatagg ggcgacggcg tacaatgagg gcacaaccgc cgtcctgggc
     3001 ggctctcggt gtgccacggt gaagacggcc agagcgcggg tgccgcgccc ggaacggtga
     3061 ggagagacgc ctgatccatg gaagtccacg tagaaatcgc ggccgaaacc ctcttcgaga
     3121 taggcccggt tgccgtcacc aactcgatgc tcaccatgtt catggtgatg gcagccatcc
     3181 tggtcgtctt cagcctgatc gctcgcaagg catcaatgat tccaggtcgc ggtcaggggg
     3241 tgatcgagct gatcatcgag ttcctcctcg gccttgttga gggcaccgcc ggcagacgtg
     3301 tcggtcgccg catcttcccg ctcatcgccg gtatcttcat cttcattctc ttcgccaact
     3361 tctccggcct gctgccgggc gtcggtacca tcgtctatga gcatcatgag gaggtggcgc
     3421 acgtcgagga ggctgtcgat cccgacgagg gagagcttgc ggacccaagc gccgacgagg
     3481 tgaccgaagc ggaaacggag gcagaaggtg ccgagcatgc caccggttcc gtcgcagtgg
     3541 aaaccgatgt agacgaggct gaggaggagg aagcgcatgg ctccgccatt ccggttctgc
     3601 gtcctcccac cgccgacctg aacatgaccc tggcgatgtc gctgctgacg ttcatcgtcg
     3661 tgcaggtcgc cggcatctcc gcccatggcg tggtcggccg tatcaagcac atgggcgacc
     3721 cgcccttcat cttcccgatc gaagtgatct ccgagttctc gcgcatcgtt tcgctcgcgg
     3781 cccgtctctt cggcaacgtc tttgccggcg aggtgctgct ggcggtgatg tactcgctgg
     3841 cgaacgccat caagatcgcc atcattccgg tggtcttccc ggtggtgttc atcggcctcg
     3901 aggtgctctt cggaaccatc caggcgctgg tgttcgcgct gctgaccctg atctacatcg
     3961 ccctggcgac cgcgggccac gatgagcatg gccacatcga agagggccag gtcgacgaga
     4021 cggtcgaatc ctcttcgaac cggaacgcgg ccgcgcccgt gtccggcgac taacccacct
     4081 atgcgagtca ggccactccc cgggcagacc gggcgaagaa tgagtggcga gagctcggtg
     4141 agcaggtttc tttcgagaaa cagcaggcag ctccagtgct gcctgcaccg gaacgaatga
     4201 cggggctgtc gaaaaaggac gacaggcgcc cacaggagga gacgtcacgt gtttgacgga
     4261 attacagagg tcgaggcagt acagtatttc gcgtccgcgt tcgccatcgg catcggggcc
     4321 atcggtccgg cgctcggtat cggtatggcc gtgcggggcg cgatggaggc catgggtcgc
     4381 aacccggagg ccgccggcga catccgcacg acgatgatca tcggtgcggc gctggccgag
     4441 gccgtcgcga tctacgcctt cgttatcgcg atcatcatcc tcttcgtcta ggcccgtacc
     4501 ctgtcgatgg tggcgccgtc ggggtgccac catcgcggtt gcgagctccc gcccggaccc
     4561 gggttgggcg aagaggcgat cggggctccc agcgcggggg catcacgcga aggagtggcg
     4621 aatgctggcg gatctcggga tcaatggatg gcaactgctg atccagatga tcgcgttcat
     4681 tatcttcatt gccctcctgt ggaagttcgc ggtcgggccg atcacccatc tgctcgatga
     4741 gcggcagggc aatattcgcg agagtatgga agcggccgag cgaatgcagg ccgagctgaa
     4801 ggaaacgcag gcccggaacg agcaggtcct gcaggaggcg cgccgcgagg cgcagaatat
     4861 cgtttccacc gcccggacca acggcgacca gatgatcgcg agggcccgcg aggaggccgc
     4921 cgcccaggcg gatgagtacc tcgagcgcgc ccgcggcacg ctgcgccagg agaccgatca
     4981 ggcacgccag cagttgcgca acgaggttgc ggacctggcg gtgctcgcag ctggccgcat
     5041 cgtccggagg gagctcgatc cttccgcgca ggcgcgactg atcgaggaaa ccctcgccga
     5101 ggcggcaacc gagtcgcgga cggcgtagca catacctggc acgcggaagg cggtatggta
     5161 gagacggctc ggtccgtctg ccacgggcct ccggcgtact gacgcgcagg cacgcaacga
     5221 acagggagcg aacgagcgat ggcaagcgga gcagcgaagc gctacgttca ggcgatcgtc
     5281 gagatcgctc gggagaacgg aagcttcgac gcctggaagg gggacctcga gcgattcgag
     5341 gccatcgccc gggacgagac ggtgctcgcc tatctccaga acccgagcgt ccaggacgcc
     5401 aggaagaagc aggcggtcga cgtcgtcctg acggaggcgc agcccgaagc ccgcaacctc
     5461 gcgcacctgt tgatcgagcg ccatcgggtc gacatcatcc ccgaaatcgt gcagggcttc
     5521 gacgaagccc tcctcgccga gcaggggatt gtccttgccg acgtgacgac cgccgaggcg
     5581 atcgatccgt cgatgcagga ggcggtgcgc gagcgcatcg gcgaactcgt cggcaagcgg
     5641 gtccagctcc ggatgcacac ggatccggac atcatcggcg gcctgatcgc ccgaatcggc
     5701 gatgaggtga tcgacggcag cgtccagacc cggctccgcc ggatgcgggc gcggctgaag
     5761 gcttcctgac cccggtcagg aacgaggcga cccggtccga tggggccggt gatggttggt
     5821 acacataggg gcgcagacag gaaccgcgcg aagcggcgct catgtcgcgc agaactggag
     5881 cgatcatggc ggtacgcgcg accgagatca gcgatctgct caggcagcag atccaaggat
     5941 tttccgacga cgccaccatc accaatgtcg gtcaggtgat cagtgttggt gacggcgtcg
     6001 caaccgtgca tggcctcacc aaggcgatgc agagcgagtt ggtggagttc cccaagacgg
     6061 gcacccgcgg gctggtcctc aacctcgagg aggattccgt ggcggtcatc gtcatgggcg
     6121 actacaccaa tatcgaagag ggcgacgagg tccgcaccac cggcgcggtc gcctcggtgc
     6181 cggtcggcga cgcgctgctg ggccgagtgg tgaacgcgct gggcgagccg atcgacggca
     6241 agggcccgat ccacaccgag aagacgcgcg acatcgagcg catcgcgccg ggcgtgatca
     6301 agcgccagga cgtggatact ggcctgcaga ccggcatcaa ggcgatcgac gccatgacgc
     6361 cgatcggccg cggccagcgc cagctgatcc tgggtgaccg gcagaccggc aaaagcacga
     6421 tcgcgatcga cacgatcatc aaccagcgcg acagcgacgt gaagtgcatc tacgtcgcga
     6481 tcggcctgaa gcaggcacag gttgcgcaga tggtgaaggt gctcgaggac aacggcgcga
     6541 tggatcacac catcgtggtc gccgcctcgg ccgccgaccc ggcgccgctc cagtacatcg
     6601 cgccgttcgc gggcaccgcg atgggcgagg agttcatgga gaacggcggc gacgccctga
     6661 ttgtctacga cgacctcacc aagcacgccc aggcctaccg ccaggtgtcg ctgctggtgc
     6721 gccgcccgcc gggacgcgaa gcctacccgg gcgatgtctt ctacctgcac tcccgcctgc
     6781 tggagcgctc ggcccggatg cacgaggacc tcggcggcgg ctcgctgacc tcgctgccgc
     6841 tcatcgagac gcaggccggt gacatctccg cgtacatccc gaccaacgtg atctcgatca
     6901 cggacggcca gatctacctc gagtcggacc tctactacgc gggtatccgc ccggccatca
     6961 acaccggcct gtcggtttcc cgtgtcggcg gcgccgcgca gatcaaggca atgagcaagg
     7021 tcgccggcag cctccgcctc gacatggcga actaccgctc gctggcggcc ttcgcgcagt
     7081 tcggcacgag cgacctcgac cgttccacgc gccggcagat cgagcgcggc caggtgatga
     7141 ccgagatcct gaagcagccg cagtacaacc cgctgccggt cgaggaggag gttgcgattc
     7201 tctgggcggg caccaacggc tacgtcgatg atgtgccggt ggaccgcgtg cgcgagttcg
     7261 agacgcagta cctcgagttc ctgcgcacct cgcacagcga cctgctggag tccatcgtca
     7321 ccgagaagcg gctgaccgac gaggtcgtcg agcggctgga gcgggtgacg aaggagttca
     7381 aggacacgaa cgacttcggg gaggaggacg acgccacggc ggatgccagc ctcgaggaag
     7441 cgacacagac cgcctaggca ggaaggataa ggcgcagtgg caagcccaag agaaattcgg
     7501 cgccggatcc gcagtgtccg aaacatgtcg cagatcactc gcgccatgga gatggtggcg
     7561 gcgtcgaaga tgcgacgcgc gcaggaccgc gtgaccgcgt cccgcccata cagcgagcgg
     7621 cttcgcgagt tcatcgccgg tctgtcgtcg cagcaaatgg acgccgagga gttgcagcgg
     7681 tacccactat tggcagaacg gcccttggag aacgccggcc tgattctgct gacgccggat
     7741 cgggggctca ccggcgcgtt gaatggcaac attctccggc ggggtgcgca gttcatccgg
     7801 gaggatgtcg atgtgccgac caaggtgatc gcggtcggca agaagggccg tgacttcatg
     7861 gcccggacca accaggacat gatcgcggac tttgttgggc tcggggatgc tccctcgctc
     7921 gatgacatcc ggccgatcgc caaggtcgcc atggacgagt tcctgtccgg cggtgtcgac
     7981 cgggtcgaca tggtgtttcc aaggttcatc aacacgctga cgcagaagcc tgaagcggtg
     8041 cagatcctgc cgatcgagca gcaggagagc caggtcgagg acgtcgatta catcttcgag
     8101 cctgatgctg aaacggtatt ggaagaggtt ctccctcggt acgtcgaggt gctcctctat
     8161 caagggattc tcgaaacggt cgcgtcgttc tactcggcgc agatggttgc gatgcgcaat
     8221 gcgacggaca acgcgaacga gcttgcggac gacctcaacc tttcctacaa caaggcgcgg
     8281 caggcgcaga tcaccaacga ggttgccgaa atcgctgctg gagccaacgc gttccgcgca
     8341 tgacgcgaca aagcaaccaa cggcacggtg atcgcagatt gattcgcgac cggtctcaga
     8401 agagaaacga ggtaatgtac atggctgcac cagcagttgc tcaggaaacc aagacggcag
     8461 gtgcgacggg gacgatcgtc cagatcaccg gtgttgtggt cgacgtcgag ttcccggcgg
     8521 accagcttcc cgagatctac aacgcgctcg agaccgagct gagcgatgac acgaagctga
     8581 cgctcgaggt gcagacccac ctcgggaacg accgcgtgcg cactgtcgcg atgagcacca
     8641 ccgatggcct gcgccgtggg gcgccggtcg tcgacaccgg ccaggcgatc gcggtgccgg
     8701 tcggtgcggc aacgctcggc cgtatcttca atgtcaccgg cgacccgatc gacgagatgg
     8761 gcccggtcga tgcggaggag taccgcccga tccaccagga tccgcctgcc ttcgaggatc
     8821 aggcgacctc cgtcgaggtg ctggagaccg gctcgaaggt catcgacctg atcgccccgt
     8881 tcaccaaggg cggtaaggtc ggcgtcttcg gcggcgccgg cgtcggcaag accgtcatca
     8941 tcaccgagct catccgcaac atcgcggccg agttcggtgg ctactcggtc ttctgcggcg
     9001 tgggcgagcg cacgcgcgag ggcacggcgc tctggggcga gctggcggaa tccggcgtgc
     9061 gcgaccagac ggcgctgatc ttcggccaga tgaacgagcc gccgggcgcc cgcctgcgcg
     9121 tgtcgctgac cggcctgacg atggcggagt tcttccgcga cgaaggccgc gacgtgctgg
     9181 tgttcatcga caacatcttc cgcttcgtcc aggcgggctc cgaggtctcg gcgctgctgg
     9241 gccgcatgcc gagcgccgtg ggctaccagc cgacgctggg taccgagatg gggcagctcc
     9301 aggagcgcat cacttcgacc cggcagggct cgatcacctc ggtgcaggcc gtgtacgtgc
     9361 cggccgacga ctacaccgac ccggcgccgt catccgtgtt cgcccacctc gacgcgaccc
     9421 tcgcgctgga gcggtcgatc gccgagcgtg gtatctatcc agccgtcgac ccgctggcgt
     9481 cgacgagccg catcctcgac cccctcatcg tcgggcagga gcactacgac gtcgctcgtg
     9541 acgtccagca ggtccttcag cgctatcgcg acctgcagga catcatcgcc atcctcggtg
     9601 tcgaggagct gagcgacgag gacaagcaga cggtgaaccg ggcgcgccgc atcgagcgct
     9661 tcatgtccca gccgtacttc accgccgagc agttcaccgg cacgccgggt cgcttcgtgt
     9721 cgctcgccga caccgtacgt tccttccggg agatcctcga cggcaagcac gacgacaagc
     9781 cggaagcggc cttcatgtac aagggcagca tcgacgaagt cgtcgaagcc gccgagcaga
     9841 tggaagccga gaatcgctag cgaagccggg cccgcagttg ctacaagcga ctccgggccg
     9901 gcacagctga aggaggctgg acatggcgaa tctctcggtc gaaatcgtta ccggtgagcg
     9961 cgttgtctat gagcaggacg atgtctccat ggtcgtcgcg cccggtgcgg agggcacgtt
    10021 gggcatcctg ccaagccatg cgccgctcgt gagcttgctg caagaaggtg agctacgggt
    10081 acggtctgga gcaggcgagg agtccctcgc catctacggt gggttcctcg aagtcgtcaa
    10141 caacaaagtc ctgattcttt cggattccgc agagcggtct ggcgagatcg atctcgagcg
    10201 agccgaagcg gcccgtgccc gggccgaaga gacactgcgc aacgtccggg acacgcagga
    10261 tatggccgaa gccgaagcgg cactgcgtcg cgcttcaatt cgcctgcgca ttgcgcgaaa
    10321 ccggcgtcag cgtggtcaag aacgtccggc cggataggcc agttcgcggc tgaacctgca
    10381 caggcgaccg gagcgagcca tcgggccgct ccggtttcgc gtgcgtggct cgtgccctgc
    10441 gatggcagga cgtatacttg tatgtacacc gggagcatcg ctcccggccc ccacttgcga
    10501 tcacatccac gcccccgttt ccaaggagct ctgatgggcg aagtcaccct cacatccacc
    10561 accccagcaa ctgacgagcg cctgctcaag atccggggcg gccgcgtcct gcgcggtgag
    10621 gtgccgatca gcggcgccaa gaacgcggcc ctcaaggcga tggccgcgac actcctcacc
    10681 accgaggagg tggtgctgaa caacgtgccg atcctggccg acgtgctctc gatggcgaag
    10741 ctcctgcgcg agttcggggc cgaggtcgag gtcgaccgcg atcggagccg ggtgcgcgta
    10801 cgggcggagc acatcgcccg caccacagcg ccgccggagc tcttccggcc gacgcgcgca
    10861 tcggtcgtcg cggcggggcc gatgctggcg cgggctggcg aggtgtcgtt ctacatgccg
    10921 ggcggcgacc agatcggccg ccgacccatt gacgtgcatc tgcggggttt ccagcggctc
    10981 ggcgcgacgg tcgagcatcg aagcaatcag gtgattgcca ccggcggcac cctccgcggc
    11041 gcccggatct acatggacta tccgagccat acgggcaccg agaacctgct gatggccgcc
    11101 acgatggctg ccggcaagac agtgatcgtg aacgcgtccg ttgagccgga ggtcgtctgg
    11161 ctgggtcatc tgctcaaccg catgggcgct cgcatcgagg gcctcggctc cccgatcgtc
    11221 acgattgaag gtgtcgagcg gctgcacggc atctccgact atgtgccgcc cgatcggctc
    11281 gaggcagggt ccttcgcgat cgccgcggtg atgaccggcg gcgaggtggt cctgcgcgat
    11341 atccgtgaac cgcatatgct gccgctgacg gagaagctga tggagatggg tgccgaggtc
    11401 tggacgagca acgacatgat gcttgtacgc gccggcaaga ctctcaaggc ggtcgatatc
    11461 cagaccctgc cgttccccgg cttcccgacc gacctccagg cgccgttcac cgcgctcctc
    11521 acgcaggcga acggtgtgtc ccgggtgcat gagcgggttt acgaggaccg gctgcgctac
    11581 accgccgagc tccaaaagat gggtgctgac atccgcgttg agcgtatcgg gcacaacgct
    11641 ggcaatggcg gcgagctcct cgcggcctcc gcggaggtgc ggggcccaac ctggcttcat
    11701 ggagcacgtg tctcggcaat cgatatccgc gcggccgtct gcatggtgct ggcagcgttg
    11761 gtggccgagg gcgagacgga gctcgacgag gtctaccaca tcgatcgggg atacgaggac
    11821 ttcgtggcga agctctcggc gatcggcgcc gatcttgagg acaccgcgcc gcaggtgtag
    11881 cgcctcgatg acaggccgcc ttgtacgtac gagatcgacg tgggatgata ggtgcagtat
    11941 cacgctggac cgctggtcgc cagtgacaga ggacgtgtgt cagtgtcatc ccgcattctc
    12001 atcgtcgaag atcaggaaga cgtcgcgcaa ctgatcgaga tcgtcctgaa aaatgaggga
    12061 tacaccgttg ctgtcgcgcc tgacggggct cagggcctca tgctggcgcg ggattggaag
    12121 ccggacctca tcctcatgga tatcatgctg cccggggttg acggcgggaa gctgatcacg
    12181 cgtctccgca aggaggctga cacgcgagac ttgcccatcg tggcgatgtc cgcaagccgc
    12241 acactgcgcg accgcacgcc cgagctcgag gccgacgccc tgctggcgaa gccattcgac
    12301 gtcgatgcct tgctggtgca ggtgcagttc ctcctttcgc gcgagcgggg ggacgacgcc
    12361 tcgtgacgtc ggggccatcg gctgcccctt ccacaacccc ggacacagcc gcctgtgacg
    12421 tgctggtggt cattcccagc tacaacgaac gggacaacct cccggagctc atcccgcaga
    12481 ttcttgcgct gggccgaggc tactgcgtgc tggtcgtcga cgacagctcg ccggacggca
    12541 ccggtcagat cgccgacacg cttgccgcgg cgtcaaatgg ccgcgtccgt gttctccatc
    12601 gggaggaaaa agaggggctc ggaagggcct acctcgccgg gtttcagcag gcgctggcat
    12661 ccgatgttcc acttgtggcc cagatggacg ctgatctctc ccaccgtccg caggatctcc
    12721 gcacgctggt cggagcaatg ctacccggcg tcgacctcgt gattggcagc cgatatgtac
    12781 cgggcggcgc gacggaggga tggccgctgc cacgccgaat gatcagccgt gcgggtggga
    12841 tgtatgcccg ggcagtgttg ggtctaccga tccacgacct taccggtggc ttcaaggtct
    12901 ggcggcgcga tctgctggca accatcggcc tgcccgagat cgtctccgac ggctacggct
    12961 tccagatcga gacgacctac cgggccgtca agcggggtgc aaaagtcgtg caggtcccga
    13021 tcgtgttcca cgatcgcgtg gccggcaagt cgaagctctc ccgtcgggtc gtgttcgaag
    13081 ccgccctgcg cgtctggcag ttccgctttg gccgctgatc cacgtaccgt ccgctgcgca
    13141 acagttgacc tgacgcggta gaacggcgtc gtcggatgtc cgccttcatg tcgtacatca
    13201 caccaagaat atgctcctaa tcactgaaaa gtctgggaga gtccatggtc acatcgtcga
    13261 gacacgagcc agaggagatg ctcgaccggc gggaggcaca ggcgatcgcg gtgatctcgc
    13321 agggcctggc cgcaccgtca ccccgtcgtc gcattcggca gagtgacctt cgggacgttg
    13381 tcgagcggct cgggtgcgtg cagctcgaca ccatcagtgt catttctcga tcccacgaaa
    13441 ccgtcgggtg gagccggctc ggcccctacg atctgtcgct ctgggatacg ctgtacgagc
    13501 cggcacatgc cgttaccgag tattgggcgc acgccgcggc gatcgtgccg gtcgcggatt
    13561 tgcggcttca tcgccccgtg atggagcgct tccggagacg tgccgtgcag gagcgcgatg
    13621 aggctacccg agcgctgtac aacgccgtgc tggagcgcat ccgggagcag gggccgttgt
    13681 cctcgctcga ctttgaggcg ccggagggcg cggccgcctc ggcgtggcag tggtacggcc
    13741 acaagcccga gcggcgcgcc ctcgcggact tgtggtcgga ggggcggctg gtcgtgcggc
    13801 ggcgggaggc gtttcggcgc atcttcgacc tcatcgagcg ggtcgttccc gagctctggg
    13861 acggcgaggt cgacgaggcc gaggtgcggt tggcgctcgg tcgcaaggcg ctgagcgcgc
    13921 tcggcctgac tacgctgcca tggttcactg actatttccg caccgggggc cgaccgcacc
    13981 tcccccggcc aatcgcccag caggtgctgg caacgctggt agagcgcgga gaggcggtgc
    14041 cgcttgccat tgcagggatc gacgagccgg cgtgggcggc tcctgaggcg atggagacgc
    14101 tggcgaccct ccggaagcga cgggcctggc cgacccgaac cactcttctc tcgccttttg
    14161 acaaccttgt ctggaatcgc cagagagacg aacagctgtg gggcttccag tatcggctgg
    14221 agtgctatac accggctccg aaacgcatct acggatacta ctccctgccc atcctctacc
    14281 gcggagctct tgtgggacgg ctggatcctt cgtttgatcg cagggcgaag ctcctgacca
    14341 tcaagagctt ccactgggag gagggtgttc ggccacatga tgcgatgcgt agggcggtcg
    14401 ccggcgcaat tggggagctt ggcgggtttc tcggcggtga gcccgaccgg tgggttgtca
    14461 atgggcaccc ccctggaacc gtcccggaaa acgtgggccg ttcctggcgt ggccacgatc
    14521 actaactgtc tcggtccgcc ggtgcagcgc ctccggtgac cgatggcggc ctcttgaaac
    14581 aacgatgtgg cctactcctc gccgcgttcc gtttgcgcca gaaccttctg atcttccgtc
    14641 agcgtgaacg tcgagaaggt gtaggagcgt ccgccgggcg tcttgccggt cttttgccct
    14701 cggacgagga tagggggctt gcggtccgcg agatcgttga gcagccgccg cacctgccac
    14761 tcgctgaggc gcggcaggat gctgttggac acgaggtgct tgaggagcag ccggaacgac
    14821 atgtagctgt tccgtttgct ctcctgcagg tcgctgagcg cgttgaagag ggcgatccgc
    14881 tcctcgggac gaagtgactc taggcggacc gtcgatccgt cgtccgaatc cccggcagta
    14941 cccgacgtgt cgtcttccga gatgtcgtcg agcaatgcct cctccgtgtc gatcgtggcg
    15001 ggcagggccg cgtagctgtt gccgccggag gtggtcaact gcacgtgccc tgcctgctct
    15061 gcggcatcga gaaaggtgct gaagggaacg ccgtaccaga cccgcggttt gaggttgtgg
    15121 tcctggctga gccagtggcc gagctcctga aagagcaccg gctcgccgtt gccccggcgc
    15181 gcgagtgcat cctgcaccat ccgaaaggcc tcctcggcgg gcgggtactc cttcgatgtc
    15241 ggcatgagcg acgttttctg ttgctcctgc tggccggagg ggcgctgctg ggcacgcgtg
    15301 gatggtgccg gctggcgtac agcgggatcg aggtctcgtt cgtagagcag gacggagtcc
    15361 gccgctgccg agagcaggcg gctcatcgcg gcatccacgc cgatcacaac cacggtccgg
    15421 ccctgctgac gcaggtagtt gagcggatgg atcaggtcgc catcgccggt gacgatcaca
    15481 tacgtggaga tattcggggc ccgctggcac gactccacgg cgtccaccgc catccggaca
    15541 tccgcgctgt tcttgcgtgt cggcacgtag accaggtcga taccctgccg atagagattt
    15601 tcagcgtcga tgctgaggtc cggcgaggtc cacggcgcgt aggcgcgcgc cagcacgagg
    15661 cgaccgtgat cctgcgcctc ctggatgatg agactcatct tcggcgtggt cacctcgaag
    15721 tggcgtttca ggctcacctg gaggttctcg taatcgatca gcagggcgac gtcgccctgg
    15781 ttgctctggt tcgttgatgt gcccgcgttg cgctgcgact ggtttgcggc cacgttatgc
    15841 tcctcccgcg acggtgccga gttcacgtac gagagtacag tacgatacca tcggcccgcg
    15901 atgccgcgtg gtatcaccga ggagagggag aaagaacgca tgagcgatct gccgacggtc
    15961 gtgcgcgagc gcgacccgca gatacccccg cgggtcttcg acacccacgt gcacttccca
    16021 tggggaacgg gccgccaccc atccgcggtc agcgacgagc tggtcgagtt ggccaaaaag
    16081 ctgaacatcg tccacatggc cctgctgggc agtcggcggg aggactacaa cgatcgagtg
    16141 agccaggcga tcgagcacta cccggacctg ttcatcggca tgtatggcgt cgagctcggc
    16201 gaggatacac ctgaggtgat tcaggacgca catgaccgcg gcttccgggg gctgaagatc
    16261 atccacgcgc tgaagccata cgacgaccgc tcctactggt cggtgttcga gaaggcggaa
    16321 gagcttggga tcgtgacgct tttccacacc ggcgtcgtcg gtggcggcgt cgactttcgg
    16381 gacaccgacc cattcagccc ggaagtcgtc aagcgatctc cgttcttcga gaagcggctg
    16441 cgtgggcaag ggttctcgtc gaggcacatg gatccgatcc tgctcgacac gctggcgttc
    16501 accttcccgc ggctgaggat catcggcgca cacatgggtg tcggtcagta tgactacgcc
    16561 tgccacatcg cgcgctggcg gcgcaatgtc ttctgggaca tctccggcgg cgaactggtg
    16621 cgacggcacg tcttcgagcg ccggttgatc gggcaggaga tatcgccgat gaaaatcctc
    16681 ttcgcgtcgg actgcggcat ggagaagatg ggcaacgaga tcgccgactg ggcggcggtg
    16741 ttcgaccaga tgcgcctgac gattgaggag cgcgaccgca tcttctacgg caatgcggcg
    16801 cgcctctttg ccctggacga gttcgccggc gtcacaaacg agtcgtgtta cgacgagatc
    16861 tggaattcgt atcagctaga ggatactggg gagatcagcc cggccgatga cgcatagggc
    16921 gggtcttcaa tcgccgtact tgtactgggt gatcctctga agacaaacgc ccactgccat
    16981 cggcgggtgc gttcaatcag gctgatctgt cgctcctggt ctactgcgcc ttccgggcgt
    17041 agaggtagtc ccccgccggg tcggtccagg actcgatctt gaggtcgttg ctcgaggcaa
    17101 tgcgccccac cgatgtcttc agcccacgta tactcttgcc cgggtccggc tgaaggcgca
    17161 ggacttcctc cttctgcaat cgattgatgg cgtcgaggat ctcctgcgtc gccttcgaca
    17221 tcttcttcgg cggtgccggc gcttccgacg ccttaatggt ttcgattcgt ggcatagctg
    17281 ccccctttcg gtggggagta tagcctgagc taaatgcacc gcatgctggc acggaaatgc
    17341 ctgctgcctc actgccggat gggattggcc taccacctaa ctagattctc aagccttcgg
    17401 tcagcttggt gtccgcatcc gtggtgccca tagtgcggtg taccacccgg cgccgatcac
    17461 gcctcccacc catggctgac cgatcgcctt agaagtcatc caagaactag ctatcgtggc
    17521 gccgggaggg ttccacgatg gcgaccgcag tgccgacgga cctgacagac gaacagtggg
    17581 cg
//
LOCUS       DASYWL010000073         7730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_434728, whole genome shotgun sequence.
ACCESSION   DASYWL010000073 DASYWL010000000
VERSION     DASYWL010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7730
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434728"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VGR22_02615"
     CDS             complement(<1..336)
                     /locus_tag="VGR22_02615"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV2127493.1"
                     /translation="MPNIPSLSTISRRRAITIGAGAVIAAMTGTGSALGRQSGGSEYT
                     FVVAGLDYREGFDEHNSDVLMVSRVNLDLGTVRTVSIPRDLYVEIPGYGFDKITRAFH
                     HGYYSNGESW"
     gene            685..1074
                     /locus_tag="VGR22_02620"
     CDS             685..1074
                     /locus_tag="VGR22_02620"
                     /inference="COORDINATES: protein motif:HMM:NF025903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HEV2127494.1"
                     /translation="MSQVRIYGHRASLAPRRQAWSDCIHGCLVETLGLPDSKRFHRFF
                     PMDPADFIHPADRTEHYTIIEISMFAGRTEETKRRLIRLLLEWIETELGISVQDAEIT
                     IFESPAASWGIRGRCGDELALDYDVRV"
     gene            1235..1669
                     /locus_tag="VGR22_02625"
     CDS             1235..1669
                     /locus_tag="VGR22_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127495.1"
                     /translation="MTYPDPRVQQATAERFIPVRLHLTEHRQWTRQFQVFWTPTILIG
                     DRSGKIRYTSVNYLPPDEFLDILDIGEALVAMRWREYDTSIERLLAVEERRPIGPLTP
                     EATYWLGITEYFRQGRSSAASNAVWSRLVERFPDSIWAKRVP"
     gene            complement(1675..2199)
                     /locus_tag="VGR22_02630"
     CDS             complement(1675..2199)
                     /locus_tag="VGR22_02630"
                     /inference="COORDINATES: protein motif:HMM:NF019632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEV2127496.1"
                     /translation="MPDTDRYRDKTIEELLAEIDAAWGRLMETMRAEPEERYGAAQDA
                     ASWTPLDHMAHITAWERSVLFPLMGRPRHEGLGVTDEEFSMGFDPLNELIRRQTAGQG
                     YKDVMVSALREHQALVNMIDASSPEELWQPSRELCPDQREQHRELPFVVLLMSDTSEH
                     YDEHRGYIEKILAG"
     gene            complement(2385..2888)
                     /locus_tag="VGR22_02635"
     CDS             complement(2385..2888)
                     /locus_tag="VGR22_02635"
                     /inference="COORDINATES: protein motif:HMM:NF019632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEV2127497.1"
                     /translation="MAGQQESRRSVDEALGEIEVAWDRLMGAMTSWLEGDYASIHDAA
                     GWTPIDHLAHVSAWERSRIGWLQGHPRHKGLGVSFNTFAQDYDALNEAVRQQTAGQSY
                     QQVLADARTTHERMLAAIHAFDPSVVDRGGITSEEAARLGDELKENLTDHYEEHRADV
                     EEMLGSN"
     gene            complement(2944..3246)
                     /locus_tag="VGR22_02640"
     CDS             complement(2944..3246)
                     /locus_tag="VGR22_02640"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEV2127498.1"
                     /translation="MSIHPSALLAIILMAVVTYATRAGGYWIMGRVALSPRVAAMLVY
                     LPGAVLASLVVPVVLEEGIEGMIALGATALAMWWRGNLLIAMLTGVGRIWLVRLAL"
     gene            complement(3559..3924)
                     /locus_tag="VGR22_02645"
     CDS             complement(3559..3924)
                     /locus_tag="VGR22_02645"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEV2127499.1"
                     /translation="MGIPVAVSVAAFGVVFGALARQVGLTLLEVILMNTLVFAGAAQI
                     AALDLWVYPLPILAIVATTLLINLRLLLLGAALRPWLGRYPDRKVYPWLHIMADEGWA
                     LAMNRYARGERDAGFLMVC"
     gene            4142..4678
                     /locus_tag="VGR22_02650"
     CDS             4142..4678
                     /locus_tag="VGR22_02650"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEV2127500.1"
                     /translation="MTDSSREALGKRLKQYVRDIPDFPHKGILFRDITTLLADAAAFR
                     DAIDGMGHGYENIDIVVIIESRGFILGTPIAYLLSAGVVPVRKPGRLPAPTLEQAYSL
                     EYGAHALEIHQDAIKPGQRVLIVDDLLATGGTVAATIRLVELLGGEVAGISVLAELSD
                     LKGREHIGDYQISSLIVY"
     gene            complement(4889..6016)
                     /locus_tag="VGR22_02655"
     CDS             complement(4889..6016)
                     /locus_tag="VGR22_02655"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEV2127501.1"
                     /translation="MTENERPPLIPVFDGHNDTLLSLLTTGRDFFKESKTGHLDWPRA
                     KRGGLVGGFFAVFVPDPKSLEKQKVAVEDVLGGDPDDLVLVNENGPTLAYAQQFAMAE
                     IAALLRIETGDGTPYKRKRRLVTREGWDTVTPVITARDLKEQIEGGEFSAILHFEGAE
                     MIDTNFNALETFYETGLRSLGIVWSRSNAFGHGVPFGFPSSPDTGPGLTAAGKALVKA
                     CNQLGIMVDVSHLNEAGFWDVVDTTSKPIVATHSNAHAICPSSRNLTDKQLAAIRDSD
                     GIVGVNFNVSFLRPDGGRERDSPLHMMADHVDYLVDKVGLERVALGSDFDGALMPNDL
                     SDASKVPNLMAVLRQRGYDDAALKMIGYQNWVRVLTATWAV"
     gene            6164..6958
                     /locus_tag="VGR22_02660"
     CDS             6164..6958
                     /locus_tag="VGR22_02660"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HEV2127502.1"
                     /translation="MSETAASRAAGILSDKTCIVMGVQNRWSIAWQIAEAMANAGASV
                     AITYVDERAKRNAEQLAETVPGMKTFECDVTKDDSISNLATELKNEYGMIDTLVHSIA
                     YAPADELKNPFLQTSREGFRMAQDISVYSLIVVARELVPIMSEHGSIVTMTYLGSTRA
                     FPKYNVMGVAKAALEATVRYLALDLGEKGIRVNAISAGPIKTASARGIPGFSDMANMA
                     AERSPLGKVFSPQHVAGMAVFLASDASAGVTGDVIFVDGGYHAIGM"
     gene            7342..>7730
                     /locus_tag="VGR22_02665"
     CDS             7342..>7730
                     /locus_tag="VGR22_02665"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127503.1"
                     /translation="MKITGITATPIAFADPPLINFAGLHQPYALRTIVEVETDEGISG
                     FGEVSGGEKLQHAFRMAIPHLIGMDAFNRQAIRQKLFGSPEGALEKEHQIMPGMKDQR
                     RRELVFSAVEVALLDIAGKALGLPVHAL"
BASE COUNT         1432 a         2599 c         2419 g         1280 t
ORIGIN      
        1 ccacgactca ccgttgctgt agtacccgtg gtggaaggcc ctggtgatct tgtcgaaccc
       61 gtagcccggg atctcgacgt agaggtcgcg cgggatcgac acggtgcgaa cggtaccgag
      121 atcgaggttg acgcgcgaca ccatcagcac gtcgctgttg tgctcgtcga agccctcgcg
      181 atagtccagc ccggcgacca cgaaggtgta ttcgctgccg ccgctctgac ggcccagtgc
      241 cgagccagtg ccggtcatcg cggcgatcac cgcaccggcc ccaatcgtga tggcccggcg
      301 acgggagatg gtcgagagcg aggggatgtt gggcatgatc caattgctcc tcggaattgc
      361 ctggtgcatc gtggcgagcg gacgctcgcg ggctgaaaag gtcccaccgc cgcagtgacc
      421 agcggtgaca gctgagcgct gatacggaca gtatgtacga tcatggtatc gcaacgtcgg
      481 tcttcagccc gcaaatggct tcccggcgga ccagcacact gatggcaagt cgccttccag
      541 ccaagttcag acgacccgat gtggagccaa caccagccgc gaccagatgc aagatccagg
      601 ttgtcatcgg ccccatcgtt gcgatggcgt aggattgcac cgggacacta cggcagaatc
      661 agaaagctca cagaggtcaa cagcatgtcg caagtacgca tctacgggca tcgggcgtcg
      721 ctggcgccac gccggcaggc atggtcggat tgcatccacg gctgcctcgt cgaaacgctc
      781 ggcctccccg actccaaacg attccaccgt ttcttcccga tggatcccgc cgacttcatc
      841 caccccgcgg atcgcaccga gcactacacc atcatcgaga tcagcatgtt cgccgggcgc
      901 accgaggaga cgaaacgccg cctgatccgc ctgctcttag agtggatcga gacggagctt
      961 ggcatctctg tgcaggatgc cgagatcacc atcttcgagt cacccgccgc cagttggggc
     1021 attcggggcc gctgtggcga cgaacttgcg ctcgattacg atgtgcgcgt ctgaatcggc
     1081 gcgacacgga cgaacgccga caaaaggggc aaggatgagc atcgactggc tccgggactg
     1141 ggatgaggcc gtgaaggccg cgcgggcagc gcgaaagccg atcatgctgg acgtctatca
     1201 ggacaactga ggcggctgcg acaagctgga tgccgtgacg tatccagacc cccgtgtgca
     1261 gcaggccacc gccgagcgct tcattccagt caggctccac ctgaccgagc atcgccagtg
     1321 gacccggcag ttccaggtct tctggacccc aacgatcctc attggcgacc gcagcggcaa
     1381 gatccgctac acctcggtca actacctgcc gcccgacgag tttcttgaca tcctcgatat
     1441 cggcgaggcg ctggtcgcca tgcgctggcg ggagtacgac acctcgatcg agcggctgct
     1501 cgccgtcgag gagcgccgcc cgatcggccc gctcaccccg gaggcgacct actggctcgg
     1561 cattaccgag tacttccggc aaggtcgcag ctcggcggcg tcgaacgccg tctggtcccg
     1621 cctggtcgag cgcttccccg actcgatctg ggccaagcgg gtaccgtgac gtaatcagcc
     1681 cgcaagaatc ttctcgatgt acccgcgatg ctcgtcgtag tgctccgatg tgtcgctcat
     1741 gagcagcacg acgaagggca gctcgcggtg ctgctcgcgc tggtccgggc agagctcccg
     1801 cgatggctgc cacagctcct ctggactgga agcatcgatc atgttgacga gcgcctggtg
     1861 ctcgcgcagc gcgctcacca tgacgtcctt gtacccctgc cctgccgtct gccggcggat
     1921 gagctcattg agcgggtcaa agcccatcga gaactcctcg tccgtgacgc cgagtccctc
     1981 gtggcgcggc cggcccatca gcgggaagag cacggagcgc tcccatgcgg tgatgtgcgc
     2041 catgtgatcg agcggcgtcc agctagccgc atcctgcgcc gcaccgtagc gttcctcggg
     2101 ctctgcacgc atggtctcca tcagccgacc ccaggccgcg tcgatctccg cgagcagctc
     2161 ctcgatcgtc ttgtcccgat agcgatcagt atccggcatg gtcgaatccc tcctcgaaaa
     2221 tcaaacctcg gtaaccgtgg ccgttgtccc gagccggttg tgtcggctca tgtgcggaat
     2281 ctcgaccgcg atcagccacg acaccggaac tccgataccg agttcacaag agatgcctcg
     2341 gcacgtcacg caccgcaccg gctcggcttg acaggtgtcc gctgtcaatt cgagccgagc
     2401 atctcctcga catcggcacg gtgctcctca tagtggtcgg tcaggttctc cttcagctcg
     2461 tcaccgagcc gtgcggcctc ctcggaggtg ataccgcccc gatcgaccac cgaggggtca
     2521 aaagcgtgaa tcgccgcgag catccgctcg tgggtggtgc gtgcatccgc caggacctgt
     2581 tggtagctct gacccgccgt ctgctggcga acagcctcgt tgagcgcgtc atagtcttgc
     2641 gcgaacgtgt tgaagctcac gccgagcccc ttgtgccgcg ggtgaccctg cagccagccg
     2701 atccgcgagc gttcccaagc gctgacgtgg gcgagatgat caatcggagt ccatccggcg
     2761 gcatcgtgga tggaggcata gtcaccttcg agccatgaag tcattgcccc catcaggcga
     2821 tcccaggcaa cctcgatctc ccccagggcc tcgtccaccg agcgcctgct ctcctgctga
     2881 ccggccatcg tgccacatcc tttccgtaac ttacacccaa acactcggtg tagcgtccat
     2941 ccatcacaac gccagccgca ccagccagat ccttccgacg cccgtaagca tcgcgatgag
     3001 cagattgccg cgccaccaca tcgctagcgc ggtcgcgccg agcgcgatca tcccctcgat
     3061 gccctcctcc agcacgaccg ggaccaccag cgaggccagc actgcgcccg gaaggtagac
     3121 gagcatcgcg gcgacgcgcg gcgagagggc cactcgcccc atgatccaat acccaccggc
     3181 cctggtggcg taggtcacga cggccatcag gatgattgcg agcagcgcgc tcgggtggat
     3241 actcatgacg ctccctcgcg cgtgtccggc atgtgcacct tcgtcggatc gaccagcagc
     3301 gccgccgcca gagacacccc gccagcgcac cgatcacgat ataccacgtg cctggcagcc
     3361 attggtacgc cagccaggtg ctgacgccgg cgacggtcca cggcacgagg tcgaatgtgc
     3421 tgcgccagct gccgaaaagc attgccccga agattgccgc gaatgcgaag tcgagcccga
     3481 tcgccgcggg atcgccgatc tggccgccga acaggaagcc tatcgccgtt gccggcaccc
     3541 agccgaccgg caccatggtc agcacaccat cagaaacccg gcatcgcgct cgccgcgcgc
     3601 gtagcggttc atcgccagcg cccacccctc atcggccatg atgtggagcc acgggtagac
     3661 cttgcgatcc ggatagcggc caagccacgg gcgaagcgca gccccgagca gcaacaggcg
     3721 caagttgatg agcagcgtcg tcgcgacaat ggcgaggatc ggcaacgggt agacccagag
     3781 atcgagcgca gcaatctgcg cagccccggc gaagacgagc gtgttcatga ggatcacttc
     3841 gagcagggtc aacccgacct gccgcgccag cgcgccgaag acgaccccga aggccgccac
     3901 gctgaccgcg acgggtatcc ccaccaccag tccacgccgg tacccggccc tcgtccaatg
     3961 atgggcgtgg gtcgggaccg ctgtgatcga tccggcatgc tgatcgatgg tggggtcgac
     4021 gaggcgctag gtcatcgcac ggccattatc ggtcacaccg ccgctggggc acaaaatggg
     4081 tacactcagc tgggagtgcc gagcggggct ccatcgttcc agcataggat gggccaagac
     4141 catgaccgac tcgagccgcg aggcgcttgg caagcgcctc aagcagtatg tccgggatat
     4201 tcccgacttc ccgcacaagg gcatcctgtt ccgcgacatc accacactgc tggccgacgc
     4261 cgcggccttc cgcgacgcga tcgacggcat gggtcatggg tacgagaaca tcgacatcgt
     4321 cgtgatcatc gagagccgcg gctttatcct cggcacgccg atcgcttacc tgctgagcgc
     4381 aggggtggtg ccggtacgca agccgggccg cctgcccgcc ccgactctgg agcaggccta
     4441 ctcgctggag tacggagccc acgcgctgga gattcaccag gacgccatca aaccgggcca
     4501 gcgggtcctg atcgtcgacg atctgctcgc gaccggcggc acggtcgctg cgacgatcag
     4561 gctggtcgag ctgcttggcg gcgaggtcgc cggaatcagc gtgctggccg agctgagcga
     4621 tctgaagggc cgcgagcaca tcggcgacta tcagatcagc agcctgatcg tctattaggc
     4681 aaccctgaaa gattccgatg gacaggcggc gaccccggcc acccgtaagg gcagacctgg
     4741 agcctgccct gagcgagggc caacggcccg agtcgaacgg gtccgcccgc gctcgaatcg
     4801 gcacaagcgc ctttgctgag catagtgaag tttcgctccc acgtccggca agcggccgta
     4861 ggtggtcagc aacagtgata aggccgtgtc agaccgccca ggttgccgtg aggacccgaa
     4921 cccagttctg gtagccgatc atcttcagcg ccgcgtcgtc atagccacgc tgccgcagca
     4981 ccgccatcag gttcggcacc ttgctcgcgt cggagaggtc gttcggcatc agtgcgccgt
     5041 cgaagtccga gcccagcgca acgcgctcca gcccgacctt gtcgaccagg tagtcgacgt
     5101 ggtcggccat catgtgcagc ggcgagtcac gctctcgccc gccgtccggc cggaggaagg
     5161 acacgttgaa gttgacgccg acgatgccgt ccgaatcgcg gatcgccgcc agctgtttgt
     5221 ctgtgagatt gcgcgacgac gggcagatcg cgtgggcgtt ggagtgggtc gcgacgatcg
     5281 gcttgctagt ggtatcgacc acgtcccaga agcccgcctc gttgaggtgc gacacgtcca
     5341 ccatgatgcc gagctggttg cacgccttga ccagcgcctt gccggccgcg gtcagccccg
     5401 gcccggtatc cggcgacgac gggaagccga acggcacgcc gtgcccgaag gcgttggatc
     5461 tcgaccagac gatgcccagt gaccgaagcc cggtctcgta gaaggtctcc agcgcgttga
     5521 agttggtgtc gatcatttcg gcgccctcga agtgcaggat cgccgagaac tcgcccccct
     5581 cgatctgctc cttgaggtcg cgcgccgtga tcacgggcgt cacggtgtcc caaccctcac
     5641 gggtcaccag gcgacgcttg cgcttgtagg gcgtaccgtc accggtctcg atgcggagca
     5701 gcgcggcaat ctccgccatg gcgaactgct gggcgtaagc cagcgtcggg ccgttctcgt
     5761 tgacgagcac gagatcgtcg gggtcgccgc cgaggacatc ctccaccgcc accttttgct
     5821 tctcgaggct cttcgggtcc ggcacgaaga cggcgaagaa tccgccgacc agcccgcccc
     5881 gcttggcgcg tggccagtcg aggtgccccg tcttgctctc cttgaagaag tcccggcccg
     5941 tggtcagcag gctcagcagc gtgtcgttgt gaccgtcgaa caccgggatc aacgggggcc
     6001 gttcgttctc cgtcatgaat gcttcctcgg gttgcggtaa ccacctgata gaatgtggcg
     6061 cacggactcg gcggcatgcg aagccccgag caacagatac gcaccaagat accagcgtcc
     6121 ggctcggctg tgaccgattg gtgctggcgt gggaggattc gacgtgtccg aaacagcagc
     6181 ttcgcgggcc gcgggcattc tgagtgacaa gacgtgcatc gtgatgggcg tgcagaaccg
     6241 gtggagcatt gcctggcaga tcgccgaggc gatggccaac gccggcgcca gcgtcgccat
     6301 cacctacgtc gatgagcgcg cgaagcggaa cgccgagcag ctggccgaga cggtgccggg
     6361 catgaagacc ttcgagtgtg acgtgacgaa ggatgactcg atctccaatc tggcgactga
     6421 gctcaagaac gagtacggca tgatcgacac gctggtgcac agcatcgcct acgcgccggc
     6481 cgacgagctc aagaacccct tcctgcagac gtcgcgggag ggcttccgga tggcgcaaga
     6541 catcagcgtc tactcgctga tcgtggtcgc ccgcgagctg gtgccaatca tgagcgaaca
     6601 cggctcgatc gtgacgatga cctacctcgg ctcgacccgc gccttcccga agtacaacgt
     6661 gatgggtgtc gccaaggcgg cgctggaagc gacggtccgc tacctcgcgc tcgacctcgg
     6721 cgaaaagggc atccgcgtca acgccatctc cgccggtccg atcaagacgg cctcggcgcg
     6781 cggcatcccg ggcttcagcg acatggccaa catggcagcc gaacgctcgc cactgggcaa
     6841 ggtgttcagt ccgcagcatg ttgcaggcat ggccgtcttt cttgccagcg atgcatcggc
     6901 cggcgttacc ggcgacgtga tcttcgtcga cggcgggtac cacgccatcg ggatgtaggc
     6961 gggtcccgac aatacgcaac cgaacgccga cgccggagat gaatatcccc ggcgtcgttg
     7021 cgtgcacggg ggcaagacgc gtgtcacgaa cggcctcacc cctgtcatcc tgatcggagg
     7081 cccgaagggc cggagaagga tctcttgcgt gatggctatc aacgcccgag cggaggcgcc
     7141 actgtcacaa gagatccttc gacgcacatt cgcttcgctc agggaaggtt ctgctgcgcg
     7201 gaaggcgccg tgccggctca ggatgacaac cactaatggt ggcagctccg tgagcaagac
     7261 accgaagggc ccttctcgac gaagtcgcgc cggtctgctt tgatgctcca gacgagccgc
     7321 cagcaccgag gagcctcatc cgtgaagatc accggcatca cggccacccc gatcgccttc
     7381 gccgacccgc ccctcatcaa cttcgccggg ctgcaccagc cctatgcgct gcgcaccatt
     7441 gtcgaggtcg agaccgatga gggcatctcc ggcttcggcg aggtctccgg cggcgagaag
     7501 ctgcagcatg ccttccggat ggcgatcccg catctgatcg gcatggatgc gttcaaccgg
     7561 caggccattc gccagaagct ctttggctcg cccgaaggcg cgctcgagaa ggagcaccag
     7621 atcatgccgg gcatgaaaga ccagcgccgc cgcgagctcg tcttcagcgc ggtcgaggtc
     7681 gccctgctcg acatcgcggg caaggcgctc ggcctgccgg tccacgcgct
//
LOCUS       DASYWL010000074        24264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_172043, whole genome shotgun sequence.
ACCESSION   DASYWL010000074 DASYWL010000000
VERSION     DASYWL010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 24264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24264
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172043"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VGR22_02670"
     CDS             <1..345
                     /locus_tag="VGR22_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127504.1"
                     /translation="LRTDGPSVPWTPLEIRAERRCTSAATAVPFVVQAGPSVLMIEQS
                     HDGWVLAELRFDGEECLFRDIRVSSYAWPREAFGVLLSRLASSDLDAETVDQLSTDFA
                     EWVGIGFARIPQ"
     gene            complement(444..989)
                     /locus_tag="VGR22_02675"
     CDS             complement(444..989)
                     /locus_tag="VGR22_02675"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEV2127505.1"
                     /translation="MSTNTRLAGYRERRNAFFREHPNSPLTEEQKAEFEGLRYFPENP
                     DLNLTLEIDESGEGIGETIQVGTLSGEVKDYIRVGRIHFQSEGQPATLSVFKDTERGR
                     YFMPFRDATAGNESYSVGRYIDPKARPDGRLVVDFNMAYNPYCAYNTGWACPIPPSEN
                     ITNVRIPAGEMDPGLLPHDDE"
     gene            complement(1112..1711)
                     /locus_tag="VGR22_02680"
     CDS             complement(1112..1711)
                     /locus_tag="VGR22_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEV2127506.1"
                     /translation="MSHVDPKQIEGDASSDHVAEDTEAIHETAEAVFEEFQGELVSAF
                     IEIPRGSRNKYEYDEESGVWHLDRVLYSSVHYPTDYGWVPDTLAEDGDHLDILVLVQE
                     PTFPGCMIEARPLGGLDMSDEAGRDFKILAVPEGDPRFSHYQALEDVGPHWLREIETF
                     FQTYKLLEDKETEVLGWHGREHALKVLRECRDRYTHAKA"
     gene            complement(1708..2259)
                     /locus_tag="VGR22_02685"
     CDS             complement(1708..2259)
                     /locus_tag="VGR22_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127507.1"
                     /translation="MAFRVKKRGKLTYYYEGDRPVLSALVTEEQPDGDLRIHFAGLTG
                     GYSAKTVLGLEELVTMDPHREIPLVFRSWEKWLRDAGLADSLREVDFVEMHAFGCQPK
                     SPNPLVDPGGYAAEQQRLRTAYAEAYAGYFAEHLPDNGVPCRFTVHLVDVPDQAASYE
                     FYSVGLLQRSQGNRAEKTERNQA"
     gene            complement(2311..3138)
                     /locus_tag="VGR22_02690"
     CDS             complement(2311..3138)
                     /locus_tag="VGR22_02690"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEV2127508.1"
                     /translation="MPTLSVITDEISADLDRALTVCNELAISAVELRMIADQNVVELD
                     GAAVAGVKRTLERHGCSVVAIASPFLKCDYWGEDEMAHQREMGVLRRSITIAHALGAP
                     IVRAFSFWRAPDPSTLHQPALAALDEATEICRDSGVLLGFENEHACTVATGDEAAWFL
                     DRIADDTIGMIWDPGNEAVVGSVPFPEGYERVRSRIHHVHLKDARAIAPDPTFVVMGE
                     GAIDYIGQFRALADAGYDGALSLETHVASAPEEKEATSRASIAGIRALAEQAGLRLA"
     gene            complement(3237..3464)
                     /locus_tag="VGR22_02695"
                     /pseudo
     CDS             complement(3237..3464)
                     /locus_tag="VGR22_02695"
                     /inference="COORDINATES: protein motif:HMM:NF017174.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
     gene            complement(3769..5280)
                     /gene="dnaA"
                     /locus_tag="VGR22_02700"
     CDS             complement(3769..5280)
                     /gene="dnaA"
                     /locus_tag="VGR22_02700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEV2127509.1"
                     /translation="MIELATRAAAGDEGLAVEDQRGPVSGGPSPPDDSIAAAQLWQTV
                     LTELRHELNRNAYDNFFRHTALQSVDDDQVIVSAPNAFTANTLQTRYASPVERAIHGL
                     TGQRLRVEFIVRPTAGSERPRPQPPRAGQRRRNGERQKEQPQESPGLGTKQLELARPG
                     LHGLNPRLTYDTYVVGSSNRFAHAASMAVADQPGGKFNPFFVYGGVGLGKTHLLHAIG
                     HRAAENNPDLNIVYVSSETFTNDLINGIRAQKMEDFRSRYRTIDILMIDDIQFIAGKE
                     STQEEFFHTFNALYQNGKQVVISSDRPPKSISALEDRLRSRFEGGLIADVQLPDYEMR
                     TAILRTKADELGVSLPDDLIEYVAHRDQTNIRELEGALNKILMMAQLYNRKLDIQLAM
                     EALTDASIAQRRTTTTAADVLRAVCAEFGMEEKDLLGRARKREIVRPRHVAMYLLREE
                     TESSLVEIGRTLGGRDHTTVLHGVEKIEKDLESDPQLRARIIAIQEALLTGKS"
     gene            5463..6749
                     /gene="mnmA"
                     /locus_tag="VGR22_02705"
     CDS             5463..6749
                     /gene="mnmA"
                     /locus_tag="VGR22_02705"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEV2127510.1"
                     /translation="MVTCSLNQPVALAPDTSIDTEPLLCEERIGPYDGAGKTAVVAMS
                     GGVDSAVTALVMRERGYRVVGMNLRLFSPDDEDHRANPCCGIPAMEDARATCATIGVP
                     FYAINMEIEFGEAVIDTFVNEYAAGRTPNPCLECNRHVKFRHLVQRAKQLGADALATG
                     HYARVVHGGPERGRPHRLYRAIDGRKDQSYVLYTMTQEQLAYIQFPLGGLTKPEVRQL
                     ARAYGLPVADKEESQEICFVGNSSYAEFVSQHRPDVARPGEIVTAKGEILGHHKGLVN
                     HTVGQRRGIGVAGPKPYFVLQLKPQTNQLVVGSREQAEAQSVHVHTVSMTSGTWPEAP
                     FEVNAVVRYRGVPHAATIMPGPSGSPEMSATFHGEHGPVASPGQAIVFYRGDEVLGGG
                     TISEVARRESVSGDRPADATSVAASDRLVAQNVRER"
     gene            6812..7258
                     /locus_tag="VGR22_02710"
     CDS             6812..7258
                     /locus_tag="VGR22_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127511.1"
                     /translation="MEGAKSKGIMAGVGALLLFVLIGVGLYLLGGEEQSPLERLRDIA
                     VIFIVLASVLVVILLAGITAALAYLVFQIKDRVIPLLEQARGGMTEVTGTAKRVRNTT
                     EFVTEEAVRPLMNLTGFVSRMRTERDTVLGKNRSARKLAKKEHVGD"
     gene            7302..7880
                     /locus_tag="VGR22_02715"
     CDS             7302..7880
                     /locus_tag="VGR22_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127512.1"
                     /translation="MLGRSKKTDARARLRELEKRLDLKGLEKRLEQFEGKDLDKVLER
                     LDWKDLEKKLEKDVARRYRDLRRLGYKEEPKASSAGFFAGLVIGAVVGAVLAILFSKQ
                     NEQSPMDRFIMGPGSEQSPHSSGSETTGGGIGVATSMSGAFGDDVAIEREINGEGESV
                     TAATDAVDSASEDVRSATERVQDDPNRPAGNL"
     gene            8072..8530
                     /locus_tag="VGR22_02720"
     CDS             8072..8530
                     /locus_tag="VGR22_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEV2127513.1"
                     /translation="MDQGRYRRRATVLGVMAIVLLIAACGRATQEEIDQALGITPTPT
                     EDPAELALATEEAAAAGSNASDDPNVGVAALGRTKFQFNCQTCHSPTGTAPNLLETGG
                     PYAGIDYATLYPILREGEGHSPSPGPYAEFTLTDADIANIAAYIREQASP"
     gene            8634..9674
                     /locus_tag="VGR22_02725"
     CDS             8634..9674
                     /locus_tag="VGR22_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127514.1"
                     /translation="MVAFPIISAVLAALCALVIGRDAIRRPRPDKHAWVIAFVMFAIA
                     AGADAAGRELGWSEWLARLYYMTGPALVVAFLAIGELCLLFASRMPRVTPGAIILLTA
                     GWATLVLGAPIDEARLADEGWEAIDRSGALRVAAIAINSIGTLIIVGGLLWSIWRFQR
                     TGRFRNRMIGCIFIVSGTIAVALGGTLTRLGHYEYLYVAMSVGIALIFAGVLWSRRPD
                     RVGAGTTAGSNAGSAAARQGTEAVLPMSQEVAFLVEKVLPNEPEAIRGICLSWSVQAS
                     DDPVMSRRDARRAWRLRSHLPTMAQDRFDALSVSERRAVVELAAEVLQIPALTRVDDA
                     HRPTADPVWLEG"
     gene            9733..12189
                     /gene="mbhE"
                     /locus_tag="VGR22_02730"
     CDS             9733..12189
                     /gene="mbhE"
                     /locus_tag="VGR22_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen gas-evolving membrane-bound hydrogenase
                     subunit E"
                     /protein_id="HEV2127515.1"
                     /translation="MVKRFEGGLSRRGSPNDITTRVLSVVAGGFFLADNRAESEQDAI
                     MTWVNDPNPALVVLAAAMLAPVLTIIVGYWEPRRVSLMAIFATALAFGAALWGAAQAE
                     ASWSVSWIEAWNVNFAVTLDGLAAMYALLATGIGLLVVLYSAGYMPLHRAHAGQRSVD
                     DVRFYGFLLLFMASMVGLVLAQDLLLLFVFWDMTAIASYFLIGFDREDEESRESALMA
                     LLVTGISAIGVLIGAVILWHEYRTFSLPLIFEQMESTTPVLWAVGLILAGALAKSAQF
                     PLHFWLPRAMAAPTPVSSYLHSAAMVAAGVFLIGRIYPMVALDSRLQDALVVIGGASM
                     VVGGVVALTRDVFKQVLAYSTISQYGYVVVLFGLGGTYGIGGAMFYVIAHALAKSALF
                     MTAGSITEATGEKGLAGVGGLRDRMPLLAFGSGLAAMSVMALPLSIGFFKDELFFAAA
                     AEAGRPMQVFAVIGATLSFAYIGRLWAGWFLGGEKAQLKPVPRLLVWPVVALGLMAII
                     GGIVPGPFKGVAEGAASVSYTAPIELSLAYHLDTRPENVMALIVYALGVTVVASQNLW
                     RVPLRAVVATGQVIGPLRIYAAVLRGANALSDAIHRFEVRDLRSRIATILAPAGLLVA
                     MAVIVTPTEGAFVIGTVGKDDLPLVLVLSAAAVSALVVTFPRDHLRLALTLSIVGFSL
                     SVVYAFLGAPDVALVAVLIETLFAVVFFGMLVLMPRSILRYETSLRPERRRAARDSVL
                     ATIAGLMAFLVAWGTLSRASESTTVIRRQIEQTPLAHGYDIVTVILADFRGFDTMGEI
                     TVVAIAFLGIISLIRAGRLR"
     gene            12186..12680
                     /locus_tag="VGR22_02735"
     CDS             12186..12680
                     /locus_tag="VGR22_02735"
                     /inference="COORDINATES: protein motif:HMM:NF015970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HEV2127516.1"
                     /translation="MTTVMTQMVARLLFLPSLLIAVGVMIKGYVDVGDGFSAGVIASL
                     GVALQLMVFGAHELERLPMLRYVPAASFLGVFIALLTAFVPAIRGQALFTHWPPAGEE
                     VIHFGTLEIITAVAFDVGVFLVVFGFGVGVIGAVARAEARMFKAEESRESVGRIDGRN
                     GGVT"
     gene            12677..13141
                     /locus_tag="VGR22_02740"
     CDS             12677..13141
                     /locus_tag="VGR22_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEV2127517.1"
                     /translation="MILLLSLTVAVMFGAGAYLLLKYDLLRMVIGMVLISNAANLFIM
                     AAGLQRGDAPIYPLTDDTVSDPLVQAMTLTAIVISFSITALVLSMAYRVYTSHASVDI
                     REISQAEEVQAETDDQVGLPDPHALGKISREEEDVPDDQDMIVEGERREVRR"
     gene            13138..14514
                     /locus_tag="VGR22_02745"
     CDS             13138..14514
                     /locus_tag="VGR22_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEV2127518.1"
                     /translation="MIALPLGILWFGAVLLGVLDGTRRKNAMLSITILLLAFGATIAL
                     GFDIRQHGPREFVLGGWEQGVGIALRVDTLGIIFAVLSQAVLLAALIFETARGISARV
                     FPAMVLFMGVGLTGVFFTGDAFNFYVFFEIAMIAAYVLSSYGETPRQLRSAFIFTVVN
                     LLGSVLFLISIAALYHTTGRLDMVGIKAQVPLVDENPLILIATLMFVAFGVKLGLFPF
                     HFWLPAVYTGIRASVAAIFSGALTNIGTYGLLRFGGDLLPRELEQGGAVLIVLGSISI
                     VYGGVQSISRRTPNEVLAYSSIGQVGYIMIALAIGGELGITAAIIYAVINSLNKTLLF
                     LSVSMQGWLVGAAFAVGAFSVAGVPPFAGFLGKLVMFQAGMAADRPWAVILLVFLGGA
                     LSFLYMFQLYRRRFWIPEEQREASAMLPQAVVVVMGLGLLLVGVYPQPLIDLAGEASH
                     TITRQAAP"
     gene            14511..15005
                     /locus_tag="VGR22_02750"
     CDS             14511..15005
                     /locus_tag="VGR22_02750"
                     /inference="COORDINATES: protein motif:HMM:NF014012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HEV2127519.1"
                     /translation="MIQIILLLVVLTSTYALTVASFAWEDLAFGFGLSAVLLATFRRS
                     MLPERLQGPAMTIKAIVMLPVYLVRVIWSIITGTITVAMYVLGLQGLEHPGIVAVPIG
                     ERSAGGVAVTSLALTLSPGSFLVEVDWDERLIFVHVIDASDPDAVREELQTMYERYQR
                     HVVP"
     gene            15020..15286
                     /locus_tag="VGR22_02755"
     CDS             15020..15286
                     /locus_tag="VGR22_02755"
                     /inference="COORDINATES: protein motif:HMM:NF015993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HEV2127520.1"
                     /translation="MSDFIFYFGMVWMTVLLIITIVLVIRARTPMSRVLALDTLALVL
                     IALLVLYTTTTETSYYLDAAILLALLSFIATLAAARYYSEGRIF"
     gene            15286..15642
                     /gene="mnhG"
                     /locus_tag="VGR22_02760"
     CDS             15286..15642
                     /gene="mnhG"
                     /locus_tag="VGR22_02760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HEV2127521.1"
                     /translation="MNIAISDIFVIVGLVFMTLGVVGLIRMPDVYTKLHAASKAVFLG
                     IIVLGVAAALVGTAETGMRLILLSVLVLLTTPVSAHAIARAAYVHRNRMATAGALDES
                     GHHLADHDHAEPTWRI"
     gene            15673..15930
                     /locus_tag="VGR22_02765"
     CDS             15673..15930
                     /locus_tag="VGR22_02765"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEV2127522.1"
                     /translation="MSQTRPADTPGSFGVRFRWTPKLIAWLVLLLVSLILIFQNWDLV
                     DINVLFWGFTARLAWVLVTAFLAGALLGWAVPWLRRGVRRR"
     gene            complement(15927..16526)
                     /locus_tag="VGR22_02770"
     CDS             complement(15927..16526)
                     /locus_tag="VGR22_02770"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEV2127523.1"
                     /translation="MGEPPPIDQRSTRQPQEDRSMPSPFPTSHPPTSTAIPTVPWVIA
                     VVAVVGAITLAVWAASLRGDLDEAEARIAELAAERDQLREASTAQVFDLSPTFQGPGN
                     ANGTLYLTAEGSGVLSAVNLPELEEEDAYQVWFLPPDDGKPIPGGTFTVDDRGIGFML
                     VSADVGAIRGVSISVEPEGGSTEPTGAVLLNGSASGARG"
     gene            16778..17980
                     /locus_tag="VGR22_02775"
     CDS             16778..17980
                     /locus_tag="VGR22_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127524.1"
                     /translation="MLMISVLVLALPLASATGQVSGTPEATPTPVVGSASGQQPTPPQ
                     QQTQQQSEPATQQQQTSGPVEAPSESDTADPVIAQGLIYLTGDDVVWQVRDVDIAEPS
                     TVTGNARVILQRSGETVIRNDLTGKRTRLEPGEAYFASAGDPYTTFPDGNTPSEVWTF
                     EVSNSNEVGEGAFYLSPDVSGYGEAVYDYEFTRHTTTPGNPATIAAGDGPSMVMVLAG
                     QVNVTDENGTASLTTRDGLIVNGDATVESTSGEAVVVSMTIGPRVSDESSAPPAAAQT
                     PAEDVTTTDSDATGEETTQPVESAPPAEPSGESGAYLTSIRVGAAESIGVTLYADGVL
                     MFDGWLEPGEWTAFYDGSYFEVYTTSGENTMFENSCGGEPFFMGYETGDAYYQLEAGP
                     SSCAPVGG"
     gene            18159..18578
                     /gene="mscL"
                     /locus_tag="VGR22_02780"
     CDS             18159..18578
                     /gene="mscL"
                     /locus_tag="VGR22_02780"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEV2127525.1"
                     /translation="MLKEFREFIMRGNVIDLAVAVVIGAAFGAVVTALVETMITPLIA
                     AIGGEPDFSAISFTINDSEFRVGEFINALISFLVIAAVVFFFVVKPMNALMERVRRGE
                     ETPDPTTAVCNYCKTEIPLEATRCPHCTSADPLGATS"
     gene            complement(18663..18917)
                     /locus_tag="VGR22_02785"
     CDS             complement(18663..18917)
                     /locus_tag="VGR22_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127526.1"
                     /translation="MTIAPLHATVPTQPGTRYRVVDPRTPLPSTELAVERTVRDDLGM
                     LHVVLTDLGGREISLFVEQFEAAIVSGMLAPIPKTSPTFA"
     gene            19269..20171
                     /locus_tag="VGR22_02790"
     CDS             19269..20171
                     /locus_tag="VGR22_02790"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEV2127527.1"
                     /translation="MADAEARYTATQSLTQTVQDYVMLTKPGIQTLLLVAMLGAMLVA
                     STSVPSFELVLAMLIGGSLSSGGANALNCYIDRDIDAKMSRTRRRATATGRISPAAAL
                     AFGVTLSLLGVLVIGLAANWLAAGLALAGNLYYVLVYTLWLKRSTPHNIVVGGAAGAV
                     PPLVGWAAVTGTLSAPAWILFAIIFYWTPPHFWSLALLKQGEYGRVNVPMLPVVAGEE
                     TTRRQILLYTILLSAVSMLLVPFGMGLIYLVGIAALNTVFLWYAWLLLNDPSKARARQ
                     TFFYSLWYLALLYVVLVVDQLVVA"
     gene            complement(20385..21095)
                     /locus_tag="VGR22_02795"
     CDS             complement(20385..21095)
                     /locus_tag="VGR22_02795"
                     /inference="COORDINATES: protein motif:HMM:NF016382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HEV2127528.1"
                     /translation="MHIRNLHGWDLSVSAARAVQDELVGLVDPTTPLRLSRGDLVLGV
                     DNSYLQTATETTAFAAAIVMTWPDFEIVEVQRATRQVTFPYIPGLLTFRELPVLVEAL
                     RKVEHDPALIFSDGHGIAHPRGIGIAAHLGLLAATPTIGIAKKPFRNTWEPPGPESGA
                     HTPLYRQDGTHVGYAIRTSARRKHPVFASPGHLIRVEDVVEPVVIASGGAYLPKPQQA
                     AHDEVSHLRKALKEQKDT"
     gene            complement(21104..22498)
                     /locus_tag="VGR22_02800"
     CDS             complement(21104..22498)
                     /locus_tag="VGR22_02800"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEV2127529.1"
                     /translation="MNLTIFGTGYVGLVTGACFAESGNNVCCVDIDETKIEALRRGEI
                     PIYEPGLSELVIQNSRAGRLTFTTDATEGIDAGQIIFIAVGTPPQADGSTDLQYVETV
                     ARTIGQQLSHPAVIVNKSTVPVGTAHLVRSIIAEEMEKRGEQIPFDVVSNPEFLKEGA
                     AIEDFLRPDRIVIGAERPQSIEIMKQLYAPFNRNHDRIMVMDTRSAELTKYAANAMLA
                     TKISFMNEIANIAERVGANIENVRRGIGSDSRIGYNFIYAGIGYGGSCFPKDIRALEH
                     TASQFGYQAQLIGAVEHVNQRQKGLLLDKVLAHFEGDVEGKTFALWGLAFKPKTDDMR
                     EAPSRAIMEGLWQAGAKVRAFDPEAMNMARSLYGENPNLVLCDTRDDAVKGADALIIA
                     TEWRQFRSPDYELLRRELKTPVIFDGRNVYDPKRVAREGFTYYGIGLKSVPAKLSGDA
                     EEIDIEKLMSATAD"
     gene            complement(22658..23425)
                     /locus_tag="VGR22_02805"
     CDS             complement(22658..23425)
                     /locus_tag="VGR22_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEV2127530.1"
                     /translation="MKIVRYQQDGGAAYGILEDSTVYAASGDVFDGLTKGEAVGRVDE
                     VKLLSPIEPGKVVAIGLNYILHVKENDPTREVPTEPVVFMKPSSAVIGPGETIELAYP
                     ENNIDYEAELVVVMGKTAHRVKQDEALDYVLGYTAGNDVSDRMQQYAGQQWIRGKGYD
                     TYLPLGPCIETDLDPGNLKVESRLNGEVRQSDNTNGLIFSVPFLIEYVSDVMTLNPGD
                     IIMTGTPHGVGPMKAGDTIEVEVEGVGVLSNPVKNRD"
     gene            23611..>24264
                     /locus_tag="VGR22_02810"
     CDS             23611..>24264
                     /locus_tag="VGR22_02810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HEV2127531.1"
                     /translation="MPDLHLRLNDASEGDGPAPKPIDAATAIPLLERAEISGETLVPW
                     GSNYTFAVALCEPESDAVTQFGIYKPVRGERPLWDFPGNTLYRRERASWLLADLLGWQ
                     VVPPTVIREGPYGIGSLQLYVEPAGEMHEDDQLEFWRREMPEIERLVLFDILANNADR
                     KLTHCLVDTHGHLWGIDHGLTFNAVPKLRTVLWQFVGKPVSPELMADIVRVRLDERAL
                     "
BASE COUNT         4014 a         7433 c         8044 g         4773 t
ORIGIN      
        1 ctgcgaaccg acggcccgag cgtgccgtgg acgcctctgg agatccgtgc ggagcggcga
       61 tgcaccagcg cagcgaccgc cgtgccattc gtggtgcagg cgggaccgag cgtgctcatg
      121 attgagcagt cgcacgatgg ctgggtgctg gccgagcttc gcttcgatgg cgaggagtgt
      181 ctctttcgag atatccgggt ctcgagctac gcttggcctc gggaggcgtt cggcgtgctg
      241 ctgagccggc tggcgtcgtc cgatctcgat gcggagacgg tcgaccagtt gagcacggac
      301 ttcgccgagt gggtcgggat cggatttgca cgaattccgc agtagcccat ccattcatct
      361 gcaacacgca tgacgatccc ccggcgacac cgccgggggt ttacctgtac cgttcgattc
      421 gaacgtcgcc cgcaactcca gggctattcg tcgtcgtgag ggagaaggcc cgggtccatc
      481 tcccccgcag ggatgcggac gttcgtgatg ttctcagatg gtgggatcgg acaggcccag
      541 ccggtgttgt acgcgcagta cgggttgtac gccatgttga agtcgaccac gagccgtccg
      601 tccggtcgcg ccttcgggtc gatgtaccgc ccgaccgagt agctctcgtt gccagcggta
      661 gcgtcgcgga agggcatgaa gtagcggccg cgctcggtgt ccttgaagac cgacagcgtc
      721 gccggttgac cttcggactg gaagtgaatg cgtccgacac ggatgtagtc cttgacctcg
      781 ccgctcaacg tgccgacctg aatcgtctcc ccgattccct cgccgctctc gtcgatctcc
      841 aacgtcagat tcaagtccgg gttctcggga aaatacctga gaccctcgaa ctcggccttc
      901 tgctcctccg tgagtggcga gttggggtgc tcacggaaga aggcgttccg ccgctcgcgg
      961 taaccggcca atcgcgtgtt cgtgctcatg gttcgtcttt gcctctccag cggaatcaat
     1021 gcgacagccc aatactttat tttagatagt atagcgctct cgtcaactgg ccggatggtg
     1081 cgagggcgtt cggggtcggc agcggtgcag gtcaagcctt ggcgtgggtg tagcgatcgc
     1141 ggcactcacg gaggaccttg agggcgtgct cccgcccgtg ccacccaagg acctcggttt
     1201 ccttgtcctc gagcagcttg taggtctgga agaacgtttc gatctcgcgt agccagtgcg
     1261 gcccgacatc ctccagagcc tgatagtggg agaatcgcgg gtcgccctcg ggaacggcaa
     1321 gaatcttgaa gtcacgccct gcctcgtcgg acatgtcgag gccgccgagc ggccgagcct
     1381 cgatcatgca gccggggaag gtcggttcct gcacgagcac caggatgtcg agatggtcgc
     1441 cgtcctcggc aagggtgtcc gggacccagc cgtaatcggt ggggtagtgc accgaagagt
     1501 agagcacccg gtccagatgc cagacaccgg attcctcatc gtactcgtac ttgttgcgac
     1561 tgccccgagg aatctcgatg aaggcggaga cgagttcgcc ctggaactcc tcgaacaccg
     1621 cttcagcggt ttcgtgtatc gcttcagtat cctctgccac gtggtctgat gacgcgtcac
     1681 cctcgatctg cttcgggtct acatggctca tgcctggttg cgctccgtct tttcggcccg
     1741 gttgccctgc gaccgctgca ggaggccgac gctgtagaac tcgtacgatg cggcctggtc
     1801 cggcacatcg actagatgta ccgtgaaccg gcagggaaca ccgttgtccg gcaggtgctc
     1861 ggcaaagtat ccggcatacg cctcggcgta ggcggtccgc agccgctgct gctccgccgc
     1921 gtagccgccc gggtccacca acgggttggg cgacttcggc tggcagccga acgcgtgcat
     1981 ctcgacgaag tcgacctccc gcagtgaatc ggcgaggccg gcatcgcgca gccacttctc
     2041 ccagctgcgg aagacgagcg ggatctcacg gtggggatcc atggtcacca gctcctcgag
     2101 cccgagcacg gtcttggcgg aatacccgcc ggtcaaacct gcaaagtgga tacggagatc
     2161 accgtcgggt tgctcctccg ttaccagagc ggacagcacc ggtcgatcac cctcgtaata
     2221 gtatgtcagt ttcccacgct tcttgacacg aaaggccatg gtaccagtcg ttctccctcc
     2281 gttcggcacc cgcaagagac tcccgagcgc tcatgcaagt cgcaggccgg cttgttccgc
     2341 aagggcccgg attccggcga tcgacgctcg gctcgtcgcc tccttctcct caggtgcgga
     2401 cgcgacatgg gtttccagcg acaatgcgcc atcgtatccg gcatcggcga gcgcgcggaa
     2461 ctgaccgata tagtcgatcg cgccctcgcc catcacgacg aacgtcgggt cgggcgctat
     2521 cgccctcgcg tccttcagat gcacgtgatg aatacgcgag cggacgcgtt cgtagccctc
     2581 aggaaagggg acggagccga caaccgcctc gttgccgggg tcccaaatca tgccgatggt
     2641 gtcgtccgcg atgcggtcga gaaaccacgc agcttcgtcg cctgtggcga ccgtgcaggc
     2701 atgttcgttt tcgaagccga gcagcacccc ggagtcgcga cagatctccg ttgcctcgtc
     2761 cagggcggcg agggcaggtt ggtgaagcgt gctcgggtcg ggcgctcgcc aaaagctgaa
     2821 ggcgcgcacg atcggcgcgc caagggcatg cgcgatggtg atcgagcgac gcagcacacc
     2881 catctctcgc tggtgcgcca tctcgtcctc accccagtaa tcacatttga ggaatggaga
     2941 ggcgatggcc accacggagc agccgtgccg ctcgagggtg cgtttgacgc cagcgacggc
     3001 ggcgccatcc agctcaacga cgttttggtc agcgatcatc cggagctcga cggcgctgat
     3061 ggccaactcg ttgcagacgg taagcgcgcg gtcgaggtcg gcgctgatct catcggtgat
     3121 cactgacagg gtcggcatgc ccgttcccct tggtggagac gcgactcgag cggattgctg
     3181 gcgtgcatgc taacacccgg tgagattcgg tcaaccatct ggggcccgtc ttgccgctat
     3241 tccatgaaaa agaccggctc cagcattcgg atcgactccg cattgtcggg gtcgaccggc
     3301 gtggcgaggt agtcggtcat gaaggcttgc cagcggcggt tggcgtcgct cttagcgagg
     3361 gcccgctggg ccgcatccca gtcgtcgcat tcgagatagc cgaagagcat gggaccgtcg
     3421 gcgaagatgg agtagttgtg gtagccgggt gctcgcatgt cggcgagtag ctcaggctag
     3481 acctcgcggt ggtaggcctt gtactcgtcg agccggtcct cgcgggcgcg gaggcgaaag
     3541 gcgacgcgtt gcatgaggcg gttcctttcg atagcaacaa caggcagggg cggattccat
     3601 caggatacct gtgttgtgct tggcaaagga tcgtatgccg gatgcgaagc gcatgtgtcg
     3661 cttgcgtttg gctccgccgc accctgccgc tctgcggacg gcagggttgc tactcattcg
     3721 ccaccttgag cgggcgactt ttcggactcg acccctgcgt gaccacgatc aggacttgcc
     3781 ggtgaggagg gcctcctgga tggcgatgat gcgtgcgcgc aactgcggat cgctctccag
     3841 atccttctcg atcttctcga caccgtggag caccgttgta tggtcgcggc ccccgagcgt
     3901 gcgcccgatc tcgaccagcg agctctccgt ctcctcacgc agcagataca tggcgacgtg
     3961 gcgcggccgg acgatctcgc gcttgcgggc ccggcccagc agatccttct cctccatgcc
     4021 gaactcggca cagacggcgc ggaggacgtc ggctgcggtc gtggtggtgc ggcgctgggc
     4081 gatggaggcg tccgtgagtg cttccatggc aagctggatg tccagcttgc ggttgtagag
     4141 ttgcgccatc ataaggatct tgttgagtgc gccctccagt tcgcggatat tggtctggtc
     4201 gcgatgggcc acgtattcga tgaggtcgtc cggcagcgac acacccagct catcagcctt
     4261 ggtgcggagg atggcggtgc gcatctcgta gtccggcagc tgcacgtcgg cgatcaggcc
     4321 gccctcgaag cgtgatcgaa ggcggtcctc cagagcagag atcgacttcg gtggtcggtc
     4381 gctgctgatc acaacctgtt tgccgttctg gtagagtgcg ttgaaggtgt ggaagaactc
     4441 ctcctgcgtg ctctccttgc cggcgatgaa ctggatatcg tcgatcatca ggatatcgat
     4501 tgtcctgtac cgcgaccgga agtcctccat cttctgggcc cgaattccat tgatgagatc
     4561 gttggtgaag gtctcggagg agacgtagac gatgttgaga tccgggttgt tctcggccgc
     4621 gcggtgaccg atcgcgtgga gcaggtgggt cttgccgagc ccgacgcccc cgtagacgaa
     4681 gaaggggttg aacttgccgc cgggttgatc ggccacggcc atcgaggcag cgtgggcgaa
     4741 ccggttggac gagccgacca cgtaggtgtc ataggtcagg cgcgggttga ggccgtgcag
     4801 gccggggcgc gcgagctcca gctgcttcgt gccgagtccc ggtgattcct gcggctgctc
     4861 cttctggcgc tcgccgttcc tgcgacgctg cccggcgcgc ggaggttgcg ggcgcgggcg
     4921 ctctgagccc gccgtgggcc gaacgatgaa ctcgacgcgc agtcgctgac cggtgaggcc
     4981 gtggatcgcg cgctcgaccg ggctggcgta gcgggtctgc aacgtgttgg cggtgaaggc
     5041 gttcggcgcg ctcacgatca cctgatcgtc gtccaccgac tgcagggctg tatgtcgaaa
     5101 gaagttgtcg taggcgttgc ggttgagctc gtggcgcagt tcggtgagaa ctgtctgcca
     5161 gagctgggcg gccgcaatgc tgtcgtcggg tggactcggc ccgccggaga ccggtccgcg
     5221 ctgatcctcc actgccaatc cttcgtcgcc tgccgcagcg cgtgtcgcga gctcgatcat
     5281 gatgcgtaca tcccagtata tctgtacgta cactcttctc accatcgtac cacggcgatc
     5341 ggcaaaacca taaggggtcg gcctgcatgc tacactcgaa aggatgaaaa gacccgtgtt
     5401 cccgggcgaa tcggctccgt tcctcaggtc cgattgtggt cgggaatgca agagatttgc
     5461 gaatggtaac gtgttccctc aatcagccgg tggctctcgc accggacacc agcatcgata
     5521 ccgagccact gctctgtgaa gagcgcatcg ggccctacga cggtgccggc aagaccgcgg
     5581 tggtcgcgat gagcggcggc gtggacagtg ccgtcaccgc ccttgtcatg cgcgagcgcg
     5641 ggtaccgcgt ggtgggcatg aacctgcggc tcttctctcc cgacgatgag gaccaccgcg
     5701 ccaatccctg ctgtggcatc ccggcgatgg aggatgcccg cgccacctgc gcgacgatcg
     5761 gcgtgccctt ctacgccatc aatatggaga tcgagttcgg cgaggcggtg atcgatacct
     5821 tcgtcaacga atatgccgcg ggtcgtacgc cgaacccctg tctcgagtgc aatcggcacg
     5881 tgaagtttcg ccacctcgtc cagcgagcaa agcagctcgg cgccgatgcg cttgcgaccg
     5941 gccattacgc gcgggtcgtt catggcggtc ccgagcgtgg gcggccgcac cggctctacc
     6001 gagctatcga cgggcgcaag gaccagagct acgtgctcta cacgatgacg caggagcagc
     6061 ttgcctacat ccagttcccg ctcggtgggc tgaccaagcc ggaggtccgg cagctggcgc
     6121 gcgcctatgg cctgcccgtc gcggacaagg aggagagtca ggagatttgc ttcgtcggca
     6181 acagctccta tgccgagttc gtgagccagc atcgcccgga cgttgcgcgg ccgggggaga
     6241 tcgtgaccgc caagggcgag atactcggtc accacaaggg gctggtcaac cacacggtcg
     6301 gccagcggcg cgggatcggt gttgctgggc cgaagcctta tttcgtgctg cagctcaagc
     6361 cgcagacgaa ccagctggtc gtcggcagcc gtgagcaagc cgaggcgcag tcggtgcatg
     6421 tgcataccgt ctcgatgacg tccggcactt ggccggaggc gccgttcgag gtcaatgcgg
     6481 tggtgcgcta tcgcggtgtg ccgcatgctg cgactatcat gcccgggcca tccggctcac
     6541 ccgagatgag tgcgacgttc catggcgagc acgggccggt tgcttcgcct gggcaggcaa
     6601 tcgttttcta ccgaggagat gaagtacttg gcggtggtac gatctccgaa gttgcgcgcc
     6661 gggagagtgt ttcgggtgat cggccggccg atgcaacctc agtcgccgcg agcgaccgcc
     6721 ttgtggcgca gaacgtgcga gaacggtagc gggcaacagc gatggttggg agtgacgcct
     6781 tcgtggcgtg acaaggggag tgcgagggca tatggaaggc gcgaagagca aaggcatcat
     6841 ggccggtgtg ggagctctgc tgctgttcgt tctcatcggt gtggggctct acctgctcgg
     6901 cggtgaggaa cagtcgccgc tcgaacgact ccgggacatc gcagtgatct tcatcgtgtt
     6961 ggcctcggtc ctcgtggtga tcctgctggc cgggattacg gccgcactcg catacctggt
     7021 cttccagatc aaggatcgcg tgattccact gctcgagcag gccaggggcg ggatgaccga
     7081 ggtgaccggc accgccaaac gcgtccggaa cacgaccgag ttcgtgaccg aggaggccgt
     7141 gcgaccactt atgaacctga ccgggttcgt gtcgcgcatg cgtacggagc gggacacggt
     7201 gctgggcaag aaccgttcgg cgaggaagct cgcgaagaag gaacacgttg gcgattagtg
     7261 gttgccgctg atcgcccgct cgacagaaag tgaggatgtc gatgctgggc aggagcaaga
     7321 aaacggacgc gcgcgctcga cttcgggagc tggagaagcg cttggatctc aaggggcttg
     7381 agaagcgcct ggagcagttc gagggcaagg acctcgacaa ggtgctggag cgactcgact
     7441 ggaaagacct cgaaaagaag ctcgagaagg atgtcgcacg gcgctaccgg gatctccgga
     7501 ggcttggcta caaggaggag cccaaggctt cgtcagcggg gttcttcgcc ggactggtca
     7561 tcggcgcggt cgtcggggcc gtgctcgcga ttctgttttc caagcagaac gagcaaagcc
     7621 cgatggaccg cttcatcatg ggtcccggca gcgagcagtc gccgcacagc agcggatcgg
     7681 aaaccaccgg tggaggcatc ggtgtggcga cgtcgatgtc cggtgccttc ggcgacgatg
     7741 tcgcaatcga gcgcgagatc aacggcgaag gtgagtcggt gacggcggcg accgatgcag
     7801 tcgactcggc atccgaggac gtccgtagtg ccaccgagcg cgtgcaggac gatcccaacc
     7861 gtccggccgg aaatctgtag cgaccgttcc ggtctgatca gataacaatg gacgaccccc
     7921 gtgcggtatt ccgcgcgggg gtttggcatt gccgcccacc aaacattgac ggtcttcaca
     7981 ttcttggcta cgctagcagc gccgtacgaa cgtaaggaga caggcgacgc attgcgcgct
     8041 ggccggcaat gcgggttgca aggagttgcg cgtggatcaa ggacggtatc gccggcgtgc
     8101 gacggtgctc ggagtgatgg cgattgtgct gctcatcgcc gcgtgtggcc gagccaccca
     8161 ggaggagatc gaccaggcgc tcggcatcac tcccactccc accgaggacc cggccgaact
     8221 ggcgctcgcc acggaggagg cagccgcggc ggggtcgaac gcgtcggacg acccgaacgt
     8281 gggtgtcgcg gcgctgggca ggaccaagtt ccagttcaac tgccagacct gccactcgcc
     8341 gaccggcacg gcacccaacc tgctcgagac cggcggtccc tatgctggca tcgactacgc
     8401 gacgctctac cccattctgc gggagggtga aggacactcc ccgtcgcccg gaccatatgc
     8461 ggaattcact ctgaccgatg ccgatatcgc caacattgcg gcgtacattc gcgagcaggc
     8521 ctctccatag cgacaatccg agcccgtccc ccggacaccc gggcggtaca atgccgggga
     8581 cgatgtgacg gccggcgccg cccgggtgcc gcgtggaacg gattcctccc acagtggttg
     8641 cgtttcccat tatttcagcg gtattggccg ctctctgcgc cctggtcatt ggtcgggatg
     8701 cgatccgacg cccgcggccc gacaagcacg cttgggtcat cgccttcgtg atgttcgcca
     8761 tcgcggcggg cgcagatgcc gcgggacggg agttgggctg gtccgagtgg ctcgcccgtc
     8821 tctactacat gactgggccg gccctggtgg tcgcctttct cgccatcggc gagctctgcc
     8881 tgctctttgc cagccgcatg ccgagggtca caccaggtgc gatcatcctg ctgacggcgg
     8941 ggtgggccac gctcgtgctc ggcgctccga ttgacgaggc ccgcctcgcc gacgagggat
     9001 gggaagccat cgaccgctcg ggggcgctgc gcgtggcggc gattgccatc aactccatcg
     9061 ggacgctgat catcgtcggc gggttgctat ggtcgatctg gcggttccag cgcaccggtc
     9121 gcttccgcaa tcgcatgatc ggctgcatct tcatcgtcag tggcacgatc gcggtcgcgt
     9181 tgggcggaac gctcaccagg ctcggccact acgagtacct ctacgtcgcg atgtcggttg
     9241 ggatcgcgct catcttcgct ggcgtgcttt ggagccggcg accggatcgt gttggcgcgg
     9301 ggacgacggc aggttccaat gccgggtcgg ccgcggccag acaggggacg gaggcggtgc
     9361 tgccgatgtc ccaggaagtc gcgttcctcg tggagaaggt gttgcccaac gagccggagg
     9421 cgatccgtgg catctgcctg tcctggagcg tgcaggcaag cgacgacccg gtgatgagtc
     9481 ggcgcgatgc tcgccgcgcg tggcggttgc ggtcccatct ccccactatg gcgcaggatc
     9541 gcttcgacgc tctatcggtg tccgagcggc gcgccgtggt ggagctggct gccgaggtgc
     9601 tgcaaatccc ggcactgacc cgcgtggacg acgcgcaccg ccccaccgct gatcccgttt
     9661 ggctggaggg ataaggcggt cgagcgcggt gccatgcggc accggcgctg ctcattaccc
     9721 cgaccacccc ctatggtaaa aagattcgag ggcgggctat cacggcgtgg ctcgccgaac
     9781 gacatcacga cgcgcgtgct gtcagttgtt gctggcggct tttttctcgc tgacaatcgg
     9841 gcggaatccg aacaggacgc catcatgacg tgggtaaatg atcccaaccc agcgcttgtc
     9901 gtgctcgccg cggcaatgct ggcgccagtg ctcacgatca tcgtgggcta ctgggagccg
     9961 cgtcgtgtct cgctgatggc gatcttcgcg actgcgctcg cgttcggcgc ggccttgtgg
    10021 ggtgccgccc aggccgaggc ctcgtggtcg gtgagctgga ttgaggcctg gaacgtcaac
    10081 ttcgccgtga cgctggatgg cctcgccgcg atgtacgcac tgctggcgac cggtatcggc
    10141 ttgctggtgg tgctctattc cgccggatac atgccgctcc accgcgcaca tgccggtcag
    10201 cggtcggtcg acgatgttcg cttctacggc ttcctgctgc tcttcatggc ctcgatggtc
    10261 ggactggtgc tcgcccagga cctgctcctg ctcttcgtgt tctgggacat gacggccatc
    10321 gcctcgtact ttctgatcgg cttcgaccgc gaggacgaag agtcacgcga gtcggcgctc
    10381 atggcgctgc tcgtgacggg catcagcgcc atcggcgtgc tgatcggtgc agtgattctc
    10441 tggcacgagt acagaacgtt ttcgctgccg ctgatcttcg agcagatgga gtcgacgacg
    10501 cccgttctgt gggccgtcgg gctgatcctc gccggcgcac tggccaagag tgcgcagttc
    10561 ccgctgcatt tctggctgcc gcgggcgatg gcggcaccca ctccggtttc gtcctacctg
    10621 cactcggctg cgatggtggc ggcaggcgtg tttctcatcg gacgcatcta cccgatggtc
    10681 gcgctcgata gccgccttca ggatgccttg gtcgtcatcg gtggggcctc gatggtggtg
    10741 ggcggcgtcg tggcgctcac ccgggatgtc ttcaagcagg tactggccta ctcgacaatc
    10801 tcccagtacg gctatgtggt cgtgctcttt gggctcggtg gcacgtatgg catcggtggc
    10861 gcgatgttct acgtgatcgc gcatgcgctc gcgaagagtg cgctcttcat gaccgctggt
    10921 tcgatcaccg aggcgaccgg tgagaaggga ctcgctggcg tcggtgggct ccgtgaccgg
    10981 atgccgctgc tcgcattcgg cagcggcctc gcagcgatgt cggtgatggc gctgccgctg
    11041 tcaatcggct tcttcaagga cgagctcttc ttcgccgctg cggccgaagc cggtcgcccg
    11101 atgcaggtct tcgccgtcat cggcgcgacc ctcagcttcg cctacatcgg ccgtctctgg
    11161 gccggctggt ttctaggcgg tgagaaggcc cagctcaaac cagtcccgcg gctgctggtc
    11221 tggccggtcg tggcgctggg tttgatggca atcatcggcg gcatcgtgcc ggggccgttc
    11281 aagggtgttg ccgagggagc ggcttctgtc agctacaccg ctccaataga gttgagcctg
    11341 gcctatcacc tcgatacacg gcccgaaaac gtgatggccc tgatcgtcta tgcgcttggc
    11401 gtcactgtgg tggcgtccca gaacctgtgg cgtgtgccgc tgcgagcggt cgtggcgacc
    11461 ggccaggtga tcggtccgct tcggatttac gccgcggtcc tgcgcggcgc aaatgcgctt
    11521 tcagatgcga tccaccgatt cgaagtgcgt gacctgcgga gccgcattgc cacgatcctc
    11581 gcgccggcgg ggttgctcgt cgcgatggcc gtgatagtga cgccaaccga aggggcgttc
    11641 gttatcggga ctgttgggaa ggatgacctg ccgctggtgc tggtgctctc cgcggcggcc
    11701 gtgagcgccc tggtagtgac attcccgcgc gatcaccttc gcctcgcgct gacgctctcg
    11761 atcgtcggat tcagcctgtc ggtggtctat gccttcctcg gcgctccgga tgtggcgctg
    11821 gtcgccgtgc tgatcgagac gcttttcgca gtggtctttt tcggcatgct cgtgctgatg
    11881 ccgcgctcca tcctgcgata cgaaacgagc ctgcggccgg agcgacgccg ggcggcacgc
    11941 gacagcgtgc tcgcgacgat cgccgggctg atggcgtttc ttgtcgcatg ggggacgctc
    12001 tcccgcgcgt ccgaatccac cacggtgatc agacggcaga tcgagcagac gccgctggcg
    12061 cacggatatg acatcgtgac cgtcatcctc gcggactttc gtggcttcga taccatgggc
    12121 gagatcaccg tggtcgcgat tgccttcctt gggattatca gtctcattcg ggcggggcgg
    12181 ctcagatgac cacagtcatg acgcaaatgg ttgcccgact gctgtttctg ccgagtctgc
    12241 ttatcgccgt tggcgtaatg atcaagggct acgtcgatgt gggcgacggg ttctccgccg
    12301 gggtgatcgc atcgcttggt gtcgcgttgc aactcatggt gttcggagcg cacgagttgg
    12361 agcggctgcc gatgttgcga tacgttccgg cagcaagctt tctgggagtc ttcatcgcgt
    12421 tgctgacggc gttcgtgccg gccatccgcg gacaggcact cttcacacac tggccaccag
    12481 caggtgagga ggtgattcac ttcggcaccc tggagatcat caccgcagtc gcgttcgatg
    12541 tcggtgtgtt cctggtcgtc ttcggctttg gtgttggggt catcggtgcc gtcgctcgcg
    12601 ccgaggcacg gatgttcaag gcggaggaga gccgggagtc agtgggccgt atcgacggcc
    12661 ggaacggagg tgtgacgtga tcctcctcct ctccctgacg gtagcggtca tgtttggcgc
    12721 cggtgcctat ctgctgctca agtacgacct gctgcggatg gtgatcggca tggtgcttat
    12781 cagcaacgcc gcaaacctct tcatcatggc tgccggactt cagaggggtg acgcacccat
    12841 ctacccgctc accgacgaca ccgtcagcga tccgctcgtg caggcaatga cgcttactgc
    12901 gatcgtgatc agcttcagta tcaccgcgct tgtcctgagc atggcctacc gcgtgtacac
    12961 ctcacatgca tcggtggata tccgggagat ctcgcaggcc gaggaggtcc aggccgagac
    13021 ggatgatcag gttggcctgc ccgatcccca tgcgctcggc aagatctcgc gagaagaaga
    13081 ggacgtgccg gacgaccagg atatgatcgt cgagggtgaa cgtcgggagg tgaggcgatg
    13141 atcgcgctcc cgctcggcat cctctggttc ggggccgtgt tgctgggggt gttggatggc
    13201 acccgccgca agaacgccat gctgtcgatc acgatcctgc tgctggcgtt cggtgcaacg
    13261 atcgcgcttg gattcgacat ccggcagcac ggtcctcggg agttcgtgct cggtggctgg
    13321 gagcaagggg tcggtatcgc cctccgcgtg gataccctcg gcatcatctt cgcggtgctc
    13381 tcgcaggcgg tgctgctcgc tgccctcatc ttcgaaacgg cgcgtggcat cagtgcgcgc
    13441 gtgttcccgg ccatggtgct cttcatggga gtcgggctga ccggagtctt cttcaccggt
    13501 gatgccttca acttctacgt tttcttcgag atcgcaatga tcgcggcgta cgtgctctcg
    13561 agctacggcg agacaccgcg ccagctgcga tcggcgttca tcttcaccgt ggtgaacctg
    13621 ctcggttcgg tgctcttcct gatctccatc gctgctctct accacaccac cgggcgcttg
    13681 gatatggtcg ggatcaaggc gcaagtgcca ctggtcgatg agaacccact gatcctgatc
    13741 gccacgctca tgttcgtcgc attcggggtc aagctgggac tctttccgtt ccacttctgg
    13801 ctgccggcgg tctacaccgg catacgggcg tccgtcgcgg cgatattcag cggcgcgctt
    13861 accaacatcg gcacgtatgg gctccttcgg ttcggtggcg acctgctgcc gcgcgagctg
    13921 gagcagggag gggcggtgct catcgtgctt ggcagcatca gcatcgtgta cggcggggtg
    13981 cagtcgatct cgcggcggac tcccaacgag gtgttggcct actcgtccat tggacaggtg
    14041 ggctacatca tgatcgcact cgccatcggt ggggagttgg gtatcactgc cgcaatcatc
    14101 tacgcagtga tcaacagcct gaacaagaca ctcctctttc tctcggtgag catgcagggc
    14161 tggctggtgg gggcggcgtt cgccgtcggc gccttcagcg tggccggcgt accgccgttc
    14221 gccggcttcc tcggcaagct ggtgatgttt caggccggca tggcagccga tcggccctgg
    14281 gcggtcattc tgctcgtttt cctcggcggt gcgctctcgt ttctctacat gttccagctc
    14341 taccggcgcc gcttctggat cccggaagaa cagcgtgaag cgtccgcaat gctgccgcag
    14401 gcggtcgtgg tggtgatggg gctcgggttg ctgctggttg gggtgtatcc gcagccgctg
    14461 atcgatctgg cgggagaggc gagtcacacg atcacgaggc aggcggcgcc atgattcaga
    14521 tcattctcct cctcgtggtc ctgaccagca cctatgcctt gaccgttgca agcttcgctt
    14581 gggaggacct agcttttggg tttggcctct ccgccgtgct gctggccacc ttccgccgct
    14641 cgatgctgcc agagcgactg cagggcccgg cgatgacgat caaggcgatc gtgatgctgc
    14701 cggtctactt agttcgcgtg atctggagca ttatcaccgg gaccattacg gtggccatgt
    14761 acgtgctcgg cttacagggg ctcgagcatc ccggcatcgt cgccgtgccg attggggagc
    14821 gctcggcggg gggtgttgca gtgacttccc tggccctgac cctctcaccg ggttcgttcc
    14881 tcgtggaagt ggactgggac gagcggctga tattcgtgca cgtgatcgac gccagtgatc
    14941 cggatgcggt tcgcgaggag ctgcagacga tgtacgagcg atatcagcgg cacgtggtgc
    15001 cgtagcgaag gcggagagca tgagcgactt catcttctat ttcggcatgg tgtggatgac
    15061 agtactcctg atcatcacga tcgtgctcgt catccgtgcg aggacaccga tgagccgcgt
    15121 gctggcgctc gacacgctcg cgctggtgct gatcgcgctg ctggtcctct acaccaccac
    15181 gacggaaacg tcctactacc tcgacgcggc gatcctgctg gctctgctgt cgtttatcgc
    15241 cacgctggca gcagcccgct actacagtga ggggcgcatc ttctgatgaa catcgcgatc
    15301 tcggacattt tcgtgatcgt cggcctggtt ttcatgaccc tcggggttgt ggggttgatc
    15361 cgaatgcccg acgtgtatac caagctccac gccgccagca aggccgtctt cctcggcatc
    15421 atcgtgctgg gcgttgctgc cgccttggtt gggacggcgg agacaggaat gcgcctgatc
    15481 ctcttgagtg tgctggtgct gctgacgaca cctgtgtccg cccatgcgat cgctcgggct
    15541 gcctatgtcc accggaatcg aatggccacg gcgggcgcgc tcgacgaatc gggccaccac
    15601 ttggcggacc acgatcacgc cgaaccgact tggcgcatat agcggcttga ctggccggaa
    15661 agagaagtga acatgtccca gacgagaccc gccgacactc ccggctcttt cggtgtccgg
    15721 ttcaggtgga ccccgaagct gatcgcgtgg ctggtactgc tgctggtatc gttgatcctg
    15781 atcttccaga actgggatct tgtcgatatc aacgtgctgt tctgggggtt caccgcccgg
    15841 ctcgcatggg tccttgtcac ggcgttcctg gccggggcct tgctcggctg ggccgtgcct
    15901 tggctgcggc ggggcgtgcg acgtcgctaa ccgcgtgcac cactcgccga gccgttgagc
    15961 agcaccgcgc cggtcggctc cgtgctgccg ccttcgggct ccacggaaat cgacactccc
    16021 cggatcgcgc cgacatccgc cgagacgagc atgaagccga taccccggtc gtccacagtg
    16081 aaggtgcccc ctgggattgg cttgccgtca tcgggcggca gaaaccacac ctggtaggca
    16141 tcttcctcct ccaactcggg aagattgact gcgctcagca caccggaccc ctccgcggtc
    16201 agataaagcg tgccgttcgc gttgcccggc ccttggaatg tcggtgagag gtcgaagacc
    16261 tgcgctgtcg atgcctcccg gagctggtca cgctcggcgg cgagctcggc aatacgcgcc
    16321 tccgcctcat caaggtcgcc gcggagcgag gctgcccaca cggccaaggt aatcgcgccg
    16381 acgaccgcta ccacggcgat aacccatggg actgtcggta tggcagtgga ggttggcggg
    16441 tgggaggtcg ggaagggtga gggcatcgag cgatcctctt gtggttgacg agttgaacgc
    16501 tgatcaatag gcggtggttc gcccacacaa ctgtacccag atcgcccatg tgatgctcgt
    16561 caacgtgcgt ctggacactt ggtactatgc tgatagcact gtacgattcg ccgcactatc
    16621 cagaaggctg ccggccgcgc acgcgccgga cgcgggaaag atccgaggag gaccggcgcg
    16681 ggtggcacag tggtgagtag agactgacga catgaagcaa aagggaacgg ttgtgattac
    16741 atacggtcgc cgctggacaa gcgggagaac gctctgcgtg ctcatgatca gcgtgctggt
    16801 cttggcactg ccgctcgctt cggccacggg gcaggttagc ggaacgccgg aggcgacccc
    16861 aacgccggtg gtcggctcag cctctggtca acagcctacg cccccacagc agcagaccca
    16921 gcagcaatcc gagccggcaa cccagcagca gcagacgtcc gggccggtgg aggctccctc
    16981 ggagagtgac acggccgatc cggtgattgc gcaagggttg atctatctca ctggagatga
    17041 tgtcgtctgg caagtgcgtg acgtcgacat cgccgagccg tccaccgtta ccggcaatgc
    17101 acgcgtgatc ctgcagcggt ccggcgagac cgtgatccgc aatgacctga ccggaaagcg
    17161 gacacgactg gaaccaggtg aggcctactt cgcgtcggcg ggggatccgt acacgacgtt
    17221 cccggacggc aacacgccct ccgaggtctg gaccttcgag gtttccaaca gtaacgaggt
    17281 cggggagggc gccttctacc tcagcccgga cgtctccggc tatggcgagg ccgtttacga
    17341 ctacgagttc actcgccaca ccaccacgcc cggtaacccg gcgacgatcg ccgctggtga
    17401 cggtccgtcg atggtgatgg tgctcgccgg tcaggtcaat gttaccgacg agaacggcac
    17461 cgcgtcgctc acgacgcggg acggcctgat cgtgaacgga gacgcgacgg tcgaatcgac
    17521 cagcggcgag gccgtggtag tctcgatgac catcgggccg cgggtgtcgg acgaatcgtc
    17581 tgcgccaccc gcagcggcgc agacgccggc agaggacgtg accacgacgg acagcgatgc
    17641 aacgggcgag gagaccacgc agcctgttga gagcgctccg ccggccgagc cgagcggaga
    17701 gtccggggcg tatctcacca gcatccgtgt tggtgctgca gagagcatcg gggtgacgct
    17761 ctacgccgac ggtgtgctga tgttcgatgg ctggctggaa ccgggagagt ggaccgcctt
    17821 ttacgacggt tcatacttcg aggtctacac cacctccggt gagaacacga tgttcgagaa
    17881 ctcgtgtggt ggcgagccgt ttttcatggg gtacgagacc ggcgacgcct actaccagct
    17941 ggaagctggc ccgagttcct gtgcgccggt tggcggctga gccggcgtcg cctgggatcc
    18001 acgaagcgtg atggccggcg ccggggtctc cctggcgccg gctgatcgtc tgcaagagtt
    18061 tcgctgccga cgcgcctgaa tgaggggtgg acgatcagag agcgggcggc gcagtatagg
    18121 gtggtggtgc tgaccgacag cttggcatgg gaggggcgat gctgaaggag ttccgcgagt
    18181 tcatcatgcg cggcaacgtg atcgaccttg ccgtcgccgt ggtgatcggc gccgccttcg
    18241 gggccgtcgt gaccgccttg gtcgaaacga tgatcacgcc gctgatcgcc gcaatcggcg
    18301 gggagccgga cttcagcgca atctccttca ccatcaatga cagcgagttt cgtgtcggcg
    18361 aattcatcaa cgcgctgatc tcgtttctgg tcatcgccgc ggttgtcttc ttctttgtcg
    18421 tcaagccgat gaacgcgctc atggagcgtg tccggcgcgg cgaggagact ccggacccca
    18481 cgacggcagt ctgcaactac tgcaagacgg agatcccgct ggaagccacg cgctgcccac
    18541 actgcacctc ggcagatccg ctcggtgcga cgagctaatg tgcaggcatc gacacggcaa
    18601 cggaccggct cctcaggagc cggtccgttt actttcctgc tggccggaat agacgaaaac
    18661 cgctacgcga acgtcggaga cgtcttgggg atgggagcaa gcatgccgct gacgatcgcc
    18721 gcttcgaact gctcgacgaa caggctgatc tcccggccac cgaggtcggt cagcaccacg
    18781 tgaagcatgc cgagatcatc tcggacggta cgctccactg ccagttcggt gctcggtagc
    18841 ggcgttcggg ggtcaacgac acggtatcgg gtgccgggct gagtcggaac ggtagcatgg
    18901 aggggagcta tcgtcactag cggtctcctt cagtggatcg atgcgtcgtt gcatattacg
    18961 agcggctctg aggacgttca ggggaattcg tgaatcattt cacatactag atacaggccg
    19021 gcaagttgcc tgtcaacccg acctctgtat acatgaaccg ggccagtgat ttcgacgcgc
    19081 ttgcgctacc gttcgcaagc ggatttacgg tatggattcg gacaccggcc tgcaacgcga
    19141 tgtgcaatta cccacgacgc cgctacactg gtacgcgaca gggcgccgcc atcgtcgcgc
    19201 gcctcgaccc cgaaacagca cagttgcgcc gccatcacgg ccggcgtgcc gatggagacg
    19261 cacggaacat ggcggatgca gaggcccgat acacggcaac acagagcctg acacagaccg
    19321 tccaggacta cgtcatgctc acgaagccgg gtatccagac gctgctgctg gtggcgatgc
    19381 tcggggcgat gctggttgcc tcgaccagcg tgccatcgtt cgagctggtg ttggcgatgc
    19441 tgatcggtgg ctctctttcc tcgggcggcg cgaatgcgct caactgctac atcgaccgtg
    19501 acatcgacgc gaagatgagc cgcacgcgga ggcgggcgac ggccaccggg cgcatctcac
    19561 cggccgctgc gctggctttc ggcgtgacgc tgtcactcct cggcgtgctg gtgataggtc
    19621 ttgccgccaa ctggctcgct gccgggctcg cgcttgccgg caacctctac tacgtcctcg
    19681 tctacacact ctggctcaag cgttcgacgc cgcacaacat cgtcgtcggc ggcgcggcgg
    19741 gtgcggtgcc accgctcgtc ggctgggccg cggtgaccgg caccctctcg gcaccggcgt
    19801 ggattctctt cgcgatcatc ttctactgga ccccgccgca cttctggtca ctggcgctgc
    19861 tcaagcaggg cgagtacggt cgcgtgaacg tgccgatgct gccggtcgtt gctggagagg
    19921 aaactacccg ccggcagatc ttgctctata ccatcctgct gtcggccgtc tcgatgctgc
    19981 tggtgccatt cggcatgggg ctgatttatc tggtcggcat cgcggcgctg aacacggtgt
    20041 tcctctggta cgcatggctg ctgcttaatg atccgagcaa ggcccgcgca cgccagacgt
    20101 tcttctactc gctgtggtat ctggcgctgc tttacgtggt gctcgtggtc gaccagttgg
    20161 tggtggcgta gcgaacgtca ttggcacgtg atatcgcaat aggcgtcatt ctgagccggc
    20221 tgcggcaccg taggtgccgc agccgtgtcg caggatctct cggccctggc agcggcaagg
    20281 gcggtcttgt tcattcgcat ctgacagtac gcgagtcgtg aactgacacc acgcgaggga
    20341 tacgggccgg gcggcccttc gactccggcc cttccaccgc gtcgctatgt gtccttctgc
    20401 tccttcaagg ctttgcggag atggctgact tcatcgtgcg cagcctgctg gggtttcggc
    20461 agatacgcac cgcccgatgc gatcaccacc ggctcgacca cgtcttcaac gcgaatcagg
    20521 tggccgggcg aggcgaagac cggatgtttg cggcgcgcgc tggtgcgtat ggcgtagccg
    20581 acgtgcgtgc cgtcctgcct gtagagcggc gtgtgcgccc cggattcggg gcccggcggc
    20641 tcccacgtgt tgcggaaggg ctttttggcg atgccgatcg ttggggtagc ggcaagcaga
    20701 ccgagatggg cggcgatgcc gatgccgcgc gggtgagcga tgccatgccc gtcgctgaag
    20761 atcagggcag gatcgtgctc cacctttcga agcgcctcga ccagcaccgg cagctcccgg
    20821 aaggtgagta gacccggaat gtaggggaag gtaacctgtc gggtggccct ctggacctca
    20881 acgatctcaa agtcgggcca cgtcatgacg atcgcggccg cgaatgccgt ggtctccgtt
    20941 gcggtctgca aataggagtt gtcgacgccg agcaccaggt caccgcgaga gagacgcagc
    21001 ggtgtggttg ggtcgaccag gccgacgagc tcgtcctgca cggcgcgagc cgcggagacg
    21061 ctcagatccc agccgtgtag gttgcggata tgcatggccg aggctaatcg gcggtggccg
    21121 acatgagctt ctcgatgtcg atctcctccg cgtcccctga gagcttcgcc ggcactgact
    21181 tgaggccgat gccgtagtag gtgaaaccct cgcgggcgac ccgcttcggg tcatacacgt
    21241 tgcggccgtc gaaaatgaca ggcgtcttca gttcccggcg aagcaactcg tagtcagggc
    21301 tgcggaactg acgccactcg gtcgcgatga tcagggcgtc agcacccttc accgcgtcgt
    21361 cgcgggtgtc gcagagcacg aggttggggt tctcgccata cagcgaccgc gccatgttca
    21421 tcgcctcggg atcgaaggcg cgcaccttgg cgcccgcctg ccagagcccc tccatgatgg
    21481 cgcggctcgg cgcctctcgc atgtcgtcgg tcttcggttt gaaggcgaga ccccaaaggg
    21541 caaaggtctt gccctccacg tccccctcga agtgagccag caccttgtcg agcaacagcc
    21601 ccttctggcg ttggttgacg tgctcgacgg cgccgatcag ttgtgcctgg taaccgaatt
    21661 gggaggcagt gtgctcaagg gcccggatgt ccttcgggaa gcaggagccg ccgtagccga
    21721 taccggcgta gatgaagttg tagccgatgc gggagtcgga gccgatgccg cggcggacgt
    21781 tctcgatatt ggcgccgacc cgctcggcga tgttggcgat ctcgttcatg aaggagatct
    21841 tggtggccag catcgcgttg gcggcatatt tggtcagctc tgccgagcgg gtgtccatca
    21901 ccatgatgcg atcatggttg cggttgaacg gcgcgtagag ctgcttcatg atctcgatcg
    21961 actgtggccg ctcggcacca atgacaatgc ggtccgggcg caggaaatcc tcgatcgccg
    22021 cgccctcctt gaggaactcc gggttgctga ccacgtcgaa cgggatctgc tcgccgcgct
    22081 tctccatctc ctcggcgatg atgctgcgca ccagatgggc ggtgccgacc ggtacagtgg
    22141 atttgttgac gatgacggca gggtgggaca actgctggcc gatcgtccgg gcgactgtct
    22201 cgacgtactg gaggtcggtc gagccgtctg cctgcggcgg cgtgccgacg gcaatgaaaa
    22261 tgatctggcc tgcgtcgatt ccctcggtag cgtcggtggt gaaggtgagt cggccggcac
    22321 ggctgttctg gattaccagc tcacttagcc caggctcgta gatgggaatc tcgccgcggc
    22381 gcagcgcttc gatcttcgtc tcatcgatat cgacgcagca aacgttgttg ccgctttcgg
    22441 cgaagcaggc gccggtcacc aggccgacat agccggtgcc gaagatcgtc aggttcatgc
    22501 ggtgtctcct cgaagcaccg gagtgcgttg ttgacaggat agtgaagcaa ggcacagatc
    22561 ggctttacag acacctaagg taccaaaaag gggcagcaca acacagccgg taccagagcg
    22621 gctccgggct gtgtgcttgc ggcttgtcat tcggcggcta gtcgcggttt tttaccgggt
    22681 tgctcagcac accgacaccc tcgacctcga cctcgatcgt atcgccggcc ttcatcgggc
    22741 cgacaccgtg tggggtaccg gtcatgatga tgtccccggg attaagcgtc atcacgtcgc
    22801 tgacatactc gatcaggaat ggcacgctga agatcaggcc gttggtgttg tcagattggc
    22861 ggacctcccc gttgaggcgg gattcgacct tgaggtttcc ggggtcgagg tccgtctcga
    22921 tgcatggtcc gagcggcagg taggtgtcat agcccttgcc gcggatccac tgctggccag
    22981 cgtactgttg catgcggtcc gagacgtcgt tgccggcggt gtagcccaac acgtaatcca
    23041 gcgcctcgtc ctgcttcacg cggtgcgccg tcttgcccat caccacgacg agctcggcct
    23101 cgtagtcgat gttgttttcg gggtaggcga gctcgatcgt ctcgccggga ccgatgaccg
    23161 ccgatgaagg cttcatgaag accaccggtt cggtcggcac ctcgcgagtc ggatcgttct
    23221 ccttgacgtg gagaatgtag ttgagcccga tcgcgacgac cttgcccggc tcgatcggcg
    23281 agagcagctt cacctcgtcg acgcggccga ctgcttcgcc ctttgtcaag ccgtcgaata
    23341 cgtctccgct cgcagcgtag acggtcgaat cctcgagaat gccgtaggcg gctccgccgt
    23401 cctgctgata ccgtacgatc ttcatcgaag ctttctcctt cccccgcggg gatgctccgg
    23461 cgcattggcc ggtcaccgtg cgatttcccg tccggccgat tgtatatgca gcgtgacaat
    23521 cgcgcgatgg tacgccgtga taggcgggta cacttggagc caggaccgcg cggtcctcac
    23581 cgagccgacg agccaccccg gaacgccgcg atgcccgatc tccacctccg cctcaacgac
    23641 gccagtgagg gcgatggccc agcgccgaag ccgatcgacg cggccacggc gatcccgctg
    23701 ctggagcggg ccgagatatc aggagagacg ctggtaccat gggggtccaa ctacaccttc
    23761 gcggtcgcgc tctgcgagcc ggagtcggac gcggtgaccc agttcggcat ctacaaaccg
    23821 gtgcggggcg agcggccgct ctgggatttt cccggcaaca cactctatcg ccgcgagcgt
    23881 gcctcttggc tgctcgccga tctgctcggc tggcaggtcg tgccgccgac ggtgatccgg
    23941 gagggtccat acgggatcgg cagcctccag ctctatgtgg agccggccgg ggaaatgcat
    24001 gaggatgacc aactggagtt ttggaggcgc gagatgcccg agatcgagcg gctcgtgctc
    24061 ttcgacatcc ttgccaacaa cgccgatcgg aagctgacgc actgcctggt tgacacccat
    24121 ggccacctgt ggggcatcga ccacgggctg acgttcaacg cggtgccgaa gctccggacg
    24181 gtcctatggc agttcgtcgg caagccggtg tcgcccgagt tgatggcaga catcgtccgt
    24241 gtgcgcctcg acgagcgggc gctg
//
LOCUS       DASYWL010000075         2891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_622761, whole genome shotgun sequence.
ACCESSION   DASYWL010000075 DASYWL010000000
VERSION     DASYWL010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622761"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1677
                     /gene="ftsH"
                     /locus_tag="VGR22_02815"
     CDS             <1..1677
                     /gene="ftsH"
                     /locus_tag="VGR22_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEV2127532.1"
                     /translation="VEIEVEPASRWGAIFSGLAIFLPILFLVGIFLLIMRQSQGGSNQ
                     AMSFGKSKARLFSSNRPSVTFSDVAGVDEAKEELAEVVDFLKYPEKFASLGARIPRGV
                     LLVGPPGTGKTLLSRAVAGEAGVPFFSISGSEFVEMFVGVGASRVRDLFEQAKRSSPC
                     IVFIDEIDAVGRQRGAGLGGSHDEREQTLNQILVEMDGFDTSTNVIIIAATNRPDVLD
                     PALMRPGRFDRQVILDRPDIAGRRSILQVHTRGKPVESDVSIENLARQTSGFSGADLE
                     NLVNEAAILAARRNKKTVGRPELTEAIDRVIAGPQRKSRVISERERLMTAYHEAGHAL
                     VARMLPHADPVRKVSIVARGMAGGYTRMVPDEDRLFKTKAQFEDDIAVFMAGQIAEQL
                     VFEEISTGASNDIERATAIARRMVTEFGMSQLGPLAFGKKDEMVFLGREISEQRNYSD
                     EVAFKIDQEVRQIVDRGHARALEIIQTQFDRLEAIAQLLLREETIEDEELEALFDSPR
                     PKPDLVGPPTGRPSRIARKVADVRGRPQDDQRDQEDDDGGSFGTLTPQPI"
     gene            complement(1745..1817)
                     /locus_tag="VGR22_02820"
     tRNA            complement(1745..1817)
                     /locus_tag="VGR22_02820"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1782..1784),aa:Ala,seq:cgc)
     gene            2060..2135
                     /locus_tag="VGR22_02825"
     tRNA            2060..2135
                     /locus_tag="VGR22_02825"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2093..2095,aa:Gly,seq:ccc)
     gene            2373..>2891
                     /locus_tag="VGR22_02830"
     CDS             2373..>2891
                     /locus_tag="VGR22_02830"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEV2127533.1"
                     /translation="MGRIERFRDDRIGMFIHWGSYSTKGFEASWPLVRGEISYDEYEA
                     MADRFNPQRYGPAEWAELAKAAGMRYAILTTKHHDGFALYDTQLSDYSAPNRAAGRDL
                     IRPYVEAFRAAGIQVGFYFSLCDWHHPDYPSQLSDPRPERSRSPQALPPGVPESIAAD
                     PARWDSYIEFMHG"
BASE COUNT          549 a          886 c          936 g          520 t
ORIGIN      
        1 gtggagatcg aggtggagcc tgcgagtcgc tggggcgcta tcttcagcgg gctggcgatc
       61 ttcctgccga tcctcttcct cgtgggtatc ttcctgctga tcatgcgcca gagccagggc
      121 ggcagcaacc aggcgatgtc gttcggcaag agcaaggccc ggctcttctc cagcaaccga
      181 ccatcggtga ccttctccga tgttgctggc gtcgacgagg caaaggagga gctggccgag
      241 gtcgtcgact tcctgaagta ccccgagaag ttcgcctccc tgggcgcgcg catcccgagg
      301 ggcgtcctgc tggtcggccc gccgggcacc ggcaagaccc tgctctcccg cgcggtcgcc
      361 ggtgaggccg gcgtgccgtt tttcagcatc tcgggctccg agttcgtcga gatgttcgtt
      421 ggtgtcggcg ccagccgcgt ccgcgacctc tttgaacagg ccaagcgcag ctctccctgc
      481 attgtgttca tcgacgagat cgacgctgtc ggtcggcagc gcggagccgg cctcggcggc
      541 tcccacgacg agcgcgagca gacgctgaac cagatcctcg tcgagatgga cggcttcgac
      601 actagcacca atgtgatcat catcgcggcc accaaccggc cggacgtgct tgaccctgcg
      661 ctcatgcgac cgggtcgctt cgatcggcag gtcatcctcg accgtcctga catcgcaggg
      721 cggcgctcga ttctgcaagt gcacacgcga ggcaagccgg tggagtcaga tgtctcgatc
      781 gagaacctcg cgcgccagac atcgggcttc tccggcgccg acctcgagaa cctcgtcaat
      841 gaggcggcga ttctggcggc acggcggaac aagaagacgg tcgggcgacc cgagctgacg
      901 gaggcgatcg accgtgtgat cgccggaccg cagcgcaaga gccgagtgat ctccgagcgg
      961 gagcgtctga tgacggcgta ccacgaggct gggcacgcgc tggtggcgcg aatgctgccg
     1021 catgccgatc cggtgcggaa ggtgtcgatc gtcgcgcgcg gcatggcggg tggctacacc
     1081 cggatggtgc ccgacgagga tcggctcttc aagacgaagg cgcagttcga ggacgatatc
     1141 gccgtcttca tggcggggca gatcgccgag cagctggtgt tcgaggagat ctccaccggc
     1201 gcgtccaacg acatcgagcg cgccacggcg atcgcgcgcc ggatggtgac cgagttcggg
     1261 atgagccagt tggggccgct cgccttcggc aagaaggacg agatggtctt cctcggtcgc
     1321 gagatcagcg agcagcggaa ttacagcgac gaggtggcct tcaagatcga ccaggaggtc
     1381 cggcagatcg ttgatcgcgg acatgcccga gcactggaga tcatccagac gcagttcgac
     1441 cggctggagg cgattgccca gctcctgctt cgggaagaga cgatcgagga tgaggaactc
     1501 gaggccctct tcgactcccc gcggccgaag ccggacctcg tcggtccgcc gaccggacgg
     1561 ccgtcgcgga ttgctcggaa ggtggcggac gtgcggggcc gaccgcagga cgaccagcgc
     1621 gatcaggagg acgacgatgg cggatcgttc ggtaccctga cgccccagcc gatctgacag
     1681 cagcagaatg gcacgaagaa cggccgtggc atcatgccac ggccgtttca tttcgcgttc
     1741 ggggtggaga cggggggatt cgaacccccg acctctacag tgcgattgta gcgctctccc
     1801 agctgagcta cgtccccgaa agcgtctgga gtatagcccg tcggaccggt gtcgtcaatg
     1861 cgttcgatac atcggcacat cgccgaggtg atccgcggct cccgtgcgga gatcgtcggc
     1921 tgatgggcga acgcgcgttg cctcggatca ttacccggtc gtcgctgacc tcggaattac
     1981 ccgagcattg aattccgtat cgaatcccag gggtaccaat cggtcaaaag cagtggtata
     2041 cttgtgtccg gacaccggtg cgggtgtaac tcagttggta gagtgtctgc ttcccaagca
     2101 gaatgtcgtg ggttcgagtc ccatcacccg ctccaaacgg caaaacaggc ccagcgacgc
     2161 caaacggcgc tgggcctgtt cccgtatagg ggctgccaca acgttcaacg acatctggct
     2221 cgtggcctga cattcggggc agtgaagggg taaccgctac ctgttacgaa tcgagaaagg
     2281 agattcgtct cccacaagag taggcttgcc tgaaccgtca cgggcgcact ggcagaacgt
     2341 atacgccata ggcgagtcgg aggaaaccaa tcgtgggtag gattgaaagg ttccgggacg
     2401 atcgtatcgg tatgttcata cactggggct catacagcac caaaggcttc gaagcatcgt
     2461 ggccactggt ccgcggcgaa atctcctacg atgagtatga ggccatggca gatcgcttca
     2521 acccgcagcg ttacggtcca gcggagtggg ccgaactggc gaaagcggcg ggtatgcgct
     2581 atgccatcct cactactaag caccacgatg gtttcgcgct ctacgacact caattgagcg
     2641 actattcggc gcccaatcgg gcagctggtc gcgacctcat ccgtccctat gtcgaagcct
     2701 ttcgtgcagc tgggatccaa gttgggttct acttctcact ctgcgactgg caccacccag
     2761 actacccctc gcaactcagt gacccacggc cagaacgctc gcgctctccg caggcactcc
     2821 cacctggcgt gccggagagt attgcggctg acccggcacg atgggacagc tatattgagt
     2881 tcatgcatgg t
//
LOCUS       DASYWL010000076         2318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_397621, whole genome shotgun sequence.
ACCESSION   DASYWL010000076 DASYWL010000000
VERSION     DASYWL010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397621"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VGR22_02835"
     CDS             complement(<1..393)
                     /locus_tag="VGR22_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127534.1"
                     /translation="MPSATSRASASPIRGITKTHAVLLALVVLAFNLRAPMLAVPPVL
                     ETIQRDLGLSGIAAGMITTLPVLCFGLVSPLAPVLARKLGLDTTLMVAMTAIAAGVIV
                     RMGQPALALYLGTLGLGAGIALLNVLIPA"
     gene            complement(498..821)
                     /locus_tag="VGR22_02840"
     CDS             complement(498..821)
                     /locus_tag="VGR22_02840"
                     /inference="COORDINATES: protein motif:HMM:NF025742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEV2127535.1"
                     /translation="MAKREQIVSYTAEEIDEMIARGEDQTDWERVRTMTDEEAEAAID
                     HEEEGEFDWNAAYVGSAPAKQQITVRFDTDIIEWFKENGPGYQTRMNQVLRAYIEAQR
                     NKKAS"
     gene            complement(939..1226)
                     /locus_tag="VGR22_02845"
     CDS             complement(939..1226)
                     /locus_tag="VGR22_02845"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEV2127536.1"
                     /translation="MGEARAEFEWDEDKRRSNIAKHGIDFVRARAVFDGRPLAHIPSP
                     VPHEARTMTIAILDDVHVAAVWTERDGKRRFIAVRRASRSERTLYQALTQP"
     gene            complement(1448..2023)
                     /locus_tag="VGR22_02850"
     CDS             complement(1448..2023)
                     /locus_tag="VGR22_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127537.1"
                     /translation="MSTDQKHKVIADWIGDVVALESHVEEALDHQLKLKSDSTALTSA
                     IQRFHDTVRDSKRRAVSYEETYGTQASKGIIERGSELLGKAAGIIDRLRHDSVSKALR
                     DDFTAFNHTAMAYQMLHATAMALGDSATEAFAGEGLRTYATLVQDVNQVISEAVILDL
                     QGSPDYPDVDTTAIDHCRNFINQTWKATSNT"
BASE COUNT          341 a          770 c          758 g          449 t
ORIGIN      
        1 agcggggatc agcacgttga gcagtgcgat gccggcgccc agcccgagcg tgccgaggta
       61 gagcgccagc gccggctgtc ccatgcggac gatgaccccc gccgcgatcg cggtcatcgc
      121 gaccatcagg gtggtgtcga ggccgagctt tcgcgccagc accggcgcca gcggcgagac
      181 cagcccgaag cagagcaccg gcagggtcgt gatcatcccc gccgcgatgc ccgagaggcc
      241 gaggtcgcgc tggatcgtct cgagcacggg cggcacggcc agcatcgggg cgcgcaggtt
      301 gaaggcgagc acgacgagcg cgagcagcac cgcgtgggtc ttggtaatgc cgcggatggg
      361 cgaggcgctc gcccggctgg tggcggaggg catggacggc gggttccggt gagtgatcga
      421 cagacatgac gaccacccgc cgcgcggacg ggtggtcgct tcactgtacc gcagtgatcg
      481 gggtatgggc tgtggggtca ggacgccttc ttgttgcgct gcgcctcgat gtaggcgcgc
      541 aggacctggt tcatgcgggt ctggtagccc ggcccgttct ccttgaacca ttcgatgata
      601 tcggtgtcga accggacggt gatctgctgc ttcgccgggg ccgagccgac gtaggcggcg
      661 ttccagtcga actcgccttc ttcttcgtgg tcgatcgccg cctccgcttc ttcgtcggtc
      721 atggtcctga ctcgttccca atcagtttgg tcctcgcccc gcgcgatcat ctcgtcgatc
      781 tcctcggccg tgtagctgac gatctgttca cgctttgcca tggcaatgct ccgccgttcg
      841 tgaaccgggc acatggtgta gttacattta taactacacc gcacagccat tgtatcgaat
      901 cggtcgatcg atcgttggat gcctgagagc ccccggaatc atggctgtgt caatgcctga
      961 tacagggtgc gctcgcttct gctggcgcgt ctgacggcga tgaagcgcct tttgccatct
     1021 cgctccgtcc acactgccgc tacatggaca tcgtccagga ttgcgatggt cattgttcgc
     1081 gcctcgtggg gaaccgggct cggtatatgg gcgagcggcc gtccatcgaa gaccgccctc
     1141 gctcgaacga agtcgatgcc gtgcttggcg atgttgcttc gccgcttgtc ttcatcccac
     1201 tcgaactccg cccgtgcctc tcccacgcac ttggcaccgc cgacctggcc gatgtgctca
     1261 ggttcgcatg agattgctgg aacaggtgcc ttggcgcccc gagaatcgac tgtctgctgg
     1321 cacaacgatc ccgcgcaggg agagggagcc tgcacggttg ccatcagctc cttgggcatg
     1381 acaagcccgg cgggcggggc gtgccccacc ggccgggctg cttgtactct ctgctgtcgg
     1441 gcagggccta cgtgttgctg gtggccttcc aggtctggtt gatgaagttc cgacagtggt
     1501 cgatcgccgt cgtgtcgacg tcggggtagt ccgggctgcc ctgcaggtcg aggatcaccg
     1561 cctcggagat cacctggttg acgtcctgca ccagcgtggc gtaggtccgc agcccttcgc
     1621 cggcgaacgc ctcggtcgcg ctgtcgccca gcgccatcgc cgtcgcgtgc agcatctggt
     1681 acgccatcgc ggtgtggttg aacgccgtga agtcgtcgcg caatgcctta gagaccgagt
     1741 cgtgccgcag gcggtcgatg atcccggccg ccttgccgag cagctcggac ccccgctcga
     1801 tgatgccctt gctcgcctgc gtgccgtagg tctcctcgta cgagaccgcc cggcgcttgc
     1861 tgtcgcgcac ggtgtcgtgg aaccgctgga tggccgaggt cagcgcggtg ctgtcgctct
     1921 tgagcttgag ctggtggtcc agcgcctcct cgacgtggct ctccagcgcg acaacgtccc
     1981 cgatccagtc ggcgatgacc ttgtgcttct ggtcggtgct catgggctgt gctccttttt
     2041 cctacatgcg ccggtcagcg gcgccgaaac ggcaccatgc tgctaccggg ttccgcagaa
     2101 tccgggccac ggtgcgccag ccgatggtcg cgcaactgcc gggttgctgg gacaatggcc
     2161 ccatcacaac cggcacgccc gtacccatgc ccggagcagc accatgaccg acatggccac
     2221 cgatcgccgc accttcgcaa cccccgtcga tatccgtcac agtccgcacg ccgcgcaccg
     2281 gccggtgccg cccggcaacg tccgcctcga cgatgaga
//
LOCUS       DASYWL010000077        10424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_21793, whole genome shotgun sequence.
ACCESSION   DASYWL010000077 DASYWL010000000
VERSION     DASYWL010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10424
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21793"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VGR22_02855"
     CDS             complement(<1..364)
                     /locus_tag="VGR22_02855"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV2127538.1"
                     /translation="MAPTPTTAPSFGTLLRIYRLAAGLTQEALAERAGLSVRGISDLE
                     RGARRSPYPETVRLLAEGLGFGPDAPERAALLAARGAAEPSEAALERPRLPTPATPLI
                     GRERELAAIAARLARQDIR"
     gene            549..806
                     /locus_tag="VGR22_02860"
     CDS             549..806
                     /locus_tag="VGR22_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127539.1"
                     /translation="MDPNFVAAVLTSAAPQDRDRHPMHLEREMVTYRPARTHPPRSWA
                     VAPRPRIGRLLIATGTHLQGSVLPPGSSPLDNSILGEEAGH"
     gene            1007..1435
                     /locus_tag="VGR22_02865"
     CDS             1007..1435
                     /locus_tag="VGR22_02865"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEV2127540.1"
                     /translation="MTVEQTQQLLDRYLEALFGGGEFGQYLAEDGAIRFMDTGDVVAG
                     REAVVDAIVTSHTVQFAATPQVTNMAVGAGTAGVEIIFAGTHTAEFAGIAATGATVNV
                     PYVAFYTMADNSITEIRLYGLVMGLIAQLSAAGASTTPAG"
     gene            1711..2682
                     /locus_tag="VGR22_02870"
     CDS             1711..2682
                     /locus_tag="VGR22_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEV2127541.1"
                     /translation="MIELSISVEGVVGLTWPEWKRPVRTVEDIGFAGLYLSDHIVLFF
                     EQPDEPSLELVVALTYLADHTERVRFGPMVSPLSVRDPVMLARQAAALDDLSDGRMVL
                     GVGAGWMEREHTMFGYDLGDIPTRFARLEEGLEVITRLLRSDQPANFEGAFFRLHEAV
                     LPEPRRPNRPPVMIGGSGPRRTLPLAARFADVWNATGVTPEQLTERMALLDDLLEVAG
                     RRPEDVRRTLTVPVVCWRSPEELEARVRWARRFPPWRSLTADQLVEEIRTWQAIVGTP
                     EEVASQIRAYAAAGISEIAMQWYASDDIEGLESLAAEVLPQVAAAGA"
     gene            complement(2963..3286)
                     /locus_tag="VGR22_02875"
     CDS             complement(2963..3286)
                     /locus_tag="VGR22_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127542.1"
                     /translation="MMKVTDGLRQIRERLVEQDANRETLKLVDGILKRASLPAAESAT
                     AGSLLQLVRMLMRSPQASSNPAIYNDLVRVEEELEERAQEVRQRVEEEEARPIPKLKK
                     YYKKK"
     gene            3459..4301
                     /gene="thiD"
                     /locus_tag="VGR22_02880"
     CDS             3459..4301
                     /gene="thiD"
                     /locus_tag="VGR22_02880"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV2127543.1"
                     /translation="MHHPATNPPHVMTIGGSDSGAGSGVQADLKTFAALGTYGTSILT
                     AVTAQNTREVAAIAEVPEEIVIAQIDTVAEDIGAQAIKTGMLGNRKLIQNVVDRLEAW
                     GIPHLVVDPVMVSGSGVPLLASDALAPLRDELLPLAAVVTANLDEASALTGIDLTSRS
                     QMEEAARALREMGPSVVVIEGGQMEDLKADLVMNGDTTEWLEAPPAPTPNAHGAGCTL
                     SAAIAALLAHDCEPFEAIRLARLYLTNALEHAYRVGEGLSPVNHFAPLPEEIADRIGQ
                     WTRA"
     gene            complement(4378..4800)
                     /locus_tag="VGR22_02885"
     CDS             complement(4378..4800)
                     /locus_tag="VGR22_02885"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEV2127544.1"
                     /translation="MRVNEKSRARRTSFRRAVLEVVSRIPPGRVTTYGLISRALGFPR
                     GARNVGMALARSSEFGDFPCHRVVNRDGELSGAWAFGHPEVMRQLLEEEGVLFIAEAR
                     VDLDVCLWEPPDLSELLRPPETEPARKYREPNRGEAPR"
     gene            4911..7517
                     /gene="secA"
                     /locus_tag="VGR22_02890"
     CDS             4911..7517
                     /gene="secA"
                     /locus_tag="VGR22_02890"
                     /inference="COORDINATES: protein motif:HMM:NF006630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEV2127545.1"
                     /translation="MRSILKKLFGDGSQREFKDLQVIADDVNALEPDYESATLEHLRE
                     EFQGLGARYRSGEPLDDLLPESFAITREAAKRTVGMRHFDVQIMGGAVLHEGKIAEMR
                     TGEGKTLTATLAVALNALSGDGVHVVTVNDYLAKRDTQWMGQVYHGLGLSVACIQHNA
                     AFMFDPDYEAEDERLENLRPIFRREAYLADITHGTNNEFGFDYLRDNMTVEKDRMVQR
                     ELNYAIVDEVDNILIDEARTPLIISGQAGAATDRYYQFAQIVRQLRKGRDYEVDPKHR
                     NATLTDKGIDTAERLAGIPEGESIYDERYVELTHHLEQALAAQAIYQRDKDYIVRDGE
                     VIIIDEFTGRMMVGRRYSEGLHQAIEAKEGVRVQRQNVTMATITFQNYFRMYKKLAGM
                     TGTAETEQEEFFRIYNLPVMVIPTNKPMIRDDMPDLVFRTENGKFQAVIRDIKKMQEQ
                     GRPVLVGTTSVEKSEILSSLLDKEGIEHQVLNAKQHEREAEIVAQAGEPNAVTIATNM
                     AGRGTDIKLADEVRAAGGLHIVGTERHESRRIDNQLRGRSGRQGDPGSSRFFVSIEDE
                     LMQRFGSDRIGSLMDRLGMAEDEPIEHKIISKSIENAQTKVEGHNFDRRKQVVQYDDV
                     MNRHREYIYRDRRKIVMGGDVSEKVQDLIEAELEAMVDAHTAEKSGDIDYEALVEQFN
                     GLIPGDRVTVEMIAGQESDDIYNILADEAEAAYEALEQEFGEETMRTMERRVLLAVID
                     KLWVDHLTAMDELRAGVGLQAYGQRDPLVVYKTEGYRMFEQLRRNIRHDLVRSIYRVK
                     PVIAQQPVKTRVSEQETVTNAPQEAGDGSSTVRRKGKKVRPNEPCPCGSGKKYKHCHG
                     APTRRGQTSPVA"
     gene            complement(7601..8800)
                     /locus_tag="VGR22_02895"
     CDS             complement(7601..8800)
                     /locus_tag="VGR22_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127546.1"
                     /translation="MDGATDAPGGWPDMEQEGSVFVQTAKIVGIAGVAAGVVGAVLGR
                     VLESQREPSAGERLQAALDDVKGAGGSSFVNQLSEAVEQARKEFERAKKKSKKQRKLV
                     RGKKRGQEEADLASVAASGMLFADYMRRAVRNSSERFGENLQDVLEQTTESLSKNAEQ
                     SRKTSKKRGKELKRDAKKRREAAEKQASEGLEELMKFRKGAADKAEDRLEDIGKFTRA
                     AVEKRVKPALEKAGAVTLAGASKARHRLEEESKDLEKRYADVKPKIEKRYADARPKIE
                     KSAEHYADVAADLVKELRKTAQEQARDAGPLLQDGVEQARELAREGSEQAKERVHEAG
                     DAAKEGSRNAVSLLVWTAIAGGLIYQVLLNEEQKRKVREFAIGAYHEGKAIYQDLRGE
                     NADFSAQ"
     gene            8930..>10424
                     /locus_tag="VGR22_02900"
     CDS             8930..>10424
                     /locus_tag="VGR22_02900"
                     /inference="COORDINATES: protein motif:HMM:NF013314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2127547.1"
                     /translation="MSLPSKSHDVIVIGSGHAGSEAALAAARSGCRTLVITPNLDRTG
                     YMPCNPSIGGPGKSHIVAEIDALGGAMARIADKSAMQVRQLNTSKGPAVRAVRAQCDK
                     TLYAMLMKEELETQERIEMVQDAATCVILADADTAGSARVIGVTTSVSGTIYADAIVV
                     TAGTFLRARMVSGESSIAGGRAGDTSDASLAGSLLDVGINLRRFKTGTPPRIDARTID
                     ISETDRQPGDDEPLWLSRQGMLGEIEPVLLPPAASGIFSQSEIRGGRPQVACFQTSTT
                     DAAHDIIRANLGRAPMYNGSIEGTGPRYCPSIEDKVGRFADKDAHPIFIEPEGWRSHE
                     AYIQGLSTSLPPDVQEAILPHISGLRDARITRYGYAVEYDAVDPTELTHTLESTRVAG
                     LFLAGQVNGTSGYEEAGGQGLVAGTNGAARALGHAPLRLGRNEGYIGVMIDDLVTLPF
                     QEPYRMLTSRAEFRLILRPDTADRRLSDTAASHSLISTDRHADVAAES"
BASE COUNT         1846 a         3571 c         3244 g         1763 t
ORIGIN      
        1 gccggatgtc ttggcgggct agccgcgcgg cgatggccgc gagttcgcgc tcgcggccaa
       61 tgaggggcgt cgccggggtc gggagccgag ggcgctcgag cgcggcttca ctcggctcgg
      121 cggcaccgcg ggcggcgagc agggcggcgc gctcgggggc atcggggccg aagcctaggc
      181 cctcggccag caggcgcacc gtctctggat acggagagcg gcgcgcgcct cgctccaggt
      241 cgctgatccc gcgcacgctg aggcccgccc gctcggcgag ggcctcctgc gtcagcccgg
      301 ccgcgaggcg gtagatgcgc agcagggtgc cgaagctggg ggcggtggtg ggggtggggg
      361 ccatgaccca ttccttggcg gcgatcccga taacccatcg tacacccacg cgatgccccg
      421 gacaagccac tgcgtgggtg ttgcgtgggc atcatgtgtg gtggccagca ggcgacaggc
      481 gcaggatggg agtgtggcgc cggagagcgc cgtgacagcg atcggaacca cgaatggagg
      541 cgatcagcat ggaccccaac ttcgtagccg ccgtcctcac cagcgccgct ccccaggatc
      601 gggaccggca cccgatgcac ctcgagcgcg agatggtcac ctaccggccc gcaaggaccc
      661 acccgcctcg atcgtgggcg gtggcgccac gcccgcggat cgggaggttg ctcatcgcga
      721 cgggcaccca cctgcagggc agcgtcctgc cacctggttc gtccccgctc gacaactcga
      781 tcctcgggga ggaggcgggc cactaacgca agtcttgggc ctggtcagtg gcgtgggggg
      841 gagtcctgaa ttcctcggca attctcgccc acccgaacgc cccaaaccga ttcaactgtc
      901 agttcgcatc tctcatgcaa gatcaccctg tcgaagggac agtgctatgc gtaccaggat
      961 tgccgctcgc ttgggcggtt ttggaccaca cgaaaggagt cagcaaatga ccgttgagca
     1021 gacgcagcaa ctgctggacc gctacctcga agccctgttc ggtggtgggg agttcgggca
     1081 gtacctggcc gaggacggcg ccatccgctt catggacacc ggggacgtgg tggcgggacg
     1141 ggaggccgtc gtcgacgcca tcgtcacctc gcacaccgtg cagttcgcgg ccacgcccca
     1201 ggtgacgaac atggcggtcg gggccggcac cgccggcgtg gagatcatct tcgcggggac
     1261 gcacacggcc gagttcgccg ggatcgcggc aactggggcg acggtgaacg tgccgtatgt
     1321 cgccttctac accatggcgg ataactccat caccgagatt cgcctgtacg gactggtgat
     1381 gggcctcatc gcgcagctct cggccgcagg tgcctccacc acgccggccg ggtagccggg
     1441 tgggcggcgc tggccggatt cacttcgggc cggcactgcg ctgacggcta tctctcgctg
     1501 caccgcgccg ccgtgttcgg gcggtgcagc gactgagctc ctctgatgcg acttcgctgg
     1561 ctctgctcgg cgccagcgac gaaatccgtc atttggccga tgccgcgtcc gcctaggagg
     1621 ggcataatgg gatgaccccg atacacgggg aggcggccgt gcggttggtg ggtcgatctc
     1681 cgagggccat ggcagatgag gaggtgttcg gtgattgagc tatcgatctc tgtcgaagga
     1741 gtggtaggcc tgacctggcc ggaatggaag cgtccggtca gaacggtcga agatatcggc
     1801 ttcgccggcc tttacctctc cgatcacatc gtcctgttct tcgagcagcc cgacgagccg
     1861 tcactggagc tggtggtcgc gctcacctac ctggctgacc acacggagcg agtgcgcttc
     1921 ggcccaatgg tctcgcccct ctccgtccgc gaccccgtga tgctggctcg gcaggccgct
     1981 gccctcgatg acctgagcga tgggcgaatg gttcttggcg ttggtgccgg ctggatggag
     2041 cgcgagcaca cgatgttcgg ctatgacctg ggagacattc ccacgcgctt tgcccgcttg
     2101 gaggaggggt tggaggtcat cacgcggctg ctccggtctg atcaacccgc caacttcgag
     2161 ggagcattct tccgcctgca cgaggcggtc ctccctgagc cccgacgtcc gaatcgccca
     2221 cccgtcatga ttgggggtag cgggccccgg aggaccctgc cgctagccgc gcgcttcgcc
     2281 gacgtctgga atgcgacggg agtcaccccg gagcagctga cggagcgaat ggccctgttg
     2341 gacgatcttc tggaggtggc gggacgccgg ccggaggacg ttcgtcggac gctgaccgtg
     2401 ccggtcgtct gctggcgttc gccggaggag ctggaggctc gggtacgatg ggcgcgccgg
     2461 ttccctccgt ggcgatccct gacagccgac cagctggtgg aggagatccg cacctggcag
     2521 gcgatagtcg gcacccctga agaggtggcc tcgcagatcc gcgcgtatgc tgcggccggc
     2581 atcagcgaga tcgccatgca gtggtatgcg tcggatgaca tcgagggcct ggagtcgctg
     2641 gccgccgagg tgctgcccca ggtggcagcg gcaggggcgt gacccgtcat cggggcacgg
     2701 gatcatgaca gagatgggcc gaaggagcca tgagtgcgac agcgttccca ttcaacattg
     2761 agggcgccgt gcgggagcgg ccgtgacacc cttggacggc cctccacgcc aagacgtgga
     2821 gtccctaccg gtacaggctc tagtcaggca tacccccagc ctccctgtcc tctaggctac
     2881 gcataggcta cgtcgaccac gtgcaggcac accagaaacc caatatctcc agggagacgg
     2941 accacgtcaa ccgctgtttc atctacttct tcttgtagta cttcttcagc ttggggatcg
     3001 gacgcgcttc ctcctcctcg acccgctggc gcacctcctg cgcacgctcc tcgagctcct
     3061 cttcgactct gacaaggtcg ttgtagatcg cagggttgga tgacgcctgc ggcgaccgca
     3121 tcaacatccg caccaactga agcagcgacc ctgccgtcgc tgattccgcc gcgggcaacg
     3181 acgcccgctt gaggatgccg tcgacaagct tcagcgtttc ccgatttgca tcctgctcga
     3241 ccagccgctc acgtatctgt cgcagcccgt ccgtcacctt catcatctgt cctccctccg
     3301 gggccaccgc gggaggtccc ggccgattcg tctgctgccg cttcctatcc taccccacgc
     3361 gttcctcaac tctcggcacc aaccgactca cgagctgtgc cacactaggc cacgaccgga
     3421 cacgagctgc cgcgacggcc attcccgaac ggagacccat gcaccaccct gccaccaacc
     3481 cgccccacgt gatgaccatc ggcggatccg actcgggcgc gggcagcggc gtccaggccg
     3541 acctcaagac tttcgccgcg ctcggaacct acggaaccag catcctcacc gctgtcaccg
     3601 cgcagaacac cagggaggtc gccgccatcg ccgaggtgcc ggaagagatc gtcatcgcac
     3661 agatcgacac cgtcgccgag gacatcggtg cccaggcgat caagaccggc atgctcggca
     3721 accgcaagct catccagaac gtcgtcgatc gcctcgaggc atggggtatc ccgcatctgg
     3781 tggtcgatcc ggtgatggtt tccgggtccg gtgtgccact gctcgcctcc gatgcgctgg
     3841 cgcccctccg cgacgaactg ctgcctcttg ccgccgtcgt aacagcgaac ctggatgaag
     3901 cttcggcgct gaccgggatc gacctgacca gccggtccca gatggaggaa gctgctcgcg
     3961 ccctgcggga gatgggaccg agcgtcgttg tgatcgaggg cggtcagatg gaagacctga
     4021 aggccgatct cgtgatgaac ggagacacca ccgagtggct cgaagcccct cccgccccaa
     4081 cgcccaatgc tcacggtgcc ggctgtacgc tctccgcggc gatcgccgcc cttcttgccc
     4141 acgactgcga gcccttcgaa gcgattcgcc tcgcccgcct ctatctcacc aacgcactgg
     4201 agcatgcgta ccgcgtcggg gaggggctct cccccgtcaa ccatttcgcg ccgctcccgg
     4261 aagagatcgc ggaccgcatc gggcagtgga cgcgcgcctg aaccctgccg gtgcgacgct
     4321 tccaatcgaa tcaccgagcc caccccacgc tggcgcctgt ggcgcaggcg ccagccgtca
     4381 tctcggcgct tcaccacgat tcggctctcg gtacttccga gcgggctctg tctccggtgg
     4441 ccggagcaat tcactgaggt ccggcggctc ccacaggcag acgtcgagat cgacccgcgc
     4501 ttccgcgata aacaacacgc cctcctcttc caggagctgt cgcatgacct ccggatgccc
     4561 gaacgcccag gcaccggaga gttcgccgtc ccggttgacc acacgatggc aggggaaatc
     4621 accgaactcc gacgaccgtg ccagcgccat gccgacatta cgggcgccgc gtggaaaccc
     4681 aagcgctcgg gagattagcc cgtatgtcgt cacccgaccc ggcgggatgc gcgacaccac
     4741 ctccagtacc gcccggcgga acgacgtcct cctcgctctg ctcttctcat tgacgcgcat
     4801 gcaacctgcc cttcagggcg ctgtcacctc gcggtcgtat actggaaggt gcagcgatcg
     4861 tcccgggaca cctgggatcg attcgatttg aatgtggagt catggcaaca atgcgctcaa
     4921 ttctcaagaa actgttcgga gacggctcgc aacgcgagtt caaggatctc caggtcatcg
     4981 ccgacgacgt gaacgcgctc gagcccgatt atgagtcggc cacgcttgag cacctccgcg
     5041 aagagttcca gggtctcggc gcccgatatc gatcgggcga gccgctggat gacctcctgc
     5101 ccgagtcgtt cgcgatcacc cgcgaggccg cgaagcggac ggtcggcatg cgccacttcg
     5161 atgtccagat catgggcggc gcggtgttgc atgagggcaa gatcgccgag atgcgcaccg
     5221 gcgagggcaa gacgctcact gcgacgctgg cggtcgccct caacgccctc tccggcgacg
     5281 gcgtccacgt ggtgacggtg aatgactacc tcgccaagcg cgacacccag tggatgggtc
     5341 aggtctacca cgggctcggc ctaagtgtcg cctgcattca gcacaacgcg gccttcatgt
     5401 tcgatcccga ctacgaggcc gaggatgagc ggctggagaa cctgcggccg atcttccgcc
     5461 gggaagccta cctcgccgac atcacgcacg gcaccaacaa tgagttcggc ttcgactacc
     5521 tgcgtgacaa catgacggtc gagaaggaca ggatggtcca gcgcgaactg aactacgcca
     5581 tcgtcgacga ggtcgacaac atcctcatcg acgaagcccg cacgccgctg atcatctccg
     5641 gacaggcggg cgccgctacc gatcgctact accagttcgc gcagattgtc cgccagctca
     5701 ggaaggggcg cgactacgag gtcgacccca agcaccgcaa cgccacgctg accgacaagg
     5761 ggatcgacac ggccgagcgg ctcgccggca tcccggaggg ggagagcatc tacgacgagc
     5821 ggtacgtcga gctcactcac cacctcgagc aggcgctcgc cgcgcaggca atctaccagc
     5881 gggacaagga ctacatcgtt cgcgatggcg aggtcatcat catcgacgag ttcaccggtc
     5941 gcatgatggt tggtcggcgc tacagtgagg gactccacca ggcgatcgag gccaaggagg
     6001 gcgtgcgagt ccagcgccag aacgtcacca tggcgacgat caccttccag aactacttcc
     6061 ggatgtacaa gaagctcgcg ggcatgacgg gcacggcgga gactgaacag gaagagttct
     6121 tccgcatcta caacctgccg gtgatggtga tcccaactaa caagccgatg atccgcgacg
     6181 acatgccgga tctcgtcttc cgcaccgaga acggcaagtt ccaggcggtg atccgcgaca
     6241 tcaagaagat gcaggagcag ggccgaccgg tgctggtcgg caccacctcg gtcgagaagt
     6301 cggagatact cagctcgttg ctcgacaagg aaggcatcga gcatcaggtt ctcaacgcca
     6361 agcagcacga gcgcgaggcg gagatcgtcg cgcaggcggg tgagcccaac gcggtaacta
     6421 ttgcgactaa catggccggc cgcgggaccg acatcaagct ggccgatgag gttcgcgctg
     6481 ccggtggtct gcacatcgtc ggtaccgaac ggcacgagtc gcgccggatc gacaatcagc
     6541 tccgcggccg ctcagggcgc cagggcgacc ctggatcgtc ccgcttcttc gtctccatcg
     6601 aggacgagct gatgcagcgc ttcggcagtg accgtatcgg cagcctaatg gatcgcctgg
     6661 ggatggcgga ggacgagccg atcgagcaca agatcatctc caagtcgatt gagaacgcac
     6721 aaacgaaggt cgaggggcac aacttcgacc gccgcaagca ggtcgtccag tacgacgacg
     6781 tgatgaatcg ccaccgcgag tacatctacc gcgaccgccg caagatcgtc atgggcggcg
     6841 acgtcagcga gaaagtccag gatctcatcg aggccgagct cgaggcgatg gtcgacgcgc
     6901 acaccgccga gaagtcgggc gacatcgact acgaggcact cgtcgagcag ttcaacgggc
     6961 tgatcccggg cgaccgtgtg acggtcgaga tgatcgccgg tcaggagagc gacgacatct
     7021 acaatatcct cgcggacgaa gccgaggccg cctatgaggc gctcgaacag gagttcggcg
     7081 aggagacgat gcgcacgatg gagcgacgtg tcctgctcgc cgtgatcgac aagctctggg
     7141 tcgaccacct caccgcgatg gacgaattgc gcgccggcgt gggcctccag gcctacgggc
     7201 aacgcgaccc gctggtggtc tacaagaccg aaggctaccg gatgttcgag cagcttcggc
     7261 gcaacatccg ccacgacctc gtccgctcga tctaccgcgt gaagccggtc attgcccagc
     7321 agcctgtgaa gacccgggtc agcgagcagg agacagtcac gaacgctccc caggaggcag
     7381 gcgacggctc gtcgacggtt cgacgcaagg gcaagaaggt gcgcccgaac gagccgtgcc
     7441 cctgcggcag cggcaagaag tacaagcact gccacggcgc accgacccgg cgcggccaaa
     7501 cgagcccggt cgcctgatca gttatccgct atccgcgacg cacgacaacg ccccgtcctg
     7561 acgggccggg gctttgtcac tgcagttcct ggcaacgaat ctactgagcg gagaagtccg
     7621 cgttctcgcc gcgcaggtcc tggtagatcg ccttgccctc gtggtaggca ccgatcgcga
     7681 actcgcgaac cttgcgcttc tgctcctcat tgagcagcac ctgatagatg agcccaccgg
     7741 cgatcgccgt ccagactaga agggagacag cgttcctgct gccctccttg gccgcgtccc
     7801 ccgcctcatg gacgcgctcc ttcgcctgtt ccgacccttc gcgagccagc tcccgtgcct
     7861 gctcgacacc atcctgaagc agcggtccgg catctctggc ctgttcctgc gcggtcttgc
     7921 gcagctcctt gacaaggtcc gcggccacat cggcgtagtg ctccgccgac ttctcgatct
     7981 tcggccgcgc gtcggcgtag cgcttctcga ttttcggctt gacgtcggcg tagcgcttct
     8041 ccagatcctt cgattcctcc tcgagccggt gcctcgcctt gctcgcgccg gcaagcgtga
     8101 cggcaccggc tttctcgagg gcaggcttca ctcgcttctc gacggccgcg cgagtgaact
     8161 tcccgatgtc ctcgagccga tcctcggcct tgtccgctgc acctttccgg aacttcatca
     8221 actcctccag cccttcgctc gcctgcttct ctgcagcttc gcggcgcttc ttcgcgtctc
     8281 tcttcaactc cttgccgcgc ttcttcgagg tcttccgcga ctgctcggca ttcttgctca
     8341 acgactccgt cgtctgctcg agcacgtcct gcaggttctc gccgaatcgc tcggaactat
     8401 tccgtacggc gcggcgcatg taatcggcga acagcatccc cgatgcggcc actgaggcaa
     8461 gatccgcttc ctcttgcccc cgcttcttgc ccctcaccag cttgcgctgc ttcttgctct
     8521 tctttttcgc gcgttcgaac tccttgcgcg cctgctccac cgcctcgctc aactgattga
     8581 cgaacgatga accgcccgcc cccttcacgt catcgagtgc agcctgcagc cgctcgccgg
     8641 cgcttggctc ccgctggctc tccagcactc gccccagcac cgcaccgacg acaccggcag
     8701 cgaccccggc aatgcctacg atcttcgcgg tttgcacgaa gaccgatccc tcttgctcca
     8761 tatcaggcca ccctcccggc gcgtccgtag ctccgtccat acgtctttcc tgaaccttgt
     8821 cttatgataa tcgttgctct gggaatacgg cgcagagccc atatgcgcga actagcacac
     8881 tttgtgccaa ctggattctg ccgtagctgc acccgagacc ccacctgcca tgtccctccc
     8941 ctccaagtcc catgatgtca tcgtgatcgg ttccggccac gccggctcgg aggctgcgct
     9001 cgccgccgca agatccgggt gtcgcacgct ggtcatcacg cccaacctcg accggaccgg
     9061 ctacatgccg tgcaatccct ccatcggtgg gcccggcaag agccatatcg tggccgagat
     9121 cgacgcgctt ggaggcgcga tggcgcggat cgcggacaag tccgccatgc aggtacggca
     9181 gctcaacacc agcaaggggc ccgccgtgcg ggcagtacgg gcccaatgcg acaagacgct
     9241 ctatgcgatg ctgatgaagg aggaactcga gacacaggag cggatcgaga tggtgcagga
     9301 tgctgccacc tgcgtgatcc ttgccgatgc cgatacagca ggatctgcac gcgtcatcgg
     9361 cgttaccacc agcgtctccg gtaccatcta cgccgacgcc atcgtcgtca ccgccggaac
     9421 cttcctccgg gcgcgcatgg tctcgggcga gagcagtatc gccggtggtc gcgccggcga
     9481 tacctctgat gcctccctcg ccggctcgct cctcgatgtc ggcatcaacc tgcgacgctt
     9541 caagacgggc accccgcctc gcatcgacgc cagaacaatc gacatctccg agaccgatcg
     9601 gcagccgggc gacgacgagc cgctctggct cagccggcag ggcatgctcg gcgagatcga
     9661 acctgtgctc ctgccaccgg ccgcgtccgg catcttcagt caatccgaga ttcgaggcgg
     9721 gcggccacag gttgcctgtt tccagacatc gacaaccgac gccgcccatg acatcatccg
     9781 cgccaatcta ggccgggcgc cgatgtacaa cggctcgatc gagggcaccg gcccgcgcta
     9841 ctgcccgtct attgaggaca aggtcggccg cttcgcggac aaagacgccc acccaatctt
     9901 tattgagcca gagggctggc gatcgcatga ggcctatatc caggggctca gcacgagcct
     9961 tccccccgac gtgcaggagg cgatcctgcc gcacatctcg ggactccgtg acgcgcgcat
    10021 cacccggtat gggtacgccg tcgagtacga cgcagtcgat cccaccgagc tgacccatac
    10081 gctggagagc acccgagtgg cggggctctt cctcgcggga caggtcaacg gcacctcagg
    10141 ctacgaggag gccggcggcc aggggctggt ggccgggaca aacggggccg ctcgcgcgct
    10201 cggccacgcg ccgttgcggc tcggccggaa tgagggctac atcggcgtga tgatcgacga
    10261 cctcgtcacc ctgccctttc aagagcccta ccggatgctg acctcgcggg ccgagtttcg
    10321 gctcatcctc cgtccagaca ccgccgaccg gcgtctcagc gacaccgccg ccagccacag
    10381 cctcatctcg acggatcggc atgccgacgt tgctgccgag tctg
//
LOCUS       DASYWL010000078         4601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_509881, whole genome shotgun sequence.
ACCESSION   DASYWL010000078 DASYWL010000000
VERSION     DASYWL010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509881"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..130
                     /locus_tag="VGR22_02905"
     CDS             <1..130
                     /locus_tag="VGR22_02905"
                     /inference="COORDINATES: protein motif:HMM:NF020672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HEV2127548.1"
                     /translation="YRDLFSTSRKYAQPTLEYLDEQRVTRRKGDVRVRFRGPGAAT"
     gene            668..1312
                     /locus_tag="VGR22_02910"
     CDS             668..1312
                     /locus_tag="VGR22_02910"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEV2127549.1"
                     /translation="MSVASGFGLLVYGMTIGVALAAPIGPINIEIIRRGIQNGYLQGW
                     LVGLGALSADTVYAALIVSGLTPLADSPALRVPLFLAGAVMLSWVGINSLRTALRGTG
                     LQQGQQPVGRSSYLAGLLMAVFNPMGIVYWLSVGAALVADAVERVGAAGAPLLVGGVF
                     VGIFLWVTFLSWLAQVARRFVNGRGMLWITGIGGVALIGFGLWFLYQAASILFG"
     gene            1360..3132
                     /locus_tag="VGR22_02915"
     CDS             1360..3132
                     /locus_tag="VGR22_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2127550.1"
                     /translation="MQQSRGATTCGVSRRGVVASGISIAGSVALGRVAAQTPTAGPQA
                     SPVASPVASPVLNHPLDIVHAGELPEDGQPVRGGTLRLPVHPVGLTNLVLPLHGQDPQ
                     VAWSYLDALVRIDRETGLPAPSLARAWTWSEDGHTLSFELRDDVRWHDGRPFTAEDVR
                     FSHIVYRDDFRSIFAAQTALVDDVVVVDDIVVEVRFAEPDGAWLFNVASLPILPAHQY
                     REAWEANPVGERTLEGVSFGDELLVGTGPWVIESISASGVAFSRNEDYFAGPPHADRL
                     ELLAVERMDERVALWREGDLHAVGHLDPQRVEGLLDDEGRLVVADSPRSLFAALNFNN
                     PDRVNPTVLVDMALRDAINLAIDRERYAEEIWNGFLRHERPGLIMLPWVETGDEKNSS
                     RNLARARKVLEEAGFGDYNGDGVLDSPAGDALVLTTIVQDTVEPAVVDTLEAMDADLC
                     EIGIALSIEVLPPEAFVARWSVDRTWDLIVYDLVLYPAFASFDLVGSAWDIAANAAGW
                     NPGGYWNPAVDEALAAYFAAVDEEEMADALADLQQGLIDDPFAIWLGQPQTLTLLAPG
                     VRGFTPNPLWLTLDTRLMWLAEDE"
     gene            complement(3455..4294)
                     /locus_tag="VGR22_02920"
     CDS             complement(3455..4294)
                     /locus_tag="VGR22_02920"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEV2127551.1"
                     /translation="MDLPAAIQETLETRVARTLGDTVVRSRTLTGGKMGDVVRIDLQR
                     VGPVVAKSTSPDARPTLEAAMLRELRASETIPVPDVLHAEDDLLVLEFIDGMHLRPEA
                     ECHCDALLAQLHDVHGKAFGFATETLNGRIMLASPWTESWVAFYREHRLQLSLALVDR
                     YSPLPPDMHADLDRVLSQNDRLLREPERPSLLHGDLWAANVLSSGDRVTAFLDPSACY
                     GDPEIELAYVDAWVSFGYHFGDRFIPRLAATGSSGMPAPHTDRSRMASTRSASTSTHS
                     TRC"
     gene            4382..>4601
                     /locus_tag="VGR22_02925"
     CDS             4382..>4601
                     /locus_tag="VGR22_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEV2127552.1"
                     /translation="MVADPVILAIDQGTTSSRAVIFDRRGRPLGSAQQETRQQYRQPG
                     WVEQDASEIIKVTFAMARQVLAETGIGVE"
BASE COUNT          780 a         1394 c         1594 g          833 t
ORIGIN      
        1 gtaccgcgat cttttcagca ccagtcgcaa gtatgcccag ccgacgttgg agtacctcga
       61 tgagcagcgt gtgacgaggc gcaaggggga tgtacgcgtt cggtttcgcg ggccgggggc
      121 ggctacctga tctaacgccg aggggtcggc cgagatcagg gcgactggcc ctcgggagcc
      181 tacgttcacc cttgacacag agagtatgat gggagtactg aagcatcggg acgctgccga
      241 acacctgaat actcaaccat tgagcaggat tccaaacgtt cggcggctcc gtgggtggct
      301 ggaagtactc ggtgtgaccc accggtgcat ccttttccag tctttccagg aacccggtcg
      361 gaagtcagct ccggcgaccg atgggcgttc ggaacatggg tcgaatggca ccacttcgac
      421 acagccgaac gtgatcgcgc gcgaagcgcg atctggacgg ggttctggca gcggaggtgc
      481 aaccagccga tgaggctgcc agcctggaac gtctgccttt cccagggcgg cgacttgtca
      541 gacaaaggga tgcttcagtc tgaatgaagg caccggcccc gtaccacggg ccggtgctct
      601 ttgtttcagt ccatggcccg tgtcgtacac tccaatcgct aatgggatgc gtcgagacag
      661 gagaagcatg agcgtcgcaa gcggctttgg tttgctggtg tacggcatga cgataggtgt
      721 cgcgctggct gcgccgatcg gtccgatcaa tatcgagatc atcagacgcg ggatccagaa
      781 cggctatctg caggggtggc tggtcggcct gggcgcgctg tcggcggata ccgtctacgc
      841 ggcgctgatc gtcagcggcc tgacaccgct ggcggacagc cccgcgctgc gggtgccgct
      901 cttcctggcc ggtgcggtga tgctctcctg ggtcggtatc aacagtcttc gcacggcatt
      961 gcgaggtacc gggctgcaac agggtcagca accagtcggt cgaagcagct acctcgcggg
     1021 tctgctgatg gcggtcttca acccgatggg tattgtctac tggctttcgg tcggcgccgc
     1081 gctggtcgcg gatgccgtcg agcgggtcgg ggcggccggc gcgccattgc tggtcggcgg
     1141 ggtgttcgtc gggatctttc tctgggtcac cttcctctca tggttggccc aggttgcccg
     1201 acggttcgtg aacggtcgcg gcatgctctg gatcaccggc atcggcggtg tggcgctgat
     1261 cggcttcggg ctctggttcc tctaccaggc ggcaagcata ctatttggat aggacgggcg
     1321 ttgcagagac gcgggggcgg gatggaggcg gagcttcaca tgcagcagtc acgaggcgcg
     1381 acgacgtgcg gtgtttccag acgaggcgtc gttgcgagtg ggatcagcat cgccggttca
     1441 gtcgcgctcg ggcgtgtcgc cgcccagacg ccgaccgccg gtccgcaggc gtcaccagtc
     1501 gcctcgccgg ttgcgtcacc ggtgctcaac catccgctgg acatcgtcca cgccggcgag
     1561 ctgccggaag acgggcagcc ggtgcggggc ggtacacttc ggcttcccgt gcaccctgtt
     1621 ggcttgacga acctggtgct gccactacac gggcaggacc cgcaggttgc gtggagctac
     1681 ctcgacgcac tcgttcgcat cgatcgcgaa accggtctcc cggcgccatc gttggcacga
     1741 gcatggacgt ggagcgagga cggccacacg ctctccttcg agctgcgcga cgacgttcgc
     1801 tggcacgatg gcagaccctt cacggccgag gacgtgcggt tcagccacat cgtctaccgg
     1861 gacgatttca ggagcatctt tgccgcccag accgcgctgg tcgacgatgt tgttgtggtc
     1921 gacgacatag tcgtggaggt gcgattcgcc gagccggacg gcgcatggct ctttaacgtt
     1981 gccagcctgc cgattcttcc tgctcaccag taccgcgagg cctgggaggc gaaccccgtc
     2041 ggcgagcgaa cgcttgaagg tgtttccttc ggtgacgaac ttctggtcgg cacgggtcca
     2101 tgggtgatcg agtccatctc cgcgagcggc gtggccttct cccgcaatga ggactatttc
     2161 gcggggccgc cacacgcgga ccggctggag ctgctcgccg tcgagagaat ggacgagcgg
     2221 gtggcactct ggcgcgaagg cgatctgcac gccgttggcc atctcgatcc ccagcgcgtc
     2281 gaggggctgc tggatgacga ggggaggctt gtcgttgccg atagcccgcg ctcgctcttt
     2341 gcagcactca acttcaacaa tccggaccga gtcaatccaa ccgtgttggt ggacatggcg
     2401 ctgcgggatg ccatcaatct cgcgatcgac cgggagcgct atgccgagga gatctggaac
     2461 gggttcctgc gccacgagcg accggggctg atcatgctcc cgtgggtcga gaccggcgac
     2521 gagaagaact catcgcgaaa cctcgctcgc gcccggaagg tgctcgagga ggccggcttt
     2581 ggcgactaca acggcgacgg cgttctcgac agtccggccg gcgatgcgct cgtgctgaca
     2641 acgatcgtgc aagacacggt cgagccggcg gtggtcgaca cattggaggc gatggacgcc
     2701 gacctgtgcg agatcgggat cgcgctcagc atcgaggtcc tgccgccgga ggcctttgtc
     2761 gcgcgctgga gcgttgaccg cacctgggac ctgatcgtct acgacctcgt actctacccg
     2821 gcgttcgcgt cgttcgatct cgtcggctcg gcatgggaca tcgccgccaa cgcggccggc
     2881 tggaatcctg gtggctactg gaacccagcg gtcgacgagg cgctggctgc ctacttcgca
     2941 gcggtcgacg aggaggagat ggcggatgcg ctcgccgatc tccagcaggg gctgatcgac
     3001 gacccgttcg cgatctggct cggtcagccg cagacgctca ccctgctggc tcctggtgtt
     3061 cgcggattca cgcccaaccc cctctggctt accctcgaca cgcggctcat gtggctggct
     3121 gaggacgagt gatcgctgtg tgggtggccc atcagctgga ggggacgact tggctgtgct
     3181 ccgcaacgca cgaggcaggc cccgtgagca cgtgcaatag cctgccctga gcgagggccg
     3241 caggcccgag tcgaacgggt cgtcccgctt ccagccacgc gtttccaaca cgatgggtca
     3301 acaatggttg cgtgggagga cattgaccgc gcggacgaag agttccatga ctccggtgtg
     3361 cgcggtcaag tcctccccta cacgtgcggc ttgatggcgg cgatggtggc agcgaggcgg
     3421 gggatgaacc ggtcgccgaa gtaatagacg tgcatcagca gcgggtagag tgcgtagacg
     3481 tgcttgcgga cctggtggaa gccatcctcg atcgatcggt gtgcggtgca ggcatcccag
     3541 aactcccggt ggcagcgagg cgggggatga accggtcgcc gaagtgatag ccgaacgaaa
     3601 cccaagcgtc aacgtatgcc agctcgatct ccgggtcgcc atagcaggct gaggggtcga
     3661 ggaacgctgt gacgcgatcg ccgctggaga gcacattggc ggcccacagg tcaccatgca
     3721 ggagcgatgg ccgttccggc tcgcgcagca ggcgatcatt ttgggacagg acccggtcga
     3781 ggtcggcgtg catgtcaggc ggcagcgggg agtagcggtc caccaacgcc agggagagct
     3841 ggagccggtg ctcccggtaa aaggcgaccc acgactcggt ccagggactc gccagcatga
     3901 tccggccgtt gagcgtctct gttgcgaagc cgaacgcctt gccgtgcaca tcatgcaact
     3961 gcgcaagcag cgcatcgcaa tggcactccg cctctggccg caggtgcatt ccgtcgatga
     4021 attccagcac cagcaggtcg tcctcggcgt ggagcacatc gggtacggga atcgtctcgc
     4081 tcgctcgcag ctcgcgcagc atcgcggctt cgagcgtggg acgtgcatcg gggctcgtcg
     4141 acttcgcgac caccggaccg acgcgttgga gatcgattcg caccacatcg cccatcttgc
     4201 caccggtgag cgttcgcgac cgtacgacgg tgtcacccag cgtgcgggcg acgcgggtct
     4261 cgagcgtctc ctgaatggct gcggggaggt ccatggggcc gtcgtcaggt ggcgacgtgc
     4321 cagatgtcga taggctagca tgttcctgcg acgccgatgc agaaggggga gggaagtggc
     4381 catggtagct gatccagtga ttctcgcgat cgaccaggga acgacgtcga gccgtgccgt
     4441 gatcttcgac cggcgggggc gcccgcttgg ctctgcccag caggagacga ggcagcagta
     4501 ccggcagccc gggtgggtcg agcaggacgc cagcgagatc atcaaggtca cctttgcgat
     4561 ggcgcgccaa gtgttggcgg agaccggcat cggcgtcgag c
//
LOCUS       DASYWL010000079        10850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_416437, whole genome shotgun sequence.
ACCESSION   DASYWL010000079 DASYWL010000000
VERSION     DASYWL010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10850
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416437"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VGR22_02930"
     CDS             complement(<1..371)
                     /locus_tag="VGR22_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV2127553.1"
                     /translation="MYRRATLKDVAEVAGVSTATVARVLHNKGYVAPGTRRLVEQALA
                     DTGYQINAIAQGLRRQRTYTIGHVMNSVIPNQFFAAVAEGVEEVSSRHGCGVLMVTTH
                     GDSTRERRSVETLIQRRVDAI"
     gene            685..1461
                     /locus_tag="VGR22_02935"
     CDS             685..1461
                     /locus_tag="VGR22_02935"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV2127554.1"
                     /translation="MFRLDGKTALVTGAGSGIGREIALLFAKQGATVAVGDINDEGAA
                     GVVDEIAGVGGSAVALHLDVSSLDSANQAVAGIVTQTGRLDILVNNAGIGMVGSLTET
                     EPSDFDRLMAVNVNGVYYCSQAAVNQMLQQDPQGGVLVNIASIAGQVAVARRFAYGTA
                     KGAVISMTQSIAMDYIDRAIRCNCICPGTVESPFVEAYLQRYHTGEIEETRRQLHARQ
                     PLGRMGRPDEIAPLALYLASDESAYVTGSQMVIDGGLTAR"
     gene            1488..3083
                     /locus_tag="VGR22_02940"
     CDS             1488..3083
                     /locus_tag="VGR22_02940"
                     /inference="COORDINATES: protein motif:HMM:NF045169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HEV2127555.1"
                     /translation="MAQISNLSNLQGARGRVPLTDLAVLLHPDDNVIIAKSPLLPKTT
                     LMRDDGDDIVIGRMVPPGHKVANHHVAKGQPVRRYGQIIGFATADIAPGEHVHNHNVA
                     VHQGELELDYAFCEDVKPVDFVPEGKRRTFMGYRRKDGRVGTRNYVAVIATVNCSSTA
                     TRRVADHFRMHPEIMERFPNVDGVIPFPTKGGCGAHYGSPDLGMLQRTMANVVDHPNV
                     AGYVMLSLGCEVNQPDDLIEATHLSLNGKKPLVLTIQEGGGFQKTVEAGITAVMDMLP
                     EANKAQREPVPASELMVALQCGGSDGWSGVTANPALGKAADEIVRQGGTVVLGETSEV
                     YGGEHLLTRRAATREAGEKLVERIHWWEEYTSMFGASIDNNPSPGNKLGGLTTIYEKS
                     LGAIAKAGSTPLNDVVPYAEKVTAKGFVHMDTPGYDPVSVTGQVAGGCNVVTFTTGRG
                     SAFGFKPAPSVKLATNSDLYHKQEPDMDIDTGKVLEGVSLDEMGQEIFEHILRVASGE
                     RSKSELADIGEEEFNPWIIGAML"
     gene            3292..3861
                     /locus_tag="VGR22_02945"
     CDS             3292..3861
                     /locus_tag="VGR22_02945"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HEV2127556.1"
                     /translation="MTETSLAPTDALYRQLEAALAKVGGVCHRAADIPFAAEAIENIM
                     GGSDVPLWTAPMVRDALPALLDTLEGAGVRLRYPTGAVEVRDQAFGLSVARQAIAETG
                     SVVLVERELTDRAVGLVTETHIVICPLDALAPSLDDAGETLAAVAQEHGSYATLVTGP
                     SRTADIERQLTVGVQGPARLHLIVIDTPA"
     gene            3876..6113
                     /locus_tag="VGR22_02950"
     CDS             3876..6113
                     /locus_tag="VGR22_02950"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HEV2127557.1"
                     /translation="MSSTAKRSTTPPHATHAEAPFEERYDTAMANERLAVSLTNFQQS
                     WRRDRANVMAEVEFEQLQRTMKAAKTDAIDNLDTYLDQFIAAAELSGTHVHLAADADE
                     ANRIIEEIARAHSVEVVAKSKSMVSEETELNDHLTGLGMEVVETDLGEWIVQLRNERP
                     SHMVMPAIHLAREQVGDIFSEHLQREISREDVAEQVSAARDAIRDVFFKAGMGITGAN
                     ALIAESGTVMMCTNEGNGRLTSSIPPVHVVLAGIEKLVPTYADAMRQLRLLARSATAQ
                     RITVYSTFIQGPTPGHEQHVVLVDNGRRAMREREEFREALHCIRCAACANVCPPYREV
                     GGHVFGYIYSGAIGLVVTPFLHGLDAGAGPQSLCLSCNACETVCPVGIPLPRQILDVR
                     AMVAAGKGIRVTKRIALNVYARPRAADLLMKIGSRAQLPLTRGSRLIRAGRLPGLKRQ
                     TSWRSLPALARRPLHERVRERCERPERPGPSPPLIPSAVAGMRVALFPGCMTDRLYPE
                     QGEAIATVLGALGAEMVHPEGLHCCGLVHQNSGDVDHARDLGRRTIASLERVEADWII
                     SGSASCVAMIIQDYAHQFRDDPAWRERAERLARRVMDFTSFLVRIAKLQKGTLAAPDG
                     ASRTVTYHDSCQSLNALGLSAEPRYLIERVMGDEIAELPENTLCCGFGGTFSVDYPDV
                     AKRLMTRKLTNAEATGAHVIVTDNQGCIMHLRGGSDAGSRGLEVRHLAELLAERVEQR
                     RRADT"
     gene            6391..7266
                     /locus_tag="VGR22_02955"
     CDS             6391..7266
                     /locus_tag="VGR22_02955"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEV2127558.1"
                     /translation="MPLCRFHTGDPADAHIGFLDGEQVIAFPELSGLATMREVLRLTR
                     TDLGTAIARIRNAAESAIAAHDATLLAPIDEQEVWACGVTYLRSRDARMEESTEQDVY
                     ERVYNADRPELFFKATPSRVSGPGQPVGIRADSRWDVPEPEVALVINAYGEVIGYTVG
                     NDMSSRSIEGENPLYLPQAKMYTACAGLGPVIALADEITDPRDLTVKLTIERGGTVLF
                     EEETSTDQMARSFEDLVGYLHRHNTFPDGVFLMTGTGIIPPSEFTLEDDDLVSIAVEG
                     IGALAQPVTRLSTTG"
     gene            complement(7329..7493)
                     /locus_tag="VGR22_02960"
     CDS             complement(7329..7493)
                     /locus_tag="VGR22_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127559.1"
                     /translation="MSPAVKATLYGELTTSVILLIALLISAPNATTLLLAVAFGLVVA
                     AATWYQGRCR"
     gene            complement(7490..7654)
                     /locus_tag="VGR22_02965"
     CDS             complement(7490..7654)
                     /locus_tag="VGR22_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127560.1"
                     /translation="MNNRSNSMVIMGIVTLIAVLAVLLLLGVEFWLATVIAVVCALAA
                     QLITGRQARR"
     gene            7682..8260
                     /gene="moaC"
                     /locus_tag="VGR22_02970"
     CDS             7682..8260
                     /gene="moaC"
                     /locus_tag="VGR22_02970"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015428892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEV2127561.1"
                     /translation="MLTGHSLETGIIGQLPIVIVIDSDMDINDTPQGKQGLTHFDERG
                     NARMVDVGHKPETERVAVATGRISMTPETAEVIREGRAKKGDVLGVARVAAIMAAKKT
                     ADAIPMCHPIPITRVDIGFELDETGVDIEARVDTKGQTGVEMEALHAVSVAALTIYDM
                     VKAIDRGMTIERIRLEYKEGGQAGIWQREEQP"
     gene            8510..9400
                     /gene="sdhC"
                     /locus_tag="VGR22_02975"
     CDS             8510..9400
                     /gene="sdhC"
                     /locus_tag="VGR22_02975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEV2127562.1"
                     /translation="MASISSEQGRFRFGLAGVGGNDRLAWYFLRISGIALVFFALGHM
                     FITHYLNAPSETDFDFVAARWSNTLWLTFDSTLLIAALWHGLVGFRFILADYVPRGGR
                     VLAYGALWLIGLIFTVLGLVNIFTFDVEAARNNAGPLAGEMWIGDVLGYSLYAFAVVT
                     YIAVIALVIWVLRNMQQGVATIYNGDAGQYAWVLHRATGAGILFFLLVHILDIMLVTL
                     GRDVYDHTVEFYAEPFIIPMEILLVGAVVYHTLNGLRVIAVNFSSRGPAREKKYFWAV
                     LALSVLLTIPSVIVIFSAEF"
     gene            complement(9518..10081)
                     /locus_tag="VGR22_02980"
     CDS             complement(9518..10081)
                     /locus_tag="VGR22_02980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV2127563.1"
                     /translation="MSERRIEVAAIVGSLRRNSYNRGLLRTAMLLRPDRIHLYEVPIH
                     DLPYYNEDYERRGFPAVVGEYRAQLDRADALLICTPEYAYSIPGVLKNALDWTVRPVQ
                     QSPVRGKAMAIMGASMGRSGTMRAQLHLRQLAVPLGMLPVPEPEVYITFAADKFNQLG
                     ELTDHTSRELVAEMMENLADWTELLRG"
     gene            complement(10264..>10850)
                     /locus_tag="VGR22_02985"
     CDS             complement(10264..>10850)
                     /locus_tag="VGR22_02985"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642545.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEV2127564.1"
                     /translation="RAQFNSYGSRRGNHEVMIRGTFANIRLRNQLTPGKEGNWTKYHP
                     TGEEMSIYEASEKYMADDTDLVVIAGKEYGTGSSRDWAAKGTLLQGVKAVIAESFERI
                     HRSNLVGMGVLPLQFMKGESAESLGLTGEETFSIRDLDANLSPNATLTVDVTRADGST
                     SSFDARARVDSNVEVMYYRNGGILPAVLRRLLAA"
BASE COUNT         1968 a         3652 c         3416 g         1814 t
ORIGIN      
        1 atgatcgcgt ccacccgtcg ctggatgagt gtctcgaccg atcgcctctc gcgagtcgag
       61 tcgccatggg ttgtaaccat gagcacacca cagccgtggc gggatgacac ctcctcgact
      121 ccttcggcga cggcagcgaa gaactggttt gggatgacgc tgttcatgac atgaccaatc
      181 gtgtaggtcc gctgtcggcg tagcccttgg gcaatggcgt tgatctggta accggtatcc
      241 gcgagtgcct gctcgactag gcgccgggtg cccggcgcga cgtagccctt gttgtgaagg
      301 acgcgggcaa cagtggcggt tgatacacca gctacttccg caacatcctt gagcgttgca
      361 cgacggtaca tccgatggtc tcctacggga tctgacgccg tgtgtcatcg atgacacata
      421 cggtatcagg gcttacgggc cgtgtcaatc cctcattaca acgcgacaca tattaccaag
      481 caggtaggtg cggcgctcaa aacgagcacc gacatgaacg cctactggtc catgtttaag
      541 atcggcattc acagttcggc gaattttgta tacaatcgct agatcggagg gacagatgat
      601 ctccgctatc gatgccccgc ccggctcgtc gtcaccatgc cgtcgtcgcg ctctccctcg
      661 ctcggcgacg aggaggagcc caagttgttc agactcgatg gaaagactgc cctcgtcacc
      721 ggcgccggat ccggcatcgg ccgggagatc gcgctcctct tcgcgaagca gggtgcgacg
      781 gtcgccgtgg gcgatatcaa cgacgagggg gcggccggcg tcgtcgacga gattgccggt
      841 gtgggcggca gcgccgtcgc gctccacctc gacgtcagca gcctcgattc ggcgaaccag
      901 gcggtcgccg gcatcgtgac gcagaccggc cgactcgata tcctcgtcaa caacgccggc
      961 atcggcatgg tcggcagcct gaccgagacg gagccgtccg acttcgaccg cctcatggcg
     1021 gtcaacgtga atggcgtcta ctactgctcc caagcggcgg tgaaccaaat gctccagcag
     1081 gacccgcagg gcggcgtgct ggtcaacatc gcctccatcg ccggacaggt cgccgttgcg
     1141 cgacgattcg cgtacggtac cgccaagggc gcggttatct cgatgaccca gtcgattgcg
     1201 atggactata tcgaccgggc gatacggtgc aactgcatct gcccgggcac cgtggagtct
     1261 cccttcgtcg aggcctacct gcagaggtat cacactggcg agatcgagga gacgcgccgg
     1321 caactccacg cccgccagcc gctcggccgc atgggccggc cggacgagat cgccccgctt
     1381 gccctctacc ttgcctcgga cgagtccgcc tatgtcaccg gatcgcagat ggtgatcgac
     1441 ggcggcctga ccgcgcgata gcccgattcc cgaaacggag ccccgccatg gcacagattt
     1501 ccaatctctc caacctgcaa ggcgcgcgtg gacgcgtccc gcttacggac ttggccgtcc
     1561 tgctgcaccc cgacgacaac gtgatcatcg ccaagagccc gctgctgccg aagacaacgc
     1621 tcatgcgcga tgacggtgac gacatcgtga tcggtcggat ggtcccgccg gggcacaagg
     1681 ttgccaacca ccacgtcgcc aagggacagc cggtgcggcg ctacgggcag atcatcggct
     1741 tcgcgacggc cgatattgcc cccggcgagc atgtccacaa ccacaacgtc gccgtccacc
     1801 aaggcgagct ggaactggac tacgccttct gtgaggacgt gaagcccgtc gatttcgtac
     1861 cggagggcaa gcgccgcacc tttatgggct accggcgcaa ggacggtcgt gtcggcacgc
     1921 gcaactacgt cgcggtgatc gcgacagtca actgctcgtc gacggcgacg cgccgggtgg
     1981 ccgatcactt ccggatgcat ccggagatca tggaacggtt cccgaacgtt gacggcgtca
     2041 tcccctttcc gaccaagggc ggctgcggcg cgcactacgg ctccccagac ctcggcatgc
     2101 tccaacgcac catggcgaac gtcgtcgacc acccgaacgt cgcgggctac gtgatgctca
     2161 gcctcggctg cgaggtcaac cagccggacg acctcatcga ggcgacccac ctctccctca
     2221 atgggaagaa gccgctcgtg ctgacgatcc aggagggcgg tgggttccag aagacggtcg
     2281 aggcaggtat caccgcggtg atggacatgc tgccggaggc gaacaaggcg cagcgcgagc
     2341 cggtcccggc atccgagctg atggtcgcat tgcagtgcgg cggctcggac ggatggtcgg
     2401 gcgttaccgc gaacccggca ctcggcaagg ccgccgacga gatcgtccgg cagggcggca
     2461 ccgttgtgct cggcgagacg agcgaggtgt acggcggcga acacctgctc acccggcgtg
     2521 cggcgacccg tgaggctggc gagaagctgg tcgagcgcat ccactggtgg gaggagtaca
     2581 cctccatgtt cggtgcatcg atcgacaaca acccctcacc gggcaacaag ctgggcggcc
     2641 tgacgacgat ctatgagaag tcgctcggcg cgatcgccaa ggccggttcg accccattga
     2701 atgacgtcgt tccttacgcc gagaaggtca ccgcgaaggg cttcgtacac atggacacgc
     2761 cgggctacga cccggtgtcg gtgaccggcc aggtcgcggg tggctgcaac gtggtgacgt
     2821 tcacgaccgg gcgcggttcg gcattcggat tcaagccggc gccgagcgtc aagctcgcga
     2881 ccaacagtga cctctaccat aagcaggagc ccgacatgga catcgatacc ggcaaggtgc
     2941 ttgagggtgt gagcctcgac gagatgggcc aggagatctt cgagcacatc ctgcgggtgg
     3001 cgtccggcga gcggagcaag agcgaactgg ccgacatcgg cgaagaggag ttcaaccctt
     3061 ggatcatcgg cgcgatgctg taagccgtgc cctcgccgtc aaccaacttg tcacttcgac
     3121 cggagcacac aaagtatgcg gagaggagaa atccctcctt cgtctgatcg cgactatcga
     3181 atctcaagga ccataccggg agggattcct ccacgcgggc ctgtcggccc ttggtcgaaa
     3241 tgacagagtg gctatagcgt tcgttgagtc tcgaagcccc aggaccgcac catgaccgag
     3301 acatccctcg cgccaactga cgccctctac cggcaactcg aagcggcgct ggcgaaggtc
     3361 ggtggagtgt gccatcgcgc cgccgacatt ccgttcgccg ccgaggcgat cgagaacatc
     3421 atgggcggca gcgacgtgcc gctctggacc gcaccgatgg tccgcgacgc cctcccagcc
     3481 ctcctcgaca cgctcgaggg ggccggcgtc aggctccgct atcccactgg cgcggtagag
     3541 gtgcgcgacc aggccttcgg gctgagcgtg gcgcggcagg cgatcgccga gaccggctcc
     3601 gtcgtgctgg tcgagcgcga gctgaccgac cgcgccgtcg ggctggtaac cgagacccat
     3661 atcgtcatct gcccgcttga cgccctcgcg ccctcgctcg acgatgcggg cgagaccctt
     3721 gccgctgtgg cccaggagca cggctcctac gcgacgctgg taaccggccc gagccgcacg
     3781 gccgacatcg agcggcagtt gaccgttggc gtacagggac cggctcgact gcatttgatc
     3841 gtcatcgaca cacccgcctg atccccggag cctgcatgtc ttccaccgcc aagcgcagca
     3901 ccaccccgcc gcacgccacc cacgccgaag ccccgttcga ggagcgatac gacacggcga
     3961 tggccaacga gcgcctcgcc gtcagcctca ccaacttcca gcagtcgtgg cggcgggacc
     4021 gtgccaacgt gatggccgaa gtcgagttcg agcaactcca gcggaccatg aaggctgcca
     4081 agaccgacgc catcgacaac ctcgacacct acctcgacca gttcatcgct gctgctgagc
     4141 tatccggcac ccacgtccac ctcgccgccg acgccgacga ggcgaaccgg atcatcgagg
     4201 agatcgcgcg ggcacacagc gtcgaggtcg tcgccaagtc gaaatccatg gtcagcgagg
     4261 agaccgagct caacgaccat ctcaccggcc tcggcatgga ggtggtcgaa accgacctcg
     4321 gcgagtggat cgtgcagctg cggaacgagc gcccgagcca catggtgatg ccggcgatcc
     4381 acctggctcg tgaacaggtc ggcgatatct tcagcgagca cttgcagcgc gaaatctccc
     4441 gcgaggacgt tgccgagcag gtcagtgcgg cgcgggatgc catacgtgat gtcttcttca
     4501 aggccggcat gggcatcacc ggagcgaatg cactcatcgc agagtccggt accgtgatga
     4561 tgtgcaccaa cgagggcaac gggcggctca cctcgtccat cccgccagtc catgtggtgc
     4621 tggccggcat cgagaagctg gtccccacct acgccgacgc gatgcggcag ctccggctgc
     4681 tcgcccgcag cgcgacggcc cagcgcatca cggtctactc aaccttcatt cagggtccga
     4741 cgccggggca cgagcagcac gtcgtgctgg tcgacaatgg ccgccgcgcg atgcgcgagc
     4801 gcgaggagtt ccgcgaggcg ctgcactgca ttcgctgcgc cgcgtgcgcg aacgtctgcc
     4861 cgccctaccg tgaggtcggc gggcacgtct tcggctacat ctactccggc gcgatcggcc
     4921 tagtcgtgac gccgttcctg cacggactcg acgccggcgc cggcccgcag agcctctgcc
     4981 tctcctgcaa cgcctgtgag acagtctgcc ccgtcggcat ccccctgccc cgccagattc
     5041 tcgacgtgcg ggcgatggtc gccgccggca agggcatcag ggtcaccaag cgcatcgcgc
     5101 tcaacgtgta cgcccgccca cgcgcggcgg acctgctgat gaagatcgga tcccgcgcgc
     5161 agctgcccct cacgcgcggg tcccgattga tccgtgcggg tcgtctgccg ggcctgaagc
     5221 gccagacgag ctggcgcagt ctgcccgcgc ttgcccggcg tccgctccac gagcgcgtgc
     5281 gggaacgatg tgagcggccc gaaagacccg ggccatcgcc gccactgatc cccagcgcgg
     5341 tggcgggcat gcgggtcgcc ctcttccccg gctgcatgac cgaccggctc taccccgagc
     5401 agggcgaggc aattgcgacc gtgctcggtg ccctcggtgc cgagatggtc cacccggagg
     5461 gcctgcactg ctgcgggctg gtgcaccaga actcgggcga tgtcgaccac gcccgcgacc
     5521 tcggccggcg gacgatcgcc tcgctggagc gcgtggaggc cgactggatc atctccggca
     5581 gcgcgagctg cgtggcaatg atcatccagg actacgcaca ccagttccgc gacgacccgg
     5641 cctggcgcga gcgcgccgag cggcttgcca ggcgcgtgat ggatttcacg tcgttcctcg
     5701 tgcggattgc gaagctgcag aagggcacgc tggccgcgcc ggacggtgcc tctcgcaccg
     5761 tcacctacca tgactcctgc caaagcctga acgcactcgg gctctccgcg gagccgcgct
     5821 acctgatcga gcgggtgatg ggtgacgaga tcgccgaact gccggaaaac acgctctgct
     5881 gtgggttcgg tggcacgttc agcgtcgact atcccgacgt cgcgaagcgc ctgatgaccc
     5941 gcaagctgac caacgccgag gcaaccggcg cacacgtgat cgtcaccgat aaccaaggct
     6001 gcattatgca tctgcggggc ggcagcgacg cggggagtcg cgggctcgaa gtccggcacc
     6061 tcgccgagct gctcgccgag cgggtggagc agcgacgccg ggcagacacg tagcaatcag
     6121 ctgcagtttc cttctcgcgc gccaacctaa cctgtcattt cgagccggca aggcccgtta
     6181 gcgccggcgg ccgtgtcgag aaatctcttg cccgccatga ggtggcatct ggctcgcgag
     6241 agatttcccc actccggacc ctccgcgtcc tgcggtcggc atgacaccga tatcaacaac
     6301 accaagcggc tcgacacccc gtcgatcccc acatacgatg aagcgcactt gccatcagaa
     6361 catggttcac cacgaaagga gccaaatcct atgcccctct gccgtttcca caccggcgac
     6421 cccgccgacg cccacatcgg gttcctcgac ggcgagcagg tcatcgcctt cccggagctg
     6481 tccgggctcg ccaccatgcg cgaggtgcta cgcctcacgc ggaccgacct cggcaccgcc
     6541 atcgcccgca tccgcaatgc ggccgagtcc gcgatcgccg cgcatgacgc cacgctgctc
     6601 gccccgatcg acgagcagga ggtctgggcc tgcggcgtca cctatctgcg gagccgcgac
     6661 gctcggatgg aggaatcgac cgagcaggac gtctacgagc gggtctacaa cgccgatcgc
     6721 ccggagctct tcttcaaggc gacaccctca cgcgtttcgg gtccggggca gccggtggga
     6781 atccgcgccg attcgaggtg ggacgtgccc gagccggaag tcgccttggt gatcaacgcc
     6841 tacggcgagg tgattggcta cacagtcggc aacgacatgt cgtcacggtc gatcgagggc
     6901 gagaacccgc tctacctgcc gcaggcgaag atgtacacgg cctgcgccgg gctcggaccg
     6961 gtgatcgcgc tcgcggacga aatcaccgac ccgcgcgatc tcacggtgaa gctgacgatc
     7021 gaacgcggcg gcacggtgct gttcgaggag gagacctcga ccgatcagat ggcgcggtcc
     7081 ttcgaggacc tcgtcggcta cctgcaccgg cacaatacct tcccggacgg cgtcttcctg
     7141 atgaccggca cggggattat cccgccgtcc gagttcacgc tggaggacga cgacctggtg
     7201 agcatcgccg tcgagggcat cggcgcgctc gcccagccgg tgacacgact ctccaccact
     7261 gggtgatgcg catgaccgcg gcgagatgaa gccggacaga cgattcgcag ggcgacatta
     7321 cacggcgatc accggcaacg cccctgatac caggtcgcgg ccgcgacgac gagcccgaag
     7381 gcgaccgcca gcagcaatgt ggttgcgttc ggtgccgaga tcagcagcgc aatcagcagg
     7441 atcacgctcg tggtgagctc accgtagagc gtcgccttga cggccggact catcgcctgg
     7501 cttggcgccc ggtgatcaac tgcgccgcca gcgcgcacac caccgcgatc accgtcgcca
     7561 gccagaactc gacaccaagc agcagcagca ccgccagcac tgcgatcagc gtgacgattc
     7621 ccatgatgac catcgagttc gatcggttgt tcatggtcgt tcctctcccg ccatcggcgg
     7681 catgctgacc ggtcactctc tcgaaaccgg tatcatcgga caactgccaa tcgtcatcgt
     7741 gatcgacagc gacatggaca tcaacgatac cccacagggc aaacagggac tcacccattt
     7801 cgatgagcgc ggcaatgccc gcatggtcga tgtcgggcac aagccggaga ccgagcgcgt
     7861 tgccgtcgcc accggtcgca taagcatgac accggaaacg gccgaggtaa tccgcgaggg
     7921 acgtgcgaag aagggcgacg tgctcggtgt ggcgcgcgtg gccgcgatca tggctgccaa
     7981 gaagacagcc gacgccatcc ccatgtgcca cccgattcct atcacgcgcg tcgacatcgg
     8041 cttcgagctc gacgagacgg gcgtcgacat cgaggcgcgt gtcgacacga agggacaaac
     8101 cggtgtcgag atggaggcgc tccacgccgt cagtgtggcc gccttgacca tctacgacat
     8161 ggtcaaggcg atcgaccgcg gcatgacaat cgagcgcatt cgccttgagt acaaggaagg
     8221 tgggcaagcc gggatctggc agcgcgagga acagccatga tcccggcttg cactcgcaat
     8281 cctgctctgc tggctcgatg ggtgctgcag tggccgtacc caactaggca tgaccacacc
     8341 gttcgccccg gcgcgaacag gaccagccgc gtacaatagg gacaaccatt gacggagggt
     8401 ccggagttca ggatcatgcc ggtgccacga ctcgctggcg ggcacgacct gaatcgttgt
     8461 gccaatccgc acaacgacac caatgaccac gggggaggat tagtaaggca tggcctcgat
     8521 ttcgagtgaa cagggccggt tccggttcgg tttggccggc gtcggcggca acgaccggct
     8581 tgcgtggtac ttcctgcgca tctccggcat tgccctggtc ttcttcgccc tcgggcacat
     8641 gttcatcacc cactacctga acgcaccctc ggagaccgac ttcgacttcg tcgccgctcg
     8701 gtggtccaac acgctgtggc tgacgttcga cagcaccctc ctgatcgccg cgctctggca
     8761 cggcctggtc ggcttccgct tcattctcgc cgactacgtt cctcgcgggg gtcgcgttct
     8821 cgcctacggc gccctctggc tgatcgggct gatcttcacg gtgctcggac tcgtcaacat
     8881 cttcaccttc gacgtggagg cggcccgcaa caacgccggc cccctcgccg gcgagatgtg
     8941 gatcggcgat gtgctgggct actcgctcta cgccttcgcc gtcgtcacct acatcgcggt
     9001 gatcgcgctc gtcatctggg tccttcgcaa tatgcagcag ggggtcgcga cgatctacaa
     9061 cggtgacgcc ggccagtacg cctgggtgct gcaccgggcg accggcgccg gaatcctctt
     9121 cttcctcctg gtgcatatcc tggacatcat gctggtcacg ctcgggcgag atgtctacga
     9181 tcacaccgtc gagttctacg ccgagccgtt catcatcccg atggagatcc tgctggtcgg
     9241 cgcggtggtc tatcacacgc tgaacgggct tcgcgtgatc gcggtgaact tctcgtcgcg
     9301 cggtccggcc cgcgagaaga agtacttttg ggcggtgctc gcgctgtcgg tgctgctgac
     9361 catcccgagc gtgatcgtga tcttcagcgc ggagttctag acgctggaag tactacacgt
     9421 gcagccccgg cctgaatggt gcccggagtc tttgcatcca gtgcgggtgg acgacgtgcc
     9481 tgtactgcgg gcggcaatgc ctggactcgc agggggatca gccgcgaaga agctcggtcc
     9541 agtcggcaag gttctccatc atctctgcga ccagctcgcg tgaggtatgg tcggtcagct
     9601 caccgagctg gttgaacttg tcggcggcga aggtgatgta gacctccggc tcgggcaccg
     9661 gcagcatccc gagcggaacc gccagctgcc gcaggtgcag ttgcgcacgc atcgtaccgg
     9721 atcgccccat ggatgccccc atgatcgcca tcgccttgcc gcgcacgggc gactgctgta
     9781 ccggtcggac cgtccagtcc agcgcgttct tgagcacgcc agggatcgaa taggcgtact
     9841 ccggagtgca gatcaacagc gcgtcggccc ggtcgagttg tgcgcggtat tcccccacca
     9901 cggcaggaaa gccgcgccgc tcgtagtcct cgttgtaata tggcaggtcg tggatcggca
     9961 cctcgtagag gtggatccgg tcggggcgaa gcaacattgc ggttcggagc aggccccggt
    10021 tgtacgaatt gcgacggaga ctgccgacga tcgcagccac ctcgatgcgt cgttcggaca
    10081 cgaacgcttt ccccaatccg ccgtcgctgt gcggtacacg atgtgatgga cggcgccagc
    10141 gtccgtcctc ggtctcgata attctagcat cagccgtccg gatagcccca tgccccgaac
    10201 cggtcagcga caccggagcc ggttacgatc gcgaccggcc ccgagtcgag tgtcaggtat
    10261 ccatcaggcg gccagcaggc gccgcagcac tgccggaagg atgccgccgt tgcggtagta
    10321 catcacctcg acattgctgt cgacccgggc acgagcgtcg aagctggagg tcgagccgtc
    10381 cgcgcgggtg acatcgaccg tcagcgtcgc gttcgggctc aggttggcgt ccagatcccg
    10441 aatcgagaac gtttcctcgc cggtcagccc gaggctctcg gcgctctcgc ccttcatgaa
    10501 ctgcagcggc agcacgccca tgccgacgag attcgaacgg tggatgcgct cgaagctctc
    10561 ggcaatgacc gccttcactc cctgcagcaa tgtccccttc gccgcccaat cgcgtgagga
    10621 gccggtaccg tactccttgc ctgcgatcac caccagatcg gtgtcatccg ccatgtactt
    10681 ctcggacgcc tcgtagatcg acatttcctc gccggtggga tggtacttcg tccagttccc
    10741 ctccttgccc ggcgtcagct gattgcgcag acgaatattg gcgaaggtgc cgcgaatcat
    10801 cacctcatga ttgccgcgcc gcgagccgta ggagttgaac tgcgcccgct
//
LOCUS       DASYWL010000080         9327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_228648, whole genome shotgun sequence.
ACCESSION   DASYWL010000080 DASYWL010000000
VERSION     DASYWL010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9327
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228648"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1265)
                     /locus_tag="VGR22_02990"
     CDS             complement(<1..1265)
                     /locus_tag="VGR22_02990"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HEV2127565.1"
                     /translation="MARRDVWYKNAIIYCLDVETYADSNGDGVGDFPGLTSRLDYLAG
                     LGVNTLWLMPFYPSPGLDDGYDITDYTSVDPRYGSMADFVDFMVEARELGMRVIIDLV
                     PNHTSNQHPWFQAAREDPSSKYRDYYVWRQDEPPDTSDQVVFPGVQESIWTYDEKVQA
                     WYLHHFHAFQPDLNYANPQVQREFEKIMGLWLELGVAGFRVDAAPFLINLTGVDTGVF
                     DMEMAHQYLEAMRDFVVRRRGNAILLGEVDVGLSTISDFFGGGNQLQALFNFPMNRYV
                     FLGLAQESADPLSFGLDQLPTIPDDGQWVNFLRHHDELNLARITHAQRETIYEAFGSE
                     AKYQVFGRGLRRRLAPMLDGDLDRIKLAFSLLFSLPGAPMIFYGEEIGMGENLKLERR
                     MAVRAPMQWISSDGGGFSSAPMDEYVRPIL"
     gene            complement(1319..5572)
                     /locus_tag="VGR22_02995"
     CDS             complement(1319..5572)
                     /locus_tag="VGR22_02995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HEV2127566.1"
                     /translation="MTMVVTGIRWPTTLASYFDSATSPLNPYNAGFGAYVYGTFPLFL
                     AKAIGFITGDLNYGDAHLPGRWLSALADTGTVMLAFWIARRLFSTAAGLLAALLTALA
                     PLHIQSAHYFTTDSTATFFAVASFAAVLRSTSGQRWRWMILAGICVGLATASKPNLAI
                     TAGFLLIPLLENVRTGGPAALGPRGGWRRALALSPAIRLLVAGVVAAVTFRIAQPYAF
                     TGPHIWNISLDPRWTDTLDYWRQIQRGSIDAPPGVQWADRTPLVFILDNLVRWGMGPA
                     LGIAALIALTWFGIRMLSERRWPSWWLLAVILWAAFHIAVYGAGLVQAQRYLLPAYPF
                     LIVLAVGWLVTLPVRVREIGWRPSPIVLARLRGALIALVAFGTVVSGVAMTSVYLRGH
                     TRVAASEWIYEHVPPGSVIATEHWDDGLPLGLPGQPVDAYRFAQIPGYDADTEIKLSR
                     LIGRLQQADYVVLSSDRLIETVPRIPDRYPMMRAYYEALQSGELGFELVQSFTSPPTF
                     LGIEFDDRGAEEAFTVYDHPQVRIFRKTDAWSAHAAWQLLDDALGEGGVPRRVIDPST
                     SQLMLTEAEQREVVEGGTWSEIVDAPSMPTALVVLLWYLALQLAAVPAVLALGRLLSM
                     LPDRGYAVAKLIGLLAIAWGVWLLASLRLVPFGRVSIGAVFVVMLLLALVATWRRWGD
                     ALRELRERWRWVVGTEVLFLIAFGGMLWMRAMNPDLWQPGRGGEKPMEMAIFTAVMRS
                     EWFPPYDPWFAEGVLHYYYLGYMPWVALTRVLGITPEIAFNLALPSLFALLVVSVWSA
                     AAALLVASRRRWDIPVSGWRPILLALAAPVLVVLVGNLDFVRRLGRGEWGGEPPPEWA
                     GSLGEVGLVLVGIWRALTVSPELPQDAYWAPSRVIPNTVNEFPYFTFLFGDLHAHVLG
                     MPVVAATVVIAIALATGAMRDTGGADVLATLGGWRQALPLAVLGGFVSGTLLGTNPWD
                     HPPALTLLVAGAAIHLLVSAGFAQPWRSVRDLAIFVATLLVVGRVLYWPFLDRYGALS
                     PEILPAVESTALRDYLTIHGLFLAAVAGYLLVTVSWSAALLWRRGRAGRAMAAVAVLL
                     LIALYVGAFVWGDTRLFLIAGLATSLLAMVGAARLPAHLLIITMTGLALALALSAEVV
                     RFSNDIGRMNLVFKFYLHAWQVLGIAAAASIVLVIAGGGRLRSRRVPGGFLAAMRGVW
                     LMIVAVLLAGALAYPALATATRLGDRIAPISPTLDGLAYMQHAEFTEGPSGGEPSVVS
                     AASDRAAIAWLREQVQGTPVILEAQLPAYRWGGRISSTTGLPTVLGWTWHEIQQRPGH
                     GPIVDQRVSDVATIYGSPHGLEAVEPLLERYGVKLIIVGDLERALYPASALDKFSEAA
                     EEGQLDVVYEANGVTIYAMPGYQGPSAGAKGLMAASSLR"
     gene            complement(5794..6594)
                     /locus_tag="VGR22_03000"
     CDS             complement(5794..6594)
                     /locus_tag="VGR22_03000"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2127567.1"
                     /translation="MTDRRCDSTDYLTRHLQEMAPHRALLRSVEARLMGRVPLVHPVL
                     DVGCGDGHFASVAYREPIDVGLDPMTRDLREAARRVPEVYLEVVQGSATSMPFEDASF
                     ATVVSNCVIEHIPDVDATLRELSRVLRPGGTFATTLPSEHYPDYLLGSTLARKFGVVR
                     VTDAYGAFFNRISHHYHVDPPEVWQRRFEAVGLDVAEHHYYFSPEAHRAFDLAHYIGV
                     PNLITKRLLGRWVVHPTQARPLERWYRRYYEEPLPETGAYQFVRCVRR"
     gene            complement(6591..7778)
                     /locus_tag="VGR22_03005"
     CDS             complement(6591..7778)
                     /locus_tag="VGR22_03005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV2127568.1"
                     /translation="MRVLLGVTYYRPHVSGLTIYAERLARALVAHGHQVTVLTSRYSS
                     SLSVNEVVDGVHIVRVPVALRVSKGVIMPTLGVRAARLMREHDVLSLHLPMFDAAGLT
                     VRARRGGTPVTLTYHCDLQLPPGPFNRVVGLTVHLANRVAARLADRIVAYTQDYADHS
                     RLLRQFPGKLVVIPPPVVMPSPTPEDVSAFRETHGLDDSPVIGMAGRLAAEKGVEHLV
                     AAMPMLSERLPNLKVLFAGPHREVIGEERYRAWLMPKIEALGDRWRFLGTLSPEALPA
                     FYGTLDALLLTSTNSTESFGLVQVESMLCGTPVVATDLPGVRQPVRTTGMGEIVPPGD
                     SEALASAVLAVLDNPGRYGRSREEIEQAFDIEVTIAGYERLFAELLAERREASTAAVE
                     GQG"
     gene            complement(7788..8828)
                     /locus_tag="VGR22_03010"
     CDS             complement(7788..8828)
                     /locus_tag="VGR22_03010"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEV2127569.1"
                     /translation="MQERRSVLVTGGAGFIGSNVVDHALAAGHHVTVFDDLSRPHVDH
                     NIDWLRERHGDAFDMLVASLTDADACERAVQDVDVIYHLAGQTAVTRSVADPRRDFEV
                     NALGTLNVLEAGRLTGRRPIVLYSSTNKVYGAMEGVATVELATRHAYRDLPNGVDESQ
                     PLDFHSPYGCSKGAGDQYVRDYARIYDLPTVVFRQSCIYGPRQMGVEDQGWLAWFVIA
                     LATDQPITIYGDGKQVRDVLYIDDLLKLYDAAVERIDVAAGQIYNVGGGPKNAISIWW
                     ELKPLAEEVLGRRLADPCFDESRPGDQAVFIADIGKARRELGWEPSVAPQDGIRRLTE
                     WITANRDRLSAV"
     gene            9025..>9327
                     /locus_tag="VGR22_03015"
     CDS             9025..>9327
                     /locus_tag="VGR22_03015"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEV2127570.1"
                     /translation="MQILVTGGAGFIGSHLVDQLLAGGHHVTIADSLLTGHRANIAHL
                     RGERRLRFVLQDIADPLAPELAETPFDWIYNLASPASPRGYTRYPLETIHANSAGVW"
BASE COUNT         1802 a         3090 c         2975 g         1460 t
ORIGIN      
        1 agaatcgggc ggacatactc gtccatcggc gccgacgaaa agccgcctcc gtcggatgat
       61 atccactgca ttggcgcgcg caccgccatg cgccgctcaa gcttcagatt ctcccccatc
      121 ccgatctcct cgccgtagaa gatcatcggc gcgcccggca gactgaacaa caggctgaag
      181 gccagcttga tccgatcgag gtcaccgtcc agcattggtg cgagccgccg gcgaagaccg
      241 cgcccgaaga cctggtactt cgcttcggaa ccgaaggctt cgtaaatggt ctcccgctgc
      301 gcgtgcgtga tccgggccag gttgagctca tcgtggtggc gcaggaagtt gacccactgg
      361 ccgtcgtccg ggatcgtcgg gagttggtcc aagccgaagc tcaacgggtc cgcgctctcc
      421 tgtgcgaggc caaggaaaac ataccggttc atcgggaagt tgaagagcgc ctgcagctgg
      481 ttgccgcctc cgaagaaatc cgagatggta ctcaatccga catcgacctc gccgagcagg
      541 atcgcgttgc ccctgcgcct caccacgaag tctctcatcg cctccagata ctgatgggcc
      601 atctccatgt cgaagacgcc cgtgtccacg ccggtgagat tgatcaggaa cggcgcggcg
      661 tcaacccgga agccggcgac gccgagctcg agccaaagac ccatgatttt ttcgaactcg
      721 cgctggacct ggggattcgc gtagttcagg tctggctgaa atgcatggaa gtgatgcagg
      781 taccacgcct gcaccttctc gtcgtacgtc cagatactct cctgaacacc agggaacacc
      841 acctggtcgc tggtgtcggg aggctcgtct tgccgccaga cgtagtagtc acggtatttc
      901 gacgacggat cctcgcgagc cgcctgaaac cacggatgct ggttagaggt gtggttgggg
      961 acgaggtcga tgatgacccg catccccagc tccctcgcct cgaccataaa atcgacgaaa
     1021 tcggccatcg agccgtagcg ggggtcgaca gaggtgtaat cggtgatgtc gtacccatcg
     1081 tcgagtcccg ggctcggata gaacggcatc agccacagcg tgttgacgcc gagccccgcc
     1141 aggtaatcca ggcggctggt cagcccaggg aagtccccaa caccgtcacc gttcgaatca
     1201 gcgtaggttt ccacgtccag gcaatagatg atcgcattct tgtaccagac gtctcggcgg
     1261 gccattgctc ctcctgtgca tcgtcgaagg cgatgcctgt ggcgggtctc ctggctcctc
     1321 accgcaggga agatgccgcc atcaaaccct tagcgccagc tgatggacct tggtagccag
     1381 gcatcgcgta gatcgtgacg ccgttcgcct cgtacacgac gtctagttga ccttcctccg
     1441 cggcctcgga aaacttgtcg agcgcggacg ccggatagag cgcgcgctcg aggtcgccca
     1501 cgatgatcag cttaaccccg tagcgttcca gcagcggctc caccgcctcg agcccgtggg
     1561 ggctgccata gatggttgca acatcgctga cgcgctggtc gacgatcggt ccgtgcccgg
     1621 gtcgctgctg gatctcgtgc caggtccacc caagcaccgt cggcagaccg gttgtgctgc
     1681 tgattcgccc tccccagcgg tacgcgggaa gctgggcctc caggattacc ggtgtgccct
     1741 gtacctgttc ccgcagccac gcgatcgccg ctcggtcact ggcggcagag acaaccgatg
     1801 gctcacctcc gctcggcccc tcggtgaact ccgcgtgctg catgtacgcg aggccgtcca
     1861 gtgtcggcga gatcggcgcg atgcggtcgc cgaggcgcgt tgcggttgcg agcgccgggt
     1921 aggccagtgc accggccagc agcacggcga cgatcatgag ccacacgccg cgcatcgcgg
     1981 caaggaagcc acctggaact cgacgtgagc gcaggcgccc gcctccggca atcaccagga
     2041 cgatggatgc cgccgccgca atgcccagca cctgccaggc gtgcaggtag aacttgaaga
     2101 cgaggttcat gcgaccgatg tcgttgctga accgcaccac ctcggctgat agcgccaacg
     2161 ccagtgccag gccggtcatc gtgatgatga gcaggtgcgc gggcagccgg gcggctccga
     2221 ccatcgcgag gagcgatgtc gcgagaccag cgatgaggaa caacctggtg tcaccccaaa
     2281 cgaacgcgcc aacatagagc gcgatgagca gcaagaccgc cactgctgcc atagcacgcc
     2341 ctgctcgtcc gcgccgccag aggagcgctg ccgaccacga caccgtcacc aagagatagc
     2401 ccgcgaccgc agcgaggaac aacccatgga tggtcagata gtcgcggagc gccgtcgact
     2461 cgaccgccgg caagatttcc ggcgacagcg cgccgtagcg gtcgagaaac ggccagtaga
     2521 gcactcggcc gacgaccagc agggtggcca cgaagatggc gaggtcgcgt accgatcgcc
     2581 atggctgcgc gaagcccgct gacacgagca gatggatcgc cgcaccggcg acaaggagtg
     2641 tcaacgccgg cgggtgatcc cacgggtttg tgccgagcag cgtcccgctg acaaagcccc
     2701 caagcaccgc gagcgggagc gcttggcgcc acccgccgag cgtggccaaa acgtcggcgc
     2761 cacctgtgtc gcgcatcgcg ccggtcgcga gcgcgatggc gatgactact gtggcggcca
     2821 cgaccggcat tcccaacacg tgggcgtgca agtccccgaa cagaaacgtg aagtacggaa
     2881 actcgttcac agtgttcgga atcacccgac tcggcgccca gtaagcgtcc tggggcagct
     2941 ccggcgagac cgtgagcgcg cgccagatgc ccaccagcac cagcccgacc tcgcccaacg
     3001 agccggccca ctccggcggc ggttccccgc cccactcacc tcgtcctagt cggcgcacga
     3061 agtcgagatt gccgaccagt acgaccagca ccggagccgc aagcgcaagc agaatcggtc
     3121 gccagccgct gaccggtatg tcccatcggc gccgtgaggc gacgagtagc gctgcagccg
     3181 cgctccagac actgacgaca agcagcgcga aaagcgacgg gagcgccagg ttgaaggcga
     3241 tttccggtgt gatgccgaga acccgggtga gcgccaccca cggcatgtag ccgaggtagt
     3301 agtagtgcag cacgccttcg gcaaaccatg ggtcgtacgg cgggaaccac tcgctgcgca
     3361 tgacggcggt gaagatcgcc atctccattg gcttctcgcc accccgaccc ggctgccaaa
     3421 gatcgggatt catcgcccgc atccagagca tgccaccgaa cgcgatcagg aagagcacct
     3481 cggtgccgac cacccagcgc catcgctcgc gcagctcgcg cagggcgtca ccccaccgcc
     3541 tccacgttgc caccagcgcg agcaggagca taacgacaaa gacggcgccg atggagaccc
     3601 gaccgaaggg caccaggcgc aggctggcaa gcagccagac cccccaagcg atggcgagca
     3661 gtccgatcag cttcgcgacg gcatagccac ggtccggcag catcgacagc aatcggccca
     3721 gcgcgagcac cgccggtacg gcagccagct gcagcgccag gtaccacagc agcaccacca
     3781 gcgccgttgg catgctcggc gcgtcgacga tttccgacca ggtaccgccc tcgacgacct
     3841 cgcgctgttc ggcctcggtc agcatcagct gcgaggtcga cgggtcgatc acccggcgcg
     3901 gcactccgcc ctcgcccagc gcgtcgtcga gcagttgcca ggccgcatgc gccgaccatg
     3961 cgtccgtctt cctgaagatt cgcacctgcg gatggtcgta gaccgtaaac gcctcctcgg
     4021 cgcctcggtc gtcgaattcg attcccagga aagtaggcgg cgatgtgaac gattggacca
     4081 gctcgaagcc gagctcgccg ctctggagcg cctcgtagta ggctctcatc atcgggtatc
     4141 ggtcgggaat gcgcggaacc gtctcgatga gcctgtcgct cgagagcacg acgtagtccg
     4201 cctgctgaag ccgaccgatg aggcgtgaga gtttgatctc ggtatcggcg tcatagccgg
     4261 ggatctgcgc gaaccggtag gcatcgaccg gctggccggg cagccccagc gggagcccgt
     4321 cgtcccagtg ctcggtggcg atcacactgc ccggaggcac atgctcgtag atccactccg
     4381 aggcggcgac gcgagtgtgc ccgcgcaggt agaccgatgt catcgcaacg ccggagacca
     4441 ccgtaccgaa cgccaccagc gcgatcagtg cgccacggag ccgggcgagc acgattggcg
     4501 agggtcgcca gccgatctcg cgcacgcgca cgggcagggt gacgagccag cccaccgcca
     4561 gcacgatcag gaacgggtaa gccggcagca ggtagcgctg cgcctgcacg agtccggcac
     4621 catagaccgc gatgtggaac gctgcccaga gaattaccgc cagcagccac caactcggcc
     4681 aacggcgctc cgacaacatc cgaatgccga accaggtcag cgcgatcagg gcggcgatgc
     4741 cgagggcagg gcccatcccc caccgcacca gattgtcgag gatgaagacg agcggcgtgc
     4801 ggtcggccca ttggaccccg ggcggtgcgt cgatcgaacc tcgctggatc tgacgccagt
     4861 agtcgagcgt gtcggtccag cgggggtcga gcgagatgtt ccagatgtgc gggccggtga
     4921 aggcgtaagg ctgggcgatc cgaaaggtca ccgcagcgac gacaccggcg accagcagcc
     4981 ggatggctgg cgagagcgcc agcgcccttc tccagccacc tcggggtccg agcgccgccg
     5041 ggcccccggt ccggacattc tccagcagcg ggatgagcag aaacccggca gtgatcgcga
     5101 ggttaggctt gctcgcggta gccaacccga cgcagatgcc ggcgagtatc atccagcgcc
     5161 atcgctgccc actggtcgat cgcaacaccg ccgcaaacga tgcaaccgcg aagaacgtcg
     5221 ctgtggaatc ggtcgtgaag tagtgcgccg actggatgtg cagcggcgcc agggcggtga
     5281 gcagcgccgc gagcagtccg gcggctgtcg aaaagaggcg ccgcgcgatc caaaaggcga
     5341 gcatgacggt accggtgtca gcgagagccg acagccagcg tcccggcaag tgcgcgtcgc
     5401 catagttcag gtcgccggtg atgaagccga tcgccttggc gagaaagagc gggaacgtcc
     5461 cgtagacgta ggcgccgaaa cctgcattgt aggggttgag cggcgaggtg gcgctgtcga
     5521 agtagctcgc cagcgtggtc ggccagcgaa tgccggtgac caccatggtc atgaaccgct
     5581 cgtcgggatg aagataatga ttcgcccccc aggtcgaccc tgcgaagcgc aggactgcgg
     5641 ccaggaccag gatcagcccc agcgcaagcc aggaccgata gcgcagcggg ctgacccgga
     5701 ccggttcacg ccgaggaaca tcgacgggct gccgccgggc gcatcttggc ggaacctcac
     5761 ggtgtccggc gaccggtatc ggccgggttc gcctcacctc ctgacgcatc gcacgaactg
     5821 atacgcgccg gtctcgggca gtggctcctc gtagtatcgc cggtaccagc gctccagcgg
     5881 tcttgcctgc gttgggtgga ccacccaacg accaaggagc cgcttcgtga tgagattggg
     5941 cacgccgatg tagtgcgcca gatcgaatgc gcggtgtgcc tcgggcgaga agtagtaatg
     6001 gtgctcggcc acatcgagtc ccaccgcttc gaagcgtcgc tgccagacct ccggcggatc
     6061 gacatgatag tggtgcgaga tgcgattgaa aaaagcgcca tacgcatctg tgacgcggac
     6121 gacgccaaac ttgcgggcga gcgtcgatcc gagcagatag tcgggatagt gctcgctcgg
     6181 aagtgtcgtc gcgaaggtgc ctcccggacg gagcacgcgg ctgagctcgc ggagtgtggc
     6241 atcgacatcg gggatgtgct cgatcacgca gttggaaacc acggtggcga acgaggcgtc
     6301 ctcgaacggc atgctggtcg cactgccctg cacgacctcc agatagacct cggggacccg
     6361 gcgagctgcc tcccgtaggt ctcgagtcat gggatcgaga ccgacgtcga tcggctctcg
     6421 gtaggccacg gacgcgaagt gcccgtcgcc gcagccaacg tccaggacag gatgcacgag
     6481 cggcaccctg cccatcaggc gggcctccac cgagcgcagg agcgcccgat gcggagccat
     6541 ctcctgcaga tgccgggtga ggtagtcggt gctgtcacag cgacggtcgg tcatccctgc
     6601 ccctccaccg ctgccgtcga cgcttcccgc cgctcggcaa gcagctccgc gaagaggcgc
     6661 tcgtatccgg ctatcgtcac ctcgatatcg aacgcttgct cgatctcctc tcgtgatcgc
     6721 ccgtagcggc cggggttgtc gagcacggcg agcactgcac tcgccagtgc ctcggaatcg
     6781 cccggtggca cgatctcgcc catgcccgtt gtccgtaccg gctgtcgaac tccgggcaag
     6841 tccgtcgcga cgaccggcgt gccgcagagc atggattcga cctgcacgag cccgaacgac
     6901 tccgtcgagt tcgtgctagt gagcagcaag gcatcgagcg tgccgtagaa ggccgggagg
     6961 gcttccggag aaagcgtgcc gagaaaccgc catcgatccc cgagcgcctc gatcttcggc
     7021 atcagccagg cacggtatcg ttcctcccca atgacctccc gatgcgggcc agcgaacagc
     7081 accttcaggt tcggtagccg ctcgctcagc atcggcatgg ctgccaccag gtgctcgacc
     7141 cctttctccg ccgcaagccg ccccgccatg ccgatcaccg gcgaatcgtc aaggccatgc
     7201 gtctctcgga aggccgacac gtcctcaggg gtcggggacg gcatcaccac cggtggtggg
     7261 atcaccacca gcttgcccgg gaactgccgg agcaggcggg agtggtccgc gtagtcctgt
     7321 gtataggcga cgattcgatc ggcaagccgt gcggcgaccc tgttggcgag gtgcacggtg
     7381 aggccgacga cgcggttgaa cggcccgggt ggaagctgaa gatcgcagtg gtacgtcagg
     7441 gtcaccgggg tgccgccacg ccgagcacga acggtgagcc ccgcggcgtc gaacatgggc
     7501 aggtgcaggc tgagcacatc gtgctcgcgc atcaggcgag cagcccgcac gccgagcgtt
     7561 ggcatgatca cacccttgct cacgcgcagg gccaccggca cacgcacgat gtggaccccg
     7621 tccactacct cattgaccga caacgaagac gagtaccgcg aggtaagcac cgtcacttgg
     7681 tgcccgtggg cgacgagcgc gcgcgcgagg cgctcggcgt agatcgtcaa gccgctcacg
     7741 tgcggacgat agtaggtaac ccccaacagg acgcgcattg tcagccccta taccgccgaa
     7801 agccggtcac ggttggccgt gatccactcg gtcagtcggc ggatgccgtc ctgcggcgcc
     7861 acgctcggct cccaacccaa ctcgcggcgc gccttgccga tatcggcgat gaacaccgct
     7921 tggtcgcccg gccgactctc atcgaagcaa gggtcggcga gcctccgacc gagcacctcc
     7981 tcggccagag gcttgagctc ccaccagatc gagatggcgt tcttcggtcc accaccaacg
     8041 ttgtagatct gcccggccgc gacatcgatt cgctccaccg cagcgtcata gagcttgagc
     8101 agatcatcga tatagaggac atcgcggacc tgcttgccgt cgccgtagat ggtgatcggc
     8161 tggtccgtgg caagcgcgat aacaaaccag gcaagccagc cttgatcctc aactcccatc
     8221 tgacgtggtc cgtaaataca tgattgccgg aagacgacgg tcggcaggtc atagatgcga
     8281 gcgtaatctc ggacgtactg gtcgcccgca cccttggagc agccgtaggg cgagtggaaa
     8341 tcgaggggtt gtgactcatc caccccgttc ggcaggtcgc ggtaggcgtg gcgggtcgcc
     8401 agctcgactg tggcaacgcc ctccatggca ccataaacct tgttcgttga cgagtagagg
     8461 acgatcggcc ggcggcctgt cagccgaccc gcctcgagca cgttcagggt accaagagca
     8521 ttgacctcga aatcacggcg gggatcagcg accgagcggg tgacagccgt ctgcccggcg
     8581 agatgataaa tgacgtcgac atcctgaacg gctcgttcgc aggcatccgc atcggtcagg
     8641 ctggcaacga gcatatcgaa ggcatcaccg tggcgttccc gcagccagtc aatgttgtgg
     8701 tcgacgtggg gcctcgagag gtcgtcgaaa acggtgacgt ggtgtccggc tgccagcgcg
     8761 tggtcgacga cgttcgatcc gataaatcct gctccacccg tgaccagaac tgatcgtctc
     8821 tcctgcatcg cgtccatccc tctccgcgtg gcggcatcaa tcaagattgg caagtgggcc
     8881 cgggtcgatt acggtatttc gcagttcgca tcctagcatc gctcagcgag acggtccaag
     8941 cttgccagtg ccgaagtgcc tgaacgagag catgaggatg gcgccgaacg cccattcctg
     9001 ccgattccac agtagtatgg tcacatgcag attctcgtaa ccggcggcgc tggcttcatc
     9061 ggcagccatt tggtggatca gttgctcgct ggcggtcacc acgtcaccat cgcggacagc
     9121 ctgctcaccg gtcaccgggc aaatattgcc cacctgcgcg gcgagcggcg gctgcgcttc
     9181 gttcttcagg acatcgccga ccccctcgcc cctgaactcg ccgaaacgcc ttttgactgg
     9241 atctacaacc ttgcgtcgcc tgccagtccg cggggataca cgcggtatcc gctggaaacg
     9301 atccatgcca actcggcagg tgtctgg
//
LOCUS       DASYWL010000081         4878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_21993, whole genome shotgun sequence.
ACCESSION   DASYWL010000081 DASYWL010000000
VERSION     DASYWL010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21993"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1677)
                     /locus_tag="VGR22_03020"
     CDS             complement(94..1677)
                     /locus_tag="VGR22_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEV2127571.1"
                     /translation="MSTAVPSTNQALTADPRLDPERRDRLRGLLAGIVGALVLVAVVM
                     LLRPFTGGFSIIDVLADATLLAMPIGLFAWLLETFGTHAKTLFLVGLIALFVLIGGGL
                     GQLYAARTAGARRPMWARAQAYAIGIALGLGLVYLLVTDFVESAGPSLLRVTLALALG
                     AEAWAIVTALLLNALRHRGAPPEAMQHAGEGVDRRRAIATGATGVAMLGALGVIGWDI
                     TRVANRQTVGGPTAGELPPPITPIDDFYVISKNFIDPDPDRGDDWGVEIEGLVERPGR
                     ISRAELEELAGPDFVSTLTCISNEIAGPLIGTARWTGAPLGKVLERAGVQPTAVDVVG
                     LGEDGYTDSIPIEKALAPETHIVWGMNGEPLPRLHGTPVRLIVPGLYGIKNVKWLTKL
                     SVVGRDYQGYWQERGWTDEAIIKTQSHFDVPDAQDLLVAGTHEIGGVAFAGDRGISKV
                     EVSFDGGGTWREATIAENPGDAGLSWVIWKMDWQGKSGSYVLVVRATDGEGNLQTEEE
                     TSTLPAGASGYHRIRVGVA"
     gene            1874..2293
                     /locus_tag="VGR22_03025"
     CDS             1874..2293
                     /locus_tag="VGR22_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127572.1"
                     /translation="MAPAEIDLLWNGIRIRPAGWHRVWSLRRQIGVPYEQIVSVTHDP
                     ALARNGPLGIRMPGTNVPGYYTAGTYWRFWSRPRVRSFWVRRHAERTITLELRGHWYD
                     LICVEVDDPQAQVARIEAAIAAHASDRTDHPGMSAVR"
     gene            2328..3743
                     /locus_tag="VGR22_03030"
     CDS             2328..3743
                     /locus_tag="VGR22_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV2127573.1"
                     /translation="MTAPVEHLPDPHRPTWHFVLPANWMNDPNGLCQWKGTYHLFYQH
                     NPHGAFWGTMHWGHARSKDLVHWEHMPIALAPDPDGPDADGCFSGCMANVDGVATMLY
                     TGVRGDAQLTCIATSQDDDLITWEKYLGNPVIAALPAELDTTIFRDHSVWRENGIWYQ
                     IIGSGIRGVGGTALLYRSSNFYRWEFVDSLVELDRVSQGIGKGATGWECPDFFALDGR
                     HVLVASMWDQEPIVVAYFVGDYADHRFTPTHEGVLDPGTAYYAPQTLTDEAGRQIQFG
                     WVREQRSTEAQIEAGWSGVMSLPRVLSLLPDGSLAATPASEVETLRGRHVRLPGGQIV
                     AGEPLPLDGISGNSLELLVTLDPSFRETVAIEVLRSEDGGETTIISFDATSGTLTLNT
                     TGASHSGQVEGGVYALRHVGHSDDAVTLRIFVDHSVVEVFLNDARPITARAYPESLDA
                     TGVTVSTPEGVASIDIWEMRP"
     gene            3901..4083
                     /locus_tag="VGR22_03035"
     CDS             3901..4083
                     /locus_tag="VGR22_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127574.1"
                     /translation="MGKDMRKEDGVGDWKRDRVGSAESGTNPMVITRMESGYAVMGDT
                     QFLPGYCVLLASPRVT"
     gene            4128..>4878
                     /locus_tag="VGR22_03040"
     CDS             4128..>4878
                     /locus_tag="VGR22_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127575.1"
                     /translation="MALLGEAIERVCADEGLCRVNYEILVNTDTFLHAHVFPRYTWEP
                     ADRITMPVFRYPDENWRLPAYQYDDARHGALRLRIRETLELLTRAERGHDHHCEDATI
                     GRIVMEPFSFLSGNLRGWSSPTQDGEHAWVYRAPHYAALLRDHAPHLIGFQEFHPSNG
                     DDVAPALPFHAWELGCECDTEQYTPIYWDTRRFGLIRTDSCWLNRWRTVKAVDWGANH
                     ERAVTWVELMDRQADRIVLVVNTHLDHISEPA"
BASE COUNT          834 a         1645 c         1596 g          803 t
ORIGIN      
        1 gcccggggcg gggatccttc gactccggcc cgtcgggcct ccgctcagga tgacatcacg
       61 ttcacagatc tctccgtggc ttccatccat accctacgct accccaactc ggatgcggtg
      121 gtagccactg gcaccggcgg gaagcgttga ggtttcctcc tcggtctgaa ggttgccctc
      181 gccgtcagtg gcgcggacca ccagcacgta gctgcccgac ttcccctgcc agtccatctt
      241 ccagatcacc cagctcaggc ccgcgtcacc cgggttctcg gcgatggttg cctcccgcca
      301 cgtcccgcca ccatcgaagc tcacctcgac cttgctgatc ccgcgatccc cggcgaaggc
      361 gaccccgccg atctcgtgtg tgcccgccac cagcaggtct tgcgcatccg gcacgtcgaa
      421 gtggctctgc gtcttgatga tcgcctcgtc ggtccagccg cgctcctgcc agtagccctg
      481 gtagtcacgg ccgaccaccg acagcttcgt cagccacttc acgttcttga tgccgtagag
      541 cccgggcacg atgagccgca ccggcgtccc atgcaggcgc ggtagaggtt cgccgttcat
      601 tccccacacg atgtgcgtct ccggcgcgag cgccttctcg atcgggatcg aatcggtgta
      661 gccgtcctcg cccaaaccca cgacgtcgac cgccgtcggc tgcacaccgg cgcgctccag
      721 caccttgccg agcggcgcgc cggtccaccg ggcggtgccg atcagcgggc cggcgatctc
      781 attgctgatg caggtcagcg tcgagacgaa atccggccct gcaagctcct cgagctcggc
      841 gcgcgagatg cgtcccggac gctccacaag cccctcgatc tcgacgcccc agtcgtcgcc
      901 gcggtccgga tcggggtcaa tgaaattctt cgagatgacg tagaagtcgt cgatcggcgt
      961 gatcggcggg ggcagctccc cggcagtggg gccaccgacc gtctgccggt tcgccaccct
     1021 cgtgatatcc catccgatga cccccagcgc gccaagcatg gccacgcccg tcgcaccggt
     1081 cgcgatcgcc cgccgccgat cgaccccctc tccggcatgc tgcatcgcct cgggcggtgc
     1141 gccgcggtgg cgcagcgcgt tcagcagcag cgcggtgacg atcgcccatg cctcggcacc
     1201 gagcgcgagt gcgagggtca cccgcagcag gctggggccg gcggactcga cgaagtcggt
     1261 gacgagcagg tagacgagcc cgagcccgag cgcgatgccg atggcatacg cctgcgcgcg
     1321 tgcccacatc gggcggcgcg ccccggcggt gcgtgcggca tagagttggc ccaggccgcc
     1381 gccgatcagg acgaacagcg cgatcagccc gaccagaaag agggtcttgg cgtgcgtgcc
     1441 gaaggtctcg agcagccagg cgaagaggcc gatcggcatc gccagcaggg tcgcgtcggc
     1501 gaggacatcg atgatcgaga acccgccggt gaaggggcgc agcagcatca ccacggccac
     1561 gaggaccagg gccccgacga tgccggccag caggccgcgc aggcgatcac gacgctcggg
     1621 atcgagtcgt gggtccgctg tcaaggcctg gtttgtggat ggtacagcag tcgacatgct
     1681 cggagccctc ccgatgcccg gcggcggcga aggtaaagtg cgctcgtgta tgctttcgga
     1741 cgtactggat gtggctggtg acgcagtgcg ccacgtcgcc ggatcggcgc cgcggtcgta
     1801 tcgctcaatc atgccccact gccgtggcga ccgccgtgca cgacctacac cgagacagca
     1861 agaggagcgc accgtggcgc ctgccgagat cgatttgctc tggaacggaa tccggatccg
     1921 tccggccggc tggcaccgcg tgtggtcgct gcggcggcag ataggtgttc cgtacgagca
     1981 gatcgtatcg gtgacccacg accccgcgct ggctcggaac gggccactcg gcatccggat
     2041 gccggggacg aacgtgccgg gctactacac ggcgggcacg tactggaggt tctggagccg
     2101 accgcgggtg cggtcgttct gggtgcgccg ccacgccgag cggacgatca cactggagct
     2161 gcgagggcac tggtacgacc tcatctgcgt cgaggtggat gacccgcagg cgcaggtcgc
     2221 gcgcatcgag gccgccatcg ccgcgcatgc ttctgatcgc accgaccatc ccgggatgag
     2281 cgccgttcgc tgagggcacc cgtcggcatg cgtgcaggag aatggcaatg accgcaccgg
     2341 ttgagcacct gccggatcca caccggccga catggcactt cgttctgcct gcgaactgga
     2401 tgaacgaccc gaatgggttg tgccagtgga aggggaccta ccacctcttc tatcagcaca
     2461 atccacacgg ggcgttctgg ggcaccatgc actgggggca tgcgaggagc aaggacctcg
     2521 tgcattggga acacatgccg atcgcactcg ctcccgaccc ggacgggccg gacgccgacg
     2581 gctgcttctc cggctgcatg gcgaacgtcg atggcgttgc cacgatgctc tacaccggcg
     2641 ttcgcggaga cgcccagctg acatgcatcg ccaccagtca ggacgatgac ctcatcacgt
     2701 gggagaagta cctgggcaac ccagtcatcg ccgcgctccc agccgagctc gacacgacca
     2761 tcttccgaga ccactcggtc tggagagaga acggcatctg gtaccagatc atcggctcgg
     2821 ggatccgggg ggttggcggc acggcgctcc tctaccggtc gagcaacttc taccgttggg
     2881 agtttgtgga ctcgctcgtc gagctcgatc gcgtctccca agggatcggg aagggcgcaa
     2941 cgggctggga gtgtcccgac ttcttcgctc tagacggacg gcacgtcctc gtcgcgtcga
     3001 tgtgggatca ggagccaatc gtagttgcct acttcgtcgg cgactatgcc gaccaccgct
     3061 tcacaccgac gcatgaaggc gtcctcgacc cgggcacggc gtactacgct ccccagaccc
     3121 ttacggatga ggccgggcgc cagatccagt tcggctgggt gagggagcag cggagcaccg
     3181 aggcccagat cgaagcgggc tggtccggcg tgatgtcgct gccccgcgtg ttgtcgttac
     3241 tccccgacgg gtcgctggct gctacccctg cgtccgaggt cgagacactg cgagggcgac
     3301 acgttcgact gcccggcggc cagatcgtcg cgggcgagcc cctgcccctt gacgggattt
     3361 ccgggaactc gctagagctg cttgtgacgc tcgacccgtc gtttagggag acggtggcga
     3421 tcgaggtcct tcgctccgag gatgggggcg agacaacgat catatcgttt gacgccacat
     3481 ccggcacgct cacgttgaac accacaggcg ccagccattc cggccaggtg gagggcggcg
     3541 tctatgccct gcgccatgtc ggccattcgg acgatgccgt gacgctccgc atcttcgtcg
     3601 accactcggt cgtcgaggtc ttcctcaatg acgcgcgtcc catcaccgcc cgcgcctacc
     3661 cggagagcct cgatgccacc ggcgtcaccg tcagcacacc ggagggcgtg gcatcgatcg
     3721 acatttggga gatgcgcccg tagggtcggg cgaacgacag cggccgcggg cccgggatcg
     3781 acaccgggac cgtgcacacc gccgctccgg ctgagaccac ccacggtggc caatcggggt
     3841 gagcgacgcg atgaccatcc gctgcgaggt tgacgacacg cgtaggaaag gatgctacgg
     3901 atgggcaagg acatgcggaa ggaggacggg gtgggagact ggaaacgcga ccgggttgga
     3961 tcagccgaat ccggcacgaa ccccatggtg atcacgcgga tggagagcgg ctatgccgtg
     4021 atgggtgaca cacagttcct gccagggtac tgcgtcctct tggcatcccc gcgcgttact
     4081 tagctgagga cctgccgctc gccagtcgcg tctcctttct gcgcgacatg gccctcctcg
     4141 gggaagccat cgaacgggtc tgcgccgacg agggattgtg ccgcgtcaac tacgagattc
     4201 tggtcaacac ggataccttc ctccacgccc atgtcttccc gcgttatacc tgggagccgg
     4261 cggaccggat cacgatgccg gtcttccgct accccgacga gaactggcgc ttgccggcgt
     4321 atcagtacga cgatgcacgc cacggcgcgc tccgattgcg catcagagag acattggagc
     4381 tcctgacgcg ggcagagagg ggtcatgatc accattgcga ggatgccacg atagggagga
     4441 tagtcatgga gccattctcg tttctctccg gcaacctgag ggggtggtcg tcgccaacgc
     4501 aggatggtga gcacgcgtgg gtgtaccgtg ctccccacta cgccgctctg ctcagggacc
     4561 atgcgccgca cctgatcggc ttccaagagt tccaccccag caatggcgac gatgttgcgc
     4621 ccgccctgcc cttccacgct tgggagttgg ggtgcgagtg cgataccgag cagtacacgc
     4681 ccatctactg ggatacgcgg cggttcggcc tgatccgcac cgatagctgc tggctcaaca
     4741 ggtggcgcac cgtcaaggcg gtggactggg gcgcgaacca tgagcgcgcg gtgacctggg
     4801 tcgaactcat ggaccgccag gcagaccgaa tcgtgctcgt ggtcaacact cacctcgacc
     4861 acatcagtga gccggcac
//
LOCUS       DASYWL010000082         3515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_78655, whole genome shotgun sequence.
ACCESSION   DASYWL010000082 DASYWL010000000
VERSION     DASYWL010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78655"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VGR22_03045"
     CDS             <1..287
                     /locus_tag="VGR22_03045"
                     /inference="COORDINATES: protein motif:HMM:NF018531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HEV2127576.1"
                     /translation="TSLVRSIRKVLRASIANQGTTFATFENPYGERGNNAGALKVYGR
                     AKRGLPCERCGRPLGWFKLGGRGTSYCPHCQSARVPVGGESRMPAVRMKL"
     gene            complement(301..510)
                     /locus_tag="VGR22_03050"
     CDS             complement(301..510)
                     /locus_tag="VGR22_03050"
                     /inference="COORDINATES: protein motif:HMM:NF022829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HEV2127577.1"
                     /translation="MAKLNPIELQQALKGVDYPADKIALAEAAERNGASGDVRNAIDR
                     LPDKQYEKPTDVTEAVDFGADSDEN"
     gene            complement(710..1657)
                     /locus_tag="VGR22_03055"
     CDS             complement(710..1657)
                     /locus_tag="VGR22_03055"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEV2127578.1"
                     /translation="MTTHRFEPSRYQNTFSSHEPVLRIHAGDTVVTTTVDARGVDHGD
                     QEVAARPNPQTGPFFIEGAEPGDTLVVRFDRIEANRATGWTSTALASNVVDPDFVPSL
                     PTTPRVHWTIDRAAGTVAPVEPEEGLERLALPLDPMLGSFGVAPKDGQAITSFTSGEH
                     GGNMDYRGFRQGTVAYLPVFVPGALFQLGDGHAVQGDGEISGTGVETSMKVEFTVDVR
                     KGWKIGWPRGETAAHIFAAGNARPLDQALQHATTEMTRWLEQDYGLSVRASSLLMGQA
                     VEYEIGNVYDPAYTVICKMPKAVLGMLASNARLRTAASG"
     gene            1922..3067
                     /locus_tag="VGR22_03060"
     CDS             1922..3067
                     /locus_tag="VGR22_03060"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEV2127579.1"
                     /translation="MTASWRLLTIRGIDIGLHWSMVIVFGLLTVSLATAYFPETSPDL
                     SEGAAWIMAVVAAVFFFASILFHELGHSFVALRYELPVDRITLFIFGGIAQISERAKS
                     ADVELKVAAAGPAVSFALSAIFGLAWFVTQDIDVIAAPALWLATLSLILALFNLLPGF
                     PLDGGRILRALVWSTTGNERKAARVAMVSGQFVAFGLIGLGALLMFTGNFTNGLWLIF
                     IGWFLQNAAVSEATGTSVEIALRGAEVRQAMGPAESQVPGRLMLRQLIDEYVLPAGER
                     HFIVVDGDTPRGIVTLRDITQVPQDRWDWTPIREIMKPWSQLTCVTPDSKLLDAMKIM
                     DEKQVRQLPVIENDEALGLLTREEVLRYVRLRMELEDGEPDERVVAQ"
     gene            3096..3500
                     /locus_tag="VGR22_03065"
     CDS             3096..3500
                     /locus_tag="VGR22_03065"
                     /inference="COORDINATES: protein motif:HMM:NF012601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127580.1"
                     /translation="MDAAELSPSDRELIETAKRTADRLHLDNAHEVAAAVRTRSGEVF
                     SGINHKARMRFADVCGEVAALSVMVGAGRRDPEAIVAMWGDGNGTYDLMPPCGRCREL
                     ISDFDPETWVIVGTLQPPYKVKVCELLPLKAW"
BASE COUNT          593 a         1148 c         1105 g          669 t
ORIGIN      
        1 tcacgagcct cgtgcgctcg atccggaaag tcctgcgagc ctcgatcgcc aaccagggga
       61 ccacgttcgc cacctttgag aatccatacg gcgagcgagg caacaatgcc ggggcgctca
      121 aggtctatgg tcgtgcgaag cgcggtcttc cgtgcgagcg gtgcgggcga ccacttggct
      181 ggttcaagct cggcggtcgg ggaacgagct actgcccgca ctgccagtcg gctcgcgtgc
      241 cggtcggcgg cgaatcgcgc atgccagcag tccgcatgaa actgtgagtg acccagcttc
      301 ctagttctcg tcggaatctg cgccgaagtc cacggcctcg gtcacgtcgg tcggcttctc
      361 gtactgcttg tccggcagcc gatcgatagc attgcgaaca tcgccgctgg cgccgttcct
      421 ttcggccgcc tcggctaggg cgatcttgtc ggccgggtag tcaacgccct tcagcgcctg
      481 ctgcaattcg atcggattca acttcgccat ctgcactgtt cctccgcgcg tgtcgcttgg
      541 accatccgcc gagccgcatg agactcgcgt acggctcttt tcgcagtgcg cgtgccatga
      601 tccaggcgct cccctgcaag gctcaagctc atcggcaccc gatgctcaag ccggcccagg
      661 catcgcccac tcccatcgct ccccctcatc cccatgacaa aacggcggct tacccgctcg
      721 ctgccgtacg gagccgggcg ttgctcgcga gcatcccgag gactgccttc ggcatcttgc
      781 agatgacggt ataggccgga tcgtaaacgt tgccgatttc atactcgact gcctgcccca
      841 tcagcaggct cgatgcgcgc acgctgaggc cgtagtcctg ctccagccag cgtgtcatct
      901 cggtcgtcgc gtgctgaagc gcctggtcga gggggcgcgc gttgcccgct gcgaagatat
      961 gggcggcagt ctcaccgcgc ggccagccga tcttccaccc cttgcggacg tcgacagtga
     1021 attcgacctt catggacgtt tccacgccag tgccggagat ttcgccgtct ccctgcacgg
     1081 cgtgaccatc gcccagctgg aaaagcgcgc cagggacgaa gacgggcagg taggcgaccg
     1141 ttccctgccg gaagccgcgg tagtccatgt tcccgccatg ctcgccggac gtgaacgacg
     1201 tgatcgcctg cccgtccttt ggagcgacac cgaagctccc gagcatcgga tcgagcggca
     1261 gcgcgaggcg ctccagcccc tcctctggtt ccacgggcgc gacggtacca gcggccctat
     1321 cgatcgtcca gtgtacgcgg ggcgtcgtcg gcagcgacgg cacgaagtcg ggatccacaa
     1381 cgttgctcgc cagtgccgta cttgtccatc cggtcgcacg gttggcctcg atccggtcga
     1441 accgcacgac gagcgtatca ccaggctcgg ccccctcgat gaagaacggt ccggtctgcg
     1501 ggtttggccg tgcagccacc tcctggtcgc cgtgatcgac ccctcgcgca tcgacggtgg
     1561 tcgtgaccac cgtatcgccc gcgtggatgc gcagcacggg ctcgtgcgag ctgaaggtgt
     1621 tctggtaccg gctgggttcg aatcgatgtg tcgtcacaaa caccatcctc tcgcctgtga
     1681 tatctctact gtcgtggcgt gtgcgttcac caacagtgca gcccaagaag acagagaatc
     1741 tgggcgaaac cgtctgtggg gacgtgcgcc tcgacacccg tgacgcgatt attgcacccg
     1801 atcgttgaat gcgcgccctg cgacgacgtc tagcgccccc gacactcgct cggcgttgtc
     1861 tcgatcttgc gatactgcag accggtacag gttgtgctgc atacatacaa ggagaatgtg
     1921 aatgaccgcc agctggcgcc tcctgacaat tcggggtatt gacattggac tgcactggag
     1981 catggtgatc gtcttcgggc tgctcaccgt ctcgcttgca acggcgtact tcccggagac
     2041 gtctcccgac ctctccgagg gcgccgcctg gatcatggcc gttgtcgcgg cggtgttctt
     2101 cttcgcctcg atcctgttcc acgagctcgg ccattccttc gtggcgctca ggtatgagct
     2161 gccggtcgac cgcatcaccc tgttcatctt cggcggtatc gcccagatct ccgaaagggc
     2221 gaagtcagcg gacgtcgaac tgaaggtggc ggccgccggg cctgctgtta gcttcgcctt
     2281 gagcgcgatc ttcggtttgg cctggttcgt cacgcaggat atcgacgtga tcgccgcacc
     2341 ggcgctctgg ctcgccaccc tcagcctgat tctcgcgctc ttcaacctgc tgcccggctt
     2401 cccactcgac ggcgggcgta tccttcgggc gctcgtctgg agtacgaccg gcaacgagcg
     2461 caaggcggcg cgggttgcaa tggtcagcgg gcaattcgtc gccttcggac tcattggcct
     2521 cggcgcactg ctcatgttca ccggcaactt caccaacggc ctctggctga tcttcatcgg
     2581 ctggttcctg cagaacgcag cggtatcgga agcgacgggc acatcggtcg agatcgcgct
     2641 ccgtggcgct gaggtgcggc aggccatggg tccggccgaa agccaggtac cgggccggct
     2701 gatgctgcgc cagttgatcg atgagtacgt gctgccagcc ggcgagcgcc acttcattgt
     2761 ggtcgacggt gacacaccac gcggcatcgt gaccctccga gatatcaccc aagtgccaca
     2821 ggaccgatgg gactggacgc cgatacgtga gatcatgaaa ccctggagcc agctcacttg
     2881 cgttacaccg gactcgaagt tgctcgacgc gatgaaaatc atggacgaga agcaggttcg
     2941 ccagttgccg gtcatcgaga acgacgaggc gctcgggctg ctcacgcggg aggaggtcct
     3001 ccgttacgtc cggctccgca tggagctcga ggacggcgag ccagacgagc gcgtggtagc
     3061 ccagtagctg cattgccacg tcgaggagag attggatgga cgcagccgag ttgtcgccgt
     3121 cggatcgtga gctcatcgaa acagccaagc ggacggcgga caggctgcac ctggataacg
     3181 cgcacgaagt cgcggccgcc gtccgcaccc gttccggcga ggtcttctcg ggcatcaacc
     3241 acaaggctcg catgcggttc gccgatgtct gcggtgaggt cgccgccctg agtgtcatgg
     3301 taggcgccgg tcgccgcgac ccggaagcta tcgtggcgat gtggggagat ggaaacggca
     3361 cctacgacct catgccgccc tgtgggcgat gccgggagct gatcagcgac ttcgatcccg
     3421 agacgtgggt catcgtcggg acgctgcagc cgccgtacaa ggtgaaggtg tgcgagctgc
     3481 tgcccctcaa ggcgtggtag aaagcgagca cacgg
//
LOCUS       DASYWL010000083        18213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_153642, whole genome shotgun sequence.
ACCESSION   DASYWL010000083 DASYWL010000000
VERSION     DASYWL010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 18213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18213
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153642"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..493)
                     /locus_tag="VGR22_03070"
     CDS             complement(71..493)
                     /locus_tag="VGR22_03070"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HEV2127581.1"
                     /translation="MGDVDINWLAVILAGIAGAGVSWLWFSKYLFRNRWMHLAGLTLE
                     RVERGTSVAAAITGVVSLLTAYVVAYVASLIEAHSGDGDLASALGAAFWLWFGIAAAT
                     VIVHDAFDQQPVTKMAISAGERLVTLMVMGFIIGLFGV"
     gene            complement(884..1411)
                     /locus_tag="VGR22_03075"
     CDS             complement(884..1411)
                     /locus_tag="VGR22_03075"
                     /inference="COORDINATES: protein motif:HMM:NF001095.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEV2127582.1"
                     /translation="MYAFGMMSEQANQHPIYLDADACPVKDVVYKVAARYSVPLKVVA
                     NGYLRIPDIAGLDAEMVTVPGSPDAADDWIAERAVQGDLVLTADIPLAAKVVDNGVRC
                     LDFRGTEFTSSRVVDALASRDLNAYLRSIGETTGGPKAFSPKDRSKFASTLDAAVGKM
                     ARDTKHKTYGPRDNS"
     gene            1494..2363
                     /locus_tag="VGR22_03080"
     CDS             1494..2363
                     /locus_tag="VGR22_03080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEV2127583.1"
                     /translation="MHIGAIFPQSEIGADPIAVRDIAQAAEDLGYHHLLAFEHVILPG
                     PPEYRGYAGAATAEQPFHEPLVLFGYLAALTQRIELVTDVLVLPQRQTVLVAKQAAEV
                     DVLSGGRLRLGIGVGWIPNEFAALGADYSNRGTRSEEQIVVLRALWTEPVVTFRGRWH
                     QIEAAGISPLPVQRPIPIWIGGRVEATLRRVARMGDGWFPLDEHPDDQAREKVERLRM
                     YTVEAGRDPTAVGIEGFLSVSAVPRERWAKHAEGWRTIGATHLAVSTIGAGFASPQQH
                     IDALQQVKEVLRP"
     gene            complement(2522..3388)
                     /locus_tag="VGR22_03085"
     CDS             complement(2522..3388)
                     /locus_tag="VGR22_03085"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV2127584.1"
                     /translation="MPSPEEALQAIRDMRSPLLQFYVGPSAICPTDDPVDRFSRQQVT
                     GVAALVEKHNAGLHTHAYAGQIAAAHAIASEVLGPHACLAHCAGISMEEIEILARTGA
                     SGSFGPLTHAYVKARFPLIEALEAGVNAVFSTDGSAPDRSFDLIDQARVGMQLQRVHF
                     NDDRLLPAGKAPGMITIDAARALGMQDKIGSIEVGKQADVIVVDWHQTHLAPRILAPL
                     RLVGHASGQDVRTVIVDGQVRMRDRTVPGVDEAAIMDAATDALMATWERSGLGDIHFQ
                     HPDIWRGIRYSG"
     gene            complement(3375..3944)
                     /locus_tag="VGR22_03090"
     CDS             complement(3375..3944)
                     /locus_tag="VGR22_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127585.1"
                     /translation="MLDLAIHNGTVITMDPDRRVLDTVAIGIRDGRIVQIESDSSALE
                     QANEVIDAKGDIVMPGIVDTHGHAGHSLTRGLGEGLDEGGWMKIVEHLYFQASDVAFW
                     EAESRLAALQRLRFGVTTSVSMTGSSPRVDDARYAVAASTGYREFGLRHIVAAGPPNG
                     PWPRRYTERHPDGSSREIEVDLEHAIAVT"
     gene            complement(3973..5847)
                     /gene="iolD"
                     /locus_tag="VGR22_03095"
     CDS             complement(3973..5847)
                     /gene="iolD"
                     /locus_tag="VGR22_03095"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR04377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HEV2127586.1"
                     /translation="MTTIRATMAQALVRYLAAQKVERDGEARPFFAGMFGIFGHGNVT
                     GIGQALEEHRDLLRYYRPQNEQAMVHTAAAYAKATNRLGTFACTSSVGPGATNMVTGA
                     ALATVNRLPVLLLPGDTFANRAPHPVLQQLEYTLAGDVSANDTFRPVSKFFDRIVRPD
                     QLLMSLPEAMRVLIDPAETGAVTLAMPEDTQTEAFDYPASFFEERVWKIRRPVPPQDA
                     LEEAARLISNSENPLIIAGGGVIYSGASDALDDFATEFGIPVSESQAGKGAIPWDHVW
                     NIGPIGANGGFAANRIASDADVIIAIGTRLGDFVTASKTAFQNPNARFIGINVAGMDA
                     HKMGAVPLTGDAWATLEALGEVLRAEGTVRDIYATGKPVQDLKREWEAEVDRLCAIEG
                     DGEKLSQAQVLGLVNEAAGDHGTVINAAGSMPGDLLKLWRARDPKGYHVEYGYSCMGY
                     EIPAGLGVKLADPGREVFVMIGDGSYLMLHTEIVTAVQEGVKFVVVVVDNHGFQSIHG
                     LQMGSGTPSFGNELRYRSEGGVLDGPPVPVDFATNAESLGARAIRATTADELREALVE
                     AKTESRVTVVTVEVDPEPRVPNYEGWWDVPIAEVSDEPSVNDARAEYDEAIKQQRVVW
                     "
     gene            complement(6037..6948)
                     /gene="iolB"
                     /locus_tag="VGR22_03100"
     CDS             complement(6037..6948)
                     /gene="iolB"
                     /locus_tag="VGR22_03100"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF016826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HEV2127587.1"
                     /translation="MTPANELLMKPRDPGDHGLVMQVGPEEAGWEYIGLEVRRIGEGE
                     TFTFSSPNDELCLVPTVGEAAVGLGDEEWEISRPGTVFGGKPTALYLPVGSDVRIETD
                     TGIEVAICRSRATETFPPKLIRPQDIAIEIRGSGNAARQISHIITPDFPAHRLLVVEV
                     FTPSGNWSSYPPHKHDASRMPEEADLEEIYYYRIHPQDGFALQRLYGPGYDHAWTIRD
                     GDLLQVPEGYHAFCVAHGYTGYYLNVLAGNEATRTMQPADDPRYAWVRDTWTEKQVSG
                     LETWEDIEMRINGMAGRRDSLTGGDRT"
     gene            complement(7164..8366)
                     /locus_tag="VGR22_03105"
     CDS             complement(7164..8366)
                     /locus_tag="VGR22_03105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV2127588.1"
                     /translation="MVTSTLPETSLPPVPGPQFPQNGRQLSIIEIAESLAEIAGRNAP
                     AHDRDNTFPHDTFQALIDAGYHTLTIPEEMGGMGATPLEVMMAQERLARGDGSVALAI
                     CMHLGHIYGVATSDEWPQDLRKQVIDQVVNEGGLYNTAASEPGLGSPSRGGFFATTAE
                     RTDDGCWKLNGRKTWTTLAPALRWAGVGATITENGESVEKTSFLVPMDAEGVEIDETW
                     DNMSMSATGSHDLVLTDVVVPDSHRLPVSGKPSNSPWSILTTAVYLGVSVAARDFTIA
                     FAKTRKPAAMGGETIATLPNVQQRVADIELKLLAARSTLYGAVQQWEDHPEQRDELGW
                     QLAGAKVLVTNNVIDITDQALRIVGAAGLQRKHPLERYFRDARAGLDNPPLDDVATGI
                     IGKAVLDQ"
     gene            8592..9278
                     /locus_tag="VGR22_03110"
     CDS             8592..9278
                     /locus_tag="VGR22_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127589.1"
                     /translation="MRLFRVMAPVLLAFSMVAGMTTATAAQDVPEATPETALPEGLEK
                     WYARTFSADLFAMFGPEASPAADPEGWFLLSTNVLKFETEEQAAAAAEELLAQFEEEL
                     AAEDLQLEDVELDLELDYTAQRAEEEDQGLTTVVLEAVAQDGQYVYAVVGVTLGVDPA
                     PLIERAIQAMQEGDVSGEEDFDIVGMSEGGLWAKLPTVEEITAEAPALVTVEDTIFFP
                     APELQATPAA"
     gene            complement(9531..10280)
                     /locus_tag="VGR22_03115"
     CDS             complement(9531..10280)
                     /locus_tag="VGR22_03115"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV2127590.1"
                     /translation="MTRIAIFGDIHGNIQAARTVLEDIDSAGVDRLYCLGDLIGYGAS
                     PNEVIDLVRERDIPTIMGNYDDGVGSGRDDCGGAYRDAGERERGQASLMWTRAQVDAE
                     RKAWLRSLLPEIRVEAHGHRIRLVHGSSRRMNEYLFEDRDERSLERIAAVADCDVLVF
                     GHTHIPWDRIINDVRFINAGSVGKPKDGDPRAGWLLLEVTPESVVATMQRVNYGIATA
                     AAAIRAADGLPDHFAADTKTGGMPTSDARSG"
     gene            complement(10277..11377)
                     /gene="arsB"
                     /locus_tag="VGR22_03120"
     CDS             complement(10277..11377)
                     /gene="arsB"
                     /locus_tag="VGR22_03120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HEV2127591.1"
                     /translation="MSATPSVTTTVDRQRDEQLQKLGVIDRFLPLWIFAAMAIGLLLG
                     RIFPDLGEQLDRVKIAGVSVPIAIGLFWMMYPVVAKVRYGSLGSVASNGRLLSLSVAL
                     NWIIGPVLMFALAWAFLPDEPHYRTGLILIGLARCIAMVLIWNQLACGDGEVAAVLVA
                     INSLFQILMYSVLGWLFLSVIPGWFGADSAALDVSMADIALNVGIFLGIPLFAGALTR
                     FSLVRKKGEHWYEHTFLPKIGPTALLGLLYTIVILFSMQGDRLVELPMDVLRISLPLL
                     AYFGIMFGIAFVWAWRLGFSYEETAAIAFTAAGNNFELAIAVAIGDFGISSGEAIAGV
                     VGPLIEVPALIALVYVALWLRRRLFTPKGAPA"
     gene            11589..12386
                     /locus_tag="VGR22_03125"
     CDS             11589..12386
                     /locus_tag="VGR22_03125"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV2127592.1"
                     /translation="MGETISAVLLDLDETILYDELATEAAFSATATHAAEIAAVDPGC
                     LIVAVQEEARALWLEGPDPAWCDDIGTSEVEGLRARFEGNDPRTVAMRAWGPGFRSES
                     WKRALRRCGIDDDMLASELDARFEQERVSTNPFIPGVEETLDQLEERYRLAIVTNGLP
                     DVQREKLVRTGLMDRFDVVVISGELGFGKPDRRMYDETVRQLRLIAGECVMVGDNFRR
                     DVAGAQEAGIRGVWISCGKDLPDTDVTPFLVVESLAELPSKLASCRG"
     gene            complement(12574..14127)
                     /locus_tag="VGR22_03130"
     CDS             complement(12574..14127)
                     /locus_tag="VGR22_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEV2127593.1"
                     /translation="MSDGKSNGKTNRKPQADKPALTTRQGHPVHNNQDLRTVGNRGPA
                     TMENYQFLEKISHFDRERIPERVVHARGTIAHGVFEAYGTIGDEPASTYTRAKLFQEK
                     GKKTPVTVRFSTVAGGRDSSEVARDPRGFAVKLRTEDGNWDLVGNNLPVFFIRDAIKF
                     PDFIHSQKPDPVTNEANVPQRAFDFWAASPESMHMLTWLFSPRGIPASYRHQDGFGVN
                     TYRMVNDKGEAVLVKYHWKSQQGIKSLTAAEAAEIQAKTLGSHTKDLYDAIEAGDYPQ
                     WELNVQIMSDDEHPELDFDPLDDTKTWPEDQFPLLPVGMMTLNENPKNQFDENEQIAF
                     GTGVLVDGLDFSDDKMLVGRTFSYSDTQRYRVGPNYLQLPVNRPVVETRTNQEGGQMS
                     YGVDKRGADQSVNYEPSVLAGLSEADDSYTLYQPYVDGNVVKEPIDRTNNYKQAGERY
                     RTIEQWERDDLVLNLVTGLSQCDQVLQDMMVEHFTKCDEEFGQRIADGLKIKMSEMSK
                     MGTEQQQRV"
     gene            14375..15304
                     /locus_tag="VGR22_03135"
     CDS             14375..15304
                     /locus_tag="VGR22_03135"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEV2127594.1"
                     /translation="MSERIQVILDTDIGTDVDDCLALAVLLGSPEIDLVGVTTVYGDV
                     ALRARMVRKLLHLRGRDEVPVHEGVREPLVRNRQVYWPGHEGVGLLEAGDDERFGQGP
                     GQHAVDFLIERVMRQPGEVHLLAVGPLANVAAAMIREPGLASNLVHMTIMGGYIAPWT
                     GGRGTAEHNILSDPEAARVVFASGAPISLVPLDVTLKTVITREDVAEIREVGTLFHDA
                     VADQVMLYPPFENRGGQTFLHDPLAAAGIVRPDLLEWHELAVDIELAGRLTTGMTVAR
                     QPGPQAPATARVALGVDVIEAQRFILDRVCARQ"
     gene            complement(15445..15996)
                     /locus_tag="VGR22_03140"
     CDS             complement(15445..15996)
                     /locus_tag="VGR22_03140"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEV2127595.1"
                     /translation="MAEITNTADALEAIRKVRQVRQYEPGSVSDEDLNRILEIARWSG
                     SSRNTQPWRFVIVEDKDVLARLAELRPNITWVANGALAIALVFPGENDIHEAYDEGRV
                     SERVMIAAEMLGLGAGTAWFAENEGVAKQILGVPDDMVLHSVVVLGPYRTSKDPRPTG
                     PKPGRKPLDELVSYGKWGQKQRS"
     gene            complement(16145..16552)
                     /locus_tag="VGR22_03145"
     CDS             complement(16145..16552)
                     /locus_tag="VGR22_03145"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV2127596.1"
                     /translation="MDRHESLGDDVTWWTLPFLLVTIAASAVMIGALNYVINMIGFWE
                     PSAQSALPTLVALMIDFAKLPLDVFNLVIRGLVTVVIPYAFISYFPALLLLDRDAPWR
                     WLGLATPLVATLVVAITAWLWGEALNRYQGVGH"
     gene            complement(16489..16968)
                     /locus_tag="VGR22_03150"
     CDS             complement(16489..16968)
                     /locus_tag="VGR22_03150"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV2127597.1"
                     /translation="MNIAIPSIAQVRRNARLYLTLQLAQLRAAVEYSGDFWIGIIGAM
                     LQQAAGLVFIAALFTQIPEVGGWTAWNVAILYGLAMIPKGLIEFLCDGPRMLRTQVNT
                     GEFDRVLVRPISPALQSATAIASIHGFGQLGLGIIVLWIGMSRSEMTLPGGRYPSCW"
     gene            complement(16972..17160)
                     /locus_tag="VGR22_03155"
     CDS             complement(16972..17160)
                     /locus_tag="VGR22_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127598.1"
                     /translation="MAPHVLPMRGIVYTPVTLYLGKAEGWTVVQMLGMQLVWLAALWV
                     FADWAWRAVFDAVEVQGG"
     gene            17405..>18213
                     /locus_tag="VGR22_03160"
     CDS             17405..>18213
                     /locus_tag="VGR22_03160"
                     /inference="COORDINATES: protein motif:HMM:NF023650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan binding domain-containing protein"
                     /protein_id="HEV2127599.1"
                     /translation="MVQQTFPDVAEASDGAPIWESMFGRITDFLRGVVLPDEQHAPIR
                     ITSTGDSVLDGDDPSPMGRLGRLIPRLLLVLAVALLVAAIALFGFHAAYADKVYPAIA
                     VGDVSVGGMTVEQAVATVEQRAAEFERGTVTFTYNGQTWTPTLAELGASIDVESSVDA
                     AWELGRDESAVDRLGFTRQLLRSDQRVPLRTTVDLTVLAGWFEQVNADIDQRAVNADM
                     VVDGTEGSISPEKSGIIVDEDAATELILGALESLEPVSTEMPTTTEVPKIFA"
BASE COUNT         2986 a         5708 c         6319 g         3200 t
ORIGIN      
        1 gagcacgctc tcgctacatc ggtggcagca ggctgggctt gcccagcagc accgtcgtcc
       61 ctgattggtg ctagacgccg aacaggccga tgatgaagcc catgaccatc agcgtcacca
      121 gccgctcgcc ggccgagatc gccatcttcg tgaccggctg ctggtcgaag gcatcatgga
      181 cgatgaccgt cgccgccgcg atgccgaacc agagccaaaa ggctgccccg agcgcggacg
      241 ccaagtcgcc gtcgccgctg tgggcctcga tcaggctggc aacataggcc acgacgtagg
      301 cggtaagtag cgacacgacg ccggtgatgg ccgccgcgac cgacgttccc cgctccaccc
      361 gctccagggt cagtccggcc aggtgcatcc agcgattccg gaacaggtat ttcgagaacc
      421 acagccaact caccccagcg ccggcgatac ccgccagaat cactgccagc cagttgatgt
      481 cgacgtcacc catgtgtgtc tctcctcgtc cacacgtcac gccgccatga tggcgtatca
      541 atctgagcgg ggccatgcat gaacaatacc accacggacc actgttttcc gtggacccaa
      601 agaaacgtgg ccagtggcag ccttgatttt ggcaggtgct ggcagtgttc ccctcgtccc
      661 atgcaggaac catctcgttc tgtacagcca ccatgtcatt tcgaccggat cgcccgcagg
      721 gcgcgaagtg gagaaatctc tcccatgatc gaaccctgac gttcggcacg cattgagatt
      781 ccccgactgc gcggatccgc cgctccgctc gaaatgacat ggtggctggt tcacgctttc
      841 cagacgtgga tgccctccag cagggaggac acaaggtgct cccctacgag ttgtcgcgag
      901 gtccgtaggt cttgtgtttg gtgtcccggg ccattttccc gacggcggca tcgagggtag
      961 aggcgaactt ggagcggtcc ttcggcgaga acgccttcgg gccgccggtc gtctccccga
     1021 tcgagcgcag gtaggcgttg aggtcgcggg aggcgagcgc atcgacgacc cggctggagg
     1081 tgaactcggt gccgcggaag tcgaggcagc ggacgccatt gtcgaccacc ttcgcggcga
     1141 gggggatgtc ggcggtcagc accagatcac cttggaccgc acgctcggcg atccagtcgt
     1201 cggcggcgtc gggtgagccg gggacggtga ccatctcggc gtccagcccg gcgatgtccg
     1261 ggatgcggag gtagccgttg gcgacgacct tcagcggcac gctgtatcgg gcggcgacct
     1321 tgtagacgac gtccttcact gggcaggcgt cggcgtcgag gtagatgggg tgctggttgg
     1381 cttgctcgct catcatgccg aaagcgtaca ctgagctaca tctgtccctc ccagaaacgc
     1441 cacattgaaa caatctgctg gcaggagctg ctagatagga ggagagattt ctgatgcaca
     1501 tcggagcgat cttcccgcag agcgagatcg gcgcggaccc tatcgctgtg cgagatattg
     1561 cccaagccgc cgaagatctc gggtaccacc acctgctggc gttcgagcac gtgatcctgc
     1621 ctgggccgcc cgaatatcga ggctatgccg gtgcggccac cgccgaacag ccattccacg
     1681 aaccactcgt ccttttcggc tatctggccg cactcaccca gcggatcgag ctggtcaccg
     1741 atgtactggt cctgccccag cgacagaccg tgctggtcgc caagcaggcg gcggaggtgg
     1801 acgtcctgag cgggggccgg ttgcggctcg gcatcggtgt cggctggatt ccgaacgagt
     1861 ttgccgcgct cggggcagac tacagcaacc ggggaacgcg cagcgaggag cagatcgtgg
     1921 tactgcgggc cctgtggacg gaacctgtgg tgaccttccg ggggcggtgg caccagatcg
     1981 aggcggccgg catcagtcca ctgccggtcc agcgtcccat ccccatttgg atcggcggga
     2041 gggtggaggc aacgctcagg cgggtcgcgc ggatgggaga cggctggttc cccctcgatg
     2101 agcacccgga cgaccaggcc cgggagaagg tcgagcgctt gcggatgtat acggtggagg
     2161 caggccggga ccctacggcg gttggcatcg aaggctttct gagtgtctcc gccgtgccga
     2221 gagagcggtg ggcgaagcac gcggagggat ggcgtactat cggagcaacc cacctggcgg
     2281 tgagcacgat tggcgccggt tttgcctcac ctcagcagca catcgacgca ctgcagcagg
     2341 tcaaggaagt tctcagaccg taaccctgtt cgcgcagtcc ggtgcgcagg agcgcctagg
     2401 ataggaataa atcggaaagg cttgggggtg gctgcgcacc atcactagtc cttcgactct
     2461 tcgtaagact cgcgggcctc gacggtgttg cctcgctcat ctccgcggta tggacgtttg
     2521 ttcagccgga gtaccggatg ccgcgccaaa tgtccgggtg ctggaagtgg atgtcgccca
     2581 ggccggatcg ctcccaggtg gccatcagcg cgtcggtcgc ggcgtccatg atcgccgcct
     2641 cgtcgacgcc cggcaccgta cgatcccgca tccgcacctg gccgtccacg atcacggttc
     2701 gcacgtcctg cccgctcgcg tggccgacca gccgcagtgg cgctaggatg cgcggagcga
     2761 ggtgcgtctg gtgccaatcc accacgatga cgtcggcttg cttcccgacc tcgatgctgc
     2821 cgatcttgtc ctgcatgccc agtgcccttg ccgcgtcgat ggtgatcatg ccgggcgcct
     2881 tgcccgccgg cagcaaccgg tcgtcgttga agtgcacccg ctgcagctgc atgcctaccc
     2941 gcgcttggtc gatcaggtcg aacgagcgat ccggcgctga cccgtctgtc gagaacacgg
     3001 cgttgacacc cgcctccagc gcctcgatca gcgggaagcg cgccttcacg taggcgtggg
     3061 tcagcgggcc gaagctgccc gatgcaccgg tcctcgccag gatctcgatc tcctccatcg
     3121 agatcccggc gcaatgcgcc aggcaggcgt gcggtccgag cacctccgac gcgatcgcgt
     3181 gggcagcggc gatctgtccc gcgtaggcat gggtgtgtag ccccgcattg tgcttctcga
     3241 cgagcgcggc cacccccgtc acctgctggc gggagaacct gtctaccggg tcgtcggtcg
     3301 ggcagatcgc cgatgggccg acatagaact gcagcagcgg gctccgcatg tcgcgaattg
     3361 cctgaagcgc ttcttcaggt gacggcaatc gcatgctcca gatcgacctc gatctcccga
     3421 ctggagccgt ccggatggcg ctcggtgtac cggcgcggcc acggaccgtt cggcggaccc
     3481 gccgccacga tgtgccggag cccgaactca cggtagccgg tgctcgctgc gaccgcatag
     3541 cgcgcgtcgt cgacacgcgg cgaggagccg gtcatcgaca cggaggtggt gacgccgaac
     3601 cgcagccgct ggagcgcagc cagccggctc tcggcctccc agaatgcgac gtccgacgcc
     3661 tggaagtaga gatgctcgac gatcttcatc cagccgccct cgtccagccc ttcgcccaag
     3721 ccgcgcgtga ggctgtgccc ggcgtgcccg tgcgtgtcga cgatgcccgg catcacgatg
     3781 tcgcccttgg cgtcgatgac ctcgttcgcc tgctcaaggg cgctcgagtc gctctcgatc
     3841 tgcacgatac gaccatcacg aatcccgatc gcgaccgtat ccagcacacg tcggtccggg
     3901 tccatcgtga tcacggttcc gttgtgtatg gcgaggtcca gcatcagcgc atatccatcc
     3961 agtatgaacg gctcaccaca ccacccgctg ctgcttgatc gcctcgtcgt actccgcccg
     4021 ggcgtcgttg acggagggct cgtcgctgac ctcggcgatg gggacgtccc accagccctc
     4081 gtagttgggg acgcgcggct cgggatcgac ctcgacggtg acgacggtga cgcggctctc
     4141 ggtcttcgcc tcgaccagcg cctcgcgcag ctcgtcggcg gtcgtcgcgc ggatggcgcg
     4201 ggcacccagg ctctcggcgt tcgtggcgaa gtcgaccggc accggcgggc cgtccagcac
     4261 gccgccctcg ctgcggtagc gcagttcgtt gccgaacgac ggcgtcccgg agcccatctg
     4321 cagaccgtgg atgctctgga agccgtggtt gtcgaccacc accacgacga acttcacgcc
     4381 ctcctgcacg gcggtgacga tctcggtgtg cagcatcagg taggagccgt cgccgatcat
     4441 cacgaagacc tcgcggcccg gatcggccag cttcaccccc agcccggccg ggatctcgta
     4501 gcccatgcag ctgtagccgt actcgacgtg gtagcccttc gggtcacgcg cgcgccagag
     4561 cttgagcagg tcgcctggca tgctgccagc tgcgttgatc accgtgccgt ggtcgccggc
     4621 ggcctcgttg acgagcccga gcacctgcgc ctggctgagc ttctcgccgt cgccctcgat
     4681 cgcgcagagg cggtcgacct cggcctccca ctcccgcttg aggtcctgca ccggcttgcc
     4741 ggtcgcgtag atgtcgcgca cggtcccctc ggcgcgcagc acctcaccga gcgcctccag
     4801 cgtcgcccac gcatcgccgg tcaacggcac cgcgcccatc ttatgggcat ccataccggc
     4861 gacattgatg ccgatgaagc gcgcgttggg gttctggaac gcggtcttgg aggcggtgac
     4921 gaagtcgccc aggcgagtgc cgatcgcgat gatgacgtcg gcgtcgctgg cgatgcggtt
     4981 ggcggcgaac ccgccgttgg cgccgatcgg gccgatgttc cacacgtggt cccaagggat
     5041 ggcgcctttg cccgcctgcg actcgctgac cgggatgccg aactccgtgg cgaagtcgtc
     5101 cagcgcgtcg ctcgcgccgg agtagatgac accgccaccg gcgatgatga gcggattctc
     5161 gctattcgag atgaggcgcg cggcctcctc cagcgcgtcc tgcgggggca ccgggcgacg
     5221 gatcttccag acgcgctcct cgaagaagct tgcggggtag tcgaacgcct cggtctgggt
     5281 gtcctcgggc atggcgaggg tgacggcgcc ggtctcggcg gggtcgatga gcacgcgcat
     5341 cgcctccggg agggacatca gcagctgatc gggccggacg atgcggtcga agaacttcga
     5401 gaccgggcga aaggtgtcgt tggcggagac atcgccggcg agggtgtact cgagctgctg
     5461 cagcaccggg tgcggcgcgc ggttggcgaa ggtatcgccc ggcaggagca gcaccggcag
     5521 gcggttgacg gtcgccagcg cggcgccggt gaccatgttg gtcgcgcccg ggccgacgct
     5581 ggaggtgcag gcgaaggtgc cgaggcggtt ggtcgccttg gcgtaggcgg cggcggtgtg
     5641 aaccattgcc tgctcgttct gaggtcggta gtagcggagg agatcacggt gctcctcgag
     5701 cgcctgaccg atgccggtga cgttgccgtg accgaagatg ccgaacattc cggcgaagaa
     5761 ggggcgcgcc tcgccgtcgc gctcgacctt ctgcgcagcg aggtagcgca ccagcgcctg
     5821 tgccatggtc gcccgtatcg tggtcatggg ggtgctcctt cgtcggtcgt cgcggctggc
     5881 tgcctcgccc gtacgggcga tcaccatgat cgccctctcc tgcacagcgt ccttcattcg
     5941 gtcgggttgc cgccctgcgg gcggccgggc ggacaactgc ttcgcaggcc cgcccctacg
     6001 gattgggaag tgtcatgtcc gtcccagttc tatccatcag gttcggtcgc cgccggtcag
     6061 ggagtcgcgg cggccggcca tgccgttgat ccgcatctcg atatcctccc aggtctccag
     6121 cccggaaacc tgcttctcgg tccaggtgtc acgcacccag gcgtagcgcg ggtcgtccgc
     6181 cggctgcatg gtccgcgtcg cctcgttgcc agcgagcacg ttcaggtagt agccggtgta
     6241 gccgtgcgcg acgcagaagg cgtggtagcc ctccggcacc tgcagcaggt cgccgtcgcg
     6301 gatcgtccag gcgtggtcgt agcccggccc gtagagccgc tgcagcgcga agccgtcctg
     6361 cgggtggatt cggtagtagt agatctcctc gagatcggcc tcttccggca tccggctggc
     6421 gtcgtgcttg tgtggcggat agctgctcca gttgccgctc ggcgtgaaga cctcgaccac
     6481 cagcagccgg tgcgccggga agtccggcgt gatgatgtgg ctgatctgcc gcgcggcatt
     6541 gccggagccg cggatctcga tcgcgatgtc ctgcgggcga atcagcttcg gcgggaaggt
     6601 ctccgtcgcg cgggacctgc agatggcgac ctcaatgccg gtgtcggttt cgatgcggac
     6661 gtcggagccg accggcaggt agagcgccgt cggcttgccg ccgaagacgg tcccgggccg
     6721 cgatatctcc cactcctcgt ctccgaggcc gacggcggcc tcgccgacgg tcggcacgag
     6781 gcagagttcg tcgttgggag aggagaacgt gaaggtttcg ccctcaccga tgcggcgcac
     6841 ctccagcccg atgtactccc agccggcctc ctcaggtccc acctgcatca ccaggccgtg
     6901 gtcgccgggg tcgcgtggct tcatcagcag ctcattggcg ggtgtcatgc tcgctcccgt
     6961 gtgattcgag cggccattcg acgataggga gattctggca tttcgactac gggatgtcag
     7021 gcccgcgtgc agaatctctt gcaagtggcg tcacgatagt cgatcgcctg catcgacggc
     7081 cgagagttgg ccacttccga tcgaaatgac ggccggatgt tgtccgtctc aacctgtaaa
     7141 gggcggacgg cattgcgatg aggctactgg tcgaggacgg ccttgccgat gatgccggtc
     7201 gcgacgtcgt cgagcggcgg gttgtcgagc cccgcacggg catcgcggaa gtagcgctcc
     7261 agtggatgct tgcgctgcag gccggccgca ccgacgatcc gcagcgcctg gtcggtgata
     7321 tcgatcacgt tgttggtgac cagcaccttc gccccggcca actgccagcc cagctcgtcg
     7381 cgctgctccg gatggtcctc ccactgctgc accgcgccgt agagcgtgga gcgagcggcg
     7441 agcagcttga gctcgatatc cgccacgcgc tgctgaacgt tcggcaacgt ggcgatcgtc
     7501 tcgccgccca tcgccgccgg cttgcgggtc ttggcgaagg cgatcgtgaa atcgcgcgcg
     7561 gcgacgctga cgccgaggta gacggcggtg gtgaggatgc tccacgggct gttgctgggc
     7621 ttgccggaga ccggcaggcg gtggctgtcc ggcacgacga cgtcggtcag cacgaggtcg
     7681 tgactgccgg tggcgctcat gctcatgttg tcccacgtct cgtcgatctc gacgccctcg
     7741 gcgtccatcg gcacgaggaa gctggttttc tcgaccgact cgccgttttc cgtgatggtc
     7801 gcgccgacgc cggcccaccg gagggcgggc gcgagcgtcg tccaggtctt tcggccgttg
     7861 agcttccagc agccatcgtc ggtgcgctcg gcggtcgttg cgaagaaccc gccgcgactg
     7921 gggctgccga ggcctggctc gctggcggcg gtgttgtaga gtccgccctc gttgacgacc
     7981 tggtcgatca cctgcttgcg aaggtcctgc ggccactcat cgctggtggc gacgccgtag
     8041 atgtgcccga ggtgcatgca gatcgccagc gcgacggagc cgtcgccccg cgcgaggcgt
     8101 tcctgcgcca tcatcacctc gagcggggtc gcgcccatgc cacccatctc ttccggaatg
     8161 gtaagggtgt ggtagccggc gtcgatgagc gcctggaagg tgtcgtgcgg gaaggtgttg
     8221 tcgcggtcgt gtgccggggc attgcgcccg gcgatctcgg cgagcgactc ggctatctcg
     8281 atgatgctaa gctgccggcc gttctgtgga aactgcgggc cggggacggg tggcaggctc
     8341 gtctccggca gggtcgatgt caccatgggg tctccatcta cttgcatggc gcgggagcga
     8401 gcacggggct cggtcgcggt ctcctctgca tgaacctagc caccgtcgcg gacgcttgtc
     8461 aatgcgccgg ctgatggtca ctggcaccga tggtgcgaac ctgcgagcat gtgccgtggc
     8521 ggcggaatgg ttcgtcgtcc gtatggtggt gcgccggcac ggctggtgtg tcgttgggaa
     8581 ggaactcatt gatgcgtctg ttcagagtga tggcgccggt gctgctggcg ttctccatgg
     8641 ttgccggcat gacgaccgcg accgccgcgc aggatgtacc ggaggcgaca ccggagaccg
     8701 cgctacccga agggttggag aagtggtacg cgcgaacgtt cagcgccgac ctctttgcga
     8761 tgttcggccc ggaggcgagc ccggccgccg atccggaagg ctggttcctg ctgtcgacca
     8821 acgtgctgaa gttcgagacc gaggagcagg cagcagcggc cgctgaggag ctgctggcgc
     8881 agttcgagga ggagctggcc gccgaggacc tccagctgga ggatgtcgag ctggatctcg
     8941 agctcgatta cacggcgcag cgcgcggaag aggaagatca aggcctcacc actgtcgtgc
     9001 tcgaggctgt cgcgcaggac ggccagtacg tctacgccgt ggtcggtgtc acgctcggcg
     9061 tcgacccggc cccgctcatc gagcgcgcga ttcaggcgat gcaggagggc gacgtcagcg
     9121 gcgaggagga cttcgacatc gtcggtatgt cggagggcgg cctctgggcg aagctgccga
     9181 cggtcgagga gatcacggct gaggcgccag cgctggtcac ggtggaggac acgatcttct
     9241 tcccggcacc ggagctgcaa gcgacgccgg cagcgtagat ttcggaattg ctcgctgttg
     9301 aagagccccg tcccggtcac cgtgaccggg gcggggtatt ttctgtcctc aggatgactg
     9361 ctcctcggga cgatccctgt ttccagcact tgcgcagtca tatggaatgc ggctgattgc
     9421 tgagtgaacc gttggggcgg ggctcgtccg gatacccgcc cctcggaaga ccgggccgac
     9481 cactccctgc gggaggtcgg cccctacgga tgggagcgtt cgctgcatgc tcacccggat
     9541 ctcgcatcag acgttggcat gccgccagtt ttggtgtcgg ccgcgaagtg gtcggggagg
     9601 ccatcggcag cgcggatggc ggctgctgcg gtggcgatgc cgtagttgac gcgctgcatc
     9661 gttgcgacga cgctctccgg agtcacctcc agcagcagcc atccggcgcg aggatcgcca
     9721 tccttcggct tgccgacact gccagcgttg atgaagcgga catcgttgat gatgcggtcc
     9781 cacgggatgt gggtgtggcc gaagacaagc acgtcgcagt ctgcgacggc ggcgatgcgc
     9841 tccagcgagc gctcgtcgcg gtcctcgaag aggtactcgt tcatgcggcg cgaactgccg
     9901 tgaacgagtc ggatgcgatg accatgcgcc tcgacgcgga tctccggcag cagcgatcgc
     9961 aaccacgcct tgcgctcggc gtcgacctgc gctcgcgtcc acatcagcga tgcctgcccg
    10021 cgctcgcgct cgccagcgtc cctgtaggcg ccgccgcagt cgtcacggcc ggagccgacg
    10081 ccgtcgtcgt agttgcccat gatcgtcggg atgtcgcgct cacggacgag gtcgatcact
    10141 tcattggggc tggcaccgta gccgatgaga tctccgaggc agtagagccg gtcgaccccg
    10201 gcgctgtcga tgtcctccag cacggtgcgg gcggcctgga tgttgccgtg gatgtctccg
    10261 aagatcgcaa tgcgcgtcat gccggagctc cctttggagt gaagaggcgt ctgcggagcc
    10321 agagcgcaac gtagaccaat gcgatcaacg cggggacctc gatgagcgga ccgaccacgc
    10381 cggcgatcgc ctcgccggag ctgatgccga agtccccgat cgcgacggcg atggccagct
    10441 cgaagttgtt accggcggcg gtgaaggcaa tcgctgccgt ctcctcatag gagaagccaa
    10501 gccgccatgc ccagacgaag gcgatgccga acatgatgcc gaagtacgcc agcagcggca
    10561 gtgagatgcg cagcacgtcc atcggcagct cgacgaggcg gtcgccctgc atcgagaaca
    10621 agatcacgat ggtgtagagc aggcccagaa gtgcggtcgg accgatcttc ggcaggaacg
    10681 tgtgctcgta ccagtgctcg cccttcttcc ggacgagtga gaagcgggtg agcgctccgg
    10741 cgaacagcgg gatgccgagg aagatgccga cattgagggc gatgtcggcc atcgacacgt
    10801 cgagcgccgc cgagtcagcg ccgaaccagc cggggatgac gctgagaaag agccatccaa
    10861 gcaccgagta catcagaatc tggaagagcg agttgatcgc gaccagcacg gcggcaacct
    10921 caccatcgcc gcaggcgagc tggttccaga tcaagaccat cgcgatacag cgcgcgagac
    10981 cgatcaggat gagcccggtt cggtagtgcg gctcgtcggg caggaaggcc caggcgagcg
    11041 cgaacatcag caccggaccg atgatccagt tgagggcgac cgagagggag agcaaccggc
    11101 cgttggaggc tacgctgccg agcgagccgt acctcacttt ggccaccacc gggtacatca
    11161 tccagaagag gccgatcgcg atcggcacgg agacgccggc gatcttcacc cggtccagct
    11221 gctcgccgag atcgggaaag atcctgccga gcaggaggcc gatcgccatg gcggcgaaaa
    11281 tccagagcgg caggaagcgg tcgatcacgc cgagcttctg cagctgctca tcgcgctggc
    11341 ggtcgacagt ggtagtgacg gatggcgttg cggacatgtg acgttctcgt ggcgtggtac
    11401 ggagtgggcg ggcgatatac cttccgccgc ctgaagcata gatgggcgcc tatgcgttga
    11461 tgtaaccctc tgtgcgtgat gtagcagtaa tttcacattt gcggcacgga aatctcgtgc
    11521 tatagagggt tggacgaccg catatcgttg gagggcgtcc atcactgggc atgacaggat
    11581 aggcaacggt gggagagacg atttcggcgg ttctgctgga tctcgacgag acgatcctct
    11641 acgacgagtt ggccaccgag gctgcgttca gcgcgacggc gacgcatgcg gcggagatcg
    11701 ctgcagtcga ccctggttgc ctgattgtag ccgtgcagga ggaggcgcgc gcgctgtggc
    11761 tggagggccc ggaccccgcc tggtgtgatg acatcggcac gagtgaggtc gaagggctcc
    11821 gcgcccggtt cgaggggaac gacccccgga ccgttgccat gcgggcctgg ggaccggggt
    11881 ttcggagcga gagctggaaa cgtgcccttc gccgttgcgg gatcgatgac gacatgttgg
    11941 caagcgagct cgacgcccgg ttcgagcagg agcgcgtgtc gacaaacccg ttcatccccg
    12001 gcgtggagga gacgctcgat cagttggagg agcgataccg gctcgcgatc gtgaccaacg
    12061 gcctgccgga cgtccagcgc gagaagctgg tccgcacggg gttgatggac cggttcgacg
    12121 tggtggtcat ctcgggcgag ctggggttcg gcaagccgga tcggcgcatg tatgacgaga
    12181 cggtgaggca gcttaggctg atagctggcg aatgcgtgat ggtcggcgac aatttccggc
    12241 gggacgtcgc cggcgcgcag gaggccggga ttcggggcgt gtggatctcc tgcgggaagg
    12301 acctgccgga caccgatgtc acaccgttcc tggtggtgga gtcgctggcc gagctgcctt
    12361 cgaagttagc atcgtgtagg ggctgacctg tgcggtggtg gtcggcccgc ctacagacgg
    12421 gagcaacatg ggcggacaac tccctgcggc aggtccgccc ctacggattg aagtggatgg
    12481 cgcagactgc tgctcggtta gatgtaaaca gccgctcgga gagaaatcct ccgggcggct
    12541 gtcgttgcgc gtgatgatgg caactggcgg gcgctagacg cgctgctgct gctcggtgcc
    12601 catcttcgac atctcggaca tcttgatctt gaggccgtcg gcgatacgct ggccgaactc
    12661 ctcgtcgcac ttggtgaagt gctcgaccat catgtcctgc aggacctggt cgcactggct
    12721 gagcccagtg accaggttca gcaccaggtc atcgcgctcc cactgctcaa tggtgcggta
    12781 gcgctcgccg gcctgcttgt agttgttggt ccggtcgatc ggctccttga cgacattgcc
    12841 gtcaacgtag ggctggtaga gcgtgtagga gtcgtccgcc tcggagagac cggcgagcac
    12901 cgacggctcg tagttgaccg actggtcggc gccgcgcttg tcgaccccat aggacatctg
    12961 gccgccctcc tggttggtgc gcgtttccac caccggccgg ttgaccggca gttgcaggta
    13021 gttcgggccg acgcggtagc gctgggtgtc gctgtaggag aaggtgcggc cgaccaacat
    13081 cttgtcgtcg gagaagtcga ggccgtctac cagcaccccg gtgccgaagg cgatctgctc
    13141 gttctcgtcg aactggttct tcgggttctc gttgagcgtc atcatgccga ccgggagcag
    13201 cgggaactgg tcctccggcc aggtcttggt gtcgtccagc gggtcgaagt ccagctccgg
    13261 gtgctcgtcg tcgctcatga tctggacgtt gagctcccac tgcgggtagt cgccggcttc
    13321 gattgcgtcg tagaggtcct tggtgtggct gccgagcgtc ttcgcctgga tctcggcggc
    13381 ctcggcagca gtcagcgact tgatgccctg ctggctcttc cagtggtact tgacgaggac
    13441 agcctcgccc ttgtcgttga ccatacggta ggtgttgacg ccgaagccgt cctggtgccg
    13501 atagctggcc gggatgccgc gcggcgagaa gagccacgtg agcatgtgca tcgactcggg
    13561 cgacgcggcc cagaagtcga acgcgcgctg cggcacgttg gcctcgttgg tgaccgggtc
    13621 gggcttctgg gagtggatga agtccgggaa cttgatcgcg tcgcggatga agaagaccgg
    13681 caggttgttg ccgacgaggt cccagttgcc gtcctcggtg cggagcttga ccgcgaaacc
    13741 gcgcgggtcg cgggcgacct cggaggagtc gcggccaccg gccacggtcg agaagcggac
    13801 ggtcaccggg gtcttcttgc ccttctcttg gaagagcttg gcgcgggtgt aggtgctggc
    13861 cggctcgtca ccgatggtgc cgtaggcttc gaagacgccg tgggcgatgg tgcctcgcgc
    13921 gtgaacgaca cgctccggga tgcgctcacg gtcgaagtgg ctgatcttct cgaggaactg
    13981 gtagttctcc atcgtggccg ggccgcggtt gccgaccgtg cggaggtcct gattgttgtg
    14041 gaccgggtgg ccctgccgtg tggtcagggc cggcttgtcg gcctgcggct tcctgttcgt
    14101 cttcccattg gacttgccgt cgctcatcgc gatgctcctt taaaagggtc gctggccgtg
    14161 ccgcgtccat gccggaagac cgcatctggg ttcgtgagag aatcagggaa gggtcggaac
    14221 cggaggggta gggcgaggtc agcgcacact gcacagactt gggacattgt atatgaatcc
    14281 gccgagggag gcgcgtatgg catcccccaa ggcggaggtt cccgggtagc tcgcaggtgg
    14341 cgtgccgggc atcggggcag gggacgtgat catgatgagc gagcggatac aggtgattct
    14401 ggacaccgac atcggcaccg atgtcgacga ctgcttggcg ctggcggtgc tgctggggtc
    14461 acccgagatc gacctcgtcg gcgtcaccac ggtctacggc gacgtcgccc tgcgggcgcg
    14521 gatggtgcgc aagctgctgc acctgcgcgg ccgagatgag gtgccggtcc acgaaggggt
    14581 gcgtgagccg ctggtgcgca atcggcaggt ctactggcca gggcacgagg gggtcgggct
    14641 gctggaggcg ggcgacgacg agcggttcgg gcaggggccg ggccaacatg cggtcgactt
    14701 cctcatcgag cgggtgatga ggcaaccggg cgaagtgcat ctgctcgcgg tcgggccgct
    14761 ggccaatgtt gcagctgcga tgatccggga gccgggtctg gcgagcaatc tcgtacacat
    14821 gacgatcatg ggcgggtaca tcgcgccgtg gaccggcggc cggggtacgg cagagcacaa
    14881 catcctcagt gatcccgagg ctgcacgggt cgtgtttgcg tctggcgcgc cgatctcgct
    14941 ggtgccgctc gacgtgacgc tgaagacggt gatcacgcga gaggacgtgg ccgagatccg
    15001 ggaggtcggg acactgttcc acgacgccgt cgctgaccag gtgatgctct acccgccgtt
    15061 cgagaatcgg ggcgggcaga ccttcctcca cgaccccctc gccgcggcgg ggatcgtccg
    15121 gcccgacctg ctggagtggc acgagttggc ggtcgacatc gagctggcag gccggctgac
    15181 gaccggcatg acggtggcgc ggcaaccggg accacaggcg cccgccacgg cacgagtcgc
    15241 gttgggggtt gatgtgatcg aggcacagcg gttcatcctc gatcgggtgt gcgcccgtca
    15301 ataggaggcg accgcagggc tcctacgcac cccttcgtgc tggtcgattc cggataccac
    15361 gaagccgtgt cattctgagc cggcgagggg cctcagaatg acacgattcc agtgcgccgc
    15421 gtcgaatcca gcggaggacg gttgctacga ccgctgcttc tggccccact tgccgtagct
    15481 gacgagctcg tcgagcggct tgcggccggg cttcgggccg gtcggtcgtg gatccttcga
    15541 cgtcctgtag gggccgagca cgacgaccga gtggagcacc atgtcgtccg ggaccccgag
    15601 gatctgcttg gcgacgccct cgttctcggc aaaccacgcg gtgccggcac cgaggccgag
    15661 catctcggcg gcgatcatga cgcgctcgct gacacggccc tcgtcgtaag cctcgtggat
    15721 gtcgttctcg ccggggaaga caagggcgat cgcgagggcg ccattggcga cccaggtgat
    15781 gttcggccgc aattccgcga ggcgcgcgag tacgtccttg tcctcgacaa tgacgaagcg
    15841 ccacggttgg gtgttgcgcg agctgcccga ccagcgagcg atctcgagaa tcctgttcag
    15901 gtcctcgtcg ctgacagaac ccggttcgta ctgccggacc tggcggacct tgcgaatcgc
    15961 ctccaaggca tcggcggtgt tggtgatctc agccatcgtg ttgcctcctg tcatggtcgg
    16021 tgtggtcggc ctgtccctgc atcatagcgt cacgggacga gacggcaaat atccggccat
    16081 cgctcacgcc atatctgggg agacaaccag ggaatcgaca tttgggtgaa ctgccgagaa
    16141 cacgctaatg cccgacgccc tgatagcggt tgagggcctc accccatagc caagcggtga
    16201 tggcgacgac gagtgtcgcc accagcggcg tggcgagacc gagccatcgc catggggcgt
    16261 cacggtcgag gagcaggagg gcggggaagt agctgatgaa ggcgtagggg atcaccaccg
    16321 ttaccagacc ccggatcacc agattgaaga cgtcgagcgg gagcttcgcg aagtcgatca
    16381 tgagcgcgac cagcgtgggc agcgcgctct gggccgacgg ctcccagaaa ccgatcatgt
    16441 tgatgacgta gttgagcgca ccgatcatca ccgcgctcgc ggcgatcgtc accagcagga
    16501 agggtagcgt ccaccaggta acgtcatctc cgagcgactc atgccgatcc acagcacgat
    16561 gatgccgagg ccgagctgcc cgaagccgtg gatggaggcg atcgccgtgg cggactgcaa
    16621 cgccggggaa atggggcgga cgagcacccg gtcgaactcg ccggtgttca cctgcgttcg
    16681 cagcatccgc gggccgtcgc agagaaactc gatcaggccc ttcgggatca tcgcgagacc
    16741 gtagagaatc gccacgttcc aggcggtcca gcccccgact tcggggatct gggtgaagag
    16801 ggcggcgatg aagacgagtc cggccgcctg ctgcagcatc gcgccgatga tcccgatcca
    16861 gaagtcgcca ctgtactcaa ccgcggcccg aagctgagcg agctggaggg tgaggtagag
    16921 ccgagcgttt ctgcgtacct gcgcgatcga cgggatggcg atgttcaccg gtcaccctcc
    16981 ctggacctcg acggcgtcga agaccgcgcg ccacgcccag tcggcgaaga cccacagcgc
    17041 cgccaaccag acgagctgca tgccaagcat ctggacgacc gtccatccct cggccttgcc
    17101 gaggtagagc gtcactggcg tgtagacgat gccgcgcatg ggcagcacgt gcggcgccac
    17161 gcaactgatc cattcctccg cgcgtatacg tcactaggaa cggttccgcg cagtacgtct
    17221 tcgtgtgtgg ctcgatcggc agcccatgtg gtagcacggg gcagcggacc tcctgacagc
    17281 aagccaaggg tgagagcggg cgtccgcacc ccgacagcac gccgagcgat cgtgcagggg
    17341 atcgatgcgg ccttggaccg gccgcttcga tgtgtgcccg tgaagtgggg aagaacgcag
    17401 atcgatggtg caacagacct ttcctgacgt ggcggaggca tcggacggcg cgccgatatg
    17461 ggaatccatg ttcggccgga taaccgattt tctgcgcggg gtcgtgctgc cggacgagca
    17521 gcacgcgccc atccggatca cgagcacggg cgactcggtg ctcgacggcg acgatcccag
    17581 ccccatgggg cgcctcgggc ggctgatccc gaggctgctg ctggtgctcg ccgtggcgct
    17641 gcttgtggcg gcgatcgcgc tctttggttt ccacgccgcc tacgccgaca aggtctaccc
    17701 ggcgatcgct gtcggcgacg tcagtgtcgg cggcatgacc gttgagcagg cggtcgccac
    17761 cgtcgagcag cgcgctgccg agttcgagcg cggcaccgtc accttcacct acaacgggca
    17821 gacatggact ccgacgctgg ccgagctcgg agcatcgatt gacgtggagt cgtcggtcga
    17881 cgccgcctgg gagctcgggc gcgacgagag tgcagtcgat cgtctcggat tcacccgaca
    17941 gctgctgcgg agtgaccagc gggtaccgct gcgcaccacg gtcgacctta ccgtattggc
    18001 gggctggttc gaacaggtca acgccgatat cgaccagcgc gcggtgaatg cggatatggt
    18061 cgtggatggc accgagggca gcatctcgcc cgagaagagc ggcatcattg tcgacgagga
    18121 cgcggcaacc gagctgatcc ttggcgcgct ggagtcgctg gagccggtgt cgaccgagat
    18181 gccgacgacg accgaagtgc cgaagatctt tgc
//
LOCUS       DASYWL010000084        15289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_210183, whole genome shotgun sequence.
ACCESSION   DASYWL010000084 DASYWL010000000
VERSION     DASYWL010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 15289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15289
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210183"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..1686)
                     /gene="pyk"
                     /locus_tag="VGR22_03165"
     CDS             complement(232..1686)
                     /gene="pyk"
                     /locus_tag="VGR22_03165"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEV2127600.1"
                     /translation="MTQRARTKVVATIGPATESPKQIAAMIEAGVSVFRLNFSHGTRE
                     THRRVYHTIREAAEQAERVTGILQDLQGPKLRIGELKDGSPFTLEQGETLRICTRPIE
                     GTPDMVSTSYDRFGEDLRVGDPVLIDDGRLRFGVDSIERNTDEYGDIVTLTVLQAGTL
                     SPRKGINLPETNVSAEALTEKDRADLEFGVELGVDMIALSFVRSADDIRTAKAAITEA
                     GGTQPLIAKIEKPQAVRVLQQIVEAADGVMVARGDLGVEMPSEGVPLIQKQIIRAANV
                     AGKPVITATQMLESMQQNPHPTRAEASDVANAILDGTDAIMLSGETAVGKYPIRAVET
                     MIRIASSVERERLGTPWQLRREGIRSYHNQTESKAVGHAARALADDLRAKAIVVLTRT
                     GETAQNVSEERPVEPIIAFTDNPVVGRRLALAYGVVPLVMPLRQTIDELIVQVNREIE
                     RRDYASTGDRVVIVGAAPRRNEHPSVFIEIHTMS"
     gene            1859..4090
                     /gene="ppk1"
                     /locus_tag="VGR22_03170"
     CDS             1859..4090
                     /gene="ppk1"
                     /locus_tag="VGR22_03170"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003918.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEV2127601.1"
                     /translation="MSPKQAKGKSAPERFEAFLDERPSLTPDLFINRELSWLEFNRRV
                     LEEAAEKDLPLLARTRFAAIFAANLDEFFMIRVAGVKRKVMAGIGDYGPDGRTPVQQL
                     REIHKVTQKLLTEHCRLVHQELLPRLASQGVAIERYDDLEEDCQQALRKVFEQEIFPV
                     LTPQAVDRGRRFPHVSNDSLNLIVVLRGDDGSRFARVKVPALLPRFVPVPSTAPEGET
                     GKVLADDNMHASTRFTWLEDIIAANLHRLFPGSDIIAAYPFHLLRDSDVEPDEDDDGH
                     DDLMEIMRETLSQRPFGTTVRIDIDRHMPEEVRDWLMDQVRATERDLYVVEGALQLED
                     LSELQELDRPDLDEAIFAPAPVFGARVESTDGPDAPLGVFRLIRENDILLHHPYQSFN
                     AVMDFVRAASIDPDVVAIKQTLYRIGKDSPLIPALITARDEDTQVAVLVELKARFDEE
                     DNISWAQTLERAGVHVAYGLSGLKTHCKVTLVVRREADGLRRYVHLSTGNYNANTARV
                     YEDIGFLTSRDDIARDASELFNELTGYGEQEVYDTMWVAPRDMRRQLLEGIYQEVLRH
                     REDGNGHVIFKMNSLVDREVIRALYAASSEGVRVELIVRGACCLRPGVKGFSETIHVR
                     SIVGRFLEHSRVYWFHNGGEPKVYLGSADMMERNLDRRVEVIFPVDSPELKDHLRDTV
                     LASYLRDTANARELREDAVWRPVMPGKGEKPFGVHQWFMRFYRDHHIVGPRRERAQEP
                     APT"
     gene            complement(4087..4941)
                     /locus_tag="VGR22_03175"
     CDS             complement(4087..4941)
                     /locus_tag="VGR22_03175"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV2127602.1"
                     /translation="MSILALSLILASAVIHATWNLFAKRLSGGGETVWLFTAAAAALY
                     APVAAVTIAVTGYQPGGADLVFLLGTGVLQTLYFVVLRRGYATDDLSIVYPLARGTGP
                     LVAVVLATALVGERPSPLTAAGAGVVALGTLLLVTPVSRQSDHRKGALLGMATGVIIG
                     CYTVWDGYAVGSLAIPALLLAWAGDAGRMVLLTPLALRRRMLLSTQICNHRRELLAVA
                     VLSTLSYVMVLAAMEIAPISSVAPARELGIVIGALFGMLLLGEPGGWRRVSSAAVITA
                     GVGLVALG"
     gene            5055..5879
                     /locus_tag="VGR22_03180"
     CDS             5055..5879
                     /locus_tag="VGR22_03180"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127603.1"
                     /translation="MIFNSPDRIERLTPNSPFGRREDGRPDTPSDLLERLKLVTNDEA
                     WGVLERRHGYHFQFEGGWPVHLQPDVPLTGRAVTAQYVPLRPDLNEVVQEIGTGEGRS
                     GGQNTWIIDQLQPGDVLVVDLFGKVEDGTFIGDNLATAARARTGTGMIIDGGIRDSLR
                     VNQLTDFPVYCRGIHPSAIYDVTLTAANVPIRVGDATVMPGDAVLGTRAGVSFIPPHL
                     LQEVVEVSEDTRQRDVFGKQRLAEGTYTSGEIDVSVWADHIEADYQEWAKANGSGA"
     gene            5876..7165
                     /locus_tag="VGR22_03185"
     CDS             5876..7165
                     /locus_tag="VGR22_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEV2127604.1"
                     /translation="MSTRHIEPAIPPGWERFRGEDGVTIRDVRVILTAPDNIRLVVVK
                     VETSEPGLYGLGCATFTQRPLAVKTAIEEYLRPFLIGRDVHDIEDIWQASFVSSYWRS
                     GPVLNNAMSGIDMALWDIKGKVANLPVYQLLGGKVREAADVYVHASGSEPEEVVERCQ
                     ELLEEGYRHLRCQVAVTGYATYGVYSKKQDRTYAVRIDTHQDRWSPEAYLRRVPAMFE
                     QVRDALGSDVELLHDIHERIPPMHAIRLAQELDQYRLFFLEDPFPPEDNGWFPMLRQH
                     SVTPIAMGELYVNQAEYLPLIEGRLIDFIRVHLSDIGGLTPARKLAALCEFYGVRTAW
                     HGPGDVSPVGHAANLALNLSSSNFGIQEATFFTERAREVFPGTPELRDGALWSNDRLG
                     LGVDVDEELAAKYPFPDHPVNGAWPEIRRVDGTVQKP"
     gene            complement(7233..8876)
                     /locus_tag="VGR22_03190"
     CDS             complement(7233..8876)
                     /locus_tag="VGR22_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV2127605.1"
                     /translation="MFSLSSVSYRAGDTLLFDQLTLTINRGDRLGIIGPNGAGKSTLL
                     RIMAGVCAPTSGTRTLAPGARIGYLPQGILDLETGTLADALDTASGGYFGTRRELDLA
                     TEALSEPYGASEIVLDRWEQAQAAFEASGGYDIADRLEAHFARFDLPPDAPERPLSQL
                     SGGERTRAALATMLAVRPDLLLLDEPTNHLDVAAQRWLAKFIADYPGAIVIVSHDRAF
                     LEETVTHIAAFETGSSTVELHTGGYEDWVETRRRRAATEFETWKRQREKIAGLQASID
                     QQERSSRKVESETIHFHYRKRAAKVARAATVRRARLERMLESEERAEKPKPQWGVALQ
                     FPEPDVRARDVVTLDEVMLQRGGHRILDNVTLTLRYGDRLAVVGENGAGKTTMLRVIS
                     GELEPDTGYRRLAPGVSIGTLAQDQDTLDPALTVLESVQKCLAASEAALRTELHRYLF
                     GGDAVHRRVADLSFGERSRLMLALIAIPGADLLLLDEPHNHLDVDARAAFEAAVQAFP
                     GTVVLISHDRYAIRQIATRVMRIAEGRLQEIDASMDHEF"
     gene            complement(9031..9291)
                     /locus_tag="VGR22_03195"
     CDS             complement(9031..9291)
                     /locus_tag="VGR22_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127606.1"
                     /translation="MDTYNLYMDEIPADGGDDAETVDVEFRVVPASEDDSDDENTPVV
                     AGLDLVDLINLRDALSQEIDNYALTALEAEATAALGGDEAAG"
     gene            9625..10797
                     /locus_tag="VGR22_03200"
     CDS             9625..10797
                     /locus_tag="VGR22_03200"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV2127607.1"
                     /translation="MAAGAGLALGGCSPLDGEEDVAEEVVTAPAELVDDVALATQAPP
                     TPTQAPVASAVPGYLDPERWYARSLVVATAGVGDYLEALTAAFFEPFSEATGVTVQHQ
                     AFGRDGIANLVDQVENGEVVWDVVLIPTGDVLPLAQHGYLEPIDYAIVDSTSLYEPAL
                     MQHGVGAMYYSTVMTSSAALEDEPDSWDDFWDLMTFDGTRALRRYPVGTLEFALLAAG
                     VPQDELYPLDVPLAFEMLDLIREATIFYEDNKQPVELVRTGQVGLASAWNIRAVLPDV
                     TSLVNLHWQGGMISADSWAVPRGTENLDVAMGFIDFATRAVPSANFSLYLPFGPANRD
                     ALELIRPDVVEVLPTGPAHLEVQFFENWAYWADNRDELVAQFEDWLLNPTASPTEG"
     gene            complement(10794..12368)
                     /locus_tag="VGR22_03205"
     CDS             complement(10794..12368)
                     /locus_tag="VGR22_03205"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEV2127608.1"
                     /translation="MPSTTAPTAMIATPHHVASTIGVDVLLEGGTAADAAIAANAALT
                     VLLPDQTSIGGDCFFQIWEPGEEGPLGFNGSGRSAAAANADVVRNAGFSGMPRRGPWT
                     VTVPGTIDAWHQAHRRYGSLPMERLLEPAIELARHGFEISPRLSAAIAAEVNDLAQSP
                     LTAAIYLDGGFAPQPGFWLVNGGLADTLERIAREGRSAFYRGEVAAAIVEAVGGGDGW
                     MTVEDLATHRGEWVTPVESRYRDATVWELPPNSQGVTAQIGLEMMERMPFPGTWDDPA
                     WLHVEIEAKKRAFSIRDRYMSDPAAMTVDVQAYLDAGTIDQLWAEFTPAMATTGQVTL
                     PGDTIYLAVVDETGLMVSLIQSIYSGFGSAVIAGETGVLLQNRGSYFSLVPGHANELA
                     GGKRTLHTLMPGMIEIPGRFRGPFGTQGGDAQAQVHMQLVSHLVDSGATPGEAIARPR
                     WVSGDARLDPFTVVVEAGMEWHVIAGLQERGHQVAQVEPGWPHAGYAQMVLRQPETGE
                     LVGAADPRAEGSVEGY"
     gene            12480..13601
                     /locus_tag="VGR22_03210"
     CDS             12480..13601
                     /locus_tag="VGR22_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127609.1"
                     /translation="MHPRRILRGTVLVLLASLTMFAVAGGSHAQVADDTRAALAAIAL
                     TAAEMPSGYELVGEAFVGPDGGPIAGEVPAVLTDAGFLGAYMSVYENADGTVTITSYA
                     SAWTDADAAETAFALLETEDAGETDESLDAGDGPAELTVVGETMDATFVVDRFVVGVA
                     ATGSDIDRDDVMALVTLLESRANAVADGQFPAGTDAALPASVLDTSALGTQVRAGYLS
                     ATESEQLYGLTGSALGGMQASWVSLVTVGDGGEPPYVAIAASNFESADNAARVVDQAE
                     DLVPLTVDLQPVEGITVEDADTARGFQYSSTMSGTTDTPDSVRVVAQVGDQVVVVDVQ
                     GATSAEEVQAAAVSLANAQVACMMEEGNCELPEVTLGGA"
     gene            complement(13778..14056)
                     /locus_tag="VGR22_03215"
     CDS             complement(13778..14056)
                     /locus_tag="VGR22_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127610.1"
                     /translation="MSIRKPSATTGRPGPGKTDRRDGHLRLVALVTGVAMALGVLVGC
                     SEGTSQDAERGQQQDARRTSVVSDLQATRSARVLEPGTPPPLTATATP"
     gene            complement(14001..15023)
                     /locus_tag="VGR22_03220"
     CDS             complement(14001..15023)
                     /locus_tag="VGR22_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV2127611.1"
                     /translation="MTPSTGTDNLTIAAAVDIFFGDLRLAPRSRTTYRIGIEKFLRHL
                     ARHEGIDPVTAPVEALTVEHVTNFASVLVPDDIRTPEEVSAMRTAQNTLAAVRKLCSY
                     LSAYDLHPDLSTDRIRVRVAAMMPRFTPPPPDVSLEDLEAIVAHVSSRPREEKPHLEL
                     RRLKVQALILFMYRTGVRVSELCAMRRRDISLDDGAAHIYRAKGGKSRTVHFDADTAH
                     ALSAYWKARGDQPRGAGANPAFSGRDKVGVAGKQISPRTVEHIVSEICNEVGIESVVT
                     PHSFRHGLATELVRRRVRESTVQTILGHASPQTTRIYVHKSAVEVADEYQEAFGDYRP
                     PRTGQD"
BASE COUNT         2670 a         4777 c         5070 g         2772 t
ORIGIN      
        1 gggcggtcgt cttggcggcg gcaatcagag tgcgggccac ggcgggcctg caagcaggct
       61 cgccgtggct gggcactgcc gttcgtgaca cgagaaatct ctttactccg caccctacag
      121 gagtgagccc gccccgatcc cagcgcaagc ggagcatcct gggttttcca gtgaatactt
      181 cgtacatggc tgtcgcccat agcgagcgca gggatgtatc ctggcggtga ttcagctcat
      241 ggtgtggatc tcgatgaaga ctgacgggtg ctcattccgg cgcggggcgg ccccgacgat
      301 caccacccgg tccccggtcg aggcatagtc tcgccgctcg atctcgcggt ttacctgcac
      361 gatgagttcg tcgatcgtct gtcgcagcgg catcaccagt ggcacgacgc cataggcgag
      421 ggcgagccgt cgccccacca ccgggttgtc ggtgaaggcg atgatgggct cgaccggccg
      481 ctcctcggag acgttctgtg ccgtttcccc cgttcgtgtg agcacgacga tcgccttcgc
      541 cctgagatcg tcggccaacg cgcgcgctgc gtggcccacg gccttggatt ccgtctggtt
      601 atggtagctg cggatgccct cgcgccgcag ctgccatggc gtgcccagcc gctcgcgctc
      661 aacggacgag gcaatacgta tcatcgtttc cactgcccga atcgggtact taccgaccgc
      721 ggtttcgcca gacagcatga ttgcgtcggt gccgtccaga atggcgttgg cgacgtcgct
      781 ggcttcggca cgtgtcggat gcggattttg ctgcatcgat tccagcatct gtgtggccgt
      841 gatgacaggc ttcccggcca cattggctgc ccggatgatc tgcttctgga tcagcggcac
      901 cccctccgac ggcatctcga cgccgagatc accacgtgcc accatcactc cgtccgcggc
      961 ctccacgatt tgctgcagca cgcgcacggc ctgtggcttc tcgatcttgg cgatgagcgg
     1021 ctgcgtgccc cccgcctcag tgatggcggc cttggctgtc cggatgtcgt cggcagagcg
     1081 cacgaagcta agcgcgatca tgtccacacc gagctcgaca ccgaattcca ggtctgcgcg
     1141 gtccttttcg gtgagtgcct cggcggagac attcgtctcc ggcaggttga tgcccttcct
     1201 cgggctcagc gtacctgcct gaagcaccgt cagggtcacg atgtcgccgt actcgtcggt
     1261 gttccgctcg atggaatcga ccccgaaacg cagcctgccg tcgtcgatga gtactggatc
     1321 tccgacccga agatcctcgc cgaagcgatc gtacgacgtc gataccatgt cgggcgtgcc
     1381 ctcaataggc cgggtgcaga tgcgcagtgt ctcgccctgc tcgagtgtga atggcgagcc
     1441 atccttgagc tcgccaatgc ggagtttggg accttgcagg tcctgcagga tgccggtcac
     1501 ccgctccgcc tgctccgccg cctcacgaat cgtgtggtaa acgcgtcggt gcgtttcgcg
     1561 agtcccatgc gagaagttca gccggaagac cgagacgccc gcctcgatca tcgcggcgat
     1621 ctgcttaggc gactcggtgg caggaccgat cgtggctacc actttggtac gggcgcgctg
     1681 agtcatcgag gatcctcctt cgcgttctga aggtggtatg ttccggttga tgcagaccgc
     1741 attctgtctt cgattgtacc catcgtgttc gctcaacaca tctcaggcgg caggccgcat
     1801 aggctatgaa ggacgcgtgc agggccgggg agtcatggcg tgcgccagga ggatgatcat
     1861 gagtcccaag caggcaaagg gcaagagcgc ccccgagaga ttcgaggcgt tcctggacga
     1921 gcgtccgtcg ctgacaccag acctgttcat caatcgcgag ctcagctggc tcgagttcaa
     1981 ccggcgtgtg ctggaggagg ccgcggagaa ggatctgccg ctgctggcca ggacgcgctt
     2041 cgctgcaatc tttgccgcca acctcgacga gttcttcatg attcgggtcg cgggggtcaa
     2101 gcgcaaggtg atggcgggga tcggtgatta cggtcccgat ggcagaactc ccgtgcagca
     2161 gctgcgcgaa atccacaagg tcactcagaa actcctgacc gagcactgcc ggctcgtgca
     2221 tcaggagctg ctgcccaggc ttgcatcgca aggcgtcgcg atcgagcggt acgacgacct
     2281 cgaggaagac tgccagcaag cgctccggaa ggtcttcgag caggagatct ttccggtgct
     2341 cacgccgcag gcggtcgatc gtggccgacg atttccgcat gtctccaacg acagtctcaa
     2401 cctgattgtt gtgctgcgcg gtgacgatgg ctctcgcttc gctcgagtga aggtgccggc
     2461 gctgctgccg cgattcgtgc ccgtgccttc gaccgcaccg gaaggggaga cgggcaaggt
     2521 gctcgccgac gataacatgc atgcgtcaac ccgcttcact tggcttgaag acatcattgc
     2581 ggccaacctt catcggttgt ttccaggcag cgacatcatc gccgcctacc cgtttcatct
     2641 gctgcgagac tcggacgtgg agccggacga ggatgacgat ggccacgatg atctgatgga
     2701 gatcatgcga gagacgctct cgcagcgccc gtttggtacg acggtccgca tcgacatcga
     2761 ccgccacatg ccggaggaag tgcgtgattg gctgatggac caggtgcgag caacggagcg
     2821 cgacctctac gtggtcgaag gtgccctgca gctggaggac ctctcggaac tgcaggagtt
     2881 ggatcgcccc gatctcgacg aggcgatctt tgcaccggcg cctgtctttg gagcccgcgt
     2941 cgagtcgaca gacggccctg atgctccgct cggtgtcttc cgcctgatcc gcgaaaacga
     3001 catcctgctg caccacccgt accagtcgtt caacgcggtc atggattttg tccgtgcagc
     3061 atcgatcgac ccggatgtcg tcgccatcaa acagacgctc taccgtattg gcaaggactc
     3121 gccgttgatc ccggcgctca tcaccgcgcg agacgaggac acgcaggtag ccgtgctcgt
     3181 cgagctgaag gcgcggttcg acgaggagga caacatctcc tgggcacaga ctctggagcg
     3241 ggccggggtc catgtcgcct acgggctcag cgggctcaag actcattgca aggtgacgct
     3301 ggtggtccgg cgcgaggcgg atggcctgcg gcgctacgtg catctctcga cgggcaacta
     3361 caacgccaac accgcgcggg tctacgagga tattggcttc ctcacctcgc gagacgacat
     3421 cgcgcgtgat gcctccgagc tcttcaacga actgactgga tacggcgagc aggaggtcta
     3481 cgacacgatg tgggtcgcac cccgcgacat gcgtcgccag ttgctggaag gcatctatca
     3541 ggaggtgctg cgccaccgcg aggacggcaa cggccacgtg atcttcaaga tgaactcgct
     3601 ggtcgatcgc gaggtcatcc gggcgctcta cgctgcatcg agtgagggcg tgcgcgttga
     3661 actcatcgtg cgtggtgcct gctgcctccg ccccggcgtg aagggtttca gcgagacgat
     3721 ccatgttcgc agcattgtcg ggcgattcct tgagcacagc cgcgtctact ggttccacaa
     3781 tggtggcgaa ccgaaggtgt atctcggcag cgctgacatg atggagcgga atctcgaccg
     3841 acgcgtggag gtgatttttc cggtcgactc acccgagctg aaggatcacc tgcgggacac
     3901 ggtattggcg agttacctcc gggatacggc gaacgcgcgg gagttgcgag aggatgcagt
     3961 ctggcgaccg gtgatgcccg ggaagggcga gaagccgttt ggcgtgcacc agtggttcat
     4021 gcgcttctac cgcgaccatc acatcgttgg tccgagacgg gaacgagcgc aggagccggc
     4081 gccgacctag ccgagcgcca cgaggccgac cccggcggtg atcacggctg ctgaactcac
     4141 ccggcgccac ccgcccggct cgccgagaag gagcatgccg aagagtgcgc cgatgacgat
     4201 gccgagctcc cgcgccggag ctactgagct gatcggcgcg atctccatcg ccgccagtac
     4261 catcacatac gacagtgtgc tcaggactgc caccgcgagg agttcgcggc ggtggttgca
     4321 gatctgtgtc gacaggagca ttcgcctccg cagggcgagc ggcgtgagca gcaccatcct
     4381 gccggcgtca ccggcccatg caagcaacag cgccggaatt gccagggagc caacggcgta
     4441 gccgtcccag accgtgtagc agccgatgat gacgcccgtt gccatgccga ggagcgcgcc
     4501 ctttcggtgg tcggattggc ggctgacggg agtcacaagg agcaacgtgc cgagcgcgac
     4561 gacacccgct ccggcggccg tcaggggcga gggtcgctcg cccacgagcg cggttgccag
     4621 cacgaccgcc accagcgggc ctgttccgcg cgccagtggg tagacgatgg agagatcgtc
     4681 ggtagcgtaa ccgcgtctca gcacgacgaa gtagagcgtc tgcagcacac cggtgccgag
     4741 aaggaagacc aggtcggcgc cgccaggttg atagccggtg accgcgatcg tgacggccgc
     4801 gacaggcgcg tagagcgccg ccgcggcggc cgtgaagagc cagaccgttt cgccaccccc
     4861 tgacaggcgc ttggcgaaaa ggttccacgt cgcgtggatg acggccgacg ccaggatcag
     4921 gctgagggcg aggatgctca cgctggtcgg ctgctctcct tgacgatcag gggtttccgg
     4981 aattggattc gcggctatag tgagtcagcg aatgcgagtt gtccagatgt ggtggcgcgc
     5041 ggggaggaat aacgatgatc ttcaacagcc ctgatcggat cgagcgactc acgccgaaca
     5101 gtccgttcgg tcgtcgggag gatgggcgcc cggacacgcc gagcgacctg cttgagcggc
     5161 tgaagctggt taccaacgac gaggcgtggg gcgtgctgga gcgacggcac gggtaccact
     5221 tccagttcga aggaggctgg ccagtacatc tgcagccgga tgtaccgctg accggacgcg
     5281 cggtcaccgc gcagtacgtt ccgctccgcc ccgacctgaa cgaggtggtc caggagatcg
     5341 gtacgggcga gggacgatcc ggtggccaga acacctggat cattgatcag ctccagcccg
     5401 gggacgtgct cgtggtcgat ttgttcggaa aggtcgagga cggcacgttt atcggtgaca
     5461 accttgctac cgcagcgcgg gcgcggaccg gtaccgggat gatcatcgac ggcggcatcc
     5521 gcgactcgtt gcgcgttaac cagctcacgg acttcccggt ctactgccgt gggatccacc
     5581 cgagcgcgat ctatgacgtc acgctgacag ccgccaacgt gccgatccgc gtaggtgacg
     5641 cgaccgtgat gcccggcgac gccgtgctcg ggacgcgcgc cggcgtttcg ttcatcccgc
     5701 cacatctctt gcaggaggtt gtggaggttt cggaggacac tcgtcagcgt gacgtcttcg
     5761 ggaagcagcg cctggccgag ggaacctaca cgtccggcga aatcgacgtc tcggtctggg
     5821 cggatcatat tgaggcggac taccaggagt gggcgaaggc gaacgggagt ggagcatgag
     5881 cacgcgacac atcgagccgg cgattccccc agggtgggaa cggttccgcg gcgaagacgg
     5941 ggtgaccatt cgcgatgttc gtgtcattct caccgcgccg gacaacatcc gtctggttgt
     6001 ggtgaaagtc gagacgtcgg agccgggcct ctacgggctg ggttgcgcga cgttcacgca
     6061 gcgcccattg gccgtgaaga cggccatcga ggagtacctg cggcccttcc tgatcggccg
     6121 cgacgttcac gacatcgagg atatctggca agcctcgttc gtgagttcct actggcgaag
     6181 tgggcccgtg ctcaacaatg cgatgtcggg tatcgacatg gcgctttggg atatcaaggg
     6241 gaaggtcgca aatctgccgg tctaccagct tctcggcggc aaggtgcggg aggcggcgga
     6301 tgtctacgtc cacgcctccg gttcggagcc ggaggaggtg gtcgagcgct gccaggagtt
     6361 actcgaagag ggctatcgcc acctgcgttg ccaggtggcg gtcacgggct acgcgaccta
     6421 cggcgtctat tcgaagaagc aggaccgaac ctatgcggtg cggatcgaca cccatcagga
     6481 ccggtggtcg cccgaagcgt acctgcgtcg cgtgccggcg atgttcgagc aggtgcgaga
     6541 cgcactcggc tccgacgtcg agctgctgca cgacatccac gagcgcatac cgccgatgca
     6601 tgccatccgg ctggcacagg aactcgacca gtaccggctc ttctttctcg aggacccgtt
     6661 ccctccggag gacaacggct ggttcccgat gctgaggcaa cacagtgtta cgccgatcgc
     6721 gatgggggag ctctacgtca accaggcgga gtacctgccg ctgatcgagg gacgactcat
     6781 cgacttcatc cgcgtgcacc tctcggacat cggtggcttg acaccggcac gcaagctggc
     6841 ggcgttgtgt gagttctatg gcgtgcgcac ggcctggcac gggccgggtg atgtctcgcc
     6901 ggtcgggcat gcggccaacc tcgcgcttaa tctgagcagc agcaatttcg gcattcagga
     6961 ggcgacgttc tttaccgagc gggcgcggga ggtttttcca ggcacgccgg agctgcgcga
     7021 cggcgcgttg tggagcaacg accggctagg ccttggggtg gacgtcgacg aggagctggc
     7081 ggcgaaatac ccgttcccgg atcatcccgt gaacggtgcg tggcccgaga tccgccgggt
     7141 cgatggcacg gtgcagaagc cgtgatgttt cgggaccgct gaagatacat atcccttgag
     7201 cacccaaacc gccttccccc tcaccggact ggctaaaatt cgtggtccat gctggcgtca
     7261 atctcctgaa gacgaccttc ggcgatccgc atcactcggg tggcgatctg gcggatggcg
     7321 tatcggtcat gcgagatgag caccacggtg cccgggaacg cctgcacggc cgcctcgaac
     7381 gcggcgcgcg cgtccacgtc gaggtgattg tgcggctcgt caaggagcag cagatccgct
     7441 ccggggatcg cgatcaacgc cagcatcaac cggctgcgtt ccccaaagga gaggtcggcc
     7501 actcgacggt ggacggcgtc accgccaaac aggtagcggt ggagctccgt ccgcagcgca
     7561 gcttcggatg cggccaagca cttctgcaca ctctccaaca cggtaagcgc cgggtccagc
     7621 gtgtcctggt cctgcgcgag cgtgccgata ctcacgccgg gagccaggcg ccgatagccc
     7681 gtgtccggct caagctcgcc agagatgacc cgcagcattg tcgtcttgcc agcaccgttc
     7741 tcgccaacga cggcgagccg gtcaccgtag cggagcgtca gtgtgacgtt gtcgaggatg
     7801 cgatgcccac cgcgttgcag catgacctca tcgagcgtga cgacatcccg ggcccggaca
     7861 tccggttcgg ggaactggag cgcgacgccc cactgcggct tcggcttttc ggcacgctct
     7921 tccgactcca gcatgcgctc gagccgtgcg cgtcgaacgg tagcagcccg ggcgaccttc
     7981 gcggcccgct tgcggtagtg gaagtggatt gtttcgcttt ccaccttgcg cgacgaccgt
     8041 tcctgctggt cgatgctggc ttggagcccc gcgatcttct cccgctggcg cttccacgtc
     8101 tcgaattccg tcgctgcacg cctccggcgc gtctccaccc agtcctcgta accgccagtg
     8161 tggagctcaa cggtgctgga accggtttcg aacgcggcga tgtgagtcac ggtctcttcg
     8221 aggaaggcgc ggtcatgcga gacgatcacg atagcgccgg ggtagtcagc gatgaacttc
     8281 gcgagccatc gctgcgccgc gacatcgagg tggttggtgg gctcgtcgag caacaggaga
     8341 tcaggacgca ccgcgagcat ggttgccagc gcagcgcgcg ttcgctctcc accagagagc
     8401 tgcgacagcg ggcgctccgg tgcgtcggga ggcaggtcga agcgcgcgaa gtgggcctcc
     8461 agacgatcgg cgatgtcgta gccacctgaa gcctcgaagg ccgcttgtgc ctgttcccag
     8521 cgatcgagca cgatctcgct tgcgccatac ggttcagaca gcgcttcggt agcgagatca
     8581 agctcacgcc tcgtgccgaa ataaccacca gaggcggtgt cgagcgcgtc ggcgagcgtg
     8641 ccagtttcga gatcgagaat gccctgtgga agatagccga tacgggcgcc tggcgccaag
     8701 gtgcgtgtgc cggaggtggg cgcacacacg cctgccatga tgcgcagcaa cgtggatttg
     8761 cctgcaccgt tcggcccgat gataccgagc cggtcaccgc ggttgatggt gagcgtgagc
     8821 tggtcgaaga gaagcgtatc gcccgcgcga tacgaaacgg aagatagcga aaacatggac
     8881 gtcctgcccg tggtgaatcg cgaagccgcc catcgcctgg cgatgaagcg gatcgttggc
     8941 tgttcacccc acaggaggga cctgcggggc agggtcagtc cgtcatgtgt tcgctatcct
     9001 cgtcgagatg cacccgttcg cacccggggc ctagccggca gcttcgtctc cgccaagcgc
     9061 cgcagtggcc tcggcttcaa gcgccgtgag ggcgtagttg tcgatctcct gcgacaatgc
     9121 gtcgcggaga ttgatcagat caacgaggtc cagaccggcg accaccggcg tgttctcgtc
     9181 atcgctgtcg tcttcgcttg caggtacgac acgaaactcg acatcgactg tttcggcgtc
     9241 atcaccacca tctgccggaa tctcgtccat gtacagattg tacgtgtcca tcctgcaatc
     9301 tccgtgtgtg tccagctgct gccgaacata ccaatgtccg accgcttcgc tgcctccccg
     9361 gtctgcgagt acgctccgga tcccgatcgg atcgcgccgt cggtttccct agtgtacaag
     9421 atcaacagaa tctggggacc ggggtagccg gagttgctgc cagcgggtca cgccggaata
     9481 tccatcgcgt attatttgaa ctctgctgaa cgccctgtcg cgtctcgctg cgccggcctg
     9541 cccgggtgcg ccatgctcct cgagaggatg acggcaatgt cgtctgcacc ctttatgacg
     9601 aaaggaataa cacgacgcgc cataatggca gccggtgcgg gactcgcgct cggcggctgc
     9661 tcacccctcg acggtgagga ggacgtcgcc gaggaagtgg tgacggcccc cgctgagctc
     9721 gtcgatgacg tggcgttggc gacgcaggcg ccgccaacgc ccacgcaggc tcccgtcgcc
     9781 tcggcggtcc ccgggtatct cgatccggaa cggtggtacg cccgctcgct cgtggtcgcc
     9841 acagcgggag tcggcgacta tctcgaggcg ctcaccgctg ccttcttcga gcccttctcg
     9901 gaggcgaccg gcgtcactgt gcaacaccag gcgttcgggc gagacggcat cgccaacctc
     9961 gtcgatcagg tcgaaaacgg tgaggtcgtg tgggatgtcg tgctgattcc caccggagac
    10021 gtactgccgc tggcgcagca cggctatctc gagccgatcg actacgcgat cgtggactcg
    10081 acctcgctct atgagccagc cctgatgcag cacggtgtcg gcgcgatgta ctactccacc
    10141 gtcatgacaa gctcggcggc actggaggat gagccggatt cctgggacga cttctgggat
    10201 ctcatgacct tcgacggcac gcgtgcactc aggcgctatc ccgtcggcac gctcgaattc
    10261 gcgctcctgg cagccggcgt accgcaggac gagctctatc ccctcgacgt gccactcgcg
    10321 ttcgagatgc tcgatctcat tcgcgaggcg acgatcttct acgaggacaa caaacagcct
    10381 gtggagctgg tgcgcaccgg gcaggtgggg ctggcgagcg cctggaacat ccgggcggtg
    10441 ctgccggacg tgacgtcgct cgtgaatctg cattggcagg gcgggatgat ctcggccgac
    10501 agttgggcag tgccccgcgg caccgagaat ctcgacgtgg caatgggctt catcgatttc
    10561 gcaacacggg cggtaccgag cgccaacttc agcctctacc tgccgttcgg gccggcaaac
    10621 cgcgatgccc ttgagctgat ccggcccgat gtggtcgagg tcctgccgac cgggcctgcg
    10681 cacttggagg ttcagttctt cgagaactgg gcctattggg ccgacaaccg ggacgagctc
    10741 gttgcgcagt tcgaggactg gctgctcaac ccgaccgcca gcccgacgga gggctagtat
    10801 ccctcaaccg agccctcggc ccgtggatcg gcggcaccga cgagctcgcc ggtctccggc
    10861 tgtcgaagca ccatctgggc atagccggcg tgcggccatc ctggctcgac ctgtgccacc
    10921 tggtgaccac gctcttgcag ccccgcgatc acgtgccatt ccatgcccgc ctccacgacg
    10981 acggtgaagg ggtccaggcg cgcgtcaccg gagacccatc gtggtcgagc gatcgcctcg
    11041 cccggtgtgg ctccgctgtc caccaaatgg ctcaccaact gcatgtgtac ctgcgcctga
    11101 gcgtcgcctc cctgggtgcc gaatggacca cgaaatcgcc cggggatctc gatcatgcct
    11161 ggcatgagcg tgtggagggt acgctttccg ccagccaact cattcgcatg accgggcacg
    11221 agcgagaagt aggagccgcg attctggagc aggacgccgg tctcgccggc gataaccgct
    11281 gatccaaagc cggagtagat gctctggatc agcgaaacca tgagaccggt ctcatcgacg
    11341 acggcgaggt agatggtatc cccaggcaag gtgacttggc ctgttgtcgc catcgctggg
    11401 gtgaactcgg cccagagttg gtctatcgtg ccggcatcga ggtaggcctg gacatcgacc
    11461 gtcattgctg ccgggtcgga catgtagcgg tcgcgaatgg agaaggcgcg ctttttggcc
    11521 tcgatttcca cgtggagcca agcgggatcg tcccaagtgc cgggaaaggg cattcgctcc
    11581 atcatctcga gaccaatctg tgcggtgacg ccttgtgagt tcggcgggag ctcccaaacc
    11641 gtggcgtctc gatagcgcga ctccaccggt gtgacccact cgcctcggtg cgttgcgagg
    11701 tcttccacgg tcatccagcc gtcgccaccg cctacagcct ccacgatcgc ggccgcgacc
    11761 tccccacggt agaaggcgga gcgtccctcg cgcgcgatgc gctccagcgt gtcggccagt
    11821 ccaccattca ccagccagaa tccgggctga ggggcaaagc caccgtcgag gtagattgca
    11881 gcggtcagcg gtgattgcgc caagtcgttc acttctgcag cgatcgccgc cgacaggcgc
    11941 ggtgagatct cgaagccgtg gcgggccagc tcgatggcag gctcgagcaa ccgttccatg
    12001 ggcagggaac catagcgccg gtgtgcctgg tgccatgcat cgatcgtgcc cggcacggtg
    12061 accgtccacg gtccgcgccg aggcatgcct gagaagccgg cgttgcgcac cacgtcggcg
    12121 ttcgcggcgg cagcggaccg gccggagcca ttgaagccga gcggcccctc ttcacctggt
    12181 tcccagattt ggaagaagca gtcgccgcca atggaggtct ggtcggggag cagtacggtc
    12241 agtgcggcgt tggcggcgat agcggcatcc gccgccgttc cgccctcgag cagaacgtcg
    12301 accccgatgg tcgatgcgac gtggtggggc gtggcgatca tggccgtggg agccgtcgtt
    12361 gatggcatga ggtgatgctc cggttggggc ggggacgccc ggcgaggatg acgcgaatag
    12421 ggtaagatgg tagccgagtt cggaacccgg ctcaacacgt ctgtgcaaca ggagattcga
    12481 tgcatccacg cagaatactg cgaggcaccg ttctggtgtt gctcgcatca ctcaccatgt
    12541 ttgcagtggc agggggcagt catgcacagg tcgctgatga cacccgtgcg gctctggcgg
    12601 cgatcgcgtt gactgccgcc gaaatgccct ctggctatga gctcgtcggc gaagctttcg
    12661 tcggacctga tgggggcccg attgcgggcg aagtgcccgc cgtgctcacc gacgccggtt
    12721 ttctcggcgc atacatgagc gtttacgaga atgcagatgg cacggtgaca atcacgagct
    12781 atgcatcggc gtggacggat gccgatgcag ccgaaaccgc gttcgcgctt ctcgagaccg
    12841 aggatgcagg cgagaccgat gagtctctgg atgcgggcga cggcccggcc gagttgaccg
    12901 tcgtgggcga gacgatggat gcgaccttcg tcgtcgaccg attcgtggtc ggcgttgccg
    12961 ccacggggag tgacatcgat cgggatgacg tcatggcact agtgacgctg ctagagtcgc
    13021 gggcgaacgc cgttgccgac ggccagtttc cggcggggac ggatgctgca ctgccggcat
    13081 ccgtgctcga taccagtgcc ctcggcaccc aggtgcgggc cgggtatctt tcggcgactg
    13141 aatccgagca gctctacggg ctgaccgggt cagcgctcgg aggcatgcag gcatcgtggg
    13201 tttcgctggt caccgttggc gatgggggcg agccaccgta cgtcgccatc gcggcctcaa
    13261 acttcgagtc ggccgacaac gccgcgcgcg tggtcgacca ggccgaagac ttggtaccgc
    13321 tgaccgtcga tctgcagccc gtcgagggga tcacggtcga agacgccgac actgcgcggg
    13381 gcttccagta cagcagcacg atgagcggta ccaccgacac gccagacagt gtccgggttg
    13441 tcgctcaggt tggcgatcag gtcgtggtgg tcgacgtgca gggagccacg agcgccgagg
    13501 aggttcaagc tgcggcggtc tcgctggcaa acgcgcaggt ggcctgcatg atggaagaag
    13561 ggaactgcga gcttccagag gttaccctcg gcggagcgta gcgttcttca gccacagcgt
    13621 agcgttcttc agccacgttg agacggccgc cgggcgggtt cgcaaaggaa ctagcccatt
    13681 actgcgcagg atggccgcaa ctgccctcgc ctcaagcccg ccgatcatcc cgctaggagc
    13741 ggtgagcgtt cgaagtggag caggagttct gagcgcctca gggtgtggct gttgccgtca
    13801 gtggaggagg ggtgccgggc tccaggacgc gtgccgaacg tgtcgcctgg aggtcgctga
    13861 ccacggaggt ccgccgggca tcctgctgct ggcctcgctc ggcatcctgt gaggtgccct
    13921 cggagcaacc gacgagcacg ccgagcgcca tggcgacacc ggtgaccaac gccaccagcc
    13981 gcagatgccc atccctcctg tcagtcttgc ccggtccggg gcggccggta gtcgccgaag
    14041 gcttcctgat actcatctgc aacctcgacc gccgacttgt ggacgtagat gcgggtggtc
    14101 tgcggtgacg cgtgcccgag gatcgtctgt acggtcgact cacgaacccg gcggcggacg
    14161 agctcggtcg cgagcccgtg ccgaaaggag tgcggggtga ccacgctctc gatgccgacc
    14221 tcgttgcaga tctccgacac aatgtgctcg accgtccgcg ggctaatctg cttgccggcc
    14281 actccgacct tgtcgcgccc gctgaacgcg gggttggcgc cggcgccacg cggctggtcg
    14341 ccccgcgcct tccagtaggc gctcagggcg tgggccgtat cagcgtcgaa gtgtaccgtg
    14401 cggctcttgc cacccttggc tcggtaaata tgggcggcgc catcgtcgag tgagatgtcg
    14461 cgccgccgca tggcgcacag ctccgagacg cggacacccg tccgatacat gaacaggatc
    14521 agcgcctgca ccttgagacg ccgcagctcg aggtgcggct tttcctcacg tggccggctg
    14581 ctgacgtgcg cgacgatcgc ctcgaggtct tccaggctca catcgggcgg cggcggggtg
    14641 aagcgcggca tcatcgcagc gacccggacc cggatgcggt cagtgctgag gtcggggtgg
    14701 agatcgtatg cgctgagata ggagcacagc ttgcgcacgg ctgccagcgt gttctgtgcc
    14761 gtccgcatcg ccgacacctc ttccggcgtg cggatgtcgt ccggcaccag cactgacgcg
    14821 aaattcgtga cgtgctcgac ggtaagtgcc tcgaccggcg ccgtgaccgg gtcaatacct
    14881 tcatggcgcg cgaggtgtcg caagaatttt tcgataccga tgcggtaggt ggtccggctg
    14941 cgcggcgcga gccggaggtc gccgaagaag atgtcgacgg cggcggcgat ggtcagattg
    15001 tcagtcccgg tgctgggtgt catgtgcccc tcctttgatg tacgtacatt atacgcaaga
    15061 ggggatcgcg cgccaaagtg acgcggattt gcgggaagcg aggcttgccc tctgcgcctc
    15121 agaccgtcga tgcaatgtac tccatgggca atgtcctacc tccgctgcgc gtagcgctct
    15181 accagatcgg gcaactgttg actgaaggcg ccattggaga taacgcgcgt tactctagcc
    15241 ttgcgggcca tccggaatgc ctcggtatcg gtgtgggcac cgaacgcga
//
LOCUS       DASYWL010000085         5228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_379030, whole genome shotgun sequence.
ACCESSION   DASYWL010000085 DASYWL010000000
VERSION     DASYWL010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379030"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="VGR22_03225"
     CDS             complement(<1..868)
                     /locus_tag="VGR22_03225"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HEV2127612.1"
                     /translation="MAITERGAPRRTIGSGLDVFSDTPLPPWARIRQELDSTDVGSIP
                     DAVAREFAKPGIGDQITKGKVVVLTAGSRGIDRIAEVLKACVIEIRNRGGEPFIIPAM
                     GSHGGATAEGQMELIAHYGVTEAAMGAPIRASMETVHLGTLDGGVPVWFDRIAHETAD
                     LVIPVGRVKPHTDFRGPVESGLMKMIAIGLGKQHGASWFHSQGIHTFGDLIPKVAEFT
                     LQQEPIPFGIALVENGHCKLAMIEAVLAARILEREIELLEIANGTLATLPPVERLDVL
                     IIDEIGRLMSDAV"
     gene            complement(953..2050)
                     /locus_tag="VGR22_03230"
     CDS             complement(953..2050)
                     /locus_tag="VGR22_03230"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEV2127613.1"
                     /translation="MASATNWARFRALMVLLVAVLLVPLPAPVHGAQDDRTDSVTAAA
                     IELSRLERSGDLLTLHDRLHPDVRMVVSRAALAAWYAGPDVVIPTDDPEILSVEFGSW
                     TWPVTERAYADVATVVLRQQGTKQGAVVEQIELQHFLFDGVRWRWFFGADMPFIEALE
                     AQTTPDAGPSVLGDIAYARVDLVWSEIYSQADIPYGSPDSINPIPELPVGTGCGQMTI
                     DEYAEVFYCNLDQEIYYLIGFQEQMARQFGTYAWPHIIAHEWGHHIQFLEGIRSTDAP
                     ELDDGVYVIEQELQADCLAGVFAQEAVARGWLTMEDLNDAYQVSIFAGDLPGTTFDDP
                     VAHGSAEQRQQAFTMGLEDGLVGCNLDLVSD"
     gene            2269..3249
                     /locus_tag="VGR22_03235"
     CDS             2269..3249
                     /locus_tag="VGR22_03235"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEV2127614.1"
                     /translation="MFHTDLLSVRTRRAVLAAMLTVAMLLGPLAALTAAQDDEPLQVV
                     ASFSALADIVRQVGGDHIEVTSLVPAGGDVHTFDASPDDIRTLEDADLVVQVGDDYEP
                     WLDDLLETAGDDVPRYEAFAAIDHNLSEHGDEHEGDDHAGDSHDRGDEVHRWLDVQAV
                     IHTVTHLGETLAEIDPDNADAYTANAETYIAELEELDAWIVEQTATIPEDQRELITVH
                     HSMGAFADAYGYEIVGVLLESHSTEGADASAGHVAELVEIVEEREIPVLFVDTPGGED
                     LLQPVASEADVEIAPPLYVETLSADGEGPQSYIEMMRYNVETIVNALSRS"
     gene            3460..4089
                     /locus_tag="VGR22_03240"
     CDS             3460..4089
                     /locus_tag="VGR22_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127615.1"
                     /translation="MSSENDHPRATAAFFDDGLWDIALGLGILTFALGSVIDAAYLPI
                     LAIPVLAVITWIGKRRIAALRPSPPVMPKRVESHRTSLWTGGGTVVVLGYLIPILSMA
                     GHNGDSLIWDIRRIPVGLALAAGVAIIGPLSWWWYLYAGVIASVTMIAWLLEWPAWAE
                     FGVSGNAILAAGAFILRRFATSGETADTTSPRNPHAPEPRPATNQEEFP"
     gene            4086..>5228
                     /gene="aroA"
                     /locus_tag="VGR22_03245"
     CDS             4086..>5228
                     /gene="aroA"
                     /locus_tag="VGR22_03245"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEV2127616.1"
                     /translation="MTSVPSPTTWPDAIEIQPARDPVSGTPEIPGSKSITNRVLVTAA
                     LATGTSRLTGALFSDDTYYMTRALNQLGITVRIDEDSRTMEIEGAGGLIPASSADLFI
                     GNSGTTARFLAAVVALGTGRYRIDGDEAMRKRPIGPLLDALRQLEVDATSDNGNDCPP
                     ITISSTGLPGGEATMPGNLSSQYLTGLLLAAPAARGDITIHVEGELISRPYITITAEV
                     MDAFGVEVDLGETSFRVPGGQRYESTEYAIEPDASAASYFFAIAAITGGTITIEGLGP
                     SALQGDLEFVRVLEAMGCTVEMTATATIVTGPSGGRLRGGEFDMGDISDTSQTLAGIA
                     PFADGPVTFRGVAHNRVKETDRVGNVVRELRKLGQEVEEFEDGMAIH"
BASE COUNT          925 a         1781 c         1612 g          910 t
ORIGIN      
        1 tgaccgcatc gctcatgaga cggccgatct cgtcgatgat cagcacgtcg agccgctcga
       61 ccggtggcag ggtcgccagc gtcccgttgg cgatctccag cagctcgatc tcccgctcga
      121 ggatccgcgc cgccaggacc gcctcgatca tcgccagctt gcagtgaccg ttctccacca
      181 gcgcaatgcc gaagggaatc ggctcctgtt gcagcgtgaa ttcggccacc ttcgggatga
      241 ggtcgccgaa cgtgtggata ccctggctgt ggaaccagct cgcgccgtgc tgctttccga
      301 gcccgatcgc gatcatcttc atgaggccgg actcgactgg accgcggaag tcggtatgcg
      361 gcttcacccg gccgaccggg atgacgaggt cggccgtctc atgagcgatg cggtcaaacc
      421 agaccggcac accaccgtcg agcgtgccga gatggaccgt ctccatgctc gcgcggatcg
      481 gcgcccccat cgccgcctcg gtgacaccgt agtgtgcgat gagctccatc tgtccctcgg
      541 cggtggcgcc gccgtgactg cccatcgccg ggatgatgaa cggctcaccc ccccgattgc
      601 ggatttcgat cacgcacgcc ttcagcacct cggcgatgcg atcgatacca cggctgcctg
      661 cggtcagcac gaccaccttg cccttggtga tctggtcacc gatgccgggc ttggcgaact
      721 cccgtgcaac ggcgtcgggg atgctgccga cgtcggtgga gtcgagctcc tgccgaatgc
      781 gggcccatgg tgggagcggg gtgtcggaaa agacgtcgag tccgctgccg atggttcgcc
      841 ggggtgcgcc gcgttcggtg atcgccatgc ctgcctcctg ggtcgggtat cgtctcgatg
      901 accccattgt atgcccgaat ccgaacctgc gagttcgata cctcctctgg atctaatccg
      961 agacgaggtc gaggttgcag ccgaccagac cgtcctcgag ccccatcgtg aacgcctgct
     1021 ggcgctgttc ggcgctgcca tgcgcgaccg gatcgtcgaa ggtcgtgccc ggcaagtccc
     1081 cggcgaagat gctgacctgg taggcatcgt tgagatcctc catcgtcaac cacccgcgcg
     1141 cgaccgcctc ctgggcgaag acgccggcga ggcaatccgc ctggagctcc tgctcgatga
     1201 catagacgcc atcgtccagc tcgggcgcgt ctgtcgagcg aatcccttcc agaaactgaa
     1261 tgtgatgccc ccactcgtgt gcaatgatgt gcggccacgc gtaggtgccg aactgtcgcg
     1321 ccatctgctc ctgaaacccg atcaggtagt aaatctcctg atcgaggttg cagtagaaga
     1381 cctctgcgta ctcgtcgatc gtcatctgcc cgcagccagt gccgacgggg agctcgggga
     1441 ttggattgat actgtcgggt gagccgtagg gaatatccgc ctggctgtag atctccgacc
     1501 agactaggtc gaccctcgcg taggcgatgt caccgagcac cgacgggccg gcatccggcg
     1561 tggtctgtgc ctcgagtgcc tctatgaacg gcatgtccgc gccgaagaac catcgccagc
     1621 gcacgccatc gaagaggaag tgctgcagct caatctgttc cacgactgcc ccctgcttcg
     1681 tcccttgctg gcgcaacacc accgtggcga catcggcata ggctcgctcc gtcaccggcc
     1741 aggtccacga accgaactcg accgagagaa tctcgggatc gtcggtcggt atcaccacgt
     1801 caggaccggc ataccaggcc gcaagggccg cccgcgagac gaccatgcgg acgtccgggt
     1861 ggagccggtc atgcaacgtg agcaaatcac cgctgcgctc caggcggctc agctcgatcg
     1921 ccgccgccgt caccgagtct gtccgatcgt cctgcgcgcc gtgcactggt gccggcaagg
     1981 ggaccagcaa caccgcgacc aacagaacca tcaatgcccg gaaccgggcc caattcgtcg
     2041 ccgatgccat gccaaacgcg ctcctcatga ccgaaggaac tccaccgtcg taaggatacg
     2101 tcctcgccca tcccaaaccg tgcattctcg gcaccggcaa acattgtcag ttcacaatgc
     2161 gcctgtctat actgagacat ggtatcacat ccccttggtg tgacagggat atagatgcgt
     2221 aacgttctgc tgtttgtccc cattgaaccg aggaagagga acccgcccat gttccatacc
     2281 gatttgctat ccgtccggac tcggcgcgct gtgctcgccg cgatgctcac cgtcgccatg
     2341 ctgctcggcc cgctggcagc cctgaccgcc gcacaggacg acgagccgct gcaggtggtc
     2401 gcctcgttca gcgcgctcgc cgatatcgtg cggcaggtcg gcggcgacca catcgaagtc
     2461 acctcgctgg ttccggccgg tggcgacgtg cataccttcg acgcgtcgcc cgatgacatc
     2521 cgcaccctgg aggacgccga cctcgtggtg caggtcgggg acgactacga gccgtggctg
     2581 gacgacctgc tggagacggc aggtgacgac gtcccgcgat acgaggcctt cgcggcgatc
     2641 gaccacaacc tcagcgagca cggcgatgag cacgaggggg acgatcatgc cggtgacagc
     2701 catgaccgcg gcgacgaggt ccaccgctgg ctcgacgtgc aggccgtgat ccacaccgtg
     2761 acacacctcg gcgaaaccct ggccgagatc gacccggaca acgccgacgc ctacaccgcc
     2821 aacgcggaga cctacatcgc cgagcttgag gagttggacg cgtggatcgt cgagcaaacg
     2881 gcgaccatcc cggaggacca gcgcgagcta attacggtcc accacagcat gggtgcgttt
     2941 gccgatgcct acggctacga aatcgtcggt gtgctgctgg agtcgcactc caccgagggg
     3001 gcggacgcct cggcgggaca cgtcgccgag ctggtcgaga tcgtcgagga gcgagagatc
     3061 ccggtcctgt tcgtcgacac gcccggcggc gaggacctgc tgcagccggt cgcgtctgag
     3121 gcggacgtcg agatcgcccc gccgctctat gtcgagacgc tgagcgccga tggcgaggga
     3181 ccgcagagct acatcgaaat gatgcgctac aacgtcgaga ccatcgtcaa cgcgctcagc
     3241 cgcagctgag accatccccc tccccacgct cacgccggcc acgccgacga cccgcgtccc
     3301 tgccgccatg gggtggagcg atatggcgag ttcagtgtag gtttgcacag caggtgtcgg
     3361 gcgtcgtcct ctccctgatg atgatgcccg tctgatttcc tccagaacct gaggcgcgca
     3421 tctgtaccct cgattgtgcc ccttgaggag cgactggcca tgtcatcgga gaacgatcat
     3481 ccaagggcaa ctgccgcgtt ctttgatgac ggtctctggg acatcgcact cgggctcggc
     3541 atcctcacct tcgcgctcgg gtcggtgatt gatgcggcct atctaccaat tctcgcgatt
     3601 cccgtactcg ctgtgatcac ctggattgga aagcgtcgta ttgcggcgct gcgtccttcg
     3661 ccgcctgtaa tgcccaaacg agtcgagagc caccggacat cgctctggac cggcggcggt
     3721 accgtggtcg tgctcggcta cctgatcccg atcctgtcga tggccggaca caacggtgac
     3781 tccttgatct gggacatccg gcgcataccg gtcggattgg cccttgcagc cggcgttgcg
     3841 ataatcggcc ccctgagctg gtggtggtac ctctatgccg gggtgatcgc cagtgtgacg
     3901 atgattgcct ggctactcga gtggccggca tgggcggagt tcggagtttc cggaaacgcc
     3961 atcctcgcgg ctggtgcctt cattcttcgt cgcttcgcta ccagtggtga aacagctgac
     4021 acgacctcac cccggaatcc ccatgcaccc gaaccgcgtc cagcaaccaa ccaagaggag
     4081 ttcccgtgac cagcgttccc tctccaacca cctggcccga cgccatcgag atccagccgg
     4141 ctcgcgatcc cgtctccggc accccagaga tccccggctc caagagcatc accaaccgcg
     4201 tgctggtcac cgccgcgctt gccaccggca cctccaggct caccggggcg ctcttcagcg
     4261 acgataccta ctacatgacg cgagcgctca accagctcgg catcaccgtc cgcatcgacg
     4321 aggactcccg cacgatggag atcgaggggg ccggcgggct catcccggcc agctccgccg
     4381 atctcttcat cggcaactcc ggcaccaccg cccgcttcct cgcggcagtc gtcgcgctcg
     4441 gcacgggtcg ctatcgcatt gacggcgacg aggccatgcg caagcggccg atcggccccc
     4501 tcctcgacgc cctccgccag cttgaggtcg atgccaccag cgacaacggc aacgactgcc
     4561 cgccgatcac gatctcctcc accggcctgc ccggcggcga agcgacgatg cccggcaacc
     4621 tcagcagcca gtacttgacc ggactgctgc tcgccgcacc ggccgctcgc ggcgacatca
     4681 ccatccacgt cgaaggcgag ttgatctcgc gtccctacat caccatcact gcggaggtga
     4741 tggacgcatt cggcgtcgag gttgatctcg gtgagaccag ctttcgggtc ccgggcggtc
     4801 agcgatacga gtcaacggag tatgcaatcg agccggacgc ctccgcggcc tcctacttct
     4861 tcgccatcgc ggcgatcacc ggcgggacga ttaccatcga ggggctgggc ccctcggcgc
     4921 tgcaggggga cctcgagttc gtccgggtgc tggaggcgat gggctgcacg gtcgagatga
     4981 ccgcgacggc gacgatcgtc accggcccgt cgggcggtcg cctgcgcggc ggcgagttcg
     5041 atatgggaga catctcggat acatcacaga cactggcggg catcgcgccg ttcgccgatg
     5101 gtccggtgac cttccgtggc gtcgcccaca atcgcgtgaa agagacggac cgggtcggga
     5161 acgtggtcag ggagctgcgc aagctcgggc aggaggtcga ggagttcgag gacggcatgg
     5221 cgatccac
//
LOCUS       DASYWL010000086        15762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_153678, whole genome shotgun sequence.
ACCESSION   DASYWL010000086 DASYWL010000000
VERSION     DASYWL010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 15762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15762
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153678"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VGR22_03250"
     CDS             <1..230
                     /locus_tag="VGR22_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127617.1"
                     /translation="SPIPGGAAVREGLMYVVARLSGYSPEADAILAAALIYRFALFAA
                     IPILFGLNRLWLNSSGNPGINGREVPADERI"
     gene            236..871
                     /locus_tag="VGR22_03255"
     CDS             236..871
                     /locus_tag="VGR22_03255"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV2127618.1"
                     /translation="MSISETYLYKPPQAVETSNFAKHTSDNPLQRRLIDRFHAKLTEL
                     VKAEDPETLLDAGCGEGFTDEIFLQAMPALQLTGFDPHEPAIELACMRNPRAEFLVAD
                     IYNMPFEDDSFDVVCCFEVLEHLHDAPQALSKLARVARKAAVLSVPHEPFFCLANMAR
                     GKNLDIDPKGSDPDHRNFWSREAFGAFVQREMDVAHLTGSFPWSICVARPR"
     gene            947..2608
                     /locus_tag="VGR22_03260"
     CDS             947..2608
                     /locus_tag="VGR22_03260"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEV2127619.1"
                     /translation="MPDTPFQHPPSTTPLGGSSRRHERRSNFARNWTGYVPMVRVCPA
                     CDTENDQHSRFCVGCAANIDDVEPTPSRNPNSGVRAMEARIRREADVARRYRPFTAAG
                     GTGFIITGVMLLLAATMFPLPFWIRLGTWLAGLAFTAAGLWRMRFDGDAIRRTGFVLA
                     ASTIGIVALVLTRGTLPADPAALLPTPQPTQVAMQATPPPASPVAGATLTGSVTTLLG
                     GPSHSGIQDGPPPSGNPALAWRFDTGKEILSQPVIADGTVFITNRAGFLYAIDPLTGQ
                     QRWRVELGDYALRTTPTWHEGRLYLVSGFEAIALDAQTGETVWETTIRYAGTASPTVA
                     HGNLYVVSQEGWLYALSLENGGEVWKAPTDGISFGPPSATEQYVVIGTDNGKVTAFTP
                     EAGRQAWRRDFESAIYSPPVIVDGTVWIVTDDGRLLGLGLDDGGDRFALEATADLPVT
                     ARDATVYVPSTDGGLYAMDAESDAVLWFASAGGAVRSGPVQSGGQVVISGGNRIAGLD
                     RESGEQIWYYLAGDSIVAPPAIVDGHVFFGARDGVLYAVTDRGSP"
     gene            complement(2817..4136)
                     /gene="hemL"
                     /locus_tag="VGR22_03265"
     CDS             complement(2817..4136)
                     /gene="hemL"
                     /locus_tag="VGR22_03265"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEV2127620.1"
                     /translation="MPAESTTRTTPHYAKSAEAYATAQQVMPGGVNSPVRAFRAVGGT
                     PIFVESGSGSRFTDIDGNTYIDYVLSWGPLILGHAHPKVVEATSNALRKGTSFGAPTV
                     AETRLAELIVERVPSVDMVRLVSSGTEATMSALRLARAATGRDKIVKFAGCYHGHGDM
                     LLVQAGSGVATLGLPDSPGVPKGATQDTLVATYNDLDSVRALFHQYGDELATVIVEPV
                     AGNMGMVEPVEGFLEGLREITAKHGALLLFDEVMTGFRVAPGGAVERFGIEPDIVTFG
                     KVVGGGFPLAAYAGKREIMEHVAPAGSMYQAGTLSGNPVATAAGIATLEVLAEEGVWA
                     TAADRAEQLVHGLRSAGVEVGVPVQAPAIGTMAGVFFKDAPVRNYAESAVDDGGRYAK
                     FFQGLLTRGVYFAPSRFEALFLSSAHSERDIDLAVEAAAEIMKDMAA"
     gene            complement(4189..5448)
                     /gene="hemA"
                     /locus_tag="VGR22_03270"
     CDS             complement(4189..5448)
                     /gene="hemA"
                     /locus_tag="VGR22_03270"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEV2127621.1"
                     /translation="MSKPSIIIVGSNHECASVDLRERLAFNGETLTGGLRALRQHVDE
                     ALIISTCNRTELYAVAEDGEQGRERIIDFLSSYHGMPRHVLDSMSYAHTDASAVRHIF
                     RVASGLDSIVLGEPQILAQIRDALEAARTAESAGAYLQRLATDALKVGKQARTETDIA
                     RNNLSISHAAVALAERERPDLATQRVTLLGAGKMASIAARVLAARGVAQLTIVNRSVG
                     KAEQLAAQIGATARPTTELREAIAESDVVIAAILTEEPVIVPLMLEGRTKPLLLIDLG
                     VPRIVDRGCGYVAQVEARDVDDLESVAHESRQRYSNEMNKVEQLVDRAIDDYLQWARS
                     RTASDAIAALRHQSDAVRDREVERALRRLGHLSERDQNVVRAMAKALTNTLMHEPVQS
                     LRNAGSELEIRSILAHLGVDPDEPDDR"
     gene            5715..7088
                     /locus_tag="VGR22_03275"
     CDS             5715..7088
                     /locus_tag="VGR22_03275"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEV2127622.1"
                     /translation="MPVRTIHEDYHEEFAASKALWERSSESIVGGITHDGRFIKPFPP
                     YVARADGGHKTSVEGNDLIDFCVGHGSVIFGHNDPDLTAAIQRQVPIGTHFSAGHEGE
                     VRWAEQIVKLVPSAEKVRFTASGTEATLLAMRVARGYTQKKTILKFEGHFHGWQDYAL
                     KGEKPPFENVSVPGVPSETLGTVSVVPSNDLAMLEERLVQGDVAGVILEPSGGSYTTI
                     PLADGFLQGVRDLTAKHDAVLIFDEVITGFRWSPGGAQQRFGITPDLTTMAKIVAGGM
                     PGGAVAGRRDIMDVIRFTDDAAWNSKRRVPQAGTYNANPLAAAAGYACLVKIDDQSVH
                     DHCDQIAADIRSGMNRVLEKHDAPGFAWGESSVFHVALDKRCTNRSGDDLRTPEGVDP
                     EYLKKSGGSPLGMLLEVGMLLEGVHLFHAGGLTCTKHDTTDVERTVAAFDQVVGRIKD
                     EGHFDGS"
     gene            complement(7114..7713)
                     /locus_tag="VGR22_03280"
     CDS             complement(7114..7713)
                     /locus_tag="VGR22_03280"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEV2127623.1"
                     /translation="MSNDRVFALVLAAGLSRRLGRPKQLLQLGGKPLVCHVVEAALAA
                     PIDGVVVVIGSHASEVEQALLDYQTYRVFNPHFSKGQGNSLSAGVRAIPSTVDAIVVL
                     LADMPGIRTEAIAAVVERWRQTRAPAVMARYTDGDGHPVLFDHSAFTDLARLQGDSAG
                     REVLRRLGDQVERVDVPLPGPPLDVDTEEDWERLQASWG"
     gene            complement(7706..8485)
                     /locus_tag="VGR22_03285"
     CDS             complement(7706..8485)
                     /locus_tag="VGR22_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HEV2127624.1"
                     /translation="MAAPPDSGAVYDEARRLAAEGTTAVVATVVEGDLVGSKALITSG
                     EITMGSLGSEALDESALRAVSTSAGTPASGLLEVDEGTRIFLDVFEPSRQLIIFGAVH
                     IAQALDTFARRLGYRVVVVDARRALATRERFPDADQLLVAWPADAYEQLTVTPNTAIA
                     ILSHDPKFDEPALLGALETGARYIGAVGSSKTNADRRQRLLDAGVSEEQIARVRGPIG
                     LDLGGSTPEEMAVSILAEMIAVTHGRDGRPLTEIKGAIRGE"
     gene            complement(8488..8802)
                     /locus_tag="VGR22_03290"
     CDS             complement(8488..8802)
                     /locus_tag="VGR22_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEV2127625.1"
                     /translation="MAEWLPEIDQWRSEGKKVALATVVNVIGSAPRPVGARMAINEKG
                     KMAGSVSGGCVETSVVLEAQQVLKHNTPRLAEYGISDDMAWDVGLSCGGTIEVFIEPM
                     DA"
     gene            complement(8795..10168)
                     /locus_tag="VGR22_03295"
     CDS             complement(8795..10168)
                     /locus_tag="VGR22_03295"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV2127626.1"
                     /translation="MQHDSQPLSQYSERHNQRSREPQVNAATIRRNLLRFARRLRAAG
                     MNVGPGQILEFVTALEQVSLERYDDAFHAARATLVTRPEQLSAFRAEFEQFWHDLLQE
                     RMMPLEEYLSSDEERTGKPKRGDTPEDRESRHGQSGEAQESDRRMLAIEGVDREGDIG
                     EREEYETPPEDVLLFSASEAFRKKDFSQFTDEEIAEARRIMAEMSWRLSTRETRRRQK
                     AVRGDFIDYRRTLRQSMKQGGVPIDLRFRTRKQRMRPLVLICDISGSMDRYSRMLLRF
                     VHALEHGLDSVEVFVFGTRLTRITRELRKRDVDQAIEDVVASVDDWSGGTRIGEAIKT
                     FNFEWSRRVLLSGATVVVISDGWDRGIPDLLGDEMARLQRSCRRLIWLNPLLGAAGYQ
                     PLTQGIRAALPYIDHFLPVHNLESLGALAALLGEADEQPPRRASHRVVSKRAAEREKR
                     EKGAIDG"
     gene            complement(10143..11081)
                     /locus_tag="VGR22_03300"
     CDS             complement(10143..11081)
                     /locus_tag="VGR22_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEV2127627.1"
                     /translation="METVNEVVQRRFGSVSELEEALREKKYVTDRGLATTLYLTLTMR
                     KPLLLEGEAGVGKTEIAKVLADLLDTPLIRLQCYEGLDANSAIYEWDYPRQMLFLRSL
                     EATGDLDREEVRKDLFSEEFLLKRPLLQAIDVAHDKAPVLLIDEVDRADQELEAFLLE
                     LLSDFQVTVPEVGTLRAEHVPIVILTSNRTREIHDALKRRCLYYWIDFPSFEKESRIL
                     TAKVPEASERLVTQICTFTQGLRDVDLFKSPGMAETLDWAAALEALGSRELSDEVVEE
                     TLGVILKYQEDVDRITGDVAQRLVAEALDATRQPTP"
     gene            complement(11217..11948)
                     /gene="tsaB"
                     /locus_tag="VGR22_03305"
     CDS             complement(11217..11948)
                     /gene="tsaB"
                     /locus_tag="VGR22_03305"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEV2127628.1"
                     /translation="MLTSYLGSYDRVTASASSEWVLAIDTSAERAGVALWNGTALHEM
                     VWPSDRRQTTEAMSRIDDLLASADVDVREIVGVVVAQGPGTFTGLRVGMSIAKGLAIA
                     LGIPIAGVPTLDAIALPWAQANRSVVAMLAAGRGRLVWQSISPGGPSAIPVNGTPAEL
                     LASLAGARPDAMVGELPVALREALSGAAVPVLSEPGLTSRIGAIALLGAHRLRHGHAD
                     DLVTLEPLYVHGLSKTSRPVRDARP"
     gene            12101..13054
                     /locus_tag="VGR22_03310"
     CDS             12101..13054
                     /locus_tag="VGR22_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV2127629.1"
                     /translation="MGDIGKHLVAPPRQQAYEVARPRRNWNILRKEWVLFILFAGPNL
                     FLFSLFSYWPMIENMRLSTQRWDLLSPVRINVGLDNYRYLIANDTFHQVLWNTLYFTV
                     AAVGLSLLLGLLIALLLNQRLRWRNGARAIVFAPYMLSGAAIGIVWAYIFDPRFGLLA
                     EMLGWVGLNSPDWLNRPEWAMPAIIIVYVWKNLGFTTVIFLAGLQSIPQDLYDAAKID
                     GANVLWRFRSVTLPMLSPIAFFLVVTSILSTFQSFDIISVMTDGGPVDATNTLIYYVY
                     EQGFVAFNAGRSAAAALVLFAMMMVITLIQLRYSESKVHYG"
     gene            13077..13919
                     /locus_tag="VGR22_03315"
     CDS             13077..13919
                     /locus_tag="VGR22_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV2127630.1"
                     /translation="MLQRTLGYLAMLFVVLLIGLPLFWMLSGSLKTTQEIYSTDTQWI
                     PESPLWSNYRDAWERAPFGRFYINSTIVTLLGAGLEIVNATLTAYALAYLRFPFKNLV
                     FILILAALMIPPQVTILPNYVFFGLNGVIGELFGESWINTYQAIILPGASVAFGTFMM
                     RQAFLGLPREVLDAAKVDGAGHLRILRDVVLPMAKPVIVTFALISLVAKWNDYLWPLV
                     ITQTQNMRVLTIGISYLFDAEGNNEWGIIMAATFFVVFPLLIVFLFAQRFIIEGLTAG
                     ATKG"
     gene            13992..15329
                     /locus_tag="VGR22_03320"
     CDS             13992..15329
                     /locus_tag="VGR22_03320"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2127631.1"
                     /translation="MTTTRINRRSMVKGAAGVAASAAIGSKASSVFAAPAVIQSGPIE
                     VTYWTSFQGGANGEAQDEVVRRFNESQSDIRVNHEFQGSYEETAQALTAAVQGGSAPD
                     MSLLSDVWWFKFYLSQLLQPLDDYIAAENVDTQDYVDSLWNEGVRQGVQYWIPFARST
                     PLFYYNAEVLEEAGLDPSIFQNWDTIVENTEALMARDGDQVSRYAFGHPNAASYIAWL
                     FQGVIWQFNGEYSQPDFTITIDEPAGIEAGNFYRDSVADGWAGASAEHDVDFATGLYA
                     SVMASTGSMGNIRNTANFEWGTAFLPEKYTFGCCTGGSGLSVMASADDANKEAAFEFI
                     KFSTSPEITAYWAQQTGYMPVRKSAVESEEMQAFYEENPHARTAVEQLPLTEPQDAAR
                     VFIPNGDQIIGGGLERITVNTEDAGEVFPDVAQTLEEEAEPVVQQLESVEPEG"
     gene            complement(15431..>15762)
                     /gene="tsaE"
                     /locus_tag="VGR22_03325"
     CDS             complement(15431..>15762)
                     /gene="tsaE"
                     /locus_tag="VGR22_03325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEV2127632.1"
                     /translation="IESAVQSPTFTLMTEYAAAVGDRSPGTLYHLDLYRLDDPQELGE
                     IGWEEALAPEQGVTVVEWPERAGDWLPDRFVLVSIAYRDPGSRQVTITSMPPDRAGEL
                     TASMEMG"
BASE COUNT         2773 a         5397 c         4794 g         2798 t
ORIGIN      
        1 tgagcccgat tcccggagga gcggctgtcc gcgagggatt gatgtacgtc gtcgcccggc
       61 tctccggtta cagccccgag gcggatgcga tccttgcggc agcgctcatt taccgctttg
      121 cgctctttgc cgccatcccg atactctttg gcctgaaccg cctttggctg aactccagcg
      181 ggaatccggg catcaacggc cgtgaggttc ccgccgacga aaggatctga accggatgag
      241 catcagcgag acttacctct acaaaccacc ccaggcagta gagacgagca acttcgcgaa
      301 gcacaccagc gacaacccgc tccagcgacg gctgatcgat cgttttcatg cgaagctcac
      361 cgagctggtg aaggcggaag acccggagac gctgttggac gccgggtgcg gtgaggggtt
      421 caccgacgag atcttcctgc aggccatgcc agcgctccag ctgaccggct tcgaccccca
      481 cgagcccgcg atcgaactgg cttgcatgcg aaacccgcgg gcggagttcc tcgtcgccga
      541 tatctacaac atgccgttcg aagacgacag cttcgacgtc gtatgctgct tcgaggtcct
      601 ggagcacctg catgacgccc cgcaggcgct ctcgaagctt gcccgcgtcg ctcggaaagc
      661 cgccgtgctg agcgtgccgc acgagccgtt cttttgcctc gcgaacatgg cgcgcggcaa
      721 gaacctcgac atcgacccga agggcagcga ccccgaccac cgcaacttct ggtcacggga
      781 ggcgtttggt gcgttcgtgc aacgcgagat ggacgtcgcg cacctgaccg gctccttccc
      841 gtggtccatc tgcgtggcgc gcccgcgata gctcggtaac cgtcgagcac ccgtaccagg
      901 cccggtgacg tacactccgc catgacggac ggaggtcaac gatatcgtgc ccgacactcc
      961 atttcagcat cccccctcca ccactccgtt gggcgggagt tcccgtcgtc acgaacggcg
     1021 cagcaatttc gcccgcaact ggacgggcta cgtgcctatg gtgcgcgtct gtcccgcctg
     1081 tgacacggaa aacgatcagc acagccggtt ctgcgtcggt tgcgccgcca acatcgacga
     1141 tgtcgagccg acaccctctc ggaatcccaa cagcggtgtt cgagcgatgg aggcgcgcat
     1201 ccggcgtgaa gcagacgtcg cccgtcgcta tcggcccttt accgctgccg gaggcaccgg
     1261 cttcatcatc acgggcgtga tgctcctgct cgccgcaacg atgttcccgc tgccattctg
     1321 gattcgcctg ggcacctggc tggcaggcct cgccttcacc gccgcaggcc tctggcgcat
     1381 gcgcttcgat ggcgacgcaa ttcggcgtac cggcttcgtg cttgcggcct cgacgatcgg
     1441 catcgtggcc ctggtcctga cccgcggcac cctgccagcc gatccggctg cgctcctgcc
     1501 gacccctcaa ccgacccagg tggcgatgca ggccacccct cctcccgcat ctcccgttgc
     1561 gggagcgacg ctcaccggtt cggtcaccac cttgctcggc ggtccatcgc actctggcat
     1621 tcaggacggt ccgccaccga gcggcaaccc ggctctggcg tggcgcttcg acaccggcaa
     1681 agaaattctc tcgcaacccg tcatcgccga cggcaccgtc ttcatcacaa accgtgcggg
     1741 gttcctctat gccatcgatc ccctgactgg gcagcagcgc tggcgggtcg agctggggga
     1801 ctacgcgctc cggacgaccc cgacatggca cgaaggtcgc ctctatctcg tctccggctt
     1861 tgaggcgatc gcgctggacg cccaaaccgg cgagaccgtc tgggagacta cgatccgcta
     1921 cgccggaaca gcctcgccga ccgtcgcgca cgggaacctc tacgtcgtca gccaggaggg
     1981 atggctctac gcgctctcgc tggagaacgg cggtgaagtc tggaaggccc cgacggatgg
     2041 catcagcttc ggcccgccca gtgcgacgga gcagtacgtc gtgatcggca ctgacaatgg
     2101 caaggtcaca gccttcactc cggaggccgg ccgacaggca tggcgaaggg atttcgaatc
     2161 ggccatctac agcccaccgg tcatcgtcga cggcaccgtc tggatcgtta cggacgacgg
     2221 tcggctgctg gggctcggcc tggacgacgg aggcgaccgg ttcgcgctcg aggcgaccgc
     2281 cgatcttccc gtgacagctc gtgacgccac agtctacgtc ccatcaactg atggcgggct
     2341 gtacgcgatg gatgccgaga gcgacgcggt gttgtggttc gcgtctgcgg gtggggcggt
     2401 acgcagcggg ccggtccaga gcggtggcca agtggtcatt tctgggggga atcgcattgc
     2461 cgggctggac cgggagagcg gcgagcagat ctggtactac cttgccgggg acagcattgt
     2521 ggcacccccg gcaatcgtcg acggccacgt cttcttcggc gcccgggatg gtgtccttta
     2581 tgccgtcacc gaccggggat ccccgtgacc acgaaccggt agaccggccc cgcaagcggg
     2641 cgggagttcg caggcaccat cgacgcagca agtacccaaa ccaggtccat cctgtccatg
     2701 tagaaagacc cgcccattcg tggggcgggt ctaccggttc agcaccattt gcacagatgt
     2761 ccgtccccgg caacgaagca gatttgcact cgaagtacga ggggacgacc tgtcggctac
     2821 gccgccatat ccttcatgat ctcggctgca gcctcgacgg ccagatcgat gtcccgctca
     2881 ctgtgcgcgg atgacaggaa gagcgcctcg aaacgcgatg gtgcgaaata gactccgcgt
     2941 gtgagaagcc cctggaagaa cttcgcatag cggcctccgt cgtcgaccgc cgactccgca
     3001 tagttcctca ccggcgcatc cttgaagaag acgccagcca tcgtcccgat cgcgggcgcc
     3061 tgcaccggca cgcccacctc gactcctgcc gatcgcaggc catgaaccag ctgctcggcg
     3121 cgatcggcgg ccgtcgccca aacgccttcc tcggccaaca cttcaagggt cgcgatcccg
     3181 gcagcagtcg ccactgggtt gccggagagc gtgcccgcct gatacatcga gcctgccgga
     3241 gcaacatgct ccatgatctc ccgcttgccg gcataggctg ccagcgggaa gccaccgccg
     3301 acgaccttgc cgaaggttac gatgtccggc tcgatgccga accgctcaac ggcaccgcca
     3361 ggcgcgacgc gaaagccggt catcacctcg tcgaagagca agagcgctcc atgcttcgcg
     3421 gtgatctcgc gcaggccctc gaggaagccc tcaaccggct cgaccatgcc catgttgccg
     3481 gcgaccggct cgacgatcac cgtggcaagc tcgtcgccat actgatgaaa aagtgcccgc
     3541 acgctatcga ggtcgttgta ggtggcgacg agcgtgtcct gcgtggcgcc cttcggcacg
     3601 ccgggactat cgggcagccc gagggttgcc acgccgctgc ctgcctgaac cagcagcatg
     3661 tcaccgtggc cgtggtagca gccggcgaac ttcacaatct tgtctctgcc ggtggcggca
     3721 cgcgcgaggc gcagggcgct catcgtcgcc tcggtgcccg aggagacgag ccgcaccatg
     3781 tcgacgctgg gcacgcgctc aacgatcagc tcggccaggc gcgtctcggc gaccgtcggt
     3841 gccccaaagc tggtgccctt gcggagcgca ttgctcgtcg cctcgacgac cttcggatgc
     3901 gcatgtccga gaatgagcgg tccccaggag aggacgtagt cgatgtaggt gttcccgtcg
     3961 atatcagtga agcggcttcc ggagcccgac tcgacgaaga tcggtgtccc gccgacagcc
     4021 cggaaggctc gcaccgggct gttgacgccg ccgggcatca cctgctgtgc agtggcgtag
     4081 gcctcggcgg atttcgcgta gtgcggcgtt gtgcgggttg tcgattcggc aggcatggta
     4141 ttcccctgtg acttcatcgg cacggttcgc tttctcgatc gcagccgttc agcggtcgtc
     4201 cggctcgtct gggtcgacgc cgagatgcgc gaggatcgag cgtatctcga gctcgctgcc
     4261 ggcgttgcgg agcgactgca caggctcatg catgagcgtg ttggtcagcg ccttcgccat
     4321 cgcacggacg acgttctgat cgcgctcgga caggtggccc agccgccgca gcgctcgctc
     4381 gacctcacga tcccgcacag cgtcgctctg gtgacgcagc gcggcgatgg catcggaagc
     4441 cgttcggctc ctggcccact ggaggtagtc gtcaatggcg cggtcgacga gctgctcgac
     4501 cttgttcatc tcgttcgagt agcgctgtcg cgactcatgg gcgacggatt cgagatcgtc
     4561 gacatctcgt gcctcgacct gcgctacgta gccgcatcct ctatcgacga tccggggcac
     4621 gccgagatcg atcagcagca gcggcttcgt gcgcccctca agcatcaggg gaacgatgac
     4681 gggctcctcg gtgaggatcg ccgcaatcac cacatcgctc tcggcgatcg cctctcgcag
     4741 ctccgtcgtt ggccgagccg tggcgccgat ctgggcggcc agctgctccg ccttgccaac
     4801 cgagcggttg acgatcgtca gttgcgccac tccgcgcgct gccagcaccc gcgcggcgat
     4861 cgatgccatc ttgcctgcac caagcagggt cactcgctgg gtggcgagat ccggccgctc
     4921 ccgctcggcg agcgcgacgg cggcgtgact gatcgagagg ttgttccggg cgatgtctgt
     4981 ttccgtgcgc gcctgcttgc cgaccttgag cgcgtcagtg gcgagacgct ggaggtaggc
     5041 gccagcgctc tccgcagttc gagcggcttc cagcgcgtca cggatttgcg cgaggatctg
     5101 cggctcgccc agcacaatcg agtcgagccc gctcgccacc cgaaagatgt gccgaactgc
     5161 actcgcgtcg gtatgtgcgt aggacatcga atcgagtacg tgacgcggca tgccgtggta
     5221 gctcgacagg aaatcgatga tgcgctcgcg cccctgctcg ccgtcctcgg cgactgcgta
     5281 aagctccgtg cggttgcagg tcgagatgat cagcgcctca tcgacatgct ggcgcaacgc
     5341 acgaagcccc ccggtcagcg tctcgccatt gaaggccagc cgctcccgga ggtccaccga
     5401 cgcacattcg tgattggatc cgacgatgat gatggagggt ttcgacacga ttccgttcac
     5461 tgtgacatgc agcgcgtgac ggtccggagg cacgatgtcg ccggggtcgc gctcctcgta
     5521 tagcatcaat cttaccgcat gggcaccgcc gggcaggtcg tcggagacat ccgatcatgc
     5581 catcatgccc agcctcgcag ggcttttgca gcattctcga atggcccata tagtgcccac
     5641 agcgacacac ggcgcccagt tccgtacgga catgctgcag gcgccaatca tcagaaccag
     5701 gagaatcccc cggcatgcca gttcgcacca ttcacgagga ctatcacgag gaattcgccg
     5761 catcgaaggc gctctgggag cgctcgagcg agtcgatcgt cggcggaatc actcacgacg
     5821 gccgcttcat caagccgttc ccgccctacg tagcgagagc cgacggcggg cacaagacct
     5881 cggtcgaagg caacgacctg atcgacttct gtgtcgggca tggctcggtc atcttcgggc
     5941 acaacgatcc ggacctcacg gctgcaatac aacgacaagt gccgatcggc acgcacttca
     6001 gtgccggtca tgagggtgag gtccgctggg cggagcagat cgtcaagctg gtcccctccg
     6061 cggagaaggt acgattcacc gcctccggca cggaagcgac gctgctggcg atgcgcgtag
     6121 cccggggcta cacgcagaag aaaacgattc tgaagttcga ggggcacttc cacggctggc
     6181 aggactacgc gctcaaggga gagaagccgc cgttcgagaa tgtgagcgta cctggcgtgc
     6241 cgtcggagac gctcggcacg gtgagtgtcg tgccatcgaa tgacctcgcc atgctcgagg
     6301 aacgtctggt ccagggtgat gtcgcgggcg tcattctcga accgagcggt ggctcctaca
     6361 cgacgatccc gctcgccgac ggattcctgc agggggtgcg cgacctcacc gccaagcacg
     6421 atgccgtgct gatcttcgac gaggtcatca ccggattccg ctggtcgccg ggcggtgcgc
     6481 agcagcgctt cggcatcacg ccggacctca ccacgatggc aaagatcgtc gccggcggca
     6541 tgccgggcgg cgcggttgcc gggcggcgcg acatcatgga cgtgattcgc ttcaccgacg
     6601 acgccgcctg gaactcgaag cgtcgcgtcc cgcaggccgg cacctacaac gccaaccctc
     6661 tcgctgcggc ggcgggctat gcctgcctcg tgaagatcga cgaccaatcg gtccacgacc
     6721 attgcgacca gatcgccgcc gacatccgca gtggaatgaa ccgcgtgctc gagaagcacg
     6781 acgcgccagg ctttgcgtgg ggcgagagct cagtcttcca cgtggcgctc gacaagcggt
     6841 gcactaatcg atccggcgat gacctgcgca cgccggaggg ggtcgatccc gagtacctca
     6901 agaagagcgg agggtcgccg ctcggcatgt tgctcgaggt cggcatgctg ctggaaggtg
     6961 tacacctgtt ccatgccggt ggcctgacgt gcacgaagca tgacaccacc gacgtcgagc
     7021 gcacggtcgc ggcgttcgac caagtggtcg gccgcatcaa ggatgaaggc cacttcgacg
     7081 gcagctagca gcctgcccgg tcccggccca cgatcagccc caagatgcct gcaaccgctc
     7141 ccagtcctcc tcggtgtcga cgtcgagcgg tgggccaggg agcggcacgt cgacgcgctc
     7201 cacctgatca ccgagcctgc gcagcacctc cctgccggca gagtcgcctt ggagacgcgc
     7261 gagatcggtg aaggccgagt ggtcgaagag cacgggatga ccgtcaccgt cagtgtaccg
     7321 agccatcacc gccggcgcgc gagtctgccg ccaccgctcg acgactgccg cgatcgcctc
     7381 ggtcctgatg cccggcatat cggcaaggag aacgacgata gcgtcaacgg tcgacggtat
     7441 cgcgcgaacc cccgccgaaa gcgaatttcc ctgccctttg ctgaaatgcg ggttgaacac
     7501 gcggtaggtc tggtaatcga gcagcgcctg ctcgacctcc gaagcgtgtg agccgatgac
     7561 aaccaccaca ccgtctatag gcgccgccag ggcggcctca accacgtggc acaccagcgg
     7621 tttcccaccg agctgcagca gctgtttcgg ccgcccgagc cggcgggaca gccccgctgc
     7681 aaggaccagt gcgaacacgc ggtcgttact caccccgtat cgcgcccttg atctccgtga
     7741 gcggtctgcc gtcgcgcccg tgcgtcaccg cgatcatctc cgcgaggatg ctgaccgcca
     7801 tctcctccgg tgtggatccg cccaggtcca gcccgatcgg cccacgcaca cgcgcaatct
     7861 gctcctcgct cacgccggca tccaacaggc gttgccgccg atcggcgttg gtcttgctac
     7921 tcccaaccgc accgatgtat cgggcccccg tctccagtgc tccgagcagc gccggctcgt
     7981 caaacttcgg gtcatgcgag aggatcgcaa tggccgtatt cggagtcacc gtcaactgct
     8041 cgtaggcgtc cgctggccat gcgaccagga gttggtcagc gtcggggaag cgttcgcgtg
     8101 tggccaaggc gcgccgggca tccaccacca ccacgcgata gccgagcctg cgggcgaacg
     8161 tgtccagcgc ctgcgcgatg tgcacggcgc caaagatgat gagctgccgg ctcggctcga
     8221 agacatcgag aaagatccgc gttccctcgt ccacctccag cagcccggac gccggtgttc
     8281 cagcgctcgt gctcaccgct ctgagggccg actcatccag cgcctccgac cccaacgatc
     8341 ccatcgtgat ctcgccggac gtgatcagcg ccttgctccc gaccaggtcc ccttcgacca
     8401 ccgtcgccac aacggccgtc gtaccctcag cggcgagtcg gcgcgcctca tcgtagaccg
     8461 cacccgagtc cggcggtgcc gccatcacta cgcgtccatc ggctcgataa agacctcgat
     8521 cgtcccaccg cacgacagtc ccacatccca cgccatatcg tcggagatgc cgtactcagc
     8581 gagccgcggc gtattgtgct tgagcacctg ttgcgcctcg agcaccaccg acgtttcgac
     8641 acagccacca ctcaccgacc cggccatctt gcccttttcg ttgatggcca tgcgcgcccc
     8701 gacgggacgc ggtgccgagc cgatcacgtt gaccaccgtg gcgagggcga ccttcttccc
     8761 ctcggaacgc cactgatcga tctccggaag ccactcagcc atcgatcgct cctttctctc
     8821 tcttttctcg ctccgctgcg cgtttggata ccacccggtg cgacgcccgc ctcggcggct
     8881 gctcgtcggc ctcgccgagt agcgcggcga gcgctccgag gctctccagg ttgtgcacag
     8941 gcaggaagtg atcgatatag ggcagcgccg cgcggatacc ctgtgtcagc ggctggtatc
     9001 ccgccgcgcc cagcagcgga ttaagccaga tcagccgacg gcaggaccgc tgcagacgcg
     9061 ccatctcgtc gccgagcaaa tcgggaatac cacggtccca accatcactg atgaccacca
     9121 cggtcgcgcc ggagagcaac acgcgacggc tccactcaaa gttgaacgtc ttgatcgcct
     9181 caccgatacg ggttccaccg gaccaatcgt cgacagacgc caccacgtcc tcgatcgcct
     9241 ggtcgacgtc gcgcttacgg agctcccgcg tgatccgggt cagccgagtg ccgaacacga
     9301 agacctcgac cgaatcaagc ccgtgctcga gcgcgtgcac gaagcgcagc agcatgcggg
     9361 agtagcgatc catcgagcct gaaatgtcgc agatcagcac caacggccgc atgcgctgct
     9421 tccgggtgcg gaacctgaga tcgatcggaa cgccgccctg cttcatcgac tgccgcaggg
     9481 tacgccggta gtcgatgaag tccccgcgca ccgccttctg ccggcgtcgc gtctcgcgcg
     9541 tgctcagacg ccatgacatc tccgccatga tgcggcgcgc ctcggcgatc tcctcgtccg
     9601 taaactgcga gaagtccttc ttgcggaacg cttcgctggc gctgaaaagc agcacatcct
     9661 cgggcggggt ctcgtactcc tcacgctcac cgatgtcacc ctcgcggtcg acgccttcga
     9721 tcgcaagcat ccgccggtcg gactcctgcg cctctcccga ctgcccgtgc ctgctctcgc
     9781 ggtcctccgg cgtgtcaccc cgcttcggct tgcccgtccg ctcctcgtcg ctggagaggt
     9841 actcctcaag cggcatcatc cgctcctgga gtaggtcatg ccagaactgc tcgaactcgg
     9901 ccctgaacgc actgagctgc tcgggccggg tcacgagggt ggcacgcgcg gcgtggaacg
     9961 cgtcgtcgta ccgttcaagc gagacctgct caagcgcggt tacgaactcg aggatctgtc
    10021 ccggtcccac gttcattccg gccgcccgca gccgccgcgc gaagcgcagc aggttccgcc
    10081 ggatagtggc ggcgttgacc tggggctcac gcgagcgctg attgtgacgc tcgctgtact
    10141 ggctaagggg ttggctgtcg tgttgcatcg agtgcttccg caacgagccg ctgggcgacg
    10201 tcgccggtga tcctgtcgac atcttcctga tacttgagaa tcacgcccag cgtctcctcg
    10261 actacctcat ccgaaagctc cctgctgcca agcgcctcca gcgccgccgc ccagtcaagc
    10321 gtctcggcca tccccggcga cttgaacaga tcgacgtcgc gaaggccctg agtgaaggtg
    10381 cagatctgcg tgacgagccg ctcgcttgct tccggcacct tcgccgtgag aatccgtgac
    10441 tccttctcga aactggggaa gtcgatccag tagtagagac agcggcgctt gagggcgtcg
    10501 tggatctccc gggttcgatt ggaagtcagg atcacgatcg gtacatgctc ggcacgcagc
    10561 gtgccgacct ccggcacggt gacctggaaa tcggagagta gctccagtag gaaggcctcg
    10621 agctcctggt cggcccgatc aacctcgtcg atgagcagca cgggcgcctt gtcgtgggca
    10681 acatcgatcg cctgcagcag cggccgcttg agcaggaact cctcgctgaa caggtccttg
    10741 cgcacctcct cgcggtcgag atcccctgtg gcctccagtg atcgcaggaa gagcatctgc
    10801 ctcgggtagt cccattcata aatggccgag ttcgcgtcca gcccctcgta gcactggagc
    10861 cgaatgagcg gcgtgtcgag caggtcggcg agcaccttgg cgatctcggt cttgcccacg
    10921 ccggcctcac cttcgagcag cagcggcttg cgcatcgtca gcgtgagata cagcgtggtc
    10981 gccaggccgc ggtccgtgac gtacttcttc tctcgcagcg cctcctcgag ctcgctcacc
    11041 gacccgaagc gccgctggac aacttcattc actgtttcca tggaatcctg ccgttcgatc
    11101 gtgttcgtgc tcaacaatgt gcctcgcttg agaatcgaac atactggctc gggccgctca
    11161 gccacgccgt ttgcgcccag tatcaggcac ctcggcgatg ctggccacac ccgcaactac
    11221 ggtcgagcgt cgcgtacggg acgcgatgtc ttcgatagtc cgtggacgta gagcggctcc
    11281 agagtcacga ggtcatcggc gtgcccatgc cggagccgat gcgctccgag cagtgcgatc
    11341 gccccgatcc ggctcgtcaa cccgggctcg ctcagcaccg gcaccgccgc gcctgacagc
    11401 gcttcgcgaa gagccaccgg cagctccccc accatggcgt ccgggcgggc gccagcgagc
    11461 gaggccagca gctcagcggg cgtgccgttg acaggaatcg ccgatgggcc tccgggagag
    11521 atgctctgcc acacgaggcg cccacggccg gcggccagca tcgccaccac cgaccgattg
    11581 gcctgcgccc acggaagcgc gatcgcgtcc aacgtgggca ccccggcgat cggtatgccg
    11641 agcgcgatcg ccagcccctt ggcgatgctc atgcccactc gcaggccagt gaaggtgccg
    11701 gggccctgtg caaccaccac gccgacgatc tccctgacgt cgacatcggc tgaggccagc
    11761 aggtcgtcaa tgcgcgacat cgcctccgta gtctggcggc ggtccgatgg ccacaccatc
    11821 tcgtgcagcg ccgtgccgtt ccagagggcc accccggcac gctcagctga ggtgtcgatc
    11881 gcaagcaccc attcagaaga ggcacttgcc gtgacgcggt cgtaagaacc aaggtaggat
    11941 gttagcattt agatagccat gttcctttgc ttcggtcgta gactagccgc ggtggccctg
    12001 agcgttgccc tgtcatcggt cgaaatgtgt atgcctactg gcaggagttg tgccgatcgc
    12061 acgcaggctc gcaaaccctg caagccacga tgggacggga atgggagaca taggcaagca
    12121 tctggtcgca ccccctcgtc agcaggcgta tgaggtcgcc aggccacggc gcaactggaa
    12181 catcctccgg aaagaatggg tgctcttcat cctcttcgcc ggtccgaacc tgtttctctt
    12241 ctcgctcttc tcctactggc ccatgatcga gaacatgcgt ctcagcaccc agcgctggga
    12301 tctgctgtct cccgttcgca taaacgtcgg gcttgacaac taccgatacc tgattgcgaa
    12361 cgacacgttc catcaggtcc tctggaacac actctacttc acggttgccg cggttgggct
    12421 ctccctgctg ttgggcctgc tgatcgccct gctgctgaac cagcgcctgc gttggcgcaa
    12481 cggcgcccgc gcgatcgtct tcgcccccta catgctctcg ggcgcggcaa tcggcatcgt
    12541 atgggcctac atcttcgacc cgcgcttcgg ccttctcgcc gagatgcttg gctgggtcgg
    12601 cctgaactcg ccggactggc tcaaccggcc cgagtgggcc atgccggcca tcatcattgt
    12661 ctacgtctgg aagaacctgg gcttcaccac ggtcatcttc ctggctggac tccagtcaat
    12721 cccgcaggac ctctatgacg ccgccaagat cgacggcgcc aatgtcctgt ggcgattccg
    12781 gtcggtcacg ctgccgatgc tgtcgccgat cgcgttcttc ctcgtcgtga cctcgatcct
    12841 gagcaccttt cagtcattcg atatcatcag cgtgatgacg gacggcggcc cggtcgacgc
    12901 aacgaacacc ctcatctact acgtctacga gcagggcttc gtcgccttca acgccggccg
    12961 gtcggcagct gcggcgctcg tcctctttgc catgatgatg gtgatcaccc tgatccagct
    13021 ccgatactcg gaaagcaagg tgcactatgg gtagcttgag ccaacagcag caggtaatgc
    13081 tgcagcgaac gttgggctac ctcgccatgc tcttcgtggt gctgctcatc ggcctgccgc
    13141 tgttctggat gctttccggc agcctcaaga cgacacaaga aatctactcg accgacacgc
    13201 agtggattcc ggagagcccg ctctggtcga actaccgtga tgcatgggag cgagcgccct
    13261 tcggtcgctt ctacatcaac agcaccattg tgaccctgct cggcgcaggc ctggagatcg
    13321 tcaacgctac cctgacggcc tacgctctcg cctacctcag attcccgttc aagaacctcg
    13381 tcttcattct catcctggcc gccctgatga tcccgccgca ggtcaccatc ctgcccaatt
    13441 acgtcttttt cgggctcaac ggcgtgatcg gcgagctgtt cggagagagc tggatcaata
    13501 cctaccaggc catcatcctg ccgggggcat ctgttgcctt cggcaccttc atgatgcgac
    13561 aggccttcct cggcctgcct cgcgaggtcc tcgacgccgc caaggttgac ggcgccgggc
    13621 acctgcgcat ccttcgcgat gtggtactgc ccatggcgaa gcccgtgatc gtgaccttcg
    13681 cgctcatctc gctcgtcgcc aagtggaacg actacctctg gccgctggtc atcacccaga
    13741 cccagaacat gcgggtactg accatcggca tctcctatct ctttgatgca gaagggaaca
    13801 acgagtgggg catcatcatg gctgccacat tctttgtcgt gttcccgctg ctgattgtct
    13861 tcctgtttgc ccagcggttc atcatcgagg gcctgaccgc cggtgcgacc aagggctagc
    13921 gccatcgcca gcgcaggcgt ggcaaatcgc tatacggtgc cggattccgg catgcaagga
    13981 gggtcgacag aatgaccacg acgaggatca accgccgttc gatggtgaag ggtgccgctg
    14041 gtgtggccgc ttcggcagcg atcggctcaa aggcatcaag tgtgttcgcg gcgccggccg
    14101 tgatccagag cggcccgatc gaggtcacct actggacctc attccagggc ggcgccaatg
    14161 gcgaggcgca ggatgaggtc gttcgacgat tcaacgagtc gcagagcgac attcgcgtca
    14221 accacgagtt ccagggcagc tacgaggaga ccgcccaggc gctcaccgct gcggttcagg
    14281 gcggcagtgc gccggacatg tcgctgctct ccgacgtctg gtggttcaag ttctacctga
    14341 gccagctcct ccagccgctc gacgactaca tcgccgcaga gaacgtcgat acccaagact
    14401 acgtcgacag cctctggaac gagggcgtgc gccaaggcgt ccagtactgg attcccttcg
    14461 ctcgctccac ccccctcttc tactacaacg ccgaggtgct tgaggaggcg gggctcgacc
    14521 ccagcatctt ccagaactgg gacaccatcg tcgagaacac cgaggcgctc atggcgcgcg
    14581 acggtgatca ggtcagccgg tatgccttcg gccatccgaa cgctgcctcc tacatcgcgt
    14641 ggctgttcca gggtgtgatc tggcagttca acggcgaata ctcccagccg gacttcacca
    14701 tcaccatcga cgagcccgcc ggcatcgagg ccggcaactt ctaccgtgac tcggtcgcag
    14761 acggatgggc cggcgcgagc gctgaacacg acgtcgactt cgccaccggg ctctacgcct
    14821 ccgtcatggc gtcgaccggc tcgatgggca acatccgcaa taccgcaaac ttcgagtggg
    14881 gtaccgcgtt cctcccggag aagtacacct tcggctgctg caccggcggc tccggcctgt
    14941 ccgtcatggc cagcgccgac gacgccaaca aggaagcggc cttcgaattc atcaagttca
    15001 gcacctcccc tgaaatcacc gcatactggg cacagcagac gggctacatg ccggtccgca
    15061 agtccgccgt ggagagcgag gagatgcagg cgttctacga ggagaaccca catgcccgca
    15121 cggcggtaga gcagctgccg ctaaccgagc cgcaggacgc agcacgcgtc ttcattccca
    15181 acggcgacca gatcatcggt ggcggccttg agcggatcac cgtcaacacc gaggatgccg
    15241 gggaagtctt cccggatgtc gcccagaccc ttgaggaaga ggcggagccg gtcgtccagc
    15301 agctcgagtc ggtcgagccc gaggggtagt ctctccagcg ctcagcactc gactgctcct
    15361 gtaatgcgag caaaccgggg gtggcgacca accatccccg gtttcgtcac cgcgctatgc
    15421 gaggccggca tcagcccatt tccatcgacg ctgttagctc acccgcgcgg tccggcggca
    15481 tgctggtgat cgtcacctgc cggctacccg ggtcccggta cgcgatcgag acgagcacga
    15541 atcgatccgg cagccagtcg ccagcacgct cgggccactc gaccacggtg accccttgct
    15601 ccggcgccaa cgcctcctcc caaccgatct cgccgagctc ctgaggatca tcgagccggt
    15661 agagatcgag gtgatagagc gtccccgggg agcggtcgcc gactgctgcc gcgtactccg
    15721 tcatgagcgt gaaggtcggg ctctggacag cgctctcaat tc
//
LOCUS       DASYWL010000087        10916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_22236, whole genome shotgun sequence.
ACCESSION   DASYWL010000087 DASYWL010000000
VERSION     DASYWL010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10916
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22236"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            302..2518
                     /locus_tag="VGR22_03330"
     CDS             302..2518
                     /locus_tag="VGR22_03330"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV2127633.1"
                     /translation="MSEQDKDRKTVPENQQGQGRSRRRRRRRPQGAQGGNQQQQGQDR
                     TQQSAGAESDEARASSGQRRRLRGGRGRGGPSQQQDQQGGNQQARQQGGRRGGQQVAS
                     SEQQAQEKGQGQRRRRANRRPRREEHAPERVEPRREPHERARGRQAGGGRGQGPAPIL
                     GERMRIIGAPRTGAVPAPIGKDRLRVIPLGGVGEVGKNMTAVESGKDIILLDAGGKFP
                     EEDQQGIDLIIPDVTYIKERRKNFRGILLTHGHEDHIGALPYVIPQLYEGKGSPKIPI
                     YGTPLAIGFVERKLLEARVDDMVELKTVESGERVKLGENISAEFIHVTHSIPDATSIA
                     VHTPVGTIVDTGDFKFDPTPVMGDPADEKRLRKLGDNGVLALFSDTVRVESEGSTPSE
                     KVVLETIDDVIGHAAGQVIIATFASNVSRVLMALKAAEKHGRKVAVAGRSMEQNTRVA
                     LDLGYLDPPEGLLVTLDEVMRLPKNKRVIVSTGSQGEPAAALARIAAGEHPKIRVGRG
                     DLILVSATPIPGNEDTVSRTIDNLFQQGAEVVYSAIDRGVHVSGHAGQDELRRMLEFI
                     RPTYAVPVHGEYRHMALYRELAVKSGVKRENVLLPEIGGVIEFTKSSANQRARVPSGN
                     VLVDKLGERDGVNAVLRTRENLTEEGVVVVSLVVSRSNGELISGPDITGQELGKELSN
                     GALREAERELKRSLTRRRKGAPQYGFLAQRVKESVAETLYRRTRHRPLILPVIAEL"
     gene            complement(2836..3732)
                     /locus_tag="VGR22_03335"
     CDS             complement(2836..3732)
                     /locus_tag="VGR22_03335"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEV2127634.1"
                     /translation="MPIPDERLGNPPVDLLWILAHPDDESFGSAGTMAWANGAGLRTA
                     YVCATRGDVGEIRDSHLATRDTLAAVREQELREAMSHLGLGELRILGFRDSGMENTSE
                     NEHPLALINQPIEALLTHLVGHIRDLRPDTIITFGPEGIYGHPDHVLMGKLTTRAVEL
                     AADPSWLPALHEPWCANALYFASVPRELILAIACDPAQPLGEISEKASNNLGSPASTI
                     THWLDVSRWLGAKRQAIIAHRTQIDPDEIMNPESQLAQWLHREQYTRRPLPWDPGMAN
                     EDPLTRARDEFGSISPPAGAIP"
     gene            complement(3763..5316)
                     /locus_tag="VGR22_03340"
     CDS             complement(3763..5316)
                     /locus_tag="VGR22_03340"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEV2127635.1"
                     /translation="MQQNRLAFEPGHAETTWDAAQPPLVLAVDIGSSSVRALMFDAHG
                     LQIDTSEHQIPHRLHTTADGGAEADPHDLFQLVIECIDQVLDLAGEHASDIGAVATTS
                     FWHSLMGVDAAGSPTSPVYMWGDKRSGDEVPRLRESLDARQLHRETGCRFHSSYWPAK
                     LLWLRQERPAIVADTRTWCSFADYVDRRLHRCMQTSVSMASGTGLLDVEASSWHCELL
                     EVLDLDEDRLPALVDRDEPLPLLTGAWQSRWPALAEVPWFPAIGDGAAANLGTGAVGK
                     DRVAITIGTSGAMRIITEDATTPTMELSARIWSYRLDRSHRVTGGALSNGGNVTGWLA
                     RYLAGGNFDILTAEANRMAPDSHGLTVLPLFAGERSPSWNDDASGVFAGLTLATRPGH
                     LYRAVLEGTAYRFASIHEALLPMLEQDHEIYVNGAAALKSPLWLQIVADTLGRPLSAL
                     DAEAEGSARGVAVSALEAIGAVPSLRPESPAVAATYTPVAEATPVYRAGQERQAALEQ
                     AILNFWAPT"
     gene            5463..6239
                     /gene="cofE"
                     /locus_tag="VGR22_03345"
     CDS             5463..6239
                     /gene="cofE"
                     /locus_tag="VGR22_03345"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEV2127636.1"
                     /translation="MSTVSPISVMPVAGLPEISPRDPLGRMIAERIEEEIGGLQAGDI
                     VVVTHKIISKAEGQLVDLSTVSPSPLAVQLAERHGKDARQVEMVLRESARIVRMSGGH
                     IISETHHGLICANAGVDASNTDVGVVSLLPRDPDASAARLREHLVRDVPAEDSERIGV
                     IVTDSFGRPWRNGIVNVAIGVAGISPLADYRGQFDAAGHELRSTVLAVADELAAAAEL
                     VMHKTQAIPVAVVRGYSWQIEGQPGTGQDLIMPVERDLFR"
     gene            6275..7270
                     /locus_tag="VGR22_03350"
     CDS             6275..7270
                     /locus_tag="VGR22_03350"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEV2127637.1"
                     /translation="MTQGVVGYAASFEQFHPNELLAYSQQAEQQGFGAVMASDHFHPW
                     VPSQGHSAFVWTWLGSLGTATSIPKFGTGVTPPGYRYHPAVLAQAAATVEAMFPGRFF
                     LGLGAGEALNEHIVGEYWPEAPIRLERLMESIEIIERLFEGKVVKHQGKHFRVESAKL
                     YTLPEKAPPIYVATSGPIMAKRTGKHTDGIITVGAADEKLKMLMDRFEKGAQEAGKDP
                     KSMPRMLQIKVSYADTDEEAIDSAVRDWPNGGMSFPKADVRNPDDFEAMAKLVRPENF
                     KNRVLTSSDLDAHVEYIQRFIDLGFGEIYVHNVNRDQEKFIDAYGKHVIPNLNWK"
     gene            complement(7395..8072)
                     /locus_tag="VGR22_03355"
     CDS             complement(7395..8072)
                     /locus_tag="VGR22_03355"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEV2127638.1"
                     /translation="MSAQSTSAPATENASTTGPSRAQQVGIVAAVALLIIASGVWLAR
                     SQGASELGSGGVNSSLLPKVGEPAPEFMTLYADGEVFRLSDLRGEGVWLNFWGSWCPP
                     CRSEMPEIQAAWEELDARGFKMIGIAMQEDPRDAVTYRDRVGATFPVAVDPNYLAAVA
                     DPSAFPDLFATAATWEIRNFPTHVFIDADGVVRAVVIQQMDTDTAIEYAESVMTDEQK
                     ASASAGD"
     gene            complement(8090..8992)
                     /gene="ccsB"
                     /locus_tag="VGR22_03360"
     CDS             complement(8090..8992)
                     /gene="ccsB"
                     /locus_tag="VGR22_03360"
                     /inference="COORDINATES: protein motif:HMM:TIGR03144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEV2127639.1"
                     /translation="METLVKLSFYFFAASSVALGAASLSYIAYTIGRVRVRRATMATS
                     AGTTVTSSSTDFSPGNTAFGRWGSMLTWFGAVFAFLAVLFRTIAAERLPTANMYEFSL
                     TFVLAISIIYIAFERLYDFRQLGAIVLPIAFGMTVYIWSLPAELREITPLIPALQARP
                     IMTAHVSAAIFAYATFAVSFAAAIFYLIKSKRNIRWLPSLEVLDDVAFRSVAIGFPLL
                     SLVLILGSIWAHDAWGVYWSWDPKETAALFTWLVYAVYLHTRTLRGWRGTRSAVILLF
                     GFGAVMFTYYGNYFFGGLHAYGGV"
     gene            complement(9058..10545)
                     /locus_tag="VGR22_03365"
     CDS             complement(9058..10545)
                     /locus_tag="VGR22_03365"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEV2127640.1"
                     /translation="MAQASATTRPPSRSPLETAVDRVWRFFCSVRAAIYEISFLALLV
                     LIGTLRGSEVPQWIADAAPPLQPLVDRWYAWDVFGSPLFAATLAVIAIAIAVCTLNRV
                     PGIWQTISEPRVRTSVGYVARSEVTAEFIGASNPEALEQRLRDALADRRYRVFTERVG
                     DQIHVYADKNRWSKFGTFPFHLALILLLVGGIVASMFGFRDTEFAVAEGETRAVGHGT
                     DLSVELVRFRDAYIETGDPAEYRSDVIIYDDGSKVKDGEITVNSPISAGVATFYQASF
                     GIASDIVVRDALGNVLFDAPVEMGFFNLRANPDAPAGLIRLPAQGVQIAVVAPDVDRA
                     NRPDLDTLNLANGQVWVQVSPLNSSPGSGNMETAVLDQGDPAEVGGYEVLFDRESRFT
                     VLQVAYNPGIPIFFIASVLLVGGLAVTFYFPMRRIRGIIQRSAEDSSLIMTPLAKRDW
                     GGKREFFRVSEEVGKQLGVTPKVKRPDHEGYWDDESDASEDDTNL"
     gene            complement(10612..>10916)
                     /locus_tag="VGR22_03370"
     CDS             complement(10612..>10916)
                     /locus_tag="VGR22_03370"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEV2127641.1"
                     /translation="VGPILGAILTMAAGQGSIGRATTLLPVYSLGLAVPFMLAALAFG
                     SAPGIIRKLNSRMAMVTTISGAVMIGVGAIMILGVYEQIFIELVRVAPWTPYEPEI"
BASE COUNT         1999 a         3395 c         3782 g         1740 t
ORIGIN      
        1 ccgttcgacc gagatgccga ggtcgaacgg cgggcaccgg tttgtcgcat cgcgctttga
       61 ccggcaccac ggacctgggc tcaaagggag tcccggggag gtgtggagca cacgagagcc
      121 gcgggacaga ccgaagacaa cacattggac ttggatcagg cgccgggcgg cgagtctggc
      181 ttgaaatggt gatcacccgc cgcgaaaccg ccctgatacg tagcgaggta ccacgtcgct
      241 tcacctgcgg catgtaccaa acaggtgcgc cgccgggcgg cgcctcagga gcagcaagac
      301 attgagtgag caggacaagg accgaaagac cgtgcccgag aaccagcagg gccagggacg
      361 aagccgccga cggcgtcgac gtcggccgca aggggcacag ggcggtaacc agcagcaaca
      421 gggccaggat cggacccagc agtctgccgg ggctgagtcg gatgaggcgc gggcgtcatc
      481 ggggcagcgg cggcgccttc gaggtggccg gggacgtggc ggccccagcc agcagcagga
      541 ccagcagggt ggcaaccagc aggcacggca gcagggaggc cggcgcggtg ggcaacaggt
      601 cgcgagcagt gagcagcagg cgcaggagaa ggggcagggg cagcgacgcc ggcgagccaa
      661 ccgtcgaccc cggcgagagg agcatgcccc ggaacgcgtc gagccgcggc gcgagccgca
      721 cgagcgggcg cgcggccggc aggccggtgg tggtcgcgga cagggccccg cgccgatcct
      781 cggcgagcgc atgcgcatca tcggcgcccc gaggaccggc gcggtacccg cgccgatcgg
      841 caaggaccgc ctgcgcgtga ttccccttgg gggcgtcggt gaggtcggca agaacatgac
      901 cgccgtcgag agcggtaagg atatcatcct cctcgacgcc ggcggcaagt tccccgagga
      961 ggatcagcag gggatcgacc tcatcattcc cgacgtgacc tacatcaagg agcgtcggaa
     1021 gaactttcgg ggcatcctcc tcacacacgg ccatgaggac cacatcggcg cgctgcccta
     1081 cgtgattccg cagctctacg agggcaaggg ctccccgaaa atccctatct acggcactcc
     1141 cctcgccatc ggtttcgtgg agcggaagct cctcgaggcg cgggtggatg acatggtgga
     1201 gctgaagacg gtggagtccg gcgagcgtgt gaaactcggc gagaacatca gcgcagagtt
     1261 catccacgtg acgcactcga tcccggatgc aacatcgatc gcggtccaca cgccggtcgg
     1321 cacgatcgtc gatacgggag atttcaagtt cgacccgacc ccagtgatgg gcgatccagc
     1381 ggacgagaag cggctcagga agctcggcga caacggcgtg ctggcgctct tctccgacac
     1441 ggtccgcgtg gagtcggaag gcagcacgcc gtcggagaag gtcgtgctgg aaacgatcga
     1501 cgacgtgatc ggtcacgctg ccgggcaggt catcatcgcg accttcgcgt ccaacgtcag
     1561 tcgcgtgctg atggcgctca aggcggcgga gaagcatggc cgcaaggtgg cggtcgccgg
     1621 gcgcagcatg gagcagaaca cccgcgtcgc gctcgatctg ggctaccttg acccgccgga
     1681 gggcctgctg gtgacgctcg acgaggtgat gcgcctgccg aagaacaagc gggtgattgt
     1741 ctccaccggc agccagggcg agccggccgc ggcgctggcc cgcatcgcgg ccggcgagca
     1801 cccgaagatt cgtgtcggcc gtggcgatct catcctggtc tcggcgactc cgattccagg
     1861 caatgaggat accgtttccc gaaccatcga caatctcttc cagcaggggg ccgaggtggt
     1921 ctacagcgcg atcgatcgcg gcgtgcatgt atcgggacac gcgggccagg acgagctgcg
     1981 acggatgctc gagttcatcc ggccgaccta cgcggtgccg gtacacggcg agtaccggca
     2041 tatggcgctc taccgggagc tggctgtcaa gagcggcgtg aagcgggaga acgtgctgct
     2101 gccggagatc ggcggggtga tcgagttcac caagagcagc gccaaccagc gcgcccgggt
     2161 gccatcgggc aacgtgctgg tcgacaagct gggagagcgg gacggcgtca acgccgtgct
     2221 gcgcacccgc gagaacctca cggaggaggg ggtcgtggtg gtgtcgctgg tggtgagtcg
     2281 gtcgaacggc gagctcatct ccgggccgga catcacgggg caggagctcg gcaaggagct
     2341 cagcaacggc gcgctgcgtg aggccgagcg ggagctgaag cgctcgctga cccgtcggcg
     2401 gaagggcgcg ccgcagtacg gcttcctcgc ccagcgggtc aaggagtcgg ttgcggagac
     2461 cctttaccga cgcacgcggc accggccgct tatcttgccg gtgatcgccg agctgtagag
     2521 gatgccgtcg acaacatgaa acggacgaac cgccagaagc ccgccgtcac tgggcttctg
     2581 gcggtttccg ttcgacacgc cggtggtcac acaaccttcg gttgatcatc gtgcgagcgc
     2641 ggccaccggc ggcaagcgcc agccctcggc atcgtcttgg cctcgcctac caagccggcc
     2701 cttcggcttc gctcaaggca ggctactggc cttcggccaa gtacggcccc tattggctga
     2761 tggtcctgcc accattcagc aggggctcgt cggcgcccta tcgtggcgcg ctgcctggcc
     2821 tccggcaggt aaaggctacg gaatcgctcc ggcggggggc gagatactgc cgaactcgtc
     2881 gcgcgcccgg gtgagcgggt cctcgttggc catgcccggg tcccaaggga ggggccggcg
     2941 tgtgtattgc tcacggtgga gccactgcgc gagctgtgac tccgggttca tgatctcgtc
     3001 cggatcgatc tgcgtccggt gagcgatgat cgcctgtcgc ttcgcaccca gccaccgaga
     3061 gacgtcgagc cagtgggtga tcgtcgaagc gggagagccg agattgttgc tcgccttctc
     3121 ggaaatctcg ccgagcggct gggcaggatc acaggcgatg gccaatataa gctcgcgggg
     3181 caccgaggca aagtagagtg cgttggcgca ccagggctcg tggagcgcag gcagccagga
     3241 ggggtctgcc gccagctcga ccgcgcgggt ggtcagcttg cccatcagca cgtggtccgg
     3301 gtggccgtag atgccctccg gaccgaaggt aatgatcgta tcggggcgca ggtcccggat
     3361 atgcccgacc agatgtgtca gcagtgcctc gatgggctgg ttgatgagcg cgaggggatg
     3421 ctcgttttcc gacgtgttct ccatgccgga gtcgcggaac ccgaggatgc gcagctcgcc
     3481 gagtcccaga tgcgacatcg cctcgcggag ctcctgctca cgcacggcag cgagcgtgtc
     3541 tcgggttgcc aggtgggagt cgcgaatctc gccgacatcg ccgcgcgttg cgcagacgta
     3601 ggcggtgcgg agacccgcgc cgttggccca cgccatcgtc ccggcggagc cgaatgattc
     3661 gtcgtccggg tgcgcgagga tccagagcag gtcgacaggc gggttgccca agcgttcatc
     3721 cggtatcggc atgacacatc cttcttcgtt tccacatgca cctcacgtcg gggcccagaa
     3781 gttcaggatc gcctgctcca gagccgcctg gcgctcctgg ccggcgcggt agacgggcgt
     3841 ggcttccgct accggcgtgt aggtggcggc gactgccggt gactcgggcc gcagcgaagg
     3901 caccgcaccg atcgcctcaa gggcagacac cgcaacccct cgggccgagc cctcagcttc
     3961 cgcgtccagt gccgagagcg gccgacccaa ggtgtcggcg acgatctgga gccacagcgg
     4021 cgacttcagc gcggccgcgc cgttgacgta gatttcgtgg tcttgctcca gcatgggcag
     4081 gagcgcctca tggatcgagg cgaatcggta cgcagttccc tccagcaccg ctcggtagag
     4141 atgaccggga cgcgtcgcca aggtgagccc ggcgaagacg ccggaggcat catcgttcca
     4201 gctgggcgag cgctcgccgg cgaagagtgg cagcacggtc agaccatggc tgtcgggcgc
     4261 catccggttc gcctcggcgg tgaggatgtc gaagttgccg cccgcgaggt agcgggcgag
     4321 ccacccggtc acgttgccac cgttgctgag ggcgccaccc gtcacgcgat ggctccggtc
     4381 gagtcgatag gaccagatcc gtgcgctcaa ctccatggtg ggggtggttg cgtcctcggt
     4441 gatgatgcgc atggccccgg aggtgccgat cgtgatggcg acccgatcct tgcccactgc
     4501 accggtgccg aggttggcgg ccgcaccgtc tccgatcgcc ggaaaccatg gcacctcggc
     4561 gagggcgggc cagcgtgatt gccacgcgcc ggtgagcagt ggcaagggct cgtcccgatc
     4621 gaccagcgcc gggagccggt cctcgtcgag gtcgaggacc tcaagcagct cgcaatgcca
     4681 gcttgacgct tcgacatcga gcaggccggt ccccgatgcc atcgaaacgg atgtctgcat
     4741 gcagcggtgc aggcggcgat ccacgtagtc cgcgaatgaa caccaggtgc gtgtgtccgc
     4801 aacaatggcg ggacgctcct ggcgaagcca gagcagcttg gcgggccagt atgagctgtg
     4861 aaagcggcag ccagtttcgc ggtgcaactg ccgggcatcg agcgactccc ggaggcgagg
     4921 cacctcatcc ccggaccgct tgtcacccca catgtagacg ggagaggtgg gcgagccagc
     4981 cgcatcgacg cccatcaggc tgtgccagaa ggaggtggtg gccacggcgc cgatatcgga
     5041 cgcatgttcc ccggcgaggt ccagcacctg gtcgatgcac tcgatcacca gctggaagag
     5101 atcgtgcgga tctgcctcgg cgccaccatc cgcggtcgta tgcaggcggt gtgggatctg
     5161 gtgctcgctc gtgtcaatct gcaggccgtg cgcgtcgaac atcaacgcgc ggacggatga
     5221 cgatccgatg tcgacggcga gcacgagtgg cggctgggcc gcatcccagg ttgtttccgc
     5281 gtggccgggc tcgaaagcga ggcggttctg ctgcacggtt cgaagctcct cggtagggtc
     5341 cgggcgatgg acgcgatgtg cgggcccatc cgtcttgccg taggatgatg ccagatgtgt
     5401 cgtgtgtcgc gctccttccg gagcagtgta ggacaggaga agtgaatgcg agcgtgtagc
     5461 acgtgagcac agtctcaccc atcagtgtca tgcccgtggc gggactgccc gaaatctcgc
     5521 cgcgtgaccc gctcggtcga atgattgccg agcggatcga ggaggagatc ggcgggctgc
     5581 aggccgggga catcgtcgtc gttacccaca agatcatcag caaggccgag gggcagctgg
     5641 tcgacctcag tacggtctcg ccctcgccgc tggccgtcca gcttgccgag cgacatggca
     5701 aggatgcccg gcaggtcgag atggtgctgc gagaatccgc caggatcgtg cgtatgtctg
     5761 gcggtcatat catctcggag acccatcacg gcctcatttg tgcgaatgcg ggagtggacg
     5821 cgtccaacac ggacgtcggt gtcgtctcac tgctgccgcg cgacccggac gcgtcggccg
     5881 cgcgattgcg cgagcacctt gtgcgagacg tgccggccga ggacagcgaa cggatcggcg
     5941 tgatcgttac tgactcgttc ggccggccgt ggcgcaacgg catcgtgaac gtcgccatcg
     6001 gcgtggctgg catctcgcca cttgccgact accgcgggca gttcgacgcg gctggtcatg
     6061 agctgcgttc gacggtgctc gcggttgctg acgaactggc tgccgcagcc gagctggtga
     6121 tgcacaagac gcaggcgatc ccggtggcgg tggtgcgcgg gtacagctgg cagatcgagg
     6181 gtcagcccgg aaccgggcag gacctcatca tgccggtcga gcgcgacctc ttccgctagg
     6241 gcggcagcgc gtccataccg aaaggatttc aatcatgact caaggcgttg tcggttacgc
     6301 cgcctccttc gagcagtttc acccgaacga gctgcttgcc tacagccagc aagcggagca
     6361 gcaagggttc ggtgccgtga tggcgtcgga ccacttccat ccgtgggtcc cgagccaggg
     6421 gcactccgcg tttgtgtgga cgtggcttgg atcactcggc acggcgacct ccatcccgaa
     6481 gttcggcact ggtgtgacgc ctccgggcta ccgctaccac ccggcggtgc tcgcgcaggc
     6541 cgcggcgacg gtcgaggcca tgttccccgg gcgcttcttc ctcggactcg gcgccggcga
     6601 ggcgctcaac gagcacatcg tcggcgagta ctggccggag gcaccgatcc ggctcgagcg
     6661 gctgatggag tcgatcgaga tcatcgagcg gctcttcgag ggcaaggtgg tcaagcacca
     6721 aggcaagcac ttccgggtcg agagcgcaaa gctttacacg ctgccggaga aggcgccgcc
     6781 gatctacgtg gcgacgtccg gcccgatcat ggcgaagcgg accggcaagc acaccgatgg
     6841 catcatcacc gtcggtgccg cggacgagaa gctcaagatg ttgatggatc gcttcgagaa
     6901 gggcgcgcag gaggcaggaa aggatccgaa atcgatgccg cgcatgctcc agatcaaggt
     6961 ctcctatgcc gacaccgacg aggaggcgat tgacagcgcc gtgcgagact ggccgaacgg
     7021 cgggatgagt ttcccgaagg ccgacgtccg caacccagat gacttcgagg cgatggccaa
     7081 gctggtccgg ccggagaact tcaagaaccg cgtgctgacc tcaagcgatc tcgacgcgca
     7141 cgtggagtac atccagcgct tcatcgatct cggcttcggc gagatctacg tccacaatgt
     7201 caatcgggat caggagaagt tcattgacgc ctacggcaag cacgttatcc cgaatctcaa
     7261 ctggaagtag cgccggaggg taacagcagg aaacagggcg gacattgagt ctgcccgtac
     7321 gggtcaactc catgtcgacc cttgctcaag agaacagcct tgtgcccagc tgtgcgtcta
     7381 tgagtgtcgt tctgctagtc gcccgctgac gcagacgcct tctgctcgtc ggtcatgacc
     7441 gattcggcgt actcgatcgc cgtgtcggtg tccatctgct gaatcaccac ggcgcggacg
     7501 acaccgtcgg cgtcgatgaa gacgtgggtc gggaagttcc ggatctccca ggtcgctgcg
     7561 gttgcgaaca ggtcggggaa cgctgaagga tcggccaccg ccgcgaggta gttcgggtcc
     7621 acggccaccg ggaatgtcgc gccgacgcgg tcgcggtagg tgactgcgtc gcgcggatcc
     7681 tcctgcatcg cgatcccgat catcttgaag ccgcgcgcgt ccagctcttc ccatgccgcc
     7741 tgaatctcgg gcatctcgga gcggcacggc ggacaccacg acccccagaa gttcagccag
     7801 accccttcgc cgcggaggtc ggagagccgg aagacctcgc catcagcgta gagggtcatg
     7861 aactcgggag ccggctcgcc caccttcggc agcagcgagc tgttcacgcc accgctaccg
     7921 agctcagatg ctccctgtga gcgtgccagc cagacgcctg aggcaatgat gagcagcgcg
     7981 accgccgcca cgatgccaac ctgctgggcg cggctcgggc cggtcgtgct ggcgttttca
     8041 gtggcgggtg ccgatgtgga ttgggcggac acggattcct gcacgggttc tagacgccgc
     8101 cgtatgcgtg gaggccgcca aagaagtagt tgccgtagta ggtgaacatg accgctccaa
     8161 acccgaacag caggatcacc gcgcttcgtg tccctcgcca gccacgcagg gtgcgggtgt
     8221 gcaggtacac ggcgtagacg agccaggtga agagggctgc cgtctccttc gggtcccagg
     8281 accagtagac gccccaagcg tcatgcgccc agatcgagcc gagaatcagc accagtgaga
     8341 gtagcggaaa gccgatggca accgatcgga aggcgacgtc gtcgagcact tccaggctcg
     8401 gcagccagcg gatgttgcgc ttgctcttga tgaggtagaa gattgccgcc gcgaaggaga
     8461 ccgcgaatgt agcgtaagcg aagatcgccg cggaaacatg tgccgtcatg atcgggcggg
     8521 cctgcagcgc cgggatcagc ggcgtgatct cgcgcagctc cgccggcagg ctccagatgt
     8581 agaccgtcat gccgaaggcg atcggcagca cgatggcacc gagctggcgg aagtcgtaca
     8641 ggcgctcgaa ggcgatgtag atgatcgaga tcgccagcac gaaggtgagc gagaactcgt
     8701 acatgttggc ggtcgggagc cgctccgcgg cgatggtgcg gaagagcacc gccaggaagg
     8761 cgaacaccgc accgaaccag gtcaacatgc tcccccaccg gccgaaggcg gtgttgcccg
     8821 ggctgaaatc ggtgctgctg ctggtcacag tggttccagc cgaggttgcc atggttgcgc
     8881 gccgaaccct gacgcggccg atggtgtatg cgatgtagct cagcgacgcg gctcccagcg
     8941 ctaccgagga cgctgcgaag aaatagaacg agagcttgac gagagtttcc atgatttctc
     9001 cgaatccgct acggagcggt ggccgccgat tatcgactgc tcgtcatgaa gcctatctta
     9061 caggttcgtg tcatcctccg atgcgtcgct ttcgtcgtcc cagtaacctt cgtggtcagg
     9121 gcgcttgacc ttcggtgtca ccccgagctg ctttccgacc tcctcagaga cgcggaaaaa
     9181 ctcccgcttg ccgccccagt cacgcttggc gagaggtgtc atgatgagcg agctatcctc
     9241 agcggaccgc tggatgatgc cgcggatgcg ccgcattggg aagtagaagg tgaccgccaa
     9301 gccaccgacg agcaagaccg aggcgatgaa gaagatagga atgccggggt tgtaggcaac
     9361 ctgcagcacc gtgaaccgcg attcgcgatc gaagagcact tcgtagccgc cgacttcggc
     9421 cggatcgccc tgatcgagca cggcggtctc catgttgccg gagcccggcg agctgttgag
     9481 cggcgagacc tgcacccaca cctgcccgtt ggcgaggttg agggtgtcga ggtccggtcg
     9541 gttggctcgg tcgacgtccg gcgcgacgac agcgatttgc acgccctgtg ccggcagccg
     9601 gataagaccg gccggggcat ccggattggc gcgaagattg aagaagccca tctcgaccgg
     9661 cgcgtcgaag agcacgttcc cgagcgcgtc cctgacgact atgtcgctcg caatgccgaa
     9721 gctcgcctgg tagaaggtgg cgacgccggc actgatcggg ctgttgacgg taatctcgcc
     9781 gtccttcacc ttgctgccgt cgtcgtagat gatgacgtcg ctgcggtact cggcgggatc
     9841 gcccgtctcg atgtaagcgt cgcggaagcg gacgagctcg acgctgaggt cggtgccgtg
     9901 gccgaccgcg cgggtctcgc cttcggcgac ggcgaactct gtgtcgcgga agccgaacat
     9961 cgatgcgacg atgccaccga cgagcagcag gatgagcgca aggtgaaagg ggaaggtgcc
    10021 gaacttcgac cagcggttct tgtcggcgta gacgtggatc tggtcaccga cgcgctccgt
    10081 gaagacgcgg tagcgcctgt ccgcgagggc gtcgcgaagc cgctgctcca gcgcctcggg
    10141 gttgctcgca ccgatgaact ccgcggtcac ctcagagcgg gcgacatagc ctactgaggt
    10201 ccgaacacgc ggctcactga tggtctgcca gatccccggc acgcggttga gcgtgcagac
    10261 cgcgatcgcg atcgcgatca ccgccagcgt ggcggcgaag agcgggctgc cgaaaacgtc
    10321 ccacgcgtac caccggtcca ccagcggctg cagcgggggc gcggcgtcgg cgatccactg
    10381 gggcacctcg ctgcctcgca aggtgccaat gagcacgagc agcgcgagga agctgatctc
    10441 gtagatggca gcgcgcaccg agcagaagaa ccgccaaacg cgatcgactg cggtctccag
    10501 aggcgagcgg gagggaggac gagttgtcgc cgatgcctgt gccatgaatg ctctcattca
    10561 attcgcggca gaaaccaacc gtgccgggcg tatgtgaaaa gaaagatatg atcagatctc
    10621 gggctcgtag ggtgtccacg gtgcgacgcg gacgagttcg atgaagatct gctcgtagac
    10681 accgagaatc ataatcgcgc cgacgccgat catcaccgcg ccgctgatgg tcgtcaccat
    10741 cgccatccgg ctgttgagct tccggatgat acccggtgcg ctgccgaagg cgagcgccgc
    10801 cagcatgaac ggcacggcga ggccaagcga gtagacgggc aggagcgtgg tcgcccgccc
    10861 gatgctgccc tgtcctgcgg ccatcgtcag gatcgccccg aggatcggtc cgacgc
//
LOCUS       DASYWL010000088         5323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_585929, whole genome shotgun sequence.
ACCESSION   DASYWL010000088 DASYWL010000000
VERSION     DASYWL010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5323
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585929"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VGR22_03375"
     CDS             <1..313
                     /locus_tag="VGR22_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV2127642.1"
                     /translation="FNAAWVAASLASLDRIVEEALAFAPTTGARPEPSATSEDKYGLT
                     ARERDVLRLIMEGLTDHEIAEALYISHRTVSTHVGHILAKLDVRTRTAAAAFAARDKL
                     V"
     gene            389..1675
                     /gene="kynU"
                     /locus_tag="VGR22_03380"
     CDS             389..1675
                     /gene="kynU"
                     /locus_tag="VGR22_03380"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HEV2127643.1"
                     /translation="MPTLPLTADYARALDDADPLESYRDRFYREPDSIYLDGNSLGLL
                     SRDAERSVLDALESWKEHGVEGWLAADPPWFTLGEALGRRMAPMMGAEPESVVVTGTT
                     TVNLHQLVGTFYRPDGKRHKIIATGLDFPSDVYALQSQVRLRGGDPNRDLVLVESHDG
                     RTIEEDDLIAAITDDVALVMLPSVLYRSGQLLDMRRLTRAAHDAGAIIGFDCAHSAGA
                     IPHELDAWGVDFAFWCSYKYFNAGPGAVGALYVNRRHFGTEPALAGWWGYHKERQFEM
                     AHHWEGAEQAGAWQISTIPLLSAAALLGSLAIFDEAGIEAVREKSLAQTDFLIALIEE
                     NGLLDTPYGYRIGTPREDSRRGGHVAVEHEHGAQIAQALRARGIVVDFRQPDVVRLAP
                     VALYTSYHDLWRTVQALLEIVDSGEHERAGESSLVT"
     gene            1976..3220
                     /locus_tag="VGR22_03385"
     CDS             1976..3220
                     /locus_tag="VGR22_03385"
                     /inference="COORDINATES: protein motif:HMM:NF020921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEV2127644.1"
                     /translation="MYRFDASISSTVRSDTAIVEEQPPLATASLDEQTLDRMHRYWQA
                     ANYLTVGQIYLQENPLLREPLKPEHIKPRLLGHWGTSPGLSLLYVHLNRLVRERRANM
                     IYLAGPGHGGPAVVANVYLEDTYTEVYSHITQDAAGMRKLFRQFSTPGGVPSHVSVPT
                     PGSIHEGGELGYVLSHAFGAAFDNPDLIVTAVVGDGEAETGPLSGAWQSISFLNPARD
                     GAVLPIVHLNGYKIANPTVLGRSSDADIHALLSGHGYAPFFVEGDDPMRIHQELAATL
                     DLCYGSIRAIQQDARENGVHERPRWPVIVLRTPKGWTGPEVVDEIPIEGTFRSHQVPL
                     SGVRTNPDHLRQLEEWLRSYNPDEQFDEEGRLAPELAKPGSRDSVCMMFSPSTRTGRG
                     PPRGQSTRLTAQWPWDCSAIFR"
     gene            complement(3232..4299)
                     /locus_tag="VGR22_03390"
     CDS             complement(3232..4299)
                     /locus_tag="VGR22_03390"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEV2127645.1"
                     /translation="MTIMLFILGVVLLVLLFVDVHMTVFVPRGGAGPIDRRIYRGGWW
                     MWRWLGDRFFSGTRRRWWLAQLGPVLMPLTILTWGVLLVAAFTLLYTPWVAGFTVSPA
                     KTGPMGDLAKLLYYSGYSAVTLGVGDVTPSATTPRILAFIEAGLGFALITAGISYLLS
                     VYNARNQSTTLSLAISHLVGRPDGQDPIDLLARTATIGSAGDLSAWMEQVSLELATLV
                     ELIGQYPLLRYFHEPNDDRAIPLAMRDLMELTTMSRTLLDRQAYPSLTQGPSMQAVHR
                     LGLHFLGEGAPGQSPEPEAVIAQRRGRYEEARSRLEKADVILRSDADAWHRFAEMTSA
                     WDLQDDGMRAALGYHTEADED"
     gene            4757..>5323
                     /locus_tag="VGR22_03395"
     CDS             4757..>5323
                     /locus_tag="VGR22_03395"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV2127646.1"
                     /translation="MTERSQQDPKEFGAKPPFPQEELEYPGLESNMTPRPDYGEESYT
                     GSGKLEGKVALITGGDSGIGRAVALAYAREGADVVISYLSEDSDAQETVRIVESTGRR
                     ALAIPGDIGEEQHCLNLIDRTVKEFGRLDILVNNAAFQSSYDIQELPSEDWDRAFKTN
                     IYATFWLSRAALRHLPEGGSIISTTSIQS"
BASE COUNT          969 a         1837 c         1673 g          844 t
ORIGIN      
        1 gttcaacgcg gcatgggtcg ccgccagcct cgcctcgttg gaccggatcg tcgaggaggc
       61 gttggcgttc gcccccacga cgggggcgcg ccccgagccc tccgccacca gtgaggacaa
      121 gtacggcctg accgcccgtg agcgagacgt cctgcgcctg atcatggagg gcctcacgga
      181 tcatgagatc gccgaggcgc tgtatatcag ccaccgcacc gtcagcacac acgtcggcca
      241 catcttggcc aagctcgacg tccgcacgcg caccgccgcc gcggccttcg ccgcacgcga
      301 caaactcgtc tgacgccgcg tttccccgca ttttctgcca cactgacagc attgccgccc
      361 gtcaggccgc cgaagacgag gatggtctat gccgacactc cccctcaccg ccgactacgc
      421 ccgtgcgctc gacgacgccg atccgctgga aagctaccgg gatcgcttct accgcgagcc
      481 tgactccatc tacctcgacg gcaattcact cgggctgctc tcccgtgatg cggagcgttc
      541 ggtcctggac gcgctggagt catggaagga gcacggcgtc gagggatggc tcgcggccga
      601 cccgccatgg ttcaccctcg gtgaggcgct cggccggcgc atggcgccga tgatgggcgc
      661 ggagccggag tcggtcgtcg tcaccggtac caccaccgtc aacctccacc agctcgtcgg
      721 caccttctac cggccggatg gcaaacgcca caagatcatc gcgaccggac tcgacttccc
      781 ctctgacgtc tacgccctcc agagccaggt ccgccttcgc ggcggcgacc cgaaccgaga
      841 cctggtgctc gtcgagagcc acgacggccg taccatcgag gaggacgacc tcatcgcggc
      901 gatcacggat gacgtcgcgc tggtcatgct gccgtcggtc ctctatcgct ccgggcagct
      961 gctcgacatg cggcgcctga cgagagcggc gcacgacgcc ggtgcgatca tcggcttcga
     1021 ctgcgcccac tcggccggcg cgattcccca cgagctggat gcgtggggcg tcgacttcgc
     1081 cttctggtgc tcgtacaagt acttcaacgc cgggccaggc gcggtcggtg cactctatgt
     1141 caaccggcgg cacttcggca ccgaaccggc actggccggg tggtgggggt accacaagga
     1201 gcgccagttc gagatggcgc accactggga gggggccgag caagccggtg cctggcagat
     1261 cagcaccatc ccgctgctct cggcggcggc actgcttggc tcgctggcga ttttcgatga
     1321 ggcgggcatc gaggcggtgc gagagaagtc gctcgcgcag accgacttcc tcatcgcgct
     1381 gatcgaggag aacggtctgc tggacacgcc gtacggctac cggatcggga cgccgcggga
     1441 agacagccgg cggggcgggc atgtcgccgt cgagcatgag cacggcgcac agatcgccca
     1501 ggcactccgg gcgcgcggaa tcgtcgtcga cttccggcaa ccggatgtcg tccgcctcgc
     1561 gccggtcgcc ctctacacca gctaccacga tctctggcgc acggttcagg cactgcttga
     1621 gattgtcgac agcggcgagc acgagcgcgc cggggagtcc agcctggtga catagcgttc
     1681 catccaccat cgatgatgcc ttccaacccg tgcagaatct cactgggtaa tgtcttggca
     1741 ggtgacagca tggctagcac ccgcatcggc gtacgacgca tacgtcagcg acggaacgcc
     1801 cgtttcgtga gaccgacata tcagcgaggc gaatgactgt tacagattcg tatcaaccgc
     1861 gtggcaatgg tccctacgca atggaccggc acgtgcgagc cgtgtgagca tcaccatgga
     1921 cgtgccatcg cgcgcgtcac cgttggtgat cggaactgca cctcagggag catgcatgta
     1981 ccgattcgat gccagtatct ccagcaccgt gcggtccgac accgctattg tcgaggagca
     2041 gcctcccctc gcgaccgcct ccctcgacga gcagacgctc gaccgaatgc accgctattg
     2101 gcaagcggcc aactacctca ctgtcgggca gatctacctg caggagaacc cgctgctgcg
     2161 cgagccgctg aagcctgagc acatcaagcc gcgattgctc gggcactggg gcacgtcacc
     2221 gggcctgagc ctcctctacg tccacctcaa ccgcctcgtc cgggagcgcc gggcgaacat
     2281 gatctatctt gccgggccgg gtcacggcgg cccggcagtg gtcgccaacg tctacctgga
     2341 ggacacctac accgaggtct actcccacat cactcaggat gccgccggta tgcgaaagct
     2401 gttccggcag ttctcgacgc ccggcggcgt gccgagccat gtcagcgtgc cgacgccagg
     2461 ctcgatccac gagggtggcg agctcggcta tgtgctgtct cacgccttcg gcgccgcgtt
     2521 cgacaacccc gacctgatcg tcacggccgt cgtcggcgac ggcgaggccg agaccggccc
     2581 gctgtcgggc gcgtggcagt cgatctcgtt tctcaacccg gcgcgcgacg gcgcggtgct
     2641 gccgatcgtc cacctcaacg gctacaagat cgccaacccg acagtgctcg gccgcagctc
     2701 ggacgccgat attcacgccc tgctcagcgg gcacggctac gcgcccttct tcgtcgaggg
     2761 cgatgatccg atgcggatcc atcaggagct cgccgccacc ctcgacctct gctacggcag
     2821 cattcgcgcg atccagcagg acgcccggga gaacggcgtg cacgagcgcc cgcgctggcc
     2881 ggtcatcgtg ctgcgcaccc cgaagggctg gaccggcccc gaggtggtcg acgagatccc
     2941 gatcgagggc accttccgct cccaccaagt gccgttgagc ggtgtccgga ccaaccccga
     3001 tcatctccgc cagctggagg agtggctgcg gagctacaac ccggacgagc agttcgacga
     3061 ggagggccgg ctcgcaccgg aactcgccaa gccagggagt cgagacagcg tatgtatgat
     3121 gttctcacct tcaacacgca caggtcgagg tccaccacga ggccaatcca cccggctgac
     3181 agcgcagtgg ccgtgggatt gctcggcaat ttttcggtga tgcggacgct ctcagtcctc
     3241 gtcggcctcc gtgtgatagc cgagagcggc gcgcatgccg tcatcctgca gatcccaggc
     3301 actcgtcatc tccgcgaagc gatgccaggc gtcggcgtcg gaccgcaaga taacgtccgc
     3361 cttctccagc cggctccgtg cctcctcgta gcgcccgcgc cgctgcgcta tgaccgcctc
     3421 gggttccggc gactgtcccg gtgcaccctc cccgaggaag tggagaccca ggcggtgcac
     3481 cgcctgcatg ctcgggcctt gggtaagcga gggatacgcc tgccggtcga gcagggtacg
     3541 gctcatcgtc gtgagctcca tcaggtcgcg catggcgagc gggatggcgc ggtcatcgtt
     3601 cggctcatga aagtagcgca gcagaggata ctgtccgatc agctcgacca gtgtcgccaa
     3661 ctccagcgag acctgctcca tccaagcgct gaggtcgccg gccgaaccga tcgtcgccgt
     3721 gcgcgcaagt agatcgatcg ggtcctgacc gtcaggtcgc ccgacgagat gactgatagc
     3781 cagtgagagg gtggtcgact gattccgcgc gttgtagacg gagagcagat agctgatgcc
     3841 ggcagtaatg agcgcaaacc cgagccccgc ctcgatgaac gccagtatcc gcggggtggt
     3901 ggcgctgggc gtgacatcgc cgacgccgag cgtcaccgcc gaatagccgc tgtagtacag
     3961 cagcttggcc aggtcgccca tcgggccggt cttcgccggt gacacggtga accccgcgac
     4021 ccatggcgtg tacaacagcg taaatgcggc gaccagcagc accccccagg tcaggatggt
     4081 cagcggcatc agtaccgggc cgagttgggc gagccaccac cggcgtcgag tcccggagaa
     4141 gaatcgatca ccgagccacc gccacatcca ccagccgccg cggtagatcc gccgatcgat
     4201 aggccccgcc ccgccgcgcg gcacgaagac ggtcatgtgg acatcgacga agaggagcac
     4261 caggagcacg acgccgagga tgaacagcat gatcgtcatg acgtcgccct cgtcaggcac
     4321 gcggtcttgc caggcatcat ctccctccct gtcccggtca tctcttgccg ctcaaagtgt
     4381 acgacggtgc gccgaacgca cagtcccggc gccagcacaa gcaggtcgac cacaagcacg
     4441 aacagcttgc cgttgcgaga ttttctccgg aggtgtccgg ctgacctgtg aaccaccatg
     4501 catgcgttga accccgtatc cacggcgatc ttcttgccac caagaagcgg tcaagcccca
     4561 ggacgggggc cggggtatcc gaccggtcgc atcgcaaggg ttatcagaga gcatggccac
     4621 gctacaggtt gtgccagtct cgctcggcac gaacgttcgg cgtggcaccc actgtgcgtt
     4681 agtgaggatc gggcagaccg aaccaaggga ccggcccgca tgcgccacag ccatcaccga
     4741 cggaggacct ttcccaatga ccgaacgcag ccagcaggat ccgaaggagt tcggtgccaa
     4801 gcccccgttc ccacaggagg agcttgagta tcccggcctc gaatccaaca tgaccccgcg
     4861 accggactac ggcgaggagt cctacacggg ctccggcaag ctcgaaggca aggtcgcgct
     4921 gatcaccggt ggcgacagcg gcatcggccg tgccgtggcg ctcgcctacg cacgcgaggg
     4981 cgccgacgtg gtgatctcct acctcagtga ggactccgac gcgcaggaga ccgtgcgcat
     5041 cgtcgaatcc accgggcgca gggcgctggc gatcccgggc gatatcggtg aggagcagca
     5101 ctgtctcaac ctcattgatc gcaccgtcaa ggagttcggc aggctcgaca tcctcgtcaa
     5161 caacgccgca ttccagtcca gctacgacat ccaggaactt cccagcgagg actgggaccg
     5221 cgcgttcaag accaacatct atgcgacgtt ctggctaagc cgcgccgcgc tgcggcactt
     5281 gccggagggc gggtcaatca tcagcacgac gtcgatccag tcg
//
LOCUS       DASYWL010000089         7978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_22347, whole genome shotgun sequence.
ACCESSION   DASYWL010000089 DASYWL010000000
VERSION     DASYWL010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7978
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22347"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(415..801)
                     /locus_tag="VGR22_03400"
     CDS             complement(415..801)
                     /locus_tag="VGR22_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127647.1"
                     /translation="MTAQRCLAPGCGRFVRAGAVFCDRHEAADVVPVIVDDAESTRPH
                     FRERLEQGEYAGLFDEHLSRVLTQAAVAVSERGLVDEIGALRVVLARLLMEEEDLTKL
                     ASNGSRALRSRQRGRNGRSAGMWHKG"
     gene            1066..1608
                     /locus_tag="VGR22_03405"
     CDS             1066..1608
                     /locus_tag="VGR22_03405"
                     /inference="COORDINATES: protein motif:HMM:NF023043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEV2127648.1"
                     /translation="MKRMKIFVLLLIAMSLSTAGTAAQGAATPDATPAADTVIYMPSV
                     EYIDEAGNVIAVMTVTDIEQDWTDYAEFYAPRAGNEFVRVTVQVESRVGRGTFTVEPY
                     FFYLQDMDGFMYQAVPIPTVEEEAAGFDPFASILDLAGGETGELHLTFEVLSGVDLQA
                     LYFSPDLYTRLITVAEFDEE"
     gene            complement(1700..2467)
                     /gene="ubiE"
                     /locus_tag="VGR22_03410"
     CDS             complement(1700..2467)
                     /gene="ubiE"
                     /locus_tag="VGR22_03410"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEV2127649.1"
                     /translation="MMVNQTQEQESSAQDADSPVRRGAIQSGDMVRQMFNRIVPRYDL
                     MNRVMTGGRDVAWRNIVAKQAATHGDHVLDIATGTGDLAFATYQAGAKRVVGADFSEE
                     MIEAARKKAPKHGPDVQFMIADALNLPFDAESFDAATVAFGLRNMSDYDAALREMYRV
                     LRPGGEAIILEMTPFKRPILGPPFRFYFGRVVPVVGGVLSGDITAYRYLPSSVDAFPP
                     AHELAEMMQEAGFADVRYQLLGFGTVAIHSGTRPAQG"
     gene            complement(2601..3524)
                     /locus_tag="VGR22_03415"
     CDS             complement(2601..3524)
                     /locus_tag="VGR22_03415"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: protein motif:HMM:NF004751.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEV2127650.1"
                     /translation="MSETIAQTTSTTETRLPAWKLWLLAARPKTLPAAVAPVVVGSAI
                     AIHEGRFHALAALMALLTALLLQIAANFANDALDFKRGADPVERMGPTRVTSAGLLSH
                     RAVLVATGIALALAALTGLYLVARGGWPLLLLGIAAIVCAVAYTGGPFPLGYLGLGEV
                     FVFLFFGLAAVTGTAYAQTLELTPLSLAASVPVGALAVAILVVNNLRDLPTDAATGKR
                     TVAVRIGPERTRAEYRVMLVIALASPVAFWLAGWLTWWWLLTLGSWPLAWWLWHEIER
                     QRSRALNLTLAATARMQLVYSVLLAAALVLS"
     gene            complement(3530..4513)
                     /locus_tag="VGR22_03420"
     CDS             complement(3530..4513)
                     /locus_tag="VGR22_03420"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEV2127651.1"
                     /translation="MRITVVGAGAMGTLMAARLSSAMASGTGGAVSERPERVILYGRP
                     SPHLEAIQRDGVRLTERDGTTHTIPVQVTTEPADVAGSELIIVLVKSWATAESVKPLR
                     SHLTRDAVVLTLQNGLGNAAQLRSALLHDGVRPHVWLGVTTQAALRTEPGCVTHSGEG
                     ITVIGRRSSEVNTRLQEIAATLTSAGIPTTAVDDIHRWVWRKLAVNAAINPVTALADV
                     PNCAVRKNHDLQNACRLLASEAAAVASARGLALDEQQVMDAVNDVAEATGDNRSSMLI
                     DLENGLRTEIDAINGAIVKEAHRRGIPAPANQLMVSLIRARASRQERQGRD"
     gene            complement(4637..7030)
                     /locus_tag="VGR22_03425"
     CDS             complement(4637..7030)
                     /locus_tag="VGR22_03425"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEV2127652.1"
                     /translation="MSDWAFAYDEFDPERERQREALFTLANGYMGTRAAVTHARADDV
                     HYPGTYLAGIYNRAQSEIEGQIVNLETMPNAPNWLQLTWRIDDGEWFDLREVTIDHYR
                     EELDIRRGVLTREFTVEDDARRRTQVRERRFVHIRHCHLAGLEMTIHPENWSGRLEVS
                     WAIDGRVENALVESDRQLNADHLRPVDSGACDDEVLWLKTETTESRIKIDVTTRTRLR
                     LNDARTRHEAELHSEPGFIGQATTLDVNEGDTITVEKVAAIFTSRDPAISESGHAARR
                     AIADAPDFEMLLQSHVEAWHLLWGRCDIAMEDGTNDAQMIVRLHLFHVLQTASVHTRY
                     IDTGLLARGLSGEAYHGHIFWDELMVLPYLNLHFPSISRTLLQYRFLRLGEARRRAKD
                     AGYAGAMYPWQSGSTGQETTPPFYPNPRTGGWMRDYTPLQRHIGAVIAYNAWHYVEVT
                     DDREYLITEGAEMLLEIARFWSSIATFNPEHERYEIHGVMGPDEFHSGYPWSDEPGLS
                     NNTYTNVMAVRTLRTALAALERLPAWRRDELRQTLDLGDNELERWRDIAHRMRIIFLE
                     SGELAQFEHYERLEELDWNTYREKYGDLQRLDYILDAEGDTPNRYRIAKQPDVLMLFY
                     VLAREELEHIMADLGYEFDDKIYRRTVKYYTKRSSRGSTLSRVVEAEIMAGLDGSASW
                     QCLQAALQSDVGDIQGGSTGEGIHLGAMGGTIAIIERRYTGLDASGDVLHFNPALPDE
                     LQRLSMQLVYREQWIDVELSQRRLKLTTDTEATQPVTVAMGARSQQLTPATPIDWPW"
     gene            complement(7027..>7978)
                     /locus_tag="VGR22_03430"
     CDS             complement(7027..>7978)
                     /locus_tag="VGR22_03430"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEV2127653.1"
                     /translation="ISLGSINIDFQMRTDRWPEAGETLLAGNFLMTTGGKAANVAVLG
                     RRLGGEVTLIGHVGNDPLADVVLDQLGEDGIGLAHVTRLPETPTAVSTIFVRPDGNKT
                     IILASNANLAWDEERDAETVREVIASAPDGSVLVTDLEIPIGIVRCAMEAAHDRSFPV
                     VLDPSPADQFADTLGVLTSVLVPSTSEAGTLTGVAVDSLNAAERAGRALVDRGTPVVC
                     QKLLNGGCLVVTRASSEYIPSIPVDVVDKTGAGDAFASGMGVGLLEGKAVAAAATMGV
                     AASSLAVTAYGSRESYPSRKRLDEMARRVTEAREERTRQT"
BASE COUNT         1276 a         2740 c         2522 g         1440 t
ORIGIN      
        1 gcggagccat cggggtcgtc ccggccccgc gtctgctgcc ggtcacgacc acgcagccgc
       61 cgcacctgta cgcgcagggc ctcggccacc tggtcggacc cgttccgaag gtccgtcatg
      121 gttcattcct tccgtgttgt gagaaagagc gacgattcgt ttatcaaaga ccaactaact
      181 gtatgtcatc ccgagcgaag tcgagggatt ccccggcgcg tagcgccagt ctcttgcttt
      241 cggatgacgg gtagcgaggg ctggttggga tctgagaaag aacatcggcc cgcccctgcc
      301 gcgtggcagg ggcgggggaa tcttcgctgc gctcagagtg acgatcttct tcggcgagtc
      361 tgctcgtccg tcacccctcg tcgagctccg ctagaatctg tgtcagcgcg ctcgtcagcc
      421 cttgtgccac atccccgctg atcgcccgtt gcgcccgcgc tgcctggacc gcaacgctcg
      481 cgacccgttc gaggcaagct tcgtcaggtc ctcttcctcc atcagcagcc gggccagcac
      541 tacccgcagc gcaccgatct cgtccaccag tccccgttcg ctcacggcca ccgcggcctg
      601 cgtcagcacc cggctcaggt gctcgtcgaa cagtccggcg tactctccct gttccagccg
      661 ctcccgaaag tggggtctcg tcgactccgc gtcatcaact atgacaggca cgacatcggc
      721 ggcctcgtgc cgatcacaga aaacggcacc ggcgcggacg aatcgaccgc accccggcgc
      781 caggcatcgc tgggcggtca ctttggcgga ctccttgtgg atggtggttt cagaacaaac
      841 gttctactct tacgaacgca ttggagacgg gaaaatgcgc cactgaaccg gtcgaattcg
      901 gcagaacaac tcgcagaggc tcgcacgaag gtgggagagc acgcgcacat gaagcgatcg
      961 tgctgctagg gtgaacgcac acgggaccta gaagagtcgg attgcgggcg tacttgtcgg
     1021 accagttgcc gacgccgccc gcgacgtgag aaaggatgtg tcgtgatgaa acggatgaag
     1081 atcttcgtgc tgctcctgat cgccatgagt ctctcgaccg ccggcaccgc agcgcagggg
     1141 gcggccacgc cggacgcaac cccggcagcc gacactgtga tctacatgcc gagtgtcgag
     1201 tacatcgacg aggcgggcaa cgtcatcgcc gtcatgaccg tcaccgacat cgagcaggac
     1261 tggaccgact acgccgagtt ctatgcgccg cgggcgggga acgagttcgt ccgggtgacg
     1321 gttcaggtcg agagcagggt cgggcgcggc acgttcacgg tcgagccata cttcttctat
     1381 ctccaggaca tggatggctt catgtatcag gccgtgccga tcccgacggt tgaggaagag
     1441 gcagccgggt tcgatccgtt tgccagcatc ctcgatctgg caggtggtga gaccggtgag
     1501 ttgcacctga cgttcgaggt gctcagcggc gtcgatctgc aggccctcta cttctccccg
     1561 gatctctaca cccgtctgat cactgtcgcg gagttcgacg aggagtaggt cagtcgcctg
     1621 acggtggtca ctgcgcggcc accgtgacgt tcccccgcaa cttggcatgt tgcgccgggg
     1681 cgatcttggt gccgttggtc tatccctgcg ccgggcgggt accgctgtgg atcgcaaccg
     1741 tcccgaaccc cagcagctgg taccgcacgt ccgcgaatcc ggcctcctgc atcatctccg
     1801 ccagttcatg cgccggcggg aaggcgtcca ccgagctcgg caggtaccgg taggctgtga
     1861 tatcgccgct cagcacgcca ccgaccaccg gcaccaccct gccgaagtag aaccggaacg
     1921 gcggtccgag gatgggccgt ttgaatgggg tcatctccag gatgatcgcc tctccgcccg
     1981 gccgcagcac ccggtacatc tcccgcagcg ccgcgtcgta gtcactcatg tttcgcaggc
     2041 cgaacgcgac cgtcgccgcg tcgaacgact cggcatcgaa gggcaggttg agcgcgtcgg
     2101 cgatcatgaa ctgcacgtcg ggaccgtgct tcggcgcctt cttccgggcc gcctcgatca
     2161 tctcctcgga gaagtcggca ccgaccacgc gcttggcgcc agcctgatat gttgcgaacg
     2221 ctaggtcgcc cgtgccggtc gcgatgtcca gcacgtgatc gccgtgcgta gctgcctgct
     2281 tcgccacgat gtttcgccat gcgacatccc gcccgccggt catcacccgg ttcatcaggt
     2341 cgtagcgggg cacgatgcgg ttgaacatct gccgcaccat gtcaccgctc tggatcgccc
     2401 cccgtcgcac cggtgagtcg gcgtcctgtg cgctcgattc ctgttcctgc gtctgattca
     2461 ccatcatgtt cgcgtccttg cgtctgggat gcgcgattcg cccgttcaat gtcataggag
     2521 tggattcatt cgcctccggg gctttcacgg ggcaagctct cgtgcccgcc tggcgatcgc
     2581 ccagaggccg caatcgtgcg tcagctgaga accagcgccg ccgccagcag cacactgtag
     2641 accagctgca tgcgcgccgt cgctgccagc gtgaggttca gtgcccggct tcgctggcgc
     2701 tcgatttcat gccagagcca ccacgcgagc ggccaactcc cgagcgtcag cagccaccac
     2761 caggtcagcc atccggccag ccagaaggcc accgggctgg ccagcgcgat caccagcatc
     2821 acacggtatt cggcccgcgt ccgctctggc ccgatccgca ccgccacagt ccgcttgccg
     2881 gtggccgcgt ccgtcggcag gtcccggaga ttgttgacca ccaggatcgc gacggccagt
     2941 gccccgaccg gcaccgatgc cgccagcgag agcggggtca gctccagcgt ctgggcgtag
     3001 gccgtgcccg tcactgcggc aagcccgaag aagaggaaga cgaagacctc tccgaggcca
     3061 agatacccaa gggggaacgg cccaccggtg tacgccaccg cgcagacgat ggcggcaatg
     3121 cccagcagca gcagcggcca cccgccgcgt gcgacaaggt agagccctgt cagcgccgcc
     3181 agggccagcg cgatgccggt tgcgaccagc accgcccggt gcgatagcag tccagcggag
     3241 gtcacacggg ttgggcccat gcgctccacg gggtccgcgc cgcgcttgaa gtcgagtgcg
     3301 tcgttggcga agttcgcagc gatctgcagc agcagggcgg tcaacagcgc catgagtgcc
     3361 gccagcgcat ggaacctgcc ctcgtggatg gcgatcgccg atccgaccac cactggtgcg
     3421 accgccgccg gaagcgtctt cgggcgtgcc gccagcagcc acagtttcca tgccggaagc
     3481 cgggtctcgg tcgtgctggt ggtttgcgcg atcgtctcgc tcattaccgt caatcccggc
     3541 cttggcgctc ctgccgcgac gcccgcgccc ggatcagcga taccatcagc tggttcgccg
     3601 gcgcggggat gccgcgccgg tgcgcctcct tgacgatcgc gccgttgatc gcgtcgatct
     3661 ccgtgcgcaa tccgttctcg aggtcgatga gcatcgacga gcggttgtct ccggttgcct
     3721 ccgcgacatc gttgacggcg tccatcacct gctgctcgtc cagcgccagc ccgcgcgccg
     3781 atgccaccgc tgccgcctcg ctcgccagca gccggcaggc gttctgcaga tcgtggtttt
     3841 tacgcacggc gcagttcggc acgtcggcca gcgccgtgac cgggttgatc gccgcgttga
     3901 ccgccagctt gcgccagacc cagcggtgga tgtcgtccac agcggtggtg gggatgcccg
     3961 cgctggtgag ggtggcggcg atctcctgca ggcgtgtgtt gacctcgctc gacctgcgcc
     4021 cgatcaccgt gatgccctcg cccgagtgtg tcacgcagcc gggctccgtg cgcagcgccg
     4081 cctgcgtcgt cacgccgagc cagacatggg gccgcacacc gtcgtggagc agggccgagc
     4141 ggagctgcgc agcgttcccg aggccgttct gcagcgtcag cacgaccgca tcgcgggtca
     4201 ggtgcgacct gagcggcttg accgactccg ccgtcgccca gctcttcacc agcacgatga
     4261 tcagctcact cccggcgaca tcggccggtt cggttgtcac ctgtaccggg atcgtgtggg
     4321 tggtgccgtc tcgctcggtg agccgcacgc cgtctcgctg gatcgcttca agatggggtg
     4381 agggccggcc gtagaggatc acgcgctccg gacgttccga gactgcaccg cccgtgccgg
     4441 acgccatcgc gctcgacaat cgcgcggcca tgagggtccc catggcgcct gctcccacca
     4501 ccgtgatacg caccgtcgct caccccttgt caccatcggt ccggcagcca ctgaagcgcc
     4561 gtccggattc agtcccggtc gttctgcggc tattgtcgca tgatccccaa gccggtcgag
     4621 gagcctccgt ggagtctcac cacggccagt cgatcggtgt tgccggcgtc agctgctggc
     4681 ttcgggcgcc catcgccacc gtgaccggct gcgtcgcttc cgtgtcggtg gtgagtttca
     4741 gacggcgctg cgacagctcg acgtcgatcc actgctcacg gtagaccagc tgcattgaga
     4801 gccgctgcag ctcgtcaggc agggcagggt tgaagtgaag gacatcgcca cttgcgtcga
     4861 gaccggtgta gcggcgctcg atgatcgcaa tcgtcccgcc catcgcacca aggtgaatgc
     4921 cctcgccggt cgaaccgccc tggatatcac cgacatcgct ctgcagcgcc gcctgcaggc
     4981 actgccacga cgccgacccg tcgagcccag ccatgatctc cgcctcgacc acccggctga
     5041 gggtcgaacc tctggagctc cgcttcgtgt agtacttgac cgtccgccga tagatcttgt
     5101 cgtcaaactc gtagccgaga tccgccatga tgtgctcgag ctcttcccgg gccagaacgt
     5161 agaagagcat cagcacatcg ggctgcttgg cgatgcggta ccggtttggg gtgtcgccct
     5221 ccgcgtcgag gatgtagtcc agccgctgca ggtctccgta cttctcccga taggtgttcc
     5281 agtccagctc ctcgaggcgc tcgtagtgct cgaattgcgc cagctcgccg ctctccagaa
     5341 agataatccg catccggtgc gcgatgtctc gccaccgctc cagttcgttg tcgccgagat
     5401 cgagcgtctg ccggagctcg tcgcgccgcc acgctggcag tcgctcgagg gcggcgagcg
     5461 cggtccgcag cgtccgcacg gccatgacgt tggtgtaggt gttgttggag aggccgggct
     5521 cgtccgacca tggatagccc gaatggaact cgtcgggccc catcaccccg tggatctcgt
     5581 accgctcatg ctctgggttg aacgttgcga tcgacgacca gaaccgcgcg atctccagca
     5641 gcatctcggc gccctccgtg atgagatact cccggtcgtc ggtgacctcg acatagtgcc
     5701 aggcgttgta ggcgatcacc gcgccgatgt ggcgctggag cggcgtgtaa tcccgcatcc
     5761 atcctcccgt tcgcgggttc gggtagaacg gcggcgtcgt ctcctggccg gtgctgccgc
     5821 tctgccatgg gtacatcgca ccggcgtagc cagcatcctt ggcccggcgg cgagcctccc
     5881 ccagccgaag gaaccgatac tgcagcaagg tccggctgat gctcgggaag tgcagattga
     5941 ggtacggcag caccatcagc tcgtcccaga agatgtgccc atggtacgcc tcgccgctca
     6001 gcccacgggc gagcaggcca gtatcgatgt accgggtgtg gaccgatgcc gtctgcagca
     6061 cgtggaagag gtgaagccgt acgatcatct gtgcgtcgtt ggtcccgtcc tccattgcga
     6121 tgtcgcagcg accccacagc aggtgccacg cctcgacgtg ggattgcagc agcatctcga
     6181 agtcgggcgc gtccgcgatc gcacgccgcg ccgcgtgacc gctctcggag atggccgggt
     6241 cgcgcgaggt gaagatggcg gcgaccttct ccaccgtgat ggtgtcgccc tcgttgacat
     6301 ccagggtcgt ggcctgcccg atgaagcccg gctcgctgtg cagctcggcc tcatgccgtg
     6361 ttcgcgcatc gttcagccgg agtcgcgtgc gggtggtgac gtcgatcttg atgcgcgact
     6421 cggtcgtttc ggtcttcagc cagagcacct cgtcgtcgca ggcgccgctg tcaaccggac
     6481 gcaggtgatc ggcattgagc tgccggtcgc tctccacgag cgcgttctcg acccgtccgt
     6541 cgatcgccca gctgacctcg agccggcccg accagttctc ggggtggatc gtcatctcga
     6601 gccctgccag gtggcagtgc cggatgtgca cgaaacggcg ctcgcgaacc tgtgttcggc
     6661 gcctggcatc atcctcgacc gtgaactccc gcgtgaggac accgcgccgg atgtccagtt
     6721 cctcgcggta atggtcgatc gtgacctcgc gcaggtcgaa ccactcgccg tcatcgatgc
     6781 gccaagtgag ctgtagccag ttcggggcat tcggcatcgt ttcaagattg actatctggc
     6841 cttcgatctc actctgggca cggttgtata tcccggcgag ataggtcccg gggtagtgca
     6901 catcatcggc ccgtgcgtgt gtcaccgcgg cgcgggtgcc catgtacccg ttggcgagcg
     6961 taaagagcgc ctcgcgctgg cgctccctct cagggtcgaa ctcatcatag gcgaaggccc
     7021 agtcactcat gtctgcctcg ttcgctcctc gcgcgcttcg gtgacgcgcc gtgccatctc
     7081 gtccaatctc tttcgtgatg gataggactc ccgcgagccg tatgccgtca ccgccaggct
     7141 tgaggcggca acccccatcg tcgctgccgc tgccacagcc ttgccctcca gcagacccac
     7201 gcccatacca ctggcgaagg catcgcccgc gccggtcttg tcgacgacat cgaccgggat
     7261 cgacggaata tactccgagc tagcacgggt cacgacaagg cacccgccat tgaggagctt
     7321 ctggcagacc accggcgtgc cacggtcgac cagggcccgg ccggcacgct cggcagcgtt
     7381 cagcgagtcg acggcaacgc ctgtcagcgt cccggcttcc gacgtactgg gcaccagcac
     7441 gctcgtgagc acgccgagcg tgtcggcgaa ctggtcggcg ggcgacgggt ccagaacgac
     7501 cggaaatgac cggtcatgcg cggcctccat tgcgcaccgg acgatcccga tcgggatctc
     7561 caggtccgtg acgagcaccg agccatccgg cgctgaagcg atcacctcgc ggacggtttc
     7621 ggcgtcccgc tcctcgtccc atgccaggtt cgcattggat gcaaggatga tcgtcttgtt
     7681 gccgtccggc cgcacgaaga tcgtcgacac cgctgtcgga gtctccggca gccgggtgac
     7741 atgcgcgagg ccgatcccgt cctcgccgag ctggtccagg acaacatcgg cgagcgggtc
     7801 gttgccaacg tgcccgatga gcgtgacttc cccgcccagc ctgcgcccca gcaccgccac
     7861 gttggccgcc ttgccgcccg tggtcatcag gaaattgccg gcgagcagcg tctcgccggc
     7921 ctccggccag cgatctgtgc gcatctggaa atcgatgttg atgctgccga gcgagatc
//
LOCUS       DASYWL010000090         2780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_172724, whole genome shotgun sequence.
ACCESSION   DASYWL010000090 DASYWL010000000
VERSION     DASYWL010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172724"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VGR22_03435"
     CDS             <1..451
                     /locus_tag="VGR22_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127654.1"
                     /translation="SLTDLYTNYLKGWAMGLEFAYWNPLAAAQIVVSQPEIGPALQET
                     FDEMSVGVESMWQNAQIFRGDFEEREGWGYHDLDSWQAYFDTIHELGQVETQINAEDV
                     CKNDYVAGANDFDVEQVKEDARTYELNETFAAIDMPDGAGFTKDELG"
     gene            612..992
                     /locus_tag="VGR22_03440"
     CDS             612..992
                     /locus_tag="VGR22_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127655.1"
                     /translation="MNKTFHHIGLPAPDQKTPLPGESWVESTRCWVTNPAHHPQHIEW
                     LRYAPDSPVDPTVRQEPHICYTVDDLDAAIAGKEIALHPFEPGDPPFGRTAFTMEDGI
                     AVEYIELYPGRTWFDDELPKGSNL"
     gene            992..1822
                     /locus_tag="VGR22_03445"
     CDS             992..1822
                     /locus_tag="VGR22_03445"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEV2127656.1"
                     /translation="MADRLAVSTNSYHSYSLEEALAGIAAAGFTSVELSSVPGWTEHV
                     SRDASDSELDQVRKQVDGAGLTALSMSGHSDLASDEGAVAFRKALHIASQLDIPYVTT
                     STGGHDDSSQGSIEDQRREFLERFAPLADEAADAGISICIETHGGLSSTGAKAAELVE
                     AISKPNVGINYDTANVIFYGDVRPEEDIRAAAKYVNHLHVKDKAGGDKEWNFPAIGTG
                     NVDFAPIFKTLADVGFTGPVSVELEFQGEPWPPLEQVNEAMKTSLAFIRQHLPESQLQ
                     "
     gene            2023..>2780
                     /locus_tag="VGR22_03450"
     CDS             2023..>2780
                     /locus_tag="VGR22_03450"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2127657.1"
                     /translation="MTALRIAVLGAGFMGSTHARAYGTMDGVEVATICATSARRAEPL
                     ASELGCEWTEDIERTLADDSIDAVDICLPTPQHREATEAALRAGKHVLLEKPLALTMA
                     DAEAIVRAAETSNRVVMVAHVLRFWPEYVELKRIVDSGELGTPMYVNAVRRQPALNWM
                     DAGPRGHLTGGSLHDMLIHDFDAANWVLGKPDDAFARGDFNPANEAVDHAQVLVIYGS
                     GTSATIEGGSMMPESYPFTSRFEVLCDNGAVEYH"
BASE COUNT          559 a          906 c          857 g          458 t
ORIGIN      
        1 ctccctgacc gacctctaca ccaactacct aaagggctgg gcgatgggct tggagtttgc
       61 ctactggaac ccgctcgcgg cggcgcagat cgtagtcagc cagccggaga tcggcccggc
      121 gctgcaggaa accttcgacg agatgtcggt cggcgtcgaa tcgatgtggc agaacgcgca
      181 gatcttccgt ggtgacttcg aggagcgcga gggctggggc taccacgatc tcgactcgtg
      241 gcaggcctac ttcgacacga tccacgagct cgggcaggtc gagacccaga tcaacgctga
      301 ggacgtctgc aagaacgact acgtcgccgg cgcgaacgac ttcgacgtcg agcaggtcaa
      361 ggaggacgct cgcacctacg aactgaacga gaccttcgcg gcgatcgaca tgccggatgg
      421 tgccgggttc accaaggacg agctggggta ggaaggcatc tcgtaggggc ggaccctaaa
      481 cggtgtccgc ccgttccatt ggctgtaccc aacctgctcg gtcgtcgcca cgctggaaag
      541 gacgataccc tcacggagtc actctcgcga ttgggtttcg gttcgctggc gaatacctgc
      601 ttggaggggc cgtgaacaag acgtttcacc atatcggctt gccggcaccg gaccaaaaga
      661 cgcccctgcc gggcgagtcg tgggtagaga gcacccgctg ctgggtgacc aacccggccc
      721 accacccaca gcacatcgaa tggctccggt acgcgcccga ctcaccggtc gacccgacag
      781 ttcggcagga accgcatatc tgctacaccg tcgacgacct cgacgcggcg atcgccggca
      841 aggagatcgc gctgcacccc ttcgaaccgg gcgaccctcc gttcgggcgc acggccttca
      901 ccatggagga tggcatcgcc gtcgagtaca tagagctcta tccgggtcgc acatggttcg
      961 atgacgaatt gccgaaagga tcaaacctgt aatggccgat cgactcgccg tttccaccaa
     1021 cagctaccac agctactcgc tcgaagaagc tctcgcaggc atcgccgctg ccggcttcac
     1081 cagcgtcgag ctcagttcgg tcccgggctg gaccgaacac gtcagccgtg acgccagtga
     1141 ctccgagctc gaccaagtcc gaaagcaggt cgatggtgcc ggtctcacgg cgctcagcat
     1201 gagcggccac tctgacctcg cctctgacga gggagccgtc gccttccgca aggccctaca
     1261 cattgccagc cagctcgaca tcccctacgt caccaccagc actgggggac acgacgactc
     1321 ttcccaagga tcgatcgagg accagcgccg cgagttcctc gagcgcttcg ccccactcgc
     1381 cgacgaggcg gccgatgcag gtatcagcat ctgcatcgaa acccacggcg ggctctcgag
     1441 caccggcgcc aaggcggccg agctggtcga ggcgatcagc aaacccaacg tcggcatcaa
     1501 ttacgacacc gcaaacgtga tcttctacgg cgacgtccgc ccggaggagg acatcagggc
     1561 cgctgcgaag tatgtcaacc acctccatgt caaggacaag gctgggggcg acaaggagtg
     1621 gaactttccg gcgattggca ccggcaacgt cgacttcgcg ccgatcttca aaacgctggc
     1681 ggacgtcggc ttcaccgggc cggtctcagt cgagctcgag ttccagggag agccgtggcc
     1741 gccattggag caggtcaacg aagcgatgaa gacctcgctg gcgttcatcc gccagcacct
     1801 gcccgaatct cagctccaat agagtatccg ggaccatcca gcgcgttaag ttgaaatgca
     1861 cgcgtacgcc gtgtggcgag atggaattca atgcggcgcc tgggctgggg ctccggttcg
     1921 tgcacgcggt tgagatttct ccattccaca gctggcacag ctttggtcga aatgacagca
     1981 aggttgtcca ctccacaggc attccagaat caggggaaaa acatgactgc attgcgcatc
     2041 gcggtgctgg gagccgggtt catgggcagc acccacgccc gcgcctacgg aacgatggac
     2101 ggtgtcgagg tcgcaacgat ctgcgccacg agcgccaggc gcgccgagcc tctcgccagc
     2161 gagctcggct gtgagtggac cgaagatatc gagcggaccc tcgccgacga ctccatcgac
     2221 gccgtcgaca tctgcctgcc aacgccccag caccgtgaag cgaccgaggc cgcgctgcgg
     2281 gccggcaagc acgttctgct ggagaagccg ctcgcgctga cgatggcgga cgccgaggcg
     2341 atcgtgcgag cggcggagac cagcaatcgg gtcgtgatgg ttgcccacgt cctgcggttc
     2401 tggccagagt atgtggagct caagcgcatc gtcgacagcg gcgagttagg cacgcccatg
     2461 tacgtcaatg ccgtgcgccg ccagccggcc ctcaactgga tggacgccgg tccgcgcggt
     2521 catctcacag gaggctcgct gcatgacatg ctgatccatg atttcgacgc cgccaactgg
     2581 gtgctcggca aaccggacga cgcgttcgca cgtggcgact tcaaccctgc caacgaagcc
     2641 gtcgatcatg cccaggtact cgtgatctac ggctcgggaa cgtcggcgac gatcgagggt
     2701 ggctcgatga tgcccgagag ctaccccttc acctcccgct ttgaggtgct ctgcgacaac
     2761 ggcgcggtcg agtaccattt
//
LOCUS       DASYWL010000091         7558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_473359, whole genome shotgun sequence.
ACCESSION   DASYWL010000091 DASYWL010000000
VERSION     DASYWL010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7558
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473359"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..1299
                     /locus_tag="VGR22_03455"
     CDS             40..1299
                     /locus_tag="VGR22_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127658.1"
                     /translation="MPVHNSTWGRVLGYLVLFATLFAPMLMTSAATAQDAGTQSGTAS
                     VEIHKRLCPTGYSDNNYFQDCHDTPQDGIVFELGSLRASTGADGSVSFVELLGGTYTI
                     TESIPGDAAESVVFCSYAEQLEQNASFTYVPGGISLDVPGGSDIVCDWYNIPYNLRGD
                     PGDAPDGEGSSITIHARLCPPGYTGNQPFEVCHDTPKTNRTFSLGNLSATTGADGNTL
                     FYQLNAGTYTVREAGQGEFLYYNVFCSLAEDVNQSVPVTATDNGVQIDVPAGTDIICD
                     WYNIPESGPGDGDDGDEPGTLTIHKAVCPPGYEGQQYFQDCHDTRAEGVAFDVLGPEG
                     LLLTDITTNAQGVLTIDNIVVGGSVDVLEQFPNDLNTAVFVVYCSDDASNRVPFDYVE
                     RDDAGGIEINVFRGDDVLCDWYDIPAA"
     gene            complement(1467..1877)
                     /locus_tag="VGR22_03460"
     CDS             complement(1467..1877)
                     /locus_tag="VGR22_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127659.1"
                     /translation="MAQISPEPLYDHDLAHRAIDFLHTGNRRLFPFTEKMDAVRLEAF
                     KRDLRDGLAEVLDSGSARKTSATGFVTNGGYRLRGIVHEWRQAGGGWPAGADPDALDT
                     VLTPLDDDDPEDNLELEAALVEDELNDWRGWNQG"
     gene            2137..3894
                     /locus_tag="VGR22_03465"
     CDS             2137..3894
                     /locus_tag="VGR22_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEV2127660.1"
                     /translation="MSSSYLARLMSATGLLLGLLAMLLTAQPAATQTGDSSIEVHNRI
                     CPEGYEGENFFEDCHDNVLGSGYEFTFTNVDTGETYQGATNANGDVGFANLSAGTYTI
                     TGGPPGEFTETFAYCAIGTEENPNREQVNVEYVTGGIQFYLPADTNVICDWYWIPIGQ
                     GPTPTATEAAEPTAESSIEVHARVCPEGYEGTDYFNACHDNPVEAGLPFTFVNQDTGA
                     TYEGTTNENGNVGFANLNQGTFTITGGAPGEFTERFVYCSSGVGETSDLSPIEVSSIQ
                     GGVQFFLPASTNVVCDWYIIPQGQQGQPTPTAAPETFDLPIYKLLCQNDPGQSALQDF
                     VTVGTVPAGCEQFAGVSVTVTDGDGNVIGSCVTEANAPCYVTVTVGTTIFATEDLATV
                     PEGYAPLQGETHEVAIEPATEASVLFTNVQTAPTATVVPTATVPPPTPTPAPEGRPLA
                     IVMGTCPPPGELGETVVELPDLIGPELGPDEDAEVILAETSTGTIDFTLQELMDSEYS
                     LVAYSDDGAMPVACTPLEGQINADGELVLGLQEVNESGDAGIVYLAPTVDGNQTQFSV
                     FFAEGLAEEDPEATPTPVG"
     gene            4121..6796
                     /locus_tag="VGR22_03470"
     CDS             4121..6796
                     /locus_tag="VGR22_03470"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEV2127661.1"
                     /translation="MSTPVQGVTPLVSRTLDNGLTVFLREDHGAPLTSFWVWYRVGSR
                     NEQPGRTGLSHWVEHMQFKGTPSLEKGAIFREVSRHGGTLNAMTSMDWTAYFETLPAD
                     AIDLSLKIESDRMRNSLFDAAETESERTVILNERQGAENRPTYHLAEEMMGAAFRAHP
                     YQHMVIGHEADLRAITRDDLYTHYQLYYTPRNAFISVVGDFQTDEMLKRIEQAFGAIP
                     AGEPIDPVIAQEPPQRAERRVTLRRPSPAAYMMMGYRMPAASHPDMPALLVADSILSG
                     AKPMGLGGGSAMGRSSRLYRALVATGMTRGASSGMGLHIDPYLWTFSATALPGVEPER
                     IEETFEEQLERLRSEPASEEEFLKARKQTRAQYIYSSETVTAQAFITGQMEIVDHAGR
                     VDTMEEELAAVTPEDVQRVARTWLKPDQRTVGWQLPKNGASAFAGAELPVDPSMPVET
                     VRPFEIERPWYLSDGVETGGSYGFFRTTLPNGMIVLAQPRPGDPAIDGSISIEAGQNA
                     THDMRPGIASLMADTLNRGTESRTFEEYNEATDSLGAMIHIGAGRRTIDVDFHSLSED
                     LPAVLKLASDVVRHPSFPEDELERVRQQAITNLREDEDDTGSVAGDAMRELLYPEDNP
                     SRLPLDGTVASIEQFTREDLVEYHAAHVSPGVTTVTIAGGVASIEEAVEHLERAFGDW
                     TLATPVTTPPVPVGAPKSLLRDDRTIPGKSQANLVMAYPTLPRGHDDYYALNVANVIL
                     GQLGLMGRLGAIVRDEQGLAYHVSSSLSPGIATSLWTARAGVDPGNVDRTMEGILSEV
                     RRLRSEPVSEAELADAKTYIVGSLPLSLESLGGVTSLLQTIERFGHGLDYLDRFPDII
                     KALTVEDLHRAAQQHLDPDRVAIGTAGPGTTEGAA"
     gene            6793..7455
                     /locus_tag="VGR22_03475"
     CDS             6793..7455
                     /locus_tag="VGR22_03475"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEV2127662.1"
                     /translation="MRLIAVNVAQPAPLVDDRTGRSVMSGIGKRPVVADVVLVGETNI
                     MGDGQGDLVAHGGVDKAVYSYPHDHIPAWMAEIGYGEGKDAPFGENLSIFGMTEDGIH
                     IGDRWQWGDAVLEIAQPRWPCFKLGIHSGHRDLPARLIASERSGWYSRVVQNGEAPVE
                     GKLALIHRDADAPTVRETFRAAKGQLPVEAALQIAARPALANCWRETIQKRYAATEPV
                     SG"
BASE COUNT         1406 a         2590 c         2331 g         1231 t
ORIGIN      
        1 gcacactacc gatacacacg gacccatgga ggaacggtaa tgccagttca caactcaaca
       61 tggggccgag tacttggcta cttggttctc ttcgcaaccc tgttcgcccc gatgctcatg
      121 acctccgcgg cgaccgcgca ggacgccggt acccaaagcg gcacggcctc ggtggaaatc
      181 cacaagcgac tctgccccac cggctacagc gacaacaact acttccagga ctgccacgac
      241 acgccccagg atggcatcgt cttcgagctc ggcagcctcc gcgccagcac gggcgctgac
      301 ggcagcgtga gcttcgtcga gctgctgggc ggcacctaca ccatcaccga gagcatccct
      361 ggcgatgcgg ccgagtcggt cgtcttctgc tcgtatgccg aacagctcga gcagaacgcg
      421 tcattcacct acgtccccgg cggcatctcc ctcgacgtgc cgggcggcag cgacatcgtc
      481 tgcgactggt acaacatccc ctacaacctc cgcggggatc cgggtgatgc cccggacggt
      541 gaaggatcat ccatcaccat ccacgcgcgg ctctgcccgc cgggctacac cggcaaccag
      601 cccttcgagg tctgccacga cacgccgaag accaaccgca ccttctcgct cggcaatctc
      661 tcggccacca ctggtgccga cggcaacacg ctgttctacc agctcaatgc gggaacctac
      721 accgtccgcg aggcgggcca gggcgagttc ctctactaca acgtcttctg ctcgctcgcg
      781 gaggacgtga accagagtgt gccggtgacg gcaaccgaca atggcgttca gatcgatgtc
      841 cccgctggca ccgacatcat ctgcgactgg tacaacatcc ccgagtcggg tcctggcgac
      901 ggcgacgacg gggacgagcc tggcacgctc acgatccaca aggccgtgtg cccgccgggg
      961 tacgagggtc agcagtactt ccaggactgc cacgacaccc gcgccgaggg cgtggccttc
     1021 gacgtcctcg ggcccgaggg gctcctgctg accgacatca ccaccaacgc gcagggcgtg
     1081 ctcacgatcg acaatatcgt cgtcggtggc agtgtggacg tgctggagca gttcccgaac
     1141 gacctcaaca ccgcggtgtt cgtcgtctac tgctcggatg acgccagcaa tcgcgtaccg
     1201 ttcgactacg tcgagcggga cgatgcgggc ggcattgaga tcaatgtctt ccggggtgac
     1261 gacgtcctct gcgactggta cgatatcccc gcggcatagg ctcgcaagcg tgatcgacgc
     1321 tcgtgcgtgc gagggggtag cgagaacgct accccctcca tctgatcgat taccttgccc
     1381 cggtcgtcat gcccggatcc agcaatacac gccgtcatta tgccgctgag tagtgctctg
     1441 ttcgcgtttc cctcccgccc tcgtgttcag ccttggttcc agccgcgcca gtcgttgagc
     1501 tcatcctcga cgagcgccgc ctccagttcc aagttgtcct ccgggtcgtc gtcatcgagc
     1561 ggggtcaata ccgtgtccag cgcgtccggg tccgcaccgg cgggccagcc gccgccggcc
     1621 tgacgccact cgtggacgat tccgcgaagc cgatacccac cgttggtgac aaaccccgtg
     1681 gcagacgtct ttcgggcgct gccgctgtcc agcacctcgg ccagaccatc gcgcaggtcc
     1741 cgcttgaacg cctcgaggcg gacggcgtcc atcttctccg tgaacgggaa gagccgccgg
     1801 ttccctgtgt ggaggaaatc gattgccctg tgtgcgagat cgtggtcgta gagcggctct
     1861 ggggatatct gggccatgca gcactcctca cggggtcaac aacaacgctt tggctcggcg
     1921 ccgtcactac gggccaggcg gcaaccgcac caaaacggtt actgccgttc attgtacccg
     1981 ggggcgtccc ccggccggag cacgttcgtg gaacccctct tgcgggccac gtgtgttagc
     2041 gccatcgcgc gacaatcgcg attgcccgtg gcgcattgac cctagcggca gccggaaacg
     2101 atccggctca acgcaccaac aaggaggtgt accgacatgt cgtcgtcata cttggcgcgt
     2161 ctcatgagcg ccacaggttt gctacttggg ttgctggcca tgctgctcac agcgcagcct
     2221 gccgcgaccc agactggtga cagctccatc gaggttcaca accgcatttg tcccgaaggg
     2281 tacgaaggcg agaatttctt cgaggactgt cacgacaacg tgcttggttc gggctatgag
     2341 ttcacattca cgaacgtgga tacgggcgag acgtatcagg gtgcgacgaa cgcgaacggc
     2401 gacgtgggct tcgccaacct gagcgcgggc acgtacacga tcacgggggg gcctccgggc
     2461 gagttcaccg agacctttgc ctactgtgcc atcggcacgg aggaaaaccc aaaccgcgag
     2521 caggtaaacg tggaatacgt gaccggcggc atccagttct acctgccggc cgacaccaac
     2581 gtaatctgcg actggtactg gatcccgatc gggcaggggc cgacaccaac ggcaactgaa
     2641 gcggcggagc cgaccgccga gagctcaatc gaagtgcatg cccgcgtgtg cccggagggc
     2701 tacgagggca cggactactt caatgcttgc cacgacaacc ccgtcgaggc tggcctgccg
     2761 ttcaccttcg tgaaccagga taccggcgcg acgtatgagg gcacgaccaa cgagaacggc
     2821 aacgtcgggt ttgccaacct gaatcagggt accttcacca tcaccggcgg cgccccgggc
     2881 gagttcaccg agcgcttcgt ctactgctca tctggtgtgg gcgaaaccag cgacctgtca
     2941 cctatcgagg tcagctccat tcagggcggc gtacagttct tcctgcccgc cagcaccaac
     3001 gtggtctgcg actggtacat cattccacag ggccagcagg gtcagccgac accgaccgcg
     3061 gcgcccgaga ccttcgacct cccgatttac aagctgctgt gccagaacga tcccggtcag
     3121 tcggcacttc aggacttcgt gacggtcggt acggttcctg ccggttgcga gcagttcgct
     3181 ggcgtctcgg tcaccgtgac tgacggggac ggcaacgtga tcggcagctg cgtcaccgag
     3241 gccaatgccc cctgttacgt caccgtgacg gttggcacca cgatcttcgc cacggaggac
     3301 ctggcgacgg tcccggaggg atacgcacca cttcagggtg agacccatga ggtcgcgatc
     3361 gagccggcga ccgaggcgtc agtcctgttc accaacgtcc agacggcacc gacagcgact
     3421 gtcgtgccga cggcgactgt cccgcctccc accccgacac cggcaccgga gggccgcccg
     3481 ctcgccatcg tcatgggcac ctgcccgccg ccgggcgaac tgggtgagac cgtggtcgag
     3541 ctgcccgacc tcattgggcc ggagctcggt ccagatgagg acgccgaggt aattctcgcc
     3601 gagacgtcca ccggcacgat cgacttcacg cttcaagagt tgatggacag cgagtactcg
     3661 ctggttgcct actcggatga cggcgcgatg ccggtcgcct gcacccctct cgagggccag
     3721 atcaatgccg acggcgagtt ggtgcttggc ctgcaggagg ttaacgaatc gggcgacgcc
     3781 ggtatcgttt acctggcacc gaccgtggac gggaaccaga cgcagttctc cgtcttcttc
     3841 gccgagggcc tggcagagga ggatcctgaa gcgacaccga cgccggttgg gtaaaccagc
     3901 caaccgagag atcggcacgg aggggtggtc ccggtcggga ccgcccctcc ccttgtctgt
     3961 cctgagctgc cgtcgccgcc ggcatgtgca tcgcccaagg cgcgagtggc tctgtcgagt
     4021 atcctcacca gcagggatta ccgatcgtcg gctgcccgct gacggtcatc gtcgcgccgg
     4081 ttcgcccggc gcccacagca acggaaagca gaagccgcgc atgagtactc ctgtgcaagg
     4141 ggtcaccccg ctggtgtccc gcaccctcga caacggcctg accgtcttcc ttcgcgagga
     4201 tcacggcgcg ccgctcacct cgttctgggt ctggtatcgc gtcggcagcc ggaatgagca
     4261 gccgggccgg accggcctgt cacactgggt cgagcacatg caattcaagg gcacgccgtc
     4321 gctcgagaaa ggcgcgatct tccgggaggt ctcgcgccac gggggcaccc tcaacgcgat
     4381 gacctcgatg gactggacgg cctacttcga gacgcttccc gccgatgcga tcgatctctc
     4441 cctgaagatc gaatccgacc gaatgcgcaa ctcactcttc gatgccgctg agacggagag
     4501 cgagcgcacc gttatcctca acgagcggca gggcgccgag aaccggccga cctaccacct
     4561 cgccgaggag atgatgggtg ccgccttccg tgcccacccg tatcagcaca tggtcatcgg
     4621 ccacgaggcc gatctccgtg ccatcacacg tgacgacctc tacacccact accagctcta
     4681 ctacacgccg cgaaatgcct tcatttccgt cgtcggcgac ttccagaccg acgagatgct
     4741 caagcgcatc gagcaagcgt tcggtgccat cccggccggc gagccgatcg atccggtgat
     4801 cgcccaggaa ccaccgcagc gggccgagcg ccgcgtgacc ctgagacgcc cgagtcccgc
     4861 cgcctacatg atgatgggct atcgcatgcc cgccgccagc cacccggaca tgcccgcgct
     4921 gctggtcgcc gattcgatcc tctctggggc gaagccgatg ggacttgggg gcggcagcgc
     4981 catggggcga tcgtcgcggc tctaccgggc actcgtcgcc accggcatga cccgcggcgc
     5041 atcttccggt atgggcttgc acatcgaccc gtacctgtgg accttctccg ccaccgcgct
     5101 gccgggcgtc gagcccgagc gcatcgagga aacgtttgag gaacagctgg aacgtctccg
     5161 cagcgagccg gcctccgagg aggagttcct caaggcgcgc aagcagaccc gcgcgcaata
     5221 catctactcc agtgagaccg tcacagcaca ggcgttcatc accggccaga tggagatcgt
     5281 cgaccatgcc gggcgtgtcg acacgatgga ggaggagctt gctgcggtca ctccggagga
     5341 cgtgcagcgc gtcgcgagga cctggctcaa gccggatcag cgcactgtcg gctggcagct
     5401 gccgaaaaac ggtgccagcg ccttcgccgg tgccgagctt ccggtcgacc ccagcatgcc
     5461 ggtcgaaacc gtgcggccct tcgagatcga gcggccctgg tacctgagcg atggggtcga
     5521 gaccggcggc tcctacggct ttttccgcac gacgctgccc aatggaatga tcgtgctggc
     5581 gcaaccacgg ccgggcgacc cagcgatcga cggatcgatc agcatcgaag ccgggcaaaa
     5641 tgccacccat gacatgcgac ctggcatcgc ctccctgatg gccgacaccc tcaaccgcgg
     5701 aaccgagagc cggaccttcg aggagtacaa cgaggcgacc gacagcctcg gcgcgatgat
     5761 ccatatcggg gcaggacgcc gtacgatcga cgtcgatttc cattccctct ccgaggatct
     5821 cccagccgtg ctgaagctcg caagcgacgt cgtccggcac cccagctttc cagaggacga
     5881 gctggagagg gtacgccagc aggcaatcac caacctgcga gaggacgagg acgacaccgg
     5941 ctcggtcgcc ggggacgcca tgcgcgagct gctctacccg gaggacaacc cttcccgact
     6001 gccgctcgac ggcaccgtag catccatcga gcagttcacc cgcgaggacc tcgtcgagta
     6061 ccacgccgcg cacgtctccc ctggtgtcac caccgtgaca atcgccggcg gcgtcgcatc
     6121 gatcgaagag gccgtcgaac acctcgagag agcattcggg gactggacgc tggctacgcc
     6181 cgtaaccacg ccgcccgtcc cggtgggcgc gccgaaatcc ctgctccgcg acgaccgcac
     6241 catccccggc aagagccagg cgaacctggt gatggcctac ccgaccttgc cgcgcggtca
     6301 cgacgactac tacgcgctca acgtcgccaa cgtgatcctc ggccagctcg ggctgatggg
     6361 ccgcctcggc gcaatcgtcc gtgacgagca ggggcttgcc taccacgtat cgagctcgct
     6421 ctcgcccgga atcgccacca gcctctggac cgcacgcgcc ggcgtcgatc ccggcaacgt
     6481 cgatcgcacc atggagggta tcctgagcga ggttcggcgc ctgcgatccg agccggtcag
     6541 cgaggcggag ctggcggacg cgaaaaccta catcgtcggc agcctcccgc tcagcctgga
     6601 gtcgctcggc ggcgtcactt cgctgcttca gacgatcgag cgcttcggcc acggcctcga
     6661 ctacctcgat cgcttcccgg acatcatcaa ggcgctgacg gtcgaggatc tgcatcgtgc
     6721 ggcgcagcaa cacctcgatc ctgaccgtgt ggcgatcgga actgccggcc ccggcacgac
     6781 ggagggtgca gcgtgagact gatcgccgtc aacgtcgccc aaccggctcc actggtcgac
     6841 gaccgcaccg gccgctccgt gatgagcggc atcgggaagc gcccggtcgt cgctgacgtc
     6901 gtgctcgtcg gcgaaaccaa tatcatgggc gatggtcagg gcgacctcgt tgcacacggc
     6961 ggcgtcgaca aggcggtgta cagctacccg cacgaccaca ttcctgcatg gatggcggag
     7021 atcgggtacg gcgagggcaa ggacgctccc ttcggcgaga atctctcgat cttcgggatg
     7081 accgaagacg gcatccatat cggcgaccgt tggcagtggg gcgacgctgt gctggagatc
     7141 gcccagccgc gctggccctg cttcaagctc ggcatccaca gtgggcaccg cgacctgccg
     7201 gctcgactca tcgccagcga gagatccgga tggtactcgc gcgtcgtaca gaatggtgag
     7261 gccccggtgg agggcaagct cgcgctgatc caccgggacg cggatgctcc gacggttcgc
     7321 gaaaccttcc gggctgccaa gggccagcta ccagtggaag ccgcgctgca gatcgccgcc
     7381 aggcctgcgc tcgcgaactg ctggcgggag acgattcaaa agcgatacgc agctacggag
     7441 cctgtcagcg ggtagacaca ccggtcaccc atcgctccaa gggacatcca tcgctgcatc
     7501 aggcaattcg tggaagcaaa ggcgatggac agactgaaca ctgtacccgg gaaaggcc
//
LOCUS       DASYWL010000092         3588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_417126, whole genome shotgun sequence.
ACCESSION   DASYWL010000092 DASYWL010000000
VERSION     DASYWL010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417126"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..418
                     /locus_tag="VGR22_03480"
     CDS             98..418
                     /locus_tag="VGR22_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127663.1"
                     /translation="MLPYLALAFMVALLTRSSAAGISVATAILFLEGQILSLVAAAGG
                     VLERLPELFLSRNVEALMSVNTDGGRANMPDVWQAATVLLLYTVAFLAVAFWQFRRRD
                     VTVG"
     gene            623..1102
                     /locus_tag="VGR22_03485"
     CDS             623..1102
                     /locus_tag="VGR22_03485"
                     /inference="COORDINATES: protein motif:HMM:NF042511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6544 family protein"
                     /protein_id="HEV2127664.1"
                     /translation="MAALPEPAQRWLRWSCVVGKPIPTTIRLRQEGELRVGAFGWLDF
                     TAEEYYSTEPPAFIWTAKVGMAPGVSVIGQDRYVDGRGSLEMRLLGAISVASDDGPEM
                     DEGDLLRYLNEVMWFPAAALSPYITWDAIDAISARATMEYAGVSGTSGSLVTRRRSR"
     gene            1227..1907
                     /locus_tag="VGR22_03490"
     CDS             1227..1907
                     /locus_tag="VGR22_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127665.1"
                     /translation="MRAGASASPSDEETALFLVIPARRTNRKPFQDRALPDGLVNDLR
                     RAAEREGAWMWPVDGEHRVAVADLVDEGDRIQWADRRFRSELAHWLRPARRGDGLSSM
                     YMYGLGPLVVRWLDLGKSTGRKNRELAMSAPLLMVLGTGGDGMRDWLVAGQALARVLL
                     RARIDGVDASYLNQPVQVSRLRPWLADLVRVQGWPQVLLCLGYGTEVPASSRRPVDIV
                     LEEAGARR"
     gene            complement(2089..>3588)
                     /locus_tag="VGR22_03495"
     CDS             complement(2089..>3588)
                     /locus_tag="VGR22_03495"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEV2127666.1"
                     /translation="LALIHEIGKRQAMTPISTDQPRTDRNIVLEARASRRDLIIGASA
                     MAAGAALGLGGSSAAQETGLPQGTALVVSPRLPLWDVTAASASAILDGSATNWLQVGS
                     PLPHPIRRVGVDGIATNGMPTEASVADYEALVTELASNPGTIAVAPLPVLDSRVTALR
                     IDGTDPFLALATEAEPVFHVGIAGDMIPGRNVNLHMRGYGDFVFPWRRVAPLLSSFDF
                     TFGNFECVISDTIPFPEDTNANALDFVMGSAGIDGLHLAGFDAVSLANNHSIYNGSGW
                     GFDGFYDTINNLAAAGMPWFGAGEDLDQARAPHVGEVAGKTIALIGIDGVTANVDYPG
                     TWMVTSEAGATASKPGTNPLVEEQILADISNLAAQYDIVIPFFHMGEQYRWMLSEWAI
                     ELSRRCVDAGATTVVTSHPHVTQGMEWYDGTPLFYGIGNFIYDQMFTVDTSQGHIVDL
                     TFRGSEIVGFRLHGVAIEDFTQPRLLGPNEQAAFMDRIWRSNDFIRARV"
BASE COUNT          566 a         1112 c         1263 g          647 t
ORIGIN      
        1 cagtgtactg gtgatcgcgg cggagggctt ggatcgtacc ctgggcgacg gcttcgtgat
       61 cgacctgctg ctcggcatcg gatgaagctg gttcgcgatg ctgccatacc tggcgctggc
      121 gttcatggtc gcgctgctga cccggtcgag cgcggcgggc atcagtgtcg ccaccgcgat
      181 cctcttcctc gaggggcaga tcctctcgct ggtcgccgcc gccggtggtg tgctggagcg
      241 gcttcccgaa ctcttcctta gcaggaacgt cgaagcgctg atgtccgtca acaccgatgg
      301 tggtcgggcg aacatgccgg acgtctggca ggccgcaacc gtattgctgc tctacaccgt
      361 tgcgttcctg gcggtcgcgt tctggcagtt ccgtcggcgt gacgtgaccg tggggtaggg
      421 cctgcagcgt cgccatcgac gctggaaaag gacatgagca agccatgggc aaacgagcaa
      481 aaatcgtaat cggaagtgtt ggcgcggtgg cgattgtggc ggcatcgtcg atcgggattg
      541 tccggctggg gttcgagcga catatcgctg gagatatcga tgatctgctg gcaaccggag
      601 ccgcggccga gtcgctgagg atgtggccgc gctgcccgag cccgcgcagc ggtggctccg
      661 ctggtcctgc gtggtcggca agccgatccc gaccacgatc cggcttcggc aggaggggga
      721 gttgcgggtc ggagccttcg gctggctcga cttcacggcg gaggagtact actcgacgga
      781 gccgccggcg ttcatatgga ccgcgaaggt cggcatggcg ccgggtgttt cggtgatcgg
      841 gcaggaccga tacgtcgacg gccggggctc gctggagatg cggctgctcg gtgccatctc
      901 agtcgctagc gacgatggac cggagatgga cgagggggac ctgctgcgct acctcaacga
      961 ggtcatgtgg ttcccggcgg cagccttgag cccttacatc acttgggacg caatcgatgc
     1021 catctcggcg cgagcaacca tggagtatgc cggggtgagc ggtacatcgg gctcgctcgt
     1081 tacccgtcgc cgatcccgat gaccgcgagc tgacgatcgc ctgcggcgct gctctcttcc
     1141 acctgcggat cgccctgcgc catgccggca tcaccccgga agtcgagctc ctgcccgact
     1201 cgcgtgatcc cgacctgctg gcacgcgtgc gcgccggcgc ctcagcttcg ccttcggacg
     1261 aggagacggc gctcttcctg gtgatcccgg cgcgacgcac caaccggaag ccctttcagg
     1321 atcgagcgct ccctgatggt ctcgtcaatg atctccggag ggcagctgag cgcgaaggcg
     1381 cctggatgtg gccggtcgat ggcgagcacc gggtcgccgt tgccgatctc gtcgatgagg
     1441 gtgatcgcat ccagtgggct gaccggcgct tccgcagcga gttggcgcac tggctgcggc
     1501 cggcccggcg tggtgacggc ctctcctcga tgtacatgta tggacttggc cccctggtcg
     1561 tccggtggct cgatctcggg aagagcaccg gccgcaaaaa tcgcgagttg gccatgtccg
     1621 cgccgctgct gatggtcctc ggcaccggtg gggacggcat gcgggactgg ctcgtggcag
     1681 gacaggcgct cgcgcgggtg ctgctgcgtg cccgaatcga cggcgtggac gcatcctact
     1741 tgaaccaacc ggtgcaggtc tcccggttgc ggccttggct ggcggacctg gttagggtcc
     1801 aaggctggcc gcaggttctg ctgtgtctcg gctacggcac cgaggttcca gcatcatccc
     1861 ggcgcccggt cgacattgtt cttgaggaag ctggcgcccg ccgatgaggc gagacgtacc
     1921 gagcaaggcg tgagctcggt tcagtgcagg cgggtcgccg tggtcgcgct cgatcgtgcc
     1981 tggcatgtaa gggatctcgg cgggccggaa cgccaagtgg tgcatggctt gcccggagtc
     2041 acgccatcat cccatggcga cgtcccaagg cagggcggtg gtgttcgact aaacccgggc
     2101 gcggatgaaa tcgttcgagc gccagatacg gtccatgaag gctgcctgct cgttcgggcc
     2161 gagcagccgc ggctgcgtga agtcctcaat cgccactccg tgcagccgga acccgacgat
     2221 ctccgaccct cggaacgtca ggtcgacgat gtgaccctgc gaggtgtcca ctgtgaacat
     2281 ctggtcatag atgaagttgc cgatgccgta gaagagtggg gtgccgtcgt accactccat
     2341 cccctgcgtg acgtgcgggt ggctggtcac caccgtcgtc gcacccgcat cgacacagcg
     2401 gcgggagagc tcgatcgccc actcggagag catccagcgg tactgctcgc ccatatggaa
     2461 gaaggggatc acgatgtcgt actgcgccgc caggttggag atgtcggcga ggatctgctc
     2521 ctcgacgagc gggttggtgc ccggcttgct cgccgtcgcg cccgcctccg acgtcaccat
     2581 ccacgtacca gggtagtcga cgttcgccgt gaccccgtcg atgccgatca gcgcgatcgt
     2641 cttgccggcg acctcaccga catgcggagc gcgagcctgg tcgaggtcct caccggcgcc
     2701 gaaccagggc atccccgccg cggcgaggtt gttaatggtg tcgtagaagc cgtcgaaccc
     2761 ccagccgctg ccgttgtaga tcgagtggtt gttcgccagc gagacagcat cgaagccggc
     2821 gagatgcagc ccatcgatcc cggccgagcc catcacgaag tcgagggcgt tcgcgttcgt
     2881 gtcctcgggg aaggggatcg tatcggagat gacgcactcg aagttgccga aggtgaagtc
     2941 gaagctgctc aacaggggag ccactcgccg ccacgggaag acgaagtcac cgtagccgcg
     3001 catgtgcagg ttcacgttgc gccccggaat catgtctcct gcgatgccga cgtggaagac
     3061 cggctcggct tcggtcgcga gcgcgaggaa cgggtcggtg ccgtcgatgc gcagtgcggt
     3121 gacccggctg tcaagcaccg ggagtggagc gacggcgatg gtgcccgggt tgctcgccag
     3181 ctcagtcacc agcgcctcgt aatcggccac gctcgcttcc gttggcatgc cattagtggc
     3241 aatgccatcg acaccgacgc ggcggatcgg gtggggcagg ggcgagccga cttgcagcca
     3301 gttggtggcc gatccgtcga gaatcgcgct ggcactcgcg gcggtcacgt cccacagcgg
     3361 caggcgtggt gacacgacga gtgcggtccc ctgcggcagg ccagtttcct gggcagcgga
     3421 ggagccgccg aggccgagag ccgctccagc ggccatcgcg ctggcgccga tgatcagatc
     3481 gcggcgggag gcgcgtgcct cgaggacgat attgcggtcg gttcgcggct ggtcggtcga
     3541 aatcggggtc atggcttgcc gttttccaat ctcgtgtatg agcgcgag
//
LOCUS       DASYWL010000093         2223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_379448, whole genome shotgun sequence.
ACCESSION   DASYWL010000093 DASYWL010000000
VERSION     DASYWL010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379448"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VGR22_03500"
     CDS             complement(<1..563)
                     /locus_tag="VGR22_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEV2127667.1"
                     /translation="MYIIVVGGGKVGYYLTKTLLVEGHEVLLIERNRQKVDVFNERLG
                     SVVVAGDGAEASVLAEAGAQRADVVIAVTGEDEDNLVVCQMSKAKFHVPRAIARVNNP
                     KNEALFQRLGIDVTVSQTEHILNLIEQRIPEQSFVHLLNLQHDDLAIVQAKITEDSHV
                     ANKRVSEVRFPVSGVIAAIIRGPEPVMA"
     gene            complement(564..986)
                     /locus_tag="VGR22_03505"
     CDS             complement(564..986)
                     /locus_tag="VGR22_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEV2127668.1"
                     /translation="MNVVVMGSGRVGARVASILDQHGHRVTVIDINPGSLRRLGEKFS
                     GDTIIGTGIDEDVLRRAGIEDANVFIAVTNGDNRNIMAAQVARTVFNIEYVIVRIYDP
                     VREDTYRRLGLRTVCPTTTIAAIIIDQINDVLPADEEI"
     gene            complement(983..1453)
                     /locus_tag="VGR22_03510"
     CDS             complement(983..1453)
                     /locus_tag="VGR22_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV2127669.1"
                     /translation="MQTTYKVVAPVSGGSVDQRLIDALKRICARKPVEITLVYVVEVE
                     QSMPLDAELPDEVNRGESVLRDAADLVRSSVDPQIGHVYTELLQARSTGSAIVDEATD
                     KGADAIMLGATLRRQYGRLTIGDTVDYVLKHAPCEVIVIRQALPDWIAESMEWP"
     gene            complement(1577..>2223)
                     /locus_tag="VGR22_03515"
     CDS             complement(1577..>2223)
                     /locus_tag="VGR22_03515"
                     /inference="COORDINATES: protein motif:HMM:NF045129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127670.1"
                     /translation="PYRMLTSRAEFRLILRPDTADRRLSDTAASHSLISTDRHADVAA
                     ESAQIDAVIAALESSWLGDNPRHAQALTKAGLKPARRSMTAMDLARRPGVRLERVIAA
                     LEALGMWNTPSLAPHVLSRTQVAVQYSAFIDKEQREAERHKAAEDQAVPDSIDFSSVA
                     GIRVEAAQRLAERPPATIAQARRTPGVTPTDIGALLVHIRRYHGATHTSQAATP"
BASE COUNT          393 a          690 c          725 g          415 t
ORIGIN      
        1 gccatgacag gctccggacc gcgaatgatc gcggcgatca caccgctgac gggaaaccgc
       61 acctccgaca cgcgcttgtt ggccacatgc gagtcctcgg tgatcttggc ctgaacgatg
      121 gccagatcgt catgctgcag attgagcagg tggacgaacg attgctccgg gatgcgctgc
      181 tcgatcaggt tgaggatgtg ttcggtctga ctgacggtca cgtcgattcc gagccgctgg
      241 aagagcgctt cgttcttcgg attgttgacc cgggcgatcg ctcgcggcac atggaatttg
      301 gccttcgaca tttggcaaac aacaaggttg tcctcgtcct cgcccgtcac cgcgatgacg
      361 acgtcggccc gctgggcgcc cgcctcggcg agcaccgacg cctcggcgcc atcgcccgcg
      421 accaccaccg acccgagccg ctcgttgaag acatcgacct tctgccggtt tcgctcgatg
      481 agcagcacct catgtccttc aacaagcagc gtcttggtca ggtagtagcc aactttgccg
      541 ccaccgacga cgatgatgta catctagatc tcctcgtccg cggggaggac atcattgatc
      601 tgatcgatga tgatggccgc gattgttgtc gtggggcaga ccgtacgcag ccccagccgc
      661 cgataggtgt cctcgcggac cggatcgtag atccgtacga tcacgtattc gatgttgaag
      721 acggtacgcg cgacctgggc ggccatgatg ttgcggttgt cgccgttggt tacggcgatg
      781 aagacgttgg catcctcgat cccggcacgc cgcagcacat cctcgtcaat acccgtgccg
      841 atgatcgtgt cgccggagaa cttctcgccg aggcggcgga gggaaccggg gttgatgtca
      901 atcacggtca ctcgatggcc gtgctgatcg aggatcgagg cgacacgcgc cccaacccgt
      961 ccgctaccca tcacgacgac gttcatggcc actccatcga ttccgcgatc cagtcaggga
     1021 gggcctggcg gatcacgatg acctcgcacg gagcgtgctt gagcacgtag tcgacggtat
     1081 cgccgatcgt gagacgaccg tactgccggc gaagggtagc gccgagcatg atcgcatcgg
     1141 cacccttgtc ggtagcttcg tcgacgatcg ccgagccggt ggagcgggcc tgcaagagct
     1201 cggtgtagac gtggccgatc tgtgggtcaa ccgaggatcg cacaaggtcg gctgcatcgc
     1261 gtaggaccga ctcgccccga ttgacttcat caggaagttc ggcgtcgagt ggcatggact
     1321 gctccacctc gacgacgtag acgagcgtaa tctcgaccgg tttgcgagcg cagatgcgct
     1381 tgagcgcgtc gatcaggcgc tggtccactg acccgccgga aacgggcgcg acaaccttgt
     1441 aggtagtctg cacgcgagct tctgtccgcg ggcggaatga tgaacggaaa ctcaccacag
     1501 ctggaaatcc cctatctgag cgcaacaggc gatagtatcc accctgcccc tgcgaattgc
     1561 aacgaacacc agcgcgttac ggtgtcgctg cctgggaggt gtgagtcgcc ccatggtagc
     1621 gtcggatatg taccagcaga gccccgatgt ccgtcggcgt cacgccgggg gttcgccggg
     1681 cctgggcgat ggttgcgggt gggcgttcgg caagccgctg tgccgcctcg acgcggatgc
     1741 cagcaaccga actgaagtcg atcgagtccg gcaccgcctg gtcctctgcg gccttgtgcc
     1801 gctcggcctc gcgctgctcc ttgtcgatga acgcgctgta ctgcacggcg acttgcgtgc
     1861 gcgagagcac gtggggcgcg aggctgggag tgttccacat tcccagcgcc tcaagggcag
     1921 cgatgacgcg ctccagccgc acgccaggtc gtcgagcgag atccatggcg gtcatggaac
     1981 ggcgtgccgg cttgagcccc gccttggtca gtgcctgcgc atggcgcggg ttgtctccga
     2041 gccagctcga ttccagcgca gcgatcactg cgtcgatctg cgcagactcg gcagcaacgt
     2101 cggcatgccg atccgtcgag atgaggctgt ggctggcggc ggtgtcgctg agacgccggt
     2161 cggcggtgtc tggacggagg atgagccgaa actcggcccg cgaggtcagc atccggtagg
     2221 gct
//
LOCUS       DASYWL010000094         3668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_548433, whole genome shotgun sequence.
ACCESSION   DASYWL010000094 DASYWL010000000
VERSION     DASYWL010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548433"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(387..899)
                     /locus_tag="VGR22_03520"
     CDS             complement(387..899)
                     /locus_tag="VGR22_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV2127671.1"
                     /translation="MGKLIGTFTLVLMALGLLGSSSAAGHSQPGDATPTPATGITTEV
                     LGQGQPSDVPGMMLWLLRITFEPGAVAASHSHPGSSVFTIQSGSITFMLEEGTVTLHR
                     AGTIATPAAAEEISAGTEFTLNAGDTVFYESDAVFSEVNEGDEPAVILVANLRGEEEP
                     ARVPAATPAA"
     gene            complement(1105..1449)
                     /locus_tag="VGR22_03525"
     CDS             complement(1105..1449)
                     /locus_tag="VGR22_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127672.1"
                     /translation="MTDPCILICNQLNRHREVLHGALRAQRPHLRVHAVAPGEIAAGF
                     GCSDPRLIICSDPAIIQCTRPDAFILLYGDDENRAHVGIGGALRTLFDATLPQLLSVI
                     DEVWPAPDHPNE"
     gene            complement(1540..2202)
                     /locus_tag="VGR22_03530"
     CDS             complement(1540..2202)
                     /locus_tag="VGR22_03530"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2127673.1"
                     /translation="MIRLLLVDDHTAFRGALAFMLGREEDIEIAGEAGNLDEARSILQ
                     STPVDVALVDLDLSGEQGTDLVSSMRSRHPQAVAVVLTGNPRPESRASAVATGAVGVL
                     HKSTSIPSIVAAVRTAAAGQPLISPAEAVELLREAVANQARTDEGRRALESLTDRERD
                     VLRALATGLDNQAIADRLHISHETVRSHIVRILRKLGVESRLQAVLFALRYGFLDPHD
                     IG"
     gene            complement(2199..>3668)
                     /locus_tag="VGR22_03535"
     CDS             complement(2199..>3668)
                     /locus_tag="VGR22_03535"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV2127674.1"
                     /translation="VFLAIGQIFGAFVLAISPLEPARIRLRWVAAGFLVFGVGALAYG
                     YLYPLLVDSPHLTATMYGSLYVRTVGTALCAAGLALGNLPRIERTILILLTAAAILCG
                     ALLVPLADHLPPLIRISGTTEIIDHDPVIDATDPADHVHLSDLEALLSDTPRTVPGLT
                     AWHWTLAIIPLVASLIAVLGTTLRAALRPIGAWLLLALALLAAAQLHSVFWPSMYSSI
                     LTTTSILRAGTALMVIIGGVLELRHIILQRDALLAAEQERTQRLEDLAALKADFTSIV
                     AHELASPVAAIDTMSQMMSIDDLPPDLRRKTAEDIQAEARLLQMLVLDVRESANIERD
                     DFRVVPRRLSLDTLVSEAESYARNLPGSHPVTIEHTAHTDVRGDPDRIGQVVRNLLNN
                     AAKHTPDSTPIIIRTRREDSDVVIEVADRGPGIQPEDLGRIFEKFGRGREAARRHASG
                     RGLGLYLSRRILHGHQTDLEVESRPGHGTAFRFRLREFV"
BASE COUNT          601 a         1101 c         1329 g          637 t
ORIGIN      
        1 cagtaccacc gactcgacgg cggctacggt agccacgccg taccttgata ttatggcgac
       61 cacgaccggt ggtaaggcga ggcggtgtcg catccgcctg agggcctggc ttccgccggc
      121 ggcacaactc ctttagcacg agcgccccgc accagacctc cgcggggcgc tcgtgtgtca
      181 gtgccccttc cagaagcgca ccagacgtgc ccagacgccc ccctgctgcc cgttctccgc
      241 gccgacgtct cagcccccca ccctgtcggc cttcccgctc atcatggagc aagccgtacg
      301 cggtactgac cagaggacgc cgtggaacgc cccttggccg gatgaccgag gggcgtcgtg
      361 ccaggtgtgc caggaggaac agccgattac gcagccggtg tcgcagccgg cactcgggct
      421 ggctcctcct cgccgcgcag attggcgacc aggatgacgg ccggctcgtc accctcattg
      481 acctcagaga agacggcgtc ggattcgtag aagacggtgt cgcccgcgtt gagcgtgaac
      541 tccgtgccgg cgctgatctc ctcggcggcg gctggcgtgg cgattgtccc ggcgcgatgc
      601 agggtcaccg tgccctcttc cagcatgaat gtgatcgagc cggactggat cgtgaagacg
      661 ctggaccccg gatgcgagtg cgaggccgcg acggcacctg gttcgaaggt gatgcggagc
      721 agccacagca tcatgcccgg gacgtctgat ggctggcctt ggccaaggac ctcggtggtg
      781 atgccagtgg ccggagtggg ggtggcatca ccaggttggc tgtggccggc cgcgctggag
      841 gagccgagca gcccgagggc catgaggacg agcgtgaagg taccgatgag cttgcccatt
      901 gtgaaaactc ccttgaggtg atgatcggtg gtggcccagg tgggcgctcc atgtgttgcc
      961 cccgggcgcc cgcccgggct ttgtcggaca cctccctcgg gctttcgccg ctccccacct
     1021 gcaggccggg gtggtcggtt gtcctttgct ctagattagg gatttggcaa gatgggcgaa
     1081 acgcgcagat gagtgaaatg ggggtcactc atttgggtga tccggtgccg gccagacctc
     1141 gtcgatgacg gagagcagct gcggcagcgt ggcgtcgaag agcgtccgca gcgcgccgcc
     1201 gatgcccacg tgtgcgcgat tctcgtcgtc gccgtagagc aggatgaacg cgtcggggcg
     1261 cgtgcactgg atgatcgccg gatcgctgca gatgatgagg cgcgggtcgg agcagccgaa
     1321 tccggcagcg atctctccag gggcgaccgc gtgcacgcgc aggtgcgggc gctgagcgcg
     1381 cagggcgcca tgcaggacct cccggtgccg gttcagttgg ttgcagatga ggatgcaggg
     1441 gtcggtcacg tggtgcctcc gtccgttgcg gtcaaggcac catcgtatgg gctggtgagg
     1501 tcggagtcgc cctccgaatg agggaatcgc gggggcgtat caaccgatgt cgtggggatc
     1561 gaggaagccg tatcgcaggg cgaagagcac ggcttggagc cgggattcca cgcctagctt
     1621 gcgcaggata cgcacgatgt ggctacggac cgtctcgtgg ctgatgtgga gacgatctgc
     1681 aatggcctgg ttgtcgagcc cggtcgcgag ggcgcggagc acgtcgcgtt cgcggtctgt
     1741 gagactctcc agcgcgcgcc ggccctcgtc ggtccgggcc tggttggcga cggcttctcg
     1801 gagcagctca accgcttcgg ctgggctgat gaggggctgt cctgccgctg ccgttcgaac
     1861 tgcggcgacg atgctcggga tggatgtcga cttgtgcagc accccgacgg ctccggtcgc
     1921 tacggccgac gcacggctct cgggtctcgg gttcccggtc agcaccactg ctacagcctg
     1981 ggggtggcgc gaccgcatgc tgctgaccag atccgtgccc tgctcgccgc tgaggtcgag
     2041 atcgaccagc gcgacatcaa caggagtcga ttggaggatc gatcgcgcct cgtcgaggtt
     2101 gcctgcctcg cccgcgatct cgatgtcttc ctcgcggccg agcatgaacg ccagcgcacc
     2161 gcgaaaggcg gtgtggtcat cgacgagcag caggcggatc atacgaattc cctcaatctg
     2221 aatcggaacg ccgtgccgtg gccgggccgg gattcgacct ccagatcggt ctggtgcccg
     2281 tgaaggatgc gtcgcgagag gtaaagcccc agccctctgc cgctggcgtg ccggcgtgcg
     2341 gcctctcgcc cccggccgaa cttctcgaag atgcggccga ggtcttctgg ctgaatgcca
     2401 ggcccgcggt cggccacctc gatcacgaca tcgctgtcct cacggcgggt ccggatgatg
     2461 atcggggtgc tgtcaggcgt gtgtttcgcc gcgttgttca gcagattgcg caccacttgg
     2521 ccgatacggt ccgggtcgcc acggacatcc gtgtgcgctg tgtgttcgat cgtgacgggg
     2581 tgggatcccg ggagattccg tgcgtaggat tcggcctccg agacgagtgt gtcgagtgat
     2641 aatcggcgag ggacgacccg gaaatcatct cgctcgatgt tggctgactc gcggacatcg
     2701 agcaccagca tctggagcag gcgggcttcc gcttggatgt cttcggcggt cttgcggcgc
     2761 agatcggggg gcaggtcgtc gatcgacatc atctgcgaca tcgtgtcgat cgccgcgact
     2821 gggctcgcca gctcgtgtgc gacgatcgag gtgaagtccg ccttgagtgc agccaggtct
     2881 tcgagtcgct gcgttcgctc ctgttcggca gcgaggaggg cgtcgcgctg gaggatgatg
     2941 tggcgcagct cgagaacgcc tccaatgatg accatgaggg cggtgccggc tcgcaggatg
     3001 ctggtcgtgg tgaggatgga gctgtacatg gacggccaga agaccgagtg aagctgcgcg
     3061 gcggcgagga gtgccagcgc gaggagtagc cacgccccga tcggccgcaa cgcggctcgc
     3121 agcgtcgtac ccaggacagc gatcaggctt gcaacgagcg ggatgatcgc gagcgtccag
     3181 tgccaggccg tgagtcccgg aaccgttctc ggcgtatcgg acaggagcgc ctcgaggtcg
     3241 ctgagatgga cgtggtcggc cgggtcggtg gcatcgatga cgggatcgtg atcgatgatc
     3301 tctgtggtgc cggagatgcg gatcagcggt ggcaggtgat ccgcgagtgg taccaggagg
     3361 gcgccgcaga gaattgccgc tgcagtgagc aggatcagga tcgtccgttc gatgcgaggg
     3421 aggttgccca aggcgagtcc tgccgcgcac aaagcggtgc ccaccgtccg cacgtagagc
     3481 gagccgtaca tggtggcggt cagatggggc gagtcgacga gcagcggata gagataaccg
     3541 taggccagtg cgccgacgcc gaagacgagg aagccggctg ctacccagcg gagccgtatg
     3601 cgggcgggct cgaggggtga gatcgcgaga acaaaggcgc cgaagatctg gccgatggcc
     3661 aggaagac
//
LOCUS       DASYWL010000095         4818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_510685, whole genome shotgun sequence.
ACCESSION   DASYWL010000095 DASYWL010000000
VERSION     DASYWL010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510685"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..832)
                     /locus_tag="VGR22_03540"
     CDS             complement(29..832)
                     /locus_tag="VGR22_03540"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2127675.1"
                     /translation="MPETILDRTPPPADRRISWGDGRLQFADLRRPEGDGPHLAAIAI
                     HGGFWRNRYSLDYMGHLCAALTAQGVVTWNIEYRRVGDPGGGWAGTFQDVVAASRYLF
                     SHAEEFSVDRERIIVLGHSAGGHLVSWLASLGNVPSDSEIAADPLPLAAAIPIAGVLD
                     LARCWELHLSDDAVVDFLGGTPGEVLERYAAASPIVLVPPSVPSLIIHGQYDENVPIE
                     LSERYHAALLAAGALSAMPLLPRVDHFDVVDPESRAWPTIARAIGSLFD"
     gene            complement(1008..2243)
                     /locus_tag="VGR22_03545"
     CDS             complement(1008..2243)
                     /locus_tag="VGR22_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127676.1"
                     /translation="MIDESTRPPLDAISDAYVRLAFRMEPHIEGLIDAYNGPVTMKEH
                     AEASPATPAELVREADVLLERIATDEELAPSRVDYLTAQVTALATLARKLTGKEIAYV
                     DEVRQLFDVEAVRVPDDAYAAAITTLDEALPSEGPVPARLQRWKTQFELTTEQARLAV
                     ETILDEVRTRTAKMVSLPEGEAVEIAFVSDKPWNGYNWYLGDARSLVEINTDLPIRAN
                     ALLDLMTHEGYPGHHTEHALKEVRLYHEKGYGEHSIQLINTPECVISEGIATLAQSMI
                     FGVEEGTRWQAEHVWGPFGIDADPAREARIIDAQWVLRSVGGNAALLMYQEGRPEEDV
                     VRYLMEYGLATEEEARHRVQFIADPLWRPYIFTYHVGRDLLARWLDEAEVNGETRESR
                     FMRLLEEQFTPGAIARDLG"
     gene            2369..3559
                     /locus_tag="VGR22_03550"
     CDS             2369..3559
                     /locus_tag="VGR22_03550"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HEV2127677.1"
                     /translation="MKALTWHGKRDVRVDTVPDPTIKEPTDAIIRITSTGLCGSDLHL
                     YELLGAFLEEGDILGHEPMGIVEEVGSTVTEIKPGDRVVIPFNVACGHCFMCERGLQS
                     QCETTQVHKHDKGAALFGYTKLYGQVAGGQAEYLRVPQAQYTHIKIPEEGAALPDDRF
                     VYLSDVLPTAWQAVEYAQIPEGGTVAVLGLGPIGDMSARIAKHRGHRVIAVEIVPDRI
                     RRGRENGIEVIDGKNTKKVGDAIREMTNGRGPDSVIDAVGMEAHGAPVSRLAQEMATF
                     LPDPIASPLMQRAGVDRLAALLLAIDVVRRGGTISIIGVYAGMTDPLPMMTMFDKQVQ
                     LRMGQANVKRWSDDIMPLLTRSDDPLGVDTFATHRLPLDDAPRAYEMFQKKQDGAVKV
                     LFQP"
     gene            3596..4084
                     /locus_tag="VGR22_03555"
     CDS             3596..4084
                     /locus_tag="VGR22_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127678.1"
                     /translation="MSKYLRSVPIRALMIGIAAGMRSQIPGAVLAWRQDDAPFYASWR
                     RWPVLRSVWGRRVLMLSAAGEVIADKTSAVPPRTKPGPLFGRMLFGGIAGAAIGSERR
                     GKGPLVRGALLGVLGGLIGSYGGMRARASVAQITGLPDQVVAVFEDVAAVSIANRAIT
                     KR"
     gene            complement(4330..4767)
                     /locus_tag="VGR22_03560"
     CDS             complement(4330..4767)
                     /locus_tag="VGR22_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV2127679.1"
                     /translation="MTDRPPIEIADTWFRHLAERDVDAVMSLAADDIEVGGARGSGSD
                     MELLREWVTRMKAVVIPRRWFTKDDVVVVEQEAVWYSRAGEDMGRRVMITTFRIENGH
                     IGGIYRHDDLAAAVTIGGLDASHELDPPEGTGTQARPVLGDDS"
BASE COUNT          816 a         1597 c         1597 g          808 t
ORIGIN      
        1 cgttctggct gcggcaatcg atgcaggttc agtcaaacag gctgccgatc gcccgcgcaa
       61 tcgtcggcca agcccgcgat tcggggtcga cgacgtcgaa atgatcgacc cgcggcagca
      121 gcggcatcgc cgacagcgcc ccggccgcca gcaatgccgc gtggtacctc tcgctgagct
      181 cgatcggcac gttctcgtcg tactgaccgt ggatgatgag cgatggaact gacggcggca
      241 ccagcacgat cggcgaggcg gcggcatagc gctccagaac ctcccccggc gtgccgccga
      301 gaaaatccac cacggcatcg tcgctcaggt ggagctccca gcagcgtgcg aggtcgagca
      361 cgccggcgat cgggatcgcc gccgcgagcg ggaggggatc agcggcgatc tcgctgtccg
      421 acggcacgtt gccgaggctc gccagccagc tcacgaggtg tccgccggcg gagtgcccga
      481 gcacgatgat gcgctcccga tccacgctga actcctcggc atggctgaag aggtagcgcg
      541 aggcggcgac gacatcctgg aaggtccccg cccagccacc gcctgggtcg ccgactcggc
      601 gatactcgat gttccaggtg acgactccct gcgcggtcag tgctgcacag aggtgcccca
      661 tgtagtcgag ggagtagcga ttgcgccaga agccgccgtg gatggcgatc gccgccaggt
      721 gcggcccgtc gccttcaggg cggcgcaagt cggcgaactg caaccgaccg tcgccccacg
      781 agatgcgcct gtcggcgggc ggtggcgtac ggtcgaggat cgtttccggc atgagcgtct
      841 cgttgcgcgg tgcgaagcga cggtcgagca agttggctcg atccgatcat aaaggacgcc
      901 aggagttgag ccctcgccgc cgtacagaag gagattgctc catgcggccg gacgacgctt
      961 ggccgaaatg ccaagccagc caccgccacc caccgagagc gctctgacta cccgaggtcg
     1021 cgggcgatgg cgcccggcgt gaactgctct tccagcagcc gcatgaagcg tgattcccgc
     1081 gtctcgccgt tcacctccgc ctcgtcgagc cagcgtgcca gcagatctcg accgacatgg
     1141 taggtgaaaa tgtagggccg ccagagcggg tcggcgatga actgcacccg gtgccgtgcc
     1201 tcctcctcgg tcgcgagccc gtactccatc aggtagcgaa ccacatcctc ctccggacgc
     1261 ccctcctggt acatcagcaa cgcggcgtta ccgccgaccg accgcagcac ccactgtgcg
     1321 tcgatgatcc gcgcctcccg ggcaggatca gcgtcgatac cgaagggacc ccaaacatgc
     1381 tcggcctgcc atctggtgcc ctcctccacg ccgaagatca tcgattgtgc cagcgtcgcg
     1441 attccctcgg agatcacgca ctccggcgtg ttgatcaact ggatgctgtg ctcgccatag
     1501 cccttctcgt ggtagagacg gacctccttg agcgcatgct cggtgtggtg acccgggtac
     1561 ccctcgtggg tcatcaggtc gagcagcgcg ttggcgcgga ttggcagatc ggtattgatc
     1621 tcaaccagcg agcgcgcgtc gccgagatac cagttgtacc cgttccacgg cttgtcactg
     1681 acgaacgcga tctcgaccgc ctccccctcc ggcaggctga ccatcttggc ggtgcgggtg
     1741 cggacctcgt ccagaatcgt ctcgaccgcc agtcgcgcct gctcggtggt gagctcgaac
     1801 tgggtcttcc agcgctgcag tcgcgccggt accggtccct cgctaggcag cgcctcgtcg
     1861 agggtggtga tcgcagcggc gtaggcgtcg tccggcacac gcacggcctc gacgtcgaaa
     1921 agctggcgaa cctcatcgac gtaggcgatc tccttgcccg tcagcttccg ggcgagcgtc
     1981 gccagcgccg taacctgcgc ggtgaggtag tcgacgcggg aaggcgcaag ctcctcgtcc
     2041 gtcgcgatcc gctccagcaa cacgtccgcc tcgcgcacca gttccgccgg tgtggcgggc
     2101 gaggcttcgg catgctcctt catggttacg ggaccattgt aggcgtcgat cagaccctcg
     2161 atgtgcggct ccatccgaaa ggcgagccgc acgtaggcgt cggagatcgc gtcgagtggc
     2221 ggtcgggtgg attcgtcgat catggcacgt cctcaagggg aagggcgtga gcagacgatc
     2281 gccatggtac cgcacccgga tgtggcatgg aacctgcatt cggtccccgg acagtgcagg
     2341 caggtacggc ccatctgaag gacatggcat gaaggcactc acttggcacg gcaagcgcga
     2401 cgttcgcgtc gatacggtcc ccgatccgac catcaaggag ccgaccgacg cgatcattcg
     2461 catcaccagc actggcctct gcggctccga cctgcatctc tacgaactgc tcggcgcgtt
     2521 cctcgaggag ggagacatcc tggggcacga acccatgggc atcgtcgagg aagtcggcag
     2581 cacggtcacc gagatcaagc cgggcgaccg cgtggtgatc cccttcaacg tcgcctgcgg
     2641 ccactgcttt atgtgtgagc gaggcctgca gagccagtgc gagacgaccc aggtgcacaa
     2701 gcacgacaag ggcgccgcgc tcttcggcta caccaagctc tacgggcagg ttgccggcgg
     2761 gcaggcggag tatttgcgcg tgccgcaggc gcagtacacc cacatcaaaa taccggagga
     2821 aggcgccgcg ctgcccgatg accgcttcgt ctacctctcc gacgtgctgc cgaccgcttg
     2881 gcaggccgtc gagtacgcgc agattcccga gggcgggacc gttgccgtgc tcggcctcgg
     2941 gccgatcggg gacatgtccg cccggatcgc caagcaccgc ggccaccggg tgatcgccgt
     3001 cgagatcgtc ccggatcgca tccggcgcgg acgcgagaat ggcatcgagg tgatcgacgg
     3061 caagaacacg aagaaggtcg gtgacgcgat ccgggagatg acgaacgggc gcggccccga
     3121 ctcggtgatc gatgcggtcg gcatggaggc ccacggcgcg ccggtcagca ggctggcgca
     3181 ggagatggcc acgttcctgc cggatccgat cgcgtcgccg ttgatgcagc gggccggggt
     3241 cgatcgcttg gcggcgctgc tgctcgccat cgacgtcgtc cggcgcggtg gcacgatctc
     3301 gatcattggc gtctacgcgg gcatgacgga tccgctgccg atgatgacga tgttcgacaa
     3361 gcaggtgcag ctgcggatgg gacaggcgaa cgtcaagcgc tggtcggacg acatcatgcc
     3421 gctgctgaca cgctccgatg acccgctcgg cgtcgacacc tttgcgacgc accggttgcc
     3481 gctcgacgac gccccacgcg cctacgagat gttccagaag aagcaggacg gtgccgtcaa
     3541 ggtcctcttc cagccgtagc agattcccgc ccggttcgcc cgtcaggaga cgtcgatgtc
     3601 caagtatctt cgttccgttc ccattcgtgc attgatgatt ggcattgccg ccggcatgcg
     3661 ctcgcagatt cccggtgcag tactggcctg gcggcaggac gatgccccgt tctatgccag
     3721 ctggcgacgc tggccggtac tgcgcagcgt ctgggggcgt cgcgtgctga tgctctccgc
     3781 tgccggtgaa gtgatcgcgg ataaaacgtc ggcggtgcca ccgcgcacga agcccggtcc
     3841 ccttttcggt cggatgctct tcggcgggat cgcgggggcg gcgatcggct ccgagcggcg
     3901 cggcaaggga ccactcgtcc gcggggcgct gcttggcgtg ctcggtgggc tgatcggcag
     3961 ctacgggggg atgcgcgccc gtgcgagcgt cgcgcagata accggtctgc ctgatcaggt
     4021 cgtcgccgtg ttcgaggatg ttgcagcggt gtcgatagcc aaccgggcga ttacgaagcg
     4081 gtaatcgccg tcactcgcag cgttcagcaa gagaggcgct gggtgtcacc gttgcgtcgg
     4141 tcccgtaaaa tctgcacaac attctcgtac agaaattccg gttgcggagt atccgtcagt
     4201 ggttttagcg gcccctccag gactgttggc gcgtctgacc tgagggcagt gcagtcgcga
     4261 gacgcccgtc gcgcagaccc cttcgactcg ggcctgcggc cctcgctcag ggccggctcc
     4321 ggtccacctc tacgagtcgt cgccgagtac aggacgcgcc tgtgtgccgg taccctccgg
     4381 aggatcgagc tcgtgcgagg catcgagacc gccgatggtg acggcggccg cgaggtcgtc
     4441 atggcggtaa atgccgccga tgtggccgtt ctcgatgcgg aacgtcgtga tcatcacgcg
     4501 ccggcccatg tcctcgccag cacggctgta ccagaccgcc tcctgctcca ccaccacaac
     4561 atcgtccttc gtgaaccagc ggcgcgggat gacgacggcc ttcatccgag tcacccactc
     4621 ccgcaacaat tccatgtcgg agccagaacc gcgcgcaccg ccgacctcga tatcgtcggc
     4681 ggcgagcgac atcacggcgt cgacgtctcg ctcggcgagg tgccggaacc aggtgtcggc
     4741 gatctcgatc gggggacggt cggtcatgcg gggcatgctc ctgcggctgg cgccagatca
     4801 ctccacagag gatacagg
//
LOCUS       DASYWL010000096        11476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_248607, whole genome shotgun sequence.
ACCESSION   DASYWL010000096 DASYWL010000000
VERSION     DASYWL010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 11476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11476
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248607"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..375)
                     /locus_tag="VGR22_03565"
     CDS             complement(226..375)
                     /locus_tag="VGR22_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127680.1"
                     /translation="MISCSLARRRGRTVPTGIALHELEHHVGVDLREVENRNVLGLVI
                     EDAMG"
     gene            374..727
                     /locus_tag="VGR22_03570"
     CDS             374..727
                     /locus_tag="VGR22_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127681.1"
                     /translation="MQFVHHAEENGLFTADEAFDHAVLMKVLPKFNGSRGKLQQALFE
                     LIHWCRTPAILNSEGRSAYESRLTKFQEDGKSFDAIAISDADTVDENWRYPATGRRAS
                     LMMQDLYTDGFASFG"
     gene            820..1104
                     /locus_tag="VGR22_03575"
     CDS             820..1104
                     /locus_tag="VGR22_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127682.1"
                     /translation="MTIGDMLQRVQRLFFGTKPASAKQAQAARVRRHEVMLALQQEVR
                     QLQQEISDLSDREGIDPDGGWSEDESPEMASLHRQLSRKQAELAKYQARI"
     gene            1188..2120
                     /locus_tag="VGR22_03580"
     CDS             1188..2120
                     /locus_tag="VGR22_03580"
                     /inference="COORDINATES: protein motif:HMM:NF013397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEV2127683.1"
                     /translation="MSRRHSHPYTAEPEPAEDLRDSEVEDDEDEQAELSVEEQPKPLG
                     QDAPEVVEDASKIGAQRLRRPLLGDAITSFTGGMSVGFGAVAFAAGAAAVGGTLGSPS
                     VGLLVGSLLFPIGFLILLVGKTELFTENFLLPVAAVIKERGNLVQLGSLWSVTLGANL
                     LGALAFAVLISWDGVLAESVADQLVILAEHKVAYVLPTAFIKAIFAGWLMTTLTWLLV
                     ASAGGMGPRIAVIWGIAALIVLGQFNHVIISAAEIFAAMMLDANITIGMWFTDNFLPA
                     LTGNVLGGLIFETMLQTVQARYQEPDEEEQPQNA"
     gene            2402..2860
                     /locus_tag="VGR22_03585"
     CDS             2402..2860
                     /locus_tag="VGR22_03585"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEV2127684.1"
                     /translation="MTEDSPMGIAGCLRRPGRRLAVAVMFWTGVAIALVWWPEPLVWL
                     GVALGALLPVSVWIVWNRPHLYREAPRLEDFLAMTLRQFEEAVADLIVPLGYTDVRVV
                     GGAAGLGVDVPCRDRKRRKVAIQVKRYSPGEPDRLIGGADVHGRDGRARG"
     gene            3302..3874
                     /locus_tag="VGR22_03590"
     CDS             3302..3874
                     /locus_tag="VGR22_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127685.1"
                     /translation="MGRSILASIASVAGAFALVAAMLLSGGLATAQDGTPPPEFTEDD
                     VPRPAHIHSGTCEELGDVVYPLGDLTGVTIQGSPVASPIVENPESPDIVQGEVVIESI
                     TEVETTFDELFGAEHAINVHDSAENIGLYIACGNIEGEPEDGRLEIDIHPLNNSGFEG
                     IAVLEETEEGVITVTVTLRDVSDVEATPAL"
     gene            complement(3954..4301)
                     /locus_tag="VGR22_03595"
     CDS             complement(3954..4301)
                     /locus_tag="VGR22_03595"
                     /inference="COORDINATES: protein motif:HMM:NF019401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEV2127686.1"
                     /translation="MEFQKTVEVVGKAVEATGVAVIVIGILLAGIRNAGAAFGDEPHR
                     GFRRVRQAVARSILLGLELLIAGDIIRTVAVDPTFTSVGVLALIVLVRTFISIELELE
                     ISGRWPWQRKEEQ"
     gene            complement(4386..5138)
                     /locus_tag="VGR22_03600"
     CDS             complement(4386..5138)
                     /locus_tag="VGR22_03600"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV2127687.1"
                     /translation="MVALQALAGLVLFNALLLLGLGRTTAVASGIITSSTPAVIALMS
                     VALGDCLKPGGWLGVALAVLGIVIVNLFGTAEASSATQPFLGAVLVFLAVVGKALYTV
                     LGKIAAPSLRPVPMATLVTVYGFLLFLPLAATDVGDMRPSEIPGSGWLALLYLAAIVT
                     VVAFALWFQGLQHVPASTAGALTGMIPVGTILATAIFLDEPVTWLHILGVTVVVVGIA
                     LVTQGIRPARHAVPPGSTAGMPTYPNEPSPAP"
     gene            5180..5386
                     /locus_tag="VGR22_03605"
     CDS             5180..5386
                     /locus_tag="VGR22_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127688.1"
                     /translation="MAHQGEQDDPGEEEPQGGRGVDRHRVDHDLAGDDRRPHDEHRAR
                     EEEVAGDGDVVESANAAIAPDPVG"
     gene            5498..5929
                     /locus_tag="VGR22_03610"
     CDS             5498..5929
                     /locus_tag="VGR22_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127689.1"
                     /translation="METTTQIAIARRIDMIAEWRAEREHQDMLGLGPEAARRSRRSAE
                     GLRELANHIAGLPDDHPHIVRLRELAFAGGQFDPGPMLLNELGRFRFHEPEQRVEVFL
                     EKMVEYAEQDASEIALFGGPQVPGDNPWIIRIIDDEDDWDA"
     gene            complement(5978..7360)
                     /locus_tag="VGR22_03615"
     CDS             complement(5978..7360)
                     /locus_tag="VGR22_03615"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEV2127690.1"
                     /translation="MTDIPLPDTPPQVTLGGIFHEEHQCAYEHVPFEVPESVDQLLID
                     VGYNDRIDSSPLARSGNTLDIGLFDERGIESRSPGFRGWSGSVQTRIVVGTERATPPY
                     RAGKPGAGTWHMLLGPYKIGQNGLRWEVRIWLNPEIALPDEKHAPDIDSVWRPYLPAP
                     AEPNWYRGDLHVHTVYSDGTATPAEIAVTAVEAGLHFYGITDHNRAQSPVGLVPQGDE
                     WPVLVPGVEVTTYAGHFNVWGTEHWYEFRDPSAEGLQGAVDAALRDGGTVSLNHPKPF
                     GPEWEFPEVTGFHVIEAWNSWWARFNAASLEWWHRQLGSERPLPMVAGSDMHEHRLFG
                     APDRPLEPTRIGWPTTWAEVDGELTAEAVLAAVREGRTFVSESPSGPQLYNLGDGSEV
                     RMRIAGADGQALLLIGPQGVVHAEGITGDDVESFVPSSRLPGDLPFVRPEIHAPGGAV
                     KALGQAIWLR"
     gene            7608..9020
                     /locus_tag="VGR22_03620"
     CDS             7608..9020
                     /locus_tag="VGR22_03620"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV2127691.1"
                     /translation="MSSDQQEQRVENEGPVAQFVSVDETLHAPSFNGGKPRVVIVGGG
                     FAGLNAAKALDGAAVEVLLIDRRNHHLFAPLLYQVATAQLSPANIAQPIRGILRRQKN
                     LSVLLAEVVGVEVNGRRVRLDDGRTVDYDYLILAAGATHSYFGNDQWAPLAPGLKTIE
                     DALTIRRRILLAFEDAEREPDPARRASMLTFVIVGGGPTGVELAGAIGELANHTLARD
                     FQHIDPRDAHILLLEGLPQILPMFNEKLARQAVEDLERFGVEVRTNSLVTAIDPEGVN
                     VGDERIPTHNVFWAAGVSAAPVTRSLAGVAELDRAGRATVTRELNIPGHPEIFVIGDA
                     ASAPGENGKPLPGVAPVAIQEGQWVACNMRAALEGNAYAPFRYRDQGSMATIGRNRAI
                     ADVKGLEFTGFVAWLAWALVHVAQLIGFRNRLMVGAQWLFNYFTYRRGARLITGELSD
                     ERRKDMMARLGKRSGELPAR"
     gene            complement(9136..10410)
                     /locus_tag="VGR22_03625"
     CDS             complement(9136..10410)
                     /locus_tag="VGR22_03625"
                     /inference="COORDINATES: protein motif:HMM:NF039815.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127692.1"
                     /translation="MNQSGVHGRWATAIISGALIAVIGVATVLAQALGPAGPSPAEDN
                     AAVVAHAVVELPAGETVWRIRNLQIDGDTAPISGDVPALLTTDGVPVLVEDLSTGLRQ
                     RVASGEGAVLVPGNESSVTSMGPPQSVLAVDVMPVDEASLSGSPGRISVPFQVEGGSY
                     DVDLIRIRLDEGESSTVPMGNGQSLIVVRSGQVDVQAEDEQFSMTAGGDRLASGELTI
                     TATSDEAAVVVARIGPAIESIEEPATPVASQELETPEATPEPTATPVPATPVATPSPA
                     TPVAEEPQDIDSDGDGVDNADEIAAGTNPDDPDTDDDGLFDGVEIELGTDPLSADTDE
                     DGILDGDEVFSAGTDPLNSDTDGDVLYDGGELLYNTDPLVVDTDVDGLTDGDEVYFSE
                     TSPTAVDTDGDGRTDYDEVVAGTDPLDANSFP"
     gene            complement(10570..11184)
                     /locus_tag="VGR22_03630"
     CDS             complement(10570..11184)
                     /locus_tag="VGR22_03630"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEV2127693.1"
                     /translation="MAYELPALPYAFDALEPHIDAQTMEIHHDRHHGTYVQKVNDAIA
                     GTDLEGLSIEELMKNLDRVPEAKRAAVRNNGGGHYNHSLFWTVMGPNGGGQPSGALAD
                     TINEAFGSFDALKEEMNTAGANRFGSGWAWLVVDGTGKLAVTSTPNQDNPLMDGSGTP
                     ILGVDVWEHAYYLKYQNKRPDYLNAWWNTVNWDAVTNNYRKVRG"
BASE COUNT         1911 a         3635 c         3876 g         2054 t
ORIGIN      
        1 gaggtggtca gcccgaagct gggcgacgcg tcgacgtcat tggacttctt ctcggcaatg
       61 ctggccgacc tcacctcgga ggagggcgcg ctcgcgttca tgccgctggc acagacctcg
      121 cggactgtcc agcaagtcca acggccgccg tcgctgctca tccagtactt cttcctcgtc
      181 aacaacgcgg acggcatcgt cgacgcgctg gagctcctgc ggcgctcacc ccatcgcatc
      241 ctcgatgacg agaccgagca cgtttcgatt ttcgacctca cggaggtcga cgccgacgtg
      301 gtgttccagc tcgtgcaggg cgataccagt tgggaccgtt cgacctctcc tccgcgcaag
      361 gctgcacgag atcatgcagt tcgtccatca cgccgaggag aacgggctct tcacggccga
      421 cgaggccttc gatcacgccg tgctgatgaa ggtgctgccg aagttcaacg gctcgcgcgg
      481 caagctgcag caggcgctct tcgagctgat ccactggtgc aggacgccgg cgattttgaa
      541 ctcagagggg cgatccgcgt acgagtcccg gctcacgaag ttccaggaag atggcaagag
      601 cttcgatgcc attgcgatct cggatgcgga caccgtcgat gagaactggc gctacccggc
      661 gaccggtcgt cgggcctccc tcatgatgca ggacctctac accgacggct ttgcctcctt
      721 tgggtaggcc cgagccgatc acgcgccatc agcagtgcgt gaacccacta cactcaggag
      781 caacaccggc ccgttctcga aaaacgatcg aggtgcaccg tgacgatagg cgatatgctt
      841 cagcgggtcc agcggctttt cttcggcaca aaaccggcaa gcgcgaagca ggcacaggcc
      901 gcccgcgtgc gccgccatga ggtgatgctc gcgctccagc aggaggttcg ccagctgcag
      961 caggagatct ccgacctcag cgatcgcgag gggatcgacc ccgatggtgg atggtcagag
     1021 gacgagtcgc cggagatggc atccctgcac cggcagctga gcaggaagca ggctgagctg
     1081 gccaagtacc aggcgcgaat ctgacgagga acctgacccg attcgggtcc cgttccggct
     1141 ggtcaccatc ttgcgtatcc catccggata tgaaagggac gcgtactgtg agccggagac
     1201 attcacaccc atataccgct gagccagagc ccgccgagga tctccgtgac tcggaagtgg
     1261 aagacgacga ggacgagcaa gcggaactat cggtcgaaga gcagccgaag ccgctcggcc
     1321 aggatgcacc cgaggtcgtc gaggacgcgt cgaagatcgg cgcccagagg ctgcggcggc
     1381 cattgctggg tgacgcgatc acctccttca cgggcggcat gagcgtcggg ttcggcgccg
     1441 tggcgttcgc ggcgggggca gccgcggttg gcggtaccct cggctctccc tccgtcgggc
     1501 tgctggtggg ctcgctcctg tttccgatcg ggttcctgat cctgctggtc ggcaagacgg
     1561 agctgttcac cgagaacttc ctgctgcccg tcgcggcggt gatcaaggag cgggggaacc
     1621 ttgtccaact cggcagttta tggtcggtga ccctcggcgc caacctcctg ggggcgctgg
     1681 cgttcgctgt gctgatctcg tgggacgggg tgctggccga atcagttgcc gaccagctcg
     1741 tgatcctcgc cgagcacaag gtggcgtatg ttctccccac agccttcatc aaggcgatct
     1801 tcgcgggttg gttgatgacg accctgacct ggctgctggt cgcgtccgcg ggtggcatgg
     1861 gtccgcgtat cgctgtcatc tggggcatcg ccgcgttgat cgtgctgggg cagttcaacc
     1921 atgtcatcat ctccgctgca gagatcttcg cggcgatgat gctggacgcg aatatcacga
     1981 tcggtatgtg gttcaccgac aacttcctgc cggcactgac gggcaacgtg ctcggtggac
     2041 tcatcttcga aaccatgctg cagaccgtgc aggcacgcta tcaggagccg gatgaggaag
     2101 agcaaccgca gaacgcctga tggcccgtcg ggtcgtacac ggcttcggag agcatgctgc
     2161 cgcgtctgca tatgctgcga cactccccgc cacgacatct taagagtcgc tcagggaagg
     2221 ccggtttgct tggtgcgcgc tgaggcgtct cgatgtgatc gtaccctggg ctccaagtcc
     2281 ccctgcaggt caggtgcggg aaccccctcg tgctggcggc tcctcctgta cgtcggtcca
     2341 attgcccgat ccctgtttct cctcgcacaa tggcgtttga tcgggtcatc ggaacaggaa
     2401 cgtgacggag gattcgccga tgggcatcgc cggctgtctg cgtcggcctg ggcgccggtt
     2461 ggcggtcgcc gtgatgttct ggacgggcgt cgccatcgca ctcgtctggt ggcccgagcc
     2521 gctcgtctgg ctcggtgtgg ctctcggcgc gctgctgccg gtgagcgttt ggatcgtttg
     2581 gaaccggccc cacctttacc gggaggcgcc gcgtctcgag gactttcttg ccatgacact
     2641 gcgccagttc gaggaagctg tcgcggacct gatcgtgccg ctcggctaca ccgacgtgcg
     2701 tgtggtcggc ggtgcggcgg gtctcggcgt cgacgtgccg tgccgcgacc ggaagaggcg
     2761 caaggtggcg atccaggtca agcgctacag ccccggagaa cccgatcggc tcatcggcgg
     2821 tgcagacgtt catgggcggg atggtcgcgc acgaggctga gtggggcatc atcgtgacga
     2881 cgtcatcctt caccggtccg gcccgatcgc tcgctcgcga ccacgatttc cagctgatcg
     2941 atggcgccga actgaacgcg catgctggcg cgggaggagc acgagctgga gcaggtgcgc
     3001 tagctgcgcg tgtcgatccg agcgcgggca gacacaagag cctgtgctga gcgcagcgaa
     3061 gtatctgtcc ctacagacct caatcaacga tgcgtctagc tacttcttcc tcgctctgaa
     3121 atgtggagcc aagtcagggt ttgtttccat atgcgtgcag gcaaaaatac gcagacgttc
     3181 cgagggtcgt gggagacggc tgtgagcatg gcacgtcgcg tgcgtggtgt tctctcacac
     3241 ggatcggtca ggagaagcag ccgattcgac ggccgttatt ctcaccaagg aggtagtgat
     3301 tatgggtcga tcgattcttg catccattgc gtcggtcgcc ggcgcgttcg cgctggtggc
     3361 cgccatgctg ctcagcggcg gtttggcgac ggcgcaggac gggacgccac cgccggaatt
     3421 caccgaggac gacgtgcctc ggccggcgca catccattcc ggtacctgcg aagagctcgg
     3481 cgatgttgtg tatccactcg gtgacctgac cggtgtgacg atccagggtt ccccggtcgc
     3541 atcgccgatc gttgagaatc cggaatcgcc cgatatcgtt cagggtgagg tcgtgatcga
     3601 gagcatcacc gaggtcgaga cgaccttcga tgagctgttc ggtgccgagc atgcgatcaa
     3661 cgtgcacgac agtgccgaga acatcggtct ctacatcgcg tgcggcaata tcgagggtga
     3721 gccggaggac ggccggttgg aaatcgacat tcacccgttg aacaattccg gcttcgaggg
     3781 cattgctgtg ctcgaggaga ccgaagaggg cgtgatcacc gtcaccgtca cgctccgcga
     3841 cgtgagcgac gtcgaggcca cgccggctct ctagagatag gcaatcggct ggcaacgagg
     3901 cgaccgtggg taagcccaag gtcgcctttt ccgtgctgca gcaggtcttg gggttattgt
     3961 tcctccttgc gctgccacgg ccatcgcccg ctgatctcca gctccagctc gatgctgatg
     4021 aaggtgcgga cgagcacgat gagtgccagc accccgacgg aggtgaaggt cggatcaacc
     4081 gccacggtcc ggatgatgtc gccggcaatc agcagctcga ggccgagcag gatcgaccgg
     4141 gcaaccgcct gtctcactcg tcgaaacccg cggtgtggct catcgccgaa cgccgcaccc
     4201 gcgttgcgaa tccctgccag caggatgccg atgacaatca ccgccacacc ggtcgcctcg
     4261 accgccttcc cgacaacttc gaccgtcttc tggaattcca tgcagcgcga tctccctctc
     4321 cactcggctg gtgattctgc cgttactggc gcggtgtctc gtttcgcacg acatcggcca
     4381 cactcctacg gtgccgggct cggttcgttg gggtaggtcg gcatgccggc ggtgctgccg
     4441 ggcgggactg catgccgggc ggggcggatg ccctgcgtga ccagcgcgat gccgaccaca
     4501 accacggtga cacccagtat gtgcagccag gtgaccggct cgtcgagaaa gatggccgtg
     4561 gcgaggatgg tgccgaccgg gatcatgccc gtgagcgcac cggccgtgct cgccggaaca
     4621 tgctgcaatc cctggaacca gagggcgaag gccaccaccg tcacgatcgc ggcgaggtag
     4681 agcagcgcca gccatcctga gccggggatc tcgctgggtc gcatgtcgcc cacgtcggtc
     4741 gcagccagcg gcaggaagag caggaagccg tagacggtga cgagggtcgc catcgggacc
     4801 ggccgcagcg acggtgcggc gatcttgccc agcaccgtgt aaagcgcctt gccgacaacc
     4861 gcgagaaaga cgagcacggc gccgaggaac ggctgcgtgg cgctggaagc ctcggcggtc
     4921 ccgaacaggt tgacgatcac gatgccgagc accgccagcg ccacgccgag ccacccgccg
     4981 ggcttgaggc agtcgccgag tgcgacggac atcagcgcga tcactgccgg ggtcgagctg
     5041 gtgatgatac cgctggcgac ggcggtggtg cggcccaggc cgagcagcag cagtgcgttg
     5101 aagagcacca gcccggccag cgcctgcagt gcgacgatga ggtggagccc cgacccggga
     5161 ggggagggat ccccccggcg tggcgcacca gggcgagcag gatgatcccg gcgaggagga
     5221 accgcagggt ggacgcggtg tagatcggca tcgtgtcgac cacgatcttg ctggcgacga
     5281 ccgacgtccc cacgatgagc atcgcgctcg cgaggaagag gtagctggcg atggggatgt
     5341 cgtcgagtct gcaaacgctg cgattgctcc cgatccagtg ggttgatcat ggtaacgccg
     5401 ccattatgcg gtgtcgcatg acctcctaca atgtccgtgg gaggtcgcgt cgaggtccgg
     5461 cgcggcgagt tgaaggttgc gggagccggc aggaacgatg gagacgacaa cgcagatcgc
     5521 aattgcccgg cgcattgaca tgatcgccga gtggcgcgcc gagcgggagc atcaggacat
     5581 gctgggtctc ggtccggagg cggccaggcg cagtcgccgg tcggccgagg ggctgcggga
     5641 gctggcgaac cacatcgccg ggctgcccga cgaccatccg cacatcgtgc ggctgcggga
     5701 gctggcgttt gccggcgggc agttcgatcc cggaccgatg ctgctcaatg agctgggacg
     5761 gttccggttc cacgagccgg agcaacgcgt cgaggtcttt ctggagaaga tggtcgaata
     5821 cgccgagcag gatgccagcg agatcgcgct cttcggcggg ccgcaggttc ccggcgacaa
     5881 cccgtggatt atccgcatca tcgacgatga ggacgactgg gacgcctgac ggtgcgggag
     5941 ttgagagagc cactcccgat ttccacacat ccgactttca ccgcagccag atagcctgtc
     6001 cgagggcctt gacggcgccg cccggggcgt ggatttccgg gcggacgaag ggcaggtcgc
     6061 cgggcaggcg agagctcggc acgaaactct cgacatcgtc gccggtgatc ccctcggcgt
     6121 ggacgacgcc ctgcgggccg atcagcagca gcgcctgtcc atccgcgccg gcaatccgca
     6181 tccgaacctc actcccgtca ccgagattgt agagttgcgg gccggaaggc gactcgctca
     6241 cgaacgtccg cccctcccga accgcggcga ggaccgcctc ggcggtcagc tctccgtcga
     6301 cctcggccca tgtcgtcggc cagccgattc tcgttggctc cagcgggcgg tcgggcgcac
     6361 cgaacaggcg gtgctcgtgc atgtcgctgc cggcaaccat cggcagcggc cgctcgctgc
     6421 ccaactgtcg gtgccaccat tccagtgacg cagcgttgaa gcgtgcccac cagctgttcc
     6481 acgcctcgat cacgtggaag ccggttactt cggggaactc ccactccggc ccgaagggct
     6541 tcgggtggtt gagggagacg gtgccaccgt cgcgcagcgc cgcgtcgacg gcgccctgga
     6601 gaccctcggc ggacggatcg cggaactcgt accagtgctc ggtgccccag acgttgaagt
     6661 gcccggcgta ggtggtcacc tcgacgcccg gcaccaggac cggccactca tcaccctgcg
     6721 gcaccagtcc gacgggcgac tgcgcccggt tgtggtcggt gatgccgtag aagtggagtc
     6781 ctgcttccac ggcggtgacc gcgatctccg ccggggtagc ggtgccgtcg gagtagacgg
     6841 tatgcacgtg gaggtcgccg cgataccagt tcggctccgc gggtgcgggg aggtagggcc
     6901 gccacacgct gtcgatgtcc ggtgcatgtt tctcgtcggg cagggcgatc tccgggttca
     6961 gccagatccg cacctcccat cgcaggccgt tctgccctat cttgtaagga cccagcagca
     7021 tgtgccatgt accggcgccc ggcttaccgg cgcggtaggg cggggtggcg cgctcggtgc
     7081 cgaccacgat ccgggtctgc acgctgccac tccaccctcg gaaccccggg ctgcggcttt
     7141 cgatgccacg ctcatcaaag aggccgatgt cgagggtgtt gccgctgcga gcgaggggcg
     7201 acgagtcgat gcgatcgttg tagccaacgt cgatcagcag ctggtcgaca ctctctggca
     7261 cctcgaatgg gacgtgctcg taggcgcact ggtgctcctc gtgaaagatg ccgccgaggg
     7321 tgacttgtgg tggagtgtca ggcaggggga tgtcggtcat cgaaggtccc ttcgcgatta
     7381 cgccggcggc gctcccggtc tacagcgacc gacgccagcg tcggtttggt gtgccatgtc
     7441 gggcgagttg ccacactggc tgtggcaacc gttacgcctt tgagtgtgcc atgctcacaa
     7501 ggagtcgcaa gccgttgccg ccggtcgccc gtcacactat gctcaactgg ttccgcgtcg
     7561 ctgcggagtc gccatgccgt cgctatctac acagaacgga acccagtgtg agcagtgacc
     7621 agcaggaaca gcgtgtcgag aacgaggggc cggtcgccca gttcgtcagc gtcgatgaga
     7681 cgctgcatgc accgtccttc aacggtggca agccgcgtgt cgtcatcgtc ggtggcggct
     7741 tcgccggcct gaacgccgcc aaggcgctcg atggcgcggc ggtcgaggtg ctcctgatcg
     7801 accgtcgaaa tcaccatctc ttcgcaccgt tgctgtatca ggtggcgacc gcgcagctct
     7861 cgccggcgaa catcgcccaa ccgatccgcg gcattctgcg ccggcagaag aacctttcgg
     7921 tgctgctcgc cgaggtggtc ggcgtcgagg tgaatggtcg ccgggtccgt ctcgacgatg
     7981 gccggacggt cgattacgac tacctgatcc tggccgccgg cgcgacgcat tcctatttcg
     8041 gcaacgatca gtgggcgccg ctcgcgcccg gtctcaagac gatcgaggat gcgcttacga
     8101 tccgccgccg tatcctgctg gccttcgagg acgccgagcg ggagccagat ccggcgcgac
     8161 gcgcgagcat gctcaccttt gtgatcgtcg gtggtggacc gaccggtgtc gagctggctg
     8221 gcgcgatcgg cgagctggcc aaccacacgc tggctcgcga tttccagcat atcgatcccc
     8281 gcgacgccca cattctgctg ctggagggac tgccgcagat cctgccgatg ttcaacgaaa
     8341 agctggctcg gcaggccgtc gaggatctgg agcggttcgg tgtcgaggtt cgcaccaact
     8401 cactggtcac cgcgatcgat ccggagggcg ttaacgtcgg agacgagcgc atccccacgc
     8461 acaatgtctt ctgggccgcg ggggtgagtg ccgcgccggt gacacggtcg cttgccggtg
     8521 tcgcggagct cgaccgagcc gggcgtgcca cggtgacgcg tgagttgaac attcccgggc
     8581 acccggagat cttcgtgatc ggagatgctg ccagcgcacc gggcgagaac ggcaagccgc
     8641 tgccgggtgt cgcgccggtg gcgatccagg agggacagtg ggtcgcgtgc aacatgcgcg
     8701 ctgcgcttga ggggaatgcc tacgcgccgt tccggtaccg ggatcagggt agcatggcga
     8761 ccatcggccg caaccgggcg atcgccgatg tcaagggctt ggagttcacc gggttcgtcg
     8821 catggctcgc gtgggcgctg gtgcacgtgg cccagcttat cgggttccgg aaccggctga
     8881 tggtcggcgc acagtggctc ttcaactact tcacctaccg gcgcggcgct cgactgatca
     8941 ccggcgagct gagtgacgag cgacggaagg acatgatggc ccggctcggc aagcgtagtg
     9001 gtgagctgcc ggctcgctga gcgatagtac cagcaggctg acgagcggcc ggatcggtcg
     9061 cgaagattta ccagtcggtg agcgaacagg ggcggaaccc atgtgggtag ccgcccctgc
     9121 tgagggcaac gggctctacg ggaagctgtt cgcgtcgagc gggtcggttc cggcgaccac
     9181 ttcatcgtag tcggtccgcc cgtcgccgtc ggtgtcaaca gccgtggggc tggtctccga
     9241 gaagtagacc tcgtcaccgt ctgtcaggcc gtcgacatcg gtgtcgacga ccagcgggtc
     9301 agtgttgtag agcagctcgc cgccgtcgta gagcacgtcg ccgtctgtat ccgagttgag
     9361 cgggtcggtt ccggcagaaa acacctcgtc gccgtcaagg atgccgtcct catccgtgtc
     9421 cgcgctgagt gggtcagtgc cgagctcgat ttccactccg tcgaagaggc cgtcgtcgtc
     9481 ggtgtccgga tcatccgggt tggtgccggc tgcgatctcg tcggcgttgt cgacaccgtc
     9541 accgtccgag tcgatgtcct gtggctcctc cgccacaggc gtggccgggg aaggcgtggc
     9601 caccggcgtt gccggtaccg gagtggctgt cggttccggc gtcgcctcgg gcgtttctag
     9661 ctcctgggac gctaccggcg tcgctggttc ctcgatcgac tcgatcgcag ggccgatgcg
     9721 ggcgacgacc acggcagctt catccgaggt ggcggtgatg gtcagctcac cactggcgag
     9781 acggtccccg ccggcggtca tggagaactg ctcgtcctcg gcctgcacgt cgacctgacc
     9841 actgcggacc acaatgagcg actgcccgtt acccatcggc acggtggagc tttcgccctc
     9901 gtcgaggcgg atacgaatca ggtcgacgtc gtagctgccg ccctccacct ggaacggcac
     9961 gctgatccgc cctggcgagc cgctgagcga tgcctcatca accggcatca cgtctaccgc
    10021 cagcacgctt tgcggcggtc ccatgctggt gacgctgctc tcgttgcccg gcaccagtac
    10081 ggctccctcg ccggatgcga cccgctgccg tagaccggtc gagagatcct cgacgaggac
    10141 cggcacgcca tcggtcgtga gcagcgccgg tacgtcgccg ctgatcgggg cggtgtcgcc
    10201 gtcgatctgg agattacgga tgcgccagac cgtttcgccc gcgggcagct cgacgaccgc
    10261 gtgagcgacg acggcagcgt tgtcctctgc gggggagggg cccgcggggc cgagagcctg
    10321 ggccagcacc gtggcgacac cgatgaccgc gatcagggcg ccgctgatga tggcagtggc
    10381 ccagcggccg tggacgccgg attggttcat gtgtcgatcc ttccaagggg tcacatcgat
    10441 cggtcggaac atcgcagtgg cagagtacgg actcgacgta cagcacgtca aggaatggac
    10501 acacgaacac caccgccgga acactccgac ggtggtgtct cagttcgtct ggagctgcct
    10561 ggccgggggt tagccgcgca ccttgcggta gttgttggtg acggcatccc agttgacggt
    10621 gttccaccag gcgttcaggt agtccgggcg cttgttctgg tacttgaggt agtacgcgtg
    10681 ctcccagaca tccacgccga ggatcggcgt gcccgacccg tccatcaggg ggttgtcctg
    10741 gttcggggtg ctggtcaccg ccagcttgcc ggtaccatcg accaccagcc aggcccaacc
    10801 ggacccgaaa cggttcgcgc cggcggtatt catctcctcc ttgagcgcgt caaacgagcc
    10861 gaacgcctcg ttgatggtgt ccgccagcgc gccggagggc tggccacccc cattcggacc
    10921 catgacggtc cagaacagcg agtggttgta gtgaccgccg ccgttgttgc ggaccgctgc
    10981 gcgcttcgct tccggcacac gatcgaggtt cttcatgagc tcctcgatgc tgagcccctc
    11041 aagatcagtg cccgcgatgg cgtcattgac cttctgaaca taggtgccat ggtggcgatc
    11101 gtggtggatt tccatagtct gcgcgtcgat gtgtggctcg agtgcgtcga aggcgtacgg
    11161 cagggcagga agttcgtaag ccattggctg ttcctccatc ggttacgtcg tgcgtgcccc
    11221 catattcggc gcatgccgcc acaggggcgc ccctgagtac cggggccatc ccaagtcgtc
    11281 gcaagacgcg ggccacccgg aggtcacttc tggccgccga acggctcgtt cgtgccgtca
    11341 gacggctacc ccggctcctg gcggtactcg tcgtctgaga cgtgttcccc cagttcacgc
    11401 tcacgccctc gtcgttgact tcctgcatcg cgatatgtgc catgaaccgg tccggggcgg
    11461 agccgtgcca gtgctc
//
LOCUS       DASYWL010000097         7281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_360853, whole genome shotgun sequence.
ACCESSION   DASYWL010000097 DASYWL010000000
VERSION     DASYWL010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7281
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360853"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..750
                     /locus_tag="VGR22_03635"
     CDS             43..750
                     /locus_tag="VGR22_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEV2127694.1"
                     /translation="MFYFDPLYFVFMLPGLIFMLWAQSKVKGNYTKFSQVRNSANVTG
                     AQAARRVLDSHGLYDVQIEQVPGELSDHYDPRDRTLRLSQGVYGVPSIAAIGIAAHEA
                     GHAIQHANSYGPLKFRTALVPAVTIGSNLGFIVLLAGFFMGIVGLAWAGVALFALSTL
                     FALVTLPVEFDATKRAKAALVNVGLVDYGRRTGEESQGVAKVLDAAAWTYIAGFASSV
                     LTLLYYVMLVSGMRRSE"
     gene            complement(848..1216)
                     /locus_tag="VGR22_03640"
     CDS             complement(848..1216)
                     /locus_tag="VGR22_03640"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV2127695.1"
                     /translation="MGARIEHEATRVDRSPSGLAIRHLVTGEVGSTDLYTGEQWLERG
                     EQVLLHTHPVEEVLIFTDGSGEATLDGNIVPVAGGVTLHIPAGEVHGFRNTGDGRLHV
                     FVIFPGNTFARTDMIEDGSG"
     gene            complement(1216..2121)
                     /locus_tag="VGR22_03645"
     CDS             complement(1216..2121)
                     /locus_tag="VGR22_03645"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEV2127696.1"
                     /translation="MSTRNTSPRSIRLGAVVPQTELPPDAGAARHWFAQVEQLRFDHA
                     LIYDHVLGADTSVRPGWSGAYEATDPFLEVMVALAWAAGFTERLELATGVVILPQRQT
                     ALVAKQAATIDHLSGGRLRLGVGIGWNKVEYDALNEQFNNRGKRLEEQIALMRRLWTE
                     PVVTFEGEWHHVDHAGILPLPVQRPIPVWIGASADVAIRRAARLADGFYPQGVPSEKM
                     EHMLAVLFEALERHERDRETFGIEARITVSTDDEAEWRRQAAWWREKGITHLSLNTMR
                     GGLTSIEAHLEAFSRGREALLQEIG"
     gene            2332..3951
                     /locus_tag="VGR22_03650"
     CDS             2332..3951
                     /locus_tag="VGR22_03650"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEV2127697.1"
                     /translation="MASDSNRVRMIDRRRLIRGLAATSAFAWVPGARAQEAPPVIEPI
                     DGDPVQQDPIEPESDPSAVIETFEEAEGELRYFAETGHNLDEPFLSAWLAAGGEAVLG
                     RPLSEERFVPDTGVVEQYFEGIVLVNAPNSAGQWVTTGAPLGDVRAAAAAPSSARSTV
                     SGCSGAATCEFFIETGHTVSGEMAGFWYQYGGASVFGLPVSEPFERDGATVQVFQNAI
                     LRIAGGTVTTEPIVRDLIADLGLENDAAFLPAPPTLGTTTLVRASDGLRLRGGPSTDA
                     EIIAVLEDNAEFIAVTGATGAWVPGYADGYSGYVAAEYLAEPEELPSFVGGQWNPSVW
                     QGIALSETNIRTEPTTASRIVTTVPYGELVRVTDWVKGEQVDGGTDTWAMLDDGTYMY
                     ERNVGRRGPVEPTPVPGDAPWEGKWIDCNLTQQLVIAYEGRTPVRVAVTTTGKPGWET
                     PVGFFAINTRVENETMESGAIGAEEFYRLENVLYTQYFTDRGHALHYAWWKTPETIGR
                     PGSHGCLNLLLDEAQFFWGWATIGTPVYCHY"
     gene            4219..5409
                     /locus_tag="VGR22_03655"
     CDS             4219..5409
                     /locus_tag="VGR22_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127698.1"
                     /translation="MAGRPKPFSSGWMIGPPPEPEPPRLFPGRVRSTVAAIGRRLPGE
                     RVLAIWVVLALVLGSVVFGNPPGRAEPDRLAMLEVGGTPTPMIDWNAQESTASSASIS
                     TDELDMTDEAEVEETVQPTPPELHLEIAPPTEEVSLTPDGLLPEYRILTYYGFPNNEK
                     MGILGEHDTERLLDLLREQAAEFEAADPSRPVLVGMEVIASVAQREPQADGSYLLDTP
                     SELLNRYSDFAEANDILLFLDVQIGRRTVQAEVEGLRPWLEKPHVHLALDPEFAMREG
                     EIPGEHIGQVDASDITWTQEYLADLAAEVGIPPKVLIVHQFHYTMIENKDAVSPVAGV
                     QLVIDADGWGTPAEKRATYDVVIAQQPIEFHGLKLFYRQDVPLMTAEEILDLEPAPDL
                     IIYQ"
     gene            5476..5925
                     /locus_tag="VGR22_03660"
     CDS             5476..5925
                     /locus_tag="VGR22_03660"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEV2127699.1"
                     /translation="MGRIFVLDQEGIDALLRSALVGRIACCAHDDDRPYLVPIPFGYD
                     GESIYSISGPGRKLDIMRNQPSVAFEVDVAEAEDRWQSVVADGEFEELTHPKERLRAI
                     EIVNPAAGIDAVSPDAIVYRIRLTRLSGRFEVPDAEASRYENAMARR"
     gene            6287..>7281
                     /locus_tag="VGR22_03665"
     CDS             6287..>7281
                     /locus_tag="VGR22_03665"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV2127700.1"
                     /translation="MKRYGYNPNPVNKLYRDALHGRLSRREVMKRGTALGLSSALMGT
                     ILRAESLGAQGTPDDRQRGWSIPSAEELQEELQLSTDYAGVQFNYVGGSEGPNTPFEQ
                     ALFERFNEITGAQATLVQGAESATDRLQFYLQTFAAQAGDIDAAQIDVIWPGILSSHA
                     VDLSDNMPDGDFFERIVENNTVNDILVGIPMFTDAGILYSRTDLLEEYGFEGPPETWD
                     QLEEMAQTIMEGEITETPEFTGFVWQGNAYEGLTCDALEWQYSHEGGRIIDPQTLEVE
                     LNNESAITAFERAAGWVGNISPEAVTGYMEEDARAVWQGGSAAFMRNWPYAYSLG"
BASE COUNT         1294 a         2279 c         2467 g         1241 t
ORIGIN      
        1 gccagtgcgt cgcgggtatc ggaaaggtag ccaggaaagc agatgttcta cttcgatcct
       61 ttgtacttcg tgttcatgct gcccgggctg atcttcatgc tctgggcgca gagcaaggtc
      121 aagggcaatt acaccaagtt ctcgcaggtc cggaacagcg ccaatgtgac gggggcgcag
      181 gcggcccgcc gagtgctgga cagccacgga ctttacgatg tccagatcga gcaggtgccg
      241 ggcgagctga gcgatcatta cgatccacgc gaccgcacgc tgcggctctc gcagggcgtc
      301 tacggcgtgc cgtcgatcgc ggcgatcggc atcgcggccc acgaggcggg gcatgccatc
      361 cagcatgcca actcctatgg gccgttgaag ttccgaacag cgctggtgcc ggcggtgacg
      421 atcgggtcca acctcggctt cattgtgctg ctcgccgggt tcttcatggg catcgtcggg
      481 ctggcgtggg ccggtgtggc gctcttcgcg ctctcgaccc tcttcgcgct ggtgacgctg
      541 cccgtggagt tcgacgccac gaagcgcgcc aaggcggcgc tggtcaatgt cgggctcgtg
      601 gactacggcc gacgcacggg cgaggagagc caaggcgtcg ccaaggtgct tgatgccgcg
      661 gcgtggacgt acatcgcagg cttcgcgtcc tcggtgctga cactgctcta ctacgtgatg
      721 ctcgtgagcg gcatgcgccg cagcgagtag cagtctgaaa cacgacggga gaaaatcacc
      781 ccggcgggcg aacccggccg gggtggtgtc gttgtcggag cactgacagc gaagccaagc
      841 ctccatgtca tccggagccg tcttcaatca tgtccgtgcg ggcgaaggtg ttgccgggga
      901 agatgacgaa gacgtgcagc cgtccgtcgc cggtgtttcg gaagccgtgc acctcaccgg
      961 cgggaatgtg cagcgtcacg ccgcccgcga ccggcacgat attgccgtca agcgttgcct
     1021 cgccggagcc gtctgtgaag atcagcactt cctcgaccgg atgggtgtgg agcagcacct
     1081 gctcgcctcg ctccagccac tgctccccgg tgtagaggtc ggtcgagccg acctcaccgg
     1141 tcaccagatg ccggatcgcc agaccactcg gactgcgatc gacccgtgtc gcctcgtgct
     1201 caatgcgggc gcccatcagc cgatctcctg gaggagcgcc tctcgcccgc gcgagaaagc
     1261 ctcgagatgg gcctcgatgc tggtcaaacc gccgcgcatc gtgttcagcg acaggtgggt
     1321 gatccctttc tcgcgccacc aggccgcctg acgccgccac tcggcttcgt cgtccgtcga
     1381 gacagtgatg cgcgcctcga tgccgaacgt ctcgcggtcg cgctcgtggc gctcaagcgc
     1441 ttcgaacagc acggcgagca tgtgttccat cttctcgctg ggcacgcctt gggggtagaa
     1501 gccatcggcc aggcgcgccg cgcgcctgat ggcgacatca gcgctggcgc cgatccagac
     1561 cgggatcggt cgttgcaccg gcagcggcag gataccggcg tggtcgacgt ggtgccactc
     1621 gccctcgaag gtgacgacag gctctgtcca gaggcgccgc atcagggcaa tctgctcctc
     1681 cagtcgcttg ccgcggttgt tgaattgttc gttgagcgcg tcgtactcga ccttgttcca
     1741 gccgatgccg acaccgagcc gtaggcggcc gcctgagaga tggtcgatgg tggccgcctg
     1801 cttggcgacg agcgccgtct gccgctgcgg caggatcacg acaccggtcg ccagctccag
     1861 gcgctcggtg aagcccgccg cccacgcgag agcgaccatc acctcgagaa aggggtccgt
     1921 agcctcgtag gcgccgctcc agcccggtct gacgctggtg tccgccccaa ggacgtggtc
     1981 atagatgagc gcatggtcga atctgagctg ctcgacctgc gcgaaccagt ggcgagccgc
     2041 gccggcgtcg ggtgggagct cggtctgggg caccaccgcg ccgaggcgga tcgagcgggg
     2101 ggatgtgttg cgtgtggaca tgccgagaac accttttcgg gtagtcgtgc gtagagacag
     2161 gcagggctgc cacgatgtgg ctcccgcgtt ccgtcgaagc ttgcgatcag gacgggaccg
     2221 cgtcaagacg aaatcaggaa actctgtcaa gatagtacgg acggcgcggc atcgtgggga
     2281 tgcctgcggt agtttgtgcc ggactgcaac gataaccagg agccgacgaa gatggcgagt
     2341 gacagcaaca gggtgcggat gatcgaccgg cggcggctca tacgtgggct ggccgcgaca
     2401 agcgcgttcg cgtgggtgcc aggggcacgg gcgcaggagg ccccgcccgt gatcgagccg
     2461 atagacggtg atccggtaca gcaggacccg atcgaacccg agtcggatcc ctcagcggtc
     2521 atcgagacat ttgaggaggc ggagggggag ctgcgctatt tcgctgagac cggccacaac
     2581 ctggatgagc cgttcctgtc cgcctggctc gctgccggcg gcgaggccgt actcggtcga
     2641 ccgctctccg aggagcgctt cgtgccggac accggagttg tggagcagta cttcgagggc
     2701 atcgtgctgg tcaacgcacc caacagtgcc ggacagtggg tcacgaccgg tgccccgctc
     2761 ggtgatgttc gcgccgccgc agccgcgccg agctctgccc gatccaccgt ctccggctgc
     2821 tccggcgcgg cgacatgtga gttctttatc gagacggggc acaccgtgtc cggcgagatg
     2881 gcaggcttct ggtatcagta cgggggcgca agcgtcttcg gcctgccggt ttccgagccc
     2941 ttcgagcgag atggcgcgac ggtgcaggtc ttccagaacg ccatcctgcg gatcgcgggt
     3001 ggcaccgtga cgaccgagcc gatcgtgcgc gacctgattg ccgatctggg gctggagaac
     3061 gacgctgcct ttcttccggc acccccgacg ctcgggacga ccacactggt gcgcgccagc
     3121 gacggcctgc gcctgcgtgg cgggccgtcg accgacgccg aaatcatcgc cgttctggag
     3181 gacaacgccg agttcatcgc cgtcaccggg gccacgggcg cttgggtccc ggggtacgcc
     3241 gatggctact cgggctacgt cgccgccgag tacttggccg agccggagga gttgccgtcc
     3301 ttcgtcggcg gtcagtggaa tccgtccgtt tggcagggaa tcgcgctctc cgaaaccaat
     3361 attcgcaccg agccgacgac cgcgagccgg atcgtgacga ccgtgcccta cggcgagctg
     3421 gtgcgcgtga cggactgggt gaagggcgag caggtcgacg gcggcaccga cacatgggcg
     3481 atgctggacg acggcaccta catgtacgaa cgcaatgtgg gccggagagg tccggttgag
     3541 ccgacgcccg tgcccggtga cgcgccgtgg gaggggaaat ggatcgactg taacctgacc
     3601 cagcagctgg tcatcgccta cgaggggcgt acgccggtcc gagtcgcggt caccacgacc
     3661 ggcaagcccg gctgggagac gccggtgggg ttcttcgcca tcaatacccg tgtcgagaat
     3721 gagacgatgg agagtggcgc gatcggggca gaggagttct accggctcga gaacgtactc
     3781 tacactcagt acttcacgga tcgagggcac gcactgcact acgcatggtg gaaaacgccg
     3841 gagacgatcg gccggccggg cagccacggc tgcctcaacc ttctgctgga cgaggcacag
     3901 ttcttctggg ggtgggcgac catcggcacg ccggtctact gccactacta ggttagtctg
     3961 accagcgcgc agaggcaggg gtggcctggt gtagccgcca gtgcgtcgga aacgatgcgc
     4021 cgccgccctg gctgcacctc gtcagggaat cgctgccctg ttcgcaccac ttgtaggcac
     4081 gctgtactgg caccatgggc gcaatcgcgt accataggtg cgttatgacg atgtcggcct
     4141 gtggccgaat gctcttgcgt gcgtcagcgc cgcggcaggg cccattgccc tgtgcgaggg
     4201 gctcgctgga ggttttgcat ggccggaagg ccgaaaccat tctcgtctgg ctggatgatc
     4261 gggccaccac cggagcccga gccacctcgg ctctttcccg gtcgggttcg ctccaccgtc
     4321 gcagcgatcg gccggcgtct gcccggagaa cgggtccttg cgatatgggt ggttctggcg
     4381 ctggtgctcg ggtcggtcgt cttcggcaac ccgccgggcc gggccgaacc cgatcgactc
     4441 gccatgctcg aggtgggtgg caccccgacg ccgatgatcg actggaacgc ccaggagtcg
     4501 acagccagtt cggcgtcgat ctcgaccgac gagctagaca tgacggatga ggccgaggtg
     4561 gaggagaccg tgcagcccac accacccgag ctgcacctcg agatcgcccc gccaactgaa
     4621 gaggtgagtc tgacccctga tggattgctg ccggagtacc ggatcctcac atactatggc
     4681 tttcctaata acgaaaagat gggaatcctg ggtgagcatg acacggagcg cctgcttgat
     4741 ctgctgcggg agcaggctgc cgagttcgag gcggccgacc cgtcgcgccc ggtgctggtc
     4801 gggatggagg tgatcgcgtc ggttgcccag cgcgagccgc aggccgacgg cagctacctg
     4861 ctcgatacac cgagcgagct gctgaaccga tattccgact tcgccgaagc caacgacatc
     4921 ctgctcttcc tggatgttca gatcggccgc cggaccgtgc aggcggaggt ggaggggttg
     4981 cggccctggc tggagaagcc gcatgtccac ctggcgctcg atccggagtt cgcgatgcgg
     5041 gaaggcgaga tcccgggcga gcatatcggg caagtcgacg caagcgacat cacttggacg
     5101 caggagtacc tcgccgacct cgcggcggag gtcggtatcc cgccgaaggt gttgatcgtg
     5161 caccagttcc attacacgat gatcgagaac aaggacgcgg tctcgccggt cgccggtgtg
     5221 cagctggtga ttgacgccga cggctgggga acaccagccg agaaaagggc cacgtacgac
     5281 gttgtgatcg cccagcagcc gattgagttc cacgggctca agctcttcta ccggcaggat
     5341 gtgccgctga tgaccgccga agagattctt gacctcgagc cggcgcctga cctcatcatc
     5401 tatcagtaga cgcacgacga tgacggcgca tgccggtcgc agcaccgcgc cgggaattgt
     5461 ggagtgaatc ggaccatggg gagaatattt gtcctcgatc aggaggggat cgacgcattg
     5521 ctgcgctcgg cgctcgtcgg gcgcatcgcg tgttgtgcgc acgatgacga ccgcccctac
     5581 ctggtgccga tcccgttcgg ttacgacggc gagagcatct actcgatcag tgggcccggg
     5641 cgaaagctcg acatcatgcg aaaccagccg tcggttgcct tcgaggtcga cgtggcggag
     5701 gccgaggatc gctggcagtc ggtcgttgcc gacggcgagt tcgaggagct cacgcacccg
     5761 aaggagcggc tccgggcgat cgagatcgtc aatccggcag cgggtatcga cgctgtctcg
     5821 ccggacgcga tcgtctaccg catccggctg actcggctgt ccggccggtt tgaggtgccc
     5881 gatgccgagg cttcccggta cgagaacgcg atggcccgtc gctgatgaaa catgtcagca
     5941 gacaacacga agatccgggg aaagtcgtgg caaagcgaca gtgaccaacg agatccttcg
     6001 cacgtcgtgg ccccgatctt gctccaacag gaaatcgagg ctgttccgca acggagagga
     6061 gtagcgcgga caatcggccg agtcagtgac gacttgactt ttcgcgttac accggagtag
     6121 aacagcgata cattggttca tggacgagac gacttggcgg cagggtgctg ccaggacctg
     6181 atggcccaac tttggcaagg catccgcagc ccgcgagacc ctcaccgtcc gaaccgcacg
     6241 accgacacgg gcgttcccag aggaacgccg acaacaggag gttcccgtga agcgatacgg
     6301 ttacaacccg aacccggtca acaagctcta ccgcgacgcc ctgcatggtc gtctgtcacg
     6361 acgcgaagtc atgaagcgcg gtaccgcgct gggcctcagc tccgcgctga tgggcacgat
     6421 cctccgagcc gagtcgctcg gcgcgcaggg gactcccgat gatcgccaga ggggctggtc
     6481 cattcccagt gccgaggaac tccaggagga gctgcagctg agcaccgact atgccggtgt
     6541 gcagttcaac tacgtcggtg gctctgaagg tccgaacacg ccattcgagc aggcgctgtt
     6601 cgagcgcttc aatgagatca ccggcgctca ggcgacgctg gttcaaggcg cggagtctgc
     6661 gaccgaccgg ctgcagttct atcttcagac cttcgccgcc caggcgggcg acatcgacgc
     6721 agcccagatc gacgtgatct ggccgggtat cctctcgtcg cacgcggttg acctgagcga
     6781 caacatgccc gacggcgact tcttcgaacg cattgtcgag aacaatacgg tcaacgacat
     6841 tctcgtcggc atcccgatgt tcacggacgc cggcatcctc tactcgcgca ccgatctcct
     6901 tgaggagtac ggcttcgagg ggccgcccga gacgtgggac cagctcgagg agatggcgca
     6961 gaccatcatg gagggcgaga tcaccgagac gcccgagttc actggtttcg tctggcaggg
     7021 caacgcctac gaggggctca cctgcgacgc gctcgagtgg cagtactcgc acgagggtgg
     7081 ccggatcatc gacccgcaaa cccttgaggt ggagctcaat aacgagagcg ccatcaccgc
     7141 attcgagcgg gctgccgggt gggtcggaaa catctcgccg gaggccgtca ccgggtacat
     7201 ggaagaggac gcccgcgccg tctggcaggg tggcagcgct gccttcatgc gcaactggcc
     7261 gtacgcgtac tcgctcggtc a
//
LOCUS       DASYWL010000098         3991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_323354, whole genome shotgun sequence.
ACCESSION   DASYWL010000098 DASYWL010000000
VERSION     DASYWL010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323354"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /gene="ruvB"
                     /locus_tag="VGR22_03670"
     CDS             complement(<1..538)
                     /gene="ruvB"
                     /locus_tag="VGR22_03670"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEV2127701.1"
                     /translation="MSEQTQDTRMMSGQNREEDSRLDRSLRPRSLDEYIGQDRVKNNL
                     KIFIQAARAREEALDHILLYGPPGLGKTTLSSIIATEMGVSLRVTSGPAIERQGDLVS
                     ILTNLKQGDVLFIDEIHRLNRVVEEVLYPAMEDYAVDIVIGKGPSARAMRINLPRFTL
                     VGATTRLALMTGPLRDRFG"
     gene            complement(610..1320)
                     /locus_tag="VGR22_03675"
     CDS             complement(610..1320)
                     /locus_tag="VGR22_03675"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2127702.1"
                     /translation="MPRSRPVRRPPDRKRSAPRKPVSEQFGTSRAMKIGFIALAMLFT
                     CSLLAGSLVLIPFDELFGSSDDAAQNIVDRNDDIIEELRATYEANPDDVGNVILLANV
                     LGNSGRLDEAIPYYEEAIDLAPDDPSVRLDFARSLADGGYHADAELQFERVLEMEPNS
                     QEALYYLAELYMDWEPPREEEARELYQRAIEADPDSFLAEQARNQLDSMFGTPTSPAS
                     PQATPAGDGTVGRYEQAF"
     gene            1452..1526
                     /locus_tag="VGR22_03680"
     tRNA            1452..1526
                     /locus_tag="VGR22_03680"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1484..1486,aa:Thr,seq:cgt)
     gene            1551..1637
                     /locus_tag="VGR22_03685"
     tRNA            1551..1637
                     /locus_tag="VGR22_03685"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1585..1587,aa:Leu,seq:cag)
     gene            2063..2136
                     /locus_tag="VGR22_03690"
     tRNA            2063..2136
                     /locus_tag="VGR22_03690"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2097..2099,aa:His,seq:gtg)
     gene            complement(2370..2660)
                     /locus_tag="VGR22_03695"
     CDS             complement(2370..2660)
                     /locus_tag="VGR22_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127703.1"
                     /translation="MDWHGDTLTLEYRTRGRGDAEWTPRTLRVWLEWTPCHFGGERVW
                     FRCPGCLSRRAVHFSVGGVFRCRACHDLAYTSTREDAIERAHTGASSRCNAG"
     gene            2727..2942
                     /locus_tag="VGR22_03700"
                     /pseudo
     CDS             2727..2942
                     /locus_tag="VGR22_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012890904.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(3064..3699)
                     /locus_tag="VGR22_03705"
     CDS             complement(3064..3699)
                     /locus_tag="VGR22_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127704.1"
                     /translation="MSTTTLRSDPEYTAELLERQQALQSQAQAVIASLDLITLFTPLG
                     RPILVGSVITGLMVWPDIDIVVPCASLDPDRLWDTVRPLASHPRIKQLRWSNVRGPFN
                     STGRPEIDGYYFCVHYEEGNINAEEEWRIDCWFVPLDAPRPEFALMDRLQQELTEETR
                     LAVLSIKDVWSRHPDYLSHKVCSTDIYNAVLDSNIRSPDEFATWLRERKIR"
BASE COUNT          718 a         1268 c         1284 g          721 t
ORIGIN      
        1 cgccgaagcg gtcgcggagc gggccggtca tcagtgccag gcgggtagta gcgccgacca
       61 gcgtgaagcg cggcaggttg atccgcatcg cgcgggccga gggtcccttg ccgatcacga
      121 tgtcgacggc gtagtcctcc atcgctgggt agagcacctc ttccacgacg cgattgagcc
      181 ggtggatctc gtcgatgaag aggacatctc cctgcttgag gttggtaagg atcgagacga
      241 ggtcgccctg ccgctcgatc gccgggccgg aggtgacgcg gaggctgaca cccatctctg
      301 tcgcgatgat cgaggagagc gtggtcttgc cgagccccgg cggcccgtag agcaggatgt
      361 ggtcgagcgc ctcctcccgc gcccgggccg cctggatgaa gatcttcagg ttgttcttca
      421 cccgatcctg cccaatgtac tcatcgaggc tgcgcgggcg cagcgaccgg tcgagccggg
      481 aatcctcctc ccggttctgg ccgctcatca ttcgcgtgtc ctgcgtctgc tccgacatca
      541 cctgccgacc ttcactgctc ccgtcagggg gtcgccgcgc cccgccattc tcccggaagt
      601 ataacgcgat tagaacgcct gttcgtatcg cccgaccgtc ccgtcgccgg cgggcgtggc
      661 ttgcggcgag gccggtgagg ttggtgtccc gaacatcgaa tcgagctggt tccgcgcctg
      721 ctcggcgagg aaggagtcgg gatcggcctc aatggcgcgc tggtagagct ctcgcgcctc
      781 ctcttcgcgg ggcggctccc aatccatgta gagctccgcc aggtagtaga gcgcctcctg
      841 gctgttcggc tccatctcga gcacgcgctc gaactgaagc tcggcgtcgg cgtggtagcc
      901 accgtcggcc agcgaccggg cgaagtcgag tcgcaccgag ggatcgtccg gcgcgaggtc
      961 gatcgcctcc tcgtagtacg ggatcgcctc gtcgagccgg ccggagttgc cgagcacgtt
     1021 cgcaagcagg atcacgttgc cgacatcgtc cggattggcc tcgtacgtgg ccctcagctc
     1081 ctcgatgatg tcatcgttcc ggtcgacgat gttctgggcg gcgtcgtcgc tcgagccgaa
     1141 cagctcgtcg aacggaatga gcacgagcga cccggcgagc agcgagcacg tgaatagcat
     1201 ggccagcgcg atgaagccga tcttcatggc tcggctggtg ccgaattgct ccgacacggg
     1261 ctttctgggt gccgagcgct tgcgatcggg cgggcgacgg acgggtcggg aacggggcaa
     1321 gctggtcact cctgtcggtg gctgggaggg ggcggcaggg ctgccaaagg caacgttacc
     1381 acccggcgcc gcacttggcg gggtccctcg cctttgctat actacctaac gctgcgccgc
     1441 gggaggcgga cgccgaccta gctcagtggt agagcagctg tttcgtaaac agcaggtcgt
     1501 gggttcgaat cccacggtcg gctccacacc ttggtggagc atccgcacgc gccgaagtgg
     1561 cggaatcggc agacgcgcta cgttcagggc gtagtggggg tgaccccgtg ggagttcaaa
     1621 tctccccttc ggcaccatgg aaacgcacga ccgacccgac ggacaaaggt ctgagggatg
     1681 ggtctcgcac gtttacatga gcagagatct tcggaagtcc ggggccatgg tcgctggccc
     1741 gacgagggcc agaggaaagt ccgggctgca cagagcgagg gtgcctggtg aaagccaggg
     1801 cggcgggaca ccgttccgtc gacagtccag atcggcacgc gccagctggc gcgtgagagc
     1861 aacagagacc agaactgcgc aagcagggtg aaaagggcaa tcctccccgc agcaatctcc
     1921 gattcggccg ttaccaggtt gctcgccgag gccggaagtc gagagccgtc accgaggcga
     1981 cgaggtgacc gacacttgtc gagagagatg accatcccag aacagaaccc ggcttacccc
     2041 ctttcttccg tcgatccgca tggtgcctat agctcaatcg gttagagcgc ttgtctgtgg
     2101 aacaagaggt tacgggttca agtcccgtta ggcacccgac aaaaagcccg acgttccggg
     2161 cacaacggcc acccgcacgg gtggccgttg tcatatccgg tgaccagtgg ctaccaaatg
     2221 gctaccatcc catcggcagg ctgcgcccca tagcgcatcc cgccgatggt gctctatccg
     2281 tagctgccac accaagcgct cgtatgtccg cctgtgcatg cccttcggtc ttgccggcac
     2341 gtggaacagg tcgcagcccg gcgaggcttt cagccggcgt tgcagcgtga cgatgcgccg
     2401 gtgtgcgccc gctcgatcgc atcctcccga gtcgaggtat acgccagatc gtgacaggcc
     2461 cggcaacgga acacgccccc gacgctgaag tgcaccgccc gccggctcag gcagccgggg
     2521 caccggaacc acacccgttc cccgccgaaa tggcacgggg tccattccag ccatacccgc
     2581 agcgtgcgcg gtgtccactc ggcatcccct cgcccgcgcg tgcggtactc gagggtgagg
     2641 gtgtcgccgt gccagtccat gcgcgtgacg atccattggt cgtcgcccca cgccaccgaa
     2701 cacatcgcgc cgggtcgcag cgcgccgccc cgctgcaggc gggcacgcgc tgggtcgtcg
     2761 agcgcaccca atcgtggatg agtagattcg gcaagctgcg tcgctgcacc gagaagacca
     2821 gccggacggt cgacttctac ctgttcctgg ccgccgcagt tgtcgcgacg cgctgtctga
     2881 tccagcgcgc ccgcctccgc taccggtggc ccggccgtcc caccacccgg cggctcaagt
     2941 agtgcccatt gccgggcgcc ctaagacgtg gaagatctac ttgccgccgg ggggtggtgt
     3001 cgatctgctc agtcagcgag cgaaagccga tgccccgcgc gcagcgtcgt caccgtggcg
     3061 tcctcatcta atcttgcgct ctcgcaacca ggtagcaaac tcatctggtg aacggatatt
     3121 ggagtcgaga acggcgttgt agatgtcagt gctgcagact ttatggctga ggtaatcagg
     3181 gtgtcgggac cagacgtcct tgatcgaaag gactgctagg cgggtttcct cagtcaactc
     3241 ctgctgcaag cgatccatga gagcgaactc tgggcgtgga gcgtctaacg gcacgaacca
     3301 gcagtctatc cgccactctt cttcagcgtt gatattccct tcctcgtagt gaacgcagaa
     3361 gtagtaaccg tcgatctccg ggcggcctgt gctattgaat ggtccccgca cattcgacca
     3421 gcgtagctgc ttaatgcgtg gatgggaggc tagcggacga accgtgtccc agagccgatc
     3481 cgggtcgagg ctcgcacaag ggactacgat gtcgatgtcg ggccagacca tgagtcccgt
     3541 tattacgctc ccaacgagga tcggccggcc aagaggcgta aaaagcgtta tcaggtcaag
     3601 gctggcgatg acagcctgag cctgtgactg caatgcttgc tgtctctcca atagttccgc
     3661 cgtgtattca ggatcggatc tgagggtcgt ggtactcacg cgcgtcctcc gaagcagcta
     3721 tgatcggatt ccgccagcct atcattccct tgcgccatgg agtactgttg gagactccat
     3781 cgcgcaaggg cctacccctg ttttctgatg taatcgtagg ccacgcagac gatcgccgcg
     3841 atggcgatca gcatgatcag aatccccagg gatctagata agtcgcgatc agcatcacga
     3901 acccgatgag gcgagccgca cgctactggt cgagcgcacc caatcgtgga tgaatggctt
     3961 cggcaagctg cgccgctgca cggagcggga t
//
LOCUS       DASYWL010000099         2198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_285866, whole genome shotgun sequence.
ACCESSION   DASYWL010000099 DASYWL010000000
VERSION     DASYWL010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285866"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VGR22_03710"
     CDS             <1..295
                     /locus_tag="VGR22_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127705.1"
                     /translation="MSAIGPEMIDTAAMLVMGRELKVEMETLGRWFAPRRFLERRLVE
                     GSPAPSKTREWLEGEAANQRRVADAFAGRQRRMREAETAVRAWIEETAAESND"
     gene            612..1127
                     /locus_tag="VGR22_03715"
     CDS             612..1127
                     /locus_tag="VGR22_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2127706.1"
                     /translation="MAHPYPRLGRAGTDAEPPVVLVHGLIVASTYMVATAVRLASEYW
                     VYAPDLPGFGDSDKPPQALDVPELAGALVAWMDEVGLERPVLIGNSFGCQIVVDVAAR
                     YPTWVQRAMLQVPTMDRFASTLWQQIGRGLLDLVGEHPSQLIVVAHDFEDRAAPIDQH
                     LPLRSAGPDRG"
     gene            complement(1948..>2198)
                     /locus_tag="VGR22_03720"
     CDS             complement(1948..>2198)
                     /locus_tag="VGR22_03720"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV2127707.1"
                     /translation="FGESRGPNPFVLSEAAGYLAGWMDRLGIEQADIVGHSMGGFIAA
                     ELAADFPNRVARLVLVDAAVLPFGEGYLRHLTGLFRAL"
BASE COUNT          361 a          697 c          777 g          363 t
ORIGIN      
        1 gatgagcgcc atcgggcccg agatgatcga cacagccgcg atgctcgtga tggggcgcga
       61 gctgaaggtc gagatggaga cgctcggccg ctggttcgcg ccgcgccgtt ttcttgagcg
      121 gcggctggtg gagggatcgc ccgcgccctc gaagacgcgc gagtggctgg agggcgaggc
      181 agcgaaccag cgccgggtcg ccgatgcctt cgctggccga cagcgtcgaa tgcgcgaggc
      241 ggagaccgcc gtgcgagcat ggatcgagga gaccgccgcc gaatcgaacg actagcgggc
      301 acgccgggtg ccgcgctgat atcgaccgat gcccctcgat ggggtggaga tggacgttgt
      361 gcgcacccgc aacgcccggc actgccagca tcagggccct ggtgtctttc aatgcggcat
      421 caacgtggct ggcacagatc gcgctgtggc aggcggcatt cggtctgcga gaccggggcg
      481 catcttgcta ctgccgggag gacgaacggc tgggtgccga ggatgacgcg cctctggatg
      541 atgacgccgg cacgcccggc ggaaaccgaa cggatcgggc gactggagca ccggtgggac
      601 ctggttggtg gatggcgcat ccatacccgc gcctcggtcg agccggaacc gatgcggagc
      661 cccctgtcgt cctggtccac ggcctcatcg tcgcgagcac ctacatggtg gccacggcgg
      721 tgcgcctggc atccgaatac tgggtctacg ccccggatct gcccggcttc ggcgacagtg
      781 acaagccacc ccaagcactc gacgtgcccg agttggccgg cgccttggtg gcgtggatgg
      841 acgaggtcgg tctggagcgg ccagtgctta tcggcaactc cttcgggtgc cagattgtcg
      901 tcgatgtcgc cgctcgctat ccgacgtggg tccagcgggc gatgctccag gtgccgacga
      961 tggaccgttt cgcgagtact ctctggcagc agatcggtcg tggtctcctc gacctggtgg
     1021 gggagcatcc ttcgcaactt atcgtggtgg ctcacgactt cgaagatcgg gctgcgccga
     1081 tcgatcagca ccttccgctt cgctcagcag gaccggatcg aggataagct gccgctcgtc
     1141 cgcatgccga cgctggtcgt gcgcgggacc cgcgacctgg tcgtgtccca gcggtgggca
     1201 gaggaggtga ccgcgttgtt gccccaaggt cggctcgtgg tgatccccgg ggcaccgcat
     1261 acgcccaact acagcgctcc ttcagccgtt cctggcgggt gcggagaacg agaagggcct
     1321 cctcccccgt gaaagtgctc catgaactgc ctcccggcgt ctctgctcgc caatctgctc
     1381 ggcgccagga aggatggatc ggcaagcccg cgcggtgggc atatccggcg gctgggagtg
     1441 gtgcccatga tctccgagct gatatggttg tacccggtgt atcggtgcag agaggagtcc
     1501 ctgtggacaa tcaggagttg acaattgcgg tggtagcggt ggtcctcgtg ctgctcgtcg
     1561 ccgcgctggt tctgttcctc ccgtaggtca ctcggcgacc caagcgaggt ctatgcccca
     1621 ctatgggccg gtgcgaggcg tgctcgcctc gagaaactcc agcacgacgc ggttgaagac
     1681 ctccgggcgg tcccacatgg ggttatgacc gcccgacggg acgacgacga gctcggcatg
     1741 ccgcagatgg tgctggacct ggagcccgat ttccagcggg atgacggtac cgcgctctcc
     1801 cccgacaagc agcgtcgacg cgtcgatccg cgagagcttc gatcggatgt cggcggtcag
     1861 gagctgccgg gcggcctgaa ggacggcgag tggtcccgcc cgccaggcat ccgtggccag
     1921 gacggacagg aagctgacca gcgtgtatca cagagcgcgg aacaggccgg tcaggtggcg
     1981 caggtagccc tcgccaaacg ggagcaccgc ggcgtcgacc aacacgagcc gcgcgacccg
     2041 atttgggaag tccgccgcca gctcggcagc gatgaagcct cccattgagt ggccgacgat
     2101 gtcggcctgc tcgatgccaa gccgatccat ccaacctgcg aggtagccgg ccgcctcgga
     2161 gagcacgaag gggttcgggc cacggctctc gccgaagc
//
LOCUS       DASYWL010000100        12068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_248818, whole genome shotgun sequence.
ACCESSION   DASYWL010000100 DASYWL010000000
VERSION     DASYWL010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 12068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12068
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248818"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VGR22_03725"
     CDS             <1..606
                     /locus_tag="VGR22_03725"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEV2127708.1"
                     /translation="MVDEATPKRDPIINIRGEKVGLGPLDASMLAAMTRWINDFQTVR
                     TLAMDPMPMTEQQERQWLESASSGQNGIVFAIYDLADKALVGSTNLFHIDQRHHTCEL
                     GIAILDPTRRGKGLGTEAVRLITDYAIHGLEMHNVQLATYEYNHAGQRAYRKAGFKEY
                     GRRREARLHNGQRWDIIFMDVLANEWESPVMERLMAPDDLR"
     gene            629..1282
                     /locus_tag="VGR22_03730"
     CDS             629..1282
                     /locus_tag="VGR22_03730"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEV2127709.1"
                     /translation="MDRDHTAPTINISGEKVALGPLDRSIISLQTAWINDFATTRTLG
                     HDAQPRSLAVNEDWFEHMAASGATIAFTIYDLSDMAPVGTVNLFRIDHQHGTCTLGIS
                     IMPPERRGRGLGTDAVRLITDYAFNDLGMHNVQLTTLEFNIASIKAYRKVGFQEYGRR
                     REAWLHSGRYWDLIYMDILAPEHQAARLRHPERSEESPREAHRLQSSIPTHDCIDRQ"
     gene            complement(1448..1975)
                     /locus_tag="VGR22_03735"
     CDS             complement(1448..1975)
                     /locus_tag="VGR22_03735"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: protein motif:HMM:NF001664.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEV2127710.1"
                     /translation="MPVSDGYVEVVQGDITRQKVDAIVNAANNQLCGGGGVDGAIHRV
                     AGPELDEACREIGWCPTGEARVTPGFRLPAKWVIHTVGPVWQGGDRDEDALLASCYRS
                     SMELASQHGMLSIAFPSISTGVYGFPVKRAARIAIDTLRDTAQRYPEIETIRVVTFGE
                     NTTRAYEDVVQEYLD"
     gene            complement(2194..3066)
                     /gene="sucD"
                     /locus_tag="VGR22_03740"
     CDS             complement(2194..3066)
                     /gene="sucD"
                     /locus_tag="VGR22_03740"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEV2127711.1"
                     /translation="MSILVNNDTRLLVQGITGREGSFHTTQMVEYGTNVVAGVTPGRK
                     GQEVAGVPVFDTVREAVEATQANTSIIYVPARSAPDAIYEAVDNGIGLVICITEGVAV
                     NDMVPVYHDVKDKGARLIGPNCPGVITPGEAKVGIMPGFIHKPGKVGLVSRSGTLTYE
                     VVNALTEAGMGQSTAAGIGGDPIIGTSFVDALELFQKDQDTEAIVMIGEIGGTDEEDA
                     AEFIKSYVTKPMAGFIAGQTAPPGKRMGHAGAIISGGAGTAVEKMKALEAAGVRVAES
                     PSQVPELLKEAMNA"
     gene            complement(3131..4264)
                     /gene="sucC"
                     /locus_tag="VGR22_03745"
     CDS             complement(3131..4264)
                     /gene="sucC"
                     /locus_tag="VGR22_03745"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF001913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEV2127712.1"
                     /translation="MDVHEYQAAEVLASHGVPVNPGKVATTPDEAEAIAAELGGTVVV
                     KAQVHTGGRGKAGGIKLAKSPGEAREAAEAILGMDIRGHTVNKVLVARGVEIASEFYL
                     GVVLDRPNRKVLVMASAEGGVEIEEVAKTNPEKIVRKLGDPLVGFRPYQGRELGFELG
                     LPADKVNEFAAIAQKLYEVYLTEDATLVEINPLILTDNGDWLAIDSKMSFDDSALPRH
                     PNIEQMRDLAEENETEIEAKNNGISFVKLDGTIGCIVNGAGLAMATMDAVKLHSGEPA
                     NFLDVGGGANAAQVAKAFNLVTADPNVKAILINIFGGITRGDEVANGIREALGQVKVE
                     VPIIVRLSGTNADEGKDILAGAGITAVTSMDHAAGEVVRAASA"
     gene            4627..4965
                     /locus_tag="VGR22_03750"
     CDS             4627..4965
                     /locus_tag="VGR22_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEV2127713.1"
                     /translation="MSDRTLTCSDCGSEFTFTAGEQEFYTQRGYSEPRRCQPCRQARK
                     SQRNQADGGYGDSSGFRSGGGGGQRQFYPAVCSECGQETEVPFSPTPGKPVYCRDCFQ
                     SRRSPRYSAY"
     gene            5081..5734
                     /locus_tag="VGR22_03755"
     CDS             5081..5734
                     /locus_tag="VGR22_03755"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2127714.1"
                     /translation="MPYDRHEQSSNAQSAPLVRVGRLVELRRHTEANRESFIRWYGDR
                     EIAELLRHDLDPLSRREAKAYFTNTILPLSERGQCWAIHRRDTGQLIGTAAVTDVNAV
                     RGTCLYRIVIGEKDAWAHGFGTEATRLVAEIVFETLPITRFRLEVFAHNERARRAYAR
                     VGFQQYDRYEEPVPGKGKTLDIIVMELRPDALAPNVPSLSEDHESRPFPDPDGATRA"
     gene            5909..7849
                     /gene="dxs"
                     /locus_tag="VGR22_03760"
     CDS             5909..7849
                     /gene="dxs"
                     /locus_tag="VGR22_03760"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEV2127715.1"
                     /translation="MPSTSPSATPTLDRISNPADLRALKPKELTALAAEIREELIETV
                     TLTGGHLGASLGTVELAIALHAVFHSPDDKLVWDVGHQAYVHKLLTDRRDRFGTIRQE
                     GGLSGFLSRDESEHDMFGAGHASTSISAGLGMAVAEARKPKGQKRNSVVSIIGDGALT
                     GGMAFEGLNNAGHMKDVPFIVVLNDNEMSIAPNVGALSKYLDRVRTDTRYNRAKDELA
                     ERLEHLPQGDFLVEFGKRMKDSFKEFIYHSMIWEELGFTYIGPVDGHNIPALMEAFRR
                     ARDLREPVFIHVVTQKGKGYDPADNDHERSHAVSAPSKPRAVTAAPTPPKYQDVFADA
                     LIELAEQDERIMAITAAMPGGTSLGKFGERFPKRFFDVGIAEQHAVTFAAGLATQGLR
                     PVCGIYSTFLQRAFDQIIHDVCIQKLPVIFAMDRAGFAGDDGRTHHGVFDLSYLRLIP
                     NMTLMAPKDENELRHMVATAIEHTEGPIAFRYPRGASLGVPYDLEFRPLPVGKGELVR
                     EGSEIALVAVGPMVLAAERAADALAEQGVSATVVNARFIKPLDEELLLGLARTHQGIV
                     TVEENTVLGGFGSAVLELFNREQVETPVRVAAIPDRFFEQALQQRLREMAGLGIDDIV
                     AHATDLMRNAPSALLEQQPTTV"
     gene            7878..8570
                     /gene="rsmG"
                     /locus_tag="VGR22_03765"
     CDS             7878..8570
                     /gene="rsmG"
                     /locus_tag="VGR22_03765"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEV2127716.1"
                     /translation="MRESQLSGLQREQLVRYRDLLLEHNRRINLTAIRDPEGIDRRLI
                     EESLRLLPIIDDLSASGDEVVDIGTGGGVPGMPLAIARPELRWTLIDATAKKVAFLQD
                     VVSTLSLPNVVLYHGRIEELAHESHLRGRYRVLTARAVASLPALLELGLPMVRVGGAL
                     LLPKGLDIDEELAQAAIASRELGGRIVSHDLLPDAGTGVDTRLMIAEKLGETPAAYPR
                     RAGIPARSPLGG"
     gene            8610..9218
                     /locus_tag="VGR22_03770"
     CDS             8610..9218
                     /locus_tag="VGR22_03770"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEV2127717.1"
                     /translation="MTIQNERCQWKCPTRPRIFYRKEKLRGACWEYEIAGGWISVPSP
                     ARESRRILRHEFRFYRPRETVIRTEQGEQVVQRTAAEQWFFPDRWFSLLRFTTADDRT
                     VGYYVNFSHPLRELRENYYRDIDLELDLWLDTDGTMTELDRDEFEQEIAVQRLPAEWA
                     KQVTEAAQSVTRAVSFCLDHHGPDIECTRDPIFGIPEFILRA"
     gene            complement(9276..9836)
                     /locus_tag="VGR22_03775"
     CDS             complement(9276..9836)
                     /locus_tag="VGR22_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEV2127718.1"
                     /translation="MIFPIIGAKDYIDQRGFLVHECPACRAQRAFAVYNTRRKLTLYL
                     IPTVGVRSQHVMECMTCHGRWGISDEQWDEVEPALMTQEELAEWISGRANGRQRQPEP
                     TRLPPTLYQVLQVDPSADPEIIDVAYKRLAMKHHPDRGGGPAAQEQMRRLNHARDVLR
                     DPQRRAEYDRSIGIVRLPDGLRPEDV"
     gene            9995..10699
                     /locus_tag="VGR22_03780"
     CDS             9995..10699
                     /locus_tag="VGR22_03780"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEV2127719.1"
                     /translation="MTQRAHAAPLTPTFVTRWIETLPDVRIEEVAPVPATTGIFSADL
                     VNGFLREGPLASPRIDALTDPVLRLLNRAWEYGVKHYVFLQDTHTMDNPEFRAYPPHC
                     IAGTSESEMIPEIGRLPFANAFHVVEKNSLHPAVETTFNELWDEQRHINRAIVIGDCT
                     DLCVNHLAMHLRMRANALGLQQFDVIIPANCVDTFDIPESEDLDPGGAHPGDFFHEVF
                     LYHLASNGIRVVNSII"
     gene            10749..>12068
                     /locus_tag="VGR22_03785"
     CDS             10749..>12068
                     /locus_tag="VGR22_03785"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF009131.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEV2127720.1"
                     /translation="MSDDRRGFVHEPSHLYTDERGHLFLPESTALFVDLYHIDAAYVA
                     WQNDHTGRATFDLYTRSNPFAGGFMLVAGLEPALDYLQRFEYTDEQLAYLEDVKHYEP
                     AFLQFLSELRFSGDIHGMPEGEITFPNEPLLRVTAPFHEALLLESGLLRAIGVSTLIA
                     TKAARLLIAAGNASVADFAFRRAHAPHLAARSGYIGGCTSTSFVAGAMEYGIPPSGTV
                     PHALVQSYPTEVDAFRGVAASLPVYSLLLDTYDVPHGIRNAIRIAREERERSGHRLAA
                     VRLDSGDLIADSKMVRDHLDEAGMTDVKVLVSGDIDEYRIAEILGAGAPVDGFGVGGN
                     LGVGLGTVASGTVGGVIGAVYKLVWFDSDEQFPSRIKVAGSKSTWPGRKVVYRVGDFE
                     KDTVQLDTEEAPAGSRELLQPIVKNGTRVSTEESLQAIRERAMASLDA"
BASE COUNT         2172 a         4179 c         3654 g         2063 t
ORIGIN      
        1 atggttgacg aggcaacacc gaagagggac ccgatcatca acattcgcgg cgagaaggtt
       61 ggcctcggtc cgttggacgc atccatgctg gccgccatga cgcgctggat caacgatttc
      121 cagaccgtgc gcaccttggc aatggacccg atgccgatga ccgagcagca ggagcgccag
      181 tggctggaga gcgcatcgag cggccagaac gggatcgtct tcgcgatcta cgatctggcc
      241 gacaaggccc tcgtcggctc gaccaacctc tttcatatcg accaacggca ccacacctgc
      301 gagcttggca tcgcgatcct cgatcccacc cggcgcggca aggggttggg aaccgaggcg
      361 gtccggctga tcaccgacta cgccatccac ggcctcgaga tgcacaacgt ccaactcgca
      421 acctatgagt acaaccacgc gggacagcga gcctacagaa aagcggggtt caaggagtat
      481 gggcgccgcc gggaggcacg gcttcacaac gggcaacgct gggacatcat cttcatggat
      541 gtcctcgcca atgagtggga aagccccgtg atggagcggc tcatggcgcc agatgacctc
      601 cgctgatcga aggcaataga cggagagcat ggacagggat cacacggccc ccaccatcaa
      661 catttcgggg gagaaggttg ccctcgggcc tctcgaccgc tccatcattt cgctccagac
      721 agcctggatc aacgatttcg ccaccacgcg cacgctcggc cacgacgccc agccgcgctc
      781 cttggcagtg aatgaggact ggttcgagca catggccgcc agtggcgcga cgatcgcctt
      841 cacgatctac gacctctcgg atatggcacc ggtgggcacc gtcaacctgt tccggatcga
      901 ccatcaacac ggcacctgca cgctggggat atccatcatg ccgcccgaac gacgcggtcg
      961 gggcctcggg acggacgccg tgcggctcat caccgactac gccttcaacg atcttggcat
     1021 gcacaacgtc cagctcacca cactggagtt caacatcgcc agcatcaagg cgtatcgaaa
     1081 ggtcgggttc caggagtatg gccgccgccg agaggcatgg ctccacagcg gcagatactg
     1141 ggaccttatc tacatggaca ttctggctcc tgaacatcaa gcagcccgcc tccgtcaccc
     1201 tgagcgcagc gaagagtccc cgcgcgaagc gcatcgccta cagagcagta tcccaactca
     1261 cgattgcatt gatcgtcagt gagtacggcg cctgcggtgc cggtgcttcg cacgagaacg
     1321 ccgcgataga tcggcagcgg acctccggcc cttgctcagg acaggctggc tgcggccgac
     1381 gttcagcacg acgccggaga ggcagctggc tatgatcctg ggcgggcctc caagcgcggg
     1441 atcgtcgtca gtccagatat tcctggacca cgtcctcgta cgcccgggtc gtgttctccc
     1501 caaaggtgac cacccggatc gtctcgatct ccggataccg ctgggccgta tcgcgcagcg
     1561 tgtcgattgc gatccgcgcc gcccgtttca caggaaatcc atacaccccg gtactgatcg
     1621 acggaaaggc aatcgacagc atgccgtgct ggctcgcaag ctccatcgat gaccggtagc
     1681 agctcgccag cagcgcgtcc tcgtcgcgat caccaccctg ccagacgggc ccgacagtat
     1741 ggatcaccca cttcgctggc agccggaacc ccggcgtgac ccgcgcctcg ccggtcgggc
     1801 accagccgat ctcgcggcac gcctcgtcga gctcaggccc cgccacgcga tggatggcac
     1861 catcgacgcc accgccgccg cacagctggt tgttggcggc attgacgata gcatcgacct
     1921 tctgccgcgt gatgtcgccc tgcaccacct cgacatagcc gtcactgacc ggcatcgcca
     1981 atgctcccgg agtaaacaca cagagaacct aacatctacc tgagcttgcc aagagccccc
     2041 gcacgcagcg ccgacagcat tgctgcaact tctcactgcc cggcgctgcc agaaatcggc
     2101 tcctggctct catcacgctg cacttcgcgc agggattcct cgagttcggg cctgcggccc
     2161 tcgcccggaa tggcgatgtt gacttcgtcc tgactacgcg ttcatcgcct ccttgagcaa
     2221 ctcgggcacc tgcgacgggc tctctgcgac ccgcacgcca gccgcctcca gcgctttcat
     2281 cttctcgacc gccgttccgg caccgcccga gatgatcgcg cctgcatgcc ccatccgttt
     2341 gcccggaggc gccgtctgtc cggcgatgaa accggccatc ggcttggtca catacgactt
     2401 gatgaactcg gcggcatcct cctcgtcggt gccgccgatc tcgccgatca tcacgatcgc
     2461 ctcggtgtcc tggtctttct ggaacagctc cagtgcgtcg acgaacgagg tgccaatgat
     2521 cggatcaccg ccgatgccgg ccgccgtcga ctggcccatg cccgcttcgg tgagcgcgtt
     2581 cacgacctca taggtcagcg tgccagagcg cgagacgagt ccgaccttac cgggcttgtg
     2641 gataaaaccc ggcatgatgc cgaccttggc ttcgccgggc gtgatcacgc cggggcagtt
     2701 cgggccgatc agtcgcgcgc ccttgtcctt cacatcatgg tagaccggta ccatgtcgtt
     2761 gaccgccaca ccttcggtga tgcagatcac cagaccgatg ccgttgtcga ctgcctcata
     2821 gatcgcgtcc ggcgcggagc gggcaggcac gtaaatgatg gaggtgttgg cctgtgtcgc
     2881 ctcgaccgcc tcccggacgg tgtcgaagac cggaacgccg gcaacctcct gccccttgcg
     2941 gccgggcgtc acgccggcta caacgttggt gccgtactcc accatctgcg tcgtgtggaa
     3001 ggagccctca cgaccggtga tgccctgcac cagcagacga gtgtcgttat tgaccagaat
     3061 gctcacgcgt tgctcctcat cgtcatcccg gcctccccgc caggatccgt cccttcggta
     3121 ttggaatagc tcacgcgctg gcggccctga cgacctcgcc ggccgcatgg tccatcgagg
     3181 tgacggcggt gatgcccgcg ccagcaagga tgtccttgcc ctcatcggca ttcgtgccgg
     3241 aaagccgcac gatgatcggc acctcgacct tcacctgtcc cagcgcctcc cggatgccgt
     3301 tggcaacctc gtcaccccgc gtgatgccgc cgaagatgtt gatcaggatc gctttcacat
     3361 tcgggtccgc ggtcaccagg ttgaacgcct tggcgacctg agcggcgttg gcgcctcctc
     3421 cgacatcgag gaagttggcc ggctcgccgc tgtgaagctt gacggcgtcc atggttgcca
     3481 tcgcgagacc cgctccgttc acaatgcaac cgatggtgcc atcgagcttc acgaacgaga
     3541 taccgttgtt cttggcctcg atctccgtct cgttctcctc ggccaggtcg cgcatctgtt
     3601 cgatgttcgg gtgacgcggc agcgcgctgt cgtcgaagct catcttggag tcgatcgcca
     3661 gccagtcgcc attgtcagtg agaatcagcg gattgatctc cacgagggtt gcatcttcag
     3721 tgagatacac ctcgtagagc ttctgggcga tcgctgcgaa ctcgttgacc ttgtcagccg
     3781 gcagccccaa ctcgaagccg agctcgcggc cctgataggg acggaatcca accagcggat
     3841 cgcccagctt gcgcacgatc ttctcggggt tggtcttcgc aacctcctcg atctcgacac
     3901 caccctcggc actggccatc accagcacct tgcggttggg tcgatcgagc accacaccca
     3961 ggtagaactc gctggcgatc tctacccccc ttgccaccag caccttgttg accgtgtgac
     4021 cgcgaatgtc catgccaagg atcgcctcgg cagcctcgcg cgcctcccct ggtgatttcg
     4081 cgagcttgat gccgccggcc ttgccgcgac cgccggtgtg cacctgcgcc ttcacgacca
     4141 ccgtgccgcc aagctccgcc gcaatggctt cggcttcgtc cggcgtggtc gcgaccttcc
     4201 cggggttgac cggaacgccg tggctcgcca gcacttccgc cgcctgatac tcatggacat
     4261 ccatcgagtg ccccactcct tgcttgcaac tacccgccgg gcaacccatc ggccaaactg
     4321 acaccgagtg atcaaggctt ttccccaacg tccccggcat ggtcctgact gctgcatcct
     4381 agcacagggc gtgccatctc ccgaaacgcc gaaaatcctc gaactcttcc atctttcggc
     4441 aaggaaacca tggtatgatg aggcagccga gacgtttcca gcaggtcaca cccgccaggg
     4501 caatcgccgg tgccctctcg cggctgtttc aactcggaac cggatcggat cgaagttccg
     4561 ttcggctttc gtgccacagt aaggaatcgc gggcgcggcc cgtgaatcgg agaggttcag
     4621 gcgagaatga gtgatcgaac gctgacatgc agtgactgcg gcagtgaatt cacgttcacg
     4681 gctggcgagc aggagttcta cacgcagcgc ggatattccg agccccgtcg atgccagcca
     4741 tgccgacagg cccgcaagag ccagcgcaac caggccgatg gcgggtacgg ggacagcagc
     4801 gggttccgca gcggtggtgg cggcggccag cgccagttct acccggcggt ctgctctgag
     4861 tgcggccagg agaccgaggt tccgttctcc ccgacgccgg gcaagccggt atactgccgt
     4921 gactgcttcc agtcgcgtcg cagcccgcgg tactccgcct actagcgacg actccacctg
     4981 cggttctgca ggtgtgatgg gggcaggcca acgcctgccc ccgtttctcg ttccggccct
     5041 cagtcgcgtc ccatccgccc acaaagacgg acgccgcctc atgccgtatg atcgccacga
     5101 acaaagcagc aacgcgcaat cggcaccgct cgtccgcgtc ggccgactgg tcgagctgcg
     5161 acggcacacc gaagccaatc gcgagtcctt catccgctgg tacggcgacc gcgagatcgc
     5221 cgagctgctg cggcacgacc tggatcccct gagtcgccgc gaggccaagg cctacttcac
     5281 caacacaatc ctcccgctct cggagcgagg acagtgctgg gccattcacc ggcgcgacac
     5341 cggccagctc atcggcaccg ccgccgtgac ggacgtaaat gccgtgcggg gcacgtgcct
     5401 ctatcgcatc gtgatcggag agaaggatgc ctgggcgcac gggtttggca ccgaggcgac
     5461 ccggctcgtc gccgagatcg tcttcgagac gctgcccatc acgcgtttcc ggcttgaggt
     5521 cttcgcgcac aacgaacgcg ctcgccgagc ctacgcccgt gtcggcttcc agcagtacga
     5581 tcggtacgag gagcccgttc ccggcaaggg caagacgctc gacatcattg tgatggagct
     5641 tcggccggac gcgctggcac cgaacgtgcc ttcactgtcg gaagatcacg aatcccgccc
     5701 cttccccgac cccgacggcg ctacgcgagc gtagtcatcg cccggatgcc ggtggaagcg
     5761 ccgggagtgc attggtatcg ttcgcatagc cataaaccgc caggtgccgc cggcgcggta
     5821 aggtatgggt tgcctgcttc cagcctgagc cgagcctttg tccgcgcgct gagggctgac
     5881 gctggaccca cgaggaagaa agaccaccat gccatccaca tcaccatcgg ccaccccgac
     5941 gctcgaccgg atctccaatc cagccgacct cagggcgctc aagccaaagg agttgacggc
     6001 gttagcggcc gagatccggg aggagctgat cgaaacggtc acgctgacgg gcggtcatct
     6061 cggggccagc ctcggcacgg tggagttggc gatcgccctg cacgccgtct tccactcgcc
     6121 tgacgacaag ctcgtctggg acgttgggca ccaggcctac gtccacaagc tgctcaccga
     6181 ccggcgcgac cgcttcggca ccatccgaca ggagggcggt ctcagcgggt tcctgagtcg
     6241 cgacgagtcc gagcacgaca tgttcggtgc cgggcacgca tcgacctcga tctccgccgg
     6301 actcggcatg gccgtcgcgg aggcgcgcaa gccgaagggc caaaagcgca acagcgtcgt
     6361 ctcgatcatc ggcgatggcg cgctcactgg cggcatggcg ttcgagggcc tcaacaacgc
     6421 cggccacatg aaggatgtcc ccttcatcgt ggtcctcaac gacaacgaga tgtcgatcgc
     6481 gccaaacgtc ggcgcgctca gcaagtacct cgaccgagtc cgcaccgata ctcgctacaa
     6541 ccgtgccaag gatgagctgg ccgagcgcct ggagcacctg ccgcaaggcg acttcctcgt
     6601 cgagttcggc aagcgcatga aggactcctt caaggagttc atctaccact cgatgatctg
     6661 ggaagaactc ggcttcacct acatcggtcc ggttgacggc cacaacatcc ccgcgctcat
     6721 ggaggcgttc cgtcgcgctc gcgacctcag ggagccggtc ttcattcacg tggtgacgca
     6781 gaagggcaag ggctacgatc ctgccgacaa cgaccacgaa cgcagccatg cggtcagcgc
     6841 gcccagcaag ccgagggcag tgacggcggc accgaccccg cccaagtacc aggatgtctt
     6901 cgccgacgcg ctgatcgagc tggccgaaca ggacgagcgg atcatggcaa tcaccgccgc
     6961 gatgcctggc ggcacatcgt tgggcaagtt cggcgagcgc ttcccgaagc ggttcttcga
     7021 tgtcggcatt gccgaacagc acgccgtcac cttcgcggcg ggcctcgcga cgcagggcct
     7081 tcgcccggtc tgcggcatct actcgacctt cctgcagcgc gcgttcgacc agatcatcca
     7141 cgacgtctgc atccagaagc tgcccgtgat ctttgcgatg gaccgtgccg ggttcgccgg
     7201 cgacgacggt cgcacgcacc acggcgtgtt cgatctcagc tatctgcggc tcattcccaa
     7261 catgaccctc atggcgccga aggatgagaa cgaacttcgg cacatggtgg cgacggcgat
     7321 cgagcacacc gaaggcccga tcgcctttcg ctacccacgc ggtgcgagcc tcggtgtgcc
     7381 gtacgatctg gagttccggc cgctgccggt cggcaagggc gagctggtcc gcgagggatc
     7441 ggagatcgcg ctggtcgcgg tcggaccgat ggtgctcgcc gccgagcgtg cggccgatgc
     7501 gctcgccgag cagggcgtct cggctacggt cgtcaacgct cgcttcatca agccacttga
     7561 tgaggagctg ctgctcgggc tagcacggac ccatcagggc atcgtgaccg tcgaggagaa
     7621 caccgtcttg ggcggtttcg gctccgcggt gctcgagctg ttcaaccgtg agcaggtcga
     7681 gacgcccgta cgcgttgccg cgattccgga ccgcttcttc gagcaggcct tgcagcagcg
     7741 cctgcgggag atggccggac ttgggatcga tgacatcgtc gcccacgcga ctgatctcat
     7801 gcgcaacgcc ccttcggccc tgctggagca acaaccgacg acggtctagc tgccttgcat
     7861 cgaccgggga aaccgccatg cgcgaatctc agctcagcgg tctgcagcga gagcagctgg
     7921 tgcgctatcg tgatctgctg ctcgagcaca accgacgcat caacctgacg gcaattcgcg
     7981 atccggaagg catcgaccgg cgcctcattg aggagagcct gcggctgctg cccatcatcg
     8041 atgacctctc ggcatcgggt gacgaggtgg tcgacatcgg cacgggcgga ggggtgccgg
     8101 gcatgccgct ggcgatagcc cggccggaac tgcgctggac cctcatcgac gcaaccgcca
     8161 agaaggtcgc attcctgcag gatgtcgtaa gcacactctc gctcccgaac gtcgtgctgt
     8221 accatggacg catcgaggag ctggcgcatg agtcgcatct gcggggacgc taccgcgtcc
     8281 tgaccgcccg ggcggtcgcc tcgttgccag cattgcttga acttgggctt ccgatggttc
     8341 gcgtgggagg cgcgctgctc ctgccgaagg gactggacat cgacgaggag ctggcacagg
     8401 ccgctatcgc gtcccgcgag ctcggcggac ggattgtctc ccacgatctg ctcccggacg
     8461 ctggtaccgg cgtcgacacc cggctcatga ttgccgagaa gctgggggag acaccggcag
     8521 cctaccctcg acgcgccgga attccagctc ggtccccgct tggaggatag acgcgcgcca
     8581 ccggaagcca gtaacggtat cggaccgtca tgaccatcca gaacgaacgc tgccaatgga
     8641 agtgtccgac gcgcccgagg attttctacc gcaaggagaa actccgtggg gcctgctggg
     8701 agtacgagat tgccggtggc tggatcagcg tgccgtcacc ggcccgcgag tcgcgccgta
     8761 tcctgcgcca tgagtttcgg ttttaccgtc cgcgtgagac ggtgatccgc accgagcagg
     8821 gcgagcaggt agtccagcgc acggccgccg agcagtggtt cttcccggac cgctggttct
     8881 cgcttctgcg attcaccacg gccgacgacc gcaccgtggg ctattacgtc aacttttcgc
     8941 accctctccg ggaactgcgc gagaactact accgcgacat cgacctcgag ctcgatctct
     9001 ggctcgatac cgacggcacc atgaccgagc tcgaccgtga tgaattcgaa caggagattg
     9061 ccgtacagcg gctgccagcc gagtgggcga agcaggtcac cgaggcggcg caatcggtca
     9121 ctcgagccgt gagcttctgt ctcgaccatc acggcccgga catcgaatgc acccgcgacc
     9181 ccatcttcgg catccccgag tttatcctcc gggcatgagc gtcaacgcca cgttgagcga
     9241 gagtccggct gtagccgacc ggcggggacc atggttcaca cgtcctcggg gcggaggccg
     9301 tcggggaggc ggacgatgcc gatcgaccgg tcgtactcgg cacggcgctg cggatcccgc
     9361 aggacatcgc gcgcatggtt gagcctgcgc atctgctcct gcgcggcggg accaccgccc
     9421 cggtcggggt ggtgcttcat cgccagccgc ttgtaggcaa cgtcgatgat ctccgggtcg
     9481 gctgacgggt cgacctgcaa cacctgatag agcgtcggcg ggagacgcgt cggctccggc
     9541 tgccgctgcc ggccgttcgc ccggccggag atccattccg ccagctcctc ctgtgtcatc
     9601 aacgccggct cgacctcgtc ccactgctcg tcagagatcc cccagcggcc atgacaggtc
     9661 atgcactcca tcacgtgctg cgatcgcacg ccaaccgtcg ggatcaggta gagcgtcagc
     9721 ttgcggcgcg tgttgtagac ggcgaacgcc cgctgcgccc gacacgccgg acactcgtgg
     9781 accagaaacc cacgctgatc gatgtaatcc ttcgcgccaa tgatcgggaa tatcacccgg
     9841 catgtctcct gcgtcccgcg ttcagccagc ttgagcctac caagtcgccg acagtgcgac
     9901 cagcaacccc cggcacgcgc gatacgctgc gtgcgaaaat gggcggaact gccgcgccgg
     9961 ccacaccggc gccccgttcc ggaggtaccc cgccatgacg caacgtgccc acgcggcccc
    10021 gctgacaccc accttcgtga cccgctggat cgagaccctg cccgacgtcc gcatcgagga
    10081 ggtcgcacct gtccctgcca ccaccggcat cttctccgcc gatctggtca atggcttcct
    10141 ccgcgagggg cccctcgcct ccccacgcat tgacgcgctc acggatccgg tcctgcggct
    10201 gctcaaccgc gcgtgggagt acggcgtgaa gcactatgtc ttcctgcagg acacccacac
    10261 catggacaac ccggagttcc gagcctaccc gccgcactgc atcgcgggaa ccagcgagag
    10321 cgagatgatc ccggaaatcg gccgcttgcc gttcgccaac gcctttcacg tcgtcgagaa
    10381 aaactcgctc caccccgccg tggagaccac cttcaacgag ctctgggacg agcagcgcca
    10441 catcaaccgg gcgatcgtga tcggcgactg cacggacctc tgcgtcaacc acctcgcgat
    10501 gcacctgcgc atgcgcgcca acgcgctcgg cctccagcag ttcgacgtga tcatcccggc
    10561 gaactgcgtc gacacgttcg acatccccga gagcgaggac ctcgatccgg gtggcgcgca
    10621 cccaggcgac tttttccacg aggtctttct ctaccacctc gcctccaacg gtatccgggt
    10681 ggtgaacagc atcatctgac cactcgcgcg gtcaccgtcg ccacgaatgt catggagagg
    10741 caccacggat gagcgacgac cggagaggat tcgtgcacga gccatcccac ctctataccg
    10801 acgaacgtgg gcacctgttc ctgcccgagt cgacggctct cttcgtcgac ctctaccaca
    10861 tcgacgccgc ctacgtggcg tggcagaacg accataccgg ccgggcgacc ttcgacctct
    10921 acacccgttc caacccgttc gccggcggct tcatgctggt ggccgggctg gagcccgcgc
    10981 tcgactacct gcagcggttc gagtacaccg acgagcagct cgcctacctc gaggacgtga
    11041 agcactacga gcccgccttt ctgcaattcc tgagtgagct gcgcttctcc ggcgacatcc
    11101 acggcatgcc cgagggcgag atcacctttc cgaacgagcc gctccttcgc gtgaccgcgc
    11161 cgttccacga ggccctgctc cttgaatccg ggctgctgcg cgctatcggc gtttcgaccc
    11221 tgatcgccac caaggccgca cgcctgttaa tcgccgcagg gaacgccagc gtcgccgact
    11281 tcgccttccg gcgcgcccac gcaccgcacc ttgccgcccg gtccggctac atcggcggct
    11341 gcaccagcac atccttcgtg gccggcgcga tggagtacgg tatcccgccg tccggcaccg
    11401 tgccgcacgc gctggtccag tcctacccga ccgaggtgga cgcgttccgc ggcgtggcag
    11461 cgtcgctgcc ggtctacagc ctgctgctcg acacctacga cgtgccgcac ggcatccgca
    11521 acgccatccg catcgccagg gaggagcgcg aacgttcggg ccaccgtctg gcagcggttc
    11581 ggctcgacag cggggacctc atcgcggaca gcaagatggt gcgcgaccac ctggacgaag
    11641 ccggcatgac cgacgtgaag gtgctggtga gcggcgacat cgacgagtac cgaattgccg
    11701 agatccttgg cgccggggca ccggtcgatg gcttcggcgt cggcgggaac ctcggcgtcg
    11761 ggctcggcac ggtcgccagc ggcaccgtcg gcggagtgat cggcgccgtc tacaagctcg
    11821 tctggttcga cagcgatgag cagttcccct cccgcatcaa ggtcgcgggc agcaagagca
    11881 cgtggccggg ccgcaaggtg gtctaccgcg tgggcgattt cgagaaagat accgtccagc
    11941 tcgacaccga agaggcaccg gcgggaagcc gcgagctgct gcagccgatc gtcaagaacg
    12001 gtacgcgggt ttcgacggag gaatcgctgc aggcgatccg cgagcgagca atggcgtcgc
    12061 tggatgcc
//
LOCUS       DASYWL010000101         1736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_567685, whole genome shotgun sequence.
ACCESSION   DASYWL010000101 DASYWL010000000
VERSION     DASYWL010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567685"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VGR22_03790"
     CDS             complement(<1..192)
                     /locus_tag="VGR22_03790"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEV2127721.1"
                     /translation="MGIMDRISRLVRANVNDLIDSAEDPEQMIDQILRDMQSNITTAR
                     AQVAAMIAQEKELEAEEEET"
     gene            complement(302..1345)
                     /locus_tag="VGR22_03795"
     CDS             complement(302..1345)
                     /locus_tag="VGR22_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127722.1"
                     /translation="MRNSFDERSATAGGTDRTADTVHHHLDHVLGRIPGYRGYRDKED
                     RRGADRAIRDQLAGGLDEAAARVERVARDLADRRRLRDVSTVDAWVQALRHLANRIRN
                     ASYGYGGLFGDRTVDAQALDQLRQFDEAMLADLQRLEDPIGGLEAAVVANGDLSAAVQ
                     RGREVTGRLATQFDSRESVISSGVAEPQRRMQDLLSPAAQDAPAAAWHLDAGDALAIY
                     GSDFLVDARLHVDAGNLTFRLFRLGERGDKRWLFVPKDRDRAPALLTETEAVSTEGLA
                     TDFQGSGQSELVGPGGASEPRVVNVTLLSSPSPQEASRSVILAWPDEQITWQGEGLSH
                     DEISIFGAPTPQS"
     gene            complement(1397..>1736)
                     /locus_tag="VGR22_03800"
     CDS             complement(1397..>1736)
                     /locus_tag="VGR22_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127723.1"
                     /translation="YFLVRGRAEANELQEAAQQRRLLDIIKTRGTVSISDLALDLRTT
                     GDGVQSDLYELVGRGLFTGYVDWSKGVLYSVEAKQLQGRDSCPNCGGQLELAGHGLIK
                     CPYCGAEIFL"
BASE COUNT          299 a          570 c          530 g          337 t
ORIGIN      
        1 cgtctcttct tcctcggctt ccagctcctt ctcttgggcg atcatcgccg ccacttgggc
       61 ccgagcggtg gtgatgttgc tctgcatgtc ccggagaatt tggtcgatca tctgctctgg
      121 gtcttccgcc gagtcgatca ggtcgttcac gttcgcgcgg acgagccgtg agatgcggtc
      181 catgatgccc atcgttcaac ctccctatgc ctaccagacg agccggccac ctccgatggc
      241 ggccaccctc acgtcggtgt ctgttgtacc acgactcccc tgctccagca ggagaagtat
      301 gtcagctctg aggggtcggt gcgccgaata tcgagatctc gtcatggctc aaaccttccc
      361 cttgccacgt gatctgctcg tccggccatg cgaggatgac ggaccgggac gcctcctgag
      421 gggacggaga cgataagagc gtgacgttga cgaccctcgg ttcactggcg ccgccgggtc
      481 cgacgagttc actttgtccg ctgccttgga aatctgtcgc gagtccttcc gtcgacacgg
      541 cttccgtttc agtcagcaaa gcgggtgcgc ggtcgcgatc cttcggcacg aacagccagc
      601 gcttgtcgcc gcgctcgcca agtcgaaaga ggcggaaggt gaggttaccg gcatcgacgt
      661 gcagacgggc gtcgaccagg aaatcgctgc cgtagatggc aagagcgtcg ccagcgtcga
      721 ggtgccatgc agccgctggg gcgtcttgcg ccgcaggcga gagcaggtcc tgcatgcgcc
      781 tctggggttc tgcaacgcct gacgagataa ccgactctcg ggagtcaaac tgagttgcga
      841 ggcgacccgt cacctctctc ccccgctgta cggcagcgga gagatctccg tttgcgacca
      901 ccgccgcttc aaggcccccg atcggatcct ccagccgctg gaggtcagcg agcatggcct
      961 cgtcaaactg acgcaactgg tcaagggcct gtgcatccac cgtgcggtcg ccgaacaggc
     1021 cgccatatcc gtaggaagcg ttgcggatgc ggttggccag gtggcgcagc gcttgcaccc
     1081 acgcgtcgac cgtactcaca tcgcgcaatc ggcgccggtc tgccaggtcc cgggcaactc
     1141 gctcgacccg cgcggcggct tcgtcgaggc cacccgccag ctgatcgcga atggcgcgat
     1201 cggcaccgcg acggtcttct ttgtcgcgat atcccctgta tcccggtatg cgcccgagca
     1261 cgtgatcgag atgatgatgg accgtgtccg cggtacgatc ggtgcctccg gccgtcgccg
     1321 atcgttcatc gaagctattc cgcatcgtca ctctcctcgg accgaatggt ttcccagctc
     1381 atgtgcatct gctggctcag agaaagatct cagcgccgca gtacgggcat ttgatcaggc
     1441 cgtgcccagc gagctccagt tggcccccac aatttggaca cgaatcccgg ccttggagtt
     1501 gcttcgcttc gacagagtac aggaccccct tcgaccaatc gacataaccg gtgaagaggc
     1561 cgcggcccac caactcatag agatcggact gcacgccgtc acccgtcgta cgcaagtcca
     1621 gggcgaggtc ggagatcgac acggtgccgc gggtcttgat gatgtcgagc aggcggcgct
     1681 gctgcgccgc ctcttgcaat tcattggcct cggcccggcc gcggacgaga aagtag
//
LOCUS       DASYWL010000102        19968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_248937, whole genome shotgun sequence.
ACCESSION   DASYWL010000102 DASYWL010000000
VERSION     DASYWL010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 19968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19968
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248937"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..1420)
                     /gene="eno"
                     /locus_tag="VGR22_03805"
     CDS             complement(140..1420)
                     /gene="eno"
                     /locus_tag="VGR22_03805"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEV2127724.1"
                     /translation="MDRVIEAVHAREILDSRGNPTVEVEVFLASGAVGNAAVPSGAST
                     GAYEAVELRDGDKGRYDGKGVLTAVENVNEELAEVVVGMDPLDQVGIDLLMKEVDGTS
                     NKGRLGANALLGISMAVARAAAEELGLPLYRYLGGPNARTLPVPMMNILNGGKHAEGS
                     SVDMQEFMVMPVGAKTFSEALRMGTEVFHTLKKVLSERDLSTHVGDEGGYAPSLESNE
                     AAVQVVLEAIERAGYTAGEDLVIALDPAATEFYEDGQYNLAGEGRTLSSDEMVDFWAE
                     WVDKYPIVSIEDGLAEDDWDAWANLTERVGNWCQLVGDDLFVTNTERLGTGIERGAGN
                     AILVKLNQIGTLTETLEAIEMAHRAGFRAVISHRSGETADTFIADLAVATNAGEIKTG
                     APSRVDRVAKYNQLLRIEEELAETAAYPGRSILP"
     gene            1849..2913
                     /gene="pheS"
                     /locus_tag="VGR22_03810"
     CDS             1849..2913
                     /gene="pheS"
                     /locus_tag="VGR22_03810"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEV2127725.1"
                     /translation="MATTTHGLSEKIDRVRAEATQQLEQIASPEAVQEWERTWIGPKG
                     EITALLRSLGSLPKEERPDAGRETQALRKELEAAFAPVKERMEQQALEERLASEAVDV
                     TLPGIEPRIGAIHPISTMIDELIEIFAQMGFQAVFGPEVETTRYNFDLLNIPADHPAR
                     DVWDTVIVDDPSGEIVLRTHTSPMQARTMERIDPPVRVIVPGAAYRYEAQDASHEWQL
                     TQLEGLAVDEHITMTDLKGVLQEMARQLFGPQRKVRLRCDFFPFVEPGVDFAVDCAIC
                     NGKGCRVCKHTGWLEMGGAGMVHPNVLRGVGYDPEKYTGFAFGLGIERLVMMKYGVDD
                     IRAFASNDLRFLKKFAAAAV"
     gene            3058..5502
                     /gene="pheT"
                     /locus_tag="VGR22_03815"
     CDS             3058..5502
                     /gene="pheT"
                     /locus_tag="VGR22_03815"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEV2127726.1"
                     /translation="MKVPFRWLQEYVDIDISPEEFAQRMTMAGLEAEQIDYVGDGWDK
                     VYVAEVLNLSPHPDADRLVLADVAAGEHRLTVVTGAPNIEKSQKVVLALAGARLIDAY
                     ADELKYKTLKPGKIRGITSEGMVCSEKELGLSDEHEGILVLDDDAPVGAPLADYLGDA
                     IIEFEITPNLVHAFSMVGIAREAAALLEKSYRAPKEADLSTSPSRDDLVTIEAPELAS
                     RYVGVVIEGISIEPSPSWMAQRLNRAGVRPVNNIVDVTNYVLLEMGQPLHAFDLKTIE
                     TGHIVVRRGKANETIETLDHRTREVDEDTLLITDGNRPVGIAGVMGGVNSEVTDDTTA
                     IVLESATFGMVNIRHTSRNLKLRTDASARFERGLDPELAIAAAQRAVHLILELCPNAR
                     VVEHDDTYPNPVEPRQVALPFSRFERVLGIEVDRDQALGVLERLELQPRLDDATGVLT
                     VTVPTWRSDITIPEDVVEEAARMIGYHLLPATLPTGTTPEVERDGMFLLERKVRNMLA
                     GGGMYEGRSYVTLSEEEVDRWSSGTSLSLTAEVPTDAAVQLLNPVNTERPLLRPSLLP
                     SLLPAVGDNLKHQQSVRLFEVAHVYASNGPGQLPNETSNVAGVLAGRREELDRFNPAS
                     DEMDFWDAKGVVDALGARLGLTLSYERTTHPAFHPGRTASVLAEGTRLGIVGEIHPNL
                     TDEYGVEARTAAFELSLDALQGAMPDIRGIQVTTDQYLPVQQDFSIVVDADTAAADVE
                     ASLMQGAGPLATDLVLFDVYQGDNIEAGKKSLTFRVTFTAPNRALTDKELEKVRKKIE
                     KTLQRGTAATLRAVES"
     gene            5505..6863
                     /gene="hisD"
                     /locus_tag="VGR22_03820"
     CDS             5505..6863
                     /gene="hisD"
                     /locus_tag="VGR22_03820"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEV2127727.1"
                     /translation="MATSTITIRRIENFDEALAELTRRGSFLDPQLSDRIREGIRQVF
                     GEDLSAREVVERIVTDVRSQGDAALRRYTEAFDGQTRDSFEVPKDDWRSARERLDADV
                     VAAMETAVGRIQAFHERQPGRSWLHPTPQGTFGQIVRPLERVGLYTPGGTAAYPSSLL
                     MSAVPARVAGVQEVVIATPPARDGRIAPTILAAADIAAADRVFAMGGAQAIAALAFGT
                     ETVPHVDKIFGPGNIFVTLAKQRVYGVVDLDQLAGPTETLLIADETADVELVAADLIA
                     QSEHDTIASALLITTSVGLADAVPNEIERQIAELERAYIIRASMSTNGRIIVTGTLDE
                     AVRLANAYAPEHLCLLTARPWDLVPNVENAGGIFVGEDSPEALGDYTAGPSHVMPTGG
                     TARFSSPVHVGEFQKVISLLAASPQAVDELGEATIELANAEGLTGHASAIRRRLEKRK
                     KP"
     gene            complement(6985..7575)
                     /gene="ruvA"
                     /locus_tag="VGR22_03825"
     CDS             complement(6985..7575)
                     /gene="ruvA"
                     /locus_tag="VGR22_03825"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEV2127728.1"
                     /translation="MIAGLRGTVARLEPGAVILDVNGVLYRVHVPASMTQGGGLETGQ
                     RLDLETHMVVREDSMTLYGFESVSDVEWFRVLIGLNGVGPKVALALMSKFSADEMAGI
                     VHREDVMLLSTVPGLGKKTASRILLDLRGKIPEPTGTPLTGQVRMQDDDLIEALEGLG
                     YSRVEAVGAASRVDLSPDATLEDRLLSALRQLTPSG"
     gene            complement(7572..8435)
                     /locus_tag="VGR22_03830"
     CDS             complement(7572..8435)
                     /locus_tag="VGR22_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV2127729.1"
                     /translation="MPSPVVDAHVHLFPPDVIAGKDHYCERDAWFGVTHAAWPVERFP
                     QAEHLLESMDEAGVSMAVIAAWPWVDPGHCRAHNDWLAEVAATSPRLAWLAIVSPADA
                     SAPQELERVRGLGAVGIGELNADAQGFAWEREGDVRELAEIATTLDFPIMAHVSEPVG
                     HDYPGKGTATPPKILAFLEQHPDLRFVAAHWGGGLPFYELMPEVGLALRNVTYDTAAS
                     TYLYRPEVFDVVNRIVGPRRVVWGSDYPVLGQRRFLAKARSILDDETADAVVGANAAR
                     VYGLRVREVAT"
     gene            complement(8453..9349)
                     /locus_tag="VGR22_03835"
     CDS             complement(8453..9349)
                     /locus_tag="VGR22_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEV2127730.1"
                     /translation="MVLTEQRAVEVEVGSASRAGGRDHNEDAVLAVQIGDGGNAWLLA
                     VADGMGGHEGGEVASRLGIETLQQQFAGPLPDDVALELKRAYRQANDRIWAEGGQQSA
                     THMGTTLVAAVINGSYLTIANVGDSRAYLIRGSQVLQVTQDHSLVAEQVERGEIEAGT
                     ERQSPQRNVLTAAVGTTERLDRKLPAIYELSLIPEDRLLLCSDGFHDVVEQRDYAAQL
                     AGGDPSTLAMSLASLAEQRGTTDNVSAVVLIAKPSRATEQRQQLETTIAEQRPQGNAI
                     VVTVVLVLVVIAIAALALVFLL"
     gene            9483..10217
                     /locus_tag="VGR22_03840"
     CDS             9483..10217
                     /locus_tag="VGR22_03840"
                     /inference="COORDINATES: protein motif:HMM:NF018822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LppX_LprAFG lipoprotein"
                     /protein_id="HEV2127731.1"
                     /translation="MRLLGILTITLLLLATSSLAPVLAQPGGTATPASESEAQALLRK
                     ASERLAETETVRFNLEVEGDTYIDEEQDIRLINARGNLARPDSVDVEFQVELLGTQTV
                     SIRMISIGDESWTTDLLTGEWAPSPEEFGYNPTVLFDNQKGLGPVAGRLVDPEIVDKV
                     EIGGREAWKVVGTVDEEVIEPITSGTMEGEEITVTLWLDTETYDMLRIRVEEPKDIAK
                     ENPATWVLSLTGHDSDLTIQRPDIDE"
     gene            10214..11812
                     /locus_tag="VGR22_03845"
     CDS             10214..11812
                     /locus_tag="VGR22_03845"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2127732.1"
                     /translation="MTQRSAQASPWRDPLSLIMIFAVFVGAIDLTVVATILPRMITDL
                     GINTADIDRYIWVVNSYLIGYVVSIPLFGRLSDILGLRNALVSSSLFFIVGSWWCAQA
                     ESLRDLVIGRAVQGFGAGALLPLALAAAAASFTGAKRANAVGAIGASDEIGWVSGPAY
                     GALVVNQLQIIDEPWRLIFWVNIPIALALVGLAWRKLGGLQPPRYHHPGSFLQRLDLP
                     GFVLISTFLVAINLALASGGEIGATAGRGLRAFGGTPNPLAQYIPHLLVLSAVTLVLF
                     VFWYRVSRDPYIPKRLFTFQTFRASIGTNFLLGTVMMTGMVNVPILVALLETNADTAT
                     RSALLLAPFTATIAVFSLVSARLLATWDADTITRTGLAIALVGNLTIYPLLEYAPYEW
                     MALGLGIAGTGIGLALTPLSTSALESTTERNRGAAASTLLVARLLGMTVGMSLLTSLG
                     VQRLQVLTGRLEPIVRAEGESTAEFLVRQQQFIIEEGLPLGVQVVQETFLAAAVLTLI
                     ALVPARYLSSRGGDEALAGQLSES"
     gene            complement(11794..12465)
                     /gene="rpe"
                     /locus_tag="VGR22_03850"
     CDS             complement(11794..12465)
                     /gene="rpe"
                     /locus_tag="VGR22_03850"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEV2127733.1"
                     /translation="MRQPPIIGPSVLSADFLRYGKALEDATIGGADYIHFDVMDGAFV
                     PNLSIGFPILDATLRGVSLPVDVHLMIENPDRWAPEFAARGVDQVTFHIEASNHAHRV
                     ITAVNEAGAKAGLTLNPSTPLVAIEEMIPFISNLLILTINPGFGGQKLIPEMRDKVRR
                     ARALIDTINPACRLQVDGGVDVDTIPGLYAAGADSFVAGSAIYNDKASVADNIAALRA
                     QLSES"
     gene            12619..13368
                     /locus_tag="VGR22_03855"
     CDS             12619..13368
                     /locus_tag="VGR22_03855"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HEV2127734.1"
                     /translation="MKLLDFLERSVERVIQGPVDSIFRQEIQPAEIERRLERAMLDNR
                     RRASGANIMPNAFVVQLSPEDYDTIEPYRASLVRRLESWLTERADQNDGTLLDRIQIE
                     IEESDRARRRHPVIEATITDISASLVGGARHRPSPAAVQQTQTYHIASPAGATCSFRL
                     LTGPNAGATFAIPIGQSRLGRSSEADIRLDASDVSRNHLRVERQGHQVVITDLDSTNG
                     TRVNGEPVQRATLRDGDEILVGAQALRFLTS"
     gene            13400..13876
                     /locus_tag="VGR22_03860"
     CDS             13400..13876
                     /locus_tag="VGR22_03860"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEV2127735.1"
                     /translation="MPEFESAFLTFEWVMLLLRIAFIGLIYVFLYQIARVALREMVAI
                     GYAVGQQERRSGARRMTAALVVIEPAESSLGMDERIPLSTYTTIGRAAGNALVIDDSF
                     TSSNHAEIVREDDRWLVRDLGSTNGTWVNGRRIHGQTWIEPGDDIAFGNVVVTFTG"
     gene            13909..14745
                     /locus_tag="VGR22_03865"
     CDS             13909..14745
                     /locus_tag="VGR22_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV2127736.1"
                     /translation="MKPSASAYQIPGKLSLVLPAHNEEPNIEIVVRDALEELPEYAPD
                     IEIIVVNDGSRDRTGKIIDRLHAEDPRVVPLHHRRNRGYGGALRSGFEASTGDFVMFM
                     DADRQFDIHDIRLLAPFVDLFDIVAGFRMERSDPIHRRVNAEIFNIAIRVLLGVHMRD
                     LDCAFKIFRGEQIRSLRLTTSGALINAEMQAKLRRQGATLVQVGVPHHPRVAGQATGG
                     SLRVILHAMRETMSLWLRMHRYQPPEDAKEPRPYYPLGDVLIASGTAIAATIVGRMRR
                     RI"
     gene            complement(14991..16433)
                     /locus_tag="VGR22_03870"
     CDS             complement(14991..16433)
                     /locus_tag="VGR22_03870"
                     /inference="COORDINATES: protein motif:HMM:NF016354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HEV2127737.1"
                     /translation="MPVVSELSHRRVDLVPTAPGTVEAVAPVSLAMELGIEEGDRIVG
                     VNGLPLVDALDFQYQAQSTEVVLDVDRNGSLLRYELELEGDEFWGITFADPTFDGVRV
                     CENACPFCFIKQIPKGMRRSLYVMDDDFRYSMLYGSFVTLTNLCEEDWQRIEEQHISP
                     MHVSVHATNPELRVALVGNPKAGKILDDLQRLEDAGIDFHAQLVLVPGVNDGEEMDRS
                     LRDLARFERLRSIAAVPVGLSRHGQDRQSKQIRLSRTCMRRLPGASQERIHVRRYETE
                     EARAVIRQAEVWQERFRQERGETFFHLGDEFYLMVGEEVPGSDHYDGFPQIEDGIGIT
                     RYFLEGVDGYLRRARPGVLRHQQGIVACGTLVGDTMHEAVERFNEVTGSELTVMPVEN
                     RFLGSEINVSGLLSGQDLVSGLGDVPGDAPVFVTRKMISDRNGFLLDDQRIEDVVSQL
                     GRPVVPAETMSEVAAWLRRNRSGAAVRDVA"
     gene            complement(16735..17694)
                     /gene="prmA"
                     /locus_tag="VGR22_03875"
     CDS             complement(16735..17694)
                     /gene="prmA"
                     /locus_tag="VGR22_03875"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEV2127738.1"
                     /translation="MSGDSESVNAASSQPERTWYELSIDVHAEAVEPVSELLARYGFN
                     EGVAIDEPYRQDDDGENLEIDVEKPFTVRTWVADSDYDPSLTEDIRSALWHLGMMRPV
                     SELRVSTLAEEDWANAWKEHFKVHRIGERVVIRPPWLEYEPQGDEVVVELDPGMAFGT
                     GLHPSTRLSMLGVEEMIEQGDRVLDVGTGSGILAIAALKLGAAHADAVDVETVAVRAT
                     EENARRNGVADRVDVRLGSVGLGEPFQGVYDVVLANIIARVLIELADGIVAATKPGGA
                     MVLAGIIEEREAELVAAFEGQGATLVNRRQHEDWVSLVFRRFV"
     gene            complement(17691..18668)
                     /locus_tag="VGR22_03880"
     CDS             complement(17691..18668)
                     /locus_tag="VGR22_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEV2127739.1"
                     /translation="MDVILPVAGLGTRLRPHTWSKPKPLVPVAGKPMLEHVLDRVVVE
                     NPEKLVFITGYRGDQIEEWARATYDISLEFVEQPEMLGQTDAIVRTRDAVKDDALILF
                     PDMLFEADFSGLPDLGVDGVAYTKEIDDPSAYGVAVLNDKGNISRLVEKPSEPVSNLA
                     VIGIYYIRHMADLYDAIDRQIEQQLMTKGEYFLADALQIMIDDGKSFVTRNVPVWEDC
                     GNSEALLQTNRYLLDNGSAAERDLAGGVVIQPSVVHAAADIRGSIVGPYASIHAGATI
                     RNSVITDAIVQENAIIENAVVDHSMIGEKAEIHGTTNRLDIGDNAKVTL"
     gene            complement(18721..19215)
                     /locus_tag="VGR22_03885"
     CDS             complement(18721..19215)
                     /locus_tag="VGR22_03885"
                     /inference="COORDINATES: protein motif:HMM:NF017936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV2127740.1"
                     /translation="MAGEATRLGAEEVIRQLQLAPLEGEGGFFHQTWVSLARSGCPHP
                     RGTVVLYLITPEKWSALHRLRFDEDFHFYLGDSCEMVVAREGEGLRRVRLGPDLRAAE
                     PVQHVVPAGWWQGTQLAPGGEWALLGITMAPRFRPEAFVLATPSVLEEFSAGDRERLL
                     PYLP"
BASE COUNT         3640 a         6777 c         6137 g         3414 t
ORIGIN      
        1 tagccagaca acatcgcgta ggggccgatc ttgtgtcggc cggtctatcg aagccgtcgg
       61 gtcagggcat tgacatcaac gacgaaatcc acccacgatg aggcggtccc atgcccagcg
      121 catcaccgct cgaccaggac tacggcagga tcgatcgtcc cggatacgcc gccgtctccg
      181 ccagctcctc ctcgatgcgc agcagctggt tgtacttcgc cacgcgatcg actcgcgacg
      241 gcgcgccggt cttgatctcg ccggcattgg tcgccaccgc caagtcggca ataaaagtat
      301 ccgccgtctc gccggagcga tgcgagatga ccgcccggaa gccagcacga tgtgccatct
      361 cgatcgcctc cagcgtctcg gtcagcgtgc caatctggtt gagcttgacc aagatcgcgt
      421 tccctgcccc ccgctcgatg ccggtgccga ggcgctcggt gttggtcaca aagagatcgt
      481 cgccaacaag ctggcaccag ttgcccacgc gctccgtcag gtttgcccag gcatcccagt
      541 catcctcggc caggccgtct tcgatcgaga cgatgggata cttgtcgacc cactccgccc
      601 agaaatcgac catttcgtcc gacgagagcg tgcgcccctc cccggcgaga ttgtactggc
      661 catcctcgta gaactcggtc gccgcagggt cgagcgcgat caccagatcc tcacccgccg
      721 tgtagccggc acgctcgatc gcctccagca ccacctgcac ggccgcctcg ttcgactcca
      781 ggctcggcgc gtagcccccc tcgtcaccga cgtgcgtgct caagtcccgc tcggagagca
      841 ccttcttgag cgtgtggaac acctcggtgc ccatccgaag cgcctcggag aaggtcttgg
      901 cgccgaccgg catcaccatg aactcctgca tatcgacact ggagccctct gcatgcttgc
      961 cgccgttgag gatgttcatc atcggcaccg gcagggttcg ggcattcggg ccgccaagat
     1021 agcggtagag cggcaggccg agctcctccg ccgccgcgcg tgcgaccgcc atggatatac
     1081 ccagcagcgc gttggcgccg agccgcccct tgttggaggt gccatcgacc tccttcatga
     1141 gcaggtcgat ccccacttgg tcgagcgggt ccatgccgac cacgacctcg gcaagctcct
     1201 cgttgacgtt ctcgacggca gtcagcacgc cctttccatc gtagcggccc ttgtcaccgt
     1261 cccgcagctc gaccgcctcg taggcaccag tcgatgcacc ggacggcacc gcggcgttgc
     1321 ccaccgcgcc gctggcaaga aaaacctcga cttcgacagt cggattgccg cgtgagtcga
     1381 gaatctcccg cgcgtgcacg gcttcgatga cacgatcgat cattgtctcg cctgctcctc
     1441 gtcttgcccg aacgccatcg tccagcgcgg ttctgcggtc agtataggcg gcaaccgtac
     1501 gcaccatcag ccgcgctggc cgtatactgg caggcgaaag ctgaagagca acgactgtga
     1561 cggaacgagg cctgaggtgc ctcgatcggc cagggagact cgccggcgac tggaagcggg
     1621 tcacgatcga gtcagaccgt tcactcccga gtcggcagcg tcgcgcgacc cgcgcggcaa
     1681 cgccgggctg gtcccccgta gcaggaccga atgattctga ggtgaatcat gagcgcgccg
     1741 gctggtaacc agttggtgaa gcagggtggt accgcgtggc aacccctcgt ccctgccggg
     1801 gggtttttgc atgcccgaga cacacttgtg gtgattcaag gaacgacgat ggcgacaacg
     1861 acacacggac tctcggaaaa gatcgaccgg gtacgtgccg aggcaacaca gcaactggag
     1921 cagatagcct cgccggaggc cgtgcaggag tgggagcgca cgtggatcgg cccgaagggc
     1981 gagatcacgg cgctgctccg ctcgctcggc tccctcccga aggaggagcg tccggacgcc
     2041 ggccgcgaga cgcaggcgct ccgaaaggag ctggaagcgg cgttcgcacc ggtcaaggag
     2101 cgcatggagc agcaggcgct ggaggaacgg ctcgcctccg aggccgtcga tgtcaccctc
     2161 cccggtatcg agccgcgcat cggcgcgatc cacccaatct cgacgatgat cgatgagttg
     2221 atcgagatct tcgcccagat gggcttccag gcggtgttcg gtccggaggt cgaaactacg
     2281 cgctacaact tcgacctgct caacatcccc gccgaccacc ccgcccgcga cgtttgggac
     2341 acggtcatcg tcgacgaccc aagtggcgag atcgtgctgc gcacccacac ctcgccgatg
     2401 caggccagga cgatggagcg aatcgatccg ccggtcaggg tgattgtgcc cggcgcggcc
     2461 taccgctacg aggcgcagga cgcctcgcac gagtggcagc tcacccagct ggagggcctg
     2521 gcagtcgacg agcacatcac catgactgac ctgaaggggg tgctgcagga gatggcgcgc
     2581 cagctgttcg ggccgcagcg caaggtccga ttacgctgtg acttcttccc cttcgtcgag
     2641 ccgggcgtcg acttcgcggt cgactgcgcg atatgcaacg gcaagggttg ccgggtctgc
     2701 aagcacaccg gctggctgga gatgggcggc gccggcatgg tgcatccgaa cgttctccgc
     2761 ggtgtcgggt acgacccgga gaagtacacc ggcttcgcgt tcgggctcgg tatcgagcgg
     2821 ctcgtgatga tgaagtacgg cgtcgatgac atccgtgcct tcgccagcaa cgacctgcgc
     2881 ttcctgaaga agttcgccgc cgcggcagta tagcggtcgt tgctgcgacg cctctaggcg
     2941 ggcaggcact cgaccggccc gtacgggagg atgcgatcgc gaagcgaccg cggggcgcac
     3001 acagggtccg cccctacgcg gaccgacatc agttcggccc ccacggagac accaccgatg
     3061 aaagtcccgt ttcgatggct gcaggaatat gtcgatatcg acatcagccc cgaggagttc
     3121 gcccagcgca tgacgatggc cggtctcgag gccgagcaga tcgactatgt cggtgacggc
     3181 tgggacaagg tctatgtcgc cgaggtgttg aatctctcgc cgcaccccga cgccgaccgc
     3241 ctcgtgctgg ccgacgtcgc cgccggtgag caccgcctca ctgtcgtcac cggcgcgccg
     3301 aatatcgaga agagccagaa ggttgtcctg gctctcgcgg gtgcccgcct gatcgacgcc
     3361 tacgctgacg aactgaagta caagacgctc aagccgggca agattcgcgg catcacctcc
     3421 gaggggatgg tctgcagcga aaaggaactc ggcctcagtg acgagcacga gggcatcctc
     3481 gtcctcgacg atgatgcgcc ggtcggcgcg ccactcgccg actatctcgg cgacgccatc
     3541 atcgagttcg agatcacccc gaacctcgtg cacgcgttct cgatggtcgg catcgcccgc
     3601 gaggccgccg cgctgcttga gaaatcgtac cgggcaccga aggaggcgga tctctcgaca
     3661 tccccttccc gcgacgacct cgtcacaatc gaggcgccgg agctcgccag ccgctacgtc
     3721 ggcgtggtaa tcgagggcat ctcgatcgag ccgtcgccct cgtggatggc ccagcgtctc
     3781 aatcgcgccg gcgtccggcc cgtgaacaac atcgtcgacg tgacgaacta cgtgctgctc
     3841 gagatgggcc agccactcca cgcgttcgat ctcaagacca tcgagaccgg gcacatcgtc
     3901 gtgcggcgtg gcaaggcgaa cgagacgatc gaaaccctcg accaccgcac ccgcgaggtc
     3961 gacgaggaca ccctgctcat aaccgacggc aaccgcccgg tcggcatcgc cggcgtgatg
     4021 ggaggtgtca actccgaggt caccgacgac accactgcga tcgtgctgga gtccgcgacc
     4081 ttcggcatgg tcaacatccg gcacacctcg cgaaacctga agctccgcac cgacgcctct
     4141 gcgcgcttcg agcgcggtct cgatccggaa ctggccattg cggcggcaca gcgcgccgtt
     4201 cacctcatcc tggagctctg tccgaacgcg agggttgtcg agcacgacga cacgtatcca
     4261 aatccggtcg agccgcgaca ggtcgccctc cccttctccc gcttcgagcg cgtgctcggc
     4321 atcgaggtgg accgggacca ggcgctcggt gtactcgagc gtctggagct gcagccgcgc
     4381 ctcgacgatg caacaggagt gctcaccgtt accgtcccga catggcgcag cgatatcacc
     4441 atccccgagg acgtcgtcga agaggccgcc cgcatgatcg ggtatcacct gctccccgcg
     4501 acgctcccga ccggcaccac gcccgaggtc gagcgcgacg ggatgttcct tctcgagcgc
     4561 aaggttcgga acatgcttgc cggcggcggg atgtacgagg ggcgatccta cgtcacgctc
     4621 tcggaagagg aggtcgaccg ttggtcgagc gggacctctc tctctctgac ggcggaggtc
     4681 ccgacggacg cagccgtgca gttgctcaac ccggtgaaca cggagcgccc gctgcttcgt
     4741 ccgagcctcc ttccatccct gctgcctgcc gtcggggata acctcaaaca ccagcagagc
     4801 gtgcgcctct tcgaggtcgc gcacgtctac gcctccaacg gccccggcca gctgccgaac
     4861 gagacgtcta atgtcgcagg ggtgctcgcc gggcggcgtg aggagctcga ccgcttcaac
     4921 cccgcctcag acgagatgga tttctgggac gccaagggag tggtcgacgc cctcggcgcg
     4981 cgcctcgggt tgacgctctc gtacgagcgc accacccacc cggccttcca cccagggcgg
     5041 acggcgtccg tgctggccga gggcacgcgc ctaggcatcg taggcgaaat ccacccgaac
     5101 ctcaccgacg aatatggcgt ggaggcgcgc actgccgcct tcgagctgag cctcgatgcg
     5161 ctgcagggtg cgatgcccga cattcgtggc atccaggtaa ccaccgacca gtacctgcct
     5221 gtgcagcagg acttctccat cgtggtcgac gccgataccg ccgcggccga cgtcgaggcg
     5281 tcgctcatgc agggggccgg cccgctggcg accgatctcg tgctcttcga cgtctatcag
     5341 ggcgacaaca tcgaagcggg caagaagagc ctcacgttcc gcgtgacctt caccgcgccg
     5401 aaccgcgcgc tcaccgacaa ggaactcgag aaggtgcgga agaagatcga gaagaccctc
     5461 cagcgcggca ccgcggcaac cctgcgggct gtagaaagct gaccatggcg acatcgacca
     5521 ttacgatccg gcggatcgag aacttcgatg aggcgctggc ggagctcacc cgccgaggat
     5581 cgttcctgga ccctcagctg tccgatcgga ttcgcgaggg catccggcag gtcttcggcg
     5641 aggacctcag cgcccgggag gtcgtcgagc gcatcgtcac ggacgtgcga tcgcagggcg
     5701 acgccgcgct ccggcgctac accgaggcgt tcgacggcca gacgcgcgat tcgttcgagg
     5761 tgccgaagga cgattggcgg tccgcacggg agagactcga tgcggacgtc gtcgccgcca
     5821 tggagacagc cgttggccga atccaggcct tccatgagcg ccagccgggc cggtcgtggc
     5881 tccatcccac accccaaggc actttcggcc agatcgtccg gccgctggag cgcgtgggac
     5941 tctacacgcc cggcggcacg gcggcgtatc catcgtcgct actcatgtcc gcagttcccg
     6001 cgcgggtcgc cggcgtgcag gaggtggtga tcgcgacccc gcccgcgcgt gacggtcgca
     6061 tcgccccgac gatcctggcc gcagcggaca tcgccgccgc cgaccgggtc ttcgccatgg
     6121 gcggcgctca ggcgatcgcc gcacttgcgt tcggaacgga aaccgtgccg catgtcgaca
     6181 agatcttcgg accaggcaac atctttgtga cgctcgccaa gcagcgcgtc tatggcgtgg
     6241 tcgacctcga ccagctcgcc ggtccgaccg agaccctgct gatcgccgac gagacggccg
     6301 atgtggagct ggtcgccgcc gacctgattg cccagtcgga gcacgacacc atcgccagcg
     6361 cactgctgat cacaacgagt gtgggcctcg ccgatgccgt acccaacgag atcgagcggc
     6421 agattgccga gctcgagcgt gcctacatca tccgtgcatc aatgtcgacc aacgggcgca
     6481 tcatcgtgac cggaacgctg gatgaggcag tccggctcgc gaacgcctac gcgccggagc
     6541 atctctgcct gctgaccgcc cgcccgtggg acctggtgcc gaacgtcgag aacgccggcg
     6601 gcatcttcgt cggcgaggac agcccggagg cgctcggcga ctacaccgcc ggcccgagcc
     6661 acgtcatgcc gaccggtggc accgcccggt tctcgtcccc ggtccacgtc ggggaattcc
     6721 agaaggtgat ctcgctgctg gccgcctcgc cgcaggccgt tgacgagctg ggtgaggcca
     6781 cgatcgaact cgcgaatgct gagggcctga ccgggcacgc ctccgccatc cgccgccgcc
     6841 tagaaaagcg caagaagccg tagagtcctt cgtgcgatca cgagacagcc agccgagtag
     6901 ccccgcccac gtgcaaggcg ggtctcgtct tcatggtcag gtaccgtcca ggacccgagt
     6961 gagtcgagga tggtaagaac cgcctcatcc tgacggcgtg agctgacgta gcgcgctgag
     7021 caggcgatcc tccagcgttg cgtccgggga caggtccacc cgcgacgcag cgccaaccgc
     7081 ttcgacacgc gaatacccca agccctccag cgcctcgatc agatcgtcgt cctgcatccg
     7141 gacctgcccc gtcagcggcg tgccagtcgg ctccgggatc ttgccccgca gatcgagcag
     7201 gatacgcgac gccgtcttct tgccgagtcc tgggacggtg gacaggagca tgacgtcttc
     7261 gcggtggaca atcccggcca tctcgtcggc gctgaacttc gacatcaagg caagggccac
     7321 cttcggcccg accccgttca gcccaatcag cacccgaaac cactccacat ccgacaccga
     7381 ctcgaagccg tagagcgtca ttgagtcctc gcgcacgacc atgtgtgtct ccaggtcgag
     7441 ccgctgcccg gtctccaacc caccgccctg ggtcatgctg gcgggcacat ggacccggta
     7501 gagcactccg ttcacatcga gaatgaccgc tcccggctcc agccgtgcca ccgttcccct
     7561 gagccctgcg atcacgtcgc cacctcccgc acgcgcaggc catagacccg cgcagcgttg
     7621 gcacccacca cggcgtccgc cgtctcatcg tcaagtatcg accgcgcctt cgcgagaaac
     7681 cgtcgctggc ccaacaccgg atagtccgag ccccacacaa cgcgcctcgg tccgacaatg
     7741 cgattgacga cgtcgaacac ctcaggccga tagaggtagg tcgaggcggc cgtgtcgtag
     7801 gtgacgttgc gcagcgctag accgacctcc ggcatcaact cgtagaacgg cagcccgccg
     7861 ccccagtgag ccgcgacgaa ccgcaaatcc gggtgctgct cgaggaaggc caggatcttc
     7921 ggtggcgtag ccgttccctt gcccggatag tcgtggccca ctggctcgga aacatgtgcc
     7981 atgatcggga aatcaagggt cgtggcaatc tctgccagct cgcgcacgtc cccttcccgc
     8041 tcccacgcaa agccctgggc atcggcgttg agctcgccga tcccgacggc gcccaatcca
     8101 cgcacccgct ccaattcctg gggagcgctg gcatcagcag ggctgacgat cgcgagccat
     8161 gccagccgcg ggctcgttgc agcgacctcc gcaagccagt cgttgtgcgc acggcaatgg
     8221 cccggatcga cccaaggcca tgcggcgatc acggccatgc tgacacccgc ctcgtccatg
     8281 ctctcgagca ggtgctcggc ttgggggaag cgctccaccg gccaggcagc gtgtgtgacg
     8341 ccgaaccacg catcacgctc gcagtagtgg tccttgccgg cgatcacatc cggcgggaac
     8401 aggtggacat gggcatcgac gacgggactg ggcatgatcg agcgctcggg tactagagga
     8461 ggaacaccag ggcgagcgct gcaatcgcga tcaccacaag caccaacacc accgtgacga
     8521 cgatcgcgtt gccctgcggc cgctgctcgg caatcgtcgt ctccagttgc tggcgctgct
     8581 cagtagcgcg agacggcttg gcgatcaaca cgacggccga gacattgtcg gtcgtgcctc
     8641 gctgctcggc cagcgacgcg agagacatcg cgagcgtcga agggtcgccg ccggcgagct
     8701 gcgcggcgta atccctctgc tccacgacgt cgtgaaagcc gtccgaacac agcagcagcc
     8761 gatcctccgg gatcagcgag agctcgtaaa tcgcagggag cttgcggtcg agccgctcag
     8821 tggtgccgac ggcagccgtc agcacgttcc gctgtggact ctggcgttcg gtgccagcct
     8881 cgatttctcc gcgttcgacc tgctcggcaa ccagcgagtg gtcctgcgtc acctgcagca
     8941 cctggctgcc tcgaatgaga taggcacggc tgtcaccgac gttggcgatc gtgagataag
     9001 atccgttgat gaccgctgcg acgagcgttg ttcccatgtg cgtcgcgctc tgctggccgc
     9061 cctcggccca gatgcggtcg ttggcctgac ggtaggcgcg cttcagctcc aatgcaacat
     9121 cgtctgggag ggggccagcg aattgctgct gcagcgtctc aatcccgagc ctactggcga
     9181 cctcaccgcc ctcgtgccca cccataccat cagctacggc gagcagccat gcgttgccac
     9241 cgtcaccgat ctgcaccgcc agcacggcat cctcgttgtg gtcgcggcct ccggcgcggc
     9301 tcgcgctgcc gacctcgacc tcgaccgcgc gctgttcggt caataccatc tgatccatca
     9361 ctcccttgtc atgcggcgcg tcgtcactgg tcctccgaag ctccaggaca catcacttgc
     9421 cagtgctaga ctaccagcca gaccggatcc tcgtcccctt tcccgcacac tggtggagaa
     9481 tcatgcgact tctcggcatt ctgaccatca ccttgctctt gcttgcgaca tcatcgctgg
     9541 ctcccgtcct agcgcagccg ggtggtaccg ccacccccgc ctccgagtcc gaagcgcagg
     9601 ccctgctgcg caaggcaagc gagcggctcg cggaaacgga aaccgtacgg ttcaacctcg
     9661 aggtcgaggg agacacgtac atcgatgagg agcaggacat ccggctcatc aacgcgcgcg
     9721 gcaacctcgc tcgcccggac tcagtcgacg tcgagtttca agttgagctg ttggggacac
     9781 agacggtgtc tatccgcatg atctccatcg gcgacgagtc ctggacgact gacctcctca
     9841 caggtgagtg ggccccgtct cccgaggagt ttggctataa cccgacagtc ctcttcgaca
     9901 accagaaggg actcgggccg gtcgccggcc gcctcgtcga cccagagatc gtggacaagg
     9961 tggagatcgg cggccgcgag gcctggaagg tggtcggcac ggtcgacgag gaggtcatcg
    10021 agccgatcac ctccggcacc atggagggcg aggaaatcac cgtcaccctg tggctggata
    10081 ccgagacgta cgacatgctc cggatcaggg ttgaggaacc gaaggatatc gccaaggaga
    10141 atccggcgac atgggtcctg agcctcaccg gccacgacag tgacctgacc atccagcggc
    10201 cggacatcga cgagtgacgc aacgctctgc acaggcgagc ccttggcgag acccactctc
    10261 gctcatcatg atcttcgcgg tcttcgtggg cgcaatcgat ctcaccgtcg tcgcgaccat
    10321 cctgccgcgg atgatcaccg atctgggtat caataccgcc gacatcgacc gatacatctg
    10381 ggtggtcaac agctacctca tcggctacgt cgtctcgatc ccgctcttcg gccgtctctc
    10441 ggacatcctc gggctgcgca acgcgctggt cagctccagt ctcttcttca tcgtcggttc
    10501 ctggtggtgc gcacaggcgg aatcgctgcg ggatctcgtc atcggccgcg ccgtgcaggg
    10561 gttcggcgcc ggagcgttgc tcccgctggc tttggcagcc gcagcagcgt cgttcacagg
    10621 ggcaaaacga gcgaatgcgg tcggcgcgat cggcgcaagc gacgagatcg gctgggtctc
    10681 ggggcccgcc tacggcgcgc tggtcgtcaa ccaactccag atcatcgacg aaccctggcg
    10741 gctcatcttc tgggtgaaca tccccatcgc cctggcactc gtcggcctcg cgtggaggaa
    10801 actcggtggg ctccagccgc cacgctacca ccacccaggc agcttcctgc agcggcttga
    10861 cctgcccggc ttcgtgctga tctcgacctt cctcgtggcg atcaacctgg cactcgcctc
    10921 gggcggcgag atcggggcaa cagccggccg cggactccgc gcctttggcg gcacgccgaa
    10981 tccactggcg cagtacatcc cgcacctgct cgtcctctcc gccgtcacgc tggtgctgtt
    11041 cgtcttctgg taccgggtca gcagggaccc ctacatcccg aagcgactct tcacgttcca
    11101 gacattccgc gcatcgattg gcaccaactt cctgctggga acagtcatga tgaccggcat
    11161 ggtcaacgtg cctatcctcg tcgcgctgct tgaaacgaac gcggacacgg ccacccgcag
    11221 cgccctgctg ctcgccccct tcaccgctac gatcgcggtc ttctcgctgg taagcgccag
    11281 attgctcgcc acttgggatg ctgacacgat cacgcgaacc gggttggcaa tcgcgcttgt
    11341 tggcaacctg acaatctatc cgctgctgga gtacgcccca tacgagtgga tggcgcttgg
    11401 actcgggatc gccggaaccg gtatcgggct tgccctgacc ccgctgagca caagcgcgct
    11461 cgagtcgacc actgagcgaa accggggcgc agcggcgtca acgctgcttg tcgcgagact
    11521 cctggggatg acggtcggga tgtcgctgct gacgtcgctc ggtgtccagc gtctccaggt
    11581 gctgaccggg cgtctcgagc cgattgttcg tgcggaggga gaatccaccg ccgagttcct
    11641 ggtccgccag cagcagttca tcatcgagga aggactgcca ctcggcgtgc aggtcgtcca
    11701 ggagacgttt ctggcggcgg ctgtactcac gctgatcgcg ctcgtccccg cacgctatct
    11761 ctcgagccga ggcggtgatg aagccctcgc gggtcaactc tcggagagtt gagcgcgcag
    11821 cgcggcgatg ttgtcggcga cactcgcctt gtcgttgtag atcgcgctgc cagccacgaa
    11881 gctgtccgct cccgcggcgt agagccccgg aatcgtgtcg acgtcaacac cgccatcgac
    11941 ctgcagacgg cacgccgggt tgattgtgtc gatcagggct cgcgcccgcc gaaccttgtc
    12001 ccgcatctca gggatcagct tctggccacc aaacccggga ttgatcgtca ggatcagcag
    12061 gttcgaaatg aacgggatca tctcctcgat cgccaccagc ggcgtcgagg ggttgagcgt
    12121 caggcccgcc ttcgcccccg cctcattgac cgccgtgatc acccgatgcg catggttgga
    12181 cgcctcgatg tggaacgtga cttggtcgac ccccctcgcc gcgaactccg gcgcccagcg
    12241 gtccgggttt tcgatcatga ggtgcacgtc gaccgggaga gatactcccc gcaaggtggc
    12301 gtcgaggatc gggaagccga ttgagaggtt cggcacgaag gcgccatcca tcacgtcgaa
    12361 atggatgtaa tcggcaccac cgattgtagc gtcctcgagc gccttcccat agcgcagaaa
    12421 atcggcggag agcacggacg gcccgatgat tggtggctgc cgcatggtcg tgaacatcct
    12481 tcccggttcg caggcggcac ggccgcccgg tcggcattgt cccctggccg gatacggtaa
    12541 tctgtatggg cagggagagc cacctagctc gcggggcagc atccgcccaa gccacccacc
    12601 gaagggaacg agccgcacgt gaagttgctc gattttctgg aacgatctgt cgaacgcgtc
    12661 atccaggggc ccgtggactc tatcttccgg caggagatcc agccggcgga gatcgagcgc
    12721 cgtcttgagc gcgccatgct tgacaaccgc cgccgggctt ctggcgcgaa catcatgccg
    12781 aacgcctttg tcgtacagct cagccctgaa gactatgaca ctatcgaacc ctacagggcg
    12841 tcgctcgtgc ggcgcctgga gtcgtggttg accgagcggg cagaccagaa cgacggcacg
    12901 ctgctcgacc gcatccagat cgagatcgag gagagcgacc gcgcccgccg gcggcatcct
    12961 gtaatcgagg cgacgatcac cgacatcagc gcgagcctcg tgggaggcgc tcgtcaccgg
    13021 ccgagcccag ccgctgtcca gcagacccag acctaccaca tcgcgagtcc agcaggcgca
    13081 acctgctcgt tccggctgct gaccggcccg aatgccggcg caacgttcgc gataccgatc
    13141 ggccagtcga ggctcgggcg ctcctcggaa gcggacatcc ggctcgatgc cagcgatgtc
    13201 tcccgcaacc atctgcgggt cgaacggcag ggccaccagg tggtcatcac cgacctcgac
    13261 agtaccaacg gcacccgcgt caacggcgaa cccgtacagc gcgccaccct ccgcgacgga
    13321 gacgagatcc tcgtgggtgc gcaggcgctg cgattcctga cgtcctaaca cggtcgcgaa
    13381 tccccccaag gaacgtcgaa tgccggaatt cgagagcgcc tttctgacct tcgagtgggt
    13441 gatgttgctg ctgcgcatcg cgttcatcgg gctgatttac gtcttcctct accagatcgc
    13501 ccgcgtagcg cttcgggaga tggttgcgat cggttacgcg gtcggacagc aggagcggcg
    13561 atcaggcgcg cgccgaatga ccgccgcgct cgtggtgatc gaacccgcag agagctcgct
    13621 cggcatggac gagcgcattc cgctctcgac ctacacgacg atcggtcgcg cagctggcaa
    13681 cgcgcttgtc atcgacgata gcttcacatc ctccaaccac gccgagatcg tccgtgagga
    13741 tgaccgctgg ctcgttcgcg acctgggcag caccaacggc acgtgggtga acgggcgccg
    13801 tattcatggc caaacatgga tcgagccggg cgacgatatc gccttcggca atgtggtggt
    13861 aaccttcacc gggtagtttc tcgaatacga tcatcgcctg gagcgttcat gaagccgtcc
    13921 gcatccgcgt atcagattcc cggtaagcta agcctcgtgc ttcccgccca caacgaggag
    13981 ccgaacatcg agatcgtggt tcgggacgcg ctcgaggagc tcccggaata cgcgcccgat
    14041 atcgagatca tcgtcgtcaa cgacggcagt cgcgaccgca ccggcaagat catcgaccgg
    14101 ctgcacgccg aagacccgcg ggtcgtcccg ctgcatcacc gcaggaaccg gggctacggc
    14161 ggcgcgctcc gatccggctt cgaggcctcg accggcgatt tcgtgatgtt catggatgcc
    14221 gatcggcagt tcgatatcca cgatatccga ctgctcgcgc cattcgtcga cctcttcgac
    14281 atcgtcgccg gtttccgaat ggagcgctcc gacccgatcc accggcgcgt caacgccgag
    14341 atcttcaaca tcgcgatccg tgtgctcctc ggcgtccaca tgcgcgatct cgattgcgca
    14401 ttcaaaatct tccggggtga gcagatccgc agcctgcgcc tcacgacgtc cggcgcgctc
    14461 atcaatgccg agatgcaggc caagctgcgg cggcagggcg cgacactggt gcaggtcggc
    14521 gtgccgcacc acccccgagt ggctggccag gcgacgggag gcagcctcag agtcatcctg
    14581 catgcgatgc gggagacgat gagcctctgg ctgcggatgc atcgttacca gccgcctgag
    14641 gacgccaagg aaccgaggcc ctactatccg ctcggcgacg tgttgatcgc cagcgggacg
    14701 gcgattgcgg ccaccatcgt cggcagaatg cgccgccgca tatgacgaaa cccgccgggg
    14761 agctccccgg cgggtttcgt catccaaatg gatcgtaggg gaggacttga ccgcgttcac
    14821 cgaccgtcgt ggcagacgat gcgcacgcgg ccaatgtcct cccgtatcgt gcacggcgtg
    14881 tgcctgcaat ggtaggacga cattggcggc gcggaacgtt gatagcttcg caccttggcc
    14941 ggcgccgcca atgtcgtctc ctacagctac agccgtgctg tgaggacgaa tcaggccaca
    15001 tcccggacgg cggcgccaga gcggttgcgg cgcaaccaag ccgcgacctc gctcatcgtc
    15061 tcagccggga ccaccggacg cccgagctgg ctgacgacat cctcgatgcg ttggtcgtcc
    15121 agcaggaagc cgttccggtc ggaaatcatc ttccgcgtga cgaagactgg cgcgtcgcca
    15181 ggcacgtcac ccagtcctga gaccagatcc tgcccggaga gcagtccgga gacgttgatc
    15241 tcgcttccga ggaacctgtt ctccaccggc atcacggtca actcggaacc ggtaacctcg
    15301 ttgaagcgct ccactgcttc atgcatggtg tcgcccacca gcgtgccgca tgcgacgatg
    15361 ccctgctgat gccggagcac gccgggccgc gcacgccgaa ggtagccatc gacgccctcg
    15421 aggaagtacc gggtgatgcc aatgccatcc tcgatctgcg ggaacccgtc gtagtggtcg
    15481 ctgcccggca cctcctcgcc gaccatcagg tagaactcgt cgccgagatg gaagaaggtc
    15541 tccccgcgct cctggcggaa acgctcctgc cagacctccg cctggcggat gaccgctcgc
    15601 gcctcctctg tctcgtagcg gcgaacgtgg atgcgctctt gactggcgcc aggcaaccga
    15661 cgcatgcagg tacgtgacag ccggatctgc ttgctctgcc gatcctgccc atggcggctc
    15721 agcccgactg gcaccgcggc gatcgaccgc agccgctcga accgcgccag gtcgcgcagg
    15781 ctccggtcca tctcctcacc gtcgttgacg ccgggaacga gcaccagctg ggcgtggaaa
    15841 tcgatgccgg catcctccag ccgctgcaga tcgtcgagga tcttgccggc cttgggattg
    15901 ccgaccagcg cgacgcgcag ctccggattg gtggcatgca ccgagacgtg catcgggcta
    15961 atgtgctgct cctcgatgcg ctgccagtcc tcctcgcaga ggttggtgag cgtcacgaac
    16021 gagccgtaga gcatcgagta gcggaaatcg tcgtccatga catagaggga tcgccgcatc
    16081 cccttgggaa tctgcttgat gaagcagaac gggcaggcgt tctcgcagac gcgcaccccg
    16141 tcgaaggtgg gatcggcgaa cgtgatgccc cagaactcgt cgccctcgag ctcgagctcg
    16201 taccgcagga gcgagccatt gcggtcgaca tcgaggacca cctcagttga ctgtgcctgg
    16261 tactggaagt cgagcgcgtc aaccagcggc aagccgttta ccccgacgat ccggtcgccc
    16321 tcctcaatcc caagttccat ggcgagagag actggcgcca ccgcttccac cgttccggga
    16381 gcggtcggaa ccaaatcgac acgtcgatgg gaaagctcac tgacaactgg catcaagaat
    16441 ccttgcggtt cgggcagact gctgaatcgc agaaaattgg aggaatggtt gaagcatacc
    16501 ggcagcctcg aggctgtcgg acaacacata acgctaccac aaatgaagta ttggtgtagt
    16561 gtggagtgaa cagcacaaca cgcccgagga ttaccgtatg gtttggaccg aagcgcctgc
    16621 agggcgcgga gtgaggaatc ccttgccggt caggaatcgg cctccccgca ttaagagatt
    16681 tctcgactcc acggcttcgc cgctccgcac aaaatgacag gtttggtggg gccactatac
    16741 gaaccgacgg aagacgagcg atacccagtc ctcgtgctgt cgacggttga ctagcgtcgc
    16801 accctgcccc tcgaacgcgg cgacgagctc tgcctcgcgc tcctcaatga tcccggcgag
    16861 caccatcgcg ccgcccggct tcgtggcggc aacgatccca tcggcaagct cgatcaggac
    16921 gcgcgcgatg atgttggcca gcacgacatc gtagacgccc tggaacggct cccccagccc
    16981 tacactgccg agccggacgt cgacccgatc ggcgactccg ttgcgccggg cgttctcctc
    17041 ggtggcgcgg acagcgaccg tctcgacgtc cacggcatcg gcatgcgccg cgcctagctt
    17101 caaggcggca atggccagga tcccggaccc ggtgccgaca tcgagtacgc ggtcaccctg
    17161 ctcgatcatc tcttcgacgc ccagcatcga gaggcgcgtc gatgggtgca gtcccgtacc
    17221 gaaggccatg ccggggtcga gctcgaccac cacctcatca ccctgtggct cgtactcgag
    17281 ccacggcggt cggatcacga cgcgttcgcc aatgcggtga accttgaagt gctccttcca
    17341 ggcgttggcc cagtcttcct cggcaagcgt cgacacccgc agctcggaga cgggacgcat
    17401 catgccgagg tgccacagcg cgctgcgaat gtcctcggtg agcgagggat cgtagtcgct
    17461 gtcggccacc cacgtccgaa cggtgaacgg cttctcgaca tcgatctcca gattttcgcc
    17521 gtcgtcgtcc tgcctgtacg gctcgtcgat ggcgacgccc tcgttgaagc cgtatcgggc
    17581 aagcaattcg gagaccggct cgaccgcctc ggcgtggacg tcaatgctca gttcgtacca
    17641 cgtgcgctcc ggctgactcg atgccgcgtt gacggactcg ctgtctccgc tcacagagtc
    17701 accttcgcgt tgtcgccgat atcgagccgg ttcgtggtgc catggatctc tgccttctcg
    17761 cctatcatcg agtgatcgac caccgcgttc tcgataatgg cgttctcctg cacgatcgca
    17821 tcagtgatca cgctgttgcg gatcgtcgca ccggcgtgga tgctcgcgta cggtcccacg
    17881 atcgacccac ggatgtcagc ggcggcgtgc acgaccgatg gctggatgac gaccccacca
    17941 gccaagtcac gctcggcggc gctgccgttg tcgagcagat agcggttcgt ctggagcagc
    18001 gcctcgctgt tgccgcagtc ctcccagacc ggaacattgc gagtcacgaa cgacttgccg
    18061 tcgtcgatca tgatctgcaa cgcgtcggcg aggaagtact cgcccttcgt catgagctgc
    18121 tgctcgatct gccggtcgat ggcgtcgtac aggtcggcca tgtgccggat gtagtagatg
    18181 ccgattaccg caaggttcga gaccggctcg gatggcttct ccaccagccg gctgatgttt
    18241 cccttgtcgt tcaacactgc gacaccgtac gcgctgggat catcgatctc cttggtgtag
    18301 gccacgccgt cgacgccgag atcgggcagg cccgagaagt cggcctcgaa gagcatgtcc
    18361 gggaagagga tgagcgcgtc gtctttgacg gcgtcacgag tgcgcacgat cgcgtccgtc
    18421 tgtccgagca tctccggctg ctcaacgaac tcgagggaga tgtcgtacgt cgcgcgggcc
    18481 cactcctcga tctggtcgcc gcggtagccg gtgataaaga ccagcttctc ggggttctcg
    18541 accaccacgc ggtcgagcac gtgctccagc atcggcttcc cggccacggg cacgagcggc
    18601 ttgggcttgc tccaagtatg cgggcgaaga cgcgtcccga gtccggcgac aggcagaatg
    18661 acatccacgg cagaaagatc ctttcacagg tacgcgaata gcacaatgtc cccaaatctc
    18721 ctacgggaga tagggcagca gccgctctcg gtccccggca ctgaactcct ccagcacgct
    18781 gggggtggca agcacgaaag cctccggacg gaacctgggc gccatggtga tgcccagcaa
    18841 cgcccactcc ccgcccggcg caagttgtgt tccctgccac cacccggcgg ggacgacgtg
    18901 ttggaccggc tctgcggccc gcaggtcagg gccgagccgc acccgtctca gcccctcacc
    18961 ctcccgagcc accaccatct cacaggaatc accgaggtaa aagtggaagt cttcatcgaa
    19021 ccgcaaccgg tgcagcgccg accacttctc aggtgtgatc aggtagagga cgaccgtgcc
    19081 cctcggatgc ggacagccgc ttctggccag cgatacccat gtctggtgga aaaagccgcc
    19141 ctcgccctcc agcggcgcga gctggagctg ccggattacc tcctcggctc ccaggcgtgt
    19201 cgcctcgcct gccatacaat cgccttcaca aatcgcaagc gcagcctaag caatgtaaag
    19261 aggcggcgac ccggatggat cgccgcctct gcctggtacc ccttcctaga cgagtttcga
    19321 acactttgtg tcgctccacc acaagaggtt cgtgctctgt tcgacgctct caaggactgc
    19381 gaaagtgtag ctgtatgatc tccggccggc aacctggctg gcacacggct tccgtggttg
    19441 ttgaattgtc agagttgaga tcgcggaagc gacgggggtt ggatcagcac cttcaatacc
    19501 atctcgggca atctgcccgc attccaatgc gtcacacggg atcgagcatc caaagggcat
    19561 cagaaggcgc atcgggttca ccccgatacc ggcatcgccc tcgcaagctg acgacggcgg
    19621 agatggaagc gatccgaatt ctgtccgctt ccagtagttt gcgcttgttg gctgcgcaat
    19681 atggtgtaag ccacgagacg atccgctccg tcatcctgag caaggactag cctagcatcg
    19741 tgagagttct cgccggatct cgtgcacgtt gttcatgatc cgagattctt tgtcggttgg
    19801 cttggggcaa tcatgcagag gacccgcaca atggctcgcg ccaaagggcc cagaatcact
    19861 ctcagctgca tagtcctcaa taccagcgag cccggtctgt ctccgtccgt ctctactcca
    19921 cgagaaagac gaagatgctg atctgagctc agaagacaac tcaaagca
//
LOCUS       DASYWL010000103         3162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_455418, whole genome shotgun sequence.
ACCESSION   DASYWL010000103 DASYWL010000000
VERSION     DASYWL010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455418"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..1366)
                     /locus_tag="VGR22_03890"
     CDS             complement(35..1366)
                     /locus_tag="VGR22_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127741.1"
                     /translation="MRDTDLWSEAITVLDRNRLAGWTKAAPRLYPHQWSWDSAFIAIG
                     LARFDPSRALEEMRSLIRGQWRNGMIPHIVFNPDVAPGEYFPGPKRWACADFAPDAPE
                     GVDTSGLVQPPVHAIGILRAVDALLQEQRAVRAREFFGPLFDWHRYLLSARDSESSGL
                     VTIVHPWESGMDNSPRWTAAMEAITVDPERLVRYERADLKHVEDPSQRPSKADYDRYL
                     WIVDRLVEAEYRVDAAYQTLPFRMKDVFMSAILVAANEALLRLASIADASLEQVEIIE
                     SWIERGRLGLAEQWNDDRVMCCDRDLVAGRDVTVRTLASFAPVIAGGLTSERRDALLE
                     VWRSEAMVGHPNLRWPLPPSTSPLEPEFSPRTYWRGPVWPVMNWLFAWSLRRSGAPND
                     ADQLARAGLAQVRAIDFAEYAEPFTREPLGSLDQSWTAAVALDWLAHGIDA"
     gene            1478..2866
                     /locus_tag="VGR22_03895"
     CDS             1478..2866
                     /locus_tag="VGR22_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV2127742.1"
                     /translation="MAAWPHIRTDEQTRAVTAWWMRLGTQPDGEIRDALYDLVPLTRD
                     DLALIDTTAFQRLDRVQQLGFVSHIWPGAKHTRFEHSLGVMHLARRSAVRLSQRANGG
                     WVRLDDIRTVAAAALLHDIGHYPFSHAIEELGSPVLPHEAVGRRLIEEGELADVLRDA
                     CRLDPARVASLIHGERSQLPPRDRILFQILSGTLDVDKLDYLPRDARACHVPYGGVDT
                     DRLIGALDLAMIEAVDQEGEDARVVVTPKGISALHSLINARQEMFDNVYWHHTNRTCM
                     VMLLRAVQDALGHERIAPEELWRLDDASLLNRLQAPEMPNTTRQLATALATRDFHKRA
                     LEVSNRAIELYGRLGNLWFDPGARREVECQLAASLSERLEREVSEAAILIDIPKPEKW
                     KSDVWVVYERPPVGMRKQMHWKEVVGVDDADFKRYEEHRRLIRIVTRPELREVVAAEW
                     ESLIYPLLGGVF"
BASE COUNT          530 a         1045 c         1052 g          535 t
ORIGIN      
        1 gatgccgatc gcacgcccgc atcgacgcag ccgatcaggc atcgatgccg tgagcgagcc
       61 agtcgagcgc gacagccgcc gtccacgact ggtccagcga gccgagcggc tccctggtga
      121 acggctcggc atactccgcg aagtcgattg cgcgcacctg cgccaacccg gcacgggcaa
      181 gctggtcggc atcgttcggc gcgccagacc ggcgtagcga ccaggcgaag agccagttca
      241 tcactggcca gaccggccct cgccagtagg tgcgcggcga aaactccggc tccagtggac
      301 tggtgctcgg tgggagtggc catcgaaggt ttggatgtcc gaccatcgcc tccgagcgcc
      361 acacctccag cagcgcatca cgccgctctg acgtgaggcc accggcaatc acgggcgcga
      421 agctcgccag cgtgcgcacc gtgacatccc gcccggcgac gaggtcgcgg tcgcagcaca
      481 tcactcgatc gtcgttccat tgctctgcga ggccgagccg accccgctcg atccagctct
      541 cgatgatctc cacctgctcg aggctcgcgt cggcgatgct cgccagccgc agcagcgcct
      601 cgttggcggc caccaggatc gcgctcatga agacgtcctt catacggaac ggaagcgtct
      661 ggtaggcggc gtcgacccgg tactcggcct cgaccagccg atcgacgatc cagaggtagc
      721 ggtcgtagtc agccttgctt ggacgctggg agggatcctc gacatgcttg aggtcggccc
      781 gctcgtatcg aacgaggcgc tccgggtcga cggtgatcgc ctccatcgca gcggtccagc
      841 ggggcgagtt gtccatcccg ctttcccacg gatgaacgat ggtaacgagc ccgctactct
      901 ccgaatcgcg cgccgagagc aggtagcggt gccagtcgaa gagtgggccg aagaattccc
      961 gcgctctgac tgcccgctgc tcctgcagca gcgcatcgac ggcccgcagg atgccgatgg
     1021 cgtgcacggg cggctgcacc agtccgctcg tgtccactcc ttccggggca tccggcgcga
     1081 aatcggcaca ggcccagcgc ttggggccag ggaagtactc tcccggagcg acatccggat
     1141 tgaagacgat gtgtgggatc atgccgttgc gccattggcc acgtatcagc gagcgcatct
     1201 cctccagcgc ccggcttgga tcgaaccggg cgagaccgat cgcgatgaag gcgctgtccc
     1261 agctccactg gtgagggtag agtctcggtg cagccttggt ccacccggcg agacggttcc
     1321 tgtcgaggac cgtgatcgcc tcgctccaga ggtcggtgtc acgcatggcg ttctgcagtc
     1381 gttcggcgtg catggcacac tagcggaacg gatgctgatg ctgttctagc acggcgcctt
     1441 ccggcctcca acccgtcctt tcatgcagga aaagcccatg gccgcatggc cgcacatccg
     1501 aaccgatgag cagacgcggg cggtcaccgc ctggtggatg cgcctcggca cgcagcccga
     1561 tggcgagatt cgggacgcgc tctacgatct tgttccgctc acgcgcgacg acctcgcgct
     1621 gatcgacacg acggcattcc agcgactcga ccgcgtccag cagctcggct tcgtctcgca
     1681 catctggcca ggagccaagc acacccgctt cgagcactcg ctgggggtga tgcacctcgc
     1741 ccgtcgatcg gcggtgcgcc tctcgcagcg ggcgaacggt ggctgggtca gactcgacga
     1801 catccgaacc gtcgccgccg cggcgctgct gcacgacatc ggccactacc ccttctcgca
     1861 tgcgatcgag gaactcggct cgccggtgct gccccatgag gcggtcggcc gccggctcat
     1921 cgaggagggg gagctcgccg acgtgctgcg cgatgcctgc aggctcgatc cggcacgggt
     1981 ggcgtcgctc attcatggcg agcgttccca gttgcccccg cgcgatcgca tcctcttcca
     2041 gatcctctcc ggcacgctcg atgtcgacaa gctcgattac ctgccgcgtg atgctcgtgc
     2101 ctgccacgtg ccctacggtg gggtcgacac cgatcgactg atcggcgcgc tcgacttggc
     2161 catgatcgag gcggtcgatc aggaggggga agatgcgcga gtggtggtga cgccgaaagg
     2221 gatcagcgcg ctgcactcgc tgatcaacgc tcgccaggag atgttcgaca acgtctactg
     2281 gcaccacacc aatcgcacct gcatggtcat gctcctgcgg gctgtacagg acgcactcgg
     2341 tcacgagcgc atcgccccgg aggagctctg gcgactcgac gatgcctcgc tgctcaaccg
     2401 cctgcaggcg cccgagatgc cgaacaccac ccgccagctg gcgactgcgc tcgcgacccg
     2461 ggacttccac aagcgggcgc tggaggtgag caaccgggcg atcgagctct acggccgcct
     2521 cggcaacctg tggttcgacc cgggggcgcg gcgtgaggtc gagtgccagc tggcggcatc
     2581 gctttccgag cggttggagc gggaggtcag cgaggcggca atcctgatcg acatcccgaa
     2641 acccgagaag tggaagagcg acgtctgggt cgtctatgag cgaccgcccg tcggcatgcg
     2701 caagcagatg cactggaaag aggtcgtcgg tgtcgacgac gccgatttca agcgttacga
     2761 ggagcaccgg cggctgatcc gtatcgtgac cagaccagag ttgcgggagg tcgtcgccgc
     2821 ggagtgggaa tcgctgatct atccgctgct gggaggcgtg ttctagaagg aggcagtggg
     2881 cgtgatggac ggaagagatg atgtggactc tcatggcgtg tcgacggtgg agatcgtgat
     2941 gctggccggc gtgctggcgg tgctggccct gctcgctctg attgtcatcg ccgcgatgta
     3001 gggccgggat gcttggtgac gatgtcggtg ggcctatcga caggtagcgc cgggtcctgt
     3061 gcccggcgcg tcgtcgattc cgcctatggt cgatctctaa acgccaatgc cgcccggtcg
     3121 gcccgcccct ctgtgggcgg gcatcacaat gctggctatc ac
//
LOCUS       DASYWL010000104        14197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_417831, whole genome shotgun sequence.
ACCESSION   DASYWL010000104 DASYWL010000000
VERSION     DASYWL010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 14197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14197
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417831"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="VGR22_03900"
     CDS             <1..1124
                     /locus_tag="VGR22_03900"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2127743.1"
                     /translation="LAGSAPSIGWFFAGRALQGFGAGGLFASVYAVIALAYHDRLRPA
                     MMAATSSAWIVPSLVGPAIAGFIAEQYTWRLVFYLLIPVLLVVAPLTLPAYHRMHQAR
                     PPMQVGAGRKRRLAMAAALALSTGIFLGGLELNPVALAALVTVAGLGGLVFALRHLMP
                     EGILTARPMLPAAIFIRSLCFGAFIVSETYMVFALKEFGGVSATTAGLVLTAGSLSWT
                     SGSWIQARWENASGVEGRPLRALSGLGCLLAGIGVIYGTMALTQDIWIGVAMPGWIIA
                     GLGMGLAYPTTSLIALGHAGEGSEGHVSSSILLGDLFISSTSVGLGGVLLAFGLDSGW
                     DPPAATTLAMSLGYALLLINLWATLRMLRAAPAMRRATA"
     gene            2270..3364
                     /locus_tag="VGR22_03905"
     CDS             2270..3364
                     /locus_tag="VGR22_03905"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEV2127744.1"
                     /translation="MLEGEPTVEAVAVADPEESAREAGLRYILDDRPGITRRRAGKGF
                     SYRDPNGERMTDTKVIERIKSLAIPPAWTEVWICPSPKGHLQATGRDAKGRKQYRYHP
                     RWREVRDENKYEHVLAFGKALPSIRERVDADLRKHGLPKEKVLALVVRLLETTLIRVG
                     NDQYAKENKSFGLTTMRDRHADIHGSTITFEFSGKGGIEHEVDLRDRRLARIVKQCQD
                     IPGQHLFQYVDDEGNRQPIDSDDVNAYLKEITGADYTAKDFRTWAGTKLAAEALAEFE
                     EFDSETAAKKNVVDAIERVAGRLGNTTTICRKCYVHPAIIDAYMDGDTVESIRQRAGQ
                     ELAEHLADLDPEEAAVLMLLHERLEENGSR"
     gene            3683..4738
                     /locus_tag="VGR22_03910"
     CDS             3683..4738
                     /locus_tag="VGR22_03910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV2127745.1"
                     /translation="MELGLVSFADATTNPATGQTISQGERLRNLIEEIELADQVGLEV
                     FGVGEHHRRDYAVSSPSTVLAYAAARTANIRLSSAVTVLSSEEPVRVFEQFATIDQLS
                     RGRAEIMVGRGSFIESFPLFGYDLNDYNELFAEKLDLLLKIRDNERVTWSGRHRAPLQ
                     DQAIYPRPCQDKLPIGVAVGGTPQSAVRAGMLALPMTLAIIGGQPERFVPFAEIYREA
                     GTQAGHDRASLHLAINTHGHVADSMEQAIEDTFAAYKGMMDQIGRERGWPPMTREQFE
                     AGTGLHGHLMMGSPEQVIEKILHQYELFHHQRYLIHFGIGAVEHEKVMHAIELFGAKV
                     APAIREEVAHREASVTP"
     gene            complement(5096..6934)
                     /locus_tag="VGR22_03915"
     CDS             complement(5096..6934)
                     /locus_tag="VGR22_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2127746.1"
                     /translation="MGFIMHGLDAEDYDRKYSDSELLRRILGYFRPAAAAMVLVGAMI
                     TLHSVMQMVQPVAISWGIDRIIQDAAGEILWLLAGIILTAGVLAWAFNFVRQSVTARV
                     VGDVVLALRTDAFDAAMERDLSFFDRNPSGKVVSRVTSDSDEFANVVTLTMNLLSQVL
                     LVFIVIGILYSRNAFLATVALAMTPVAIAIALVFRRVARETTLRAQRSIARVNAAIQE
                     AMNGIAIAKNFRQERALYNEFHPINEQSFQVQFRQGLVFAAIFPVLSAVAGLGTVLLV
                     YLGGRQAIDGDISAGDWFLFFQSVLLFWFPVTSIASFWSQFQQGLSASERIFALVDAD
                     REIVQTASEDPGRIRGEIEFRKLGFRYDQTQKVLHEFDLTIPAGQTLAIVGHTGAGKS
                     TLGKLITRFYEFHEGELLIDERDIRSFDLPEYRKQLGIVPQSPFLFSGTVADNIRYPK
                     PDATDEEVEAAARLVGNGDWVETLPNGLQTDVGEYGRSLSMGQRQLVALARVLIQDPA
                     IVILDEATASVDPLTEAQIQEGLDVVLADRTSIVIAHRLSTIEHADRIIVLDHGEIAE
                     EGTHRSLMAKGGRYCDVYNTYFRHQSPNYVPGEGFVGGNGVQSAAE"
     gene            complement(6937..8790)
                     /locus_tag="VGR22_03920"
     CDS             complement(6937..8790)
                     /locus_tag="VGR22_03920"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2127747.1"
                     /translation="MSDVQRPAAAIPPERTSAEFTTALPGRYDHRSALRWLLSHLSVT
                     KGWFAVFLAASVLAAAFNGFIPILTGQAFDEVLGTPDDPMAVLVRISLLLLGVVVLRG
                     VFDLLARIAIELVAKRVDRNTRQELFVSLLGKSQTFHNRQQVGDLMARATNDVRQINL
                     GFSPGIDLIFDGLTVLVAPFFFIAFIDPQLLLSPVAFLIAFVLALRLYMRQLTPVSDR
                     MRAQFGVMNAGLNESVRGVEVIKATGQERQERGRFRTQARLYRDYQVARGRVEARYLP
                     TLLLAVAVTGALFHGLWMVDRGRISIGDLIAYLGLMQQLSFPTFISIFSFAMVQMGLS
                     AADRILSVIREETELDQNEGGYTGPMRGEITFDDVTFRYETDESPAVENISFTARPGE
                     TIAIVGETGSGKSTLTKLVNRTFDVSSGHVLIDGVDVREWNLDALRSNISTIEQDVTL
                     FSRTIAENIGFGLGDQLDREAIVQAAKDAQAHEFIVEMEKGYDTVIGERGITLSGGQR
                     QRLAIARALVTQPRILILDDSTSAIDSATEDRIQTAIRRVLEGRTTLLITHRLSQIRQ
                     ADRVLLIRNGRIVDSGPHDELLATSQLYRRIFLHYDEVMQPESAPAGVEGA"
     gene            complement(8977..9753)
                     /locus_tag="VGR22_03925"
     CDS             complement(8977..9753)
                     /locus_tag="VGR22_03925"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV2127748.1"
                     /translation="MTEKRRVLITGARGRIGRCLTEGLKDRYILRLHNRTQTSEPEGF
                     DHATADLSEYDDVRPMLDGVDTVLHMAANPSVQAPWESIVSDNIVSTRNVLEAAKDAG
                     ARRVIFASSNHAMGMYDRDGEWPIYTSMPVRPDSHYGASKVIGEVFGRYYYDAFGLEF
                     ISLRIGWFTDQLATRKDHSILRAMWLGPRDAVNVVFGAIETTVPFGIYYAVSDNPDRR
                     WDITNTMVDLGYRPVESWETELGMEEGHQPGEPPRREEWL"
     gene            complement(9857..10801)
                     /locus_tag="VGR22_03930"
     CDS             complement(9857..10801)
                     /locus_tag="VGR22_03930"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2127749.1"
                     /translation="METKKLASAGKSSATSAGFRTGRGEASMLIEILGSGGADPAPRP
                     GCHCPTCEVARERGIPYARTGPSTFVHGANILFDTPEETRGQLNRAGIDRVTACFYSH
                     WHPDHVMGRRVFELLNWDVYGWPPRLHVTDVYLPEQVAVDFEAHLGGAEHFSYLEQIG
                     VVRVHEVRDGTSVTVEGVTVTPIRLAESYVYAFFVEHAGTRVLIAPDELVGWSPPAWL
                     EGLDLAVLPMGICEHDPFTGERKVHEDHPILQLEMTFPETIEVVRGLRPTRAVLTHIE
                     EADDPGPDILNQLAAMHQEGSLPITFAHDTMRIEVGPA"
     gene            complement(10949..11431)
                     /locus_tag="VGR22_03935"
     CDS             complement(10949..11431)
                     /locus_tag="VGR22_03935"
                     /inference="COORDINATES: protein motif:HMM:NF022623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HEV2127750.1"
                     /translation="MVSRGSGTTSAPISPWGSNNSGWWGEGEIKFYMDADDEWPTICS
                     TGTEDYFGGAWNFEEVPDTYTPYTTPYLGLPQVIVPTGMRNSQYRFGMYRWHVPDPIR
                     FEERLRVTIQALAWRRPLEGKPRYLPLQDDIASTAFWYQAEPHAPFPVLGDFDTLEVI
                     "
     gene            complement(11319..12044)
                     /locus_tag="VGR22_03940"
     CDS             complement(11319..12044)
                     /locus_tag="VGR22_03940"
                     /inference="COORDINATES: protein motif:HMM:NF022623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HEV2127751.1"
                     /translation="MTFDGLGTHIGNLARRSHARTRSISAENPTGALGQGGMVTEGTG
                     SVAARDLGQGWKVSPSIDVAGSETVTLAEIEGPGAIQHIWLTVHPQHWRSLVLRMFWD
                     GEDAPSVEVPLGDFFCNGWCQRSNVASIPVAVNPAGGFNSYWQMPFRRSARATLENLT
                     PDQIKGFYYQITWTETGIPEDSAYLHAQWRRSNLLPYGEVHTLLDGVEGFGHYVGTYI
                     ALGQQQQRLVGRGRDQVLHGCRR"
     gene            12534..12656
                     /locus_tag="VGR22_03945"
     CDS             12534..12656
                     /locus_tag="VGR22_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127752.1"
                     /translation="MTENERRAQDVLTREDLILRGFTVTGLIAMFLLVLLVVSP"
     gene            12827..13678
                     /locus_tag="VGR22_03950"
     CDS             12827..13678
                     /locus_tag="VGR22_03950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HEV2127753.1"
                     /translation="MSDDTQLADDALARVHDHRVPDPKSFKIKKTEPDSIGGFGDDDK
                     PLAKAELKVLRQRLQDLQERLYAEHKQSLLIIFQATDTGGKDSTTRRVFRGVNPQGVR
                     VWSFKQPSAEELDHDFLWRYHDHTPIKGMIGIFNRSYYEDVLVVRVKELAPKEVWKHR
                     YDHINAFERLLADNGTRALKFFLDISKEEQRERLQERLDKPDKHWKFHSADLADREHW
                     GNYQHAYEAMIRRTSTEHAPWYVVPANRKWYRDLVVARTIVETLEEMDPQFPPPEEGL
                     DEIEIPD"
     gene            13697..>14197
                     /locus_tag="VGR22_03955"
     CDS             13697..>14197
                     /locus_tag="VGR22_03955"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEV2127754.1"
                     /translation="MQWLFDNWAGLGRTLLVGTLAYVGLVLMLRVSGKRTLSKMNAFD
                     FVVTVAMGSTLASTLLSQSVALVEGLLAMGLLISLQFVITWLSVRSDTVARIVKDEPT
                     LIYHHGQYLDRQLKRMRVTRSEVQAAVRQTGKLSMDAVDAVVLETDGTFSVLSASGDT
                     SAATIPV"
BASE COUNT         2621 a         4685 c         4416 g         2475 t
ORIGIN      
        1 tcctggccgg ctccgcgccg tcgatcggct ggttcttcgc cggccgcgcg ctccagggct
       61 tcggcgctgg cggactcttc gcctccgtct acgccgtgat cgctctcgcc taccacgacc
      121 gcctccgccc cgcgatgatg gcggccacct cgagcgcctg gatcgtgccg tcactcgtcg
      181 gcccggcgat cgccgggttc atcgccgagc agtacacctg gcggctcgtc ttctacctgc
      241 tcatcccggt gctcctcgtc gtcgcgccgc tgacccttcc cgcctaccac cgcatgcacc
      301 aggcgcgacc gccgatgcag gtcggcgcgg gacgaaaacg ccgcttggcc atggcggcgg
      361 cgctcgccct ctccaccggc atcttcctag gcgggctgga gctgaatccg gtcgcactcg
      421 ccgcgctggt gacggttgcc gggctgggtg gtctcgtctt cgcgctgcgc cacctgatgc
      481 ccgaggggat cctcaccgcg cgcccgatgc tcccggcggc gatcttcatc cgcagcctct
      541 gcttcggggc attcatcgtc tcggagacct acatggtctt cgccctcaag gagttcggcg
      601 gcgtcagcgc caccaccgcc ggtctcgtat tgacggcggg gtcgctgagc tggacctccg
      661 gctcgtggat tcaggcacgg tgggagaacg ccagtggcgt cgaggggcgt cccctgcgcg
      721 cgctttccgg tctcggttgc ctgctcgccg gcatcggcgt catctacggc acgatggcgc
      781 tcacgcagga catctggatc ggtgtggcga tgccaggctg gatcatcgcc gggctgggta
      841 tgggactcgc ctaccccaca acgtcgctga tcgcactcgg gcatgccggc gaggggtcgg
      901 aaggccacgt ctcctcgtcg atcctgctcg gcgacctttt catctcgtcg accagcgtcg
      961 ggctcggggg tgtgttactg gccttcgggc tcgactccgg ctgggatccc cccgcggcaa
     1021 ccacactggc gatgtcgctc ggctacgcgc tgctgctgat caatctctgg gcgacgcttc
     1081 ggatgctccg cgccgccccg gcgatgcgcc gagcgactgc ttgagtcact tgtcaaatga
     1141 tgtagctccc ctcctccaga aagttggcac tagctctcat gcttgcttcg atgagggtga
     1201 tcgcttctgc cttgaatgcc attcatgcct gtgcccgaag agagtgaagg cacttcgaga
     1261 actgatcaag cctgccacac tcgactgacg acatctctag ggcgattagg tgacgggggt
     1321 agtgatacgg acccgcagcg agcataagtc gtccaagacc tccgtctcag ccgagaactg
     1381 ccgtgggtgc ttgtcaatag gcgggttctg ctcaactaga aggaacagaa agttgaccgt
     1441 tatttatcag aaaaagccga cgaagaacta gtcctcgggg tcgaggactc caggaggcat
     1501 ccaatactcg ccaaacgggg gatcaggata gcgccagatt actccacgcc atcgaacata
     1561 ctcatcgtcc cagaagaatc cgttctcgga aggtccgctt ggacccgaga aatactcagg
     1621 cggaatggtg cgcacgtgct cgttagcggc ctctaggcgc ttcaggagcg cttcttcgtc
     1681 aagacttcca tgaaagtaat agcgaaccgg ggcgacagga tacgcctctg aggactcctc
     1741 cccaatggcg atctacaagg gcacataccc tcgacctgac tccttgcttc ggcgcccgct
     1801 ccgtatcaat cgcagaacct catatcggta tgcttcgccg gccgccacaa caagcggctc
     1861 gaaaaacttg aaaaggagcc cgaacgtacc cgtcgcgacg atcgtgaacg cgatctctga
     1921 aaacccagca gcaggtggct cataatcgag actggatgac tcaacgctgg tgtcatatag
     1981 cgatgcaagg ccttgattga ggtcgtcagt cagctccggc ccgacgaccg gcaggatgct
     2041 ttgagtatga attcggtacg cagatgtcat tgcctctcct cccgtcctat gcaagatggc
     2101 ggcatcttac cgtgaggcgc tgctcgaaac tcgacgtcgt accggcgatt ggaatgaacg
     2161 acggaagcga tcctgatcgg ctcagcgagg aacatccgtc ccacctgtct ggcatgtgtt
     2221 ctgcgtgaga gcagaccggt cccgactggg acccagaggg agacgacaca tgctcgaagg
     2281 agaaccgacg gtcgaagctg tcgcggttgc cgatccggaa gaatctgccc gcgaggccgg
     2341 gctacgctac atcctcgatg accgcccggg catcactcgt cgccgggccg gcaaggggtt
     2401 ctcctaccgt gaccccaacg gcgaacgcat gaccgacacg aaggtgatcg agcgcatcaa
     2461 gtcgctcgcc atcccgcccg cctggaccga agtctggatc tgcccgtcgc ccaagggaca
     2521 cctgcaggcc accggccgcg acgcgaaggg ccgcaagcag taccgctacc atcccaggtg
     2581 gcgcgaagtc cgcgacgaga acaagtatga gcacgtcctc gccttcggca aggcgctccc
     2641 gtcgattcgg gagcgtgtcg acgccgacct gcgcaagcac ggcttgccaa aggagaaggt
     2701 gctggcgctc gtcgtccggc tgctggagac aacgctgatc cgcgtcggca acgaccagta
     2761 cgcgaaggag aacaagtcgt ttgggctgac gaccatgcgc gaccgccacg ccgacatcca
     2821 cggctcaacg atcaccttcg agttcagcgg caagggcggc atcgagcacg aggttgacct
     2881 gcgcgaccgc cgcctcgccc gcatcgtcaa gcagtgccag gacatccccg gccagcacct
     2941 cttccagtac gtcgatgacg agggcaaccg ccagccgatt gactcggacg atgtcaacgc
     3001 ctatctcaag gagattactg gcgccgacta caccgccaag gatttccgca cttgggcagg
     3061 gaccaagctg gccgccgagg cgctcgccga gttcgaggag ttcgactccg agacagccgc
     3121 caagaagaac gtcgtggacg ccatcgagcg ggttgccgga aggctgggca acaccacgac
     3181 gatctgccgc aagtgttacg tgcacccggc gatcatcgac gcctacatgg acggcgatac
     3241 cgtcgaatca attcgccagc gggctgggca ggagctggcc gagcacctcg ccgacctcga
     3301 cccggaggag gcggccgtcc tgatgctgct ccacgagcgc ctggaagaga acggttccag
     3361 atagcgcacg caagtcttct atcatgccga gccgacgagg cgccttggcg tcgggacaag
     3421 tcgaagcatc cctgcgcgag gcgccggcac tgtaggtgtc ggcctctgtc gatcgttgtg
     3481 gtgcttgagg ccgggaagct gccctgcggg cagcccactt cgcgcgggga ctcctcgcta
     3541 cgctcggaat gaggtcgagg gggcggcaca gcgccagagt gtctggagag gtacgcacac
     3601 gacttaacct cagcaggcaa tccgaggtgt gacaattgcc ctgttgcgtc acgccgccgc
     3661 attcagcaag gactctgact tcatggaact cggactcgtc agcttcgccg acgccacgac
     3721 caatcccgcc accgggcaga ctatcagcca aggtgaacgc ctgcgcaacc tcatcgagga
     3781 gatcgagctg gccgatcagg tcgggctcga ggtcttcggt gtcggggagc atcaccgccg
     3841 cgactacgcc gtctcctcgc catcgaccgt gctcgcctac gccgccgcac ggaccgcgaa
     3901 catccgcctc tccagcgccg tgaccgtcct cagctccgag gagcccgtcc gcgtcttcga
     3961 gcagttcgcc accatcgatc aactctccag gggacgcgcc gagatcatgg tcgggcgcgg
     4021 ctcgttcatc gagtccttcc cgctcttcgg ctacgacctg aatgactaca acgaactctt
     4081 cgccgagaag ctcgacctcc tgctgaagat ccgcgacaac gaacgcgtta cctggtccgg
     4141 gcggcaccgg gcacccctgc aggaccaggc gatctatccg cgcccatgcc aggacaagct
     4201 gccgatcggg gttgccgtcg gcggcacgcc gcagtccgcg gtgcgggccg gcatgctggc
     4261 gctcccgatg acgctcgcga tcatcggcgg tcagccggag cggttcgtac cattcgccga
     4321 gatctaccgg gaggcaggca cgcaggccgg acacgaccgg gcatccctcc atctggcgat
     4381 taacacgcat gggcacgtcg ccgactcgat ggagcaggcg atcgaggata ccttcgccgc
     4441 ctacaagggg atgatggacc agatcggccg cgagcgaggg tggccaccga tgacccgcga
     4501 gcagttcgag gccggcaccg gcctgcacgg gcatttgatg atgggctcgc cggagcaggt
     4561 gatcgagaag atcctccacc agtacgagct gttccatcac cagcgctacc tgatccactt
     4621 cggcatcggc gccgtcgagc acgagaaggt catgcatgca atcgagctct tcggcgcgaa
     4681 ggtcgccccc gccatccgcg aggaggtcgc ccaccgcgaa gcatccgtca ccccgtagcc
     4741 ggcgcaaccg tgcagcgccg ggtcctgtgc ccggcgtttc gcccgaaggt ggccatgcca
     4801 ccgggaaacc cgtgtcacga tcagacacct tggtgtagac ccgggctgaa atgcccgggt
     4861 ttcgtccatt gaggcaggat tccgctgacc accaacaacc actgaaccgg cgttgcaccg
     4921 gtccaacgcc cagacggccg cccgccaaca gaatcgaatg atcaagacgc gggagccaga
     4981 gcgaagcggt gcgtgtggct cgactccacg gcgaccctgt cctatcccca cacaatgccg
     5041 ttcttaacgt caatccacgc atctcaccgc gtatctactc agtcacttcg atggttcatt
     5101 cggcggcgct ctgcacccca ttgccaccta cgaacccctc gccgggcaca tagttcggcg
     5161 attggtggcg aaagtaggtg ttgtagacgt cgcagtatcg cccgcccttc gccatcaggc
     5221 tccggtgcgt cccctcctcg gcgatctcac cgtggtcgag cacgatgatc cggtcggcgt
     5281 gctcgatcgt cgagagccgg tgtgcgatca cgatcgacgt ccggtccgcc agcaccacgt
     5341 cgagcccttc ctggatctgc gcctcggtga gcgggtcgac gcttgccgtc gcctcgtcga
     5401 ggatgacgat cgccgggtcc tggatcagca cccgcgcgag cgccaccagc tgccgctgcc
     5461 ccattgagag cgagcgcccg tactcgccga cgtccgtctg cagaccgttc ggcagcgtct
     5521 cgacccagtc gccgttcccg accaaccgag ccgctgcctc gacctcctcg tcggtggcgt
     5581 ccggcttcgg gtagcggata ttgtctgcga cggtgcccga aaagaggaag ggcgactgcg
     5641 gcacaatgcc gagctgcttg cggtactccg ggagatcgaa cgagcggatg tccctctcat
     5701 cgatgagcag ctcaccctcg tggaactcgt agaaccgcgt gatcagcttg ccgagggtcg
     5761 acttccccgc gccggtgtgc ccgacgatcg ccagcgtctg cccggcgggg atggtcaggt
     5821 cgaactcgtg cagcaccttc tgcgtctggt cgtagcgaaa cccgagctta cggaactcga
     5881 tctcgccccg gatgcgcccg ggatcctcgc tcgccgtctg gacgatctcc cggtcggcgt
     5941 cgaccagcgc gaagatgcgc tccgaagcgc tcaatccctg ctggaactgg gaccagaacg
     6001 atgcaatgct cgtgaccggg aaccagaaga gcagcacgct ctggaagaag aggaaccagt
     6061 cgccggccga gatgtccccg tcgatcgcct gccggccacc gaggtagacc aacagcaccg
     6121 tgccgagtcc ggcgacggcg ctgagcaccg gaaagatcgc ggcgaagacg agtccctggc
     6181 ggaactgcac ctggaacgac tgctcgttga tcgggtggaa ctcgttgtac agcgcgcgct
     6241 cctgccggaa gttcttggcg atcgcgatgc cgttcatcgc ctcctggatc gccgcgttga
     6301 cgcgcgcgat cgagcgttgt gcccgcaagg tcgtctcccg cgcaaccctg cggaacacga
     6361 gcgcgatcgc gatcgccact ggcgtcatcg ccagcgccac cgttgccagg aacgcgttgc
     6421 ggctgtagag gattccgatc acgatgaaga ccagcagcac ctggctgagc aggttcatcg
     6481 tcagggtgac gacgttggcg aattcgtcgg agtcgctggt gacacgggaa acgaccttgc
     6541 cggacgggtt cctgtcgaag aacgagagat cgcgttccat cgcggcgtcg aacgcgtcgg
     6601 tacggagcgc gagcaccaca tcgccgacca cccgtgccgt caccgactgc cggacgaaat
     6661 tgaaggccca cgcgagcacg ccggcggtga ggatgatgcc ggcgagcagc cagaggatct
     6721 ctccggcggc gtcctggata atccgatcga tgccccagct gatcgccacc ggctgcacca
     6781 tctgcatcac ggagtgcagc gtgatcatcg ccccgacgag caccattgcc gccgctgccg
     6841 gccggaagta acccaagatg cggcggagca actcgctatc actgtacttc cggtcgtagt
     6901 cctcggcgtc gaggccgtgc atgatgaaac ccacacctat gctccttcca cacccgctgg
     6961 agcggattcc ggctgcatca cttcgtcgta gtgcaggaag atgcgccggt acagctgcga
     7021 agtcgccagc agttcatcat gagggccgct gtcgacgatc ctgccgtttc ggatgagcag
     7081 cacgcggtcc gcctgccgga tctgcgagag ccgatgggtg atcagcagcg tggtgcggcc
     7141 ctccagaacg cgccggatcg ccgtctggat ccggtcctcg gttgcgctgt cgatcgcgct
     7201 cgtggagtcg tcgagaatga ggatgcgcgg ctgcgtgacg agcgcgcgcg cgatcgcgag
     7261 gcgctggcgc tgcccgccgg acagcgtgat ccctcgctcg ccgatcaccg tgtcgtagcc
     7321 cttctccatc tcgacgatga actcgtgcgc ctgcgcatcc ttcgctgcct gcacgatcgc
     7381 ctcgcggtcg agctggtccc cgaggccgaa gccgatgttc tcggcgatcg tgcgcgagaa
     7441 gagggtgacg tcctgctcga tcgtcgagat atttgagcgc agcgcgtcga gattccattc
     7501 acgcacgtcc acgccgtcga tcaggacgtg tcctgagctg acgtcgaagg tgcggttgac
     7561 gagcttcgtc agcgtgctct tgccggagcc ggtctcgccg acgatcgcga tcgtctcgcc
     7621 tggccgtgcg gtgaagctga tgttttcgac ggcgggcgac tcgtccgttt cgtagcggaa
     7681 ggtcacatca tcgaaggtga tctcgcctcg cataggcccg gtgtagccgc cttcgttctg
     7741 gtcaagctcg gtctcctcgc gaatcacact aaggatgcgg tcggcggcgg agagccccat
     7801 ttgcaccatg gcgaaggaga agatggagat gaaggtcggg aagctcagct gctgcatcag
     7861 tccgaggtag gcgatcagat cgccaatgct gatccttccc cggtcaacca tccagagccc
     7921 atggaagagc gcgccggtga ccgccacggc cagcagcagg gtcggcaggt agcgtgcctc
     7981 gacccggccc cgtgccacct gatagtcgcg atagagccgc gcctgggtgc ggaagcgacc
     8041 acgctcctgc cgctcctggc cggtcgcctt gatcacctcg acgccacgga cggactcgtt
     8101 cagcccggcg ttcattaccc cgaactgggc tcgcatcctg tcggacaccg gcgtgagctg
     8161 gcgcatgtag agccgcagcg ccagcacgaa ggcgatcagg aaggcgaccg gcgagagcag
     8221 cagctgcggg tcgatgaagg cgatgaagaa gaacggcgcc accagcaccg tcaggccatc
     8281 gaagatgaga tcgatgccgg gcgagaagcc gagattgatc tggcggacat cgttggtggc
     8341 gcgcgccatc aggtcgccga cctgctgccg attgtggaag gtttggctct tgccaagcag
     8401 ggaaacgaac aactcctgcc gggtattgcg gtcgacgcgc ttggcgacca gctcgatcgc
     8461 gatacgagcc agcagatcga agacgccgcg cagcaccacc acgccgagca gcaacaggga
     8521 gatgcgtacc agcaccgcca tcggatcatc gggcgtgccg agcacctcgt cgaatgcctg
     8581 tccggtcagg atggggatga acccgttgaa ggctgcggcg agcaccgacg cggcgagaaa
     8641 tacggcgaac caccccttcg tcacggagag atgcgagagc agccagcgga gggccgagcg
     8701 gtgatcgtac cggcccggca gcgcagtggt gaactcggcg ctggtgcgct cagggggtat
     8761 cgcggcagcg ggccgctgga cgtcggacac gatcgtgctc cggagcgtga gatgacgtga
     8821 ggacccggga ggcgatcaga ctcgcctcaa cagcgacgca cagtatccca cgaatggccg
     8881 aaagaaacct gctggccaac tgcgctcggt accaccaacc aaccacccgt caccctgagc
     8941 gcagcgacgg gtccccgtgc gaagcactcg gcagcgttac agccactcct cgcgacgggg
     9001 cggctcgccg ggctggtgac cttcctccat ccccagctcc gtctcccagc tctccacggg
     9061 gcggtaaccg aggtcgacca tcgtgttggt gatgtcccag cgccgatccg ggttatcgga
     9121 gacggcatag tagatgccga acggcacagt cgtctcgatc gccccgaaca cgacgttgac
     9181 cgcatcgcgc ggcccgagcc acatcgcccg caggatcgag tggtccttgc gagtggcgag
     9241 ctggtcggta aaccagccga ttcgcagcga gatgaactcc agcccgaagg catcatagta
     9301 gtagcgaccg aagacctcgc cgatcacctt cgaggcgccg tagtggctgt ccggccgcac
     9361 cggcatcgaa gtgtagatcg gccattcgcc atcacggtca tacatgccca ttgcatggtt
     9421 ggacgaggcg aagatgacgc gacgcgcccc ggcatccttc gccgcttcga ggacgttgcg
     9481 cgtggagacg atgttgtcgc tcacgatcga ctcccacggt gcctgcacgc tcgggttggc
     9541 ggccatgtgg agcaccgtgt cgaccccgtc gagcatcgga cgcacgtcat cgtactcgga
     9601 gagatcggcg gtggcgtggt cgaagccctc gggctcggac gtctgggtgc gattgtgcaa
     9661 ccgaaggatg tagcgatcct tcagtccctc cgtgaggcac ctgccgatcc gtcccctggc
     9721 cccggtgatc agcacccgtc gcttctcggt catctcccgc tccccgtcgt cttccggtag
     9781 gactgttcgt cccgggcagc atagccgatc cccagcctgc ccacggcgct gcttgcatga
     9841 tgttcggaag ggcctctcag gcgggtccga cctcgatccg catcgtgtca tgcgcgaacg
     9901 tgatgggcag gctcccttcc tgatgcatcg ccgcgagctg attgaggata tcagggccag
     9961 gatcgtcggc ctcctcaatg tgggtcagca ctgctcgcgt tggccgcagc ccacgcacga
    10021 cttcgatggt ttccgggaac gtcatctcca gttgcaggat gggatggtcc tcgtgcacct
    10081 tccgctcgcc ggtgaagggg tcatgctcgc agatacccat cggcagcact gcgagatcga
    10141 gcccttccag ccacgcaggc ggagaccacc cgaccagctc atccggcgcg atcagcacgc
    10201 gggtacccgc gtgctcgacg aagaatgcat aaacgtagga ctccgcgagc cggatcggtg
    10261 tcaccgtcac cccctcgacg gtaacgcttg tcccatcgcg gacttcgtgg acgcgcacca
    10321 ccccgatctg ttccaggtac gagaagtgct ccgcgccgcc gagatgtgcc tcgaaatcca
    10381 ccgcgacctg ctccggcagg tagacgtcgg tcacgtgtaa cctgggaggc cagccgtaga
    10441 cgtcccagtt cagcagttcg aagacccggc ggcccatgac atgatcggga tgccagtgcg
    10501 agtagaagca tgcagtcacg cgatcgattc ccgcccggtt cagctggcca cgagtctctt
    10561 ccggggtgtc aaacaggatg ttcgcgccgt gaacgaaggt ggacggcccg gtgcgcgcgt
    10621 acggaatgcc cctctcccgt gcgacctcgc acgttggaca gtgacagccc ggacgtggcg
    10681 ccgggtccgc accgccactc cccagaattt ctatgagcat ggatgcctct cctctaccgg
    10741 tccggaaccc agcacttgta gcggaggact tgcccgcgct cgccagcttc ttggtttcca
    10801 tcgcacgtgc ggggcaatgt cctccttggc cgagacccga tgcagtcgaa ggacgccatt
    10861 ggctgcgcgg agtcctgttt tctcatcacc ctgacagcac agccaagtca tccactacgg
    10921 aacttgggcg aacagagagc ccgtcgcttt agatcacctc cagcgtgtcg aaatctccga
    10981 gaacggggaa cggcgcgtga ggctccgctt ggtaccagaa cgcggtcgag gcgatatcgt
    11041 cctggagcgg caggtagcgt ggcttcccct ccagcgggcg ccgccaggcg agcgcctgga
    11101 tcgtcacccg cagccgctcc tcgaaccgga tcggatccgg aacatgccac cggtacatcc
    11161 cgaagcggta ctgcgagttg cgcatgccgg tcggcacgat cacctggggc agtccgagat
    11221 acggcgtggt gtacggcgtg taggtatccg gcacctcctc gaagttccac gcaccgccga
    11281 agtaatcttc cgtgccggtg ctgcagatcg tcggccactc atcgtcggca tccatgtaga
    11341 acttgatctc gccctcgccc caccagccgc tgttgttgct gccccagggc gatataggtg
    11401 ccgacgtagt gcccgaaccc ctcgacacca tcgagcagcg tgtgcacctc accatagggg
    11461 aggaggttgg agcgccgcca ctgcgcatgc aggtaggcac tatcctcggg gatccccgtc
    11521 tcggtccacg tgatctggta gtagaagccc ttgatctggt ccggcgtcag gttctccagc
    11581 gttgcgcgtg ccgaccggcg gaagggcatc tgccagtagc tgttgaagcc tcccgccgga
    11641 ttgaccgcaa ccgggatcga ggcgacattg cttcgctggc accatccgtt gcagaagaag
    11701 tccccgagag gcacctcgac gctcggtgcg tcctcgccgt cccagaacat gcgcagaacc
    11761 agcgagcgcc agtgctgcgg gtgtaccgtg agccagatgt gctggatcgc gcctggaccc
    11821 tcgatctcgg ccagcgtcac cgtctcgctt ccagcgacat cgatcgatgg cgacaccttc
    11881 cagccctgcc cgaggtcgcg cgccgcgaca gagccggtcc cctcggtcac catgccaccc
    11941 tggcccagcg cgccggttgg gttctccgcg ctgatggagc gcgtccgagc gtgcgagcgc
    12001 cgcgcgaggt tgccgatgtg cgtcccgagt ccgtcgaagg tcatggtgtc ctcctctgtg
    12061 ctgttcttcc gggccgatgg ctccaagatt gggacgcggg acgggatcgc gcaagagcgt
    12121 tgacgtcttc caatagctgg cgctcatgct catggaacgc ctcgtgcgag ggagatcgtc
    12181 gtatcccgta ccgggtgacc aatccgtgtc gcctgcaccg tcgtcccgtg aagagacttg
    12241 cgttgccagt ttgtcacgat ggcggcacac gtctggattg atgccgccgg atagccggta
    12301 cactgactgg atcggagtga cgctcgattg acacggcgac accagaccgc tacactcgat
    12361 gaggaagcgt accgatgtac gtacaccttt gagccgaggt ctggaggggc cgaggttctg
    12421 gttggtaacc cgcagtccga tcgaccgaca agggtttcag tggccgtcct cgcagggtca
    12481 cgccaagcgt gctgcgatgc gaacggccag aagtgggggg gaaacacaat ccaatgaccg
    12541 agaatgaacg ccgtgcgcaa gacgtcctta cccgcgagga tctgattctc cgcgggttca
    12601 ccgtcactgg cctcatcgcc atgttcctgc tcgtgctgct ggtggtcagc ccgtaagcga
    12661 cacacgcgaa ttcggcagtt cttcactaat cccatggcat ttatatgtat attcgagcgg
    12721 cgtccctcga gggacgccac tcttcgcggc tgcatgctcc tccgttggca ccgtccttgc
    12781 caagtctctc gctgacgaac catcgtgggg aacagcggag agcgagatga gtgacgacac
    12841 gcagctggcc gacgatgccc ttgcacgcgt gcatgaccac cgcgtcccgg atccgaagtc
    12901 attcaagatc aaaaagaccg agcccgactc catcggcggc ttcggcgacg acgacaagcc
    12961 gctcgctaag gcagagctga aggtcctccg ccaacgcctt caggatctgc aggagcggct
    13021 ctacgccgag cacaagcaga gtctgctgat catcttccag gcaactgaca ccggcggcaa
    13081 ggacagcacc accaggcgtg tcttccgcgg cgtcaaccct cagggcgtac gcgtctggtc
    13141 gttcaaacaa ccgagcgcag aggagctcga ccacgacttc ctctggcgct atcacgacca
    13201 cacgcccatc aagggcatga tcgggatttt caaccgctcg tactatgagg acgtgctggt
    13261 cgtgcgcgtc aaggagctgg cgcccaagga ggtctggaag caccgctacg accacatcaa
    13321 tgcgttcgag cgattgctcg ccgacaacgg cacacgcgcc ctcaagttct tcctcgacat
    13381 ctcgaaggag gaacagcgtg agcggctgca ggagcgcctc gacaaacccg acaagcactg
    13441 gaagttccac tcagccgatc tggccgaccg cgagcactgg ggcaactacc agcacgccta
    13501 cgaggcgatg atcaggcgga cgagcaccga gcacgccccg tggtatgtgg tgccggcgaa
    13561 ccgcaagtgg tatcgcgacc tcgtcgtcgc ccgcacgatc gtcgagacgc tcgaggaaat
    13621 ggacccgcag ttcccgcctc ccgaggaggg gctagacgag atcgagatac cggattagcg
    13681 accgaaggga acgtggatgc agtggttgtt cgacaactgg gccggcttgg gccgcacgct
    13741 ccttgtcggc acgctcgcct atgtcggcct cgtgctgatg ctgcgcgtgt cgggcaagcg
    13801 cacactctcg aagatgaacg cgttcgattt cgtggtcacg gtcgccatgg gatcgacgct
    13861 ggcctcaacg ctgctgagcc aatcggtcgc cctcgtcgaa gggctgctcg ccatgggcct
    13921 gctcatcagc ctgcagttcg tgatcacctg gctctcggtc cgatcggaca ccgtcgcgcg
    13981 aatcgtcaag gacgagccca ccctgatcta ccaccatgga cagtaccttg accgacaact
    14041 caagcgtatg cgggtaaccc gttcggaggt acaggcagcg gtccgacaga ccggcaagct
    14101 ctcgatggat gcggtcgacg cggtcgtgct ggagacggat ggcaccttca gcgtcctcag
    14161 cgcgtccggg gacacttcag cagcgacgat accggtc
//
LOCUS       DASYWL010000105         3655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_511452, whole genome shotgun sequence.
ACCESSION   DASYWL010000105 DASYWL010000000
VERSION     DASYWL010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511452"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2225)
                     /locus_tag="VGR22_03960"
     CDS             complement(<1..2225)
                     /locus_tag="VGR22_03960"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEV2127755.1"
                     /translation="MADDRAALRARVSDEQVRERVDSLLAEMTVEEKAGQLTQYFYFG
                     FLRDMPAGEGDDEFNQPDLVEAALARGEAGSLLFVREPAEFNRLQKLAIDGNGHDIPL
                     IFGFDVIHGFRTILPVPIAMASSWDPEVVERGQVVAAREARAVGIHWTFAPTVGIARD
                     PRWGRIVEGAGEDAFLGAAMAVAQVRGFQGEGIGAPERVVAGPKHFAGYGAALGGRDY
                     DEANISDYELWNTYFPPFKAALDAGAGNVMTAYMSLNGVPATGNPWLFDEVLRQTWDF
                     EGWVVSDANAVRNLKTHGYAKDLAEAGVRGLTAGCDMEMAVADPAYAHLPEALADGRI
                     DESTFDASVRRVLEAKVRLGLFEEPFVDEDRARQVVRDPAHREVAREAAERSAVLLKN
                     GGDLLPLSADTRGSLAVIGPLADSRRDTLGPWCFEFDLEETVTVLEGIREKVGDAVTV
                     EYAPGIRPAQRTFPSMFDMWPGNAPEDPENFDDEAEFRRAVDLARESDIAIVVVGEWQ
                     NMIGEAASRSSLELPGRQLELLQAVAETGTPVVLLVMNGRPLDLRWAVEHVPAILDIW
                     YPGTRGGEAVANLLFGDAVPGGKLPFTWPRAVGQVPMIYNHTASHEPQKQGTRYWDEA
                     STPLFPFGHGLSYGRFEYRNPTLDRDRIAAGETLTVSVEVVNIGACTGDEVVQLYIHQ
                     RHGRAARPVRELKGFRRVTLEPEASQRIEFTLGPDELRYWHALERDWVIDAATFDVWV
                     GG"
     gene            2353..2598
                     /locus_tag="VGR22_03965"
     CDS             2353..2598
                     /locus_tag="VGR22_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127756.1"
                     /translation="MRYGTTTKRSPREVIDQACAFFGPEGELGLPEASTRNNTITFCS
                     EAGFLSIACDPTPDGTEVTVLSREYDTWAEQFIRKIG"
     gene            2829..3581
                     /locus_tag="VGR22_03970"
     CDS             2829..3581
                     /locus_tag="VGR22_03970"
                     /inference="COORDINATES: protein motif:HMM:NF015551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HEV2127757.1"
                     /translation="MSDERLSREEHASSDTSTPAKGLWLTWIGAALPMIILPVVIVTI
                     PVAVGKSMRLTSDKLTSWIVALYAIPSALSLAFALLYRQPLVLTGNVFFMIFIARLGG
                     DIAFAELVGASLVAGAVVLAVVAMGVSDHLASLVPTPVVLGLLAGAVLPFVVDLFSLL
                     GEEPVAVGGMGLAYLLGQRFLSARLPPILPALIAASLLAPVTRNAGSLPTDLPLVTAM
                     VFTLRFTLDGLLTVMPVLVILILLQSNLPSVI"
BASE COUNT          613 a         1300 c         1100 g          642 t
ORIGIN      
        1 cccccgaccc agacatcgaa cgtcgctgcg tcgattaccc agtcacgctc caacgcatgc
       61 cagtagcgca actcatccgg gccgagcgtg aactcgatcc gctgcgaggc ctctggctcc
      121 agcgtcaccc gccggaagcc cttcagctcg cgcaccggcc gcgccgcgcg gccgtgccgc
      181 tgatggatgt agagttgcac cacctcgtcg cccgtgcacg ctccgatgtt gaccacctcc
      241 accgacacag tcagcgtctc gcccgccgcg atgcggtccc gatcgagcgt cgggttgcgg
      301 tactcgaacc tgccgtagct caggccgtgg ccgaacggga agagcggcgt gcttgcctcg
      361 tcccaatagc gggtgccctg cttctgaggc tcatgtgacg cagtgtggtt gtagatcatc
      421 ggcacctgcc cgaccgcccg cggccaggta aacggcagct tgccgcccgg cacggcatcg
      481 ccgaagagca ggttggccac cgcctcgccg ccacgcgtgc cgggatacca gatatcgagg
      541 atcgccggga cgtgctcgac cgcccagcga aggtcgagcg gccggccgtt catcacgagc
      601 agcacgaccg gggtaccggt ttcggcgacc gcctgcagca actccagctg gcgaccgggg
      661 agctccagcg aggatcggga tgctgcctcg ccgatcatgt tctgccactc gccgaccacg
      721 acgatggcga tatcggattc ccgcgccaga tccaccgctc gccggaactc cgcctcgtcg
      781 tcgaagtttt cgggatcttc cggtgcgtta ccgggccaca tgtcgaacat cgacgggaac
      841 gtccgctgcg ccggccggat gccgggtgcg tactcgaccg tgactgcgtc gccgaccttc
      901 tcccggatgc cctccagcac ggtcacggtc tcttccaggt cgaactcaaa gcaccaggga
      961 ccgagcgtgt cgcgcctcga gtcagccaat ggaccgatca cggcgagcga gccccttgta
     1021 tcagcactga gcggcagcag gtcaccgccg ttcttcagca gcaccgccga gcgctcggcc
     1081 gcctcacggg caacctcccg gtgggcggga tcgcgcacga cctgccgtgc acggtcctcg
     1141 tcgacgaacg gttcctcgaa gagcccaagc cgaaccttgg cctccagcac tcgccggacg
     1201 ctggcgtcga acgtcgactc gtcgatacgc ccgtctgcga gggcctcggg caggtgggcg
     1261 taggccggat cagcgactgc catctccatg tcacagccgg cggtgaggcc ccgcacaccc
     1321 gcttcggcga gatccttggc gtaaccgtgg gttttcaggt tgcgcacagc gttggcgtcg
     1381 gagacgaccc aaccctcgaa gtcccacgtc tgccgcagca cctcgtcaaa gagccagggg
     1441 ttgccggtgg ccggcacgcc gttgagcgac atgtaggccg tcatgacgtt gccggcgccg
     1501 gcatcgaggg cggccttgaa cggcgggaag taggtgttcc agagctcgta gtcggagatg
     1561 ttcgcctcgt cgtagtcccg gccacccagt gcggcgccgt acccggcgaa gtgcttcggc
     1621 ccggcgacca cccgctccgg cgcgccgatc ccctcgccct gaaatccacg cacttgggcc
     1681 acggccatgg ctgccccgag aaaggcatcc tcccctgccc cctcgacgat gcgaccccaa
     1741 cgtgggtctc gtgcgatgcc caccgtcggc gcgaaggtcc agtggatgcc gacagcccga
     1801 gcctcccgcg cagcgaccac ctgaccgcgc tcgaccacct ccgggtccca cgaggacgcc
     1861 atcgcgatcg gcaccggcag gattgtccgg aatccgtgga tgacgtcgaa gccgaagatc
     1921 agtgggatat cgtggccgtt cccgtcgatc gccagcttct gcaaccggtt gaactcggcg
     1981 ggctcccgca cgaacagcag cgagcctgcc tcgcctcggg ccagggccgc ctcaaccaga
     2041 tccggctggt tgaactcgtc gtcgccctcg ccggcgggca tgtcccggag gaagccgaag
     2101 tagaagtact gggtgagctg ccccgccttc tcctcgaccg tcatctccgc cagcagggag
     2161 tccacccgct cgcggacctg ctcatcactc acccgggccc gcagcgccgc tcgatcatct
     2221 gccatgtcac tgattactcc agtcatgcag agccgaattg gtacaattgg cagagtatac
     2281 cctccgatag cctcagcatt tgggcacgtg gcacgatgcc accatcggcg gcgacaggca
     2341 ggagcacgta ccatgcgcta cggcacgaca accaaacgct cccctcgtga ggtgatcgac
     2401 caggcctgcg cgttcttcgg gccggaaggc gaacttggcc tccctgaggc ctcgacccgg
     2461 aacaacacca tcaccttctg cagcgaggca ggcttcctca gcatcgcctg cgaccctacc
     2521 ccggacggca ccgaggtcac ggtgctcagc cgcgaatacg atacctgggc cgagcagttc
     2581 atccgcaaga tcggctgaac cgagtcgtcc ctgaccctca agagccatta cgctccgaac
     2641 gcctattgac gtaagtccga tccgtcatag gataaggggg tcgtttcgcc attccctcca
     2701 ctcggtcagg aggacggcca tgaccacctc tgtccccgac ctttccatga tcaaacagcg
     2761 ccagcagcga gtctggtccg aaggcgattt cagcctcatg gcgatcccaa tcgtcgccgc
     2821 taggcgcaat gtctgacgag agactgagcc gagaggaaca tgccagtagc gacaccagca
     2881 cacctgcgaa aggtctctgg ctgacctgga tcggcgccgc cctgccgatg atcattctcc
     2941 cggtggtgat cgtcaccatt cccgttgcag tcgggaagtc catgcgactg acgagcgaca
     3001 agctcaccag ctggatcgtg gcgctctatg ccattccgag cgccctgagc ctcgcgttcg
     3061 ccctgctcta tcggcaacca ctcgttctca ccgggaacgt ctttttcatg atcttcatcg
     3121 cacggctcgg cggcgacatc gcgttcgccg agctagttgg cgcgtcgctc gtcgctgggg
     3181 cggtcgtcct cgccgtggtg gcgatgggcg tctcggacca cctcgcctcg ctcgtgccaa
     3241 cgccggtggt gctgggcctg ctggccggcg cggtcctccc attcgtcgtc gacctcttct
     3301 ccctgctggg cgaggaaccg gtcgccgtcg gcggtatggg attggcctac ctgctcggcc
     3361 agcgatttct gagtgcgagg cttccaccaa tcctgccggc actcatcgca gccagcctcc
     3421 ttgctcccgt taccaggaat gccggctcac tcccgactga cctccccctg gtgacggcaa
     3481 tggtcttcac gctccgattc acgctcgatg ggctcctcac cgtcatgcct gtgctcgtga
     3541 tcctgatctt gctgcagtcg aacctgccat cggtcatcta gagcggattt catgatggtt
     3601 gcttgtcttg gtgcggcagg gcgaagccgc agtggcggta gaagccggcg atgtc
//
LOCUS       DASYWL010000106         1529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_586989, whole genome shotgun sequence.
ACCESSION   DASYWL010000106 DASYWL010000000
VERSION     DASYWL010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586989"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(239..652)
                     /locus_tag="VGR22_03975"
     CDS             complement(239..652)
                     /locus_tag="VGR22_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127758.1"
                     /translation="MQSTNKRTAAVARQMTGASIEKRVDVEPGVLRGGVTGEVGAEAL
                     DEVDQGALVDHRAAVDVDQGSSWRHQTELASADHRAIYCTDAIGLSEIETRSVGRHAA
                     AEQVGRAGRGILSALPRQLVEGMGIEVSTTLGGLG"
     gene            763..1020
                     /locus_tag="VGR22_03980"
     CDS             763..1020
                     /locus_tag="VGR22_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127759.1"
                     /translation="MDPTRRVVRACMELANTSSRELDRLLDVYGPAVLSEQRAQVGTM
                     PHVAVGAMAKLGMDDPDGHEKVAERRSRRPFRAPVAIRQRY"
     gene            1192..>1529
                     /locus_tag="VGR22_03985"
     CDS             1192..>1529
                     /locus_tag="VGR22_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127760.1"
                     /translation="MSDTGKWDGSPSGDRTGSALGDIGSWTDQEVFRLWVSAMMELRK
                     REILRSFNTPTGDYAEWLVANDLGLTLENTSAAGHDAVDSEGTRYQIKARWLAPSRSS
                     RQLSIIRNLD"
BASE COUNT          286 a          528 c          462 g          253 t
ORIGIN      
        1 gcgcgacctc cgtcgaaagc ccctcgcgaa tgccatccgc gatcgagtcc tggaatcgcg
       61 gtagtcccac gaaccagacc agcaggcagc acaccagcgc gaggaacgca agcgcgccga
      121 atgaaaccag acagccgcgt ctccgcatga acaccgttcc ctccatcgtc cgccggcacg
      181 gcacgggagc gcccgagtcg tccggcccgc tgatcacgtc gcctttcgac gcgaggattc
      241 atcccagtcc gccaagtgta gtggatacct ctatgcccat accctccacc agctgtcgag
      301 ggagggcgga caggataccc ctgcccgcac gaccgacctg ctcggcggcg gcgtggcggc
      361 ccaccgagcg cgtctcgatt tcgctcaggc caatggcatc cgtgcagtag atggccctgt
      421 gatcggccga ggcgagctcc gtttgatgac gccaggagct gccctgatca acatcgactg
      481 cggcgcgatg gtcgacgagg gcgccctggt ccacttcatc gagagcctcg gcccctacct
      541 cgccggtgac accgccgcga agcacgcccg gctcgacatc gacccgcttt tctatagagg
      601 cgcccgtcat ctgccgggcc acggccgccg ttcgcttgtt ggtactctgc attggagaac
      661 gatgcgacct gcttccagcc tgactggacc ctccacgaat gaccaagtca ttgtggtagc
      721 ctgccgggaa tcggctcgcg ttcatgccaa ggaaacgcca ggatggaccc caccaggcgc
      781 gtcgtccgcg cctgcatgga actggcgaac accagctcgc gtgagctcga ccggttgctc
      841 gacgtctacg gtcccgccgt cctgagcgag cagcgcgcac aggtcggcac catgccccat
      901 gtcgccgtcg gcgcgatggc gaagctcggc atggacgatc ccgatgggca cgagaaggtg
      961 gcggagcgac gcagccggcg gccatttcga gcaccggtgg ctattcgcca gcgctactga
     1021 tgctcgacac ccgatccaga cgcttttgct gccctctgtt cagcaggttc cgtcgcgccc
     1081 gattcggtga cgcggcaaag gcctgttccc agtggaaaga ctccacccca cgaccctggc
     1141 tacgcccagc atcggatcga tgctcctcgc gctcgtgctc ggatggccgg gatgagcgac
     1201 acaggcaaat gggacggatc tccctctggt gaccgtaccg gcagcgctct cggcgatatc
     1261 ggtagctgga ccgaccaaga ggtcttccga ctctgggtat cggcgatgat ggagcttcga
     1321 aagcgcgaga tcctgcgctc attcaacaca cccaccggcg actacgccga gtggctcgtt
     1381 gccaacgacc tggggctgac cctggagaac acctcggcag ccgggcacga tgccgttgac
     1441 tctgaaggta ccagatacca gatcaaggca cgctggctgg cgccgtcgcg gtcgagccga
     1501 caattgagca tcatccgaaa ccttgatga
//
LOCUS       DASYWL010000107         4778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_568119, whole genome shotgun sequence.
ACCESSION   DASYWL010000107 DASYWL010000000
VERSION     DASYWL010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568119"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VGR22_03990"
     CDS             complement(<1..769)
                     /locus_tag="VGR22_03990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV2127761.1"
                     /translation="MNPRPDVIIIGGGISGTATAWELAQHGVRTTLLEADNLASMASG
                     WTLAGVRQSGRHPAELPLAQAAIKRWARLAEELDAAVEYRQDGNLRLALTEEDTPTIR
                     KVVEDGTSAGIEMRFLGSNDAVREIAPTLAPDIPAASWCPTDGHANPAETVRAFAEAA
                     RRAGADIRIATPVRALRYIGDRVTGVEIDGEVLEADTVVVAAGVYTPRLLAPLGIDVP
                     LTLVPAIQTVPVEHPQLRQVLGQAQGGVALRQQVSGHF"
     gene            complement(1099..1998)
                     /locus_tag="VGR22_03995"
     CDS             complement(1099..1998)
                     /locus_tag="VGR22_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2127762.1"
                     /translation="MAHAATADVADNTSGGPQVKLVSQRQRAWRTFRRNKSAVVGLGM
                     IIFVVVVALVAPLIAPHNPIEQSTINRLAEPTSDYLLGRDDFGRDVLSRIIYGARIAL
                     LVGVTSVLLGGILGTTMGIVAGYFGGRIGTVIMRVVDILLSFPDLITGLLVMAVLGAG
                     TFKLIIAIGLTITPRFARLAYGPTLSIKEKEFVEAALALGQRTQVILSRHILPNIAGE
                     LLVLASLWTAQAIRLEASLSFIGLGVPPPTPTWGQMIRDGTRYLTDYPLLSLAPGVAL
                     LFTVFAFNLVGDGLRDVLDPRSR"
     gene            complement(1998..2939)
                     /locus_tag="VGR22_04000"
     CDS             complement(1998..2939)
                     /locus_tag="VGR22_04000"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2127763.1"
                     /translation="MLRHIIRRLLLAIPTLFAVVTIIFLLVRVIPGDPARAALGDNAT
                     EEAVNTLREQLGLNDPIWVQYVNYIQDLLRGDLGTTLITRQPAWEQIRFVLPHTLELA
                     AAALVIALLLGIPIGIITAVKQNSPIDYTGRIVSLAGLSMPAFYLGLLLIYLFSARLG
                     WLPAIGAGDFSEPADNIKRLILPALSLGLIEMAFIARMTRSVMLNVLSADYVRTARAK
                     GLAEHVVLVRHAFRAGLVPIVSLVGIFVISLIGASVLTEEVFARPGLGKLMIGASKQR
                     DYTLLQAIMVLYAFIIVIVNIIVDLIYTFVDPRVRYD"
     gene            3160..4239
                     /locus_tag="VGR22_04005"
     CDS             3160..4239
                     /locus_tag="VGR22_04005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV2127764.1"
                     /translation="MVTTVVTGGGILGLATAWRLAQASQTVTVTERDALASAATYASF
                     GWLTSSSAIEPSVTYPLPYRELKVAGIAEYQRVARELGDASWLHVSGHVEWDMAEGGP
                     DRIQRKMVDLHSWGYSAELLPVRDLASLEPDLTPPAGLEAFVYYPNEGYIDPGAFVGL
                     LANQARELGTTIRTGVAVTGLLQERGRVTGVALTSGERIEADTVVACAGIGTPGILGQ
                     VGMHLPMLHSVGLVAVTGSSSARLRAVHHNEGMSIRPDGGGSRIIMRHWDFDAMVDVV
                     TPTQPHPPFVHDLLDRVVTVLPTLAGARVEAVRIAVRPIPGDHLPLVGYVPGVEGLYV
                     MVGHGATTHGLLVARLAAHEIAFGE"
     gene            complement(4409..>4778)
                     /locus_tag="VGR22_04010"
     CDS             complement(4409..>4778)
                     /locus_tag="VGR22_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127765.1"
                     /translation="HQAYHDLIRQDENPLTFYIAYRPNAHVYLSQFFTEGGGPTNFSK
                     FDVSEYVDQALATTDPDEQAQLWKEANIALQENFAGYGLYLVNQVYARAEYVDYGHEL
                     TAVVNLYPGITEKTSMSNGS"
BASE COUNT          812 a         1453 c         1715 g          798 t
ORIGIN      
        1 tgaagtgccc cgacacctgc tgccgcagcg cgacgccgcc ttgggcctgt ccgagcacct
       61 gccgcagctg ggggtgttcg accggcaccg tctggatcgc ggggacgagc gtgagcggga
      121 cgtcgatccc cagcggtgcg agcaggcgcg gcgtgtagac accagccgcg acgaccaccg
      181 tgtccgcctc cagcacctca ccgtcgattt ccacgcctgt gacgcgatcg ccgatgtagc
      241 gcagcgcgcg cactggcgtg gcgatacgga tatctgcgcc ggcgcgccgg gccgcctcgg
      301 cgaaggcacg tacggtctcc gcggggttgg cgtgaccgtc ggtcgggcac cacgatgccg
      361 ccgggatgtc cggcgcgagc gtcggggcga tctcccggac ggcgtcgttg gagccgagga
      421 atcgcatctc gattccggcg ctggtgccat cctcgaccac cttccgaatg gtcggggtgt
      481 cctcctcggt gagcgcgagg cgcaggttgc cgtcctgccg gtactcgacg gcagcgtcca
      541 gctcctcggc gagtctcgcc cagcgcttga tggccgcttg ggcaaggggg agttccgccg
      601 ggtggcggcc cgactgccgg acgccggcga gggtccagcc agaggccata ctcgcgaggt
      661 tgtcggcctc gaggagggtt gtgcgaacgc cgtgctgggc cagctcccat gcggtggcag
      721 taccactgat gccgccgccg atgatgatca cgtccggtct ggggttcaag agggatcctt
      781 tcgccgtcga tgagcaggca cgctctctgg ggaaccctac ccggaaacgg agcggtgggg
      841 aagggccatg catccctgtt ggtccgatgt gacagtgagc agggctcttc atggcggcca
      901 tattcgaccc gtgccttgcc atagcgaaaa cacgtcatcc tgagcggaaa actcccggcg
      961 cgaatggcgg ccttccgatt gcggcggcca gcctctttgt cggctcagag cgacactggc
     1021 cggttggtct ccaagttggc gtacacgtcg aatcccgccc atgtgcaggg cgggactacc
     1081 agatggcaac ggtttgcgtc agcgcgatct ggggtcgagc acgtcgcgaa gcccgtcgcc
     1141 gacgaggttg aaggcgaaga ccgtgaacag cagcgcgacg ccgggcgcga ggctgagcag
     1201 tgggtaatcg gtcaggtacc gcgtgccgtc ccggatcatc tggccccatg tcggtgtggg
     1261 tggcgggacg ccgagaccga tgaagctcag gctggcctcg aggcgaatcg cctgcgcggt
     1321 ccagagactg gcgagcacca gcagctcgcc agcaatgttc ggcaggatat ggcggctgag
     1381 gatcacctgg gtgcgctgcc cgagcgccag cgcggcctcg acgaactcct tctccttgat
     1441 ggagagggtc gggccgtagg cgagacgcgc gaagcgtggc gtgatcgtca gcccaatcgc
     1501 gatgatcagc ttgaacgtgc cggcgccgag cacggccatc accagcaggc cggtgatgag
     1561 atccgggaag gagagcagga tgtcgacgac gcgcatgatg accgtgccga tgcgcccgcc
     1621 gaagtagccg gcaacgatgc ccatcgtggt ccccagaatg ccgcccagca gcaccgaggt
     1681 cacaccgacg agcagggcga tgcgtgcccc atagatgatg cggctgagca catcgcgccc
     1741 gaagtcgtcg cgacccagca ggtagtcgga tgtcggctcg gcgaggcggt tgattgtgct
     1801 ctgctcgatg gggttgtgcg gggcgatcag tggcgccacc agcgcgacca ccaccacgaa
     1861 gatgatcatg ccgagcccga cgacggccga cttgttgcgc cggaacgtcc gccacgcccg
     1921 ctgtcgctgc gagacgagct tgacttgcgg gccaccggag gtgttgtcgg cgacgtccgc
     1981 ggtagctgca tgcgccacta gtcgtacctc acgcgtggat cgacaaacgt gtagatgaga
     2041 tcaacgatga tgttcacgat gacgatgatg aacgcataga gcaccatgat cgcctgcagc
     2101 agcgtgtagt cgcgctgttt gctggcgccg atcatcaact tcccgagtcc tggccgcgcg
     2161 aagacttcct cggtcagcac cgacgcgccg atcaggctga tcacgaagat gcccaccagc
     2221 gagacgatcg ggaccagtcc tgcacggaac gcgtgtctga cgagcacgac gtgctcggcc
     2281 aggccctttg cgcgcgccgt acgcacgtag tcggcggaga gcacgttgag catcaccgag
     2341 cgggtcatgc gggcgatgaa ggccatctcg atcagaccga gcgagagcgc tggaaggatc
     2401 agtcgcttga tgttgtcggc tggctccgag aaatcgcccg cgccgatggc cggcagccac
     2461 cccagcctgg ccgagaagag gtagatgagg agcaggccga ggtagaacgc cggcatggag
     2521 agcccggcga gcgagacgat gcggccggtg tagtcgatcg gggagttctg cttgacggcg
     2581 gtgatgatgc cgatcggaat gccgagcagc agggcgatga cgagcgcggc ggcggcgagc
     2641 tccagggtgt gcggcagcac gaatcggatc tgctcccatg cgggctggcg ggtgatgagc
     2701 gtggtgccca gatcgccccg caacagatcc tgtatgtagt tgacgtactg cacccagatc
     2761 gggtcgttca gcccgagctg ctcccggagg gtgttgacgg cctcttcggt ggcgttgtca
     2821 ccgagggccg cccgcgccgg gtcgcccgga atcacgcgca cgagcagaaa gatgatggtg
     2881 acgacggcga acagtgtcgg gatggccaac agcaggcgtc gaatgatgtg tcggagcatg
     2941 cggaggctcg cttgatctgc aagttgaaca gggtttcgaa cagcacgttg cccccaccat
     3001 agcatgaacg cgaggccaga cgcaggcgct gaaaggcgag actggcccgg ccagacaggc
     3061 tcggattgac gtctgacggg cgagacacta gcctgccgcc agcagcgagc agatgcgtcg
     3121 caggctgccg ccagcatggc gcgaggtcag gagtggccgg tggtaacgac ggtggtcaca
     3181 ggcggtggaa ttctcgggct ggcgacggct tggcggctgg cgcaggccag ccagacggtc
     3241 accgtgactg agcgtgacgc gctggcgagc gccgccacct acgcgagctt cggctggctt
     3301 acctccagca gcgccatcga gccgagcgtc acgtacccac tgccctatcg tgagctcaag
     3361 gtggccggga tcgccgagta ccagcgcgtg gcgcgggaac tcggggacgc gtcctggctg
     3421 cacgtcagtg gacatgtcga gtgggacatg gcagagggcg gcccggatcg tatccagcgg
     3481 aagatggttg atctccacag ctggggctac agcgccgagt tgctcccggt gcgcgatctg
     3541 gcgtccctgg agccggacct gacgcctccg gccgggttgg aagcctttgt ttactacccg
     3601 aatgaggggt acatcgaccc cggtgcgttc gtcgggctgc tggcgaacca ggcgcgcgag
     3661 ctgggcacga cgatccggac gggcgtggcg gtcacggggc tgctgcagga gaggggccgc
     3721 gtcaccgggg tagcgcttac gagtggcgag cggatcgagg ccgacacggt cgttgcctgc
     3781 gccggaatcg ggacaccggg aatcctgggg caggtcggga tgcatctgcc catgctccac
     3841 tcggtgggcc tggtggcggt gaccggatca tcgtcggccc gattgcgagc ggtgcatcac
     3901 aacgagggca tgagcatccg tcccgacggc ggcggcagca ggatcataat gcggcattgg
     3961 gatttcgacg cgatggtgga tgttgtcacg ccgacacagc cgcatccccc gtttgttcat
     4021 gacctcctgg atcgggtcgt cacggtgttg ccgactcttg ccggtgcgcg ggtggaggcg
     4081 gtgcgcattg cggtcaggcc catccccggc gatcacctcc cgctggtcgg gtatgttcct
     4141 ggggtagaag gtctctatgt gatggtcggg cacggggcga ccacacatgg tctgctggtg
     4201 gcgcggctgg cagcgcacga aatcgccttt ggcgagtagc tgccctcgcc tggagccgta
     4261 tcgccccgag cgtctcgtca cgccgctgac aggcgtcggc tgatcaggag ccggatgcac
     4321 gacgggagcg aaatgacgag cccgcccggt gtctagccgg gtgggctcgc agtaaggcgc
     4381 gctggcggat cagttgtcag ctgcggcgct agctgccgtt gctcattgag gtcttctccg
     4441 tgatgcccgg gtagaggtta accaccgcgg tgagctcgtg gccgtagtcc acgtactcgg
     4501 cgcgcgcgta gacctggttc accaggtaga ggccgtaccc cgcgaagttc tcctggaggg
     4561 cgatgttggc ctccttccag agctgagcct gctcatcagg gtccgtggtg gcgagcgctt
     4621 ggtccacgta ttcgctcacg tcgaacttcg agaagttcgt cgggccgccg ccctcggtga
     4681 agaactgcga caggtagaca tgcgcgttcg gccggtaggc gatgtagaag gtcagcgggt
     4741 tctcgtcctg gcggatgagg tcgtgatagg cctggtgc
//
LOCUS       DASYWL010000108         2951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_418135, whole genome shotgun sequence.
ACCESSION   DASYWL010000108 DASYWL010000000
VERSION     DASYWL010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418135"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            596..1789
                     /locus_tag="VGR22_04015"
     CDS             596..1789
                     /locus_tag="VGR22_04015"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEV2127766.1"
                     /translation="MERTRERASIHPGPPGSFHVAGQFAQTHHPASIPSARARIVGID
                     VARGIAILGMMAVHILDPGPFPWIDPMPENWDGVFAGRSSILFAIIAGISVALMTGRD
                     RPVTGGHLVRARTRILVRAVVLFAVGGVLNMLVHNIAVILEYYAVLFMLALPFLAWPP
                     RRVFQLVAALVTVLPPIYMIVINLALREEIGRPSTMTRLMLTGNYPAIIWIIFILIGL
                     GVGRLELTSRQIQRRLLAIGTTMLIVGYGAGQAAQRLFPPRPRADDGLPGRFDLDRMA
                     TMEPHTGAWFEVVGSSGVALAVLALCLMATPRSGRLLTPLSAAGSMALSIYVLHVVSM
                     VWIWPALTEHNQYQFYLGTVVVSLVFASVWAHLFGRGPLERLLTTISRRTATAKRPAV
                     VQVAG"
     gene            complement(2496..>2951)
                     /locus_tag="VGR22_04020"
     CDS             complement(2496..>2951)
                     /locus_tag="VGR22_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127767.1"
                     /translation="EWFRCPGCLSRRAVLFSVGGVFRCRACHDQAYTSTREDETERAN
                     RRIVALQRKLKAALGCHLFHVPAKPKGMHWRTFERLVSQLLTEHHRRDALYEAGLIAL
                     LRRTDTLLAWSERIPSACENDRNPHALLVALWAVGTSKDRTPYAMRDTP"
BASE COUNT          493 a          993 c          921 g          544 t
ORIGIN      
        1 gctaagcacc gccgtgcgct tggcgtccga caggcgttcc catcgcgcct ggtcgaggcg
       61 ctgcttgacc ccgcgccggt gctccgacgg gcacgcgtcg tggtaggctg ccctccggct
      121 cggtaccacg ctgtaccagc acgtcgccac accaccggca gggcacgtag gccacgcgac
      181 aggtccaccc ccgccggcgg aaccgcgcgg gcattgcgga cggtgctggc gttcacccct
      241 agccggcgcc cgaaccggct acaggttctg ggccgacccc tgatgcccct gccgcctcgc
      301 gaagcgggct gatccctccg ggtgcacggg tcaaatgcgt tggagcagac cgactggaca
      361 actgtggagt tcaatgcgaa atacttgctc aaggcagcaa cgctcggggt gcgggacaaa
      421 gctgtcgcag gttcagcgct ccgcctccgc gacgccgatc tggtggcgca ccagtctgct
      481 tgcgcacgca tcccgatagc cgcgccttgc ctcatgactg gttgtaagga tcgatgccgt
      541 ttcagggcaa tcgttccagc acgaggaagc gccgtcgtca tggggagtac cgatcatgga
      601 gcgcacgaga gaacgagcca gtatccaccc aggaccaccg ggttcattcc acgtcgctgg
      661 ccagtttgcc cagacgcatc accccgcgtc gataccttcg gccagagctc ggatcgtcgg
      721 catcgacgtc gcgcgcggca tcgccattct cggaatgatg gcggtgcaca tcctcgatcc
      781 cggcccgttt ccgtggatcg atccgatgcc ggaaaactgg gacggagtct tcgccgggcg
      841 ctcgtcgatc ctgttcgcca tcatcgccgg catctccgtc gcgctcatga cgggccgcga
      901 ccgaccggtg accgggggcc atctcgtgcg cgctcgcaca cgcatcctgg tgcgggccgt
      961 ggtgctcttc gccgtcggcg gtgtcctcaa catgctggtt cacaacatcg ccgtcattct
     1021 cgagtactac gcggtcctct tcatgttggc gctcccgttc ctcgcctggc caccacgacg
     1081 ggtgttccag ctggtggccg cgctcgtgac cgtgctgcca ccgatctaca tgatcgtgat
     1141 caatctcgct ctcagagagg agatcggcag gccgagcacg atgactcgcc tgatgctcac
     1201 cggaaactat cccgccatca tctggatcat cttcatcctg attggtctcg gcgtcgggcg
     1261 cctcgagctg acctcacgcc agattcaacg acgcctcctc gcgatcggca cgaccatgct
     1321 gatcgtcggc tacggcgcgg gccaggcagc gcagcgcctg ttcccgccac gcccacgcgc
     1381 ggacgacggg ctgccgggac gcttcgatct cgaccgcatg gcgaccatgg agccgcacac
     1441 gggggcgtgg ttcgaggtag ttggctcgag cggggtcgcc ctcgcggtcc tggcactctg
     1501 cctgatggca acgccacgat cgggccggct gctcaccccg ctctcggcag cggggtcgat
     1561 ggcgttgtcc atctacgtct tgcatgtcgt ctcgatggtc tggatctggc ccgcgctcac
     1621 cgagcacaac caataccagt tctatctcgg gacggtcgtg gtctcgctcg tgttcgcgag
     1681 cgtctgggcg cacctgttcg gcagagggcc gttggagcgg ctgctgacga ccatatcccg
     1741 gcgcactgcc acggcgaagc gaccagccgt cgttcaggtc gcgggctagc aaagccgttt
     1801 gggcctgacc gtcgctcagt gaggccggga gtgtaccctc gctcccctgg agggctgctc
     1861 atggcgagcg cggttgcatg gcgactatgc ggcgagctct tgatcagcgc gacggtcaac
     1921 cgacagtggc gcaaccgcgc tgatgcatgg ccgtctcgag gatcacccgg atgccggtgg
     1981 cgccgtccgc atgcgccatg gcgtctcgct gcagccaggt tcatgcactt tcgggaacca
     2041 agagcctgcg ccaataggtg agcggggaca ttgctaggca gggtcacgga gatgctcctc
     2101 acacggcaaa cctcgagacc tgcccgcatg atgcaagtat ccgtgctcat gttctccccc
     2161 agcactcatg cctattggca caagctctta ttgcatgtca ataggggagg cgtgggttat
     2221 atctggtggg ttatatctgt atgaagaacc gggttcgaag accctcggga caagaatgag
     2281 gcatcaccgt gacggccgtt tcacccacca cgtagtcctc acctaaagga ccaccacatc
     2341 gcttcggcgt cacgactacg agcctttacg ctaacgacaa ggccaatacc gacttatgac
     2401 ggatcggtat agactagcag ccgggggcag ctgacagggg gatccagcat gggcaatcgc
     2461 agagccgact ccgtgtttcg atcgagcgcg gcggtctacg gggtgtcccg catcgcatag
     2521 ggtgtccgat ccttggatgt tccgacggcc catagggcta ccagcagtgc atgggggttt
     2581 cggtcattct cgcaggcact cggtatcctc tctgaccacg ccagcaacgt atcggtgcgc
     2641 cgtagcagcg cgatcagacc ggcctcgtac agcgcatccc gccggtggtg ctcggtcagc
     2701 agctgcgaca ccagccgctc gaacgtgcgc cagtgcatcc ccttcggttt cgccggcaca
     2761 tggaacaggt ggcagcccag cgcggccttc agcttgcgtt gcagcgccac gatgcgccgg
     2821 ttcgcccgct cggtttcatc ctcgcgggtc gaggtgtacg cctggtcgtg acacgcccgg
     2881 cagcggaaca cgcccccgac gctgaagagc accgctcgcc ggctcaggca gccggggcac
     2941 cggaaccact c
//
LOCUS       DASYWL010000109        10487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_42344, whole genome shotgun sequence.
ACCESSION   DASYWL010000109 DASYWL010000000
VERSION     DASYWL010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10487
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42344"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1012
                     /locus_tag="VGR22_04025"
     CDS             <1..1012
                     /locus_tag="VGR22_04025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEV2127768.1"
                     /translation="IRGTGDGSGKGKVVLARADMDALPIVEENEVDYRSKNDGVMHAC
                     GHDAHTAILLAVARVLMDRRDQFAGTVKLCFQPSEETGPGGAKWMIEQGVMQDPPVDA
                     CFGLHVWQNEPVGTVLVSDGPVMANSDRFEIRIQGKGGHGAMPHGCVDPIVVGMQIVG
                     ALQTLVSREVDPTEAAVVTVGEFHAGHAPNVIPDTATIRGTVRSFAPEVQDLLERRII
                     ELAEGIGASMRAEVKAEYRRGVPATVNDPAMAEIARQAAIEVVGEENVRPQIPTMGGE
                     DMSFFLLEKPGCYFWVGTNNEDRGLTWGHHHPRFDIDEEALGVGVETMVRTLTRYLAQ
                     DG"
     gene            1216..2406
                     /locus_tag="VGR22_04030"
     CDS             1216..2406
                     /locus_tag="VGR22_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEV2127769.1"
                     /translation="MVAADVRRDVDEILPGVVADRRHLHQNPELAFEEHETAKFVAER
                     LEALGVEDIRTGVGRTGVTGLIRGTKGEGRCVLFRADMDALPVDEENEVDYKSTVRGR
                     MHACGHDAHTAMLLGTARVLTGMRDQFAGTVKLLFQPAEEVPPGGAIEMIRDGALEDP
                     HVDAVFGLHVAAELPAGKIGVKVGAASASSDRFRIVIQGRGGHAARPNLAIDPVVVGS
                     HIVVALQTLVSREVDPTGAAVVTVGSIVSGEAFNVIPDTAEIKGTVRTLDPEVREQMA
                     RRVPEIAVGVGKAMRAEVDVNYFKGYPSMVNDEEMTELVREAAIDVVGAENVVDVPVG
                     MGGEDFARFLLERPGSFFRVGTRSEERGITYGHHHPRFDVEEEGMGAGVATSVETILR
                     YLNR"
     gene            2499..3584
                     /locus_tag="VGR22_04035"
     CDS             2499..3584
                     /locus_tag="VGR22_04035"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2127770.1"
                     /translation="MIRAGIIGCGDVARRAYIPDIQELADRITVVAAFDTVADRAGDI
                     VGMFPGATAYTSIEEFLDHEGGGAIDLVFNLTPAPLHWQITAQVLEARYHVYSEKPIA
                     STVEEASELVRIAEHRGRELICAPATMVTGRFRWMKQFLDDGELGRTIAIKAHIGNMG
                     PAAWGEYFGDPRVFYQQGVGPLIDTGVYMLHVMTGLLGPAKRIQAVGGIAIPERGTTI
                     PRLQGEKIEVTTPDLFSINVDFGGETYGHLYSSFAIPQSKAPYFELYGEQGAISVDRV
                     PWFHGNGPSDLFLLDRPLAGWRMVLPDPIRTNSILGSGILHAIQHLEDGEPLVMTAAH
                     ATHVLEIMNAAHESAQTGEAIDVETTL"
     gene            3660..4418
                     /locus_tag="VGR22_04040"
     CDS             3660..4418
                     /locus_tag="VGR22_04040"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEV2127771.1"
                     /translation="MKMDQVAVQMYTLRQHATEDLDGTLWRLSDIGYKKVEFAGFYDN
                     AAEDVAAMLERHDLTAPAAHIPYARFESEFDNVIADAQAVGLEWAIVPSLPRREIDEG
                     LAQEYISKLNSFALLAEEAGLKFAYHNHAFEFENEYGDGTTFFDRLAAGTDPDKVKLE
                     LDAFWVNRGGFDPARILRDHGDRVRLVHLKDAARGHADSDIPFGEGALDWEAILGAAD
                     AAGVEYYVVEQDNPGDAFEDVTVALRNAEKWSES"
     gene            4415..5752
                     /locus_tag="VGR22_04045"
     CDS             4415..5752
                     /locus_tag="VGR22_04045"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HEV2127772.1"
                     /translation="MSDGGQFPEGFVWGAATSSYQIEGAVREDGRGPSIWDTFSHTPG
                     KTRGGDTGDVADDHYHRWHQDVMLMRDLGLQAYRFSVAWPRVIPDGGRQVNEAGLDFY
                     DRLVDGLLESDIEPWLTLYHWDLPQALADRGGLTNSEVAGAFVTYTEAVARRLGDRVK
                     RWITINEPWVAGIMGYWWGVHAPGETSREAGLAASHNLLRMHGMAVEVLRGMVPDVKA
                     GITLNLTPVYPIRDTEEDREAARLKDGEQNRWFLDPVFRGGYPEDMVREYGDDVPEIA
                     DGDMALISRPTDFLGINYYNPTYVSADEDAPQPDGERTEMGWLVEPEGLKDILTWMHE
                     EYGPQEIAITENGAAYDDPAPVDDRVHDPKRIAYFHAHLLAAKEAIDAGVPLRAYFAW
                     SLMDNFEWAEGYSKRFGIVHVDYSTQERTLKDSARWYANVIEANAVLGEDVID"
     gene            complement(5839..6486)
                     /locus_tag="VGR22_04050"
     CDS             complement(5839..6486)
                     /locus_tag="VGR22_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEV2127773.1"
                     /translation="MVARSLAIHPIWILAALALLAASLASPAAGQTPTVPVDEPSFET
                     HGSLPWEEPPGATPFVVEEVVDGDTIRVIEDADRNGPDPWWELVRIIGIDTPETESPY
                     TEEECYGEEASEFLSELLPQGTTVYLQVDNDPDIPDDREITDEGMELDDYDRWLAHAY
                     LRDGEDYYLLSEVIVLGGYAEVPGYEGNTYFVKELDEAEEIAREENRGMWGACDA"
     regulatory      6598..6694
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6780..7403
                     /gene="thiE"
                     /locus_tag="VGR22_04055"
     CDS             6780..7403
                     /gene="thiE"
                     /locus_tag="VGR22_04055"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV2127774.1"
                     /translation="MDRQLHLVTDPALDAVEQVRRIEAAIPHGIDTVHVRMPSGVACE
                     VYELAVVLQASLLDRDIPLIVNDRVDVALAVNAKGVQLGVRSLPVRAVRQMLGPEAPI
                     GASVHSVDEARAAEREGATWVSFGHVYETASHLDEPPRGFEVLHDVVDAVRVPVIAIG
                     GITAERVPEVLGAGASGIAVISAILGAEDIAGATRSLREALDAHFDV"
     gene            7476..8495
                     /gene="thiS"
                     /locus_tag="VGR22_04060"
     CDS             7476..8495
                     /gene="thiS"
                     /locus_tag="VGR22_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEV2127775.1"
                     /translation="MRILLNREEAQVEEGATIAAVVEEKALPARSVIVAVNDDVVERE
                     QWPTHELQEGDSVEIVRAVSGGDHTMTSNDTLTIAGREFTSRLFLGTGKYADDTMMMA
                     ALQASGTELVTVAVRYMKLDGSEQNTNILDLLSEARMHLLPNTAGAYTVQDALKMARL
                     AKAATGTDWIKLEVIGDQASLWPDVTATVEATRILVDEGFVVLPYTSPDVVIARQLEE
                     AGAATVMPLASPIGSGQGMQDWGSIQRIKEAVSVPVVVDAGIGAPSDAAIAMELGADA
                     VLVNTAVAKATNPPVMAEAMKHGTIAGRLGYLAGRMPRQQHAQPSSPTGGVPTALASA
                     TNGRR"
     gene            8721..9971
                     /locus_tag="VGR22_04065"
     CDS             8721..9971
                     /locus_tag="VGR22_04065"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2127776.1"
                     /translation="MDRFGKRRDSLTVDSDNSLASARDDWQCAGWQVGGGYFWYYAGI
                     GAFIPFVALHFRELGYSGPQVGLLTALPSVGVAIGGPLAGAVADALSVHRWVLRIALL
                     VGTVLALLASMIEAFPFMLAAIAPLALVLSPVPSMMDSYAVTASERANRSYGALRVWG
                     SVGYMAAVLVMGRVMGERVSALLFVGYALCLGLTLLTVFALPPLAERRAQPLLTGIHA
                     ATANRPLMTLLLVAYLLSIGTAAMNIYLGVHLEEIGGSASLIGVAFAISAASELPIVA
                     FGSWFLVRLGPVRLAALAIVVYALRFAGYSAITVPEWVLGIQLFHGLSYGAFLIASVT
                     LAHRLAGQEQASTAQALLTAMSFGFGSITGSLLGGVLLDVVGTAGLFGGSAGLMVVTL
                     AVLAIGNRLVGLGGDKVAGAHASG"
     gene            9964..>10487
                     /locus_tag="VGR22_04070"
     CDS             9964..>10487
                     /locus_tag="VGR22_04070"
                     /inference="COORDINATES: protein motif:HMM:NF015764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2127777.1"
                     /translation="MANPARVSAVWDRADLKVAGGYFAYFAAVGALMPYVALYFRGMG
                     FSGLQVGVLTALPSVGLAVFAPLWGAVADAMGVHRLLMRGALGLAIAVAFLTTQASTF
                     MSMLLLFGLLAFVSVPVAPLMDSYGVTASEHTRRSYGGIRVWGSIGYMVSVLIVGWLM
                     GDETSSLLLVAHAA"
BASE COUNT         1734 a         3296 c         3676 g         1781 t
ORIGIN      
        1 gattcgcggc accggcgatg gttccggcaa gggcaaggtc gtgctggcgc gggccgacat
       61 ggacgcgctg ccgatcgtcg aggagaacga ggtcgactac cgctcgaaga acgatggtgt
      121 gatgcatgct tgcgggcacg atgcgcatac cgccatcctc ctcgcggttg cccgggtgct
      181 gatggaccgg cgggaccagt ttgccggcac ggtcaagctc tgcttccagc cgagcgagga
      241 gaccggcccg ggcggcgcga agtggatgat cgagcagggg gtcatgcagg atccaccggt
      301 cgacgcctgc ttcggactgc acgtatggca gaacgagccg gtcggaacgg tgctggtcag
      361 cgacggaccg gtgatggcga actcggaccg cttcgagatc aggatccagg gcaagggcgg
      421 ccacggcgcg atgccgcacg gttgcgtgga tccgatcgtc gtcggcatgc agatcgtcgg
      481 cgcgctgcag acgctcgtct cccgcgaggt cgacccgacc gaagcggcgg tggtgacggt
      541 cggagagttc cacgccggac acgcgccgaa cgtcatcccg gataccgcga cgatacgtgg
      601 cactgtccgc agcttcgcac cagaggtgca ggatctgctg gagcggcgca tcatcgagct
      661 ggcggagggc atcggtgcgt cgatgcgggc cgaggtcaag gccgagtacc ggcggggcgt
      721 gccggcgacg gtcaacgacc cggcgatggc cgagatcgcg cggcaggcgg cgatcgaggt
      781 ggtcggcgag gagaatgtca ggccgcagat cccgacgatg ggtggcgagg acatgtcgtt
      841 cttcctgctc gagaagccgg gctgctactt ctgggtgggc acgaacaacg aggatcgcgg
      901 cctgacgtgg ggacaccatc acccgcgctt cgatatcgac gaggaggcgc tcggcgtcgg
      961 cgtcgagacg atggtgcgca cgctgacgag gtacctggcg caggacgggt aatggtcgcg
     1021 catccagtag gggcctcgtt cttaacccct cgacggaagg gcgtcaaccg ggcgcggtag
     1081 cgagtgctcg ctgtcatcct gagcgaggtg cgttagcgcc gagacgtgtc gaaggatgac
     1141 aggcatattg aaggttttga tcagcctgta ttcgctgctg atgacagacc aaatggatac
     1201 gaagggacac gaatcatggt tgcagcggac gttcgcaggg acgtggacga gattctgccg
     1261 ggggtggtcg ccgaccggcg gcatctccac caaaacccgg agctcgcctt cgaggaacac
     1321 gagacggcga agttcgttgc cgagcggctc gaggccctgg gcgtggagga catccgtacc
     1381 ggcgtagggc gcaccggcgt caccgggctc atccggggca cgaagggcga gggcaggtgc
     1441 gtgctcttcc gtgctgacat ggatgccctg cccgtcgacg aggagaacga ggtcgactac
     1501 aagtcgaccg tgcgcggcag gatgcatgca tgcgggcacg acgcccatac ggcaatgctg
     1561 ctcggtaccg cccgggttct gacgggcatg cgtgaccagt tcgccggcac ggtcaagctg
     1621 ctcttccagc cagcggagga ggttccgccc ggcggcgcga tcgagatgat ccgcgacgga
     1681 gcgctcgagg acccgcatgt cgatgctgtc ttcgggctgc acgtcgccgc cgagctaccg
     1741 gccggaaaga ttggtgtcaa ggttggggct gcgtcggcgt ccagcgatcg tttccgtatc
     1801 gtgatccagg gcaggggcgg gcacgccgcc cgcccgaacc tcgcgatcga tccggtggtg
     1861 gtcggatcgc acatcgtcgt cgcgctgcag acgctcgtct cccgcgaggt cgacccgacc
     1921 ggggccgccg tcgtgacggt cgggtcgatc gtctcgggcg aggcgttcaa tgtcattccc
     1981 gacactgccg agatcaaggg caccgttcgt acgctcgatc ccgaggtgcg tgagcagatg
     2041 gccaggcgcg tgccggagat cgccgtcggc gtcggcaagg cgatgcgcgc cgaggtcgac
     2101 gtcaattact tcaagggcta ccccagcatg gtcaacgatg aggagatgac cgagctggtc
     2161 cgggaggccg cgatcgatgt cgtcggcgcg gagaacgtcg tcgacgtgcc cgtcggtatg
     2221 ggcggcgagg acttcgcgcg cttcctgctg gagcggcccg gcagtttctt ccgcgtcggc
     2281 acgcgcagcg aggagcgtgg catcacctat gggcaccatc atcctcgctt cgatgtcgag
     2341 gaagaaggaa tgggcgccgg agtcgcgacc tcggtggaga cgatcctgcg ctacctcaac
     2401 cggtagaacc gttcgccgca gggtgggaat gtgagcgccc cggctctgcc cggggcgctc
     2461 accatgctgg agcgccatac gaaaggaccc acaggtagat gatccgcgca gggattattg
     2521 gatgcggtga cgtggcgcgt cgcgcctaca tccccgacat tcaggagctg gccgatcgca
     2581 tcacggtggt cgccgcgttt gataccgtgg ccgaccgtgc cggggacatc gttggcatgt
     2641 tccccggtgc gacagcctac acctccatcg aggagtttct cgatcacgag gggggcgggg
     2701 cgatcgatct cgtcttcaac ctgaccccgg cgccgctgca ctggcagatc acggcgcagg
     2761 tgctggaggc caggtaccac gtctactccg agaagccgat cgcctcgacc gtcgaggagg
     2821 cgagcgagct ggttcggatc gccgagcatc ggggtcgcga gttgatctgc gcgccggcga
     2881 ccatggtgac gggtcggttc cgttggatga agcagttcct ggacgatggc gagcttggcc
     2941 gcacgattgc gatcaaggcg cacatcggca acatggggcc ggcggcgtgg ggcgaatact
     3001 tcggtgatcc acgcgtcttc taccagcagg gcgtcggtcc gctcatcgac accggcgtct
     3061 acatgctgca cgtcatgacc ggcctgctgg ggccggcgaa gcgcatccag gccgtgggcg
     3121 ggatcgccat ccccgagcgc gggacgacca ttccccgctt gcagggcgag aagatcgagg
     3181 tgacgacgcc ggacctcttc tcgatcaacg tcgatttcgg cggcgagacc tacgggcatc
     3241 tctactccag cttcgccatt ccccagagca aggccccgta ctttgagctc tacggtgagc
     3301 agggggccat cagcgtcgac cgcgtgccct ggttccatgg caacggcccg agcgacctct
     3361 tcctgctcga ccggccgctg gcgggttggc ggatggtgtt gcccgacccg atccggacca
     3421 acagcatcct cgggtcgggc atccttcatg cgattcagca cttggaggat ggcgagccgc
     3481 tcgtgatgac tgcggcccat gccacgcacg tgctggagat catgaacgcc gcgcacgaat
     3541 ccgcgcagac cggcgaggcc atcgacgtcg agacgacgct ctgagttgcg aaatagcggc
     3601 aatgattctg catcaggaag gaatgggcat ggtgcccggg aacgggaagg gaaaacggca
     3661 tgaagatgga tcaagtcgcg gtgcagatgt acacgctgcg ccagcatgcg acggaggacc
     3721 tcgacgggac gctctggcgc ctgtcggaca tcgggtacaa gaaggtcgag ttcgcaggct
     3781 tctacgacaa cgccgccgag gacgtcgccg cgatgctgga gcggcatgac ctgacggcgc
     3841 cagccgccca catcccgtac gcgcgcttcg agagcgagtt cgacaacgtc atcgccgatg
     3901 cgcaggcggt agggctcgag tgggcgatcg tgccgtccct ccccaggcgg gagatcgacg
     3961 agggtctggc acaggagtac atcagcaagc tcaactcctt tgcgctgctg gccgaggagg
     4021 ccgggctgaa gttcgcctac cacaaccatg cattcgagtt cgagaacgag tacggcgacg
     4081 gcacgacatt cttcgaccgt ctcgccgccg ggactgaccc cgacaaggtc aagctggagc
     4141 tggacgcctt ctgggtgaac cggggcgggt tcgatccggc gaggatcctg cgcgaccacg
     4201 gcgatcgcgt gcggctcgtc cacctcaagg acgctgcccg cgggcacgcc gactccgaca
     4261 ttcccttcgg cgagggggcg ctcgactggg aggcgatcct cggggcggcg gacgcagccg
     4321 gcgtcgagta ttacgtcgtc gagcaggaca acccaggcga tgccttcgag gacgtcaccg
     4381 tggcgctgcg taacgccgag aagtggagcg agtcatgagc gatggaggcc aattccccga
     4441 ggggttcgta tggggcgcag cgacctccag ctatcagatc gagggtgcgg tccgcgagga
     4501 cgggcgcggt ccctccatct gggatacctt cagccacacg ccgggcaaga ctcgaggcgg
     4561 tgacaccggc gacgtcgccg acgatcacta ccaccgatgg catcaggacg tcatgctgat
     4621 gcgcgatctg ggcctgcagg cctaccggtt ctcggttgct tggccgcgcg tgattccgga
     4681 cgggggccgg caggtcaacg aggcggggct cgacttctac gatcgcctcg ttgatggcct
     4741 gctggagtcc gacatcgagc catggctgac cctctatcac tgggatctgc cgcaggcact
     4801 ggcggacagg ggtgggctga ccaacagcga ggtcgccggc gcgttcgtta cctacaccga
     4861 ggcggtcgcg cgccgactgg gggaccgcgt caagcggtgg atcaccatca acgagccgtg
     4921 ggtcgccggc atcatgggct actggtgggg cgtgcacgcg ccgggcgaga cctctcgcga
     4981 agccgggctg gcggcgtcgc acaatctgct gcggatgcac ggtatggccg tggaggtgct
     5041 gcgcggcatg gtgcccgacg tgaaggcggg cattacgctc aacctgacac cggtctaccc
     5101 gatccgtgac accgaggagg atcgagaggc ggcgcgactc aaggacggcg agcagaaccg
     5161 ctggttcctc gacccggtct tccgtggagg gtatccggag gacatggtcc gagagtacgg
     5221 tgatgacgtg ccggagatcg ccgacggcga catggcgctg atctcgcggc cgaccgactt
     5281 cctcggcatc aactactaca acccgaccta cgtctccgcc gacgaggacg ccccgcagcc
     5341 ggacggcgag cgcacggaga tgggctggct ggtcgagccg gaggggctga aggatattct
     5401 cacctggatg catgaggagt atgggccgca ggagatcgcg atcaccgaga acggcgcggc
     5461 ctacgacgac ccggcgccgg tcgatgatcg ggtccacgac ccgaagcgga tcgcgtactt
     5521 ccacgcgcac ctgctcgccg cgaaggaggc gatcgatgcc ggcgtcccgc tgcgcgccta
     5581 cttcgcctgg tcactgatgg acaacttcga gtgggccgag gggtactcca agcggttcgg
     5641 catcgtccac gtcgactaca gcacacagga gcgcaccctc aaggacagcg cccgctggta
     5701 tgccaacgtc atcgaggcga acgccgtcct cggcgaggac gtcatcgact gacgtgtggt
     5761 cggaaacgat cttgtagccc cgccctgaaa atgggcgggg atcgccgcac gaatggccgg
     5821 cacaaggccg gtgctacatc aggcgtcgca ggcgccccac atcccgcgat tctcttcccg
     5881 cgcgatctct tccgcttcgt cgagctcctt gacgaagtag gtgttgccct cgtagcccgg
     5941 tacctcggcg tatccgccca gcacgatgac ctccgagagc agatagtaat cctcgccgtc
     6001 ccgcaggtag gcgtgcgcca gccagcggtc gtagtcgtcc agctccatgc cctcatcggt
     6061 gatctcgcga tcgtccggga tgtccggatc gttgtcgacc tgcaggtaga cggtcgttcc
     6121 ctgcggcagc agttcgctca ggaactcgga cgcctcctcg ccgtagcact cctcctcggt
     6181 gtaggggctc tccgtctccg gagtgtcgat gccgatgatg cggaccaact cccaccacgg
     6241 gtcaggcccg ttccgatcgg cgtcctcgat caccctgatg gtgtcgccat cgaccacctc
     6301 ctcgacaacg aacggcgttg cgccgggcgg ttcctcccac ggcagcgaac catgggtttc
     6361 gaagctgggc tcgtcgacag gtaccgtcgg tgtctgtccg gcggccggcg atgccagaga
     6421 agcggccagc agcgcgaggg cggcgaggat ccagattggg tggattgcaa gcgatcgagc
     6481 gaccatgcgt cctcctcaat ggggtgctct catcttccac gtaaacggtg ctacgccgaa
     6541 aaccctacgc cagcgtcagg gacgattcgc tgcaagacac cgggtggtat gatgccagca
     6601 acgcgccagg ggtgcctcga acatcgaggc tgagatgaca ccctcaggac ctgatccggg
     6661 cagtaccggc ggaggaaggt gcggagcaga acttcattct gagccgtgac acccctcgac
     6721 gccagtgtat ccacgaaact ggcgcgtcga gggtttattc gtgcaccttg gagatcgcta
     6781 tggatcgtca gctccatctc gttaccgatc cggcactgga cgcggtggag caggtacgac
     6841 ggatcgaggc ggctatccct cacggaatcg acaccgtaca cgtgcgcatg cctagtggcg
     6901 tcgcgtgtga ggtctacgag cttgcggtcg tcctgcaggc atcgctcctg gaccgtgaca
     6961 tcccgctcat cgtcaacgac agggtcgatg tcgcgctggc ggtcaacgcg aagggcgtcc
     7021 agcttggtgt ccggagcctg ccggtgcgag cggtgcgtca gatgctcggc ccggaagcgc
     7081 cgatcggtgc gtcagttcat tcagtcgacg aggcgcgcgc tgcggagcgc gagggcgcga
     7141 cgtgggtctc gttcgggcat gtctacgaga ccgccagcca cctcgacgag ccgccacgtg
     7201 gcttcgaggt gctgcacgac gtggtcgatg cggtccgggt gccggtgatc gccatcggcg
     7261 gcatcaccgc cgagcgggtg ccggaggtgc tcggtgccgg ggcgagcggg attgcggtca
     7321 tctcggccat tctcggcgcg gaggacatcg ccggggcaac gcggtcgctc cgggaggcgt
     7381 tggacgccca tttcgacgtg taggggaggg cccccgcgcc ctcccgtttt ccggcctgcg
     7441 tatgttccga agcgaaggtc cgaaagggaa atcacatgag gattctgctg aaccgcgagg
     7501 aggcccaggt cgaggagggc gcgacgatcg cggcggtcgt cgaagagaag gcactgccgg
     7561 cccgctcggt gatcgtcgcc gtcaatgacg acgtggttga gcgcgagcag tggccgaccc
     7621 atgagctgca ggagggggac agtgtcgaga tcgttcgcgc cgtctccgga ggagaccaca
     7681 ccatgaccag caacgacacc ctgacgatcg ccgggcgcga gttcacgtcg cggctctttc
     7741 tcggcacggg caagtacgcg gacgacacga tgatgatggc cgccttgcaa gcctccggca
     7801 cggaactggt gacggtggcg gtgcgctaca tgaagctgga cggctcggag cagaacacga
     7861 acatcctcga cctgctcagc gaggcgagga tgcacttgtt gccgaacaca gcgggcgcct
     7921 acaccgtcca ggacgcgctc aagatggccc ggttggcgaa ggcggcgacg ggcaccgact
     7981 ggatcaagct cgaagtgatc ggcgaccagg cgtcgctctg gccggacgtc accgcgacag
     8041 tcgaggcgac gcgcatcctc gtcgatgagg ggttcgtggt gctgccgtac accagccccg
     8101 acgtcgtcat tgcccgccag cttgaggagg cgggggcggc gacggtgatg ccgctggcct
     8161 caccgatcgg ttccggacag ggcatgcagg actggggcag catccagcgc atcaaggagg
     8221 ccgtctcggt accggtcgtc gtcgatgccg ggatcggcgc gccctcggat gcggcgatcg
     8281 cgatggagct cggcgccgac gcagtgctgg tcaacacggc ggtcgcgaag gcgaccaacc
     8341 cgccggtgat ggccgaggcg atgaagcacg gcacgatcgc cgggcggctc ggctacctgg
     8401 cgggccgcat gccgcgccag cagcacgcgc agccctccag cccgacgggg ggcgtgccga
     8461 ccgcgctcgc cagtgccacc aacggccggc ggtaagcgtc attccgtacg ggagggttgc
     8521 caaagacatt ggcccttgcc ctcgaacctg tcaggtcacg aagccataga ctgggaaagc
     8581 cctcgtcggt ccgagcttgc cgagaagtga cactgcgctg ggatgcgctt gattcctggg
     8641 cctcgccgga gcattcgatt cctcctgcta catcccaccg attcgagtag gctgacacca
     8701 gcgccgtggg tagcggcgcg gtggatcgct tcggaaagcg gcgcgacagc ttgaccgtcg
     8761 actcggacaa ttcgctggca tccgcacgcg acgactggca gtgcgcggga tggcaggttg
     8821 ggggcgggta cttctggtac tacgccggca tcggtgcctt catccccttc gtcgcgctcc
     8881 attttcgcga gctcggctac agcgggccgc aggttgggct gctgacagcg ctgccctcgg
     8941 ttggggtcgc gatcggcggg ccgctggccg gggcggtcgc cgatgccctc agcgtgcatc
     9001 gctgggtact gcggatcgcg ctgctggtcg gtacggtgct ggcactgctc gcctcgatga
     9061 tcgaggcgtt tccgttcatg ctcgccgcga tcgcgccgct tgcgctcgtg ctctcacccg
     9121 tcccgtcgat gatggacagc tatgccgtga cggccagcga gcgagcgaat cgttcctacg
     9181 gcgcgctgcg ggtgtggggc tcggtcggct acatggcggc agtcctcgtc atgggccgcg
     9241 tgatgggtga acgggtctcg gcgctcctct tcgtgggcta cgcgctctgc ctgggactga
     9301 cgctgctgac ggtcttcgcc ctgccgccac tggcggagcg acgcgcgcag ccgctgctga
     9361 ccggtatcca cgcggcgacc gccaaccggc cgctgatgac gctgctgctg gtcgcctacc
     9421 tgctttcgat cggcacagcg gccatgaaca tctacctcgg cgtccatctg gaggagatcg
     9481 gcggctcggc gagcctgatc ggcgtcgcct tcgcgatcag cgcggccagc gagttgccga
     9541 tcgtcgcctt cggcagctgg ttcctggtcc ggctcggtcc ggtgcggctc gcggcgctcg
     9601 cgatcgtcgt ctacgcgctc cgcttcgccg ggtacagcgc gatcacagtg ccggagtggg
     9661 tgcttggcat ccagctcttc cacggactct cgtacggcgc gtttctgatc gcgtccgtca
     9721 cgctcgcaca ccggctcgca gggcaggagc aggcgtcgac cgcgcaggcg ctgctgacgg
     9781 cgatgtcgtt cggcttcggc agcatcaccg gctcactgct cggcggcgtc ttgctcgatg
     9841 tagtcggtac ggcggggctc ttcgggggat cggcgggact gatggtcgtc accctcgccg
     9901 tgctagcgat cgggaatcgt ctcgtcgggc ttggtggcga caaggtggca ggggcgcacg
     9961 cgagtggcta acccggcacg agtgagcgcc gtctgggacc gggccgacct caaggtcgcg
    10021 ggcgggtact tcgcctactt cgcagccgtt ggtgcgctga tgccgtacgt cgcgctctac
    10081 ttccggggca tggggttcag cggactgcag gttggcgtgc tcaccgcgct gccgtctgtc
    10141 ggcctcgccg tgttcgctcc cttgtggggt gccgttgccg acgccatggg cgtccaccga
    10201 ttgctcatgc gcggggcgct tgggctcgcc atcgctgtcg cgttcctcac gacgcaggcg
    10261 tcgactttca tgtcaatgct cctcctgttc ggcctgctcg cgttcgtctc cgtaccggtc
    10321 gccccgttga tggacagcta cggcgtgacc gcgagcgagc acacccgacg gtcgtacggc
    10381 gggatccgcg tgtggggctc gatcggctat atggtgtccg tcctgatcgt gggctggctc
    10441 atgggtgacg agacgtcgtc acttctgctc gttgcgcatg ccgcgtt
//
LOCUS       DASYWL010000110         1535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_361507, whole genome shotgun sequence.
ACCESSION   DASYWL010000110 DASYWL010000000
VERSION     DASYWL010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361507"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(295..1491)
                     /locus_tag="VGR22_04075"
     CDS             complement(295..1491)
                     /locus_tag="VGR22_04075"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEV2127778.1"
                     /translation="MSIVLVVNSGSSSIKYQLIEMENETVLAKGLIERIGTPRGIATH
                     TDPSGEQHVLERPVADHIAGFELMIDACDDYGPNLSTVPIVTVGHRVVQGGARFIVPT
                     VIDDEVVTTIEEISPLAPLHNPPNLLGIQAARQAFPDIQHVAVFDTAFHQTLPEEART
                     YAIDADLAQQYQIRRYGFHGTSHKYVSERAAELLGRPLEAVNSIILHIGNGVSATAVQ
                     GGRSVETSMGLTPLEGMVMGTRSGDIDPGILFHLHRESGMTVEQLEDLLNQRSGLQGL
                     TGTADMRDVLAAVESGDPQAKLAFGVYCHRIKSYIGAYYAHLGRVDAIVFTAGVGENV
                     PEVRARSLAGLEHMGIAVDPDRNASGEREPRRISSDDSDITVLVIPTNEELEIARQTL
                     AVVGRE"
BASE COUNT          243 a          501 c          501 g          290 t
ORIGIN      
        1 ggccggtctg cgtgcctttt cagccgccga tccgggccct tcaccgggaa aaggccggca
       61 caagcgctgg acactccccg gctccatgtg ggatgacgag aacgatgcca gtcagagcgc
      121 cccgaacgtc gttcgacaca gtgcagccca cccgcagagg ggcgtgtcca tttgatagat
      181 cagctatctc tcgggaccgc cgagtgtgtc cctgtctacc tgccaatgta ccctcgcacg
      241 cgaactgaca cgcaccacgt cagttcgcga taccacgccc ttcgcccctc gcggctactc
      301 gcgtccgacc accgcgagcg tctggcgggc gatctcgagt tcctcgttcg tggggatgac
      361 cagcaccgtg atgtcagagt cgtctgacga gatgcggcgg ggttctcgct ccccggaggc
      421 attccgatcg gggtcgaccg cgatgcccat gtgctcgagc ccggcgagcg accgcgctcg
      481 cacctccggc acgttctcgc cgacgcccgc cgtgaagacg attgcgtcga cccgaccgag
      541 atgcgcgtag tacgcgccga tgtagctctt gatccggtgg cagtagactc cgaaggccag
      601 cttcgcctgc ggatcgccac tctcgacggc agcgagcacg tctcgcatgt cggccgtgcc
      661 ggtcaagccc tgcaggccgc tgcgctggtt gagcaggtcc tcgagttgct ccacggtcat
      721 gccgctctcc cgatgcaggt ggaagaggat accggggtcg atgtcaccgc tgcgggtgcc
      781 catcaccatt ccttccagcg gcgtcaggcc catcgacgtt tcgacggacc tgccaccctg
      841 cacggctgtt gccgacacgc cgttgccgat gtgcaggatg atcgaattga ccgcctccag
      901 cggccggccg aggagttccg ccgctcgctc ggagacgtac ttgtgtgacg ttccgtggaa
      961 gccgtagcgt cgaatctggt actgctgcgc gaggtcggcg tcgatcgcgt aggtccgcgc
     1021 ctcctcgggc agcgtctggt ggaaggcggt atcgaagacg gcaacgtgct ggatgtccgg
     1081 gaaggcctgg cgggctgcct ggatgccaag caggttcggc gggttgtgga gcggcgccag
     1141 cgggctgatc tcctcgatcg ttgtcaccac ctcatcgtcg atgactgtcg gcacgatgaa
     1201 gcgggcgccg ccctgcacca ctcggtgacc gacggtgacg atgggaacgg tggagagatt
     1261 cgggccgtaa tcgtcgcagg cgtcgatcat cagctcgaac ccggcgatgt ggtcggccac
     1321 tggccgctcc agcacgtgct gttctccgct tgggtcggtg tgggtcgcga tcccgcgggg
     1381 agtgccaatc cgctcgatga gccccttcgc gaggaccgtt tcgttctcca tctcgatcag
     1441 ctggtacttg atcgaggagg agccggagtt gacgacgagg acgatgctca tgcggctcca
     1501 tcctctccct gtgcttggat gacggtaatg gcaac
//
LOCUS       DASYWL010000111         4476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_305353, whole genome shotgun sequence.
ACCESSION   DASYWL010000111 DASYWL010000000
VERSION     DASYWL010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305353"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VGR22_04080"
     CDS             complement(<1..185)
                     /locus_tag="VGR22_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127779.1"
                     /translation="MFRWFACTVVRHKWLVAVFWIALVLVSVPFALDAEDPLEVGGFS
                     SDETEASRTIRAVQDRLG"
     gene            complement(223..525)
                     /locus_tag="VGR22_04085"
     CDS             complement(223..525)
                     /locus_tag="VGR22_04085"
                     /inference="COORDINATES: protein motif:HMM:NF017880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HEV2127780.1"
                     /translation="MARARRGAAYLPESFDANGFIHCTNGFESLCNVGNMFCTADSRD
                     YLVLALDVTKISSEVRYDHPERIYPHIYRPINAEAVIGLMDVERNADGTFIGFTER"
     gene            705..1769
                     /gene="selD"
                     /locus_tag="VGR22_04090"
     CDS             705..1769
                     /gene="selD"
                     /locus_tag="VGR22_04090"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:756..758,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEV2127781.1"
                     /translation="MHDREQIRLTTLANCAGUASKLDAGALAQVLQPLTIATHPDLIV
                     GLQTSDDAAVLRLSDEVALVQTIDFFAPVVDDPYEFGAIAAANSMSDVFAMGGTVTMG
                     LNVAAFPEGLDLAILSEIFRGGADKMTEAGGVIAGGHTVTDDEPKYGLVVTGMVHPDH
                     LWTKAGAQPGDVLFLTKPIGSGLLTTAAKQERIGRGELQPAIELMMRLNLYARNIAVK
                     HSVHAATDITGYALAGHAWEVAQRSRVALRFALNALPLIDGAADLADAGVGAGGLHSN
                     RTHFTGIANGVRVEAGPEDPLQSLVFDPQTSGGLLFAVPASAAEEFESAFYRSGEPLW
                     RVGDVTDGSGVVFTAEQLLR"
     gene            1766..2611
                     /gene="rsmA"
                     /locus_tag="VGR22_04095"
     CDS             1766..2611
                     /gene="rsmA"
                     /locus_tag="VGR22_04095"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEV2127782.1"
                     /translation="MSEPGGREDWVRRMRQWGVRPSRSKGQNFLLDDEVVSGIAEAAR
                     AGDGVPLLEIGPGLGILTEELLARGADVTAIELDDTLASRLRDHFGDHPRFRLIDGDA
                     TVVDPTELFARDKPYRIVANLPYSVATVIIRHFLESGHPPEELVVMVQREVAERMTAS
                     AGDLSLLALAILLYTVPGYLFTVPKEAFHPVPKVTSAVVRLRVRAEQLLTARERDRLF
                     ELATVAFQQKRKTLSNSLAPEVGMDKPDFVKRLAAIGIDASLRPQALAFEDWLRLARL
                     EVAEA"
     gene            2608..3483
                     /gene="ispE"
                     /locus_tag="VGR22_04100"
     CDS             2608..3483
                     /gene="ispE"
                     /locus_tag="VGR22_04100"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEV2127783.1"
                     /translation="MSVRKIDAPAKINLGLEILGKRRDGYHEVRTVLAAISLFDELVF
                     DSASESYDSLRLEPDTEDIPSEANLVTKALSAMREAGADIPPQRISIVKRIPAAAGFG
                     GASSDAAATLRAFSPELATAEIDAADLAVSLGSDVPFFLNGPVALAAGRGEALSPLPR
                     SARDVWVVLAMPDLEIPEKTRTMYSAIDPAWWSDGSRAGRIAENLPTVPTSAPFNVFE
                     RALLRIHPELEIWRRGLREAGFPFVALTGAGPTHYTLALSQEQARDIQAQLSSIGVQA
                     ITACLLSRWEGADDQ"
     gene            3473..3604
                     /locus_tag="VGR22_04105"
     CDS             3473..3604
                     /locus_tag="VGR22_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127784.1"
                     /translation="MTSDGSARLSRRQVIVSAVGGALLGSAWGLALGEASSDDRRPV"
     gene            3820..4230
                     /locus_tag="VGR22_04110"
     CDS             3820..4230
                     /locus_tag="VGR22_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127785.1"
                     /translation="MATDPDTAYPQIGFADRQIKIGDLAIVGRRLAHGSHTVRGVVSR
                     GPLRGRLITIVPEASALPLLTAIHKAAAVIIVLDEDLDTLPPTPSGITIAAPADAAVF
                     DEMDRDGDRPIRLFRDAPVSFAIPGSGITLPGAG"
     gene            complement(4237..>4476)
                     /locus_tag="VGR22_04115"
     CDS             complement(4237..>4476)
                     /locus_tag="VGR22_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127786.1"
                     /translation="ANHSSVPVRVQQSRRWDGRAGDMPATADRRWSNTWPSDAPGIRQ
                     EERASRQSEVQQARERHRSHHRGGSGRAEENGGSR"
BASE COUNT          785 a         1455 c         1417 g          819 t
ORIGIN      
        1 cccaaccggt cttgcacggc acggatcgtt cggctggcct cggtctcgtc agatgagaat
       61 ccgccaacct cgagcggatc ctcggcgtcg agggcgaacg gcaccgacac gagcacgagc
      121 gcgatccaga agactgctac cagccacttg tgccgaacga ccgtgcatgc gaaccatcta
      181 aacatgagcg gcgctcattc ctgtcccctg caccttccgg ccctaccgct cagtgaatcc
      241 gatgaaggtg ccgtcggcat tccgctcgac gtccatcagg ccaatcacgg cttcggcgtt
      301 gatcggtctg tagatgtgcg gatagattcg ctcgggatgg tcataccgca cctcggagga
      361 gattttcgtg acgtcgagcg ccagcacgag gtagtcgcga ctatcagccg tgcagaacat
      421 gttgcccacg ttgcagagcg attcgaaccc attggtgcag tggataaagc cgttggcatc
      481 aaaggactcc ggcaaatacg cggccccccg ccgcgctcga gccacagcgg ggccggaacg
      541 agatggtagg tgacgccggc ttgatggctt gcgtcggcgg tctggtaaat catcggcttc
      601 ctcgcgtcgg tgcgtagggg ttggacgccc ggggtgtcgt ggtctatcgt ccaatcgtac
      661 gcgcccggtg tgggcgacgt gtcaggaact gtacaggaag accaatgcac gatcgtgagc
      721 agattcgact gaccacactc gccaattgcg ctggctgagc atcgaagctc gacgcgggcg
      781 cattggcgca ggttctgcag ccactcacca tcgcgaccca tccggacctc atcgtcggac
      841 tccagacctc cgatgatgca gccgtgctgc gcctgagcga cgaagtcgcg ctcgtgcaga
      901 ccatcgattt ctttgcgccc gtggttgacg acccgtacga gttcggggcg attgctgctg
      961 ccaacagcat gagcgacgtg ttcgcaatgg gtggaacagt gacgatgggc ctcaacgtcg
     1021 cggctttccc ggaaggcctc gacctcgcga tcctctccga gattttccgg ggcggcgctg
     1081 acaagatgac ggaagcgggc ggtgtgatcg ccggcgggca taccgtcacg gacgatgagc
     1141 cgaagtacgg gctcgtggtg accggcatgg ttcatcccga tcacctctgg acaaaagcag
     1201 gcgcgcagcc cggcgacgtc ctctttctca ccaagccgat tggctccggc ctgctgacca
     1261 ctgcagcgaa gcaggagcgc atcggccgag gcgaactcca gcctgccatc gagctgatga
     1321 tgcggctcaa cctgtatgcc cggaatatcg ccgtcaagca ttctgtacac gcggcgactg
     1381 acatcaccgg ctacgccctc gccggtcatg cctgggaggt tgcacagcga agccgtgtcg
     1441 cgctgcgctt cgcgctgaac gcgttgccgc tgatcgacgg cgccgccgac ctcgccgatg
     1501 cgggggtcgg cgctggcggg ctccatagca accgcacgca cttcaccggt atcgccaatg
     1561 gcgtgcgggt ggaagcgggg ccagaggatc ccttgcaatc cctcgtgttc gacccgcaga
     1621 cctcgggagg attgctcttc gccgtgcccg cctctgccgc cgaggagttt gagagcgcct
     1681 tttatcggtc gggggagccg ctttggcggg tcggcgacgt aaccgacgga tccggcgtcg
     1741 tctttaccgc cgagcagctc ctgcgatgag cgaacccgga ggacgagagg actgggtccg
     1801 gcgaatgcgt caatggggcg tgcggccgtc ccgatcgaag ggtcagaatt tcctgcttga
     1861 tgacgaggtg gtctccggga tagcagaggc agcgcgagct ggcgatggtg tcccgttact
     1921 ggaaatcggt cccggtctgg gcattcttac cgaggagctg cttgcccgcg gggcggacgt
     1981 gacggccatc gagctcgacg acaccctcgc gtcccgtctt cgggaccact tcggggacca
     2041 cccgcgattc cgactcatcg atggggacgc tacagtcgtc gatccgaccg aactcttcgc
     2101 tcgcgacaag ccctaccgca tcgtcgccaa cctcccctac agtgtcgcca cggtcatcat
     2161 ccggcacttc cttgagtcgg gacatcctcc cgaggagctc gtggtgatgg tgcagcggga
     2221 ggtcgccgag cgcatgacag ccagcgccgg ggatctctca ctcctggcgc tggcaatcct
     2281 gctctacacc gtgcctgggt acctctttac cgtcccgaag gaggcgttcc acccggtacc
     2341 caaggtcacc tcggcggtgg tccggctgcg ggttcgtgcg gagcagctcc tcaccgcgcg
     2401 cgagcgagac cgactcttcg agctggcgac cgtcgccttt cagcagaagc ggaaaaccct
     2461 gtcgaactcg ctcgcacccg aggtcggcat ggacaagcct gacttcgtga agcgccttgc
     2521 ggcaatcgga atcgacgcct cgctgcggcc gcaggcactc gccttcgaag attggctgcg
     2581 tctggctcgg cttgaggtcg ccgaagcatg agtgtacgaa agattgatgc gccggccaag
     2641 atcaatctgg gtctggagat cctcgggaaa cgacgcgacg gctaccatga ggtgcgaacc
     2701 gtcctggctg caatctcgct cttcgacgag ctggttttcg attcggcgtc cgaatcatat
     2761 gactccctgc gcttggagcc tgataccgag gatatcccga gtgaggcgaa tctggtcacg
     2821 aaggcgctat cagcgatgcg agaagcaggc gctgacatcc cgccccagag gatctcgatc
     2881 gtgaagcgga ttcccgctgc tgctggcttc ggaggtgcaa gcagcgacgc cgccgcgaca
     2941 cttcgcgcct tctcgcccga actcgccacc gccgagatcg atgcagcgga tctcgccgtc
     3001 agcctcggca gcgacgtgcc gttttttctc aacggtcctg tcgcgctcgc ggccggtcgt
     3061 ggagaggcgc tctcaccgct gccccgatca gccagggatg tctgggtagt gctggcgatg
     3121 ccggacctcg aaatccctga aaagacccgt acgatgtaca gcgctattga tccagcatgg
     3181 tggtcggacg gcagcagggc ggggcgcatt gcggagaatc tcccaaccgt acccacctcc
     3241 gcgccattca acgtcttcga gcgagcactc ctccgaatcc atccggagct cgagatctgg
     3301 cgaagaggcc tgcgggaggc cggctttccg ttcgtggcgc tgaccggcgc gggaccgacc
     3361 cactacaccc ttgctcttag ccaagagcaa gctcgggaca tccaggccca gctttcctcg
     3421 atcggtgtgc aggcgatcac ggcttgtctt ctgtcgcgct gggagggcgc cgatgaccag
     3481 tgacggttca gcacgactca gccgccggca ggtcatcgtg tcggcagtcg gcggcgccct
     3541 gctcggctcc gcatgggggc tggcactcgg cgaggcatca agtgacgacc ggcggccggt
     3601 gtaaccatcg tctggaaccg ctcgggattc gccgcactcg tcgagcatca gctccagcgt
     3661 gtcctgctgc tccattccga tgatgccaag cggcagcgcg aactcaccca actggtcacc
     3721 ggattcacgc gacagcgcgt cgatgtcctg ctcgctcccg atgcgacgct ccaagcgctc
     3781 tcgacggaca tgaaggagcg ctggaggctg gcgtaggtca tggccacgga tccggatacc
     3841 gcatatcctc aaatcggctt tgccgatcgc cagatcaaga tcggcgactt ggcgattgtt
     3901 ggccggcgcc tggcgcacgg cagccacacc gttcgaggag tggtatctcg aggtccgcta
     3961 cggggacggt tgatcaccat cgtgccggag gccagcgcgc tgccgctgct cacagcaatc
     4021 cacaaagcgg cagcggtcat catcgtgttg gatgaggatc tggacacact cccaccgaca
     4081 ccgtcaggca tcaccatcgc agcgccagcg gacgccgcag ttttcgacga aatggacagg
     4141 gacggtgacc gcccgattcg gctcttccgc gacgcaccgg taagcttcgc gatccccggt
     4201 tccggaatca cgctacctgg cgctggatga ggtcgctcag cggctgccac cgttttcctc
     4261 ggcgcgccca gaccctcccc ggtgatgtga gcggtgacgt tcccgagcct gctgcacctc
     4321 gctttggcga gacgcccgct cctcctgtcg gatgccgggc gcgtcgcttg gccaagtgtt
     4381 ggaccaccga cgatctgcgg tcgcgggcat gtctcccgcc cgaccgtccc aacggcgcga
     4441 ttgctgcacg cggaccggca ccgaggagtg gttggc
//
LOCUS       DASYWL010000112         3755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_605811, whole genome shotgun sequence.
ACCESSION   DASYWL010000112 DASYWL010000000
VERSION     DASYWL010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605811"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(411..1796)
                     /locus_tag="VGR22_04120"
     CDS             complement(411..1796)
                     /locus_tag="VGR22_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HEV2127787.1"
                     /translation="MAAETTKYALSEGRIPTAWYNITADLPVAPVPPLHPGTKQPIGP
                     DDLAPLFPMALIAQEVATDRYVQIPDPVRDVYRLWRPTPLYRAHRLEKALDLPAGVRM
                     LYKYEGTSPAGSHKPNTAVPQAYYNAEAGVKRITTETGAGQWGSSLAMACAFYGIELK
                     VYMVKVSYEQKPYRRALMETWGATCVPSPSTDTNAGRQILEETPDSTGSLGIAISEAV
                     EDAASREDTRYSLGSVLNHVLMHQTVIGEEALEQLDIADAWPDVVIGCVGGGSNFAGL
                     TFPFVRENLTNGKNVRILAVEPSACPTLTRGVYAYDFGDTAGLTPLVKMHTLGHTFVP
                     AGIHAGGLRYHGSAPLVSLLLDQGLIEATAVNQLAAFEAAVQFSRTEGIVPAPESAHA
                     VQQAVAEALTAKEAGEDKTILFGLSGHGNFDLAAYQNYLAGKLTDYEHPQEAIDEALQ
                     HLPDVSGVAAD"
     gene            1982..2755
                     /locus_tag="VGR22_04125"
     CDS             1982..2755
                     /locus_tag="VGR22_04125"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2127788.1"
                     /translation="MNYTLVRHATAVFEIGGRRFLIDPMLDPAHARPAIRNTPNQQQN
                     PLVDLPDGWEQLIASPDALVVTHLHADHFDGTAERTLDHDLPLLCQSEDAERLRQKGF
                     SDVRGIEHEAELDGVTLMRTIAQHGTGAIGLAMAPASGFVFLAQGEPRVYVAGDTIWC
                     EEMSEMIATHLPEVIVLNASGARFLEGDPIVMTAEDVAKVHKAAPDALLIVVHLEAIN
                     HCLETRQHYRETLPELGVALGRVRIPEDGETVTWEPRSS"
     gene            3187..>3755
                     /locus_tag="VGR22_04130"
     CDS             3187..>3755
                     /locus_tag="VGR22_04130"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEV2127789.1"
                     /translation="MASLSARYRIGIGGIAIESSTFSPLHSTTDDFRIRRGSEMQEMY
                     PYLPNWTCRDRDDIEFVPCLKARALPGGQVTADAYATMKGELLDRVRQVLPLDGFFYD
                     VHGAMSVVGMDDAEGDLASAIREIVGPDCVISAGMDLHGNVSDRLVSQIDVFTCYREA
                     PHIDAMETKERAVAHLLRCLDTGTRPHRA"
BASE COUNT          649 a         1183 c         1258 g          665 t
ORIGIN      
        1 gtacctcgag cgggcgaagc agctcgatgc caagattcag ctcttcaaca actccgttcc
       61 gcgcaatctc tggcatctgg agaaactgcg cttcctgccc gccaaggcct cgcagcgctt
      121 cgacaccgcg gttccgaagc gatgacctga tgctggctgg aagcaatcgt tgctcaactc
      181 aacgtgtaga ccgggactga catgtcgcat tgtcctgtaa gatcttggtc tcaccggggc
      241 cggctcactt ggtggcagca taggtgcttc gaccggatcg tcagataagc cgtgcggcct
      301 actggccgac gtccattcga acccgccatt tgaggcaccg ggctgcgagt ggatgcaacc
      361 aagaaggccc gagtggagga acccacccgg acctttatac gcgatcacgg ctagtccgca
      421 gcgacgccgg agacgtcggg gaggtgctgg agtgcctcgt cgatcgcctc ctgaggatgt
      481 tcgtagtcgg tcagcttgcc agccaggtag ttctggtagg cggcgagatc gaagttgccg
      541 tggccggaga gcccgaagag gatcgtcttg tcctcgccgg cctccttcgc ggtaagcgct
      601 tcagcgaccg cctgctggac ggcgtgggcc gactccggtg ccgggacaat gccctcagtg
      661 cgggagaact gcaccgcggc ctcgaacgcg gcgagctggt tgaccgccgt cgcctcgatg
      721 agtccctggt ccagcagcag gctcaccagc ggcgcggagc cgtggtagcg caagccaccg
      781 gcgtggatcc cagccggcac gaaggtgtgc cccagcgtgt gcatcttgac cagcggggtc
      841 aagccggcag tgtcgccgaa atcgtaggcg taaacgccac gcgtgagcgt cgggcaggcc
      901 gacggctcga cggcgaggat gcgaacgttc ttcccgttgg tgaggttctc ccggacgaag
      961 gggaaggtaa gcccagcgaa attggagccg ccgccgacgc agccgattac gacatccggc
     1021 caggcatcag cgatgtcgag ctgctccaat gcctcctcgc cgatcaccgt ctggtgcatc
     1081 agcacatggt tgagcaccga tccgagcgag tacctggtgt cctcgcggct ggcggcgtcc
     1141 tcgaccgctt cggagatggc gatgccgagc gagccggtgg aatccggcgt ctcctcgagg
     1201 atctggcggc cagcgttggt gtccgtgctc gggctaggca cgcaggtcgc gccccacgtc
     1261 tccatgagcg cgcggcggta cggcttctgc tcgtacgaca ccttgaccat atagaccttg
     1321 agctcgatgc cgtagaaggc gcacgccatc gccagcgagc tgccccactg gccggcaccg
     1381 gtctcggtcg tgatgcgctt cacgcccgcc tcggcgttgt agtacgcctg tggaacggcg
     1441 gtgttcggct tgtgggagcc ggccgggctg gtgccctcgt acttgtagag catcctgacg
     1501 ccggcgggca gatcgagcgc cttctcgagc cggtgtgctc ggtagagcgg ggtcgggcgc
     1561 cagaggcgat agacatcgcg gaccgggtcc ggaatctgga cgtaccggtc cgtcgccacc
     1621 tcctgtgcga tcagtgccat cgggaaaagc ggcgcgaggt cgtccgggcc gatcggctgc
     1681 ttggtgccgg ggtgcagcgg tggtacgggc gcgaccggga gatcggctgt gatgttgtac
     1741 caggctgtcg ggatacgacc ctcactcagc gcatacttcg tcgtttcggc cgccatgtcc
     1801 taacctttcc tggcgggaga gtcccacgtg tgttcagacg cgattgtcct gaggtccatt
     1861 gtatccggca ggcgccgcac gaggggcagg atgtgatgcc catgccacac gaacgagcca
     1921 atgtcgcgga cgtgctaccc tcggtccaca ccgaccggat cgaagcagga gggaggcgac
     1981 catgaactac accctcgtcc gacacgcgac cgctgtgttc gagatcggcg gtcgccggtt
     2041 cctgatcgac ccgatgctgg acccggccca cgcccggccg gccattagga acaccccgaa
     2101 tcagcagcag aatccgctcg tcgacctgcc cgacgggtgg gagcagctga ttgcctcacc
     2161 cgacgcgctg gtggtcacgc acctgcatgc ggatcacttc gacggcacgg ccgagcggac
     2221 cctcgaccac gacctgccgc tgttgtgtca atcggaggac gccgagcggc tgcgccagaa
     2281 ggggttcagc gacgttcgtg ggatcgagca cgaagcggaa ctcgatggcg tgacgttgat
     2341 gcggaccatc gcgcagcatg ggaccggcgc gatcggcctg gcgatggcgc cggcgtccgg
     2401 gtttgtcttc ctggcacagg gagagccccg ggtctacgtg gcgggtgaca cgatctggtg
     2461 cgaggagatg tcggagatga tcgccacgca cctgccggag gtgatcgtgc tcaacgcgag
     2521 cggcgcgcgg ttcctggagg gcgaccccat cgtgatgacg gcggaggacg tcgcgaaggt
     2581 ccacaaggcg gcaccggatg ccctgctgat cgtggtgcat ctggaggcga ttaaccattg
     2641 cctggagacg cgacagcact accgggagac gctgcccgag ctcggcgtcg ccctcggccg
     2701 ggtccgaatc cccgaggacg gcgagacggt gacgtgggag ccacgatcat cgtaggggaa
     2761 accgctacgt gtcgtttcga ccgaggcacg cgaagcgtgc ggagtggaga gaatctctcg
     2821 cgaaggtgag gacaggatgc gatcgttgac gatcgctgca caagagattt ctcgacttcg
     2881 cggctgcgtc gattcgctcg aaatgacagc ttgagtggtc ctccgccgtt ttccgcatgt
     2941 caccctgcgc ggtaccagtt actgaggagc gggttgagct cccgctgcat cgcccagacg
     3001 tcgcagtcct gattcgcgag cagcacgcag gcgatgtccc gacccgggaa ccacgtcgcg
     3061 agcttcgcga ccccggcgac acaccactca ttgccttacc cttacccgtt gtgccctgcc
     3121 cgcttctcgg atagggtact ccgcggtagt tgagacgccc atcgtttgtg aggagcaggg
     3181 tctgccgtgg catcactttc agcgcgctat cgaatcggga tcggcggcat cgccatcgag
     3241 agcagcactt tctcgccact gcacagcacg actgatgact tccgcattcg acgcggttcc
     3301 gagatgcagg agatgtaccc ctacctgccg aactggacgt gtcgcgatcg cgacgatatc
     3361 gaattcgtcc catgtctcaa ggcacgagcg ctccccggcg gacaggtaac cgccgacgcc
     3421 tacgccacga tgaagggtga gctgttggat cgggtccgcc aggtcctgcc gctcgacggc
     3481 ttcttctacg atgtccacgg cgcgatgagc gtggtcggca tggacgatgc cgagggcgat
     3541 ctcgcgtccg cgattcggga gatcgtcggg ccggattgcg tcatctcggc tggcatggac
     3601 ctgcacggga atgtcagcga ccggctcgtc tcgcagatcg acgtctttac gtgctaccga
     3661 gaggcgccgc acatcgacgc gatggagacg aaggagcgcg ctgtggcgca cctgctgcgc
     3721 tgcctggaca ccggcacgcg gccgcaccgc gcctt
//
LOCUS       DASYWL010000113         1850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_549371, whole genome shotgun sequence.
ACCESSION   DASYWL010000113 DASYWL010000000
VERSION     DASYWL010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549371"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..612
                     /locus_tag="VGR22_04135"
     CDS             22..612
                     /locus_tag="VGR22_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127790.1"
                     /translation="MSRLFYRLVEGNPPSLRDVMSYKALGRPLLNEANREVWAGGVSV
                     QNTMAQAHNRAKVLRGKTHVAVLEIPDGANVRWERTGEKRGHVTLWGDPQELLSYVRD
                     VIGLERSYRESTVKPCFELWDSDSANFLGAYESIPDALADLDRTFPTAQLKQQVMDLV
                     LTLELGGDDDDTMILLDGASLYRLLRPIPASELKAS"
     gene            652..1605
                     /locus_tag="VGR22_04140"
     CDS             652..1605
                     /locus_tag="VGR22_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2127791.1"
                     /translation="MLGFLIRRLATVVVPTLLGISILVFGAMHMIPGSFVDVAIGIGP
                     EVSEAQREVIAERYGLDAPLPVQYLRWLGNLLQGDLGDSLRTGDPVVEEIVGRLPATL
                     ELTLLATVVSLVIALPAGIVAAVNRDGPWDVMLRVIGLLGLSVPNFLIATFLVLFVST
                     RWPVLPTTGFVPLGEDIAGNLKSLALPAISLGALLAASAMRMMRSSVLEELGKDYLMV
                     ARAKGLPDRAVVMRHAVRNALVPVITVVGIQTGYLLGGTVIIEQIFAIPGIGRLALDA
                     VLQRDYPVVQGTTLFIAAAFVAMNTLADVVYGLVDPRIRVR"
     gene            1605..>1850
                     /locus_tag="VGR22_04145"
     CDS             1605..>1850
                     /locus_tag="VGR22_04145"
                     /inference="COORDINATES: protein motif:HMM:NF024313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2127792.1"
                     /translation="MATERPSRDPRRVISALLRHHLTGAIAGLLLVLIVLAALLAPWL
                     ATGDPTDMQPIERLRSPGEAGPMGTDVFGRDIWSRILY"
BASE COUNT          286 a          580 c          631 g          353 t
ORIGIN      
        1 gcatagtgag cagaatgtcg catgtctcgc ttgttctatc gtctggtcga aggcaatccg
       61 ccctctctcc gggatgtcat gtcgtacaag gcgctcggca ggcccctgtt gaatgaagcg
      121 aatcgagagg tttgggcagg aggagtgtcg gttcagaaca cgatggcgca agcgcacaat
      181 cgggccaaag tgctgcgagg caagacccat gtggccgttc tcgagattcc ggatggcgcg
      241 aatgtccggt gggagcggac gggggagaag cgtgggcacg tcacgctctg gggcgatcca
      301 caagaactgc taagctatgt ccgcgatgtg atcgggctgg aacgatcgta cagggagtcc
      361 accgtgaaac cgtgcttcga actctgggac tcggacagcg cgaatttcct gggcgcctat
      421 gaatccatcc cggacgcatt ggctgatctc gacaggacct tcccgactgc tcaattgaaa
      481 cagcaggtta tggacctcgt gctcacgctc gaacttggtg gggatgatga cgacaccatg
      541 atccttctcg atggagcgtc actgtaccgc ctcctccggc cgatccctgc gtcggagctc
      601 aaggcttcct gagttgggct ggcattgctt gtatcgaact cgtagagatg catgctcggc
      661 ttcctgatcc ggcgcctggc caccgtggtc gtgccgacct tgctcggcat ctccatcctg
      721 gtgttcggcg ccatgcacat gatccccggc agcttcgtcg atgtcgccat cggcatcggc
      781 ccggaggtga gcgaggcgca gcgcgaggtc atcgccgagc gctacgggct cgacgcgccg
      841 ctgccggtgc aataccttcg ctggctcggc aacctgctgc agggcgacct gggcgactcg
      901 ctgcgcaccg gcgatccggt tgtcgaggag atcgtcggcc gcctgccggc cacgctggaa
      961 ctaacgctgc tggcgacggt cgtctcgctc gtcatcgccc tcccggcggg gatcgtcgcg
     1021 gcggtgaacc gcgacggtcc ctgggatgtg atgctgcgcg tgatcggcct gctcggcctc
     1081 tcggtgccga acttcctgat cgccaccttc ctcgtgctgt tcgtctcgac gcgctggccg
     1141 gtgctgccga ccaccggctt cgtgccgctc ggcgaggata tcgcgggcaa cctcaagtcc
     1201 ctggctctgc cggcgatctc gctcggagcg ctgctggccg cgtcggcgat gcggatgatg
     1261 cggtcatcgg tgctggagga gctcggcaag gactacctga tggtcgcccg cgccaaaggg
     1321 cttccggacc gggcggtggt gatgcggcac gccgttcgca acgcgctggt gccggtgatc
     1381 acggtggtcg gcatccagac aggatacctg ctcgggggaa cggtgatcat cgagcagatc
     1441 ttcgccattc ccgggatcgg gcggctggcg ctcgatgcgg tgctgcagcg cgactatccg
     1501 gtggtgcagg gcacgacgct cttcatcgcc gccgcgttcg tggcgatgaa caccctcgcg
     1561 gacgtcgtct acgggctggt tgacccgcgg atccgggtgc gctgatggcg acggaacggc
     1621 cttcccgcga cccgaggcgc gtcatcagcg cgctgctgcg acaccacctg accggcgcga
     1681 tcgctggact cctgctggtg ctgatcgtgc tggcggcgct gctggcgccg tggctggcga
     1741 ccggcgaccc gaccgacatg cagccgatcg agcggctgag atcgccgggc gaggcgggcc
     1801 cgatgggaac cgacgtgttc gggcgggata tctggagccg catcctctac
//
LOCUS       DASYWL010000114         2575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_267845, whole genome shotgun sequence.
ACCESSION   DASYWL010000114 DASYWL010000000
VERSION     DASYWL010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267845"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1591
                     /locus_tag="VGR22_04150"
     CDS             <1..1591
                     /locus_tag="VGR22_04150"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEV2127793.1"
                     /translation="SSIAKGALEVLAAARTHQSANTVVREVSGNGAFWMEPWIEVKRP
                     GEPPVVYGKVSPDDVGAVLTGARDDLAVGVRAPESFNGIPSLADDPFFSNQRQIITRN
                     AGVIEPDSIEDAIGRGAYDGYFKVLFEMTANEAIEEVTTANVRGRGGGGFPAGIKWAS
                     GRKAKARPKFIVCNSHEGEPNVYKDRHLHEADPHRVLEGILIACIVCGAERGYNYIGG
                     EYPLAIRRFRKAVADAERLGLIGRNVVGTGHDVAFRVRTGGGAYICGEASALMYSVMG
                     VKGQPRTKPPRSVEEGLWKRPTVANNTETLANIPDIIVNGGEWYAAIGTENSKGTKLM
                     TVQGPLKRHGVVEVPMGVTLNHLFNDIFGGMREGSEFKGVQTGGVSAGPLTPEHLDIP
                     VDFDSLSDVGGMLGSGGWVIFDQNVCVVDFARYLTAFNRYESCAKCVPCRLGNPALVE
                     ILDRIRFGRGTLDDLTLIKRTSQHVIELSLCGLGQVAPVPLMGMIQWYEEEFRRHIVD
                     GVCPTGTCPIAAHEPVLVAGD"
     gene            complement(1631..>2575)
                     /locus_tag="VGR22_04155"
     CDS             complement(1631..>2575)
                     /locus_tag="VGR22_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV2127794.1"
                     /translation="RLMLQERSSGVVVVLLGASLWGTIGTTYALIQERVAVDDVTLVT
                     LRAAVIAVAWFAVHDRGAFTIRSSDRGPMVVFGLVSVTAFYLALVYAFRYSSVAVGTL
                     LLYLAPALVTLGAALWFNEPLTRVKVGALLLSFTGLLLIVQVYRPSGLGIDWLGIAFG
                     LAAAVTYGSYSLIGKPLMAHYRPATLLVWNLSIGAAGLLVIKLVVSPMSWPSLVESSG
                     IALYTGLMLSVLPVGLYTVGLSRLPSSEASILATVEPVVAMVLATWLLDERLGPGQLI
                     GAACIVGGVVLLTVSGRRLLGRWHAAWSRGAARRTARG"
BASE COUNT          490 a          884 c          794 g          407 t
ORIGIN      
        1 cagctcgatc gccaagggtg cgctcgaggt gctcgccgcc gcccgcacgc accagagcgc
       61 caacactgtt gttcgcgagg tcagcggcaa cggcgctttc tggatggagc cgtggatcga
      121 ggtcaagcga cccggcgaac cgccagtggt ctacggcaag gtctcgccgg acgacgtggg
      181 cgccgtgctg acgggcgccc gtgatgacct cgcggttggc gtgcgcgccc cagagagctt
      241 caatggcatc ccgtcgctcg ccgatgatcc attcttcagc aaccagcggc agatcatcac
      301 ccgcaacgcg ggcgtgatcg aacccgactc catagaggat gcgatcggcc gcggcgccta
      361 cgacgggtac ttcaaggtgc tcttcgagat gacggccaat gaggcgatcg aagaagtgac
      421 cactgccaac gtgcgaggac gtggcggcgg cggcttcccg gcgggcatca agtgggcgtc
      481 cggccgcaag gcgaaggcgc gcccgaagtt catcgtctgc aactcccacg agggcgagcc
      541 gaacgtctac aaggaccgtc acctccacga ggccgacccg catcgcgtac tcgagggcat
      601 cctcatcgct tgtatcgttt gcggcgccga acgtggctac aactacatcg gcggcgagta
      661 cccgcttgcg atccggcgct tccgcaaggc ggttgccgac gccgagcgtc ttggcctgat
      721 tggcaggaac gtggtcggca ccggccacga cgtcgccttc cgggtccgca ccggtggtgg
      781 cgcttacatc tgcggcgagg cttcggcgct gatgtactcc gtcatggggg tgaagggcca
      841 gccgcgcacc aagccgcccc gatccgtcga ggaagggctc tggaagcggc cgactgttgc
      901 caacaacacc gagacgctcg ccaacatccc ggacatcatc gtgaatggcg gcgagtggta
      961 tgccgccatc ggcaccgaga acagcaaggg caccaagctg atgacggtgc aggggcctct
     1021 caagcgccac ggcgtcgtcg aggtaccaat gggagtcacc ctcaaccatc tcttcaatga
     1081 catcttcggc ggaatgcgcg aggggtccga gttcaagggc gtccagaccg gcggtgtctc
     1141 cgccgggccc ctgacgcccg aacatctcga catccctgtc gactttgact cgctctccga
     1201 cgtcggtggc atgctcggat ctggcggctg ggtgatcttt gaccagaatg tctgcgttgt
     1261 cgatttcgct cgctacctca ccgccttcaa ccgctacgag tcatgcgcga agtgtgtgcc
     1321 ctgccgcctc ggcaacccgg cactcgtcga aattctcgat cggattcgct ttggccgggg
     1381 caccctggat gatctcacac tgatcaagcg cacgagccaa cacgtgatcg agctctcgct
     1441 ctgcggtctc ggccaggtcg cgcctgtgcc gttgatgggc atgatccagt ggtacgagga
     1501 ggaattccgg cggcacatcg tcgatggcgt ctgccccacc ggtacctgcc cgatcgccgc
     1561 ccacgagccg gtgctcgtcg ccggcgacta gcgccgctcc tcagctacgt aacgacgaat
     1621 ggctcggggg tcaccctcgg gccgttcgtc gtgccgctcc ccggctccaa gccgcgtgcc
     1681 accgcccgag cagtcgccgc ccagacaccg tgagcagcac cacacctccg acaatgcatg
     1741 cagctccgat gagctggccg gggccgagac gctcatccag cagccaggtc gccagtacca
     1801 tcgcgaccac aggctcgacc gtcgcgagga tgcttgcctc cgacgaaggg agccggctca
     1861 agccaaccgt gtagagcccg accggcagga ccgacagcat cagtccggtg tataacgcaa
     1921 ttccggaact ctcgaccagc gacggccacg acatcggcga gacgaccagc ttgatgacca
     1981 gcagccctgc cgccccgatc gagaggttcc agactagcag cgtcgccggc cgatagtgcg
     2041 ccatcagcgg cttgccgatc aggctgtaac taccgtaggt caccgccgcc gccagcccga
     2101 acgcaatgcc gagccagtcg atgcccaacc cacttggtcg gtatacttgg acgatcaaca
     2161 gcaaccccgt aaacgagagc agcagcgcgc caaccttcac tcgggtgagc ggctcgttga
     2221 accacagtgc cgctcccagc gtcacgagcg ccggcgcaag gtagagcagc agggtcccga
     2281 cggcgacact ggaataccgg aatgcgtaga ctagcgccag atagaatgcc gtgacgctca
     2341 ccagtccgaa gacgaccatc gggcctcggt ccgaggagcg aatcgtgaat gcccctcggt
     2401 cgtgaaccgc gaaccacgca acggcgatca cggccgcgcg cagcgtcacg agtgtcacat
     2461 cgtcgacggc gacccgctcc tgaatcagcg cgtaggtggt gccaatcgtc ccccacaggc
     2521 tcgcaccaag cagtaccacc accacgcctg aactgcgctc ctgcagcatc agtcg
//
LOCUS       DASYWL010000115         4808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_61423, whole genome shotgun sequence.
ACCESSION   DASYWL010000115 DASYWL010000000
VERSION     DASYWL010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61423"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VGR22_04160"
     CDS             <1..221
                     /locus_tag="VGR22_04160"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HEV2127795.1"
                     /translation="LDIDELPGEEDGHFETLGGFVIEQLGHIPEESEYVDHEGYRIEV
                     ADMDGHRIDKLLVSHVADAAATDRADST"
     gene            complement(282..512)
                     /locus_tag="VGR22_04165"
     CDS             complement(282..512)
                     /locus_tag="VGR22_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127796.1"
                     /translation="MAQQKRNAENVTGVSNVTYDLMSVLTNKLEGIAATEVYKQDAQG
                     DQEALKAFEEMEERDRKDVERLRDLVATRLGK"
     gene            complement(791..2311)
                     /locus_tag="VGR22_04170"
     CDS             complement(791..2311)
                     /locus_tag="VGR22_04170"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEV2127797.1"
                     /translation="MSSNRSPSNPSSERPRRRRRSRAWRQWPELVLLPALVAVVQVLA
                     ITPLLYLFFGEAFGLTGGRPVMWPGGIALLGLVGFWSAKAVQRITTDPNLFPIALGVA
                     WLLSAAVWIGLEPAYGLQGLLAEPGSLVGSRGYLIAPLLLSLAVWWHGIRYATVDYLL
                     TAEEVRGSTWRSWLVLIGSLVLSAMLDNEAGQSALSTTPFVVPALMIASVALVAAAEI
                     HAARVQIGHAGGRPPTWSRWGRLVGGAGMVILVIAVLVLLVLTPGAFSAMIAGIVTVL
                     RAVGQLVLWLLYGVFYILYYAFYALSEIFRALFDVEFGPMEPPEQAPGMPGELGPFEQ
                     REDQGPWEYAELVRWLLLGGLVVSVIFLLFRVSRRGQPEEDVPVGDEERSSVFSADLA
                     KAQLRNLFRRGHRGSRLRRLDLESEPSSVRDTWRYLQVLALRQEVGRQETETPQDFVA
                     RLRAVWSGTGGSLNDLVQRYERSRYGDIESERDTTAAREDWADIYRRRRERGSDTS"
     gene            complement(2274..3593)
                     /locus_tag="VGR22_04175"
     CDS             complement(2274..3593)
                     /locus_tag="VGR22_04175"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEV2127798.1"
                     /translation="MAAMDNANSRSTWDRFTAWWNTPNVGIEIHRWMTAAGALVLLGI
                     VFLQAALVLVGVGVALVALALRLWWDFALVATTYTRRFSTRRAFHGDEVTVTLETVNL
                     KPLPVTRLEVAEYVTKNVEIPGRALERAEVPNRWLFRTLFSLGFYERVDYRYTFRTQR
                     RGWYRFGPATLTATDPFGIVRRKRQYEAVDGVLTYPRVVPLTHVIVPARQPYGDFKPP
                     QSLLEDPMRIAGVRQYEAGDSMRRIHWRATARIGELQTRVSEPTATPIAAIFLDTITF
                     SHLWEGQQSDLLELAVVVAASLSAQLLEGRYQVGMYANAPIPEVSRSVRIAPGRRPGQ
                     LAHILERLAMIAPAFGARIEQMMTEEIPRLPWGASMVVVTCRVTPTMQRMLLRLARST
                     GNQRVVLVAIGDRPELLPELRRRVPVYHLGRQERWDELEQITLQPLV"
     gene            complement(3603..4595)
                     /locus_tag="VGR22_04180"
     CDS             complement(3603..4595)
                     /locus_tag="VGR22_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEV2127799.1"
                     /translation="MATDNSEHLAASAIEQIQQTTSRIVSAIEQVIVGKREVIELTLV
                     GVLCEGHVLLEDVPGTGKTMLARALAAALGGSFNRIQFTPDLLPSDITGALVFNPKTT
                     EFTYRPGPIISNVLLADEINRAGPRTQSALLEAMSERQVSVERELVKLPRPFFVIATQ
                     NPVELEGTFPLPEAQLDRFLIRSTIGYPEAEDERRMLRRFRASGPLESVQPVTDPESL
                     MELVGHVQDVHVSPAVEDYLLNLVRGTRNSAHIEVGASPRAILALARASQARAAVQGR
                     GFVTPDDVRELAVPVLAHRVIPTAQADLLGKGQQQLVEEVVRETSVPTEPIDAR"
BASE COUNT          842 a         1687 c         1499 g          780 t
ORIGIN      
        1 tgctcgacat cgacgagctt cccggcgagg aggatgggca cttcgagacg ctgggcggct
       61 tcgtcatcga gcagcttggg cacattcccg aggagagcga gtatgtcgac cacgaagggt
      121 accgcatcga ggtcgccgac atggatggtc atcgcattga caaactgctc gtgagccatg
      181 tcgcggatgc ggctgccacc gatcgggctg acagcacgta gcgaacggcc cccggtgaga
      241 acacccaggg ccgtttcgtc cgccttcttg tcggctggca gctacttgcc gagcctggtg
      301 gcgacgagat cgcggagccg ctcgacatcc ttgcgatcgc gctcctccat ctcctcgaag
      361 gccttcagcg cctcctggtc gccctgcgcg tcctgcttgt acacctcggt cgccgcgatg
      421 ccctcgagct tgttcgtcag cacggacatc aggtcgtagg tgacgttgct aacaccggtt
      481 acgttctcag cgttgcgctt ctgctgggcc atgtgtccct ccttaggcaa tcttgcttca
      541 cgtctggggc gtctcgcccc ttcaggtcat tcttcgcacg tgggatgcca cggcgcgcgc
      601 cgatcgacgt gacttccgat caacaccata tggcgctatg tcaaccttcg taatcgcgtc
      661 cacctgagga tatgccgctg ccgactgcgc ggttctccgg ctcgtctccg tgcaccagat
      721 accccgcctg cgtgcaaccc ttggcgaact tgtccaagaa gggcttctac cgatcttcga
      781 tcgttgaccc tcacgaggtg tccgagccgc gctcccggcg tcgccggtag atgtccgccc
      841 agtcctcccg ggcggccgtg gtatcgcgct cgctttcgat gtccccgtac cggctccgtt
      901 cgtagcgttg caccagatcg ttgagcgagc cgccggtgcc cgaccagacc gcccgcaacc
      961 tcgcgacgaa gtcctgcggc gtttccgtct cctggcgacc gacctcctgc cgcagcgcca
     1021 acacctgcag gtagcgccag gtgtcgcgca cgctgctcgg ctccgattcc aggtcgagcc
     1081 gccgcaaccg cgaccctcga tgcccacgcc ggaagaggtt cctcagctgc gccttcgcca
     1141 ggtcggcgct gaagacactg ctgcgctcct cgtcaccgac tgggacatcc tcctccggct
     1201 gcccgcgtcg cgatacacga aagagcagga agatgaccga caccaccagc ccacccagca
     1261 gcagccagcg gaccaactcc gcgtattccc acggcccttg gtcctcccgc tgctcgaacg
     1321 gtccgagctc gcctggcatg ccgggcgcct gttccggcgg ttccatgggg ccgaactcga
     1381 cgtcgaacag cgcccggaag atctcggaga gcgcgtagaa ggcgtagtac aggatgtaga
     1441 acaccccgta gagcagccag agcaccagct ggccgacggc ccgcaggacc gtcacgatcc
     1501 ccgcgatcat tgccgagaat gcgccgggtg tgagcaccag cagcacgagc acggcgatca
     1561 ccagaatcac catgccggcg ccaccgacca gccgtcccca gcgcgaccac gttggcggcc
     1621 gcccaccggc gtgcccgatc tgcacgcgtg cggcatggat ttccgccgcc gcaaccagcg
     1681 ctaccgacgc gatcattagc gccggcacca caaatggggt ggtagacaac gcagactgcc
     1741 ccgcctcgtt gtccagcatc gcggagagca ccagggaccc gatcaggacg agccacgaac
     1801 gccatgtgct tccgcggacc tcctccgccg tcagcaggta atcgaccgtc gcgtaccgga
     1861 tgccgtgcca ccagacggcg agcgaaagca gcagcggggc gatcaggtat ccccgggagc
     1921 caaccagtga tcctggctct gccagcaggc cttgcagccc ataggcgggc tccagcccga
     1981 tccacacggc agcggacagc agccacgcca cgccgagcgc aatcggaaag agattgggat
     2041 ccgtggtgat acgctggacc gccttcgccg accagaatcc caccaagccg agcagggcga
     2101 tgccacccgg ccacatcacc ggtcggccac cggtcaggcc gaatgcttcc ccaaagaaga
     2161 ggtacagcag cggtgtgatc gcgagcacct gcaccaccgc aaccagcgcg ggcagcagca
     2221 ccagttccgg ccactgccgc catgcccggc tccgtcgacg gcgccgaggc cgttcagacg
     2281 aggggttgga gggtgatctg ttcgagctca tcccatcgct cctgtcttcc caagtggtag
     2341 accggcaccc gccgtcgaag ctcaggcagc agctcggggc ggtcgccgat cgcgaccagc
     2401 accactcgct ggttcccggt cgaccgcgcc aggcggagca gcatccgctg catcgtcggc
     2461 gtcacacgac aggtgaccac caccatggac gcgccccagg gcaaccgcgg gatctcctcc
     2521 gtcatcatct gctcaatccg ggcaccgaac gccggcgcga tcattgccag tcgctcgagg
     2581 atatgcgcca gctgtcccgg ccgccgaccc ggcgcgatgc gcaccgagcg cgatacttcc
     2641 gggatcggcg cattggcgta catgccgacc tggtagcgtc cctccagcag ctgtgcgctc
     2701 agcgaggccg ccaccaccac ggccagctcg agcaggtcgg actgctgccc ctcccagagg
     2761 tgcgagaacg tgatggtatc gaggaagatc gctgcgatcg gcgtcgccgt cggctccgaa
     2821 acccgcgtct gcagctcgcc gatgcgcgca gtcgcgcgcc agtggatgcg gcgcatgctg
     2881 tcaccggcct cgtactgtcg cacgcccgca atccgcatcg ggtcctccag caatgactgc
     2941 ggcggcttga agtcgccgta cggctggcgc gccggcacga ttacgtgcgt cagcggaacg
     3001 acccgcgggt aggtcaacac tccgtcaacc gcctcgtact gacgctttcg gcgcacaatg
     3061 ccgaagggat cggttgcggt cagggtggcc ggcccgaacc gataccaccc acgccgctgc
     3121 gtgcggaagg tgtagcgata atcgacccgc tcatagaagc cgaggctgaa cagggtgcgg
     3181 aagagccagc ggttgggcac ttccgctcgt tcgagcgccc ggccgggaat ctcaacgttc
     3241 ttcgtgacgt actcggcgac ttcgagccgt gtgaccggca gcggtttgag attgaccgtc
     3301 tcgagtgtca cggtgacctc gtcgccatgg aaggcgcgcc gggtcgagaa cctccgggtg
     3361 taggtggtcg ccacgagggc gaagtcccac cagaggcgca gcgcgagggc gaccagcgcc
     3421 acgccgaccc caacgagcac gagcgccgcc tgcaggaaga cgatgcccaa cagcaccagc
     3481 gccccggcag ctgtcatcca gcgatgaatc tcaatgccga cgttgggcgt gttccaccaa
     3541 gccgtgaagc gatcccacgt gctgcgtgag ttggcgttgt ccatcgcggc catcgggaac
     3601 ggctacctcg cgtcgatcgg ctcggtcggc accgacgtct cgcggacgac ctcctcaacc
     3661 agctgctgct gccccttgcc gagcaggtct gcctgggcgg tcgggatgac gcggtgagcc
     3721 agcacgggca ccgccagttc gcggacatcg tccggcgtca caaacccccg cccctgcacc
     3781 gccgctcgag cctgtgacgc ccgcgccagt gccaggatcg ccctagggct tgccccgacc
     3841 tcgatgtgcg ccgagttgcg cgtgccgcgc acgaggttga gcaggtagtc ttcgacggca
     3901 gggctgacgt ggacgtcctg cacgtggccg acgagctcca tcagggactc aggatcggtg
     3961 accggctgaa ccgactccag gggtccggac gctcgaaacc gtcgcagcat ccggcgctcg
     4021 tcctccgcct ctgggtagcc aatggtgctg cggatcagga agcggtcgag ctgggcctcc
     4081 ggcaacggga aggtcccctc cagttcgacc gggttctgtg tcgcgatcac gaagaacggc
     4141 cggggcagct tcaccagctc ccgttcgact gacacctgcc gctcggacat cgcctccagc
     4201 aacgcactct gcgtgcgcgg ccccgcgcgg ttgatctcgt ctgccagcag cacgttgctg
     4261 atgatcgggc cgggccggta cgtgaactcg gttgtcttcg ggttgaagac gagcgcaccg
     4321 gtaatgtccg acggaagcag gtctggtgtg aactggatgc ggttgaacga cccgccgagg
     4381 gccgccgcca gcgcgcgtgc gagcattgtc tttcccgtac ccggcacatc ctccagaagc
     4441 acgtgaccct cgcacagcac gccgaccagc gtcagctcga tcacctcgcg cttgccgacg
     4501 atcacttgct cgatcgcgct gacgatgcgc gacgtcgttt gctggatctg ctcgatcgcc
     4561 gaggcggcga ggtgctcgct gttatcggtc gccatcaatc gtgaaacccc ttgcaggcgt
     4621 caatccgtgc aggcagtgta cgacaggcca acgcaccatg ggcttgtggc gcccaccaca
     4681 ctgtggaatc agccaacact gaagcccgcc ccgtaggacg agctccagcg cgcaggagga
     4741 tgaaaatgag gtgtgatcac attgtcaggc aggcgcggtg cctggctccg agacggcggc
     4801 agaactcg
//
LOCUS       DASYWL010000116         1841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_5599, whole genome shotgun sequence.
ACCESSION   DASYWL010000116 DASYWL010000000
VERSION     DASYWL010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5599"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="VGR22_04185"
     CDS             complement(<1..668)
                     /locus_tag="VGR22_04185"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEV2127800.1"
                     /translation="MPDITIDYDAIREILLDAGGTAFWVAFWLAAGWWLGRLTRDWVR
                     LALSRRSMGWNGTVLLSRLASVAVRIVAVLLALNSLGVSGTGLLAVVSAFTVAIGLSL
                     QDVLKNFFAGIYLLLERPFRAGDRIVVRDVSGEVQGIDIRTTLIKNRDNELVLVPNAT
                     IFTEILRNDSFYGVRRMELTIKSDSRSVHEIEERMHAALESVEGVKRPLPAPRILAKE
                     DSTLT"
     gene            775..1581
                     /locus_tag="VGR22_04190"
     CDS             775..1581
                     /locus_tag="VGR22_04190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HEV2127801.1"
                     /translation="MFDYHVHSTFSGDCDTPIEATCQAAIATGVTEIAFTDHVDHEPA
                     DPGFGFYDYAGYVEPVAGARDQFGDRLTILAGAEVDFNTRIRDEVERFLDAHDFDFVI
                     GSVHYGDAGEIIFPDYFETRSLDDVFLPYYSELHAAADTGWFDTLGHIDLPKRYRPEL
                     SDAYDPLRYRDALNAIFRTLIARGMSFEINTSGMRQTPATSMPGPQIVELYPRAGGRL
                     VTIGSDSHVAATIGAGFVRTFTMLELTGINEVSRFRKRVREQVPISQLRP"
     gene            1614..>1841
                     /locus_tag="VGR22_04195"
     CDS             1614..>1841
                     /locus_tag="VGR22_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127802.1"
                     /translation="MTPRPPEDPRVSPSPVHRPRRQPRRQRREDRVTEHLPTAATGSA
                     EDRVTRTSIAEGGEVTVRRQQSRRPHPIVLPH"
BASE COUNT          335 a          621 c          573 g          312 t
ORIGIN      
        1 gtgagcgtgc tgtcctcctt ggccaggatt cgcggggcgg gtagtggccg tttgacgccc
       61 tcgaccgatt ccagcgcggc atgcatgcgc tcctcgatct cgtggacgga gcggctgtcc
      121 gacttgattg tcagctccat ccgtcgcacg ccgtagaacg agtcgttccg gaggatctcg
      181 gtgaagatag tcgcgttcgg caccagcacc agctcattgt cgcgattctt gatgagcgtg
      241 gtacggatgt cgatgccttg gacctcgccc gagacgtccc tgacgacgat gcggtccccg
      301 gcacgaaacg gccgctccag cagcaggtaa atgccggcga aaaagttctt cagcacgtcc
      361 tgcagcgaga gaccgatggc cacggtgaag gcgctgacga ccgccagcag gcccgtgccg
      421 gagacgccga gactgttgag cgccagcagg accgccacga tgcggacggc caccgaggcg
      481 agccggctga gcagcaccgt gccgttccag cccatcgacc tgcgggagag ggcgaggcga
      541 acccagtcgc gagtgaggcg tccgagccac caaccagcgg cgagccagaa ggccacccag
      601 aacgccgtgc cgcccgcgtc gagcaggatc tcccgtatcg cgtcatagtc gatggtgatg
      661 tccggcattc gcgctcccgg atttgctgcg ttgctgcact cggagtctta tgcatactcc
      721 tggcaagcga cggcacgtcc gaaatcatcg cacaccaggg agccgtgaga ccccatgttc
      781 gactatcatg tccactcgac gttttccggc gattgcgaca ccccgatcga ggcgacctgc
      841 caagccgcga tcgcgaccgg agttaccgag atcgcgttca cggaccatgt cgaccacgag
      901 ccggccgacc cgggtttcgg cttttacgac tacgctggat acgtcgaacc ggtcgcgggg
      961 gcgagagacc agttcggcga ccgcctgacg atcctcgccg gtgccgaggt cgatttcaac
     1021 acgcggatca gggatgaggt cgaacgcttc ctcgatgcgc atgacttcga tttcgtgatc
     1081 gggtcggtcc actacggcga cgccggggag atcatcttcc cggactattt cgaaacacgg
     1141 tcgctcgacg acgtcttcct gccgtactac agcgagctgc acgcggccgc cgacaccggc
     1201 tggttcgaca cgctcggaca tatcgacctg ccgaagcgat accgacccga gctttccgac
     1261 gcgtacgacc cgcttcgata ccgcgatgcg cttaatgcca tcttcaggac actcatagcg
     1321 cgcggcatgt ctttcgagat caacacctcc ggcatgcggc aaactccggc cacctccatg
     1381 cccggtcccc agatcgtcga gctctacccg cgcgcaggcg ggcggctggt gacgatcggc
     1441 agcgactccc atgtggcagc gacgattggc gccggtttcg tacgtacatt taccatgttg
     1501 gagttgaccg gcatcaacga ggtttcgcgg tttcgcaagc gcgttcgcga gcaggttccg
     1561 atctcgcagc ttcgaccctg atccggctag ggccactgga ggagagtccc cgcatgacgc
     1621 cccgcccacc ggaggatccc cgcgtttcgc cctcgccggt ccatcggccc agacgccagc
     1681 cccgccgcca gcggcgcgag gatcgggtga ccgagcacct gccgacagcg gcaaccggct
     1741 ctgccgagga ccgggtcacg cgcaccagca tcgcggaggg aggcgaggta accgttcgac
     1801 gacagcagag ccggcgtccg catccgatcg tcctgccgca c
//
LOCUS       DASYWL010000117        18453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_587423, whole genome shotgun sequence.
ACCESSION   DASYWL010000117 DASYWL010000000
VERSION     DASYWL010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 18453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18453
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587423"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..1021
                     /locus_tag="VGR22_04200"
     CDS             56..1021
                     /locus_tag="VGR22_04200"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEV2127803.1"
                     /translation="MRESALPPAKEIASFREALLGWWEGNARDLPWRQTRDPYRILVS
                     EVMLQQTQVDRVIPYYHQWLRAFPDVYALAAAPTAEAIRLWKGLCYNRRAVNLQRAAV
                     AVVERGGQFPETVEELLALPGIGPYTAGAIACFAFERDAPFIDTNIRRVLHRVFVGSD
                     VPERRARDREILAIAQASLPHGDAWRWNQALIEFGALQCTARTPACVICPAQAVCMAR
                     PTIHSDLQAASAERKSRNKAPVPFERTNRYFRGRIIDALREHNGAAMPIEALGRMIKP
                     DYSVEDQAWLQKLVVDLERDGLATIAEDEPEYDAGDKQPLRVRLP"
     gene            complement(1317..1997)
                     /locus_tag="VGR22_04205"
     CDS             complement(1317..1997)
                     /locus_tag="VGR22_04205"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEV2127804.1"
                     /translation="MQVNRDKLLFFGVLALIVVLVAGGFVVRETWDRAPGEDSAEAGF
                     LRDMQVHHTQAVEMAMIIRDRTEDEQIEAMATDIAFSQTSQIGQMQGYLLIWNINPTG
                     DEPAMAWMGHEVEGRMPGMASEEQINALQELPVDRAETLFLQLMNRHHLAGVDMAEAV
                     IERSDNDEIEELAASMVRVQAAEIEVMNQFLAERGYEPVTLENMDEIDLVTGDVPDDA
                     KAEEHGGH"
     gene            complement(2199..3032)
                     /locus_tag="VGR22_04210"
     CDS             complement(2199..3032)
                     /locus_tag="VGR22_04210"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HEV2127805.1"
                     /translation="MGNEHGSRAGDQPSNVHRKRAERRPEIVKRRREQRRREFEKQQR
                     NWLILRMVAGVVLVAIVVAIAWWGYQQWEEYQVSQDVEVFFGIDDYAADHVEGEVLYE
                     QVPPVGGPHNNIWQNCGFYDEPIYNWHGVHSLEHGAVWITYDPDLPEEDIEQLRDLGN
                     QTYVLVSPYPGLEHPVVASVWGKQIALEGVGDERLRPFIREYRTNPDNTPEPGALCSL
                     GVDYTIDDGFSVQSVPAEVSGVAETDAEADEPEAISDQPEVDESTRDRVVASPEAAAE
                     E"
     gene            complement(3101..4519)
                     /locus_tag="VGR22_04215"
     CDS             complement(3101..4519)
                     /locus_tag="VGR22_04215"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEV2127806.1"
                     /translation="MTQDVKTRVATMDKVVALAKRRGFVYPGSDIYGGLANTWDMGPL
                     GVELKNNIKQLWWWTFVQRRRDMVGLDASILMHPKVWEASQHVASFSDPLVDCKTCKA
                     RFRADHLLEEKLGRMDAEALTPDELTAVLREANLPCPNCGNRTLTDARQFNMMFRTTI
                     GPVSETGTEVYLRPETAQGIFVQYKNIMQTSRVKLPFGVGQIGKSFRNEITPGNFIFR
                     VLEFEQMEIEYFIREAEWEQHFDSWVAAMSDWIDAIGLDREHVRLRSHSEQELSHYSR
                     RTVDYEYDFPEPMGWKELYGLAYRTDFDLRQHQETSGEDLTYFDQESRQRFLPHVIEP
                     TFGVERTFLTVLLDAYDEEMTTDVNGKQDTRTVLHLDPKLAPMKAAILPLAKKPELTS
                     VAWELFERVQDGLGLMVDYDETANIGKRYRRQDEIGTPFCVTVDFDTLEDQAVTIRDR
                     DSMEQVRVGLSEVASWLRERIT"
     gene            complement(4526..5476)
                     /locus_tag="VGR22_04220"
     CDS             complement(4526..5476)
                     /locus_tag="VGR22_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV2127807.1"
                     /translation="MKSKIDAFLEYLERERAFSENTISAYRNDLNQLASFLADPPAES
                     GLGPVTDIDDLRDGHLYAFADHLSKRDYASSTVARKMAALKSFANYLHRSETTNELLG
                     RELVVPRVKKARPRAISPDEVERLLDAPLAQQPARPEQIRDLAMLELLYASGLRVSEL
                     VSLNVDDVDFDNLTLRLRGKRGKDRQVPLPKRSVKALQTWLGDPRNAIANRGETSLFV
                     NHRGNRLTRQGFWLILKSYANQVGIEEITPHTLRHSFAAHAVDRGMDLKELQRRLGHV
                     SSVTTQVYQQMRKEQANGSGNVEGREVDYDDLTRVPAGVE"
     gene            complement(5610..6737)
                     /gene="ald"
                     /locus_tag="VGR22_04225"
     CDS             complement(5610..6737)
                     /gene="ald"
                     /locus_tag="VGR22_04225"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEV2127808.1"
                     /translation="MIVGIPKEVKDRENRVSATPAAVHEYIANGHEVIVETSAGMGSG
                     FPNDEYIAEGAQIVGSHEEVFAVADMIVKVKEPVVSEYELLREGQILYTYLHLAADEP
                     LTRALVDRKVQAIAYETVQLPNRHLPLLAPMSEVAGRMSVQVGAHFLESPHGGLGMLL
                     GGVPGVQPAEVVVIGGGAVGTNAAKMALGLGANVTIIELSLDRLRYLDDVLHGRINLL
                     ASNRKTIADAVVKADLVVGSVLIPGARAPKLVTADMVSTMKAGSVMVDVAIDQGGCFE
                     TSKPTTHSDPTYVVDGVIHYCVTNMPGAVARTSTLALSTVTVPYGIKLANQGFVEAVT
                     SDEALAKGVNVANGKVTHEGVAEAFGMPHIPLDEALSTHAA"
     gene            6946..8637
                     /locus_tag="VGR22_04230"
     CDS             6946..8637
                     /locus_tag="VGR22_04230"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEV2127809.1"
                     /translation="MAATSGAPVTLHYTPHPLPYGALSLEIPPLVPEISDGLLAGLLI
                     VPMLAFIILAHEFGHFFAARSVGIKVEEFGLGIPPRAKGWTWRGVLWSLNWIPLGGFV
                     RVKGEDGRDMEPGSMNTKGPASRAFFLLAGSGANFVAAALLSIMLVAAQGVPNETSQI
                     YIQQVEPNSPAAEAGWQPADAIVAIAGEPIDGLAEFSAALDRYAGQEMPVVIERGDQR
                     IATTVTPREDPPAGQGATGIRIGEAYLSSIQIGEVSPGSPAAEAGWQEGDEIVAIDGV
                     EIESVAQVNALLSNAAGGVTEVTMLRGGELVETMISLEQPAIEITAVGPDSPASEALL
                     YPGDRLVSIGGMETADPMSLFLALEDAAGGEVPVVVEREGREVELTLAVPEYDEGENP
                     LAVIGANAMTPTWQSQAGVSTQSSRDFVAVDASETIPRGLDQFWFIVTGTLEGIGQLF
                     TAPDTGNLAGPVGMGQLTSEFIAESSMPVWFIVGNLVMVISVGLGLLNLMPLPALDGG
                     RLAFVVLEVLRGGKRLPPEKEGLVHLAGLALLLMLMFFVAFNDVTRIIDGEQILP"
     gene            8888..10066
                     /gene="ispG"
                     /locus_tag="VGR22_04235"
                     /gene_synonym="gcpE"
     CDS             8888..10066
                     /gene="ispG"
                     /locus_tag="VGR22_04235"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEV2127810.1"
                     /translation="MSLIAPRRKTRVLNVGGVEIGGQNPIRVQSMATADTRDPQATIS
                     QIQDLADVGCEIVRIAVPDRKAAAALPEIVPYAAVPLIADIHFEHTLALKALDAGIHG
                     LRLNPGNIRKPEDVREVVTRAKERGAPIRIGVNFGSVAPMTEEFVDEMAQQNATQVEL
                     RAEWLVRSALGHIRILEDLDFYDTKVSLKAFEVPVLVEAYRRFARLDNDYPLHLGVTE
                     AGTPRAGSVRSAVGIGTLLAMGIGDTVRVSLTTDPKEEVFVAYEILKSLELRQKGATM
                     IACPTCGRVEVDLFKLANQVDKVLEGIQEPIRVAVMGCVVNGPGEARDADVGIASGNG
                     KGVIFRKGKIVKTVLEADMLDELTNEINAVVEDRKNGIFDEPEDGAGAYRPTIPLTVV
                     "
     gene            10170..11621
                     /locus_tag="VGR22_04240"
     CDS             10170..11621
                     /locus_tag="VGR22_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEV2127811.1"
                     /translation="MTASTEDDRSTKAISALRRASVLAERWLADLPDRPIDAGATPEQ
                     MAKRLDGPLPETGLAPDDAVAEWLDHAEPGIVPINGPRYFGFVMGGTLPAALGGDILT
                     STIDQNSGMWSMSPASAQTELTVIRWLKELFGLPPDWMGVMTSGATMSNLVGLAAARQ
                     WAGEKLGFNAAEDGLGGQPAITIVSSDAIHVSARKALGNIGLGRRSVRTVPAPEGHAD
                     IDALADLLHTVDGPVIVVGNAGEVNTGQFDDLQAFAELCRNHPGGAWLHVDAAFGLFA
                     AASSDHRHLLAGIEHADSVGADAHKWLNVPYGSGFAFVRDEEVLRAAFATTGAAYLAA
                     SGGWGAESYSPEMSRRARAIPAWCALRSLGREGYRELVDRCIGNARKLAEWVDAESGL
                     ELMNADRMRETPFMIVCVRFTNPSWDDEEHDRRNRERLASLQADGRVYASTTVWDGKA
                     AMRFAFDNWMTTEADVETICAVLRELRDQQTGA"
     gene            11809..12942
                     /locus_tag="VGR22_04245"
     CDS             11809..12942
                     /locus_tag="VGR22_04245"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2127812.1"
                     /translation="MLDFMRTGSLRASVVLLAALIMAMMPSAGFSPAAAQDALACQPI
                     PKATPTGTLTRDVETPEFEVPEDAVPVTVGYTPISIFAPMFVAYEKGYFAEQGLDVTL
                     EALPGGTDMVVHVATGNFDAGIGGIGPAYWNAEAQGLPVTVIAPGHMEEAPVASPLMI
                     STEACESGEIMQVSDLEGKKVAVNAPGATEIWLDAALRTGGLTIEDIDLQYMPFPDAV
                     AALDSGAIAASIVGEPLATQAENEGIAVRLASDFDVADYQVTAIFANDEFLAEHPEAA
                     TGLVTAYLKASRDLTEDFSDLLHLAIIEEYTGVPASLVAESVKPVYELDGEIDLESLA
                     DVQEFFRNRGLLEYEESIDPASMVDQTFVDRAIENIGENQADS"
     gene            12939..13793
                     /locus_tag="VGR22_04250"
     CDS             12939..13793
                     /locus_tag="VGR22_04250"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2127813.1"
                     /translation="MTEIVLPTVSNRRLPTRANEAIVSTTRVGKTFETPRGPVRAVED
                     VTLAFAPGSFNVIVGPSGCGKSTLLRMIAGLESPTSGDLSFTRAQENASAPTNALVFQ
                     GRSLFPWLNLRQNVAYGLRLQGISRAERSAKADELLHLVGLRKFSRSYPHQVSEGMRQ
                     RVAIARALAVDPDILLMDEPFGALDEQTRLLLQEELLRIWEATGKTVIFVTHSLDEAL
                     LLADTIVVMSAQPGTVRTVFRVPFPRPRTLTEVRKDPKFAELFSRTWEILRTEVNAAR
                     ARQEQEIA"
     gene            13793..14566
                     /locus_tag="VGR22_04255"
     CDS             13793..14566
                     /locus_tag="VGR22_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2127814.1"
                     /translation="MSLRQRRASELALTLLAPLLLLSIWELLSRSELINPLYWPAPTS
                     LWSTFVELLTEGDLLTDIWLSTVRILGGFLLGSIPGIVLGLAMGLFWPVRVFLMPLAA
                     ALYAIPKIAILPLVMIIFGIGEASKFMIVALSIFFLVVLNTMSGVLEIDRTFRDVARN
                     LGASRLELFTTVALPGALPSIFTGLRLAMGFALIVIVGTEFLTVSQGGIGAMIWQSWT
                     ILSVKKMMVGLIITGIMGWLLSLLVSSAERLVMPWRVAD"
     gene            14566..15513
                     /gene="menA"
                     /locus_tag="VGR22_04260"
     CDS             14566..15513
                     /gene="menA"
                     /locus_tag="VGR22_04260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HEV2127815.1"
                     /translation="MSTTTPTPAATDCTTRPPLWKVWLVAVRPFSFTTSTVPVIAASM
                     LALYEGAVNVWALILMLIASISTHAGCNLANDYYDDETGVDKKQVIGQAGVLQANWLT
                     RREIGFGIIVAFAVALVAALPIMIDIGRPIVILALLSAAAAYFYTGGPFPLAYWALGE
                     VTVFLAMGVGMVVGTYYVHTGAVSTIAVLLSVAIGCVVTAILHANNIRDREVDRQHRK
                     RTIANLLPRGFANAEFQVLVLVPFALIAAMIVLDPGSWPIAVSALALPRALFLARTIA
                     DADTPRELNPYVRQSAGLHMQFGLLITAGYIAARLIEML"
     gene            15510..16412
                     /locus_tag="VGR22_04265"
     CDS             15510..16412
                     /locus_tag="VGR22_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV2127816.1"
                     /translation="MTFSFKWAAAIALGGLASVGAAVGYWSRFVHPYRTQLTHEIMAV
                     PRKHARLNGLTIAFVSDTHIGPHFRTDDLRPAIEMLRRAQPDILLLGGDYVSESPRFI
                     DPSVDALLDMAATARFGTWAILGNHDLANTPERVVEALERAGIPTLINDAAHAETDRG
                     DLWIVGIDDALLGEPDLKAAFSGIPADAPVVAMWHEPDVAEDMLPFDPLFMLSGHTHG
                     GQIRIPGLVDAAVPRLGKRYPLGRYEVQGMPLYVSSGVGMYRPPVRFNCPPEVVIITL
                     LGEQDGADMLSSQLPARGTLRAEM"
     gene            16484..17599
                     /locus_tag="VGR22_04270"
     CDS             16484..17599
                     /locus_tag="VGR22_04270"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2127817.1"
                     /translation="MSNEQTVRVGVIGCGAGIFHLNGYSKDPRVKIVALAGLDTDRCE
                     RLAKDFEIPRVYTHYQDLLGDSDIDAVSVVVPNHLHLPVSKAAFEAGKHVLVEKPLAG
                     TVADGEQMVALAKEHGKLLGIAFQRRWRHDVQIVRELIENGEMGDVYHAKAFWMRRSG
                     IPGWGSWFTSKEAAGGGPLIDLGVHVLDMALYMLGNPKIVSVSAATYDKIGSQGKGNW
                     PGTPGARTGGSNAYEVEDFSTALLRFKDGSTLALDTAWAAYTEMTDEFGVQVFGSNGG
                     AKIHSKEYADVGTLQLFSDVAGTATDAKPRLQSRNGHEYICRHFVDAVLNGTPVSPSG
                     EEGLDRVRIIEAIYRSAALGREVLIDEVIEPVQDAAD"
     gene            17672..18439
                     /locus_tag="VGR22_04275"
     CDS             17672..18439
                     /locus_tag="VGR22_04275"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEV2127818.1"
                     /translation="MPSEIRVTVWNEFRHEKKDPEVGEVYPDGIHGAIAEGLNEAQGI
                     TARTATLDEPEHGLPQDVLDNTDVLTWWGHMAHREVDDAVVERVHERVMAGMGLIALH
                     SAHFSKIFKKLMGTTCDLKWREADEKERLWVVRPGHPIVEGIGEYIELDKEEMYGEHF
                     DIPQPDELIFVSWFQGGEVFRSGATYNRGAGKVFYFRPGHETYRTFYNPEIRRVIANA
                     VRWAAPVERPARTFGNSDPIEDLPWYDKQHAVTKGYR"
BASE COUNT         3174 a         6223 c         5777 g         3279 t
ORIGIN      
        1 gccttccaca tcggttcgag ccgtgaacca tgcctttccc aagtgacatt gcccaatgag
       61 ggaatccgcg ctgccacctg ccaaagagat tgcgtcgttc cgcgaggccc tgctcggctg
      121 gtgggagggc aatgcacgcg acctcccgtg gcgacagacc cgcgacccct accgcatcct
      181 cgtctcggag gttatgctcc agcagacgca ggtcgatcgc gttatcccct actatcacca
      241 gtggctgcgg gcgtttcctg atgtctacgc gcttgccgcc gccccgacag ctgaggcaat
      301 ccgcctctgg aagggactct gctacaaccg gcgggcggtc aacctgcagc gggccgccgt
      361 ggcggtcgtc gagcgtggtg gtcagttccc ggaaaccgtg gaggaactgc tggcgctgcc
      421 tggcatcggt ccgtacaccg ccggggcgat tgcctgcttc gccttcgagc gtgacgcgcc
      481 ctttatcgac accaacatcc ggagagtcct gcaccgggtc ttcgtcggca gcgacgttcc
      541 cgagcgccga gcccgtgacc gggagatcct cgcgatcgcc caggcgtcgc tgccgcatgg
      601 agacgcctgg cgatggaacc aggcgctcat cgagttcggc gcgctgcagt gtaccgcccg
      661 gacaccggcc tgtgtcatct gcccggcgca ggcggtctgc atggcacgtc cgacaatcca
      721 cagcgacctc caggcggcca gtgccgagcg gaagagccgc aacaaggccc ctgtcccgtt
      781 cgagcggacg aatcgctact tccggggacg aatcattgat gccctccgag agcacaacgg
      841 tgcagcgatg ccgatcgagg cgctgggacg catgatcaag ccagactact cggtcgaaga
      901 ccaggcgtgg ctgcagaagc tggtcgtcga cctggagcgc gacggcctcg ccaccatcgc
      961 cgaggacgag ccggagtacg acgctgggga caagcagccg cttcgggtca ggctgccgta
     1021 gggtcggtcc cgttgggctt gacatgaaga ctcgcggcta gaacgatccg gtttaatccg
     1081 tttccgaggt cgtgggttga tcggtgagaa tctatccgcg catctgagca ccggtctcca
     1141 cggccggcca caaggacggc ggccgggccg acgcctgctg cacgggtggc cccagcatgg
     1201 cctactgcgg tcttttccag gatgttgctg agtctcgcta tcggtatcgg tcccgcccat
     1261 tgtcagggag gggcggcatg tctccacgcg gttggtcgct cgttccttgc gttggatcag
     1321 tgccccccgt gctcttcagc cttcgcgtcg tcgggcacgt caccggtcac caggtcgatc
     1381 tcgtccatat tctctagcgt caccggctcg tagccgcgct cggccagaaa ctggttcatc
     1441 acctcgatct cggcggcctg aacgcggacc atcgacgccg ccagctcctc gatttcgtcg
     1501 ttgtcgctgc gctcgatcac cgcctcggcc atgtccaccc cggcgaggtg gtgccggttc
     1561 atcaactgca gaaagagcgt ctcggcccga tcgaccggaa gctcctgcag ggcgttgatc
     1621 tgctcctcgg aggccatgcc cggcatgcga ccctcgacct cgtgccccat ccacgccatc
     1681 gccggttcgt cccccgtcgg gttgatgttc cagatcagca ggtagccctg catctggccg
     1741 atctgcgacg tctggctgaa ggcgatatct gttgccatgg cctcgatctg ctcgtcctcg
     1801 gtgcggtcgc ggatgatcat ggccatctcc accgcctgcg tgtgatgcac ctgcatgtcg
     1861 cgcaggaatc ccgcctcggc ggagtcctcg cctggcgcac ggtcccacgt ctcccggacg
     1921 acgaagccac cggcaaccaa cacgacgatg agcgccagca cgccgaagaa gagcaacttg
     1981 tcccgattca cttgcatgat ggacgtcccg attgtttctg caccacgatc tgctccactg
     2041 cctcctcgac tctaccaccg gtttcgcctc ggtcgtgcgg cgaaaccggt gcggatgcca
     2101 aatatgcgaa ggcgttacgg tatccacaga acaccggcgg gccagttggg taaccggccc
     2161 gccggtgttc tcctgatcaa ggtcaacctg gtccgagatt actcctccgc ggcagcctcg
     2221 ggcgaggcca cgacccgatc cctcgtagac tcgtccacct ctggctggtc ggagatcgct
     2281 tcaggctcat ccgcttcggc atccgtttca gccacaccgg agacctccgc gggcaccgac
     2341 tgcacactaa agccatcgtc gatcgtgtag tccacaccga ggctgcagag cgcgccaggt
     2401 tcgggcgtat tgtcgggatt ggtccggtac tcgcggatga acgggcgcag gcgttcgtca
     2461 ccgacccctt cgagggcgat ctgcttgccc cagaccgatg cgaccaccgg gtgttcgagg
     2521 cccgggtacg ggctcaccag cacgtaggtc tggttcccca ggtcccgcaa ctgctcgatg
     2581 tcttcctcgg gcaaatccgg gtcgtaggtg atccagaccg cgccgtgctc gagcgagtgg
     2641 acgccgtgcc agttgtagat cggctcatca tagaacccgc agttctgcca gatattgttg
     2701 tgcggcccac caaccggggg aacctgctcg tagagcacct cgccctcaac gtggtcggcg
     2761 gcatagtcgt cgatgccgaa gaagacctcg acatcctgcg acacctggta ttcctcccat
     2821 tgctggtagc cccaccaggc aatcgccaca acgatcgcca ccagcaccac gccggcgacc
     2881 atgcgcagga ttagccagtt tcgctgctgc ttctcgaact cacgccgtcg ctgctcgcga
     2941 cggcgcttca cgatttccgg acgacgctcg gcacgcttcc ggtggacatt gctcggctgg
     3001 tcccctgcgc gggacccgtg ttcgttcccc atcgttctcc agttctctca gatggtaatc
     3061 acccatcagt ccgctcgtcg cgacaggtga ggtcagccgc ctaggtgata cgctcccgga
     3121 gccagctcgc tacctcggac aggccgacgc gcacctgctc catggagtcg cggtctcgaa
     3181 tggtaacagc ctgatcctcc agcgtgtcga agtccaccgt tacacagaac ggcgtgccaa
     3241 tctcgtcctg ccgccggtag cgcttgccga tgttggcggt ctcatcatag tcgaccatca
     3301 ggccaaggcc gtcctgcacg cgctcgaaaa gctcccatgc gactgaggtg agctcgggct
     3361 tcttcgcgag tggcaggatg gcggccttca tcggtgccag tttcggatcg agatgaagca
     3421 cggtccgggt gtcctgcttg ccgttgacgt cggtggtcat ttcctcgtcg tacgcgtcga
     3481 gcagtacagt gaggaacgtc cgctccacgc cgaaggtcgg ctcgatcacg tggggcagga
     3541 agcgctggcg agactcctga tcgaagtacg tcaaatcctc gccgctggtt tcctgatgct
     3601 gccggaggtc gaaatcggtc cggtaggcaa gcccgtagag ctccttccag cccatgggct
     3661 cggggaagtc gtactcatag tcgactgtgc gccttgagta gtgtgagagt tcctgctcgc
     3721 tgtgcgagcg cagtcggaca tgctcgcggt cgagaccgat agcatcgatc cagtcgctca
     3781 tggccgccac ccagctgtcg aagtgctgct cccactcggc ctcccggatg aagtactcga
     3841 tctccatctg ctcgaactcg agcacgcgga agatgaagtt tccgggcgtg atctcattgc
     3901 ggaaggactt gccgatctgg ccgacgccga agggtagttt cacgcgagag gtctgcatga
     3961 tgttcttgta ctgcacgaat atgccctggg ccgtttccgg ccgcaggtag acctccgtgc
     4021 cggtctcaga gaccgggccg atcgtcgtcc tgaacatcat gttgaattgt cgggcgtcgg
     4081 tcagcgtgcg gttgccgcag ttcgggcacg gcaggttcgc ctcccgaagc accgccgtca
     4141 gctcgtccgg cgtgagcgcc tcggcgtcca tccgcccaag cttctcctcc agcaggtgat
     4201 cggcacggaa gcgcgccttg cacgttttgc agtcgaccag cggatcgctg aagctcgcca
     4261 cgtgctgcga ggcctcccac accttcgggt gcatgaggat cgaggcgtcc aggccgacca
     4321 tgtcccgacg ccgctgcacg aacgtccacc accagagctg cttgatgttg ttcttcagct
     4381 caacgccgag cggacccatg tcccatgtgt tggcgaggcc gccgtagata tcggaccccg
     4441 gatagacgaa cccacgacgc ttcgccagcg cgacgacctt gtccatcgtc gcgacccgcg
     4501 tcttgacgtc ctgtgtcatg cggtgctact ccaccccggc cgggacgcgg gtgagatcgt
     4561 cgtagtcaac ctcgcgacct tcgacgttcc cagacccgtt cgcctgctcc ttgcgcatct
     4621 gctggtacac ctgcgtggtg acgctgctga catgcccgag gcgccgctgc agctccttga
     4681 gatccatgcc gcggtcgaca gcatgcgccg caaaggagtg ccgcagggtg tgtggggtga
     4741 tctcctcaat cccaacctga ttggcgtagg acttgaggat caaccagaag ccctgtcgcg
     4801 tcagtcggtt gccccgatgg ttgacgaaca gcgacgtctc gccacggttg gcaatcgcgt
     4861 tgcgcggatc gcccaaccaa gtctgaagcg ccttcacgct gcgcttcggc agcggcacct
     4921 ggcggtcctt gccgcgcttg ccgcgcagcc gcagcgtgag gttgtcgaaa tcgacgtcgt
     4981 caacgttgag cgagaccagc tcgctgacgc gcagaccgga cgcgtagaga agctccagca
     5041 tcgcaaggtc acgaatctgc tctggacgcg ccggctgctg cgcgagcggc gcgtccagca
     5101 gccgctcgac ctcgtccgga gagatagcgc gcggccgcgc cttcttgacc cggggcacca
     5161 ccagctcacg accgagcaac tcgttggtcg tctcggaacg gtgcaggtaa ttcgcgaagg
     5221 acttcaacgc ggccatcttg cgagcgaccg tcgaagatgc gtaatcccgc ttgctcaggt
     5281 gatccgcgaa ggcatagaga tgaccgtccc gcaggtcgtc gatatcggtc accggcccca
     5341 acccggattc cgccggtggg tccgccaaga acgacgcgag ctgattgagg tcgttccgat
     5401 aggccgaaat cgtgttttca gaaaatgctc gttcgcgctc cagatactcc agaaacgcat
     5461 cgatcttgga cttcacgtaa tgcccccttg gacgatatgt agtgcctgtt cagctattgt
     5521 cagccagacc tcgcttgcgt ggcgaaaccg accgtggtcc tcccccgcag tgctcgcgac
     5581 cggttcagac ataaccatac tacgcgttgt cacgcggcgt gtgtcgaaag cgcctcatcg
     5641 agcggaatgt gcggcatacc gaacgcttct gcgacgcctt cgtgggtgac cttgccgttc
     5701 gccacgttga cgcccttggc gagcgcctcg tcactcgtga cagcttcgac gaagccctga
     5761 ttcgccagtt tgatcccgta tgggacggtc acggtggaaa gcgccagtgt gcttgtgcgg
     5821 gcgacggcac ccggcatgtt ggtcacgcag taatgaatca cgccgtcgac gacgtaggtt
     5881 ggatcactat gcgtcgtggg cttcgacgtt tcgaagcagc ccccctgatc gatggcgaca
     5941 tcgaccatca ccgatccggc cttcatcgtc gacaccatgt cggcggtgac gagcttcggc
     6001 gcccgtgccc ccggaatcag caccgagccg acaaccagat cggccttcac caccgcatcg
     6061 gcgatcgtct tgcggttcga cgccagcagg ttgatgcgcc cgtggagcac atcatccagg
     6121 taccgaagcc ggtcgaggct cagctcgatg atcgtgacgt tggcacccag gccgagcgcc
     6181 atcttggcgg cgttggtgcc gactgctccc ccaccgatca ccacgacctc cgcgggttgc
     6241 acgccgggca cgccgcccag cagcatgccg agccccccgt ggggactctc gagaaaatgg
     6301 gcgcccacct gtaccgacat acggccagcg acctcgctca ttggcgccag caacggaagg
     6361 tgacggtttg gcagctggac cgtctcgtag gcgatcgcct gcacctttcg atcaaccagg
     6421 gcacgcgtga gcggctcgtc ggcggcgaga tgcaggtagg tgtagagaat ttgcccctcg
     6481 cgcaacagct cgtactcgct gacgaccggc tccttcacct tgacgatcat gtcggccacg
     6541 gcgaacacct cttcgtgcga gcccacgatc tgcgcacctt cggcgatgta ctcgtcattc
     6601 ggaaaaccgc tgcccatccc tgccgatgtc tcgacaatga cttcgtgtcc attggcgatg
     6661 tactcgtgca cggcggccgg cgtggccgaa acccggttct cgcggtcctt cacttccttg
     6721 ggaatcccga cgatcatgca ggcctctcct gtacagcccc tcacggtcca tcacacgcgc
     6781 aacggtccga tcctgaccaa ccagctatga cagcgctcac gccggagcga gcgcactgac
     6841 cccataacgg ccgtcgatcg tcccgcacgg ttgcacagtc gccgcattct agcacggcta
     6901 ccgccgtgtt ccggagcaga ttcgccgaca tagtaagatt gcgccgtggc tgccacatcc
     6961 ggcgcaccgg tcaccctcca ctacacgcca cacccattgc cctacggtgc gctctcactg
     7021 gagatccccc cactcgtgcc cgagatttcc gacggcctgc tggccggctt gcttatcgtc
     7081 ccgatgctcg cgttcatcat cctcgcccat gagttcgggc acttcttcgc cgcccgctcg
     7141 gttggaatca aggtcgagga gttcggcctc ggcatcccgc ctcgcgccaa gggctggaca
     7201 tggcggggtg tgctatggtc gctgaactgg atcccgctcg ggggcttcgt ccgcgtcaag
     7261 ggcgaagacg gccgagacat ggagcccggc tcgatgaaca ccaagggtcc tgcctcacgc
     7321 gcattcttcc tgcttgccgg ttctggcgcg aacttcgtcg cggcggcgct gctctcgatc
     7381 atgctcgtcg ccgcccaagg cgtaccgaac gagacgagcc agatctacat ccagcaggtc
     7441 gagccaaact caccggcggc cgaggcaggc tggcaacccg ccgacgccat cgtcgcgatc
     7501 gccggggagc cgatcgatgg cctggcggag ttctccgccg cgcttgaccg ctacgccggg
     7561 caggaaatgc ccgtcgtgat cgagcgcggc gaccagcgca tcgcgacgac cgtcacgcca
     7621 cgcgaagacc cgcccgcggg ccagggagcg accggcatcc gcatcggcga ggcatacctc
     7681 tcatccatcc agatcggtga ggtctccccc ggctcgcccg ccgccgaggc cggttggcag
     7741 gagggcgacg agatcgtcgc gatcgacggt gtcgagatcg aatcggtcgc gcaggtaaac
     7801 gccctgctca gcaatgccgc aggcggcgtg accgaggtca ccatgctgcg cggtggcgag
     7861 ttggtggaga cgatgatctc gctggaacaa ccggcgattg agatcacggc ggtcggcccg
     7921 gactcgcctg cgtcggaagc gctcctttac cccggtgacc gtctcgtctc tatcgggggc
     7981 atggaaaccg ccgatccaat gtcgctcttc ctcgcgctcg aggacgcggc ggggggcgag
     8041 gtgccggtgg tggtcgagcg tgagggccgc gaagttgagc tgacgctggc cgtccccgag
     8101 tacgacgagg gcgagaaccc gctcgccgtg atcggcgcga acgcgatgac gcctacctgg
     8161 cagtcacagg ctggcgtttc gacgcagtcg agtcgtgact tcgtcgcggt ggacgcgagc
     8221 gagaccatcc ctcgggggct ggaccagttc tggttcatcg tcaccggcac cctcgagggc
     8281 atcgggcagc tcttcaccgc gccggacacc ggcaacctcg ccggcccggt cggcatggga
     8341 cagctcacct cggagttcat cgccgagtcg tcgatgccag tctggttcat cgtcggcaac
     8401 ctggtgatgg tgatctcggt cggactcggt ctgctcaacc tgatgccgct gcctgcgctc
     8461 gacggcggcc gtctcgcgtt cgtggtgctt gaggtgctgc gcggtggcaa gcggctgcca
     8521 ccggagaagg aaggactcgt ccatctcgcc gggctggccc tgctcctgat gctgatgttc
     8581 ttcgtcgcct tcaacgacgt cacccgcatc atcgacggcg agcagatcct gccgtgaacg
     8641 ggcatccgta ggggaggaca ttgaccgccc ttggaaccgt atcaccgcga cgagggactg
     8701 cgcggagcat tggcgtccat cgacctttga gcgcagccga gtcgtcccct acggatcgac
     8761 ggatcggtgc catccacacc ttgctcacac ccccgggcaa ccgaacgtcg taccatagac
     8821 gaagcagata gcgacaagcg acggttcctc gaccgccgct cgcgcatgac ggaggaacca
     8881 cgtactcatg tccctgattg ccccacgccg caagacgcgc gtgctcaatg ttggcggcgt
     8941 tgagatcggc ggccagaacc cgatccgcgt gcagtcaatg gcgacggccg acacccgcga
     9001 cccgcaagcc acgatctccc agatccagga cctcgccgac gtcggctgcg agatcgtccg
     9061 catagccgtg ccggaccgca aggcggccgc cgcgctgccg gagatcgtgc cctatgcggc
     9121 cgtcccgctg atcgccgaca tccatttcga gcacactctc gcgctcaagg cgctcgatgc
     9181 tggtattcac gggctgcggc tcaacccggg caacatccgc aagcccgagg atgtccgcga
     9241 ggtcgtcacc agggcgaagg agcgaggcgc ccccatccgc attggcgtca acttcggctc
     9301 ggtcgcaccc atgaccgaag agttcgttga cgagatggcg cagcagaacg cgacgcaggt
     9361 cgagttgcgt gcggagtggc tggtgcgatc ggcgctcggc cacatcagga tcctcgaaga
     9421 cctcgacttc tacgacacca aggtcagcct caaggcgttc gaggttccgg tgctggtcga
     9481 ggcgtaccga cgcttcgccc gcctggacaa cgactacccg cttcacctcg gggtcaccga
     9541 ggccggcacg ccacgggcgg gcagcgtccg cagcgcggtg ggcatcggca ccctgctggc
     9601 gatgggcatc ggcgacacgg tccgcgtctc gctgaccacc gacccaaagg aagaggtctt
     9661 cgttgcctac gaaatcctca agagcctcga gctccgacag aagggcgcga cgatgatcgc
     9721 ctgcccgacc tgcgggcggg tcgaggtgga cctcttcaag ctcgccaacc aggtcgacaa
     9781 ggtcctagag ggtattcagg agccgattcg cgtcgccgtg atgggctgcg tggtgaacgg
     9841 cccgggcgag gcgcgcgacg ccgacgtcgg catcgcctcc ggcaacggca agggcgtcat
     9901 cttccgcaaa ggcaagatcg tcaagacggt gctcgaggcg gacatgctcg atgagttgac
     9961 gaatgagatc aacgctgtcg tcgaggaccg caagaacggc atcttcgacg agccggagga
    10021 cggcgctggc gcctaccgac cgacgatccc gctgacggtg gtgtagccca aacctagccg
    10081 cgctcgcatg agccaagcgc aaccgaacag tcgaatgggg cacccggact gcgccacgca
    10141 cccgggtgcc attgcatccg gaggaaccga tgaccgcttc cacagaggat gatcgcagca
    10201 ccaaggccat atccgccctt cggcgcgcca gcgtgctggc cgagcgctgg ctcgcggacc
    10261 tgcccgaccg accgatcgac gccggggcaa cccccgaaca gatggccaag cgcctggacg
    10321 gcccgctccc ggaaaccggc ctcgcgccgg atgacgccgt cgccgagtgg ctggaccacg
    10381 ccgagcccgg cattgtccct atcaacggtc ctcgctactt cggcttcgta atgggcggca
    10441 ccctccctgc cgcactcggc ggcgatatcc tcaccagcac cattgaccaa aacagcggta
    10501 tgtggagcat gagcccggcg tccgcgcaga ctgagttgac cgtgatccgc tggctcaagg
    10561 agcttttcgg cctcccaccg gactggatgg gagtgatgac gagcggcgcc acgatgagca
    10621 acctcgtcgg actcgccgcc gcgcggcagt gggctggcga aaagctggga ttcaacgcgg
    10681 cagaggacgg tctcggcggt caacccgcca tcacgatcgt ctccagtgac gcgatccacg
    10741 tcagcgctcg caaggcgctc ggcaacatcg gtctcggccg ccggtcggta cggacggtgc
    10801 ccgcacccga gggccacgcc gacatcgacg ccctcgccga tctgctccac accgtggacg
    10861 ggccggtgat cgtggtcggc aacgccggtg aggtgaatac gggccagttc gacgatttgc
    10921 aggccttcgc cgaactctgt cgcaaccacc cgggtggcgc gtggctccac gtcgacgcgg
    10981 cattcggtct cttcgcagcg gcgtcgagcg accaccggca cctcctagcg ggcattgagc
    11041 acgccgactc ggtcggcgcc gacgcccaca agtggctcaa cgtcccctac ggctcagggt
    11101 ttgctttcgt tcgtgatgag gaggtcctgc gagccgcgtt cgccactacg ggcgccgcct
    11161 atctcgccgc ctcgggcggc tggggtgccg agagctacag ccccgagatg tcgcgacgcg
    11221 cccgcgcgat ccctgcgtgg tgcgcgctgc ggagtctcgg ccgcgagggg taccgggagc
    11281 tggtcgaccg ctgcatcggc aacgcgcgca agctggccga gtgggtcgac gccgagtccg
    11341 gcctggagct gatgaacgcc gaccgcatgc gcgagacgcc cttcatgatc gtctgcgtgc
    11401 gcttcaccaa cccgtcatgg gacgacgagg agcacgaccg gcgcaatcgc gagcggctcg
    11461 cttccctgca ggccgacggc cgcgtttacg ccagcacgac tgtgtgggat ggcaaggcgg
    11521 caatgcgctt cgccttcgac aactggatga ccaccgaggc ggacgtcgaa acgatctgcg
    11581 ctgtgcttcg cgagctgcga gatcagcaga ccggcgcctg atatgcgcgc ggcctcgctc
    11641 agcaagcatg cctagcaggc ccgcctgagg gtatcccatc aacccgcccc ttccggcccc
    11701 gcgccgtata cttcccgtag tgttagtgcg cccctgatgg cgtgccgccc gaagtgtcag
    11761 acgcgccaat gcgacgcttt gccacccgcc aagcccaagg gacctagcat gctcgacttc
    11821 atgagaaccg gctcgcttcg tgcgtccgtc gtgctccttg ccgcgctgat catggcgatg
    11881 atgccctcgg ccggtttctc gccggctgcc gcacaggatg cactcgcctg tcagccgatc
    11941 cccaaggcga ctccgaccgg aacgctgacg agagacgtcg aaactcccga attcgaggtg
    12001 ccggaggacg ccgtcccggt gacggtcggc tacacgccga tttccatctt cgcaccgatg
    12061 ttcgtcgcct acgagaaggg gtacttcgcc gagcagggcc tcgacgtgac gctcgaggcg
    12121 cttccgggcg ggactgacat ggtggtgcac gttgccaccg gcaacttcga cgccggcatc
    12181 ggcggtatcg ggccagccta ctggaacgcc gaggcacagg ggctgccggt gacggtcatc
    12241 gcgccgggcc acatggaaga agcgccggtt gcctcgccgc tgatgatctc aacggaagca
    12301 tgcgagagcg gcgagatcat gcaagtcagt gatcttgagg gcaagaaggt tgcggtcaat
    12361 gcaccgggcg cgaccgagat ctggctcgac gccgccctgc gcaccggcgg gctgacgatc
    12421 gaggacatcg atctccagta catgccgttc ccggacgccg tggctgcact cgacagcggc
    12481 gcgatcgccg cgtcaatcgt tggcgagccg ctcgccaccc aggctgagaa cgaaggcatt
    12541 gccgtgcgcc tcgcctcgga cttcgacgtc gccgactatc aggtaaccgc catcttcgcg
    12601 aacgacgagt tcctcgccga gcacccggag gctgcgaccg gtctcgtgac ggcttacctg
    12661 aaggccagtc gggatctgac cgaggatttc agcgacctac tccacctcgc gatcatcgaa
    12721 gagtacaccg gcgtaccggc ctcgctggtc gccgagtcgg tcaagccggt ctacgagctc
    12781 gacggtgaga tcgacctcga gtccctggca gatgtccagg agttcttccg caaccgcggc
    12841 ctgctcgagt acgaggagag catcgacccg gcctcgatgg tcgaccagac cttcgtcgat
    12901 cgagccatcg agaacattgg agagaaccag gcggactcat gaccgagatc gtacttccca
    12961 ccgtatcgaa tcgacgcttg cccactcgcg ccaacgaggc gatcgtctcg accacgcggg
    13021 tgggcaagac ctttgagacg ccgcgcggac cggtcagggc agtggaggac gtcaccctcg
    13081 cgttcgcgcc cggtagcttc aacgtcatcg tcgggccgag cggctgcggc aagtccaccc
    13141 tcctccgcat gattgccggg ctcgaatcgc cgacgagcgg ggacctctcc ttcaccagag
    13201 cgcaggagaa cgccagcgcg ccgaccaacg cactggtctt tcagggtcgc tccctcttcc
    13261 cttggctgaa cctccggcag aatgtcgcgt acggcctgcg gctgcaggga atatcacgtg
    13321 ctgagcgcag cgcgaaggcc gatgagctgc tgcatctggt cggtctgcgc aagttttcgc
    13381 gctcctaccc acatcaggtc tcggaaggca tgcggcagcg ggtggcgatt gcgcgggcac
    13441 tggccgtcga cccggacatc ctcctcatgg acgagccctt cggtgcgctc gacgagcaga
    13501 cccgcctcct gctgcaggag gagctgctgc gaatctggga ggcgaccggc aagacggtca
    13561 tctttgtcac gcactcgctc gacgaggcgc tgttgctggc tgacaccatc gtggtgatga
    13621 gcgcccagcc ggggacggtc cgtaccgtgt tccgggtgcc gttcccacga ccacgcacgc
    13681 tgaccgaggt tcgcaaggat ccgaaattcg ccgagctctt ctcaaggacg tgggaaatcc
    13741 tccgcacgga ggtgaacgcc gcgcgcgccc ggcaggagca ggagatcgcc tgatgagcct
    13801 cagacagcgg cgggccagcg aactcgccct cacgctgctc gccccgctcc tcctcctgtc
    13861 gatctgggag ctgctctccc gctccgagct gatcaacccg ctctactggc cggcgcccac
    13921 gagcctgtgg agcaccttcg ttgagctcct caccgagggc gatctactga ccgacatctg
    13981 gctcagcacg gtgcgcatcc tcggcgggtt cctgctcggg tcaattcccg ggatcgtgct
    14041 cgggctcgcc atgggcctct tctggcccgt gcgcgtcttc ctgatgccgc tggcggcggc
    14101 gctttatgcc atcccgaaga tcgcgatcct gccgctcgtg atgatcatct tcggcatcgg
    14161 tgaggcctcc aagttcatga tcgtcgcact ctcgatcttc tttctcgtcg tgctgaacac
    14221 catgagtggc gtgctggaga tcgaccggac cttccgggat gtcgcccgca acctaggagc
    14281 gtcgcggctg gagctcttca cgaccgtcgc cctgcccggc gcgctgccaa gcatcttcac
    14341 cgggctgcga ctggcaatgg gcttcgcgct gatcgtgatc gtcggcacgg agttcctgac
    14401 cgtctcgcag ggtggaatcg gcgcgatgat ttggcagagc tggacgattc tctcggtcaa
    14461 gaagatgatg gtcgggctga tcatcaccgg catcatgggg tggctgctct ccctgctcgt
    14521 ctcgtcggcg gagcgcctcg tgatgccctg gagggttgcc gactgatgag cacgacgacg
    14581 ccaacccccg ccgcgaccga ctgcacgacc aggcctcccc tctggaaggt ctggctggtg
    14641 gcggtgcggc cgttttcgtt cacgacctcc accgtaccgg tcattgccgc atcgatgctc
    14701 gcgctctatg agggcgccgt gaacgtctgg gccctgatcc tgatgctgat cgcctcgatc
    14761 tccacccacg cgggctgcaa cctcgccaac gactactatg atgacgagac cggcgtggac
    14821 aagaagcagg tgatcggcca ggccggcgtg ctgcaggcca actggctgac ccgccgggag
    14881 atcggtttcg gtattatcgt cgccttcgcc gtcgcgctgg tcgcggcgct cccgatcatg
    14941 atcgacatcg gccgcccgat cgtcatcctc gcgctgctca gcgccgcagc ggcctacttc
    15001 tatacgggcg gacctttccc gcttgcctac tgggcgctcg gcgaggtcac cgtcttcctg
    15061 gcaatgggtg tcggcatggt cgtcggcacc tactacgtgc ataccggtgc ggtcagcacg
    15121 atcgccgtgc tgctcagtgt cgcgatcggc tgtgtcgtga cggcgatcct ccacgcgaac
    15181 aacatccgcg accgcgaggt ggaccgccag caccgcaagc ggacgatcgc gaacctgctg
    15241 ccgcgtggct tcgccaacgc ggagttccag gtgctggtgc tggtgccgtt cgcgctgatt
    15301 gccgcgatga tcgtcctcga tccgggttcg tggcccatcg ccgtaagcgc gctggctctg
    15361 ccccgcgccc ttttcctggc gcgaacgatc gcggacgccg ataccccgcg tgaactgaat
    15421 ccctacgtgc gacagtccgc cggcctgcac atgcagttcg gcctgctcat caccgcaggc
    15481 tacatcgccg ctcgcctgat cgagatgcta tgaccttcag cttcaaatgg gccgccgcaa
    15541 tcgctcttgg tggactcgcc agcgtggggg ccgccgtggg ctactggtcc cgattcgtcc
    15601 atccgtatcg cacccaactc acgcacgaga tcatggccgt gccgcgcaag cacgcgcgcc
    15661 tcaacggtct gacgatcgcc ttcgtcagcg acacccacat cggcccgcac ttccgcaccg
    15721 acgacctgcg ccctgcgatt gagatgctgc gccgggccca gccggacatc ctcctcctcg
    15781 gtggcgacta cgtgtccgag tcaccgcgct tcatcgaccc gtccgtcgac gcgttgctgg
    15841 acatggcggc gactgcccgg ttcggcacct gggccatcct cggcaaccac gacctcgcca
    15901 acacaccgga acgggtggtg gaagcgctgg agcgcgcggg catcccgacg ttgatcaacg
    15961 acgctgccca cgccgagacg gatcgcggcg atctctggat cgtcggcatc gacgacgccc
    16021 tgctcggcga gccggatctc aaggcagcgt tcagcgggat ccccgccgac gcgccggtcg
    16081 tcgccatgtg gcacgagccg gacgttgccg aggacatgtt gcccttcgat ccgctcttca
    16141 tgctctccgg ccacacacat ggtggccaga ttcgcattcc cggcctggtg gatgccgctg
    16201 tgccgcggct gggcaagagg tatccgctcg gccgctacga ggttcagggc atgccgctct
    16261 acgtttccag cggggtcggc atgtatcgac cgccggtacg gttcaactgc ccacccgagg
    16321 tcgtgataat caccctgctc ggcgagcagg atggggcgga tatgctctcg tcgcagttgc
    16381 ctgcacgtgg cacactgcgt gcggaaatgt gaccgggagg atccggtcgc gtctcggact
    16441 cctcgcgcct ccggacctgg aggctctcac aggaaacagc tacgtgtcga acgaacagac
    16501 ggtccgcgtc ggcgtcatcg gttgtggcgc cggtatcttt cacctgaacg gctactcgaa
    16561 ggacccgcgc gtcaagatcg tcgcactcgc cgggctcgac accgaccgtt gcgagcggct
    16621 ggcgaaggac ttcgagatcc cgcgcgttta cacgcactat caggatctgc tcggcgacag
    16681 cgacatcgac gcagtaagcg tcgtggtgcc aaaccacctg cacctgccgg tcagtaaggc
    16741 ggcgttcgag gctggcaagc acgtgctggt cgagaagccg ctggccggaa cggtcgccga
    16801 cggtgagcag atggtcgcgc tggcgaagga gcacggcaaa ctgctcggca tcgcgttcca
    16861 gcgccgatgg cggcatgacg tgcagatcgt tagggagctc atcgagaacg gtgagatggg
    16921 cgacgtctac cacgccaagg cgttctggat gcgccgcagc ggcatccccg gctggggcag
    16981 ctggttcacc agcaaggagg cggccggcgg cggacccttg atcgatctcg gcgtgcacgt
    17041 gctcgatatg gcgctctaca tgctgggcaa cccgaagatc gtgagcgtct ccgctgcgac
    17101 ctatgacaag atcggcagcc agggcaaggg taactggccc ggcactccgg gtgcgaggac
    17161 gggtggctcc aacgcctacg aggtcgagga cttctcgact gccctcctcc ggttcaagga
    17221 cggcagcacc ctcgccctgg acactgcatg ggccgcctac acggagatga ccgatgagtt
    17281 cggcgtgcag gtcttcggca gtaacggcgg cgcgaagatc cactccaagg aatacgccga
    17341 tgtcggcacc ctgcagctct tcagcgatgt tgccggcacc gcgaccgacg cgaaaccgcg
    17401 cctgcagagc cgcaacggtc atgagtacat ctgccggcac ttcgtcgacg ccgtcttgaa
    17461 cggaacaccg gtttcgccga gcggcgagga aggactcgat cgggtacgga tcatcgaggc
    17521 aatctaccgc tcggcggcac ttggtcgcga ggtgctgatc gacgaggtca tcgagcctgt
    17581 ccaggacgcc gccgactgag atcgggaggg cgaccaggaa gatcgcccgt gcgggttgac
    17641 atggagtcga ccctcaggga aggatggata catgccaagc gagattcgcg ttaccgtatg
    17701 gaacgagttc cgccacgaga agaaggaccc ggaggtcggc gaggtctacc ccgacggcat
    17761 ccacggcgcc atcgccgaag ggctcaacga ggcccaaggc atcaccgccc gcaccgcaac
    17821 actcgacgag ccggagcacg gtctgcccca ggacgtactc gacaataccg atgtcctcac
    17881 ctggtggggt cacatggccc accgcgaggt cgatgacgcg gtcgtcgagc gcgtccacga
    17941 gcgcgtgatg gcgggcatgg gcctgatcgc gctacattcg gcccactttt ccaagatctt
    18001 caagaagctt atgggcacga cctgcgacct caagtggcgc gaagccgatg agaaggagcg
    18061 gctgtgggtg gtccggccgg ggcacccgat cgtcgagggc atcggcgagt acatcgagct
    18121 cgacaaggag gagatgtacg gcgagcactt cgacatcccg cagccggacg aactcatctt
    18181 cgtcagctgg ttccagggtg gcgaggtctt ccgtagcggt gccacctaca accgcggcgc
    18241 cggcaaggtc ttctactttc gtcccgggca cgagacctac cggaccttct acaaccctga
    18301 gatacggcgt gtcatcgcca acgccgtccg ttgggcagca ccggtcgagc gccctgctcg
    18361 aaccttcggc aacagtgatc cgatcgagga tctgccgtgg tacgacaagc agcacgccgt
    18421 caccaagggc taccggtagc gatcagcccg gac
//
LOCUS       DASYWL010000118         6659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_117968, whole genome shotgun sequence.
ACCESSION   DASYWL010000118 DASYWL010000000
VERSION     DASYWL010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6659
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117968"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VGR22_04280"
     CDS             complement(<1..515)
                     /locus_tag="VGR22_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127819.1"
                     /translation="MGLARLAIHLFTLSVAVALSIGGAAGAVGADEADSAAGLIVDTG
                     EGEPIHVVVTFAGQEITALDALQAAELGLVTVEFGGLGAAVCEIADTGCDPSTCRQRV
                     CQTGNPDSPFWQYWEQDDSGAWALSPLGASRARLDDGDIAAWVWTGPVPDLPSMTWES
                     LAASAGAPTSVT"
     gene            complement(517..1548)
                     /locus_tag="VGR22_04285"
     CDS             complement(517..1548)
                     /locus_tag="VGR22_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEV2127820.1"
                     /translation="MTSLTVGLIGALLLTVTVSGTAAQPATPTGVVAEADLDAAVMWL
                     IAQQLEDGSFPGFSGESDPSMTIDAVLALIAAGLRGEDTAGAVASAMDYLTSGDIAGA
                     YAETGVGQAAKLILTAVATGGDPRDVGGVDALELVVEGQNPETGMYGASIFEHGLALM
                     ALAATGTEVPASAIEALEATQGPEGGWAFDTSTEEGAADSNTTAMVVQALVAIGEGES
                     EMAQEAIAFLEETIESEEGALYQPGAAFPPDANSTALVLQAAIAAEQDLESDPWAALQ
                     SALAGFQNEDGAFHYSPDDTADNLFATVQAIPAVAGVAFPIVPADSEAIPVASPVTLV
                     PHGMIVRAA"
     regulatory      complement(1744..1869)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2063..2911
                     /locus_tag="VGR22_04290"
     CDS             2063..2911
                     /locus_tag="VGR22_04290"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV2127821.1"
                     /translation="MGILETIALGGAAAAAAILGSMLGLGGGVFLVPIFTLFFGVDAK
                     IAIAASAVSVITNSVVGSTVHLRNAFTNIHLAMLLGITTVTGAIAGALLAIVVDASVL
                     YFIFGVVLLAAAISMLVRRQKPIPNSEPEVPDPFLLRATYTDRATGGAVSYVPRFVKA
                     GLAASTFAGVLSGMLGVGGGVIQVPMMNLLMRVPVKAAAGTSAFLVGITAVATATVFY
                     ADGKVDATVVVPAMIGIFLGSKLGSNLTSRLKTANLVVLFVAIMAYLSISMILRAFGI
                     TLPGQE"
     gene            2916..3284
                     /locus_tag="VGR22_04295"
     CDS             2916..3284
                     /locus_tag="VGR22_04295"
                     /inference="COORDINATES: protein motif:HMM:NF019459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HEV2127822.1"
                     /translation="MDQSQSAPPPEAQVALNEPTARVLTWGFRISAALLLIGIVVSAV
                     QGEELHESVADIPTIIEQILDGNGAGIVALGILVMIATPIAATLSVVLSCLRIGDRRY
                     AIIASAVLIILFISAASSAL"
     gene            3298..4086
                     /locus_tag="VGR22_04300"
     CDS             3298..4086
                     /locus_tag="VGR22_04300"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEV2127823.1"
                     /translation="MIPAIDRLAVIVAMPSELQHLPGLPHVPEYGDTLWPSVAWQQGA
                     LEIVAVLSGIGMIRAAAATEATIDQQRPDAVLNFGCTGAHQADVHVGDVIMSTRSVAH
                     ATMVIEPGGMARFDLHDGMEAPPPGATGLPSDPSLLSIARAAAEGWMPDPWVAATGHN
                     AHPQVLEGVVASADVWTQAPEAISALVDRHQSLCEDMEAAAIATICSLHGVPFLTIKD
                     ISNNELQQATIFDPGMAHLPEGEVGRRAAALTGRLIERIVSAES"
     gene            complement(4415..5122)
                     /gene="rimM"
                     /locus_tag="VGR22_04305"
     CDS             complement(4415..5122)
                     /gene="rimM"
                     /locus_tag="VGR22_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEV2127824.1"
                     /translation="MPDSRRSQSPSGDAGAPSSAALASSRSPRRRRGSSGAPKRLIDM
                     QGPDPDAKVEDIRLTVGMLVGSHGVQGELRMTLQTDAPEHLLEIERVYLGDSDTPTAL
                     EGVRFHGDSALIFLEDVETPEEAKALRGTPVRIAGSDARPLEEGEYFYYQLVGLRAVT
                     PEGDTLGVVVDMIETGAHDVLVIAPEGSRASRSPASEVLIPHHESYVHEVDPKAGTIV
                     VTKPVYSDEVNQQQGCE"
     gene            complement(5115..5420)
                     /locus_tag="VGR22_04310"
     CDS             complement(5115..5420)
                     /locus_tag="VGR22_04310"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEV2127825.1"
                     /translation="MADGAVVHQRSDDAAVAALTDLVIWLTGELVDDADQAIISTDRR
                     GNHVAVRLELPEQQLGQVIGRGGRIARALRTALMVAGSRHDLRVSLDIEATDEDSDA"
     gene            complement(5423..5704)
                     /gene="rpsP"
                     /locus_tag="VGR22_04315"
     CDS             complement(5423..5704)
                     /gene="rpsP"
                     /locus_tag="VGR22_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEV2127826.1"
                     /translation="MIKLRLRRMGAKKRPHYRIVATEHSSPRDGRFIESIGHYNPIAD
                     PPVIKVDLERARHWIGNGAKPSDTVAALIRKAEAATAEAPVDETAKAEA"
     gene            complement(6477..>6659)
                     /locus_tag="VGR22_04320"
     CDS             complement(6477..>6659)
                     /locus_tag="VGR22_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127827.1"
                     /translation="LTDVVMRRTMVGYARGAGLDAAPAIAQVAVESGYWSEREATEQV
                     DEFRRYLARFLPHNMN"
BASE COUNT         1126 a         2345 c         2051 g         1137 t
ORIGIN      
        1 gtcacggacg tcggcgcgcc agcgctcgcc gccagcgact cccatgtcat cgacggcagg
       61 tccggcacgg ggccggtcca aacccaagcc gcgatatcgc catcatcaag cctcgcacgg
      121 ctggcgccga gcggactcag cgcccacgcc ccgctgtcgt cctgctccca gtattgccag
      181 aatggcgagt caggatttcc cgtctggcac acccgctgtc gacacgtgct tgggtcacat
      241 ccggtatcgg cgatctcgca caccgccgcg ccgaggccgc cgaactcgac cgtgaccagg
      301 ccgagctcgg cggcctgaag cgcatccagc gccgttatct cctgtccggc gaacgtgacg
      361 acgacatgga tcggctcgcc ctcgccggtg tcaacgatca gtcccgcagc ggagtccgcc
      421 tcgtcggcgc ccactgctcc cgccgcacca ccgatggaga gcgccaccgc gacggagagc
      481 gtgaagagat ggatcgccag ccgggccagt cccatctcag gctgctcgca cgatcatccc
      541 gtgcggcacg agggtcacgg gcgaggcaac cgggatagcc tcgctgtcgg caggaacgat
      601 cggaaaggca acaccagcca cggccgggat cgcctgcaca gtggcgaaga ggttgtccgc
      661 ggtgtcatcc gggctgtagt ggaaagcacc atcctcgttc tggaaccctg ccagggcgga
      721 ttgcagagcg gcccagggat ccgactcgag atcctgctcg gcggcaatcg ctgcctgcaa
      781 caccagcgcc gtcgagttgg cgtccggcgg gaaggccgct cccggctggt agagcgcccc
      841 ctcctcgctc tcgatggtct cttcgagaaa ggcaatcgcc tcctgtgcca tctcggattc
      901 gccctcgcca atcgcgacca gcgcctgcac aaccatcgcg gtcgtgttgc tgtcagcggc
      961 gccctcctcg gttgaggtgt cgaacgccca accgccttcg ggtccctgcg tcgcctccag
     1021 cgcctcgatc gcagaggccg gcacctcggt gccagttgca gcgagcgcca tcagggcgag
     1081 cccatgctcg aaaatcgagg ccccgtacat gcccgtctcc gggttctgtc cctcgaccac
     1141 caactcgagg gcgtcgacac cgccgacgtc ccgcgggtcg ccacctgtcg cgaccgccgt
     1201 caggatgagc ttggcggcct gccccacccc ggtctcggcg taagcacccg cgatgtcacc
     1261 tgacgtcagg tagtccatcg ccgaggcaac cgcccccgcc gtatcctcgc cacgcagccc
     1321 ggcggcgatc agcgccagca ccgcgtcgat ggtcatcgac ggatcactct cccccgaaaa
     1381 accggggaag cttccgtcct cgagctgctg tgcgatcagc cacatgaccg ccgcatccaa
     1441 atccgcctcg gcgacgacgc cggtcggggt cgccggttgc gccgccgtgc cggatacggt
     1501 caccgtcagc agcagcgccc cgatcagccc gactgtcaga ctcgtcactc gcttcatcgc
     1561 ctgccctcac ttctcctgcg cccgtgtcat cgcgcacggc cgccgggcac cgtgccacca
     1621 ccttcacgaa atgcaacacc ccctgactct gggcagagtc agggggtgag gacttccgcg
     1681 atcggtccgc caatcgatgg tccgtgcgtc acgtcgggtc accgtcccct gttcggcgca
     1741 gggtcagcga caatctccgg aggcgggtgc tctggcttcg gccgcaacgg ccgttacagt
     1801 tgcgcgacag cgccggtctc acaccggact tcccccgctc gacgaagcgc cccattctca
     1861 acgtggcgcc tcgtgaagct ccccggatct cgtctttcgg ttgcgaaggt gcttcccgac
     1921 atcatgccgg gtctccagca tcatcccacc cgctcaccac aactgtcaac gacacggatg
     1981 cggagcggcg tagcgtagta taaggggcgg ggccacctga gcggccagct tcaaacgcca
     2041 ctccgggaag gaagagacga ctgtgggaat cctggagacg atcgcgctcg gcggcgctgc
     2101 cgctgccgct gcgattctcg ggagcatgct cgggctcggc ggcggggtct ttctcgtgcc
     2161 gatcttcacg ctctttttcg gtgtcgacgc gaagatcgcc atcgcggcca gcgccgtctc
     2221 ggtgatcacc aactcggtag tcggctcgac ggtccacctg cgcaacgcgt tcaccaacat
     2281 ccacctcgcg atgctgctcg gcatcaccac ggtaaccggc gcgatcgccg gtgctctgct
     2341 cgcgatcgtg gtcgacgcca gcgtgctcta cttcatcttc ggtgtcgtgc tgctcgcggc
     2401 agcgatcagc atgctcgtcc gtcgccaaaa acccataccg aacagcgaac cggaggtgcc
     2461 ggaccccttc ctccttcgcg caacctacac cgaccgcgca acgggtgggg cggtgagcta
     2521 tgtgccccgg ttcgtcaagg cggggctcgc cgccagcacg ttcgccggcg tcctctccgg
     2581 gatgctgggt gtcggcggcg gggtgatcca ggtgccgatg atgaacctgc tcatgcgggt
     2641 accggtcaag gccgcggcgg gcaccagcgc tttcctggtc ggcatcaccg ccgtcgccac
     2701 cgcaaccgtc ttctacgctg acggcaaggt cgatgcaacg gtcgtcgttc cggcgatgat
     2761 cggcattttt ctcggctcga agctcggctc gaacctcacc agccgcctga agaccgccaa
     2821 cctcgtggtg ctcttcgtgg caatcatggc ctatctgtca atctcgatga tcctcagggc
     2881 cttcggcatc acgctgcccg gacaggagta acgccatgga ccagtcgcag agtgctccac
     2941 cacccgaggc gcaggttgcc cttaacgagc caactgcccg cgtgctgacc tggggtttcc
     3001 gcatctcggc ggcgctgctg ctgatcggca tcgttgtctc agccgttcag ggcgaggagt
     3061 tgcacgagtc ggttgcggac atccccacga tcatcgagca gatcctcgac ggcaacggcg
     3121 cgggcatcgt cgcgctcggc atcctcgtga tgatcgccac gccgatcgct gcgacactga
     3181 gcgtggtcct ctcctgcctg cgcatcggcg accgtcgtta cgcgatcatt gcctctgccg
     3241 tgctgatcat cctcttcatc tccgccgctt catccgccct ctgatcggaa gccgttgatg
     3301 atccctgcca tcgaccggct cgccgtgatc gtcgccatgc cgtcggagct gcagcacctt
     3361 cccggcctgc cgcacgtgcc ggagtacggg gacacgctat ggccgtctgt cgcgtggcag
     3421 cagggagcac tggagattgt cgccgtgctg agcggcatcg gcatgattcg tgctgctgcg
     3481 gcgacggagg caaccatcga ccagcaacga cccgacgccg tcctcaactt cggctgcaca
     3541 ggcgcccacc aagcggatgt ccacgtcggt gacgtcataa tgagcacgcg ctcggtcgcg
     3601 cacgccacga tggtgatcga accgggaggc atggctcgat tcgacctcca cgacggcatg
     3661 gaagccccgc cgccgggcgc gaccggcctc ccctccgacc catcgctcct gagcatcgca
     3721 agggcggcgg ccgaggggtg gatgcctgat ccgtgggttg ccgccaccgg ccacaatgca
     3781 cacccgcagg tgctggaagg cgtggtcgcg tcggcggatg tctggaccca ggctcccgag
     3841 gccatatcgg ccctggtcga ccgccatcag tcactctgcg aggacatgga ggcggccgcg
     3901 atcgccacca tctgttcgct gcacggtgtc cccttcctga ccatcaagga catctcgaac
     3961 aacgagctgc aacaggcgac gatcttcgac ccgggaatgg cgcacctgcc cgagggcgag
     4021 gtcggccgcc gggcggcagc tctcaccggg cgactgatcg agcgaattgt ctcggccgag
     4081 agttgatcgg ccgcctgcgg aaaaaaatct caaggctgcc tgctggtcga cggaatcatg
     4141 cgacacttgg acgatacgtg acgcggactc cgggggaact gccggtgcac cctgggaacc
     4201 gtcggggcgg attccgcggg catgagtcca gcgctcgaag gcaagcagaa gcccgaagcg
     4261 cgaaaccgcc cagccaacct gctgggcggg gaatgctttg cgctccggat gacacgccat
     4321 ggagcaggtt gccgtgacgg tgacacgcag gggactgatc tgcacagcag tggcgggcca
     4381 ctgccaacct ggaaaccagt gcgtgggcga cccgctactc gcagccctgt tgctgattca
     4441 cctcgtcgga gtacaccggc ttcgtgacga cgatcgtccc tgccttgggg tcgacctcat
     4501 ggacgtagga ctcgtggtga gggatgagca cctcgctggc tggcgaacgg ctcgcgcggc
     4561 tgccctccgg cgcaatcacc agcacgtcgt gggcgccggt ctcgatcatg tcgacgacca
     4621 cgcccaacgt atcgccctcg ggagtgaccg ccctcaaccc gacgagctgg tagtaaaaat
     4681 actcgccctc ttcgagcggc cgggcatcgg acccggcgat ccgcaccggc gtgccacgca
     4741 gcgccttcgc ctcctccggc gtttcgacgt cctcgagaaa gatcagtgcg ctgtctccgt
     4801 ggaaccgaac cccctccaga gcggtgggcg tgtcactgtc gccgaggtag acccgttcga
     4861 tctcgagcag atgctcggga gcgtcggtct gcagcgtcat gcggagctct ccctgcacgc
     4921 cgtgggagcc gacaagcatg ccaacagtga gacggatatc ctcgaccttc gcatcagggt
     4981 ccggcccctg catgtcaatg aggcgtttcg gcgcaccgct actgcctcgc cggcggcgcg
     5041 gcgaacgcga cgacgccaga gcagccgagc tcggcgcccc ggcgtctcct gacggtgact
     5101 gggacctccg ggaatcaggc atcgctgtcc tcgtcggtcg cttcgatgtc gaggctcacc
     5161 cggagatcgt gccgggaacc ggcaaccatc agcgccgtcc gcagcgcacg ggcgatccgc
     5221 cctccacgac cgatcacctg tcccagctgc tgctcgggca gttcgagccg gacagcgacg
     5281 tggttgccac gccgatcggt ggagatgatc gcttggtccg cgtcgtcgac cagctcgccg
     5341 gtcagccaaa tgaccagatc ggtcagcgcg gcaaccgccg cgtcgtcgga tcgctggtgt
     5401 acgacagctc cgtccgccac gactaggcct cggccttggc cgtttcatcc acaggcgcct
     5461 cggccgtggc agcctcggcc ttgcgaatca gagccgccac cgtgtcggac ggcttggcgc
     5521 cattgccaat ccagtggcgg gcacgctcaa gatcgacctt gatgacgggt ggatcggcaa
     5581 tcgggttgta gtggccgatg gactcgatga aacgaccatc gcgcggcgag ctgtgctcag
     5641 tggcgacgat gcggtagtgc gggcgcttct tcgcgcccat ccggcgcaga cgcagcttga
     5701 tcacgtggcg ttactccgat ctggttgtcg tcgcgggcca gaacacgccc cgctggttct
     5761 ctgcaaaatc aaagatcagg gtggacccga gcggtgctcg gtgtcacgag agcccgtagc
     5821 catcgcgggg aggacgagga ggcactgaac aacctctcca gggtgtccgc aaatcacccc
     5881 tacccggtct cgggcagccc cgcctcggaa tcgacatggg catccgtaac agcggacacg
     5941 ccgatgccgc ccgtatcaca gcggacccac catcaaaccc tagcacagtc ggcagaaaat
     6001 ggcgaaggcc tcgctacgac gcggccttgc gcgcatcgct ggtatcggtg acgtgctctc
     6061 tgccatctcc cgcgctaccc gagcaggggc agcaggccag gagcacatcg gtgaggccac
     6121 gggagaggcc catggaccgg gctcgctcaa caaacaccgt tcggtcatgt ccctcgacca
     6181 atgcctcggc gatgaggcga ctcggacgtg cctctgacga ggtgtattcc ggtccggcta
     6241 ccatcaatcc cgtcatcctt tccggtcgac gcgcgcaaca acatcagggg gcatgcccgc
     6301 ctgctcctcc gcgcatctcc aactactctc aggataagtg tgaagttccg gccagtcatc
     6361 cggattgcac cgtcagccta gcaaatcgcg cccgctctgg caatagtcct ttgtgactat
     6421 cgtgcagcat gtctccacca tcggacgcac cctcaatagc accaaggcgg tcgttatcag
     6481 ttcatgttat gtggcaagaa ccgtgccagg tatcgtcgga attcgtcgac ctgctcggtt
     6541 gcctcgcgct cgctccagta gccgctctcc acagcgacct gggcgattgc cggggccgcg
     6601 tccagcccgg ctccacgcgc atagccgacc atcgtgcggc gcatcacaac gtccgtcag
//
LOCUS       DASYWL010000119         3111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_305625, whole genome shotgun sequence.
ACCESSION   DASYWL010000119 DASYWL010000000
VERSION     DASYWL010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305625"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VGR22_04325"
     CDS             <1..600
                     /locus_tag="VGR22_04325"
                     /inference="COORDINATES: protein motif:HMM:NF024819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside adenylyltransferase
                     domain-containing protein"
                     /protein_id="HEV2127828.1"
                     /translation="DGLVLTEADLEAGPDGIAGERPVIFDNYARLGPDGSGRNPVTWQ
                     MLRQCGITYRGSSLEPARLWHDPDRLDSWTRENLLRYWRPWLTQTEPLAARPDLDSLI
                     AEATEWGVLGVTRLHYTLATSAITSKHGAGLYALETFSSAWHPIIEEALRIRERRDQP
                     SRYGNPIERAHQVRDYIAMVINDALTLPVCDAAADKPQR"
     gene            624..1562
                     /locus_tag="VGR22_04330"
     CDS             624..1562
                     /locus_tag="VGR22_04330"
                     /inference="COORDINATES: protein motif:HMM:TIGR03621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03621 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEV2127829.1"
                     /translation="MAQDRRPFRFGVQISKARPVSEWRERAREAEALGYNIFLMPDHL
                     GPQFAIGPALAVVAEAAPALRIGTLVWQNDWRHPTLLAKEAYTLDVLSEGRFELGIGA
                     GGSFPPEFDWMGVPLAPAGERVGRLEECIRILKAVAGPDPVTFSGKHHTLTEYEGHPK
                     PVQRPYPPIHVGAGGPWMLGIAAREADIISLLPMLGQGAGIDEGELGPEGFDRKIALV
                     REVAGERFPGIELNILIQGLAVTDDRDTGIARLREERGFDSDAWFDSPMVFYGSRDHI
                     TETMLANQERFGVSYYAVFEHQLEEFAPIVARLAGH"
     gene            complement(1633..2307)
                     /locus_tag="VGR22_04335"
     CDS             complement(1633..2307)
                     /locus_tag="VGR22_04335"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV2127830.1"
                     /translation="MPEPNGHHPESTTLTLVRHGETDWNAQRRMQGRADIPLNEKGRK
                     QAELVAQRLEGTQWDGIVSSPLVRVIEVSRAIARAIDIPEHEIIERHDLVERNFGEAE
                     GMTLEERTERFGVDGHIPGIETYEELDHRVMRALREIEAQYRSKRVLVITHGGVITGT
                     LQQLTDGELGHGKVFIANVSLTTIKVQDGEWHVEMMNDYSHLLGTEVEPITTPVTGSG
                     QASVKR"
     gene            complement(2498..>3111)
                     /locus_tag="VGR22_04340"
     CDS             complement(2498..>3111)
                     /locus_tag="VGR22_04340"
                     /inference="COORDINATES: protein motif:HMM:NF039902.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEV2127831.1"
                     /translation="DLLEHFTLERVSASPATFDRNKLDWMNQHWINHLLTVEELATRC
                     LPFLMEAGTIEQGPADESHPRFEDVKTAAALLKDRIKFLTEAPDLMSYFLQDELADYD
                     ASLLIPKKADAATAQRALEAVAGVLPEVDLKDEEATEARLRALADELGLKAGQLFMPI
                     RVAVTGRTQSPGLFETLRAIGQERVERRIAHAIDKLKALPAEG"
BASE COUNT          492 a         1043 c         1035 g          541 t
ORIGIN      
        1 gacggcttgg tgctcaccga ggcggacctc gaagccggac cggacggcat cgcgggcgag
       61 cgcccggtca tcttcgacaa ctacgcccgg ctgggcccgg acggcagcgg ccgcaacccg
      121 gtgacgtggc agatgctgcg ccagtgcggc atcacctacc gagggtcatc cctcgagcca
      181 gcgcgcctct ggcacgaccc cgaccggctc gattcctgga ctcgtgagaa tctgctgagg
      241 tactggcgac cgtggctgac gcagaccgaa cccctcgccg cacggccgga cctggattcc
      301 ctgatcgcag aggcaaccga gtggggcgtg ctcggcgtga cacggctgca ctacacactg
      361 gcgaccagcg cgatcacctc gaagcacggt gccggcctct acgcgctgga gaccttctcg
      421 tccgcgtggc acccgatcat tgaggaggcg ctgcgcatcc gcgagcggcg ggaccaacca
      481 tcgcgctacg ggaacccgat cgagcgagcc caccaggtgc gcgactacat cgccatggtg
      541 atcaacgacg cgctgacgct gccagtatgc gatgctgccg ctgataagcc ccagcgatga
      601 ccacatcaga tggaggcaga ttgatggccc aggaccggcg cccgttccgc ttcggcgtcc
      661 agatctcgaa ggcccgtccg gtgagcgagt ggcgcgagcg cgcccgcgag gccgaggcgc
      721 tcggctacaa tatcttcctg atgccggacc acctcggccc ccagttcgcc atcggaccgg
      781 cgctggccgt ggtcgccgag gcggcgcccg ccctccggat cggcacgctc gtctggcaga
      841 acgactggcg ccacccgacg ctgctggcca aggaggcata cacgctcgat gtcctctcgg
      901 agggccggtt cgagctcggc atcggcgcgg gcgggtcgtt cccgccggag ttcgactgga
      961 tgggcgtgcc gctggcgccc gccggggagc gcgtcggccg gctcgaggag tgcattcgca
     1021 tcctcaaggc ggtggccggt cctgaccctg tcaccttctc gggcaagcac cacacgctga
     1081 ccgagtacga ggggcatccg aagccagtgc agcgccctta cccgccgatc cacgtcggcg
     1141 cgggcggccc gtggatgctc gggatcgccg cccgcgaggc ggatatcatc agcctgcttc
     1201 cgatgctcgg gcagggtgca gggatcgacg agggggagct cggcccggag gggttcgacc
     1261 ggaagatcgc gctggtgcgg gaggtcgccg gcgagcggtt cccggggatc gagctcaaca
     1321 tcctcatcca gggtctggcg gttaccgacg accgggacac cgggatcgca cgattgcgcg
     1381 aggagcgagg cttcgacagc gacgcctggt tcgactcccc gatggtgttt tacggcagca
     1441 gggaccacat caccgagacg atgctggcga accaggaacg gttcggtgtc tcctactacg
     1501 cggtcttcga gcatcagctg gaggagttcg ccccgatcgt cgcgcgcctc gccggccact
     1561 gagcgatcag ccgcaacatc ctgattggga agaagggcgg acatagagtc catccgtacg
     1621 ggtcttggtc ggctaccgct tgacgctcgc ttggccagag ccggtgaccg gtgtggtgat
     1681 cggctcgact tcggtgccga gcaggtgcga gtagtcgttc atcatctcga catgccactc
     1741 gccatcctgg accttgatcg tcgtcaggct gacgttggcg atgaagacct tcccgtgccc
     1801 cagctcgcca tcggtgagtt gctgcagcgt accagtgatg acaccaccgt gcgtgatcac
     1861 cagcacccgt tttgagcgat actgcgcctc gatctcgcgg agcgcacgca tcacacgatg
     1921 gtccagctcc tcgtacgtct caatgccggg aatgtggccg tcaacgccga agcgctcggt
     1981 gcgctcctcc agcgtcatgc cctcggcttc gccgaagttg cgctcgacga ggtcgtgccg
     2041 ctcgatgatc tcatgctcgg gaatgtcgat tgcccgggcg attgcgcggg agacctctat
     2101 cacccgcacc agcgggctgc tgacgatgcc gtcccactgc gtgccttcca gccgctgggc
     2161 gaccaactcg gcctgcttgc gacccttctc gttgaggggg atgtcggcgc gaccctgcat
     2221 gcgccgctgg gcgttccagt cggtctcgcc gtggcggacc aaggtcaggg tcgtcgattc
     2281 ggggtggtgg ccgttgggtt ctggcatgta gcccgttgtc ctcgttctgt gaaagccaat
     2341 ccgtcatttc gaccagagcg cgagcacaag cgagcgcgga gtggagaaat cccatgctgg
     2401 ccatagagtt acccggccgc aagagatttc tccacgcggc ccttcgggcc ttgatcgaaa
     2461 tgacaggttg aagacgacgc agattgtcgt cagtccccta cccctcggcc ggcagggcct
     2521 tgagcttgtc gatcgcgtgg gcgatgcggc gctcgacgcg ctcctggccg atggcgcgga
     2581 gggtctcgaa gagtcctggc gactgcgtgc gaccggtcac ggcgacgcgg atcggcatga
     2641 agagctgccc cgccttcagg cccagctcgt cagcgagtgc ccgcagccgt gcctcggtcg
     2701 cctcctcgtc cttgagatcg acctccggca gcactccggc aaccgcctcc agcgcccgct
     2761 gtgcggtggc ggcatccgcc ttctttggga tcagcaggct cgcgtcgtag tcggccagct
     2821 cgtcctgcag gaagtagctc atcaggtccg gcgcctcggt caggaacttg atccgatcct
     2881 tgagcagcgc cgccgccgtc ttcacgtcct cgaatcgcgg gtggctctcg tccgccggcc
     2941 cctgctcgat cgtcccggcc tccatcagga agggcaggca gcgcgtggcg agctcctcga
     3001 cggtgagcag atggttgatc cagtgctgat tcatccagtc gagcttgttt cggtcgaagg
     3061 tcgccggcga tgcgctcacc cgctcgaggg tgaagtgctc cagcaggtcg t
//
LOCUS       DASYWL010000120         3015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_286793, whole genome shotgun sequence.
ACCESSION   DASYWL010000120 DASYWL010000000
VERSION     DASYWL010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286793"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="VGR22_04345"
     CDS             complement(<1..818)
                     /locus_tag="VGR22_04345"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2127832.1"
                     /translation="MQNGHKPDSTAPSTIIMGAGPAGLCSAYVLSKAGYPATVVEAAP
                     FVGGLARTIKRQTDHGEFKFDIGGHRWFTKNDELNDLFREVVGEELLWVNRISRIYFD
                     GKFVDYPLKLANALKAVGPVTAARAVFDYGLIQARRKAAPREVVSMEDAYVAQYGRTL
                     YELFFQAYSEKVWGMRCSELSGDWVSQRTKGMSLVTAIKDAVVPSKGKVVSLIDEFMY
                     PRYGFGRFSERMADAIEAAGNEIKLKSPVEKIVLEGNRVKGVQVRTEEGSTLIEG"
     gene            complement(948..1373)
                     /locus_tag="VGR22_04350"
     CDS             complement(948..1373)
                     /locus_tag="VGR22_04350"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEV2127833.1"
                     /translation="MISVESWPQPAVRLWVLAQRFRKFLTVGAVGLLVNQAVLAMLHD
                     GLAVAVVAASPFAIFLSMIVTFALNEHWTWHDRGTGRVISRAMTYVPINLGGLLINWS
                     VLYYLHEEHAVHYLAANLIGAGTAAIWNFLLNHSITWRR"
     gene            complement(1544..2842)
                     /locus_tag="VGR22_04355"
     CDS             complement(1544..2842)
                     /locus_tag="VGR22_04355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEV2127834.1"
                     /translation="MTRSRIHGRDGSSQANRRPPVSRWLSLSLLIALAITFGGMLGAR
                     FSADNANASTMLTQIPEFQVIEETYDLIRENYVLSEEFSDEELMYGAATGMVEALGDE
                     GHSTFLTPVEADEFERSSRGELIGIGIQVDTTGPQPVVVAPIDGSPAFEAGIRSGDVI
                     LAVDGVQSTEVDPEELVDLIRGEAGTDVTLTLRHQDEAEPYTVTITRARIEIDPVSSL
                     MMPNGVLWLRVSQFSAGATEGVVEALREGKAQGMTGVILDLRNNPGGLVFEAIGIGSQ
                     FLPGGSVLYQEQNADGSVHEVTTIGNSGEWQEGDLVVLINEGSASASEIVSSGLRDND
                     RAELYGETTFGTGTVLLPFELSGGSVALLGTKLWLTADGEQIWKRGVEPTTVISLEPG
                     VSPKLPIEFETDSVSREQLQSLEDTQLLSAYRELVPNASS"
BASE COUNT          542 a          964 c          984 g          525 t
ORIGIN      
        1 ccctcgatca gcgtgctccc ctcctcggtg cgcacctgca cgcccttgac ccggttgccc
       61 tcgagcacga tcttctcgac cgggctcttg agcttgatct cgttgccggc cgcctcgatg
      121 gcgtccgcca tgcgctcgga gaagcggccg aagccgtagc gcggatacat gaactcgtcg
      181 atcaggctga cgaccttgcc cttggaggga accaccgcgt ccttgatggc cgtgacgagc
      241 gacatgccct tggtgcgctg gctcacccag tcgcccgaga gctcgctgca cctcatgccc
      301 cagaccttct cgctgtaggc ctggaagaag agctcataga gtgtccggcc gtactgcgcg
      361 acgtaggcgt cctccatgga caccacttcc ctgggggccg ccttgcgcct ggcctggatg
      421 aggccgtagt cgaagacggc gcgggccgcg gtaaccggtc cgaccgcctt gagcgcgttc
      481 gcgagcttga gcgggtagtc gacgaacttg ccgtcgaagt agatgcggct gatgcgattg
      541 acccacagga gctcctcgcc gaccacctcg cggaacaggt cgttgagctc gtcgttcttg
      601 gtgaaccagc ggtggccgcc gatatcgaac ttgaactcgc cgtggtcggt ctggcgcttg
      661 atcgtgcggg caaggccacc gacgaaggga gccgcctcca cgacggtggc gggataaccc
      721 gccttgctga gcacataggc cgagcagagg ccggccggac ccgctcccat gatgatcgtg
      781 gacggggccg tggaatcggg tttgtgaccg ttctgcatcg agcgcgttcc ttcccttcgt
      841 tgcgtctccg gggccattca ctatggcgtt gcacaccaga tggcgttgca caccagccgc
      901 aggccggctg gcaacccggt gtcagatgct ctaaggcgaa ccggggctta ccggcgccac
      961 gtgatggagt gattcagcag gaaattccag atcgccgcag tgccggcgcc gatcaggttc
     1021 gccgccaggt aatgcacggc atgttcctcg tgcagatagt acagcacgct ccagttgatc
     1081 agcaggccgc cgaggttgat cggcacgtag gtcatcgccc gggagatgac ccgcccagtc
     1141 ccacggtcgt gccatgtcca gtgctcgttc aacgcgaagg taacgatcat ggagaggaag
     1201 atggcaaagg gcgacgctgc gaccacggcg acggcaagcc cgtcatgaag catcgcgagg
     1261 acagcctggt tgaccagcaa gcctaccgcg ccgactgtca gaaatttcct gaagcgctgg
     1321 gcaaggaccc atagccggac cgcgggttgt ggccatgatt caaccgagat catccctgct
     1381 cccagtgttc gagcgtgctg tcgagaaaga acgcgcgtcc aatccccctc gtacgaggga
     1441 gctcgctgtc caaatggcgc acgcataaac gataccatgg caaacaggcg cccgcgcagt
     1501 gcacgtacgg gcgcctgttg ttaccagaag cgtgccggat ggatcaggaa gacgcgtttg
     1561 ggaccagctc ccggtacgcg gagagcaact gcgtgtcctc cagcgactgg agctgctcac
     1621 gggagacact gtcagtctcg aactcgatcg ggagcttcgg cgagacacct ggctcgaggc
     1681 tgatgaccgt ggtcggctcg acgccacgct tccagatctg ctcgccgtcc gcggtcaacc
     1741 acagcttcgt gccgagcagc gccacggagc caccactcaa ctcgaatggc agaagcaccg
     1801 tgccggtgcc gaacgtggtc tcgccgtaga gctcggcgcg atcgttgtcg cgcaggcccg
     1861 aggagacgat ctccgaggcg gaggcggagc cctcgttgat caggacgacg aggtcgccct
     1921 cctgccattc gcccgaattg ccgatggtgg tcacctcgtg caccgagccg tcggcgttct
     1981 gctcctgata cagcacgctg ccaccgggca ggaactgcga gccaatgccg atcgcctcga
     2041 agacgaggcc gccagggttg ttgcggaggt cgaggatgac gccggtcatc ccctgcgcct
     2101 tgccctcgcg gagcgcctcg acgacgccct cggtcgcgcc ggccgagaac tgcgagaccc
     2161 gcagccaaag cacgccgttc ggcatcatca gcgaggaaac cggatcgatc tcgatacgcg
     2221 cgcgggtaat ggtgaccgtg tagggctcag cctcgtcctg gtgccgcagg gtcagggtca
     2281 catcggtgcc tgcctcgccg cggatcaggt cgaccagctc ctcggggtca acttccgtcg
     2341 attgcacgcc atcgaccgca aggatgacat cgcccgagcg gatacccgcc tcgaaggccg
     2401 gtgatccgtc gatcggggcg acgaccacgg gctgaggtcc ggtggtgtcc acctggatgc
     2461 cgatgccgat cagctcgccg cgcgaggagc gctcgaactc gtcggcctcg actggcgtga
     2521 ggaatgtgga gtggccctcg tcacccaggg cctcgaccat gccggtcgcg gccccgtaca
     2581 tgagctcctc gtcggagaat tcctcggaca ggacgtagtt ctcccggatg aggtcgtagg
     2641 tctcctcgat cacctggaac tccgggatct gcgtgagcat cgtcgaagcg ttcgcgttat
     2701 cggcgctgaa cctggcgccg agcatgccac cgaaggtgat ggcgagcgcg atgagaaggg
     2761 agagcgagag ccagcggctc acgggtggac ggcggttcgc ctggctggag ccatcgcgac
     2821 catggatgcg agaacgggtc atgcggtctc ctgtgggcag tttgggcatc aggcatcgcc
     2881 agatgcaggg gtgttgccgg agcatgtgaa cggggttgcg cctgtaaggg gttatacgtc
     2941 ctgcacacca tccagattag caaggaatgg gccgcaccta tggtgcctca ctacctatct
     3001 ggcgccccga ggcgc
//
LOCUS       DASYWL010000121         4430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_61978, whole genome shotgun sequence.
ACCESSION   DASYWL010000121 DASYWL010000000
VERSION     DASYWL010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61978"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1991)
                     /gene="ligA"
                     /locus_tag="VGR22_04360"
     CDS             complement(<1..1991)
                     /gene="ligA"
                     /locus_tag="VGR22_04360"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEV2127835.1"
                     /translation="MTDAVERRLDELRQTLDRWNYEYYILDAPSATDAEYDELMNELR
                     SLEAEHPDLVTPESPTQRVGAEAQSTFNQIEHVRPMLSLSNVYNEEELRAWAERAERF
                     AGTDNLRFVTEPKIDGVAVAITYENGAFAYAATRGNGLVGDDISPNVRTLRSVPLHLR
                     KSDRFPTPARVEIRGEVYMRRSDFEALNERMADAGNKLFMNPRNAAAGSLRQKDVQVT
                     AQRPLRMFAYQIGYVEGIPQPRSHWETLEMIREYGFAVSPDALLHNSIDEVWARGEYW
                     LERRAVLDFEIDGMVVKVNDLAHQEEIGYVAREPRWATAYKFPAIQQVTKLLDIVINV
                     GRTGTLNPSAVLEPVNIGGVTVSRATLHNEDEIARKDLRIGDTVLVQRAGDVIPQIVK
                     VMEDRRTDDEVPYEMPDHCPSCGQPVHREPGEAMRYCTNAACPAQLRERIHHFIGRSA
                     MDIDGVGEKLADRFVDLGWLHDAADLYTLDYSAVADLEGLGEKSAENIRNAIEASKRQ
                     PLWRVIHGLGIRHIGERTAQLLADRFHSLEALGQASVEEIADVNGIGQIVGRSVVDFF
                     SEEPNRELVRKLTEAGVQTSQEPPEQNAEQVLDGMTLVLTGRLETHTRSEAEAVLRGL
                     GANVAGSVSKKTTAVIAGEAAGSKAEKARELGIPVLTED"
     gene            2098..4272
                     /locus_tag="VGR22_04365"
     CDS             2098..4272
                     /locus_tag="VGR22_04365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEV2127836.1"
                     /translation="MASRRSLWCSMTLAGSEDQSDSTGHYEALSESAVSFIEWRGGSV
                     HEDALVLHVFGRSTSPALWRSLLQRALAEEPRLTYTADGHWMLAAGARTAPLVEESLL
                     PRFVALDVETTGLKATSHRIIEVALIRHENGIEVDRFDRLVNPGRTIPAYISKLTGIR
                     NDDVADAAVFGTLVDDIVTFIGEDPVIGHNVAFDIGFLNAELSRVHIGRVVNETIDTL
                     TLATRLIPGLRRPSLDRVARAVGITPRKIHRAGTDAAMAAEIALRLEAEARRQGIASF
                     DALKALSSANEPGRRNKVGRGRTLTDRSLLDGIPQAPGVYIMRDADGQVIYVGKAKNL
                     RDRVRTYFSQPMGYARKMDGLVEAIARIDTEVVGSELEALLLEAQLIRRYTPRYNTMM
                     RAFEHYPYIRVDVGNRWPRVTLVKDLKDDGARYFGPYRSKSAARKTVDVINDVLPLRT
                     CTRSFKDARSYGNPCIRLALGKCMGPCVDVSIRGDYLETVHQIIRFLDGDDDALYHVL
                     WGALEEAAERQDFDRAEKLRRNLATVNSIVNGQRGLRQAVECHHLVLVVPSVNEGERE
                     LWLVLEGRIWSRTSVGPPVRDDAVLSHAPQRDGGGEAEDRVLPEISDVSREQSIPTDE
                     VVERLAVSYRRAQASGVPPISHDSLDDIHILNRWIAAHSDHPAVLVLDRDRVDDGAYW
                     REMVERALGVPDEHLVLEVTGGAPEASEGAAATGQGRGSAPA"
BASE COUNT          737 a         1415 c         1482 g          796 t
ORIGIN      
        1 tcgtcctcgg tgaggaccgg gatgccgagt tctcgcgcct tctcggcctt gctgccggcc
       61 gcctcgccgg cgatgaccgc cgtcgtcttc ttcgacaccg acccggccac attggcaccc
      121 aggcctcgca gcaccgcctc cgcctcggag cgggtgtgtg tctccagccg tccggtcagc
      181 acaagcgtca tgccgtcaag cacctgctcg gcgttctgct cgggcggctc ctgtgacgtc
      241 tgtacccccg cctcggtcag cttgcggacc agctcgcggt tcggctcctc gctgaagaag
      301 tcgacgacac tcctgcccac gatctgaccg atgccgttga cgtcggcgat ctcctcgacc
      361 gatgcctgcc ccagtgcctc cagggagtgg aagcggtcgg ccagcagctg ggcggtacgc
      421 tcgccgatgt ggcggatgcc gagaccgtgg atcacccgcc agagcggctg ccgcttgctc
      481 gcctcgatcg cgttgcggat attctcggcg ctcttctcgc cgagtccttc gagatcggcg
      541 accgccgagt agtcgagcgt gtagaggtcg gctgcgtcat ggagccatcc aaggtcgacg
      601 aagcggtcgg ccagcttctc gccgacaccg tcgatgtcca tcgccgagcg cccaatgaag
      661 tggtggatgc gctcccggag ttgtgcgggg catgcggcgt tggtgcagta gcgcatcgcc
      721 tcacccggct cgcggtggac cggctgccca cacgatggac agtgatcggg catctcgtag
      781 ggcacctcgt cgtcagtgcg acggtcctcc atgactttca cgatctgcgg aatgacatcg
      841 ccggcccgct ggacgagcac ggtgtcaccg atacgaagat ccttccgggc gatctcgtcc
      901 tcgttgtgca gcgttgcgcg ggagacggtc acgccgccga tgttgaccgg ctccaggact
      961 gccgacgggt tgagcgtgcc ggttcgaccg acgttgatga cgatatcgag cagcttcgtg
     1021 acctgctgga tcgccgggaa cttgtaggcg gtcgcccagc gcggctcgcg tgcgacgtag
     1081 ccgatctctt cctggtgcgc gaggtcgttt accttgacga ccatgccgtc gatctcgaag
     1141 tccagcacgg cgcggcgctc cagccagtac tccccgcgtg cccagacctc gtcgatcgag
     1201 ttgtgcagca gcgcgtctgg cgaaaccgcg aacccgtact cgcggatcat ctccagcgtt
     1261 tcccagtggg aacgcggctg cgggatgccc tcgacgtagc cgatctggta ggcgaacatc
     1321 cgtagcggcc gctgggcagt gacctgcacg tccttctgcc ggagggagcc ggcggcggca
     1381 ttgcgcgggt tcatgaagag cttgttgccc gcatctgcca tgcgttcgtt gagcgcttcg
     1441 aagtcggagc ggcgcatgta gacctcgccg cgaatctcga cgcgggccgg tgtcgggaag
     1501 cggtcggatt tgcggagatg cagtggcacc gagcggagcg tgcggacgtt cgggctgatg
     1561 tcgtcgccga ccaggccgtt gccgcgcgtg gcggcgtagg cgaaggcacc gttttcgtag
     1621 gtgatggcga ccgccacgcc gtcgatcttc ggctcggtga cgaaacggag attgtcggtg
     1681 cccgcaaagc gctcggcacg ctctgcccag gcgcgcagct cctcctcgtt atagacgttg
     1741 ctgagggaga gcattggccg gacgtgctcg atctggttga aggtcgattg tgcctcggca
     1801 ccgacacgtt gggtgggcga ctccggcgtg accaaatccg ggtgctcggc ttcgagggag
     1861 cgaagctcgt tcatgagctc gtcgtattcg gcatccgtgg cggagggggc gtcgaggata
     1921 tagtactcgt agttccaccg gtccagagtc tgccgcagct cgtcgagccg gcgctccacc
     1981 gcgtcggtca tctgggggcc tgctcctttg tccgtgcgtc acggatgcgc tataacgaca
     2041 cagcgtcgta gtggcgaacg tacagcctgt ggattctagc acgcaggaat cctgtcgatg
     2101 gcatctcgcc gctcactttg gtgcagcatg acccttgccg gatcggaaga tcagtcggac
     2161 agcaccgggc actacgaggc actcagcgaa tctgccgtca gctttatcga gtggcgtggc
     2221 ggcagcgtcc acgaggatgc cctcgtactg cacgtcttcg ggaggagcac ctcgccggcg
     2281 ctctggcgat cgttgctgca gcgcgcgctc gccgaggagc cacgcctcac ctacaccgcc
     2341 gacggccact ggatgctcgc tgccggcgct cggacagcgc cgttggtcga agagagcctg
     2401 ctgccgcgct tcgtggcact ggacgtcgaa accaccggtc tcaaggccac cagtcatcgg
     2461 atcatcgagg tcgccctgat ccgccacgag aatggcatcg aggtcgatcg cttcgaccgg
     2521 ttggtcaacc ccggccgcac catccctgcg tacatctcga agctgaccgg catccgcaac
     2581 gatgatgtcg ccgacgcggc ggtatttggc acactggtcg atgacatcgt cacgttcatc
     2641 ggtgaggacc cggtcattgg ccacaacgtc gcattcgata tcggctttct caacgccgag
     2701 ctgagccgcg tgcatatcgg ccgcgtagtg aacgagacga tcgacacgct cacgctggcg
     2761 acccggctca tccccgggct gcggcggccg agtctcgacc gggtcgccag ggcggtcggc
     2821 atcaccccac gaaagattca ccgtgccggg acggatgccg cgatggctgc ggaaatcgcg
     2881 ctgcggctgg aggcggaggc acggcggcaa ggcattgcct cgttcgatgc gctcaaggca
     2941 cttagctcgg caaacgaacc cgggaggcgg aacaaggtcg gtcgcgggcg cacgctgacc
     3001 gaccgaagcc tgctggacgg catcccccag gcgcctggcg tctatatcat gcgggatgcc
     3061 gacgggcagg tgatctacgt tggcaaggcg aagaacctgc gcgatcgcgt ccgaacctac
     3121 ttctcccagc cgatgggcta cgcgcggaag atggacggac tggtggaggc gatcgcgcgg
     3181 atcgacaccg aggtggttgg ctccgagctg gaggcattgc tgcttgaggc gcagctgatc
     3241 cgacgataca cgcctcgcta caacaccatg atgcgcgcct tcgagcacta cccgtacatc
     3301 cgagtcgatg tcggcaatcg ctggccgcgc gtcacactgg tcaaggatct caaggacgat
     3361 ggcgcacgct acttcggccc gtaccgcagc aaatccgccg cacggaagac ggtggatgtc
     3421 atcaatgacg tgctgccgtt gcgaacctgc acgcgctcgt tcaaggacgc gcgctcctat
     3481 ggcaatccct gcatccggct ggcgctcggg aagtgcatgg gaccgtgcgt cgacgtctcc
     3541 atccgcggtg actatcttga gacagtccat cagatcatcc ggtttctcga cggtgacgac
     3601 gacgcgctct accacgtgct ctggggcgcc ttggaggagg cggccgagcg acaggacttc
     3661 gaccgtgccg agaagctgcg ccgcaatctc gccacggtca attccatcgt caacgggcag
     3721 cgcggactgc ggcaggcagt ggagtgccac cacctcgtgc tagtggtgcc ctcggtgaac
     3781 gagggcgagc gcgagctttg gcttgtgctg gaggggcgca tctggtcgcg gacgtcggtg
     3841 ggcccaccag tgcgcgatga cgcggtgctg tcgcacgccc cgcaacgcga tggaggcggc
     3901 gaggccgagg accgcgtgct gccggagatc agcgacgtga gccgtgagca atccattccg
     3961 acggacgagg ttgtcgagcg attggcggtg tcctaccggc gagcgcaggc gagtggcgta
     4021 ccaccgatct cgcacgattc actcgatgac atccacatcc tcaaccgctg gatcgccgca
     4081 catagtgacc atccggccgt gctggtgctg gaccgcgacc gcgttgacga tggtgcatac
     4141 tggcgggaga tggtcgagcg ggcactcggc gtgccggatg agcacctggt gctcgaggtg
     4201 accggtggtg ccccggaggc atcggaagga gcggcggcaa ccggccaggg gcgtgggagc
     4261 gcgccggcgt gatgttcgtc gggtcgatgc tgcgacattg acggccaccc cgcagtgtgc
     4321 aaagatgccc gcacacgact gacaatcggc gctgcctcga tgtcccgact gatagtgtcg
     4381 tggcagcaag ctggaaggca acgagctcat gaccatgcaa cgtcgaacac
//
LOCUS       DASYWL010000122         2136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_437389, whole genome shotgun sequence.
ACCESSION   DASYWL010000122 DASYWL010000000
VERSION     DASYWL010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437389"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(571..735)
                     /locus_tag="VGR22_04370"
                     /pseudo
     CDS             complement(571..735)
                     /locus_tag="VGR22_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012786061.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1041..2012
                     /locus_tag="VGR22_04375"
     CDS             1041..2012
                     /locus_tag="VGR22_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV2127837.1"
                     /translation="MRSLKLGIFLPIFESPDDSLFPGLLRWPELLALVRRIEAAGFDS
                     VWVPDHLLIRWDGERGPAAGAWEGWSLLAALAAATEHVELGTLVLCTAFRNPALLAKM
                     ADTVDEISNGRLILGLGAGDHEPDFRAFGYPFDYRVSRFAEALTIITRLLRDGAIDFD
                     GTYYSARACELRPRAARPGGPPIMVGGGFPPGPRMVRLGVEHADIWNHWLAFDRSYPD
                     ALPPLRQQVDAVCATIGRDPTMLRRTVTVAVVPPGHQPAHTAPHVESVTGSAEEIGGV
                     FRDFARVGGDHLQLLLNPCNEAAVDALLPVLNLLDQGASADRHASGE"
BASE COUNT          336 a          691 c          712 g          397 t
ORIGIN      
        1 gccaggttgg tcgagtcgaa gttcggccac cctgccgcct cctcggcagc cgcgtcgatc
       61 gctgcctcgc cgccccgcga gccgcccgtg ccagagactg actgggcgga gagcagggtg
      121 agcagcagca cggcgagcag ggcgatgcgg aacgagggcg agtgtgtggt catgacgagc
      181 ctcctgttga gtcaactggg gcgggtcggt tcccggccac ctgtcgccat catcggggct
      241 cggataggtg cacgacatcg tgcatttact cgatatccgc cggcatgcgt agattgcacc
      301 cgttacgtat cgctcgtggc cacagcgccc cgtcggcgtg cccacacact cacggccacc
      361 accaccgggc cgttaatcag cagcgacgtt ggccgggtct cggccctcca gcagggcgat
      421 ggagcgcgct tcttgcgaga tcggcacgaa tctggtggtg ctgtgagcgt cccgaccctc
      481 ggcgacaact gccttgcagt ccgcatcgat tcgtccgcgg cggcttagag cgaccggcaa
      541 taggcgctcc ttgagtctcc gggtggtggg tcagccgggc cagcggtcgc gggatcgcgc
      601 gcgttggatc aagcatcggg tggtggtgaa cgcggcggcc aagaacaggt aaacttcgac
      661 gaccgcagta tcccgctccg tgcagcggcg cagcttgccg aagccattca tccacgattg
      721 ggtgcgctcg accagtagcg tgcggctcgc ctcatcgggt tcgtgatgct gatcgcgact
      781 tagagcttgc gcgaataggc gcagggggga gtgtggtcac aaattcccag ggtagctgcg
      841 tcacgcttga cggatgccgg cccgacgctc aggttgctgt ggctgcgttg ccctcaccgc
      901 ggcaccgatc cctcgccgta cggagcattc cgccgtgatg cccaagaacg gatatccctt
      961 gaccatatct ccaattccca catcgtcata cgataggcca ttgggtccgg gtcgacgatc
     1021 gtcgcagaca ggaacgcacc atgcgctcgc tcaagctcgg catcttcctt cccatttttg
     1081 aaagccccga cgacagcctc ttcccgggac tccttcgctg gccggagctg ctcgccctgg
     1141 tcaggcgcat cgaggcggct ggcttcgatt ccgtgtgggt tcccgatcac ctcctgatcc
     1201 gctgggatgg ggagcgaggg ccggccgcgg gtgcatggga gggctggtcg ctgctggcgg
     1261 cgctggccgc cgcaacagag cacgtcgaac ttggcaccct cgtgctttgc actgcgttcc
     1321 gcaacccggc cctgctcgcc aagatggccg atacggtcga cgagatcagc aacggtcggc
     1381 tgatcttggg attgggcgcg ggcgaccacg agcctgattt ccgggcattc ggctacccct
     1441 tcgactaccg cgtgtcccgc tttgcggagg ccctcaccat catcaccagg ctcctgcgtg
     1501 acggcgcgat tgactttgat ggcacctact actcggcgcg cgcctgtgag ctgcggccgc
     1561 gcgccgcgcg tccaggaggt ccaccgatca tggtgggcgg tgggttcccg ccaggtccgc
     1621 ggatggtgcg cctgggggtt gagcacgctg acatctggaa ccattggctg gccttcgacc
     1681 gcagctatcc cgacgccctc ccaccgctcc gccagcaggt cgatgcggta tgtgcgacga
     1741 tcggccgcga cccgaccatg ttgcggcgca cggtcaccgt cgcggtcgtt ccgccggggc
     1801 accagccggc ccatacggca ccgcacgtcg agtccgtgac ggggagcgcc gaggagatcg
     1861 gtggggtatt tcgtgacttc gcccgcgtgg ggggcgacca tttgcagctg ctgctgaatc
     1921 cctgcaacga agcggcggtc gatgcgttgc tacccgtgct gaatctcctg gatcaaggtg
     1981 cgagcgctga tcgacatgcg tcgggggagt gagcactgat cacctcgatg ccgttgaaac
     2041 ggccctattg gcgcaagctc taagacgtgg aagatctagt tgccgccggg ggtggtggtg
     2101 tcgatctgct cggtcagcga gcgaaagccg atgccc
//
LOCUS       DASYWL010000123         3432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_437406, whole genome shotgun sequence.
ACCESSION   DASYWL010000123 DASYWL010000000
VERSION     DASYWL010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437406"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..223)
                     /locus_tag="VGR22_04380"
     CDS             complement(23..223)
                     /locus_tag="VGR22_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127838.1"
                     /translation="MIEVGVNTMVQRIVAPGMQGRVFANLYGFIGVAAGASYAAGGPL
                     LDATGPRVLLIIAGLGGLIATA"
     gene            complement(321..509)
                     /locus_tag="VGR22_04385"
     CDS             complement(321..509)
                     /locus_tag="VGR22_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127839.1"
                     /translation="MITETNIMGVPEIRNYECRVIEGAVIVTYEVRGPGERDWQPVRL
                     ISLTSWRSYGTRIAGQRN"
     gene            complement(506..1039)
                     /locus_tag="VGR22_04390"
     CDS             complement(506..1039)
                     /locus_tag="VGR22_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127840.1"
                     /translation="MYERNLCPKSETYRFTHPGEPCWHCKSRTEQRAILDAKQQANVE
                     IFNITEATPAASNVENSDIPPAPAVSPVQNLDTPPVTFGGHTITAPGFTPEQFERAIA
                     RALSDELVVHEAAGYHLVTHRSVNGGYHTMRTSCDSKAGEYGQPCKHRAILCFHLDVR
                     EPAIRRQWTDAQKAVAA"
     gene            1211..1429
                     /locus_tag="VGR22_04395"
     CDS             1211..1429
                     /locus_tag="VGR22_04395"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2127841.1"
                     /translation="MRSDYMTIGEAADALGVTRTTVWRRIKSGELEVFRSQTDRRERL
                     VRRADIEAMLRPAETDQGKGNPAHNLTA"
     gene            1604..2368
                     /locus_tag="VGR22_04400"
     CDS             1604..2368
                     /locus_tag="VGR22_04400"
                     /inference="COORDINATES: protein motif:HMM:NF026010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV2127842.1"
                     /translation="MTDGKKSRRGHNKGGKYKGDRYYIEALSLGYTTNRQGKRVRARK
                     YFYGKTRREAEEKKNRWLTEREREERLRAKGIDVDHHRMPFAEFVEEWFSTSVTGKVK
                     PSTESNYRNAVKSHLLPAFGDTPLNEIRPQAGERMLRQKEGQLKPSTLKALLTVLRTI
                     LNTAVRWRYIERNPASDVRHDFGDIRGNRRYLSHEQEAALFAQDMAPWERSLYRLAID
                     TGLRQGELLALQWSDINTRAGHPDGDPQPHPRQSGV"
     gene            2328..2822
                     /locus_tag="VGR22_04405"
     CDS             2328..2822
                     /locus_tag="VGR22_04405"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV2127843.1"
                     /translation="MVTRNLTRAKAVYDEDGNKITGALQFTTPKTKAGVRIVPFPPAS
                     ATALRNQQVYVEQLRTKATTWTEHDLIIPSRVGTPLSPTTVGDRFRRRRDAAGIPDAV
                     FHSFRHTATSRLMLDNIPHPIAMALLGHKNIDTTMLYTHASEEELIETMRAYFETKNE
                     HHDE"
     gene            3001..>3432
                     /locus_tag="VGR22_04410"
     CDS             3001..>3432
                     /locus_tag="VGR22_04410"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HEV2127844.1"
                     /translation="MEFLIVFLLIVLNGLLAMSELAVVSAKPSRLERRAEEGSAGARV
                     ALELAEQPDRFLSTVQIGITLIGVGTGALGGATLAGPVGELLARIPGIGETTATSIAG
                     IVVVIGLTYLSLVIGELVPKRVALQRAEAMAVLMSRPLKVMA"
BASE COUNT          640 a         1078 c         1091 g          623 t
ORIGIN      
        1 cgtgcgggag ctgcaaggcc attcatgcgg tcgcgatgag gccgcccagc ccggcgatga
       61 tgagcagtac gcgcggtccg gtggcatcga ggagcggccc tcccgcggca tacgacgcgc
      121 cggcggccac gccgatgaac ccgtacaggt tggcgaacac ccgtccctgc atgccgggcg
      181 ccacgatccg ctggaccatc gtgttgacgc cgacctcgat catgctgatc cccgtccggc
      241 gcgctcaacg atggctgccc gctgcgtcag tttatcccgc aggtgcatcg tgttggccgg
      301 ccacatggtg gtagccaagt tcaattcctc tggccagcga ttctggtccc gtacgacctc
      361 cacgaggtga gggagatgag ccggaccggc tgccagtccc gctcaccggg accacgaacc
      421 tcataggtga cgatcacggc gccctcgatc acccggcact cgtagttgcg aatctccggc
      481 acgcccatga tgttcgtctc ggtgatcatg ccgccaccgc cttctgggcg tcggtccact
      541 gtcggcggat cgccggctct cgcacgtcga ggtggaagca gaggatcgcg cggtgcttgc
      601 agggctggcc gtactcgccg gccttggagt cgcagctggt gcgcatcgtg tggtagccgc
      661 cattgacgct gcgatgggtg acgaggtggt aaccggcagc ctcatgaact accagctcgt
      721 cactgagtgc ccgggcgatg gctcgctcga actgctccgg cgtgaagccg ggtgcggtga
      781 tcgtgtgccc accgaaggtg accggtggcg tgtccaaatt ttgcacgggg gagacggccg
      841 gagctggagg gatgtcggaa ttttcgacat tcgaggcggc cggagtcgcc tccgtaatgt
      901 tgaaaatttc aacattcgcc tgctgcttcg cgtcgaggat cgctcgctgc tcggttcggc
      961 tcttgcagtg ccagcagggt tcaccgggat gggtgaaccg gtaggtctcg ctcttcggac
     1021 aaaggttccg ctcgtacatg ttgccctcct tcggcttgcc gggcgaggta ccagctcgct
     1081 tcggctcaga tacaggtgag aggtgttcta ctctctgtaa cgtgttgtat cccaccacag
     1141 cttgtggcgc aacattttta accgcactgt gatatatttt ggagagaatt cgtgcgcgac
     1201 ggagggcgtg atgcgaagcg actacatgac gatcggcgag gctgccgatg cgctcggagt
     1261 cacccgtacg acggtctggc gccggatcaa gagtggcgag ctcgaggtct ttcggtcaca
     1321 gactgatcgt cgcgagcggc tggtgaggcg ggccgatatc gaggcaatgc ttcggccggc
     1381 cgaaactgac cagggaaaag gaaaccccgc tcacaacctg acggcctgag cggggcatga
     1441 acacctctca cctgttggta ccaagtgagg gcatgctcat tgtaccaatt ctttcgtttt
     1501 attcgtttgt ttcgtgccta tcacgagagc ccggaaaagc ccgtaactct gggtaaacgg
     1561 ggaattacta cgtttctttc gttttcttcg tagggcaccg accatgacgg acgggaagaa
     1621 gtcccggcgc gggcacaaca agggcggcaa gtacaagggc gaccgctact acatcgaagc
     1681 cctcagcctt ggctacacca ccaacaggca gggcaagcgc gtccgtgccc ggaagtactt
     1741 ctatggcaag acccgccgtg aggccgagga gaagaagaat cgatggctca cggagcgcga
     1801 gcgcgaggag cgcctgcgag ccaagggcat cgacgtcgac catcaccgca tgcccttcgc
     1861 tgagtttgtc gaggagtggt tctccacctc ggtgaccggc aaggtgaagc cgtcgacgga
     1921 gtccaactac cgcaatgccg tcaagagcca tctcctgcca gcgttcggcg acacgccgct
     1981 caatgagatt cggccccaag ccggcgagcg gatgctccgg cagaaagagg gacagctcaa
     2041 gccgtcaacg ctcaaagcgc tcctgaccgt gctgcgaacg atcctcaaca ccgctgtacg
     2101 ctggcgctac atcgagcgca acccggcatc cgacgtgcgg catgacttcg gcgacatccg
     2161 cggcaaccgc cggtacctgt cacacgagca ggaagccgcg ctcttcgctc aggacatggc
     2221 gccatgggaa cggagcctgt accgactggc gatcgacacc ggcctgcggc aaggcgagct
     2281 cctagcgttg cagtggagcg acatcaatac tcgagcgggg catcctgatg gtgacccgca
     2341 acctcacccg cgccaaagcg gtgtatgacg aggacggaaa caagatcaca ggggcgctgc
     2401 agttcacgac gccgaagacg aaggccggcg tgcggatcgt gcccttcccg ccggcgtcgg
     2461 ctaccgcgct acgaaaccag caggtctacg tcgagcagct gcgcacgaag gcgacaacct
     2521 ggaccgagca cgatctcatc atcccctcac gtgtcggcac tccactctcg ccgacgacgg
     2581 tcggtgatcg gttccgccgc cggcgtgatg cggcagggat ccccgacgcc gtcttccact
     2641 cgttccggca taccgctacc agccggctca tgctcgacaa catcccccac ccgatcgcca
     2701 tggcactact gggccacaag aacatcgaca ccacgatgct ctacactcac gccagcgaag
     2761 aggagctcat cgagacgatg cgggcctact tcgagaccaa aaacgagcac cacgatgaat
     2821 agtggttgta cgtttggcat tacacctcga catattgata gtgtcgtata ttgtgttcgt
     2881 gtacgagatg ccggtgtttc ggggcgtttc gagggaacac agccgacggc aggcgggatc
     2941 tattgcactc tatcgcagat catccgagct gcggtgccga caagggagtc tgtggtcgga
     3001 gtggaattcc tgatcgtctt cctgctcatc gtcctgaacg gcctgctggc gatgtccgag
     3061 ctggcggtgg tctccgcgaa gccgtcgcgg ctggagcggc gtgccgagga gggcagcgcc
     3121 ggcgcgcggg tcgccctgga actcgccgag cagccggacc ggttcctgtc gaccgtccag
     3181 atcggcatca cgctgatcgg cgtcggcacc ggcgccctcg gtggcgcgac cctcgccggg
     3241 ccggtcgggg agctgctggc ccgcatcccc ggcatcggcg agacgaccgc gacgagcatc
     3301 gccgggatcg tcgtcgtgat tggcctcacc tacctctcgc tggtgatcgg tgagctggtg
     3361 cccaagcgcg tggccctgca gcgggcggag gccatggccg tcctgatgtc ccgcccgctc
     3421 aaggtcatgg cc
//
LOCUS       DASYWL010000124         1706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_174541, whole genome shotgun sequence.
ACCESSION   DASYWL010000124 DASYWL010000000
VERSION     DASYWL010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174541"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VGR22_04415"
     CDS             <1..234
                     /locus_tag="VGR22_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127845.1"
                     /translation="AMSSDITDETQDAAEDYFRLIDGIPEQAWYWLALGSIGVSAALK
                     LMDKDHWELFVGQWLPTFLLFGLYHHVMRPSQR"
     gene            500..742
                     /locus_tag="VGR22_04420"
     CDS             500..742
                     /locus_tag="VGR22_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127846.1"
                     /translation="MIEEIAVRPLLGQPHRERERWLRHQVREAGCRLHRSRRDEDRYM
                     IFDLEQDGIAAGDAFELTLDDVAAWIGDREHLQPDL"
     gene            1016..1603
                     /locus_tag="VGR22_04425"
     CDS             1016..1603
                     /locus_tag="VGR22_04425"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEV2127847.1"
                     /translation="MSPDDRTHKNYDDVNLSGADLSESDLSSASLRRSQLKGANLQNA
                     TLAGSDLHGAHLDHANVTGADLRGADLTGASLHGVDLDRAATIVGARFAGATGVTGDR
                     TADDDTQALEDHVAQAAEDIVLITRDIAALASAASGPDDEDRSSIPERIAELQRRRSL
                     LEERMLSLIDDAAQEAIAAHGGVAANNLSAKAARE"
BASE COUNT          322 a          561 c          558 g          265 t
ORIGIN      
        1 gccatgagca gcgatatcac ggacgagaca caggacgccg ccgaggacta cttccgcctc
       61 attgacggca tccccgagca ggcctggtac tggctggccc tcggctcgat cggggtctcg
      121 gcggcgctga agctgatgga caaggaccac tgggagctct tcgttggaca gtggctgccg
      181 accttcctgc tgttcggcct ctaccaccac gtgatgcgcc cgtcccagcg ctagcatccc
      241 ggcacccgtc tggccccgtc gacatttgga cgcagctgac attgccggcc ccgccaacct
      301 agggccggcg caactgtggt tctcctacct gcgtgcacca ggcgattttc ccgtgctgga
      361 accacgccgc atcctgagca tgctccttcg aggaccacgt cctacaccgt cccgcctcag
      421 tcgtcagcgc gacggcgtaa ccggcagtca cgacccagtg gcgctgcacg ggtcgctgga
      481 acatcgaaag gacatcacca tgatcgagga aatcgccgtt aggccactgc tcgggcaacc
      541 tcaccgggag cgagagcggt ggcttcgcca tcaggtgcgt gaggcggggt gtcgcctcca
      601 ccggtcgcga cgggacgagg atcggtacat gatctttgac ctggagcagg atggcatcgc
      661 cgccggggat gcgttcgagt tgacgttgga tgacgtcgcg gcgtggattg gcgatcgcga
      721 gcatctacaa ccggatttgt aaggagcgcc tcgaccgccc ggcacgccac tgggtggcgc
      781 cggcacgtca ccatggcgaa caagagcgcc gagccatgct tgtcggaaga ccgagcagcg
      841 tgccgagaca ggctcgacgc tgacgcggcc gccacaagcc gacgcctcgg tggacaaccg
      901 tggcactcaa gctgcatacc gagaatgcca ccatcagcgc gctgccacgt cgacagtgct
      961 ctcggtcgca tccgtgctga cccgtacaaa gcggagagtc gccaaggagc gttgtatgtc
     1021 accagatgac cgcacgcaca agaactacga cgatgtcaat ctctctgggg cagacctgtc
     1081 ggagtcggac ctgtccagcg cctcactgcg ccgatcgcaa ctgaaaggcg ccaatctgca
     1141 gaatgccaca ctcgcggggt cggacctgca cggcgcccac ctcgaccatg ctaatgtgac
     1201 cggcgcggac ttgcgcggtg cggatttgac tggggccagt ttgcatgggg tcgacctcga
     1261 ccgggcggcg accatcgtgg gtgctcggtt cgctggcgcc acgggggtca ccggcgaccg
     1321 cacagccgat gacgataccc aggccctaga ggaccatgtg gcgcaggctg ccgaggacat
     1381 cgtgctcatt acgcgggaca tcgctgcact ggcgagcgcc gccagcggac ccgatgacga
     1441 ggaccgcagc agcatcccgg agcgcattgc cgaactgcag cgccggcgga gtttgttaga
     1501 ggagcggatg ctgtcgctga tcgacgatgc cgcgcaggaa gcgatcgccg cccacggcgg
     1561 cgtggcggcg aacaacctga gcgcgaaggc cgcccgagaa tagggggaac actcgcccat
     1621 gcactcgcgt tcgattactg gcattgcata ccggaagcag ggacgcgaac cagcgtcacg
     1681 gggccggccg acggcagggc cggccc
//
LOCUS       DASYWL010000125         4084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_381113, whole genome shotgun sequence.
ACCESSION   DASYWL010000125 DASYWL010000000
VERSION     DASYWL010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381113"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(323..766)
                     /locus_tag="VGR22_04430"
     CDS             complement(323..766)
                     /locus_tag="VGR22_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127848.1"
                     /translation="MASESPHLLDLIHLERRDVLTGGSKPCTRERWKVRLWSEQVDGR
                     LATRTRESGRFRASRLRALRGKRDEREQQRSHHNPSPHEHKCRLSPYLCQREWMIGAR
                     SAPCCTASDRPAEDRHLGALAIVSHSTHGSVPWCTLSEWSTSTNG"
     gene            complement(2027..2947)
                     /locus_tag="VGR22_04435"
     CDS             complement(2027..2947)
                     /locus_tag="VGR22_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127849.1"
                     /translation="MKRISAEAYQALREALAVIVWNKRPFESYLRAALRDSPELLAGL
                     PFAEPKRAVADLLVDRLLRDERRYQEVTLNLMLEIASMTSFPNIELIKDEEDRAVRME
                     NAQRAVGHLRGLVQGYAELVTEREKAIAEREARRSQDEAQRTFSDEVDQLRRRFLTLQ
                     EEPNPQRRGRDLEMLLRDLFLLFDMEPRLSYDLDREQIDGSLSFDTDDYIVEARWRAE
                     PSSRGDADIFAAKVRRKGKNALGLFVSVAGMSRDAIEQYSESTSFILLDGSDLYLVLD
                     QRIRLDDLLKVKKRHANETGACFLPASTVL"
     gene            complement(3274..3399)
                     /locus_tag="VGR22_04440"
     CDS             complement(3274..3399)
                     /locus_tag="VGR22_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127850.1"
                     /translation="MDSPSPEELIAKCPVHGHRPYNDPDKRPPFRQRALRRPRKD"
BASE COUNT          698 a         1324 c         1298 g          764 t
ORIGIN      
        1 cctacgtgcc gctcgcgcgg gctctcgacc gacagcggcc gacggcccgg cgttgagggt
       61 aggagcaccg aacggtcctt cgcgccggcc tcgcacatcg gcggtgtccc ctatggacat
      121 tgctcaagcg aagacgtagt cgaggaggcg ctccttgccc gccgccatgc ccctatgccg
      181 atccagtacc agaacagcag gcccagcggc atcgagagga ggccccaagg gtccgcttgg
      241 cggaaccgcc acaccgcgtc gaagacaccg ccggccgcta gcactgaggc aaccacggcc
      301 agcgcgtacc acgcccctcg tttcagccgt tcgtggatgt cgaccactcg ctcagtgtgc
      361 accagggaac ggaaccgtgc gtgctatgac tcacgatcgc gagcgcgccg aggtgccgat
      421 cctcagcggg tcgatccgag gccgtgcagc agggcgccga gcgcgcgccg atcatccatt
      481 cgcgctggca gaggtaggga gacaaccggc atttgtgctc gtgtggcgat gggttgtgat
      541 gggatcgttg ctgctcgcgc tcgtcgcgtt tgccccgcag agcgcggagg cggctggcgc
      601 ggaatcggcc cgactcacgg gtccgggtag cgagtcgccc gtcgacctgc tcgctccaga
      661 gccggacctt ccagcgctca cgggtgcatg gtttgctgcc gccggtgagg acgtcacgcc
      721 gctccaggtg gatcaggtca aggagatggg gactttcgct ggccatacgc ggggactttt
      781 acatggccat gggcactcgc atcggtgacc tcagccccat tgactacgaa gccagctgca
      841 cgcccgccgc tgctgcggca tgatcaccac atgacagctt cttttggtta ataattaacg
      901 agtgacttcc tttatcaagg accaccaaac agcttccggc atacttggct ttgtaatttc
      961 atcgataata gctacgatct atctttttgt agtaccagat gaggcagctg attctaaggg
     1021 tttagtcgcc atagcaattc tatacgcgca tcctgcttgt tgggtgcttt tggccattgc
     1081 cagcttattg ttcgcatgcc gagccgcaaa gaagctcatt aagtttatag gtttggcggc
     1141 tctggcatgc tatgtgacct ttatagctac cgtcttggtt gcataactat caagtagata
     1201 tggtcggctg ctggtgtcca cggtgccggg attcggtgaa gaactagctc gatttgacag
     1261 cattgggtcc ggttctgacc tccggcgccg accacacctc cttcaaacgt ccgaggtcgc
     1321 gtcccccggc cgggccccac atcgagcatt gacgcgcccc ggcggtcacc tcaaccgttg
     1381 ggcaatgccc gatcgcccgc gccgtcgacc cctcccagat cgccggcatg acgacagcct
     1441 ggattatcgt ggaacctaat ggagcagcgg gggatggctc ttggcgtggc gatcggcgcc
     1501 ggcgtcgcgg tcggcgtcgt tacgggaagt ctcgtctttt ggctcccatt gggcgccggg
     1561 gttggcgttg ccattggcgc tctactcggc cgccaacgct gacagggcag tcaacgttcc
     1621 tgcatcgagg cgctctctat cgccgggtca acttgccacc tatcagggcg ccgacgggca
     1681 cagccggccg caccgatccg gcagagatcg tcggatcgga cctggacggc ttcaacacga
     1741 gccgcaccga ggtgccgctc gcgcggacct cgaggggtgg ccgccgacgg cgcggcatcg
     1801 aaggtgagga gcaccgaacg ctcttcgcgc tggcctcgga ctccgacgac ccggcaatcg
     1861 gttcgccccc gggcccggta cgcactcggc ggtcccatcg gagctttcta acggttgcgg
     1921 cgagccctgt ctcggcgatc tgtcaccaca atgaacagac cgagcacgac gaccgccaga
     1981 cccacggcaa tcgcagcagc ctctaattcc tgggccggtc aggcctctaa aggaccgtcg
     2041 aggcaggaag gaagcaagct ccggtctcgt tggcgtgccg cttcttgacc ttcagcaggt
     2101 cgtccagcct gatccgctga tcgaggacga ggtacaggtc tgagccgtcg aggagtatga
     2161 acgaggtcga ctcgctgtac tgctcgatcg catccctcga catcccagcc acgctgacga
     2221 acaagccgag ggcgttcttt cccttccgcc ttaccttcgc agcgaagatg tcggcatcgc
     2281 cgcggctcga gggttccgca cgccaacgag cctcgacgat gtagtcgtca gtgtcaaagc
     2341 tcaaggagcc gtcgatttgc tctcgatcaa ggtcgtagga gagcctcggc tccatgtcga
     2401 agagcaggaa gaggtccctc aggagcatct cgagatctct ccctcggcgc tgcgggttcg
     2461 gttcttcctg caaggtgagg aaccgccggc gcaactgatc aacttcgtca gagaacgttc
     2521 gttgagcttc gtcctgtgac cgccgcgcct cgcgctcggc gatggccttt tctcgctcgg
     2581 tcacgagctc cgcgtatccc tgaaccagtc cccgcagatg acccaccgcg cgctgagcat
     2641 tctccatgcg aacggctctg tcctcctcat ccttgatgag ctcgatgttg gggaacgacg
     2701 tcatggacgc gatctccaac atgaggttca gcgtcacctc ctggtagcgg cgctcgtctc
     2761 tgaggaggcg gtcgactagg aggtcggcga ctgcgcgttt cggctcggcg aacggcagcc
     2821 ccgcgaggag ctctggtgag tctcgaaggg ctgcgcgcag gtacgattcg aacgggcgct
     2881 tgttccagac gatcacagcg agtgcctcgc gaagcgcctg atacgcctcc gctgagatcc
     2941 gcttcacgtc ccaacggtat tcggaactcg cttcgcccgg tgtgaccgcg caccgcgccc
     3001 cggatcctgc tccgcggttt ccagcgcccg gatgcccctg gtacgagcag ccgccgaaat
     3061 gcctgatggc gtggggcgga ccgcgggacc gggggccagc gtcggaggca ccgacgagta
     3121 ccgaaccttc cgttgcgctg gcccgcgtca accgtgagcc gtcctcttac ggaatctgag
     3181 agtgtgcgcc gttgagcaag ggcaatgaag tgctgctcgc tacgcatacg cgccaccact
     3241 gtgcctagca gttcaggggt tagatgatgc ggactagtcc tttcgtggtc gtcgcagggc
     3301 gcgctgacgg aacggcggtc gcttgtccgg atcgttgtac ggacggtgtc cgtgcacggg
     3361 acacttggcg atcagctcct cgggggacgg actgtccacg aagggtcctg cagggccggg
     3421 aacgatcgct ccgaccgggt acggcccaac gaggtcacgg ccgcagaccg gacaacgaat
     3481 ccaggcctcg gggacccgtt ggggcggtgg ttcgaagacc cggcggagaa cgctgagcaa
     3541 ggcgctgacg accgccacgc tccgagcgta cgtcacctgc cctgtccctg gctgtcgctc
     3601 ggacgtgccg ctggtttcac cagccgcttg ccgcctcctg aggcgatgct ggcccgccgc
     3661 cctgtctacc tggatgtcag aggcagccgc cagacgagat gtttgtgtct tcggggctct
     3721 tgaggaccca cggctcgcgc ttggtgaggc gcagcaacac cgctaccgca gtagccgccg
     3781 caccgaggtg ccgctcggcc gcgccggcac cggcggccga cgccggagcc aaggcagcgg
     3841 cctacccgcg ccgattatcc gctcgcgctg gtctcgacgg ctcgcgagcg ggcaatagcg
     3901 cgggcgtcac agggaggcat ggcatcctcg tgatcgtggg agcccagttt gccgacttgg
     3961 cgcagagagt gtcccagcgg tgtcggctca ccggcgagtt cacgctgcgc tcagggcaga
     4021 ccgccagcac ctacttcgat aagtacctgt tcgagggcga cccagggatt ctccgggatg
     4081 tcgc
//
LOCUS       DASYWL010000126         7930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_268572, whole genome shotgun sequence.
ACCESSION   DASYWL010000126 DASYWL010000000
VERSION     DASYWL010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7930
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268572"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            354..515
                     /locus_tag="VGR22_04445"
     CDS             354..515
                     /locus_tag="VGR22_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127851.1"
                     /translation="MDTGRERVEEAGEQDPSTTGQVVRTAGIATGMFFTVVILAIVAA
                     VLILWFVLA"
     gene            complement(620..1906)
                     /locus_tag="VGR22_04450"
     CDS             complement(620..1906)
                     /locus_tag="VGR22_04450"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEV2127852.1"
                     /translation="MGAGDVSVRLQPDLARKLGTGDAVVIGLGSMIGAGVFAAFGPAA
                     QSAGSAMLVGLAIAAFIAYCNAVASAQLAAAYPVSGGTYIYGRECLGHWWGFIAGWGF
                     VIGKTASCAAMALTFAVYAVPDSWWLQRLTAVAAIVALAVLNYRGITKTAGLARVLVA
                     ITLIALGIVVGGIFFAGNPRRDHLDGWFFGSTNGLYGVLQAAGLLFFAFAGYARLATL
                     GEEVRDPETTIPRAIPLALFLTVGIYALVGTSALLAASPERLAESTAPLVTAIRGAGV
                     ADIGPVVRIGAMVASLGALLALIAGIGRTSLAMARHGDLPGYLAAVHPRYRVPHHAEL
                     TLTLVVSGLVLLTDLRGAIGFSSFGVLVYYAVANASAWTQTGNDRRWPRWLNTLGLVG
                     CLVLVATLPVGAVLTGIAMFAIGIVGRVFVHRRQRR"
     gene            complement(2062..3000)
                     /locus_tag="VGR22_04455"
     CDS             complement(2062..3000)
                     /locus_tag="VGR22_04455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEV2127853.1"
                     /translation="MIGVAPENRSLTRFAWLTIAAAVATIGLKAGAYLLTGSVGLLSD
                     ALESVVNLVAGIATLVALWVAAREPDEEHAYGHTKAEYFSSGLEGLLILVAAGSIIWA
                     AITRLIDPVPIERVGAGLIVSLLATVVNGGVAWLLFRAGDRYRSIALRSNARHLMTDV
                     WTSVGVVVAVGLVAVTGWNRLDPVIALIVAANISWSGAVLVNRSVHGLLDTALDVEDS
                     ERLEVVLARYRERYGVRMHALRTRQAGTRRFMSVHVLVPGEWTVRRGHDLLDQLEHDL
                     RQELPDLTVMTHLEPLEDPASWDDMHLDRGQRQESW"
     gene            complement(3151..3468)
                     /locus_tag="VGR22_04460"
     CDS             complement(3151..3468)
                     /locus_tag="VGR22_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEV2127854.1"
                     /translation="MAWLVLVTSGMLEAVWATALGRSDGLTRVGPAIIFVLAVVLSML
                     GLGIAMREIPVGTAYAIWVGIGAALTAGYAMASGGEPVSAVRVLLLAGIVGCVVGLKL
                     VTP"
     gene            complement(3584..3657)
                     /locus_tag="VGR22_04465"
     tRNA            complement(3584..3657)
                     /locus_tag="VGR22_04465"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3621..3623),aa:Glu,seq:ctc)
     gene            complement(3709..4095)
                     /locus_tag="VGR22_04470"
     CDS             complement(3709..4095)
                     /locus_tag="VGR22_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2127855.1"
                     /translation="MQFTEVMGGRIDRQAPRILVVDDEPGIVDLLCMLLEEEGFEAAG
                     VTSGYGAAEMIRSERPDLMLTDVMMPGMTGYDLAELAAGVHPQMRVVLMSAAVDPPSK
                     SRFPFVSKPFDLGNVVHVIDETLRAP"
     gene            complement(4220..4915)
                     /gene="pdxH"
                     /locus_tag="VGR22_04475"
     CDS             complement(4220..4915)
                     /gene="pdxH"
                     /locus_tag="VGR22_04475"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEV2127856.1"
                     /translation="MRRSNATPVQTEVPRGLMKSRTSSMRREYERAVFDEDTASTDPF
                     EQFRLWFDEAAASPTIEPNAMVVATVSADGQPSQRTVLLKSWDGTGFVFYTNYESRKG
                     RDLEQNRQVSLLFWWADHERQIRVEGRAERTSPGESDAYWQQRPRGSQIGALASPQSQ
                     VVRNREWLAHRFAQIEAEHAGGEPVCRPHHWGGIRVVPTMFEFWQGRPNRLHDRLCYR
                     LQRGIWVVERLAP"
     gene            5167..7593
                     /locus_tag="VGR22_04480"
     CDS             5167..7593
                     /locus_tag="VGR22_04480"
                     /inference="COORDINATES: protein motif:HMM:NF024688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HEV2127857.1"
                     /translation="MVTMRQAPPRSEDQHISGLSPDEMSALQEIERRILWLSTLIVHH
                     ANNVRENPDKPTKVGGHQASSASVVSILTALYFHFLRTGDRVSIKPHASPAYHAVQYL
                     LGNLDRRYLTMLREFGGLQSYPSRTKDPDPVDFSTGSVGLGAVAPVFAALAADYAEIK
                     FGEVTSNRFISLIGDAELDEGNVWETVAEDAVADLSNVIWIVDLNRQSLDRVIPGIKA
                     ARLKALFAGAGWNVFEAKYGTWLESVMSSEHGSALRARIDDMSNEEYQALIRIEDGAE
                     LRRRLADVADRKRHNAILASVKDIPDDRLQVLIANLGGHDLPKLIQVLNKADAVTNAP
                     SVIFAYTVKGWGLPIAGDPLNHSQLLTNEQMEELRQQFGIPESEEWEPVPSDTPAGKQ
                     VVQAAERLRQERMVNTIPTDRVPDAISARHPNRTSTQEAFGRTLLRLGEIPDVGERVV
                     TMSPDVATSTHLAGWINKAGVFSREPEQDFEEGRKRMLRWQPSPEGQHIELGISEMNL
                     FMALSQFGVAADLTGQPLVPIGTVYDPFVCRGLDALIYGLYINARFIFAGTPAGVSLS
                     PEGGAHQSTVTPSLGIELPNLNFYEPAFGQELDWIMLEAVRQVLDREQGRSTYLRLST
                     KPIDQTLMEEPLARLGEETLRQHVLAGGYRLIDRTVDTPNLPDGDVVHIATGGIMVSE
                     AFVAARRLQEEGVAVNVISVTSPQRLYNSLTAARAGFLKDAWGKPDFGHLSTLFPANE
                     RRAPLVTVQDGASHSLAFLGSVWGAPVVPLGVDEFGQSGSRGDVYRKVGIDTDQIFNA
                     GLLALDLLEG"
     gene            complement(7724..>7930)
                     /locus_tag="VGR22_04485"
     CDS             complement(7724..>7930)
                     /locus_tag="VGR22_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127858.1"
                     /translation="RLQGAAGARDLQVWLSGSRGDDEFILLLRRMITGAYKGRNRVLE
                     MPARAQGKPPGLKAASIDTSRRLF"
BASE COUNT         1493 a         2714 c         2471 g         1252 t
ORIGIN      
        1 cgaggcctac gggcagcagg aggccgagct ggagcgtaag gccggccagg atgacccaca
       61 acgcgatgag gccgagagca ttaccgatct ggagcgcgag gcggaggagc gactcgaccg
      121 agtccgcaag gaagtcggcg acgacgagaa ctgagctcct tcgtcccgcc gggtgaggag
      181 tcaccatgga ccatcgatag cccaccgacg agacgctttc cgatcagtct atctcgtcgc
      241 gaccgcattc atcggaacat ttcgaagacc gcggcacgac cgacacgatc ggtcgcgatg
      301 ccggcaacga tcctcgggcc gctcgtgcgc gacgtggcga gcgcaccacg ggagtggaca
      361 ccgggcggga gcgcgttgag gaggcagggg agcaggatcc gtccacgacg ggccaagtcg
      421 tcaggaccgc cggtatcgcc accggcatgt tcttcaccgt cgtgatcctg gcgatcgtcg
      481 ccgcggtcct gatcctctgg tttgtcctgg cgtaacgacc gcaggtcaca gtgaaaggga
      541 cgcccgctgc cgtgcctgcg ctcatgggtc ttggccgtgg tacccaacgg cgatccttag
      601 atggtcacaa agcccgtcgt caccggcgct gtcgcctgtg aacgaagacc ctgccgacaa
      661 tcccgattgc gaacatcgcg ataccggtca acaccgcccc gaccggaagg gtcgccacca
      721 gtaccaggca cccgacaagc ccaagcgtgt tcagccaccg aggccagcga cggtcgttac
      781 ccgtctgcgt ccaagccgag gcattggcga ccgcatagta gaccagcacg ccgaaggacg
      841 agaagccgat cgcgcctcgc agatctgtca gcaggaccag tccgctgacc accagcgtga
      901 gcgtgagctc ggcatggtgt gggacacggt atcgcgggtg cacagcggca aggtagcccg
      961 gcaggtcgcc gtggcgagcc attgccaggc tcgtgcggcc gatgcccgcg atgagcgcca
     1021 acagcgctcc gaggctggcg accatcgccc cgatgcgcac caccggaccg atgtctgcaa
     1081 caccggctcc gcggatcgcc gtcaccagcg gcgcagttga ctccgcgagc cgctccgggc
     1141 tcgcggcgag cagcgcgctg gtaccgacga gcgcgtagat accgacggtc aagaagagcg
     1201 ccagcgggat ggcgcgaggg atcgtcgtct ccgggtcgcg cacctcctcg cccagggtag
     1261 cgagccgagc gtagccggcg aacgcaaaga agagcagccc ggcggcttgc agtactccat
     1321 agagaccatt cgtgctgccg aagaaccagc cgtcgagatg gtcgcgcctc ggattaccgg
     1381 cgaagaaaat gccaccgacg acgatgccca gcgcgatcag cgtgatggcg acgagaaccc
     1441 gcgcgagccc ggcagtcttt gtgattcccc ggtagttgag cacggcgagc gcaacgattg
     1501 ctgccacagc cgtcagccgc tggagccacc aggagtctgg cacggcatat acggcgaagg
     1561 tcagcgccat ggcggcacag gaggccgtct tgccgatcac gaatccccag cccgcgatga
     1621 agccccacca gtgcccaaga cactctcgac cgtagatgta ggttccgccc gatacaggat
     1681 aggctgcggc gagctgggcg gaagcgacgg cgttgcaata ggcgatgaag gcggcgatcg
     1741 cgaggccgac aagcatcgcg ctgccggccg actgggcagc gggaccgaag gccgcgaaga
     1801 caccggcacc gatcatcgac ccgagcccga tgacgaccgc gtcaccagtt cccaacttcc
     1861 ttgccagatc cggctgcaac cggacactaa cgtcgccggc tcccatactc actggtcgct
     1921 cactccccta ccctcagcga tactccgtga cactgtagcg cggcgacggg acgctcgata
     1981 tggaggatcg aaccgcatcg tcaatgtctg acgaactccc caccgtgatc caggcccggt
     2041 gcgactcggc agcggctgag ctcaccagga ttcctggcgc tggccacgat caaggtgcat
     2101 gtcatcccac gacgccggat cctcgagcgg ctcgaggtgc gtcatcacgg tgaggtcggg
     2161 aagttcctgg cgtaggtcgt gttccaactg atccagcagg tcgtgaccgc gcctgacggt
     2221 ccactcgccc ggcacgagca cgtgcaccga catgaatcgc cgggtgcccg cctgacgggt
     2281 gcgcagcgcg tgcatgcgga cgccatagcg ctcccgatag cgggccagga ccacttcgag
     2341 gcgctcggaa tcctccacgt ccaaggcagt gtcgagcaag ccgtgcacgg accggttgac
     2401 gagcacggct cccgaccagc taatgttcgc cgcaacgatc agggcaatga cggggtcgag
     2461 gcgattccac ccagtcacgg cgaccagccc gacagcaacg acaacgccga cggacgtcca
     2521 gacgtccgtc atgaggtggc gggcattgga gcgcagtgca atggagcgat accggtcgcc
     2581 ggcacggaaa agaagccagg cgacgccacc gttgaccacc gtcgccagca gcgacacgat
     2641 cagtcccgcc cccactcgct cgatgggtac cggatcgatg agccgagtga tggcggccca
     2701 gatgatggag ccggctgcga cgaggatcag caatccctcc aggccgctgc tgaaatactc
     2761 ggccttcgta tgcccgtagg cgtgctcctc gtccggctcc cgcgctgcga cccacaacgc
     2821 cacgagggtt gcgataccgg ccacgaggtt caccaccgat tcgagcgcgt cggagagcag
     2881 gccaacggag ccggtgagca ggtaggctcc cgccttcagt ccgatggtgg cgaccgcagc
     2941 tgcgatcgtc agccaggcaa atcgagtgag cgatcggttc tccggagcga cgccaatcat
     3001 caatccaggc ccctgcttgt gacgaagctg cggttggcgt caccgagcgt agtcgaccct
     3061 cctggcttca tagggccaaa tgcagccggc ctccggtgat cttgggagat ccgcacgcta
     3121 tatggcgaag ggagaaggag ccgcccgcag ctagggcgtg acgagcttga ggccgactac
     3181 acagccgacg attccggcaa gcagcagaac gcgaacggcc gacaccggct ccccgccgga
     3241 cgccatcgca taccctgctg tgagcgccgc cccgattccg acccagatgg catacgccgt
     3301 gccgaccgga atctcccgca tggcgatgcc caacccgagc atgctgagaa ccaccgcgag
     3361 cacaaagatg atggccggcc cgactcgggt caacccatca gatcggccga gcgcggtcgc
     3421 ccacaccgct tcgagcattc cggaggtcac cagcaccagc cacgccatcg gttaacctcc
     3481 gcgtgtgcca gacgacgtgg ccgggagtcg tgccgacctt ccgctcctcg ccgggccgga
     3541 tacgatgaaa cccccgcgcg tgcgggggtt tctgtctcgt tcgtagcgca taggggattc
     3601 gaacccctga tctccgcctt gagagggcgg tgtcctaggc cgctagacga atgcgcctca
     3661 ttcactgtaa aaaaactata ccaaaccctt ttcggacgcg caaacccctc atggcgctcg
     3721 cagcgtctcg tcgataacgt gcactacgtt gccgaggtca aacggcttcg acacgaacgg
     3781 gaaccgcgat ttcgacggcg ggtcaaccgc cgcgctcatc agcacgaccc gcatctgtgg
     3841 gtgcacacca gccgcaagct ccgcgagatc atagccagtc atgccgggca tcatcacatc
     3901 cgtcagcatg aggtccggtc gctcactgcg gatcatctcc gccgcgccat acccgctggt
     3961 cacgccggca gcctcgaacc cctcttcctc gaggagcatg cagagcaggt cgacaatgcc
     4021 tggctcgtcg tccacaacga gaatgcgggg tgcctgtcga tcgatcctgc cccccatcac
     4081 ttccgtgaac tgcattgtcc tgtcctgcac gctgaccatc tcgctaatcc cgtgtttccc
     4141 atccccggtc gtcgtcgatc gcaccgggta ccaatgatga accgcacgca agtgggctgc
     4201 cacggaactc aggcaggctt cacggagcca ggcgttccac cacccagatt ccgcgttgca
     4261 ggcgatagca cagtcggtca tgtagccggt ttggacggcc ttgccagaac tcgaacatcg
     4321 tcggtacgac cctgatgcca ccccagtgat gcggacggca gaccggctcc cctccggcgt
     4381 gctcggcctc gatctgggcg aaccgatgag ccagccactc ccgattgcga accacctggc
     4441 tttggggaga ggccagtgcg ccgatctggc tgccccgcgg ccgctgctgc cagtaggcgt
     4501 ccgactcccc cggggaagta cgctcggcgc ggccctcgac gcgaatttgc cgctcgtgat
     4561 ccgcccacca gaagaggagc gaaacctgcc ggttctgttc aaggtcccgg cccttgcggc
     4621 tctcgtagtt ggtgtagaac acgaacccgg tgccgtccca cgacttcagc aggacggttc
     4681 gctgggacgg ctggccgtcc gcactgacag tcgccacgac catcgcgttt ggctcgatcg
     4741 tcggcgacgc agccgcctcg tcgaaccaga ggcgaaactg ctcgaacggg tcagtgctgg
     4801 ccgtgtcttc atcaaacacg gcccgctcgt actcccgtcg catgctggac gtgcggcttt
     4861 tcatcaaacc cctcggaacc tctgtctgca ccggcgtggc gttactccga cgcatgatcc
     4921 cgcgtcatac tcgaaatgac aacccgagcg ttgcgttgtg ccgtccgctc gaccctccac
     4981 caggcagctc agcgccttta gcccatccga cgacccaggc gaactgctga cgcgccggct
     5041 cggattgggt acgatagagc gaagagcggg aacggcacgg catcgaccgg cgtggcgcgt
     5101 ggcgtcggcg ccagagaacc ctcgacggcg agggttctgc ttcccacacc ccaggagtcg
     5161 tccctcatgg tgaccatgcg acaggcgccc ccacgttccg aggaccagca catctccggg
     5221 ttgtcaccgg acgagatgtc cgcactgcag gagattgagc gccggatcct ctggctctcg
     5281 accctgatcg tgcatcacgc caacaatgtc cgcgagaatc ccgacaaacc gaccaaggtg
     5341 ggcggccacc aagcgagctc ggcgtcggtc gtctcgattc tcaccgcgct ctacttccac
     5401 ttcctgcgca ccggcgaccg cgtctccatc aagccgcacg cctcacccgc ctaccatgcc
     5461 gtccagtacc tgctcggcaa cctcgaccgc aggtacctga cgatgctccg cgaattcggc
     5521 gggctccagt cctacccctc gcgcacgaag gatccggatc ccgtcgactt ctcgacgggg
     5581 tcggtcgggc tcggcgcggt ggcgcccgtc tttgccgcgc tggctgcgga ctacgccgaa
     5641 atcaagttcg gcgaggtcac ctcgaatcgc ttcatctccc tcatcggcga cgcagagctc
     5701 gacgagggca atgtctggga aacggttgcc gaggacgctg ttgccgacct gagcaatgtg
     5761 atctggatcg tcgatctcaa tcggcagagc ctcgaccgcg tcatccccgg gatcaaggcg
     5821 gcgcggctca aggccctgtt tgcgggtgcc ggctggaatg tcttcgaggc caagtacggc
     5881 acctggctcg agtcggtgat gagcagcgag cacggttccg ccctgcgcgc ccgcatcgac
     5941 gacatgtcga acgaggagta ccaggcgctc attcggatcg aggacggtgc cgagttgcgg
     6001 cggcgcctcg ccgacgtcgc cgaccgcaag cgtcacaacg ctatcctcgc ctcggtcaag
     6061 gacattccgg acgaccgcct gcaggtgctg attgccaacc tcggaggcca tgatctcccc
     6121 aagctgatcc aggtactcaa taaagcggac gcggtgacca atgccccgtc cgtcatcttt
     6181 gcctacacgg tgaaggggtg gggcctaccg attgcgggtg acccgctcaa ccactcccaa
     6241 ctgctcacga acgagcagat ggaagagctg cgacagcagt tcggaattcc ggaaagcgag
     6301 gagtgggagc ccgtcccctc ggacacgcct gctggcaagc aggttgtcca ggcggccgag
     6361 cgtctccggc aggagcgcat ggtcaacacc atcccgaccg atcgcgtccc ggacgctatc
     6421 agcgcgcggc acccgaacag gacctcgacc caagaggcat tcggccgcac actgcttcgc
     6481 ctcggggaga ttccggatgt gggcgagcgc gtcgtcacga tgtcgccgga cgtcgccacc
     6541 agcacgcacc tggcggggtg gatcaacaag gcaggtgtct tctcacgcga gccagagcag
     6601 gacttcgaag agggcagaaa acgcatgctg cgatggcagc cgtcacccga gggccagcac
     6661 atcgagctcg gcatctcgga gatgaacctc ttcatggcgc tcagccagtt cggcgtcgcc
     6721 gccgacctga ccgggcagcc gcttgtgccc atcggcacag tctacgaccc gttcgtctgc
     6781 cgtggcctgg acgcactcat ctacgggctc tacatcaatg cccgcttcat cttcgccggc
     6841 acgcctgcgg gagtctctct cagcccggag ggcggagccc accagagcac cgtcacgcca
     6901 tcactcggca tcgagctgcc gaacctcaac ttctacgagc ccgcctttgg tcaggagctc
     6961 gactggatca tgctcgaggc ggtgcgccag gtgctcgacc gcgagcaggg ccggtccacc
     7021 tacctgcggc tttcgacgaa gccgattgac cagacgctca tggaggagcc cctggcgcga
     7081 cttggcgagg agacgctgcg ccagcacgtg ctcgccggcg gatatcgcct gatcgaccgc
     7141 accgtcgaca cgcccaacct gccggacggc gatgtggtgc acatcgccac cggtgggatc
     7201 atggtgtccg aggcgttcgt cgcagcgcgg cggctgcagg aggaaggcgt ggcggtcaac
     7261 gtgatcagtg tgacgagccc gcaacgactc tacaattccc tcaccgcggc tcgtgccggt
     7321 ttcctgaagg acgcttgggg caagccggat ttcggccacc tctcgacgct cttcccagcc
     7381 aatgagcggc gggcaccgct ggtcaccgtg caggatggcg cgtcgcactc actggcgttc
     7441 ctcggctcgg tttggggagc cccggtggtg ccgctcggcg tcgacgagtt cgggcagtcc
     7501 ggctcgcgcg gcgatgtcta ccgcaaggtc ggcatcgata ccgaccagat cttcaacgcc
     7561 ggcttgctgg cactcgacct gctcgaaggc tgaagcgcct cgccgcagtc acgggcggcg
     7621 aggtgggcaa cgcatggccc atccctgcag gaagacaacc gtgaagagga agaggcccgg
     7681 accgtcaccc ggcccccttc cgggatcagc cgttgtccct gtttcagaac agccgtcggg
     7741 aggtatcgat cgatgccgcc ttgagtcccg gcggcttgcc ttgggcacgc gccggcatct
     7801 ccagaacgcg gttgcggccc ttgtaggcgc cggtgatcat ccgccggagc agcaggatga
     7861 actcgtcatc accgcggctt cccgagagcc agacctggag atcccgggct cccgccgcac
     7921 cctgcaggcg
//
LOCUS       DASYWL010000127        15708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_606496, whole genome shotgun sequence.
ACCESSION   DASYWL010000127 DASYWL010000000
VERSION     DASYWL010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 15708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15708
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606496"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1357)
                     /locus_tag="VGR22_04490"
     CDS             complement(89..1357)
                     /locus_tag="VGR22_04490"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV2127859.1"
                     /translation="MAETDRAAHRRLFSFDAAFAEGNPYPHYAWFRRHDPVHLKEPNW
                     PMGKRPALLFRHEDVHQWLRDPRMLREAEALEEVRDNPEYISWRHAPQDSFGYMAHRF
                     MLFRDPPAHTRLRGLANRAFTPRVVADRRAEIEELANSLVAAFREEGGEGDLIQALAY
                     PLPVLVIARVLGVPAEDMHRFRDWAAVLGAAIDLPTEGLEAFVARADEATHEMSEYLR
                     GIVAKRKVDPQDDLLTRLIVARDEEGRVTDDELIATAILLMIAGHETTVNLIANGALA
                     LMRHRDQWERLVAEPALVANATEELLRYDSPVQLTGRIATEDVEIDGVMIERGAQVMF
                     MLGSGNRDEALWEDADDLRIDREVGRYLSFGMGIHFCLGAPLAKLEGEVTFATLAREA
                     PNLELVDPNPKWRPGAVLHGLQRLDVRMEV"
     gene            1495..2712
                     /locus_tag="VGR22_04495"
     CDS             1495..2712
                     /locus_tag="VGR22_04495"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEV2127860.1"
                     /translation="MIQTSAPAIAAPVPPATEASAGPLTLRVEEAVVAEGNVIVANTN
                     TQNDAPPDGFAYVLARITVTNTGQQTRTVTASDFTATGTDGVLRRCPSIALPDPPLNV
                     TLAPGESVYGWTGGLVNDLSNVVLLFDPAISIGERFSATFALTDGAAIPTFDTASAET
                     DAGTSLDAPAALGETARTGMWEVTVIDAIGSDTYFEISDYRVRALGAPTDDPDSWRSL
                     GLEVTVRNITGAPQFFSWTALELVDTSGEPWDHLLATTQPYPSASVEMLPGASVTGWY
                     GIWLQPWATTSLLRLRDSALTDDFRYISLDGTAGSASSASEEPAGTEEVASEPPNLAP
                     GDLAKVGADPLNLRDEASTSGGIVAELDPGTEVVITSDAVEVDGYRWYQVAVTETGEA
                     GFVAEDFLTPAGE"
     gene            2985..3353
                     /locus_tag="VGR22_04500"
     CDS             2985..3353
                     /locus_tag="VGR22_04500"
                     /inference="COORDINATES: protein motif:HMM:NF024788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HEV2127861.1"
                     /translation="MTVQTAVMVALVAGLGTAQYIFTVQALRDLRRRPRVRGDNKTGW
                     TLLILCLPIVGALVYGWMGPTSLIRRPNPSESTSRPYPIGRPAPRRNITPISEARRRE
                     RPVSASSERGTIKRFRRTGS"
     gene            3392..4330
                     /locus_tag="VGR22_04505"
     CDS             3392..4330
                     /locus_tag="VGR22_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEV2127862.1"
                     /translation="MTQLAAVLIDDAFARHDTGMHPEHPRRHAAIHSAIAGMLPGRPE
                     IASDPASDEILLRVHTPDHLRELDAIAATCGAWVDPDTMVGPDSVDTARTAVGAAVAG
                     INAVLDGVTSRAFALGRPPGHHATASRAMGFCLLNSIAVAAAHARLRGIEQIAIIDWD
                     VHHGNGTQAIFEDDPAVWYASIHQSPLYPGTGAASETGRGAGEGTVCNAPMRAGSGDA
                     AWLEAFDTRVAPFVERARPDLILLSAGYDAHESDPIGGCMVTDDGFAALTERTVALAE
                     RHCDGRLVAVLEGGYDPPALGRCVARTIVTLDIGTA"
     gene            4398..5759
                     /locus_tag="VGR22_04510"
     CDS             4398..5759
                     /locus_tag="VGR22_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127863.1"
                     /translation="MLRRRKRQQQTQQDDAPETGSPPAPERLDEVWEEPVNEPAPDGA
                     GHDDDAEETQEPAPDQLQEAPRPQSRAVPRPETESRYRREDERRREEARTATLQSQVD
                     ELRALLRELSSRQQRAEEQGKHQQALVAQNALAIEQVEKQAEQNAQARALDENRTRQK
                     IDDLETRIEDGIRPIRSLQAHVTEILEESRKKVDNTAQNEQRFDELSTMIEHLSALGD
                     RTTTVTHGLRDTIDGVRGEIDGLRRDILRTEDAVKIVDQEARRRTTDVQEIAESYNGR
                     LDELRSDLAHLYDMIENTRRGLVHVDPTLDELRQAEESLRQEFTRHQALTEERHDMLI
                     DMHDDARQENDARFEQLRQNMEDRIDRLNERLEEANEMHRELSYRIGSINSEIEELRQ
                     IDTGLHRDIWYLHEQRVRIRMEQIQEELDAATAQRRDAESELQSGAGARSRRRRSNTE
                     GDV"
     gene            6405..9752
                     /locus_tag="VGR22_04515"
     CDS             6405..9752
                     /locus_tag="VGR22_04515"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEV2127864.1"
                     /translation="MSALLHKTRTGFFFAMIGMLIMSMMAPTVALANEDAQTSSNQAV
                     SNETVVSTESSESTTTSTTDENAVTEQSAPANPWQEPIDYGVANDNVKCRKAASTSAR
                     EITVIYEGQTIEITGVEIWAEGISWTPVNCAGVGGFISTGYISVETETVEEPEVQVVE
                     ETGVEESVVEETVTDQGGEQQVEQTGVEQVAQANASQTEEEETDEATSDEDQVEDAIE
                     EPAPVEQPVLESIATGYATSAVNCRVAPSIEAETIAGFAAGNAVPVTGDVFSTEGLNW
                     TPVNCAGVGGFVASDFITFEAPAPNNPSQESTENLAGASSVETTAPETADEPVEGNTV
                     PDGSADQALQTTSEPTGDAAQAGTENIEEPSLAEAIVEDVQSRVPDAEQIIDETQQQI
                     DESLGEAPEVEETAPDVGDESNATEGAEETATDVEDGGEPTIPDADELADEIIEDAQE
                     QVDESLGNPPAIAEATEEATAEVTEEVAEEVANTDTANPGMTGQEALDTQTTDEPDEP
                     VTTEETISEATEDTTDAGTEGATEEPQEEEGETHASATSGVAVEETESRTPIEDNAII
                     GTADINGTGDQGLPCRVSPEMDAPVITTLPENMSVFVLSEPDANGWMSIVCADRVGYA
                     PATYLYSGGATLTFDPAVTGTAVVNGTGGGGLNCRSGPSTGYSVIGWFAEGATVTLTG
                     KSSGVWVEVICGGTQGWAYGTYLGGHGSSGGDTTSDPDTNNQAPSSGTVTVSGTGGAR
                     LNCRTGASTSFPATTSVAEGTVLNVRGAASGGWLPVVCNGSDLWVYTQFVTAGGTATP
                     QQPKEETPSNGGGTDAVTGQATVVNTDGASLRCRSGAGLTHSTITFLPAGSTVSLRSG
                     SQGDWTAVVCAGQNGYAYSSYLSTSGGGSTAAPTPEPAPSQPSGLANGDHARTTANLN
                     LRYDPYAGNNVAAVAPQGTVVQITGGHAGNNYYPVNWDGLKGYMSADYLVETSEALSE
                     RGGSAPTTDSQTAGGGGGTSTGNAIADFAMGYLGYPYVWATAGPSSFDCSGFTYWVIK
                     NVVGIDIGRGLWTQIAAGTPVSRSALQPGDLVFFQNTYKAGLSHVGIYIGGNQFVHSQ
                     NEATGVVISDLDSSYYGPRWYGARRIG"
     gene            9850..10779
                     /locus_tag="VGR22_04520"
     CDS             9850..10779
                     /locus_tag="VGR22_04520"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEV2127865.1"
                     /translation="MPSPNDLDHLSLLLQRTVPDIGPVVGLEHVAEGYGATTLRALGG
                     LCIRIPHSAHLALKQNRTATALHRLAPLLPVPTPVPLWTLPVGYPFETGAIGYQWIDG
                     EQPDPETSPPSLAEQVGRFLATLHGIDGETLDTFDGRVPGPADVAAERDQVMGVALPW
                     LWERQSAGVMDWIERWWASFRAEREAARYELRLVHGDFWHGNLLADASQERLTGVIDW
                     EQIALDDQAQDLATLLHGGTAFSHAVLATYRAAGGTVDAATIARRDWLWGYREFTGIA
                     LALEFGDEAEALDGMHKLQQGALRHLFITGSEP"
     gene            10776..11570
                     /locus_tag="VGR22_04525"
     CDS             10776..11570
                     /locus_tag="VGR22_04525"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2127866.1"
                     /translation="MNHSELRSHRSTIDAVTLAWYEHGGELGSPIVCLHGLGASSLDF
                     AWLATWPAFRHMRLLLIDAAGHGASDRPDDWPYSIEAHAELIVGLLREIAGTPVALVG
                     HSMVCSMAIALAHRHPGLVDRLVLAEPNLDPGTGTLSGHVARQREERFVTRGYAALVY
                     QTERTAARGDLVAAGFLQSLRLASPMVIHRSAVSLRAERLPTFHEQLETMTLPRTLLR
                     GKRTPPLAPPLSTTAIQQVVIPGAGHQMMIDNPDAFATSIRTAIDE"
     gene            complement(11853..12938)
                     /gene="recA"
                     /locus_tag="VGR22_04530"
     CDS             complement(11853..12938)
                     /gene="recA"
                     /locus_tag="VGR22_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEV2127867.1"
                     /translation="MAAPQADRDRAVDMAIAQIERNFGKGSIMKMKPGEVNPMQVDAI
                     PTGSIALDLALGIGGVPRGRIVEIYGPESSGKTTLALHIIAEAQKMGGLAAIVDAEHA
                     LDPVYAGRLGVDLDELLISQPDTGEQALEITETLVRSGALDVIVVDSVAALVPKAEIE
                     GDMGDSHVGLQARLMSQALRKLTGAISRSKTCVIFINQLRMKIGVMFGSPETTTGGNA
                     LKFYSSVRMDIRRIETLKQGTDAIGIRARVKVIKNKVAPPFRQAEFDIMYNEGISVSG
                     SVLDVATDLGIVRKSGAWFYLDEERLGQGRENAKLFLKSNPDVLEDIREQIKAKSPEL
                     SGRITVGAKDGEADEDGVLAAVDNAEE"
     gene            complement(13650..14573)
                     /locus_tag="VGR22_04535"
     CDS             complement(13650..14573)
                     /locus_tag="VGR22_04535"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEV2127868.1"
                     /translation="MTGDVFQELPNGEVRAEVHGIVRSDLIALDGGTFCMGTNDPTGF
                     PTDGEGPVRPVSVSSFRISRYAVTNRQFRAFVEASGYVTEAERFGWSFVFHLFMPKRG
                     VTVRGASGAAPWWTGIDGASWNAPEGPGATIDDRLDHPVVHVSWNDAMAFCAWSSTRL
                     PTEAEWEFAARGGLDQKRFPWGDELMPGGKHCCNIWQGRFPDRNTTADGYFDTAPVDA
                     YEPNGFGLFNVAGNVWEWCADWWSPGPHPTEHRVDPIGPERGSTHVMRGGSYLCHKSY
                     CNRYRVAARTSNTPDSSTGNMGFRIAADTEG"
     gene            complement(14657..>15708)
                     /locus_tag="VGR22_04540"
     CDS             complement(14657..>15708)
                     /locus_tag="VGR22_04540"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEV2127869.1"
                     /translation="PALLIDRAAFHANIETMASALREHGTGWRPHAKAHKSPAVAHLL
                     IESGAHGITCAKTSEAEVYVASGIRVILIANQVVGPIKTRRLAHLARQADIAVAVDSI
                     ENAKEHDVAAAEAGTSPRLIIEVNCGMNRAGTEPGEATVELAKAIAGMSHVRFGGVMS
                     WEGHTLKVSDPEVRAQAIREALAPVLETVEAIRAAGIEVPIVSAGGTGTFLVTAGIEG
                     ITEVQAGGGIFGDRFYRDLGAPVQPALSLLVTVTSRPARDRIIFDAGRKSVDPSNLVP
                     EVIGLEGVSQLAFSAEHGTIRLDGPNCDVQVGDRLRLNIGYSDQAVHLHEQHFVVEAG
                     CVEAVWPTLARGRLQ"
BASE COUNT         2862 a         5412 c         4919 g         2515 t
ORIGIN      
        1 tgttgatact ggtcgggatc atgatcgccg ctcgggccaa ctcgcggcag gaggacgcag
       61 ggccgtaacg cgtacaggat gacgcgcgtt acacctccat gcgaacatcg aggcgctgga
      121 gaccgtgcag aacggcgccg ggacgccact tcgggttcgg gtcaaccagc tcgagattcg
      181 gcgcctctcg ggcgagcgtg gcgaaagtga cctctccctc cagcttcgcc agcggcgccc
      241 cgaggcagaa gtggatgccc atcccgaacg agaggtagcg cccgacctca cgatcgatcc
      301 gcaggtcgtc ggcatcctcc cacagcgctt cgtctcggtt gcccgagcct agcatgaaca
      361 tcacctgcgc cccgcgctcg atcatcacgc cgtcgatctc gacatcctcg gtcgcgatcc
      421 ggcccgtcag ctgcaccggg ctgtcgtacc gcagcaactc ctcggttgca ttggcaacga
      481 gcgcgggctc tgcaaccagc cgctcccact ggtcacggtg ccgcatcagt gccagcgcgc
      541 cgttggcgat cagattcacc gtcgtctcat gccccgcgat catcagcagg atcgccgtgg
      601 cgatcaactc gtcgtcggtc acccgcccct cctcatcccg cgccacgatc agccgcgtga
      661 ggaggtcgtc ctgcgggtcg accttgcgct tcgccacgat cccgcgcagg tactcgctca
      721 tctcgtgggt cgcctcgtcg gcgcgcgcga cgaacgcctc cagaccctcg gttggcaagt
      781 cgatcgcggc gccaagcacc gccgcccagt cgcgaaaacg gtgcatgtcc tccgctggga
      841 caccgagtac ccgtgcgatg accagcactg gcagcgggta ggcaagcgcc tggatcaggt
      901 caccttcgcc gccctcctcc ctgaaggcgg caaccagact gttcgctagc tcctcgatct
      961 ccgcacgtcg gtcggcgacg acacggggcg tgaacgcccg gttggcgaga ccgcgcagcc
     1021 gagtgtgggc cggcgggtcg cgaaagagca tgaaccgatg cgccatgtag ccgaagctgt
     1081 cctgtggcgc gtgcctccac gagatgtact ccgggttgtc ccgcacctcc tccagagcct
     1141 cggcctcacg cagcatacgc gggtcgcgca gccactggtg cacgtcttcg tgacgaaata
     1201 gcagcgccgg ccgcttcccc atgggccagt tcggttcctt gagatgcact gggtcgtgcc
     1261 ggcggaacca ggcatagtgc gggtagggat tgccctcggc gaacgccgcg tcgaaggaaa
     1321 acagccgtcg gtgtgcggcc cggtcggtct ctgccatggc ctcagtatct cccgtccata
     1381 tcgccctaca gcgtcctggt tccagtgtac ccgtcctccg gcgaccggtt cgcggtatcc
     1441 ttgccgggac gacatgccac cgcagcgcgt tctctcagga gcacagcaca cccgatgatc
     1501 cagacttccg cgccagccat cgccgcgccc gttcccccag ccaccgaagc ctccgccggc
     1561 ccgctcacgc tgcgggtcga ggaggcggtg gtcgctgagg ggaacgtcat cgtcgccaac
     1621 accaacacac aaaacgatgc accgcccgac gggttcgcct acgtgctcgc gcggatcacc
     1681 gtcaccaaca ccggacagca gacgcgtacg gtcaccgcct ccgatttcac ggccacgggc
     1741 accgacggcg tgctccgccg ctgtccgagc atcgcgctgc ccgacccgcc gctgaacgtg
     1801 acgctggcgc cgggcgagtc ggtctacggg tggaccggcg ggctggtcaa cgacctttcc
     1861 aacgtcgtgc tgctcttcga tccggccatc tccatcggcg agcgcttttc ggcgaccttc
     1921 gcgctcacgg acggagcggc gatcccgacg ttcgacacgg ccagcgcgga gactgacgcc
     1981 ggcacctccc tcgatgctcc cgccgcgctc ggcgagacag cccgcaccgg catgtgggag
     2041 gtgacggtca tcgacgcgat cggctccgat acctacttcg agatctccga ctaccgtgtg
     2101 cgggcgctcg gcgcgcccac cgatgaccca gacagctggc gatcgctcgg actggaagtg
     2161 accgtccgca acatcaccgg ggcgccgcag ttcttctcgt ggaccgcgct ggagctggtg
     2221 gacaccagcg gagagccgtg ggaccacctg ctggcgacaa cccagcccta cccatcggca
     2281 tccgtcgaga tgctgccggg cgccagcgtg acaggctggt atggcatctg gctgcagccg
     2341 tgggcgacaa ccagcctgct gcgactgcgt gactcggcgc tgaccgacga tttccgctac
     2401 atcagcctcg acggcaccgc tggcagcgcg agctcggcat ccgaggaacc agccggaacg
     2461 gaggaggtgg ccagcgagcc gccgaacctc gcaccaggcg acctcgccaa ggtcggcgcc
     2521 gacccgctca acctgcgcga cgaggcatcg acctccggcg ggatcgtcgc cgagctcgat
     2581 cccggcaccg aggtggtgat caccagcgac gccgtcgaag ttgatggcta ccgctggtat
     2641 caggtcgcgg taacggaaac gggcgaggcg ggattcgtcg ccgaggactt cctcacgccc
     2701 gccggcgagt agccgcgctc gacacgtcgc tcattgacgc gaacggtcgg cgcgccatga
     2761 caaccatcga cacctcgaca cacggttccc accggtgtgc cgtcggacgt ctcaggacgt
     2821 gctgcgacgt ggcgcgacat gatgtttcgt acgacaacac caccgctccc cggcagagat
     2881 ggcggatttt ggggtatatg tcgcacaatt ccctccaccc cgctacaata ggcgcagaaa
     2941 cgcgcgcaac gctgcgcgcg tcgctgctat ggaggatgcc cggcatgacc gtgcagacgg
     3001 cggtgatggt tgccctggtg gccggtttgg gcaccgcgca atacatcttc acggtccagg
     3061 cgctgcgaga tcttcgtcgg cgtccgcgtg tgcgcggcga taacaagacc ggctggacgc
     3121 tgctcattct ctgcctgccc atcgtcggcg cgctcgtcta tggctggatg ggccccacat
     3181 cgctcatccg tcgcccgaac ccgagcgagt cgacaagccg gccctacccg attggtcgcc
     3241 ccgctccacg gcgcaacatc acgcccatca gcgaagcccg gcgccgcgag cgcccggtca
     3301 gcgcgagcag cgagcgcggg acaatcaagc gcttccggcg taccggttcc tgacgccttc
     3361 catcccctga acccgaacgg gagatcttcc catgacgcag ctcgctgcgg ttctcattga
     3421 tgacgccttc gcgcgccacg atactggcat gcaccccgag catccgcgcc gccatgcggc
     3481 catccactcc gcaatcgccg ggatgctgcc gggccggccg gagatcgcct ccgacccggc
     3541 cagcgacgag atcctcctgc gggttcacac gcctgaccac ctccgcgagc tggatgcgat
     3601 cgccgccacg tgcggcgcat gggtcgaccc ggacaccatg gtcggcccgg actcggtcga
     3661 taccgcccgc acggccgtcg gggcggcggt cgccgggatc aatgccgtgc tcgacggtgt
     3721 cacctcacgt gccttcgcgc tcgggcgccc accggggcat cacgcaaccg ccagccgcgc
     3781 gatgggcttc tgcctgctga actcgatcgc cgtcgccgcc gcgcacgccc gtcttcgcgg
     3841 catcgaacag atcgcgatca tcgattggga tgtccatcat ggcaacggca cccaggcgat
     3901 cttcgaggac gatcccgctg tctggtatgc ctcgatccac cagtcgccgc tctaccccgg
     3961 cacaggtgca gcatctgaaa ccggccgggg ggctggcgag ggcaccgtct gtaacgctcc
     4021 gatgcgagca ggctcaggcg acgcagcatg gctcgaagcg ttcgacacgc gcgtggcgcc
     4081 gttcgtcgag cgtgcccgtc cagacctcat cctcctctcg gctggctacg acgcccacga
     4141 gagcgatccg atcggcggct gcatggtgac ggatgatgga tttgccgcac tgaccgagcg
     4201 caccgttgcc ctagccgagc gtcactgtga tggccgtctg gtggccgtcc tcgagggcgg
     4261 ctacgatccg cccgccctcg ggcgatgtgt tgcccgcacg atcgtcacac tcgacatcgg
     4321 gacagcttga cacccgtcgc cagccggtgg gcagcggtgt acccttggga aaacgtttca
     4381 ccctgatagg atcatccatg cttcgacgcc gcaagagaca gcaacagacg cagcaggacg
     4441 acgctcccga gacgggtagc ccccccgctc ctgagcgtct cgacgaggtc tgggaagagc
     4501 cggtgaacga gcccgcgccg gacggtgccg ggcatgacga cgatgcggag gagacgcagg
     4561 agccagctcc cgaccagctg caggaagcgc ctcggccgca gtcgcgcgcg gtgccccgcc
     4621 cggaaaccga gtctcgctac cgccgggagg atgagcgccg gcgcgaggag gcccgcacag
     4681 cgacgctgca gagccaggtg gacgagctca gggcgctgct ccgcgagttg agcagccgcc
     4741 agcagcgcgc cgaggaacag ggcaagcacc agcaagcgtt ggtcgcccag aatgcgctgg
     4801 ccatcgagca ggtcgagaag caggccgagc aaaacgccca agcccgcgcg ctggacgaaa
     4861 accgcacccg ccagaaaatt gacgatctcg aaacccgcat cgaagacggt atccgtccga
     4921 tccgctcgct ccaggcacac gtcaccgaga tcctcgagga atcacgcaag aaggtcgaca
     4981 acaccgccca gaacgagcag cgcttcgacg agctctcgac gatgatcgag cacctctccg
     5041 ctctcggtga ccgcaccacc accgtcaccc acgggctgcg tgacaccatc gacggcgtgc
     5101 gcggcgagat cgacgggttg cggcgcgaca tcctccggac cgaggacgcc gtcaagatcg
     5161 tcgaccagga agcgcggcgt cgcaccaccg acgtgcagga aatcgccgag agctacaatg
     5221 gccgcctcga cgagctccgt tccgatctgg cgcacctgta cgacatgatc gagaacaccc
     5281 gccgcggcct cgtccacgtc gaccccacac tggacgagct gaggcaggcc gaggagagcc
     5341 tgcgacagga gttcacgcgg caccaggcgc tcacggaaga gcggcacgac atgctgatcg
     5401 acatgcacga cgacgcccgg caggagaatg acgcacgctt cgagcagctc cggcagaaca
     5461 tggaggatcg catcgaccgg ctcaacgagc gcctcgagga ggccaacgag atgcatcgcg
     5521 agctgtcata ccgcatcgga tcgatcaata gcgagattga ggagttgcgg cagatagaca
     5581 ccggcctgca ccgcgacatc tggtacctcc acgagcagcg cgtgcgcatc cggatggaac
     5641 agatccagga agagctcgac gcggccaccg cacagcggcg cgatgccgaa tcggagctgc
     5701 agtccggcgc tggcgctcgt tcccgccgac gtcgcagcaa caccgagggt gacgtctaaa
     5761 tcgtcaccct cacacaccgg gtggagtgtt ggcgcctcta tcgtgccgaa caggcacatg
     5821 gacgcagcac gtgcgttcgt actaccattg cagacttcat caggtgcttg acagccgcat
     5881 acgcatttgt tagcttgcaa gaaacccgaa gggtttgatg gcgtcgagag gtggaacact
     5941 ctggctggtg acctcttctc gtggcatgac cggaaacgag cgacaactca cgcaggagca
     6001 ccgctacacg cattatcgga agctcggtca gtgatcgtct ctgagactcg acttccccgc
     6061 atgtgtgtgg ctcgtgctat acccgctata gtcgcccgca acggtgcttt gccaccatcc
     6121 gcccagggac ggaggggatg cctgcagtgc aggcgaacac cgggacattg atcgcgccga
     6181 ttctggctgg atcgattaca ggccgatcac cggtactcca tattcccacg tatcccgaat
     6241 actcacgggt ttgttggcct gtgcagaggg ctggttctgc acagacgtgt cagctcgcat
     6301 acctggcgtc gtgaggacca caccacgagc gcccacacga gttggcatgg agcgtacaga
     6361 ccggggggct gcccggctgc cgacatacga caggagggaa ggagttgtca gcactcctac
     6421 acaagacccg taccgggttc tttttcgcga tgatcggcat gctgatcatg tcgatgatgg
     6481 ccccgacggt tgcgttggcg aacgaggatg ctcagacctc gagcaaccag gctgtctcca
     6541 acgaaacggt cgtttccact gaatccagtg agtcgaccac gacgagtacc accgacgaga
     6601 atgcagtgac cgagcaatcg gccccagcca atccctggca ggagccgatt gactatggcg
     6661 ttgcgaacga caacgtgaag tgccgcaagg cagcgagcac gagcgcacgc gagatcacgg
     6721 tcatctacga gggacagacg atcgagatca caggtgtcga gatctgggcg gagggaatca
     6781 gctggacacc agtcaactgc gcgggcgttg gcgggttcat ttccactggg tacatctccg
     6841 tagagaccga gacggttgag gagcccgagg tccaggtcgt cgaggagacc ggcgtcgaag
     6901 agtcggtggt cgaggagacg gtcaccgatc agggcggtga gcagcaggta gaacagaccg
     6961 gcgtcgagca agtcgctcag gcgaacgcct cgcagaccga ggaagaggag acggacgaag
     7021 ccacctccga cgaggatcag gtcgaggacg ccatcgaaga gccagcaccg gttgaacaac
     7081 cggtgctgga gtcaatcgcg acaggatatg caacatccgc cgtcaactgc cgggtggccc
     7141 cgtccatcga ggcagaaacg atcgccggct tcgcagcggg taacgccgtg ccagtcacgg
     7201 gcgacgtgtt ctccacggag ggtctgaact ggaccccggt gaactgcgca ggcgtcggcg
     7261 ggtttgtcgc ctccgatttc atcacctttg aggcacccgc cccgaataat ccatcacaag
     7321 agtcgaccga gaacctggcg ggtgccagct cagtcgagac gaccgctccc gagacggcag
     7381 acgaacctgt cgaaggcaat accgtccccg acggttcagc cgatcaggct ctccagacaa
     7441 ccagtgagcc cacgggggac gcggcgcaag ctggcactga gaacatcgag gagccttcgc
     7501 tggccgaagc gatcgtcgag gatgtccaga gccgggttcc cgacgccgag cagatcatcg
     7561 acgagacgca gcagcaaatc gacgagtcgc tcggagaagc accggaggtc gaagagacgg
     7621 ccccggacgt cggtgacgag agcaacgcca ccgagggtgc tgaagagacg gctaccgacg
     7681 ttgaagacgg cggcgagccg accatcccgg atgccgacga gctcgccgat gagatcatcg
     7741 aagatgcaca ggagcaggtc gacgagtcgc tcggcaaccc gccggcaatt gcggaagcta
     7801 ccgaagaggc aaccgcggag gtcacggaag aggtagctga ggaagtggca aacaccgaca
     7861 cggcgaaccc tggaatgacc ggtcaggaag cgctcgatac ccagacgacg gacgagccgg
     7921 acgagccagt cacgacggaa gagacgatct cggaagccac ggaggacacg accgacgcgg
     7981 gaaccgaggg agccaccgaa gagcctcagg aggaggaagg cgagacccat gcgtctgcca
     8041 cgagcggtgt cgccgttgag gagaccgagt cgcgcactcc catcgaggac aacgccatca
     8101 tcggcaccgc cgacatcaac ggcaccggcg accaaggcct tccctgccgt gtatcgcctg
     8161 agatggacgc gccggtgatc accacgctgc ccgagaatat gagcgtcttc gtgctctccg
     8221 agccggacgc caacggctgg atgtcgatcg tctgtgccga ccgggttggc tacgcaccag
     8281 ccacttacct ctactccggc ggcgccaccc ttaccttcga tccggccgtg accgggaccg
     8341 cggtggtcaa cggcaccggc ggcggcgggc tcaactgccg cagtggtccg agcaccggct
     8401 acagcgtgat cggctggttt gccgagggcg cgacggtcac gctaaccggc aagtccagcg
     8461 gtgtctgggt cgaggtcatc tgtggcggca cacaggggtg ggcatacggc acctacctcg
     8521 gcggccacgg ctcgagcggt ggcgacacca ccagcgaccc ggacacgaac aaccaggcgc
     8581 cgtccagcgg cacggtgacg gtgagcggca ccggcggcgc gcggctgaac tgccgcaccg
     8641 gcgcgagcac cagcttcccg gcaaccacct cggtcgctga gggcaccgtg ctcaacgtcc
     8701 gcggcgcggc ttcgggcggt tggcttccgg tcgtctgcaa cggctccgac ctctgggtct
     8761 acacccagtt cgtgaccgcg ggtggcaccg ccaccccgca acagccgaag gaagagacac
     8821 cgagcaacgg cggcggaaca gatgccgtca ctggtcaggc cacggtggtc aacaccgacg
     8881 gtgcctcgct ccgctgccgc tccggcgctg gcctgaccca cagcacgatt accttcctgc
     8941 cagccggctc gaccgtctcg ctgcgcagtg gctcgcaagg cgactggacg gcggtggtct
     9001 gtgccggcca gaacggctac gcctacagca gctatttgag caccagcggt ggcggctcga
     9061 cggcggcacc cacgcccgag ccggccccga gccaaccgag cggtctcgcc aatggcgacc
     9121 acgccaggac caccgcaaac ctgaacctcc ggtacgaccc atacgccggt aacaacgtcg
     9181 cggcggtggc cccgcagggc accgtggtcc agatcaccgg cggacatgcc ggcaacaact
     9241 actacccggt caactgggac ggcctcaagg gttacatgtc ggccgactac ctggtcgaga
     9301 ccagcgaggc gctcagcgag cgcggcggct cggcgccaac gaccgactcc cagactgcgg
     9361 gcggcggcgg tggcacatcc acgggcaatg ctatcgcgga cttcgcgatg ggctacctcg
     9421 gatacccgta cgtctgggcg accgcgggcc cgtcctcgtt cgactgctcg ggcttcacct
     9481 actgggtgat caagaacgtg gtcggaatcg acatcggacg tggtctctgg acgcagatcg
     9541 ccgccggcac gccggtgagc cgcagcgcac tccagccagg tgacctggtc ttcttccaga
     9601 acacttacaa ggcgggcctg tcgcacgtcg gcatctacat cggtggcaac cagttcgtcc
     9661 attcccaaaa cgaggcgacg ggtgtggtaa tcagcgatct cgattcgagc tactacggtc
     9721 cccgctggta tggagcgcgc cgcatcggct agcgagcccg cccaacgaga gggacacgaa
     9781 gaggaccggc cgggagcagt tgctcccggc cggtttcgtt atgcttgcca caggaggatt
     9841 cctgccccga tgccctcgcc caacgatctc gatcatctct cccttctgct ccagcgtacc
     9901 gttcccgaca tcggcccagt ggtcggactc gagcacgtcg ccgagggcta cggcgctacc
     9961 acgctcagag cactcggcgg gctgtgcatc cgtatccccc actctgccca ccttgccttg
    10021 aagcagaacc gtaccgccac cgcgctccac cgcctggcac cgttgctccc ggtgccgacc
    10081 ccggtcccgc tatggacgct gcccgtcggc taccccttcg agaccggtgc gatcggctac
    10141 cagtggatcg atggcgagca gccggaccct gagacgtccc ctccgtcact cgccgagcag
    10201 gtcggccgct ttctcgcgac cctgcacggc atcgacggcg agacgctcga cacattcgac
    10261 gggcgcgttc ccggcccggc tgatgttgct gccgaacgtg accaggtgat gggcgtcgcc
    10321 ctcccatggc tatgggagcg acagtcggca ggcgtgatgg attggattga gcgctggtgg
    10381 gcatccttcc gcgcagagcg cgaagctgcc cgctatgagc tccgcctcgt tcacggtgac
    10441 ttctggcacg gcaatctgct ggccgacgca tcacaggagc gcctcaccgg ggtgatcgac
    10501 tgggagcaga tcgcgctgga cgaccaggca caggacctcg ccaccctgct ccacggtggc
    10561 acagcgttca gtcacgccgt gctggccacc taccgggcgg caggtggcac ggtggacgcc
    10621 gcaaccatcg cccggcggga ctggctctgg ggctaccggg aattcaccgg catcgcactg
    10681 gctctcgagt tcggtgacga ggccgaggca ctcgacggca tgcacaagct gcagcagggt
    10741 gccctcaggc atctgttcat caccggttcc gaaccgtgaa ccacagcgag ctgcgatcgc
    10801 accgatcgac catcgacgcc gtcacgctgg cgtggtacga gcacggcggc gagcttggca
    10861 gcccaatcgt ctgcctgcac ggactcggag ccagctccct cgacttcgcg tggttggcca
    10921 cctggccagc ctttcgccat atgcgcctgc tgctgatcga cgccgccggg cacggtgcca
    10981 gcgaccgccc tgacgactgg ccctactcca tcgaagccca cgccgagctc attgtcgggc
    11041 tcctgagaga gatcgccggt acaccggtcg ccctcgtcgg ccacagcatg gtctgctcga
    11101 tggcgatcgc cctcgctcac cggcacccag ggctggtgga ccggctcgtg ctggccgagc
    11161 cgaacctcga tccgggtacg ggaacgctga gcggccacgt cgcgcgacag cgagaggagc
    11221 ggttcgtgac gcggggatac gcggcgctgg tttaccagac agagcggacc gcagcgcgcg
    11281 gggatctcgt cgccgcaggc ttcctgcagt cgctgcggct cgcctcaccg atggtgatcc
    11341 accggagcgc cgtctccctc cgcgccgaac ggttgccaac cttccacgag cagctggaaa
    11401 cgatgacgct gccccgaacg ctgctccggg gcaagcggac tccgccactc gctccacccc
    11461 tctcgaccac tgccatccag caagtcgtca tccccggtgc cgggcaccag atgatgatcg
    11521 acaaccctga cgccttcgcc acgtcgattc gcacagcgat tgacgagtag gaatccttgt
    11581 agggtcagct tcccgcaaca gtgcccacta ttgtcagtgg gaattgaacc cgcccttccg
    11641 gtttcaatcc ccaatcgcgg cattctgagc acagtgagga gtttccacgc gaagcaccgg
    11701 cgcggcgccg ctttcaacgc ttggggagtc cggccagatg ctgtctgcag cagcacgcct
    11761 cgcccgagga tacttcgact atggcaaacc cgcccggtgc gaggatcgcg accgggcggg
    11821 tgtctctgcc acatgggttg ccgccagcga gattactcct cggcgttgtc gacggcagcg
    11881 agcacaccgt cctcgtcggc ctcgccatcc ttggcgccca ccgtgatgcg ccccgacaac
    11941 tccggcgact tcgccttgat ctgctcgcgg atgtcctcca gcacatcggg gttggacttg
    12001 aggaagagct tcgcgttctc ccggccctgc ccaagacgct cctcgtcgag atagaaccac
    12061 gcgccgctct tgcggacgat gccgaggtcg gtcgcgacat cgagcacgct gccggaaacc
    12121 gagatgccct cgttgtacat gatgtcgaac tcggcctgcc ggaacggcgg agcgaccttg
    12181 ttcttgatca ccttcacccg ggcacggata ccgatggcgt cagtgccctg cttcagggtc
    12241 tcgatgcggc ggatgtccat gcgcaccgag gagtagaact tcagcgcatt accgccggtc
    12301 gtggtctccg ggctgccgaa catgacgccg atcttcatgc ggagttggtt gatgaagatc
    12361 acgcacgtct tggagcggct gatcgcaccg gtcagcttgc ggagtgcctg gctcatcagc
    12421 cgtgcctgca gtccgacgtg ggagtcgccc atgtcacctt cgatttcggc cttcggcacg
    12481 agcgccgcca ccgagtcgac cacgatcacg tcgagcgcgc ccgatcgaac cagcgtctcg
    12541 gtgatctcca gcgcctgctc gccggtatcc ggctgcgaga tgaggagctc gtccagatcg
    12601 acgcccagcc gcccggcgta gaccggatcg agcgcatgct cggcgtcgac gatcgccgcc
    12661 aagccgccca tcttctgcgc ctcggcgatg atgtgcagcg cgagggttgt cttgcccgag
    12721 gattccggac cgtagatctc gacaatccga ccgcgcggca cgccaccgat gccgagcgcc
    12781 aggtccagcg cgatgcttcc ggtcgggatg gcatcgacct gcatcgggtt gacctcgccc
    12841 ggcttcatct tcatgatcga gcccttgccg aagttgcgct cgatctgcgc gatcgccatg
    12901 tcgacagcgc ggtcgcgatc tgcctgtggt gctgccatgc tcattgacct ttcacctgcc
    12961 tggcctttcg gcgatatccg gagctcccct gtggcgcttc tgcgctctga cgcggcattc
    13021 ggacctagcg ccggtgctgc gcacccacgg ccagaaaggg cgaaccggtc ttcggattct
    13081 agcgcgtttc gcgacggaat gtatcattgc ttccgccgcc ccaaaatacg aacattcgtt
    13141 cgtatttcac actgccaagt gtacggcagc gcttcctttc agacaaccgc tgaggggagt
    13201 tcgagagtgt gccaggcacc ctccctgcct ccactctttc ggccctgccc tgagccgtcc
    13261 ctggctggct ctaccgcggt atactgcagc cacatcccgg catgtcgcgg cggagcaaac
    13321 aactcgccat catggcaggt acgggctggt gatggggtga cctcggggag cggcaacagg
    13381 agaactcctg tccgtagtgg catggatgtt gcacttgttg accccagaca acccgagaga
    13441 accgtttcga atccgagtgc aacgggaggg aggtagcgat gtacatcggt ggtggactgc
    13501 ttgtcatcat cctgatcatc atcattctga tcctgatcct ctaaatgacc cggatcagac
    13561 caatacgcca ctgacgctcg gccagcaggc cggaccgtcg ggcgatcaga ccggtaccgc
    13621 tgctggcagc agtaccggtc tttttgcgtc tacccctccg tgtccgcggc gatgcggaaa
    13681 cccatgttgc cggttgagct gtccggcgtg ttcgaggtcc gtgccgcgac ccgatagcga
    13741 ttgcagtagg acttgtgaca gaggtagctc ccaccacgca tcacgtgcgt gctcccccgc
    13801 tccggcccga ttggatcgac acgatgctcc gtcgggtgcg gcccgggact ccaccaatcc
    13861 gcgcaccact cccagacgtt ccccgcgacg ttgaacagcc cgaacccgtt cggctcgtag
    13921 gcatcgaccg gtgcggtatc gaaatagccg tccgctgtcg tgttgcggtc cggaaatctg
    13981 ccctgccaga tattgcagca gtgcttgccg ccgggcatca gctcgtcgcc ccaagggaag
    14041 cgcttctggt cgagcccacc ccgcgcggcg aactcccact ccgcctcggt cggcagccgg
    14101 gtactgctcc aggcgcagaa cgccatcgca tcgttccagg agacatgcac cactgggtgg
    14161 tcaaggcgat catcgatggt cgcgcccggc ccctcgggag cgttccagct ggcaccgtcg
    14221 ataccggtcc accatggcgc agcaccggat gccccgcgca ccgtgacccc gcgcttcggc
    14281 atgaacagat ggaaaacaaa tgaccacccg aagcgctccg cctcggtgac atagccgctc
    14341 gcctcgacaa acgctcggaa ctggcggttg gtgacggcat agcggctgat ccgaaacgac
    14401 gagacactga ccggccgcac tggtccttcg ccgtcagtcg gaaagcccgt cggatcgttc
    14461 gtgcccatgc agaaggtgcc gccgtcgagc gcaatcaggt ccgatcggac gatcccgtgg
    14521 acctccgcgc gaacctcccc gtttggtagc tcttggaata cgtcgccagt catggcgtcg
    14581 aactcccgtc ccggcacgca gcagcgtcca cgctcaatcg ccatcaggac ccccgttcgc
    14641 tcgtgccctg cgtcgcctac tgcagccgcc ctcgtgccag ggtcggccag acggcctcca
    14701 cgcagccagc ttcaaccacg aagtgctgct cgtgcaggtg gacggcctgg tcgctgtagc
    14761 cgatgttgag ccgcaggcga tctccgacct gtacgtcgca gttcggcccg tcgaggcgga
    14821 tggttccgtg ctcggcagag aacgccagct gcgacacgcc ctccaggcca atcacctccg
    14881 gcacaagatt ggacggatcc accgacttcc gcccggcgtc gaagatgatc cggtcgcgtg
    14941 ccggtcgact ggtaaccgtc accagcagcg agagcgccgg ctgcaccggc gctccgaggt
    15001 cgcgatagaa gcggtccccg aaaatcccgc caccagcctg cacctcggtg atgccctcga
    15061 tgcccgccgt gaccaggaac gtgcccgtgc cgccggccga tacaatcggc acctcaatgc
    15121 cggccgcgcg aatcgcctcc accgtctcga gtactggcgc cagcgcctcg cggatcgcct
    15181 gcgctcgaac ctccggatcg ctgaccttga gcgtatgccc ctcccacgac atcacacccc
    15241 cgaagcgaac atgcgacatc ccggcgatcg ccttcgccag ctcgaccgtt gcctcgcctg
    15301 gctccgtgcc cgcgcggttc atgccacagt tgacttcgat gatcagacgc ggtgaggtac
    15361 cggcctcggc cgcggccaca tcgtgttcct tcgcgttctc gatgctgtcg acggcgacag
    15421 caatgtccgc ctgccgggct aggtgcgcga gacggcgggt cttgatcggc ccgaccacct
    15481 gattggcgat gaggatgaca cggatgccgg aggcaacata gacctctgcc tcgctcgtct
    15541 tggcgcaggt gatgccatgt gcgccggact cgatgagcaa atgggctacc gccggcgact
    15601 tgtgcgcctt cgcgtgaggt cgccagccgg tgccgtgctc gcgcagcgcg gatgccatgg
    15661 tctcgatgtt ggcgtggaag gcagcacggt cgatgagcag agccggag
//
LOCUS       DASYWL010000128         2042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_475326, whole genome shotgun sequence.
ACCESSION   DASYWL010000128 DASYWL010000000
VERSION     DASYWL010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475326"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            625..1182
                     /locus_tag="VGR22_04545"
     CDS             625..1182
                     /locus_tag="VGR22_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127870.1"
                     /translation="MRSRLPAIVLAAFSLTLGVRAGPVVVSGQEGATPSASPEPVVVH
                     PANIYTGTCEDLDESAYQLESVTTEPVEVEGTLGASPVPAETGLPGDVIGRSVSQVDA
                     SLQELLEGQYVVNVYNSENMETAIACGAIEGEIETESLSIVLHEVNDSGVVGQATFTD
                     HGDGTAGVTVTLLDEEAELYATPQG"
     gene            1445..1981
                     /locus_tag="VGR22_04550"
     CDS             1445..1981
                     /locus_tag="VGR22_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127871.1"
                     /translation="MFGAILAIISLMVGLTASATLVSAQNATPTGEPPAAHPAHIHSG
                     TCEQLGDIVYPLEDVTGAALEGTPDASPVPVDQAGASNVLARSQSTVDTSIDDLLTEG
                     HAINVHESAENIGTYIACGNIEGEPDEDGSLIVELQELNQSGIQGQAVLTPNDEGTLS
                     VTITLVDAEGATTATPAS"
BASE COUNT          376 a          638 c          670 g          358 t
ORIGIN      
        1 atgctctcgg cctcatcgcg ttgtgggtca tcctggccgg ccttacgctc cagctcggcc
       61 tcctgctgcc cgtaggcctc ggcggcgtcc ttgtagggct ggtcgccctc ttccttgcga
      121 tcacggtgcc gatcttgagg ataggggtag gacatgcggg tcctcctgtt gtcagaatcg
      181 ttaggcccac cgcatggcgg ccatccatca ggttcctgca cgagctatgc cacgcaggcg
      241 ggtgcaaagt gctcatcgga ccgtgtaccg aagcgtcatg gcggagacga cgcccgtcac
      301 gacagcggag cccagctggt cctcgttgtt ggcccacacc acacgccaat gacgaccgcg
      361 gcaaccagca gcacgacaac caacacgatg attgcccagc gggactgcat gaaacatgtc
      421 ctttcgaccc gattgatccc gggcggtggg gtacccgata tcgggccgtt acctccgccg
      481 tagcgatgtc gttccccacg caagacactg gccgcggcct tccatcagcc gacccgcagg
      541 tggcacgtct cctgcgtgga gccttttcaa gaatgacgtc ggctcctgcg ggtctttcga
      601 gttgatcctt caggaggttt tgcgatgagg tcgagactcc ccgcaatcgt actcgctgcc
      661 ttttcgctga cgctcggcgt ccgggcgggc cccgttgtcg tctctgggca ggagggcgcg
      721 actccgtcgg cgtcgccgga accggttgtg gtgcatccgg ccaatatcta cacgggcacc
      781 tgcgaggatc tcgacgagag cgcctatcag ctcgagagcg tgacgacgga gccggtcgag
      841 gtcgagggaa cgctgggcgc gtcgccggtg ccggcggaga cgggactgcc tggcgacgtc
      901 atcgggcgca gcgtctcaca ggtggacgcc agcctgcagg aactgctcga ggggcagtac
      961 gtggtgaacg tctacaacag cgagaacatg gaaaccgcga tcgcctgtgg cgcaatcgaa
     1021 ggcgaaatcg agacggagag tctgagcatc gtgctgcacg aagtgaacga ttccggcgtg
     1081 gttgggcagg cgaccttcac cgaccacggt gacggcaccg ccggcgtgac ggtcaccctg
     1141 ctcgacgagg aggcggagct ctacgccacg ccgcaggggt agccataggc catcgcaggc
     1201 gagcgctggc gcgtgcgtct tccgaagcaa tctgtggctg gatcccaagc cgataaacgt
     1261 tgacagcggc gccccgccaa ccggcacggg gcgctgctgt ttcgcttgcc ggtgtagcgg
     1321 ccagtgcggt ggcgggtggc acacggcatg cgattgcgaa tggacggcgc agcgctgcgc
     1381 gcccgtctac cggttatccg cttgtcgata gatagttgga gggtcaacaa ccatgagaag
     1441 gcgaatgttc ggagcgatcc tggctatcat ctccctgatg gtcgggctaa cggccagcgc
     1501 gacactggtg agcgcgcaga acgccacccc tacaggggaa cccccggccg cccatccggc
     1561 ccatatccac agtgggacgt gtgaacagct tggcgacatc gtctatccgc ttgaggacgt
     1621 caccggcgcg gcgctcgagg gcacgccgga cgcgtctccc gttccagttg atcaggctgg
     1681 agcgtcgaac gtgcttgctc gcagccagtc aacggtggac acatccatcg atgatctgct
     1741 gaccgagggg catgcgatca acgtgcacga aagcgccgag aacatcggca cctacatagc
     1801 gtgcggcaat atcgagggtg agccggacga ggacggctcg ctgattgtcg aactccaaga
     1861 gctcaaccaa tcgggcattc agggtcaggc ggtgctgaca ccgaatgatg agggcacatt
     1921 gagtgtcact atcacactcg tcgacgcgga gggagcgaca acggccaccc cggcgtcgtg
     1981 aggtggcgat cccgattgac ggccgccgat gcgggttgct gactctgcga ccacccaccg
     2041 at
//
LOCUS       DASYWL010000129        11479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_419250, whole genome shotgun sequence.
ACCESSION   DASYWL010000129 DASYWL010000000
VERSION     DASYWL010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 11479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11479
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419250"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            309..1424
                     /locus_tag="VGR22_04555"
     CDS             309..1424
                     /locus_tag="VGR22_04555"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEV2127872.1"
                     /translation="MAEAIIATGAVARKIGEPIGLERVVVPAPGPGEARVRILASGVC
                     HTDLSAKNGVFGPDVVPLLLGHEGAAVVEDVGVGVSNVRAGDYVILAWRAPCGGCRFC
                     EAGKPHLCAASLNATQKMTAERDGIELNPVLGLGTFSTHTLVHAGQCVPVREGLPSAQ
                     MSLIGCGVMTGVGAALYTGKVEPGSSVAVFGCGGVGDSIIMGSVLAGATAIIAVDVDP
                     KKLEWARGFGATHLVNAMEVDPVEKIKEITGGHGVNFSFEAVGRAETTMQALLARDLA
                     GTCVVVGVAGPGSTIEQVPLGKLFDLGGTIRFSWYGDCLPTRDFPMLASWYEQGRLDL
                     DDVVTKKIALDDVEDAFHAMERGETLRSVIMFDEAEA"
     gene            complement(1523..4213)
                     /gene="mutS"
                     /locus_tag="VGR22_04560"
     CDS             complement(1523..4213)
                     /gene="mutS"
                     /locus_tag="VGR22_04560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEV2127873.1"
                     /translation="MSTTKTGKASDSKVVPLRQQYLQIKARYPDTILFFRLGDFYETF
                     DDDAKIAAEVLDIVLTGREMGKDLRVPMAGIPYHSADGYIARLIAAGHKIAVCEQIGE
                     TTRGRGLVERDVTQVITPGTVVDPSMLDARQNNDIVGLAIDGKRAGIAFADITTGEFR
                     TTQITADDEEELLLAVGRELLRLHTAEVVMPADLTDSLSLPMASWLPEGASTSRSETW
                     RWQEERAAEALMRHFGVDSLDGFGTGGKPLATRAAGGLLQYLEETQLSGLRQITSLAT
                     YSTAGYMTLDAQTIRNLDLSESQRGDRRHSLTAVLDRTETPMGARLLRRWIRQPLLDV
                     EELEARLDGVEFLVNDASCRARLIELLDKVKDIERLVNRALIGNATPRELGTLRDTLA
                     VLADIGPIGQQVPHARPIPAHSDLAELLESALVEDPPVNWGKAQVIRSGFASDLDGYR
                     LKASEAREWIANLERTERERTGIKSLKVGYNRVFGYYIEVTTAALASADRDRMAAGES
                     GGALPEEYIPKQSLTNATRYFTPELKEYETLVLNAEGTLSSMEQDVFHRVMRQVAEQA
                     GKLLETAAAIAWLDTIAALADVAARNSYVRPEIDESPVVEISAGRHPVLETTLPAGEF
                     VPNDARLDSDDDQIIILTGPNMAGKSSWLRQVALIALMAQIGSFVPASRARIGLVDRI
                     FTRIGAQDNIATGQSTFMVEMLETANILHHATPRSLVVLDEIGRGTSTYDGLAIARAI
                     VEHLHNASHLGCKTLFATHYHELTELAGVLPRVRAFRMEVLEEGDQVIFLRQVVPGGA
                     DRSYGIHVAQLAGLPRAIVRRASEILTDLESNGHGDQQPGTRRQAMAQSAPIEPANLQ
                     LTFFNEEPAVVGRLRNLDVESLSPLEALNLLYELKREVTP"
     gene            4399..4686
                     /locus_tag="VGR22_04565"
     CDS             4399..4686
                     /locus_tag="VGR22_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEV2127874.1"
                     /translation="MALYAATLTYTEDKDKIQEVRPTHREYLQKLVESGKLHESGPFT
                     DDSGSLIVYRVESEAEARELLANDPFTTSGVITDATVKEWKIVMSSVGTAL"
     gene            4729..5097
                     /locus_tag="VGR22_04570"
     CDS             4729..5097
                     /locus_tag="VGR22_04570"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2127875.1"
                     /translation="MTVIHGIVVDCADPEKVSTFWQAVLGWQYRRKADDWHSLANPDG
                     NPHLSFGTVPEGKTVKNRVHLDIRPVDRSMEEERERLEALGAATLRLVDENPDDVHYM
                     MADPESNEFCLLSPDLDRVE"
     gene            5159..5437
                     /locus_tag="VGR22_04575"
     CDS             5159..5437
                     /locus_tag="VGR22_04575"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEV2127876.1"
                     /translation="MTLYAALFTYVADPARIDEVRPAHREYLKQLLDEGTLHEAGRFG
                     DERGGLIVYNTESESDARTLLDRDPFSTSGIISIDSVQEWKVILSACT"
     gene            complement(5530..6549)
                     /locus_tag="VGR22_04580"
     CDS             complement(5530..6549)
                     /locus_tag="VGR22_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2127877.1"
                     /translation="MNDEMGNYGVAKIDDLVKDNMNSDDKVEVPEEIQTQMRAGEDEV
                     EQEADLERDLDVLQKLDPNFINDPVRLYLREIAETPLLTHDQEIDLAKRVENGDLTAV
                     QQFVRANLRLVVSIAKRYVNRGLTLLDLIQEGNIGLMRAVQKYDWRKGFRFSTYATWW
                     IRQAITRAIADQSRTIRLPVHMGDSISKYRKTLNLLAQDLGRQPTPEEVAEAMSVPPE
                     KIHQIVQAAQRTISLETPIGNEDDTSLGDLIADEVSETPYEAASESMLKRDVSAALDT
                     LSAREKLVLQLRFGLGQGHQHTLAEVGEQLQISRERVRQIENEALQKLRRLDGDRLFA
                     YHQEL"
     gene            complement(6994..7395)
                     /gene="erpA"
                     /locus_tag="VGR22_04585"
     CDS             complement(6994..7395)
                     /gene="erpA"
                     /locus_tag="VGR22_04585"
                     /inference="COORDINATES: protein motif:HMM:NF010147.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEV2127878.1"
                     /translation="MEEMIRMITMTSEAVSQLREFLDEQGTPDHALRVFVAPGGCSGL
                     QYGMTIDEQPDEGDEVVETEGVRVLVDSFSAMYLEGAEIDYVNSLMGGGFTVSNPNAV
                     AGCACGHSFDAGGNAGTAQGCGTGVASAGRV"
     gene            7581..8600
                     /gene="rsgA"
                     /locus_tag="VGR22_04590"
     CDS             7581..8600
                     /gene="rsgA"
                     /locus_tag="VGR22_04590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEV2127879.1"
                     /translation="MGKRRRRGGGQDRQDQAAAAIEQLVEREAPPEAMRGVVVRAHGL
                     WYEVRTHDPEATWIATMRGVLKKKARRTDIVAVGDHVWITDAGDGEAVIERVESRKSV
                     FARTARNTRDTEQVILANPDQVLIVFAAREPEPHLRMLDRFIILAERHDLSVRIVVTK
                     TDLEQNLGSNDPLGPAEKRFALYESIYPVHYVSSVTGEGLEELREELFGHISVVAGPS
                     GVGKSTLLNALDPRHERETGEVSEATGKGRHTTIGAQLYEIGPDTYIADTPGMRALAM
                     HAVPEEELEWCYREFREYLGECRFSGCTHMHEPGCAIREAVERGEIPEDRYESYVMVR
                     QESEG"
     gene            8738..9103
                     /locus_tag="VGR22_04595"
     CDS             8738..9103
                     /locus_tag="VGR22_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127880.1"
                     /translation="MQGHIGKGGVTVEDIARDMEDVIDDLLQRIEGEAFTTTQFIELL
                     RSVPEGEAAYEAAWRRWGEQEHESKMVIHGQVIPLVLRRSRFARWDGYAHGEDDAYAV
                     PAWWVLVSSGDAPKDTGLR"
     gene            9291..11423
                     /gene="ftsH"
                     /locus_tag="VGR22_04600"
     CDS             9291..11423
                     /gene="ftsH"
                     /locus_tag="VGR22_04600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEV2127881.1"
                     /translation="MPQKDQETSQFSMRRPDQDGNRGRRPQPGNDGRPDPDMPERRSP
                     RIPTWVVATLIIALVGWYIWQFFGPQDEGDALDVPYTTVTEQLVEGNIERAVLSETSI
                     AAQLESPILWNTDNEQVDPDAVADAPGIVETTEIEATLPQGVEIPDLLPALQEADVVI
                     EGEAAGGTIWTSILISFFPFLLILGLILFMGRQMSRGQQNVFGFGRSKARQNDPERPQ
                     VTFDDVAGEDEAKRELMEVVDFLSNPAKYHALGARLPRGVLLVGPPGTGKTLTARAVA
                     GEAGVPFFSVSASEFVEMFVGVGASRVRDLFDKAKAASPAIIFVDELDAVGRQRFAGL
                     GGSNDEREQTLNQLLVEMDGFETNQEVIVMAATNRPDVLDPALLRPGRFDRQVTVGLP
                     DREGRLAILKIHTRGIPLSASIDLEQIARSTTGFSGADLSNLVNEAALTAARRNSKDI
                     RKEDFDEALDKILLGVAKSGLTNPKEREVVAYHEAGHALVAHFTPGSDPLRKVSIVPR
                     GRALGVTVQMPDEDRHNYSRQYLLGRLAMMLGGRAAEMVVFDEITTGAENDLKQATSL
                     ARRMVGLWGMSDAVGPLYLGTGEEHVFLGREITQDKSYSDATAEKVDAAVRGIVENAL
                     REAVDLTSQYREQLDGMVRALLEKETLDAPEVIEIFGPAVDEDPNVGIDDDEGLSARQ
                     PRAEDGRQTAGTEDGTIVRRKVGDSGAS"
BASE COUNT         2007 a         3500 c         3897 g         2075 t
ORIGIN      
        1 atcatcatcg gtcgcctcgt gcgcgagagc tggcgccgct atcggtggga cacccgcccg
       61 acacgtcgcc cgtaacctgt tggcgcaagc tcctaacagg cgttttcagt tcaggagggg
      121 tgacgacagc cgggcgctag cgcccggctg tctgcgttgc cccagaagcg tccacacttg
      181 gcagtgccac cactggtcaa ccactcgcat gcgcctcaac caggtcccgc ggtgcattgc
      241 caagcccgtg cacgattact agaatgccat gagagcgggt tctcacattg caggaaaggg
      301 tgtgtggtat ggctgaggcg atcatcgcga caggggcggt cgcgcggaag atcggcgagc
      361 ctatcgggct cgaaagagtc gtcgtgccgg cgccggggcc cggcgaggcg agagtgcgga
      421 tcctcgcaag cggtgtatgc cacaccgacc tctccgccaa gaacggcgtg ttcgggccgg
      481 acgtggtacc actgctgctc ggacacgagg gggccgcagt cgtcgaggac gtcggggtgg
      541 gggtgtcgaa cgtcagggca ggcgactatg tcatcttggc gtggcgcgcg ccctgcggcg
      601 gttgccgatt ctgtgaggct ggcaagccgc atctctgcgc ggcgagtctg aacgcgacgc
      661 agaagatgac tgccgaacgg gacggcattg agctcaaccc ggtgttgggg ctcggcacct
      721 tctccacgca cacattggtg catgcgggcc agtgcgtgcc cgttcgggag gggctgccgt
      781 ccgcgcagat gagcctgatt ggctgcggcg tcatgacagg cgtcggagcc gcgctctaca
      841 ccggcaaggt cgagccggga tcgtccgtgg ccgtgttcgg ctgtggtggt gtcggcgact
      901 cgatcatcat gggctcagtg ctggcgggcg cgacggccat catcgcggtc gatgtcgatc
      961 cgaagaagct ggagtgggca aggggcttcg gggcgacgca tctcgtcaac gccatggagg
     1021 tcgatcccgt cgagaagatc aaggagatca ccggcgggca cggcgtcaac ttctcgttcg
     1081 aggccgtcgg ccgggcggag acgacgatgc aggcgttgct cgcccgtgac ctcgccggca
     1141 cctgcgtcgt ggtcggcgtg gcaggcccgg ggtcgacaat cgagcaggtg ccgctgggca
     1201 agctgttcga tctcggcggc acgatacggt tcagctggta cggcgactgc ctaccgacgc
     1261 gagactttcc gatgctggcg agttggtatg agcagggccg gctcgatctt gacgatgtgg
     1321 tgacgaagaa gattgcgctc gacgacgtcg aggatgcgtt ccacgccatg gagcgcggcg
     1381 agacgctccg atcggtcatc atgttcgacg aggcagaggc gtagcgacgc cgtaggggcg
     1441 agtatcgcga gtcgcctgag aggacatacc ctccgggagg gccagggttc atctctacag
     1501 atccgtgcga ggacaggggc ggctacggcg tgacttcgcg cttcagctcg tagagcaggt
     1561 tgagcgcttc gagcggggag agcgactcga cgtcgaggtt ccgaagccgg ccgacgactg
     1621 ccggctcctc gttgaagaag gtcaactgca ggttcgctgg ctcaatgggt gctgattgcg
     1681 ccatggcctg ccgccgcgtg cccggttgct ggtcgccgtg accgttcgac tccaagtcgg
     1741 tgaggatctc cgacgcgcgg cgcacgatcg cccgtggcag gccggccagc tgggcgacgt
     1801 ggatgccgta ggagcggtca gcaccgccgg gaacgacctg tcgcaggaag atcacttggt
     1861 caccctcttc aaggacctcc atgcggaagg cccggacccg cggcagcacg cccgccagct
     1921 ccgtcaattc gtggtagtgc gtcgcgaaga gcgtcttgca gccaaggtgg gaggcgttgt
     1981 gcaggtgctc gacgatggcc cgggcgatgg cgaggccatc gtaggtgctg gtgccccgac
     2041 cgatctcgtc cagcaccact aggctgcgcg gcgtcgcgtg gtggaggata ttcgccgtct
     2101 cgagcatttc gaccatgaat gtgctctggc cggttgcgat gttgtcttgg gcgccgatgc
     2161 gcgtgaagat gcggtcgacg aggccgattc gcgcccgcga cgccggtacg aacgatccga
     2221 tctgcgccat cagcgcgatg agtgccacct gccgcagcca actgctcttt ccagccatgt
     2281 tcggtcccgt caggatgatg atctggtcgt catcactgtc gagccgggca tcgttcggca
     2341 cgaactcgcc ggcgggcaag gttgtctcca gcaccgggtg gcgtccggcg ctgatctcga
     2401 cgacggggct ctcgtcgatt tccggtcgga cgtagctgtt gcgagcggcc acgtccgcca
     2461 gtgccgcgat tgtgtcaagc caggcaatgg cagcagcggt ctccaggagt ttgccagcct
     2521 gctcggcgac ctggcgcatg acccgatgga agacatcctg ctccattgag gagagcgtcc
     2581 cctcggcatt gagcacgagc gtctcgtact ccttgagctc gggtgtgaag tagcgcgtcg
     2641 cgttggtgag agactgtttc gggatgtatt cctccggcag ggcaccgccg gattcccccg
     2701 ccgccatgcg gtcccggtca gccgatgcga gggccgccgt ggtgacctcg atgtagtagc
     2761 cgaagacacg gttgtagccg accttgagcg acttgatgcc ggtgcgctcg cgctccgtgc
     2821 gctcgaggtt ggcgatccac tcccgcgcct ccgacgcctt gaggcgatag ccgtccaggt
     2881 cgctcgcgaa gccgctgcgg attacttggg ccttgcccca gttgaccggc gggtcctcca
     2941 ccaaggcgct ctcgagcagc tcggcgaggt cactgtgggc tggaatcggc ctggcgtgcg
     3001 gcacctgctg gccgatcgga ccgatgtctg ccagcacggc gagtgtgtca cgcaaggtgc
     3061 cgagctcgcg cggcgtggcg tttccgatga gggctcggtt cacgaggcgc tcaatgtcct
     3121 tcaccttgtc cagcaactca atcaggcggg cgcggcaact tgcgtcgttg accaggaatt
     3181 ccacaccgtc aagtcgggct tcgagctcct cgacgtcaag cagaggctga cggatccagc
     3241 gccgcaacag gcgggcaccc atcggcgtct cggtgcggtc cagcacggcc gtgaggctgt
     3301 gccgcctgtc gccccgctgg ctctccgaca ggtcgagatt gcgtatggtc tgcgcgtcaa
     3361 gcgtcatata cccggcggtg gagtaggtgg ccagcgaggt gatttgccgg agcccggaga
     3421 gctgggtctc ctcgaggtac tgcagcagcc cacctgcagc gcgggtggcg agcggttttc
     3481 cgccggttcc gaaaccgtcc agcgagtcga cgccgaagtg gcgcatgagt gcttcggcgg
     3541 cgcgctcctc ctgccaacgc catgtctcac tccggcttgt actggcgccc tccggcagcc
     3601 acgacgccat cgggagcgag aggctatccg tgaggtcggc cggcatcacc acttcggcag
     3661 tgtggaggcg cagcagctcc cgaccgaccg cgagcagcag ctcttcctcg tcatcggcag
     3721 tgatctgggt ggtgcggaac tcgccggtgg tgatatcggc gaaggcgatg ccggcgcgct
     3781 tgccatcaat cgcgagcccg acgatgtcgt tgttctggcg ggcatcgagc atcgacgggt
     3841 ccaccacggt gcccggcgtg atcacctgag tgacgtcgcg ctcgacaagt ccgcggcccc
     3901 tggtcgtctc gccgatctgc tcgcagacgg cgattttgtg cccggcggca atcaggcggg
     3961 cgatgtagcc gtccgcagag tggtagggaa tacccgccat cggcacgcgg aggtccttgc
     4021 ccatctcgcg accagtcagc acaatgtcaa gcacctcggc ggcgatcttc gcgtcgtcat
     4081 cgaaggtctc gtagaaatca ccgaggcgga agaagaggat ggtgtcaggg tagcgcgcct
     4141 taatctgcag gtactgctgc cgcagcggca cgaccttgct gtcggacgcc ttgccggtct
     4201 tcgtcgtgct catggggaat cgcatgcctt gctgtgatgc cggagcgacc ggttcagatc
     4261 gttcgtacac gtgaaagagt accaagccgt cggcactctt ccaaccggac agtgtgaccg
     4321 gtggcaggtt cacgaaatgg gcgagtgccg gtatgctgga ccggtaagca cggaatcgaa
     4381 aaagcagggg gatcgatcat ggctctctac gcagcaacac tgacctacac agaggataaa
     4441 gacaagattc aggaggtacg accgacccac cgcgagtacc tccagaagct cgtggagagc
     4501 ggcaagctgc acgagtccgg gccgttcacc gatgacagcg gctcgctgat cgtctacagg
     4561 gttgagtcgg aggccgaggc gcgtgagctg ctcgcgaacg atccgttcac gaccagcggc
     4621 gtgattacgg atgccacggt caaggagtgg aagatcgtga tgagcagcgt cggcaccgct
     4681 ttgtagcagg gcacggccgt ccctgcgagt gccccggagg tacacgacat gacggttatc
     4741 cacggcattg tcgtcgactg tgctgaccca gagaaggtga gcacgttctg gcaggctgtg
     4801 ctggggtggc agtaccggcg caaggccgac gactggcatt cgctggcgaa cccggacggc
     4861 aatcctcacc tgagcttcgg cacggtcccg gaggggaaga cggtcaagaa ccgggtgcat
     4921 ctcgacatcc gacctgtgga ccgaagcatg gaagaggagc gagagcggct cgaggcgctc
     4981 ggtgccgcga cgctgcggct ggtcgatgag aatccggacg atgtccacta catgatggcc
     5041 gatccggaaa gcaacgagtt ctgccttctc agcccggacc tggacagggt tgagtgatgc
     5101 ttcgtccggc gttgtccgta tggccatgcg gacatgtcgt gtacagaggg agatcaccgt
     5161 gacgctttac gccgcactct tcacctacgt tgccgacccg gcgcgcatcg atgaagtccg
     5221 cccggcccac cgcgagtatc tcaagcagct gctggacgag ggcaccttgc atgaggctgg
     5281 ccgcttcggc gatgagcggg gagggctgat cgtttacaac accgagtcgg agtccgacgc
     5341 gcggacgctg ctcgacaggg atccgttcag cacgagcggc atcatctcca tcgactcggt
     5401 acaggagtgg aaggtcatcc tcagcgcctg cacgtagaaa gcgtttcggt attcgcccaa
     5461 agaacgtgaa gacccccggt gtgatcacac ccgggggtct tgtatcaggc tgttccaggc
     5521 tgcgagagcc tagagttctt ggtggtaggc gaagaggcgg tcaccgtcca gacggcgcag
     5581 cttctgcagc gcctcgttct cgatttgccg gacacgctcg cggctgatct gcaactgctc
     5641 tcccacctcg gccagcgtgt gctggtgtcc ctgtccaagc ccgaatcgaa gctgaagcac
     5701 cagcttctct cgggcggaga gggtatcgag cgcagcactt acgtcgcgct tgagcatcga
     5761 ctcgctggcg gcctcgtacg gcgtctcgga cacctcgtcg gcgatcaggt cgccgaggct
     5821 tgtgtcgtcc tcgttgccga tcggggtctc cagcgagatc gtccgctgcg ccgcctggac
     5881 gatctggtgg atcttctcgg gcgggacgga catcgcctct gcgacctcct cgggcgttgg
     5941 ctggcgtccg aggtcctgcg cgagcaggtt cagcgtcttg cggtacttcg agatcgagtc
     6001 gcccatatgg accgggagcc ggatcgtgcg gctctggtcc gcgatggccc gcgtgatggc
     6061 ctgccggatc caccaagtcg cgtaggtgct gaaccggaag cccttgcgcc agtcgtactt
     6121 ctgcacggct cgcatcaggc cgatattccc ctcctggatc aggtccaaca gagtcaggcc
     6181 acggttgacg tatcgcttcg caatcgagac cacaagccgc aggttcgcgc ggacgaactg
     6241 ctgcacggct gtgaggtcgc cgttctccac gcgcttggcg aggtcgatct cctggtcatg
     6301 ggtcaggagg ggcgtctcgg caatctcgcg gaggtagagc cgcactggat cattgatgaa
     6361 gttgggatcc agtttttgaa ggacatcgag gtcgcgctca agatcagctt cctgctcgac
     6421 ctcgtcctca ccagcgcgca tctgggtctg gatttcttcg ggaacttcga ccttgtcgtc
     6481 ggagttcatg ttgtccttga ccagatcgtc gatcttggca acgccatagt tgcccatctc
     6541 gtcgttcata tgggggtcaa actccttctg tcactcgaca tggtgcagcg cgcaagaacg
     6601 gttccacgct ggtggggtcc ggcaagctgc tgcccagtca gttttcgatg cttggaattc
     6661 gagtgtgtcg cacgaggtgc accccggcgt atctgcagtt gacacgagca gacccatccc
     6721 catgaacgga gcatgtgtca gcggggatga caagttgctc ttcgtgcggg atagcggctg
     6781 gtgcgatccg ccggaactaa cgaactcggg cgaacacact ggtccgcctc tagccgctac
     6841 agaatgccta gtataggaaa ctgttgacgt tttgtaaacc cgggttgtgt aatacgcgca
     6901 caacagagca cacgtccaca cctgctggcc tgcactcccg ctgaagcgcg tgccgctggt
     6961 gtgaccgtgc gccggtttcg cgtgtgacgc tcgctagacg cggcccgcgg acgctacgcc
     7021 tgtgccgcag ccctgcgcgg tgcccgcgtt gccgccagcg tcgaacgagt ggccacaggc
     7081 acagcccgct accgcgttcg ggttgctgac agtaaaaccg ccgcccatca gcgagttgac
     7141 gtagtcgatc tcagcgccct cgaggtacat cgcgctgaag ctgtcgacca gaacgcgcac
     7201 gccctcggtc tcgaccactt catcgccctc atccggctgc tcgtcgatgg tcatgccgta
     7261 ctgcagccct gagcaaccac ccggtgcgac gaagacgcgc agtgcgtggt cgggagtgcc
     7321 ctgctcgtcg aggaattcgc ggagctgcga gacagcctcg gacgtcatgg tgatcatgcg
     7381 tatcatttcc tccatgatgg agaggtcatt tcgaattggc gcggtagtat gtacctaccg
     7441 cttcccttcc gtaagcatag cggcagggcg ggtgatgtca aggcgccgat cgttttggga
     7501 accggggttt tgtggcgcct gtacatggat gcaatgcgtc tcgtcggcca tcgggtgctg
     7561 aacacgcgcg ggagtgacgt ttgggaaaac gacgacggcg aggcggtggt caagaccggc
     7621 aggatcaggc tgcggcggcg atcgagcagc tcgtggagcg cgaggcgcct ccagaggcca
     7681 tgcggggtgt ggtcgtgcgc gcacacggtc tctggtatga agtgcgaact cacgacccgg
     7741 aagcgacctg gatcgcgacg atgcgcggcg tgctcaagaa gaaagcgcgt cgtaccgata
     7801 tcgttgccgt cggtgatcac gtctggatca ccgatgctgg tgatggtgag gcggtgatcg
     7861 aacgggtcga gtcccggaag tcggttttcg cgcgcaccgc ccgcaacact cgggacaccg
     7921 agcaggtgat cctcgccaac ccggatcagg tgctgatcgt tttcgcggcg cgcgagccgg
     7981 agccgcacct gcggatgctg gaccgattca tcatcctggc ggagcgccat gacctgtcgg
     8041 tgcggatcgt ggtcaccaag accgacctcg agcagaatct cggatccaat gacccgctcg
     8101 ggcccgccga gaagcgtttt gcgctctacg agtcgatcta cccggttcac tacgtgagct
     8161 cggtcaccgg cgaagggctc gaggaacttc gcgaggagct cttcggtcac atctcggtgg
     8221 tggcagggcc gtccggtgtg ggcaagtcca ccctgctcaa cgccctcgat cccaggcacg
     8281 agcgggagac gggcgaggtc tcggaggcaa ctggcaaggg gcgacacacg acgatcggcg
     8341 cgcagctcta cgagatcggg cccgatacct acatcgccga tacacccggg atgcgggcgc
     8401 tggcgatgca tgcggtgccg gaggaggagt tggagtggtg ctatcgggag ttccgggagt
     8461 acctcggcga gtgccggttc agcggctgca cccacatgca cgagcctggt tgcgcgatcc
     8521 gggaggcagt ggagcgcggc gagatcccgg aggaccgcta cgagagctac gtgatggtcc
     8581 ggcaggagag cgaagggtag cggaccccgc gcagccacct gacctgtgcc gtgtccttca
     8641 ggtgctccgg tcgtcgagat gacacggtgg ctgacagcgc gggaggtcgt tgcgtggctc
     8701 gatccgagac tcctactagt agatgaggag cgtgttcatg caggggcaca tcggcaaggg
     8761 cggcgtaacc gtcgaggaca ttgcccgcga catggaggac gtcatcgacg atctgctcca
     8821 gcggatcgag ggcgaggcct tcaccacaac ccagttcatt gaactgttgc gatcggtgcc
     8881 tgagggggag gcagcctacg aggcggcctg gcgccgttgg ggcgagcagg agcacgagag
     8941 caagatggtc atccatggcc aggtcatccc gctcgtgctg cgccggtcgc gctttgccag
     9001 atgggatggg tacgctcacg gcgaggatga tgcgtatgcc gttcctgcct ggtgggtgct
     9061 ggtgtcctcc ggtgacgcac cgaaggacac gggccttcgg tagcgcagag gacgctcacg
     9121 tcgtggcgtg tgtccatggg gtgcgatacc ggggaccgcg gatgtgtaga attgccaaag
     9181 gagcgccgat tcgctcatgc aatcagcgag ccagccgaca cgcaggcttc ggccccgtcc
     9241 ggggtcaccg gaagatgccg gtgcacggat gccccaggaa agacacacgc atgccacaaa
     9301 aggaccaaga aacgtcgcag ttctcgatga ggcgaccgga tcaggacggt aatcggggtc
     9361 gccgtcccca gcccggcaac gacggccgtc ccgacccgga catgcccgag cggcgctcgc
     9421 ccaggattcc gacctgggtt gtcgcgaccc tgatcatcgc tcttgtaggg tggtacatct
     9481 ggcagttctt cgggcctcag gacgagggcg acgcgctgga cgtcccgtac acgacggtga
     9541 ccgagcagct cgtggagggc aacatcgagc gggcggtgct cagcgagacg agcattgcag
     9601 cacaactcga gtcaccgatc ctctggaaca ccgacaacga gcaggtcgac cccgatgccg
     9661 tggccgatgc ccccggcatc gtggaaacga ccgagatcga ggccaccctc ccacagggcg
     9721 tcgaaatccc tgacctgctg cccgcgctgc aggaggccga tgtcgtcatc gagggcgagg
     9781 ccgccggcgg gacaatatgg acgagtatat tgatctcgtt cttcccgttc ctgctcatcc
     9841 tcggcttgat cctcttcatg ggtcgccaga tgagccgtgg ccagcagaac gtcttcgggt
     9901 ttggccgcag caaggcgcgc cagaacgatc ccgagcggcc acaggtgacc ttcgatgacg
     9961 tcgccggcga ggacgaggcc aagcgggagc tcatggaggt cgtcgacttc ctcagtaatc
    10021 cggccaagta tcatgcgctc ggggctcggc tgccccgcgg tgtgctgctg gtcggcccgc
    10081 cgggtaccgg caagaccctg acggctcgtg cggtggccgg tgaggccggc gtgccgttct
    10141 tcagtgtctc ggcgtcggag ttcgtcgaga tgttcgtcgg tgtcggcgcg agccgcgttc
    10201 gtgacctctt cgacaaggcc aaagcggcct ccccagccat tatcttcgtc gacgagctcg
    10261 acgcagtggg tcgccagcgg ttcgctggtc tcggcggttc caatgacgag cgcgagcaga
    10321 cgctcaacca gctgctcgtc gagatggacg gcttcgagac caaccaggaa gtcatcgtga
    10381 tggccgccac caaccggcca gacgtgctcg atcctgcgct gctgcgcccg ggccgcttcg
    10441 accgacaagt gaccgtcgga ctgcccgatc gcgagggtcg cctagcgatc ctcaagatcc
    10501 acacgcgggg catcccgctg agcgcgtcca tcgacctcga gcagatcgcc aggtcgacga
    10561 ccgggttctc cggcgcggat ctctccaatc tcgtcaacga ggcggcgctt actgctgccc
    10621 ggcgcaattc gaaggacatc cgcaaggagg actttgacga ggcgctcgac aagatcctcc
    10681 ttggcgtcgc aaagtccggc ctgaccaacc cgaaggagcg tgaggtggtg gcctaccacg
    10741 aggcggggca tgcgctggtg gcgcacttca cgcccggctc cgatccgctt cgcaaggtga
    10801 gcatcgtgcc gcgcggccgt gcgctcggcg tgaccgtgca gatgcctgac gaggatcggc
    10861 acaactactc gcgccagtac ttgctgggga ggctcgcgat gatgctcggc gggcgagcgg
    10921 cggagatggt ggtctttgac gaaatcacga ccggtgcgga gaacgacctc aagcaggcga
    10981 cgagtctcgc tcgccgcatg gttggtctct ggggcatgag cgatgccgtc ggtccgctct
    11041 acctcggcac cggcgaggag cacgtcttcc tcggtcgcga gatcacgcag gacaagtcct
    11101 actccgacgc aacggccgag aaggtcgacg ctgcggtgcg agggatcgtc gagaacgcgc
    11161 tgcgcgaggc cgtcgacctc acctcgcagt accgtgagca gctcgatggg atggttcggg
    11221 cgctcctgga aaaggaaacg ctcgacgcgc cggaggttat cgagatcttt ggtcctgccg
    11281 tcgacgagga cccgaacgtc ggtatcgacg atgatgaggg actgtctgca aggcaaccgc
    11341 gtgctgaaga tgggcgtcag acggccggta ctgaggacgg gacgatcgtt cgtcgcaagg
    11401 ttggtgactc cggcgcttcc tgacacgcag ccatggcccg tcgaacgaaa cgaccgcccg
    11461 gctcgctgcg agccgggcg
//
LOCUS       DASYWL010000130         6587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_419251, whole genome shotgun sequence.
ACCESSION   DASYWL010000130 DASYWL010000000
VERSION     DASYWL010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6587
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419251"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..1920
                     /locus_tag="VGR22_04605"
     CDS             148..1920
                     /locus_tag="VGR22_04605"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEV2127882.1"
                     /translation="MYNLADTHPTPQFARPRWHDLTGEWQFAFDPEDVGRDAHWESHS
                     AAFDRIIIVPFPPESTASGIGDPNPHRVVWYRRVVDLDVLPREHGERLLLRFGAVDYR
                     ADVWVNGAHVAFNEGGHTPFHADITQPLANQDVADIVVRAEDNPHDLGQPRGKQDWQT
                     PSHGIWYRRTTGIWQPVWLEATGRHRISDLHWQPDADSLTVDAATSITPASSLPLTLH
                     VELALNGDTLADDRYTFSGDRHTARWQLGAPGNPETRRTLIWSQEHPNLIEAKLSLLD
                     GEQVLDRIYSYTGLRSVAVDANGLVLNRFPVYLRMVLEQGFWPESHLAAPSAEAIRRE
                     VELIRDLGFNAARIHQKIEDPRFLSWCDRLGVLVWGEMANAFTWNETAVTRMTREWTD
                     AVLRDRNHPSVVAWVPINESWGVPALPVDARQRHWQEAHWHLTHALDGTRPVISNDGW
                     EHTTSDILTIHDYTESGDEIREKYGADGIGTMLERNRPNRRVLQLEPGANVGRPVMIT
                     EYGGISYAPDAGERWYGYGTVTSEADYIDKVRELTEAVRACPDVTGFCYTQLTDTEQE
                     TNGLLTADREPKLPVESLREIFGQ"
     gene            complement(2249..2503)
                     /locus_tag="VGR22_04610"
     CDS             complement(2249..2503)
                     /locus_tag="VGR22_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127883.1"
                     /translation="MAKCTATSKRSEQRCNNAPVTGAATCRMHGGTAAIANRGRGNGN
                     YKHGRHTKDFGESRLKQRYLAARTDPELRPSEPRTPSAMF"
     gene            3087..3512
                     /locus_tag="VGR22_04615"
     CDS             3087..3512
                     /locus_tag="VGR22_04615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEV2127884.1"
                     /translation="MAFSDTLARINRRVFNPLIRTFAGWLPPLAVVLHRGRVSGRVYR
                     TPVLAFRSGDRVVIVLFYGADTDWVRNVLARGGCELRTRGRTIALTTPEVQPADARPS
                     AVPVPVWVAVRILGIQSFLHGKIHAKGESTESDGDRRNH"
     gene            3717..4364
                     /locus_tag="VGR22_04620"
     CDS             3717..4364
                     /locus_tag="VGR22_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127885.1"
                     /translation="MLLTTLLAMLLGVVQTPQAADDDPNLFLDLGSEHCATEIAFAAF
                     ERTDEDAAVTNVLPAYVTYSAFQFETAEQAEAALEDAPVLVAETFSDDPDIGDTETFD
                     QIANEVQTEDYGDRTLGYAMTLPVDNEALDNALTVELLGIVKHDQLVLILLFSEAGPR
                     RLSPTLTLDTVPPFAEDLDEDWDGTGDPEEAIPDEEGVPIGWERREVTIDDPPSC"
     gene            5037..5501
                     /locus_tag="VGR22_04625"
     CDS             5037..5501
                     /locus_tag="VGR22_04625"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEV2127886.1"
                     /translation="MASFTLRVNGTEHSVDVDENTPLLWVLRNDLGLTGTKFGCGLAQ
                     CGACTVHLDGASTFSCVTPVGEVGDQEVTTIEGLAADDGLHPLQEAWIEAQAPQCGYC
                     QSGQIMSAAALLEANPSPSDDEITEAMSDNLCRCGTYNRIRQAIKMAAGSQS"
     gene            5516..>6587
                     /locus_tag="VGR22_04630"
     CDS             5516..>6587
                     /locus_tag="VGR22_04630"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEV2127887.1"
                     /translation="MTSQRTTPTISRRRLVQSSVAAGGLTIFWSYGASAHQEATPANA
                     TPIATPEAGATPAATPEAETTRLEPPTNVDAYLRIDEDGTITLLTGKVEFGQGIRTGF
                     AQLVAEELSVPFEAVDVIMGKTDEAPFDIGTFGSLSTRLTGPRIRQAAAAMRQWLIDF
                     AAEEHGRSSSGFVLENGKVIATDHSGIEIPYAELAAGKSIARELDPDIRLTSPENFTV
                     IGQSIPRPDVADKVDGSAIYGIDVVVDDMLWAQIVRPPAFGATLVEVGFSEAEGMPGV
                     VSTFRDGDFAGLAAETLQQAQAAIGAVNATWEEPDTDVTHETIFDYLIETADEGQKLG
                     DDNTSPGASDDLTSMILEPLKVT"
BASE COUNT         1201 a         2216 c         2041 g         1129 t
ORIGIN      
        1 gatgcttctc gacgtgctgg tcgcgacgga agaccggtca cagccgcggg aagcgcactg
       61 gtaggcaggg tgccgccctc tatcacgcca cagtcgccct agaatgaccc gggcgttgga
      121 cgaactgaat cgactggaag ctcacacatg tacaacctcg ccgatactca cccgacaccc
      181 cagttcgctc gtccgcggtg gcacgatctc acgggcgagt ggcagttcgc cttcgacccc
      241 gaggacgtcg gtcgcgatgc tcactgggag tctcattctg ccgcgtttga ccgcatcatc
      301 atcgtgccgt tcccgccgga atcgaccgct agcggtattg gtgaccctaa tccgcaccga
      361 gtggtgtggt accggcgcgt ggttgacctc gacgtcctgc caagagagca cggtgagcgc
      421 ctgctgcttc gcttcggtgc cgtcgattac cgcgccgacg tctgggtgaa cggtgcccat
      481 gtcgccttta acgagggtgg ccacacgcct ttccacgccg acatcacgca gccgctagca
      541 aatcaagacg tcgccgacat cgtcgtgcgc gccgaggata atccccacga tctcggtcag
      601 ccccgtggta agcaggactg gcagaccccc tcacacggca tctggtaccg gcgcacgacg
      661 ggcatttggc agccagtctg gctcgaggcc acggggcggc accggatatc agaccttcat
      721 tggcagccag acgcagactc gcttacggtc gatgcggcga ccagcattac accagcgtca
      781 tcgttaccgc tgacactcca tgtcgaactc gccctcaacg gtgacacgct cgccgacgac
      841 cggtacacat tctccgggga tcgccacaca gccagatggc aactcggtgc ccctggcaat
      901 cctgagaccc gccgcacgct gatctggtcg caggagcacc cgaatctgat cgaggccaag
      961 ctgtcgcttc tcgacggtga acaggtcctc gacaggatct acagttacac agggctccgc
     1021 tcggtggccg tagatgcaaa cgggctcgtc ctcaatcgct tccccgtcta tctgcggatg
     1081 gtgctggaac aggggttctg gccggagtcg cacctcgcgg ctccgtccgc cgaggcgatc
     1141 cggcgcgagg tcgaattgat ccgcgacttg ggattcaacg ccgcccgtat ccaccagaag
     1201 atcgaagatc cgcgattcct gagctggtgt gaccggctcg gcgtgctcgt ctggggcgag
     1261 atggctaatg ccttcacctg gaacgaaact gccgtcaccc gcatgacacg agagtggacg
     1321 gatgccgtgc tgcgtgaccg caaccacccc tcggtcgtcg cgtgggtgcc gatcaacgag
     1381 agctggggcg tgcccgcgct gccagttgac gcccgtcagc gccactggca ggaggcacac
     1441 tggcacttga cgcacgcgct cgacggcacc cgcccggtga ttagcaacga tggttgggag
     1501 cataccacca gcgatatcct gaccattcac gattacaccg agagcggcga cgaaatacga
     1561 gagaagtacg gcgctgatgg gattgggacg atgctcgaac gcaaccgccc gaataggcgc
     1621 gtcctccaac tcgaaccggg cgccaacgtg ggtcgtccgg tgatgatcac cgagtatggg
     1681 ggcatcagct acgctcctga cgccggcgag cgctggtatg ggtacggtac tgtgacgagc
     1741 gaagccgact acatcgacaa ggtgcgggag ctgaccgagg cagtccgtgc gtgtcccgat
     1801 gtgacggggt tctgctacac ccagctgacc gacactgagc aggaaaccaa cggcctgctg
     1861 accgccgacc gagaaccgaa gctcccagtt gagtcgctgc gagagatttt cggtcagtaa
     1921 cagaggtcaa cgcaaacaga gctcgtacta tcaatgtgaa tcggtccgat gcgtatatgg
     1981 ttctcgcgcg caagatgaca cgtcccacca gacgtagctg ctcaaggagc cgtcctaccc
     2041 cacgggcatc gatgcccatg cggatccggt cccctctcca tagtgctatc gcaccacgtg
     2101 aactgagctt gagatacacc tcgtgaacta tctcggatag tgccgggaat ttggtgtaag
     2161 gtaccaggac gaggcacaag cagaccgcat gccaccaagg atagatggga atggattatc
     2221 gccagcacgg gcaggtcgtc agcttcatct agaacatcgc tgacggcgtc cgcggctcgc
     2281 tcggccgcag ctcgggatcg gtccgcgccg ccaggtagcg ctgcttgagc ctggactccc
     2341 cgaagtcctt ggtgtggcgc ccgtgcttgt agttgccgtt gccccggccg cggttggcga
     2401 tcgccgccgt gccgccgtgc atgcggcagg tggccgcgcc ggtgaccgga gcgttgttgc
     2461 accgctgctc gcttcgcttg cttgttgccg tgcacttggc catggtgccc ttccatgcaa
     2521 gggttgtctg ttcgtgcaag gggtgattgc tcgtacgctc gctcgccggc tgcggagtgt
     2581 gctggccccg aacccagcgg cgcgctggaa cgacccaacg gcgtcgtcac cctccccacc
     2641 caagtctagg gcagccagat gggcgaacgg gccacctacg gggaccatcg gtacggcgca
     2701 gtgcttgccg tgggagacag cttgacgacg agggaggcga cctgcgcaac gccagcgagg
     2761 ccagtaaatg cgattcgcgc cttctcgata ggtccacgtt taattcaagg gagatcggct
     2821 tcagcaacat gattgggggc caccgatgtt cgacacctcg ccgctgccac ccgctcaacg
     2881 gcgattcgcc gcgccccttc ctgccggtca cgggcgacaa aacctggtcg cccatgtgcc
     2941 agacgctgcg cctcacgaga cgcatgtacc gtccgctcga cctgggcggt gaccgatatt
     3001 catatccgtc taccatgtcg tcttgcgtgc attaggcgca agccggcaag gtgcgaacgt
     3061 cacaaccgca gcgtgtggag ggcaccatgg ccttcagcga tactctggcc cgcatcaatc
     3121 ggcgcgtgtt caacccgctc atccgcacct tcgccgggtg gctgccaccg ctcgcggtgg
     3181 tgctccaccg cggccgtgtc tccggacggg tataccgcac gccggtgctg gccttccggt
     3241 ccggtgaccg cgtcgtcatc gtgctgttct acggagcgga taccgactgg gtcaggaacg
     3301 tcctcgcccg gggtggctgc gagttgcgca cccgtgggcg aacgatcgcc ctgacgaccc
     3361 ccgaggtgca gccggccgac gcgaggccca gcgccgtgcc tgtacccgtg tgggtggcag
     3421 tgcgaatact cggcatccaa tcgttcctcc acgggaagat tcatgccaag ggtgagagca
     3481 ccgagtccga tggggaccga cggaaccact gacatcccga tgacccgacc ctcaccccgg
     3541 aactgcgatc cctctgcgcc accgtggcct gcgaggcgca ctccggagct gcctagcaga
     3601 attccccatc gcgctctgga gccatgccac aaaaacttga tagctggcgt gaaagttgcg
     3661 agattcgcta ccagaaccag atgctgtcgg tcgctcgttt ctctggagtg cccaccatgc
     3721 tcctcactac cctgctcgcc atgctgctcg gtgtcgtgca gacacctcag gccgccgatg
     3781 acgaccccaa cctcttcctc gacctgggca gtgagcactg cgccaccgag atcgccttcg
     3841 ccgccttcga gcgaaccgac gaggacgcgg ctgtcaccaa cgtcctgccc gcctacgtga
     3901 cctactcggc attccagttc gagaccgcgg agcaggctga ggccgcgctg gaggacgccc
     3961 cggtgctcgt tgccgagacg ttcagcgacg accccgacat cggtgacacc gagacgttcg
     4021 accagatcgc gaacgaggtg cagaccgagg actacgggga tcggacgctc ggctatgcga
     4081 tgacattgcc cgtggacaac gaggcgctcg acaatgcgct gacggtcgag ctgctgggca
     4141 ttgtcaagca cgatcagctc gtgctcatcc tcctgttcag cgaggccggt cccagacgct
     4201 tgagtcccac cctcaccctc gacacggtcc cgccgtttgc cgaggacttg gacgaggact
     4261 gggacggcac cggggacccg gaggaagcca tccccgatga ggaaggcgtg ccgatcggct
     4321 gggagcgccg ggaggtcacc atcgacgatc ctcccagctg ctagacgtcg cggtatgcct
     4381 tgatccccag tcggcgtgcg ggcgttgccg gaaccggccg tccctatcct cgcaggctat
     4441 gttgcccaac cccagagacg tgccgtgacc ccatgcacac ctgtcgcctc gtgcgcgcct
     4501 gcgcaaggtc tcacgccggc ccgatcgcct acccgccgcg cgctagccgc cgcaacgtgc
     4561 cccagaggcc ctcagcacgg ccttggggcg tctctgggcc ggcgttggta tggtcccggg
     4621 tctgacctct cgccgggttt atcgccgcct gcgggcaatg caagcgcctg ggcacgctct
     4681 gcaaggcgct gggccgtctc tgccataacc cgcgagcgat gggcatgctc tcgccagtgg
     4741 tcacgattgg cggaaacctc ctcgacgatc cgctcgaggc cgaccatctc tgcccgcaag
     4801 cactcaagct cgagcgccgc gtgggaagta atccattgcg gtgccgcggt tcccggatct
     4861 cgggctcaac gccagatacg accctgctgc atatggttgc gtccccggct tcccctcccc
     4921 tgcttccacg ccgatggccc cgtacttgcg atggtgtcgc aacctatcac aatatcgccc
     4981 gggatctggg cattcgcggg agtgcaccac gtccgccggg caaaggggct tcactcatgg
     5041 ccagtttcac gctcagggtc aacgggacgg agcattccgt cgacgttgac gagaacaccc
     5101 cgctgctgtg ggtcctgcgc aatgacctcg gcctaaccgg aaccaagttc ggctgcggcc
     5161 tcgcgcagtg cggcgcctgc acggtacacc tggatggtgc ttcgacgttc tcgtgtgtca
     5221 ccccggtcgg tgaggtcggg gatcaggagg tcaccaccat cgaggggctc gccgccgatg
     5281 atgggcttca tccgctgcag gaggcctgga tcgaggcgca ggcaccgcag tgcggctact
     5341 gccaatccgg gcagatcatg tcggcggctg ctctgctgga ggccaacccg tccccgtccg
     5401 acgacgagat caccgaggcg atgagcgaca acctctgccg gtgcggcacc tacaaccgca
     5461 tccgccaagc catcaagatg gctgcgggca gccagagcta agggagcatc atgccatgac
     5521 atcgcagaga accaccccca cgatttcccg acggcgtctc gtgcagtcga gtgttgccgc
     5581 cggcgggctg acgatcttct ggtcgtatgg cgccagcgct caccaagagg ccacaccggc
     5641 gaacgcgacc ccgatcgcga caccagaagc tggtgcgaca ccagccgcca ccccagaagc
     5701 ggaaaccacg cggctagaac cgcccaccaa cgtggatgcc tacctgcgca tcgacgagga
     5761 cggcaccatc acgctgctga ccggcaaggt cgagttcggg cagggcatcc ggaccggctt
     5821 cgcccagctc gtcgccgagg agctctcggt gcccttcgag gcggtcgacg tcatcatggg
     5881 aaagaccgat gaggcgccgt tcgatatcgg cacgttcggc tcgctcagca cacgcctgac
     5941 cggtccgcgc atccgccaag ccgctgccgc catgcggcag tggttgatcg atttcgccgc
     6001 cgaggagcat gggcgttcca gcagtggttt cgtgctcgag aacggcaagg tcatcgccac
     6061 cgaccacagc ggcatcgaga tcccgtatgc ggagctcgct gccgggaaga gcatcgcccg
     6121 cgaactcgac cccgacatcc ggctcacgtc accggagaac ttcaccgtca tcggacagtc
     6181 gatcccacga cccgacgtcg cagacaaggt cgatggctcg gcgatctacg gcattgacgt
     6241 ggttgtcgac gacatgctgt gggcccagat cgtgcgcccc cccgccttcg gcgcgacgct
     6301 cgtcgaggtc ggcttctctg aggcggaggg gatgcccggg gtcgtcagca ccttccggga
     6361 cggtgacttc gccggcctcg cggccgagac gctgcagcaa gcgcaagcgg ccatcggagc
     6421 agtgaatgcc acgtgggagg agcccgacac cgacgtcacg cacgagacca tcttcgacta
     6481 cctgatcgag acggcggacg aggggcagaa gctgggcgac gacaacacca gcccgggcgc
     6541 cagcgacgat ctcacgtcga tgatcctcga gccgctcaag gttacct
//
LOCUS       DASYWL010000131         5199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_625932, whole genome shotgun sequence.
ACCESSION   DASYWL010000131 DASYWL010000000
VERSION     DASYWL010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625932"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VGR22_04635"
     CDS             complement(<1..352)
                     /locus_tag="VGR22_04635"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEV2127888.1"
                     /translation="MTDTPHPLISAHRGGREEAPENTLAAFQRARDLGVPGAECDVRL
                     SVDGVPVVIHDDTVDRTTDGTGAVHALTVAQLAQLDARAVHTNWPTTPHVPTFAEFMA
                     VAEGFAYIEVEIKAD"
     gene            564..1358
                     /locus_tag="VGR22_04640"
     CDS             564..1358
                     /locus_tag="VGR22_04640"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEV2127889.1"
                     /translation="MDVDIDWGTADLTAMHGLSYIDLLLRLGVATLLGALVGLERERL
                     ERAAGFRTHALVCVASALIMVVSIFGFPQSDVLDPSRIAAQVVSGIGFLGAGVIIFRR
                     NTVRGLTTAASLWSVAGVGLAVGAGLYIPATAGTVFMLLIQAGMRPIEAHFFKHESRR
                     QRLLIETTDASGLLKGVPIIGARHGMRVQSMQFERDSSTDTDVVELTIRLENLNERFG
                     IVSDLQEVPGVVRVHDGFGPAVLRRTGRRGIGFQEDDEDEVNGGIV"
     gene            1566..1847
                     /locus_tag="VGR22_04645"
     CDS             1566..1847
                     /locus_tag="VGR22_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127890.1"
                     /translation="MSEQFKAALVRALIVGGLGALATFLATWATTDDGTVIIVAVAAA
                     FVASFLARFGGEGAWDTKRAKDGDVQPADVGYDELHALRTGRVRIVTET"
     gene            complement(1979..3349)
                     /locus_tag="VGR22_04650"
     CDS             complement(1979..3349)
                     /locus_tag="VGR22_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127891.1"
                     /translation="MRVDTEPPETPRLGDRSPSRRGRQEEPASIPSALIVPTTVFSPV
                     ELQGARERLTSGDGLHRLLASIGGDEASDSSLPLPARLAAPGFFRRHRASVATVFLYL
                     ALVVIPGFLISAFGDRFTSREFVRLTDWREFSGALFLYLVLAPVLWTFYLWQPRLIVE
                     VFAGLAQSGAIGPARRADIAADAVLRRMGGSFVETAGRIRAMRVTKGALLAALSLAAS
                     VATLLVWPPTALPPVNRLFPDSDLFWWQIVPVYFWAVWLPLVFVNVYMLVWIVIRQTV
                     MIANIQRLLSLFEVEPIPFHPDGSCGFAPIGSYATNIVRIALIIGAWALVLLLSGPVT
                     GHGVYVAPHILFLVIVQVLLTPYLLFGPVWYAHRVMRAARERALQRVGDAIRTSLLGD
                     VRPSAARMAREDRYQELEAQYRLVEEGYETWPFGRTAFSGVSITAGVTLAGNVAAILY
                     RMYIGT"
     gene            3508..3993
                     /locus_tag="VGR22_04655"
     CDS             3508..3993
                     /locus_tag="VGR22_04655"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2127892.1"
                     /translation="MAKPSNGGKRILVVNDDPAILALFEELLGEEGYEVTIDRFARQT
                     GDLLQSIRDASPDLVIMDFIIGREESGWQLLQAMRMDRTTRDIPIIMCTGAVKQVTEL
                     REQLDTMGVQVVTKPFDIDHLLEVIAAVWAGSPTPRSALRDESAGRDANQAKGDSAVM
                     E"
     gene            complement(4053..4244)
                     /locus_tag="VGR22_04660"
     CDS             complement(4053..4244)
                     /locus_tag="VGR22_04660"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HEV2127893.1"
                     /translation="MANPVDPVCGMELTPGQVEAQARYKGKAYYFCSEECRRTFEENP
                     EAYVSTSADQGDAPTTTPR"
     gene            complement(4309..4575)
                     /locus_tag="VGR22_04665"
     CDS             complement(4309..4575)
                     /locus_tag="VGR22_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127894.1"
                     /translation="MAHQQRTEGEGREPEHTHPAVTHTHDHWHVSHHVTDAGEVEHRV
                     FWHTHEHNHGEITHSHDYGREEEERDHAKEAHIHDHAAPTESPA"
     gene            complement(4619..4948)
                     /locus_tag="VGR22_04670"
     CDS             complement(4619..4948)
                     /locus_tag="VGR22_04670"
                     /inference="COORDINATES: protein motif:HMM:NF023174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HEV2127895.1"
                     /translation="MLFYCEFTWFPGTTREEVARRVVEQHDAGLNHPDRIKGWYNLAG
                     GGAGFLIVDYDDVAELTAFLQPYMEFMSFDVRAIHELDYNSRIEELRKAASQAGTASG
                     VARHDFA"
BASE COUNT          934 a         1525 c         1786 g          954 t
ORIGIN      
        1 catcggcctt gatctcgacc tcgatgtagg cgaagccctc ggcgaccgcc atgaattcgg
       61 cgaaggtcgg cacatgtggc gttgtcggcc agttggtatg cacggcccgg gcgtcgagct
      121 gggcgagctg ggcgacggtg agggcgtgca ctgcgccggt gccgtcggtg gtgcggtcga
      181 ctgtgtcatc gtggatgacg acgggcacgc cgtcgacgct gaggcggaca tcgcactctg
      241 cgccggggac gccgagatcg cgtgcgcgct ggaaggcggc aagggtgttt tccggcgctt
      301 cctcccggcc gccacgatgg gccgagatga gggggtgtgg cgtgtctgtc atgtcttcac
      361 gcttcccgga atgtgtaatg gtggcgtgtg ccgcgctgcc acggctgccg cccgcatcct
      421 agcatcaata tccatgccgg gccacgaccg catgttccct gaggccagtg ggtgatcccg
      481 ccggagtgtg gcggccggtc gggtactatt gcgtggatga tcaatgtggc gtcaacagat
      541 tggcccggaa ggatttcgtg taggtggatg tcgatatcga ttggggaact gccgatctga
      601 cggcgatgca cggcctcagc tatatcgatc tgctgttgcg gctcggtgtg gcgacgctgc
      661 tcggggcgct ggtcggcctc gaacgcgaac gcctggagcg ggccgctggg ttccggacgc
      721 atgcgctggt gtgcgtggcg tcggcactga tcatggtggt ctcgatcttc gggtttcccc
      781 aatcggacgt gctcgatccc agccgtatcg cggcgcaggt ggtgagcggc atcggcttcc
      841 tcggtgccgg cgtgatcatt tttcgccgga acaccgtgcg cgggctgacc acagcagcga
      901 gcttgtggtc ggtagctggc gtcgggctcg cggtcggcgc aggactctac atcccggcga
      961 cggccggtac cgtcttcatg ctgctcatcc aggccggcat gcggccgatc gaggcgcact
     1021 tcttcaagca cgagagcagg cggcagcggc tcctgatcga gacgaccgat gccagtggcc
     1081 tgctgaaggg cgtaccgatc attggggcac ggcacgggat gcgggtgcag tcgatgcagt
     1141 tcgagcgcga cagctcgacc gacaccgacg tggtcgaact gacgatccgg ctggagaacc
     1201 tgaacgagcg tttcggcatc gtcagcgatc tgcaggaggt gccgggggtg gtacgcgtgc
     1261 acgacggatt cggcccggcg gtgctgcggc ggaccggacg gcgcgggatt ggctttcagg
     1321 aggatgatga agatgaggtg aacgggggta ttgtctaaga tcgtgcgtca ataggtgtgt
     1381 atgctgtggc agcattccgg gcaacaaccc cattttccgt agctgcggct caccaggcgc
     1441 agccaggagg tcaacctctt gctggagatt ctcaattatc cctcttccca gtcttgccgc
     1501 tgctgtgaca caatggtgtg atcaggcagg gtttcaagtt cacaacagaa gagggggaag
     1561 acgacatgtc cgagcagttc aaggccgcgc tggttcgcgc gctcattgtc ggtggcctcg
     1621 gtgccctcgc gaccttcctc gcgacgtggg ctaccacgga cgacggcacg gtgatcattg
     1681 tcgccgtcgc ggcggcgttc gtggcgtcgt ttctcgcccg cttcggggga gagggtgcct
     1741 gggacaccaa gcgagccaag gacggcgatg tgcaacctgc tgacgtcgga tacgacgagt
     1801 tgcacgccct gcgaacgggt cgcgtgcgca tcgtgaccga gacgtgagcg cgtgtgctga
     1861 ccgcgtaggt ctgtatgcgt cttgagaggc gtggcactct accctattgg cgcacgctcc
     1921 aagggccagt gctgactcgt tcacaggatg gacgccatgc ggtcgggcgt gcccattttt
     1981 acgtgccgat atacatcctg tagagaatgg cggcgacgtt gccggccagg gtcacgccag
     2041 cggtgatgct caccccgctg aaagccgtgc gtccgaacgg ccacgtttca tagccttctt
     2101 ccaccaggcg atactgtgcc tcgagctctt gatagcgatc ttcacgagcc attctcgctg
     2161 cggacggtcg cacgtccccg agaagagacg tccgaatggc atcccccacc cgttggagcg
     2221 cccgttcccg ggctgctctc atcacgcggt gggcatacca gacgggccca aacaggagat
     2281 agggagtcag gagtacctgg acgatcacca ggaagaggat atggggcgcc acgtagaccc
     2341 cgtgcccggt gaccggaccg ctgaggagca ggacgagtgc ccacgcgccg atgatcagcg
     2401 cgatgcgaac gatgttggtc gcgtagctgc caatgggagc aaagccgcat gagccatccg
     2461 ggtgaaacgg gatcggctcg acctcgaaga ggctgagcag gcgctggata ttggcgatca
     2521 tgacggtctg cctgatgacg atccagacca gcatgtagac gttgacgaag acgaggggca
     2581 gccacacggc ccagaaatag accggcacga tctgccacca gaaaaggtcg gagtcgggga
     2641 agagccgatt cactgggggc agggcggtag ggggccagac cagcagggtt gccactgacg
     2701 ctgccagcga cagggcagcg agcaacgctc ccttcgtcac tcgcatcgcc ctgattcgcc
     2761 cggcggtctc gacaaagcta ccgcccatgc gccggaggac cgcatcggcc gcgatgtcgg
     2821 cgcggcgtgc cgggccgata gcgccgctct gcgcgaggcc ggcaaagacc tcgacgatca
     2881 ggcgtggctg ccacaggtag aaggtccaca gcacaggcgc gaggacaaga tagagaaaga
     2941 gggcaccgga gaactcgcgc caatccgtga gcctgacgaa ctcgcggctg gtgaatcggt
     3001 ccccgaacgc ggatatcagg aaccccggga tcacgacgag cgcgaggtac aggaacaccg
     3061 tcgccacgct ggcgcgatgc cgtcggaaga agcccggtgc ggcaagtcga gcgggcaagg
     3121 ggagactgct gtcgctggcc tcgtcacctc cgatactggc gaggagtcgg tggaggccat
     3181 caccggaggt gagccgctcg cgtgctccct gaagctcgac cggcgaaaag acggtcgtgg
     3241 gcactatcag cgcgctcgga atggaggccg gttcctcctg tcggccgcga cggctgggcg
     3301 agcggtctcc aagacgcggc gtttcgggag gttccgtatc gacacgcatg atgacctcgt
     3361 cgcggatgga agcaatcacc ggatagcatg gccgttaaca ctgtcacagt accatgggaa
     3421 acaccggcag ccacagtggc ggttcgctgc gcggcatgtt gtccttgtcg tcacggtgct
     3481 gttcccacac agtgattgga ggacgtgatg gcgaaaccga gcaatggtgg gaagcgaatc
     3541 ctggtcgtca atgacgatcc ggccatactc gcgctgtttg aggagctgct gggcgaggag
     3601 gggtacgagg tcaccatcga caggttcgcg cgccagacag gcgatctgct gcagagcatc
     3661 agggacgcca gcccggatct ggtgatcatg gatttcatca tcggccggga ggagtccggg
     3721 tggcagctgc tgcaggcgat gcggatggac cggacgacgc gtgacatccc gatcatcatg
     3781 tgtactggcg cagtcaaaca ggtgaccgaa ctccgcgagc agctggacac catgggcgtg
     3841 caggtagtca ccaaaccgtt cgatatcgat cacctgctcg aggtcatcgc agcggtgtgg
     3901 gcgggtagcc caacacctcg gtcggctcta cgagacgagt ctgccggccg cgacgcgaac
     3961 caggccaagg gtgactcggc cgtcatggag taggtatccg cgcagtccgg tgaccgctcc
     4021 cgcaccttgt ggagcggtca ccggttgcac catcagcgag gagtcgttgt aggcgcatct
     4081 ccctgatcgg cagatgtgct gacgtaggcc tcggggttct cttcaaaggt gcgcctgcac
     4141 tcctccgagc agaagtagta cgccttcccc ttgtaccggg cctgcgcttc cacctgtccc
     4201 ggggtcagtt ccatcccaca gacggggtcg acagggtttg ccatgagatc ctccattccg
     4261 tcgatgcgga actctactga accagcgata tgcctctcga gcgacacgtc aggcaggtga
     4321 ctccgttggc gcggcgtggt cgtggatgtg agcctccttg gcgtggtcgc gctcctcctc
     4381 ttcccggccg taatcgtggc tgtgcgtgat ctcgccgtgg ttgtgctcat gggtgtgcca
     4441 gaagacgcga tgctcgacct cgcccgcatc agtgacgtga tgcgagacgt gccagtgatc
     4501 gtgcgtgtgg gtgacggcag gatgggtgtg ttccggttcc cggccctcgc cctcggtgcg
     4561 ttgctggtgg gccatgataa cggctccttt ccactgctat cgcatcgaat gggtcacgct
     4621 aggcgaagtc gtgacgggcg acgcccgagg ccgtgcccgc ctggctcgcc gccttgcgca
     4681 gctcctcgat gcgggagttg tagtcgagct cgtggatcgc gcgcacgtcg aagctcatga
     4741 attccatgta gggctggagg aaggcggtca gctccgcaac gtcgtcatag tcgacgatca
     4801 ggaacccggc accgccaccg gccaggttgt accagccctt gatgcgatcg gggtggttga
     4861 ggccggcgtc gtgctgctca acgacgcgcc gagcgacctc ctcgcgggtc gtgccaggga
     4921 accaggtgaa ctcgcagtag aagagcatgg tgcatacctc caaactgatc gaacgacgct
     4981 gaatgaccag cgaggttttt atggcaagat aggtgccatg gcactcacct ttaggttagg
     5041 aaggcgagcg cccgcagagg tcccatcgat cgtcaatctg ttccggcgcg gaacagagct
     5101 atccaagcgg accgaggatc tcgctcagta ggtcggcaac gtcgtccaga ttggtcttcg
     5161 ggtggtcgag caggtcggcg accgcgcaga gctggttcc
//
LOCUS       DASYWL010000132         7123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_325006, whole genome shotgun sequence.
ACCESSION   DASYWL010000132 DASYWL010000000
VERSION     DASYWL010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7123
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325006"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1278
                     /locus_tag="VGR22_04675"
     CDS             <1..1278
                     /locus_tag="VGR22_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV2127896.1"
                     /translation="LPNFSSEYFNIDADEPWDMGLGVSKARTDKIGAGELYIEHIRRL
                     HEMVSKHGRTMMMWADVFWHHPEKVADLPDDIVLLDWWYEPKERYDTVDTLRGRRFYV
                     CPGTGAWLSLFPRIERAIANVQSFVRDGVEAGAEGMLMTDWGDYGHFTMFENNWYPYL
                     WAAECAWSGAETSVEAYDQAVSHLFFRDLSGNLIAAIRRLGSVIAPVDAERQAITATG
                     PAFWGDPLDRRVERVLPRVQMEQIRQGAEAIFPLLDSVRDPAIRHELGFAAFELVVLA
                     ERRDTFDMLKEALAAADDGGTDREATVATLDTVIDRLEAHRRQIPALAEEFQERWLGH
                     ARHSQIGVSLERFARQVDQYRHAIAWVQEQRDAAARGDAVAGIGAYDRGEFLGIMDEV
                     AVWTNRLADLVGLENLPPDIRSHIESRRQLMEV"
     gene            1532..2626
                     /locus_tag="VGR22_04680"
     CDS             1532..2626
                     /locus_tag="VGR22_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2127897.1"
                     /translation="MAVVSTDATTATTRQPGTVTVWARSRQILTGFLARRILRAILTI
                     FFVTTFIFFLVRLLPGNPIEVYINQQITQYGYSYEDARAQAQSLFAIDVDQPVLLQYF
                     DYLQNLAQGNLGMSVSSPGTTVGEVIESRLPWTLFSVGTALVISFIVGALLGMFMAYE
                     RGRFADSVLSSFASLSGSIPNFLGALLLLIFFGVKLEWIPFVDMRGSLSPGQQVEFSF
                     AFVQDALYHATLPILVYVVFSIGSWMLLMKSSTISTLEEDYVAAGRARGVPEWRIAIF
                     YVGRNAILPLFTQLTIAIGFVAGGSLLIEPIFAYEGIGSELWRSIQQRDYPMLQGIFL
                     VVTVSVVVANLIADLFYSRLDPRIRTGGGE"
     gene            2629..3540
                     /locus_tag="VGR22_04685"
     CDS             2629..3540
                     /locus_tag="VGR22_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2127898.1"
                     /translation="MAEAVVGTGAPSPGVDRAAARQRKSFLRVFIERNKVGMIGYVIF
                     GAILIMSFIGPLFLPDENPTDVGLVYAPPSWDHPLGTDWRGQDVLYQIINGGAGLLIV
                     SMIAAGVATAVAVIFGALAGLIGGRLDSVALILADVVLTVPFIILLGVLAVFVRLDNI
                     FLLALVLAAVGWPTLLRAVRAQVLSLKEREYVEAARMLDLPMRHVLFREVLPNMMPYI
                     IINFVLGMTNAIYGQIILYYLGLVPLQGDNWGLMIQQIQSKQAYSLNDAFVYVMAPIV
                     MISLLQLSLVLISRTLEDVFNPRLREV"
     gene            3544..4653
                     /locus_tag="VGR22_04690"
     CDS             3544..4653
                     /locus_tag="VGR22_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2127899.1"
                     /translation="MATSTQAAQDREAKDRIRGTAESTAPTILEVDDLWVEYKTPAGW
                     MQAVRGANMSIRKGEAVALIGESGSGKSTLGFAILRMLVRGARVSRGSVRFTTSKGET
                     LDLLKMSDREFRRVRWKEIAMTFQAAQSSLNPVMKVKDTFQETARAHGWRNRGEVRER
                     TLDLLQKVQLDPDRVYNAYPHELSGGMRQRVSIALSLLLQPTLIILDEPTTALDILTQ
                     RAIIDVVRNLREELDFTMIFISHDLSLAAELADRVATMYAGEVVEYGPVRDVFYEPQH
                     PYSVGLMNAVPPIAGEEFTPLTAIPGSTPNLLNIPSGCPFHPRCPFHTDICITDHPPL
                     EEVAPSHLAACHHHDEVDKDPERWESIDDVEEAVG"
     gene            4896..5945
                     /locus_tag="VGR22_04695"
     CDS             4896..5945
                     /locus_tag="VGR22_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2127900.1"
                     /translation="MTEPIMQLEHLTRRFKTPKGIIKAVDDVSFTLYPGDILCLVGES
                     GSGKTTAARMAGGLLKPSEGRIIFEGKDIAEMSTEEFAEYRKQVQIIHQDPYASLNPV
                     HTIGDTLRAPLLYHNVVKNRTEATRRAGALLELVDLRPVENFIDKFPHQLSGGQRQRV
                     SIARAMVLNPRVIIADESVSMVDVSIRVSLLNTMLKLRNELGVTFLFITHDLAVVKYF
                     AWEGRIGVMYVGKLIEYGRTPRLIGRPLQPYTRALAAAIPEADPDLTRSKSKLQLRSL
                     DIPNLNDLPDGCVFHPRCPYWVQGLCDVEMPPLVDVGEGRLVACHVVQRNVEAGGDGR
                     DLEATRESMLAHAAD"
     gene            6144..>7123
                     /locus_tag="VGR22_04700"
     CDS             6144..>7123
                     /locus_tag="VGR22_04700"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2127901.1"
                     /translation="MSKDTFEETHRSMLQAPMSRRRAMGVGAGAVGAAMLTGAPLPAG
                     FGRVVMAQDGGAEFHSAYPYQQPPTGHFNTFVTNAILPPMPQGGTVYGELIYEPLGMF
                     YWGTGEWLPLLATEWSFLDDGETFEIKLREGVNWSNDEPFTAADVDATLWCLRIMSNT
                     LWQYVDQVNVVDDYTVQLHMTTPSTVVERYAIRVALPRPASVYGEWADRAKELFEGGL
                     TLDDPEGKQLLDQFNQFRPDPANLPVTGPFTIDPNSITSAQLTMVRNENAWNADQVQF
                     DRIVNFNGETDTITSVVLAGDVDYATHGFAPATESEFLNQGIRVLRPPVYS"
BASE COUNT         1231 a         2307 c         2299 g         1286 t
ORIGIN      
        1 ctgccgaact tctcgtccga gtacttcaat atcgacgccg acgagccgtg ggacatgggc
       61 ctcggtgtct cgaaggcgcg caccgacaag atcggtgccg gcgagctcta catcgagcac
      121 atccggcggc tgcacgagat ggtcagcaag cacggtcgca ccatgatgat gtgggccgat
      181 gtcttctggc atcacccaga gaaggtcgcc gacctgcccg acgacatcgt gctgctcgac
      241 tggtggtacg aaccgaagga gcgctacgac acggtcgaca cgctgcgggg ccggcgcttc
      301 tacgtctgtc ccggcacggg cgcgtggctc tcgctcttcc cccgcatcga gcgggcaatc
      361 gcaaatgttc agagcttcgt gcgagacggg gtcgaggccg gtgccgaggg catgctgatg
      421 accgactggg gcgactacgg gcatttcacg atgttcgaga acaactggta cccctatctc
      481 tgggcggccg agtgtgcctg gagcggcgcg gagaccagtg tcgaagctta cgatcaggca
      541 gtaagccacc tcttcttccg tgacctgagt gggaacctca tcgctgctat ccgcaggctt
      601 ggctcggtta tcgcgccggt ggacgcggag cggcaggcta tcaccgccac cggtccggca
      661 ttctggggcg atccgttgga ccgccgggtg gagcgggtat tgccgcgagt ccagatggag
      721 cagattcggc agggtgccga ggcgatcttt ccgctgctcg acagcgtgcg cgatccggcg
      781 atccggcacg agctcggctt cgccgccttc gagctggtgg tgctggcgga gcgccgtgac
      841 accttcgata tgctcaagga ggcgctggcg gcggcagacg acggcggtac cgaccgggaa
      901 gcgacggtgg cgacgctgga tacggtgatc gatcgcctgg aagcgcaccg gcgccagatc
      961 ccagcattag ccgaggagtt ccaggagcgg tggctcgggc acgcccggca cagccagatc
     1021 ggggtctcgc tggagcggtt cgcccggcag gtcgaccagt accggcacgc gatcgcgtgg
     1081 gtgcaggagc agcgtgatgc cgccgcgcgg ggggacgcag tcgctggcat cggggcctac
     1141 gaccgtgggg agttcctcgg catcatggat gaggttgctg tatggacgaa ccgcctcgcg
     1201 gatctcgtgg ggctggagaa cctgccgccg gatattcgca gccacatcga gtcgaggcgt
     1261 cagctgatgg aggtctgagc cggcaacacg cgccgcatga ggccgatcac gttgcgagtt
     1321 gtgtccctcg ggggtatcct gtccgcatta gctcacgcca gacgatgctg ttgcaccccg
     1381 aggggcacca ctacgcccgt gcaagcggtt ttccaggtgt atctccctcg gtcgcgacac
     1441 gctcgatacc gtgaacggtc gcgtttaccg gtgtatgcca cgcttcgtgc accacaatgc
     1501 gagcaggcat gcgacaggga ctggagtagc catggccgta gtgtccaccg acgccaccac
     1561 cgcaacgacc cgccagcccg ggacggtgac ggtgtgggcg agatcacgtc agattcttac
     1621 cggattcctg gctcgccgca tcctgcgggc catcctcacg atcttcttcg tgacgacctt
     1681 catcttcttc ctggtgcgcc tgctgccggg gaatccgatc gaggtctaca tcaaccagca
     1741 gatcacccag tacggctatt cctatgaaga cgccagggcc caggcgcaat cgctcttcgc
     1801 catcgatgtc gaccagcccg tgctgctgca gtacttcgat tatctgcaga acctggcgca
     1861 gggcaacctc ggcatgtcgg tgtcgtcgcc gggcaccacg gtcggggagg tgatcgagag
     1921 tcgcctgcca tggacgctct tcagcgtcgg gactgcgctg gtcatctcct tcatcgttgg
     1981 ggccctgctc ggcatgttta tggcctacga gcgggggcgg ttcgcggata gcgtgctctc
     2041 ctcgttcgcg tcgctctccg gctcgatccc gaacttcctc ggcgcgctgc tgctgctcat
     2101 cttcttcggc gtcaagctgg agtggatccc gttcgtggac atgcgtggtt cgctctcacc
     2161 gggacagcag gtcgagttca gcttcgcctt cgtccaggac gcgctgtacc atgcaacgct
     2221 gccgattctc gtctacgtgg tcttctccat cggcagctgg atgctgctaa tgaagagcag
     2281 cacgatctcc acgctggagg aggactacgt cgcggcgggt cgcgcccggg gcgtgcccga
     2341 gtggcgcatc gccatcttct acgtcggacg caacgccatc ctgccgctgt tcacgcagct
     2401 gaccatcgcg atcggcttcg ttgccggcgg ctcgctgctg atcgagccga tctttgccta
     2461 cgagggcatc ggcagcgagc tatggcgctc catccagcag cgggactacc cgatgctgca
     2521 gggcatcttc ctggtcgtga cggtctcggt ggtggtcgcc aacctgatcg ccgacctgtt
     2581 ctacagccgg ctcgatccac gaatccgcac cggaggaggt gagtagtcat ggctgaagcg
     2641 gtcgttggga cgggcgcgcc ttcgccggga gtggacagag ccgctgccag gcagcgcaag
     2701 agctttctac gcgtctttat cgagcgcaac aaggtcggca tgatcggcta tgtcatcttc
     2761 ggcgcgatcc taatcatgtc gttcatcgga ccgctcttcc tgccggacga gaacccgacc
     2821 gacgtcgggc tcgtctacgc cccgccatca tgggaccacc cgctcggtac ggactggcgc
     2881 gggcaggatg tgctctacca aatcatcaac ggcggggcgg gcctgctgat cgtgagcatg
     2941 attgcggcgg gcgttgccac ggcggtcgcc gtgatcttcg gggcgctggc ggggctcatc
     3001 ggcggccggc tcgattcggt cgcgctgatc ctggcggacg tcgtgctcac ggtcccgttc
     3061 atcatcctgc tcggcgtgct cgccgtcttc gtgcggctcg acaacatctt cctgctcgcg
     3121 ctggtgctcg ctgccgtcgg ctggccgacc ctgttgcgag cggtgcgcgc tcaggtgctc
     3181 tcgctcaagg agcgggagta tgtcgaggcg gcgcggatgc tcgatctccc gatgcggcac
     3241 gtgctcttcc gcgaggtcct gccgaacatg atgccgtaca tcatcatcaa cttcgtgctc
     3301 ggcatgacca acgccatcta cgggcagatc atcctgtact acctcggcct ggtgccgtta
     3361 cagggcgaca actggggcct gatgatccag cagatccagt ccaagcaggc ctactcgctc
     3421 aacgatgcct ttgtctacgt gatggcgccg atcgtgatga tctcgctgct ccagctctcg
     3481 ctggtcctga tctcccgtac gctcgaagac gtcttcaacc cccgattgcg cgaggtgtag
     3541 ctcatggcaa ccagcacaca ggcggcccag gaccgggaag cgaaggaccg aattcgcggg
     3601 accgccgagt cgaccgcgcc gacgatcctc gaggtcgacg atctctgggt cgagtacaag
     3661 acgccggccg gctggatgca ggcggtacgc ggcgccaaca tgtccatccg caagggggag
     3721 gcggtcgcgc tgatcggcga gagcggctcc ggcaagtcga cgctgggctt cgccatcctg
     3781 cgcatgctcg tgcgcggcgc ccgggtgagc cgaggctccg tgcgcttcac caccagcaag
     3841 ggagagacgc tcgacctcct caagatgtcg gacagggagt tccgcagagt gcgctggaag
     3901 gagatcgcga tgaccttcca ggcggcgcag agctcgctca acccggtgat gaaggtcaag
     3961 gacaccttcc aggaaaccgc gcgcgcccat ggatggcgca acaggggcga ggtgcgggag
     4021 cgcactctgg atctgctcca gaaggtgcag ctggacccgg atcgcgtcta caacgcctac
     4081 ccgcacgagc tctccggcgg catgcgccag cgcgtctcaa tcgcgctgag tctgctgctg
     4141 cagccgacgc tcatcatcct cgacgagccg accaccgcac tcgacatcct cacgcagcgg
     4201 gcgatcatcg acgtggtccg caacctgcgt gaggagctcg acttcacgat gatcttcatc
     4261 tcgcatgacc tctcgctggc cgccgagctg gccgatcggg tcgcgacgat gtacgccggg
     4321 gaggtggtcg agtacggtcc ggtgcgcgac gtcttctacg agccccagca tccctattcc
     4381 gtcggattaa tgaatgccgt gccgcccatc gccggtgagg agttcacccc cctgacggcc
     4441 atcccgggat cgacaccgaa cctgctcaac atcccgagcg ggtgtccgtt ccacccccgg
     4501 tgtccgttcc acaccgacat ctgtatcacc gaccatccac ctctggagga ggttgcaccg
     4561 tcgcacctcg ccgcctgtca ccaccatgac gaggtggaca aggaccccga acgatgggag
     4621 tcgatcgacg atgtggagga agcggtcggg tagggacata cgtcgaccgg ttttcttgtg
     4681 caggctgatc atccataggg gcggacccgc gaagcagttg tccgcccagc cgcccaaagg
     4741 gtggcgccag tcaaatgccg tcacttcgac cggagcggcg aaatctctcc cgtaatcgga
     4801 ctcagtcact cgccaggcaa gagatttctc gacacgctcg gcgcgtgccg cgcctcgcgg
     4861 ctcgaaatga cagattgagg agcaggacag ggcccatgac agaaccgatc atgcagctgg
     4921 agcatctgac ccgccgcttc aagacgccga agggcatcat caaggcggtg gatgacgtct
     4981 cgttcaccct ctacccgggc gatatcctct gcctcgtcgg cgagagcggc tccggcaaga
     5041 cgaccgcggc tcgcatggcg ggcggcctgc tcaagccgag cgaggggcgg atcatcttcg
     5101 agggcaagga catcgcggag atgagcaccg aggagtttgc cgagtaccgc aagcaggtcc
     5161 agatcatcca ccaggacccc tacgcctcgc tcaacccggt ccacaccatc ggcgacaccc
     5221 tgcgcgcacc gctgctctac cacaatgtcg tgaagaacag gaccgaggcg acccggcgcg
     5281 ctggcgcgct gcttgagctc gtcgacctgc gtccggtcga gaacttcatc gacaagttcc
     5341 cgcaccagct ctccggcggg cagcgccagc gcgtctcgat cgcgcgcgcg atggtgctca
     5401 acccccgggt gatcatcgcc gacgaatccg tctcgatggt cgacgtctcg atccgcgtct
     5461 cgctgctcaa caccatgctc aagctgcgga acgagctggg ggtgaccttc ctcttcatca
     5521 cccacgacct cgccgttgtg aagtacttcg cctgggaagg gcgcatcggc gtgatgtacg
     5581 tcggcaagct gatcgagtac gggcgcacgc cgaggctgat cggtcggccg ctgcagccat
     5641 acacccgcgc gctggcggcg gcgattcccg aggcggatcc ggacctgacc cgatccaaga
     5701 gtaagttgca gctgcgcagc ctggatatcc cgaacctcaa cgatctgccc gacggctgtg
     5761 tctttcaccc gcgctgcccc tactgggtgc agggcctgtg cgacgtggag atgccgccgt
     5821 tggtggacgt gggggagggg cgactggtcg cctgccacgt cgtgcagcgg aatgtcgagg
     5881 cgggcggtga cggccgggat cttgaggcca cccgcgagtc gatgctcgcc cacgccgccg
     5941 actgagccgg ggcaaggtga tcaggtcatt gctggatcat ggagtgtagc ggcaggatgt
     6001 gatagccgac tgccgtctca accattggat tggtgggcat accgcacaac ggccggctcg
     6061 gtggctcgcg gcggagacat tacgctatac tgtgcatcgt tcctcgaacg attcagagtc
     6121 gcacgtccac caaggaggtt ggaatgtcga aggatacctt tgaagaaacg catcgcagca
     6181 tgctgcaggc accgatgtcg cgccgtcgcg cgatgggcgt gggtgccgga gcggtcggag
     6241 cagcgatgct cacaggcgca cccctgccag ccggcttcgg ccgggtggtg atggcgcagg
     6301 acggcggcgc cgagttccat tcggcctacc cgtaccagca gccgccgacc ggtcacttca
     6361 acacgttcgt gaccaacgcc atcctgcccc cgatgcctca gggcggcacc gtctatggcg
     6421 agctcatcta cgagccgctc ggcatgttct actgggggac cggtgagtgg ctgccgctgc
     6481 tggccaccga gtggtcgttc ctcgatgacg gcgaaacctt cgagatcaag ctgcgcgagg
     6541 gggtgaactg gagcaacgac gagccgttca cggccgccga tgtcgatgcc accctatggt
     6601 gcctgcgcat catgtccaac acgctgtggc agtacgtcga ccaggtcaac gtcgtcgatg
     6661 actacaccgt gcagctgcat atgactacgc cgtcaacggt cgtcgagcgc tacgcgatcc
     6721 gcgtggcctt gccacggccg gcctcggtct acggtgagtg ggcggaccgc gccaaggagc
     6781 ttttcgaggg cggcttgacc ctggacgatc cggagggcaa gcagctgctg gaccagttca
     6841 accagttccg cccggatccg gcaaacctgc cggtgaccgg cccgttcacc atcgacccga
     6901 acagcatcac cagtgcccag ttgacgatgg ttcggaacga gaacgcctgg aacgctgacc
     6961 aggtgcagtt cgatcggatc gtcaacttca acggcgagac ggacaccatc acctcggtgg
     7021 tgctggccgg agacgtcgac tacgcgaccc acggcttcgc gccggccacc gagtccgagt
     7081 tcctgaatca gggcatccgc gtgctgcggc cacccgtcta ctc
//
LOCUS       DASYWL010000133         5860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_118804, whole genome shotgun sequence.
ACCESSION   DASYWL010000133 DASYWL010000000
VERSION     DASYWL010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5860
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118804"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..704)
                     /locus_tag="VGR22_04705"
     CDS             complement(111..704)
                     /locus_tag="VGR22_04705"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEV2127902.1"
                     /translation="MANANQTHEMLPVPAPFTGRREIDLDAVREMVSHFHFDDYEEAQ
                     ELILSALQEINEVYGWVSPEATEIVARHLNTTPERVFSLLTFYADFRTARRGHHHMLI
                     CHGMACYVHGSQRLVQHMQDDFGVQDGDTTTDGNMSVQVVNGCLGVCDQAPVVKIDDT
                     YHGSVDAETLTDLVNDAVANHTALTENEETHGPRGTN"
     gene            complement(863..1234)
                     /locus_tag="VGR22_04710"
     CDS             complement(863..1234)
                     /locus_tag="VGR22_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127903.1"
                     /translation="MKQIVLKRMERCSVCHRHFSPDDVHILNRQPDMWTMMVECTDCH
                     SRNFVAALMNDGDPGEARLALRELSDTIAQELHPAKQEIDPADTLPDGEPVSAADVVD
                     MHLFLDEFDGDFKLHFQQRGH"
     gene            complement(1271..1846)
                     /locus_tag="VGR22_04715"
     CDS             complement(1271..1846)
                     /locus_tag="VGR22_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEV2127904.1"
                     /translation="MIETTVDSIRVSLVTQQRVVILKEIDGDRHLPIWIGAYEAEAIA
                     MELQGVTPTRPLPYDLMQTLLRELNATVDQVIIGDLNEQVFYARVVVSTSESTFEVDS
                     RPSDAIALAVRTGSRILVDEAVMDSAGVAVEEDEDPAALSASSFSSEPSASPGEPEET
                     DSAPAPTDEQLSVFRDFINSLDLDDFGKKQS"
     gene            complement(2048..2809)
                     /gene="lepB"
                     /locus_tag="VGR22_04720"
     CDS             complement(2048..2809)
                     /gene="lepB"
                     /locus_tag="VGR22_04720"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEV2127905.1"
                     /translation="MTFDDRSIESQGPHDRPPRTTDAVDTSTASDDDTPTRVDARPRA
                     QKVSVIREIVETLLLALIIFVAVRAVVLNFRVDGLSMTPNLENSEMLLVNRNAYLSFD
                     SWALVDWLPGVEHEEANVVEPFGQPERGDIVVFDAPVANASQPYIKRVIGLEGETVVI
                     RNDGVYIDGQELNEPYLEGAATFCSGGRDCPAVGVPEDHVFVLGDNRDNSQDSRAFGP
                     VPVDEIIGKAWLSYWPVDEVGTVPHYEYPEISDTP"
     gene            complement(2969..3235)
                     /gene="tatA"
                     /locus_tag="VGR22_04725"
     CDS             complement(2969..3235)
                     /gene="tatA"
                     /locus_tag="VGR22_04725"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEV2127906.1"
                     /translation="MGPIGVPELSIILVIIVIIFGVGKLPEIGGALGKGIREFKSESG
                     VGDKDEQLTEGNSEKASSLTANTTTRSEEVGVRAERREVRADDI"
     gene            complement(3371..3592)
                     /gene="secG"
                     /locus_tag="VGR22_04730"
     CDS             complement(3371..3592)
                     /gene="secG"
                     /locus_tag="VGR22_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEV2127907.1"
                     /translation="MDTALNIITILVAILLITMIMVQTKGSSFGTGFGGGGSMYTTRR
                     GFEKTLFTATIGVAVFFVLLSIINSFALS"
     gene            complement(3692..4468)
                     /gene="tpiA"
                     /locus_tag="VGR22_04735"
     CDS             complement(3692..4468)
                     /gene="tpiA"
                     /locus_tag="VGR22_04735"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEV2127908.1"
                     /translation="MTSTARIPLVLGNWKMHTSLAEAHELARMTGEAAARHPEVEVGL
                     LPPYPWIVPLAEALGTEPAVRVGAQDIAAETAGAFTGDVAAEMLAPYVRFMLVGHSER
                     RHGHGETDAEVRAKLDAVYRVDRIPVLAVGETEEERQAGRAVDVVTRQLAAALNDRPS
                     AALETLVVAYEPVWAIGTGQAATPDDTSEMAGVVRSLLHQLDQAAAERTRILYGGSVK
                     ADNARAFFAVPDIDGGLVGGASLDARAFTGIIAAAGVDGG"
     gene            complement(4465..5661)
                     /locus_tag="VGR22_04740"
     CDS             complement(4465..5661)
                     /locus_tag="VGR22_04740"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEV2127909.1"
                     /translation="MSKRSIRSANVAGKHVLVRVDFNVPIEDGRITDDTRIRAAVPTI
                     TWLVEHGAKVILASHLGRPKGKVVDELRLAPVGRHLSTLLGRSVATMTSITGSDVTEA
                     VDAMQPGDVILLENLRFDPGEEANDSEFAQQLAELADLFVNDAFGAAHRSHASTTGIA
                     NYIPAYVGMLVEHEEKMLSRLLESPEQPFVAIVGGAKVSDKIGILQHLVGKVDTMLIG
                     GGMANTFLAAQGHEVGTSLMEEDQVDTARDILRAADERGVKIVLPTDVVIAASIDAPR
                     GENVPVSEVPNDKAIFDIGSETRREFFRIISGAKTVLWNGPLGVAERAPFAMGTRRLA
                     EAVAICDGYTVIGGGDSIAAVEQIGLAQNIDHVSTGGGASLEFLEGKTLPGIAVIPDA
                     AEASAS"
BASE COUNT         1010 a         1805 c         1926 g         1119 t
ORIGIN      
        1 cccccacgct cacgacggta cggttgccct cccagagatc actccagcgc ctgcgggccc
       61 ggccccggaa tgcttcgtac tcttcccgtg aggtgaggcg gggcaggtgc tcagttggtg
      121 ccgcgtggac catgggtctc ctcattctcg gttagggcag tgtggttggc gacagcatcg
      181 ttcaccaggt cggtcagggt ctcggcgtcg acggaaccgt ggtatgtgtc gtcgatcttg
      241 acgaccggtg cctgatcgca gactccgagg cagccgttca cgacctgcac gctcatgttg
      301 ccgtcggtgg tggtgtcacc atcctggaca ccgaagtcgt cctgcatgtg ttgcacgaga
      361 cgttgcgagc cgtggacata gcaggccatg ccgtggcaga tgagcatgtg gtgatggccg
      421 cggcgcgcgg tgcggaaatc ggcgtagaac gttaagagcg agaagacgcg ctcaggggtg
      481 gtgttgaggt gccgagcgac gatctcggtt gcctccggcg atacccaccc atagacctca
      541 ttgatctcct gcaatgccga gaggatgagt tcctgcgctt cctcgtagtc gtcaaagtgg
      601 aagtgggaaa ccatctcacg cactgcgtcg agatcgatct cccgccggcc ggtaaatgga
      661 gccggcaccg gaagcatttc atgcgtctga ttggcgttcg ccactcgacc gtccttcctg
      721 gcgggcgtgc aacgcgggcg cccacgtcga caccgcagcg cgctgatcac aatgcatttt
      781 cgcggggaag tatagcagca gggcagacgc gatccgttcg cgtctgccct tgctcgattc
      841 gcagattccg ttgccagaga agctagtgcc cgcgctgctg gaagtggagt ttgaaatcgc
      901 cgtcaaactc gtcgaggaag agatgcatgt cgacgacatc ggcggcggag acaggttcgc
      961 cgtcaggcag cgtgtcggca gggtcgatct cctgcttcgc cgggtgcagc tcctgagcga
     1021 tggtgtcgct tagctcacgg agggcgagac gggcctctcc cggatcaccg tcgttcatga
     1081 gcgcagcgac gaagttgcgc gagtggcagt cggtgcactc gaccatcatg gtccacatgt
     1141 ccggctgtcg attgaggata tgcacatcgt ccgggctgaa atgccggtgg cacacggagc
     1201 agcgctccat tcgcttgagg acgatctgct tcatggtgac atcaggttcc atgggttcta
     1261 ggctatctct ctaggattgc ttcttgccga agtcgtcaag atcgagcgag ttgatgaagt
     1321 cgcggaagac ggagagttgc tcgtctgttg gagcgggcgc ggagtcggtt tcctccggct
     1381 cccccggtga ggccgagggt tccgagctga acgaggaggc tgagagagca gcgggatctt
     1441 cgtcttcctc caccgcgaca ccggcgctgt ccatgacggc ctcatcgacc aggatgcggc
     1501 tgccggttcg aaccgcgagc gcgatcgcgt cactcgggcg ggaatcaacc tcgaaggttg
     1561 actcgctggt ggagaccacc acacgcgcgt agaagacctg ctcgttgagg tctccgatga
     1621 tcacctgatc gacagttgcg ttgagctcgc ggagcagcgt ctgcatgagg tcgtagggga
     1681 gtggccgcgt cggcgtcaca ccctgcagtt ccatggcgat ggcttcagcc tcgtaggcgc
     1741 cgatccagat gggcagatgg cgatcgccgt cgatctcctt gagaatgacg acacgctgct
     1801 gcgtgacgag gctgactcgg atgctatcga cagttgtttc gatcatggcg ttttcacctt
     1861 tcggtgccat tgtgcagagc caacccggag gtgaagttgt tcgctgtgag cggggtggct
     1921 tcgctgcggg atcggcccca tgctgccatt gtacacagcc ctgccgcctg ccgacatacc
     1981 cgccacgggc agtcgctaca gctcgagagc gtgctccgct ctgttgagac gcatgacagg
     2041 tgccacgtca gggagtgtcg gaaatctccg gatactcgta gtgcggcacg gtgcccactt
     2101 cgtcgactgg ccagtagctc agccacgcct tgccgatgat ctcatcgacc ggtaccggcc
     2161 cgaacgcgcg ggagtcctgg ctgttgtccc gattgtcgcc aagcacgaag acatgatcct
     2221 cgggaacccc gaccgcagga cagtcgcgac cgccggagca gaaggtagca gctccttcaa
     2281 ggtagggctc gttgagttcc tgcccgtcga tgtagacgcc atcgttgcga atcacgaccg
     2341 tttcgccctc caggccgatg acccgcttga tgtagggttg gctcgcgttg gcgaccggcg
     2401 cgtcgaagac aacgatgtcg ccgcgttcgg gctgcccgaa cggctcgacg acgttcgctt
     2461 cctcgtgctc gacgcctggg agccagtcga ccagtgccca gctgtcgaag ctgaggtagg
     2521 cgttgcgatt gaccagcagc atctcgctgt tctccagatt gggcgtcatg ctgagcccat
     2581 cgacacggaa gttgagcacc acggcacgga cggcgacgaa gatgatgagt gccagcagaa
     2641 gcgtttcaac aatctcccgg atcaccgaga ccttttgcgc tctcggtcgg gcgtccaccc
     2701 gtgtcggtgt gtcgtcgtca ctggcggtgg aggtgtcgac ggcgtctgtg gtgcgcggtg
     2761 gacggtcgtg aggaccttga gactcgatcg agcggtcgtc aaacgtcatg cgtgggcggc
     2821 cttctggcga acgggtggcg tgccgttatc tggagggaat gatactaccg cgtctatccc
     2881 ggcatcggta ttcaggcgcg cgggacgacg aacgcccgag gtcatcaatc ccccgggcgt
     2941 tgccttgtgc gtgatgtcgt gcgatccgct agatgtcgtc ggcgcggacc tcgcggcgct
     3001 ctgcgcgcac accaacctct tcagagcggg tagtggtgtt cgcggtcagc gaagacgcct
     3061 tctcgctgtt gccctcggtg agctgctcat ccttgtcgcc aacaccggac tcggacttga
     3121 actcgcggat gcccttaccg agcgcaccac cgatttccgg aagcttgccg acgccgaaga
     3181 tgatcacgat gatgacgaga atgatgctaa gctcaggaac tccgatcggc cccatgggcg
     3241 cacctccata cctgttgaac gattccgtcc tcgcgactca cgagagcgga agtgtgttcc
     3301 ctgtctggtg agccgaagct gcctaattct aggcagtcgg gctccagaaa tcaacgtccc
     3361 gatccggctg tcaggagagg gcgaagctgt tgataatcga cagcagaacg aagaaaaccg
     3421 cgacgccgat ggtcgctgtg aagagtgttt tttcgaaacc gcgtcgcgtg gtgtacatcg
     3481 acccaccgcc gccgaatccg gttccgaacg acgatccctt ggtctggacc atgatcatcg
     3541 tgatcagcag gatcgcgacg agaatcgtaa tgatgttcag cgcagtatcc acgtaaagct
     3601 gtgctcctca gcaaatcgcg aatcctctca tcgtagcgtc caccactcta ccacagcggc
     3661 gggcatgggt cggtcctgcc ggaaccttgg ttcagccgcc gtcgacccca gccgcagcga
     3721 tgatgcctgt gaaggcgcga gcgtcgagac ttgcgccgcc gaccagacca ccgtcgatgt
     3781 ccggcacggc gaagaacgcc cgcgcgttgt cagccttgac gctgccgccg tagaggatgc
     3841 gtgtccgctc ggcagcggcc tggtcgagct gatggagcag gctccggacc acgccggcca
     3901 tctcggatgt gtcgtcagga gtggccgcct ggcctgtgcc gattgcccag accggctcgt
     3961 aggccaccac cagggtctcc agggcggcgg acgggcggtc gttcagtgcc gccgcaagtt
     4021 gccgcgtgac gacgtcaacg gcgcgtcccg cctggcgctc ctcttcggtt tcgccgacgg
     4081 caagcaccgg gatacggtcg acgcggtaga ctgcatcgag tttggcgcga acctccgcat
     4141 cggtttcacc gtggccgtgg cgccgttccg agtggccgac gagcatgaag cggacatacg
     4201 gggccagcat ctcggcggcg acgtcgccgg tgaaggctcc tgcggtttcc gcggcgatgt
     4261 cctgcgcgcc aacgcggacc gcgggctcgg taccgagcgc ttcggctagc ggaacgatcc
     4321 atggataggg cgggagcaag cccacctcaa cctccgggtg ccgcgccgcg gcctcgcctg
     4381 tcatgcgtgc cagctcgtgc gcctctgcca gcgaggtatg catcttccag ttgccgagca
     4441 ccagggggat acgcgcagtg ctggtcacga cgcagatgcc tctgcggcgt ccgggatcac
     4501 ggcgataccg ggaagcgtct tgccctcgag gaactcgagc gatgcgccgc cgccggtgga
     4561 gacgtggtcg atgttctggg ccagaccgat ctgttcgact gcagcgatcg agtcgccgcc
     4621 accgatcacg gtatagccgt cacagatcgc gaccgcctcg gcgagccggc gggtgcccat
     4681 ggcgaagggt gcccgctccg caacgccgag cggcccgttc caaagcacgg ttttcgcgcc
     4741 agaaatgatg cggaagaact ctcgtcgcgt ctcgctgccg atgtcgaaga tggccttgtc
     4801 attcggtacc tcggacactg gaacgttctc accgcgtggg gcgtcgatgc tggccgcgat
     4861 gaccacgtcg gtcggcagca cgatcttgac cccccgctca tctgcggcgc gaaggatgtc
     4921 acgggcagtg tcgacctggt cctcctccat gagggaggtg ccgacctcgt gcccttgggc
     4981 ggcgaggaac gtattggcca tgccaccgcc gatgagcatg gtgtcgacct tgcccacgag
     5041 atgctggagg atgccgatct tgtcggaaac tttggccccg ccgacgattg ccacgaacgg
     5101 ctgctcgggc gattcgagca ggcgcgagag catcttctcc tcgtgctcca cgagcatccc
     5161 gacgtacgcg ggaatgtagt tggcgatgcc cgtcgtcgac gcgtgggagc ggtgcgcggc
     5221 gccgaaggcg tcgttgacga agaggtctgc caactcggcg agctgctgcg cgaattcgct
     5281 gtcgttggct tcctcgccgg ggtcgaaccg caggttctcg agcaggatca catcgccagg
     5341 ttgcatggcg tccaccgctt cggtgacgtc ggatccggtg atcgacgtca tcgtggcgac
     5401 cgagcgtccc agcagagtcg acaggtgcct gccgacgggc gcgagccgca actcgtcaac
     5461 gaccttgccc ttggggcggc cgaggtgcga tgcgaggatg accttcgcgc catgctcgac
     5521 gagccaggtg atggtcggta ccgccgcgcg gatgcgggtg tcgtcggtga tgcgcccgtc
     5581 ctcgatcggt acgttgaagt cgacgcgcac cagaacgtgc ttgccggcga cgttggccga
     5641 gcggatggag cgtttgctca tgcgtctttc ccccactgga gaggcaaccg ggcagcgagg
     5701 ggctcgtcgc tgcccgtgcc aggcgtgcgg gcgggcacac ggccctcccc taggcgaagc
     5761 cctgctcaca gatgaccgcg gtcaagtccg cgatgcggca ggagtagccc cactcgttgt
     5821 cgtaccaggc gatggccttc accagccgat caccggaaac
//
LOCUS       DASYWL010000134         4003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_194226, whole genome shotgun sequence.
ACCESSION   DASYWL010000134 DASYWL010000000
VERSION     DASYWL010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194226"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VGR22_04745"
     CDS             <1..621
                     /locus_tag="VGR22_04745"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV2127910.1"
                     /translation="SRDDLIALFGIQTLVRGLIGLVIQPSMQRLMEGIRLGTLDFMLT
                     KPADSQLLASVAQVNIASTANIIVGLGVLIVALVRLGSNVSIVEALTFALVLVAGAVI
                     IYSFLLMLSTLAFWFVRLDNILVIFETLFGNAGAWPITIYPNWLQVSLTFLVPVAFAV
                     TIPAQSLTNRLGGLAVLGTVALAVAFAAAARWFWRFGLRHYTGASA"
     gene            complement(1006..1428)
                     /locus_tag="VGR22_04750"
     CDS             complement(1006..1428)
                     /locus_tag="VGR22_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127911.1"
                     /translation="MAGGFSRRQIKTFRGTATLIAIGASLFQGLIAFGSLKAQAWSLN
                     DTGQGIIVLMLVLVVLAAMALATMAMLVSPETATFLGIGALVLDLVIVTIALRSAADP
                     TGNESLTNAGLLIAPAVVMVVTGVVLTKLEGGRIRFFG"
     gene            complement(1680..1928)
                     /locus_tag="VGR22_04755"
     CDS             complement(1680..1928)
                     /locus_tag="VGR22_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127912.1"
                     /translation="MYMLCSVEGIAIPRWPRFAWWFAGFVVLVAASNLLGQVAGPPEA
                     VAISLIWLAATGAMVPLVWIGRRDLAAPSVLPPSRIAP"
     gene            complement(2049..3020)
                     /locus_tag="VGR22_04760"
     CDS             complement(2049..3020)
                     /locus_tag="VGR22_04760"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV2127913.1"
                     /translation="MIEISIAAEGVFGLTWPAWKRLVRTVEDLGFAGLYLSDHFVLPN
                     PPDYPSLEVMLALGWAADHTERVRLGQRVSPVSFRDPVLLARQTAAIDDLSGDRMSLG
                     LGAGWMEREHAMFGYDLGDIPTRFARLEESLEIITRLLRSNEPVNFEGCFYRLENAVL
                     PPPRRPGGPPIVIGGIGPKRTLPLVARYADNWNAGNLSPDQLAERSAMLDGLIVAAGR
                     RPEDIIRTFNIPVVCWRTPAELEARIGAVRRVSNEVRDMSTDQLVEWLRGWPAILGTL
                     EEVIEQIRAYEAVGVTEIEAKWPAADDIEGLEILATEVLPQVTSSSA"
     gene            3153..3977
                     /locus_tag="VGR22_04765"
                     /pseudo
     CDS             3153..3977
                     /locus_tag="VGR22_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897899.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          687 a         1383 c         1240 g          693 t
ORIGIN      
        1 tcgcgggatg acctgattgc gctgttcggg atccagacgc tcgtccgggg cctgatcggc
       61 ctggtgatcc agccaagcat gcagcggctg atggagggga tccggctcgg cacgctcgac
      121 ttcatgctga ccaagccggc cgattcgcaa ttgctggcga gcgtcgcaca ggtcaacatc
      181 gcctccaccg cgaacatcat cgtcgggctc ggggtcctga tcgtggcgct ggtccggttg
      241 ggctccaacg tctcgatcgt ggaagcgctg accttcgcgc tcgtgcttgt cgctggcgcg
      301 gtcatcatct actcgttcct gcttatgctc tcgaccctcg cgttctggtt cgtccgcctc
      361 gacaacatcc tcgtcatctt cgagacgctg ttcggcaacg cgggcgcctg gccgatcacg
      421 atctatccga actggctcca ggtctcgctg acgttcctgg tgccggtcgc cttcgcggtg
      481 acgatcccgg cccagagcct gaccaaccgc ctcggcgggt tggccgtgct gggaaccgtt
      541 gcgctggccg tcgcctttgc ggccgccgcc cgctggttct ggcgcttcgg cctccgccac
      601 tacacgggcg cctccgcctg agcgcacgtc gctcacaacc ccggcgattt gatccggtat
      661 ccggcccgac ccggccttgg tcgatcctgc cgctcctgtc gcacgtcgcc atccagcctc
      721 gctgccatcg cgcacgttgt tccaggattg ccggcctcgc cagcaagtgc ccgccatcct
      781 gaacagtgcg tagaaggcct cgtctgaaca ctcgtgccgg ccacctacgt tgatcggcgc
      841 gcggctccgc caccattcgc acgttggttg tcatcccgac gacatgcggg gttggaagcc
      901 cgccctgggc aaaggctgga gagacacacc ggcgtcccag cgcctttcat cctgggcgca
      961 cgtccatcct ccggagcccg tcggcatcca cggtagcggg gaagatcagc cgaagaacct
     1021 gatccttccg ccctccagct ttgtcagcac cacaccggtc accaccatca ccaccgctgg
     1081 cgcgatgagc agtccggcgt tggtcaggga ctcgttgccc gtcggatcgg cggcgctacg
     1141 caacgcaatc gtgacgatga cgaggtcgag cacgagcgcg ccgatgccaa ggaaggtcgc
     1201 cgtttccggc gagacgagca tcgccatcgt ggcgagggcc atcgcggcga ggacgaccag
     1261 caccagcatc agcacgatga tgccctggcc ggtatcgttg agcgaccagg cctgggcctt
     1321 gagactgcca aaggcaatca ggccctggaa caggctcgct ccgatcgcga tcagcgtcgc
     1381 cgtcccccga aacgtcttga tctgtctgcg ggaaaatccg cctgccatca actgcccttt
     1441 ctcttttcgc cgtgccggtt cccggatgtg aacactcgac atggacagcg agaccgcgcg
     1501 cagcccgcga gatcgacgcg tcttcgcgcc cagcggcccg ggccctccac tggagacggc
     1561 acctgcatca aagcgaggat catcgacagc gcgtgtgcct ggaacgatat ccgctcatct
     1621 ccgtcatcgg caacgcacca cgatagcgat cacgatagga atccagaacg tcgctccagt
     1681 caaggggcaa tccgcgacgg gggtaacaca gatggtgctg caagatcgcg ccgcccgatc
     1741 cacaccagcg gcaccatggc accggttgca gccagccaga tcagcgagat ggcgacggcc
     1801 tcaggtggac cggcgacctg tccgagcaga ttgctcgcgg caacgaggac cacgaagcca
     1861 gcgaaccacc aggcaaaccg tggccacctc gggatggcga tgccctccac ggagcagagc
     1921 atatacatga ggaggctcta agaacctgcg tcaatagggt caaagcgctg tgcatgacaa
     1981 tcggggcagt gacgtcccga tcacgtcaat cggcatcagg aggtatgccg tcagtctcct
     2041 ctctcgcctc acgctgacga cgacgtcacc tgcggcagta cctccgtggc caggatctcc
     2101 agcccctcga tgtcatccgc cgctggccat ttcgcctcga tctccgtgac gccgaccgcc
     2161 tcatacgcgc ggatctgctc gatgacctcc tcgagggtgc ccagaatcgc aggccagccc
     2221 cggagccact ccaccagctg gtccgtcgac atgtcacgga cctcgttcga gacccgccgg
     2281 accgctccaa tgcgagcttc tagctcggcc ggtgtgcgcc aacagaccac agggatgttg
     2341 aaggtccgga tgatgtcctc cggccggcgt cccgccgcga cgatgaggcc gtcaagcatc
     2401 gccgaccgct ccgccagctg gtccggcgac aagttccccg cgttccagtt gtcggcgtag
     2461 cgggcgacga gggggagggt gcgcttgggg ccgatgccgc cgatcacgat gggcggaccg
     2521 cccggtcggc ggggcggagg gagcacggcg ttctccaggc ggtagaagca cccctcgaag
     2581 ttgaccggct cattggagcg gagcaggcgc gtgatgatct cgagcgactc ctccagccgg
     2641 gcaaagcgcg tcgggatgtc gccaaggtcg tacccgaaca tcgcgtgctc gcgttccatc
     2701 catcccgccc cgagcccgag actcatgcgg tcgccgctga gatcgtcgat ggccgcagtc
     2761 tgccgggcga gcagcacggg atcacgaaag gagaccggcg agacccgctg gccgagcctc
     2821 acacgctcgg tgtggtccgc cgcccagccg agcgcgagca tcacctccag cgacggatag
     2881 tcgggcgggt tgggcaggac gaagtggtcg gacagataca ggccggcaaa gccgagatcc
     2941 tcgactgtgc ggaccagccg cttccacgcc ggccaagtca gcccgaacac accctcagcg
     3001 gcgatcgaga tctcaatcat cgctgcgctc cttaccatga gcatgttaac gatcagcgcc
     3061 gtgctgtacg cgagtcagct aggaaccagc atagggcacg cgctccggcc acgacatgcg
     3121 gcctgtgtac tttcgatgag aggagcgttc gcgtgcccgc cgtcccatcg tccattctcg
     3181 tgcccatctg ggaccagttc gctgcactgt tgccatcacg gtaggtggtc catccgttgg
     3241 gctgccaccg gccacgcatc cctgatcgca tcgtgttcga caagctcgtg caggcgctcg
     3301 tctttggctg cgcccaccag cgaatcgccg acgagacgcg ttcggccacc acgctgcggc
     3361 ggcgccggga cgagtggatt gcggacggca tcatggatcg gctccagggg ctcgtgttgg
     3421 aagcctatga ccgtatgatc gggctggacg tgagacatat cgctatcgac tgctgcatca
     3481 ccaaggctcc ctgcggcggc aaggtggcgg gccgcagtcc cgtcgatcgg ggcaagcatg
     3541 gcctcaagcg ctccctggcc gttgatgcgg ctggcattcc actcggtgcc attgccggcc
     3601 ctgctaaccg gcacgatacg accctgctag gaccgaccct tggtgccctg gcagtcaccg
     3661 acgctctcat gactgtccat ctcgaccggg gctacgactc gagtgccgcc cgcgccctgc
     3721 tcgcagccta caggctgacc tctgacatcg cgttaagggg tagcccgacg cccatcaccg
     3781 tgggtcagcg gtgggtcgtc gagcgcacca tcgcctggac gaacgcccac aagaagctcg
     3841 tctggtgcac cgagcgccgg gacattgtcg tcgccttctg gatcgccttc tcggcggtgc
     3901 tcatcatcgt tggccgcctt gtgcgggaag gctggatgcg ctaccgctgg gaccggcgcc
     3961 cgcgccgaag accgtgatgc cgagcctatt ggcgcaagct cta
//
LOCUS       DASYWL010000135         7659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_306605, whole genome shotgun sequence.
ACCESSION   DASYWL010000135 DASYWL010000000
VERSION     DASYWL010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7659
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306605"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /gene="larC"
                     /locus_tag="VGR22_04770"
     CDS             complement(<1..1004)
                     /gene="larC"
                     /locus_tag="VGR22_04770"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HEV2127914.1"
                     /translation="MKIAYFDPFSGASGDMTLGALVDAGLSLEQLTSELGKLRLGGYR
                     VRAERAGQHGIHGTRVTVEVTGEVHHHRDWATIRDLIMNAGFATPVQAAALAMFERLA
                     KAEAKIHDSGLDQVHFHEVGGIDAIVDICGTAVGLSLLGIEAVFSGPPQLGHGFASSA
                     HGTIPVPAPATLELIADAGTPTATPILAMQEHPAELLTPTGATILTTLATFTRPSFAP
                     SVIGYGFGQKELPWPNALRVWIGEMADADATDGEVVIETNIDDMSPQFYEVLTERLFA
                     EGALDVWTTAITMKKGRPATKVSLLAPASRQAQLEHVLMFNTTTLGTRSWSIERTKAG
                     R"
     gene            1814..2695
                     /locus_tag="VGR22_04775"
     CDS             1814..2695
                     /locus_tag="VGR22_04775"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEV2127915.1"
                     /translation="MMAEQYGGHSMIAPLRRVLVRKPAPPAGEGDAARFNYPNQPNHD
                     VAVREHDAFVRKLQELGVDVIEAEAAATGELDAIFVYDPSFTTDGGLLLMRPGKVARA
                     SEVEMAREAAFALDIPIIGEIQEPGTVEGGDMLWLDQNTLAIGEGYRTNAAGIDQVQI
                     FLRPVGVDVVRMALPYWHGPDECLHLMSLISPVAEKLAVVYKPLMSVPFVQFLEDEGW
                     SFVEIPDEEFATQGSNVLAIEPGKVLMLKDNPVTIDRLRKAGVEVHTYTGDEISHNRA
                     GGPTCLTRPILRRTLTA"
     gene            2832..3131
                     /locus_tag="VGR22_04780"
     CDS             2832..3131
                     /locus_tag="VGR22_04780"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEV2127916.1"
                     /translation="MFWPAGIERTDRVKLELDEGPLYGVTNDLAARLYQHRNVGDNTF
                     AGQYRTHRLVYVEELPRIDDAIAREKQLKGWKRDRKVALIDSMNPVWRDLAEDWG"
     gene            complement(3298..3395)
                     /locus_tag="VGR22_04785"
     tRNA            complement(3298..3395)
                     /locus_tag="VGR22_04785"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3359..3361),aa:Sec,seq:tca)
     gene            3524..3937
                     /locus_tag="VGR22_04790"
     CDS             3524..3937
                     /locus_tag="VGR22_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127917.1"
                     /translation="MQYQQRPPTSFISQQLRGVINGFLAGLVTGVLIGWFFHGFVGLL
                     VRFGFVLILLIPLAFVVWYFFLRGGGRSPVGERGDGMQVFTWRSGEGPRRFTVDLPHT
                     PSEGEPERRHRRTQTDDETIDSEFRELKRRVDRDE"
     gene            3934..4710
                     /locus_tag="VGR22_04795"
     CDS             3934..4710
                     /locus_tag="VGR22_04795"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV2127918.1"
                     /translation="MIDPDLIASVAAITFGIGVILGFIGAGGAGTMLAVLTLVFGLSI
                     HEAVGTSLAAMVFVTISGTISHYRETNVELRAGVIVGLTGMIGAIAGAELGQVVSGDV
                     LQPLAGLALWFLALLMWIRTRLRHHPNELPPGEDPLGSPGRKIGTALLGLSGGTASAF
                     FGVGMAPFLQLGMLVILGLPLRITVGTTMLALIFISLSGSAALASHGQVSVPHLIGAT
                     VGMTLGSYAGAKLTGRAPLRVLRAAIVLTPFIAGTLLIVT"
     gene            4816..5127
                     /locus_tag="VGR22_04800"
     CDS             4816..5127
                     /locus_tag="VGR22_04800"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV2127919.1"
                     /translation="MTAAFTKDDVLEGLKNVYDPEIGINIVDLGLVYDADIAENGDVL
                     VTMTLTSLGCPLGPVIMQEVNNALKDLPDIGETDVKLVWTPPWSPENMSEEAKDELGI
                     W"
     gene            5240..6202
                     /locus_tag="VGR22_04805"
     CDS             5240..6202
                     /locus_tag="VGR22_04805"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV2127920.1"
                     /translation="MRQARLMRPQAIVVIFVVLVLLSRGEARFFQLARENDEADAQLA
                     LPAQQMPDQLPASATAPPEPTVVPTDTPMPTPTISPAPTPTPTPEPTPTPEPVPTERP
                     APPPSSDTEMAEVIIQPQTGRKEVAVTFDAGAERGYSLEILDLLDQYGAVGTFGVTGM
                     WAEANPDLVREMIERGHQVMNHGYSHDSFTGRSTGTGPMTDQQVRSEVLDTEEEVREA
                     TGGYEIASYFRFPYGDYNANVLRLLEQLGYDYTIWWGCDSKAWMGHTAEDIVEECGRE
                     KVAPGLIVLLHVDSRPDVEALPQLLEIYSTAGYDLVSVEQLMQP"
     gene            complement(6375..7226)
                     /locus_tag="VGR22_04810"
     CDS             complement(6375..7226)
                     /locus_tag="VGR22_04810"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2127921.1"
                     /translation="MPSTSTIRQQLTVPGPVPIHVDIHDGEGPALVLIHGISGSGDTW
                     LPVVPALGQHFTPVTIDLRGHGRSGKPAHGYLYDAYIGDFDRVLETLELEHPLILGHS
                     LGGLIALWWAARYPARAAALVIEDSPLHSGEDFRAAFDNWIQLNAMSVEELRAHYAEA
                     NPRWKAEVVQHRAEQMTSTAPAVFSELRDDSMANHGVDRIAEIEHIESSALLIHGDIE
                     TGGMVRPHDALDFERRLGNAVRARIPRGAHTLHLDVMREFLDLAIPFLRRHADAASHI
                     PGHVLGT"
     gene            7463..>7659
                     /locus_tag="VGR22_04815"
     CDS             7463..>7659
                     /locus_tag="VGR22_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127922.1"
                     /translation="MIRMNRRRFTWVATSGIASAALVGCGEAVDEDLTPTRIPDVAGA
                     PPTLAPQVEPFESEEVAEEDGA"
BASE COUNT         1314 a         2351 c         2629 g         1365 t
ORIGIN      
        1 cggcccgctt ttgtgcgctc gatcgaccat gagcgcgtgc cgagggtcgt agtgttgaac
       61 atcaggacgt gttccagttg tgcctggcga gatgccggag ccagcaggct gaccttcgtc
      121 gccgggcgac ccttcttcat cgtgatcgct gttgtccaga cgtcgagcgc cccctcggcg
      181 aagaggcgtt cggtgagcac ttcgtagaac tgcgggctca tgtcgtcgat attggtctcg
      241 atcaccacct cgccgtcggt ggcatcggcg tctgccatct cgccgatcca gacgcgcagc
      301 gcgttcggcc acggcagctc cttctggccg aacccgtagc cgatcacgct cggtgcgaag
      361 gaggggcggg tgaaggtcgc tagcgtggtg aggatcgtgg cacccgtcgg cgtcagcagc
      421 tcggcaggat gctcctgcat cgcgaggatc ggggtggcgg tcggggtgcc ggcatcggcg
      481 atcagctcca gcgtggccgg ggcgggcacc gggatggtgc cgtgggcgct gctggcgaat
      541 ccatggccga gctgtggcgg tccggaaaag acggcctcga tgccgagaag cgagagcccg
      601 actgctgtgc cgcagatgtc gacgatcgcg tcgatgccgc cgacctcatg gaagtggacc
      661 tggtcgaggc cggagtcgtg gattttcgct tccgccttcg cgagccgttc gaacatcgcc
      721 aacgcggccg cctgcacggg cgtggcaaat ccggcattca tgatcaggtc gcggatggtt
      781 gcccagtcgc ggtggtggtg aacctcgccc gtcacctcga cggtcacgcg ggttccgtgg
      841 atgccgtgtt gaccggcgcg ctcggcacgc actcggtagc cgccgagccg aagcttgccg
      901 agttcgctgg tcagctgttc caatgacagt ccggcgtcga ccagcgcgcc gagcgtcatg
      961 tcgccgctcg cgccagagaa ggggtcgaag taggcgattt tcacgcgatg ctcctcgtgc
     1021 ggtcttcgtt cttcaccaaa tgcccagatg cgctgggccc gacgcgcgga ggacgtccga
     1081 cctattgtga cccgatcctg gtgcggccgg ccattttgga cagagttccg tatttggggg
     1141 ttgatgcgag acatccctga gtgcggcaaa tagcttcaca gtgctattgt agctgcagcc
     1201 aaggactgga catattggca caacctgcta ctatcgtggc tgtacgtaca cacagcgtcc
     1261 gccatgggag tgggcgggcg caagggtagc gagtgtacct cgggacatgt cgcaggggga
     1321 gagcaatggc ctattacgtg tatacgttgc cgaaaccgtc gaaccgtacc aagccgtctg
     1381 tgggggtgag tgcgccgcgc aagccacctt cgacgaaaaa gtcgtagcta caagtccggc
     1441 gccgtggtca ccggtggccc gagcgaggcg ggccagtagg attgggcagg gaagcgggta
     1501 cgtcgcgacg cggacaggac cgtgtgcgac tggcggatga tctgacgcgc accggtctgg
     1561 ctgggggtgg acgcctacaa cggaaccatg ttcgatacat gatcagggac gtacggcgcc
     1621 ttgctggatt gagcagggcg ctgttcatga gcgcttcacg tcgtcgttcc cattgggtcc
     1681 aagtccgtcc ctccctgtac aatccggcca ttgcacgagc ggcgacgttc cgtggaagca
     1741 ggccagcggc gagccgctgc tggctggtcc gcagtgcaag gcgggcgatg gttcgatccg
     1801 ggaatcgagg acgatgatgg cagagcagta cgggggacac tcgatgatcg cgccgttgcg
     1861 gcgggtgctg gtgcgcaagc ctgcgccgcc ggcgggggag ggtgatgccg cgcggttcaa
     1921 ctaccccaac cagcccaacc acgacgtcgc ggtccgtgag cacgatgcgt ttgtgcggaa
     1981 gctgcaggaa ctgggcgtcg atgtgatcga ggcggaggca gctgccaccg gcgagctgga
     2041 cgctatcttc gtctacgacc cgtcgttcac aacggacggc ggcttgctgc tgatgcggcc
     2101 gggcaaggtg gcacgcgcca gcgaggtcga gatggctcgt gaagcggcat tcgcgctcga
     2161 tatcccgatc atcggcgaga tccaggagcc agggaccgtc gagggtggcg acatgctctg
     2221 gttggatcag aacacgctgg cgatcggcga ggggtaccgg accaatgcgg ctggcatcga
     2281 ccaggtccag atcttcctgc ggccggtggg cgtcgatgtc gtgcgtatgg cgctgcccta
     2341 ctggcacggt ccagacgagt gcctgcacct gatgtcgctg atcagcccgg tggccgagaa
     2401 gctggcggtg gtctacaagc cgctgatgtc ggtgccgttc gtccagtttc tcgaggacga
     2461 ggggtggtcg ttcgtcgaga tcccggatga ggagttcgcg acgcagggga gcaatgtgct
     2521 ggcgatcgag ccgggcaagg tgctgatgct caaggacaac ccggtcacga tcgaccgcct
     2581 gcgcaaggcg ggggtcgagg ttcacaccta cacaggtgac gagatctcgc acaaccgcgc
     2641 cgggggaccg acctgcctga cccgcccaat cctgcgccga acgctgacgg cgtaggcagc
     2701 gcctgaacat cacacctgtg ctggcagcac cagccgcatc gtcgcattgg ccgattccag
     2761 aggcgaacgc ggtggtgcca tgctgaaccg gcaaggcgcg ccagcgccgc agccgtgtcg
     2821 aggcatctct tgtgttttgg ccggcaggta tcgaaaggac cgaccgggtc aagctggagc
     2881 tggacgaagg tcctctatat ggtgtcacca acgatctcgc ggcccggctt taccagcacc
     2941 gtaatgtagg cgacaacacg tttgcagggc agtaccgcac acatcggctc gtgtacgtcg
     3001 aggagttacc gcggatcgac gacgcaatcg ctcgcgagaa gcagctcaaa ggctggaagc
     3061 gcgaccggaa ggtcgccctg atcgattcga tgaaccctgt gtggcgtgat cttgcggaag
     3121 actggggctg aaactcatct ccagctagcg agcctgtcaa cgaagcaaga gatccttcga
     3181 ctccggccgc tgctcaggat gacatcgttt cgttgatctt tagggaacgt gtgctcggtg
     3241 ccacagagag cccgcccacg gcggagcggg cctacacgtg gttacgaagt tggagtgtgg
     3301 cgagggcgca tgggagtcga acccaccggc gccgggatca ccgacgccca cgcggttttg
     3361 aagaccgggg aggccaccgg gccccttccg cccccatctc ggctgccgag acagcgtcgc
     3421 tcaagtatac ccatcgccgc cgggttcctc gtagtatggt gggagcgaca gcgccggcca
     3481 gcggcaccca ccacaccccg aatcgatcgc aggacacgcc tcgatgcagt accagcagcg
     3541 tccgccgaca tcattcatct cgcagcagct ccgcggtgtc atcaacggct ttctcgccgg
     3601 gctggtcacc ggcgtgctga tcggctggtt cttccacggc ttcgtcgggc tgctggtgcg
     3661 ttttgggttc gtgctgatcc tgctcatccc gctcgccttc gtcgtctggt acttcttcct
     3721 gcgtggcggc ggcagatccc cggttggcga gcggggtgac ggcatgcagg tcttcacgtg
     3781 gcgcagcggc gaggggccgc gccggttcac cgtcgacctg ccccacacgc cgtcagaggg
     3841 cgagccggag cggcgacatc gtcgcacgca gaccgacgac gagaccatcg attccgagtt
     3901 tcgggagctc aagcggcggg tggaccgcga cgagtgatcg atccggacct catcgcctct
     3961 gtcgcggcga tcaccttcgg gatcggtgtc atcctcggct tcatcggggc gggaggagcg
     4021 ggcaccatgc tcgcggtgct cacgctcgtc ttcgggctct cgatccacga ggcggtcggg
     4081 acgtcgctgg ccgctatggt ctttgtgacc atctccggca ctatctccca ctaccgggaa
     4141 accaacgtcg agctccgggc cggggtaatc gtgggcctga ccggcatgat cggtgcgatc
     4201 gccggggccg agctggggca ggtcgtctcc ggggacgtgc tgcagccgct ggccggactc
     4261 gcgctctggt ttctcgcgct gctgatgtgg attcgcacgc gcctccgcca ccacccgaat
     4321 gagttgccac cgggagagga cccgctcggc tcgccgggcc gcaagattgg cacggcgctg
     4381 ctcggtctca gcggtgggac cgcgtcggca ttcttcggcg tcgggatggc gccgttcctg
     4441 cagctcggga tgcttgtgat cctcggcttg ccgctgcgga tcacggtcgg cacgaccatg
     4501 ttggcgctca tcttcatcag tctcagcggc agcgccgcgc tggcgagcca cgggcaggta
     4561 tccgtgccgc acctgatcgg cgccaccgtc ggcatgacgc ttggctcgta cgccggagcc
     4621 aagttgacag gccgggcgcc attgagggtg ctgcgggcag cgatcgtgct cactccgttc
     4681 atcgcgggca cattgctgat cgtcacctag gtagaatagg gagagaaccg tacggacggg
     4741 gatggatgcc tgatggtggt ttgctgccac cgcaaccacc actgatccat tcccaaacac
     4801 aggaaagggt ggctcatgac tgctgccttc acgaaagacg acgtgttgga aggcctgaag
     4861 aacgtctatg acccggagat cggcatcaac attgtcgatc tcgggctcgt gtatgacgcc
     4921 gatattgccg agaacggcga cgtgctggtg accatgacgc tgacctcgct gggctgcccg
     4981 ctcggaccgg tgatcatgca ggaggtcaac aacgcgctca aggacctgcc ggacatcggc
     5041 gagaccgatg tgaagctggt ctggaccccg ccctggtcac cggagaacat gagcgaggag
     5101 gccaaggacg agctcggcat ctggtagctg tgcctgatgc ggggcgaggg cgtaccggta
     5161 cccggtcctg tcggatcttg atacccggtg tggggggacg aggacccggt atcggtccgg
     5221 tgtggagagg gaccggttgg tgcgacaagc gaggctgatg cgaccacagg cgatcgtggt
     5281 gatcttcgtg gtcctcgtgc tgctttcgcg cggggaggcg cgcttcttcc agcttgcccg
     5341 cgagaacgac gaggccgatg cccaactcgc cctgccggca cagcagatgc ctgaccaact
     5401 gccggcctcc gccaccgcgc cgcccgagcc taccgttgtc cccacagata cccccatgcc
     5461 gacaccgaca atctcaccag ccccgacgcc cacaccgacg cccgagccaa cgcccacccc
     5521 cgagccggtg ccgaccgagc gtcccgcacc gccgcccagc agcgacacgg agatggctga
     5581 ggtcatcatc caaccgcaga ccgggcgcaa ggaggtcgcc gtcaccttcg atgctggcgc
     5641 cgagcggggc tactcgcttg agatcctcga cctgcttgat cagtacgggg ccgtcggcac
     5701 cttcggtgtc acgggcatgt gggccgaggc gaatcccgat ctcgtacggg agatgatcga
     5761 gcgggggcac caggtgatga accatgggta ctcgcatgac tccttcaccg gtcggtccac
     5821 cggcaccggg ccgatgaccg atcagcaggt gcggtcggag gtgcttgaca ccgaggagga
     5881 ggttcgtgag gcgaccggcg ggtatgagat cgcgtcctat tttcgcttcc cctacggtga
     5941 ctacaacgcc aacgtgctca ggttgctcga gcagctgggc tacgactaca cgatctggtg
     6001 gggctgcgac tccaaggcgt ggatggggca caccgcagag gacatcgtcg aggagtgcgg
     6061 tcgcgagaag gtcgccccgg ggctgatcgt gttgctgcac gtcgattccc ggccggatgt
     6121 cgaggcgttg ccgcagctgc tggagatcta cagcactgct gggtatgacc tcgtgtctgt
     6181 cgagcagctc atgcagccgt agcgtgtccg gctttccggt ggttcctgga ggggaccaac
     6241 cttggggggc gacccgcgcg gcccgcttgg tcgcgccaca ggtacggccg gcacaaggcc
     6301 ggcgctacag ggcagattct ccggaaacca tccatccgcc acgccaacac cgttgctcag
     6361 ggaggcaacc ggcctcaagt gccaagcacg tgacccggga tgtgactcgc tgcgtccgcg
     6421 tggcggcgta ggaacgggat cgccaggtcg aggaactccc gcatgacgtc gagatggagg
     6481 gtgtgcgccc cgcgggggat gcgagcccgc accgcattgc cgagacgccg ctcgaagtcg
     6541 agggcgtcgt gcgggcgaac catgccgcct gtttcgatgt ctccgtggat cagaagcgcc
     6601 gagctctcga tgtgctcgat ctcggcgatg cggtcgacac catggttggc catcgaatca
     6661 tcccgcagct cactgaagac ggccggtgcc gttgacgtca tctgctcggc ccggtgctgc
     6721 accacctccg ccttccatcg gggattcgcc tccgcgtagt gcgcccggag ctcctcgact
     6781 gacatcgcgt tgagttggat ccagttgtcg aacgccgccc ggaagtcctc gccgctgtgg
     6841 agcgggctgt cctcgatcac gagggccgca gcacgtgccg ggtaccgggc ggcccaccag
     6901 agggcgatca gcccgccgag tgagtgcccg aggatgagcg ggtgctccaa ttcgagcgtc
     6961 tcgagcacgc ggtcgaagtc gccgatgtag gcgtcgtaga ggtagccgtg ggcgggtttt
     7021 ccggagcgcc cgtggccgcg aagatcgatc gtgacgggtg tgaagtgctg accgagagct
     7081 ggcaccactg gcagccaggt gtctccgctg ccgctgatgc cgtggatgag cacaagggcc
     7141 ggcccctcgc cgtcgtggat atcgacatga atcggtaccg ggcccggcac ggtcagctgc
     7201 tggcgaatgg tggaggtgga aggcatggcg actccggata cagatttagg tcgacggcgt
     7261 gcatcgtgtc aaacaattcc cggcacatgg aaatccggca acgaagtaaa ctataggaaa
     7321 atacgcggaa ggcggtgggt gcccatctcc gcaggcaggc ccggccgcct tttgagtgtg
     7381 ctgaatgaca gggagcgcag cacccattgc gccttgctcg atgcacgcga cacgtgacct
     7441 attagcgttt ggagaacatc atatgattcg gatgaatcgg cgtcggttta cgtgggttgc
     7501 caccagcggt atcgcaagcg cggcgctggt cgggtgcggt gaggcggtcg atgaggatct
     7561 gacaccgacg cggatccctg atgtcgcagg cgctccgccg acccttgcgc cgcaggtcga
     7621 accgttcgag tcggaggaag ttgccgagga ggatggcgc
//
LOCUS       DASYWL010000136        18581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_663087, whole genome shotgun sequence.
ACCESSION   DASYWL010000136 DASYWL010000000
VERSION     DASYWL010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 18581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18581
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663087"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VGR22_04820"
     CDS             complement(<1..313)
                     /locus_tag="VGR22_04820"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2127923.1"
                     /translation="MQGDATTGRTRHQASARYILPVLVFSALVMSIIATLGTPMVPTI
                     SRELDVSLETAQWLLTVTLLVGAVATPIGGRLADGSHRKLVVAGALAIVFLGSVIATT
                     IR"
     gene            527..1306
                     /locus_tag="VGR22_04825"
     CDS             527..1306
                     /locus_tag="VGR22_04825"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV2127924.1"
                     /translation="MGNRLQGKATIVTGGSGGIGLATAQLFHGEGASVAIVDLDRAAV
                     AEAAREVGDGALGIAADLSREEEAERAVRETVDRFGRLDVLVNAAAIRVYGPVTEATR
                     ESWERIVDVNLLGTAAMCKYAIPMMARNGGASVVNVSSSNASVGRGGMAQYDATKGAM
                     LSLTRALACDHAAEGIRVNALSPGPTLTPFHVRRRMMETGEPFEAAEAALRAVGARTL
                     LGRQAEPIEVARALLFLASDEASYITAIDLPVDGGLIGVRG"
     gene            complement(1388..1894)
                     /locus_tag="VGR22_04830"
     CDS             complement(1388..1894)
                     /locus_tag="VGR22_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127925.1"
                     /translation="MKRRLQIVVMAAVMVLGMVMAGTVGSGAVAQDATPAPPGVIEIA
                     PGVTADSVVFVPGRENPLLYRLNFEPGVTYPIQPAQSLELVYVEVGHLTVELDTPVTV
                     SQLGTEDTAGETYAAGMEFTVSVGEYFVLPPNASGVVRNEGLDTATVSVAGIPADISA
                     LPVATPEG"
     gene            complement(1995..2240)
                     /locus_tag="VGR22_04835"
     CDS             complement(1995..2240)
                     /locus_tag="VGR22_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127926.1"
                     /translation="MTAMSAMGASWEIPIGAPVISADGRRLGVVTEADAYELRVEDGI
                     FVHHAYALNLMDVAIVEGGALRLKLTAEEAVEQRGVS"
     gene            complement(2335..2562)
                     /locus_tag="VGR22_04840"
     CDS             complement(2335..2562)
                     /locus_tag="VGR22_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127927.1"
                     /translation="MPRDDKERVQKRERTTIDEARAEGIGEPEEGIEPAVPTYTDRGD
                     VSDLGVDEPVVPRDERGEKPEPAPEGQPDDG"
     gene            complement(2641..2883)
                     /locus_tag="VGR22_04845"
     CDS             complement(2641..2883)
                     /locus_tag="VGR22_04845"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HEV2127928.1"
                     /translation="MSRVTDPVCGRTIDTESAAARADYNGQTWWFCSERCHREFVTSP
                     QQHSDRLLPTTCPICGGEVGQDDLVCPHCGTNLVSG"
     gene            3141..3587
                     /locus_tag="VGR22_04850"
     CDS             3141..3587
                     /locus_tag="VGR22_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2127929.1"
                     /translation="MRANGQQAHVVVIDHTHEIGLLFEELLRSESYRATVLGDCPESV
                     DQIVALRPDVIVHDYALSHAEADRVSLRRLTTDPRTSHIPLVLCSAATDVDDIAARLG
                     GASVQVIAKPFTIDEVLGAIRSGARAHLPGITREVPPLQAPGASCH"
     gene            3668..3910
                     /locus_tag="VGR22_04855"
     CDS             3668..3910
                     /locus_tag="VGR22_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127930.1"
                     /translation="MEQARNTSKIELKRETMEQSMADLRLSLTAAWAWSQLLERRSQQ
                     GIPPTSEHVDRAAGAISTACEEMVRTLHDLESTYKT"
     gene            3925..4368
                     /locus_tag="VGR22_04860"
     CDS             3925..4368
                     /locus_tag="VGR22_04860"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEV2127931.1"
                     /translation="MPLTGEYAPSTSDWAREQAERFEASGGTEANTLRGMPIILLTTV
                     GAKSGKLRKTPLMRVEHEGEYAVVASLGGAPKHPVWYWNLKAHPHVELQDSPVKRDYV
                     AREVTGEEKAVWWERAVEAYPPYTEYQEKTDREIPVFVLTPIEEP"
     gene            complement(4486..6906)
                     /locus_tag="VGR22_04865"
     CDS             complement(4486..6906)
                     /locus_tag="VGR22_04865"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2127932.1"
                     /translation="MDGRSPESFGNVLRRYRILAGMSQEELAERAGLSVRGIGDLERG
                     QRRGPRLRTVQALADALGLDQVGRRELLAAARPELELHAPVQASDPPPSTPGRLALPM
                     LARMPVPSGSLIGRDADIETIAALLRGNHRRLVTLTGPGGVGKTQLAVAVARRVAGDF
                     ADGVAYVDLAPTRHPGMVGIQIARTLGLDDVGANRIIDMLREFLDERRLLLLLDNCEH
                     LLPAMPIAASLLATSPNLRVLATSRERLHLRDEREFAVDPLALPEYRSRTGQPLPPAE
                     LLGTAAIQLFVERAQEVDPRFVLTEENVSVVASICERLDGLPLAIELVAARLRHLSVA
                     AVRTRLEQHLPLHEDGPRDLPERQRTLSATIGWSYDLLSPAEQALFRRVSVLVGGFNV
                     PAAEAVALATPSGQPGDGAVPAVPRSAVFASLASLMDKNLLVQAIGDDGEARFAMLET
                     IREYAAERLATSGEEEAVRRAHAAHVLDFVERAAPELGGPDQQRWLNRLDLERDNLRG
                     ALAWAADRHDVESGLMLARRLARFWHIRGHAAEGRLWLERMLNAAEGIANDERAWPTY
                     YAGLLAWAQGDLAAAKGWFDESLGMFRDLDDRKGIATAVGMLGAVAEHEGELERAVEL
                     NQESLALYRELDSTPGIATLLDALGNIAVDQGDHDCATAYFDEALTLYRDLEDAGGIA
                     RVLSNIGALAFVRGDMDSAERASREGLVLHSKLGYKEGAAYALENLGRVAAAGRDAEQ
                     AARLWGAAEALREEAGTPLPPSAHESLRRDVAAVRQQLHSGVFRAAWEAGRSLSLEKV
                     VTDTLAES"
     gene            7125..8087
                     /locus_tag="VGR22_04870"
     CDS             7125..8087
                     /locus_tag="VGR22_04870"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV2127933.1"
                     /translation="MLHARWYLPARRSPRTGAVAALVAANVIWGTTFVATKPMLDRVP
                     PLTLASGRFAIAVLVLLLVLARAGRSPLLNRTTALMGFVGILIVYACQNLGLALTDAA
                     NGALIHGGIPVFTMLIAAPVLGERLSGGRLAGLAVSLIGVTMVVMRGSEEQLGLSAIG
                     DGLVLLSAIGLAAYLVLGRKAFAGESSIELVGGVAVFGLLFLLPVSGLELVTKGMERP
                     TTWDLLGLLYLGAVASGLAFMLWAHGLRHMEAGQAATFTNLNPLVGVIAAALVLNEAI
                     SWIQLAGGLLILGGVCLVARPIPVASFVEAVATPDSPEGELVSA"
     gene            complement(8636..8848)
                     /locus_tag="VGR22_04875"
     CDS             complement(8636..8848)
                     /locus_tag="VGR22_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127934.1"
                     /translation="MVGLVILSLLVLALIWRVGTLVGHRAGRRPAAAKFKSAARGQKR
                     NRLMVLSSQSIRVVYVNAHITRMARS"
     gene            9154..9558
                     /locus_tag="VGR22_04880"
     CDS             9154..9558
                     /locus_tag="VGR22_04880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2127935.1"
                     /translation="MESNEHRQTQRQHIFAVNGAIEFLEVLRELLQEEEYNVTTTNFV
                     PDTYEQIEALQPDLLIVDLAVGQQAGWDLLKRLADEARTNEIPVIVFSTNPKILEDVQ
                     KEPERFGGQRFLRKPIDLDEILDAIHELIGKA"
     gene            complement(9563..9739)
                     /locus_tag="VGR22_04885"
     CDS             complement(9563..9739)
                     /locus_tag="VGR22_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127936.1"
                     /translation="MVTRWMRRQGAGGDIDVMLERLAIIDETVMQLVAEVYALEEREP
                     PDDRQRRGDSSKSR"
     gene            complement(10626..12740)
                     /locus_tag="VGR22_04890"
     CDS             complement(10626..12740)
                     /locus_tag="VGR22_04890"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV2127937.1"
                     /translation="MPHRFPTSWRYSKWDGSEEMALDTDDLFDNISEDLLRDGDLDQA
                     LRRAFRWGMRGQDGSRQDGLRDLIDRLRQRRQDLLDRYDFDSVLGDLAERLQDIVDRE
                     HSTLDERLGQAQQGDGQQGAESGESGGQAGAQKGQQAGSQQGQPSGAGGQQGGGGEQD
                     ALRDFLERKRQRLDAIPNDAGSRIQQLQNYDFVDRQAGEDFEKLMEEMRQQITDSLFN
                     NLMGAAQNTDAGPAGDLKDFLRDLNEAFAQEAAGQPVDMDALNNKWSHMFGGRADSLD
                     EIRERMRQRMMAAQALMSLLSPEQRAELQAAMEQAIRDSGIEQELMELQEHLGPIPTP
                     DMNQASGPGQEELPLDMAMDVMERANEMQGVEEMLRAMVDPTDLDGIPDEMLRNVLSE
                     QDRDWLDQWSELRDRLIDEGYARRGRKGLELTPRAIRKIGEKALQDIFSSLKDLGAGE
                     HDIHQRGSAGESADTSAPWQFGDPFLLDLPRTVMNGLMREGPGTPVGLVQDDFEVIER
                     EAHTSTATALLIDMSRSMYYSGAWDAAKRSALALDTLIRGKYPRDRLELIGFSSVAHP
                     LQMTDLPSLNWNEYTYGTNLQHALELARQTLRSERGSNRQIIVITDGEPTAHIEEGRP
                     FFNYPPTWQTFEATLREVVRCTREGITINVFLLEHSPYMVRFVEDLMRINKGRVINAS
                     PTRLGSYVLRDFLQQRTLYRNG"
     gene            complement(12921..14330)
                     /locus_tag="VGR22_04895"
     CDS             complement(12921..14330)
                     /locus_tag="VGR22_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2127938.1"
                     /translation="MPTPPTISTLRELKDSGYRPKSVRDEVRTNLVAAMREGRNLFPG
                     IRGYDDTVLPELQNAILAGHDVIFLGERGQAKTRLARGLVQLLDEWLPIVAGSEINDD
                     PFDPVSHYGKTTVAEQGDDTRIDWVHRDRRFAEKLATPDTTVADLIGEVDPIKVAEGR
                     YLSNEEILSFGLIPRTNRGIFNLNELPDLAERIQVGLLNILEERDIQIRGFTVRLPLD
                     LMFVASANPEDYTNRGRIITPLKDRFGSQIRTHYPQALDYEIDITEQERDSFAAPGIE
                     VHYPSYMQEVVAEISHHARGSSDVSQRSGVSVRMTVANAEALLANAQRRAVMTGEKVA
                     VPRISDLPALRPSTMGKLELETFGEGRDDQVFDRLVGDAVKSVFLRSVDPSRLEMLLL
                     AFDNGLEVTVSDQANSFAYIPQVSAVEELKGVVDSLDGGKSPAEMASAVEFVLEGLHQ
                     MRKLSRKSSSDGDGVTYAR"
     gene            complement(14539..15321)
                     /locus_tag="VGR22_04900"
     CDS             complement(14539..15321)
                     /locus_tag="VGR22_04900"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEV2127939.1"
                     /translation="MSARIRVFGVLVLALVGGVFGASATTAQEATPATLPDQCTVVTD
                     GLVQPRDIAVGSDGSVYVVDGGDDAGRVSRIAPEGSITIVAEELAVSVADEAPSGLAV
                     DEIGQVHVVLANGAVAKVVEDGSQEVVLDGLPHPTGIAFDSDGALLLAAGGETGQLLR
                     CDLSVEFAAVTDVTISMQDIFFEPNEVAIPANTDVTLHVVNDGFVMHNLSIPAAGVTT
                     PILASGAAHDVVLNLPPGVYEITCDVRGHKGAGMVGILHVAG"
     gene            15491..16576
                     /locus_tag="VGR22_04905"
     CDS             15491..16576
                     /locus_tag="VGR22_04905"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEV2127940.1"
                     /translation="MQLVDAHLDLAMNALVLNRDLTKRAHDTRADEAGMTEKGRAAGT
                     VGLPDLRQAEIGLCLATVLARVNPEGTSPLDFRTHEIAHAHAQGELALYRELERQGRM
                     RMVTSREGLTRHVEMLSGLPGGPLPDDTPLGYVLLMEGADPIVAPDRVEGWWRDGLRV
                     VGLAHYGSSAYAFGTDSAGPLTEKGRALVAEMDRLGMILDASHLTDESFHDALDRFHG
                     PVLSSHSNCRALVPGDRQLDDEMIKRLAERGAVIGAVCDAWMLEPGWVRGESTPENVT
                     VANVVDHIDHVCQVTGTSRHAAIGSDLDGGYGTEQTPKDLDTIADLQQIPELLIARDY
                     SDDDIAGIMHGNWIRFLGDHLPSEPQA"
     gene            16573..17658
                     /locus_tag="VGR22_04910"
     CDS             16573..17658
                     /locus_tag="VGR22_04910"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEV2127941.1"
                     /translation="MTGQPRLRDLGITVGTLPTGARNAITDVPEVSVGHTTVIHEDGP
                     ARTGVTAIHPHAGSIFTSMAPAAIEALNGSGEMTGRSQVDEYGLLESPILITNTLSVG
                     TAHRACVEWIGQREPALGDTDFSIPVVAETYDGYLNDIGGQHVTIDHVFAALDSASDG
                     PVAEGNVGGGTGMRLFEFKGGIGTASRMVEIGGARYTVGVLIQGNYGSREHLLVDGVP
                     VGRELGVVAEQGVGKDGSVIVVIATDAPLTDRQLRRLCRRGMLGLARTGATANHTSGD
                     ILLAFSNAPENRVDRFAEDPLRTMSYINDSRINPFFSAAIEATEEAVLNALVAAETMT
                     GRDGHTVEAIPHNALMDVMRRYNWLGK"
     gene            complement(18056..>18581)
                     /locus_tag="VGR22_04915"
     CDS             complement(18056..>18581)
                     /locus_tag="VGR22_04915"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEV2127942.1"
                     /translation="TKVCDALEVPVVVKEVGFGISADVARQLHDAGVAAIDISGAGGT
                     SWSAVEHHRAGDDHYRRLSETFVDWGIPTAESLRMVRASLPNAVLFASGGMRTGLDAA
                     KAVALGADLVGFAGPMLKAAADSEAAAVAMLRILVEELRLAMFATGSRTLCDLRHVPL
                     LYAGGARLPLSDLR"
BASE COUNT         3163 a         6193 c         6182 g         3043 t
ORIGIN      
        1 agcggatggt tgtcgcgatg accgagccga ggaacacgat cgccagcgcg ccggcgacga
       61 ccagcttgcg atgggagccg tccgcgaggc ggccgccgat cggggtcgcg acagcgccca
      121 cgaggagcgt cactgtcagc aaccattgcg cggtctccag cgacacatcc agctcccggg
      181 agatcgtcgg caccatcggc gtcccgaggg tggcgatgat ggacatcacc agggccgaaa
      241 agacgagcac cggcaggatg taccgtgccg acgcctgatg ccgcgttcgt cccgtggtcg
      301 cgtccccctg catgccggtg gtgtcctctc gctgccgggt atcgccgtgt caccggagca
      361 gaccgttgcc ggacctccac caatggccga tcccaatcgt aaccgcgacc cggacgattg
      421 gaaacgccgc acgcaaccgc ttgactgcgc ccacggtggc gcgccataag gtgacgtccg
      481 gccgcaagcc ggcgcaacac aggcacgcac gacagaggag gcagcgatgg gcaaccggct
      541 tcaggggaag gcgacgatcg tcaccggcgg cagcggcggt atcgggctcg caaccgcaca
      601 gctcttccac ggggaggggg cgtcggtggc gatcgtcgac ctcgaccggg cggcggttgc
      661 cgaggcggcc cgcgaggtgg gggacggagc gctcggcatc gccgccgacc tctcccgtga
      721 agaggaggcc gagcgggcgg tgcgggagac ggtcgaccgg ttcgggcgac tcgacgtgct
      781 ggtgaacgcg gccgcgatcc gggtctacgg gccggtgacg gaggcgacgc gggagtcgtg
      841 ggagcgcatc gtcgacgtca acctgctcgg cacggcggcg atgtgcaagt acgcgatccc
      901 gatgatggcg cgcaacggcg gcgcgagcgt ggtcaacgtc tcctcctcca acgcgagcgt
      961 cgggcggggc ggcatggccc agtacgacgc gaccaagggg gcgatgcttt cgctgacgcg
     1021 ggcgctggcg tgcgaccacg cagcggaggg gatccgcgtc aacgcgctct cgccgggacc
     1081 gacgctgacg ccgttccacg tcaggcggcg gatgatggag accggggagc cattcgaggc
     1141 cgcggaggcg gcattgcggg cggtgggagc gagaaccctg ctcggacgcc aggcggagcc
     1201 gatcgaggtg gcccgagcgc tgctcttcct cgccagcgac gaggcctcct acatcaccgc
     1261 catcgacctc cccgtcgatg gcgggctgat cggcgtgcgc ggctagcgca tcgaggtcgc
     1321 cacggagaac ggacgaagcc gccggagagg atctcggagg ctcgcatcgt gcaatctttc
     1381 gctgtcgcta cccctcgggc gtggcgacgg ggagcgccga gatgtcggcg gggatgcccg
     1441 ccacggagac ggtcgccgtg tccagcccct cgttgcggac cacgccggaa gcgttgggcg
     1501 ggaggacgaa gtactcgccg acggagacgg tgaattccat accggcggca taggtctcac
     1561 cggcggtgtc ctccgtgccg agttgggaga ccgtcaccgg ggtgtccagc tccacggtca
     1621 ggtgcccgac ctccacgtac accaactcca gcgactgggc cggctggatg ggataggtca
     1681 cgccgggctc gaagttgagc cggtagagca gcggattctc tcggcccgga acgaagacga
     1741 cgctgtcagc ggtcacgccg ggggcgatct cgataacgcc ggggggagcc ggtgtggcgt
     1801 cctgcgccac ggcaccagag ccaacagtcc cggccatgac catgccaagc accatgacgg
     1861 cagccatcac gacgatctgg agacgacgct tcatcttttc ctctctcgcc catgaccgcc
     1921 aagcggcagc ccgtggcatc accctaccgg aatccccgag agattgaaag caggtgcgtc
     1981 acaagctccg aatctcagga aacgccgcgc tgctcgacgg cctcctcggc ggtgagcttg
     2041 agccggaggg cgccaccctc cactatcgcc acgtccatga ggttgagcgc gtaggcgtgg
     2101 tggacgaaaa taccatcctc gacgcggagc tcgtaggcat ccgcctcggt gacgaccccg
     2161 agccggcggc cgtcggcgga gatcaccggc gccccgatcg gaatctccca actcgcgccc
     2221 attgctgaca tcgctgtcac ttcggtccct ccttcagcgt tgccggttgc catagcaaca
     2281 cggcatcgcg gtccgttggg actgtcacta cgcatgtgac gtgccaaccg aggatcaccc
     2341 gtcgtccggc tggccctctg gagccggttc gggcttctcg ccgcgctcgt cccgggggac
     2401 caccggctcg tcgacgccca agtcggagac atcgccgcgg tcggtgtagg tcggcacggc
     2461 cggctcgatc ccctcctccg gctcaccgat gccctccgcc cgggcctcgt cgatcgtggt
     2521 gcgctcgcgc ttctgcacgc gctccttgtc gtccctcggc actgttccct ccttctctac
     2581 cctctggcgt cgcgctcagg ggcatcgacg cccagagcgt cgcccctacc caccctagct
     2641 ctagcccgat acgagattgg tgccgcagtg gggacagacc agatcgtcct gaccgacctc
     2701 gccgccgcat atagggcagg tggtcggcag gaggcgatcg ctgtgctgtt gcgggctggt
     2761 cacaaactcg cggtgacagc gctccgagca gaaccaccag gtctgtccgt tgtagtcggc
     2821 acgggcggca gcgctctccg tgtcgatcgt ccggccgcag acgggatccg tcaccctgct
     2881 catcgcttcc tccatcacgc tggcgcgacg cgcgcatcac ttcatcggcg aaggatagca
     2941 gcccctgaca ccgacccagc aggggctgga caggcatcgt atactggata tatcgagctt
     3001 gtacggacat cggacgtcca ttggagctcg tctccacact cccgctgtgg gtttcccgtg
     3061 tagtcgcctt gtttcgggag cgtctgcatc ccgtcggtcc attgtggaac ttgaccgtca
     3121 cgccacctga aggagcggcc atgcgcgcca acggccagca agcccacgtt gtcgtcatcg
     3181 atcacaccca cgagatcgga ctcctgttcg aggagctgct gcggtcggaa tcataccggg
     3241 cgaccgtcct gggcgattgt cccgaatcgg tcgaccagat cgtcgcgctg cggccggacg
     3301 tgatcgtcca cgactacgcg ctgtcgcatg ccgaggccga ccgggtctcg ctccgaaggt
     3361 tgaccaccga cccgcgcacc agccacatcc cactggtgct ctgcagcgcg gcgacggacg
     3421 tcgacgacat cgccgctcgc ctcggcgggg cgagcgtgca ggtcatcgcc aaaccgttca
     3481 cgatcgacga ggtcctcggc gccatccgga gcggcgcccg tgcccatctc cccggcatca
     3541 cgcgcgaggt gccgccgctg caggcgccgg gggcctcctg ccactgacca gccaagccat
     3601 ggcactgttt ctgcaacctt cctgttacgg cgagcgttga ccgccagcac gtacagggac
     3661 agcagccatg gagcaagcca ggaacacgtc gaaaatcgag ctgaagcgcg agacgatgga
     3721 gcagtcgatg gccgacctgc gcctctcgct gaccgccgcc tgggcgtggt cgcagctgct
     3781 ggagcgtcgc tcgcagcagg gcatcccccc gacaagcgag cacgtcgacc gggcggccgg
     3841 ggcaatctcg acggcctgcg aggaaatggt ccgcacgctg cacgacctcg aatccaccta
     3901 caagacgtga ggagagactg accgatgccc ctgacgggag agtacgcacc gagcacgagc
     3961 gactgggccc gcgagcaggc cgagcgcttc gaagcgagcg gcggcaccga ggcgaatacg
     4021 ctgcgcggga tgccgatcat cctgctgacg acggtgggcg cgaagagcgg caagctgcgc
     4081 aagacgccgc tgatgcgggt cgagcacgag ggcgagtatg ccgtggtggc gtcgctgggc
     4141 ggggcgccga agcatccggt gtggtactgg aacctcaagg cgcacccgca cgtcgagctg
     4201 caggacagcc cggtgaagcg ggactacgtg gcgcgggagg tgaccgggga ggagaaggcg
     4261 gtctggtggg agcgggcggt ggaagcgtat ccgccctaca cggagtacca ggagaagacg
     4321 gaccgcgaga tcccggtgtt cgtcctcacg ccgatcgagg agccctaagc ctcggaggca
     4381 cgctggagcg ccaggttcca ggctgccaac aagcgacccg tggtcgcgtg gagggaccca
     4441 aacgggacac ctgatcttgc gcgtggacgt gtccacatgt ccaacctacg actctgcaag
     4501 tgtatcggtc acaaccttct caagggaaag agatcgtccc gcttcccaag ccgctctgaa
     4561 cacaccgctg tgcaattgct gacggacagc cgcgacgtcc cgccggaggc tttcgtgagc
     4621 cgacggaggg agaggtgtcc ccgcctcctc gcgcagtgcc tcggcagcac cccatagccg
     4681 cgccgcctgc tccgcatccc gcccggccgc ggccacccgc cccaggtttt ccagcgcgta
     4741 cgcagccccc tccttgtacc ccaacttcga gtgaagcacg aggccttcgc ggctggcgcg
     4801 ttctgccgaa tccatatcac cgcgcacgaa cgccaaggcg ccgatgttgc ttagcacacg
     4861 agcgatgccg ccggcatcct cgagatcgcg gtagagcgtg agcgcctcgt cgaagtacgc
     4921 cgtcgcgcag tcatggtccc cctggtcgac ggcgatattc ccgagcgcat ccagcaacgt
     4981 ggcaatgcca ggcgtgctgt ccaactctcg gtagagcgcg aggctctctt gattgagctc
     5041 gactgctcgt tccagctcac cctcatgctc tgcgacagct cccagcatcc cgaccgcagt
     5101 ggcaatcccc ttcctgtcat cgaggtcgcg gaacatcccc aggctctcgt cgaaccaacc
     5161 cttggcagcg gccagatcgc cctgcgccca ggccagcagc ccggcatagt aggtaggcca
     5221 agcacgctcg tcattcgcga tcccctcagc ggcattgagc atgcgctcga gccacaggcg
     5281 tccctccgct gcgtgccctc tgatgtgcca gaaccgcgcg aggcgccgcg ccagcatgag
     5341 tcccgattcc acatcgtgac gatcggcggc ccacgcgagc gccccgcgaa ggttgtcacg
     5401 ctcgagatcg agccggttca accaccgttg ctgatcgggg ccgcccaact ccggcgccgc
     5461 ccgctccacg aaatcgagca catgcgccgc gtgcgctcgc cgaactgctt cctcctcacc
     5521 actcgtcgcc aagcgttcgg cggcgtactc tcgtatggtc tccagcatcg caaaccgggc
     5581 ttcgccgtcg tcgccgatgg cctgcacgag caggttcttg tccatcaggg atgcgagact
     5641 cgcgaagacg gcggagcggg gaactgccgg gaccgcacca tcgcccggtt gtccactcgg
     5701 tgtcgctagg gcgaccgcct cggcggcagg cacgttgaat ccaccgacaa ggaccgatac
     5761 ccgccgaaag agcgcctgct ccgccggcga gagcaggtcg taactccagc cgatagttgc
     5821 actcagcgtg cgctggcgct ccggcagatc gcgaggcccg tcctcgtgga gcggcaggtg
     5881 ctgctccagc ctggttctca cggctgcgac cgagaggtgc cgcagccgtg ccgcgacgag
     5941 ctcgatggcc agcggcaggc catccaggcg ctcgcagatg gaagcgacca cgctgacatt
     6001 ctcctcagtc aggacgaacc taggatcgac ctcctgcgcc cgctcgacga agagctggat
     6061 cgcggctgtg ccaaggagct ccgcaggggg cagcggctgt ccggttcgag agcgatactc
     6121 gggcaacgcc agagggtcga cagcgaactc tcgctcgtcg cgcagatgca ggcgttcacg
     6181 gctcgtcgcg agcacccgaa ggttgggaga ggttgccaga agggaggcag cgatcggcat
     6241 cgctggcaag aggtgctcgc agttgtcgag gagcagcaga agtcgtcgct cgtcaaggaa
     6301 ctcccgcagc atgtcgatga tccgattcgc acccacatcg tcgagaccca gggtccgggc
     6361 gatctgtatg ccaaccatgc caggatgtcg ggtgggggcg agatcgacat acgcgacccc
     6421 gtcagcgaag tctccggcca cccgtctcgc gacggcgaca gcaagctgcg tcttgcccac
     6481 acctccgggt cccgtcagtg tcaccaggcg ccgatggttg cctcgcagca gggcagcgat
     6541 cgtctcgata tcggcgtccc ggccgatcag gctcccagag gggacaggca tccgcgcaag
     6601 cattggcagc gccaagcgac caggggtcga tggtggaggg tccgaggctt gcaccggcgc
     6661 gtggagctcg agctcaggcc gagctgccgc cagcagctcg cgacgaccga cctggtccag
     6721 tccgagggca tcagccagcg cctggaccgt cctgaggcgg gggcccctcc gctgaccgcg
     6781 ttccaggtca ccgatcccgc ggacactcag ccccgcccgt tcggccagct cctcctgact
     6841 catccctgcc aggatccgat agcgtcggag cacgttgccg aaggactcgg ggctcctgcc
     6901 gtccatcccg ttcctttcag gtttcgaacg cggcacctac cgatggtgtg ggaccctgag
     6961 aacttcatca tatcaccgga gcggcatcag tctccatatc gtcattaccg tgtcgaaact
     7021 gccgctgatc tgccagcgct gcgtctggtg ggcgaacgac ccggccggta ggctgggtgg
     7081 acaggcccct tcaggagtta ggctgacgaa ctgtgaggcg acgcatgctg catgcacggt
     7141 ggtacctacc ggcgagacgc tccccccgca cgggtgccgt tgccgccctg gtggcggcga
     7201 atgtcatctg gggaacaaca tttgtggcaa ccaagccaat gctcgaccgg gtaccgccgc
     7261 tcaccctggc aagtggccgg ttcgccatcg ccgtgctcgt cctccttctc gtgctggcga
     7321 gggccggccg gagtcccctg ctcaaccgca ccacggcgct gatgggattc gtcggaatcc
     7381 tgatcgtgta cgcctgccag aacctcgggc tggccctcac cgatgccgcc aacggcgcgc
     7441 tgatccacgg cgggatcccc gtcttcacca tgctcatcgc tgccccggtg cttggggagc
     7501 ggctcagcgg cgggcgactg gccggacttg ccgtctcgct catcggggtc acgatggtcg
     7561 tgatgcgcgg ttccgaggaa cagctcggcc tgtcagcgat cggtgatggc ctcgtgctgc
     7621 tcagtgcgat cggactggcg gcctacctcg tgctcggacg gaaggccttt gccggcgaga
     7681 gctcgatcga gcttgtcggt ggtgtggcgg tgttcgggct gctcttcctg ctgcccgtga
     7741 gcgggctgga actggtcacc aagggcatgg agcgcccgac gacctgggat cttctgggcc
     7801 tgctctatct cggcgccgtc gcctccgggt tggcgttcat gctttgggcg cacggactac
     7861 ggcacatgga ggcaggccag gcggcgacgt tcacgaacct gaacccgctg gtcggggtga
     7921 tcgccgcggc cctcgtgctc aacgaagcga tctcatggat ccagctcgcc ggtggcctgc
     7981 tcatccttgg aggcgtctgc ctggtagccc gcccgatccc cgttgccagc ttcgtcgaag
     8041 cggtagccac gccggactcg ccggagggtg agctcgtatc ggcctagacc ggctccctga
     8101 atgggttggc gaagccagca gagaccaatg ctggctgaaa acgaacgata tcggcagtgt
     8161 ggcagcctcg agaggggctg cgcatctcac gcacgacttc tctctcgatc gtccactcac
     8221 ccgaaagcac tcggacccct tccactacac caaggcatta atgggaaatc aagatcgttt
     8281 tgtcgcgctg atggaacggg acctcgatat gcttcaggga ctaatattcg ccacgatgga
     8341 gcatattgaa tccgattcgc gataccagac cgggacctat gccaaggcgt tgatgaagga
     8401 gctgacgctg atcaacgggt tccgtgaact gagttttgtc gatgatgact tactccatgc
     8461 tgttgtcgca gaggcagcga ccagaatacc agtagtcaag caaagggtca gtggaagatc
     8521 ctcgaatcgg gtcaggcctg cagcgacata gatgcgacgt tgcagctgtc ggctccggag
     8581 gtagtgatcg acactaccgc gaggcggtat gctgaggcgc actgtcgatc accgttcatg
     8641 aacgtgccat ccgggtgatg tgcgcgttga cgtagacgac acggatcgac tggctggaga
     8701 ggaccattag ccggttgcgc ttctgccccc gagccgcaga cttgaacttc gctgctgccg
     8761 gtcttctccc ggcgcgatgg cccacgagcg tgccgactcg ccagatgagc gccaacacga
     8821 gcagcgacag gatgacaagt cccaccatcg gcaggcaccc gcagccgggc aggcacccct
     8881 tgcggcgggg cccctcctcg acctcgatga ccgccctgta acgtatgcgg cgtctcgtgc
     8941 tcgtgatctc gataccctcc aggcatgcag acgttgtcgg gccgttgcgg ccatcatagc
     9001 agggggcgac ggctcaccgg gatcgtctcg atcggtcccc atccgaaaca tcgcgcagca
     9061 ccgctgattt tgctaccatc gcggcatgtc atcatcacag gagcgagatc ggcgggcgac
     9121 agggtgtgtg cgccgagggg acgagagcag ccgatggaat cgaacgagca ccgccagacg
     9181 cagcgccagc atattttcgc cgtgaatggc gccatcgagt tcctcgaggt gctgcgggag
     9241 ctgctgcagg aggaggagta caacgtcacc acgacgaact tcgtccccga cacctacgaa
     9301 cagatcgagg ccctgcagcc ggacctgctc atcgttgacc tggccgttgg ccagcaggcg
     9361 ggctgggacc tgctgaagcg cctggcagac gaagcgcgga ccaacgaaat ccccgtcatc
     9421 gtcttctcca ccaatccgaa gattctggaa gacgtgcaga aagagcccga gcgcttcggg
     9481 ggacagcggt tcctgcgcaa acccatcgac ctcgatgaga tcctggacgc gatccacgag
     9541 ctgatcggca aggcatagga cgctaccgcg acttcgacga gtctcctcga cgctgccgat
     9601 cgtccggcgg ttccctctcc tcgagcgcat acacctcggc gacgagctgc atcacggtct
     9661 cgtcgataat cgccagccgt tcgagcatca cgtcgatatc cccgccggca ccttggcgtc
     9721 gcatccagcg cgtcaccatc tgttcctagg cccggagggc agacagctgc gtctgtaacc
     9781 ggtggactgt gtcgggcgag agactatcgc gctggtgtgg atccgaattg gacatggctg
     9841 gctccgtcgc gcgtgccacc gcccggatgc gagcggttgg cgtatgggga acgcgtacac
     9901 gaggcgtatc atgcccggca tcctcgatga ttccagtaca acgttgggga tgatccgaag
     9961 gtgtggcgct gtcgacggtg cgtgcggaga tcggggcagg tggtcgtgag catcgcccag
    10021 tggtgacggg gaccgcgggg taccggagtc gaggttgccg ccgtggcagg gccgttccgg
    10081 cctttcggcg gctagggaac aggactgtac gaaacccctt gacaggtgta cgtacacctg
    10141 ctatagtggg agcagatgcc agccccgtcg acccggtacc aagtcggacg ccccccgttc
    10201 gaaaatggcg tggagctgga ggacgcgccg gcgagatcgc aatccgcgat cccggcgcgt
    10261 catcgtgttc gccatcgaga ttcggcagga cacactgcaa ggacccggcc aggcatcaga
    10321 gagacgacac gctgccgatg attgaggccg cggcacgtag ggcggccggg ccgaccactg
    10381 cctccgggag gtcgacccct acgatttccg aaaagttctc tgcgagcccg gacgttgagt
    10441 tccggcgcgc aagagattgc tcgacttcgc cctcgcgatg ctcgcgcttc gcccgaaacg
    10501 acagggtgcg ggatgggtga ggttgcgaga tggggtcagg gaatcaatgc ctggaggaag
    10561 cgagcgtgcc gggcaccggg cggacaccct gtcggggccc gcccgtgcgg acgtacgtgt
    10621 tgcctctatc cattgcggta gagggtgcgc tgctggagga aatcacggag gacgtaggag
    10681 cccagccgcg tcggcgaagc attgatcacc cgccccttgt tgatgcgcat caggtcctcg
    10741 acaaagcgga ccatgtacgg cgagtgctcg agcaggaaga cgttgatggt gatgccctcc
    10801 cgcgtgcagc gcacgacctc acgcagcgtc gcctcgaagg tctgccacgt cggcgggtag
    10861 ttgaagaacg gccgcccctc ctcgatgtgc gcggtcggct cgccgtcggt gatgacgatg
    10921 atctgccggt tgcttccccg ctcgctccgc agcgtctggc gtgccagctc cagcgcatgc
    10981 tgcaggttgg tgccgtaggt gtactcgttc cagttgagcg agggcagatc ggtcatttgc
    11041 agcgggtgcg ccaccgacga gaagccgatc agctcgagcc ggtcacgcgg gtacttaccg
    11101 cggatcaggg tgtcgagcgc gagcgccgat cgcttggcgg cgtcccacgc gccgctgtag
    11161 tacatcgaac ggctcatgtc gatcagcagc gcggtcgccg tgctggtatg agcctcccgc
    11221 tcgatcacct cgaagtcgtc ctgtacgagc ccgaccggcg tgccgggacc ctcccgcatg
    11281 aggccgttca tcacggtgcg cggcaggtcg agcaggaagg gatcgccgaa ctgccacggt
    11341 gcactggtgt cggcgctctc gccggcgcta ccgcgctgat ggatgtcgtg ctccccggcg
    11401 ccgaggtcct tcagcgagga gaagatgtcc tgcagggcct tctcgccgat cttgcgaatc
    11461 gcgcgcggcg tcagctccag gcctttgcgt ccgcgccggg cgtacccctc gtcgatcagg
    11521 cggtcgcgga gctcgctcca ctggtcgagc cagtcgcggt cctgctcact gagcacattg
    11581 cgaagcatct cgtcggggat gccgtcgagg tcggtggggt cgaccatcgc ccgcagcatc
    11641 tcctcgacgc cctgcatctc gttggcgcgc tccatgacgt ccatcgccat gtcgaggggc
    11701 agttcctcct gccccggtcc gctcgcctgg ttcatgtccg gtgtcgggat cggcccgagg
    11761 tgctcctgga gctccatgag ctcctgctcg atgccggaat cgcggatcgc ctgctccatc
    11821 gcggcttgga gctcggcgcg ctgctcgggc gagagcaggc tcatgagcgc ctgggcggcc
    11881 atcatgcgct ggcgcatccg ctcgcgaatc tcgtcgaggc tgtcggcacg cccgccgaac
    11941 atgtggctcc atttgttgtt gagcgcatcc atgtcgaccg gctgcccggc ggcttcctgg
    12001 gcgaaggcct cgttgagatc gcgcaggaag tccttgaggt cgcccgcagg accagcgtcg
    12061 gtgttctggg cggcacccat gaggttgttg aagagcgaat cggtgatctg ctggcgcatc
    12121 tcctccatca gcttctcgaa gtcctcgccg gcctggcggt cgacgaagtc gtaattctgg
    12181 agctgctgga tacgcgagcc ggcgtcgttc gggatggcgt cgagccgctg cctcttgcgc
    12241 tcgaggaagt cacgcagggc gtcttgctcg cctcccccgc cctgctgccc gccggcaccg
    12301 gacggctggc cttgctgtga gccagcctgc tgccccttct gcgcgcccgc ctggccgccc
    12361 gactcaccgc tttcggcgcc ctgctgcccg tcgccctgct gcgcctgacc gagccgctcg
    12421 tcgagggtgc tgtgctcccg gtcgacgatg tcctgcagcc gttccgcgag gtcaccgagg
    12481 acggagtcga agtcgtagcg gtcgagcagg tcctggcgcc gctggcggag gcggtcgatc
    12541 aggtcgcgca ggccgtcctg gcggctgccg tcctgcccgc gcatgcccca ccggaaggcg
    12601 cgccggagag cttggtcgag gtcaccgtcg cgcaacaagt cctcggagat gttgtcgaag
    12661 agatcgtcgg tatcgagcgc catctcctcg gagccatccc atttggagta gcgccaggag
    12721 gttgggaaac gatgcggcat ggtggcctcc ggatggtcgg ggttatgggg ttatggagca
    12781 gggcggacat ggagcccgcc cgtgcgggtc gactccatgt cgaccctgtt gtcgagtccg
    12841 atgcgcccgc tgtgcgactg ccaggcggcg ggccgaccac tccttgcgaa ggtcggtccc
    12901 tacggaatcg ccaggtccga tcagcgggcg taggtgacgc cgtcgccgtc ggagctcgac
    12961 ttgcggctca gcttgcgcat ctggtgcagc ccctccagca cgaactcgac cgcagaggcc
    13021 atctccgctg gcgacttgcc accgtcgagc gaatcgacca cccccttgag ctcctcgacg
    13081 gcgctcacct gagggatgta ggcgaaggag ttggcctgat cggagacggt gacctccagc
    13141 ccgttgtcga aggcgagcag cagcatctcc agccgggagg ggtcgacgct gcgcaggaag
    13201 acactcttca ccgcatcgcc caccagccgg tcgaagacct ggtcgtcacg cccctcgccg
    13261 aaggtctcca gctccagctt gcccatggtt gaggggcgca gcgccgggag gtcggagatg
    13321 cgcggcaccg cgaccttctc gccggtcatc accgcgcgcc gctgggcgtt ggccagcagc
    13381 gcctcggcgt tggcgacggt catgcgcacg gagacgccgc tccgctggga gacatcggac
    13441 gacccgcgcg cgtggtggga gatctcggcg accacctcct gcatgtagct cggatagtgc
    13501 acctcgatgc ccggcgcggc gaagctgtcg cgctcctgct cggtgatgtc aatctcgtag
    13561 tcgagcgcct gcgggtagtg ggtgcggatc tgcgagccga atcggtcctt gagcggcgtg
    13621 atgatgcgcc cgcggttggt gtagtcctcg gggttggccg aggcgacgaa catgaggtca
    13681 aggggcagcc gcaccgtgaa gccgcggatc tggatgtcgc gctcctcgag gatgttgagc
    13741 aggccgacct ggatgcgctc ggccagatcg ggcagctcat tcaggttgaa gatgccgcgg
    13801 ttggtgcgcg ggatgaggcc gaacgagagg atctcctcgt tgctgaggta gcgcccctcg
    13861 gcgaccttga tggggtcgac ctcgccgatc aggtcggcga ccgtggtgtc gggcgtggcg
    13921 agcttctctg cgaagcggcg atcgcggtgc acccagtcga tacgggtgtc gtcgccctgt
    13981 tcggcgaccg tcgtcttgcc atagtgggag acggggtcga aggggtcatc gttgatctcg
    14041 gagccggcga cgatcggcag ccactcgtcg aggagctgca cgaggccgcg tgcgaggcgg
    14101 gtcttcgcct ggccgcgctc gccgaggaag atgacatcgt gcccggcgag gatcgcgttc
    14161 tggagctcgg gcagcaccgt gtcgtcgtag ccacggatgc cggggaagag gttgcgcccc
    14221 tcccgcatgg cggcgaccag gttggtgcgc acctcgtcgc gcaccgattt cgggcggtag
    14281 ccggagtcct tcagctcgcg cagggtggag atggtcggag gcgtcggcat ggatgctgtc
    14341 ctctgcgatc gggcgggtgt cccgctgcat gtccggatcg tcggtgcccc gagtgtatag
    14401 gcggggggca tcattgtcac cggcgagtca accggtgtcg gtagcgcctg tccggaaacg
    14461 aaacgccgga aacggaagaa ccgcccggtc gagcgcacca ccgggcggcg tgtcacgtgg
    14521 ctctgttggt taatgctctc agccggcgac gtggagaatg ccgaccatgc cggcgccctt
    14581 gtgacctcgc acgtcgcagg tgatctcgta aacgccgggg ggcaggttca gcacgacatc
    14641 gtgagccgca cccgacgcga ggatcggcgt ggtgacaccg gctgcgggga tcgacaggtt
    14701 gtgcatcacg aaaccgtcgt tgacgacgtg cagcgtgacg tcggtgtttg ccgggatggc
    14761 cacctcgttc ggctcgaaga agatgtcctg catcgagatg gtcacgtcag tgaccgccgc
    14821 gaactcgacg gagaggtcgc accgcagcag ctgacccgtc tcgccaccag ccgctagcaa
    14881 cagtgcgcca tcactgtcga aggcgatgcc ggtcgggtgc ggcagcccgt cgagcacaac
    14941 ctcctgcgat ccatcctcga ccaccttcgc cacggcgccg ttcgcgagaa cgacgtgcac
    15001 ctgcccaatc tcgtcaacag cgagcccgga cggggcttcg tcagcaaccg agaccgccaa
    15061 ctcctccgcc acaatcgtga tggagccttc cggcgcgatt ctggagacac ggccggcatc
    15121 atcgccgccg tcgacgacgt agacgctgcc atcgctccct acggcaatgt cccgcggctg
    15181 cacgagccca tcagtgacga ccgtgcactg atcgggcagc gtggccggtg tggcctcctg
    15241 tgcagtggtg gccgaagcgc cgaacactcc acccaccaag gcaagaacaa ggactccgaa
    15301 aacgcgaatc cgggcgctca tgtgtacctc acttggaaca tgccttcagt cagacggacc
    15361 cattgctgat cgtaggcgat ggcggtccgc ttggcaacag gaagctcctg tgcattgggg
    15421 cttgccgccc taccgattct gactgacgat ggcgctgtgc gactcaccga atccccgaac
    15481 ggaggctctc atgcaactcg tcgatgccca tctcgacctc gccatgaacg cgctggtgct
    15541 caaccgcgat ttgacgaagc gcgcccacga cacccgcgcc gacgaggcgg gcatgacgga
    15601 gaagggccgc gccgccggca cggtcggcct gccggacctg cgccaggcgg agatcgggct
    15661 ctgcctggcg accgtcctgg cgcgggtgaa tccggagggc acatcgccgc tcgatttccg
    15721 cacccacgag atcgcccacg cccacgcgca gggtgagctg gcgctctacc gcgagctcga
    15781 gcggcagggc cggatgcgga tggtcaccag ccgcgaggga ctgacgcgcc acgtcgagat
    15841 gctgagcggt ctgcccggtg gtccgctgcc ggacgacacg ccgctcggct acgtgctgct
    15901 gatggagggc gccgacccaa tcgtcgcacc ggatcgcgtc gaagggtggt ggcgtgacgg
    15961 gctgcgcgtc gtcggcctcg cccactacgg ttcgagcgcc tacgccttcg gcacggacag
    16021 cgccggtccg ctcaccgaga agggccgggc gctggtcgcg gagatggatc gcctggggat
    16081 gatcctcgac gcatcgcacc tgaccgatga gagcttccat gacgcgctcg accgcttcca
    16141 cgggcccgta ctctccagcc actccaactg ccgtgcgctc gtgcccggcg accgccagct
    16201 ggacgacgag atgatcaagc ggctggccga gcgcggtgcg gtgatcggcg ccgtctgcga
    16261 cgcgtggatg ctcgagcccg gctgggtgcg gggcgagtcg acgccggaga acgtcaccgt
    16321 cgccaacgtg gttgaccaca tcgaccacgt ctgtcaggtc accggcacct cacgccacgc
    16381 cgccatcggc agcgacctcg acggaggcta cggcaccgag cagacgccca aggacctgga
    16441 caccatcgcc gacctccagc agatcccgga gctgctcatc gctcgcgact acagcgatga
    16501 cgacatcgcc ggcatcatgc acggcaactg gatccgcttc ctcggcgacc atctgccgtc
    16561 ggagccacag gcgtgacggg ccagccgcgg ctccgcgacc tcggcatcac cgtcggcacg
    16621 ctgccaaccg gtgcacgcaa cgccatcacc gacgtgccgg aggtcagtgt cggccacacc
    16681 acggtcatcc acgaagacgg gcctgctcgc accggtgtca ccgcgatcca cccacacgcc
    16741 ggctcgatct tcacgtccat ggctcccgcg gcgatagagg ccctgaacgg ctccggggaa
    16801 atgacgggcc ggtcgcaggt cgacgagtac gggctgctgg agtcgccgat cctcatcacc
    16861 aacacgctct ccgtgggaac ggcgcaccgg gcgtgcgtgg agtggatcgg gcagcgcgag
    16921 ccggcgctcg gcgacaccga cttctcgatc ccagtcgtcg ccgaaaccta cgacggctac
    16981 ctgaatgaca tcggtggtca gcacgtgacg atcgaccacg tgttcgccgc gctcgactcg
    17041 gcgtccgacg ggccggtcgc cgagggaaac gtcggcggtg gcaccgggat gcggctcttc
    17101 gagttcaagg gcggtatcgg gacggcgtca cgcatggtcg agatcggcgg cgcacgatac
    17161 acggtcggcg tgctgatcca gggcaactac gggtcacgcg agcacctgct ggtggacggc
    17221 gtgccggttg gccgcgagct cggtgtcgtt gccgagcagg gtgtcgggaa ggacggatcc
    17281 gtgatcgtgg tgatcgccac cgacgcaccc ctcaccgacc gccagctgcg acgcctctgc
    17341 cggcgcggca tgctcgggct cgcccgcact ggcgcgacgg cgaaccacac ctccggcgat
    17401 atcctgctgg ccttctcgaa cgccccggaa aaccgcgtcg atcgcttcgc cgaggatccg
    17461 ctccgcacca tgtcctacat caacgacagc cggatcaatc ccttcttctc ggccgcgatt
    17521 gaggcgaccg aggaggcggt gctcaacgcg ctcgttgccg cggagacgat gacgggtcga
    17581 gacgggcaca ccgtcgaagc gattccccac aatgccctga tggacgtcat gcgccgctac
    17641 aactggctgg gaaaatgaga cggcgacggc accgcaggat gagaacagcg gggaggagcg
    17701 acatgcctga tacgaaatcc gcctgagagc ttgcgccaat aggcaggcac caaggacgac
    17761 gagggcacag ggtgctccac cgtccgggaa ggtcttcaag gctttccaat ggctttgtcg
    17821 gccatggtca ttatcgtggg acgactggtg ctcgagggcc ggatgcgcta ccgctgggat
    17881 gccctcccac cgcgccgatc atgacctatt ggcgcaggct cttagtacaa cgccacagca
    17941 ttgggaacgc cagccggaca cctgctcaca agaagatggc acgactcggc tcacgctcag
    18001 gaaaccaccg ttgccacatg atcagggctg atggccttaa gggggcaacc tgcgactagc
    18061 ggaggtcact caggggcaga cgcgcaccgc cggcatagag caacggtacg tgtcggaggt
    18121 cgcagagcgt gcgcgagcca gtcgcgaaca ttgccagccg gagctcctcg accaggatac
    18181 ggagcatcgc cacggcagcc gcctcgctgt cggccgctgc cttcagcatc gggccggcga
    18241 acccaacgag gtccgcaccg agcgccaccg ccttggcagc atcgagaccc gtgcgcatcc
    18301 caccgctagc gaaaagaacg gcgttcggga gcgatgcgcg caccatccga aggctctccg
    18361 cagtcgggat gccccagtcg acgaaggtct cgctcagccg ccggtaatga tcatctcccg
    18421 cacggtgatg ctcgacggcg ctccacgagg tgccgccggc gccactgatg tcgatcgcgg
    18481 caacccccgc atcgtggagc tgtcgggcga catcggcgga gataccgaag ccgacctcct
    18541 tgaccaccac cgggacctcc agcgcgtcgc acaccttggt g
//
LOCUS       DASYWL010000137         4361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_344224, whole genome shotgun sequence.
ACCESSION   DASYWL010000137 DASYWL010000000
VERSION     DASYWL010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344224"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2166)
                     /locus_tag="VGR22_04920"
     CDS             complement(<1..2166)
                     /locus_tag="VGR22_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127943.1"
                     /translation="MHLVSNDYASTNDSVRTPVAGAANHFAAFIARGVDLARAALRRG
                     PVPDIHSESFFDDWQETWGVLRAASGLDEQRFWEFISDFELNLGVGPEAFPELGRTTP
                     ARRRDVGELADLLFGIVANPAHIVQLTHAELLERLGWTHRLTFRSQHVFPVPTAYQPN
                     EAAVADLLGAIETHTGGYLAVAAPAGAGKSSLLTATTLPARRTIHYYAYTPASPDPST
                     GRGESENFFFDLVTALDAAGLHTADVVDPRDRLANNDRLLRQLDLAGREWQDEGTRTI
                     VVIDGLDHIPREQNPSRSLIDDLPSPAQIPDGVFVVLGTQLIDILPVAVRTALSDDSR
                     IVEVPPLTDVTMLAIATAAGPGEWLQPEQKQRLIQMSEGHPLALTYLLEAFSAIAFDP
                     DDPGPAWSAASSVLGEGDLYRGSVLARYEGYWQHVADAADVRQLLATVSRIRSPLHLD
                     WLRTWSAPESVRRFADSAAPFFIKDGPSWSFIHNSFRQFLIKRTATVGGAFDPAEHRR
                     IHAEAAEVCAASVGAWESYRDEELAQRVHAEQHHQVLELASPERLRQRLLDLAPQAAI
                     ALDTELALRSAASLHDARAVLQLALMQFELELRESVLEPHDLAFALIDIGEYDVALSH
                     VIAGRELRLDQTRALEAAVRLRAADRIADAGAIVRAVGSVSTLLETSRQDRFYDRKTA
                     ERIQWWVAAHAQMRPAVEVLRDIDGALPASNGSDREQGED"
     gene            3598..>4361
                     /locus_tag="VGR22_04925"
     CDS             3598..>4361
                     /locus_tag="VGR22_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV2127944.1"
                     /translation="MTRPKRPARRIAAAAGLALAMLAVTSGAASAAAGTVTANKTLSA
                     TDIACNGSTDVTLTLQGETGIAGNPVDIVLVLDRSGSMAGAPLANMKTGANAFVDVID
                     EATDGVLDGVVANGSRVGVVSFSDNATVNEPLTTNAGALKADINALVAGGQTNHTAAI
                     NTAEAQLAGSNPASEKVMIIFTDGQSVPSSANGLAAANAAKAAGTEIFAIGLGSVNVG
                     QLNAWASDPDSEHVLIAPSSADLQAIFEAIGAAIVVP"
BASE COUNT          807 a         1410 c         1432 g          712 t
ORIGIN      
        1 gtcctctccc tgctcgcgat ccgacccgtt cgaagcggga agcgcaccgt caatgtctcg
       61 cagaacctcg accgccggcc gcatttgcgc gtgcgcggcg acccaccact ggatgcgctc
      121 cgcagtcttt cggtcataga agcggtcctg tcgtgaggtc tctagcaatg tgctgaccga
      181 tcccacggcg cggacgatcg cgcccgcatc ggcgatgcgg tccgcggcac gaagacgcac
      241 ggcagcctcg agagcgcgag tttggtccag gcgaagttct ctccccgcga tgacgtgcga
      301 tagcgcaacg tcgtactcac cgatgtcgat caacgcgaag gcgaggtcgt ggggctcgag
      361 gacggactct cgcaactcga gctcgaactg catcagggcg agctggagga cggctcgtgc
      421 atcgtgcagg gaagcggcag atcggagggc cagctccgtg tcgagggcta tcgccgcttg
      481 gggagcaaga tcgagcagtc gctgccggag ccgctcaggg ctggcgagct cgagaacttg
      541 atggtgctgc tcggcgtgta cgcgctgcgc aagctcctca tcgcgatagg actcccacgc
      601 gccgaccgac gccgcacata cttcggcggc ttcagcatgt atccggcgat gttccgccgg
      661 atcgaatgcc cctccgacgg ttgccgtccg tttgattagg aactgtctga agctgttgtg
      721 aatgaagctc cacgagggtc cgtctttgat gaaaaacggc gcggcggaat cggcaaaccg
      781 cctcaccgac tcgggcgcgc tccatgtgcg aagccagtca aggtggagcg gcgaccgtat
      841 ccgcgagacg gtggctagaa gttggcggac atcggccgcg tcagcgacgt gttgccagta
      901 gccctcgtag cgcgccagaa cactccctcg atagaggtcg ccttccccga gcaccgagct
      961 ggcggcggac caggccggac cagggtcatc cggatcgaac gcgatggccg agaacgcttc
     1021 gagaaggtac gtgagcgcaa ggggatggcc ttcgctcatc tggatgagcc gctgcttctg
     1081 ctccggttgg agccattcgc ctggaccagc ggcggtcgcg atcgcaagca tcgtgacgtc
     1141 ggtaagaggc ggcacctcaa cgatccggct gtcgtccgag agcgcggtcc tgactgccac
     1201 cggcagaatg tcgatcagct gcgtcccgag gacgacgaag acaccgtcgg ggatttgcgc
     1261 tgggcttggc agatcgtcaa tcagggagcg ggaagggttc tgttctcgcg gtatgtggtc
     1321 gagcccgtcg atgacgacga tcgtgcgcgt gccctcgtcc tgccactccc ttcctgcgag
     1381 atcgagctgg cggagcaacc ggtcattgtt cgcgagtcga tcgcgaggat ccactacgtc
     1441 ggccgtgtgc aagcccgcgg cgtcgagcgc ggtaacaagg tcgaagaaga agttctccga
     1501 ctctccccgc ccggttgacg gatcgggcga cgccggcgtg tacgcgtagt agtggattgt
     1561 gcgccttgcg ggaagcgttg tcgcggtgag gaggcttgac ttcccagcac cagcaggggc
     1621 ggccaccgcg agatagccgc cggtgtgtgt ctcgattgcg cccaacaggt ccgcgaccgc
     1681 tgcttcgttt ggttgatagg cagttggaac cgggaagacg tgctgacttc ggaacgtcag
     1741 acgatgtgtc catccgagtc gttccaggag ctcagcgtgg gtcagctgca cgatgtgggc
     1801 tgggttggcg acgattccga acaggaggtc tgcgagttcg cctacatctc gccgacgagc
     1861 cggcgttgtg cgaccgagtt ccgggaacgc ctctggtccg acaccaaggt tcagctcgaa
     1921 gtcagagatg aactcccaga agcgctgctc atcgaggcct gatgcagcgc gcagcacccc
     1981 ccaggtctct tgccagtcgt cgaagaacga ctccgagtga atgtcaggta cgggaccacg
     2041 ccgtagcgca gcgcgagcaa ggtcgacgcc tcgggcgatg aacgccgcga agtggtttgc
     2101 tgcgcctgca acgggagtgc ggacggagtc gttcgtggag gcgtagtcgt ttgacaccaa
     2161 gtgcacgcgc gtctctcgtc ccggatgggc ggaccggatt cgctcccatg ctcgtgcgag
     2221 atcaccgaca agggacggct ggcctgcaca gaactctcga aacgtgaacg caccgggaaa
     2281 gccgctccac tttacctgca gcgcgtgaat gggacctgcg cgcacagcaa gttgaaagtc
     2341 gtcagcgaca cctaccgatg gatccgcgac cttcaaccac tcgagatcgc gtaggcgcgc
     2401 gatcgtcagc tcagcggcaa cgcggtactg cgggaagtag cccgtcgctg cacgtcgctc
     2461 acctgcgccg ggttcgacca cttgtcatct ttctcgcggg tgtgatcgcg acgcgaccag
     2521 ccctccgctc gagatcgtct ccatggtccc gatggtacgc atccgccatc gagaagcgat
     2581 gccccgcgcc gtccgcgggc gtgccctcgc aacccagctc gtcaacatcg cgcacctagg
     2641 ggcgcgcgaa ccggcccaac gcgaatcagc tgagcgctcc gatccggcgt tacctggtcc
     2701 tggcaaaagc cgcgaacggg cggcagcgtc gtcgaagccg cccagtcggg aggtcgacag
     2761 gacagtgccg tggccagggc aggcctcacc ttcgatcgca agccggcctt cgcaacgtcg
     2821 ttggcctccg cgaacgtggg ggcgcgcgct tgcgcatcac ctctcagact tcgtcgtcgg
     2881 ctaggcagac cgggcgcagc cccggagggc gctgctggtg ctggcccgat cgggcaactg
     2941 tttctggacc atcccgtcac cggcctgcag acaagatcca gattgggccg aaacttctaa
     3001 cgcttcttct aacgcacacg ccggcacggg ccgtcacagc ccgaaatagg cgacacagcg
     3061 cggcacaaga aagaccaggt cagaggcggt tttgggccct catggcatgg actgacacga
     3121 ccttaagggg tctcataagc cgaaggtcgc cggttcaaat ccgtccgtcg agccgctgcg
     3181 cgccgtcaag cgccgggtcc cacccggtca ctgatgggag tccctgctcg agatcttcgt
     3241 cacgaggagc tactagcgag ttcttcaact ggctgaagaa atgggctcgc ccggggtggc
     3301 gacgctcgta gccatgacgc tctacggcat cgtgtacggc gtatgggcaa tgtcccgccc
     3361 gattcgggac agctgcctct agtcaacggg acaacacgcc cgggatgctg caagggacat
     3421 acacgccggg tgggatcggc gaggcgagtc cctgatgcgg ggccacggcc accggaacgg
     3481 cggcgagaca gaccccaacg cgccaccgca tgagaaagga agaatccgat gaagggttcc
     3541 agacaagggg ccggtgtctc accgcctttg ggaacgagca gggccgaggc ccaacccgtg
     3601 acacgaccca agaggccagc tcgtcgcatc gctgcagcag ccggcctggc gctcgccatg
     3661 ttggctgtca cgtcgggagc ggcctcggcc gcagccggca cggtgaccgc gaacaagacc
     3721 ctcagtgcta ccgacatcgc ctgcaacggg tcgacggacg tgaccctcac cctccagggc
     3781 gagaccggca tcgccggcaa ccccgtggac atcgtgttgg tcctggaccg ctccggcagc
     3841 atggctgggg cgccgttggc caacatgaag acgggagcca acgccttcgt cgacgtgatc
     3901 gacgaagcga ccgacggggt cctcgatggc gtggtcgcca acgggagccg cgtcggcgtg
     3961 gtcagctttt ccgacaacgc caccgtgaac gagccactga ccaccaacgc cggggcgctc
     4021 aaggcggaca tcaacgccct ggtggccggc ggccagacca accacacggc ggccatcaac
     4081 actgccgagg cccagctggc cgggtccaat cccgccagcg agaaggtgat gatcatcttc
     4141 accgacggcc agagcgttcc gtcgtcagcc aacggcttgg ccgcggccaa cgctgccaag
     4201 gcggcgggga ccgagatctt cgccattggc ctgggcagcg tcaacgtggg ccagctcaac
     4261 gcctgggcca gcgacccgga cagcgagcac gttctcatcg cacccagctc cgccgacctg
     4321 caggccatct tcgaggccat cggcgccgcc atcgtggtgc c
//
LOCUS       DASYWL010000138         1636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_81641, whole genome shotgun sequence.
ACCESSION   DASYWL010000138 DASYWL010000000
VERSION     DASYWL010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81641"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..1335
                     /locus_tag="VGR22_04930"
     CDS             154..1335
                     /locus_tag="VGR22_04930"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2127945.1"
                     /translation="MTLEASEFQRIAARVAERASELLCTPVLVTNEQQVVVASSDPAR
                     LGHAFAADDAITCEPCTRLSLHLDGHDGEVVVTEPGEHEATSPRLTQALIDLVVNQVT
                     VVDRLPDKYQLKDKLIYDLLHGVGDPAALLREAKVLAMDLTTPRAVILIDAAEFILRP
                     GPVVRDEPDSGAPRRRAQLIIGSTASFFQLPDDSICAYLGDGEIGVLKASNSSSLDPW
                     VDVTDGVGQPSASWSNLTALKRAAEALLVRLRGDTRSTINIGVGRYHPGVRGLAASYQ
                     DARAALSLGRRVQGQDGVHCLDSLGIAAFVGVADEQTKLDLAAHLLSPLDHEPELIRT
                     LDVFFAEDCCPSTTAGNLGIHRNTLSYRLDKVRSLTGLDPRRFDQAVQVRLALLLRSL
                     R"
BASE COUNT          281 a          564 c          501 g          290 t
ORIGIN      
        1 tagcacttca tcgacgagag cggcagccga agatagcgct acggaagctc atatcggctc
       61 gagattgccg agtgggcgcg gcattggctg aactcgcgct gacattgatt ggggaagtca
      121 ctggcgcttc aggcgaggtg aacgaagggg ttcttgactt tggaagccag cgagttccaa
      181 cggatcgcag ccagagtcgc tgagcgtgcg tcggaattgc tctgcacacc cgtgctggtc
      241 accaatgagc agcaggtggt cgtcgccagc agcgatccgg ctcggctcgg gcacgccttc
      301 gcagcagatg acgcgatcac ctgcgagcct tgcacgcgcc tctcgctcca tttggacggc
      361 cacgacggcg aggtggtcgt tacggagccc ggcgagcacg aggcaacatc gccgcgactg
      421 acccaggccc tcatcgatct cgtggtcaac caggtcacgg tcgttgaccg cctccccgac
      481 aagtaccagc tcaaggacaa gctgatctac gatctcctcc acggggtcgg tgatccggcg
      541 gcgctcctgc gcgaggccaa ggtcctcgcc atggatctca ccaccccgcg tgccgtgatc
      601 ctgatcgacg cggccgaatt catcctccgc ccggggccgg tcgttcgtga tgaacccgac
      661 agtggtgcac cccgtcgccg cgcccagctg atcatcggca gcaccgcctc gttcttccag
      721 ctgcccgatg attccatctg tgcctacctc ggagacggtg agatcggggt gctcaaggcg
      781 agcaattcaa gcagcctcga cccctgggtc gacgttacgg atggcgtcgg tcagccgagc
      841 gcctcctggt cgaatctcac agccctcaag cgcgccgccg aggccctcct ggtccggctc
      901 cgtggcgata cccgttccac aatcaatatc ggcgtcgggc gctatcatcc cggagtccgg
      961 ggactcgctg cctcctacca ggacgcgcgt gcggcgctct cgctcggccg ccgcgtccaa
     1021 gggcaagatg gggtccactg cctcgacagc ctggggattg ccgcattcgt cggcgttgcc
     1081 gacgagcaga cgaagctgga cctcgccgcc catctcctca gcccgctcga ccacgagccg
     1141 gagctgattc gcaccctcga cgtcttcttc gccgaggact gctgcccctc gaccacggcc
     1201 ggcaacctcg ggatccatcg caataccctc tcctatcgcc tggacaaggt gcgttccctg
     1261 accgggcttg atccccgccg gttcgatcag gccgtccagg tccgtctcgc cctcctgctc
     1321 cgctcgctgc gatagctcgc tgccgccagc gccattgggc gtttgcccaa gaactcacgc
     1381 tcaacctctc cgatggttgt gcgtctgccc aatgcattat ccgcgggacg ccgttatgat
     1441 caggagaagc ggcgcggtcc gaggaggccg cgaccgtgat gtcatgatct ggagactcgg
     1501 cagagaggga gggcatgcga catggagcgc ctgtcccacc ggagcaaatg cccgagcacc
     1561 agccggacca ggaggtcgcc agtgacagac catcccgtgg ccgactcgtc tcgcttcatt
     1621 cagcgggcgg cagagg
//
LOCUS       DASYWL010000139         7014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_569630, whole genome shotgun sequence.
ACCESSION   DASYWL010000139 DASYWL010000000
VERSION     DASYWL010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7014
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569630"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(417..647)
                     /locus_tag="VGR22_04935"
     CDS             complement(417..647)
                     /locus_tag="VGR22_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127946.1"
                     /translation="MPHRTQRDHDKDQDIDRAGERGGDTGPSSYVDDGPQAEKGDARR
                     DKPVHSGPTRRGTSRNKSKHDKRSGSDSNASN"
     gene            1153..1824
                     /locus_tag="VGR22_04940"
     CDS             1153..1824
                     /locus_tag="VGR22_04940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEV2127947.1"
                     /translation="MPEVIVFDVNETLLDVGALDPFFEDRFGNAGVRREWFGQMLQSA
                     FVTTITGDYHDFGALAMGALTMVASRHGVTLSDADRKELGASMRGLPPHPDVRPAMER
                     LRDAGFRLASLTNSTLEVARAQLENAELMSLLEAALSADTVHRLKPAPEPYRMAAETL
                     GVEIRDLRLVAAHSWDVAGALSAGCAATFVARPGQVLDPAIPEPDMIASDIAGVAERL
                     IARNA"
     gene            complement(1829..2542)
                     /locus_tag="VGR22_04945"
     CDS             complement(1829..2542)
                     /locus_tag="VGR22_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127948.1"
                     /translation="MTSLRVGALVVILLFAAGTPAILAMQATPEPAATPVTQPVGLVY
                     PPAAIIHGATLGEWNARYWQWVTSLPVGENPGQDATGELCGYGQNDPVFFIPGNMPPC
                     TVPAGIAIFLPVAGTECSTLEPPPFHGEGEDELAACAAADGDRYTNISVVVNGEEIPE
                     VERFRAASPAFTLQLPEENILAVSAETGRAVADGYALVLAPLPPGEHEIVMHVEVVDG
                     YALPDKTLRLTVVKPDAEG"
     gene            complement(2539..3207)
                     /locus_tag="VGR22_04950"
     CDS             complement(2539..3207)
                     /locus_tag="VGR22_04950"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEV2127949.1"
                     /translation="MIGRVRWTTTAVLLLLALPWLVPVALGQQVDAGCPVDDADIVID
                     PGHGGDDPGAQQETYALDEAALNLEIAERVAELLRDEHLLAVALTRTDNGTTLGNSER
                     GDIANACGADLFVMIHLNAAFDPEQDYTVTLWGEKTKDLAFSQVMLRSLASLGIPMAD
                     SWQFDNGALLRARMPSVLVEGAFMTNEAEAMDLANGVRQQWIAQAVVQGVVDWLELTG
                     ERAR"
     gene            3318..5219
                     /locus_tag="VGR22_04955"
     CDS             3318..5219
                     /locus_tag="VGR22_04955"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEV2127950.1"
                     /translation="MHGRRSLLTVMATAMLALAIALGFIMTPAARQGTTGVNGDDGPL
                     SAVLPEHRETIAQQTDTMPRYTIAATLLLPPEPTATQPVPIASPLASPVASPVAATPK
                     LPETFTEPRIVGTLDLHYVNTTGEALDDLWLRLYPNLRQYGSGEMAVANVQVDGEPVE
                     VQPPPLHSVPDATPIAIEDAGERDLAILRIPLATSLTPGEAVSLRMDFETTVPIDPPD
                     ENGLFRYTPETGTWTLAHGIPMLAGHDSEAGWVTDPPAAWSDITFSNTALFDITLTAP
                     ADLVLVTTGVEVGSTESDGYRTERIVTGPVRDVAIIADPNLESTSTEVAGTTITSWHR
                     PGEEAGSEQILQWAAQSFAIFTELFGPYPYEALDLVPVPHAIGYEFPQLVFIGSDFYP
                     DPEALGSRPGAIEFLVAHEVAHQWWYGLVGSNPHSNAFLDEGLTEYSTIVYFERQHGE
                     EAAASHLRDGLIHRYGFMLVTTGDQVVDQPTVDFPDAGAYYATVYRKAGLGFQAIREE
                     IGDDAFFTALRSYAEEHRFDVATPTDLLAAFEEASGQQLDDLWTLWFESDRGRIEIVM
                     EPLPGTPPAATPATTAPATPAIPPAATPAQGMPASPAPSPATISATPVSPIGSPAATP
                     VAPPAAAVG"
     gene            5546..5995
                     /locus_tag="VGR22_04960"
     CDS             5546..5995
                     /locus_tag="VGR22_04960"
                     /inference="COORDINATES: protein motif:HMM:NF016024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rad52/Rad22 family DNA repair protein"
                     /protein_id="HEV2127951.1"
                     /translation="MAVEELNPVQPLSPAGERSADEILDLLGRRFRPEVVQTRKIGGR
                     QVSYLPVSVVTERLNKACNSWNFRIVSRDRELMQLNRWNEATRRSEPREVQVSIVVGE
                     LTIPELGTRQAIGVQALDDGSGEDLIKGAASDSLKKCASLFGVSVDG"
     gene            6298..>7014
                     /locus_tag="VGR22_04965"
     CDS             6298..>7014
                     /locus_tag="VGR22_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127952.1"
                     /translation="MRGRTALHHANHRQTAGVAGQPIRLRNLMRSGIASRISWLIAIV
                     IAALAVGWLALVILFDSVRFVVLNPNAKTGFEVFLAIGQIFGAFVLAISPLEPARIRL
                     RWVAAGFLVFGVGGLAYGYLYPLLVDSPHLTATMYGSLYVRTVGTALCAAGLALGTLP
                     RIERSILILLTAAAILCGALLIPLADHLPPLIRISGTTEIIDHDPVIDATDPTDHVHL
                     SDLEALLSDTPRTVPGLTAWH"
BASE COUNT         1210 a         2437 c         2105 g         1262 t
ORIGIN      
        1 gtacagccgg gtcgcttgga agatatcccc tgatgcgccg cacgaccagc acttgaagtt
       61 gtcgtagccg aggctcacag tcaggctcgg gttggtgtct cgatggaacg gacagatgcc
      121 acggaaacgc tggtgaccca ccggcttcag gtcgacgcca actacgacgg tgaagaactt
      181 atcgacgggc cagatggcct tgaggctacg cgcctgctcc agcagcgact cgtaccactt
      241 acccttggca tcgggccacg attgatcatt gcaccgccgg tagacgtccg cgcgacggtc
      301 aagctcagcc gtgagcgggt tcgtgattcg agtccctggc agttcgcaaa gatgtgcccc
      361 cggggttggt ccccgggggt ttctcgtgcc cgttcgcgtg ggttactcac accgaatcaa
      421 ttcgatgcat tgctgtcaga gccggagcgc ttatcgtgct tgctcttgtt gcgggatgtc
      481 ccgcggcgcg tcgggccgct gtgaaccggc ttgtcccgcc gggcatcacc cttctcagcc
      541 tgcgggccgt catccacata cgacgagggt ccggtgtcgc caccgcgctc ccctgcccgg
      601 tcgatatcct ggtccttgtc gtggtctcgc tgcgttcggt gcggcatggt tgtcctcctg
      661 ctacgtcgtc gagccgtcgg cagtccaccc gcagaatctg tgccgggcat ttgctaaccg
      721 aacaccgacc ctccggctcc attcgcgtgc cctccacaga gaccaatact taactactgc
      781 atacatgacc atcctgctgt tcccacgcct gatacccccg atcggggatt cgcaagcggt
      841 atccgtacaa atacgtacag cgccttggta atccaacgca tgtgtcgagg cggcgtcgcg
      901 agattatcga gtccctccgc tgcgattgaa tcacatgcta tccatgccgc tgacagcgac
      961 gccctggaat acctccctga gtccccaagc acacgagcgg cagcatcagt cgccggtgct
     1021 agactacaga cagtgccgag ccggtactgg tgaggctgtc ccacgtacaa caccgcagtc
     1081 cttgaccggt ccaacacgag cctgccgcga ccgaggttcc gaaaactcgt ccactgcaga
     1141 ctggagagct tcatgccgga agtcatcgtt ttcgacgtca acgaaacgct gctcgatgtc
     1201 ggcgcacttg acccgttctt cgaggatcgc tttggcaatg ccggcgtccg ccgcgagtgg
     1261 ttcggacaga tgctgcaatc ggcattcgtc acgaccatca ccggcgacta ccacgacttc
     1321 ggcgctctgg cgatgggcgc gctgacgatg gtcgccagcc gacatggggt aacgctttcg
     1381 gacgcggacc ggaaggaact cggagcgagc atgcggggtt tgccaccgca ccctgacgtt
     1441 cgcccggcca tggagcgtct ccgcgacgca ggctttcgcc tcgccagtct gaccaactcg
     1501 acgctggagg tcgcccgcgc ccaactggag aacgccgagc tcatgtcact gctcgaggcc
     1561 gctctctcgg cggacacggt gcatcgcctc aagccggcac ccgaacccta ccgcatggcg
     1621 gcagagacgc tgggcgtgga gatccgcgac ctccggctcg tggcggcaca ctcatgggat
     1681 gttgccggtg cgctgagcgc gggatgcgcc gcgaccttcg ttgcccgtcc cggacaggtc
     1741 ctcgatccgg caattccgga gcctgacatg atcgcgtcgg acattgccgg cgtggccgag
     1801 cgactcatcg cccgaaacgc gtgatcgctc aaccctccgc gtccggcttg acgacggtca
     1861 agcgaagcgt tttgtctggc agcgcgtatc catccaccac ctcgacgtgc atcacgattt
     1921 cgtgctcgcc cggaggcaac ggggcgagta cgagcgcgta cccgtcggcg actgcgcgtc
     1981 ctgtctccgc cgacacggcg agaatgttct cctccggaag ctgaagcgtg aacgctggcg
     2041 aggctgcgcg gaagcgctcc acctccggta tctcctcacc gttcacgact acggagatgt
     2101 tcgtgtagcg gtcgccatca gcggcggcgc aggccgccag ctcgtcctca ccctcgccat
     2161 ggaacggtgg cggctccagc gtggagcatt cggtaccggc gaccggcagg aagatggcaa
     2221 tgcccgccgg aacggtacag ggcggcatgt tgccggggat gaaaaatacc gggtcgttct
     2281 gcccgtagcc gcacagctca ccagtcgcgt cctgcccagg gttctcaccc accggcagac
     2341 tggtgaccca ctgccagtac cgcgcgttcc actctccgag ggtggctcca tgtatgatcg
     2401 cggctggcgg gtaaacgagc ccgactggct gggtgaccgg tgtcgccgcc ggctccggcg
     2461 tggcttgcat cgccaggatc gcgggggtac cggcggcgaa gaggaggatc acgacgagtg
     2521 cgccgactcg gagtgaggtc atcgcgctcg ctccccggtc agctcgagcc agtcgaccac
     2581 cccctgcacg accgcctggg cgatccactg ctgccgcacg ccattagcaa ggtccatcgc
     2641 ctcggcctcg ttggtcatga aggccccctc aaccagcacg ctcggcatcc gcgcgcgcag
     2701 caacgctccg ttgtcgaact gccacgagtc cgccatagga atgccgaggg acgccaatga
     2761 gcgcagcatc acctggctga aggccaagtc cttcgtcttc tcgccccaaa gcgtgaccgt
     2821 gtagtcttgc tcgggatcga aggcggcgtt gagatggatc atcacgaaga ggtctgcccc
     2881 gcaggcgttg gcgatgtcgc cccgctcgct gttgccgagt gtcgtgccgt tgtcggtcct
     2941 cgtgagcgct actgcgagaa ggtgttcatc gcgcagcaat tcggcgaccc gttcggcgat
     3001 ctcaaggttg agcgccgcct cgtccagcgc gtaggtctcc tgctgcgcgc ccggatcatc
     3061 gcctccgtgg ccgggatcga tcacgatatc agcgtcgtcg acagggcacc cggcatcgac
     3121 ctgctggccg agcgccaccg gcaccagcca cggcagcgcg agcaggagca gcaccgctgt
     3181 tgtcgtccac cgtacccgtc caatcatcgc ttccaccatt tctgcattcg cgagcctgcc
     3241 tgctacggta tcatacggaa gccggagggc gacgaaacac aaggtgccag cagtatcacc
     3301 actcggagga gtctttcatg cacggacgac gatcgctcct caccgtcatg gccacagcta
     3361 tgctggcact cgcgatcgcg ctcggcttca tcatgactcc ggctgcccgg cagggaacaa
     3421 cgggcgtcaa cggtgacgac ggccccctct ccgctgtact gcccgaacac cgggaaacca
     3481 tcgcccagca aacagacacg atgccacgct acaccatcgc ggcgaccctc ctgctcccac
     3541 cggagccaac cgccactcaa cccgttccca tcgccagccc gctggcttcg ccggtcgcgt
     3601 cgccggtggc agcgaccccc aagctgccgg agacgttcac cgagccccgc atcgttggca
     3661 cgctcgacct gcactacgtc aacacgaccg gcgaggcgct cgacgatctt tggctccggc
     3721 tctacccgaa tttgcggcag tacggcagcg gcgaaatggc ggttgccaat gtgcaggttg
     3781 acggggagcc ggtcgaggtg cagccaccgc ccctccactc ggtgccggac gcgaccccga
     3841 tcgccattga ggacgcgggt gagcgtgacc tcgctatcct gcgcatcccg ctggcgacga
     3901 gcctcacgcc gggtgaagcg gtctcccttc gcatggactt cgagaccacc gtcccaatcg
     3961 acccgcctga cgagaacggc ctcttccggt acacgccgga gacgggcacg tggacgctcg
     4021 cgcacgggat cccgatgctc gccgggcatg actccgaggc tggatgggta accgacccgc
     4081 cggcggcctg gagcgacatt accttcagca acacggccct cttcgacatc acactcaccg
     4141 caccagccga tctcgtgctg gtcaccaccg gcgtcgaagt cggatccacg gagagcgacg
     4201 gataccgcac cgaacgaatt gtcactgggc cggtccgtga cgttgccatc atcgcggatc
     4261 cgaacctgga atcgaccagc accgaggtgg cgggcaccac catcacgtcc tggcaccggc
     4321 ccggcgaaga ggcggggagc gagcagatcc tgcagtgggc ggcacaatcg ttcgcgatct
     4381 tcaccgaact cttcggcccc tacccgtacg aagcactcga cctggtaccg gtgccccacg
     4441 ccatcggata cgagttcccg cagctcgtct tcatcggcag cgacttctac ccggatccgg
     4501 aggcactggg gagccgaccg ggagcgatcg agtttcttgt cgcgcacgag gtggcgcatc
     4561 aatggtggta cgggctcgtc ggcagcaacc cccactccaa cgcctttctc gacgaagggc
     4621 tgactgagta cagcacgatc gtctacttcg agcgccagca cggcgaggag gcggcggcct
     4681 cccatctgcg ggacggtctc atccaccgct acgggttcat gctggtcacc accggtgatc
     4741 aggttgtcga tcaaccgacg gtcgacttcc ctgatgccgg ggcgtattac gcgacggtct
     4801 atcggaaggc agggctcggg ttccaggcga tccgcgagga aatcggcgac gacgccttct
     4861 tcacggccct ccgcagctac gccgaggagc accggttcga cgttgcgacg cccactgacc
     4921 tgctggctgc ctttgaggag gcttccgggc agcagctcga cgacctctgg accctgtggt
     4981 tcgagtctga ccgcggtcgt atcgagatcg tgatggagcc gcttcctggg accccgcctg
     5041 ccgccactcc cgcgactacg gcccctgcta caccggcgat cccgcctgcg gctacgcctg
     5101 ctcaggggat gccggcgtcg cccgctccga gcccggcgac aatcagtgcc acaccggtct
     5161 cgcccatcgg atcaccggcg gctacgccgg tcgcgccccc tgcggcggcc gtcggctgac
     5221 cggcaggata ccggctaccg cagcccccac cacagtaccg agcgctggtt ggctcgctcc
     5281 gcgaccgacc accgctttcc ctctgagata gacgtcgccg gcctgcctcc aaccacgcgc
     5341 tctccctgcc cagcaggcac acctcgcttc aaccagcttt cccgacctcc tgtgctacac
     5401 tggtttccga acgtatgttc tattttccgg tatactgtga gtgtgaccgg agctcggtgc
     5461 cgcgcggact gcacccaccc gcgggccgag cgccgtcact atcagagcag cgcatgagcc
     5521 ccagagtgac aggagagcga gcatcatggc ggtcgaagag ctcaatcccg tgcagcccct
     5581 cagccccgcc ggggagcgca gcgccgacga gatcctggat ctgctgggac ggcggttccg
     5641 gccagaggtc gttcagacgc gcaagatcgg cgggcgacag gtctcctacc tgccggtttc
     5701 cgtggtgacc gagcggctga acaaggcgtg caatagctgg aacttccgca ttgtctcacg
     5761 agaccgggaa ctgatgcagc tcaaccgctg gaatgaggcg acccgccgca gcgagccgcg
     5821 cgaggttcag gtcagcattg tcgtcggcga gctgacgatc cccgagctcg gcacccgtca
     5881 ggcgattggc gtccaagcgc tggacgacgg ctcgggcgag gatctcatca agggcgcggc
     5941 ctctgacagt ctcaagaagt gtgccagcct ctttggcgtc tccgtcgacg gctgatctgc
     6001 cgcttctcac tccagaatag tagccaatta gcactgcggg aatgactctc cgccgtgttt
     6061 tccctttcct ccaagccgag caagcgccct tggtcaatgg ctccgtcggg ctagacttcc
     6121 cggttgccca cgaacgaggc tatagtatcc gcgggggcag cacacactcg ggagacgacg
     6181 cgacaccagg gcggttcgca ggtgcagggc cgtttctcct cgcgatgcac cgctctttgc
     6241 cgagtgtagc cgtcagcaat gttcgacgag atcgattgct tggggagcag attctacatg
     6301 cgtggaagaa ccgcacttca tcacgcgaac caccgccaga ccgccggtgt ggccggccag
     6361 ccgattcgcc tgcgcaacct catgcgttcc ggcattgcga gtcgcatatc atggctcatc
     6421 gcgattgtta tcgctgcgct ggcagtcggc tggctggcgc tcgtcatcct ctttgactcc
     6481 gtgcgcttcg tggtactcaa cccgaacgcc aagaccgggt tcgaggtctt cctggccatc
     6541 ggccagatct tcggcgcctt tgttctcgcg atctcacccc tcgagcccgc acgcatacgg
     6601 ctccgctggg tagcagctgg cttcctcgtc ttcggcgtcg gcggattggc ctacggttat
     6661 ctctatccgc tgctcgtcga ctcgccccat ctgaccgcca ccatgtacgg ctcgctctac
     6721 gtgcggacgg tgggcaccgc tttgtgcgcg gcaggactcg ccttgggcac cctccctcgc
     6781 atcgaacggt cgatcctgat cctgctcact gcagcggcaa ttctctgcgg cgccctcctg
     6841 ataccactcg cggatcacct gccaccgctg atccgcatct ccggcaccac agagatcatc
     6901 gatcacgatc ccgtcatcga tgccaccgac ccgaccgacc acgtccatct cagcgacctc
     6961 gaggcgctcc tgtccgatac gccgagaacg gttccgggac tcacggcctg gcac
//
LOCUS       DASYWL010000140         3370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_231387, whole genome shotgun sequence.
ACCESSION   DASYWL010000140 DASYWL010000000
VERSION     DASYWL010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231387"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..1742)
                     /locus_tag="VGR22_04970"
     CDS             complement(216..1742)
                     /locus_tag="VGR22_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEV2127953.1"
                     /translation="MSGIIASRGRIDPEQGERMLDRLVHRGPDGSGSAVVGESWLGHR
                     WLAIVEDSQQPIRSEAAGLTLIADGEVYNAAELRDACGDVAFATRDTVETALHLYATS
                     GPEAFAEMDGQFALVTAGDDGRFVAARDPFGLKPLFWARRDRQTLFASELSAYDEDWM
                     PLAEFFPPGHYWTPQEGLVRFAYAVPDAPAMATPFERPEEEGDPIPEEILERVRKELE
                     EAAEQQMMGNAPIGAFLSGGLDSSVIAALAARWCEQCGQRLKTFAVGFEGAPDLAAAR
                     VVASHLGTDHYEKTYTADEALEVLPEVVLALENFDPSLVRSSVSNYIVAELAAQHVKV
                     VLCGEGADEIFGGYHYLKEFDSEEALHAELVRGIEGGHNGGFQRVDRTTMIHSLQARM
                     PFMARDNIALGFAIPAAWKLASDVMPEKHVLREAFQGWLPHDILSRKKAQFGEGSGAV
                     DVLQERMTESVTEEELQRERDVVAPPLRTREELAYYRIFAEHFPGAQAEKVVGRFVTA
                     "
     gene            complement(1904..2947)
                     /locus_tag="VGR22_04975"
     CDS             complement(1904..2947)
                     /locus_tag="VGR22_04975"
                     /inference="COORDINATES: protein motif:HMM:NF022646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HEV2127954.1"
                     /translation="MPFDADARRALLERHANDWLRVGIENLHREFPHKPNLIITEPGP
                     IRTHRECHPVFFGSFDWHSCVEMYWMMVRIMKLVPGLPNETAAREAIETLLTEEGMAQ
                     ELATFRDPSSRSMERPYGWGWYLTFVHELATWDDGDAGCWLERSRPLADHFTAGFVQW
                     LPKLHYPQRFGMHANTAFGLARSWGWAGHLTDQGDDTLREAIREAALRYFAADTDYPA
                     HYEPSGADFLSGALTEAELMSLVLQSGEFPAWLERFLPGIHAAQPDVLFHPVVVTDTS
                     DGQLAHLEGLNLSRAAAFVAIADALPVADVRIDPMLDAAERHADASLGQASGSNYMVE
                     HWLATYAVLLLGQ"
BASE COUNT          561 a         1146 c         1074 g          589 t
ORIGIN      
        1 cctcaccgtc gagcgtcgcc agcgcgatcg cgaattgctc ctgctcgggc tccggcatgg
       61 ccggcttgtg atagccgatt cccggcgtgt agtaggaggt cacctcgtcg cgtattgcga
      121 gtagtgccgc cggtagagct cctcgagccg gttgtcgaca cgttcggtct gctcgaccga
      181 cagctgtgtg ctcgtgggtt ggttcatgtc actcatcatg ctgtcacgaa ccggccgacc
      241 accttctccg cctgtgcacc ggggaagtgc tcggcgaaga ttcgatagta ggcgagctcc
      301 tcgcgcgtgc gcagcggcgg cgcgacgacg tcccgctcgc gctgcagctc ctcctcggtg
      361 acgctctccg tcatgcgctc ctgcagcaca tcgaccgcgc cggaaccctc accgaattgc
      421 gccttcttgc gcgagaggat gtcatgcggc aaccagccct ggaatgcctc gcgcagcacg
      481 tgcttctccg gcatcacgtc gctggccagc ttccacgccg cagggatcgc gaagccgagg
      541 gcgatgttgt cccgcgccat gaagggcatg cgcgcctgga ggctatggat catggtggtg
      601 cgatcgaccc gctggaagcc gccgttgtga ccaccctcga tgccgcgcac aagctcggcg
      661 tgcagcgcct cctcgctgtc gaactccttg agatagtggt agccgccaaa gatctcgtcg
      721 gcgccctcac cgcagagcac taccttgacg tgctgcgctg cgagctcggc gacgatgtag
      781 ttggataccg agctgcgcac cagcgaggga tcgaagttct ccagcgccaa cacgacctcc
      841 ggcagcacct cgagtgcttc gtcggcggtg taggtcttct cgtagtgatc ggtgccgaga
      901 tggctggcga ccacccgcgc tgccgcgagg tcgggcgcgc cctcgaagcc gaccgcgaac
      961 gtcttgagcc gctgcccgca ctgttcgcac caccgagcgg cgagcgcggc gatcacgctg
     1021 gagtcgagcc cgccggagag gaacgcgccg atcggcgcat tgcccatcat ctgctgctcg
     1081 gccgcctcct ccagttcctt gcgcactcgc tcgagaatct cctcggggat ggggtcgccc
     1141 tcttcctccg gtcgctcgaa tggtgtggcc atggccggtg catccgggac ggcgtaggcg
     1201 aaccgcacca ggccctcctg cggggtccag tagtgcccgg gcggaaagaa ctccgccagt
     1261 ggcatccagt cctcgtcgta ggcgctcagc tcagatgcga agagggtctg tctgtccctg
     1321 cgcgcccaga agagtggctt gagcccgaag gggtcgcgcg cggcgacgaa ccggccgtca
     1381 tcaccggcgg tcaccagcgc gaactgtccg tccatctcgg cgaaggcctc cgggccgctc
     1441 gtcgcgtaga gatgcaacgc ggtctcaacc gtgtcgcggg tagcgaacgc gacgtcaccg
     1501 caggcatcgc gcagctcggc ggcgttgtag acctcgccgt cggcgatcag cgtcagcccg
     1561 gccgcctcgg atctgatcgg ctgctggcta tcctcgacga tcgctagcca gcggtggccg
     1621 agccagctct cgccgactac tgcgctgccc gagccgtccg gcccgcggtg gaccagccga
     1681 tccagcatgc gctcgccctg ctcggggtcg atccttccac ggcttgcgat aattccactc
     1741 atcggtgtgt ggtgctcctg atggctcgtc atggtttcac ggcggccagt aattgccgat
     1801 cggcgatgat tctgcccggt tggacgggcg acatccaacg cgccgagcgc gcaagaacgg
     1861 gaccagatac tctgctgaac agacagagtg tgccctctgc tgcctactgg ccgagcagca
     1921 ataccgcgta agtcgcgagc caatgctcca ccatgtagtt gctgccgctc gcctgtccca
     1981 gtgatgcatc cgcatgccgc tcggcggcat cgagcatcgg atcgatgcgc acatcggcta
     2041 ccggcagtgc atcggcgatc gcgacgaagg ccgccgcgcg gctcaggttg agcccttcca
     2101 gatgggcaag ctgaccgtcc gatgtgtcgg tgaccacaac tgggtggaag agcacgtccg
     2161 gctgtgcggc atggatcccg ggcagaaacc gctccagcca tgccggaaat tcgcctgact
     2221 gcagcaccag tgacatcaat tcggcctccg tcagcgcgcc cgagaggaag tcggcgccgg
     2281 agggctcgta gtgcgccgga tagtcggtgt cagcagcgaa gtagcgcagc gccgcctccc
     2341 ggatcgcctc gcgcagggta tcgtcccctt ggtcggtcag gtgaccggcc cagccccacg
     2401 atcgcgccag cccgaaggcc gtgttcgcat gcatgccgaa tcgctgcggg taatggagct
     2461 tcggcagcca ctggacgaag cccgcggtga agtggtctgc cagagggcgc gaccgttcca
     2521 gccagcaccc ggcatcgccg tcgtcccacg tcgccaactc atgcacgaag gtcaggtacc
     2581 agccccagcc gtaggggcgc tccatgctcc gagaggaggg atcgcggaac gttgccaact
     2641 cctgcgccat gccctcctct gtgagcagcg tctcgatcgc ctcgcgggcg gctgtttcgt
     2701 tcggaaggcc cggaaccagt ttcatgatgc ggaccatcat ccagtacatc tcgacacagg
     2761 aatgccagtc gaacgagccg aagaagaccg ggtggcactc acggtgggtg cggatcggtc
     2821 ccggctcggt gatgatgagg ttgggtttgt gcgggaactc acggtggagg ttctcgatcc
     2881 ccacgcggag ccaatcgttg gcatggcgtt ccagcagcgc acgacgcgcg tcggcatcga
     2941 agggcatggc tgacgtctcc tcacgcatgc gtgttgaacg acacggcgtt catgctcgca
     3001 cccaaacggt actgcacacc ctgtgcagcc gccctggtcg acgggaatcc gcccaacggc
     3061 acaatacgaa tacgtacacc tcataagaag tacagccggg tggctcggcc ccgagccaat
     3121 cccgccccgg ttccccgcac cagcatcggc cgagatcgct cggccgcgga tttgccggcg
     3181 ctgcgccgat gcgcagcccg tgacggatca aacgttgatg catcctggcc aggccggggg
     3241 aaaggaaccg agaccatgac gaccctattg atcgacgccc tgctcgtctt ctggctgctg
     3301 ctcttcggtg ccatggccct cctgccggtg cttaccggca ccaccagcgc caagcgccaa
     3361 tcggatcgcc
//
LOCUS       DASYWL010000141         4057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_63111, whole genome shotgun sequence.
ACCESSION   DASYWL010000141 DASYWL010000000
VERSION     DASYWL010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63111"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..916)
                     /locus_tag="VGR22_04980"
     CDS             complement(158..916)
                     /locus_tag="VGR22_04980"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV2127955.1"
                     /translation="MKQDDATISIPQIVTVATMNVGNGMAPDDQLVKVVRQVQPDILA
                     FEELNHRQAEHLAEKLADIWAHQFSFGDGYEGRAIFSRFPLLRQEMLPITSDRPDGLV
                     EVELDGQALTIIVGHPRPPKFRGRGLETPYSSHRQLLRLADIAVERSPAVLLGDFNVR
                     PDNGIYERLRQRGLVDAFAESGEGPERTFPVRVANEIRTFGRRTVRVKTPPVFRADYI
                     WHTPDIRSVATWVGADTGSDHLPVVSRLAIPPKP"
     gene            complement(1171..1758)
                     /locus_tag="VGR22_04985"
     CDS             complement(1171..1758)
                     /locus_tag="VGR22_04985"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEV2127956.1"
                     /translation="MLSILLRSWWVLILRGLFAIMFGLIVLVWPDITVFVIVTIMGAF
                     LMLDGMIEIWVGFLGRGRDEHWWTDAFLGIVAVLAGVAVIAWPGITAVGLMIFIGAMI
                     AVYGATMLYQAIRLRNEMTGEGLLIANGGIALVLGVSFMIFPGAGAISLVYLIAAFFI
                     VFGLLLVMLGWKLHGVLGDLRDEATRQETALRQEP"
     gene            complement(2011..2754)
                     /locus_tag="VGR22_04990"
     CDS             complement(2011..2754)
                     /locus_tag="VGR22_04990"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV2127957.1"
                     /translation="MAIDSVFLLACLIMFTATVVSGLAGFGLSIVSIPPLLLLYEPGT
                     AIALNKILTLGTTWIIIVETWKHISWRWISRILPLVLAGLFAGSWLLRVLDPNVIKVI
                     AGATVFALAVLLLAWHPHALPERPWMAPLVGLLSGAASTSTGMAGPPLVLFFTLMAVG
                     KQTFRATSAMYFITLDIVGLPTLIGQGSVSGSDLRLALALAPVAVIGRLLGAKLVPYV
                     SPLAFRRATLGLLLVTGTVSMVTGLSALL"
     gene            complement(2754..3518)
                     /locus_tag="VGR22_04995"
     CDS             complement(2754..3518)
                     /locus_tag="VGR22_04995"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV2127958.1"
                     /translation="MSFLDIPYDTTTVVAIALIVYAMTALSAIAGFGFGTVAVPMLLL
                     VLPPAATVAVVKVVGTGTGWIVLLSIWRRIQWLTVVKILPAALVGLVFGGYILKTSRP
                     STIQLIVGVLVLFSAATLVVRPILIENDAIWSTSLIGFLTGVMGNATGLLAPAVVVYF
                     TGRQFPKDVFRATTLSLFLIIELVRLPTLLLQGAITLEDIRFALLLVPAAVAGRLSGL
                     RLVRYVPAAQFRALVIALLFVMATTSILSGAQGLIR"
     gene            3544..3672
                     /locus_tag="VGR22_05000"
     CDS             3544..3672
                     /locus_tag="VGR22_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127959.1"
                     /translation="MAGSAAMGAGNTDEVARRLAGRWGKPVVTEIDLPDRRIRSRS"
BASE COUNT          795 a         1255 c         1379 g          628 t
ORIGIN      
        1 cgaggcctga acggccgagg tgaagtgccc cgcgcgaagc accagcgccg caggtgtcgt
       61 tctcgtttta cccggatttc gtatcaatgt agcgatatga tgggtgggtg gtctgctcag
      121 gggatccacg acgacccaaa ttgacaccgc atattggtca gggtttcggc gggatggcga
      181 ggcgggacac gacgggaaga tggtccgagc cggtgtcggc gccgacccag gtcgccaccg
      241 atcggatatc gggcgtgtgc cagatgtagt ccgcacggaa gaccggcggg gtcttgactc
      301 ggacagtccg ccggccgaac gtcctgatct cgttggcaac gcgcacgggg aatgtgcgct
      361 ccggcccctc gcccgattcg gcaaaggcat ccaccagccc acgctggcgc agccgctcgt
      421 aaatgccgtt gtcgggccgc acattgaagt cgcccagcag tacggccggg gatcgctcca
      481 ccgcgatgtc ggccagccgc agcagctgcc ggtgtgagga gtagggcgtc tccagccctc
      541 gccccctgaa cttgggcggg cgcgggtgac cgacgataat ggtgagcgcc tgtccgtcga
      601 gctcgacctc cacgaggccg tccggacggt cgctggtgat cggcagcatc tcctgtcgca
      661 gcagagggaa ccggctgaag atagcgcgcc cctcgtagcc gtccccgaag ctgaactggt
      721 gcgcccagat gtcggccagc ttctcggcga ggtgctccgc ctggcgatgg ttgagctcct
      781 cgaaggcgag gatgtcgggc tgcacctgcc ggacgacctt cacgagctgg tcgtccggcg
      841 ccatgccgtt gccgacgttc atcgtcgcga cggtcacgat ctgtgggatc gagatcgtcg
      901 cgtcatcctg tttcatgtca ggttccactg tctctccggg gtacggccgg cacccatcgt
      961 ttcagatggc gatgcagtgc ctctgcatcg acgatcgact ccgcgccaag gtcgagatcg
     1021 cgagggtgca gcaggaacgg ctccgtctgg aagccgccca gcgtaccgtg cgagccaacc
     1081 ggctcctcgg acaccggcgt ctcgccggtg tccggattat acgcactgtg tacgagccga
     1141 ccgggtgcat cggcaagtcc acgtgaaata tcacggttct tgccgaagag cggtttcctg
     1201 tctcgtggct tcgtcccgca ggtcaccgag cacgccgtgg agcttccatc ccagcatgac
     1261 aagcagcagg ccgaagacga tgaagaacgc ggcgatgagg tagacaagac tgatggcacc
     1321 cgcgcctggg aagatcatga agctgacgcc gagcaccagt gcgattccac cgttcgcgat
     1381 cagcagccct tcgccggtca tctcgttccg gagccggatt gcctggtaga gcatggtggc
     1441 gccgtagacg gcgatcatcg cgccgatgaa gatcatcaga ccgacggcgg tgatgccggg
     1501 ccaggcgatc accgcgacgc cggccagcac ggcgacgatg ccgagaaaag cgtcggtcca
     1561 ccagtgctcg tcgcggccac gtccgagaaa gccgacccag atctcgatca tgccgtcgag
     1621 catcaggaag gcgcccatga tggtgacaat gacgaacacg gtaatgtccg gccagaccag
     1681 gacgatgaga ccgaacatga tggcgaacag gccgcgcagg atcaggaccc accaactccg
     1741 caggagaata ctcagcatcg gagatgtcac tcccgtcatg acacagcgtc gattttcctc
     1801 ggtggcctag cctaccagac cggcaagggg atggtgagga cacatggccc ttccttggtc
     1861 cgccagtggc cggaaatccg tcgcggatgt caccaatcga cgacgcgaag accaggtggt
     1921 cgtcgcccgg agcgaggcaa ggccggcgcg aagggtgccc tgcgaggcgt agtgttatcg
     1981 gtgatgggtg ggatcgctcg tcagcagcgt tcacaagagg gccgagaggc cggtcaccat
     2041 cgagaccgtt cccgtcacca gcagcagccc gagcgtcgcc cggcggaacg ccagtgggga
     2101 gacgtagggc accagcttgg cgcctaggag ccgtccgatg actgccaccg gggccagggc
     2161 gagcgccagg cgcagatcgc tcccgctcac cgatccctgg ccgatcagcg tcggcaggcc
     2221 cacgatatcg agagtgatga agtacatcgc gctcgttgcc cggaacgtct gcttgccgac
     2281 ggccatcagc gtgaagaaga gcaccagtgg cggtccggcc atgccggtcg aggttgacgc
     2341 cgcaccgctc agcagcccga ccagcggtgc catccacgga cgctcgggca gcgcgtgcgg
     2401 gtgccacgcc agcagcagca ctgccagcgc gaagacggtc gcgccggcga tcaccttgat
     2461 gacgttgggg tccagcaccc gcagcagcca gctcccggcg aagagcccgg cgagaacgag
     2521 cggcaggatc cgggagatcc atcgccacga gatatgcttc cacgtctcga caatgatgat
     2581 ccacgttgtt cccagggtga ggatcttgtt gagcgcaatc gccgttcccg gctcgtagag
     2641 cagcaacagc ggcgggatcg agacgatcga gagcccgaac ccggcgagcc cggagacgac
     2701 cgtcgccgtg aacatgatca ggcaggcgag caggaagacg gaatcgatgg ccatcagcgg
     2761 atgagcccct gcgcgccgct gaggatgctc gtcgtcgcca tgacaaagag gagcgcgatc
     2821 accagcgccc ggaactgtgc cgccgggacg taacgcacca gtctcagccc gctgagccga
     2881 ccggcgaccg ctgccgggac cagcagcagc gcgaaacgga tgtcctcgag cgtgatggca
     2941 ccctgcagca gcagtgtcgg cagcctgacc agctcgatga tgaggaagag cgacagggtg
     3001 gtagcccgaa agacatcctt ggggaactgg cggccggtga agtagacgac cactgccgga
     3061 gccagcaggc cggtggcgtt gcccatcacg ccggtcagga agccgatgag tgaggtcgac
     3121 cagatggcgt cgttctcgat cagaattggc cggacgacga gcgtggctgc cgagaacagc
     3181 accagcactc cgacgatgag ctggatcgtt gacggacgag acgttttcag gatgtaccct
     3241 ccgaaaacga ggcccaccag tgccgccgga aggatcttga cgacggtaag ccactgaatc
     3301 ctgcgccaga tcgacagcag cacgatccag ccggtgccgg tgccgacgac cttcaccacc
     3361 gcgaccgtgg ccgccggtgg cagcaccagg agcagcattg ggacggccac cgtgccgaaa
     3421 ccgaacccgg cgatggccga gagcgccgtc atggcgtaga cgatgagggc gatggcaacg
     3481 accgtcgtgg tgtcgtacgg gatgtccagg aagctcacgc ggtgcaccca ggcaagtggt
     3541 tcggtggcgg gaagcgcagc gatgggggct ggcaacaccg atgaggtcgc ccgacgactg
     3601 gcgggacggt ggggcaagcc ggtggtgaca gagatcgacc tgccggatcg gcgcatccgg
     3661 agtcggtcat gagtcgatga cctcgtggtg ggcggcaggg gttcgcgcgt gctggcggcg
     3721 accatgaggc atcgtaccaa aggatgccgt cgtacggacc attccctttc cccaccgcgc
     3781 tcgcggcatg ttgcgccaag tctgggagat gccagccggc gtcgggtatt gccggagcca
     3841 ttgagtttct gccggggtcg tgggttcgcc gagtggaggg cagtgtgacc aagggggcga
     3901 gcgccggcgg aggcgcaaga cccggtcgga ttggcccagg gacgacggca ggcatagtga
     3961 aaccgtctca caaatggtcg tccgctgtag tcttgacata gcgataatca cacgcgcatc
     4021 atggatcttg cgcctaatgt gacatgagcg atggatc
//
LOCUS       DASYWL010000142         7215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_119263, whole genome shotgun sequence.
ACCESSION   DASYWL010000142 DASYWL010000000
VERSION     DASYWL010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7215
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119263"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VGR22_05005"
     CDS             complement(<1..711)
                     /locus_tag="VGR22_05005"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEV2127960.1"
                     /translation="MTHLPDDEDALDHIRVFTPEEEVDPDEAEVAEEPKPALDEVIAA
                     LIGGEYPRSVLVGLSDLGVRDAAELDRAWPQIDVGIRRAVPVELSDLAEERVDYVFHR
                     ALATLLDDPDPAVRQRAIAGLWEDEDLRLASSLATMVRDDESEDVRAEAARGLGKFVE
                     RIELGEIESRVGEEVLGALRDALEDDTESMHVRARALESLSVISDDESMREAIDRFYT
                     EDETGFRATAIFAMGCSAD"
     gene            complement(695..2464)
                     /locus_tag="VGR22_05010"
     CDS             complement(695..2464)
                     /locus_tag="VGR22_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127961.1"
                     /translation="MVAEQTLPELTYSGNDPEKSLAERVFQVIRAQGMFASNYAPIRV
                     RLGELAAFLNASEQDVRGAVEANGDIFVLEDDEAATWVVSTRLGTPPVEMVPDSRHTF
                     AERLMTPEPAPERPLRTERPIPEMFVPTEVGAAIGDAREEEVEVEAELRDEGIEVDED
                     ITVELVTPEAAPEAPAEAEMVESEPVGEEEAEVAPEAVVEAAPEVVEEAEEAPEAAVG
                     VSDLSNYDDDAVAEAVREALRNDSRVANFADQWMAEDRVERLSRGNLRGLADYINEQE
                     QPLTDDVLVQDVLGVRRSASDFETMRFSMNYRLSSERNFEFLGTPDQPFWGTTDLPPI
                     GTTRRKASDIGTDYRYLVDEVPASSVEPRSVKTVQHTLSFYEFTYGVLPLDAQMQALL
                     PAPMMPDQRSAVLMVEIPQFENSVYLVEVRYPTHNRGGFILGLDDFYEEKLVPGAMLS
                     IEATDNDGQYKIEYLEDEERQDRFLELDDRRAPKYVFRPLSYDTAIDSDWLITEDRFP
                     RFGNEKPLSERQRRRIEEVIEETFRRVGDQDGDNWYATFTDLLVAVNVERPASETMLR
                     EAIESMPSVTDDGSGMLTYDPSA"
     gene            complement(2556..2822)
                     /locus_tag="VGR22_05015"
     CDS             complement(2556..2822)
                     /locus_tag="VGR22_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127962.1"
                     /translation="MCTAFAQLEGRTLQYLLIAHGMGCPSRFVVRILFPEIQETKRRM
                     ANKSATPALNSEEIAVLRDALRATSRPMTTAELVSALRQAAANR"
     gene            2931..4172
                     /locus_tag="VGR22_05020"
     CDS             2931..4172
                     /locus_tag="VGR22_05020"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2127963.1"
                     /translation="MALAVPKTAVFRNRPLMLLMAGHFTIDMYGGMLPLLFPLLTAQF
                     DLDLATVGLVSLAYTGVSSLSQPVFGYIADTYGTRFTGLTMAWTAILFAGIGFAPNFP
                     ALLVLAALAGFGSGAFHPLGALNASAVIPDKQRNSAMSVYVTGGTVGFALGPLIGAAV
                     FALFGMQGTAVMIAPGVLIAVWLLGSLRANATKGSRYRTVDDPPLSAPPLPLATISIV
                     ILVMMLRMVPIVGVQTFIPVWYERLGYGPSFYAWLATTVVLSSAVGAIGAGQLADRFG
                     RRTVILVTLVATIPAVWLFADFPGPWAFVTGAMVGLLAASTAPLLLVMAQQLMIGRAG
                     MASGLILGLSFIAGAVSAPLFGRLADAVGLQNAMRSLPLIVLATIGVAWFLPDERRMA
                     EVTNRPEKPVTPSPSPAPTRG"
     gene            4309..4539
                     /locus_tag="VGR22_05025"
     CDS             4309..4539
                     /locus_tag="VGR22_05025"
                     /inference="COORDINATES: protein motif:HMM:NF022829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HEV2127964.1"
                     /translation="MFDKMKNAASNLGDVGKMGDLKQYVDDISFPATKEEIIGHLRAN
                     GAQEDLIGKLREVGQEHFTSQSDLLTSFMGKR"
     gene            4824..5792
                     /locus_tag="VGR22_05030"
     CDS             4824..5792
                     /locus_tag="VGR22_05030"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEV2127965.1"
                     /translation="MHRVPRLLLVFMLVIGSMLAVATASAQDEEEDLRFVMVTHGQAA
                     DPFWSVVQRGAERAAEDLGVTVEYQAPTTFDMVAMSQLIDAAVASNPDGLVVSIPDAD
                     ALSPSIQAAVEAGIPVISINSGAEVADELGLMTHVGQTEYEAGFGGGQRMGAAGATEA
                     ICLIHEAGNIGLEERCRGFSEGLAETGGTGEQLVVDLNNPTEAQQRIEAALTSNPDIN
                     GIMALGPTGALPAMQAIEAAGVGDRVQLATFDLSPEVLQGIQDGTVLFAIDQQQYMQG
                     YLPIVFLTLYQRNGNVPADDVVLTGPGFVDSSNVDQVIELVEEGTR"
     gene            5864..6928
                     /locus_tag="VGR22_05035"
     CDS             5864..6928
                     /locus_tag="VGR22_05035"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2127966.1"
                     /translation="MSVSDSKAGESGLRRDERLRQTGLIRGTLLRPEVGAIMGAAALW
                     AYFAIVAGDRGFLSWRGTISYLEVSAQLGILSVAVALLMIGGEFDLSIGSTIGASGMI
                     IAIASVNYELNLWLALVVSLIVAIAIGLANGVLVRWSGLPSFIITLATLFIIRGLTIA
                     LTRIETGRTQLGGVEDTAGFDLARSVFGSDLQLFGTRFPVSILWWLGLTALATWVLLR
                     TPFGNWIFGSGGNEQAARNIGVPVTRVKITLFVGTAVAAWLVAVVQVISTTGADVLRG
                     TGQEFFAIIAVVIGGTLLTGGYGSAIGAAIGALIFGMVRQGIVYAGVDADWFQVFLGG
                     MVLIAVLINRYVRKRAMEAR"
     gene            6925..>7215
                     /locus_tag="VGR22_05040"
     CDS             6925..>7215
                     /locus_tag="VGR22_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2127967.1"
                     /translation="MTVRADGTPLIEVHNISKSFGNVQALRDVSMCVYAGEVHCLLGD
                     NGAGKSTLIKALSGVHAPDTGEILIQGKPVRFGSPRDALRSGIATAYQDLAMI"
BASE COUNT         1208 a         2460 c         2199 g         1348 t
ORIGIN      
        1 gtccgcgctg cagcccatcg cgaagatcgc cgtggcacga aagccggtct cgtcctccgt
       61 gtagaaacga tcgatcgcct cgcgcatcga ctcgtcgtca ctgatgacgg agagcgactc
      121 cagcgcccgg gcgcgaacgt gcatcgactc ggtatcgtct tccagcgcgt ctcggagcgc
      181 cccaagcacc tcttcgccca cccgactctc gatctcgccg agctcgatgc gctcgacgaa
      241 cttcccgaga ccgcgggctg cctcggcccg gacgtcctcc gactcgtcgt cgcgcaccat
      301 cgttgccaag gaggaagcga gacgcaagtc ctcgtcttcc cacagtccag cgatcgcccg
      361 ctgacgaacg gcggggtccg ggtcgtcgag cagcgtcgcg agcgcgcgat ggaacacgta
      421 gtccacccgc tcctccgcga gatcggacag ctcgacgggc accgcccgcc ggataccgac
      481 gtcgatttgc ggccaggccc ggtccagctc ggctgcgtct cgcacgccaa gatcagagag
      541 accaaccaat acgctgcgcg gatactcacc cccaatgagc gcagcgatca cctcgtcgag
      601 cgcaggcttg ggctcttcgg ccacctcggc ctcatccgga tccacctcct cctcgggagt
      661 gaagacccgg atgtggtcga gggcatcttc gtcgtcaggc agatgggtca taggtcaaca
      721 tgcccgaacc gtcgtcggtc accgacggca tcgactcgat tgcctcgcgg agcatcgtct
      781 cggacgccgg ccgctccacg ttcacggcga ccagcagatc ggtgaaggta gcgtaccaat
      841 tgtcgccgtc ctgatcgccg acacgccgga acgtctcctc gatgacctcc tcgatgcggc
      901 ggcgctggcg ctcggagagc ggcttctcat tgccgaatcg tgggaagcga tcctcggtga
      961 tgagccagtc ggaatcgatc gccgtgtcgt agctgagcgg ccggaagacg tacttcggcg
     1021 cccggcgatc gtcgagctcg aggaagcggt cctgcctctc ctcgtcctcg aggtattcga
     1081 tcttgtactg gccgtcgttg tcggtcgcct cgatcgagag catcgcgccc ggaaccagct
     1141 tctcctcgta aaaatcgtca aggccaagga tgaagccgcc gcggttgtgc gtcgggtagc
     1201 gaacctcgac gaggtagacc gagttctcga actgcgggat ctcgaccatc aacacggcgg
     1261 atcgctggtc cggcatcatc ggtgccggca gcagcgcctg catctgggca tcaagtggca
     1321 gtacgccgta ggtgaactcg tagaacgaga gcgtatgctg gaccgtcttg acggagcgcg
     1381 gctcgacgct actcgccggg acctcgtcga cgaggtagcg atagtcagta ccgatgtcac
     1441 tcgccttgcg gcgagtcgtg ccgatcggcg gcaggtcggt ggtgccccag aacggctggt
     1501 ccggtgtgcc gaggaactcg aagttgcgct ccgacgagag ccggtagttc atcgagaacc
     1561 tcattgtctc gaagtcactc gcacttcgcc gcacgccaag aacatcctgc accagcacgt
     1621 cgtcagtcag cggctgttcc tgctcgttga tgtaatcggc gaggccccgc agattgccgc
     1681 ggctgagccg ctcgacccta tcctcggcca tccactggtc ggcaaagttg gcaacacgtg
     1741 aatcgttgcg cagcgcctcg cggaccgcct cggcgaccgc atcgtcgtcg tagttgctca
     1801 ggtcactgac accgacggcc gcttccggcg cctcctcggc ctcctcgaca acctctggcg
     1861 ccgcctcgac gacagcctcc ggagcgactt cggcctcttc ctcgcccaca ggctcagact
     1921 cgaccatctc ggcctccgca ggtgcctcgg gagcagcctc cggcgtcaca agctcgaccg
     1981 tgatgtcctc atcgacctca atgccctcat cccggagctc ggcttcgacc tcgacctcct
     2041 cctcgcgggc gtcgccgatt gcggcaccga cttcggtcgg cacaaacatc tctggtatcg
     2101 gccgctcggt tcggagggga cgctcgggtg ccggttccgg tgtcatgagc cgctcggcga
     2161 aggtgtgcct cgaatccggc accatctcga ccggtggagt gccgagccgt gtggaaacga
     2221 cccaggtcgc cgcctcgtca tcctcgagga cgaagatatc tccgttcgcc tcgacagcgc
     2281 cacggacatc ctgctccgac gcgttgagga acgcggctag ctcgccgagc ctcacgcgga
     2341 ttggcgcgta gttggatgca aacatgcctt gtgcgcgaat cacctggaag acgcgctcgg
     2401 cgagcgactt ctcaggatcg ttgccgctat acgtcaattc gggaagtgtc tgttcagcca
     2461 ccatgcgcgt gactctcctg tcgtgacaat gcatcccccg taagtcccga ctttcatcgc
     2521 caagcgtggc gaggaagccg gtacgggcga gacgatcagc gattggcagc tgcctggcgc
     2581 agcgcactga caagttcagc agtggtcatc ggacggctgg tcgcgcggag tgcatcgcgc
     2641 agtacggcaa tctcttcgct gttcaatgca ggcgtagcgc ttttattcgc cattcgtctc
     2701 ttcgtctcct ggatttctgg aaagaggatg cgcaccacaa aacgggatgg gcagcccatc
     2761 ccatgcgcga tcaatagata ttgtagagtc ctgccctcca attgcgcaaa cgcggtgcac
     2821 atcgcgcacc ttgaccggca ctccggtcga gggtacaaca cgcccgggca ccgccccgac
     2881 gactcctccg cgacgctcac gctcgatcac gggccgagga catcgcctcc atggcactcg
     2941 ccgtacccaa gacggccgtt tttcgcaaca gacccctgat gctgctcatg gcgggccatt
     3001 tcacaatcga catgtacggc gggatgctgc ccctgctttt cccgcttctc acagcacagt
     3061 tcgatctcga tctcgccacc gtcggcctgg tgtccctcgc ctacaccggt gtctcgtcgc
     3121 tctcacagcc cgttttcgga tacatcgccg acacctacgg gacgcgcttc accggcctga
     3181 caatggcctg gacggcgatc ctcttcgccg ggatcgggtt cgctcccaac ttcccggcgt
     3241 tgctcgtgct cgccgctctg gcaggcttcg gatccggggc attccaccca ctcggcgcgc
     3301 tgaacgccag cgccgtcatt cccgacaagc agcgcaacag cgcgatgtcg gtctacgtta
     3361 ccggtggcac ggtcggtttc gcgcttgggc cgctgatcgg cgccgccgtc ttcgccctct
     3421 tcggcatgca gggcacggcg gtgatgatcg cgcccggcgt gctgatcgcc gtctggctcc
     3481 tcgggagcct gcgcgcgaac gcgacaaagg gctcccgcta tcgcaccgtc gacgaccccc
     3541 cgctctcggc ccctccccta ccactggcaa cgatcagcat cgtcatcctc gtgatgatgc
     3601 tgcgcatggt gccgatcgtt ggcgtgcaaa ccttcattcc cgtctggtac gaacggctag
     3661 gctacggtcc cagtttttat gcgtggctcg cgacgaccgt cgtactctcc agcgccgtcg
     3721 gcgcgatcgg cgccgggcaa ctggccgacc ggttcggtcg tcgcactgtc atcctcgtca
     3781 cgctggtcgc aaccatcccc gccgtctggc tcttcgccga ttttccgggt ccgtgggcat
     3841 tcgtcaccgg cgcgatggtc gggctgctcg ccgcctcaac ggcgccgctg ctgctcgtca
     3901 tggcgcaaca gctaatgatc gggcgcgccg gcatggcatc cgggctgatc ctcggtctga
     3961 gcttcatcgc cggggcggtc agtgccccgc tcttcggcag gctcgccgac gccgtcggcc
     4021 tgcagaacgc catgcggtca ctgccactga tcgtgctggc taccatcggg gtcgcctggt
     4081 tcctgcccga cgagcgccgt atggccgagg tcaccaatcg tcccgagaaa ccagtcacac
     4141 cgagtccctc ccccgccccc acccgcggtt gagcgacaac cgccgcccgc cctgaacatc
     4201 ggcgcgccgc ggccccgcag atcgccacct gctcaccatg gcactgtgct tgctcattgc
     4261 gtccgggcgg gcaatcggca ccgctacgca cctgtgaagg aggtacagat gttcgacaag
     4321 atgaagaatg ccgcttccaa cctcggtgat gttggcaaga tgggcgacct caagcagtac
     4381 gttgacgaca tcagctttcc ggcgaccaag gaggagatta ttggacacct ccgcgccaat
     4441 ggcgctcagg aggacctgat cggcaagctg cgcgaggtcg ggcaagagca cttcacgagc
     4501 caaagcgact tgctcaccag cttcatgggc aagcgatagg ccatctctct atgcagcacc
     4561 gatcgccccg ccggaacccg gcggggcggt tttctgtgtc ccactgacca cggctgttcg
     4621 caagcaccga ctcagggtgc tgtagtatgg tgcaaggcgt acatggacga cgggacggat
     4681 cgcgcggtat ggaccgcttg cgcccgccat catgcatctg gtatggctgg gccggctcag
     4741 cggcccccaa tgcgacccca gcccgttggc cgcgccctag gaccaacagt cccagtagaa
     4801 gcacgaatcg aggaggactc gctatgcatc gtgtaccccg cctgttgctc gtcttcatgc
     4861 tcgtgatcgg gtcaatgctc gctgttgcca ccgccagtgc ccaggacgag gaggaagacc
     4921 ttcgcttcgt catggtgacc cacggccagg cggccgaccc gttctggtcc gttgtccagc
     4981 gcggtgccga gcgagcagcc gaggatctcg gcgtgaccgt tgaataccag gctccgacca
     5041 ccttcgatat ggtcgccatg tcgcagctca tcgatgctgc ggtagcctcc aacccggatg
     5101 gtctggttgt ctccattccc gacgccgacg ccctctcgcc ttcgatccag gcggcggttg
     5161 aggcaggtat tcccgtcatc tcgatcaact ccggtgccga agtagccgat gagctcggcc
     5221 taatgaccca cgtcggacag accgaatatg aagccggttt cggtggcggc cagcggatgg
     5281 gtgccgcggg ggccaccgag gcgatctgcc ttatccatga ggccggaaac atcggtctcg
     5341 aggagcgctg ccgcggcttc tccgagggtc tcgccgagac cggcgggact ggagagcagc
     5401 tcgtcgttga cctcaacaac ccgaccgagg cgcagcagcg tatcgaggcg gcgctgacat
     5461 cgaacccgga tatcaacggc atcatggcgc tcggaccgac gggcgcactg cccgcaatgc
     5521 aggcgatcga ggccgccgga gtgggcgacc gtgtgcaact ggccacgttc gacctctcgc
     5581 cggaggtgct gcaaggtatt caggacggca cggtgctgtt cgcgatcgac cagcagcagt
     5641 atatgcaggg ctacctcccc atcgtcttcc tgaccctcta ccagcgcaac ggcaacgtcc
     5701 cggcagatga cgtcgtccta acgggccctg ggttcgtgga ctccagcaac gtcgatcagg
     5761 tcatcgagtt ggttgaagaa ggcacgcgct agcaacgacg cgggcgggag ggcaatgacg
     5821 ccctcccgcc cattttccat ccagtgcagg agacacccct gccatgtccg tttccgactc
     5881 caaggcaggc gagtccgggc tgcgccgcga cgagcgactc cgccaaacgg gcctgatccg
     5941 gggaacgttg ctgcgcccgg aagtcggtgc catcatgggc gctgccgcgc tctgggccta
     6001 cttcgccatc gtcgccggcg atcgcggctt tctgagctgg cgaggcacca tcagttacct
     6061 cgaggtgtcc gcccagctcg gcatcctttc ggtcgccgtc gccctgctca tgatcggcgg
     6121 cgagttcgac ctctcgatcg gctcgacgat cggtgcgagc ggaatgatca tcgccattgc
     6181 ctccgtgaac tacgagctga acctttggct cgcgctcgtg gtctcgctca tcgtggcgat
     6241 cgcgatcggg ctcgccaacg gcgtgctggt gcgctggtcg ggcctgccat cgttcatcat
     6301 caccctggcg acgctcttca tcatccgcgg cctgacgatc gccctcacgc ggatcgaaac
     6361 cggccgtacc cagctcggcg gggtcgagga cacggcgggc ttcgatctcg cccgaagcgt
     6421 cttcggctcc gatctgcagc ttttcggcac caggtttcca gtttccatcc tctggtggct
     6481 cggattgaca gccctggcaa cgtgggtgct gctgcgcact ccctttggca actggatctt
     6541 cggctccggt ggcaacgaac aggccgcccg caacatcggc gtgcctgtga cgcgggtcaa
     6601 gatcaccctc ttcgtcggca cggcagtcgc ggcatggctc gtcgcagtgg tccaggtcat
     6661 cagcactact ggtgcggacg ttctgcgcgg caccgggcag gagttcttcg cgatcatcgc
     6721 ggtcgtgatt ggcggcacgt tgctgaccgg cggttacggc tcggcgatcg gtgcagcgat
     6781 cggtgcgctc atcttcggca tggtccggca gggcatcgtc tacgctgggg tcgacgctga
     6841 ctggttccaa gtcttcctcg gcggcatggt gctgatcgca gtgctcatca atcggtatgt
     6901 gcgcaagcgg gcgatggagg cgagatgacc gtacgagcag atggcacccc gctgatcgag
     6961 gtccacaaca tctcgaagtc attcggcaac gtgcaggcgc tgcgcgacgt ctcaatgtgc
     7021 gtctacgcgg gtgaggtcca ctgcctgctc ggcgacaatg gtgcgggcaa gtcaacgctc
     7081 atcaaggcgc tcagcggcgt ccacgcgccc gacaccggcg aaatcctgat ccagggcaaa
     7141 ccggttcggt tcggatcgcc acgcgacgcc ctccgctccg ggatcgccac cgcctaccaa
     7201 gacctggcga tgatt
//
LOCUS       DASYWL010000143         2183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_438360, whole genome shotgun sequence.
ACCESSION   DASYWL010000143 DASYWL010000000
VERSION     DASYWL010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438360"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(327..689)
                     /locus_tag="VGR22_05045"
     CDS             complement(327..689)
                     /locus_tag="VGR22_05045"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2127968.1"
                     /translation="MNAVRAQEVLTPDQAADYLHVNRATIYRYIRDGRLLASRLGRSY
                     RIPRRNLDLSLDANRTRRDITLREFSAREIADFLSADCLDGEVADIAARIERWANRPA
                     ATGRGLTEEPPSFLMHRV"
     gene            complement(805..1902)
                     /locus_tag="VGR22_05050"
     CDS             complement(805..1902)
                     /locus_tag="VGR22_05050"
                     /inference="COORDINATES: protein motif:HMM:NF022623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HEV2127969.1"
                     /translation="MTQFNALGMHLGNLSRLSAAETRSISPENPTGEPGRGGMATTGT
                     GADAARELGQGWKVSPSINIGGHETVTLADIDGAGAVQHVWLTVHPQHWRRLVFRCYW
                     DDEEQPSIEVPVGDFFCSGWGVRCNVSSLPVAVNPAGGFNSYWEMPFRSRARITLENL
                     SPDEVRGCYYQITYTLTDVPPDRSYLHAQWRRSNPLAAETVHTLLDGVTGQGHYVGTY
                     LAWGVNNDGWWGEGEVKFYLDGDGDWPTICGTGTEDYFGGAWNFEHPPGQYGVYSTPY
                     LGLPQVIVPDGLYRTQQRFGMYRWHVMDPIRFKERLRVTVQALGWRSQLDGKRRYLAR
                     QDDIASTALWYQTEPHAPFPALPGINDLEVV"
BASE COUNT          403 a          680 c          708 g          392 t
ORIGIN      
        1 gagaggtccg ccttccagca aaccccttcc acagatgacg actggcggac caccgtattc
       61 tacggcatgc cttcctcctg gcccgcggtg gtgccgatgc ccaggggcac cgtcatcgcc
      121 tatttgcgtg agcgtggcgc atacgggagg ggcgcgtgca ggctagcgtg atgggagaac
      181 gaggtcatca gattcacgat accacgggga cagcaagccc ttcggttcga tcagcacggg
      241 ctgcgaaacg gcggcgacga gcagtgagcg agcgcacagc gagagcagaa agctggatcc
      301 acctgtcggc gatccggcgg ccgcaatcaa acacgatgca tcaaaaaaga tggtggttcc
      361 tcggtcaatc ctcgtccagt ggcagcgggc cgattcgccc agcgctcgat tcgagcggcg
      421 atgtcggcca cctcgccatc aaggcaatca gcactgagaa aatcggcgat ctcccgcgcg
      481 ctgaattcac gcagggtaat atccctgcga gttcggttgg catcgagcga caaatcgaga
      541 tttcgacggg gaatacgata tgatcggccg agtcgtgacg ctagcagccg gccatcgcga
      601 atatagcgat agatagtcgc ccggttgaca tgaagataat ctgccgcctg atccggtgtg
      661 agaacttctt gcgcacggac cgcgttcatc ggatttcctc tctctgcttc catactgatc
      721 gccactgatc gccaacgcga tgctatcagg cgctcgcctt cacctttagg ttcggatcac
      781 gtcagcatgg ccgaactcac ggcatcacac gacctccagg tcattgatgc ctggcaacgc
      841 cgggaacggt gcgtggggct cggtttggta ccagagggcg gtggaggcga tgtcgtcctg
      901 gcgagcgagg tagcggcgct tgccgtccag ctgggagcgc cagccgagcg cctggacggt
      961 cacgcgcagc cgctctttga accggatcgg gtccatgacg tgccaccggt acatcccgaa
     1021 gcgctgctgc gtccggtaga gcccgtcggg gacaatcacc tggggtaagc cgaggtacgg
     1081 cgtggagtag acgccgtact ggccgggcgg atgctcgaag ttccaggcgc cgccgaagta
     1141 gtcctccgtg ccggtgccgc agatggtcgg ccagtcgccg tcgccatcga gatagaactt
     1201 cacctcgccc tcaccccacc agccgtcgtt gttgacgccc caggcgaggt aggtgccgac
     1261 gtagtgaccc tgcccggtga cgccgtcgag cagcgtgtgc accgtctcgg ccgcgagcgg
     1321 gttggaccgc cgccactgcg cgtgcagata ggatcggtcc ggtggcacat cggtgagcgt
     1381 ataggtgatc tggtagtagc aaccccggac ctcgtccggg ctcaggttct ccagggtgat
     1441 ccgggcgcgt gaccggaacg gcatctccca gtagctattg aacccgcccg ccggattgac
     1501 ggccaccggg agcgagctga cgttgcagcg gacgccccag cccgagcaga agaaatcgcc
     1561 aaccggcact tcaatcgacg gttgctcctc gtcatcccag tagcaacgaa agacgagccg
     1621 gcgccagtgt tggggatgga cggttagcca gacatgctgt accgcgccgg cgccgtcaat
     1681 atcggcgagg gtcaccgtct catggccgcc gatattgata ctcggcgaga ccttccagcc
     1741 ctgcccgagc tcacgagcgg cgtcggcacc ggtccccgtg gtcgccattc caccgcgtcc
     1801 cggctcgccc gtcggattct cggggctgat cgaccgggtt tcggctgccg agagccggga
     1861 gaggttgcct aggtgcatgc cgagcgcgtt gaattgagtc acagccgatc cgtccttcac
     1921 gagattgctt ccctcccact cgattctcga tccccgcgta ccggcctcct gccggtagcg
     1981 gacgcatcat agagcaggaa tgtgggaatg gcatcgtctc gggcgcaggg cagctggagg
     2041 acgacggtga ccagcgtgct atggccagct tggctcgtcc attggcgtca cgatgacttc
     2101 ctgcatcgtg gagccggatg gcatgcggac ggcgaagagg acggtctcgg cgacgttcgc
     2161 ggggtcctga agtgcggctt cgt
//
LOCUS       DASYWL010000144         2334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_44019, whole genome shotgun sequence.
ACCESSION   DASYWL010000144 DASYWL010000000
VERSION     DASYWL010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44019"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VGR22_05055"
     CDS             <1..423
                     /locus_tag="VGR22_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127970.1"
                     /translation="ATDPDTAHPQIGFADRQIKIGDLAIFGRRLEADAWRTAATPFKE
                     WYLEVRNGDGLITIVPEASALPLLTAIHKAEAVIILLDEDLDILPPTLSGTTIAAPAD
                     AAGFDEMDRDGDRPIRLFCDAPVSFALPGSGVTRPGAG"
     gene            complement(430..1263)
                     /locus_tag="VGR22_05060"
     CDS             complement(430..1263)
                     /locus_tag="VGR22_05060"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEV2127971.1"
                     /translation="MIESILLDILLVLVVVLLAAIGAYRGGLREAFSATGVVLGALLA
                     AEWHERWGEWLSRNTSLSDGGARFLVSVGLLIFATAVVGYGVGASFSYHPGPGGRMFG
                     AALGVAGALVATAYVLTWLRRDLFSGDEPEVVAESVLARLLDDSTGLILLVVSAGVIF
                     GAIFGSFVRERDGDVPEVSHTRSFRAMNAPDKVEPPRTANHSSAPVRVHQSRRWDGRA
                     GDMPATADRRWSNTWPSDAPGTRQEERASRQSEVQQARERRRSHHRGGSGRAEENGGS
                     R"
     gene            complement(1290..>2334)
                     /locus_tag="VGR22_05065"
     CDS             complement(1290..>2334)
                     /locus_tag="VGR22_05065"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEV2127972.1"
                     /translation="RPQMDDEVLIGLKGQESRVFRGEQSNTSIVFGDRVILKLFRKQR
                     EGINPEVEIGTFLTRYTSFEAFPKLYGTISLEDGERETTIGALQQFIPSVRDAWSWLT
                     ERVTDAALRPSTIEAAATLGSRTGEMHRALASGTDDAFVPEQASGAYATAVRAEAIAE
                     LRETVEQLEHRGVENAAALGDALASSLDAFLQIEGTMITRIHGDYHLGQVLRTEDRDF
                     AILDYEGEPTRSLVERRAKASPLRDVAGMLRSLDYAAETARRAGGGERDDLDAWYEDS
                     RDAFLRAYTDTVSSDEFLMRGWNAESRADVLAAFEVHKALYETRYELNNRPDWLEIPL
                     NALQRIAGIGNAR"
BASE COUNT          431 a          781 c          715 g          407 t
ORIGIN      
        1 gccacggatc ctgataccgc acatcctcaa atcggctttg ccgatcgcca gatcaagatc
       61 ggcgacctgg cgatttttgg ccggcggctt gaagccgacg cttggcgcac ggctgccaca
      121 ccattcaagg agtggtacct cgaggtccgc aacggagacg ggttgatcac catcgtgccg
      181 gaggccagtg cgctgccgct actcacagca atccacaaag cggaagcggt catcatcctg
      241 ttggatgagg atctggatat actcccaccg acactgtcag gcaccacgat cgcagcgcca
      301 gcggacgccg caggtttcga tgaaatggac agggacggtg accgcccgat tcggctcttc
      361 tgcgacgcac cggtaagctt cgcgctcccg ggttccggag tcacgcgtcc tggcgctgga
      421 tgaggtcgct cagcggctgc cgccgttttc ctcggcgcgc ccagatcctc cccggtgatg
      481 tgagcggcga cgttcccgag cctgctgcac ctcgctttgg cgagacgccc gctcctcctg
      541 tcgggtgccg ggcgcgtcgc ttggccaagt gttggaccac cgacgatctg cggtcgcggg
      601 catgtctccc gcccgaccgt cccaacggcg cgattgatgc acgcggaccg gcgccgagga
      661 gtggttggcg gtccgcggag gctccacctt gtcaggcgcg ttcatcgccc tgaatgatcg
      721 ggtgtgagag acctccggaa cgtcaccatc cctctcgcgc acaaacgacc cgaagatcgc
      781 cccgaaaatc acgcccgcgc tcacgaccag caggatcagg cctgtactat cgtcgagaag
      841 gcgagccagc acgctctctg cgacgacttc aggctcgtcg ccactgaaca ggtcccgccg
      901 aagccaggta agcacatacg ccgtcgccac cagtgcaccg gccaccccaa gcgctgcgcc
      961 gaacatgcgg ccgcccgggc cgggatggta gctgaacgag gctccgacgc cgtagccgac
     1021 aacggcggtc gcgaagatga gcagcccgac ggaaacgaga aaacgcgctc ccccatcgga
     1081 gaggctggtg ttccgagaga gccactcgcc ccaccgctcg tgccattcag cagcgagcag
     1141 cgcgccgagc acgaccccgg tagccgagaa cgcctcgcgc aggccgccgc gatacgctcc
     1201 gattgcggcc agcagcacga cgacgagtac cagcaggatg tcaagaagga tggactcgat
     1261 catcggagcg tctcaatcac gggaggatgt caccgagcat ttccaatacc ggcgatgcgc
     1321 tggagcgcat tcagggggat ttccagccag tctggccgat tgttcaactc gtaccgggtc
     1381 tcgtacagcg ccttgtggac ttcgaaggca gcaagaacgt cggcgcgcga ctccgcgttc
     1441 catcctcgca tcaggaactc gtcactcgag accgtgtcgg tgtaggcccg caaaaacgcg
     1501 tctctggaat cctcgtacca cgcgtcgagg tcatcccgct caccgcctcc ggcccggcgt
     1561 gcggtctcgg cagcatagtc caatgagcga agcatgcccg ctacgtcgcg aagcggtgac
     1621 gccttcgctc gccgctcgac cagagatcgc gttggctcgc cttcatagtc aaggatggcg
     1681 aagtcgcggt cttccgtccg caatacctgt ccaagatgat aatcgccatg tatccgagtg
     1741 atcatcgtgc cttcgatctg caggaacgcg tctagcgacg acgcgagcgc atcgccgagg
     1801 gctgctgcgt tttcgacgcc gcggtgctcg agttgctcga ccgtttctcg caattcggca
     1861 atcgcttcgg cgcgcaccgc ggtggcgtag gcgccactcg cctgctccgg cacgaaggcg
     1921 tcgtcggttc cggaggccag cgcgcggtgc atttcgccgg tgcgcgaccc gagcgttgcg
     1981 gcggcctcga tggtcgacgg tcgcaaggca gcgtcagtca ctcgctcggt gagccacgac
     2041 cacgcgtcgc ggacgctcgg gatgaactgt tgaagcgcac cgattgtcgt ttctcgctcc
     2101 ccatcctcca gtgagatggt gccatagagc ttgggaaaag cctcgaatga ggtgtaccgc
     2161 gtcaggaagg tgccgatctc gacctccggg ttgataccct cgcgctgctt gcggaagagc
     2221 ttgaggataa cccgatcgcc gaacacaatc gacgtattcg actgctcgcc tcgaaagacg
     2281 cgggactcct gccccttcag tccgatcagc acctcatcgt ccatttgcgg tcgg
//
LOCUS       DASYWL010000145         8251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_306956, whole genome shotgun sequence.
ACCESSION   DASYWL010000145 DASYWL010000000
VERSION     DASYWL010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8251
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306956"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..1270
                     /locus_tag="VGR22_05070"
     CDS             200..1270
                     /locus_tag="VGR22_05070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2127973.1"
                     /translation="MAESDVSIRRLYKRFGNLVAVDHVDLEVPSGAFVTFLGPSGCGK
                     TTTLRMIGGFEHPDSGQILIKGEEMGNRPPHRRDTSMVFQNYALFPHMSVADNIGFGL
                     RERKVPKSEIRERVQRMLELVELTGRGDRKPRQLSGGQQQRVALARSLVIEPTVLLLD
                     EPLGALDLQLRKQMQIELKRIQQQVGITFIYVTHDQEEALTMSDRIVVMNDGRVEQEG
                     DAEEIFENPRTRFVAEFMGAENVLDADVADRDGSSARLRIGTRELTLPKALPDGARTA
                     AVVVRPEKVRMDVPDGWRGTVTDRVYKGATMSYLIRLDDGTIVNADVAHDDPAHRHDV
                     GDEMMIAFRPEDIVVIPDTKGA"
     gene            1274..2173
                     /locus_tag="VGR22_05075"
     CDS             1274..2173
                     /locus_tag="VGR22_05075"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2127974.1"
                     /translation="MATTTTAQAATPARQARNFPTAQVISLGLPTLVVLLFFVLPIVL
                     IGIYSFYSINPETGLMQQDFTWANYERALTSPIYRNIFWRTLGIAALATGIALLLAYP
                     IGYFIGAVVQPKYQGPLLLLVLIPFWTSYIIRTYAWIGILQSNGFLDSVLGWVPGLPT
                     ELLYSRAAVIIGFVHVFLPIMILPIYASARNLDKRYIEAAYDLGASPVKAFLRVVLPL
                     TMPGVVAGIVLFFIPTFGSFVTPQFLGGTGEQMVGNIISEQFKEAFNWPFGAALSIIM
                     TLVVVVAVTLFFRFADVEEIYGS"
     gene            2163..2972
                     /locus_tag="VGR22_05080"
     CDS             2163..2972
                     /locus_tag="VGR22_05080"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2127975.1"
                     /translation="MGAELRGGGMLLRIYTGLVYLFLFAPILVVALTSFNPSGRATIP
                     NGLTTQWYSNLQEDERLIDALWTSLQIAVFTALIASLLGLMAAYGLSHFRFPGRGALQ
                     GFFYLPMLVPTVVSGIALLTWFGEIDLDTGFLTILIAHVIFALPFTLTIILTSFAGFD
                     TRLEEAAQDLGATPFRAFRYITLPLVMPGVLGGALMAFTLSFDEFVLTFFVAGGGVQT
                     LPLEIYNRIRYLLSPEINAIATIVMGFSIGVLLIGQIVVALRSRRAAEEPT"
     gene            2974..3450
                     /locus_tag="VGR22_05085"
     CDS             2974..3450
                     /locus_tag="VGR22_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEV2127976.1"
                     /translation="MAEVLEWLLRQALDGREDVRVATAESCTGGTIASRITSQPGSSA
                     YFVGGVISYANDVKERVLGVPVGVLANPGAVSEPCAWAMAEGIRDLLGATHAISTTGI
                     AGPEGGTARKPVGTVYIGITTPEGTRVEHHVFPGDRQAVIVAASDRALEMLLEEMP"
     gene            3626..5002
                     /locus_tag="VGR22_05090"
     CDS             3626..5002
                     /locus_tag="VGR22_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEV2127977.1"
                     /translation="MISPLSPRLASRAINVITSGVPEGLTTETGPGDSPLYHKTTLTN
                     GVRVVTGPMSGVHSTSLIFYYNVGSRYERPEVAGVSHYLEHMLFKGTERRPDPVMISE
                     EIEQVGGMINAGTGRESTTYWCKVPSTHTAMAFDVMADMLRNSLFSTTEMDKERKVIF
                     EEIRAVQDIPEELVHDLIDEVVWGDDPLGREIAGSIETVGSIDRDSMVDFWQRNYCPE
                     RLVIAAGGDVDHGEIVRMVEAAFGDLEQQTPDTTTLATIMQDAPRLRVVNRPTEQAHL
                     AISVPAVSYRDDRRHAQAMIDSILSSGMSSRLFQEIREKRGLVYSVFGYFRGYEDVGQ
                     GVVYAGTDIDRVEEATDAILGELKKLRDVRVSEEEITRNKELRKGRLLMSLEDSRAVA
                     SWVGSQEATYGEIKSPEQVMEEIDAVTADDVQALAHELFRDDRLNMVLIGPYDDSALF
                     EHHLTFGR"
     gene            5012..5518
                     /locus_tag="VGR22_05095"
     CDS             5012..5518
                     /locus_tag="VGR22_05095"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2127978.1"
                     /translation="MSDSRATPPLGTAQQFDVRVMGPNHRHLAAMILADATPEGTEEA
                     ALRVIEQHEATEGSAIFIAYLSGMPIAAFILTPAQMSIEMPLIAVRKEIRGRGIGKVI
                     ALDAVRRAGNRPVVTETPESALPFFTAIGFKKFGRRKGPNGELRWRVGWHTPGMRTPT
                     GQEQPSNT"
     gene            5649..6530
                     /gene="nadC"
                     /locus_tag="VGR22_05100"
     CDS             5649..6530
                     /gene="nadC"
                     /locus_tag="VGR22_05100"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEV2127979.1"
                     /translation="MTIPRDLPAATSGWRDLIDLALAEDVGNGDITTLSTVAEDATAT
                     GTLLAKEAGVLSGVDVAEAVFAIVDPAITFKAVKRNGDPIEVGDTIARLAGSARSLLT
                     AERTALNIMQRLSGVATATARYVDATKGTGATIVDTRKTTPGMRMLEKRAVLHGGGGN
                     HRFGLADGVLIKDNHLAAIGGDHPVRDAVRSARKLAPHTMKIEVEVTTLAELDKALEA
                     GADIVLLDNMDTTTMTEAARRRDVAAPGTLLEASGGITLERIAVIAATGIDLISVGAL
                     THSSKALDISLEFALSR"
     gene            6973..8196
                     /locus_tag="VGR22_05105"
     CDS             6973..8196
                     /locus_tag="VGR22_05105"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEV2127980.1"
                     /translation="MPVLATDRGGGRVQHTSPVRYALIATALGLFFALLAPVAMVTNQ
                     EAGAQEWSAPRTVYLPETGHTLDQVFLDYWREAGGAAAFGYPITPEITREDGSIVQYL
                     QYARFEYYPQGNEDGFYFALGNLGEELRPFNLQRSVASFTTTGAPEQPGSMTMDAGFL
                     GAWMPAEAPQSDSPYVRYVEETGHTVRYAFLSFWDQSGGVDYLGNPLTEEYVIDGVTY
                     QIFKRGQVAWENGKDAWLVPVGELLAEKYWLDTTPVAQGSIPTYSEELFIPPPTPTPE
                     PQIRQAPADGERWIEINLSTQYLIAWQGDVPVLETYVSTGKPGFDTPPGTYHILVKKP
                     SEDMEGVIGGEYYNVPEVPDVMYFTDVGHAIHGAYWHSNFGAVMSHGCVNVPLGTSNV
                     LYNWASIGTRVVIHW"
BASE COUNT         1480 a         2753 c         2628 g         1390 t
ORIGIN      
        1 cttctggacg gtgcgggagg gcaggatcat gcgaggcacc gagtactgga tctcgctcgg
       61 gcaggatgat gttcccgact ggcgccagcg gttcaccgtt gcgatcccgg acgcctaacc
      121 accttcaccg cctcccgatg ccgggttgcg gggtaaaatg gcgcaagaaa atcgacacgc
      181 gtgaggggga ccagatgtaa tggccgaatc cgacgtcagt atccgcaggc tctacaagcg
      241 attcggcaat ctcgtcgcag tcgaccacgt cgacctcgag gtgcccagcg gcgcgttcgt
      301 gacctttctc ggcccctccg gctgcggcaa gaccaccacc ctgcgcatga tcggcgggtt
      361 cgagcacccg gacagcggcc agatcctcat caagggcgag gagatgggca accgcccgcc
      421 ccaccggcgc gacacgtcta tggtctttca gaactacgcg ctcttccccc acatgagcgt
      481 agccgacaac atcggcttcg gtctgcgtga gcgcaaggtc ccgaagagcg agatccggga
      541 gcgcgtgcag cgcatgctgg agctcgtgga gctcaccggg cgcggcgacc gcaagccgcg
      601 ccagctctcc gggggccagc agcagcgcgt tgccctcgcc cgctcgcttg tgatcgagcc
      661 gacggtgctc ctgctcgacg agccgctggg ggcgctcgac ctccagctgc gcaagcagat
      721 gcagatcgag ctcaagcgca tccagcagca ggtcggcatc accttcatct atgtcaccca
      781 cgatcaggaa gaggcgctta ccatgtctga ccggatcgtg gtgatgaacg acggccgcgt
      841 tgagcaggag ggggacgccg aggagatctt cgagaacccg cgcacccgat tcgtcgccga
      901 gttcatgggg gcggagaatg tcctcgacgc cgacgtcgcc gaccgcgacg gcagttccgc
      961 ccgcctgcgc atcggcacgc gggaactgac gctcccgaag gcgctccccg acggtgcccg
     1021 caccgcggcg gtcgtcgtgc gaccggagaa ggtccgcatg gatgttcccg atggctggcg
     1081 aggcaccgtg acggaccgcg tctacaaggg tgcgacgatg tcctacctga tccggctgga
     1141 cgacggaacc atcgtcaacg ccgacgtcgc ccatgacgac ccggcgcacc gccacgatgt
     1201 cggcgacgag atgatgatcg cctttcggcc cgaggacatc gtggtgatcc ccgacacgaa
     1261 gggagcgtag ctcgtggcca ccaccacaac cgcacaggcc gccaccccgg cgcgacaggc
     1321 acgaaacttc ccgacagcac aggtgatctc gctcggcctg ccgacgctgg tggtgctgct
     1381 cttctttgtg ctgccgatcg tgctgatcgg gatctacagt ttctactcga tcaaccctga
     1441 aacaggcttg atgcagcagg attttacctg ggcgaactac gagcgtgcgc tcacctcccc
     1501 catctaccgc aacatcttct ggcgcacact cggcattgcc gcactcgcga ccggcatcgc
     1561 gctgctgctc gcctatccga tcggctactt catcggcgcg gtggtgcagc cgaagtacca
     1621 ggggccgctg ctgctgctgg tgctgatccc gttctggacc agctacatca tccgcacgta
     1681 tgcctggatc ggcatcctgc agagcaacgg ctttctcgac agcgtactag gctgggtgcc
     1741 cggattgccc acggagctgc tctacagccg cgcggcggtc atcatcggct tcgtgcacgt
     1801 cttcctgcca atcatgatcc tgccgatcta tgcctcggcc cgcaatctgg acaagcggta
     1861 catcgaggcc gcctacgacc tcggcgcgtc gccggtcaag gcattcctcc gggtggtgct
     1921 gccactgacg atgccgggtg tcgtcgccgg gatcgtgctc ttcttcatcc ccaccttcgg
     1981 ctcgttcgtg acaccgcagt tcctcggcgg caccggtgaa cagatggtgg ggaacatcat
     2041 tagcgagcag ttcaaggaag cgttcaactg gcccttcggc gcggcgctgt caatcatcat
     2101 gacgctcgtc gtcgtcgttg ccgtgacgct cttcttccgg ttcgctgatg tggaggagat
     2161 ctatgggagc tgagctgcgg ggcggcggca tgctgctcag gatctacacc ggcctggtct
     2221 atctcttcct cttcgcgccg atcctcgtcg tcgcgctgac ctcgttcaac ccctccggcc
     2281 gcgcaaccat cccgaacgga ctgacgaccc agtggtacag caacctgcag gaggatgagc
     2341 ggctcatcga cgcgctctgg acctcgctgc agatcgccgt gttcaccgcg ctgatcgcct
     2401 cgctgctcgg gttgatggcc gcctacgggc tcagccactt ccggttcccg ggacgcgggg
     2461 cgctgcaggg attcttctac ctgccgatgc tggtgccgac tgtggtgagt ggtatcgcac
     2521 tgctcacctg gttcggagaa atcgacctcg acaccgggtt cctgaccatc ctgatcgcgc
     2581 atgtcatctt cgcgctgccg ttcacgctga cgatcatcct caccagcttc gcggggttcg
     2641 acacccggct ggaggaagcc gcgcaggatc tcggtgcgac gccgttccgg gccttccggt
     2701 acatcaccct gccgctggtg atgcccggcg tgctcggcgg ggcgctgatg gccttcacgc
     2761 tctcgttcga cgagttcgtg ctgaccttct tcgtcgccgg tggcggcgtc cagaccctgc
     2821 cgctggagat ctacaacagg atccgctacc tgctctcgcc ggagatcaac gcgatcgcga
     2881 cgatcgtgat ggggttctcg atcggcgtgc tgctcatcgg ccagatcgtc gtggccctcc
     2941 ggtcccgcag ggccgccgaa gaaccgacct gacgtggcag aagtactgga atggctcctc
     3001 cgccaagcac tcgacggccg cgaggatgtc cgtgtcgcaa cggcggaatc ctgcacgggt
     3061 gggaccatcg catcgcgtat caccagccag cccggcagct ccgcctactt cgtcggcggc
     3121 gtcatctcgt acgcgaacga cgtcaaggag cgggtgctgg gagtccctgt cggcgttctg
     3181 gccaaccccg gggcggtcag cgagccgtgc gcgtgggcga tggccgaggg catccgtgac
     3241 ctgctgggcg cgacgcatgc gatctcaacc accggcatcg ccgggccgga gggaggcact
     3301 gcccgcaagc cggtcggcac ggtgtacatc ggcatcacga ctccagaggg aacgcgggtc
     3361 gaacaccacg tcttccccgg cgaccgccag gcggtcatcg tcgccgccag cgaccgcgcg
     3421 ctggagatgt tgctcgaaga gatgccctaa aatgctcatg cggccgacca tccagccgtg
     3481 atgtcgtagg ggaggacctt gtgtcctcct gttcatcgac gtgggaggac acaaggtcct
     3541 cccctacaat cacctgtggc cataatgcgg caatgcccgt accattccct gcactggaat
     3601 catctgcttc gaaggagttc ccttcttgat ttcgcccctt tcgccacgcc tcgcaagccg
     3661 cgctataaac gtcatcacct ctggcgtccc ggagggcctc accaccgaaa ccggaccggg
     3721 cgactccccg ctctaccaca agaccaccct caccaacggc gtccgggtcg tgaccggccc
     3781 gatgtccggc gtgcactcga cgagcctgat cttctactac aacgtcggct cccgctacga
     3841 gcgtccggag gtcgctgggg tctcccacta tcttgagcac atgctcttca agggcacgga
     3901 gcggcgcccc gacccggtga tgatctcgga ggagatcgag caggtcggcg gcatgatcaa
     3961 cgccggcacc ggtcgggaat caaccaccta ctggtgcaag gtgccgagca cccatacggc
     4021 gatggccttt gacgtgatgg cggacatgct gcgcaactcg ctgttcagca ccaccgagat
     4081 ggacaaggag cgcaaagtca tcttcgagga gatccgcgcg gtgcaggata tccccgagga
     4141 gctggtccac gatctcatcg atgaggtcgt ctggggcgac gacccgctcg gccgcgaaat
     4201 cgccggatcg atcgagacgg tcggcagcat cgaccgtgac tccatggtcg acttctggca
     4261 gcgcaactac tgcccggagc gcctcgtgat cgctgcggga ggcgatgtcg accacggcga
     4321 gatcgtccgg atggtcgagg cggccttcgg cgaccttgag cagcagacgc ccgataccac
     4381 gacgctcgcg accatcatgc aggacgcgcc gcggctacgg gtcgtcaacc gccccacgga
     4441 gcaggcacac ctcgccatca gcgttccggc ggtctcctac cgcgatgacc ggcgccacgc
     4501 gcaggcgatg atcgactcga tcctctcttc cggcatgagc tcgcgcctct ttcaggagat
     4561 ccgcgagaag cgcggcctcg tttactccgt cttcggctac ttccggggct acgaggatgt
     4621 cgggcaaggc gtcgtctacg ccgggaccga catcgaccgg gtcgaggagg cgaccgacgc
     4681 catcctcggg gagttgaaga agctgcggga cgtccgcgtc agcgaggagg aaatcacgcg
     4741 caacaaggag ctgcgcaagg gccgcctgct gatgagcctg gaggatagcc gtgcggtggc
     4801 atcgtgggtc ggaagccagg aggcgaccta cggagagatc aagtcacccg agcaggtgat
     4861 ggaggagatc gacgccgtta ccgccgatga cgttcaggcg ctggcgcacg agctgttccg
     4921 ggatgaccgg ctcaacatgg tgctgatcgg tccgtacgac gattcggcac tgttcgaaca
     4981 ccacctcacc ttcggaaggt agatgcaccg catgtccgat tcccgcgcga ccccaccgct
     5041 cggaaccgcc cagcagttcg acgtccgggt gatgggcccc aaccaccgcc atctggcggc
     5101 gatgatcctc gccgacgcga cgcccgaggg aaccgaggag gccgcgctcc gggtcatcga
     5161 gcagcacgag gcgaccgagg gcagtgccat cttcatcgcc tacctgagcg gcatgccgat
     5221 cgcggcattc atcctgaccc cggcgcagat gtcgatcgag atgccgctaa tcgccgtccg
     5281 gaaggagatt cgcggacggg gcatcggcaa ggtcattgcg ctcgatgccg ttcgccgcgc
     5341 cgggaaccga ccagtggtca ccgagacgcc ggagtcggcc ttgccgttct tcacggcgat
     5401 cgggttcaag aagttcggcc gccgcaaggg tccgaacggc gagcttcgct ggcgcgtcgg
     5461 atggcacaca ccgggtatgc gcacgccaac ggggcaggag caaccctcca acacgtaggg
     5521 gtcgacccgg tgagcacgat acgcgcgcag taccaccgtg accaccatgt ggatcgccgc
     5581 cctatgggtg gccgggcgga aaggtccgcc ctacggcttg acacgatcga aggtggagga
     5641 taccgcccat gacgatcccc cgcgatctgc ccgcagcgac ctccggctgg cgcgacctca
     5701 tcgacctcgc gctcgccgaa gacgtcggca acggtgacat caccaccctc tcgaccgtgg
     5761 cggaggatgc caccgcgacc ggtaccctcc tcgccaagga agccggggtg ctctccggtg
     5821 tcgacgtcgc cgaggcggtc ttcgcgatcg tcgatccggc gatcaccttc aaggcggtga
     5881 agcgcaacgg cgaccctatc gaggtcggcg acactatcgc ccggctcgcc ggatcggcgc
     5941 ggtcactgct gaccgccgaa cggaccgcgc tcaacatcat gcaacggctc tcaggcgtcg
     6001 ccaccgcgac cgcgcgctac gtcgacgcca ccaagggcac cggcgcgacc atcgttgaca
     6061 cacgcaagac cacccccggc atgcgcatgc tggagaagcg cgccgtgctg cacggcggag
     6121 gcggcaacca ccgcttcggt ctggctgatg gcgtgctgat caaggacaac caccttgccg
     6181 cgatcggcgg ggaccatccc gtgcgggatg ccgtgcgttc agcacgaaag ctcgcgccgc
     6241 acacgatgaa gatcgaggtc gaggtcacaa ccctggccga gctggacaag gcgctggagg
     6301 ccggtgcgga catcgtgctg ctcgacaaca tggacaccac cacgatgacc gaggcggcgc
     6361 ggcgacggga tgtggctgcg cccggcacgc tgctggaagc gtccggtggc atcacgctgg
     6421 agcgcatcgc ggtgatcgcc gcgaccggta tcgacctgat ctcggtcgga gcgctcaccc
     6481 actcctccaa ggcgctcgac atcagcctgg agttcgccct gagccgctga cccctcatct
     6541 cgctggcgcg ctaccactac agtcgcctca ctgcaaggtg ccccgcctgg gcgctttttc
     6601 ggcgtcattg acctgtccga gccaagtccc gcgatggcgc gaaaaccagc ccggcgttgg
     6661 cgacgtagtt cgtggtaggg taagcgcggc tcggatggta ggtcgtgccg gctgggacgg
     6721 caccacggtc aagctcaccc tctatacaat ccgcacggga caggatacta tgtgtacgta
     6781 caccttgatc gtgagggtga cgctgagcag tgacatggcg cactggcgcc gtcgggcggg
     6841 agcgagccga aaccgcagcg gccagtggga cgttttagcc agtgaggaac cggagccgag
     6901 ccggcagccg gccacggcgg gctgacaatc acacggtgga ggaagggttt ggtggtgagg
     6961 agattttcaa cgatgcctgt tctcgcaacc gacaggggtg gcggtcgagt gcagcacacg
     7021 agtcctgtca ggtacgcgtt gatcgcgacg gcactgggac tcttctttgc gctactcgca
     7081 ccggtggcga tggtcacgaa tcaggaggcc ggcgctcagg aatggtcggc accgcggacg
     7141 gtctatctgc cggagaccgg ccacacgctc gatcaggtct tcctcgacta ctggcgcgag
     7201 gccggcggcg ctgccgcatt cggctatccc ataaccccgg agatcacccg ggaggatggc
     7261 agcatcgtcc agtacctgca gtacgcgcga ttcgagtact acccgcaggg caacgaggac
     7321 ggcttctact tcgcgctcgg caacctcggc gaggagttgc gaccgttcaa cctgcagcga
     7381 tccgtggcca gcttcaccac cactggcgcg ccggagcagc ccggctcgat gaccatggac
     7441 gccgggttcc tcggcgcgtg gatgccggcc gaggcgccac agagcgacag cccctacgtc
     7501 cgctacgttg aggagaccgg gcacacagtc cgctacgcgt tcctctcctt ctgggatcag
     7561 agcggcgggg tcgactacct tggcaatccg ctcaccgagg agtacgtgat cgacggcgtc
     7621 acctaccaga tcttcaagcg cggccaggtc gcctgggaga atggcaagga tgcctggctg
     7681 gtgccggtgg gtgagctgct cgccgaaaag tattggctcg acacgacgcc ggtcgcgcag
     7741 ggcagcatcc caacctacag cgaagagctc ttcatcccac cacccacgcc gacaccggag
     7801 ccgcagatca ggcaggcacc agccgatggc gagcgctgga tcgagatcaa cctgtcgaca
     7861 caatacctga tcgcgtggca gggtgacgtc ccggtgctcg aaacctatgt cagcaccggc
     7921 aagccgggat tcgacacccc accgggaacc taccatatcc tcgtcaagaa gccgtccgaa
     7981 gacatggagg gcgtgatcgg cggcgagtac tacaacgtac cggaggtgcc tgacgtgatg
     8041 tacttcaccg acgtcggcca cgcgatccac ggcgcgtact ggcacagcaa cttcggtgcg
     8101 gtgatgagcc atggctgcgt gaacgtgccg ctgggaacct cgaacgtgct ctacaactgg
     8161 gcatcgattg gcactcgcgt ggtgatccac tggtaggcgg gccacaagac agttgacctg
     8221 ttccggcgcc gggcggggag cgttactgct c
//
LOCUS       DASYWL010000146         6687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_119427, whole genome shotgun sequence.
ACCESSION   DASYWL010000146 DASYWL010000000
VERSION     DASYWL010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6687
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119427"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..386)
                     /gene="rpmE"
                     /locus_tag="VGR22_05110"
     CDS             complement(48..386)
                     /gene="rpmE"
                     /locus_tag="VGR22_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEV2127981.1"
                     /translation="MKPEIHPKYVDATVTCSCGNTFQTRSTQPAIRTDLCSVCHPFYT
                     GEQRIVDTAGQVERFNRRMEAAQGDARSKRQRRIDARRQAEAEREAAMTGEGQTDEQV
                     APAEETSEDA"
     gene            complement(420..743)
                     /gene="rpmA"
                     /locus_tag="VGR22_05115"
     CDS             complement(420..743)
                     /gene="rpmA"
                     /locus_tag="VGR22_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEV2127982.1"
                     /translation="MAHKKGMGSTRNGRDSESKRLGVKVADGQYIRNGGIVVRQRGTQ
                     FFVGNNVGIGKDHTIFSKIDGVVRFEPVTRNKRRISVYPESVYPVGGGQMAAAIETSD
                     TPAAD"
     gene            complement(782..1132)
                     /gene="rplU"
                     /locus_tag="VGR22_05120"
     CDS             complement(782..1132)
                     /gene="rplU"
                     /locus_tag="VGR22_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEV2127983.1"
                     /translation="MSEQQQTTATTSGPYAIIETGGKQYRVKEGDSITIERLATEAGE
                     DVTLDRVLMVGGNGSTKIGTPVVDGASVTAHVDEHFRGEKLVIFKYKPKKRYRRRTGH
                     RQELTRLTITGITG"
     gene            1528..3240
                     /gene="hemC"
                     /locus_tag="VGR22_05125"
     CDS             1528..3240
                     /gene="hemC"
                     /locus_tag="VGR22_05125"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEV2127984.1"
                     /translation="MLTVGTRGSALALHQANIVGELLAARHPQLDVQVRVITSQGDLD
                     KESPLTKIGGRGVFTSSLQRTLTLQQVDAAVHSSKDVPSLSAIGLALAAFPQREDARD
                     VVVSRHGMGLAGLPPNPVVGTSSRRRAVQVQALRPDAQIIELRGNIDTRLQKAMAEPY
                     DAVILAAAGLTRMGWIDRATELLPVNRFTPAPGQGALAVETREAPDPAFALVAQIDDP
                     SVRQALEIERAFLRGIGGGCTTPLGAHAVVETCHGQRIARFFGMLARDDGSGLVRVYD
                     EWPLERAVDAAFDTAGALVREVHPNRIFGAGTEEARQLKGMKVIVTGTDDLASRAATE
                     VERRGGDPVPLRTIQILPPADLESLQDALAELQRGGFDHLVLTSRQGVGAVSSILKGL
                     PAETVKIGAIGEATAAALRDLGIEPDVVAEDSRGEGMLAALERHVRPGERVLLPLSSR
                     ARRVLAEGLARRGADVTRVDAYETRLVTEPDPNVVSLVEKGEIDAVLLASPSAVRGFV
                     AQLGTLLPVLSGATFVAIGRMTADVMEEHGLPVHAVPSEPGAEAMVIALVAGLWGASI
                     DEGA"
     gene            3290..4228
                     /gene="hemB"
                     /locus_tag="VGR22_05130"
     CDS             3290..4228
                     /gene="hemB"
                     /locus_tag="VGR22_05130"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEV2127985.1"
                     /translation="MTPALRNFVREHHLKPSDLIAPLFVVEGEDVRNPIGSMPGQAQL
                     SIDLLVQEVGELLSRGVPGVLLFGIPDIKDAEATGAWDENGVVQRAVAAIKDRHPDAL
                     VITDVCACEYTDHGHCGILIDGEVDNDLTLPLLARTAVSHARAGADIVAPSDMMDGRV
                     CVIREALDGEGYTGTPILSYAAKFASAFYGPFREAAESTPAFGDRRPHQMDPGNAREA
                     MREIEIDIAEGADMIMVKPALPYLDIIRTARDRFDLPLSAYNVSGEYAMIKAAAERGW
                     IDEERVVMESLLSMKRAGAERIFTYHAKEVAEWLQR"
     gene            4213..5265
                     /gene="hemE"
                     /locus_tag="VGR22_05135"
     CDS             4213..5265
                     /gene="hemE"
                     /locus_tag="VGR22_05135"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEV2127986.1"
                     /translation="MAAEVAAMTGRERFLAALRREQPDCTPVWFMRQAGRCLPEYREL
                     RKKHSFLEVARTPELATEATLMPIDRFNVDGAVLFADIILPLEGMGVPFEIRPGVGPV
                     IEEPVRSTAAVDALRVVEPEEGTSYVLDALRMLKGELGQRAALLGFAGAPFTLACYLI
                     EGRPTKEYPITKGLMYGEPELWHRIMETLTEVTIRYLQGQARAGADVVQLFDSWLGLL
                     DEASFREYVQPYTARIFEAMGGVVPTVHFSTGTVQLLEAIGETGPGVVSVDWRLPIDV
                     AWEQLGHRTGIQGNLDPARLMASWDIIEQGADDILHRVHGRAGHVFNLGHGVNPATDP
                     DKLTRLVDHIHTASAR"
     gene            5943..6563
                     /locus_tag="VGR22_05140"
     CDS             5943..6563
                     /locus_tag="VGR22_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2127987.1"
                     /translation="MTPADSTRASGRGEILAVARRLFMTRGFRAVTTREIADAVGLTQ
                     PALYHHFGGKEALYVAVLKEDLARQSAAIRAVVETDAPARERLATLAANAAAQTDHDL
                     GQMFHDLRFEVSEATREHIGLAFREAMMQPMLGLVDALVSEGEIAPPEDAAMSHAEVA
                     MFVLSVIRMLKDTNAGPARGPRRSEREIGEMTVRLVLRGVGSHANP"
BASE COUNT         1066 a         2068 c         2285 g         1268 t
ORIGIN      
        1 atttccgggg ccgctgctcg atgttgtgtg ttgtgagcgg ggagtgacta ggcgtcctcg
       61 ctggtctcct cggccggggc gacctgctcg tcggtctgtc cctcgccggt catggcggcc
      121 tcgcgctcgg cctcggcctg gcggcgcgca tcgatgcggc gctggcgctt cgatcgggca
      181 tcgccctgcg cggcctccat gcggcggttg aaccgctcga cctgtcctgc tgtgtccacg
      241 atgcgctgct cgcccgtgta gaacggatgg cagacgctgc agaggtcggt gcggattgcc
      301 ggctgcgtgg agcgcgtctg gaacgtgttg ccacacgagc aggtcacggt cgcgtcaacg
      361 tactttgggt gaatttccgg cttcatgcag agtgttctcc gtggaaactg gggcgtcgac
      421 tagtctgcag ccggagtgtc ggacgtctcg atcgcagcag ccatttggcc gccaccaacc
      481 gggtagacgc tttccgggta gacactgatg cggcgcttgt tccgggtgac cggctcgaag
      541 cgcacgacgc cgtcgatctt gctgaaaatg gtgtggtcct tgccgatgcc gacattgttg
      601 ccgacaaaaa actgggtgcc acgctggcgg acgacgatcc cgccgttgcg gatgtactga
      661 ccgtccgcga ccttcacgcc gagacgcttg ctctcgctgt cacggccgtt gcgggtcgaa
      721 cccataccct tcttgtgagc catggtgaag ttcctttccg tgaatccggt ctcggcgtca
      781 attagccggt gatgccggtg atcgtcagcc gggtcagctc ctggcgatgg ccagtgcggc
      841 ggcggtagcg cttcttcggc ttgtacttga agatgaccag cttctcgccg cggaagtgct
      901 catccacgtg tgcggtgacg ctggcgccat cgaccaccgg cgtgccaatc ttcgtggagc
      961 cattgccccc gaccatcagc acacggtcca gggtgacgtc ctcaccggcc tcggtcgcga
     1021 ggcgctcgat ggtgatgctg tcgccttcct tgacgcggta ctgcttgccg ccggtctcga
     1081 tgatcgcgta gggaccgctc gtggtcgctg tcgtctgctg ttgttcactc atcgttactc
     1141 aacctgtcct gctgtgaacc ctgccgggcg acgaggcgct cctgtgcgtc agcccctcgc
     1201 gatcggaggg ggcaatgccg gccagcgtgc ccgtgctgat atcctgctga ttgcacacaa
     1261 atatgggtgc gtagggcacc cgagacatct cattgtatca caggtgctgc cggtctcgca
     1321 cctggtttgg tgcatgcagg cgccgcttca ttaagtatac ggtgtggttg acgcgcggat
     1381 tcggctctgc ctacactcat cgatggtatt ggcgggcatg actgtcaggc tcgctgcccc
     1441 ctcgacccgc gtttccccgg caaaggcctc cttgtggtca atttcgctcg gcatagctca
     1501 tccgaaaccc gcgacccgag caccggtgtg ctgaccgttg ggacccgcgg aagcgcgctg
     1561 gcgctgcatc aggcgaacat cgtcggcgaa ctgctcgcgg caaggcatcc gcagcttgac
     1621 gtgcaggtcc gggtgattac ctcccaagga gacctcgaca aggagtcgcc gctgacgaag
     1681 atcggcggcc gcggggtctt cacctcctcg ctgcagcgca ccctgacgct gcagcaggtc
     1741 gacgccgcgg tgcactcctc caaggatgtg ccgagcctct ccgcaatcgg attggcgctc
     1801 gccgcgtttc cgcaacgaga ggacgcgcgt gacgtggtgg tgtcgcggca cggcatgggg
     1861 ctggccggcc tgcctccgaa cccggtcgtc ggcacctcgt cccgacgccg ggcggtacag
     1921 gtgcaggcgc tgcgaccgga cgcgcagatc atcgagctgc gcggcaacat cgatacccgc
     1981 ctgcagaagg cgatggccga gccgtacgac gccgtcatcc tcgctgcggc cggcctcacc
     2041 cgcatgggct ggattgaccg tgcgacggag ctgctgccgg tgaaccgttt caccccggcg
     2101 ccaggacaag gtgccctcgc ggtcgagacc cgtgaggcgc cggatcccgc cttcgcgctg
     2161 gttgcccaga ttgacgatcc gagcgtgcgg caggcgctgg agatcgagcg ggccttcctg
     2221 cgcggcatcg gcggaggttg taccacgccg ctgggcgcgc acgccgtggt cgagacgtgc
     2281 catgggcagc gcatcgcgcg tttctttggc atgctggcgc gggacgatgg cagcgggctc
     2341 gtccgcgtct acgacgagtg gccgctcgag cgcgcggtgg atgccgcgtt cgacaccgcc
     2401 ggcgcgctgg tgcgcgaggt gcatccgaac cgcatcttcg gcgcagggac cgaggaggct
     2461 cgtcagctca agggcatgaa ggtgatcgtc accggtaccg acgatctcgc gagccgcgca
     2521 gcaacggagg tcgagcgccg gggcggcgat cctgtgccgc ttcgcacgat ccagatcttg
     2581 ccgcccgctg atctggagtc gctgcaggat gcgctggcag agctgcagcg cggcgggttc
     2641 gatcacctcg tgctgacgag ccggcagggt gtgggggccg tatcctcgat actgaagggt
     2701 cttccggccg agacggtgaa gatcggcgcg atcggcgagg cgactgccgc tgcgctgcgt
     2761 gacctcggta tcgaaccgga tgtggttgcc gaggactcgc gcggtgaagg gatgcttgcc
     2821 gcgctcgaac gacatgtccg gcccggcgaa cgtgtgctgt tgccgctctc gagccgcgcc
     2881 cggcgggtgc tggcggaggg gctggctcgg cgcggcgcgg atgtcactcg ggtggatgcc
     2941 tacgagaccc gcctggttac cgagccggac ccgaacgtgg tctcgctggt cgaaaagggc
     3001 gagatcgacg cggtgctcct ggcgtcgcca tcggcggtga gaggctttgt cgcccagctc
     3061 ggcacgttgc tgccggtgct gtccggggcg acattcgtgg ccatcggccg gatgacagcc
     3121 gatgtcatgg aggaacacgg cctgccggtc catgcggtgc cgtccgagcc cggcgccgag
     3181 gcgatggtca tcgcgctggt tgcgggcctg tggggagcga gcatagatga gggtgcctga
     3241 gacacgaggc aggacgttga acgacttcgc cagaacgcga aggacccgga tgacgccggc
     3301 cctgcgaaat ttcgtccgtg agcatcactt gaagccgtcg gacctgatcg cgccgctctt
     3361 cgtggtcgag ggcgaggacg tgcgcaaccc catcggctcg atgccggggc aggcgcagct
     3421 ctccatcgat ctcctcgtcc aggaggtcgg tgagctgctt tcgcgcgggg tgccgggcgt
     3481 gctgctcttt ggcatcccgg acatcaagga tgccgaggcg accggcgcct gggacgagaa
     3541 cggggtcgtg cagcgcgcgg ttgcggcgat caaggaccgc caccctgatg cgctcgtcat
     3601 taccgacgtc tgtgcctgcg agtacaccga tcatggccac tgcggaattc tgatcgacgg
     3661 cgaagtcgac aacgacctca cgctgccgct gctcgcccgg acggcggtga gtcatgcacg
     3721 ggcgggagcg gacatcgtcg cgccatcgga catgatggac gggcgagtgt gcgtgattcg
     3781 cgaggcgctc gatggtgagg ggtacacagg caccccgatt ctctcctatg cggcaaagtt
     3841 cgcctcggcc ttctacgggc cgttccgtga ggcggcggag agcactccag cgttcggtga
     3901 ccggcgtccc catcagatgg atccgggcaa cgcccgcgag gcgatgcgcg agatcgagat
     3961 cgacatcgcc gagggggcag acatgatcat ggtcaagccg gcactgccct atctcgacat
     4021 catccggacc gcgcgcgacc ggttcgacct gccgctctct gcgtacaacg tctccggcga
     4081 gtacgccatg atcaaggcgg ccgccgagcg cggctggatt gacgaggagc gggtcgtgat
     4141 ggagtcgctg ctctcgatga agcgcgctgg cgccgagcgc atctttacct atcatgcgaa
     4201 agaggtggcc gaatggctgc agaggtagcg gccatgacgg gccgggagcg cttcctggcg
     4261 gcgctccgtc gtgagcagcc ggattgcacg ccggtctggt tcatgcgcca ggccggccgc
     4321 tgtctgcctg agtatcgcga gctgcgcaaa aagcattcct tcctcgaggt cgctcgcacc
     4381 ccggagctgg ccaccgaggc gacgctgatg ccgatcgacc gcttcaacgt cgatggtgcg
     4441 gtgctcttcg ccgatatcat actgccgctg gagggcatgg gggtgccgtt tgagatccgg
     4501 cctggcgtcg gcccggtcat cgaggagccg gtccgcagca ccgcggcggt cgatgccctg
     4561 cgcgtcgtcg agccggagga ggggacatcg tatgtgctcg atgcgctgcg gatgttgaag
     4621 ggcgagctgg gccagcgggc agcgctgctc gggttcgctg gcgcgccctt cacgctggcc
     4681 tgctacctga tcgaggggcg accgaccaag gagtacccga tcaccaaggg gctcatgtac
     4741 ggcgagccag agctctggca ccggatcatg gagacactga ctgaggtcac catccgctac
     4801 ctccagggac aggctcgtgc cggcgccgac gtcgtgcagc tttttgactc gtggctcggg
     4861 ctgctcgacg aagcgtcgtt tcgcgaatac gtacaaccat atacggctcg gatcttcgag
     4921 gcgatggggg gtgtcgtgcc gacggtccac ttcagcaccg gcaccgtgca gctgctggag
     4981 gcgatcgggg aaaccggtcc gggcgtcgtc agcgtcgact ggcggctgcc gatcgatgtc
     5041 gcctgggagc agctcgggca tcgtaccggc attcagggca acctcgaccc cgctcgcctg
     5101 atggcatcgt gggacatcat cgagcagggc gccgacgaca tcctccatcg ggtccatggt
     5161 cgggctgggc acgtcttcaa cctcgggcac ggcgtcaacc cggccacaga tccggataag
     5221 ctcacgcgcc tcgtcgatca cattcatacc gccagcgccc ggtagtacat ttctggtcta
     5281 ctgcgatcga aacgcccgcc ccggtatatg ggacgggtgt tttcgctttt cggtccacga
     5341 gcatagctgc ttgtgacgtt cgaatgaatc atcatcgcct tgaacgccga gaggcgttcg
     5401 ggtcgacccc gccacaagat gcggggctac gcgcaggtca gctccttacg gaatgaccgg
     5461 tttctcgagt gattgcgcaa atagcttatt atggaaggat gccagcagaa aaatgtggca
     5521 gtgtggcagt gttctctgat ttgcccagag ttttgggcga tcaaccctgc caaggtgaac
     5581 gggcagcccg ggagattttc ataatgcaat gcggtgtgat gcgctggttt aagggactct
     5641 actgggtcaa atgcagcgaa gagggggaat tggcgcgcgc aggtggtgga acgtcaattc
     5701 tgggccaacg cgcgcatcgt ttctccgaca agggaacgtc agcgagcctg gagccgagga
     5761 ataccctgtc tgccggcatg gacgcatgtt gttgctccac tccgcgccct gtgggtgctt
     5821 cggtgggatc gacagatggg gtcgtgtctg cgagccgagg aatgaaccgg ttcgaacatg
     5881 gcttcattcc tcggctcgtc atcttgcctc gtgcccttat caggtgataa ggagcggtga
     5941 gcgtgacacc agcggattcg acacgggcat ctggtcgcgg cgagattctc gcggtggcga
     6001 ggcggctctt catgactcgc ggttttcgcg cggtgacgac ccgcgagatc gccgatgccg
     6061 tgggactgac ccaacccgcc ctctaccacc atttcggggg caaggaggcg ctctacgtcg
     6121 ctgtcctcaa ggaggatctg gcgcgccagt cggcagcgat ccgcgcagtg gtggagaccg
     6181 atgcaccggc gcgtgagcgg ctcgccacgc tggcggcgaa cgccgctgca caaacggatc
     6241 acgatcttgg gcaaatgttc cacgacctga gattcgaagt ctccgaggcg actcgggaac
     6301 atatcggcct ggcttttcgc gaggcgatga tgcagccgat gctcggtctg gttgatgcac
     6361 ttgtcagtga gggtgagata gcaccgcccg aggatgctgc catgtctcat gccgaagtcg
     6421 cgatgttcgt gctctccgtg atccgaatgt tgaaagacac caacgctggt ccggctcgcg
     6481 gtccgcggcg ctctgagcgc gagatcggcg agatgaccgt taggctggtg ctgcgcggcg
     6541 tcggctcaca tgccaatcca tgacgattcg agcccggtgg cctcatcagg agtagctgcc
     6601 aggtgctgag ccgcatcacc gacttcgcgt cttcgtccaa ggcaaatggc tcgttactgc
     6661 ggtctgggcg ctgctcgctg ccgtcat
//
LOCUS       DASYWL010000147        10719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_644968, whole genome shotgun sequence.
ACCESSION   DASYWL010000147 DASYWL010000000
VERSION     DASYWL010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10719
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644968"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VGR22_05145"
     CDS             <1..311
                     /locus_tag="VGR22_05145"
                     /inference="COORDINATES: protein motif:HMM:NF020772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HEV2127988.1"
                     /translation="VEANPDASGNVERALEQAARELLLLESSDWPFLVTPGQAKEYAT
                     KRFREHVARFEELAEMVESGNIDQTRLTELENRDNPFANIDYHDFAERQGKAEYPLAG
                     "
     gene            complement(398..1948)
                     /locus_tag="VGR22_05150"
     CDS             complement(398..1948)
                     /locus_tag="VGR22_05150"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2127989.1"
                     /translation="MNPHRAINWAPHWILLPYVWEGLLGFDAQGEVVPVLASAIEAEE
                     DGLVWVATIRPEASFVSGNPVTAEAMITGWKRALQGQRIAPMSPYMRRVEGFEDFVAG
                     ESEDIGFEARDERRVAIRLAEPYALFPEDLATFVWAAVDVTALAELPESQIPFSDASA
                     GRWRFVEISETGDIVMALRDDPTDAPSPFSRVTWRPFEGPLAAEEAVEALRENDIALA
                     DAPAPIHVAIEDEEQPAGNLEVIEPLGSTMLIGMDFGQEPFSDPRVRRAIGHAIDRKR
                     WATEIMGGEFVPAESITPPVIAETSGYSSPELLVFDPELARSLIREAGITEEAMPTIS
                     YFQPADTPEPEVEEAAALLAMIQENSGLIIEHNRSLTAEQIDARRQDEGGLQLDLRWW
                     WPLTNSPSGLADLGLPASTAMDGWFNWSGSLENADVAEAALAFEEIIDEAQTTMDQTE
                     RSELFASAETLLVETAVYIPLGHWIQAFLRSDQLQGTRHGAFTGYMPIAFDEEVEWTP
                     PGEGTPSG"
     gene            complement(2289..3332)
                     /locus_tag="VGR22_05155"
     CDS             complement(2289..3332)
                     /locus_tag="VGR22_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEV2127990.1"
                     /translation="MNFDLLERLCQVAGIGSRERRVRDLVREELKAHVDEQYVDALGN
                     LVSIRRGSGPRIMVAAHMDEIGFVVRHIDDNAFIRVQQVGGFDPRVLAAQRVRVHTRD
                     GQALAGALQPGTKPVHLTKAGESRELKLEDLFIDTGLSVDAVRALVSIGDMVTLDRPS
                     VRMGDLVVSKALDDRVGLYVMIEALRDVDETNAEIISVATTQEEVGLRGAQTAAFHLE
                     PDIAIALDVTIAGDIPGIPPEEAVTRLGAGTAIKVFDSSQLPHPTIIETLRSIAESER
                     IPHQLEVLPRGGTDAGAIQRTRGGAYTGTISIPCRYMHSANEAAHERDIDASIQLLAR
                     FLERADLEEIRYR"
     gene            complement(3452..5335)
                     /locus_tag="VGR22_05160"
     CDS             complement(3452..5335)
                     /locus_tag="VGR22_05160"
                     /inference="COORDINATES: protein motif:HMM:NF023260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HEV2127991.1"
                     /translation="MQSRTHTLTVGFSVAHWMMLLVAFALVSALIVPLAPASAQTFVT
                     NEPVDIVPETAYVYVEANLDTSTEQGALAADLLQRAGLADLLEEEAGTDEIPEGLHVA
                     FVLTSIPDLEALDEVGDVTTDPMAVTKSFDGGGFAVIVSSEGSEQFIADERARMEADT
                     GAGLGEITEAEHGGVTITAFEPSDAFAEGTAVATIGDYRVFAMRAEDLHPLIDTFNED
                     LTSLASSERYQLLTDLLPSEHVASGWINGVALLEAVEASSPEAIASVDVNTLAALDAW
                     TAFTFSAEPQGFRLETRSLPNELVESEPVALDGAFLDTVPADAVFVTTGTDIDSSGLV
                     TLLAFLFATELVGEDLMATPVDQVDVAQAQNDVFERSESLLGFNIKTDFIDNLTGRYG
                     IFVSVDDITAETPAIDALIVSDITNPVVVQDVMSKISFIAGAALGDQGSIETRDVDGS
                     QVYQIALSEDTAGIDSKIEFGVIDDEVVISVGSGLDDYLTGPAQPLSQDETFETLMAR
                     LPDEYNHLTYIDLHQVVTMATELSEGQDFALEDADPACAEFASQAEAQAAYDEDPFTN
                     FLLDLDFDGEACEDYPFAGATPSASPVVQEDRFANIHGLATVMYEEGDVEASSTFILI
                     DGE"
     gene            complement(5473..5601)
                     /locus_tag="VGR22_05165"
     CDS             complement(5473..5601)
                     /locus_tag="VGR22_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127992.1"
                     /translation="MEILAELLKVVATLGVFVVVALLTAALFIIILAMATGIDRNI"
     gene            complement(5763..8372)
                     /gene="clpB"
                     /locus_tag="VGR22_05170"
     CDS             complement(5763..8372)
                     /gene="clpB"
                     /locus_tag="VGR22_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEV2127993.1"
                     /translation="MATDQNFTEKARQAIIAGQQITQEKNLAQYEPEALLVGLVDQPD
                     GIVPQILQRLNLDPAVVLQEANALIDRAPKLQYSAEAVVSQALRKALQDAERQAQQFG
                     DEFISTEHLLLGILGGDRTQAASLLNRLGADRDRVLGALTEIRGNQRVTDENPENTYQ
                     ALEKYGRDLTELARDGKLDPVVGRDDEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIAE
                     GLAQRIVRGDVPEGLKDKRIISLDLGALVAGAKFRGEFEERLKAVLKEVQSSEGQVIL
                     FIDELHTVVGAGAAEGSMDASNMLKPMLARGELHAIGATTLDEYRKHIEKDAALERRF
                     QPVLVGEPTVEDTISILRGLRERYEVHHKVKILDSALVAAATLSDRYITERFLPDKAI
                     DLVDEAASRLRMEITSMPVELDEAERRIMQLEIEREALRKETDQASKQRLEALEEELA
                     NLKEERDALRSRWDQERQAIIAISELRDEMDHLRVEIEQAQRNYDYNRAAELQYGRAK
                     ELEQKLQEEEARLARLQSSGKLLKEEVDAEDIAEVVSRWTGVPISRLMEGEIEKLVHM
                     EDRLHARVIGQDEAIEAVSNAIRRSRAGLSDPNRPMGSFIFLGPTGVGKTELARALAE
                     FLFDSDQAMVRIDMSEYMEKHAVSRLIGAPPGYVGYDQGGQLTEAIRRRPYSVILFDE
                     IEKAHPDVFNILLQVLDDGRLTDGQGRVVNFTNTVVIMTSNLGSQAIMQMNESNAGEI
                     RAKVMDALRANFRPEFLNRIDETIIFTQLSREEIAEIVELQLDQIRSRLGDRNMDLQV
                     TEEARDLLATLGYDPAFGARPLKRVIQKRVLDPLALALLSGALHEGETITLGVEDNDF
                     ILRGQLTEAPVVA"
     gene            complement(8431..8805)
                     /locus_tag="VGR22_05175"
     CDS             complement(8431..8805)
                     /locus_tag="VGR22_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV2127994.1"
                     /translation="MASRYSEPLFVISVAARLVEMHPQTLRKYEREGLIAPSRTAGNL
                     RLYSDEDIERLRQVKYLVETRGLNLAGVQLALELTQSLRKLRASMGRSPDSRAWTDDL
                     DRILVELGFVEQQQVRENARRR"
     gene            9034..9678
                     /locus_tag="VGR22_05180"
     CDS             9034..9678
                     /locus_tag="VGR22_05180"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEV2127995.1"
                     /translation="MANLITVARLILLFVVIWLIYVGNVQVVTASMMLIIIVFASDGL
                     DGWVARKRGSTSTFGAVFDILGDRIVENALWVIFSDLDLIPVWIPLMVLTRGFIVDGV
                     RSMSYAEGKTPFGKNNMMKSAFTEWLTAGRFMRSFFGWAKAAGFVFLTGLAGKERLDT
                     GGTFLGTLYDQRWYLWLGWGLVWAAVILTIVRGVPVIVDAWAHIQGDAEQPLSA"
     gene            9713..>10719
                     /locus_tag="VGR22_05185"
     CDS             9713..>10719
                     /locus_tag="VGR22_05185"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEV2127996.1"
                     /translation="MPNDRGTVRQAVREFRQEERQEFRYLLSVTLAIGFSWVFWLIPG
                     PIRGWIADHSGDLFYRISNTYPDNVRDNVQTVLDYIGDARMDPEPLVPSIFRTSARNF
                     MDLIITPRQPARSFLTSVRLVSGSWQTLDRVLEAGKGGILVSGHVGSIDYIGQVLTVR
                     GYKLTVVTGRTTSRFIFDGVSWLRASRGATLVEPTPSGIRKVMKAVRRNEFAVFVTDR
                     DFFQNGQDVVFMGRQTTLPPGPVRIARETGAPLIPVFTRRVARGHELTISEPFHIERT
                     SDVRADMAVGMERLVEVLEAGIISSLDQWVMFQRVWLEPQRVPVRVFPVGSPLESELL
                     ER"
BASE COUNT         1894 a         3429 c         3373 g         2023 t
ORIGIN      
        1 tcgtcgaagc caacccggat gcaagcggca acgtggagcg cgcacttgag caggccgcgc
       61 gggagttgct cttgctggag agcagcgact ggccgttcct ggtcacgccg ggccaggcga
      121 aggaatacgc gaccaagcgc ttccgggagc atgtcgcacg attcgaggaa ctggccgaga
      181 tggtcgaatc gggcaatatc gaccagacac ggctcacgga gctcgaaaac cgcgacaacc
      241 cgttcgcgaa catcgactac cacgacttcg ccgagcgaca gggcaaggcc gaatacccgc
      301 tcgccgggta aagctttcaa gcccagcagc gagagcccca gacagcccat cccgagcacc
      361 ggaaggggtg tcgttcactc gcctcgcgat agacgcacta tccacttggg gtgccctcgc
      421 caggcggagt ccactcaact tcttcgtcga acgcaatcgg catgtaaccg gtgaaggccc
      481 cgtggcgtgt gccctggagc tgatccgagc gaaggaacgc ctggatccag tggccgagcg
      541 ggatgtacac ggcggtctcg acaagcagag tctccgcact tgcaaacagt tcgcttcgct
      601 ctgtctgatc catagtggtc tgcgcctcgt cgatgatctc ctcgaacgca agcgccgctt
      661 cggccacgtc agcgttttcc aggcttccgg accagttgaa ccagccatcc atcgctgttg
      721 aggccggcag cccgagatcc gcaagcccgg atggcgagtt ggtgagcggc caccaccagc
      781 ggaggtcaag ctgcagccca ccttcgtcct ggcgacgagc atcgatctgt tcggccgtga
      841 gcgatcgatt gtgctcgatg atcaagccgc tgttctcctg gatcatggcc agcagcgccg
      901 cagcttcctc gacctccggt tcgggtgtgt ccgccggctg gaagtaggag atggtcggca
      961 tcgcctcctc ggtgatgccg gcttcccgga tgagagatcg ggccagctcc gggtcgaaca
     1021 ccagcagctc cggggacgag tagccagacg tctcggcaat taccggcgga gtaatcgact
     1081 cagccggaac gaactcaccg cccatgatct ctgtcgccca gcgcttgcgg tcgatcgcat
     1141 gtccgatggc gcgtcgcacc cgggggtcac tgaacggctc ctgaccgaag tccatgccga
     1201 taagcatcgt ggaccccagc ggttcgatca cctccaggtt gccggccggc tgctcctcgt
     1261 cttcaatcgc cacatggatg ggggccggag catcggccag cgcgatgtcg ttttctcgca
     1321 gcgcctcgac cgcttcctcg gccgcgagcg gaccctcaaa gggacgccaa gtcacgcggc
     1381 tgaacgggga aggggcgtcg gtgggatcgt ccctaagcgc catcacgatg tcgccggtct
     1441 cggaaatctc cacgaagcgc cagcgccccg ccgaagcatc gctgaacgga atctgcgatt
     1501 ccggcagctc ggcaagtgcc gtcacgtcga ccgctgccca cacgaaggtc gcaagatcct
     1561 cggggaacag tgcatagggc tcggcaagcc ggatggccac cctacgctcg tcgcgcgctt
     1621 cgaatccaat gtcctcgctc tcgccagcga cgaagtcctc gaatccttcg acccgacgca
     1681 tgtagggcga catcggcgcg atgcgctgcc cctgcagggc ccgtttccac ccggtgatca
     1741 tcgcctcggc tgtgaccgga ttgcccgaaa cgaagctcgc ttctggccgg atcgtcgcga
     1801 cccacaccag cccgtcctcc tctgcctcga tcgcggaggc gagcaccggc acaacttcac
     1861 cctgggcgtc gaagccaagc agtccctccc agacgtaggg cagaaggatc cagtggggtg
     1921 cccagttgat cgcccgatgg gggttcaagg agagtacctg gccgtgtggg tagagcggca
     1981 ggaccggtcc agtcgcatcg gcgttccctg gcgtggactg agccgtcagt gaggacggga
     2041 ccgccgctgt tgcgagcacg gccgcaccgg cagatgcccg cataagaccg cggcgcgaga
     2101 ggcggacccg cgtacctccg ttggcagggc tgtttgtgat gtgagcgtcg ttctgcatgc
     2161 gcttcctccg attgcgtcgg accgtccagc ggcgtatggc gacccgccgg agctcttacc
     2221 ccttcgaacg catgtgctgc ccagttggtt ccggcctgaa gcgggcccgt ctccgaacgc
     2281 gatctggctc accggtaccg gatctcctcc aagtcagcgc gctcgaggaa acgcgcgagc
     2341 agctggatgc ttgcgtcgat atctcgctcg tgcgcggctt cgttggcaga gtgcatgtag
     2401 cggcagggaa tcgagatcgt gccggtgtag gcgccaccgc gggtgcgctg gatggcgccg
     2461 gcatccgtcc ctccgcgagg caggacctcc aactggtgtg gaatccgctc gctctccgcg
     2521 atggaacgaa gcgtctctat gattgtgggg tgggggagct gcgaggaatc aaacactttt
     2581 attgcggtgc cggcgccgag gcgcgtgacg gcctcctccg gcggtatgcc ggggatatcg
     2641 ccggcaatcg tgacgtccag cgcgatcgcg atgtccggct cgaggtgaaa cgcggcggtc
     2701 tgggcgccgc gcaggccgac ctcctcctgg gtcgtggcga cggagatgat ctcagcattg
     2761 gtttcatcga cgtcccgaag cgcctcgatc atcacataaa ggccgacgcg gtcgtccagc
     2821 gccttgctga ccaccaaatc gcccatccgt acgctgggcc ggtcgagggt caccatgtcc
     2881 ccgatcgaaa ccagcgccct gacggcgtcc acagacaggc ctgtgtcgat gaagagatcc
     2941 tcaagcttta actccctgga ctctcccgcc ttcgtcaggt gcacaggctt ggtaccgggc
     3001 tgcagcgccc cggccagcgc ctggccatcg cgggtgtgga ctcgcaccct ttgggcggcc
     3061 agaacacgag ggtcgaagcc accgacctgc tgaacgcgaa taaacgcgtt gtcgtcgatg
     3121 tgacggacaa cgaagccgat ctcgtccatg tgggcggcga ccatgatgcg tggaccagat
     3181 cccctgcgga tggagacgag gttgcccaac gcgtcgacgt actgctcatc gacgtgtgcc
     3241 ttcagttctt cgcgaacgag atcgcggacg cgacgctcgc gagagccaat gccggctact
     3301 tggcagagtc gttcaaggag atcgaagttc atttggagct ctctcggtaa tgctgcatcc
     3361 cgcgtcggac ctgacggcag gtacgaaaca gggtgaccgc atgtcgcagc caccctgagc
     3421 gcgctacgtc tgccgcgagg acggtcgctg gctactcgcc gtctataagg atgaacgttg
     3481 acgacgcctc aacatctcct tcctcgtaca taactgtggc gagaccatgg atgttggcga
     3541 accgatcttc ctgaaccact ggactcgcgg acggcgtggc gccagcgaac gggtagtcct
     3601 cgcaagcctc gccatcgaag tcgaggtcga gcaggaagtt cgtgaacgga tcctcgtcgt
     3661 acgccgcctg ggcctccgcc tgcgaggcga actcagcgca ggcaggatcg gcgtcttcga
     3721 gcgcgaagtc ctgtccctcg gatagctccg tggccatcgt caccacctga tgaaggtcga
     3781 tgtaggtgag gtggttgtac tcatccggca ggcgcgccat gagcgtctcg aaggtctcgt
     3841 cctgtgagag cggctgggca ggacccgtca agtagtcgtc gagaccgctg ccgaccgaga
     3901 tcaccacctc gtcgtcgatc acaccgaact cgatcttgct gtcgatgccg gctgtgtcct
     3961 cggagagggc aatctgataa acctgcgagc cgtccacgtc ccgcgtttcg atgctgccct
     4021 ggtcgccgag cgcggctccc gcgatgaagg agatcttcga catgacgtcc tggacgacca
     4081 ccggattggt gatgtcgctc acgatcagtg cgtcgatggc cggcgtctcc gccgtaatgt
     4141 cgtcgacgga gacgaagatg ccgtaccgac cggtcagatt gtcgatgaag tcggtcttga
     4201 tgttgaagcc cagcaaggat tccgaccgct caaagacgtc gttctgcgcc tgcgccacgt
     4261 cgacctgatc gaccggcgtc gccatcagat cctcaccgac gagctctgtg gcgaagagaa
     4321 aggcgagcaa ggtcacgaga ccagaagagt cgatgtcggt accggtcgta acgaaaacgg
     4381 cgtcggccgg cactgtgtcg aggaaagcac cgtcgagcgc aacgggttcg gattccacca
     4441 actcattcgg cagggagcgc gtctccagcc ggaacccctg cggctcggcg gagaaggtga
     4501 acgcggtcca ggcgtcgagc gcggcgaggg tgttgacgtc cacggaggcg atcgcctcag
     4561 gcgacgaggc ctcgaccgcc tcgagcaatg ccacgccgtt gatccagccg ctcgcaacgt
     4621 gctcgctcgg cagcaggtcc gtcagcaact ggtaccgctc gctacttgcc agggaggtca
     4681 gatcctcgtt gaacgtgtcg ataagcgggt ggaggtcctc ggcgcgcatg gcgaacactc
     4741 gataatcgcc gatggttgcc acggcagtgc cttcagcaaa cgcatcgctg ggctcgaacg
     4801 ccgtgatggt gacaccgccg tgctcggcct cggtaatctc gcccaaaccg gcgccggtgt
     4861 cggcttccat gcgcgctcgc tcatccgcga tgaactgctc ggagccttcg ctggacacga
     4921 tgaccgcgaa tccccccccg tcgaatgact tggtgactgc catcgggtcg gtcgtgacat
     4981 cgcccacctc gtcgagtgcc tcaaggtccg gaatgcttgt caggacaaag gccacgtgaa
     5041 ggccctccgg gatctcgtcg gtacctgcct cctcctcgag caagtcggca aggccggcgc
     5101 gctgcaggag gtccgccgcg agcgccccct gctcggtgga cgtgtcgagg ttcgcctcga
     5161 catacacgta agcagtctcc ggcacgatat cgacaggttc gtttgtcaca aacgtctgcg
     5221 cgctcgctgg agcgagcggc acgatgagcg cgctcaccag ggcgaatgcg accagtagca
     5281 tcatccaatg cgctacagag aagccaacag tcagcgtatg ggttcgactc tgcacagaga
     5341 tctcctcgcc ggtgataggc ggccacaatc gccacgtctt gccgttttcc aaacagggac
     5401 ccgacagcca atggtaccgg gcgcggacga tgcggtcaac agaagctgtc aggtattccg
     5461 gtcctcccct ggctaaatgt ttcggtcgat gccggtcgcc atggcgagaa tgatgatgaa
     5521 cagtgctgcc gtgagcaagg cgacgacgac gaacacgccc agcgtggcga ccaccttgag
     5581 cagttctgcc agaatttcca ccgtgagtcc tccgttatgg ccgccgtcgc gcggcccgtg
     5641 gacaggtaca gccgtttcat ctcgcagtat atccggtccc gagcagaccc atgcgccaaa
     5701 gaatgaaccc ccactcccgg ttcgcacccg ggagtggagg ctggctgatg gattgatgaa
     5761 tgtcaggcga cgaccggcgc ctcggtgagc tggccgcgca ggatgaagtc gttgtcctcg
     5821 acgccgaggg taatagtttc cccctcgtga agcgcgccgc tcagcagggc gagcgctagc
     5881 gggtcgagca cgcgcttctg gatcacgcgc ttgaggggcc gagcaccgaa cgccgggtca
     5941 tatccgagcg tcgcgagcag gtcccgggcc tcctcggtta cctgcagatc catgttccgg
     6001 tcaccaaggc gactgcggat ctgatcgagc tgcagctcga cgatttcggc gatctcctcc
     6061 cgagagagct gcgtgaagat gatcgtctcg tcgatgcggt tgaggaactc gggtcggaag
     6121 ttggcgcgga gcgcgtccat gaccttggcg cgaatctcgc ccgcgttgct ctcgttcatc
     6181 tgcatgatcg cctgcgagcc caggttgctc gtcatgatga ccaccgtgtt ggtgaaattc
     6241 accacccggc cctggccgtc cgtcaggcga ccatcatcca gcacctgcag caggatgttg
     6301 aatacatccg gatgcgcctt ttcgatctca tcgaaaagga tcacgctgta gggccggcgg
     6361 cgaatcgctt ccgtcagctg tccaccctgg tcgtaaccga cgtaccccgg aggcgctcca
     6421 atgagccggc tgacggcgtg cttctccatg tactcggaca tgtcgatccg caccattgcc
     6481 tggtcgctgt cgaagaggaa ctccgccaga gcacgcgcca gctccgtctt gccgacgccg
     6541 gtcggtccca ggaagatgaa gctgcccatc ggccgattcg gatcggagag cccggcccgt
     6601 gaacgccgga tggcgttgga cacggcctcg atcgcctcgt cctgcccgat caccctggcg
     6661 tgaaggcggt cttccatgtg gaccagtttc tcgatttcgc cctccatgag gcggctgatg
     6721 ggaacgccgg tccagcggct gacgacctcg gcgatgtcct cggcgtcgac ctcctccttg
     6781 agcagcttgc cactcgactg aagtcgagcg agccgagcct cttcctcctg gagcttctgc
     6841 tcgagctcct tggcccgacc gtactgcagc tcggcagcgc ggttgtaatc gtaatttcgc
     6901 tgcgcctgct caatttccac ccggaggtgg tccatctcat cacgcagctc ggagattgcg
     6961 atgatggctt ggcgttcctg gtcccagcgg gagcgaagcg catcgcgctc ctccttgagg
     7021 ttcgcgagct cctcctcgag tgcctcgagc cgctgcttcg acgcctgatc agtctccttg
     7081 cggagtgcct cgcgctcgat ctcaagctgc atgatgcgcc gctcggcctc atcaagctcc
     7141 actggcatcg aggtgatctc catccggaga cgggacgcgg cctcgtcgac gagatcaatc
     7201 gccttgtcgg gcaggaaacg ctcggtgatg taccggtcgg agagcgttgc cgccgcaacc
     7261 aacgccgagt caaggatctt caccttgtgg tgcacctcat accgctcgcg gagaccgcga
     7321 aggatggaga tcgtgtcctc gacagtgggc tcgccgacca gcaccggctg gaagcgccgc
     7381 tccagcgcgg cgtccttctc gatgtgcttg cggtactcgt ccagagtcgt cgcgccgatc
     7441 gcgtgcaact cgccgcgcgc cagcatcggc ttgagcatgt tggaagcatc catcgagccc
     7501 tcggcggccc cggccccgac gacggtgtgc agctcgtcga tgaagaggat gacctggccc
     7561 tcgctcgact gaacctcttt gaggacggcc ttcaggcgct cctcgaactc gccgcggaac
     7621 ttcgcgcctg ccacaagtgc accgaggtcg agagaaatga ttcgcttgtc tttcagtccc
     7681 tcgggcacat cgccgcgcac gatacgctgc gccaggccct cggcaatcgc ggtcttgccg
     7741 acgcccggct cgccgatgag caccgggttg ttcttggttc ggcgcgagag cacctgaatg
     7801 acgcgccgaa tctcgtcatc acgaccgaca acgggatcga gcttgccgtc acgggcaagc
     7861 tcggtcagat cgcgcccgta cttctcgagt gcctggtagg tattctccgg gttctcatcg
     7921 gtcacgcgct ggttgccgcg gatctcggtc agcgcgccca gcactcgatc ccggtctgct
     7981 cctagccggt tcagaaggga ggccgcctgc gtgcggtcgc ctccaagaat gcccagcagc
     8041 aggtgctctg tgctgatgaa ctcgtcaccg aactgctgcg cctggcgctc ggcgtcctga
     8101 agcgccttgc ggagcgcctg gctgaccacg gcctctgcgc tgtactgcag cttcggagcg
     8161 cggtcgatca atgcgttcgc ctcttggagc accacagcgg ggtcaaggtt cagtcgctgc
     8221 aggatttgcg gcacaattcc gtcgggctgg tcgacaagcc cgaccagcag cgcctccggc
     8281 tcgtactggg cgagattctt ctcctgcgtg atttgctgcc cggcgataat cgcctgccgg
     8341 gccttttccg tgaagttctg gtccgttgcc atctggttgt cttctccttc agggtcagcg
     8401 ttgactcgtg gagcgaaatc gttcccgcgg ctaccggcgc cgcgcgttct ctcgaacctg
     8461 ctgctgctca acaaaaccaa gctcgaccag aatccgatcg aggtcgtccg tccacgcccg
     8521 gctgtccggt gatcgaccca tgctcgcgcg gagcttgcga agcgattgcg tcaactcgag
     8581 agcgagctgc acgccggcca ggttgaggcc gcgtgtctcc accaggtact tgacctgccg
     8641 gagccgttca atgtcctcgt cagagtagag ccgcagattg cccgcggttc gcgacggggc
     8701 gatcagcccc tcacgttcgt acttgcgcag cgtctgcgga tgcatctcca cgagccgcgc
     8761 cgccacgctg atgacgaaaa ggggttctga atatcgagat gccatgcgcg tatgactcct
     8821 gtcgtccaac catatgcagg aataatactt cttgatacga ccgatgtcaa tgttggtgcg
     8881 gcctgctaca ggagaagcgg cccatccggc cgtaagttga gccacaacgc ccgcgtaggg
     8941 gataatcctg gcacgtgaga ggtgccagga actggcaggt cggggcgtgg cgagcccggc
     9001 tgcagccgat ggcaacgaag gggtagacgc gctttggcca acctcataac cgtggcacgc
     9061 ctgatcctgc tcttcgttgt gatttggctg atttacgtcg gtaacgtcca agtggtaact
     9121 gcaagcatga tgctcatcat catcgtcttc gccagcgatg gcctggacgg atgggtggcg
     9181 cggaagcgcg gatcgacatc gaccttcggc gccgtgttcg acatactcgg cgaccgcatc
     9241 gtcgagaacg cgctgtgggt cattttctcg gacctcgatc tcatcccggt ctggatacca
     9301 ctcatggtgc tcacccgagg gttcatcgtc gatggagtgc gctcgatgtc ctacgcggaa
     9361 gggaagacgc cattcggcaa gaacaacatg atgaagtccg cgttcaccga gtggctgacc
     9421 gctggccggt tcatgcggag tttcttcggc tgggcgaagg ctgcggggtt cgtctttctc
     9481 accggtcttg ccggaaagga gcgcctcgac accggcggga ccttcctcgg cacgctttac
     9541 gaccagcgct ggtatttgtg gcttggctgg ggcctggtct gggcggcggt gatactcact
     9601 atcgtcagag gggtaccggt catcgtcgat gcctgggcgc acatccaggg cgatgccgag
     9661 cagccgctgt ctgcatgaaa tggccgtgat acgttcgtga cctggggaag ctgtgccgaa
     9721 tgacagaggg acggtccgac aggccgtccg cgagttccga caggaagagc ggcaggagtt
     9781 ccggtatctg ctgagcgtga cgctcgccat cgggttctct tgggttttct ggctgatccc
     9841 cggtcccatt cgtggctgga ttgccgacca ctccggtgat cttttctatc ggatcagcaa
     9901 cacctaccca gacaacgttc gggacaacgt ccagaccgtg cttgattaca ttggcgacgc
     9961 gcggatggac ccggagccgc tggttccctc gatcttccgg accagcgccc gcaacttcat
    10021 ggacctcatc atcacacctc gccagcccgc tcgcagcttc ctgacctcgg tacggctcgt
    10081 gagcggatca tggcagacgc tcgatcgcgt actggaggcc gggaagggcg gtattctcgt
    10141 cagcggccac gtcggtagca tcgactacat cggccaggtg ctcacggtcc ggggctacaa
    10201 gctgacggtg gtgaccgggc gtacgacgtc gcggttcatc ttcgacgggg tcagctggct
    10261 gcgcgcctcg cgcggcgcca cgctggtcga gccgactcca tccggcattc gtaaggtcat
    10321 gaaggccgtg cgtcgcaacg agttcgccgt cttcgtcacc gaccgggact tctttcagaa
    10381 cggccaggac gtggtcttca tgggccgcca gacgacgctc cccccgggac cggtgcgcat
    10441 cgcacgggag acgggcgcgc cgctcattcc cgtcttcacg cgccgtgtcg cgaggggaca
    10501 cgagctaacg atcagcgagc cgttccatat cgagcgcaca tcggacgtca gggccgacat
    10561 ggcggtcggg atggagcgac tcgtggaggt cttggaggcc gggatcattt cctcgctcga
    10621 tcaatgggtg atgttccagc gggtgtggct ggagccccag cgtgtgccgg tgcgcgtctt
    10681 tccggtggga tccccgctgg aaagcgagct gctggagcg
//
LOCUS       DASYWL010000148         5390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_344559, whole genome shotgun sequence.
ACCESSION   DASYWL010000148 DASYWL010000000
VERSION     DASYWL010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5390
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344559"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VGR22_05190"
     CDS             complement(<1..387)
                     /locus_tag="VGR22_05190"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEV2127997.1"
                     /translation="MATSEDQLLTTLREKLYVPVVSDVLDALGYLDRAMRADLRPLKP
                     HMRIIGRAHTVLTTDVYKRPEEPYKLEIESVDALEPGDVMVVCTNGSTRTCFWGELLS
                     TAAVARGATGCVIDGHTRDALRIMEMD"
     gene            529..1038
                     /locus_tag="VGR22_05195"
     CDS             529..1038
                     /locus_tag="VGR22_05195"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2127998.1"
                     /translation="MGSGSSDMNERHDDPTIRPARPDEAGELTGIAMRSKAWWGYDDH
                     FMETVRPELTVTPEQIARDHVVVIERGARLAGFAHLRRESDTTLELVSLFIDAWAIRQ
                     GLGQRLWNEAIAYARGEGYRAVTLEADPNAEPFYALQGAVVTGSRESTLMAGRVLNLM
                     TLTLDGDNP"
     gene            1201..1428
                     /locus_tag="VGR22_05200"
     CDS             1201..1428
                     /locus_tag="VGR22_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2127999.1"
                     /translation="MSSNATQQAGEAQQEYFRVTDSVPEQTWYWLALASIGISAALKI
                     ADKDHWALFVGQWPPTFLLFGIFHRVVRPGR"
     gene            complement(1528..2181)
                     /locus_tag="VGR22_05205"
     CDS             complement(1528..2181)
                     /locus_tag="VGR22_05205"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEV2128000.1"
                     /translation="MAATPTLDVVIPVGRAVLRGMLAVPEGAQGIVAFAHGSGSSRLS
                     PRNQEVARSLQAAGFGTLLFDLLTQDEAVVDERTREHRFDIPLLGERMVAAIDWLGEQ
                     GGAASLPIGLFGASTGAAAALVAAAERPDDVAAVVSRGGRPDLAGEALTRVAAPTLLI
                     VGGHDAPVIGMNESAQARLRGESRLVIVPGATHLFEEPGTMEQVTDHVIRWFGEHLR"
     gene            complement(2194..2397)
                     /locus_tag="VGR22_05210"
     CDS             complement(2194..2397)
                     /locus_tag="VGR22_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128001.1"
                     /translation="MWWQDLLWGFWNGITAWIVLIAHVFGWWDHFPLYDVARSGNWYD
                     FGFLLGTGSPFLGTFGGSRASRT"
     gene            complement(2426..4477)
                     /locus_tag="VGR22_05215"
     CDS             complement(2426..4477)
                     /locus_tag="VGR22_05215"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HEV2128002.1"
                     /translation="MALPFQDRREAGKALAQDLNDLRGRDDVIVLGLPRGGIPVAYEV
                     AMAVDAPLDAYIVRKLGVPWHEELAMGAIASDGVKILNEEIVKGEGVNEDAIEAVVAR
                     ETSELRRRERLYRGTQPPPDVKDRTVVLVDDGLATGTTMRAAVQAVQQLGPRSVIVAV
                     PVAAPSVVRSFEGMVNRIVASATPEHFYALGLWYQDFTQTTDQEVRDLLAKAAEREDV
                     ALPVRSAGANRSPGSPPLRDTIAQQATPLSGDAREYDRLLDDIGDARLVLIGEASHGT
                     EEFYRERAVITQRLIEERGFHAVAAEADWPDAFRVNRYVRDVSDDRSAVESLNDFQRF
                     PQWMWRNTVARDFIVWLRTHNDEQQNDRQRAGFYGLDLYSLSASIQAVIDYLDQTDPE
                     AAARARARYACFDQFADDAQSYGYAASLGVAESCEDEVVNQLLEMQRQAADVAQRDGQ
                     RAEDAFFSAEQNARLVRNAETYYRSMFRGRVSSWNLRDSHMADTLDELLEYLTERHGE
                     PAKIVVWAHNSHLGDARATQMGRAGEHNLGQLARERHGDDAYLIGQTTHHGTVAAASD
                     WGDPAERRRVRPGLPDSIEKLFHDVEHDDFLLLLRSADERLNDRRLERAIGVIYRPES
                     ERVSHYFEARLQDQFDAVLHFDETAALEPLEPTEEWARGEAPETFPFAGEPIPGGRRS
                     V"
     gene            complement(4638..4946)
                     /locus_tag="VGR22_05220"
     CDS             complement(4638..4946)
                     /locus_tag="VGR22_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128003.1"
                     /translation="MHPHQLRQPGVEFGGESTDPGINMIYLPSETVEKLDNGETDTGD
                     DGEAPDQGEDTSGTTALPETGTGATALGSRHQFDAMILLVAALDLLGAACVTRRLPAR
                     "
     gene            complement(5172..>5390)
                     /locus_tag="VGR22_05225"
     CDS             complement(5172..>5390)
                     /locus_tag="VGR22_05225"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2128004.1"
                     /translation="DMSNEQPDEWMTVQEVASDLKVNEETVRRWIRNGELDVLALGTS
                     NRAGYRIRKRALDTFIAERYGPKGKAAA"
BASE COUNT          867 a         1864 c         1728 g          931 t
ORIGIN      
        1 gtccatctcc atgatgcgca gcgcgtcgcg ggtgtggccg tcgatcacgc agccggtcgc
       61 cccgcgggcc accgccgccg ttgagagtag ttcgccccag aagcaggtcc gtgtcgagcc
      121 attggtgcac accaccatta catcccccgg ctccagcgcg tcgaccgact cgatctcgag
      181 tttgtagggc tcctccgggc gcttatagac gtcggtggtc agcaccgtat gcgcccgccc
      241 gatgatccgc atgtgtggct tgagcggacg gagatcggcg cgcatcgccc ggtcgagata
      301 gcccagcgca tccagcacat cggagacaac cggcacgtag agcttctccc gcagcgtggt
      361 cagcagctga tcttcgctgg ttgccatcgt tcatcgcctc cggtgtggac gggacaggaa
      421 tcgatgcacc acggtagcag cgacggggct atcgggcaat cgatcgtcat ggaatggcag
      481 tatggtctct taaagtacag ggcaagctcg cgcagcggag cagggacgat gggatcaggc
      541 agcagcgaca tgaacgaacg ccatgacgat ccaacgatcc gaccagcgcg tcctgacgag
      601 gccggcgagc tgaccgggat cgcgatgcgg tcgaaagcct ggtggggcta cgacgaccac
      661 ttcatggaaa ctgtccgccc cgaactgacg gtcacgcccg agcagatcgc gcgcgaccat
      721 gtcgtcgtaa tcgagcgagg cgctcgcctt gccgggttcg cccacctccg tcgcgagagc
      781 gatacaacac tcgagctagt ctcgctcttc atcgacgcct gggcgattcg tcagggtctc
      841 gggcagcgac tgtggaatga ggcaatcgcc tatgcgcgag gagaggggta ccgagcggtc
      901 acgctggagg ctgatcccaa cgccgagccg ttctatgcct tgcagggtgc tgtcgtcact
      961 ggcagtcgcg agtcgaccct gatggccggc cgcgtgctca atctcatgac gctgactctg
     1021 gacggcgaca atccataagg gccaacccac ccacctatcg acatcgatcg tgccggacgg
     1081 cgggccaagg gagtgatgca cttgcacatt tcggcgatat cagcggcaca cctcatgcat
     1141 tacaccacag caggtcgatg ggaccaaggt cgtcaattta tcggtaaagg agcaacgtgc
     1201 atgagtagca acgcaaccca gcaggccggt gaggcgcaac aggagtactt tcgggtcacg
     1261 gacagcgtgc ccgagcagac gtggtactgg ctagcgctgg cctcgatcgg gatctccgcg
     1321 gcactgaaga tcgccgacaa ggatcattgg gccctgttcg tcgggcagtg gccaccgacc
     1381 ttcctgctgt tcggcatctt ccaccgggtg gttcgccccg gcaggtagat tgtcatcgac
     1441 accagggcgc ctcgcgaaac tcttcgcgag gcgccctttt acgtgatgga ggttttgcag
     1501 cagtgatggc gagcatgccg gtagtcctca ccgcaggtgt tccccaaacc agcggattac
     1561 gtggtcggtg acctgctcca tcgtgccggg ctcctcgaac aggtgcgtgg cgccgggcac
     1621 gatcaccagg cgcgactcgc cccgcagccg cgcctgcgcc gactcgttca tgccgatgac
     1681 cggggcgtca tgcccgccga cgatcagcag ggtcggcgcc gcaacccggg tgagcgcctc
     1741 gcccgcgaga tcggggcgcc ctccgcgcga gaccactgcc gcaacgtcgt ccggtcgttc
     1801 cgccgcggcg accagcgccg ccgcggcccc cgtgctggca ccaaacaggc cgatcggcag
     1861 cgacgccgct cctccctgct cgccgagcca gtcaatcgcg gccaccatcc gctcgccgag
     1921 cagcggaatg tcgaagcgat gctcgcgcgt acgctcgtcg acgaccgctt cgtcttgggt
     1981 gaggagatcg aagagcagcg tcccgaaccc cgccgcctgc agcgagcgcg cgacctcctg
     2041 attgcgcggg ctcaaccggc tgctgccgct gccgtgcgcg aaggcgacga tcccctgtgc
     2101 cccctccggc accgcgagca tgccgcgcag cacggcgcgc ccgaccggaa tcacgacatc
     2161 gagcgttggt gttgccgcca ttgtggtgcc ctcctacgtg cgagacgccc tgctgccacc
     2221 gaatgtcccc aggaacggcg atccggtgcc cagcaggaat ccgaagtcgt accagttgcc
     2281 gctccgagcg acatcgtaga gcggaaagtg gtcccaccag ccgaacacgt gcgcgatcag
     2341 gacgatccat gccgtgatgc cgttccagaa gccccagagc agatcctgcc accacatcgc
     2401 tggtcactcc ccacgtgttc ggaggtcaca ccgatcggcg gccgcccggg atcggctcgc
     2461 ccgcgaacgg gaacgtctcc ggggcctcgc cgcgcgccca ctcctccgtc ggctcgagcg
     2521 gctccagcgc ggccgtctcg tcgaaatgca gcaccgcatc gaactggtcc tgcagccgcg
     2581 cctcgaagta gtggctgacg cgctcgctct ccggccggta gatcacgccg atcgcccgct
     2641 ccaagcgccg gtcgttcaga cgctcgtcgg cgctccgcag caagagcagg aagtcgtcgt
     2701 gctcgacgtc gtggaagagc ttctcgatcg agtcgggcag gcccgggcgt acccgcctgc
     2761 gctcggccgg gtcgccccag tcgctggccg ccgcgaccgt gccgtggtgt gtcgtctgtc
     2821 cgatcaggta ggcgtcgtcc ccgtgccgct cgcgggccag ctggccgaga ttgtgctcgc
     2881 cggcgcggcc catctgcgtc gcgcgggcgt cacccagatg ggagttgtgc gcccagacca
     2941 cgatctttgc cggctcgccg tggcgctcgg tcaggtactc cagcagctcg tcgagcgtat
     3001 ccgccatgtg ggagtcacgc aggttccacg acgacacccg gccccggaac atcgaccggt
     3061 agtaggtctc ggcgttgcga accagccggg cgttctgctc ggcggagaaa aaggcgtcct
     3121 cggcgcgctg cccgtcacgt tgggcgacat cggccgcctg ccgttgcatc tccagcagct
     3181 ggttgacgac ctcgtcctcg cacgactccg ctacgcccag gcttgccgcg tagccgtacg
     3241 actgcgcgtc gtcggcaaac tggtcgaagc aggcgtaccg tgccctcgcg cgggcggcgg
     3301 cctccgggtc ggtctggtcc aagtagtcga tcactgcctg aatggacgcg ctgaggctgt
     3361 agagatcgag cccatagaac ccggctcgtt ggcggtcgtt ctgctgctcg tcattgtgcg
     3421 ttcgcagcca gacaatgaag tcgcgggcca ccgtgttgcg ccacatccac tgtgggaagc
     3481 gctggaaatc gttcagcgac tcgaccgccg agcggtcgtc gctgacatcg cggacgtagc
     3541 ggttgacgcg gaaggcgtcc ggccagtcag cctcggctgc cactgcatgg aacccgcgct
     3601 cctcgatcag ccgctgcgtg atgaccgcgc gctcccggta gaactcctcg gtgccgtgtg
     3661 acgcctcgcc gatcagcacc aggcgcgcgt cgccgatgtc gtcgagcagc cggtcgtact
     3721 cgcgcgcgtc tccgctgagt ggggtcgcct gctgcgcgat ggtgtcccgt agcggcggcg
     3781 accccggcga tcggttcgcg ccggcagagc ggaccggcaa tgccacatcc tcccgctcgg
     3841 ccgccttcgc cagcaggtcg cgcacctctt ggtcggtggt ctgggtgaag tcctggtacc
     3901 agagcccaag cgcgtagaag tgctccggcg tggcgctggc gacgatccgg ttgaccattc
     3961 cctcgaagga gcgcaccact gacggcgccg ccaccgggac ggccacgatg acactgcgtg
     4021 gtccaagctg ctggacagcc tgcaccgccg cccgcatggt cgtgccggtg gcgagtccgt
     4081 catcgaccag caccaccgtg cggtccttca cgtcgggcgg gggctgcgtg ccccggtaga
     4141 gccgctcgcg gcgtcgcagc tcgctggtct ctcgggccac caccgcctcg atcgcgtcct
     4201 cgttgacgcc ttcccccttg acaatctcct cgttgaggat cttcacgccg tcgctggcga
     4261 tcgcacccat cgccaactcc tcgtgccatg gcacgccgag cttgcgcacg atgtaggcgt
     4321 ccagcggtgc gtcgaccgcc atggcgacct cgtaggcgac cgggatgccg ccgcggggca
     4381 gcccgagtac gatcacgtcg tcccgcccgc gcaggtcgtt caggtcctgc gccagcgcct
     4441 tcccggcttc acgtcggtcc tggaatggaa gtgccatgtc tgcctccgtt tgtcgcattt
     4501 cgcgtcgata caggtagcga caaggcaata ttcagcacat cccacgccac cgacccaacg
     4561 cgaaacgggt atggacttcg aagtccacgc ccgttgacgt ggcgttcagt ccatgacacc
     4621 ggcataccga caaagggtca gcgcgcgggc aatcggcgtg tcacgcaagc ggcacccaac
     4681 aggtccagtg ccgcaaccag caggatcatc gcgtcgaact ggtgacgtga accgagcgct
     4741 gtcgcgccgg tgcctgtctc cggcagcgcc gtggttcccg acgtatcctc gccctggtcc
     4801 ggggcttcgc cgtcgtcccc ggtgtcggtc tcgccgttgt ccagcttctc gacggtctcg
     4861 gacggcaggt agatcatgtt gatccccgga tcggtagatt ctccgccgaa ttcgaccccc
     4921 ggctgacgga gctggtgcgg gtgcaccgat cggcctccgt tggtcggtgg cgaaaatctc
     4981 tcgtgatgcc tagggatgcc tagcccacgg gcgcggctgt cttccgggta acggccagat
     5041 cgcgcaaccg gctcccctgg gatcgggagc ctaggtcgcc aggaccgcga gcggtatact
     5101 ctgaccgcgc ctcatccgac gctgactccg ccggatcgag gggttccaag aagccgcagc
     5161 ctagggctca tctaggctgc ggcttttccc ttcgggccgt agcgctccgc gatgaacgtg
     5221 tccaatgccc gcttgcggat gcgatagccc gctcggttgc tcgtgcccag cgcgagaacg
     5281 tccagctcgc cgttccggat ccagcggcgg acggtctcct cgttgacctt gagatcgctc
     5341 gcgacctcct gcacggtcat ccattcgtct ggctgctcgt tgctcatgtc
//
LOCUS       DASYWL010000149         6965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_645010, whole genome shotgun sequence.
ACCESSION   DASYWL010000149 DASYWL010000000
VERSION     DASYWL010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6965
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645010"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VGR22_05230"
     CDS             <1..790
                     /locus_tag="VGR22_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV2128005.1"
                     /translation="TTIGREKRENYAFQPETADEFKEAVMGGMPSAPTYYPELKKVNK
                     AGAGPITQLPAGEELDPGTVAARQAEGAMIVDAREKGEVAKGYIPGSVFAGADNNMTT
                     WMGWLAPYEGDIVLIVDGREEYEQARLMLQRIGLDRVAGYLIGVEGWKQSGRELDTIG
                     EVTVEQVSEVVGTPGAPIILDVRSDSEYEEGHIPGAKHHYAGEITQGDMPNLPKDADI
                     VVVCGSGYRSMVASTLMKDAGFQKLASMVGGMEAWNEAKEEVTA"
     gene            complement(972..1949)
                     /locus_tag="VGR22_05235"
     CDS             complement(972..1949)
                     /locus_tag="VGR22_05235"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV2128006.1"
                     /translation="MGAGPAGLAAAVYAASEGLRTAVFEPVALGGQAGMSSMIRNYLG
                     FPLGISGEEITKRAHEQALHFGATVVHTHAAVALRAEGDLRVVTMSNGAEVRARTVVL
                     ATGVAYRRLEAPALHRLIGAGVFYGAATTEARALSGEDVYVVGAGNSAGQAALHLAEF
                     ARQVTLVVRGESLARSMSTYLIKQICATPNIAVRLRTQVVDGNGTSRLEQLVLQDADG
                     VRFAVSARALFVLVGARPRTEWLDGSVHRDAGGYIVTCHDLDQQISGITASWPLDRLS
                     LPMETSMPGVYAVGDVRHQSVKRVASAVGEGAIAVFSIHRYLAEQSARR"
     gene            complement(1844..2545)
                     /locus_tag="VGR22_05240"
     CDS             complement(1844..2545)
                     /locus_tag="VGR22_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128007.1"
                     /translation="MATSVPLADAILQRYRDEGIEVALVLADFRLAGANGITFLAGIR
                     PCHPRARRALLTTLGDHEAATPIHQAMALGQLDLIVSWPWESPEESLYPRVSEALATW
                     WHTNRPAFERVRVIGKQWDPQSHFLRDMGKRNGVPFGFYGADSTVGCHMLREMEAEGL
                     ELPVVSIDGKYSPIRRSLTSRTCSAPRRRCPRKSATSPSWAPAPPAWRQPSTPHRKGC
                     GPPSSNRSRSAGRRA"
     gene            complement(3027..4067)
                     /locus_tag="VGR22_05245"
     CDS             complement(3027..4067)
                     /locus_tag="VGR22_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128008.1"
                     /translation="MHVEEARSIARRWLAEQAAPLPGFRGALWHGSILTIPDDAPLDP
                     ASDVDLMVVFDTPPADLRPGKQPVEGVLLDLTVLPWADIRDPESVLANHQLAPSFRSP
                     ELLADPDGTLGALRDTIAREFARRPWVERRVEAAAGKIGDSLGRIGPSAPFAENTMSW
                     LFGTGITTHVLLVAGLRNPTVRKRYIAARELLVSTGMPSVHEELLGLLGSKELSPERI
                     RHHLDTVEPAFDAAVSAIRTPVLFPASDLSPAARPIAIDGSRSMIAAGDHREAVFWIA
                     VTWARCMQVLHADSPELETRFGPAFRGMLADLGIQSVTDMQSRAAEIQAALPHLRATA
                     HEIMDVIPKIED"
     gene            4318..4605
                     /locus_tag="VGR22_05250"
     CDS             4318..4605
                     /locus_tag="VGR22_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128009.1"
                     /translation="MMYRHFSNRERSAFRALSIAVTSFLLALTLLVPPAAAGRTGPLV
                     DDRRSGESGAPAVVERSWDQYQIDLLEDRKPSSSFDGGNSSNYCYVCYKYR"
     gene            4766..5473
                     /locus_tag="VGR22_05255"
     CDS             4766..5473
                     /locus_tag="VGR22_05255"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2128010.1"
                     /translation="MMAGGARCRLDTKAIGCRIDQPLSGSPEPLHLHLYPGKQAGGWH
                     VAAPLTSILGRDAEIDAVVAQLDRGEVRLVTLTGPGGIGKTRLVREVALRMQEHFAHG
                     ACFVALAAVPDPTLVPSAMASALGLQLLTDRPAEELVSEAPRDRHVLLVLDNLEHLAE
                     QSAPWVVALLEQCPRVSALATSRVPLRIAGEHRFPVDPLALPEDRSGPEARLETIAES
                     AADDWQRGGGRADLPHA"
     gene            5760..>6965
                     /locus_tag="VGR22_05260"
     CDS             5760..>6965
                     /locus_tag="VGR22_05260"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2128011.1"
                     /translation="MLELVEHSFVRRREQGPDGYRFSMLEVVREFGAGQLEAAGELTA
                     AREAHAAWVLELARKAEPRMAGPEQVEWLDRLAQEHDNIRRVVGWLTERERIEEALDL
                     TGSLWFFRWIRGFYDEARRQFDILLALPAGGDRTVARAKALIGLGIVASHQGDVPHAL
                     EAFEEAIAILQVLDEQWHLSLALACYGTAYHMIGRIDECERHLSESQATADRLGDAWL
                     WQGSICNFGVIALSRGQWDRGRQFMEESVRASRSIGDRWGLSIGELNLGYIDMHEGNL
                     DSAESHIQTSVRLIEELNDKRDLPGAYCSMAEILRQRGQLAEAKAMLDSALMVARDIG
                     DRTQIANALTGLAHIVHLQGEHRAAVDLMGDAMRSFERGGHLAAALMRLDDIADMALA
                     LGDGRHAAWC"
BASE COUNT         1194 a         2170 c         2428 g         1173 t
ORIGIN      
        1 gaccacgatc ggtcgcgaga agcgcgagaa ctacgcgttc cagccggaga ccgccgacga
       61 gttcaaggag gcggtgatgg gcggcatgcc gagcgcgccg acctactatc cggagctcaa
      121 gaaggtcaac aaggctggcg ccgggccgat cacgcagctc ccggcgggcg aggagctcga
      181 tcccgggacc gtggcagccc gacaggccga gggcgcgatg atcgtcgatg cccgcgagaa
      241 gggcgaggtc gcgaaggggt atatccccgg atcggtcttc gctggcgccg ataacaacat
      301 gaccacctgg atgggctggc tggccccgta tgaaggcgac atcgtcctga ttgttgacgg
      361 ccgcgaggag tacgagcagg cacggctgat gctgcagcgc atcggactgg accgcgtagc
      421 cggatacctg atcggcgtcg agggttggaa gcagtcaggc cgcgagctcg acacgatcgg
      481 cgaggtgact gtcgaacagg tcagcgaggt cgtgggaacg ccgggcgcac cgatcattct
      541 cgatgtgcgc agcgacagcg agtacgagga gggacacatc ccgggcgcga agcaccacta
      601 cgccggtgag atcacgcagg gcgacatgcc gaacctgccg aaggatgcgg acatcgtggt
      661 cgtctgtggc tcgggctatc ggtcgatggt cgcctcgacc ctgatgaagg atgctggatt
      721 ccagaagctg gccagcatgg tcggcggcat ggaggcctgg aacgaggcga aggaggaagt
      781 cacggcatag acgtttggtg cgtgcgcaga ggggagtcga gcgagcggta gcatccgttt
      841 tcccctgagt acaacgacgc cacgccgggc aggtgaagtc tcgacctgcc cggcgcccat
      901 gtgatgacga tacggttgtt tcgcgatcca ccggtcgcgg ccggtccgta ccgccgaatg
      961 aatcgagccg gtcatcggcg agctgactgc tccgcgaggt accggtggat cgagaagacg
     1021 gcgattgcgc cctcgccgac ggcagaggca acccgcttga ccgactggtg gcggacatcc
     1081 ccgacggcgt agacgcccgg catgctcgtc tccatcggga gcgagagccg atccagcggc
     1141 catgaggcgg tgatgccgct gatctgctga tcgaggtcgt ggcaggtgac gatgtagccg
     1201 cctgcgtcgc gatgcacgct gccgtccagc cactccgtgc gtgggcgagc gccgacgagc
     1261 acgaacagtg cccgcgctga caccgcaaat ctcaccccgt cggcgtcctg cagcaccagc
     1321 tgttcgagcc gggacgtgcc attgccgtcc acgacctggg tgcgcagccg cacggcaata
     1381 ttcggcgtcg cgcagatctg cttgatcaaa taggtggaca tgctgcgggc gagggactct
     1441 ccacggacca cgagcgtcac ctggcgcgcg aactccgcga ggtggagtgc agcttgtccg
     1501 gccgaattgc ccgcgccgac gacgtagacg tcttcgcccg acagcgctcg cgcctcggtc
     1561 gtggctgcgc catagaacac gccggcaccg atcagtcgat gcagcgcggg ggcttccaat
     1621 cgccggtagg cgacccctgt tgccagcacc actgtccggg cacgcacctc agccccattc
     1681 gacatggtca ccacgcgcag gtcgccttcg gcgcgaagcg ccacggcagc atgggtgtgc
     1741 accactgtcg cgccgaagtg cagcgcctgt tcgtgggccc gtttggtgat ctcctcgccg
     1801 ctgatgccga gcggaaagcc gaggtagttg cggatcatcg agctcatgcc cgcctgcccg
     1861 ccgagcgcga ccggttcgaa gacggcggtc cgcagccctt ccgatgcggc gtagacggct
     1921 gccgccaagc cggcggggcc ggcgcccatg atggcgacgt cgcagacttc ctcgggcacc
     1981 ttcgtcgtgg cgccgagcac gtccgcgatg tcagtgagcg acggattggc gagtatttgc
     2041 cgtcgatgga cactaccgga agctcgagtc cttccgcttc catctcgcgc agcatgtggc
     2101 agcccaccgt ggagtcagcg ccgtagaagc cgaagggtac gccgttgcgc ttgcccatgt
     2161 cgcggaggaa gtggctctga ggatcccact gcttgccgat cacgcggacc cgctcgaacg
     2221 ctggccggtt ggtatgccac catgtggcaa gggcttcgct cacccgcgga tagagcgatt
     2281 cctccggcga ctcccatggc caggagacga tcaggtcgag ctgtccgagg gccatcgcct
     2341 ggtgaatcgg cgtggcggct tcatggtcgc cgagtgtcgt gaggagcgcg cgcctcgctc
     2401 gggggtgaca cgggcggata ccggcgagaa acgtgatacc gttggcgcca gccagccgga
     2461 agtcggccag caccagcgca acctcgatcc cttcgtctcg gtaacgctgc agtatggcgt
     2521 cagccagcgg gacggatgtg gccacacgaa ctcggtagtc gggagcgtag cgccggttga
     2581 gattgtcccg cagcgcacca gcccttgggc tatcggtgat gacggcaagc agaaccggtg
     2641 ccgtagtcgg tcttgtagca gtccgtacgt cgcgtgatcg catgcgggtg agacggcggt
     2701 gcgggacggt ccggcggtcg gtgcgacggg cactcggtga tcgcaggaca ggtgcatcgg
     2761 ccaaaacatc cttgctgaca acggccattg ggcctcctgg tgatgagacg acggtgtcga
     2821 aaagatcccg ggccggggcg gtcgcgacgt ggcagtgcgc ggcgttgccc ggcattgtga
     2881 tgaagccgtc catggctccc atctgatcca accgtgccca cggtgcgtca acacaccgtc
     2941 aacgttctgc gcaggcgaca cacgaggagg cgccgtcaag aagatcggga agcctttttc
     3001 gtcgagccgg atcgatgttg ccgtagtcag tcctcgatct tcggtatcac atccatgatc
     3061 tcgtgggcag tcgcccggag gtgggggagg gcagcctgga tctcggcggc gcgggattgc
     3121 atgtcggtga ccgattggat gccgaggtcg gcgagcatgc cgcggaaggc cggaccgaag
     3181 cgtgtctcca gctccgggga gtcggcgtgg aggacctgca tgcaacgcgc ccaggtcaca
     3241 gcgatccaga acacggcttc ccggtggtcg ccggcggcga tcatcgaccg gctgccatcg
     3301 atggcgatcg ggcgggccgc cgggctcagg tcggaggcgg ggaacagcac cggcgtgcgg
     3361 atggccgaca ccgctgcgtc gaacgccggc tcgacggtgt cgagatggtg acggatccgt
     3421 tccggagaca gctccttgct gcccagcagg cccagcagct cctcatggac ggagggcatt
     3481 cctgtgctca caagcagctc ccgagcagcg atgtagcgct ttcggaccgt cgggttgcgg
     3541 agcccggcga ccagcagcac atgtgtggtg atgccggtgc cgaagagcca ggacatcgtg
     3601 ttctccgcga agggagcgga tgggccgatc cgtcccaggg agtcgccgat cttgccggca
     3661 gcggcttcga cacgtcgctc gacccacggc cgccgcgcga actcccgggc gatggtgtcg
     3721 cgcagagccc ccagtgtgcc gtctgggtcg gcgagcagct cgggcgagcg gaagctcggt
     3781 gcgagctggt ggttggcgag cacggattcg gggtcccgaa tgtctgccca cggcagcacg
     3841 gtgaggtcga gcagcacgcc ttcgaccggc tgcttgccgg gccgcaggtc ggcgggtggc
     3901 gtgtcaaaga ccaccatcag gtcgacgtcg ctggcgggat cgagcggcgc gtcatcggga
     3961 atggtcagga tcgagccgtg ccagagcgcg ccccggaagc cggggagagg cgcagcctgc
     4021 tcggcgagcc accgacgggc gatcgatcgt gcctcttcga cgtgcatggc gtgaaacctc
     4081 cgaacgacca gttgagcggt gggaccggct gtgctcgcag tacggtggag gccgatacgc
     4141 agtttatgca atcagccgca tcaccggcgc gttgtggaat ccgtacattg gccgatgtgc
     4201 tggtgcatgc gatccggcat gatgagcacg gttggatggg aacacccaag ggaggattcc
     4261 ggatttgcac cacccggaac cgttggtccg gccaatggcc gttgtgagag ttgtgccatg
     4321 atgtaccgtc atttctccaa ccgcgagcga tccgcgtttc gtgccctctc catcgcagtt
     4381 acgtcgttcc tgctcgccct gaccctgctt gtgccacccg cagcagctgg tcgcacaggt
     4441 cccctcgtcg acgaccgcag atcaggcgag agcggagcgc cggctgtcgt tgagcggtca
     4501 tgggatcaat accagatcga cttgctcgag gaccggaaac cgagcagcag cttcgacgga
     4561 ggcaactcca gcaactactg ctacgtctgc tacaagtacc gctagagtag acgacaggcg
     4621 acaggccctc tggtccgacc cggctcccgt taggagccgg gtccgtcgtg tgtcgcggca
     4681 ctgcctcacg gttttcgctt cgcggcgtga cccacaccgg tacggcccga tggtgcaccg
     4741 cttgacctag gagcgcacga ccggcatgat ggcaggcggg gcacggtgcc gtttggatac
     4801 gaaagcgatt ggttgtcgca ttgaccaacc tctctccgga tcaccggagc ctctccacct
     4861 ccatctgtat cccggcaagc aggcaggtgg gtggcacgtc gccgctccgc tgacgtcgat
     4921 cctcggccgt gatgcggaaa tcgatgccgt tgtcgcgcag ctggaccgtg gggaagtccg
     4981 gctggtgacg ctcaccggcc ctggagggat cggcaagact cggctcgtgc gggaagtcgc
     5041 gctgcgcatg caggagcact tcgcccacgg cgcctgcttt gtcgcccttg ccgccgtacc
     5101 ggatcccacc ttggtgcctt ccgcgatggc gagcgcgctc gggctccagc tcctgaccga
     5161 ccggcccgcc gaggagctcg tgtctgaggc gccgcgagat cgccacgtgc tgctggtgct
     5221 cgacaacctc gaacatctcg ccgagcaatc tgcaccatgg gtcgttgccc tgctcgagca
     5281 gtgcccgagg gtctcggcat tggcgaccag ccgcgtgcca ctgcgcattg ctggcgagca
     5341 tcgattcccg gtcgacccgt tggcactgcc cgaggaccga agcggacccg aagctcgcct
     5401 cgagaccatc gccgagagcg cggctgacga ctggcaacgt ggagggggtc gcgcagatct
     5461 gccgcatgct tgatggtctg cccctcgcga tcgaactcgc cgcggtgcaa gcgcgtctgc
     5521 tgcaacccgc gcagctgctg gagcggcttg gcgaccggct caccgaccta cgtactgacc
     5581 gacgggacgc gccaccccga caccagacca tgcgcgccgc gatggcctga gggtatgacc
     5641 tgctcggtcc ggacgcacag cgactctttc gctggctttc ggtattcgtc ggtgggtttt
     5701 cgttcaatgc ggcagaggcg atcgcaactg ctgcgggcgc cgacgacgcc cacgcaccat
     5761 tgctcgagtt ggttgagcac agcttcgtac gacgacgcga gcagggaccg gacggctacc
     5821 gattcagcat gctcgaggtc gtgcgggaat tcggtgccgg ccagctggag gctgccggcg
     5881 agctgacggc ggcacgggag gcccatgcgg cgtgggtgct ggagcttgca cgaaaggctg
     5941 agccgcggat ggccgggccg gagcaggtgg agtggctcga tcgcctcgcg caggagcatg
     6001 acaacatccg gcgggtggtc gggtggctga cggagcggga gcggatcgag gaggcgctcg
     6061 acctgaccgg ttcgctctgg ttcttccgct ggattcgtgg cttctatgac gaggcacgcc
     6121 ggcagttcga cattcttctg gcactccctg ctggtggaga ccgcacagtc gcgcgtgcga
     6181 aggcgcttat cggtctcggc atcgtcgcca gtcaccaggg cgacgtcccg cacgcgctgg
     6241 aggcgtttga ggaggcgatc gccatcctgc aggtgctcga cgagcagtgg catctctcac
     6301 tcgcgctggc ctgttatggg acggcttacc acatgatcgg gagaatcgat gaatgcgagc
     6361 ggcatctctc ggagagccag gcaaccgccg accggctcgg tgacgcctgg ctgtggcagg
     6421 gctcgatctg caacttcggt gtgattgctc tcagtcgcgg gcaatgggat cgcggccgtc
     6481 agtttatgga ggagtcggtg cgggcatcgc gatcgatagg tgaccgatgg ggactttcca
     6541 tcggcgagtt gaacctcggg tacatcgaca tgcatgaagg caatctcgac agcgcggagt
     6601 cgcacatcca gacgtctgtc agactgatcg aggagctcaa cgacaagcgc gatctcccgg
     6661 gcgcctactg ctcaatggcg gagattcttc gccagcgcgg ccagttggcc gaggcgaagg
     6721 caatgctcga cagcgccctg atggtcgcgc gagatatcgg cgaccgaacg cagatcgcca
     6781 acgcgctcac gggtttggcg catatcgttc atctccaagg cgagcaccgt gccgctgtcg
     6841 acctgatggg ggacgccatg cgcagtttcg agcgaggggg gcatctcgcc gcagcgttga
     6901 tgcgtctcga cgacattgcc gacatggcgc tggcgctggg ggatggcagg catgcggcgt
     6961 ggtgt
//
LOCUS       DASYWL010000150        10545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_570092, whole genome shotgun sequence.
ACCESSION   DASYWL010000150 DASYWL010000000
VERSION     DASYWL010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10545
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570092"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VGR22_05265"
     CDS             complement(<1..881)
                     /locus_tag="VGR22_05265"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2128012.1"
                     /translation="MLSYIFRRLVLLIPVILIVGLVVFMLVHLTPGDPAAVILGDQAT
                     PEDVQELRDQLGLNDPLPVQFVSWFTGVLRLDFGDSLFLGVPVTEALLDRVQPTGLLT
                     LYALIVQLVIGVPLGVISALKSGTVVDRLMTALAISGAAIPNFFLGIMLILVFAVTLQ
                     WLPSVGYVSPFEDPWEHIQRMFMPAFALGFSTAGLLARLIRSSMLDVLNEDYVRTAMA
                     KGLARRDVVIRHALRNALVPAITVVGTSIAALLGGAVVTETVFTIPGMGRLVVQSIGR
                     RDYPIIQGAVMAIAVTYV"
     gene            complement(1016..2647)
                     /locus_tag="VGR22_05270"
     CDS             complement(1016..2647)
                     /locus_tag="VGR22_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2128013.1"
                     /translation="MKTTRGKMTRRDVVQRSAGIGAGVAAAGMLGSVPGAMAAARRQD
                     EGEGNYGGTLRVGILGEPPTLDEHQTTANLVADVTYPMYETLFTYDENYEPVPELVEE
                     YTASEDGLTHTMKLREGVTFHNGEPMTAADVHASVTRWAQLSGLGGNLFERVNEFVEV
                     DEHTVEFRLSEPFGPLLTALAHNTQACTIHPKSILNEAGTEPLSRDEQFIGTGPYMLS
                     ERQADAYIRLTRYDDYVSRDEPVSGYAGAKHAYVDMIELIPVPDEAARVAGLQAGDYE
                     IAQEISNDQYEILAATPGVVAEIRPPAYEDMFFLNWHSPLMGNLALREAFRAALDHEA
                     IMIAARGGGEFTRLDPGWMMRETVWHTTAGEEHYNVNDPELARQKLEEAGYDGTPVRF
                     MTTQEYPYFYSASVIAQQQLEAVGFTIDLQVIDWATLIERRAQEDEWDVFVTYHGLVP
                     DPSQLTLVGQMSIYPGWWDSEESLALADQLLTEVDFDARYAVWEQIQEKIYTEIPAVK
                     IGDASIASYYSERIGGWIPMVERGIPYWNLWLEEE"
     gene            complement(3006..3818)
                     /locus_tag="VGR22_05275"
     CDS             complement(3006..3818)
                     /locus_tag="VGR22_05275"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEV2128014.1"
                     /translation="MLIRPPSGKKVHWQEMLRHELLEALEQRPVVIVPVGSVEQHGPH
                     CPMDVDISTPYHLAIRAAEAIDDFPVLVAPPVFYGFTHYNMGEVGTITLKLELFIELL
                     CDIARSLWANGFHRIILLNGHGGNQAPTWAASVKLAEEDVWALALTYWTMALDELREW
                     GEEEDDQGSIGHGGKWETSLQLYLRGELVAMDRAVKDEWRTKFSLEVQRYAKFPERRR
                     EMEHGVMGDPFMASAEKGERLFGVLLERLVALCREYHDEEPPRYREFGSHCP"
     gene            3999..5189
                     /locus_tag="VGR22_05280"
     CDS             3999..5189
                     /locus_tag="VGR22_05280"
                     /inference="COORDINATES: protein motif:HMM:NF015780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEV2128015.1"
                     /translation="MGFYDELGVTPVINAAGSLTRLGGNRVAPEVLDAMVEAAASNVV
                     MDELQQRAGAVIAEVTGAEAGYVVSGAAAGLTLAAAACVAGLDPAAMDRLPDTTGLRN
                     EIVVQRGHRNAYDHALRAAGITFVEAGYLGYPGAGGTYPWQIEAAITERTAAIACPIL
                     DTPGTLSLPEVAEIARRHDLPVIVDASAALPPRSNLRRFIAEGADLVVYSGGKAIGGP
                     QASGILAGRADLITSAALQHLDMDVRMPTWNRRNQVETGMLCGIPHHGIGRAMKVGRE
                     EIAGLIVALRRYVAGDDSADVRRWEGMLAHIAEPLEAIAGLEVHRELGTRQPVPLLAV
                     DIDADVVGFTAYDTANRLMAGDPRVAVAESRAELGTVTINPMALSDDEADLVRDRLQT
                     LFGA"
     gene            complement(5369..7000)
                     /locus_tag="VGR22_05285"
     CDS             complement(5369..7000)
                     /locus_tag="VGR22_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2128016.1"
                     /translation="MDTSRGKMTRRDVVQRSAGIGVGVATAGMFAGAPGMAAALRRQD
                     GGEGNYGGRLRVGIMGEPPTLDEHQTTAGIVAEVTYPMYETLFTYDANYQAIPMLVET
                     HTMSEDGLTHTMKLREGITFHNGEPMTAADVHASVTRWAQISGVGSNLFEAVDEFVEV
                     DEHTVEFRLSRPYGTILIALAHNTQACTIHPKAIMEEAGTEPLSRDEQLIGTGPYMLS
                     ARQADAYIRQVRFEDYVSRDEPINGYGGAKYAYVDEIEFIPVPDEAARVAGLQAGDYE
                     IALEISNDQYELLRETPGVVAQIQEPTNFEVFFLNWRSPLMSNLALRQAFQAALAHEP
                     ILTAARGGDPFTRLDPGWMMQQTTFHTTAGEEHYNVNDPELARQKLEEAGYDGTPVRF
                     MTTQEYPQFYSASVIAQQQLEAVGFTIDLQVIDWATLIERRAQEDEWDVFCTSHGFVP
                     DPSQITLVGQMGVYPGWYDSEESLALAEELLAEQDFETRYSIWEELQRNIYTEIPAIK
                     VGDAASASYFSERLGNWIEQVERGVPYWNLWLEDA"
     gene            7666..8901
                     /locus_tag="VGR22_05290"
     CDS             7666..8901
                     /locus_tag="VGR22_05290"
                     /inference="COORDINATES: protein motif:HMM:NF033206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HEV2128017.1"
                     /translation="MIRNTSRRTFVKSAAVMSAGLAAGLPASKSVFAQDATPITGAGV
                     GLPENAVVVASGLVNPRFIAVADDGTLYITENGIGGDEVIGQPEVGTAELEEGTPAAL
                     AVDATPVEAPPRPPSTRGFTGQITQVTPDGTQSVVASGLASYSDGAGPNGITIANGEV
                     YFDIGGGIAIEAGIDPLAGENSVHRLDPAAGTTELIAYLGQYEIDNNPDGTDVNPNLY
                     GMTTAAGGQLLVADAGGNTIYTVDPATGTFQLLAVVPPLGQLPGAEAAPAEQAERQPV
                     PTDVAVVDGSTLVGLLSEGWPEGAPSVLQLNPDGTFSAAGGALFFNVALGIGPDAQLY
                     ASELFGGFDESGMPAPGRVVRVYADGTVEPVVENVMTPHGINFDVAGNMYVVINSLMS
                     GPGMPAGQVIRVEGAAAPA"
     gene            complement(9020..9784)
                     /locus_tag="VGR22_05295"
     CDS             complement(9020..9784)
                     /locus_tag="VGR22_05295"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2128018.1"
                     /translation="MAVTRPEDETQVTFELDGTTYSTVAKRGQIIHEPFHHALPPELT
                     VGHRLTFAALNGDEFFADNLVGDVEDTYGEVRLITQAMAIAESKGEWKTIGFDHLAIT
                     LADRADARDFFRDVVGMQVMCDDPHLTVMATGPTALFLFDAGQDDAPLTTSQASQIHH
                     IGFIVDNLKHAYTHLQEHKDRLESDFTLLDRDERWSLYFFYRNGDVRFMIQFSEVKQA
                     ARSFTTPEKKAFADYLYDYTSRPYGLRFDASDTTDA"
     gene            9998..10408
                     /locus_tag="VGR22_05300"
     CDS             9998..10408
                     /locus_tag="VGR22_05300"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2128019.1"
                     /translation="MSQPIAAGAIHHLRLTVTDVERSRAFYTEVLNFEHVVDLPSGVF
                     LTNGAIGLGIGPSPDPSRAPADDRFDEARVGLDHLSFQVASRAELERAVQLLDERGIS
                     RGEITDLGDAFQIYILAFRDPDNIQLELTAAYGS"
BASE COUNT         1742 a         3348 c         3542 g         1913 t
ORIGIN      
        1 acgtaggtca cggcgatggc catcaccgca ccctgaatga tggggtagtc gcgacgcccg
       61 atgctctgca ccaccaatcg gcccatcccc ggaatggtga acacggtttc cgtcacgacc
      121 gcgccgccga gcagcgccgc gatcgatgtg ccgaccaccg tgattgccgg cacgagtgcg
      181 ttccgcagtg cgtggcggat cacgacatcg cggcgggcca gtcctttcgc catcgccgtg
      241 cggacatagt cctcgttcag cacatcgagc attgacgagc ggatgagtcg cgccagcaat
      301 cctgccgtgg agaatcccag cgcgaaggcg ggcatgaaca tgcgctggat gtgttcccat
      361 gggtcctcga acggcgagac gtagccgacc gacggcagcc actggagcgt caccgcgaac
      421 accaggatca gcatgatgcc aaggaagaag ttcggaatgg cggcgccgct gatggcaagc
      481 gcggtcatca ggcgatcgac gacggttccg ctcttgagcg cggaaatcac gccgagtggc
      541 accccgatga ccagttgcac gatcagggca tagagggtga gcagtccggt cggctgcacc
      601 cggtcgagca gtgcctcggt caccggcacg ccgaggaaga gcgagtctcc gaaatcgagg
      661 cgcaggacgc cggtgaacca ggagacgaac tggaccggca gagggtcgtt gagccccaac
      721 tggtccctga gctcctggac gtcttcgggt gttgcctgat cacccaggat gacggccgcc
      781 ggatcccccg gtgtgagatg gacgagcatg aacaccacca gcccgacgat gagaatcacc
      841 gggatgagga gcacgagtcg ccggaatata taggaaagca cgcaggctcc tctgtcatgt
      901 ccatcggcca gcgcacggca ggtatcgctt gcgcgctcta cacgactgcg ccatgacgtc
      961 cacgtgggga tccggaaatg ctctggatcc ccacgcaatc actgcgggtc tcgggctact
     1021 cttcctcgag ccagaggttc cagtacggaa tgccgcgctc gaccattggg atccagccgc
     1081 cgatccgctc cgagtagtaa ctggcgatgg aagcgtcacc gatcttgact gccggaatct
     1141 cggtgtagat cttctcctgg atctgctccc agacggcgta gcgcgcgtcg aagtcgacct
     1201 ccgtcagcag ctggtcggcg agcgccaagc tctcctcgga gtcccaccag ccgggataga
     1261 tgctcatttg gccgacgagc gtcagctggc tcggatcggg gacgaggccg tggtaggtca
     1321 cgaagacgtc ccactcgtcc tcctgggcac gccgttcgat cagggtcgcc cagtcgatca
     1381 cctgcaggtc gatggtgaag ccgaccgcct ccagctgttg ctgggcgatc acactggcgc
     1441 tgtagaagta cgggtactcc tgtgtggtca tgaaccgcac cggggtgcca tcgtagccgg
     1501 cctcctcgag cttctgccgc gccagctcgg ggtcgttgac gttgtagtgc tcctcgcccg
     1561 ccgtggtgtg ccacacggtc tcccgcatca tccagcccgg atcgagccgg gtgaactcgc
     1621 caccaccgcg ggcggcgatc atgatcgcct cgtgatccaa ggccgcccgg aaggcctcgc
     1681 ggagtgcgag gttgcccatc agcggcgagt gccagttgag gaagaacatg tcctcatagg
     1741 ccggtggtcg gatctctgcc accactcccg gagtggcggc caggatctcg tactgatcat
     1801 tcgagatttc ctgggcgatc tcgtagtcgc ccgcctgcag accggccacg cgggcggcct
     1861 cgtccggcac cgggatgagc tcgatcatgt cgacgtacgc gtgcttggcg ccggcgtagc
     1921 cgctgacagg ctcgtcacgc gatacgtagt cgtcgtaccg tgtgagacgg atgtaggcat
     1981 cggcttggcg ctcggagagc atgtacggcc cagtgccgat gaactgttcg tcacggctga
     2041 gcggctcggt gccggcctcg ttgaggatag acttcgggtg gatggtgcag gcctgcgtgt
     2101 tgtgggccag cgcggtcagc agcgggccga acggttcgct gaggcggaac tcgacagtat
     2161 gctcgtcgac ctcgacgaac tcgttgacgc gctcaaagag gttgccaccg aggccgctga
     2221 gctgcgccca gcgggtcacg gaggcgtgca cgtcggcggc cgtcatcggt tcaccgttgt
     2281 ggaaggtgac gccctcgcgc agcttcatcg tgtgggtgag gccgtcctca ctggcggtgt
     2341 actcctccac cagctccggc acgggctcgt agttctcgtc gtacgtgaag agcgtctcgt
     2401 acatcggata ggtgacgtcg gcgacgaggt ttgcggtggt ctggtgctcg tcgagggttg
     2461 gcggctcgcc gaggatcccg acacggaggg tcccaccgta gttgccttcg ccctcgtcct
     2521 ggcgtcgcgc cgcagccatc gcgcccggta cgctgcccag catgccggcg gccgcgacac
     2581 cggcgccgat gcctgccgat cgttgtacga catcgcgtcg tgtcatcttc ccgcgtgtgg
     2641 tcttcatcga gaaacctcct tgctgcgcca ctcaggaatc tgttaccgct tcgagcggga
     2701 gcgatcgctc tgcctcgctc gcaggttcgg atgtggcgac tggggcaatg atatctgagg
     2761 ctggatacct gtcaacacgt gatccgtgca agattgacgg acgtggctca gatgagccag
     2821 tgcacggcag aagtggcagg tatcacagcg gatagccatc atgtcacgct ccgagtgttg
     2881 gatctgtgtg agcaagagac gcttcgaccc attcgacttg ggccggtagc ccccgctcag
     2941 ggcaggaggg gttacagatc tgctgatgcc acgcggagca cactggcgcg ccatggccgc
     3001 acgcattacg ggcagtggga gccgaactcg cggtagcggg gaggctcctc gtcgtggtac
     3061 tcccggcaca gcgcgaccag ccgctccagc agcaccccga acagccgctc gcccttctcc
     3121 gccgatgcca tgaacgggtc gcccatcacc ccgtgctcca tctcccggcg ccgctcgggg
     3181 aacttcgcgt agcgctgcac ctcgaggctg aacttggtgc gccactcatc cttgacggcc
     3241 cggtccatgg cgaccagctc cccgcgcagg tagagctgca ggctggtctc ccacttgccg
     3301 ccgtggccga tgctgccctg atcgtcctcc tcctcgcccc actcccggag ctcgtccagc
     3361 gccatggtcc agtaggtcag cgccagcgcc cagacatcct cctcggccag cttcaccgaa
     3421 gccgcccacg tcggggcctg gttgccgcca tgcccgttga gcaggatgat gcggtgaaat
     3481 ccgttcgccc acaggctgcg ggcgatgtcg cacagcagtt cgatgaatag ctcgagcttg
     3541 agcgtgatgg tgccgacctc gcccatgttg tagtgcgtga agccatagaa gaccggcggc
     3601 gcgaccagca ccggaaagtc gtcgatcgcc tccgcggcgc ggatcgccag gtggtaaggc
     3661 gtggagatat cgacgtccat ggggcagtgg gggccgtgct gctcgaccga cccgacgggc
     3721 acgatcacga ccggccgctg ctcgagggcc tccagcagct cgtggcgcag catctcctgc
     3781 cagtgcacct tcttgccgga cggcggtctg atgagcatgg ccgctcctcc tctgtccccc
     3841 gtcgcgattg ctggtccttg cgtggcgggg tgtttcgtga atgatgtcgt ggaccgccgg
     3901 ttcagcggcc ggaccggtgg tcagatgcta cgggcggtcc gacgtggcgt caacgaggta
     3961 ggcaggctgg gggcgtccgc acggaacggg aggaagacat ggggttctac gacgagctgg
     4021 gggtgacgcc ggtgatcaac gcggccggat cactgacgcg gctggggggc aaccgcgtgg
     4081 cgccggaggt gctcgatgcg atggtcgagg cggccgcctc caacgtggtg atggacgagc
     4141 tgcagcagcg ggccggtgcg gtgatcgccg aggtaaccgg cgcagaggct ggctacgtcg
     4201 tgtccggcgc ggctgccggg ctcaccctcg cggcggcggc ctgcgttgcc gggctcgacc
     4261 ccgccgcgat ggaccggttg cccgacacca ccggactccg gaacgagatc gtggtgcagc
     4321 gcggccaccg caacgcctac gaccacgccc tgcgcgccgc cggtatcacc tttgtcgagg
     4381 ccggctacct cggctatccg ggcgcgggcg ggacctatcc atggcagatc gaggccgcca
     4441 tcaccgagcg caccgcggcg atcgcctgtc ccatcctcga tacccccggc acgctctccc
     4501 tgccggaggt cgccgagatc gcgcgccgac acgacctgcc ggtcatcgtc gatgcctccg
     4561 ccgcgctgcc cccacgcagc aacctgcggc gcttcatcgc cgagggagcc gacctcgttg
     4621 tctactcggg cgggaaggcg attggcgggc cgcaggcgag cggcatcctc gccggccggg
     4681 cggacctgat cacctccgcc gcgctgcaac acctcgacat ggacgtgcgg atgccgacct
     4741 ggaaccgccg caaccaggtg gaaacgggca tgctgtgcgg gatcccgcac cacgggatcg
     4801 gccgggcgat gaaggtgggc cgcgaggaga tcgccggact catcgtcgcg ctgcgccggt
     4861 atgtcgccgg ggacgattcg gccgatgtcc ggcgctggga ggggatgctg gcccacatcg
     4921 ccgagccgct cgaggcgatc gcgggactgg aggtgcatcg cgaactcggc acacgccaac
     4981 ccgtgccgct gctggcggtc gatatcgatg ccgatgtcgt ggggttcacg gcctacgata
     5041 ccgccaatcg gctgatggcg ggcgacccgc gggtcgcggt ggccgagtcc cgcgccgagc
     5101 tcggcaccgt caccatcaac ccgatggccc taagcgacga cgaggccgat ctcgttcggg
     5161 accggctgca gacgctgttc ggggcatgag caggggcgga cagtggggcc ccctgcgggt
     5221 cgattgcccc aacgattcat tcggattcaa gatgagagcg atgggagcaa tgaggccggc
     5281 cctgccaaac ggcagggccg gggatccttc gtcacgacgg cggcgctgat gcgccttgtc
     5341 ggctcaggat gacggggcct ggtgaacgct aggcgtcctc cagccacagg ttccagtagg
     5401 gcacaccgcg ctcgacctgc tcgatccagt tcccgaggcg ctcggagaag tagctggccg
     5461 atgccgcatc acccaccttg atcgccggga tttcggtgta gatgttgcgc tgcaattcct
     5521 cccagatgga gtagcgcgtt tcgaagtcct gctcggcgag caactcctcg gccaaagcca
     5581 ggctctcctc cgagtcgtac cagccggggt agacgcccat ctggcccaca agggtgatct
     5641 ggctcgggtc cggcacgaag ccatggctgg tgcagaagac gtcccactcg tcctcctggg
     5701 cacgccgctc gatcagggtc gcccagtcga tcacctgcag gtcgatggtg aagccgaccg
     5761 cctccagctg ttgctgggcg atgacgctgg cgctgtagaa ctgcgggtac tcctgtgtgg
     5821 tcatgaaccg caccggcgtg ccatcgtagc cggcctcctc gagcttctgc cgcgccagct
     5881 cggggtcgtt gacgttgtag tgctcctcgc ccgccgtggt gtggaacgtg gtctgctgca
     5941 tcatccaccc gggatcgagc cgggtgaagg ggtcgccgcc acgcgcggcc gtgagaatcg
     6001 gctcgtgggc cagggccgct tggaacgcct gcctgagcgc aaggttgctc atcagtggcg
     6061 agcgccagtt gaggaagaag acctcgaagt tggtcggctc ctgaatctgg gcgacgacgc
     6121 ccggcgtctc cctgagcagc tcgtactgat cgttggagat ttcgagcgcg atctcgtagt
     6181 cgcccgcctg caggccggcc acgcgcgcgg cctcgtccgg gaccgggatg aactcgatct
     6241 cgtcgacgta ggcgtacttg gcgccaccgt agccattgat gggctcatca cgcgacacat
     6301 agtcttcgaa gcgaacctgc cgaatgtagg cgtcggcctg gcgcgcggag agcatgtacg
     6361 gcccggtgcc gatgagttgc tcgtcccggc tcagtggttc ggtgccggct tcctccatga
     6421 tggccttcgg gtggatcgtg cacgcctgcg tgttgtgcgc gagtgcgatc agaatcgtgc
     6481 cgtagggccg gctgagccgg aactcgacag tgtgctcatc cacttcgacg aactcatcga
     6541 ccgcttcgaa caggtttgac cccacgccgc tgatctgtgc ccagcgcgtc acggaggcat
     6601 ggacgtcggc agcggtcatc ggctcgccat tgtggaaggt gattccttcc cgcagtttca
     6661 tcgtatgggt gagcccatcc tcgctcatcg tatgggtctc gacgagcatg gggatcgcct
     6721 gatagttggc gtcgtaggtg aagagcgtct cgtacatggg gtaggtgacc tcggcgacga
     6781 tgcccgccgt ggtctggtgc tcgtcgaggg tcggcggctc acccataatt ccgacgcgca
     6841 gccgcccgcc gtagttgcct tcacccccgt cctgacgacg cagggcggcg gccattcctg
     6901 gtgcgccggc gaacatgccg gctgtagcaa caccgacgcc gataccggct gatcgctgca
     6961 ctacgtcacg tcgtgtcatc ttgccgcggc ttgtgtccat ccgggacctc cctgatgcgc
     7021 cactcaacag aatctgttac cgatcgaggc tagcccacct gccatgaccc ctctacagat
     7081 ttgattgtgg caataggtgc gatcatatcc gggctcgctg aggtgtcaac ccccaatttc
     7141 tacacgccgt tctcgaaccg ttggcccgtt ggctcggctc tgctggcagg gccacggcca
     7201 gccgtgactg ctcgcggcgt tgcgttggct gtgcgagatg ggcgcggagc gggcgaccgc
     7261 ggtgttcgcc actgatacgg gcagtgaacc ggtacggcgg ctttatgaga cagtggggtt
     7321 tcgtggagag ctgcggtttg acgaccggga gtgcgacctg gccggccatg gtcagggcca
     7381 tccctgctgg ctggcacgcg gtgtgcgaca agattgtcgt cgtggcgaca ccggagatca
     7441 tgccatttcc ggtgcgcctg tgaaacgcct catcggtaga tgcgggattg cgtcttccat
     7501 tttcaggttc cgtccggttt gaccggccat gagttcggtg cgacggtcgg tgggcgacaa
     7561 acggtgagac agaccagttc ccgggctgtc gacaggtcgt gacgagcttt cggggctatt
     7621 tggttggatc ggtcccgtat cgactcgata tcaggagtgt ttagcatgat caggaatacc
     7681 tcacgtcgta cgttcgtgaa gtccgccgct gtcatgtctg ccggtcttgc cgccgggctg
     7741 ccggccagca agtcggtctt cgcccaggac gccaccccta tcacaggggc gggtgtcggg
     7801 ctgcccgaga acgcggtggt ggtggccagc gggctcgtca acccgcggtt catagcggtg
     7861 gccgacgacg gcacactcta catcaccgag aatggtatcg gcggtgatga agtaatcggc
     7921 cagccggaag tcggcacggc tgagcttgag gagggcacac cggctgcgct cgcggtggac
     7981 gcgacaccgg tcgaggcgcc gccgcggcca ccctccacac gtggcttcac cggccagatc
     8041 acgcaggtga cgccagacgg cacccagtcg gtggttgcca gcggtctcgc gtcatacagt
     8101 gacggtgccg gcccgaacgg gatcacgatc gcgaacggcg aggtctactt cgacatcggt
     8161 ggcggtatcg ccattgaggc aggcatcgat ccgttggcag gagagaactc cgtccaccgg
     8221 ctcgatcctg ccgccggcac gaccgagttg atcgcgtatc ttgggcagta tgagatcgac
     8281 aacaacccgg acggcactga tgtcaacccg aacctctacg ggatgaccac cgcggccggc
     8341 ggccagctgt tggttgccga cgctggcggg aacacgatct acacggtcga tccggccacg
     8401 ggcacattcc agctgctggc cgtggtgccg cctctgggcc agcttcccgg cgccgaggcc
     8461 gcgccagccg aacaggccga gcgccagccg gtgccgaccg atgttgcagt cgtcgacggc
     8521 agcaccttgg tcggtctgct gagcgagggc tggccggaag gcgcaccgtc ggtgctgcag
     8581 ctcaaccccg atggcacctt cagcgccgcc ggtggcgcgc tcttcttcaa cgtcgctctg
     8641 ggcatcggcc cggatgcgca gctgtacgcc agcgagctgt tcggcgggtt tgacgagtcg
     8701 ggcatgccgg caccgggccg cgtcgtgcgc gtctacgccg acggcacggt ggagccggtg
     8761 gtcgagaacg taatgacgcc gcatgggatc aacttcgacg tcgccggcaa catgtacgtg
     8821 gtgatcaaca gccttatgtc cggcccgggt atgcccgctg gccaggtgat tcgcgtcgag
     8881 ggggccgccg cccccgcgta atcccaggca gcgagccaga atgcgtggaa gaacaccccc
     8941 gcggccatgt ggccgcgggg gtgttgcgtg caggggacat cgaggtcgtc tgccagcagc
     9001 acgagacgac agtcgcgtgt cacgcgtcag tggtgtcgga tgcgtcgaac cggaggccgt
     9061 acggtcggct ggtgtagtcg tagaggtagt ccgcgaaggc cttcttctcc ggcgtcgtga
     9121 agctgcgcgc ggcctgcttg acctctgaga actggatcat gaaccgcacg tcgccgttgc
     9181 ggtagaagaa gtagaggctc cagcgctcgt cgcggtccag cagggtgaaa tcactctcca
     9241 gccggtcctt gtgctcctgc agatgggtgt aggcgtgctt caaattgtcg acaatgaacc
     9301 cgatgtggtg gatttgcgat gcctgactcg tcgtcagtgg ggcgtcatcc tgcccggcgt
     9361 cgaagaggaa gagcgccgtc gggccagtgg ccatcacggt cagatgtggg tcgtcgcaca
     9421 tcacctgcat cccgacgaca tcgcggaaga agtcgcgggc gtcggcacgg tcggccaagg
     9481 tgatcgcgag gtggtcgaag ccgatcgtct tccactcgcc cttggactcc gcgatcgcca
     9541 tcgcctgcgt gatgagccgg acctcgccgt aggtgtcctc gacgtcgccg actaagttgt
     9601 ccgcgaagaa ctcgtcaccg ttgagggcgg cgaaggtgag ccggtgtccg acggtgagtt
     9661 ctggcggcaa cgcgtggtgg aacggctcgt ggatgatctg tccgcgcttt gcgacggtgg
     9721 agtaggtggt gccgtcgagt tcgaaggtaa cctgcgtttc gtcttccggt cgggtaactg
     9781 ccatacctca gtgctccttg ttcatgaagg ccgtgcgagt cacgtcactg gattcatggg
     9841 cccatactgc agcatctgac gtccacgctt ctcgcgtgct catatccgaa taacctgctg
     9901 cgacagggtg gagaagcagc ggcctgctcg tgatgtatga tgcggatggg ttcccatctg
     9961 tgttgagatc gcgagtcata tgcaaaggag caaggccgtg tcccagccca tcgccgctgg
    10021 agcgatccat cacctccggc tgaccgtcac cgacgtggag cgcagccggg ccttctacac
    10081 agaggtgctg aacttcgagc acgtcgtgga tctgccgtcg ggcgtcttcc tgaccaatgg
    10141 ggcgattgga ttggggatcg gtcccagtcc cgatccgagt cgcgcgcccg cagacgatcg
    10201 cttcgacgaa gcccgcgtgg ggctcgacca tctcagcttt caggtcgcca gccgggccga
    10261 gctggagcgg gcagttcagc ttctggatga gcggggcatc tcgcggggcg agatcaccga
    10321 cctcggcgac gcgtttcaga tatacatcct ggccttccgc gacccggaca atatccagct
    10381 cgaattgacg gcggcttacg gctcatagct tggtggcggc tctcgacgcg gaggagcgca
    10441 cgaccgacaa gactggacat ccccaaatgc tgtagttgtc ctttggtgag gtcgaccgaa
    10501 tgttcgcaat cggcgcccct gctcgctcgc caggaagggc tatgg
//
LOCUS       DASYWL010000151        27670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_288308, whole genome shotgun sequence.
ACCESSION   DASYWL010000151 DASYWL010000000
VERSION     DASYWL010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 27670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27670
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288308"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /locus_tag="VGR22_05305"
     CDS             complement(<1..1117)
                     /locus_tag="VGR22_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEV2128020.1"
                     /translation="MEQFLPISTPSPARWWQAGVIYQVYPRSFQDTDGDGVGDLPGII
                     ERLDYLAGLGIDAIWVSPFYPSPMKDFGYDISDYRGVDPLFGTMEDAKRLIREAHDRD
                     LKVIIDYVPNHTSDQHSWFIESRSSRDNPKRDWYIWHDPAEDGGVPNNWLSEFGGNAW
                     EWDEQTEQYYLHSFLKAQPDLNWRNTDVRREMMDVLRFWLGLGVDGFRIDVLWKVIKD
                     DQFRDNPPNPNYDPDLPQSEKHLRVYSENRPEVHDVAALMRDTMEEYGHRVLVGEIYL
                     DLDELVSYYGENREGVHLPFNFSLILHPWDAAKVKDLILRYEDQLKKGMWPNWVLGNH
                     DQSRLVSRIGAEQAPIAAMLLLTLRGTPTIYYGEEIGM"
     gene            complement(1214..3292)
                     /locus_tag="VGR22_05310"
     CDS             complement(1214..3292)
                     /locus_tag="VGR22_05310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEV2128021.1"
                     /translation="MSAGAGIPKGSPSIAGLALAGLAGMVILVATWPSSWLSLGVVLV
                     AVGAAALVIARTRTDLTSLRPALECSGAASPTALLITAAALGTVATAVAVDNHLERGI
                     SNRAIAGIWLLSIAFFLLAAWLQSIVATVRRVPLRDVRQWRPRGLTVAAWLGIAAIAL
                     LPRLVWLDRFPTVLDGDEGSFMVRARLAQHGELADVFGPGFLGNPNLYPAVEGWLASI
                     VGAPPADYRILSALVGTLGVIATWRLGRYIVGPEAAAAGAIILATMPLNLHFSRSALN
                     NIADATTLAAALLFLIRGVVFRSRGDAALAGVALGLGFYGYFGGRVFPVVVLLSLAIM
                     TLTRRVRVREAARLGAWMTGGFLVTAMPLLTFYRSNPAEFGGRMNQVSLLSREGLEAG
                     SAAAIQLFVENLRDAMLYPLVSNNSGFFRRDPPYLGWSVGILLVIGLAVWLATWARRS
                     DPTAIAVLLVPWLVLTAGVALTVPIQGQRLVALTPLFALAGGSGLVAVASWLQAMVRP
                     PDARIGRLALAVALAVIGVSELRWFASEDRQIANYSDYRMTMMWDIGWRVDHAAPTSG
                     EPPAVLFAGPPFVFTGGFNNLVIQAPELEMSDVPEPIGPETNLALPEGTMLVIVAERE
                     GERCAAEALLPRSRVAEARARDGSLLYLALYREPLEGWSTAQTPAGSTFELVTASPCG
                     DPTDFDPVSR"
     gene            complement(3543..4385)
                     /locus_tag="VGR22_05315"
     CDS             complement(3543..4385)
                     /locus_tag="VGR22_05315"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2128022.1"
                     /translation="MSQTNGSQPLLEVRHLTKVFGSRETEVRAVDDVSFTVRQGEIVA
                     IVGESGSGKSTLARMLLRLSEPTSGEIIMDGRDVTEVKGSKNLKPYWREVQGIFQNPM
                     ASFNQFYSVGRVLRKTLALTGEKYSKDEEREKLSEALRIVGLDPKDTIPKGAHQLSGG
                     QLQRAMIARALLVDPKILIADESTSALDASLRVTILNVLKDLRDRLGMTVLFITHDIG
                     QANYIADRVFVMYQGEMVEQGTVDEVLGNPQHEYTQRLLADVPRLGGWGTEDESALEP
                     ASIG"
     gene            complement(4351..5385)
                     /locus_tag="VGR22_05320"
     CDS             complement(4351..5385)
                     /locus_tag="VGR22_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2128023.1"
                     /translation="MQVRDVRAYYETRHGPPIRAVDGVTLDLEHGEILGIVGESGCGK
                     STLAAVLSASGPPNLQIASGHLTLDDRETVDLARSGGTPREWRGNVVSLLPQRALNSL
                     NPTARIGDYAYDVVRAHNEKMRKREALELAGGRMEQLGLPSRVLDHYPHQLSGGMRQR
                     VVTVIATLLNPTVLIADEPTSALDVSSQKALILMLDKMMEEGMISRIIFITHDLALLS
                     NIANRVAVMYAGEIVEVGTTREVVRRPEHPYSKALIGSTLEPDPEQRGQVIYGLEGSS
                     PDMRNPPPGCRFHPRCPYVMDVCTHEVPPRMDPEHNYATCWWVKKEAEQGHETGGVNR
                     VTDERIAAAP"
     gene            complement(5397..6308)
                     /locus_tag="VGR22_05325"
     CDS             complement(5397..6308)
                     /locus_tag="VGR22_05325"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2128024.1"
                     /translation="MSTVFPSAAPDAALDPDAKSSILGRLHRLATQFSPRFWFALALT
                     LAVLFFGFVGPYLTGVEDPDQIVGGLYMDPSADAWLGTDNFGQDVFTQLIYGTRTSLI
                     IGIIAGLVATFIGVVIGISAGFYGGIVEEALMGVTNVVITIPSIVVLILLSIAIDSRS
                     IFAIAIIIGITSWPWTARAVRAQASSVRTREHVDLARLTGANTFSLMVFEVLPYMLSY
                     IVMAFVLQLTSSILQEAALSMLGLGPSDSISLGVMLFWALLWESIRTGAWWAFVPPAI
                     FLTLIAFALLLLQSSLDEVFNPRLRES"
     gene            complement(6318..7325)
                     /locus_tag="VGR22_05330"
     CDS             complement(6318..7325)
                     /locus_tag="VGR22_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2128025.1"
                     /translation="MSQFQFYVAKKAGWFLVAFVIALALNFYLPRMIPGNPVDAIVSQ
                     MAAGGADAASMEAIYETYIQEFGLDKPLLLQFFAYVGNVFQGDLGTSFALYPADVRGL
                     IADALPWTIALQLPAILVGWILGNALGAIAAYKGGWFDRGGFIASLFLSSMPYYAFAI
                     LLLYLVAVRWDLLPAAGGYSFGTEPSFSWSFITDAADHYWLPFLSLVLIFVGGQAIGM
                     RSMAIYELSSDYVEFSRGVGISDSRIVRYIFRNAMLPQVTGLALSIGSLVGGALITEV
                     VFSYPGIGSLLFGAIRQNDYPVIQGITLLIMATVLAANFLVDIAYGFIDPRIRAAQTG
                     T"
     gene            complement(7480..9225)
                     /locus_tag="VGR22_05335"
     CDS             complement(7480..9225)
                     /locus_tag="VGR22_05335"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2128026.1"
                     /translation="MSGRYTSSRRDVVKQGAALGSMAAIGAASFERFAPGASAAQLAR
                     NETLYVAGHQWGPATTFNPIAPDPAWPADSPFVYLYETLYTFNLLTGEIEPLLAADLP
                     EVTDEGMSITLHEETAFQDGEPLTAEDVVYTLTLPQRQDGIPYAIFNDYVSEVEAVDE
                     RTVRLTYNPDRVHPGMVRQLLTDIRILPQHIWEERETEGVTSVVEMEPVGSGPYQVTV
                     GSAQQVVLARWEDYWGADVVGMPKPKNIVHPIFESNDAGNLALQRGEIDFSQQFAPQI
                     WQMWQERDLPVGTWYPDPPYHVPGSIPMVHINLNRPGLDNPAVRRALAHAINYPQIAE
                     VAMSRYSITVNPSLLIPQGAEEQFFRADAIAESGGWEYNPDEARRILEEELGATLEDD
                     VYVLEDGTRLGPWRVVCPFGWTDWMTALELVAQGAQEVGFDITTEFPEAPVVTTAVQN
                     GDFDLILWSVAGVSAAGPWLRYRDVMDNRGVPEIGQTAFWNYNRFKSEEAEQLLDQAA
                     TAEGDELVDLYQQLDAIFRENIPTIPLMYRPLEFYEFNESVWQNFPTEDNPYAPPMHQ
                     SAGIQVLYGIESAGE"
     gene            9687..10328
                     /locus_tag="VGR22_05340"
     CDS             9687..10328
                     /locus_tag="VGR22_05340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2128027.1"
                     /translation="MADIPYDTVLERVRERYVDEPEIIQRLREETAELPNAGMLLGPE
                     DGALLAVMARTLNPERTLDIGVFTGFSALTVALNAPHARITALDVNDEITRRARRYWK
                     EARVADRIDLRIAPALDSLDALLHDGHQGSFDFAFIDADKPNYPHYVDRVTSLLRPGG
                     MMLLDNMLWSVLEEPQEDAELLDQLSRRMVADERLTTAVLPLGSGMTFAVRNR"
     gene            10396..10842
                     /locus_tag="VGR22_05345"
     CDS             10396..10842
                     /locus_tag="VGR22_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128028.1"
                     /translation="MGSRPPRHHTLPKSRPRQPRSVPNHQPAQTGLRMEAVAIFIGFA
                     IFPVALGASGVAYLARQEITDVATAVILTIVVGLVAAVFARSRRQWRRDARMSNELLL
                     AMLIGTIGVTFLIGGIAAMSSIWIVVGVAVLAGAILVGRTAKPTTR"
     gene            10927..11931
                     /locus_tag="VGR22_05350"
     CDS             10927..11931
                     /locus_tag="VGR22_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEV2128029.1"
                     /translation="MFTRTVPFPTDDDFAHAREVVSRYLTRTPLVPSRVRRTGWLKVE
                     SFQPTGSFKVRGALAALSAAPADRPIITASAGNHALGVAFAAKALDRNAMVVVPETAS
                     PAKIEALRTLPVQMITHGDDFDAAEAWAISRADAPAKDVTFLSPYNDPHVIAGQATML
                     DEIIAQHPGDRPLTVVAGAGGGGLLSGLGLGASQLTTDERPIRVVGVEAEASQGLSTA
                     VAAGQVEVVEVEPTLADGLAGNIEEGSITVELVKRHVAEIVQVTEDDIAAGIRWLVTN
                     HGLVAEGAGAAPVAALLADLIDPDLLDGDVVAILSGRNIALPILATVLMGEDVIHEAE
                     "
     gene            complement(12287..18442)
                     /locus_tag="VGR22_05355"
     CDS             complement(12287..18442)
                     /locus_tag="VGR22_05355"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HEV2128030.1"
                     /translation="MDLLAARSRSARTSARRDRITGAGWWLHVIGAIALMTGLFLPLT
                     AIAAPNGDPSALTRLQTGSDVVPPALTQVVAVGDFQSQLGCADFDLSCQPTQLTEHQG
                     IWTGVVAVPPGQYSWQIQVIAADGTVYTYGQGGLGGSPQQVTVGESDAGLYFRLNTHT
                     NEVEAASVSELYSVNVNGTAVALEPAGGQLTALVTVQQSDPATVQVFTGGEPLGAPQQ
                     ANLNQGPNRLTFSPDGQLVNVQPLAGASLTIERLDPNGNPLPGGCYQLRSGNTLLNQA
                     CDADDGADGYTTMTFPEGVPSGTVSVVEVTPPAGAEPVADQQIQLQPGDLFTTLQPEV
                     TQETTAEELATEEPAAEDGQIIEGEGEETAVPTEPEQAAPGDLLVYLSDQSGAPVPGA
                     CFQLLQNENVVAERCDRQPDDPAEFANNGTTGFFGVPAGTYTLRLSDAPNGATADDRR
                     VEVIAGQTVEVNVTAQLAATETPEPTATTQPTETPEPTATEAPQPANFTVTLLDQDGD
                     PVGGACWQLVHDGEVVAESCDTEAAESPEFADNGNTGFFDIQPGTYTLTMSAAPEGVT
                     PVEDRQVEIAPGSDDAETIRVQVAEPTEVVETPEPTTAVELSEPTQTATVEATETPTP
                     EVGPGNLTVTLVDGTQQPIGGACWQLLDDGTVVAESCDTSDWESAEFANNGNTGFFDV
                     PAGTYTLQMSSAPEGVSVDPREVTVEAGGDETVVVTANVSALPVTATQTATIEPTATV
                     EPTSTATATEETTTEAGPPANLIVELRTADGEPIGGACFQIAMEGQAPIVSCDADDPF
                     PGNGRTGFFGVPSGTYTLSQTTVPQGTNPIDPREITIEPGDNPELVVNPGEGDETPTE
                     EATGETQRTAGQQPAGTVTIDVTALGGEEICVQLDTGGGNGIANPPTACDNAEGDENP
                     EPGLIGFRNIEPGQYELSVISGVEAVQPQSITVAADGQTTVTVGEVPPTPTATSTATP
                     TATATATSTPTQTPTSTPTAEPTSTATPEPTPTATLTPTATATLTPTPEPTSTPTATV
                     TPTPEPTPTLTPTATPTPEPQTGSAQLALVSEDGLAVAGACIGLVNQDTGEELGPFCD
                     NGERDNDPTEGVILLRGIPAGVYEIVFTDVNAPAGASVLQVEEDREPIIIEILPGETI
                     EAEIVVPGLYILGSLEIRTIDAETGEPVPGACYTIGTDDPIEVCDGGDGDESPDVGTV
                     LITNLLPGEYVVTMSSPPEGYTGAESKTVTVTGGQIPTVRFEVQAEVTTGALTVAKVN
                     PAGEALSGACFRLQTDDETVAQACDGADGADDGTIRFVDIPVGSYELVETRAPQGFQA
                     GSARSVEITPGGDAEVEVVNTPTPGRLNVIKVAAKDPSTRLEGACFELRGEAIYGPFC
                     DDDDLNEDGRIVFGGVMPGTYTLVETQAPTGYDPASNREVTIRAGVTLQVTVQNQETP
                     PLPEAGTLVVNKLGPNNEALAGGCFRLYDGDTPVMGAVCDITDGTNDARIVFENVPAG
                     TWTLRETLAPSPAYQLAADQEVTIEDDETTEVDVVNRLKNGRILVRKVMPNGRPIEGA
                     CFELRPDQGDPLCANADGELLFEDLPVGTYTLVETTVPYGFRESGPVENIEVHPAQTT
                     IVTVENELQPPPNTGSVQVHKFVCLVDSADEERTNFLGGGAGNAELAKTKGCERGDAE
                     FTLVGEDGSDGPGTFRTGDDGRYQVTLPAKIYRLDETNPDLPGNSAALLRVEPGRMTT
                     VIVINYVAPPEPEPVDIHVTKYTCQAGFSGTTFADFAASCMQSSQLTNNITVRAEGPV
                     DLKTVTGEGGQVGRAVFADQPSGTWTVYEERPYNIPTSYQFCGWDPNSPTAFKTINGA
                     ITADLGEGENLYCVLFNIPEPVTDSTGVILVRKYVCDVEDPPKGYDFESECRLSDQLS
                     TFELKRFNEEMQDYGDGVQQTANPDGILRFVDLRPGTFQLREVSGNWCYANSNSVNAK
                     GDVVVKAGQVSLVSIYNCVGPSQPPNTGSGDAADLLDSTPEPGPPAPGEPRVTPGVAW
                     PLAAMAAVVFALRPRRVLAPGHDWRVDDALDRDVA"
     gene            complement(18982..20085)
                     /locus_tag="VGR22_05360"
     CDS             complement(18982..20085)
                     /locus_tag="VGR22_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV2128031.1"
                     /translation="MPGERLRFAVDRSHYEVAVAQGLGERPDAAIEGVVRLAKAADAA
                     GVDSFWLSEDPDGWDAIAMLGAIARETVRIRLGPGVLNPYFRHPSLIAASMSTLDALA
                     GGRAFLGFGRGQTEWYERALGMEVGWPVTALEESFQLLRQWLTPPWTAEAAADASAFR
                     VTRWERVTGPVQEHLPMYLAAVGPKAMSLAARYADGVIFNDLSSMTFMEAAIRDVRRD
                     AAEQGRDAEALRFFARAAVTITDDPEAVWQRRKATVALIHALPGMERLLESPGFDTAA
                     IIAEVRTAMRTMEVLERGGNFPELKQAGDMAAARRAIPDDLMRELVVAGPMGEVWERL
                     RQFREIGITDVFLAPPTGDPDELTDIVAALVDA"
     gene            complement(20085..20282)
                     /locus_tag="VGR22_05365"
     CDS             complement(20085..20282)
                     /locus_tag="VGR22_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128032.1"
                     /translation="MRPVEGQGQLWYCGKHGMYGWLIEKDEAENAERGSERELPNGVT
                     GVVMGTGDERQGGVVLYLREA"
     gene            20495..21073
                     /locus_tag="VGR22_05370"
     CDS             20495..21073
                     /locus_tag="VGR22_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128033.1"
                     /translation="MRIAVLLLALTLLPAVPAAAQEGTVTPAAGPSLADVAPLDILQA
                     LEGADAPETLPGNTDEEIVLQTWEEHYGERLNTTLGAWVMTGSTQLPMASVLVFPSAE
                     NAVAGLGEFRRDSSATTAGTLEAWTIADRGKWICVAVDGPVMLIGQAEPEVGEAEETV
                     RERSCEVTAATHEWMLETVGLTGGDATPAADG"
     gene            complement(21538..22338)
                     /locus_tag="VGR22_05375"
     CDS             complement(21538..22338)
                     /locus_tag="VGR22_05375"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEV2128034.1"
                     /translation="MQRVFRVVDTADLRPMDVKPTARMVRSVHDHGILVPVLLRETVD
                     EDGVIALEIVDGNRRVTAAVEVGLERVPAVVLQHVGPEAVSAMTVIANTLRSRNAVSE
                     WQAIERLGDLGHDVEGIMRLTGLTKASLETRLRPSEMHPDLRRAMLEGRLGVTIWERA
                     GRLPPEAQQRLADLLEERGGLRMADVDAERRRVEAEETVRDDVELPPATEQPGTREQL
                     ADRLAELAAFAGAIGMSEYEWSRLATGAWNRRTEAGERPVEGEGLTPE"
     gene            complement(22593..23717)
                     /locus_tag="VGR22_05380"
     CDS             complement(22593..23717)
                     /locus_tag="VGR22_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HEV2128035.1"
                     /translation="MTWIVIPAHLDALEIGAGILGTGGGGNPYLGKLIARKFVEEGAQ
                     VAIVDPNEVPDDAVVASVGGMGSPTVSIERLRRADESVKALRALEAHIGKRVTHLVPS
                     EIGGSNSTVPMVVAAQVGLPVIDGDGMGRAFPELQMETFTMYGVTPTPAALCNHLGHI
                     AIFNRIDDATTLERYARAVTIQMGGSAGYAFPVMTGSQLKKTVIAGTLSLAIRLGEAV
                     MSARREKTDPVVAVCEVTGGQRLFAGKVTDVERRMTGGFARGVVVMEGFGNDAGRTLR
                     VDFQNENLIARDDAGAVLAVVPDLICLIDSDTGAPLTTEVLRYGLRATVIGIPAPEEL
                     RTEVGLRFVGPAAFGYEDVTYTPLRGSYANREQTCNRYSG"
     gene            24040..25413
                     /locus_tag="VGR22_05385"
     CDS             24040..25413
                     /locus_tag="VGR22_05385"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2128036.1"
                     /translation="MPAPMTTDPEIAVLLAEIRGGIAATLGNDLVGLYLFGSLVSGDF
                     TPGVSDIDLLAVSQRFMTDPDIDRLRDMHREFVERHPEWEDRIEVAYQSKHGLRTWQT
                     ERSPMGIISPGEPIHLIEAGAEWRINWFFVLDHGVTLAGPPPETLIAPITREVFIEAA
                     RELGRSWIDRIHTVTDQCGESYAILTVCRALYSHQAGELVSKQQAARWVQARRPEWSE
                     LIEMALERRVSPNDNGPDDSFDDTVRFIETMAAELDGDNAPDASASTRARRRGFATQD
                     DSRVSIAKWRLEPAMPGDFDILFDIHRRAMGLYVEAIWGWDDGDQRRRFRGALKPGVQ
                     AVVVDGAIVGMLDVRRGDDEVFIGNIELAPEMQGRGIGSAIIRSVLAEAQGHGVPVRL
                     HVFKINSRARRLYDSLGFSVVGETELHYEMLWRTAEGTRANRVDPDRGQGTDRRYAPG
                     DSSLRSE"
     gene            25520..27064
                     /locus_tag="VGR22_05390"
     CDS             25520..27064
                     /locus_tag="VGR22_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEV2128037.1"
                     /translation="MLRIGIDVGGTNTDAALMQGSTVHATCKTPTTPDITTGIVSAVS
                     TILVSGRVAAENISSVMIGTTHFTNAVIERRHLAPTAVVRLALPAAESIPPMMDWPTD
                     IRDAVGNTAFMVHGGNEFDGREIHPLVPEELEAVASAIRDAGITDIAISAAFAPVRPA
                     MEDRAAAILGELLPHASITTSHEVGQLGLLERENAAILNAALRPLARKTVAAFREAIT
                     GMGMTAPLYLTQNDGTLMTADFAERFPVLTFASGPSNSVRGAAFLSGRKNALVLDVGG
                     TTSDLGVLVDGFPRRSSLAVDVGGVRTNFRMPDVYSFGLGGGSIVTCERDEAQVGPRS
                     VGYRITEKARVFGGETLTATDIAVASGWTDLGDRSRVTDLDPCLLQVAQRRIREMVSA
                     GVDRMKTQAGEVDLIVVGGGSILVTEVNGVGDLIRPAHHDAANAVGAAIAQISGEVDR
                     VFALDEMTRDEAVTVAKREATERAVTAGADPATIEIVDVEDIPLAYLPSNATRIRAKA
                     VGDVRE"
     gene            27261..>27670
                     /locus_tag="VGR22_05395"
     CDS             27261..>27670
                     /locus_tag="VGR22_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128038.1"
                     /translation="MEDIISASPWATLVEQLRRGTMSRREFVRLETAAGISVGAASHT
                     ARQTGAQATALEAGQATGATSSTGAAEPGGRSINREELDQIIRDTFDLSEPEVIGGQV
                     IYGEISDIDTLNPHLHVDIYSSYLTANMFEYLATT"
BASE COUNT         4696 a         9458 c         8842 g         4674 t
ORIGIN      
        1 gcatgccgat ctcctccccg tagtagatcg tcggcgtgcc gcgcagcgtg agcagcagca
       61 tcgccgcgat cggagcctgc tcggcgccga tcctgctcac cagccgggac tggtcatggt
      121 tgcccagcac ccagttcggc cacatgccct tcttcagctg atcttcgtag cgcaggatca
      181 gatccttgac cttcgctgca tcccatgggt ggaggatcag cgaaaagttg aacggcagat
      241 gcacgccttc gcggttctcg ccgtagtagc tcaccagctc gtcgagatcg agatagatct
      301 cgccgaccag cacccgatgc ccgtactcct ccatggtgtc gcgcatcagg gccgcgacat
      361 catgcacctc gggccggttc tcgctgtaga ccctgaggtg tttctcggac tggggcaggt
      421 ccgggtcata gttcgggttc ggtgggttgt cccggaactg atcgtccttg atcaccttcc
      481 agagcacgtc gatgcggaag ccgtcgacac cgaggcccag ccagaagcgc agtacgtcca
      541 tcatctcgcg tcggacatcg gtgttgcgcc agttgaggtc cggctgcgcc ttgaggaagc
      601 tgtgcaggta gtactgctcg gtctgctcgt cccactccca ggcgttgccg ccgaactccg
      661 acagccagtt gttgggcacg ccgccgtcct cggccggatc gtgccagatg taccagtccc
      721 gcttcgggtt gtctcgcgac gaccgggact cgatgaacca ggaatgctga tcggacgtgt
      781 ggttcggcac gtagtcgatg atcaccttga ggtcgcggtc gtgcgcctcc cggatcagcc
      841 gcttcgcgtc ctccatcgtg ccgaacagcg gatcaacccc acggtagtca ctgatgtcgt
      901 agccaaagtc cttcatcggc gacgggtaga agggcgacac ccagatggcg tcgataccaa
      961 gccctgccag atagtcgagg cgctcgatga tgcccggcag gtccccgact ccgtcaccgt
     1021 ccgtgtcctg aaacgagcgc gggtagacct ggtagatcac gccggcttgc caccacctcg
     1081 cgggtgatgg cgtcgatatc ggcagaaact gctccatgga cctcgatcct tcgtgaatgc
     1141 ctcggattga tccctcgggc caccacggcc cgggtgcctc ccgccattcc gcaagaaccg
     1201 ggcacgggcg ccactatcga ctgactggat cgaaatccgt cgggtccccg caaggcgaag
     1261 cggtgacgag ctcgaacgtc gagccggcgg gcgtctgggc ggtcgaccat ccctccagcg
     1321 gctcccggta gagcgcgagg tagagcagcg atccgtcccg tgcccgcgcc tcggccaccc
     1381 gcgatctcgg cagcagcgcc tccgccgcgc accgctcccc ctcccgctcc gcgacgatca
     1441 cgagcatcgt cccctccggc agggccaggt tcgtctccgg cccgatcggc tccggaacgt
     1501 ccgacatctc cagctccggc gcctggatca ccagattgtt gaatcccccg gtgaagacga
     1561 atggcggccc ggcgaacaac accgccggcg gttccccgct ggtgggcgcg gcgtgatcaa
     1621 cccgccagcc gatgtcccac atcatcgtca tgcgatagtc gctgtagttg gcgatctgcc
     1681 ggtcctccga ggcgaaccac cgaagctcgc tgaccccgat caccgcgagc gccaccgcaa
     1741 gggcgagccg accgattcgc gcgtcgggtg ggcgcaccat tgcctgcagc caggaggcga
     1801 ccgccaccag cccggagccg cccgcgagcg cgaagagcgg cgtcaacgcc acgagccgct
     1861 gtccctggat cggtacggtc agcgcgacac ccgccgtcag cacgagccac ggcacgagca
     1921 gcaccgctat ggcggtcggg tcggaccgcc gcgcccatgt ggcgagccac accgccagcc
     1981 cgatcaccag caggatgcca accgaccatc ccagatatgg cgggtcacga cggaagaacc
     2041 cggagttgtt gctcaccagg gggtagagca tcgcgtcccg gaggttctcg acgaaaagct
     2101 ggatcgcggc agccgagccg gcctccagcc cctcgcggga caacagcgac acctggttca
     2161 tccggccacc gaactcggca ggattggatc gatagaacgt gagcaacggc atcgcggtca
     2221 ccaggaagcc gcccgtcatc caggcaccga ggcgagcagc ctcgcgcact cgcacgcgtc
     2281 gcgtgagcgt catgatcgcc agcgagagca gcaccacgac ggggaagacg cggccgccaa
     2341 agtaaccgta aaagcccagt cccagcgcca cccccgccag cgccgcatca ccacggctcc
     2401 tgaacacgac accgcggatc aggaagagca gcgcggcagc caacgtggtc gcatcagcga
     2461 tgttgttgag cgcggagcgg ctgaagtgca ggttgagggg catcgtcgcc aggatgatcg
     2521 ctccggccgc cgctgcctcc ggcccgacga tgtatcggcc gagccgccaa gtcgcgatca
     2581 ccccgagcgt gccgacgaga gcgctcagga tccgatagtc cgctggcggt gctccgacaa
     2641 tgctggcgag ccagccctcc acagcggggt agaggttggg attgccgaga aagccgggtc
     2701 cgaacacgtc cgcaagctcc ccatgctggg caagcctggc gcgtaccatg aaggaaccct
     2761 cgtcgccgtc gagcacggtt ggaaaccgat cgagccacac gaggcgcggc agcagcgcga
     2821 tggcggcaat ccccagccac gccgcgactg tcagtccccg gggacgccac tgccggacat
     2881 cacgtagcgg cacgcgccgc accgtcgcta cgattgactg caaccatgcc gcgagcagga
     2941 agaacgcgat ggagaggagc cagattcctg cgatggcacg attggagatc ccgcgctcga
     3001 gatggttgtc gaccgcgacc gccgtggcaa cggtcccgag tgctgccgct gtgatgagca
     3061 gggcggtagg ggacgccgca cctgagcact ccagcgctgg acgcagcgag gtcagatccg
     3121 tcctcgtcct ggcgatcaca agggcggccg ctccgaccgc aaccaggaca accccgagcg
     3181 acagccacga cgacggccaa gtcgcaacga gtatcaccat gccggcgagc cccgccaggg
     3241 ccagaccggc aatcgacggc gagcctttcg ggatgccagc gcctgcactc atgccacatt
     3301 caccgagaga gcgaactgcc cggtacgatg tccgcattgt agcaacgccc cgcacggtcc
     3361 cgatcgatgt cggtagctat cgcacaagcg acttgactgc cgggtcgttg ttcctgctct
     3421 ctcgacacct gatcgggccg cgggcaggaa ccgtgccggt gtgctgttgt atccggcacg
     3481 ccggcatggt gcatatcctc catgccggcg tgtgggtcga ttcaggtaaa ttgggagccg
     3541 aatcagccga tgctggctgg ctcgagagcc gattcatcct cggtgcccca gccgcccaat
     3601 cgcgggacat cggccagcag ccgctgggtg tactcgtgct gcgggttgcc gagcacctcg
     3661 tcgaccgtcc cctgctcgac catctcaccc tggtacatca cgaacacccg gtcggcgatg
     3721 tagttcgcct gcccgatgtc gtgtgtgatg aagagcaccg tcatcccaag ccgatcgcgc
     3781 aggtctttca gcacgttgag gattgtcacc cgcaacgagg cgtccaacgc cgaggttgac
     3841 tcgtcagcga tcaggatctt cggatccacc agcagcgccc gcgcgatcat cgcacgctgc
     3901 agctgcccac cggagagctg gtgggccccc ttggggatcg tgtccttcgg gtccagacca
     3961 acgattctca gcgcctcgga gagcttctcg cgctcctcgt ccttgctgta cttctcaccg
     4021 gtgagcgcga gcgtctttcg cagcacccgc cccactgagt agaactggtt gaaggacgcc
     4081 atcgggttct ggaagattcc ctgcacctcg cgccaatacg gcttcaggtt cttcgagccc
     4141 ttgacctcgg tgacatccct gccgtccatg atgatctcgc cggaggttgg ctcggagagc
     4201 cgcagcagca tgcgcgccag cgttgacttg ccgctgccgc tctcaccgac gatcgcgacg
     4261 atctcgccct gcctgaccgt gaacgacacg tcgtccacgg cgcgcacctc cgtttcgcgg
     4321 gagccgaaca ccttggtcag gtggcggacc tcaaggagcg gctgcgatcc gttcgtctgt
     4381 gacacggttg actccgccgg tctcatggcc ttgctccgcc tccttcttca cccaccaaca
     4441 ggtcgcgtag ttgtgctccg gatccatcct cgggggcacc tcatgggtgc agacgtccat
     4501 cacgtacggg cagcgcgggt ggaagcggca acccggaggc gggttccgca tgtccggcga
     4561 actgccctcc aggccgtaga tgacctgccc gcgctgctcg gggtcgggct ccagcgtgga
     4621 gccgatcagt gccttggaat agggatgctc cggccgacgc acgacctcgc gcgtggtgcc
     4681 cacctccaca atctccccgg catacatcac cgccacacga ttggcgatgt tgctcagcag
     4741 cgccaggtcg tgcgtgatga agatgatgcg ggagatcatc ccctcctcca tcatcttgtc
     4801 cagcatcagg atcagcgcct tctgggagga gacgtcgagc gctgaggttg gttcgtcggc
     4861 gatcagcacg gtcgggttga ggagggtcgc gatcaccgtc accacgcgct gccgcatgcc
     4921 gccgctgagt tggtgcgggt agtggtccag aacgcgcgat ggcagcccga gctgctccat
     4981 ccgtccccca gccagctcca gtgcctcacg cttgcgcatc ttctcgttgt gggcacggac
     5041 tacgtcatag gcgtagtctc caatgcgcgc ggtggggttg agcgagttga gcgcgcgctg
     5101 gggcagtagc gagaccacgt tgccgcgcca ctcgcgcggc gtcccgccgg accgtgccag
     5161 atcgaccgtc tcccggtcgt caagggtcag gtgcccgctc gcgatctgga gattcggcgg
     5221 cccgctcgcc gagagcaccg cggcgagggt cgacttgcca caccccgact caccgacaat
     5281 gccgaggatc tcgccgtgtt cgagatcgag cgtgaccccg tcgacggccc ggattggtgg
     5341 cccatggcgt gtttcgtagt aggcgcggac atcccggact tgcatggacc ctctccctag
     5401 ctctcgcgta ggcgcggatt gaagacttca tcgagactgg actgcagcag cagcagcgcg
     5461 aacgcgatca gcgtcaggaa gatcgccggc ggcacgaacg cccaccacgc acctgtccgg
     5521 atcgactccc acagcagcgc ccagaagagc atgaccccga gcgagatcga atccgacggg
     5581 ccgagaccga gcatgctcag cgccgcctcc tgcaggatgc tgctggtcag ttggagcacg
     5641 aacgccatca cgatgtagga gagcatgtac ggcagcacct cgaacaccat cagcgagaag
     5701 gtgttggcac cggtgagccg cgccaggtcg acgtgctcgc gcgtgcgcac cgagctcgcc
     5761 tgcgcgcgca cggcacgggc agtccacggc cagctcgtga tcccgatgat gatggcgatg
     5821 gcgaagatcg agcgcgaatc gatggcgatc gagagcagga tcagcaccac gatcgagggg
     5881 atggtgatca ccacattggt aacgcccatc agtgcctctt caacgatgcc accatagaac
     5941 ccggcggaga tgccgatcac gacaccgatg aacgtcgcaa ccaggcccgc aataatgccg
     6001 atgatgagtg acgttcgggt gccgtagatg agctgggtga agacatcctg accgaagtta
     6061 tcggttccca gccaggcgtc ggccgacgga tccatgtaga gcccacccac gatctggtcc
     6121 gggtcctcaa caccagtcag gtacggaccc acgaagccga agaacagcac ggccagggtg
     6181 agtgcgagcg cgaaccagaa acggggtgag aactgggtgg ccagccggtg cagccgcccg
     6241 agaatggagc tcttcgcatc cgggtcgagt gcggcgtctg gtgccgctga gggaaagacg
     6301 gtactcatgg tatgctccta ggttccggtc tgcgcggcgc ggatgcgcgg gtcgatgaaa
     6361 ccgtaggcga tatcaaccag gaagttcgct gccagcaccg tcgccatgat cagcagcgtg
     6421 atgccttgga tcacggggta gtcgttctgc cggatggcac caaagagcag gctgccgatg
     6481 ccgggatagg agaagaccac ctcggtgatc agcgcgccac ccaccagcga gccgatcgag
     6541 agcgccaggc cggtcacctg cggcagcatc gcgttgcgga agatataccg cacgatgcga
     6601 ctgtcggaga tcccgactcc gcggctgaac tccacgtaat cggagctgag ctcgtagatc
     6661 gccatcgatc gcatgccgat tgcctgcccg ccgacgaaga tgagcacgag cgacaggaac
     6721 ggcagccagt agtggtccgc agcgtcggtg atgaacgacc agctgaagct gggctcggtg
     6781 ccgaaggagt agccgccggc ggccggcagc aggtcccagc ggacggcgac caggtagagc
     6841 aggaggatgg cgaaggcgta gtagggcatg ctggagagga agagcgaggc aatgaaccca
     6901 cctcgatcga accagccgcc cttgtaggcg gcgatcgcac cgagcgcgtt gcccagaatc
     6961 cagccgacca ggatcgccgg gagttgaagg gcgatcgtcc acggcagggc atcggcgatc
     7021 agtccacgca cgtccgctgg atagagcgcg aacgaggtgc ccaggtcacc ctggaagacg
     7081 ttgccgacgt aggcgaagaa ctgcagcagc agcggtttat ccagcccgaa ctcctggatg
     7141 tacgtctcgt agattgcctc catcgacgcc gcatccgctc cccccgccgc catctgggag
     7201 acgatggcat cgacgggatt cccaggaatc atgcggggca ggtagaagtt gagcgcaagc
     7261 gcgatgacga acgcgaccag gaaccatcca gctttcttcg cgacatagaa ttgaaactgc
     7321 gacatctcgt gcccccaaaa tcctggagtt tgtcacaatg gcccgtatat gaggcggatg
     7381 acgtcgcgac aggagactgt cgttcagcta tggtgtgccg gagtgaggaa aggggccggg
     7441 caacgccgcc ccaccccttt ccgtacacga ttcctcctgt tactccccgg cggactcgat
     7501 gccgtagagg acctggatac ccgcgctctg gtgcatcggc ggggcgtatg ggttgtcctc
     7561 ggtcgggaag ttctgccaga ccgactcgtt gaactcgtag aactccaacg gccggtacat
     7621 cagcgggatg gtcgggatgt tctcgcggaa gatcgcgtcg agctgctggt agagatcgac
     7681 aagttcgtcg ccctcagcag tcgccgcctg gtcgagcagc tgctcggcct cctcgctctt
     7741 gaaccggttg tagttccaga aggccgtctg cccgatctcc ggcacgccac gattgtccat
     7801 gacgtcgcgg taccgcaacc acggcccggc ggcggagacg ccagcgaccg accagagaat
     7861 gagatcgaag tcgccgttct gcaccgcggt ggtcaccacc ggcgcctcgg ggaactcggt
     7921 cgtgatatcg aagccgacct cctgcgcgcc ctgcgcgacc agctccagcg ccgtcatcca
     7981 gtcggtccag ccgaaggggc agacaaccct ccagggaccg agccgtgtgc cgtcctcgag
     8041 cacatagaca tcgtcttcca gcgtcgcgcc gagctcctcc tcgagaatcc gcctggcctc
     8101 gtccgggttg tactcccaac cgccggactc ggcaattgcg tcggcccgga agaactgctc
     8161 ctcggcgccc tgagggataa ggaggctcgg gttgaccgtg atggagtacc gcgacatcgc
     8221 gacttcggcg atctgcgggt agttgatcgc atgggccagc gcccgccgca cggcaggatt
     8281 atcgagcccg ggccggttga ggttgatgtg caccatcggg atggatcccg gcacgtggta
     8341 cggcgggtcg gggtaccagg tgccgaccgg caggtcacgc tcctgccaca tctgccagat
     8401 ctgcggcgcg aattgctgcg agaagtcgat ctcgccgcgc tgcagcgcga ggttgccggc
     8461 atcgttggac tcgaagatcg ggtggacgat gttcttcggc ttcggcatgc caaccacgtc
     8521 cgcgccccag tagtcctccc accgggccag gaccacctgt tgcgccgacc cgactgtgac
     8581 ctggtaggga ccggagccga ccggctccat ctctaccacg ctggtgaccc cctcggtctc
     8641 gcgctcctcc cagatgtgct gcggcaggat ccggatatcg gtcagcagtt gccgcaccat
     8701 gccggggtga acacgatcgg ggttgtaggt gagccgtacc gtgcgctcgt cgaccgcttc
     8761 gacctcggag acgtaatcat tgaaaatggc gtaggggata ccgtcctgcc gctgcgggag
     8821 cgtcagcgtg tagacgacat cctcggcggt gagcggctca ccatcctgga aggccgtctc
     8881 ctcgtggagg gtgatgctca tcccctcgtc tgtcacctcc ggcaggtcgg ccgccagcag
     8941 cggctcgatc tcgccggtga gcaggttgaa ggtgtagagc gtctcgtaga ggtaaacgaa
     9001 cggcgaatcc gccggccagg ctggatccgg cgcgatggga ttgaacgtgg tcgccggacc
     9061 ccactggtgg ccggcgacgt agagcgtctc gttgcgggcg agctgcgcgg cggatgcacc
     9121 gggggcgaag cgctcgaatg acgcggcgcc gatggcagcc atcgatccca gcgcagcacc
     9181 ctgtttgacg acatcgcggc gagaggatgt gtaacgacca gacatgttca ctgaacctcc
     9241 tgtagttacg ccgtacgcct cgatgatgat gtgcccggac atcgactcct gccgaatcac
     9301 ggccaggcgc tatgcatcat gtggtacttg gctgtgatga tagcacaaac atatcaatag
     9361 cgggagcgct cccacaattg ggtcgggtac ccacgccgca tgccccctct ccgatcgtgc
     9421 gctagcatgg agataccgat acggggcagc gggcagtcac caggagaggc acaggcagtg
     9481 gacccggtcg aattcatctg ctcttcgcgc tcatcgctgc ggtggccctg cccggcgtcg
     9541 tgatctggtg gctcgcccac cgcagccgca ccaccacgac gcacgagagc agattccccc
     9601 gtcgcgaccg ctcctaacag cacgagacga tctcctgccc gccgaaccac cagctgtctc
     9661 gaccactgaa tgaaaggacc atctccatgg cagacattcc ctacgacacc gtgctggagc
     9721 gcgttcgcga gcgctacgtc gacgagcctg agatcatcca gcggctgcgc gaggagaccg
     9781 ccgaactccc caacgcgggc atgttgctcg gccccgagga cggcgcactg ctggcggtaa
     9841 tggcccggac gctcaacccc gaacgcacgc tcgacatcgg cgtcttcacc gggttctccg
     9901 cactcaccgt cgctctcaac gcgccccacg cccgtatcac cgcccttgac gtcaacgacg
     9961 agatcacccg gcgcgcccgc cgctactgga aggaagcgcg cgtcgccgac cggattgacc
    10021 tccgcatcgc cccggcgctc gactcgctcg acgcgctgct tcacgacgga catcagggga
    10081 gcttcgactt tgccttcatc gatgccgaca agccgaacta cccccactac gtcgaccgcg
    10141 tcaccagcct cctgcgtccg ggtggcatga tgctgctcga caatatgctc tggtcggtcc
    10201 ttgaggaacc gcaggaagac gcggagctgc tcgaccagct ctcgcgccgg atggtcgccg
    10261 acgagcgtct caccaccgcc gtgctgccgc tcggcagcgg catgaccttc gcggtccgca
    10321 accgttgacc ccgccccgct cggctatcct tgtcagagca ggaataggcc gcgtaccgct
    10381 cccggagcac cattcatggg cagccgtccc ccgcgtcacc acacactccc gaagtcaaga
    10441 ccccgccagc cgcgctcggt tcccaaccac cagccagccc agaccgggct gcgcatggag
    10501 gcggtcgcca ttttcatcgg attcgccatc ttcccggtgg cactcggcgc gtcgggcgtc
    10561 gcctacctcg ctcggcagga gatcaccgac gtcgccacgg cggtgatcct caccatcgtc
    10621 gtcgggcttg ttgccgcagt gttcgcgcgc agccgacgcc agtggcgacg agacgcccgc
    10681 atgagcaatg aactcctgct cgcgatgctg atcggcacaa tcggcgtgac cttcctcatc
    10741 ggcgggatcg ccgccatgtc gtccatctgg atcgtggtcg gcgtggctgt gctggcgggg
    10801 gcaatcctcg tcggccgcac tgcaaaacca accacgcgat aaccggcatc tcatacgggc
    10861 cgaccatcct gcagaggccg gcccttcacg tctctcgcgc catgtcacgt ttcggcaagg
    10921 attcccatgt ttacccgcac cgtccccttc cccaccgacg atgatttcgc tcacgcccgt
    10981 gaggtggtaa gccgctacct cacccgcacg ccactcgtcc cctcgcgcgt ccggcgtacc
    11041 ggatggctca aggtcgagtc gttccagccg accgggtcgt tcaaggtccg tggggcgctg
    11101 gccgcgctct ccgccgcacc tgccgacagg ccgatcatca ccgcctcggc cgggaaccac
    11161 gccctcggcg tcgccttcgc cgccaaagca ctcgatcgca acgccatggt ggtggtcccc
    11221 gagacggcct cacccgccaa gatcgaggcg ttgcgcacgc tgccggtgca gatgatcacc
    11281 catggcgacg atttcgatgc ggcggaggcg tgggcgatct cccgtgccga cgccccggcg
    11341 aaggacgtca ccttcctctc gccctacaac gatccccacg tcattgccgg ccaggcgacg
    11401 atgctggacg agatcattgc ccagcatcct ggggacaggc ccctgacggt cgtcgccggc
    11461 gccggtggcg gtggactgtt gagcgggctc ggccttggcg ccagccagct gaccaccgac
    11521 gagcgtccga ttcgcgtcgt cggtgtcgag gcggaggcct cgcagggact ctcgacggcg
    11581 gtcgcggcgg gccaggtcga ggtggttgag gtcgagccga ccctggctga tgggcttgcc
    11641 gggaacatcg aggaagggtc gatcacggtc gaactggtca agcgacatgt cgcggagatc
    11701 gtgcaggtca ccgaggacga catcgccgcc gggattcgct ggctggtgac gaaccacggc
    11761 ctggttgccg agggggcggg ggcagcgccg gtcgccgcgc tgctggcgga cctgatcgac
    11821 ccggacctgc tcgacggcga cgtcgtcgcg atcctctccg gccgcaacat cgcgcttccc
    11881 atcctcgcca ccgttctcat gggcgaggac gtcatccacg aagccgagta gaaccacttc
    11941 ctagttcatc gaaatggtcg gcaacatgta gacccgcgca tctcagcgcg ggccggtcat
    12001 tagtgtcttc tgttatcaaa tccgcacaat cgctccggat gccatccgtc ccttgagcct
    12061 gtagcgccgg ccttgcagcg gcatcgcacg cgtcgcgctg ccgcccggcg ggcccgacga
    12121 caggtgccga cggcagccgc ccaggcggtt caccagcaac cacccggatt ccgtgcagac
    12181 gaaggaaaac gggacgggcc agatgcccgt cccgtttggg gcggacatgg agtctgcccg
    12241 tacggatcgc cacgccatga cgaccctgct tttcgatccg actgcatcag gccacgtcgc
    12301 ggtccagcgc gtcgtcgacc ctccagtcat ggccgggggc gagcacccga cgcgggcgca
    12361 gcgcgaagac caccgcggcc atcgccgcca gcggccaggc gacacccggc gtcacgcggg
    12421 gctcgcccgg agctggcggt cccggctccg gggtcgaatc gagcagatcg gctgcgtcgc
    12481 cgctgcccgt gttgggcggc tgtgacggac cgacgcagtt gtagatcgag accagcgaga
    12541 cctggcccgc cttcaccacg acgtcaccct tggcgttgac gctgttggag ttcgcgtaac
    12601 accagttccc gctgacctcg cgcagctgga acgtgccggg tcgcagatcc acaaaccgca
    12661 ggatgccatc cgggttcgcc gtctgctgga cgccgtcgcc gtagtcctgc atctcctcgt
    12721 tgaaccgctt cagctcgaac gtcgacagct gatcgctcag gcggcactcg ctctcgaagt
    12781 cgtagccctt cggtggatcc tcgacatcac agacatactt gcggaccaga atcacgccgg
    12841 ttgaatccgt caccggttcg gggatgttga agaggacaca gtagaggttc tccccctcgc
    12901 ccagatccgc cgtgatcgca ccgttgatcg tcttgaaggc ggttggcgag ttcggatccc
    12961 atccacagaa ctggtaactc gtcgggatgt tgtacggtcg ctcctcgtag accgtccagg
    13021 tgcccgatgg ctgatcggcg aagaccgccc gtcccacctg acccccttca ccggtcaccg
    13081 tcttgaggtc caccggaccc tcggcccgca cggtgatgtt gttggtcagc tgcgagctct
    13141 gcatacagct cgcggcgaag tccgcgaagg tcgtgccgct gaagcccgcc tggcaggtgt
    13201 acttcgtgac gtggatgtcg accggctccg gctccggcgg ggcgacgtag ttgatcacga
    13261 tcactgtcgt catccggccg ggctcgaccc ggaggagcgc tgccgagttg cccggcaggt
    13321 cggggttcgt ctcgtccaac cggtagatct tcgccggcag cgtcacctgg taccggccgt
    13381 catcgccagt gcggaacgtc cccgggccgt cgctcccgtc ctccccgacc agcgtgaact
    13441 cggcgtcacc gcgctcgcac cccttggtct ttgccagctc ggcattgccc gccccgccac
    13501 cgaggaagtt agtgcgctcc tcgtcggcag aatcgactag gcagacgaac ttgtgcacct
    13561 gcaccgagcc cgtgttgggc ggcggctgca gctcgttttc cactgtgacg atcgtcgtct
    13621 gcgccggatg gacctcgatg ttctcgaccg gtccggactc gcggaacccg tacggcaccg
    13681 tggtctcgac cagcgtgtaa gtacccactg gcaggtcctc gaagagcagt tcgccatcgg
    13741 cattggcgca gagcgggtcg ccctgatccg gccgcagctc gaagcatgcc ccctcaatcg
    13801 ggcggccgtt cggcatcacc ttccggacca ggatgcgacc gttcttgagc cggttgacga
    13861 catcaacctc ggtcgtctca tcatcctcga tcgtcacctc ctggtccgct gccagctggt
    13921 acgccggcga gggcgccagc gtctcgcgca gcgtccatgt gcctgcgggc acattctcga
    13981 agacgattcg agcgtcgttg gtaccatcgg tgatgtcgca gactgcgccc atcaccgggg
    14041 tgtcaccgtc gtagagacgg aagcaaccac cggccagtgc ctcgttgttc ggaccgagct
    14101 tgttgaccac cagcgtgccg gcctcgggca gcgggggggt ctcctggttc tgcaccgtca
    14161 cctgcaaggt caccccggca cggatcgtga cctcgcggtt cgacgcggga tcgtatccgg
    14221 tcggcgcctg ggtctcgacc agtgtgtagg tgcctggcat gacaccgccg aagacgatgc
    14281 gcccgtcctc gttgaggtca tcgtcgtcgc agaatgggcc gtagatcgcc tcgccgcgca
    14341 actcgaagca ggcgccctcc agccgagttg atggatcttt ggcagcgacc ttgatcacgt
    14401 tcaggcggcc tggcgtcggg gtgttgacca cctcgacctc ggcatcaccg ccgggcgtga
    14461 tctcgacact gcgtgccgag ccggcttgga acccctgcgg cgcgcgcgtc tcgaccagct
    14521 catagctacc tacggggatg tccacgaacc tgatggtgcc gtcgtcggcg ccatccgcac
    14581 cgtcacacgc ttgcgccaca gtctcgtcgt cggtctgcag tcggaaacac gcacctgaga
    14641 gcgcttcgcc cgccgggttc acctttgcga ccgtcaacgc gcccgtcgtg acctcggcct
    14701 gaacctcgaa acggaccgtc ggtatctggc cgccggtcac ggtgacggtc ttcgactcgg
    14761 cgccggtgta accctccggg ggagagctca tggtcacgac atactcgccc ggcaagagat
    14821 tggtgatcag cacggtgccg acatcgggcg attcgtcacc gtcgccgcca tcacagactt
    14881 caatcggatc atcggtgccg atggtatagc aggcgcccgg caccggctcg ccggtttcgg
    14941 catcgatcgt ccggatctcg agcgagccga ggatgtacag cccgggaacg acgatctcgg
    15001 cctcaatcgt ttcgcccggc agaatctcga tgatgatcgg ctcgcgatcc tcctcaactt
    15061 gcagcaccga cgcaccggcg ggcgcgttca cgtccgtgaa gacgatctcg tacaccccgg
    15121 ccgggatgcc ccgcagcagg atcacgccct cggtggggtc gttgtcccgc tcgccgttgt
    15181 cgcaaaacgg cccgagctcc tcgcccgtat cctggttgac gagcccgatg caggcgccgg
    15241 ccaccgccag tccgtcctca ctcaccagcg ccaactgtgc gctgccggtc tgcggctccg
    15301 gcgtcggggt cgcagttggg gtcagcgtcg gagtgggttc aggcgtcggc gtcaccgtcg
    15361 cagtcggcgt cgacgtcggt tctggcgttg gcgtcaacgt agcagtcgcc gtcggagtca
    15421 acgtcgcggt cggagttggc tccggcgtgg cggtcgacgt tggctccgct gtgggagttg
    15481 aagtcggtgt ctgcgtaggc gtggacgtgg cagttgccgt cgcagtcggt gttgccgtcg
    15541 acgtcgcggt cggtgtcggc ggcacctccc cgacggtcac cgtagtctgg ccgtcagccg
    15601 ccaccgtgat cgactggggc tgcacagctt ccacccccga gatcacgctg agctcgtact
    15661 gccccggctc gatgttgcgg aacccgatca ggcccggctc ggggttctcg tcaccctcgg
    15721 cgttgtcgca ggcggtcggc gggttggcaa tgccattgcc gccgccggtg tcgagctgga
    15781 cgcagatctc ctcgccaccc agcgcggtca catcgatcgt taccgtaccg gctggctgct
    15841 ggccagcggt gcgctgggtc tcgccggtcg cttcctcggt cggcgtctcg tcgccctcgc
    15901 ccggattgac gaccagctcc gggttgtcgc ccggctcgat cgtgatctca cgtggatcga
    15961 tcgggttggt tccctgcggc accgtcgtct gtgagagcgt gtaggtgcca ctcggcacac
    16021 cgaagaagcc cgtccgaccg ttgccgggga agggatcatc agcgtcgcag ctcacgatcg
    16081 gagcctgccc ctccatggcg atctggaagc aggcaccacc gatcggctcg ccgtccgcgg
    16141 tacggagttc gacgatcagg ttggctggcg ggccagcttc ggtcgtggtt tcctcagtgg
    16201 ccgtcgcggt cgaggtgggc tcgacggtgg cggttggctc gatggtcgcc gtctgcgtag
    16261 ccgtgaccgg cagcgcggaa acattcgcgg tcacgacaac cgtctcgtca ccgccggctt
    16321 cgaccgtgac ctcccgcgga tcgacactga cgccctccgg cgccgagctc atctggagcg
    16381 tgtaggtgcc agccgggacg tcgaagaacc cggtgttgcc gttgttcgcg aactcggcag
    16441 actcccagtc ggaggtgtca cagctctcgg cgacgaccgt gccgtcgtcg agcaactgcc
    16501 agcaggcgcc gccaattggc tgctgcgtgc cgtcgaccag ggtcaccgtc aggttgcccg
    16561 ggccgacctc tggggtcggc gtttcggtcg cctccacggt cgcagtctgg gtcggctcgg
    16621 aaagctcgac agccgtggtc ggctccggcg tttcgacgac ctcggtcggc tcggcgactt
    16681 ggacgcggat ggtctcggca tcgtcgctgc ccggcgcgat ctccacctgc cgatcctcga
    16741 ccggtgtgac tccctccggc gcggcgctca tcgtgagcgt gtacgttcct ggctggatat
    16801 cgaagaatcc cgtgttgccg ttgtcggcga actcaggcga ctccgccgct tcggtatcgc
    16861 agctctcggc caccacttcg ccgtcgtgga ccagctgcca gcaggcgccg ccaacgggat
    16921 cgccgtcctg gtcaagcagt gtgaccgtga agttggccgg ctgcggagcc tccgtcgcgg
    16981 tcggctccgg tgtctcggta ggctgtgtcg tagcagtcgg ttcaggcgtc tcggtcgccg
    17041 cgagttgcgc agtgacgttc acctcgaccg tctgcccggc gatgacctcg acccgccggt
    17101 cgtctgcggt cgcaccattt ggcgcatcgc ttagccgcag ggtatacgta ccggccggca
    17161 caccgaagaa gcccgtcgtg ccgttattgg cgaactcggc ggggtcgtca ggctgacggt
    17221 cgcaccgctc ggcgacaacg ttttcgttct gaagcagctg gaaacaggcg cctgggaccg
    17281 gcgcgccgct ctgatcggac aggtagacca ggaggtcacc gggagcggcc tgctcaggct
    17341 cggtgggaac ggcggtctcc tcgccttccc cctcgatgat ttgtccgtcc tcggcagccg
    17401 gctcctctgt ggccagttcc tctgccgtcg tttcctgggt gacctcgggc tggagagtgg
    17461 tgaagagatc acccggctga agctgaatct gctggtcggc gaccggctcg gcgccggccg
    17521 gaggggtgac ctcgaccacg gagacggtgc cactcggaac gccttccggg aaggtcatcg
    17581 tggtgtagcc gtcggcgcca tcgtcggcgt cacaggcttg gttgaggagc gtgttgccgc
    17641 tgcgcagctg atagcagcca cccggcagcg gattgccatt cgggtcgaga cgctcgatcg
    17701 tgagcgacgc gccggcgagc ggctgaacgt tgaccagttg gccatccgga gagaaggtca
    17761 gccggttcgg gccttggttc aggttggcct gctgtggtgc accgagcggc tcaccaccgg
    17821 tgaagacctg caccgttgcc gggtcactct gctggacggt gaccaacgcg gtgagttgcc
    17881 cgccggcggg ctccagcgcc accgccgtgc cattgacgtt gacggagtag agctcgctga
    17941 ccgatgcagc ctcgacctca ttggtatgtg tgttcaggcg gaagtagagg ccggcatcgc
    18001 tctcgccgac ggtaacctgt tgcggactgc ccccgagccc gccctgccca taggtgtaga
    18061 ccgtgccgtc cgccgcgatc acctggatct gccagctgta ctgccctggt ggcacggcta
    18121 cgacgccggt ccaaatcccc tgatgctcgg tgagttgggt gggctggcag gagagatcga
    18181 agtctgcaca cccgagctgc gactggaagt cgccgaccgc cacaacctgg gtgagggcgg
    18241 gaggcacaac gtcgctgccg gtctgcagcc gcgtcagcgc cgatggatca ccattcggcg
    18301 cggctatcgc ggtcagaggc aggaacagcc cggtcatcag ggcgatcgcg ccgatgacgt
    18361 gcagccacca gccggcgccg gtgatgcgat cgcggcgagc cgacgttcga gccgagcgag
    18421 agcgagctgc gagcagatcc atggtattcc tccgcgtccc cggggccgcc aagaccggct
    18481 gggcatcgag ttggtcacgt acgtgagcgc ctggtggcga gtgccgccgg ttcgggagcc
    18541 ccggagatgg gactgggcag gagacgatgt gtctcccaac ggtagcacgc ggccggcgct
    18601 cgtcaactga attgcggacc gggcctcccg accaccgcaa cacggaaatc cgggaggcgc
    18661 cgagccgact cgagatcgcg cgaccggttg ggatgaggga gaagggtgga ttgcccgcgc
    18721 gtctctatgc ctccagacgc actgaggtcg gaaatggttt cgcgggggtc gggaccgacg
    18781 cgcgggccgg acgatcacct gccgcaggaa gcaggagggc gccccagtcc cccagttgtc
    18841 gtcggcacaa cacgtcgaac ccggacttga ggcgagaccg cggcaggtcc gctcggcggc
    18901 acccgggcaa cggacgtggc tctggtcgga acaggaagcg ccgggcaagc gtcctgacgg
    18961 tacagactga tcgtcgaacc gctaggcatc cacaagagcg gcgacgatgt cggtgagctc
    19021 atcgggatcg ccggtcggcg gggcgaggaa gacatcggtg atgccaatct cccggaactg
    19081 ccgcagccgc tcccaaacct cccccatcgg cccggcaacc acgagctcgc gcatcaggtc
    19141 gtccgggatc gcccgccgcg ccgcggccat gtcccccgcc tgcttcagct ccgggaagtt
    19201 gccgccgcgc tcgagcacct ccatcgtccg catcgccgtg cgaacctccg cgatgatggc
    19261 agcggtgtcg aatcccggcg actccagcag ccgctccatg cccggcaatg cgtgaatcag
    19321 cgccactgtc gccttgcgcc gctgccagac cgcttccggg tcatcggtga tcgtcaccgc
    19381 cgcacgcgca aaaaatcgca gtgcctccgc atcgcggcct tgctcggcag cgtcgcggcg
    19441 cacgtcgcgg atcgccgctt ccatgaaggt catcgacgag aggtcgttga agatcacccc
    19501 gtccgcatac cgggcggcca gcgacatcgc cttcggaccg acggcggcga ggtacatcgg
    19561 caggtgctcc tgcaccgggc cggtgacccg ctcccaacgc gtgacgcgga acgcgctcgc
    19621 atctgcggcg gcttcggcag tccacggtgg tgtgagccat tgccgcagca gctgaaacga
    19681 ctcctccagc gcggtcaccg gccagccgac ctccatgccg agcgcccgct cgtaccactc
    19741 ggtctgccca cgcccgaagc cgagaaacgc gcggccgccg gcaagggcat cgagggtgga
    19801 catcgacgcg gcaatcaggg atgggtggcg gaagtacggg ttgagcacgc cggggccgag
    19861 gcgaatgcgt acggtctcgc gagcgatggc gccgagcatt gcaatcgcat cccagccatc
    19921 cggatcctcg ctcaaccaga aggaatcaac tccggccgcg tcggccgctt tcgccagccg
    19981 gacaacccct tcaatcgccg cgtccgggcg ctcgcccagc ccctgcgcca ccgcgacctc
    20041 atagtgggat cgatcgaccg cgaaccgtaa tcgctcgccg ggcatcaggc ttcgcgcagg
    20101 tagagcacga caccgccctg ccgctcgtca ccggtgccca tgacgacgcc ggtgaccccg
    20161 ttcggtagtt cacgctcgga gccgcgctcg gcattctcgg cctcatcctt ctcgatcagc
    20221 cagccgtaca tgccgtgctt gccgcagtac cagagctgac cctgcccctc gacgggtcgc
    20281 atgcgatggc ggtcccgcgg gtgccggcgt ccgacttcac agggtgggtt gggtcctgat
    20341 gtggccatgg tgaatgcagc tgtcctcttc gaaattcggc aattcctcaa tactcgacgt
    20401 atggtacgac gccgcccaga cagagcgccg ctgactgctc cggtaaactg tgtcgcaacg
    20461 acacctgtgc cgcaccatct gtccggaggc caccatgcgg atcgccgttc tcctgctcgc
    20521 gctcaccctg ctcccggccg tgcccgccgc cgcccaggag ggaacggtca ccccggccgc
    20581 cggcccgtca ctcgccgacg tcgcacccct ggatatcctc caagcgctgg aaggggccga
    20641 cgcgcccgag accctgcccg gcaacaccga cgaggagatc gtgctgcaga cgtgggaaga
    20701 gcactacggc gagcggctga acaccacgct gggggcgtgg gtgatgaccg gctcgacgca
    20761 gctgccgatg gcctcggtac tggtcttccc gtcagccgag aacgccgtgg cgggactcgg
    20821 cgagttccgc agggactcct cggcgaccac ggcgggcacc ctcgaggcgt ggacgatcgc
    20881 cgaccggggc aagtggatct gcgttgcggt cgatggtccg gtgatgctga tcggtcaggc
    20941 ggagcccgag gtcggcgagg cggaggaaac cgttcgtgaa cgctcctgcg aggtcactgc
    21001 tgcgacccac gaatggatgc tggagacggt cggactcaca ggcggagatg caacgccggc
    21061 cgcggacggc tgaggcagta ccgcacgcca gcagaactcc ggctcgtcgt cgcccccctc
    21121 atcctcgatc tcacaaggac cgcaatggac acacccctcg tgccccgagg cgtcgacctc
    21181 atggagatcg acgagcagcg tgccttcctc gacgggtccg tcgatctcgc ccgctgcgat
    21241 taccgctcct cggcggcgta gaccagcagc acgtgcggtt cgctcaagcg ctcttcgaac
    21301 gtcaggccaa cgtcggcatc gtcattctgg gcatgggcgg gcatgctgaa ggccgcaatg
    21361 gccggcgcaa tggctcccgg tactcagcgt gggccaggca tggatcagtc gcatcgtggg
    21421 ttgtccgttc attgactacg cgcccacgac acgtcggcgc ggccgttcag caacgcatct
    21481 cacagcgcgg cgtcacccgt gacagacgca tgacgcgttc cctgtccaaa ccacagccta
    21541 ctctggggtc agcccctcgc cctccactgg gcgctcaccg gcttccgtac gtcgattcca
    21601 cgcgccagtg gccaggcggc tccactcgta ctcactcatg ccgatcgccc ctgcgaatgc
    21661 ggccaactcg gcgagccgat ccgccagctg ttcccgcgtg ccgggctgct ccgtcgccgg
    21721 cggcagctcg acgtcgtccc ggaccgtctc ctccgcctcg acgcgcctac gctcggcgtc
    21781 gacgtcggcc atccgcaacc cgccccgttc ctcaagcaga tcggcgaggc gctgctgggc
    21841 ctccggcggc aaccggccgg cgcgctccca gatggtgacg ccgagccggc cctccagcat
    21901 cgcccggcgg aggtccgggt gcatctccga ggggcgcagc cgcgtctcca ggctcgcttt
    21961 cgtgagccct gtcaggcgca tgatcccctc gacgtcgtgc ccgagatcgc ccagccgctc
    22021 gatcgcctgc cactcgctga cggcattacg cgagcgcagc gtgttggcga tcacggtcat
    22081 cgctgacact gcctccggac cgacgtgttg gagcaccacc gccggaaccc tctccaaccc
    22141 gacctccact gccgctgtta cccggcggtt gccgtcgacg atctccagcg cgatcacacc
    22201 gtcctcatcg acggtctcgc ggagcagcac gggcacgagg atgccgtggt cgtgcacgct
    22261 gcgaaccatc cgggcggtcg gcttgacgtc catcggcctc aggtcggcgg tgtctaccac
    22321 ccggaagaca cgttgcatgg atgttctcca gatcattcga cgccattcgc gcttcgcgtg
    22381 tacccttgcg ccggcccaac tgacgctcct cctgtgtcat ttcgagccgg atcccggcgt
    22441 agccgggtcc gtgtcgagaa atctctttcc tgatcgaacc cgacaccatc tgcacgagag
    22501 atttctccac tccgccccct tcgggtgctc cgatcgaaat gacagggttg ggggtgcgtg
    22561 tacattcggg cccgatggct ggttggccag gctcagccac tatagcgatt gcaagtctgc
    22621 tctcggttgg cataggatcc gcgcagtggt gtataggtaa catcttcgta gccgaacgcg
    22681 gccggtccga cgaagcgaag tccgacttct gtccgcaact cctcgggcgc ggggatgccg
    22741 atcaccgtcg cgcggagccc gtagcgcagc acctcggtgg tgagcggcgc gccggtatcg
    22801 ctgtcgatca ggcagatcag gtccggcacc accgccagca ccgctccggc gtcgtcccgc
    22861 gcgatcaggt tctcgttctg gaagtcgacc cgcagcgtgc gcccggcatc attgccgaaa
    22921 ccctccatca ccacgacgcc ccgcgcgaaa ccacccgtca tgcgacgctc gacatcggtc
    22981 accttcccgg cgaagaggcg ctgcccaccg gtgacctcgc agaccgcgac gaccgggtcg
    23041 gtcttctcgc gtcgggcgct catcaccgcc tcaccgaggc ggatcgcgag cgagagcgtg
    23101 ccggcgatca cggtcttctt cagctgggag cccgtcatca ccgggaaggc gtacccggcc
    23161 gagccaccca tctggatcgt caccgcacga gcgtagcgct caagggtggt ggcgtcgtcg
    23221 atccggttga agatcgcgat atgcccaagg tggttgcaga gcgcggctgg cgtcggggtg
    23281 acgccgtaca tcgtgaaggt ctccatctgc aactcgggga aggcgcgccc catgccgtcg
    23341 ccgtcgatca ccggcaaccc gacctgcgcg gcgacgacca tcggaaccgt ggagttgctg
    23401 cccccgatct cgctcggcac caggtgcgtc acccgcttgc cgatgtgcgc ctccagcgcg
    23461 cgcagcgcct tcaccgactc gtccgcccgc cgcagtcgct cgatgctgac cgtcggggat
    23521 cccatcccgc cgaccgaggc gacgacggcg tcatccggca cctcgttggg atcgacgatc
    23581 gcgacctgag caccctcctc gacgaacttc cgcgcgatca gcttgccgag gtaggggttg
    23641 ccacccccgc cggtgccgag gatgccggcg ccgatctcga gggcgtcgag gtgggcaggg
    23701 attacaatcc aggtcacgtt gcctcctggg cgaatcttgc atatggtcac ttgcattgtg
    23761 cgcgagactc cctcgaccgc caacgcctac agcgagcatg attctgacac gaggtccacg
    23821 ctggacggag cggacacatc cggtcgccgc atccaaccct cattgtcatt atgaccaagg
    23881 cgtgctttgc ccgctgcgtg gagaaatctc ttgcccgccg gcgctcgtcc ccatgacgcc
    23941 agcaatttct cggcttcacc cttgctgcgc tcgaattccg ctcgcaatga caaccttgaa
    24001 ttatcggtat ctcaccagca acacccagga tggccgccca tgcccgcccc gatgaccacc
    24061 gatcccgaga tcgccgtact gctcgccgag atccgcggcg gcatcgccgc gacgctcggc
    24121 aacgatctcg tcgggctcta cctcttcggc tcgctcgtct ccggtgactt cacaccaggc
    24181 gtgagcgaca tcgatctgct cgccgtctcg cagcgattca tgaccgaccc ggacatcgac
    24241 cggctgcgcg atatgcatcg ggaattcgtt gaacggcacc ctgagtggga agaccgcatt
    24301 gaggttgcct accagtcgaa gcacgggttg cgaacatggc agacggagcg gtcgccgatg
    24361 ggcatcatca gtcccggcga gccaatccac ctgatcgagg cgggtgccga gtggcggatc
    24421 aactggttct tcgtgctcga ccacggcgtc acgttggccg gtccgccacc ggagaccctg
    24481 atcgcgccga tcacgaggga ggtattcatc gaggcggcgc gggagctcgg ccgatcatgg
    24541 atcgatcgca tccacaccgt aacggatcag tgcggtgagt cctacgcgat cctgacggtc
    24601 tgccgggcgc tctactccca tcaggcagga gagctggtct ccaagcagca ggccgcacga
    24661 tgggtgcagg cgcggcgccc ggagtggtcg gagctcatcg agatggcgct ggagcgtcgc
    24721 gtctcgccca acgacaacgg gcccgacgat agcttcgatg acaccgtccg cttcattgag
    24781 acgatggccg ccgagctgga tggtgacaat gccccggacg cctccgcgtc aacgcgcgct
    24841 cggagacgcg ggttcgcaac acaggacgat tcacgagtga gcatcgcgaa atggaggctt
    24901 gaaccggcga tgccgggtga tttcgacatc ctgttcgata tccaccgacg agcgatgggc
    24961 ctctatgtcg aagcgatatg gggctgggat gacggcgacc agcggcggag atttcggggc
    25021 gcgctgaaac caggggtgca ggccgttgtt gtggacggcg ccatcgttgg catgcttgat
    25081 gtccgtcgcg gtgatgacga ggtgtttatc ggcaacatcg agctggcgcc ggagatgcag
    25141 ggacgtggga taggatcggc gatcatccga tcggtacttg ccgaagcgca agggcacggc
    25201 gtaccggtga ggctgcacgt gttcaagatc aacagtcgtg ctcgccgcct gtacgattcc
    25261 ctgggattca gcgtcgtggg cgaaaccgaa ctgcattacg aaatgctctg gcgtaccgcg
    25321 gagggcaccc gtgccaaccg ggttgacccg gaccgcggac agggcaccga ccggcgctac
    25381 gcaccggggg attcttcgct gcgctcagaa tgacacccgg ctcgccaggc attttccggg
    25441 ttcgctggac tgccccgtcc cctagtggat aatcgtcccg aagcaggacc gcacaccggt
    25501 gcatggagcg atgagcgtga tgttacggat tggaatcgac gtcggcggca ccaacaccga
    25561 cgctgccctc atgcagggca gcacggtgca cgccacctgc aagaccccaa caaccccgga
    25621 catcaccacg ggcatcgtct ccgcggtttc gaccatcctc gtgagcggtc gggtcgccgc
    25681 cgagaacatc tccagcgtga tgatcggcac gacccacttc accaacgccg tcatcgagcg
    25741 ccgccacctc gcgccgaccg cggtcgtccg cctcgccctg cccgctgccg agtcgatccc
    25801 gccgatgatg gactggccca ccgatatccg tgacgctgtc ggcaacaccg ccttcatggt
    25861 ccacggcggc aatgagttcg acggtcgcga gatccacccg ctcgtccctg aggagttgga
    25921 ggcggttgca tcggcgatcc gcgatgccgg catcaccgac atcgcgatct ccgccgcctt
    25981 cgcacccgtc agaccggcga tggaggaccg tgccgcggcg atcctcggtg agctgctgcc
    26041 acacgcatcg atcacgacct cccatgaggt cgggcagctc gggctgctgg agcgcgagaa
    26101 cgccgccatc ctcaatgccg cgctgcgccc actcgcccgg aagacggtcg ccgccttccg
    26161 ggaggcgatc accggcatgg gcatgactgc tcccctctat ctcacccaga acgacggaac
    26221 gctgatgacc gccgacttcg ccgagcggtt tccggtgctc accttcgcgt caggaccgtc
    26281 caactccgtg cgcggcgcgg cgttcctctc ggggcgcaag aacgccctcg tgcttgacgt
    26341 cggcggcacc accagcgacc tcggcgtgct ggtcgacggc ttcccgcgcc gttcctcgct
    26401 ggccgtcgat gtcggtgggg tgcgcaccaa cttccggatg ccggatgtct actcgttcgg
    26461 gctcggcggc ggctcgatcg tgacgtgtga gagggacgag gcgcaggtcg gaccgcgcag
    26521 tgtcggctac cgcatcaccg agaaggctcg cgtcttcggc ggggagacgc tcaccgcgac
    26581 cgacatcgca gtggcctccg gctggaccga cctcggtgac cgatcccgcg tcactgacct
    26641 tgacccctgc ctgctccagg tggcacagcg gcgcattcgt gagatggtct ccgccggggt
    26701 ggaccgcatg aagacgcagg caggggaagt cgatctgatc gtcgtcggag gcgggtcgat
    26761 cctggtgacg gaggtcaatg gcgtcggcga cctgattcga ccggcccacc atgacgccgc
    26821 gaacgccgtc ggtgcggcga tcgcgcagat cagcggcgag gtcgaccggg tctttgcgct
    26881 ggacgagatg acccgcgacg aagcggtgac ggtcgcgaag cgggaggcaa ccgagcgggc
    26941 agtcaccgcc ggggcggacc cggcgaccat cgagatcgtc gatgtcgagg acatccccct
    27001 cgcctacctg cccagcaacg ccacccggat ccgcgccaag gccgttggcg acgtacgcga
    27061 gtagcgccac caacctccct catgccggca cgcacaaccc aagcctccgg cggcgcccgc
    27121 cttccatggc tcaatagggc cagacggaca gtcgtcaggg tgtatgctgg attccgggat
    27181 cattatcgaa ctcacgtatg acaaccgccg caaccggggc caagttgatc aggggcagta
    27241 tgcctgtcag tggaaaacag atggaggata tcatttctgc atctccgtgg gccacactcg
    27301 tcgagcagct ccgccgcggc acgatgtcgc gccgggagtt tgtacgcctt gagacggcag
    27361 ccgggatctc ggttggggcg gccagccaca ccgcccgcca gacgggcgcc caggctaccg
    27421 ctcttgaagc cggccaggcg accggcgcaa cctcgagcac cggagccgcc gagccgggag
    27481 ggcgctcgat caaccgtgag gagctcgacc agatcatccg cgacaccttc gacctgagcg
    27541 agccggaggt catcggcgga caggtcatct acggggagat cagcgacatc gacacgctca
    27601 atccccacct ccacgtcgat atctactcca gctacctcac cgccaacatg ttcgagtacc
    27661 tcgccaccac
//
LOCUS       DASYWL010000152        10228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_513662, whole genome shotgun sequence.
ACCESSION   DASYWL010000152 DASYWL010000000
VERSION     DASYWL010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10228
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513662"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            283..1359
                     /locus_tag="VGR22_05400"
     CDS             283..1359
                     /locus_tag="VGR22_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV2128039.1"
                     /translation="MGDQQGDATGTGLQDRAAEVIDQEIDTLLSEVDGEAPLLHRMVG
                     YHLGLLASDGTPVHVDERRRLQGKRLRSSIALLVCTAAGGEIEDAGPLAAAIELLHNF
                     TLVHDDIQDRSPARRHRPTVWRIWGDAQAINAGDALFAASQIGLLNTASPHVSPDHLL
                     TISRAFNRMTIDIVRGQVLDLGFEGRSDVSVDAYLTMIEGKTAAIVQFAAWAGALLGG
                     ADIATARQYAAFGRALGLGFQIRDDALGVWGTVEATGKDPADDIRRRKQSFPVILLRA
                     AASRADREALDGIFANDRIAEEEVASVLSMMETYDISRQVEERVRAWHDDAVATTDTL
                     AATAEPRALDTLRHLIDRMADRSG"
     gene            1460..2053
                     /locus_tag="VGR22_05405"
     CDS             1460..2053
                     /locus_tag="VGR22_05405"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEV2128040.1"
                     /translation="MVTLTPRLRPMTLAYLRHELLLRKGHFAFRSGRHTGALLDRDRL
                     LADPEIASRMGYALAKAFFTDKIDTVATPSIWGAGLAQWIAYFLEPRAKVVHQSPQET
                     GRARIAENMHPMIEGQRILLVDNIVVSGDTIGQFADEVSELGGEIIGIGTLWDLADKT
                     IRGRAVIDLLGDIYPALLPEDCPLCAEGKHDVEHVPY"
     gene            2028..2726
                     /locus_tag="VGR22_05410"
     CDS             2028..2726
                     /locus_tag="VGR22_05410"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEV2128041.1"
                     /translation="MTSSTSRTRREPPKSGTVTRVSPQQSTAERMNVYLDEAYAFSMS
                     VRSLTEHPISVGDTLSEADVRRLLAADEPDRATNSSLNLLSHRGRSERELRQRLRRKG
                     YTSTAIDEAVRRVIDWGYLNDERFAEGWVEQRQASRPRSRRALAHELREKGVDRQIIE
                     TTLGEAEIDEVGDARRLASDKWRKERGQPPDKRRQRTAAFLARRGYGWDVAKRVLDEL
                     AGEKGGDVTGNDFP"
     gene            3078..3725
                     /locus_tag="VGR22_05415"
     CDS             3078..3725
                     /locus_tag="VGR22_05415"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2128042.1"
                     /translation="MADFRWYGHNGFRIRTKAGTVITDPPEKSTGYALTSRPSADIVT
                     ISNDNTEHKNLKSIKDEYALIDGPGEYEIHDIFVYGVRSYHDDQKGAEFGYNTIFVFE
                     VEGMRVAHLGDLGHPLTPDLTDALDNIDVLLVPAGGGTGIITPEQAAKLIETIEPNVV
                     IPMQFRTSRGDTGAAPIEELASHLGIDLPEPVEKISLRSTDFDDTMSFMVMSPAT"
     gene            3778..5427
                     /locus_tag="VGR22_05420"
     CDS             3778..5427
                     /locus_tag="VGR22_05420"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEV2128043.1"
                     /translation="MPKYIFVTGGVASSVGKGITTASLGRMIKARGASVSIMKLDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDENLSRASNVTTGQVYSTVIAKE
                     RRGDYLGGTIQVIPHITDEIKNRIRLASRQHDSDVVICEVGGTIGDIEGQAFIEAIRQ
                     MRRELGRRNYLFVHVTLLPKLGATGELKTKPTQQSVRELRSLGIQPDIIVSRADSEIP
                     EEVREKIALFCDVDRQAVIGMPTARTIYGVPLELEDAGLGAYVAEHFNLPREPQFNEW
                     TGMVERIVSDKKDIRIALVGKYVELHDAYLSVVESLLHAGLHHNVDIEIDWVNSETTA
                     AEEMEKILRHVSGVVVPGGFGPRGIEGKVRAVQLAREHNIPYLGLCYGLHMAVIEFAR
                     NVLGLEDANSTEIDPQTPHPVIDLMPGQHGVEMGGTMRLGLWPCKLVPDSKAGNAYGT
                     SHVDERHRHRFEVNNDYRDALEARGLVVSGASPDGTLAEIMEISDHPFFVGVQFHPEF
                     RSRLNRPHPLFRDFVGAAVQTLPDGAQRALPLEEREPVPAD"
     gene            complement(5632..7221)
                     /locus_tag="VGR22_05425"
     CDS             complement(5632..7221)
                     /locus_tag="VGR22_05425"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV2128044.1"
                     /translation="MPTYDEDGVQTGAIMAIREVSEKFVADDAGGQSPVELLVEARRR
                     VELLASLSTEIAARRGSEDFFDLIASRASEILQADAAMVMTPAANSTFVPRAMHNLPA
                     NLVGRQLNRFAFPSAVLSMAHRDIQYVNEDEASPAGVELLREIDALRRVDPDLARAVG
                     RQCGNAVQLHNVVVELEASRRRLQTTLAQLPQAVVITDEHEGNVVAMNREAQALWGTS
                     KNWLGRHASDISMLDERGQHYPPDRHPFLRPLRTRQTEFGVPMTTRGVNGEQIDVVGN
                     MAAIVGQNGELRGAVAVLQRREHFKAIDRAKDEFISVVAHELRNPLTSLRGNIQMVKR
                     RLDRMETEEARWEVDRVAGVIEQVDRIGDLVSSMLNVSRADLGKLTITPAPTDAVRLE
                     QGVIAQLESQLDGRKITIDAPNELPVEWDSIRVQQILINLITNAIRYAPEGDIDVAVA
                     PEGDTVAISVRVAALVCRSITDGGSSGCTTALMDGRHPTLRLGSSAVLASGCTSAHAL
                     PTPTAELCELMTPRAAGRSSP"
     gene            complement(7270..8316)
                     /locus_tag="VGR22_05430"
     CDS             complement(7270..8316)
                     /locus_tag="VGR22_05430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2128045.1"
                     /translation="MGETPDLPDVPAFDGGTRYTMAEAARIKGVSYHTVSRAVRSGKL
                     PAQRLGRMALIPAADLQGWRLMRQRAPRKYRKSDPDHENGELPVQAGIGASEGYGDRL
                     VSASERLMVAASAESIAGFGSWLAHWVAHSLNSETAVIWKIDEPRNRVTLYGTYGVDE
                     VHPPVVIESAAARMFRDLSDQEDVMLLDNRTAHRIQAKDWLGAMDGGLPACIPLRSGS
                     RSVGCAIVARRGQRSPLSTEDIAFAQRLGLYAAFAMEHVEMRQANQTSHVTMESVLQG
                     LPVQIVTVDSNGTVVYANRAFTDAWGEEYYERRHYSHLQRSYRVETLDGVEVRLQDNP
                     LTGALRGERVDRAI"
     gene            complement(8482..8979)
                     /gene="purE"
                     /locus_tag="VGR22_05435"
     CDS             complement(8482..8979)
                     /gene="purE"
                     /locus_tag="VGR22_05435"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEV2128046.1"
                     /translation="MTSEVRVGVVMGSDSDLPTMRAAIDVLDEFGVPREVRILSAHRT
                     PDEMVAYAREAADRGLQVIIAGAGGAAHLPGMIASVTPLPVIGVPVVATVLAGVDALL
                     SIAQMPGGVPVATVAIGNAKNAGLLAVRILSSADLQLREAMERYQQALGDAVRAKDAL
                     IRQEA"
     gene            complement(8979..10121)
                     /locus_tag="VGR22_05440"
     CDS             complement(8979..10121)
                     /locus_tag="VGR22_05440"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004680.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEV2128047.1"
                     /translation="MQYRVGIIGAGQLARMMAQAAIPLGIEVTLLAASADDGAARVVR
                     SVIVGSPDDPDRVRELAARVDVITFDHELVDPGLLDVLEAEGHAVHPRSPVMRLAQNK
                     RLQREAVDAAGLPGPAWAAIGSIGDLDAFAATHGWPVVLKAARGGYDGRGVWKLDARS
                     EAQSVVEGALGNGTELIVEAFQRLDGEVAVIVARTPGGECRVYPATETVQRDGICHEL
                     HIPARFGENVSCEAEMIARDVAEMVGSTGLLAIEFFVTDGRVLINELAPRPHNSGHWT
                     IEGAETSQFEQHLRAVLDWPLGDTAQVAASVVTRNLLGPDGGDPFARIPHALATDGGH
                     IHWYGKESRPGRKIGHITARASNHAEAERRATAVWQVLMHGAGEQG"
BASE COUNT         1850 a         3459 c         3165 g         1754 t
ORIGIN      
        1 gaacttggtg taattgccct tgaccttgct ctgcgcccag agcatgaaga tcagcccggg
       61 cagcatgaac acgaagtaca aaggatcgaa gtagaacatc tgctttcctg gctacctttc
      121 cgatacccgc gacgcactgg ccccgatata gttccagccg cggcgcgatc cctgaccgga
      181 atctatactt cgcactggtg gaattttact acgacggtgg gcggaatgcg gtgcgatcaa
      241 cgcgaaccaa cgcaccgcca tcgtgggcag tctgggggac gcgtgggaga ccaacaagga
      301 gacgcaacag ggacaggact gcaggatcgt gcggccgagg tcatcgacca ggagatcgat
      361 acgctgctca gtgaggtgga cggcgaggca ccgctgctgc atcgcatggt gggataccat
      421 ctaggcctgc tcgccagcga cggtactccc gtccacgttg acgagcgccg ccgcctgcag
      481 ggcaagcgcc tgcggtcgtc gatcgccttg ctcgtctgta ctgctgcagg cggtgagatc
      541 gaggatgccg ggccactggc cgccgccatc gagctgctgc acaacttcac cctcgtccat
      601 gatgacatcc aggaccggag cccagcccgc cggcaccggc caaccgtctg gcgaatctgg
      661 ggtgacgccc aggccatcaa cgccggcgac gccctcttcg ctgcctccca gatagggttg
      721 ctcaacaccg cctcgcctca cgtctccccc gaccacctgc tcaccatctc tcgggcgttc
      781 aaccgcatga ccatcgacat cgtccgtggg caagtactcg acctcggctt cgagggtcgc
      841 agcgatgtct cggtggatgc ctacctcacc atgatcgagg gcaagaccgc tgcgatcgtc
      901 cagttcgccg cctgggccgg cgcgctgctt gggggtgccg acatcgcaac cgctcggcag
      961 tatgccgcgt tcgggcgcgc gcttgggctc gggtttcaga tccgcgacga cgcgctgggc
     1021 gtttggggaa cggtcgaggc caccggcaag gaccctgccg acgacatccg ccgccgcaag
     1081 cagtcgttcc cggtgattct gttgcgtgct gccgcctcca gggcagaccg tgaggcgctc
     1141 gacggtatct ttgccaacga tcgaatcgcc gaggaggagg tggcgtccgt tctctcgatg
     1201 atggagacat acgacatctc gcggcaggtc gaggagcgag tccgagcgtg gcatgacgac
     1261 gccgtggcga cgaccgatac gctcgccgct accgccgagc cgcgcgcact cgacactctg
     1321 cgccacctca tcgaccgcat ggcagaccgt tccggttgaa tcaggcccac gtgctatgct
     1381 gaaaaccggt tcggcacgaa tgtgaaccgt tgcacgccgg gcgctggccc ggtcttgatc
     1441 tcgaccggga gtcccgccca tggtgacgct aacgcctcgc ctccgcccga tgacgctggc
     1501 ctacctgcgg cacgagctac tgctccgaaa gggacacttc gcgttccgct cggggcgaca
     1561 caccggtgcc ctgcttgacc gcgaccgcct gctggcggac ccggagattg ccagccgcat
     1621 ggggtatgcc ctcgccaagg cgtttttcac cgacaagatc gacaccgtcg ccaccccgag
     1681 catctggggc gccggcctcg cgcagtggat cgcctacttc ctcgaacctc gtgccaaggt
     1741 ggtgcatcaa tcgccgcagg aaaccggcag ggcgcgtatc gccgagaaca tgcacccgat
     1801 gatcgagggc cagcgcattc tgctggtcga caacatcgtc gtcagcggcg atacgatcgg
     1861 gcagtttgcc gacgaggtct ccgagctggg cggcgagatc atcggcatcg gcacgctctg
     1921 ggatctcgcc gacaagacga ttcgcgggcg tgccgtgatc gatctgctgg gcgacatcta
     1981 ccccgcgttg ttgccggaag actgtccgct ttgcgccgag ggcaaacatg acgtcgagca
     2041 cgtcccgtac taggcgcgag cccccgaagt cagggacggt cactcgcgtc tccccacaac
     2101 agtcgaccgc cgagcgcatg aatgtctatc tcgacgaggc ctatgcgttc agcatgagcg
     2161 tgcggtccct caccgagcac ccgatctccg tcggagatac gctctcggag gccgacgttc
     2221 gccggctgct ggcagctgac gaaccggacc gcgccacgaa cagctctctc aacctcctct
     2281 cccatcgtgg ccgctcggag cgggaactgc gccagcgcct gcggcggaag gggtacacct
     2341 ccacagcgat cgatgaagcc gttcggcgtg tgatcgactg gggctacctc aacgacgagc
     2401 gcttcgccga gggatgggtc gaacagcgac aggccagccg cccccgctcg cgccgggcac
     2461 tcgcacatga gctacgcgag aagggagttg atcggcagat catcgaaaca actctcggcg
     2521 aggcagagat cgacgaggtc ggcgatgcac ggcggctcgc gtcagacaag tggcgcaagg
     2581 agcgcggcca gccgccggac aaacgccggc agcgcaccgc agcattcctc gcacggcggg
     2641 gttacgggtg ggacgttgcg aagcgcgtcc tcgatgagct cgccggagag aaaggtggag
     2701 acgtgacggg caacgacttt ccgtagaggc ggcccgcgac ggctgtccgc ccgctcacgt
     2761 catggctgcg acgttgcaca ttccttacct caatccaaac gcccctcagc cgcttgcctg
     2821 gctcttgcac ccgtttcctg acaccctgcc cgtgtagcca cggttcccgc gccgctcggt
     2881 gaacgtacgc catgtggtac agtgaaagga ggctgcggga gaacgcacgt cgctcgacgc
     2941 catcccgata tccccctgtt gacacatcca tttggcggtc tccagcccgt gcagccgcgc
     3001 ggtcagaccg cgaccgatcc cgggcgtacc gcccgccggg tcgattcttc attgtttcag
     3061 ggaggagtcc gcacttcatg gccgattttc gatggtacgg acacaacggc tttcgtatcc
     3121 gcacgaaggc aggcacggtc atcactgacc cgccggaaaa atctaccggc tacgcattga
     3181 ccagcaggcc ctctgcagac attgtcacca tctcaaacga caacaccgaa cacaagaacc
     3241 tgaagagtat caaggacgaa tacgcgctga tcgacggccc cggtgagtat gagattcacg
     3301 acatcttcgt gtatggcgtt cgcagctatc acgacgatca gaagggcgcc gagttcggct
     3361 acaacaccat cttcgtcttt gaggtcgagg gcatgcgcgt cgcccacctc ggcgatctcg
     3421 gccacccgct cacaccggac ctcaccgatg ccctcgacaa catcgatgtc ctcctcgtcc
     3481 ccgccggcgg aggcacgggc atcatcactc cagagcaggc cgccaagctg atcgagacga
     3541 tcgagccaaa cgtcgtcatc cccatgcagt tccgaaccag ccgcggtgac accggagccg
     3601 ccccgatcga ggaactcgcc tcgcatctgg gcatcgacct gccagagccg gtcgagaaga
     3661 tctcccttcg cagcaccgac ttcgacgaca cgatgtcctt catggtgatg agtcccgcca
     3721 cctagggcca gcgggaagtg ctggcgtgcc gcagcgccgg cgatggagtt gattccaatg
     3781 ccgaaataca tcttcgtgac cggtggcgtg gcatcgtccg tgggcaaggg cattaccacc
     3841 gcctcgctgg gccgcatgat caaggcgcgt ggcgcgagcg tctcgatcat gaagctggat
     3901 ccgtacctca acgtcgatcc gggcaccatg tcgccttatc agcacggcga ggtcttcgtc
     3961 actgacgatg gtgccgaaac tgacctcgac ctcggtcact acgagcggtt cattgacgag
     4021 aatctctctc gcgcgagcaa cgtgaccact ggacaggtct actccacggt catcgccaag
     4081 gagcgccgcg gcgactacct gggtggcacc atccaggtga tcccgcatat tactgacgaa
     4141 atcaagaacc gtatccggct cgcctcgcgt cagcacgact ccgatgttgt gatctgtgag
     4201 gttggcggga cgatcggcga catcgaggga caagcgttca tcgaggccat ccggcaaatg
     4261 cgtcgtgagc tcggccgccg caactacctc ttcgtgcacg tcaccctgtt gccgaagctc
     4321 ggcgcaacgg gagagctgaa aaccaagcca acccagcaga gtgtccgcga gctgcgctcg
     4381 ctcggtatcc agccggacat catcgtctca cgcgccgaca gcgagatccc cgaggaggtc
     4441 cgggaaaaga tcgcgctctt ctgcgacgtc gaccgccagg cggtaatcgg catgccgacc
     4501 gcccgcacca tctacggggt cccactggaa ctggaggatg ccgggctcgg cgcctatgtc
     4561 gccgagcatt tcaaccttcc gcgcgagccg cagttcaacg aatggaccgg catggtcgag
     4621 cggatcgtca gcgacaagaa ggacatccgt attgccctcg tcggcaagta cgtggagctg
     4681 cacgatgcct acctgagcgt tgtcgaatcg ctgctgcacg ccggccttca ccacaacgtg
     4741 gacatcgaga tcgactgggt caactcggag acgaccgccg ccgaggagat ggaaaagatc
     4801 ctacgccacg tctcgggtgt ggtggtgccc ggagggttcg ggccgcgcgg catcgagggt
     4861 aaggtcaggg ccgtgcagct cgctcgcgag cacaacatcc cctacctcgg gctctgctac
     4921 gggctgcaca tggcggtgat cgagttcgcg cgcaacgtgc tcggactcga agacgccaac
     4981 tcaacggaga ttgacccgca aaccccgcac cccgtgatcg acttgatgcc gggtcagcac
     5041 ggagtggaga tgggcggcac gatgcggctc ggcctctggc catgcaagct cgtgcccgac
     5101 tccaaggctg ggaacgccta cggcaccagt cacgtcgacg agcggcaccg ccaccggttc
     5161 gaggtcaaca acgactatcg cgatgcgctg gaggcgaggg ggctggtggt cagcggcgcg
     5221 tcgccggacg gcacgctggc cgagattatg gagatcagcg accacccgtt cttcgttggt
     5281 gttcagttcc acccggagtt ccggagccgt ctgaaccggc cgcacccact cttccgcgac
     5341 ttcgtcggcg cggcggtcca gaccctgccg gacggcgcgc agcgggccct gccgctggag
     5401 gagcgtgagc ctgtcccggc ggattagaac agcttgcagt gatgctgaca acgtaggcgc
     5461 agaccttccg ctgcagcctc gggtcttgtg ccgagattcg acgcgtggct cttgtgtgcg
     5521 gctaggccat ggctctatgt gatgaaacgg gggcggctgt gtagccgcct catcacgcct
     5581 actgctgctc acgggacgat caggactgcg gtgtggcgac ccgcggcaat gtcaaggtga
     5641 agatcgcccc gccgccctcg gcgtcatcaa ctcgcaaagc tccgccgtgg gcgtcggcaa
     5701 ggcgtgcgct gatgtacagc ccgatgccaa gaccgccgag ctgcccagcc ggagcgtcgg
     5761 gtgtctccca tccatcaagg cggtagtaca gccggaagag ccgccgtcgg tgatggagcg
     5821 gcacaccagg gccgcgactc gtaccgagat tgccacggtg tcgccttctg gcgccaccgc
     5881 tacatcgata tcgccctctg gcgcatatcg gattgcgttg gttatgaggt tgatcaggat
     5941 ctgctggacc cgaatgctgt cccactccac tggaagctcg ttcggggcat cgatggtgat
     6001 cttccggccg tccagctgcg actcgagctg ggcgatcacg ccctgctcta ggcgcacggc
     6061 gtcggttggt gcaggagtga tcgtcagctt gccgagatcg gcacgcgaga cgttgagcat
     6121 gctgctgacc agatccccga tgcggtcgac ctgctcgatc acaccggcca cgcggtcgac
     6181 ctcccatcgc gcctcctccg tctccatccg gtcaagccgg cgcttcacca tctggatgtt
     6241 gccgcgaagc gaggtgagcg ggttgcgcaa ttcgtgcgcg accacggaga tgaattcatc
     6301 cttcgcccgg tcgatcgcct tgaaatgctc gcgccgctgc aacactgcca cggccccgcg
     6361 cagttcgccg ttctgcccaa caatcgccgc catgttgccg acgacatcga tctgctcgcc
     6421 gttgacgccg cgggtggtca tagggacgcc aaactcggtc tgtcgtgtgc gaagcgggcg
     6481 caggaaagga tggcggtccg gcggatagtg ctgtccccgc tcgtccagca tagagatgtc
     6541 gcttgcgtgc cggcccagcc agttcttgga cgtcccccac agcgcctgcg cctcacggtt
     6601 catcgccacg acgttgccct catgctcgtc ggtgatcacg acggcctgcg gcaactgagc
     6661 gagcgttgtc tgcaaccgtc gccgagacgc ttccagctcg accaccacgt tatggagctg
     6721 cactgcgttg ccgcactgtc ggcccaccgc tcgggcgaga tcaggatcaa cccgacggag
     6781 cgcatcgatc tctcgcaaca gctccacgcc agcaggacta gcctcatcct cgttcacgta
     6841 ctggatatca cggtgcgcca ttgacaggac tgcggacggg aacgcgaagc gattcagctg
     6901 gcgacccacc agattggccg gaaggttgtg catcgcgcgg ggcacgaagg tgctgttggc
     6961 ggccggtgtc atcaccatcg cggcgtcggc ctgaaggatc tcactggcac ggctggcgat
     7021 caggtcaaaa aagtcctccg agccgcggcg cgccgcaatc tcggtcgaga ggctcgccag
     7081 cagctcgacg cgccgacggg cctcgacgag cagctcgacc ggcgattgac cgccagcgtc
     7141 atcagcgacg aatttctctg acacctctcg gatcgccata atggcgcccg tctgcacacc
     7201 atcctcgtcg taggttggca tggcgttgac gtcgaagacc tgcgtctcac cgggcgcctc
     7261 ggggacgagt tatatggcgc ggtccacccg ttcgccccga agcgccccag tcagaggatt
     7321 gtcttgcagg cgaacctcga cgccatcgag cgtctcaacc cgataggagc gctgaagatg
     7381 cgagtaatga cggcgctcgt agtactcctc gccccaggca tcagtgaagg cacggttggc
     7441 gtagacgacc gtgccgttgc tatccaccgt gacgatctgg acgggcaggc cctggagcac
     7501 cgactccatg gtgacgtggc tggtctggtt tgcctgccgc atctcgacgt gctccatggc
     7561 aaatgcggca tagagcccca gtcgctgggc gaaggcaata tcctccgtcg agagcggcga
     7621 gcgctgcccc ctccgcgcga cgatggcaca gccaacggat cggctgccgg accgcagggg
     7681 tatacacgcc gggagacctc catccatcgc acccagccaa tctttggcct ggatccgatg
     7741 tgccgtccgg ttatcgagaa gcatgacatc ttcctggtcg gacagatcac ggaacatgcg
     7801 agccgcagcg ctttcgatca ccactggcgg gtgtacctcg tcgacaccat acgtcccgta
     7861 caacgtgacc cggttccgtg gctcatcgat cttccagata acggccgtct ccgagttgag
     7921 gctgtgcgcc acccaatgcg cgagccagct cccgaaacca gctatcgatt cggccgacgc
     7981 cgcaaccatg agtcgctcgc tggcgctcac caagcggtcg ccatagcctt cggaggcgcc
     8041 aatccccgct tgcacgggga gctcgccgtt ttcgtggtca ggatcggatt ttcggtactt
     8101 gcgcggcgcg cgctggcgca ttaggcgcca tccctgcagg tcggctgcgg ggatcagtgc
     8161 catgcgcccc aggcgctgcg cgggcagctt gccgctgcga acggcgcgcg acacggtgtg
     8221 gtatgacaca cccttgatgc gagcggcctc ggccatcgtg tatcgagtgc caccgtcgaa
     8281 tgctggtacg tcaggtaggt cgggggtctc tcccatcgcg cgtccctgcc tcgctgcagt
     8341 tgtcttccgg atgttgctcg tgcatccgga cggagcgttc agcccccctt gagcgtcact
     8401 ccatccacat gttcgggata cgcgatctgc aaggatatgt ggatacatcg gacgagccga
     8461 cctgccttga acggagcggc atcaggcttc ctgccggatg agagcgtcct tggcgcggac
     8521 agcgtctccc agcgcctgct ggtaccgctc cattgcctca cgaagctgaa gatcagccga
     8581 ggagagaatg cgcacggcga gcagtcccgc gtttttcgcg ttgccgatgg cgacggtggc
     8641 caccgggacg ccgcccggca tctgggcgat cgacagcaac gcatcgaccc cggcaagcac
     8701 ggtcgcgacc accggcacac cgatcactgg caagggcgtc accgaggcga tcatgccagg
     8761 gagatgagcg gccccccccg caccggcgat gatcacctgc agcccacggt cggcagcctc
     8821 gcgcgcgtaa gcgaccatct cgtccggcgt ccggtgggcg ctcagaatgc ggacctcccg
     8881 aggcacaccg aactcgtcga ggacatcgat cgccgcccgc atcgtcggga ggtcggaatc
     8941 gctgcccatc acgacgccga ctcgaacctc actcgtcatc acccctgctc cccggctcca
     9001 tgcatcagga cctgccagac ggctgttgcc cggcgctcgg cctctgcatg attgctcgct
     9061 cgtgcagtga tatgcccgat cttgcgcccc gggcgactct ccttgccgta ccagtggata
     9121 tgtccgccat ctgtggccag cgcgtggggg atgcgtgcga agggatcgcc accgtccggc
     9181 ccgagcaggt tccgggtaac gaccgacgcg gcaacctgcg ccgtgtcgcc gagcggccag
     9241 tcaagcacag cccgcaggtg ctgctcgaat tgcgaggtct ccgccccctc aatggtccag
     9301 tggccagagt tatgcgggcg cggggcaagc tcgttgatca acacgcgccc atccgtgacg
     9361 aaaaactcga tcgcaaggag cccggtggag cccaccatct cggcgacgtc ccgggcgatc
     9421 atctcggcct cgcaagaaac gttctctccg aaacgggcag ggatgtgcag ctcgtggcag
     9481 atgccgtccc gctggaccgt ctctgtcgcg ggatacactc gacactcgcc gccgggcgtg
     9541 cgcgcgacga tcaccgcgac ctcgccatcg agccgctgga acgcctcgac gatcagctcg
     9601 gtcccattgc ccagcgcgcc ctcgacaacc gactgggcct cactccgggc atccagtttc
     9661 cagacaccgc ggccgtcata cccaccacga gcagccttga gcactacagg ccagccgtgg
     9721 gtcgcagcga aggcgtcgag atcgccgatg ctcccgatcg ccgcccacgc cgggcccggc
     9781 aggcccgccg cgtcgaccgc ctcgcgctgc agccgcttgt tctgcgcgag acgcatgacc
     9841 ggcgaccgcg gatgcacggc gtgcccctcc gcttcgagca cgtcgagcag gcccggatcg
     9901 acgagctcgt ggtcgaacgt gatcacgtca acgcgagcgg cgagctcccg aacgcgatca
     9961 ggatcgtccg gcgatccgac gatcacgctt cgcacgaccc tcgctgctcc gtcgtcggcg
    10021 gacgccgcga gcagcgtcac ctcgatgccg agcggaatcg ccgcctgcgc catcatccgc
    10081 gcaagctgac cggcgccgat gatgccgacc cgatactgca cctgctggtc cttccgtttc
    10141 gccggtgccg gaaactactc gcgctccggg agggcggtcg gatcgttcac tttctcccgg
    10201 aaacgctgac ggaagcgctc ctccgcat
//
LOCUS       DASYWL010000153         5850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_663817, whole genome shotgun sequence.
ACCESSION   DASYWL010000153 DASYWL010000000
VERSION     DASYWL010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5850
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663817"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VGR22_05445"
     CDS             complement(<1..232)
                     /locus_tag="VGR22_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128048.1"
                     /translation="MDSTPSTGTPIPDITDILGDDNALGSDDEGFLCQDINDDGTYEI
                     GIDEDGDGVLDEDEVLGEDLNEDQTLTQDELDP"
     gene            complement(845..1339)
                     /locus_tag="VGR22_05450"
     CDS             complement(845..1339)
                     /locus_tag="VGR22_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEV2128049.1"
                     /translation="MPDRAEAADHARELERRFFAEAGRRRSIRVAVRALIILDGHILL
                     QRLAYTGSVWFFPGGELEFGESLGAGLRRELNEELSLDVDRVIYRLAANNRFEREGVR
                     FHLLEHYFEVTPVSTDIESLESSVLVEWHALNSLHMLDVRPVDVRDILDLPDWRDVRS
                     LESE"
     gene            complement(1323..3788)
                     /gene="lon"
                     /locus_tag="VGR22_05455"
     CDS             complement(1323..3788)
                     /gene="lon"
                     /locus_tag="VGR22_05455"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEV2128050.1"
                     /translation="MRQHPALSLDDPDATFAEDGRLPLLALKNVVIFPRNVVTLLIAR
                     NRSIHAVEEALSGNQELVVTTPHDPIIDDPEPEDMYDIGTRVSILSAERQQGGNFQVV
                     LEGIGRVRLTAFDHSRPFYTVVPIDLKEPEFDPHEARIMIEHIQELAQRYAEAKDTLS
                     TEVLEMIQQATHPGHLADMLATQVLDDVPRRQRLLETTDPLVRLERLGARLTDQLEVV
                     ELERRIRDRVRDNMDKSQREYYLRQQLEVIHDELGGGEGNEIEQLRATIGERQMPALV
                     REKLQRELTRLERMPGVSAEATVARTYIDTVLALPWTECTEDRIDLDEADRVLSADHY
                     GLDTVKERISEFLAVRKLTASAGTVTSAQILCLVGPPGVGKTSLGHSVAEAMSREFIR
                     VSLGGVRDEAEIRGHRRTYIGAMPGRIISAMKTAGTINPVILLDEIDKLASDYRGDPT
                     AAMLEVLDPEQNSTFTDHYLDTTYDLSQVLFITTANSAQAIPGPLRDRMEMIQVPGYT
                     EDEKIEIARRHLLRRQLQASGLTEQSVAISDDVWRHIIRGYTREAGVRGLDRAVGQLL
                     RKVATLVVRGKTDPSGTVQIDEERLEEFLGPRKFGFEQDLGGSQVGLAIGLGTTEIGG
                     EIIPVEVATMPGHGGLTITGRAGDVMQESARAALSYARSRSDELRIDRDFQDKLDLHI
                     HLPEGATPKDGPSAGITMATAVISALTRRPVRADTAMTGEISLLGRVLPIGGIRDKAL
                     AAHRHGLRRLIVPIDNSRDLHDIPANVRDEIDIVLVNTMDQVIQEALILDDQQIEGMQ
                     EMTDRRYASVPRIHPDIIDAGSR"
     gene            complement(3900..4700)
                     /locus_tag="VGR22_05460"
     CDS             complement(3900..4700)
                     /locus_tag="VGR22_05460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEV2128051.1"
                     /translation="MPSPRSNASRTGGDRRRTMQRRRDANDRQLLSWTEEDRRRAAAF
                     THADQWRVLRILGEFVEGFDALAELGPAITIFGSARADEDDPMYEAARTAAGLLAKTG
                     FTIITGGGPGIMEAANRGAAEADGESVGIGIDLPFEQGTNDYVTLPLEFNYFFVRKTM
                     LAKYASGFVFFPGGYGTMDELFEALTLIQTGKMGPMPIVLFSSEYWAGLRDWVIDTMQ
                     KSGNISPGDEALMQITDDPHHVVEIMLDHMRERHAEDEKNRPESEFRR"
     gene            4868..5353
                     /locus_tag="VGR22_05465"
     CDS             4868..5353
                     /locus_tag="VGR22_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128052.1"
                     /translation="MALLCLVGVLLLVGCAGGDGRETAQPTTPAEAAIPTAVQPEIPL
                     EVGEFVWASNLDPETGAPAGSLTSLPNDAEQVIAVLQVPSLPAGTTVQAQWTIDGDPI
                     PGLEPEPVVAEESLEEAWLSWRLSWNAEEPWPVGTLGIRIEIDGEEVASADIPIVRAS
                     L"
     gene            5473..>5850
                     /locus_tag="VGR22_05470"
     CDS             5473..>5850
                     /locus_tag="VGR22_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128053.1"
                     /translation="MEPFEPGSNNPVANLRTDGPSVPWTPLEIHAERRCTSAATAVPF
                     VVQAGPSVLMIEQSHDGWVLAELRFDGEECLFRDIRLSSYAWPREAFGVLLSRLASSD
                     LDAETVDQLSTDFAEWVGIGFARI"
BASE COUNT          949 a         1799 c         1995 g         1107 t
ORIGIN      
        1 caggatccag ctcatcttgc gtaagcgttt ggtcctcgtt caggtcctcg cctagcacct
       61 cgtcctcgtc aaggacgccg tcgccgtcct cgtcgatgcc gatctcgtag gtgccgtcgt
      121 cgttgatgtc ctgacacagg aagccttcat cgtccgatcc gagcgcgttg tcatcgccca
      181 agatatcggt gatgtcggga atcggtgtgc ccgtacttgg tgtgctgtcc accgagatct
      241 cggctgtgtc ggtttcctcg aggatctcga cgacaggcgt gccgattcca ggggggcttc
      301 accaatctct gccgcctggg tattgccgag gacatccgtg atatttgtga caggcgtgcc
      361 ggctgtgggg gtggcggctt catcccggat gatgccgcaa atctcctggt agcacgcgaa
      421 ctcgcgttcg gccgttgtcc gctgagcgct ctccgccgcc gcttgcgtgc ctccgagcag
      481 gaacagcagg agcagggccc ctagcactcg cacagaactt cccaatcgca tcgtgacctc
      541 ctgttgcagt agcccatgcg ttggacggcg ggcgaggaca gcggcgccag gtgacgcagc
      601 gctaaaacct cccaagggct atgccagttg caaggccatg tcgatcaatt gcggtcgcga
      661 ggtccatatg ggctcgtctg ctgacaagcg ttatgagggt tcgacttgca ctggggggct
      721 atgcaactgc tcaatgtaga ggctcgtctg caagaactcg ctcccgtgcc tggccgacag
      781 ccggtaccga taccaaggcg cgccgcaacc cccggccctc gcgggtcgaa catggcgagt
      841 gcgcttactc gctttcgagt gaacggacat cgcgccagtc ggggaggtcg agaatgtccc
      901 gcacgtcgac cgggcggacg tcgagcatgt ggaggctatt gagcgcatgc cattccacga
      961 gcaccgatga ttccaggctc tcgatgtcgg tcgatacagg ggtcacctcg aagtagtgct
     1021 caaggaggtg gaacctgacg ccctcgcgct cgaaccggtt gttggctgcc agccggtaga
     1081 tcaccctgtc gacatcaagt gagagttcct cgttgagctc acggcggagt ccggcgccga
     1141 gggactcgcc gaattccagc tccccgccag ggaagaacca cacggagccc gtgtaggcaa
     1201 gccgctggag cagaatgtga ccgtcgagga tgatcagggc acgcaccgcg acgcggatgg
     1261 acctgcgccg gccggcttcg gcaaaaaagc ggcgttcgag ttcccgcgcg tggtccgctg
     1321 cctcagcgcg atccggcatc gatgatgtca ggatgaattc gcggcaccga cgcgtatcga
     1381 cggtcggtca tctcctgcat gccctcgatt tgctggtcgt cgaggatcaa cgcctcctgg
     1441 atcacctggt ccatggtgtt gaccagcacg atatcgattt cgtcgcggac gttcgccggg
     1501 atgtcatgca ggtcacggct gttgtcgatc gggacgatca gccgccgcag gccgtgccgg
     1561 tgggcggcca gggctttgtc acggataccg ccgatcggca gcacccgtcc gagcagggag
     1621 atctcgccgg tcatcgctgt gtcggcgcgc accggtcggc gagtcagcgc cgagatgacg
     1681 gcggtcgcca tggtgatgcc agcggaggga ccgtccttgg gtgtcgcgcc ctctggcaga
     1741 tggatgtgga ggtcgagctt gtcctggaaa tcacggtcga tacggagctc atcggatcgt
     1801 gatcgtgcgt aggatagcgc cgcacgggcg gactcctgca tcacgtcgcc ggcacggccg
     1861 gtgatcgtca ggccgccatg acccggcatg gtcgcgacct ccaccgggat gatctcgccc
     1921 ccgatctcgg tcgtaccgag gccgatggcg aggccgactt ggctgccgcc aagatcctgc
     1981 tcgaagccga acttgcgcgg cccgaggaac tcctcgaggc gctcctcgtc gatctgcact
     2041 gtcccactcg gatcggtctt gccgcggacg accagcgtcg ccaccttgcg caacagctgg
     2101 ccgacggcac ggtcgagccc gcggacgccc gcctcccgtg tgtagccacg gatgatgtgt
     2161 cgccacacgt cgtcggaaat cgccaccgac tgctccgtga ggccgctggc ctgcagctgc
     2221 cggcgcagca ggtgccggcg cgcgatctcg atcttctcgt cctcggtgta cccgggaacc
     2281 tggatcatct ccattcggtc gcggagcggt ccggggattg cctgcgcgga gttcgccgtg
     2341 gtgatgaaga gcacctgtga gaggtcatat gtcgtgtcga ggtagtggtc cgtgaacgtg
     2401 gagttctgct cagggtccag cacctccagc attgcggcgg ttggatcgcc acgatagtca
     2461 gaggcaagct tgtcgatctc atcgagcagg atcaccggat tgatcgtgcc ggcggtcttc
     2521 atcgcgctga tgatacgccc aggcatggcg ccgatgtagg tacgtcgatg tccgcggatc
     2581 tctgcctcgt cgcgaacacc gccgagcgag acacggatga actcgcggct catggcctcc
     2641 gcaacggagt ggccgaggct cgtcttgccc acacccgggg gaccaacgag acagaggatc
     2701 tgcgccgacg tgaccgtacc ggcacttgcg gtcagcttgc gaacggcaag aaactccgag
     2761 atgcgctcct tcaccgtgtc gaggccgtag tggtcggcgc tgagcacgcg gtcggcctca
     2821 tcgaggtcga tgcgatcctc ggtgcactcc gtccatggga gcgcgagaac ggtgtcgatg
     2881 taggtgcgtg cgacggtcgc ctcggccgag acgccaggca tccgctccag ccgggtcagc
     2941 tcgcgctgga gcttctcgcg caccagcgca ggcatctgcc gctcgccgat cgtcgcacgc
     3001 agctgctcga tctcgttgcc ctcaccaccg ccgagctcgt cgtggatgac ttccagctgc
     3061 tggcggaggt agtactcgcg ctgcgacttg tccatgttgt cacggacgcg atctcggatg
     3121 cgccgctcca gctcgaccac ctccagctgg tcagtgaggc gcgcgccgag ccgctccaag
     3181 cgaaccaggg ggtcggtcgt ctccagcaga cgttggcggc gcggtacatc gtcgagtacc
     3241 tgggtcgcca gcatatcggc gaggtggccg gggtgggtcg cctgctggat catctccagc
     3301 acctcggtgg agagcgtgtc cttcgcctcg gcgtagcgct gagcgagctc ctggatgtgc
     3361 tcgatcatga tccgcgcctc gtgcggatcg aactcgggct ccttcaggtc gatcgggacg
     3421 actgtgtaga aggggcggga gtggtcgaag gcggtgagcc gcacacgccc tatgccctcc
     3481 agtaccacct ggaagttgcc gccctgctgc cgctcggcgg agaggatgct gacgcgcgtg
     3541 ccaatgtcgt acatgtcctc gggttcggga tcgtcgatga tggggtcgtg cggggtcgtg
     3601 acgaccagct cctggttgcc ggagagcgcc tcctcaactg cgtgaatcga gcggttgcgc
     3661 gcgatgagca gggtgacgac gttgcgcggg aagatcacca cgttcttcag cgcaagcagc
     3721 ggaagcctgc cgtcctccgc gaaggtggca tcggggtcgt caaggctcag tgctgggtgt
     3781 tgtcgcattc gacgacatgc ctccggtgca ggtcaagccg gcccgtgggg tgtgttggac
     3841 ggggccgtcg cgggtggtgc ttcgcgggcc tcatgatacc ccacctgcga gggccagtgc
     3901 taacgcctga actcgctttc aggacggttt ttctcgtcct cggcgtgccg ctcgcgcatg
     3961 tggtcgagca tgatctccac gacgtggtgc ggatcgtcgg ttatctgcat gagcgcctcg
     4021 tcgccgggcg agatgttgcc gctcttctgc atcgtatcga tgacccaatc gcggaggcca
     4081 gcccagtact cgctgctgaa gaggacaata ggcatggggc ccatcttgcc ggtctggatc
     4141 agggtcagcg cctcgaatag ctcgtccatg gtgccgtagc caccgggaaa gaagacgaac
     4201 ccggaggcgt acttggccag catggtcttg cgcacgaaga agtagttgaa ctcgagtggc
     4261 agcgtcacat agtcgttggt gccctgctcg aatgggaggt cgataccgat tccgaccgac
     4321 tctccgtccg cctcggcagc tccgcggttc gccgcctcca tgatgcctgg tccgccaccc
     4381 gtgataatgg tgaagccggt cttggccagc aggccggcgg ccgtacgcgc cgcctcatac
     4441 atcggatcat cctcatcggc gcgggcagag ccgaagatcg tgatcgccgg tccgagctcg
     4501 gcgagcgcgt cgaacccctc gacaaactcc ccgaggatgc gcaggacccg ccactgatcg
     4561 gcgtgggtga acgcggcggc gcggcggcga tcctcttcgg tccagctcag cagctggcgg
     4621 tcgttggcgt cgcggcgtcg ctgcatcgtc cggcggcgat ctccgccggt acggctggcg
     4681 ttgctcctgg ggcttggcat ggtcaggtcc ttggttcgtc gcgccgggga acaatcgttc
     4741 cggcggggtg atcggtgcgc gtacaattcc ggatgaaatg gtaccgtgcg ggggatgggg
     4801 aagaggtatg agcggaaccg gactcggcgg ctgggctggg ggtgcgaaca gatccaggcg
     4861 acgagccatg gcattgctct gcctcgttgg cgtgctgctt ctggtagggt gcgccggcgg
     4921 agacggccga gagactgctc agcctactac ccccgctgag gctgccatcc ccactgctgt
     4981 ccagcctgag attccgctcg aggtcggcga gttcgtctgg gcatcgaacc tcgacccaga
     5041 gaccggcgca ccggccggca gcctgacctc actaccaaac gacgccgagc aggtgattgc
     5101 cgtgctgcag gtgccgtcgc tgccggccgg tacgaccgtg caggcgcagt ggacgatcga
     5161 cggcgatccg attcccgggc tcgagccgga gccggtggtg gcggaggagt cactggagga
     5221 ggcttggttg agctggcggc tctcctggaa cgcagaggag ccgtggccgg ttggtacgct
     5281 ggggatcagg atcgagatcg acggcgagga ggttgcgagt gcagatattc ccatcgtgcg
     5341 ggcaagcttg tgacacgtgc tgtggcgtgg aagtggcatt ggcaagctgc tggtgctagg
     5401 ctctacatag gacgcgatcg cggtcgcaca tgaggtgcct ggggcgatcg cgctctgtgg
     5461 caggagcatc cgatggagcc attcgaaccc ggcagcaaca acccggtggc gaatctgcga
     5521 accgacggcc cgagcgtgcc gtggacgcct ctggagatcc atgcggagcg gcgatgcacc
     5581 agcgcagcga ccgccgtgcc attcgtggtg caggcgggac cgagcgtgct catgattgag
     5641 cagtcgcacg atggctgggt gctggccgag cttcggttcg atggcgagga gtgtctcttt
     5701 cgggatatcc ggctctcgag ctacgcttgg cctcgggagg cgttcggcgt gctgctgagc
     5761 cggctggcgt cgtccgatct cgatgcggag acggtcgacc agttgagcac ggacttcgcc
     5821 gagtgggtcg ggatcggatt tgcacgaatt
//
LOCUS       DASYWL010000154         5558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_627040, whole genome shotgun sequence.
ACCESSION   DASYWL010000154 DASYWL010000000
VERSION     DASYWL010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627040"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="VGR22_05475"
     CDS             complement(<1..1036)
                     /locus_tag="VGR22_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEV2128054.1"
                     /translation="MKDQDRTEADVVGSFHGLRALVIGDALLDTFLEGSAPRISREAP
                     VPVVEQQAEHHFPGGAANAAANLSALGAEVAFVGMVGDDVTGTSLRSALQEAGVDDRW
                     LVTDPNVTTARKLRVLAHDQYLVRVDEGSLSACAPGARRALLNHVERLGPQSDLIVIS
                     DYGYSTVSEEIVEWLCKLRADHPCTLAIDSRDVRRFSRAGATLVTPNLEEARVAVAPG
                     NPSEAEPMDVARRLREKLDAENIAITMGGDGVLLADAEGHITHLPTRPIRKPDDVGAG
                     DSFIAAAALALATGARPELAARIGIDAATIVCTKPRVAVVTNRELLRRVSLSQQRAPS
                     APLSVKEIAVR"
     gene            1321..2397
                     /locus_tag="VGR22_05480"
     CDS             1321..2397
                     /locus_tag="VGR22_05480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEV2128055.1"
                     /translation="MQHGNRVPGVRRIAVLRANAIGDFIFTLPALEALRAAYPDAEIV
                     LLGKPWHAAFLADRPGPVDRVIVIPPCRGVGELEDYPNDSETLDRFFAEVAEEDFDLA
                     LQLHGGGRYSNPFACRVGARVTVGLKTPDAMPLDHWVPYVFFQNEILRYLEVVSLVGA
                     PPVTLEPRVTVTDADLAEAAPVVPHDNRPLVILHPGAGDPRRRWPPQKFAAVGDALAA
                     SGMRIGVIGTGEDERHLVDTVVGTMRADAVNLWEKLTLGGLAGLLSRSPLIVSNDSGP
                     LHLAAAVGAATVGIYWCFNLINAGALTRARHRPLLAWRLECPACGLNCLEGQCDHQDS
                     FVSDVPVEDVIDQATELLAQSSRQ"
     gene            complement(2379..2795)
                     /locus_tag="VGR22_05485"
                     /pseudo
     CDS             complement(2379..2795)
                     /locus_tag="VGR22_05485"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
     gene            complement(2919..5471)
                     /locus_tag="VGR22_05490"
     CDS             complement(2919..5471)
                     /locus_tag="VGR22_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HEV2128056.1"
                     /translation="MPTTTPSNASTFLSSGKLAHFDPGKGVEAGAAAGDFDDTGWLEI
                     TIPGDVHRTLIDAGEIPDPFWDQQEKDCAWMEEKEWWYHVPLHVPDVELGQHERWQLV
                     FHGLDTFVTIYLDGEEIGTSQNMFRDTVVDITEHVQAGREHTLALQFHPPLLAIEGRH
                     LGEWAANGRERTAMRKAQFGYGWDWGPRLPTVGTWRPIELRRVDRGMILGIHFDNVDL
                     SPDHTQAVVAVRVEAERVAGTDPLSARVTLREPASDREWTAELDLTESPAGHADGCAY
                     LTIGNPKLWWTHDLGEPFLHDLTVELIAGDDVVVTDERKVGIRSLVLDQSPDLEEKGA
                     RFFRFVLNGVPIFAKGADWIPPDSFVGAIEDERYTRWLEDARDANIQMMRVWGGGIYE
                     HDRFYDECDRVGILVWQDFMFACAAYPEDDPTFVQEVDREARYQVRRLRSHPSLAVWV
                     GNNENQWINDKTYWNVEDPPPWGALYYNHILPDAVYELDGRTPYWPGSPYGGNDHNSM
                     EDGNRHNWDVWHGQVSPRRFGEKPEQIFTPEMVSYRRYAEDMTRFCSEFGMHASPVIE
                     TLRRVVPEDELYHHSPAMDWHNKDNPKDKGDMLMAATTGVSENLEDYIDFSQIAQAEG
                     LKFGIEHYRRRKPHCSGTLVWQMNDCWPVLSWAVMDYYGFGKAGYHYLRRVYSPVLAS
                     FKETDDGVELWITNDTLERIEDEVTITLGTFGGEVVEERQLYVDLPPNSSERVDVWPS
                     DVIASNRYLRVESPGDRYPDNRHFFVDLKDLDRRPAEPKVEKRQVSDHELEVILTAPE
                     DGYCWFVHLQRPVATTRYSDNYVDIARGRSKTIVVRDASSVVDPEEVTVAWR"
BASE COUNT          933 a         1874 c         1717 g         1034 t
ORIGIN      
        1 gccgaacggc gatctccttg accgacaggg gggcagacgg cgccctctgc tgactcaggc
       61 tcacgcgccg caacagctcg cggttcgtaa ccactgcaac ccgcggcttg gtgcacacga
      121 tcgtcgcggc atcgatgccg attcgggcag caagctcggg acgagcgccg gtcgccaggg
      181 cgagcgctgc tgcggcaatg aacgaatcgc ccgcccccac atcatcaggc ttcctgatcg
      241 gacgggtcgg gagatgggtg atgtgccctt cggcgtcagc gagcagcacc ccgtctcccc
      301 ccatcgtgat agcgatattc tccgcgtcca gcttctctcg caagcgtcgc gcgacatcca
      361 tcggctctgc ctctgacgga ttccccggtg ccactgccac ccgcgcttcc tccagattcg
      421 gtgtgacgag cgttgccccg gcacgggaaa agcggcgtac gtcgcgagaa tcgatggcca
      481 acgtgcaggg atggtcggcc cgcaacttgc acagccactc gacgatctcc tcgctcaccg
      541 tcgaatatcc gtagtccgat atgacaatca ggtcggactg cggcccgagg cgctcgacat
      601 gattgagcaa ggcccgacgt gcgccaggcg cgcaggccga gaggctgcct tcgtccaccc
      661 gaacgaggta ctgatcatgg gcgagcacgc gaagtttgcg cgcggtcgtg acgttcggat
      721 cagtgacgag ccaccggtcg tcgacccccg cctcctgcaa tgccgaccgc aggctcgttc
      781 cagtcacgtc atcgccgacc attcccacga acgcgacctc ggcgccaagc gcgctcaggt
      841 tggcggctgc gttcgccgcg ccgcccggga agtgatgctc ggcctgctgc tcgaccacgg
      901 gtaccggtgc ttcccgggag attcggggcg ctgatccctc caagaaggta tcgagcagtg
      961 cgtccccaat gaccagtgcc cgcagtccgt ggaagctccc aacgacatcg gcttcagttc
     1021 gatcctgatc cttcatcgcg ctggcggttc ctctatcgct tggtggttgc agtcgcagcg
     1081 agtccgccat gctctgatgg agtgttcgaa acttgcgaac gagcgcgcct atacaacgat
     1141 ctggacgagg gtcctgtcct catccctgaa accggcgaaa cgacgactct gcccggtgct
     1201 cctgcactgg gggatcgaaa ggtcgcggaa gatacggaac tatgctatca cttcttctcc
     1261 tgcttcaacg tcagggcagc agcggcgagc ggaatgggct caggggaaag aggagacctg
     1321 atgcagcacg gcaatcgagt tcccggcgtt cgccgaatcg cggttctccg cgccaatgcc
     1381 atcggcgact tcatcttcac gctgcccgct ctggaggcac tgcgcgcggc ctaccctgac
     1441 gccgagatcg tcctgctggg caagccctgg cacgcagcct ttctcgccga tcggccggga
     1501 cccgtggacc gggtcatcgt catcccaccc tgccgcggcg tgggcgaact cgaggactac
     1561 cccaacgaca gcgagaccct cgaccggttt ttcgctgaag tagccgagga ggacttcgac
     1621 ctcgccctcc agttgcacgg cggcgggcgg tactccaacc cgttcgcgtg ccgggtcggg
     1681 gcgcgcgtaa cggtcgggct caagaccccg gacgccatgc cactggacca ctgggtgcca
     1741 tatgtgttct tccagaacga gattcttcgc tacctggagg tcgtctcgct tgtcggcgca
     1801 ccacccgtca ccttggagcc ccgggttacc gtcactgacg ccgatctcgc ggaagcggcg
     1861 cccgtcgttc cccatgacaa caggccactg gtcatcctgc accctggcgc aggagatccg
     1921 cgacgtcgct ggccaccgca gaagttcgct gccgtcggcg acgccctggc tgcctccgga
     1981 atgcgcatcg gcgtcatcgg caccggcgag gacgagcgtc acctggtcga tacggttgtc
     2041 ggcaccatgc gcgccgacgc agtgaacctg tgggaaaaac tcacgctcgg tgggctggcg
     2101 ggactcctca gccgcagtcc cctcatcgtt tcgaacgact cagggccgct gcatcttgcc
     2161 gctgcggtcg gcgccgccac tgtcggcatc tactggtgct tcaacctcat caacgccggt
     2221 gccctgactc gcgcacggca ccggccgtta ctcgcctggc ggctggaatg cccggcttgc
     2281 gggctcaact gtctggaggg tcagtgtgac caccaggact ccttcgtcag cgatgttccg
     2341 gtcgaggacg tgatcgacca ggcgaccgag ctcctcgctc agtcctcgcg ccaataggcg
     2401 gcgtgtcgct cgcgaaactt ctcgacggtg atgccgtact ccagcatgtc gctatgctca
     2461 ccgtgggtga acacggtccg gcgccggcga ccttcgagct gaaagccgag cgcctcatgt
     2521 agccgctgtg acggctcgtt gatcgccatc acgtccacgt tgcacttctg ataccgcagc
     2581 tcttgaaaga agtagcggag gatcaggcaa atcgcgtcct tcgcataccc cttgcctcgg
     2641 tacgaagcat cgacgtggag tccataggag aagacgccgg cgcgagcgtt gcagtggtgc
     2701 gtggcaatgc tgcccacgag cgtgccttcg acgagggttt cgatctgcgc gatgaactca
     2761 tcgccctgcg gaccctcgac cgacgctgcc tcgacccact tcctcccggc tccactccgc
     2821 gcagacggat cagcctgcct ttatttcgcc atcaggacga ctttccttcc cggatcgacg
     2881 aggtgtacgg gcgggcatgg agcccgtccg tgttctcgct accgccaggc gaccgtgacc
     2941 tcctcgggat caacgacgct cgacgcgtct cgcacgacaa tcgtcttcga cctgccccgc
     3001 gcgatgtcga cgtagttgtc actgtagcgt gtcgtggcga caggccgctg caggtgcacg
     3061 aaccagcagt agccgtcctc cggcgcggtc aggatcacct ccagctcgtg atcactcacc
     3121 tgccgtttct cgaccttcgg ctcggctggc cgccggtcca gatccttgag atcgacgaag
     3181 aagtgccggt tgtccgggta gcgatcgccg gggctctcga cgcgcaggta ccggttgctg
     3241 gcgatgacgt cgctcggcca gacatccaca cgctcactgg agttcggcgg caggtcgacg
     3301 tagagctgtc gctcctccac cacctccccg ccgaaggtgc cgagcgtgat ggtcacctcg
     3361 tcctcgatgc gctccagcgt gtcgttggtg atccagagct cgacgccgtc gtctgtctcc
     3421 ttgaacgagg cgagcaccgg gctgtagacc cgcctcagat agtggtagcc cgccttcccg
     3481 aagccgtaat agtccatcac cgcccagctc agcacgggcc agcagtcgtt catctgccag
     3541 accagcgtac ctgagcagtg cggcttgcgg cggcgatagt gctcgatacc gaacttgagc
     3601 ccctccgcct gcgcgatctg gctgaagtcg atgtagtcct cgaggttctc agacacgccg
     3661 gtggtcgccg ccatcagcat gtcgcccttg tccttcgggt tgtccttgtt gtgccagtcc
     3721 atcgccgggc tgtggtgata gagctcgtcc tccggcacga cacgccgcag ggtctcgatc
     3781 accggcgagg catgcatccc gaactcggag cagaagcggg tcatgtcctc cgcgtaccgg
     3841 cggtagctga ccatctccgg cgtgaagatc tgctccggct tctcgccgaa tcgacgagga
     3901 ctcacctggc cgtgccagac atcccagttg tggcggttac cgtcctccat gctgttgtga
     3961 tcattgcctc catagggcga tcccggccag tagggcgtgc gtccgtcaag ctcgtagacc
     4021 gcatccggca ggatatggtt gtagtagaga gccccccagg gcggcggatc ctcgacgttc
     4081 cagtaggtct tgtcgttgat ccactggttc tcattgttgc cgacccagac cgccagcgac
     4141 gggtggctgc gcagccgccg gacctgatag cgagcctcgc ggtcgacctc ctgcacgaag
     4201 gtcggatcgt cttcagggta ggcggcacag gcgaacatga agtcctgcca gacgaggatg
     4261 ccgacccggt cgcactcgtc gtagaagcga tcgtgctcgt agatgccacc gccccagacg
     4321 cgcatcatct gaatgttcgc gtcgcgggca tcctcgagcc agcgggtgta gcgctcgtcc
     4381 tcgatcgcgc cgacgaagga gtcgggcggg atccagtctg cccccttggc gaagatcggc
     4441 actccgttca gcacgaagcg gaagaagcgc gcgcccttct cctcaaggtc tggcgactgg
     4501 tcgagcacca gcgagcggat cccgaccttg cgctcgtcgg tcacgacgac gtcgtcgcca
     4561 gcgatcagct cgacggtgag atcgtgaagg aatggctcgc caaggtcgtg cgtccaccag
     4621 agcttcgggt tgccgatggt gagataggca caaccatcgg cgtgtccggc tggcgactcc
     4681 gtgagatcga gctcggcagt ccactcgcgg tcactcgccg gctcgcgcag ggtgactcgg
     4741 gcgctgagcg gatcggtgcc agcgacacgc tccgcctcga cccggacggc gacaacggct
     4801 tgggtgtgat ccggtgagag gtcgacgttg tcgaagtgga tgccgaggat catcccccgg
     4861 tcgacgcgcc gtagctcaat gggtcgccac gtgccgacgg ttggcaggcg tggcccccaa
     4921 tcccacccgt agccaaactg ggcttttcgc atcgcggtcc gctcgcgtcc attcgcggcc
     4981 cattcgccaa ggtgacggcc ctcgatagcc agcagcggcg ggtgaaactg caacgcgagg
     5041 gtatgctcgc ggcccgcctg tacgtgctcg gtgatgtcga ccaccgtgtc gcggaacatg
     5101 ttctgactcg tgccgatctc ctcgccgtcc aagtagatgg tcacgaaagt gtcgagcccg
     5161 tgaaagacga gctgccagcg ctcgtgctgg ccgagttcga catcaggcac gtggagcggc
     5221 acgtgatacc accactcctt ctcctccatc caggcgcagt ccttctcctg ctggtcccag
     5281 aagggatcgg gaatctcgcc agcatcaatg agcgtgcgat ggacgtcgcc cggaatggtg
     5341 atctccagcc atccagtgtc gtcgaaatcg cctgccgctg cccccgcctc gacccccttg
     5401 ccgggatcga agtgcgccag cttgccactg ctcagaaacg tggacgcgtt gctcggtgtg
     5461 gtcgtgggca cggcggtgtc ccctctcgcg ctcagtgcga ctggtcatct gcgcctgagt
     5521 gtagcaagga ctggaccacc gtgggagcgc ttccacac
//
LOCUS       DASYWL010000155        10504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_607778, whole genome shotgun sequence.
ACCESSION   DASYWL010000155 DASYWL010000000
VERSION     DASYWL010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10504
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607778"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(287..1237)
                     /locus_tag="VGR22_05495"
     CDS             complement(287..1237)
                     /locus_tag="VGR22_05495"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEV2128057.1"
                     /translation="MRYSELVAPRRSEVAEGPTGEPGLGEVLIRVSTCGVCASELHPW
                     NDGRIPYSERLGHEPVGVVEKVGPGVTRFSPGDRITGLFEDAYSDFCIAPEDRLLPVP
                     RDVPDENALGEPLACLVNIQRRTRVELADRVALIGLGYMGLGTLQLLKLRGASRIVAI
                     DVREEARQRAVALGADEVYHPSDIPDEYLLTQFQDWEGDRGMDVVVEASGTPSGLTLA
                     GKMVRAHGVLSIIGFHQGGDRQVDVEMWNWKAIDVVNAHVRRREDLLESMRIGLDLEA
                     KGLIDLGSLVTHRFSLDEVDQAYTTLRDKPPGFVKAVITI"
     gene            1840..2793
                     /locus_tag="VGR22_05500"
     CDS             1840..2793
                     /locus_tag="VGR22_05500"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEV2128058.1"
                     /translation="MEVFGIEFVGVNVDNARKLLLSVVFVLLVLGLQGLLRWTLRGLY
                     MRRPSRRLRFWTRQGVAIATVLLLTIGLLSIWFEQPARLAAALGIIGAGLAFALQKVV
                     TALAGYFVILRGELFTVGDRITMGDVRGDVISVDFLTTHIFEMGQPPAEGQVPPTTWV
                     RSRQPTGRIVSVSNARVFDDPIYNYSQGFEYIWEEISVPIPYTADRDRAERILLEAAR
                     ERTGGLIEDASDTVARFARWHMAEHQPVEPKVYYRLTDNWLELTVRFIAPIRGVRDLK
                     DEISRDILREFDVAGIELAPSRLEILGGLESRRGQRDGARL"
     gene            complement(2891..3241)
                     /locus_tag="VGR22_05505"
     CDS             complement(2891..3241)
                     /locus_tag="VGR22_05505"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2128059.1"
                     /translation="MQLSIIVYVRDTERSAAFYESLGFERQGSVRPRWAAFTFGETLF
                     ALHGTMTGELDPPSDRVTINIVVSREELDRLHALCEERGYEITGPIDDVGFGPFFRVL
                     DPDGLPIQFNTRTR"
     gene            complement(3295..4326)
                     /locus_tag="VGR22_05510"
     CDS             complement(3295..4326)
                     /locus_tag="VGR22_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEV2128060.1"
                     /translation="MPDRIIASTAPEWREAAREMLADSLPDARFTAIGEDVLAISAEG
                     LAIADVAEIVRAERNPFVRHLFREVGRLDVPARQPVEETADAILTAWGDAPTPMPPAS
                     SLQVWTSGTVNLAFRPDTLRRELETALTEEGVRVARAGMAQVLSVCIAGNQVLVGLNG
                     AANALSDWPGGRVRLAKPKGQISRSEFKLEELFRTTDIRLPERGRVLDLGASPGGWTR
                     ILREHGLEVVAVDPADLDQRLRGDRGIRHVRTTAGPFLAETQDRFDVIVNDMRMEPEM
                     SVGLMVEAAERLVPGGMLIVTLKISPHDAVRRIRAALRRLDSAVDVIFARQLHHNRNE
                     ITVVGVKPA"
     gene            complement(4319..5164)
                     /locus_tag="VGR22_05515"
     CDS             complement(4319..5164)
                     /locus_tag="VGR22_05515"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2128061.1"
                     /translation="MGIPDQLASIHKLYGEIWASTDPDFETEIAKPRAPRSPELLFDY
                     AAQLGLGPDSHVLDIGARDAVHAIELARRHGCHVVAADPVPFHLGLARQRLEEAQPEV
                     QALVELSDATMEHMPFEDESFDLVWARDMLNHVALRRGLGEAYRVLKPGTPMLVFQTF
                     ATPLLEQGEARRLYEIHSVIAENMDSEFLEDVTRTTGFEIETVDEVGSEWREYRVESG
                     MDDIGQELMTLARMRRTEHELVQRFGRNRYEATIADILWGVYLLLGKLSSRIYIFRKP
                     GSIDA"
     gene            complement(5171..5422)
                     /locus_tag="VGR22_05520"
     CDS             complement(5171..5422)
                     /locus_tag="VGR22_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128062.1"
                     /translation="MSPFFRLVADQNQLTDAMYEFVTEPTPRRNHFPVIEELGDINQD
                     LVDVLEMMVIDGTDNRADVAAFVDFVFGPHGIGPIKHES"
     gene            complement(5855..6571)
                     /locus_tag="VGR22_05525"
     CDS             complement(5855..6571)
                     /locus_tag="VGR22_05525"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEV2128063.1"
                     /translation="MMPLTDDNRGRRTAPIVTWVLIAINVLVWLYEVLLPEQDLRQFL
                     YDWGIIPNEISNGEGWITLVTSMFLHGGWIHVIGNMLFLWVFGDNIEDIMGHVRYLIF
                     YLLAGLAAGYAQVLSNPDSTIPLIGASGAVSGLLAAYLISFPHGRIVTLVTLGVFITT
                     IMLPAWIMIGYWVVLQVIQGAMSLGVDVDTGGVAWFAHIGGFVAGLLLVFLFRDKDRV
                     EYQRAARARPEPRDRRAVFG"
     gene            complement(6584..6772)
                     /locus_tag="VGR22_05530"
     CDS             complement(6584..6772)
                     /locus_tag="VGR22_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEV2128064.1"
                     /translation="MLRNARTAVKFFTYGMIIGLLFAPQSGEQTRKQLFGMLGGTIEE
                     AVSNLTGGGSASGTSSQG"
     gene            complement(6819..7649)
                     /locus_tag="VGR22_05535"
     CDS             complement(6819..7649)
                     /locus_tag="VGR22_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128065.1"
                     /translation="MARKRHTGAFVAGSLLGGLAAAALTLWKTPKSGAELRASLPGVG
                     GETAHTVTTSETTRTVTASETANSERRFSNPVLSFVEKVVAPIPGVELGKLAKDEPGT
                     VSTEPVRTSVADTRQPGSAFEPREPAHGAAMATTETAAAASGERVEPVGGETVTERSG
                     AKVVRVDRTAEEPEPRTETDTTVERVAPLREERVTEPVSDDTVVTDQEPVGQKAVNPD
                     EEATHDPVEGSEAPAATTEELTHPTPEYVEQLEEQHDVEPVDDGSADFPEPPSRDDIR
                     "
     gene            7843..9072
                     /locus_tag="VGR22_05540"
     CDS             7843..9072
                     /locus_tag="VGR22_05540"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEV2128066.1"
                     /translation="MTVTTQPRPAPDEIGHPDVVEALRAEIAREGAITFARFMEQALY
                     HPDGGYYRTPARRPGRGGDFLTAPEVHPFFGITIANQVAELWERLDRPDPFVVREYGP
                     GVGGLAYDIIAGLHAHHPEVIPALRYRLRDVNRHRMAEAMSAMMEVGLDDIVSVENPE
                     HEETIEPVTGVVLANEVADALPAHELVWTGDALREHWVVWDPATDWFAWEVRDLSPEV
                     AATDPLDWLRGRDIDPTRWPAGSRIAWSPALTDWIGEIGRGLDRGYALIIDYGYPALE
                     LYRDHLLEGTIRAYREHTVSDDPFQHVGEQDLTVHVDFTRITDAAEAMEIAATPVVSQ
                     GDFLSRLEMGQLLVGLQGDPEIDMLGYHRAQAAVFRLIDPGGLGRFRVVGLAKNAPLD
                     PLPTGFTPDKLPESLRI"
     gene            complement(9344..10177)
                     /locus_tag="VGR22_05545"
     CDS             complement(9344..10177)
                     /locus_tag="VGR22_05545"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV2128067.1"
                     /translation="MRTDGFRHAFGRVLLHVVLMTTGIFFMFPFIWSISTSLKPMADL
                     FSVTPSLIPSEFRWQNYADVFDSAPFLRFYLNTVIVTIVRTAGQVFIASLAGFAFAQL
                     RFPGRGALFFLLLAGLMVPDQVLIVPRFIVMREFGWLNTYQGLIIPLTFSAFGAFLLR
                     QYFLGIPKDFHEAARLEGANPFQVYRDIYLPLARPALGAFAFLSLLFSWNEFLWALTV
                     TNSADMQVLSVGIALFQGQHFTNNAILLAAANMATIPLLIAFLFFQKQLVEGITLSGL
                     K"
     gene            complement(10174..>10504)
                     /locus_tag="VGR22_05550"
     CDS             complement(10174..>10504)
                     /locus_tag="VGR22_05550"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV2128068.1"
                     /translation="DYYHAAALDGASSWQKFRDITFPLLTPTTFFLMVIGVIFSLQVF
                     DLVYVMTRLDQSNRLPTLVYYIYQEGFRNFRMGYAITVAWVLLAMILVFTLVQFRLQR
                     RWVHYET"
BASE COUNT         1846 a         3390 c         3454 g         1814 t
ORIGIN      
        1 atgcgggtcc aaaagatctt cagaaaccaa tggcttgctg ataccacgac gaccacaacc
       61 ccggttccac agttttgtta gaggctctct tgtctaagag aacagccagc gcttcacgtg
      121 ttcgaactcg gacacagaga gggcaagatc gcctgatgtg ggcactgcta gtgtcagctg
      181 cgatgggttt gaggcgagca acatcggcgt ggaaccagcc atggttcgcc catgaacccg
      241 gcatgttccg tgttgcggca aagcgaacag ctgctttctc gcagcctcag atcgtaatga
      301 cggccttcac gaagcccggc ggcttgtcgc gcaaggtcgt gtatgcctga tcgacctcgt
      361 cgaggctgaa tcgatgggtc accagactgc ccagatcgat caatcccttt gcctcaagat
      421 cgagaccgat acgcatgctc tccagaagat cctcgcgtcg ccggacgtgc gcgttgacaa
      481 cgtcaatcgc cttccaattc cacatctcta catccacttg gcgatcgccg ccctgatgga
      541 agccgatgat ggagaggacg ccgtgtgccc ggaccatctt tccggccagc gtcaatcccg
      601 acggggtccc agatgcttcc accacgacat ccatgccccg atcgccttcc caatcctgga
      661 attgcgtcag cagatactcg tccgggatgt ctgacgggtg gtacacctcg tccgcgccaa
      721 gtgccacggc cctctggcgg gcctcctcgc gaacgtcaat ggcaacgatc cgactcgctc
      781 cgcgaagttt cagtaattgg agcgtcccca agcccatgta gccgagcccg atcagggcga
      841 cgcggtctgc aagctcgacg cgcgtgcgtc gctggatatt caccaaacac gccagtggct
      901 caccaagggc gttctcatcc ggcacatccc ttggcacggg aagcaagcgg tcctcgggag
      961 caatgcagaa atcgctgtag gcatcctcga acaacccagt gatccgatcg cccgggctga
     1021 agcgagtcac gcccggcccc accttctcga cgactccgac cggttcgtgg ccgagccgtt
     1081 cagaataggg tatccgccca tcgttccagg ggtgcaattc cgaggcacac acgccacagg
     1141 tgcttacccg aatcaggacc tcgcccagtc ccggctctcc tgttggtccc tcggccactt
     1201 ccgaccgtcg gggagcaacc agttcgctgt accgcatctc tcgttcctcc gttgaaactg
     1261 aaggtgaccc agagccgcgg gggcccgacc gggtcagacg ccgagggcgt aggcattcac
     1321 gcctccaaga tacccgcgct gagacgcgag gaaaagcacc acgtgcgcga ccgagttagg
     1381 cggcgatatc ctcgagcatc gatcaagccg gttaatgcgc cagcattgga cacaaatcga
     1441 tgcaaatcgt gttgttccac cgaatcgggt ggcggtgatt gctgcggccg aatccgctcg
     1501 tcgagaggcg ggactttatg gtcgtcccgt tctcggggca tacgtccgac cccttctggt
     1561 ggacagggaa agatgtgttg tcgtcgcagt gccgcactgc gcatgtgcct tgggcacctc
     1621 ctatcccggc agacccgcac cggcctctgc accatcaagg gcaaccgggg ccaaggggca
     1681 taatccggcg gttcaccatt gatgaagatc gtcggcagcg ggagccgtgc ttgcctgccg
     1741 ttgccgtgga cagggctggc aagcggtgtg cggagcgtct tcccgatacg gagtgccaac
     1801 ccgccgggtc gggcgcgcat gcatcggaga cctgctggca tggaggtgtt cggaatcgaa
     1861 ttcgtcggcg tcaacgtcga caacgcacga aagctcctgc tgtcagtggt tttcgtgctg
     1921 ctcgtgctgg gactgcaggg gctgctccgc tggacgctgc ggggtctcta catgcgccgg
     1981 ccgtcgcggc gcctccgctt ctggacgcgg cagggggtcg ccatcgcgac cgtgctgctc
     2041 ctcacgatcg gcctgctctc catctggttc gagcagccgg cgcgtctggc ggctgcgctc
     2101 gggatcatcg gcgccgggct ggcctttgcc ctccagaagg tcgtgacggc gctggcgggc
     2161 tacttcgtca tcctccgtgg ggagctcttc accgtcggcg atcgcatcac gatgggcgac
     2221 gtgcggggtg atgtcatcag cgtcgacttc ctcaccacgc acatcttcga gatggggcag
     2281 ccaccggcgg aggggcaggt gccgccgaca acctgggtgc ggagccggca gccgaccggg
     2341 cggatcgtca gcgtcagcaa cgcgagggtc ttcgacgacc ccatctacaa ctactcgcaa
     2401 ggcttcgagt acatctggga agagatttcg gtgccgatcc cctacacggc cgaccgagat
     2461 cgcgccgagc gtatcttgct ggaagcggcg cgggagcgga cgggtgggct cattgaggat
     2521 gcgtccgaca ctgtggctcg cttcgcccgc tggcacatgg cggagcacca gcccgtcgag
     2581 ccgaaggtct actaccggct gaccgacaac tggctcgagc tcaccgtgcg cttcatcgcg
     2641 ccgattcggg gcgtgcgcga cctcaaggac gagatcagcc gcgacatcct ccgggagttc
     2701 gacgtggccg gcatcgagct ggcaccctcg cggctcgaga tcctgggcgg gctggagtcg
     2761 cgacgtggcc agcgagacgg tgcgcgcttg tagcgcgcgt tgccggtgtg ggagtgcaga
     2821 agtcaggcca tccgcttgga gcgccggccc gaacgcgggc ggacacaaga agctgacctg
     2881 agcgagcgcc tcagcgcgtg cgggtgttga actggatggg caggccgtcg ggatccagca
     2941 cgcggaagaa tgggccgaac ccgacgtcgt cgattggccc ggtgatttcg tacccccgct
     3001 cctcacagag cgcgtggagg cggtccagct cctcacggga gacgacgatg ttgatcgtca
     3061 cccggtcgct gggcgggtcg agctcgccgg tcatcgtgcc gtggagggcg aagagcgtct
     3121 cgccgaacgt gaaggcggcc cagcgtggtc gcaccgaccc ctgccgctcg aacccgagtg
     3181 attcatagaa ggcggccgag cgctccgtat cgcgaacgta gacgatgatc gagagctgca
     3241 ttgttgagta cctctctgac tggtgcctga tcggcggacc aatggagcag cgagtcaggc
     3301 cggcttgacg cccacgacgg tgatctcgtt gcggttgtgg tggagctggc gggcgaagat
     3361 gacgtcgacg gcgctgtcca gccgccggag cgcggcccgg atccgccgga cggcgtcgtg
     3421 cggcgagatc ttcagcgtga cgatcagcat gccgcccggc accagccgct ccgccgcttc
     3481 caccatcaac ccaaccgaca tctccggctc catgcgcatg tcgttgacga tcacatcgaa
     3541 gcggtcctgc gtctcggcca gaaatggacc ggcggtggtg cggacatggc ggataccgcg
     3601 atcgccgcgc aggcgctggt cgagatcggc cgggtcgaca gccaccacct ccagcccgtg
     3661 ctcccgcagg atgcgcgtcc atccgccggg gctggcgccg aggtcgagca cccggccccg
     3721 ctccggcagc cgaatgtcgg tcgtgcgaaa cagttcctcc agcttgaact cagagcggga
     3781 gatctgcccc ttcggcttcg cgagccgcac cctgccgccg ggccagtcgc tcagggcatt
     3841 cgcggcgccg ttcagcccga ccagcacttg gttgccggca atgcagaccg agagcacctg
     3901 cgccatgccg gcccgggcga cccgaacgcc ctcctcggtc agggctgtct ccagctcgcg
     3961 gcgcagcgtg tctggacgga aggcaaggtt gactgtcccg ctcgtccaga cctgcagcga
     4021 cgacgcgggc ggcatcggcg tcggcgcgtc gccccacgcg gtcagaatgg catcggcggt
     4081 ttcttccacc ggctgtcggg ctggcacatc gagccgcccg acctcacgga agaggtgacg
     4141 cacgaagggg ttgcgctcgg cgcggacgat ttcggcgaca tcggcgatgg cgagcccctc
     4201 ggcggagatc gcgagcacgt cctcgccaat agcggtgaag cgcgcgtccg ggagcgaatc
     4261 ggcgagcatc tcgcgggccg cctcgcgcca ttccggtgcc gtcgaggcga tgatccggtc
     4321 aggcatcgat cgatcccggc ttgcggaaga tgtagatgcg ggagctgagt ttgccgagca
     4381 ggaggtagac cccccagagg atatcggcga tggtcgcctc gtagcggttg cgtccgaagc
     4441 gctgcaccag ctcgtgctcg gtgcgacgca tgcgcgcgag cgtcatcagc tcctggccga
     4501 tgtcgtccat gccggattcg acgcggtact cgcgccactc gctgccgacc tcgtcgaccg
     4561 tctcgatctc gaaaccagtc gttctggtaa catcctcgag gaactcggaa tccatattct
     4621 ccgcgatgac cgagtggatc tcgtagagcc gccgggcctc gccctgttcc agcagcgggg
     4681 tggcgaaggt ctggaagacg agcatcggcg taccaggctt caacacccgg tacgcctcac
     4741 cgaggccgcg gcgcagcgcg acatggttga gcatgtcccg cgcccagacg aggtcgaacg
     4801 actcgtcctc gaagggcatg tgctccatgg tggcatccga cagctcgacc agcgcctgga
     4861 cctccggctg tgcttcttcc agccgctgcc tcgctagtcc gaggtggaag ggtaccgggt
     4921 cggcagcgac gacgtggcag ccgtgacgac gggcaagctc aatggcgtgg acggcgtcgc
     4981 gcgcgccgat atcgagcaca tgcgaatccg gcccaagacc gagttgcgcg gcgtagtcga
     5041 aaagcagctc gggggaccgc ggcgcgcgcg gtttcgcgat ctcggtctcg aagtcaggat
     5101 cggtgctggc ccagatctcg ccgtagagct tgtggatgct ggcgagctgg tcggggatgc
     5161 ccatctcggc tcaggactca tgcttgatgg ggccgatgcc atggggaccg aagacgaagt
     5221 cgacgaaagc ggccacgtcc gcgcggttgt cggtgccgtc gatcaccatc atctccagca
     5281 cgtcgacgag gtcctgattg atgtcgccga gctcctcaat gaccgggaag tggttgcggc
     5341 gcggggtggg ctcggtgacg aactcgtaca tcgcgtcggt aagctggttc tggtcggcga
     5401 cgaggcggaa gaatggggac atggaagata cgctcctgtg ggacggaaat cctatctcat
     5461 catggcacac aagtcgcaca gccggtgccg ccgttacgct gagatgcatg cggggtgtga
     5521 ctgaccgtat accgtgtgat ggaggtgggc aatctgcagg ggcgacaccg ctgcggtcgc
     5581 ccagtgggcg tgcggggcgc tcaatgccgt gcaagcggtc acagatgtca tcccaatccg
     5641 gcgaggcgcg ccagcgccga gacgtatcga ggaatctccc gccggtccgc ctttcttctc
     5701 ttcgctcagg gcagctcttc gattccggcc ttcgctgagg atgacaccgt ttccgatggc
     5761 cgttggagcc cgttcgttgc tctccgggac gaacttgggc ggacgccgca aggacgtccg
     5821 cccgttcccg acggtatcga tgtatcgtcc gacctcagcc gaagacggcg cgccggtcgc
     5881 gcggctccgg acgggcgcgg gcggcccgct ggtactccac ccgatccttg tcgcggaaga
     5941 ggaagaccag cagcaacccc gccacgaagc cgccgatgtg cgcgaaccat gccacgccgc
     6001 cggtgtcgac gtcgacaccg agcgacatcg ccccctggat cacctgcagc acgacccagt
     6061 acccgatcat gatccacgcc ggcagcatga tggtggtgat gaagacgccc agcgttacca
     6121 gcgtcacgat gcgaccgtgc gggaagctga tgaggtaggc cgccaggagc ccggagacgg
     6181 caccgctcgc gccgatcagc ggtatggtac tgtccgggtt gctgagcacc tgcgcgtagc
     6241 ctgccgcgag gccagccagg aggtagaaga tcaggtagcg cacatgcccc atgatgtcct
     6301 cgatattgtc gccaaagacc cacaggaaga gcatgttgcc gatgacgtgg atccacccac
     6361 catgcaggaa catgctggtc accagtgtga tccagccctc gccattgctg atctcgttcg
     6421 gaatgatgcc ccagtcatag aggaactgcc gcagatcctg ctccgggagc agcacctcgt
     6481 acagccagac cagcacgttg atggcgatca gcacccacgt gacaatcggg gccgtgcgcc
     6541 ggccccgatt gtcatctgtc agcggcatca tggctcagcg ctcttatccc tggctgctgg
     6601 tgccgctggc gctgccacca ccggtgaggt tgctcacggc ctcctcgatc gtgccaccca
     6661 gcatcccgaa gagctgcttg cgggtttgct cgccactctg cggggcgaac agcagcccga
     6721 tgatcatgcc gtaggtgaaa aacttgaccg cggtgcgtgc gttgcgaagc atgaactttg
     6781 tcctttcgat acgataattc tcggcctgcc caagtggatc agcggatgtc gtcacgcgag
     6841 ggtggctcgg ggaaatccgc cgagccgtca tcgaccggct ccacgtcgtg ttgctcttca
     6901 agctgctcca catactccgg tgtcgggtgg gtgagttcct ccgtcgtggc ggcaggggcc
     6961 tcgctgccct ccaccgggtc gtgtgtcgct tcctcatcgg ggttgacggc cttctgcccg
     7021 acgggctcct gatcggtgac gacggtgtcg tcgcttaccg gctcggtgac gcgctcctcc
     7081 cggaggggcg ccacgcgctc cactgtcgtg tcggtctcgg tacggggttc cggctcctcg
     7141 gctgtccggt cgactcgtac caccttggcg ccggagcgct cagtgacggt ttcgccgccg
     7201 accggttcca cccgctcgcc agacgcggca gccgcagttt cggtggtggc catggctgcc
     7261 ccgtgcgccg gctcgcgtgg ttcgaaggcg gatccgggct ggcgggtatc ggcgaccgag
     7321 gttcgcactg gctcggtgga aaccgtccca ggctcgtcct tggcgagctt gcccagctcg
     7381 acgccaggga tgggcgccac caccttctcg acaaagctca gcaccgggtt ggagaaccgg
     7441 cgctcactgt tggcggtctc ggatgccgtc acggtgcggg tggtctcgga ggtcgttacc
     7501 gtgtgggcgg tctctccacc gacgcccggg agtgatgcgc gcaactcggc gcccgatttc
     7561 ggtgtcttcc agagcgtgag cgccgcggcg gccaacccgc cgagcaggct ccccgcaacg
     7621 aacgcgccag tatgtcgttt tctggccatc gaactgccct ccttaagaca ttccggggct
     7681 ccggatggaa ccgagcggag tcgcatcccc agacttcatg gacatattgt ggggtatctg
     7741 gcacatcgcg tgccacgggt ccgccgctgg cgttgggtaa actcgcgctg accttccggg
     7801 cgaggactcg actcgtaccc tcagatggag catacgcttg gcatgacagt caccacccag
     7861 ccccggcccg ctccggacga gatcggccat cccgatgtcg tcgaggcgct ccgcgcagag
     7921 atcgcccgag agggcgcgat caccttcgca cgcttcatgg agcaggcgct ctaccatccc
     7981 gatggcggct actaccgcac acccgcccgg cgacccggcc gtggcggtga tttcctcacc
     8041 gcgccggagg tgcatccctt cttcggcatc accatcgcca atcaggtcgc cgagctgtgg
     8101 gagcgcctcg atcgccccga cccgttcgtc gtgcgcgagt acgggcccgg cgtcggcggc
     8161 ctcgcctacg acatcatcgc aggtctccac gcgcaccacc ccgaggtcat cccggcgttg
     8221 cgctaccgcc tgcgcgacgt caaccggcac cgcatggcgg aggcgatgag cgcgatgatg
     8281 gaggtcggac ttgacgatat cgtctcggtc gagaatccgg aacacgagga gaccatcgag
     8341 ccggtcacgg gtgtggtgct cgccaacgaa gtcgccgacg cgctgccggc gcacgagctg
     8401 gtctggaccg gcgacgcatt gcgcgagcac tgggtggtgt gggatcccgc gaccgactgg
     8461 ttcgcgtggg aggtccgcga tctgtcgccg gaggtcgccg cgaccgaccc gctcgactgg
     8521 ctgcgcgggc gcgacatcga cccgacgagg tggccggcgg gttcgcgtat cgcatggtcg
     8581 cccgcactga ccgactggat tggggagatc ggacgagggc tcgatcgcgg ctacgcgctg
     8641 atcatcgact atggctatcc cgcgctcgag ctctatcgcg atcacctgct ggagggcacc
     8701 atccgcgcct acagggaaca caccgtcagc gatgatccgt tccagcatgt cggcgagcag
     8761 gacctgaccg tccacgtcga tttcacgcgt atcaccgatg ccgccgaagc gatggagata
     8821 gcggcgacac cagtcgtttc gcagggagac ttcctctcac ggctggagat ggggcagctg
     8881 ctggtcgggt tgcagggaga tccggagatc gacatgctcg ggtaccatcg ggcacaggcg
     8941 gccgtcttcc ggctgatcga cccgggcggt ctcggccgct tccgggtcgt cggactggcg
     9001 aaaaacgcgc cgctggaccc gcttccgacc gggttcacgc cggacaagct gccggagtcc
     9061 cttcgcatat gactggccaa cgcatgacga gggagcggcc atgaccgata ccgatgcccc
     9121 cacgccatcc tgctcggcgc cgacgtggca tccgagcgca cccgggccca tcccgtcctc
     9181 accggcgtcg atggaggggt gtcgtgaccg ggacgacatt gacggcgcgg ggcctcagcg
     9241 aggatgtcgt tggacgagcg gcgccaagtc gtcccctacg gggcgctgat cctgggaatt
     9301 aggtgttgat ggccatggta gccgctggga acgcgacttg gtgctactta agaccgctca
     9361 gggtgatgcc ctccaccagc tgcttctgga agaagagaaa cgcgatcagc agcgggatgg
     9421 ttgccatgtt ggccgctgcc agcaggatgg cattgttcgt gaagtgctgt ccttggaaga
     9481 gtgcgatccc caccgagagc acctgcatat ctgcgctgtt ggtgaccgtc agcgcccaca
     9541 ggaactcgtt ccacgagaag agcaggctca ggaacgcgaa cgccccgagg gccgggcgtg
     9601 ccagcggcag gtagatgtcc cggtagacct ggaaggggtt ggccccctcc agcctggcgg
     9661 cctcgtggaa gtccttcggg atcccgagga agtactgccg cagcaggaag gcgccgaacg
     9721 cgctgaacgt cagcgggatg atgagcccct ggtaggtgtt cagccagccg aactcccgca
     9781 tcacgatgaa gcgcggcacg atcagcacct gatccggcac catcaacccg gccagcagca
     9841 ggaagaagag cgcaccgcgt ccggggaacc gcagctgcgc gaaggcaaac ccggccagag
     9901 aggcaatgaa cacctgtccc gccgtccgga caatggtgac gatcaccgtg ttgaggtaga
     9961 agcgcaggaa gggggcgcta tcgaagacgt cggcgtagtt ctgccagcgg aattcgctcg
    10021 ggatgaggct gggcgtgacc gagaagaggt cggccattgg cttgagcgac gtcgagatcg
    10081 accagatgaa cgggaacatg aagaagatcc cggtcgtcat cagcaccaca tgcaggagca
    10141 cccggccgaa cgcgtgccgg aagccatcgg tcctcatgtc tcgtaatgca cccagcgtcg
    10201 ctgcaggcgg aactgcacca gcgtgaagac gaggatcatc gccagcagca cccacgcgac
    10261 agtgatggcg tagcccatcc ggaagttccg gaagccctcc tgatagatgt agtagacgag
    10321 cgtcggcagc cggttggact ggtcgagccg agtcatcacg tagacgagat cgaagacctg
    10381 cagcgagaag atcaccccga tcaccatcag gaagaaggtg gtcggggtca gcagcgggaa
    10441 ggtgatatcc cggaacttct gccagctgct ggcgccgtcg agcgcggccg cgtggtagta
    10501 gtcc
//
LOCUS       DASYWL010000156         2393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_44555, whole genome shotgun sequence.
ACCESSION   DASYWL010000156 DASYWL010000000
VERSION     DASYWL010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44555"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..708
                     /locus_tag="VGR22_05555"
     CDS             70..708
                     /locus_tag="VGR22_05555"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV2128069.1"
                     /translation="MHARWLLEDGHSVHLVDLAPRHVDHAVTQLGPSGLTAEVGDARQ
                     LSHPDDSVDAVLLLGPLYHLQDADDRATVLHEARRIVSPGGLVSVAAISRFASLFDGL
                     VRGYLFDDEFALIVDRDLQSGCHDNPDSRPHWFTTAYFHRPEELVQEVEAVGLQLVEL
                     VGIEGLAGWLPELEARWNDDTDRAVIVESARRIEAEPALLGLSAHLLAVARG"
     gene            717..1352
                     /locus_tag="VGR22_05560"
     CDS             717..1352
                     /locus_tag="VGR22_05560"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEV2128070.1"
                     /translation="MKLYLSSYRIGSEPERLRELVGPGRRAAIIFNACDCFEQRLKFY
                     EREEIDLADLGFEAEELDLRDYFGHPERLRLQLMTYDLLWVVGGNTFVLARAMNAAGF
                     AAAAGDLVRNDQLTYAGYSAGVCVTGPDLDGIHLMDHPETVPKGYGESLPTTTLGWVP
                     WRIVPHWQSDHPEAEAAEVGARYMEERSLAYRPLSDGHAIVIAVPGPTTSR"
     gene            1949..>2393
                     /locus_tag="VGR22_05565"
     CDS             1949..>2393
                     /locus_tag="VGR22_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128071.1"
                     /translation="MRQRFVERHMAAATVFGTIAVILLLSAIGSVLADRISEWLAHGL
                     GHAVVALPVGALAVLARRTWPAPRALAPGRLARPIVVFGLAGLATGQLLEVLGARVDK
                     PNAHPIEEIAHTAGQIVTILSMPLLLTGVVMTAIAGARAGAVPRWL"
BASE COUNT          396 a          780 c          776 g          441 t
ORIGIN      
        1 caagaagtct tgcgccgcca cttggggccg ccggcgcagc gcgttctcga cgtgggcggc
       61 ggtaccggcg tgcatgctcg ctggttgctc gaggacggcc acagtgtcca tctcgtggac
      121 ctcgctccgc gccacgtcga tcatgctgtg actcaacttg gtccctccgg tctcacggct
      181 gaggtgggcg acgctcggca gctctcccac cccgacgatt ccgtcgatgc ggtccttctt
      241 ctgggcccgc tctatcacct tcaagacgcg gacgatcgag cgaccgtcct gcacgaggcg
      301 cgcaggattg tgtctccggg agggttggtt tcggttgcag cgatcagccg cttcgcgtcg
      361 ctgttcgatg gtctcgtacg tggctatctc ttcgacgatg agttcgcctt gatcgtggac
      421 cgcgacctcc agtcaggctg tcatgacaat ccggatagcc ggccgcactg gttcacgacc
      481 gcgtacttcc atcgcccgga ggaactggtg caggaggtcg aggcggtcgg ccttcaactt
      541 gtcgaactgg tcggcataga agggctcgct ggctggctcc ccgagctcga agcgaggtgg
      601 aacgacgaca cggatcgagc ggtgatcgtc gaaagcgccc ggcgaatcga ggcagagccg
      661 gcgctgctcg gtctcagcgc gcatctcttg gccgtggctc gcggctgacc gctccgatga
      721 agctctacct gagttcctac cgaatcggta gcgaaccgga gcgcctgcgg gagctggtgg
      781 gaccaggccg ccgagccgcg atcatcttca acgcatgtga ctgcttcgag caacgcctca
      841 agttctacga gcgggaggag atcgatctcg ccgacttggg tttcgaggca gaagaactcg
      901 atctgcgcga ctacttcggg cacccagagc ggctccgatt gcagctcatg acctacgacc
      961 tcctgtgggt tgtcggaggc aacaccttcg tgctcgctcg agcgatgaat gcagctggct
     1021 tcgctgccgc cgcgggggac ctcgtgcgca acgaccaact gacatacgcg ggctacagcg
     1081 ccggggtgtg cgtcacggga cccgatctcg acggcatcca cctgatggac cacccggaga
     1141 ctgtgcccaa agggtatggc gaatctctcc cgacgacgac cctcggctgg gtgccgtggc
     1201 gaatagtgcc tcactggcaa tctgaccatc ccgaagccga agcagcagaa gtcggcgccc
     1261 gctacatgga agaaaggtcc ctcgcatacc gccctctcag cgacggccac gccattgtca
     1321 tcgccgtgcc agggcccacc acctctcggt agtcgtaggt ggacatcggt cgagcgcgag
     1381 aacatcgaac gtgtcccaca acgcgctgcg acgccggcct gcgcggctag caccaaaccc
     1441 gcggggcgcc gatgtgccgc tcggcgtggg tccggcacct gagcgcaacc aggacggagt
     1501 cgacgcctag cgaccaccga tcattcctta gcgttggcct cggcggccgg tgagccggca
     1561 ttcatcgcgc ttcgtccagg ccgggatcgg gtcagcgatg cgccgttctg ttcctcgccg
     1621 acggttgact tattcgcgac tcggtttcgc cgataggtgc ggccgcgacc gggaccggtg
     1681 agcacggcgg cacgcccggc cgcctgctga acgccttgga cgaccttgtc gccggtgccg
     1741 caacgacgat cgggacactc acgcggtcgg cttccgccgc tccaaaaact gcgcaaccgg
     1801 gatcacctgg gaacgcaggc gtccccggcc ctccgctacg aacgccgaca cgaaaactcc
     1861 atactttgcg gagcgaggac aagcctccct cggatggcgc gacgatgaaa gcactcccat
     1921 tccatgactc ggaggtggag gaacctctat gcgtcagcgg tttgtcgagc ggcacatggc
     1981 ggcggcgact gtcttcggga caatcgctgt catcctcttg ctgagtgcca tcgggtccgt
     2041 cttggctgat cggatctccg agtggctggc ccacggtctg ggccatgcgg tggttgccct
     2101 accagtgggt gccttggccg ttcttgcgcg aaggacctgg ccagcgccca gagccctggc
     2161 tcctggccga ctcgctcgac ccatcgtggt gtttgggctc gccgggttgg caaccgggca
     2221 gctgctggag gtactgggtg cgcgcgttga caagccgaac gctcatccga tcgaggagat
     2281 cgcgcatacg gccgggcaga tcgtgaccat tctttcgatg ccgctgttgc tcaccggtgt
     2341 ggtcatgacg gcgatagctg gcgcgcgagc gggcgctgtc ccaagatggc tag
//
LOCUS       DASYWL010000157        19462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_7771, whole genome shotgun sequence.
ACCESSION   DASYWL010000157 DASYWL010000000
VERSION     DASYWL010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 19462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19462
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7771"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="VGR22_05570"
     CDS             complement(<1..142)
                     /locus_tag="VGR22_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEV2128072.1"
                     /translation="MAKQKFERTKPHVNVGTIGHVDHGKTTLTAAITKTLALAGGAGT
                     FRD"
     gene            complement(247..2358)
                     /gene="fusA"
                     /locus_tag="VGR22_05575"
     CDS             complement(247..2358)
                     /gene="fusA"
                     /locus_tag="VGR22_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEV2128073.1"
                     /translation="MAGTATQPNLADPQVALRRTRNIGIIAHIDAGKTTTTERILFYT
                     GIVHKVGETHEGAAVMDWMAQERERGITITSAATTCFWRDHRINIIDTPGHVDFTVEV
                     ERSLRVLDGGVVVFDGVAGVEPQSETVWRQADKYNVPRICFVNKMDRTGANFERTVEM
                     IVDRLKANPAPVQLPMGAEDTFVGVIDLINMKAITYHDDLGQNIEVGEIPAEFQEAAE
                     AARVELVEKVAELDEELMMKYLEGEKITSEELVAGLRAGTLNSTLVPVMAGSALKNKG
                     VQPMLDSIIDYLPSPLDVPPVKGIDPKTEEEITRTADPSAPFSALAFKIVSDPHVGKL
                     SYVRVYSGTMQSGSYALNSTKGRRERIGRLLQMHSNHREEIPEVSAGNIAAVIGLKDT
                     TTGDTLCDPDQQIVLESITFPEPVISVSIEPKTRADQDKMSNALQRLAEEDPTFRVKT
                     DEETGQTVIDGMGELHLDVIVDRMMREFRVDANVGRPQVAYRETLTREARVEGRHVRQ
                     SGGKGQYGHVIVEFGPRDRGEGYEFEDAIVGGAIPREYIPAVNAGIREAMDTGGKAGF
                     PVVDVKARLVDGSFHEVDSSEMAFKIAGSLAFKEALNRGKPVVLEPVMSVEVTTPEEF
                     MGDVIGDLNSRRGQIQGMEERAGAQVVRALVPLANMFGYVTDLRSMTQGRAVSSMEFD
                     HYAPLPDNLAQEFVEKATARS"
     gene            complement(2443..2913)
                     /gene="rpsG"
                     /locus_tag="VGR22_05580"
     CDS             complement(2443..2913)
                     /gene="rpsG"
                     /locus_tag="VGR22_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEV2128074.1"
                     /translation="MPRRAKIERRVPQPDARYNSELVTRFINKIMQRGKKGLAERIMY
                     DALQMIQDRTGNDPITVFEQAVQNATPMLEVKPRRVGGATYQVPVQIEGQRRQSLAIR
                     WLLASARARAGKTMQEKLANELLDAYNGTGATIKRREDTHRMAEANRAFSHFRF"
     gene            complement(3288..3713)
                     /gene="rpsL"
                     /locus_tag="VGR22_05585"
     CDS             complement(3288..3713)
                     /gene="rpsL"
                     /locus_tag="VGR22_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEV2128075.1"
                     /translation="MPTINQLVRKGRKSVKKKTKSPALRFTNSSAGRYAGTLRGDTFA
                     PQKRGVCTQVKTMTPKKPNSALRKIARVRLSNGVEVTAYIPGEGHNLQEHSVVLIRGG
                     RVKDLPGVRYHIVRGALDARGVENRKQARSKYGTKAPSS"
     gene            complement(3926..5119)
                     /locus_tag="VGR22_05590"
     CDS             complement(3926..5119)
                     /locus_tag="VGR22_05590"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEV2128076.1"
                     /translation="MTGLPLPIKKAVVLQWQGQELRFDVAQDLFSSQQIDKGSHVLLD
                     SLDPTLFPETGSAADFGCGYGVLGIAWQSSKPGWSMRYVDRDALAVTFSAHNARQLGD
                     AVTGFNIDFSLPATDSGYDLVLWNVPGKAGRPVIAGLLDVALGTVAPGGLLAVVVVHP
                     LADLFAEEFTGGDTTVELVEPGTEQTVVHLRRVAGKVTERDPFAEGLFDQPEARFVAA
                     DLEWHLIPVIGLPEYDELGHATKLAAELMRDLPAGVPPARWLIVDPGVGHLAVAASLL
                     WPGAHGVIAGRDVLALRSTTRAVAGTAVQAMPVWGVEALNLDVPVDVAILALPVQAST
                     DEIERGVDAVERCGAAYVHTILHGRSTEVARAERVLRRRSRWRAAKPIKQRGFAALAA
                     RTVVP"
     gene            5223..6257
                     /locus_tag="VGR22_05595"
     CDS             5223..6257
                     /locus_tag="VGR22_05595"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2128077.1"
                     /translation="MSKSLGIGIAGAGFIGKVQANALREIDGAEVVAIADPREDAGQA
                     LATATGARWYPSYTEMLEHPELDVVVLTTPSGLHAEQAVLAAQAGKHIITEKPMSTTP
                     HGIQLMTEAAERANVELAVIFQNRFSRDVIKVKRGIEAGMLGRPVMGSAAVHWHRTAE
                     YYEANDGWRGTWTLDGGGALMNQSVHTIDLLQWLMGGVSAVSANAATLIHEIETEDTA
                     TASVVFRSGALGTIAGTTSASTDAPVRVEIVGTGGRAMLERGVLVAWEGDSPLTDDIL
                     LPFDHDYIQGWEPGEPFGAAHKRQLAVILDALRSGDTPPVPPREARKAVDIVLAVYES
                     AQTGKHVAID"
     gene            6499..6837
                     /locus_tag="VGR22_05600"
     CDS             6499..6837
                     /locus_tag="VGR22_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128078.1"
                     /translation="MEYAIFTVSTPQFSPEVVVHRTKAAGYDGIEWRVKDDAPSDAPT
                     FWSGNRATLPLTGLEENAPTWRRMAEDVGLEMPAIASYVKCDELERVELAMRGAAALG
                     VPMLRIQLPT"
     gene            6877..7368
                     /locus_tag="VGR22_05605"
                     /pseudo
     CDS             6877..7368
                     /locus_tag="VGR22_05605"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
     gene            complement(7657..12213)
                     /gene="rpoC"
                     /locus_tag="VGR22_05610"
     CDS             complement(7657..12213)
                     /gene="rpoC"
                     /locus_tag="VGR22_05610"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEV2128079.1"
                     /translation="MATTKFPDISQDRMGGDAAAARTDGARGGFPRGGSRDRSSVLDV
                     NNFDAIRIRLASPEAIRSWSHGEVVKPETINYRTLKPEHGGLFCERIFGPTKDWECYC
                     GKYKRIRFAGTVCDKCGVEVTRSKVRRERMGHIELAAPVAHIWYVKGTPSRLGLLLDL
                     PPRTLERVLYFASYMVTDVDDAKRQQAIDEINELHEETIAELRAEAQSKVDEILESSG
                     ADVSSIEDNAAQMQQRVTIERDAALAELEQEYNDTKAQIEDAMGAAAEKRWTFRRKVV
                     IKKGEAINADRLQKLDDVYGRLRDDIISEAEHHLSGGDMLAGAERDQLSHDATEQVKK
                     IEQELEEQVKAETADRDETIKQLTDLHERQVLTENDFRTLQEVVPPGVFEAGMGAEAV
                     YSYVSRKIDLDQLAVELRNEMQSASDVRRKKATKRLRVIEALRKSGNKPHWMIFTALP
                     VIPPELRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGAPEIIVRNEKRMLQE
                     AVDALIDNGRRGRAVSGSSKHKLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGP
                     DLALDECGLPKRMALELFKPFVMRRLVERDLAHNIKAAKRFVERVHPDVWDVLEDVIQ
                     DYLVLLNRAPTLHRLGIQAFRPKLIEGSAIQLHPMVTTAFNADFDGDQMAVHVPLSDA
                     AQQEARTRMLSVNNLLAPANGEPIVAPTQDIVLGIYYMTSLRDHAMDMERGTVPRGYG
                     KSFSSLEEVKLAYDSGILDVQAEITVRTDRDGGEMKRIQTTVGRALLNLELPEELGKF
                     YNETMGRKQLRGVIADCYRTFENPAETARIANDLKRLGFALATRGGVSIALADVTESE
                     TKQELVDEAQKAADAVDRQYRRGLVTEAERLRELEVIWNNARDTLTEDVEARLKGQNS
                     IYMMADSGAKGNMNQISQMAGMRGLVLDPGGKIIDIPIKSNFREGLTVLEYFLSTHGA
                     RKGLADTALRTADSGYLTRRLVDVAQDVIVTMEDCGTDKGIVAPRIEADGRELTADEY
                     RARIAGRIAAADVVHPETGEIIARPGDEISERAVDEDGNVRDLVQAVIDSGVEKINFR
                     SVMACQAPHGVCQLCYGRNLATGKLVEKGEAVGIIAAQSIGEPGTQLTMRTFHTGGVA
                     RADITTGLPRVEELFEAREPKGAALLAPRAGRVSIEDEDEERVLRIRSVEEHSWHEEL
                     GDGYEVIIQEGSQVQRGKTVIAHGPNGEELFAGVDGTFFLDGRTVYIRSESEEVDEFH
                     INYDDQILIEDGQEVTPGTQLTFGPKNPHALLATLGPGATQRYIIDEVQKVYRSQGVA
                     TNDKHIEVIARQMLRKVQVAFPGDTDLLEGDTVDRYSFSLQNEGALVQGGEPATAAPL
                     LLGITKASLETDSFLSAASFQETTRVLTEAAINGKVDHLRGLKENVIIGKLIPAGSGF
                     EHDQREPEPEALQEATVAAMRGEAAIDTEASYEELMQPEGGPVTAVMDREPDSEDGQR
                     APEDAAEINDADVEDETVQPQEQE"
     gene            complement(12283..15855)
                     /locus_tag="VGR22_05615"
     CDS             complement(12283..15855)
                     /locus_tag="VGR22_05615"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV2128080.1"
                     /translation="MAVESTSMTGAATHAVGAQQVISNIGIGRKSYADIQTVLEMPNL
                     VQIQLDSFNWFITKGLEELLKEVSPITDHHDKMQLHLSEPRFDEPWERLPEGEIRERA
                     KRDSRVAEVYCRERDMTYSARLRISAKLIINETGEVKETGPDGIFIGDFPVMTSDGTF
                     IINGAERVVVSQLVRSPGAYFERKPDPATGRLLSTAKLIPNRGAWLEFETSNRDVISV
                     KIDRKRKMPVTILLRALGYDTDDELLALFSDIDTSETRRFIQSTLEKEPTKSQGEALV
                     ELYRRMRPGDPPTRDNAERTLHDLFFNERRYDLARVGRHKLNQRLHQDDEVQTDSRTL
                     EKEDLVEIVRELIRLNNGQSHPDDIDHLGNRRIRAVGELIQMHVRTGFQRLERGIKER
                     MTIQDIETVTPASLISTTRPVIAAVREFFGGSQLSQFMDQTNPLAELTHKRRLSALGP
                     GGLSRDRAGMDVRDVHPSHYGRICPVETPEGPNIGLIGYLATYGKINDFGFIETPYRR
                     VVSSLVVDDPALPLEGQILRDNAFNSETDEVVVEAGTTLDAALIEKLRSSNVKQVRIR
                     PVVSGIIDYLSADREEDKLIAQADTPLDEVNRVDVDLVTVRRVAEGHTFPQVRPEQVE
                     YMDVSPKQVISVATALIPFLEHDDANRALMGANMQRQAVPLVRPQAPIIGTGVEHLAA
                     RDSGQVIVSQHSGTVTEASGSRIVVEDDQGEEHIYQLQKFVRSNQDTCINHRPTVHRG
                     QRVEPQQILADNSSTDNGELALGQNVTVAFMSWEGGNFEDAILLSERLVRDDVYTSIH
                     IEKYEAEARDTKLGPEEITRDIPNVGEDSLHDLDENGIVRIGAEVRPNDILVGKVTPR
                     GETELSAEERLLRAIFGEKAKEVKDTSLRVPHGVHGKVIDVKRFTREEEESSSHELPA
                     GVNESVRVMIAQKRKISEGDKMAGRHGNKGVISRILPMEDMPYLPDGTPVDIILNPIG
                     VPSRMNIGQILETHLGWAAKTLGFKVATPVFDGASEGEIREELIAAGLPEDGKVDLYD
                     GRTGEKFDRPVTVGVIYMLKLAHLVEDKIHARSTGPYSLVTQQPLGGKAQFGGQRFGE
                     MEVWALEAYGAAHTLQEMLTVKSDDIVGRVRTYESIVKGEPIGEAGVPESFKVLVKEL
                     RSLGLSIDVINEDEQTVDFTEDSTRDLLSNLDRINLSGFERTAD"
     gene            16508..17899
                     /locus_tag="VGR22_05620"
     CDS             16508..17899
                     /locus_tag="VGR22_05620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HEV2128081.1"
                     /translation="MKSRTLRIWLRPGIKIKRWIGLFMLAIVLLGLALAMFAVWIYRS
                     YDFPDSLQGPVGWLTLQPISQLWRAVLLLASALILLAFAFYRVSSSLLTPLLAASPGT
                     RPLSEIVNEHHFGPVKPELNVVAIGGGTGLSALLRGLKLHDIDITAIVTVADDGGSTG
                     RIRDVFNMPAPGDIRNCMVSLADDESLMGRLFHYRFDTEGSELSGHSFGNLFITAMTQ
                     VTGSFERAVIESSDVLNVRGRVLPSTVENVTLCAHMADGSVLRGESTIAHEKPAISEI
                     FLEPDAPGAYTPALAAILKADLVILGPGSLYTSVLPNLLVDGVREAIRWSTAPTAYVC
                     NVATQHGETDNYGYEDHVREVMRYLGKDQLNYALVNDNPAAAEAIRDEWQVEAILYDG
                     QTHVDDSVEIIARDMVSNSNPLRHDPAKLSEVLIEIARRAQAERLAQQREHRGTVLVA
                     PTTTRAEDQRFSA"
     gene            18044..19093
                     /gene="gap"
                     /locus_tag="VGR22_05625"
     CDS             18044..19093
                     /gene="gap"
                     /locus_tag="VGR22_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEV2128082.1"
                     /translation="MMYKVGINGFGRIGRQVFKAVQQGGFEDLFEVVAVNDLTDNDTL
                     AHLLKYDSNYGITEGEILAKEDGIDANGHFLKVYEERDPALLPWGELGVDLVIESTGR
                     FTSPDKARGHIEAGAKKVIISAPLSKTSESSTGATDAITVVLGVNEDAYNPGEHHIIS
                     NASCTTNCLAPIAKVLNDTFGVEYGMMTTVHSYTNDQVILDGPHKDLRRARSAGQNII
                     PTTTGAARAISLVIPALEGKLDGFALRVPTPTVSIVDLVATTTKPVSRESVNQAFKEA
                     SESEELLGILGYSEEPLVSMDYKQDARSSIVDADSTIVSGDRLVKAIAWYDNEWGYSC
                     RIADLTAVICEQGFA"
     gene            19182..>19462
                     /gene="pgk"
                     /locus_tag="VGR22_05630"
     CDS             19182..>19462
                     /gene="pgk"
                     /locus_tag="VGR22_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEV2128083.1"
                     /translation="MSKRSIRSANGAGKHVLVRVDFNVPIEDGRITDDTRIRAALPTI
                     IWLIDNGAKVILASHLGRPRGKVVDELRLVPVATHLSTLLDRPVAAMTS"
BASE COUNT         3308 a         6583 c         5996 g         3575 t
ORIGIN      
        1 agtcgcggaa cgtgcccgcc cccccggcca gcgccagcgt cttggtgatc gccgccgtca
       61 gcgtcgtctt gccgtgatcc acatgcccga tcgtccccac gttcacgtgc ggcttcgtcc
      121 gctcaaactt ctgcttggcc acgtgtactc tccctgggcg gtctacgccc actcattggt
      181 gccattcgtc aataaaaccg gctcgccgcg gagcgggccg cacggtgcgg cccgctcctg
      241 cctttcctag ctgcgcgcgg tagccttctc gacaaattcc tgtgccaggt tgtccggcag
      301 cggcgcgtag tgatcgaact ccatggagga aaccgcacgg ccctgcgtca tggagcgcag
      361 gtcggtgacg tagccgaaca tgttggccag cggcaccagc gcgcgaacga cctgggcgcc
      421 agcgcgctct tccatgccct ggatctggcc gcgacgagag ttcaggtcgc cgatcacgtc
      481 gcccatgaac tcttcgggcg tggtgacctc gaccgacatc accggctcga gaaccaccgg
      541 cttgccgcgg ttgagcgcct ccttgaacgc cagcgatccg gcgatcttga aggccatctc
      601 ggaggagtcg acctcgtgga aggagccgtc aacgagcctc gccttgacgt ctaccaccgg
      661 gaagccagcc ttgccgccgg tgtccatcgc ctcccggatg ccggcgttca cggcgggaat
      721 gtactcccgg gggatcgcgc caccgacgat agcatcctcg aactcgtagc cctcgccacg
      781 gtcacgcggc ccgaactcga caatgacatg gccgtactgg cccttaccgc cggactggcg
      841 gacgtgccgg ccctcgacac gcgcctcgcg cgtcagcgtc tcgcggtagg ccacctgcgg
      901 ccgaccaacg ttcgcgtcga cgcggaactc gcgcatcatc cggtctacga tgacatcgag
      961 gtgcagctcg cccatgccgt cgatgacggt ctggccggtc tcctcatccg tcttcacgcg
     1021 gaaggtcgga tcctcctccg cgaggcgctg cagcgcgttg gacatcttgt cctggtcggc
     1081 gcgggtcttc ggctcgatcg agaccgagat gaccggctcc gggaaggtga tcgactcgag
     1141 cacgatctgc tggtccgggt cgcacagggt gtcgcccgtc gtggtgtcct tgagaccgat
     1201 caccgccgcg atgttgcctg cggaaacctc cggaatctcc tcgcggtggt tcgagtgcat
     1261 ctgcagcagg cgtccgatgc gctcgcgccg gcccttcgtc gagttgagcg cgtaggagcc
     1321 ggactgcatg gtgcccgagt agacacgcac gtaggagagc ttgccgacat gcgggtcgga
     1381 cacgatcttg aaggcgaggg ccgagaacgg cgcgctcgga tccgcggtgc gcgtgatctc
     1441 ctcctcggtc ttcgggtcga tgcccttgac cggcgggaca tccagcggcg acggcaggta
     1501 gtcaatgatg ctgtcgagca tcggctgcac gcccttgttc ttgagggcgc tgccggccat
     1561 caccggcacc agcgtgctgt tcagcgtgcc ggcgcggaga ccggcgacca actcctccga
     1621 ggtgatcttc tcaccctcga ggtacttcat catcagctcc tcatccaact cggcaacctt
     1681 ctcgaccagc tcaacgcggg ccgcctcggc cgcttcctgg aactcggccg ggatctcgcc
     1741 gacctcgatg ttctgaccga ggtcgtcgtg gtaggtgatc gccttcatgt tgatgaggtc
     1801 gatcacgccg acgaacgtat cctcggcgcc catcggcagc tggaccggag cggggttcgc
     1861 cttgaggcga tccacgatca tctcgaccgt gcgctcgaag ttggcgccgg tgcggtccat
     1921 cttgttgacg aagcagatgc gcggcacgtt gtacttgtcc gcctgccgcc agactgtctc
     1981 ggactgcggc tcgacgccgg ccacgccgtc gaacaccacc acgccaccgt cgagcacgcg
     2041 caacgagcgc tccacctcga cagtgaagtc gacgtggccc ggggtgtcga tgatgttgat
     2101 acggtgatcg cgccagaagc aggtcgtggc cgccgaggtg atcgtgatgc cacgctcacg
     2161 ctcctgagcc atccagtcca tcaccgcagc accctcgtgg gtctcgccaa ccttgtggac
     2221 gatacccgtg tagaacagga tgcgctccgt cgtcgtggtc ttgcccgcgt caatatgcgc
     2281 gatgatgccg atgtttcggg tgcggcgcag cgcgacctgg ggatcggcaa ggtttggttg
     2341 ggtcgcagta ccagccattg ttgggcctaa atcctctctt gcacgtcggt gtgctcgaac
     2401 acattggaga caagccgttc tcggcgtcgc tccagatccg ccctagaagc ggaagtggga
     2461 gaacgcgcgg ttcgcctcgg ccatccggtg cgtatcctca cgcctcttga tcgtggcacc
     2521 ggtgccgttg taggcatcga ggagctcgtt cgccagcttc tcctgcatgg tcttgccggc
     2581 tcgggctcgc gccgacgcca gcagccagcg gatcgccagc gactgtcgtc gctgcccttc
     2641 gatctgcacc ggcacctggt aggtcgcgcc accaacgcgg cgcggcttca cctcgagcat
     2701 cggcgtggca ttctgcactg cctgctcgaa tacggtgatg gggtcgtttc cagtgcgatc
     2761 ctggatcatc tgcagcgcgt cgtacatgat acgctccgcc agacccttct tgccccgctg
     2821 catgatcttg ttgatgaagc gggtcaccaa ctcgctgttg taacgagcat caggctgtgg
     2881 cacacgacgc tcgatcttgg ctcgtctcgg catgggttct ctgtcgcctc cgtgctgtcc
     2941 atagcggggc ccggattaac cgaatccggc acccttgtcg gacatcctgg ctggtccgcg
     3001 gcccgatgcc gccccagcct ctaccactca cccggggcat gtcatcatcc acccggatca
     3061 ctcagcccgc caccaggcgg gtgcttgtcc gggccgtcgc ccgggccatc gcgaaaggtc
     3121 gaaatcctcg ttcgcaagaa ccgaaggaac gctggaggcc aagacacacg aaaaagcggt
     3181 cgccgccctt tggatcagga cgtcgtgccg ctctccgcat ccagcaccct cagttccgcc
     3241 agtggcggaa acaccgaacc ggcccgtcag ggccgcggga tcactcccta gctgctcggt
     3301 gccttggttc catacttgga ccgcgcctgc ttgcggtttt caacgccgcg agcatccaga
     3361 gcacctcgaa cgatgtggta ccgcacaccc gggaggtcct tcacacgacc gccacggata
     3421 agcaccaccg agtgctcctg aaggttatgc ccctcgcccg gaatgtatgc ggtcacttcc
     3481 acaccattcg ataggcgaac acgggcgatc ttgcgcagtg ccgaattggg cttcttgggc
     3541 gtcatcgtct tgacctgagt gcacaccccg cgcttctgcg gcgcgaaggt gtcgccacgc
     3601 agggttcccg catagcgacc cgccgagctg ttggtgaagc gcagcgcggg cgacttggtc
     3661 ttcttcttga cgctctttcg gcccttgcga accaactgat tgatcgtcgg cacgaaaagc
     3721 tcctagccag acagggggct cacctaaacc gcgctttgcg cacaaaaaac cggtattcgc
     3781 aaccggtttg gcgcagctgc ggcgcctcct gcctcgtcag ttgacggcaa tcgtgaacca
     3841 gttaccggtc ctttgatcgc gcacgtatct ggcgatcccc caaggagatc acaaccgtga
     3901 ataatagggg tttcgggaga ccgcgtcaag gcacgacggt tcgtgcggcc agtgccgcga
     3961 accctcgctg cttgatcggc ttggccgcac gccaccggga gcgacggcgc aacacccgct
     4021 ccgcacgcgc gacctccgtc gaccgaccgt gcaggatggt gtgtacgtat gctgcaccac
     4081 atcgctccac cgcgtcaaca ccccgctcga tctcgtctgt gctcgcctgc acgggcagcg
     4141 ccaggatcgc gacatcgacc ggcacgtcga gattcagcgc ctcgacgccc cacactggca
     4201 ttgcctggac cgctgtcccc gccaccgcgc gtgtcgtcga gcgcagcgca agcacgtccc
     4261 gcccggcgat caccccatgt gccccaggcc agagcagcga tgcagcgacc gccaggtgcc
     4321 cgacccccgg atcgacgatc aaccatcgcg ccggtggtac gcccgctggc agatctcgca
     4381 tcaactccgc cgcgagcttg gtcgcgtgcc ccagttcatc gtattcgggc aagccgatca
     4441 ccggaatcag gtgccactcc aggtctgccg ccacaaacct cgcctcgggt tgatcgaaca
     4501 gtccctcggc aaacgggtca cgctcggtca ccttgccggc aacccgccgc aggtggacca
     4561 cggtctgctc ggtgcccggc tcgaccaact cgaccgtcgt gtctcctccc gtgaactcct
     4621 cggcgaagag gtcggcgagc ggatggacca ccacgacagc caacagccct cctggggcca
     4681 ccgtaccgag cgccacgtcc agcaacccgg cgattaccgg tcgccccgcc ttgcccggca
     4741 cgttccacag tacgaggtcg tatccgctat ctgtcgctgg gagcgaaaag tcgatgttga
     4801 atccagtcac cgcgtcgccc agttgccgcg cgttgtgcgc gctgaacgtg acggcaagtg
     4861 cgtcccgatc gacgtagcgc atcgaccagc cgggcttact cgattgccac gcgataccca
     4921 gcacgccgta gccacagccg aaatccgccg ccgacccggt ctccggaaac agcgtgggat
     4981 cgaggctgtc gagcagcaca tggctgccct tgtcgatctg ctggctgctg aacagatcct
     5041 gggcgacatc gaaccgcagc tcctgcccct gccactggag cacaacggcc ttctttattg
     5101 gtaggggcaa accagtcacg gtgatcctca tgccgacggc gagcggttgc ggtgccgtcc
     5161 cgacaccggc taccatcgcg cagatcggtc gggtccatgc cgtcatcact gaggagtcta
     5221 tcgtgtcgaa atcgttgggt attgggatcg ccggcgctgg cttcatcggt aaggtgcaag
     5281 cgaacgcgct gcgggagatc gacggtgccg aggtggtcgc catcgccgac ccgcgcgaag
     5341 acgccgggca ggcgctcgcg acggcaactg gtgcccgctg gtatccctcc tacactgaga
     5401 tgctggagca tccagagctc gatgtagtgg tcctgaccac gccgtccgga ctgcatgccg
     5461 aacaggccgt actcgccgcg caggcgggaa agcacatcat caccgagaag ccgatgagca
     5521 ccacgccgca tggcatccaa ctcatgactg aggcggcgga gcgggccaac gtcgagctgg
     5581 cagtgatctt ccagaaccgt ttttcccgtg acgtgatcaa ggtcaagcgc ggcatcgagg
     5641 cgggcatgct cgggcgcccg gttatgggca gcgccgcagt ccactggcac cgtacagcgg
     5701 agtactacga ggccaacgac gggtggcgcg gcacgtggac cctcgacggc ggcggagcgt
     5761 tgatgaacca aagcgtccac accatcgatc tgctgcagtg gctgatgggc ggcgtgagcg
     5821 ccgtttcggc gaatgccgcc accctcatcc acgagatcga gacggaagac accgccacgg
     5881 cgagtgttgt attcaggtcc ggtgcgctcg gcacgattgc cgggacgacc agcgccagca
     5941 ccgacgcgcc ggtgcgtgtt gagatcgtcg gcaccggggg aagggccatg ctcgagcgcg
     6001 gtgtgttggt ggcgtgggag ggcgactcgc cgctgaccga cgacatcctg ctgccgttcg
     6061 accacgacta catacagggg tgggagccag gcgagccgtt cggtgcggcc cacaagcgcc
     6121 agcttgcagt gatcctcgat gccttgcgct ccggtgacac gccgccggtc ccaccccggg
     6181 aagcgcgcaa ggcagtcgac atcgtcctcg ccgtctatga gtcggcgcag accggcaagc
     6241 acgtcgcgat cgattagtca gcctgcgcgc cgcaatttgc cgatgaatgc acaaagcagt
     6301 gcgtcatttc gagcggagcg gcgcagccgg ggagtcaaga aaactcctgc ccgccgggag
     6361 tcgactcctg tgacacaagc tttctccccg cgcacccttc gagtggtctg atcgaaatga
     6421 catcgattgg atagtcctca aggcaggtgc ggacacagtc tgcgtatcgc tcgctcgcgt
     6481 gagaggctcc cagctcccat ggaatacgcc atcttcaccg tctccacccc gcagttttcg
     6541 ccagaagttg tggtccatcg caccaaggcc gccgggtacg acggcatcga atggcgcgtg
     6601 aaggacgacg cacccagcga cgctcccacc ttctggagcg gaaaccgggc cacgctgccg
     6661 ctcaccggtc tcgaagagaa cgcgccgacg tggcggcgga tggccgagga cgtcgggctg
     6721 gagatgccgg cgatcgccag ctacgtgaag tgcgacgagc tcgagcgcgt ggagctggcc
     6781 atgcggggag cggccgcgct cggtgtgccg atgttgcgca tccagctacc aacctaagac
     6841 ggctctgagg cgttcacccc gctcagagat gccgcgcggg aacagtacgc gactgttgcc
     6901 gatctcgccg cccggtatgg cgtcaaggcg ctggttgagc tccaccaccg gacggtcgtc
     6961 ccgtcggcca gcgccgcccg ccggttcctc gatgggctgg acccggcgca catcggcgcg
     7021 atctacgatg ccggcaacat ggtgatcgag gggtgggagc atccacgcat ggcactggag
     7081 gtgctcgggc cgtacctcgc gcacgtccat ttcaagaatg cgcgatggac acccgcggag
     7141 acgctgtcag acggcacggt gcgctgggag catggattct gtccctttga ggaggggatc
     7201 gccgacctgc gggaagtgat gcgggcgctc cagcaggtcg ggtacgatgg ctggatcacc
     7261 ttcgaggact tcagcactga ccgcccggtc gacgaactgc tcacccgcaa cctcgcgtac
     7321 gtaaaggcca tcgagcggga ggtcgtgacc gaagacgtcc aacgataatt ggtgtcaatc
     7381 agagccgacg agtcgcgcac ggcccgaagc cgtgacgggc ctgcagcctg ccacgcggca
     7441 gggtcggcta cggcgggttg caatgcactc ggaatagggg ccgacctccc gcagggagtg
     7501 gtcggcccgc tcgaccaaga ggaacccgcg catctcagag cgggtctaca tcaagaagcg
     7561 cccagacaga gatcatcctg gaaaccacaa caacgacccg gccggtttcc actggccggg
     7621 tcgttgtctt gtgcgtcatc cgaagtccgg agcggtctac tcctgctcct gcggctggac
     7681 ggtctcgtcc tcgacgtccg cgtcgttgat ctccgcggcg tcctccggag cgcgctggcc
     7741 gtcctcggag tccggctcgc gatccatcac cgcagtcacc ggtccaccct cgggctgcat
     7801 gagctcctcg tagctcgcct cggtatcaat ggcggcctca ccgcgcattg ccgcgaccgt
     7861 tgcctcctgg agcgcttccg gttctggctc gcgctgatcg tgctcgaagc cggagcccgc
     7921 cgggatgagc ttgccgatga tcacgttctc cttcaggccg cgcaggtggt cgaccttgcc
     7981 gttgatcgcg gcctcggtga gcacccgggt ggtctcctgg aaggatgctg ccgagaggaa
     8041 cgaatcagtt tcgagcgacg ccttcgtgat gcccagcagc agcggggccg ccgttgccgg
     8101 ctcgcctccc tgcaccagcg caccctcgtt ctgaaggctg aaggagtacc ggtcgaccgt
     8161 gtcaccctca agcaggtcgg tgtcgcccgg gaaggctacc tgtaccttgc ggagcatctg
     8221 ccgcgcgatt acctcgatgt gcttgtcgtt ggtcgccacg ccctgcgagc ggtagacctt
     8281 ctgcacctcg tcgatgatgt agcgctgcgt cgcacccggg ccaagggtcg ccagcagcgc
     8341 gtgcgggttc ttcggcccga acgtcagctg cgtgcccggc gtgacctcct ggccgtcctc
     8401 gatcaggatc tggtcgtcgt agttgatgtg gaactcgtcc acctcttccg actcagatcg
     8461 gatgtagacc gttcggccgt cgaggaagaa ggtgccgtcc acccccgcga acagctcctc
     8521 tccgttcggt ccgtgcgcga tcaccgtctt gccacgctgg acctggctgc cctcctggat
     8581 gatgacctcg tagccgtcgc caagctcctc gtgccagctg tgctcctcga ccgagcggat
     8641 ccggagcacg cgctcctcgt cctcatcctc gatcgacacc ctgccggccc gcggtgccag
     8701 cagcgcggcg cccttcggct cacgcgcctc gaacagctcc tcgacacgcg gcagaccggt
     8761 cgtgatgtcg gcccgcgcca cgccaccagt gtggaaagtg cgcatcgtca gctgcgtgcc
     8821 cggctcgccg atcgactgtg cggcaatgat gccgacggcc tcaccctttt cgacgagctt
     8881 gcccgtcgcc agattacggc catagcagag ctggcaaacg ccgtgcggag cctggcaggc
     8941 catcaccgag cggaaattga tcttctccac gccggagtca atcaccgcct gtaccaggtc
     9001 acggacgttg ccatcctcgt ccacagcgcg ctcactgatc tcgtcgcctg ggcgggcgat
     9061 gatttcaccc gtctccgggt gcacgacgtc ggcagccgca atgcggcctg cgatgcgcgc
     9121 ccggtactca tcggcggtga gctcacgccc atcggcctcg atccgggggg caacgatgcc
     9181 cttgtccgtg ccgcagtcct ccatcgtgac gatcacgtcc tgtgcgacgt ccacgaggcg
     9241 acgcgtcagg taaccggagt ccgcggtgcg aagcgccgtg tcggccagac ccttgcgggc
     9301 gccgtgggtc gagaggaagt actcgagcac ggtcaggccc tcacggaagt tggacttgat
     9361 cgggatgtcg atgatcttcc cacctggatc aagcaccaga ccgcgcatgc cggccatctg
     9421 gctgatctgg ttcatgtttc ccttcgcgcc ggagtccgcc atcatgtaga tcgagttctg
     9481 gcccttgagg cgcgcctcga catcctccgt cagggtgtcg cgggcgttgt tccagatcac
     9541 ctccagctcg cggagccgct cggcctcggt gaccagacca cgacggtact gacggtcaac
     9601 cgcgtcggcc gccttctgcg cctcatcgac gagctcctgc ttcgtctccg actccgtcac
     9661 gtcggccagt gcgatgctga caccgccgcg agttgccagc gcaaagccca gccgcttgag
     9721 gtcgttcgcg atccgcgccg tctcggccgg gttctcgaac gttcggtagc agtccgcgat
     9781 cacgccgcga agctgcttgc ggcccatcgt ttcgttgtag aacttgccga gctcctcggg
     9841 cagttccagg ttgaggagcg cacgcccgac cgtggtctgg atacgcttca tctcgccgcc
     9901 atcacggtcc gtgcggacgg tgatctccgc ctgcacgtcc aggatgcccg agtcataggc
     9961 cagcttgacc tcctccagtg aggagaagct cttgccgtac ccacgcggca ccgtgcctcg
    10021 ctccatgtcc atcgcatgat cacggagact ggtcatgtag tagatgccga gaacgatgtc
    10081 ctgcgtcggc gccacgatcg gctcaccgtt cgccggcgcc agcaggttgt tcacggacag
    10141 catacgcgtc cgcgcctcct gctgcgcagc gtccgagagc ggcacgtgca cggccatctg
    10201 gtcgccgtca aaatcggcgt tgaacgccgt ggtgaccatc gggtgcagct gaatggccga
    10261 cccctcgatc agcttcggac ggaacgcctg gatgccgagc cggtggagcg tcggggcgcg
    10321 gttgaggagc accaagtaat cctggatgac gtcctcaagc acgtcccaga cgtccgggtg
    10381 gacccgctcg acgaaacgct tcgcggcctt gatgttgtgg gcgagatcac gctcgaccag
    10441 gcgccgcatc acgaacggct tgaagagctc cagcgccatc cgcttcggca ggccacactc
    10501 atccagcgcg agatctggac cgaccacgat caccgagcga cccgagtagt ccacgcgctt
    10561 gccgagcagg ttctggcgga atcggccctg cttgcccttg agcatgtcgc tcaggctctt
    10621 gagcttgtgc ttactcgagc cggagacagc acggccacgc cgaccgttgt caatcagcgc
    10681 gtcgacagcc tcctgcagca tgcgcttctc gttgcggaca atgatctccg gcgccccgag
    10741 ctcaagcagg cgcttgagac ggttgttgcg attgatgaca cggcgataga gatcgttcag
    10801 gtccgaggtc gcaaagcgac caccgtcgag ctgcaccatc gggcgcagtt cgggcgggat
    10861 caccggcaac gccgtgaaaa tcatccagtg cggcttgttg ccgctcttgc gcagcgcctc
    10921 gatcacgcgc agccgcttgg tcgccttctt ccgacgaaca tccgacgcgg actgcatctc
    10981 gttgcgaagc tcgaccgcga gctgatcgag gtcgatcttg cgcgagacgt agctgtagac
    11041 cgcctcggca cccatgcctg cctcgaagac gcccggaggc acgacctcct gcagggtgcg
    11101 gaaatcgttt tcggtgagca cctgacgctc gtgcagatca gtaagctgct tgatcgtctc
    11161 gtcgcgatcg gcagtctcgg ccttgacctg ctcctcgagc tcctgctcga tcttcttcac
    11221 ctgctcggtg gcatcgtgcg agagctggtc acgctcggcg ccagccagca tgtcgccgcc
    11281 agagaggtga tgctccgcct cgctgatgat gtcatcacgg agcctgccgt agacatcgtc
    11341 gagcttctgc aggcggtcgg cgttgatcgc ctcgcccttc ttgatgacca ccttgcgacg
    11401 gaaggtccat cgcttctcgg cggcggcgcc catcgcatct tcaatctgcg ccttggtgtc
    11461 gttgtactcc tgctcgagct cggccagcgc ggcgtcacgc tcgatggtga cgcgctgctg
    11521 catctgcgcg gcgttgtcct cgatgctgct gacatcggcg ccgctgctct caaggatctc
    11581 gtcgaccttc gactgcgcct cggcgcggag ctcggcgatg gtctcctcgt ggagctcgtt
    11641 gatctcgtcg atcgcctgct gccgcttggc gtcatcgaca tccgtcacca tgtaggaagc
    11701 gaagtagagc acccgctcca acgtgcgcgg tggcagatcg agcagcaggc cgaggcggct
    11761 cggcgtgccc ttgacatacc agatgtgcgc aaccggcgca gctagctcga tgtggcccat
    11821 gcgctcgcgc cgtaccttgg agcgcgtcac ctcgacgcca cacttgtcac agacggtgcc
    11881 agcgaagcgg atgcgcttgt acttgccgca gtagcactcc cagtccttgg ttgggccaaa
    11941 gatgcgctcg cagaagagac caccatgctc gggcttgagc gtgcgatagt tgatcgtctc
    12001 cggcttgacg acctcgccgt gcgaccagct gcggatcgcc tcgggcgacg ccagccggat
    12061 gcggatcgcg tcgaagttgt tcacgtcgag aacggagctc ctgtcgcggc tgccgccacg
    12121 aggaaaccct ccacgcgcac cgtcggtccg agcggctgcg gcgtctccac ccatcctgtc
    12181 ctgcgaaata tcagggaact ttgttgttgc catggttcct ccatcgatcg ccaaggaaac
    12241 cggcacgggg cgggcgcgcc gggacacgcc cgccgcgccg cgctagtccg ccgtgcgctc
    12301 gaagccactc aggttgatgc gatcaagatt ggagagcaga tcgcgggtcg aatcctccgt
    12361 gaagtcgacg gtctgctcgt cctcgttgat cacgtcgatg gagaggccga gactgcggag
    12421 ctccttgacg agcaccttga acgattccgg cacgccggcc tcgccgatcg gctcgccctt
    12481 gacgatcgac tcgtaggtgc gaacgcgacc cacaatgtcg tccgacttga ccgtcagcat
    12541 ctcctgcaat gtgtgcgccg cgccgtaggc ctctagcgcc cacacctcca tctcgccgaa
    12601 tcgctggcca ccgaactgcg ccttgccgcc cagcggctgc tgagtgacga gcgagtacgg
    12661 acccgtggac cgtgcgtgga tcttgtcctc caccaagtgg gcgagcttga gcatgtagat
    12721 cacgcccact gtcaccgggc ggtcgaactt ctcacccgtg cggccgtcgt acagatcgac
    12781 cttgccgtcc tccggcagtc cagccgcaat gagctcctca cgaatctcac cctccgaggc
    12841 gccgtcgaag accggcgtcg ccaccttgaa gccgagcgtc ttggccgccc agccgaggtg
    12901 cgtctcgagg atctgcccga tgttcatgcg cgacggcacg ccgatcgggt tcaggatgat
    12961 atcgaccgga gtgccatccg gcaggtacgg catgtcctcc atcggcagga tgcgactgat
    13021 cacacctttg ttgccgtggc ggccggccat cttgtcgccc tcggagatct ttcgcttctg
    13081 cgcgatcatc acgcggacgc tctcgttcac cccggcggga agctcgtgac tgctgctctc
    13141 ctcttcctca cgggtgaagc gcttgacatc gatcaccttg ccgtggacac cgtgcggaac
    13201 acgcagggag gtgtccttta cctccttggc cttctcaccg aagatcgccc ggagcagccg
    13261 ctcctcggcg ctcagctcgg tctcgccacg cggcgtgacc ttgccgacga ggatgtcgtt
    13321 cgggcgaacc tcggcgccga tgcgcacgat gccgttctcg tccaggtcgt gcaggctgtc
    13381 ctcaccgacg ttcgggatat cccgggtgat ctcttcgggt ccgagcttgg tgtcgcgcgc
    13441 ctccgcctcg tacttctcaa tatggatcga ggtgtagacg tcgtcgcgga ccagccgctc
    13501 ggagagcagg atggcgtcct cgaagttgcc accctcccaa gacatgaagg ccacggtcac
    13561 gttctggccg agcgccagct cgccgttgtc ggtcgaggag ttgtccgcca ggatctgctg
    13621 cggctccaca cgctggccgc gatgcaccgt cgggcggtgg ttgatacaag tatcctggtt
    13681 cgagcggacg aacttctgaa gctgatagat gtgctcttcg ccctggtcgt cctcgaccac
    13741 gatgcggctg ccgctcgcct cggtcaccgt accggagtgc tgcgagacga tcacctggcc
    13801 ggagtcgcgc gctgcgagat gctcgacacc cgtgccgatg atcggcgcct gcggccgcac
    13861 cagcggcact gcctggcgct gcatgttcgc gcccatgagc gcgcgattcg cgtcgtcgtg
    13921 ctctaggaac gggatgagcg ccgtcgccac cgaaatcacc tgcttcgggg agacgtccat
    13981 gtactcgacc tgctccggcc gaacctgggg gaaggtgtgc ccctcggcga cgcgacggac
    14041 ggtcaccaaa tccacatcga cccgattgac ctcgtcgagt ggcgtatccg cctgcgcgat
    14101 gagcttgtcc tcctcgcgat cagccgagag gtagtcgatg atgccggaga ccaccggccg
    14161 gatacgcacc tgcttcacat tggaggaacg cagcttctcg atcagcgcag cgtcgagcgt
    14221 cgtgcctgcc tcaacgacga cctcatcggt ctcgctgttg aaggcgttgt cacggagaat
    14281 ctggccctcc agcggcagtg ccgggtcgtc caccaccaag ctggaaacca cccggcgata
    14341 cggtgtctcg atgaagccga aatcgttgat cttgccgtag gtcgccaggt agccgatcaa
    14401 gccaatgttc ggaccttccg gcgtctccac ggggcagata cgaccgtagt gcgacgggtg
    14461 aacgtcacgc acgtccatgc cggcgcggtc acggctcagg ccacctgggc ccagcgccga
    14521 aagacgccgc ttgtgcgtca actcggccag cgggttggtc tggtccatga actgcgagag
    14581 ctggctgcca ccaaagaact cgcgcaccgc ggcaatcacc ggccgagtag tgctgatcag
    14641 gctcgccggc gtgacggtct cgatgtcctg gatcgtcatg cgctccttga tgccccgctc
    14701 gaggcgctgg aatccggtgc gcacatgcat ctggatcagc tcgccaaccg ctcggatgcg
    14761 gcgattgccg aggtggtcga tgtcgtccgg gtggctctgg ccattgttga gtcggatgag
    14821 ctcgcggacg atctcgacga ggtcctcctt ctccagagtg cggctgtcgg tctgcacctc
    14881 gtcgtcctgg tgcaatcgct ggttcagctt gtgccggccg acccgcgcca gatcgtagcg
    14941 ccgctcattg aagaagaggt cgtgcagcgt gcgctcggcg ttgtcgcgcg ttggggggtc
    15001 tcccgggcgc atgcggcggt agagttcgac cagcgcctcg ccctgcgact tggtgggctc
    15061 cttctccagt gtcgattgga tgaaccgacg ggtctcgctt gtgtcgatgt cgctgaagag
    15121 cgccagcaac tcgtcatccg tgtcgtagcc gagcgcgcgc agcaggatcg tcaccggcat
    15181 cttgcgcttg cggtcgatct tgacggagat gacgtcgcga ttggaggtct cgaactcgag
    15241 ccacgcaccg cggttcggaa tcagcttggc ggtcgacagc aggcgaccgg tggccgggtc
    15301 cggcttgcgc tcgaagtagg cgcccggcga acggacgagc tgggagacga ccacacgctc
    15361 ggccccgttg atgatgaagg tgccgtcgga ggtcatcacc gggaaatcgc cgatgaagat
    15421 accgtccggg ccggtctcct tgacctcgcc ggtttcgttg ataatcagct tggccgagat
    15481 gcgcagccgc gccgaatagg tcatatcgcg ctcacggcag tagacctcgg cgacacgcga
    15541 gtcccgcttg gcacgctcac ggatctcgcc ctccggaagc cgctcccagg gctcgtcgaa
    15601 acgcggctcc gagaggtgga gctgcatctt gtcgtgatgg tcggtgatcg gcgagacttc
    15661 cttgagaagc tcctcaagcc ccttcgtgat gaaccaatta aacgaatcca gctggatctg
    15721 gaccagattc ggcatctcca gcacagtttg gatgtcggcg taggacttcc gcccgattcc
    15781 gatgttggag atcacctgct gggcacccac agcgtgggtc gccgccccgg tcatcgacgt
    15841 actctctaca gccatgcaca gctcctcagt atgttgccga aaccgcagcg ggcttcggcg
    15901 cccggaactc cagcctcgat ctcacgcgca ctcagcccag tcgctcctgt tcttcgtgag
    15961 tctgaaataa gacatcatgt tgggaaatgg gggatcggcc tcgacgtcgt cacagctggt
    16021 gtgggacggc aggtaacaat accggcgacc gcctcgggcc gggggcgaac ctccgtggat
    16081 cagcgggcac gccaaagcag gagggcacag cgttcccctc ccggatcacc agggtgatgc
    16141 cttttcttgt gatgaacctg cacgcgctgt ccagcccaac caacgtgacg acgttgcgta
    16201 tcgatcacga gtatcacatg gccatcgacc atgcgaacga tcatgatacc ctgccatggc
    16261 gctcatgtca agcacccggg ccagcatcgc ccaatacgtg gtgttgccgg ttcgtcccgc
    16321 caatccggcg gtggccgaat cctacccgat tccggtgcta tactcaaaca ccgcgacagg
    16381 aaccgcgtcg atgacgagag ggtatccttg gcctcgggca cgcgcgacac gtggcctgac
    16441 tggaatcgtt ccgccaccag ctttcgctgt acgccgatcg ccggaaaaca catccgatgg
    16501 acagaccatg aaatcgcgaa ccctgcggat ttggctccgg ccaggcatca agatcaagcg
    16561 atggatcggg ctcttcatgc tcgccatcgt cctccttggg ctcgccctgg cgatgttcgc
    16621 cgtctggatc taccggagct acgactttcc ggacagtctg cagggccccg tcggctggct
    16681 caccctccaa ccgatttcgc agctgtggcg agccgtgctg cttctcgcca gcgcgctcat
    16741 cctgctcgcc ttcgccttct accgcgtcag cagctccctg ctcacgccgc tccttgccgc
    16801 ttccccgggc actcgaccgc tctcggagat cgtcaacgag caccatttcg gcccagtcaa
    16861 gccagagctg aacgtggtgg ccatcggtgg cggcaccgga ttgagcgccc tgctccgcgg
    16921 tctcaagctg cacgatatcg acatcaccgc catcgtcacc gtcgccgacg atggcggcag
    16981 caccggccgc attcgcgatg tcttcaacat gcccgcgccg ggcgatatcc ggaactgcat
    17041 ggtctctctt gccgacgacg agtcgctgat gggtcgcctc ttccactatc ggttcgatac
    17101 ggaagggtcc gaactctccg gccactcctt tggcaacctc ttcattaccg ccatgaccca
    17161 agtgaccggc agcttcgagc gcgccgtcat cgagtcctcc gacgtcctca acgtgcgcgg
    17221 tcgcgtgctg ccctccacgg tggagaacgt cacgctctgc gcccacatgg ctgacggcag
    17281 cgtgctcagg ggcgaatcga cgatcgccca cgagaagccg gccatctccg agatctttct
    17341 ggagccggat gccccgggag cctacacccc ggcactcgcc gcgatcctga aggccgacct
    17401 cgtcatcctc ggcccgggca gcctctacac cagcgtgctg cccaacctgc tggtcgatgg
    17461 tgttcgcgag gcgatccgct ggtcgaccgc ccccaccgcc tatgtctgca atgtcgccac
    17521 gcagcacggc gagaccgaca actacggcta tgaggaccat gtgcgcgagg tcatgaggta
    17581 ccttggcaag gaccagctca actacgcgct cgtcaacgac aacccggccg ccgccgaggc
    17641 cattcgtgac gagtggcagg tcgaggcgat cctctacgac ggccagacgc acgtcgacga
    17701 ctccgtcgag atcatcgccc gggacatggt cagcaacagc aacccgcttc ggcacgatcc
    17761 agccaagctc tctgaggtgc tgatcgagat tgcgcggcgg gcacaggccg agcgcctggc
    17821 gcagcagcgc gaacaccgag ggaccgtgct tgtcgcgccg accaccactc gcgccgagga
    17881 ccagcgattc tcggcatgat ccatgcttgc ctcttggcgg gtggacgaac tgttacgatt
    17941 ctcacaatta ctcgtccact agggtcgatc gaccgcggca gcgtctgggc acgctacacg
    18001 gcggtgtgcg gtcctcgctt cgagagtgaa gggaacaggg ctcatgatgt acaaggttgg
    18061 aatcaacggt ttcggccgca tcgggcggca ggtcttcaag gcggtccagc agggcgggtt
    18121 cgaggatctg ttcgaagtcg ttgcggtcaa cgacctcacc gataacgaca cccttgccca
    18181 tctcctgaag tacgactcca actacggcat caccgaggga gagatcctcg cgaaggaaga
    18241 tggtatcgat gccaacggcc acttcctgaa ggtgtacgag gaacgcgacc ctgccctcct
    18301 gccatggggc gagctcggcg tggacctcgt gatcgaatcg accggccgct tcacgtcacc
    18361 cgacaaggcc cgggggcaca ttgaggcggg cgcgaaaaag gtcatcatct ctgctccgct
    18421 cagcaagacc tccgagagct cgaccggggc gactgacgcc atcactgtcg tcctaggcgt
    18481 caatgaggat gcctacaacc cgggcgagca ccacatcatc tccaacgcgt cctgcacgac
    18541 gaactgtctc gcgccgatcg ccaaggtgct aaacgacaca ttcggcgtcg agtacggtat
    18601 gatgactaca gtgcattcct ataccaatga tcaggtcatc ctcgacggcc cacacaagga
    18661 tctccgtcgg gcgcgctcgg ccgggcagaa catcatcccc acgaccactg gcgccgcccg
    18721 ggcgatctcg ctcgtcatcc ccgccctcga gggcaagctc gacggcttcg cgctgcgtgt
    18781 cccgaccccg acggtctcca tcgtcgatct cgttgccacc accaccaagc ccgtaagccg
    18841 ggagtcagtc aatcaggcct tcaaggaggc aagcgagagc gaggagctgc tcggcatcct
    18901 cggctactcc gaggaaccgc tcgtctccat ggactataag caggacgcgc gctcctccat
    18961 cgtcgacgcc gattcgacga tagtttccgg tgatcggctg gtgaaggcca tcgcctggta
    19021 cgacaacgag tggggctact cctgccgcat cgcggacttg accgcggtca tctgtgagca
    19081 gggcttcgcg taggggaggg ccgtgtgccc gcccgcacgc ttgccacggg cagcgacggg
    19141 cccctcgctg cccggttgcc tctccagtgg gggaaagaag catgagcaaa cgctccatcc
    19201 gctcggccaa cggcgccggc aagcacgtgc tggtgcgcgt cgacttcaac gtaccgatcg
    19261 aggacgggcg catcaccgat gacacccgta tccgcgcggc gctcccgacc atcatctggc
    19321 tgatcgacaa cggggcgaag gtgatcctcg catcgcacct cggtcgcccc aggggcaagg
    19381 tcgtggacga gctgcggttg gtgccggtcg ccacgcacct ctcgacgctc ctcgaccgcc
    19441 ccgtggcggc gatgacctcg at
//
LOCUS       DASYWL010000158         6737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_363711, whole genome shotgun sequence.
ACCESSION   DASYWL010000158 DASYWL010000000
VERSION     DASYWL010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6737
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363711"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..607
                     /locus_tag="VGR22_05635"
     CDS             95..607
                     /locus_tag="VGR22_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128084.1"
                     /translation="MTISRGRPLTILFFLLATAIGVAIVPLAPTPGADDPMDTDFVDY
                     MQPRLSVLLDSAREVEGMVSERSRNLLALRAESARINALLEQIDTYLAAREPSPEEQL
                     VADLYRAGADNLRLAIDTASDALSRFDFSAIPEMIPVFTAGTELIEESLATMGTEQTR
                     RSSYTGTSVV"
     gene            700..906
                     /locus_tag="VGR22_05640"
     CDS             700..906
                     /locus_tag="VGR22_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128085.1"
                     /translation="MSTYTVRYQIGGDEHTDQLEAENAATAARLVEERHLEDDWFELI
                     EVHLVEEGESDTNPENSHSADQST"
     gene            965..3121
                     /gene="glgX"
                     /locus_tag="VGR22_05645"
     CDS             965..3121
                     /gene="glgX"
                     /locus_tag="VGR22_05645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEV2128086.1"
                     /translation="MSLPEVFGTPRPGRPIPLGAHWDGEGTNFAIYSRHASAVTLCLY
                     ENDNHEKPFATVPLDERMQDVWHVYLPGVHAGTRYGYSFEGEYEPAEGHRFNPNKVML
                     DPYAKALAGTVQWDPAVFPYQLGAEDDDVMDERPNDAFVPKAVVTGGDFRWEGDPRPN
                     IPWNESVIYELHVKGFTECNPAIPERLRGTWQGVSHEASIEYLKRLGVTAVELLPPHA
                     FVDDQFLVDKGLSNYWGYSTLNYFTPEPRYAESSDPLVQVSEFRRMVKELHSAGIEVI
                     IDVVYNHTCEGNHLGPALSWRGVDNATYYRLLPDNPRHYIDYTGTGNSLDVAHPQTLK
                     LVLDSLRYWVEEMHVDGFRFDLGVTLGREWPQFDAGSGFFDAIHQDPVLSAVKLIAEP
                     WDIGPRGYQTGAFPILWSEWNDRFRDEVREWWLKGGQDRGTMAYRMAGSSDIFRGSGR
                     GPRASINFIVAHDGFTLRDLVSYEEKHNEANGENNEDGHDHNQSANFGAEGHTNIPEI
                     NALRSRMQRNLLASLLLAQGVPMLAHGDEVNRSQNGNNNAYAQDNELTWMDWNLDEGD
                     LALYEFTSRVVALRESEPLLRRRRYFRGQPDHPSALKDVAWLKPDGSEMEHGDWIAET
                     DEPLIFRLSGTAIEEPDEFGIAIRTSSLLVVMHRGAEETEIVLPDPNGETNQKQWEVV
                     ISTDCVDGTTSGEMHRAGASLTVPARTVMVLRGASG"
     gene            complement(3192..3746)
                     /locus_tag="VGR22_05650"
     CDS             complement(3192..3746)
                     /locus_tag="VGR22_05650"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV2128087.1"
                     /translation="MTSHSLSHRTVSIQTMHASRPSIHPGWYSPVLAPEEEPVETPPE
                     VLFEVFTEILKIAGALDRSASEALEDLRLTAGAFTALVELGKIGDAGVAPSELARRLS
                     VARRTATLYVDILTRQGWAVREAHPGDRRMVLARLSDAGKRLLEEDGERYQRRLAELL
                     GDISPAQAERLRQLLGLIVTDRRS"
     gene            complement(3957..4631)
                     /locus_tag="VGR22_05655"
     CDS             complement(3957..4631)
                     /locus_tag="VGR22_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2128088.1"
                     /translation="MAAGETTRIPVLLADDHGVVRAGLRALLEAQADMAVVAEAEDGQ
                     QAVELAKTHRPNVAVVDLSMPHGGMDTISAITSLDIGVRVLVLTVHAEERYLLPVLEA
                     GGSGYVRKSSAHTDLLNAIRTVAKGEVFLDPAATKTLLRGYLGRAHAGDELDIGEVLS
                     EREREVVKLTAEGYTAQQGAQMLSLSPKTVETYRHRAMQKLGLTNRAELVRYALRAGL
                     LSDEDV"
     gene            complement(4636..5715)
                     /locus_tag="VGR22_05660"
     CDS             complement(4636..5715)
                     /locus_tag="VGR22_05660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV2128089.1"
                     /translation="MNDQWQRRLSEVPLLYKILLANGLIVVLGATVGTLVTAHTVSSP
                     LDPLSYTLITMFAFVGFVLSIAVNYVVLRAAFQPLYRLGEVAESIRQGDFSRRVRPST
                     FSDPMLARLGASFNETLDELGQDRDRLRELTSQIIDAQEDERKRIARELHDDTAQVLF
                     AQLLRLTALKSSEHEPVRACAEALEQSTVDAIEGVRRLALELRPPALDDLGLWAALAE
                     LAQRYEERYDLRVAYEWKGSRERLARDLELVLYRIAQEAMTNVVKHARASHVQLALSR
                     SADEVALSISDDGVGFDAGRVSRRDDRSLGLGIFGMIERSALVGGSLRVVPLHPRGTR
                     IEAIVPLALVERIMESATSGGKAKG"
     gene            5832..6323
                     /locus_tag="VGR22_05665"
     CDS             5832..6323
                     /locus_tag="VGR22_05665"
                     /inference="COORDINATES: protein motif:HMM:NF003819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEV2128090.1"
                     /translation="MASTASFDIVSSFDRQELRNAVDQAMREIRARYDLKDTGTEIEE
                     SDIEVIITTQSDYSLIAVRDILESKLIRRGLSLKILDYQKEEEASGARVRQVVRLREG
                     LNDEIAKKISKDIRDNFKKVKPQIQGDAIRVQGKSRDELQEVIQHVKASDWPVALQFV
                     NYR"
     gene            complement(6310..>6737)
                     /locus_tag="VGR22_05670"
     CDS             complement(6310..>6737)
                     /locus_tag="VGR22_05670"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128091.1"
                     /translation="QDVVFMGRQTTLPPGPVRIARETGAPLIPVFTRRVARGHELTIG
                     EPFHIERTSDVRADMAVGMERLVDFLEAGIISSLDQWVMFQRVWLEPQRVPVRVFPVG
                     SPLESELLERVATALPDPERLAHRRNRTRLPEQDDPTDS"
BASE COUNT         1295 a         2153 c         2104 g         1185 t
ORIGIN      
        1 gtcgtattgt catacatttg ccatgcgcac gagcagtgcc gattcgcgca cagggtcggc
       61 aatcccgtcc cgtccaaccg cggagcatca cccagtgacc atttcccggg gccgcccgct
      121 gaccatcctg tttttcctgc tggcgacggc catcggcgtg gccatcgtcc cactcgcacc
      181 cactccgggt gcggacgatc cgatggacac ggacttcgtc gactacatgc agccgcggct
      241 atcggtgctg ctcgattcgg cgcgggaggt cgaagggatg gtgagcgagc gttcccgcaa
      301 tctgttggcg ctgcgtgccg agtccgctcg aatcaacgct ctccttgaac agatcgacac
      361 ctacctcgcc gcccgcgagc cttctcccga agagcagttg gtcgccgacc tctaccgagc
      421 cggagcggat aacctcaggc tggcgatcga caccgcctcc gatgctctca gccgcttcga
      481 cttctcggca ataccggaga tgattccggt tttcacggcg ggcacggagc tgatcgagga
      541 atcgctggcg acgatgggga ctgagcagac acggcgttca tcgtacactg gtaccagcgt
      601 cgtctgactc ggcggcgcga tgtatgaatc cgtccagcgg tgcagtgaag gtgctgatgc
      661 gctgccgggt ttattcgaaa atgtcggaga catctagcga tgagcaccta caccgtccgc
      721 tatcagatcg gcggagatga gcacacggac cagctcgagg cggagaacgc ggcaaccgcg
      781 gcccgactcg tcgaggagcg ccatctcgaa gatgactggt tcgagttgat cgaggtccac
      841 ctggtcgagg aaggcgaatc cgacaccaat cccgagaaca gccattccgc cgaccaatcc
      901 acctaacctc cggctcccga tgcccgccga agcgggctgc ccggcttgcc ctgaggagct
      961 gtgaatgagc ctacctgaag tgttcggaac gccccgaccg ggccgtccga taccgctggg
     1021 tgcgcactgg gatggcgaag gaacgaactt cgccatctac tcacgccacg caagcgctgt
     1081 caccctttgc ctctatgaga atgacaacca cgagaagccg ttcgccacgg tgccgctgga
     1141 tgagcgcatg caggacgtct ggcatgtcta cttgccaggt gtccacgccg gcactcgtta
     1201 cggctacagc ttcgaagggg agtacgaacc tgccgaggga caccggttca accccaacaa
     1261 ggtgatgctg gatccgtatg cgaaggcgct tgccggcacc gtccaatggg atccggctgt
     1321 ctttccctac caactgggtg cggaagatga cgacgtcatg gacgagcggc ccaacgatgc
     1381 ctttgtgccc aaggctgtcg tgaccggcgg cgatttcaga tgggagggcg acccccgtcc
     1441 caacattccc tggaacgagt cggtcatcta cgagctgcat gtcaagggat tcaccgagtg
     1501 caacccggcg atccccgagc ggctgcgcgg cacttggcag ggcgtctcgc atgaggcgag
     1561 catcgagtat ctgaagaggc tcggcgtcac ggcggtcgag ctactgccgc cgcacgcgtt
     1621 cgtcgacgac cagtttctcg tcgacaaggg gctgagcaac tactggggct acagcacgct
     1681 caactacttc actccagagc cgcgatatgc cgagagcagc gatccgctgg tgcaggtgag
     1741 cgagtttcgg cgcatggtca aggagctcca ctccgccggc atcgaggtca tcatcgacgt
     1801 ggtctacaac cacacttgcg agggcaatca cctcggtccg gcgctcagct ggcgcggtgt
     1861 tgacaacgcc acctactatc gcctgctgcc ggacaatccc cggcactaca tcgactacac
     1921 cggaaccggc aactccctcg atgtcgccca cccgcagact ctcaagctcg tgctcgacag
     1981 ccttcgctat tgggtcgagg agatgcacgt cgatggcttc aggttcgacc tcggggtcac
     2041 acttggccgc gagtggccac agttcgatgc gggcagtggc ttctttgacg ctatccacca
     2101 ggatccggtg ctctccgctg tcaagctcat tgcggagccg tgggatatcg gtccacgcgg
     2161 ataccagaca ggggcgttcc cgatcctctg gtcagaatgg aacgatcgct tccgagacga
     2221 ggtgcgggag tggtggctga agggcggcca ggaccgggga actatggcgt accggatggc
     2281 ggggtcgtcg gacatcttcc ggggcagcgg tcgcggaccg cgcgccagta tcaacttcat
     2341 cgtggcgcat gacgggttta cgctgcggga ccttgttagc tacgaggaaa agcacaacga
     2401 agcgaacggc gagaacaacg aggacggtca cgaccacaac cagagcgcga acttcggcgc
     2461 cgaagggcat accaatattc cggagatcaa cgcgctccgg tcccggatgc agcggaacct
     2521 cctggcttcg ctgctgcttg cccaaggcgt gcccatgctc gcgcatggcg acgaggtaaa
     2581 tcgctcgcag aacggcaaca acaacgccta cgcgcaggac aacgagctga cctggatgga
     2641 ctggaacctc gacgagggcg atctggcgct ctacgagttc accagtcgcg tggtcgccct
     2701 gcgggagtcg gagccgctgc tgcgccgtcg ccgctacttc cgtggccagc cggatcaccc
     2761 gagcgccctc aaggacgtcg cgtggctcaa gccggacggc agcgagatgg agcacggaga
     2821 ctggatagcg gagactgacg agccgctgat cttccgtctc tctggtacag ccattgaaga
     2881 gccggacgag ttcggtatcg cgattcgcac ctcttcgctc ctggtcgtca tgcaccgcgg
     2941 cgcggaggaa accgagatcg tgttacctga tccgaatggc gaaacgaatc agaagcagtg
     3001 ggaagtggtg atctctaccg actgtgtcga tggaacgacg agcggtgaaa tgcaccgcgc
     3061 gggagcaagt ctgacggtac ccgcccgcac cgtgatggta ctgcgcggcg ccagtgggta
     3121 gcgccggatg gtggcacaac gagaacggga gcggctgctc ccgttctcgt tgtgcctttg
     3181 aagatgaccc gctagctacg cctgtcggtt acgatcagac ccagcagctg tcgcaggcgc
     3241 tcggcctggg ccggagagat atcgcccagc aactcggcaa gccggcgctg gtagcgctca
     3301 ccgtcctcct cgagcagccg cttgccggca tcgcttaggc gcgcgagcac catacggcgg
     3361 tccccggggt gcgcctcgcg tacggcccac ccttggcggg tgaggatgtc gacgtagagc
     3421 gtcgcagtgc gccgcgcaac ggacaggcgg cgggcgagct ctgaaggcgc cacgccggcg
     3481 tcgccgatct tgccgagctc aaccagcgca gtgaacgctc ccgcggtgag gcgcaaatcc
     3541 tcgagggcct ccgaagctga tcggtcaagc gcgccggcaa tcttgagaat ctcggtgaaa
     3601 acttcgaaga gcacctcggg tggtgtctcg accggctctt cttcgggggc cagcaccggg
     3661 gagtaccagc cggggtggat cgacggcctg ctggcgtgca tcgtctgtat ggagacggtt
     3721 cggtgagata gtgaatgact ggtcatagcc attgatgttt cccctcgtca tcgagtgcgg
     3781 tggcggtcca tactgcgaaa aggatgagtg ctcgatctgg gactgacgct cacagccgct
     3841 gaacttcttc agtatttcga gtttagggaa accggacctt cccgggcgac agggagagtt
     3901 cgcgctttcg cgcgtagaat ttcctgatga tgtcccacat actgtcggga atagccttac
     3961 acgtcctcgt cggacaatag gccggcccgc agggcgtacc ggaccagctc tgcccggttg
     4021 gtaagcccga gcttctgcat cgcgcggtgc cggtaggttt caacggtttt cggcgagagc
     4081 gagagcatct gcgccccctg ctgcgctgtg tatccctccg ccgtcagctt gacgacctcg
     4141 cgctcgcgct ccgaaagcac ctcgccgatg tcgagctcgt caccagcatg agcacgtccg
     4201 agataccccc tcaacagcgt cttggtagct gccgggtcaa gaaagacctc acccttggca
     4261 accgtgcgaa tggcgttcaa caggtcggtg tgcgcgctcg acttgcggac gtagccggag
     4321 ccgcccgctt cgagcacggg caggaggtag cgttcctccg catgtacggt caggacgagc
     4381 acccggacgc cgatgtcgag actggtgatc gccgaaatcg tgtccatacc accgtgcggc
     4441 atcgagaggt cgacgaccgc gacgttgggg cggtgggtct tcgccagctc caccgcctgc
     4501 tggccgtcct cggcctcggc aaccacggcc atgtccgctt gggcctccag gagcgcccgc
     4561 aggcccgccc tgacgacacc atggtcgtcg gcgagcaata ccgggatacg agtagtttcg
     4621 cccgcagcca tcctgctacc cttttgcctt cccaccagac gttgctgatt ccatgatgcg
     4681 ctcgaccagc gccagcggca cgatcgcttc aatgcgtgtg ccgcgcgggt gaagcggcac
     4741 cacacgtagg ctcccgccga ccagcgcgct tcgctcgatc atgccgaaga tgccaagacc
     4801 gaggctgcgg tcatcccgac ggctcacccg ccccgcgtcg aagccaacgc cgtcgtcgct
     4861 gatcgagagg gcgacctcgt cagcggagcg cgacagcgcg agctggacgt gggatgcacg
     4921 cgcgtgcttg accacgttgg tcatcgcttc ctgcgcgatc cggtaaagca ccagttcaag
     4981 gtccctggcg agccgctcac gcgacccctt ccactcgtag gcgaccctca ggtcatagcg
     5041 ctcctcgtac cgctgtgcca gctcggccaa cgccgcccac aggccgaggt cgtcgagggc
     5101 cggaggccgc agctccagag cgaggcggcg cacgccctcg atcgcgtcga ctgtcgattg
     5161 ctccagcgct tcggcgcacg ctcgaaccgg ctcgtgttcc gaggacttca gcgcggtcaa
     5221 acggagcagt tgcgcgaaaa gcacttgagc ggtgtcgtcg tgcagttcgc gagcgatccg
     5281 tttccgctca tcctcctgag catcgatgat ctggctcgtc agctcgcgca gccgatcccg
     5341 atcctgtccc agctcatcga gcgtctcgtt gaacgacgcg cctagccgcg ccagcatcgg
     5401 gtccgagaac gtcgagggcc gcacccggcg gctgaagtca ccctgccgaa tgctctcggc
     5461 gacctcgccg agccggtaca gcggctggaa cgccgcgcga agcacaacat agttcaccgc
     5521 aatgctcaag acgaagccaa caaacgcgaa catggtgata agcgtgtagc tcagcgggtc
     5581 caatgggctg ctcacggtat gtgcggtaac gagcgtcccg accgtggcgc ccagaacaac
     5641 gatcagcccg tttgccagca ggatcttgta aagaagtgga acctcagaga gccggcgttg
     5701 ccactgatcg ttcacgtcaa ctccttccca tgttctctat cgcgtcccga cgctgcaagg
     5761 atgaactgac cggcgtcgaa gcggtatggt atacagtcgc gagcatcgat acctcgacga
     5821 ggagggaaat tatggcaagt acagcatcgt tcgacatcgt atccagcttc gatcggcagg
     5881 agctccgtaa cgccgtggac caggccatgc gggagatccg cgcccgatac gatctcaagg
     5941 ataccggcac cgagatcgag gagtcggaca tcgaggtcat catcactacg cagtccgact
     6001 acagcctcat agcggtgcgt gacattctcg aatccaaact cattcggcgc gggctgtcgc
     6061 tgaagatcct cgactaccag aaggaagagg aagcttccgg cgcgcgcgtt cgccaggtcg
     6121 tcaggctcag ggaaggcctg aacgatgaga tcgccaagaa gatctcgaag gacattcggg
     6181 acaacttcaa gaaggtcaag cctcagatcc agggcgacgc gatccgcgtt cagggcaaga
     6241 gccgcgacga attgcaggaa gtcatccagc acgtgaaggc gtccgactgg cctgtggcgc
     6301 tacagttcgt caactatcgg taggatcatc ctgttcgggg aggcgggtgc ggtttctccg
     6361 gtgcgccagg cgctccgggt cgggcagcgc ggttgcgacg cgctccagca gctcgctttc
     6421 cagcggggat cccaccggaa agacgcgcac cggcacacgc tggggctcca gccacacccg
     6481 ctggaacatc acccattgat cgagcgacga aatgattccg gcctccaaga aatccacgag
     6541 tcgctccatc ccgaccgcca tgtcggccct gacgtccgat gtgcgctcga tatggaacgg
     6601 ctcgccgatc gttagctcgt gtcccctcgc gacacggcgc gtgaagacgg gaatgagcgg
     6661 cgcgcccgtc tcccgtgcga tgcgcaccgg tcccggtggg agcgtcgtct ggcggcccat
     6721 gaagaccacg tcctggc
//
LOCUS       DASYWL010000159         3311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_326233, whole genome shotgun sequence.
ACCESSION   DASYWL010000159 DASYWL010000000
VERSION     DASYWL010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326233"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VGR22_05675"
     CDS             <1..270
                     /locus_tag="VGR22_05675"
                     /inference="COORDINATES: protein motif:HMM:NF037014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128092.1"
                     /translation="DTVQLDTEEAPAGSRELLQPIVKNGTRVSTEESLQAIRERAMAS
                     LDALPAHLRELTPERQHSVEMSPALLDLRQQTIAHYQAQLADAPD"
     gene            complement(369..1646)
                     /locus_tag="VGR22_05680"
     CDS             complement(369..1646)
                     /locus_tag="VGR22_05680"
                     /EC_number="1.1.99.24"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047988 - hydroxyacid-oxoacid
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid-oxoacid transhydrogenase"
                     /protein_id="HEV2128093.1"
                     /translation="MALETVFAMESSAIKFGSGATREIGDDAQVRGCRRVMLLTDPKL
                     AESPILDSARIALADAGVDCDIFSDVFVEPTDDSFRAAIDFALAGTYDGFVAVGGGSV
                     IDTAKAANLYSTHPADFLAYVTKPIGEGRVVPGPLKPFIAVPTTAGTGSETTGNAVLD
                     FPQIGTKTAIAHRHLRPSLGVIDPDNVRDLPPMVAACSGLDVLCHALESFTAVPFDER
                     PKPEKPQLRTPYQGSNPVSDIWSKEAIRIGAERLKAAVNDPSDHEARGQMLFAATLAG
                     IGFGNAGVHLPHALSYPISRLSRGYFPPDYPHDHSLVPHGMSVVLTAPAIFLWTADAA
                     PQAHREAAILMGASPDEVDERNAGDAIAERFVALMKAIGMPNGLKGVGITEDDLDVLV
                     AGTLPQGRLLVISPKEPTEADIRQLFMDSMTLW"
     gene            1806..3125
                     /locus_tag="VGR22_05685"
     CDS             1806..3125
                     /locus_tag="VGR22_05685"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEV2128094.1"
                     /translation="MEFLIVFLLIVLNGLLAMSELAVVSARTSRLQQRADAGSAGAQA
                     ALELVAQPDRFLSTVQIGITLIGVGTGALGGATLATPVGDLLAEIPGVGPTTGRSIAG
                     VVVVLGITYLALVIGELVPKRIALQRAEAMAILMSRPLKTLSKVTAPVVSVLAVSSDV
                     VLRLIGQHGDTSDQISDEDVHHLLLEGRKAGVFEAAETRMVAGVFDIGDRTAAEIMTP
                     RHRLAFLDLRWSDEDNRRVMREAPHSHYPVVEGSIDHVVGMVSTRDLWQQALRSERCD
                     LRAAMTEPLFVPELGAVLDILEQMRTSGRRDAIVVDEYGGVVGLLTLTDVFSDVVGEL
                     DDQTNTGIKGAVRRDDGTWLIDGGLPAHEARELLDIDELPGEEDGHFETLGGFVIEQL
                     GHIPEESEYVDHEGYRIEVADMDGHRIDKLLVSQVADAATTDGADSI"
BASE COUNT          556 a         1078 c         1093 g          584 t
ORIGIN      
        1 gataccgtcc agctcgacac cgaagaggca ccggcgggaa gccgcgagct gctgcagccg
       61 atcgtcaaga acggtacgcg ggtttcgacg gaggaatcgc tgcaggcgat ccgcgagcga
      121 gcaatggcgt cgctggatgc cctgcccgcc cacctgcggg agctcacccc ggagcggcag
      181 cactccgtcg agatgtcacc ggccctgctc gacctccggc agcagactat cgcccactac
      241 caggcgcagc ttgccgacgc tccggattga ttccctcccc ctcgccaccc ggcgttccgc
      301 agcaactcgg ggcgatccgg taatggccgg ctgtggtctc ctgtattcgg gtcgcgaaag
      361 gccggtccct accagagcgt catgctgtcc atgaacagct gacggatgtc ggcctccgtc
      421 ggctccttcg gcgagatgac cagcaggcgt ccctgcggca gcgtccccgc caccagcacg
      481 tcgaggtcgt cctcggtgat gcccacgccc ttgagcccgt tcggcatccc gatcgccttc
      541 atcaacgcga cgaatcgctc ggcaatcgcg tcgcccgcat tccgctcgtc cacctcgtcc
      601 ggtgaagcgc ccatcaggat cgccgcctct cggtgcgcct ggggcgcagc gtccgccgtc
      661 cacaggaaga tcgccggcgc tgtcagcacg accgacatgc cgtgcggcac gagactgtga
      721 tcatgcgggt agtccggcgg gaagtagccg cgcgagagcc gcgagatcgg gtacgacagc
      781 gcgtgcggta ggtggacgcc ggcgttgccg aagccgatgc cggccagcgt cgccgcgaac
      841 agcatctgcc cgcgcgcctc gtggtcgctc gggtcattga cggcggcttt gagccgttcc
      901 gcgccgatcc ggatcgcctc cttcgaccag atgtccgata ccgggttcga cccttggtac
      961 ggcgtcctca gttgcggctt ctccggcttc ggacgctcgt cgaacggcac ggccgtgaac
     1021 gactccagcg catggcagag cacatccagt ccggagcacg cagcaaccat cgggggcagg
     1081 tcgcgcacgt tgtccgggtc gatcacgccg agcgaggggc gaagatgccg gtgggcgatc
     1141 gccgtcttcg tgccgatctg ggggaaatcg agcacggcgt tgccggtcgt ctcgctgccg
     1201 gtcccggcgg ttgtcggcac ggcgatgaac ggcttgagcg gaccgggtac aactcttccc
     1261 tcgccgatcg gtttggtgac gtaggcgagg aagtccgcag ggtgggttga gtagaggttg
     1321 gcagcctttg ccgtgtcgat cacagagccg ccaccgacag cgacgaaacc gtcgtacgtg
     1381 ccagcgaggg caaaatcgat cgccgcacga aacgagtcgt cggttggctc aacaaagacg
     1441 tcggagaaga tgtcgcagtc cacaccggcg tctgccagtg cgattcgtgc cgagtcgagg
     1501 atcgggctct cagccagttt cgggtccgtc aacagcatca cccgacggca accgcgaacc
     1561 tgagcgtcgt cgccgatctc ccgcgtcgcg ccggagccga acttgatggc cgagctctcc
     1621 atcgcgaata ccgtttccag tgccatgccg tgccctcctc gtggcgtgtt tccgtcccag
     1681 tgtacgatgg acatgcagcc cggccgctcg accatcgctc gccgggcgtg tgccaatacc
     1741 tgacacacgc actctatcgc agaactcctg cgttgcggtg ccgacaaggg gtccttgtgg
     1801 tcggaatgga attcctgatc gtctttctgc tgatcgtgtt gaacggcctt ctggcgatgt
     1861 cagaactggc ggtcgtctcc gccaggacat cgcggcttca gcagcgcgcg gacgcgggaa
     1921 gcgccggcgc gcaggcagcg ctggagttgg tcgcgcaacc ggatcggttc ctctcgacgg
     1981 tacagatcgg catcacgctg atcggcgtcg gcaccggtgc gctcggtggt gccacgctcg
     2041 ccacaccggt gggcgacctg ctggccgaga tccccggtgt cggcccgacc accggcagga
     2101 gcatcgccgg tgtggtggtg gtgctcggca tcacctacct cgcgctcgtc attggagagt
     2161 tggtccccaa acgcatcgcc ctgcagcggg ctgaagcgat ggccattttg atgtcccgtc
     2221 cgctgaagac actgtcgaag gtcaccgccc ctgtcgtgag tgtcctggcg gtctcgagcg
     2281 atgtcgtgct gcgtctgata ggtcagcatg gcgatactag cgatcaaatc agtgacgagg
     2341 atgttcacca cctgctgctt gagggccgga aggcgggtgt cttcgaagct gccgaaacgc
     2401 ggatggtggc cggtgtattc gatatcggcg atcgtaccgc cgccgagatc atgaccccgc
     2461 gacaccggct cgcattcctc gatctgcgct ggagcgacga ggacaaccgg cgagtcatgc
     2521 gggaggcgcc gcacagccac taccccgtgg ttgagggatc gatcgaccac gtcgtcggga
     2581 tggtgtcgac gcgcgacctg tggcagcagg cgctacgcag cgagcgctgc gatctgcgtg
     2641 cagcgatgac cgagccgctg tttgtgcccg aattgggagc ggtgctggac atcctcgagc
     2701 agatgcggac cagtggccgg cgagacgcga tcgtggtcga cgaatacggc ggagtcgtcg
     2761 ggctcctgac actcacggac gtcttctcgg acgtcgttgg ggagctcgac gaccagacca
     2821 acaccggcat caagggggcg gtgcggcgcg acgacgggac atggctaatc gacgggggac
     2881 ttccggcgca cgaggcgcga gagctgctcg acatcgacga gcttcccggc gaggaggatg
     2941 ggcacttcga gacgctgggc ggcttcgtca tcgagcagct tgggcacatt cccgaggaga
     3001 gcgagtacgt cgaccacgaa gggtaccgca tcgaggtcgc cgacatggat ggtcatcgca
     3061 ttgacaaact actcgtgagc caagtcgcgg atgcagctac caccgatggg gctgacagca
     3121 tatagcgaac ggcccccggt gagaactcca ggggccgttt cgtccgcctt cttgctggct
     3181 ggcagctatt tgccgagcct gctggcgacg agatcgcgaa gccgctcgac atccttgcga
     3241 tcgcgctcct ccatctcctc gatggccttc agcgcctcct ggtcgccctg cgcgtcctgc
     3301 ttgtatacct c
//
LOCUS       DASYWL010000160         7669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_439023, whole genome shotgun sequence.
ACCESSION   DASYWL010000160 DASYWL010000000
VERSION     DASYWL010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7669
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439023"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(322..1242)
                     /locus_tag="VGR22_05690"
     CDS             complement(322..1242)
                     /locus_tag="VGR22_05690"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128095.1"
                     /translation="MAYLNTTLGPKAADELGMILPHEHVFVDLRTWDQPGYGEGGPED
                     VVRLMKPEIEAARVAGVTAIVEPSTVGVGRRTDILLAVSEATGFPLVAPTGVYREPWL
                     PPWAREASEDALREWMIAELTGEIEGTGVQAGWIKVGSTDDGATEMEAKVLRAAAGAA
                     IETGSTIGSHTVRGHVARNQLDIIESVGARPDTFVWIHAHQEPDVAVHHELGRRGAWI
                     EYDGIGEPADDKRFIDLVQCGLDAGLEERLLLSQDRGWYDPAQPGGGTPKPFTYLPEH
                     FLPKLADAGIDDATIDQLMRRNPFDAFARP"
     gene            complement(1260..1835)
                     /locus_tag="VGR22_05695"
     CDS             complement(1260..1835)
                     /locus_tag="VGR22_05695"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2128096.1"
                     /translation="MRNAVKIGERVWLRPLELADARAIALAGHVETERVSRRVPTSVI
                     AFERWVRGFATDGAPDAIAFAVCWHGDDTCIGTVRLGQIDWVNRTAETGSGLLSAEDR
                     GRGIGTEAKHLLLEYAFVDLGLHALNAMVYEGNTRSAAALEKQGYRLAGRLTADVQRD
                     GAFRDTLVFDLTREDWERARERRRSSHSDIP"
     gene            complement(1828..2382)
                     /locus_tag="VGR22_05700"
     CDS             complement(1828..2382)
                     /locus_tag="VGR22_05700"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEV2128097.1"
                     /translation="MSETGAWPVAGYLQDGRYGLRAPVPEDADYVTTWHEEPFPMTLE
                     AARDLLIQQETIPWGNNPAIRLMIVDLVTDAVVGGAIVERDDNRVSKLVITVGGPDPT
                     GEAAQRVRAAVLRLLVPWVMGELSLMTTVIDVPADETILIDAAKDLGMVEAVRLREHI
                     LRPSGRVDLLMLELVNRQWGRSDA"
     gene            complement(2463..3710)
                     /locus_tag="VGR22_05705"
     CDS             complement(2463..3710)
                     /locus_tag="VGR22_05705"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2128098.1"
                     /translation="MSGRVRVASPRLLLLQTTPDARLILIARGVRAFGDGFVSVLLPL
                     HLTTVGLPAGQVGLITTATLLGSAMLTLLAGALADRWKRHDLLVRASLLMIFTGVGFA
                     VADDFWPLLAVAFVGTMNPTAGDVSVFLPTEQSLLSVTVAPEHRTALFARYTLIGVLV
                     AAVGALAAGIPDLLTSILPMSLSRALDAMFVLYAALGVVTLAINHRLSEDVEPPADAP
                     PSRLGSSRRAVYQMAAVFSLDAFAGGFTMNAIIAFWLFLRFDLATSTAGLIFFWAGLL
                     SAGSQLVAPMLAQRIGLINTMVFTHLPANVFLLLTPFMPNVELAVACLLGRFALAQMD
                     IPARTSYVMAVVTPPERPAAASITNVPRSLAPAVSPTFAGKLLGMTTFGWPLVIGASL
                     KIVYDLLLLAMFRHRSPPEEQHR"
     gene            complement(3873..4535)
                     /locus_tag="VGR22_05710"
     CDS             complement(3873..4535)
                     /locus_tag="VGR22_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128099.1"
                     /translation="MDVGDIISLLFVAFLILSLLGGLFAGSQESEEGQRRVPRPTDIF
                     ESGGPQRPQQSSQPQRAPQERQMPTTATEWWQELTAQRSGTESGPQPRPQPQPQAPSR
                     PEPRQPERQVATYDDAIERGEVDRAERQQRRRERETAAAGQSVRTTEQETLRNEITDR
                     RSPIDAEPRRRRDREIGAVTARRHRAGGDILRRSLKEPETLERAFIVKEVLDVPIALR
                     EK"
     gene            complement(4608..5558)
                     /gene="floA"
                     /locus_tag="VGR22_05715"
     CDS             complement(4608..5558)
                     /gene="floA"
                     /locus_tag="VGR22_05715"
                     /inference="COORDINATES: protein motif:HMM:NF010186.0"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HEV2128100.1"
                     /translation="MEENIGLIIGIVVLALFILFFFYFVPVGLWITAIFSGARVGLGT
                     LIGMRLRKVNPSEIVRPYISAIKADVNLHVSQMEAHYLAGGNVGRVVQALISADRANI
                     ELPWKRATAIDLAGRDVLEAVQVSVNPKVIQTPRVSAVAKDGIQLVAVARVTVRANID
                     RLVGGAGEETIIARVGEGTVSSIGSAASHKTVLENPDQISRYVLERGLDAGTAFEILS
                     IDIADVDVGRNIGAELQTDQAEADKKIAQAKAEERRAMAMAAEQEMRAKVVEAEAQVP
                     LAMAEAFRSGNLGVMDYFKMENVQADTRMRGSIAGDEDHQ"
     gene            complement(5644..7014)
                     /locus_tag="VGR22_05720"
     CDS             complement(5644..7014)
                     /locus_tag="VGR22_05720"
                     /inference="COORDINATES: protein motif:HMM:NF014074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128101.1"
                     /translation="MRDRTMWRGWTIIGALGRALVLIGLMLALVTATFSPGATAQGDG
                     EPQVMIIPIEGAVEPGLGAYLDRSIEEAEESGIATIILDIDTPGGRLDTVLEMRDAIL
                     DTAIPVVAFVNRQAFSAGALITIASDEIWMTPGAVFGAATPISGATGETADEKTISAV
                     RSTFRATAEEQGLDPEIAEAMVDPNVVVEGLDGPNSLLTLTTEQALAYDYADGVAQSR
                     EDLLAELGFEDVEVVVASPTFFERVVRFITDPALASILILLGLAMVVLDGLVGGFGVM
                     ALIGAGLLGIFFYGHLLAGFAGWEDVVLVVVGLILIAIEVFVIPGFGVAGLLGGISLI
                     GGLVLAMTSRDFGDEGFSAEAGDALQTLLITLSLTVLVIVLVAWGLPRLVPSVARPAR
                     GLQRMTLSSTVSQGNDRESKPGYFTRLLGGHDVVERDTEGHTAVERRRRADPGSPRDP
                     ARDRHI"
     gene            complement(7332..7619)
                     /locus_tag="VGR22_05725"
     CDS             complement(7332..7619)
                     /locus_tag="VGR22_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128102.1"
                     /translation="MRDPSHVRSLTEAEWVALLERNGFRIETVEAFPKRHEFDDWTGR
                     MQVSEADRERLQAMMREVGETIWREFEVAFDPETGRVISWVDTKTLFTART"
BASE COUNT         1339 a         2620 c         2479 g         1231 t
ORIGIN      
        1 ctcctgcgcg ccgaagatgc gctggagcag ccggttgcga ctggtatcac gaacgccgat
       61 gagcgcgttg accttccccg gtacggcgac ctgtgccacc cccaggccca ggctgaacca
      121 tccgagccac tggcgagctt acgtgcggtg tcggtattga tcgggacgga gggcatcgcc
      181 atgtatagcc tcctcgcatg catgtgatcc cgctggacac gggcgcctag cgaatctggc
      241 gactgaccct gccgccagca catctggatg cgggagcgaa acgcaagagc cgtgccggtc
      301 acggacgggg ttgctgtcag ttcacggccg ggcgaaggca tcgaaggggt tgcggcgcat
      361 cagctgatcg atggttgcgt cgtcgatgcc ggcgtcggcg agcttgggca ggaagtgctc
      421 cggcaagtag gtgaagggct tcggggtgcc gccgcccggc tgggccgggt cgtaccagcc
      481 gcgatcctgg ctgagcagga gccgctcctc cagaccggcg tcgaggccac actggaccag
      541 gtcgatgaag cgcttgtcgt ccgcgggctc cccgatgccg tcgtactcga tccacgcgcc
      601 acggcgaccg agctcgtggt ggaccgccac gtccggctcc tgatgggcat gaatccagac
      661 gaaggtgtct ggccgtgcgc cgaccgactc gatgatgtcg agctggttcc gggcgacatg
      721 gccgcgcacg gtgtggctcc cgatggtgga accggtctcg atcgcggcgc ccgccgccgc
      781 gcgcagcacc ttcgcctcca tctcggtcgc gccgtcgtcg gtggagccga ccttgatcca
      841 gcctgcctgc acgccggtac cctctatctc accggtaagc tcggcgatca tccactcgcg
      901 cagggcatcc tcactcgcct cccgcgccca cggcggcagc cacggctccc ggtagacacc
      961 agtcggcgcg acgagcggga accccgttgc ctccgagacg gccagcagga tgtccgtccg
     1021 gcgcccgacc ccgaccgtgc tcggctcgac gatcgccgtc accccagcga ctcgagccgc
     1081 ctcgatctcc ggcttcatca accgcacgac atcctccggg ccaccctcac cgtaccccgg
     1141 ctgatcccac gtccgcaggt cgacgaagac atgctcatgc ggcaggatca tgccgagctc
     1201 gtcggctgct ttcggtccaa gggtcgtgtt caggtacgcc atggaaaccc cctcaacgat
     1261 tacggaatat ccgaatggct ggaacgccgc cgttcgcgcg ctcgttccca gtcctcgcgg
     1321 gtaaggtcga acaccagcgt atcgcgaaag gccccatcgc gctgcacgtc ggcagtgaga
     1381 cgtcctgcca ggcgataccc ctgcttctcc agcgccgccg cagagcgggt attgccctcg
     1441 tagaccatgg cgttgagcgc gtgaagcccg aggtcgacga atgcgtattc gagcagcaga
     1501 tgcttcgcct cggtgccgat ccctcggccg cgatcctcgg ctgagagcaa gccggatccg
     1561 gtttcggccg tgcggttgac ccagtcgatc tgtccgagcc gtacggtgcc gatacaggtg
     1621 tcgtcaccgt gccagcagac ggcgaaggca atcgcatcgg gagcaccgtc ggtggcaaag
     1681 ccgcgcaccc atcgctcgaa ggcgatgaca ctggtcggca ccctccggga aacgcgttcc
     1741 gtttcaacat gaccggcgag cgcgattgcc cgggcatcgg caagctccag cgggcgcagc
     1801 cagacccgct cgccgatctt cactgcgtta cgcatcgctt cgcccccact gccggttgac
     1861 caactccagc atcagcaagt cgacgcgtcc ggagggccgg aggatgtgct cgcgcaggcg
     1921 aacggcctcg accatgccga ggtccttcgc ggcgtcgatc aggatcgtct cgtcggccgg
     1981 gacgtcgatc acagtcgtca tgagactgag ctcgcccatc acccagggaa ccagcaggcg
     2041 gagcacagcc gcccgcacgc gctgcgccgc ctcgccggtc ggatccgggc caccgacagt
     2101 gatcaccagc ttgctcacac ggttgtcatc acgctcgaca atcgcccctc cgacgaccgc
     2161 gtccgtcacg agatccacga tcatgagccg gatggctggg ttattccccc aggggatcgt
     2221 ctcctgttgg atcagcaggt cgcgcgcggc ttccagcgtc atcggaaacg gttcctcgtg
     2281 ccaggtggtg acgtaatccg cgtcctctgg caccggtgcg cgcaacccgt agcgaccatc
     2341 ctgcaggtag ccggcaaccg gccatgcccc ggtctcactc atcccgatct ccctcacgcc
     2401 tgatgatccg atgtgccttc caccatgata gagctccatc tcgcggccga ggccagattc
     2461 gatcaccggt gctgctcctc cggcgggctg cggtgacgga acatggcgag cagcagcagg
     2521 tcgtagacga tcttgagtga tgccccgatc accagcggcc acccgaaggt cgtcatgccg
     2581 agcagcttgc cggcgaaggt cgggctgacc gccggagcga ggctgcgggg cacgttggta
     2641 atgctcgcag cggcgggtcg ctccggtggg gtgacgacgg ccatgacgta cgaggttcgg
     2701 gccgggatat ccatctgcgc cagcgcgaac cgtcccagca ggcaggccac tgccagctcg
     2761 acgttcggca tgaatggcgt cagcagcagg aagacattgg ctggcaggtg tgtgaagacc
     2821 atcgtgttga tcagcccgat ccgctgcgcc agcatcggcg cgaccagctg tgacccggcc
     2881 gagagcagtc ccgcccagaa gaagatcagc ccggcggtcg aggtcgccag gtcgaaccgc
     2941 aggaagagcc agaaggcgat gatcgcgttc atcgtgaacc cgccagcgaa cgcatcgaga
     3001 ctgaagaccg ccgccatctg gtagaccgcc ctgcgggaag atcccagacg cgacggtggt
     3061 gcgtcagcgg gtggctcgac atcttccgag agccgatgat tgatcgccag cgtgacaacg
     3121 ccgagcgcag cgtagaggac gaacatcgcg tccagcgccc gggagagcga catcggcaag
     3181 atagaggtca gcagatcagg gatgcccgct gcgagcgcgc cgaccgccgc aacgagcacg
     3241 ccgatcagcg tgtagcgggc gaaaagcgcg gtgcggtgct ccggcgcgac ggtcaccgac
     3301 aacagggatt gctcggtcgg caggaagaca ctaacgtcgc cggcagtcgg gttcatggtg
     3361 ccgacgaagg cgaccgccag cagcggccag aagtcgtccg cgacggcaaa cccgacgccg
     3421 gtgaagatca tcagcaggct cgcacggacg agaaggtcgt gtcgcttcca gcgatcggcc
     3481 agcgcaccgg ccagcagggt aagcatcgcc gaaccgagca gtgtcgcggt ggtgatgagg
     3541 ccgacctggc cggcaggaag cccgacggtg gtgaggtgga gcggcaacag cacgctgacg
     3601 aaaccatcgc cgaacgcacg gacgccgcgt gctataagga tcagtcgggc atcaggggtc
     3661 gtctggagca gcagcaatcg cggcgaagcg acgcgaactc gcccactcat ggagccgtgg
     3721 ttgcagcatg ggcggtggcg ctcatgctgt aactgtcacc ggcttcgcag acaatcacaa
     3781 gagggaggca gacgctacgc aggaatcgca aggacaagcc ctgtcagccc ggccacccac
     3841 ggccacggca gcggtttcgc aggttgcctg gcctacttct cccgaagggc gatggggacg
     3901 tcgagcactt ccttgacgat gaaggcgcgc tccagcgtct ccggctcctt gagtgagcgg
     3961 cgcaggatgt ccccgccggc gcggtggcgt cgagccgtca ccgccccgat ctcccggtcg
     4021 cgccgccgcc ggggctcggc gtcgatcggc gagcgcctgt cggtgatctc gttcctcagc
     4081 gtctcctgct cggtcgtgcg caccgactgc ccggccgccg ccgtctcacg ctcgcggcgg
     4141 cgctgctgcc gctcggcacg gtccacctca ccgcgctcga tggcgtcgtc gtaggtggcg
     4201 acctgccgct cgggctggcg tggctcgggg cgcgacggcg cttggggctg cggttgcggc
     4261 cgtggctgcg ggcctgattc ggtccctgat cgctgcgcgg tcaactcctg ccaccactcg
     4321 gtggcggtgg tcggcatctg ccgctcctgc ggcgcgcgtt gcggctgcga cgactgttgc
     4381 gggcgctgcg ggccgccgct ctcgaaaatg tcggtcgggc gcggcacgcg gcgctgcccc
     4441 tcctcgcttt cctggctgcc agcgaagagc ccgccgagca ggctgagtat gaggaacgcg
     4501 acaaagagca gactgatgat gtcgccaaca tccatggtcg aggactttcg ctggctcagg
     4561 caccacgaga tggtgcctga gcgatcgaga cggccgggga gcgagcccta ctgatggtcc
     4621 tcgtcgccgg cgatcgaacc gcgcatgcgg gtgtcggctt ggacattctc catcttgaag
     4681 tagtccatca ccccgaggtt gccgctgcgg aacgcctcgg ccatggccag cggcacctgg
     4741 gcctcggcct cgaccacctt ggcgcgcatc tcctgctcgg ccgccatcgc catcgcgcgg
     4801 cgctcctccg ccttggcctg ggcgatcttc ttgtccgcct cggcctggtc ggtctggagc
     4861 tcggcaccga tgttgcgccc gacgtcgaca tcggcgatgt cgatcgagag gatctcgaac
     4921 gccgtccccg catcgaggcc gcgctccagc acgtacctcg aaatctggtc cggattctcc
     4981 agcaccgtct tgtgcgatgc cgccgagccg atgctggaaa cggtaccctc accgacacgg
     5041 gcgatgatgg tctcctcacc ggcaccaccg acgaggcggt cgatgttggc gcgcaccgtc
     5101 acgcgggcga cggcaacgag ctggatcccg tccttggcga ccgccgacac gcgcggcgtc
     5161 tggatcacct tcgggttgac gctgacctgc acggcctcga gcacgtcacg accggcgagg
     5221 tcgatggcgg ttgcccgctt ccacggcagc tcgatgttgg cgcggtcggc cgagatgagg
     5281 gcctgcacca cacggccgac attgccgccg gcgaggtagt gcgcctccat ctgactcacg
     5341 tggaggttga cgtccgcctt gatcgcactg atgtacggcc gcacgatctc acttggattg
     5401 accttgcgca ggcgcatccc gatgagcgtg ccgaggccga cccgcgcgcc ggagaagata
     5461 gccgtgatcc acagccccac cggtacgaag tagaagaaga acaggatgaa cagggccagt
     5521 acgacgattc caatgatcag gccgatgttt tcttccatgc tgcattcctg tgcgtcgcga
     5581 catcacgttg ctcgcaacgt cgcgtcttga tacggtgccg ccagcgcggc ctcagtgagt
     5641 ccctcagatg tgccgatcac gagcgggatc gcgtggactc ccgggatcag cccgacgtcg
     5701 ccgctcaaca gcagtatgcc cctcagtgtc acgctccacc acatcgtgtc cgccgagcag
     5761 acgggtgaaa taacctggct tcgactcgcg atcattccct tggctgacgg tggacgagag
     5821 ggtcatgcgc tgaagacctc gcgccgggcg cgccacactg ggcaccagcc gtggcagccc
     5881 ccaggccacc agcacgatga ccaacacggt cagcgagagg gtgatcagca gcgtctgcaa
     5941 ggcatcaccg gcctcggcgc tgaagccctc gtcaccaaag tcacggctgg tcatcgccag
     6001 caccagcccg ccgatcagcg agatgccgcc aaggagccca gcgacgccga accccgggat
     6061 cacgaagacc tcgatggcga tcaggatgag cccgaccacg accagcacga catcctccca
     6121 gccggcaaag ccggcaagca ggtgcccgta gaagaagatg cccagcagcc ccgcgccgat
     6181 cagcgccatc acaccgaacc cgcccacgag gccgtccagc accaccatcg cgaggccgag
     6241 caggatcagg atcgatgcca gcgccgggtc ggtgatgaag cgcaccacgc gctcgaagaa
     6301 ggttgggctg gcgacgacca cctccacgtc ctcgaagccc agctccgcca gcaaatcctc
     6361 gcggctctgg gcgactccgt cggcgtagtc gtacgccagc gcctgctcgg tagtgagcgt
     6421 gaggagcgag ttgggaccat ccaagccctc gaccacgacg ttcgggtcga ccatggcttc
     6481 cgcgatctcc gggtcgagcc cctgctcttc cgcagtggcg cggaaggtcg agcgcacggc
     6541 ggagatggtc ttctcatcgg cggtctcgcc ggtcgccccg ctgatcgggg tggccgcacc
     6601 gaagacggca ccgggcgtca tccagatttc atcactggcg atggtgatca gcgcgccggc
     6661 agagaaggct tgccggttga cgaaggccac gacggggatg gcggtgtcca gaatggcatc
     6721 gcgcatctcc agtacagtat cgagccgccc gccgggggta tcaatatcga ggatgatggt
     6781 ggcgataccg ctctcctcgg cctcctcgat cgagcgatcg aggtaggccc cgaggccggg
     6841 ctcgaccgcc ccctcgatcg ggatgatcat cacctgtggc tcaccatcgc cctgtgcggt
     6901 cgctcccgga gagaacgtcg ccgtcaccaa ggcaagcatc aggccgatca gcacaagcgc
     6961 gcgaccgagc gcaccgatta tggtccaccc ccgccacatt gtcctgtcgc gcatcatgcg
     7021 cccccttttc gtctgcatca ttgggaaaat ggtagcagag agccggagtt gcaccttcgg
     7081 cggcgctacc gtggttcgag aaaaacgccc cgttgatgca gcaattccgc acgccttctc
     7141 aacgaccatt ccttggccgc cgaggggctg agtccgatca cgggagaaat ttctccactc
     7201 cgcggcttcg ccgctccggt cgaaatgacc ggttggggta tggcggagcc gcatgcccac
     7261 gacgacgggc ggacacgaaa acctggactg agcgcagaat acttgcccgc ccctgcgatt
     7321 ggattagacg tctacgttcg ggcggtgaag agggtcttgg tgtcgaccca agagatgaca
     7381 cgaccggttt cggggtcgaa ggcgacctcg aactcccgcc agatcgtctc accgacctcg
     7441 cgcatcatcg cctgcagccg ctcccggtcc gcctcgctca cctgcattct gccggtccag
     7501 tcgtcgaact cgtgccgctt cgggaaggct tccacggttt cgatccggaa cccgttccgc
     7561 tccagcagcg ccacccactc cgcctcagtc aaggagcgga cgtgcgaggg gtcgcgcacg
     7621 gtctcgtagg cgttgaagaa gtcgcccgcc gcgccctacg gtacggtgc
//
LOCUS       DASYWL010000161         2189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_63835, whole genome shotgun sequence.
ACCESSION   DASYWL010000161 DASYWL010000000
VERSION     DASYWL010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63835"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VGR22_05730"
     CDS             <1..492
                     /locus_tag="VGR22_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV2128103.1"
                     /translation="ARLYTLPETLPDIHVAADGPESATLAGKIGNGLISTSPKKETVE
                     SFRGAGGTGKPTYGQMTVCWAKTEDEGLETALKIWGYTGLPGQLSQELALPQYFQEAA
                     QLVTKETIGQSVVCGPDAERYHQQIKAYADAGFENVYIHQIGPDQDGFFDFAKREILP
                     KYQ"
     gene            827..1192
                     /locus_tag="VGR22_05735"
     CDS             827..1192
                     /locus_tag="VGR22_05735"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEV2128104.1"
                     /translation="MPDLPQPSHGEVWTVVLDPEKGHEQGGARPAVVISNDRFNRAFE
                     SLIIVVQMTRTNRGIPTQIPVDPPEGGLTAPSFIMCEQVRAVSIAGFRGRRGAIRDET
                     MMKVEVMLPHLIARLPDRR"
     gene            1291..1710
                     /locus_tag="VGR22_05740"
     CDS             1291..1710
                     /locus_tag="VGR22_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128105.1"
                     /translation="MQVDDLRLALQWSGARLPSEAATEALYYFARTPGVWSQPADRLR
                     DALIDVGRAMTDAIAIVVGNHVAQGDTVVIEGDGILPELLEHTEVRPHAASGAARMAL
                     LVPDSEHALLRAMLDRGRGIAEIGATPEARRAAVASW"
     gene            1828..>2189
                     /locus_tag="VGR22_05745"
     CDS             1828..>2189
                     /locus_tag="VGR22_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128106.1"
                     /translation="MTAPFESFFRSREHARDTYLSRLFALFSEQVVRVWCMCPQAPYE
                     DIGRPTLYEPGVSRGSTLDFTLRDRASGRTFVSELKCELEYDNYRYLRLTHASQLAHP
                     TSTAFQRLLLAAKEPPALG"
BASE COUNT          406 a          676 c          753 g          354 t
ORIGIN      
        1 gcccggctct acacgctccc cgagacgctg ccggacatcc acgtcgcggc ggacgggccg
       61 gagtcggcca ccctggcggg caagatcggc aacggtctca tctccacatc gccgaagaag
      121 gagacggtgg agtccttccg gggcgccggc ggcacgggca agccgaccta cggccagatg
      181 accgtctgct gggcgaagac cgaggacgag gggctggaga cggcgttgaa gatttggggc
      241 tacactgggc tgccgggcca gctctcgcag gagctggcgc tgccacagta cttccaggag
      301 gcggcgcaac tggtcacgaa ggagacgatc ggccagtcgg tggtctgtgg gccggacgcc
      361 gagagatacc accagcagat caaggcctat gccgacgccg ggttcgagaa cgtctacatc
      421 caccagatcg gaccggacca ggacggcttc ttcgacttcg ccaagcgcga gatcctgccg
      481 aaataccagt gatcggcaga ccgcgaacgc cagacgcccc gggccactcg gtccggggcg
      541 tttcgttgta tgtcagcatg gaaatgtagc aatgagccta tccttgcgtg catatacaac
      601 agcgggcaac caaaggggaa ctgcgacgga aggtcgccag cacaacgatc cacatcgtcc
      661 cggcgtcgcg agcagttctg gcaggagctg ggggtttcgc tcgccaggct caagcacgat
      721 ccggaggcct ggcaggacta tcaggacgaa actgcgctct gggacacggc cgccggtgat
      781 gagctcgagg gggaaattcc ctacgacacc gagagtgacg accccgatgc ctgatctgcc
      841 gcaaccgagc catggtgagg tgtggaccgt cgtgctcgac ccggaaaagg gccacgagca
      901 gggcggcgcg cggccggcgg tggtcatatc gaatgatcgc ttcaatcgcg cattcgagtc
      961 gctcatcata gttgtgcaga tgacacggac aaatcggggc atcccgaccc agataccggt
     1021 cgatcctccg gagggagggc tcaccgcccc atcgttcatc atgtgcgagc aggtgcgagc
     1081 ggtgagcatc gccgggttcc gcgggcggcg tggtgcgatc agagacgaga cgatgatgaa
     1141 ggtcgaggtg atgctcccac atctgatcgc tcgccttccg gacaggagat agtcgagtga
     1201 gcaactggtc ggtgctgctc atcgccgggg cgtcggggac cggcaagacg accgccgaag
     1261 gcgatcgccc gccgccgcgg cgtcacctgg gtgcaggtcg acgacctccg gctcgcactc
     1321 cagtggagcg gtgcccgttt gccgagcgag gccgccaccg aggcgctcta ttacttcgcg
     1381 cggacgcccg gtgtctggag tcagccggca gaccgccttc gtgacgcgct catcgacgtc
     1441 ggccgcgcga tgacggacgc gatcgcgatc gtggtcggca accacgtcgc gcaaggcgac
     1501 acggtcgtca tcgagggtga cggcatcctg ccggagctgt tggagcacac ggaggtcagg
     1561 ccacatgccg cgtccggcgc ggcgcgaatg gcgctgcttg tcccggattc ggagcatgcc
     1621 ctgctgcgcg cgatgctcga ccggggccga ggcatcgcgg agattggcgc gacgcccgag
     1681 gcgcggaggg cggctgtggc gagttggtga ttcagccagt ggctccggcg ggaggcagag
     1741 gcgcgctcgg tcccggtggt tgcatccgat cccggggaga cgcttgcata tcggatcgag
     1801 aacgtcctga catgaagagg acgagtggtg actgctccct ttgaatcgtt cttccggagc
     1861 agggaacatg cacgcgacac gtacctctcg cggctcttcg cgttattcag tgaacaggtg
     1921 gtgcgagtct ggtgcatgtg cccgcaggcg ccgtacgagg atatcgggcg accgacgctc
     1981 tatgagccgg gggtgagccg cggaagcacc ctcgatttca cgctccgtga ccgggcgtcg
     2041 ggaagaacat tcgtctccga gttgaagtgt gagctggagt acgacaacta tcgctatctt
     2101 cggcttaccc acgccagcca actggcccat cccacctcca cggcgtttca gcggcttctc
     2161 cttgccgcca aggagccacc tgctctggg
//
LOCUS       DASYWL010000162         6482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_645464, whole genome shotgun sequence.
ACCESSION   DASYWL010000162 DASYWL010000000
VERSION     DASYWL010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6482
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645464"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /locus_tag="VGR22_05750"
     CDS             complement(<1..1172)
                     /locus_tag="VGR22_05750"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2128107.1"
                     /translation="MLLELAINDFAIIASSRIEFGPGMHALTGETGAGKSILLDALGL
                     VLGDRASTDAVRTGARRARIEALFDLADAPRDRIIAMLEEQGIEPDKEQLVITREVQA
                     GGRSVARINGQIVTVSVLNAIGGLLVDVHGQSDHFAIRRKDEQRRLLDRYAGLAPLVE
                     EVGELSGEVNQLRSRLASLSTGEREREQRRDLLAFQVEEIDAADLQPGEDGALANEQR
                     VLGNAEMLREETLHAIATLSGDDLGPGGVDVGSTLRQAETGLARIAEVDAAAGGLAER
                     LAEISILTEDLGRDLRHYLDTVEVDEERLAEVEERLELIRTLKRKYGSTIEDVLAYRD
                     EVGRELESLTGEEFNTEALEARLIEAEKRLGTVAVKLSQERRHAADRLSAEIEESIA"
     gene            complement(1274..3538)
                     /locus_tag="VGR22_05755"
     CDS             complement(1274..3538)
                     /locus_tag="VGR22_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEV2128108.1"
                     /translation="MATTYKTIGKRTKRIDSPPKLTGREKFAGDLRFPGMLRAKLIGS
                     DYGHAKIVSVDALAALAVPGVAAVLTHDDLPVVRSEEGASPISILADGEALYVGHPVA
                     MVLAETDQAARDAADLVTVEYEELEVIVDLEAAAAEDAPSVSHGAVGNFAEEAEMHNA
                     DAGSGQPAAEDLPPNVSNAVDFQRGNLEKGLAEADEIVEFDFTSDVVHQGYIEPQVAL
                     AVPELGRLTIYTSTQAAFHGRQETAESLGVDLDDIKIVPMPVGGGFGGKFVLIEPLVA
                     AAAKAVDRPVLLQFTREDDFLTGNPAPDCKIHVKLGAKRDGTLTALDAFLLFDCGAGS
                     GSPLSIAAILLGGYYRFPNLSIKGYEVQTHRPSSGSYRAPGAQQASFAIESAMDEMAH
                     KLELDPLEFRLQNCVVEGDERPNGAHWPRIGLKETLEALREHPIWKEREQRRASGHGI
                     GLAVGGWPGGIEPATAACRLDSDGKLTVMLGSVDLNGTNTTFGQVAAESLGVSSGEVR
                     VRTADTDSAPFAGGTGGSKVTYTVGAAVRKAADDAVEQIKQIAAEQLEASVEDLELVD
                     GFVRVRGVPESGISLEDVALASMKSGGHGPVLGRGASSISQIAPGFAAHLAEVAVDEV
                     TGKVKVVNYAAAQDVGFAINPAAVEGQIHGGVAQGIGWALYEGIHYGEDGSPDASSLL
                     DYTLPRAEMIPPIEVLLVEVESEHGPYGAKGVGEPPAIPGPAVIANAIRDGIGARITD
                     IPIRPAAVLEAIPE"
     gene            complement(3641..3943)
                     /locus_tag="VGR22_05760"
     CDS             complement(3641..3943)
                     /locus_tag="VGR22_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128109.1"
                     /translation="MTRYMVIHEMNKDSEEHTHPPTRLRDLARELGKDGSQPRWLATF
                     SPDLNDDRVVSLWDASNAEQVRKAIEEYGFLDHLTPKVFAVREWGPADVLAAGDED"
     gene            complement(3940..4527)
                     /locus_tag="VGR22_05765"
     CDS             complement(3940..4527)
                     /locus_tag="VGR22_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128110.1"
                     /translation="MSRYASGEPGNICLARTAAGGMAVVGNADADAFAADVVVIERAE
                     SRLEIARLVGTVAAGHEPAVEARIIGSYRISADGQPLGDLLDQSLDREPEGHCWGALG
                     LPHGLDTTVVPSARAGLTNDFAVARGSRGVSELERHPTLTIAPGERVTIGEHSGMVTA
                     IDRRAGTIEVTTGDAERVTVSIDEIRRSNAQEQAQ"
     gene            complement(4524..5561)
                     /locus_tag="VGR22_05770"
     CDS             complement(4524..5561)
                     /locus_tag="VGR22_05770"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HEV2128111.1"
                     /translation="MRTTKHRAVWDTWGNSTAAERLHRAVEQGPRHAYLLTGPHHIGK
                     SHVALRFAASLLCRSTERSGEFCGGCSTCRRVLKGVHPDVTRFDLEWQASQDEGGSKN
                     LSLNIKTVREIGRHVSLRPAESNWRVVVVDDVETMQEPAQEAFLKTLEEPPSYVVLLM
                     LATDAELLLPTILSRCAIVPMVPATDALVTEALLDAGANEADAERIAVAVRGRVGLGL
                     RAVEDASLLETLSEHVSEAVRWVGGDPYERMAEAYRLAEGFTSDREMVFDRVAAVEAA
                     WRELMLSAHGVETIARHPVHHVGRLRGPDEGVAALRSIDRCVRDLEANVRPRLAIGTM
                     VAAWPKLEAPS"
     gene            complement(5568..6320)
                     /locus_tag="VGR22_05775"
     CDS             complement(5568..6320)
                     /locus_tag="VGR22_05775"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HEV2128112.1"
                     /translation="MRIIRHTGYINSRKRRARWIAFLGFLILTSTLWIALNPSLLVPA
                     YIAMFVGFIIFNLGMQQVGKWSRNPRNDQFLDALLRDKLSDRYTLLHFAPMGKTKAEH
                     LLLHPGGLLVITLRELDGRIVERGRRWTRKAGGLRRLFSFSGPQLGNPSMETEEAIKE
                     VEAYLAEHHMEFDVNGSIVFFHPEVELDIQDPIHPVLLGDELPGFVKDLEPDPSISQA
                     ELQAFLEPLAEGEDVEVPRVSSRRRPVRRRAA"
BASE COUNT         1105 a         2231 c         2019 g         1127 t
ORIGIN      
        1 gcaatgctct cctcgatttc ggcggagagt cggtcggcag cgtgccggcg ctcctgagag
       61 agcttcacag cgaccgtgcc caagcgtttc tccgcctcga tgagacgagc ttccagcgct
      121 tccgtattga actcctcgcc cgtgagcgac tccaactcac ggccgacctc gtcacggtac
      181 gcgagcacgt cctcgatcgt gctgccgtac ttgcgcttca gcgtacggat caactccagc
      241 cgctcctcga cctctgccag ccgctcctcg tcgacctcga cggtatcgag atagtgccga
      301 agatcgcgcc cgaggtcttc agtcaggatc gaaatctcgg cgagacgctc ggcgaggccg
      361 ccggcagcgg catccacctc ggctatccga gccagtccgg tctccgcctg gcgcagcgtc
      421 gagcccacgt cgacaccgcc ggggccgaga tcgtcgcccg aaagcgtggc gatggcgtgc
      481 agggtttcct cgcggagcat ctcggcgttt ccgagcacac gctgctcgtt cgcgagtgcc
      541 ccgtcctcgc ccggttggag gtccgccgcg tcgatttcct cgacttggaa cgccagaagg
      601 tcgcgccgct gctcccgttc ccgctcgcct gtggagaggg acgcgaggcg cgaccggagc
      661 tggttgacct ccccactcaa ttccccgacc tcctcgacca acggcgcgag tcccgcatac
      721 cgatccagca ggcgtcgctg ctcgtccttg cgccggatag cgaagtgatc cgactgcccg
      781 tggacatcga caagcaaccc cccgatcgcg ttgagcacgc tcaccgtgac gatctgcccg
      841 ttgatccgcg cgaccgagcg gccgcccgcc tgcacctcgc gagtaataac gagttgttct
      901 ttgtctggct cgataccctg ttcctcaagc atcgcgatga tccggtcccg tggggcgtcg
      961 gcgaggtcga acagcgcctc gatacgagcc cggcgggcgc cggtacggac ggcatccgta
     1021 gacgcacggt caccgagcac gagcccgagc gcgtcgagca ggatcgactt gccggccccg
     1081 gtctcgccgg tcagggcgtg catgcccggc ccgaactcga tccggctcga cgcgatgatg
     1141 gcgaagtcgt tgatggcaag ttcgagcagc atgggcgttg caggatggtc cgctgggaac
     1201 gagagacgca gcgatggcgc ttgaatggta ccaccgctgc gtccctcggc tctgtacgtt
     1261 cgatgctgct cgcctactcg gggatcgcct ccagcacggc ggccggccgg atggggatgt
     1321 cggtgatccg cgcaccgatc ccgtcccgga tcgcattggc gatcacggca ggacccggga
     1381 tcgcgggcgg ttcgccgacg cccttcgcgc cgtacggccc atgctccgat tctacctcga
     1441 ccagaagcac ctcgatcggc gggatcattt ccgcgcgcgg cagcgtgtag tcgagaagtg
     1501 acgaggcatc gggcgagccg tcctcgccgt agtggattcc ctcgtagagc gcccagccga
     1561 taccctgggc tacgccgccg tgaatctggc cctcgacggc ggcaggattg atggcgaagc
     1621 cgacgtcctg cgcggccgcg tagttgacca ccttcacctt gccggtcacc tcgtcgaccg
     1681 caacctcggc gaggtgcgca gcgaaaccgg gagcgatctg gctgatcgac gatgcgccgc
     1741 ggccgagcac cggcccgtgc ccaccgctct tcatgctcgc caatgcgaca tcctcgagcg
     1801 agatgcctga ctccggcacg cccctgacgc ggacgaatcc gtcgaccagc tcgaggtcct
     1861 caaccgaggc ctcaagctgc tccgcggcga tctgcttgat ctgctcgacg gcatcgtcgg
     1921 ccgccttgcg aaccgctgcg ccaaccgtgt aggtgacctt ggagccaccg gtgccgccag
     1981 cgaagggggc cgaatcggtg tctgccgtgc gcactcgaac ctcaccggag gagacaccaa
     2041 gcgactcagc cgcaacctgg ccaaacgtcg tgttggtgcc attgaggtcg accgagccga
     2101 gcatgacggt gagcttgccg tccgagtcga ggcggcacgc cgccgtggcc ggctcgatac
     2161 cgcccggcca gccgccgacc gcgaggccga tgccatgacc gctcgctcga cgctgctcgc
     2221 gctccttcca gatcggatgc tcgcgcagcg cctccagcgt ctccttgagc ccgatgcgcg
     2281 gccagtgggc gccattcggg cgctcgtcgc cctcgacgac gcagttctgc aggcgaaact
     2341 ccagggggtc gagctcgagc ttgtgcgcca tctcgtccat cgccgattcg atcgcgaagc
     2401 tcgcctgttg cgcgcccggc gcccggtacg atccggagct cggccggtgc gtctgcacct
     2461 cgtagccttt gatactcagg ttggggaacc ggtagtagcc cccgagcagg atggcggcaa
     2521 tgctcaacgg agagcccgac cccgctccac agtcgaagag caggaacgca tcgagcgccg
     2581 taagtgtgcc gtcacgtttg gcgccaagct tgacgtggat cttgcagtcc ggtgccgggt
     2641 tgccggtgag gaagtcgtcc tctcgcgtga actgcagcag gaccgggcga tccacggcct
     2701 tggccgcagc cgcaaccaga ggctcgatca gcacgaactt tccgccgaag ccgccgccga
     2761 ccggcatcgg cacgatcttg atgtcgtcga gatcgacgcc gagcgactcc gccgtctcct
     2821 gtcgaccgtg gaacgccgcc tgggtgctgg tgtaaatggt gagacggccg agctccggga
     2881 ccgcaagggc aacctggggc tcaatgtagc cctggtggac gacgtcggag gtgaagtcga
     2941 actccacgat ctcgtcggcc tctgcgaggc ccttctcgag gtttccgcgc tggaagtcga
     3001 cggcgttcga gacgttcggc gggagatcct cggccgctgg ctgtccggac ccggcatccg
     3061 cgttgtgcat ctccgcctct tcggcgaagt tgccgacggc tccgtgactg accgagggtg
     3121 cgtcctcggc tgccgccgcc tccagatcga caatcacctc gagctcttca tactcgaccg
     3181 tgaccaggtc ggcggcatcc cgcgcggcct ggtccgtctc agccaatacc atcgccaccg
     3241 gatggccgac atagagggct tcgccgtcag cgaggatgga gatcggggat gccccttctt
     3301 cagagcgaac gacaggaagg tcgtcgtggg tcagcacggc cgccacgccg ggcaccgcca
     3361 gcgcggccaa tgcatcgaca gacacgatct tcgcatggcc gtagtcactt ccgatcagct
     3421 tcgcccggag catgcccgga aaccgaagat caccggcaaa tttctcgcga ccagtcagct
     3481 tcggagggga gtcaatgcgc ttcgttcgct tgccgatggt cttgtaggtc gtcgccatct
     3541 ggaactcctg ctgcgaatgc cgcactgtgt gcaacgtcgg ctcaaacact gtcgccagaa
     3601 tcccacatcc ccgacagact gaaaagggga tgcccggccg tcagtcctcg tcgccggcgg
     3661 caagaacgtc ggcaggcccc cactcgcgca cggcaaagac cttgggcgtg agatgatcca
     3721 gaaagccgta ctcctcgatc gcttttcgaa cctgctcggc atttgacgca tcccagaggg
     3781 agaccacgcg atcatcgttc aggtccggcg agaaggttgc gagccaccgt ggctgagacc
     3841 catccttgcc gagctcgcgc gcgagatctc gcaagcgcgt cggcgggtgc gtgtgctctt
     3901 ccgaatcctt gttcatctcg tggatcacca tgtatcgcgt cactgtgcct gctcctgagc
     3961 gtttgaccga cgtatttcat cgatggaaac ggttacacgt tcggcatcgc ccgtggtcac
     4021 ttcgatcgtc ccggctcgcc ggtcgatggc cgtcaccatc ccgctgtgct cgccgatcgt
     4081 cacgcgctcg cccggagcga tcgtcagcgt cgggtgacgt tcgagctcgc tcacacccct
     4141 gctccctcgc gctacggcaa agtcgttcgt gaggccagcg cgggcggacg gcacgacggt
     4201 ggtgtcgaga ccgtgcggca gaccgagcgc accccagcag tggccttccg gctctcgatc
     4261 gagtgattgg tcgagaaggt caccgagggg ctgcccatca gctgagatgc gataggagcc
     4321 gatgatcctc gcctcgacgg cgggctcgtg ccctgctgcc accgtgccca caagtcgcgc
     4381 gatctccagg cggctctcgg cgcgctcgat gaccacgacg tctgcggcga atgcatctgc
     4441 atctgcgttg ccgacgacag ccatcccccc ggcggccgtt ctggcaaggc aaatgtttcc
     4501 cggctccccg gaggcatatc ggctcacgac ggcgcctcca gctttggcca agccgccacc
     4561 atcgtcccaa tggcgagcct cgggcggacg ttcgcctcca ggtcgcgcac gcaacggtca
     4621 atggaccgga gcgctgccac gccctcgtcg ggacctcgca gcctgccaac atggtgcact
     4681 gggtgacgcg caatcgtttc gacgccatgc gccgaaagca tgagctctcg ccacgccgcc
     4741 tcgacagcag cgacccggtc gaacaccatt tctcggtcgg atgtgaatcc ctccgccagc
     4801 cggtacgcct cggccatgcg ctcataggga tccccgccaa cccaccgcac cgcctcggaa
     4861 acgtgctccg acagcgtctc cagcaacgat gcgtcctcca cggcccggag gcccaagcca
     4921 acccggcccc tcacagcgac ggcgatgcgc tcggcgtcgg cctcgttcgc cccagcatcc
     4981 agtaacgcct cggtgacgag cgcatcggtc gccggtacca tcggcacgat ggcgcagcga
     5041 ctgaggatgg tcggcagcaa cagttcggca tcggtcgcga gcattagcag cacgacatat
     5101 gagggaggtt cctccagcgt cttgaggaag gcttcctgcg ccggctcctg catcgtctcg
     5161 acgtcgtcca ccacgacaac acgccagttg ctctccgccg gtcgcagcga gacatgccgc
     5221 ccgatctcgc gaacggtctt gatgttcagc gagaggttct tcgagccgcc ctcgtcctga
     5281 ctcgcctgcc attccagatc gaagcgggtc acgtccgggt gcacaccctt cagcactcgt
     5341 cggcaggtcg agcagccgcc acaaaactcg cccgaacgct cggtgctacg gcacagcagc
     5401 gacgcagcga accggagcgc gacatgtgac ttgccgatat ggtgcggccc cgtgaggaga
     5461 taggcgtgcc gcggtccctg ctccacagcg cgatgaagcc gctcggccgc ggtcgagttt
     5521 ccccaggtgt cccagacggc tcgatgcttg gtcgtgcgca tgcggtgcta cgccgcgcgc
     5581 cgccgcaccg ggcggcgcct tgagctgact cttggcacct cgacgtcttc gccctccgcg
     5641 agcggctcga ggaaggcctg cagctcggcc tgcgagatgg acgggtccgg ctccaggtcc
     5701 ttgacgaagc caggcagctc atcaccaagg agcaccggat ggatgggatc ctgaatgtcc
     5761 agctcgacct cgggatggaa gaaaacaatc gatccgttga cgtcgaactc catatggtgc
     5821 tcggccaagt aggcttcaac ctccttgatc gcctcctcgg tctccatgct cgggttgccg
     5881 agctgcggtc cgctgaagga gaacagtcgt cgcagcccgc cggccttgcg ggtccaacgc
     5941 ctgccccgct cgacgatgcg cccgtcgagc tcgcgtagcg tgatgacgag caatccgccg
     6001 ggatggagca ggagatgctc cgccttggtt ttgcccatcg gggcgaagtg caacagcgta
     6061 tagcgatcac tgagcttgtc gcggagcagc gcatcgagga attggtcgtt gcgcgggttg
     6121 cgagaccact tcccgacctg ctgcatccct aggttgaaga tgatgaagcc cacgaacatc
     6181 gcgatgtagg ctggcaccag cagtgacgga ttgagcgcga tccagagcgt cgacgtgagg
     6241 atcaagaagc cgaggaacgc aatccacctc gcccggcgct ttcgcgagtt gatgtatccg
     6301 gtatgtcgaa tgatccgcat gcaggcttcc agatagtcag gcggcgatca ggccgcgcac
     6361 gaaaaaactc aaccctgcga gtttatgccg actctcgcac ctgccgcaaa tgccgcaacg
     6421 accgcatccg aatcggtcgt gttgcatcga tgttgcgaac gaaccgcaga tcgatgcgtc
     6481 ac
//
LOCUS       DASYWL010000163        37013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_25948, whole genome shotgun sequence.
ACCESSION   DASYWL010000163 DASYWL010000000
VERSION     DASYWL010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 37013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37013
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25948"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VGR22_05780"
     CDS             <1..344
                     /locus_tag="VGR22_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV2128113.1"
                     /translation="ARDEGMYSGATQRGSRGGRIPGAVNVPAQSFTGIDGCWKAGDEI
                     RQQAVEAGVALDLPVTAYCNGGVTATQLLFGLHRAGMPLDLLSNYDGSWNEWGERADL
                     PVEGNQDLFRD"
     gene            complement(598..673)
                     /locus_tag="VGR22_05785"
     tRNA            complement(598..673)
                     /locus_tag="VGR22_05785"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(638..640),aa:Phe,seq:gaa)
     gene            complement(710..782)
                     /locus_tag="VGR22_05790"
     tRNA            complement(710..782)
                     /locus_tag="VGR22_05790"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(747..749),aa:Val,seq:tac)
     gene            complement(811..898)
                     /locus_tag="VGR22_05795"
     tRNA            complement(811..898)
                     /locus_tag="VGR22_05795"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(862..864),aa:Leu,seq:gag)
     gene            1069..2127
                     /locus_tag="VGR22_05800"
     CDS             1069..2127
                     /locus_tag="VGR22_05800"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEV2128114.1"
                     /translation="MAPLPPESKLISTADSLLEETETPGAAVAILVDGERVFHAGVGS
                     LDLARGKAIEVDAQFLLYSVTKMLLAAVTMRLVERGIVSLTDSVASLIPEAAIDRRMS
                     LRHLLRHTSGLRDYGGMPEYHEAVRTSPMEPWPEATILGRTLAQGALFEPGQGWAYSN
                     IGYLLIRRVVGRHAGGSLATALDELLFRPLGLERTRMCETLDDTLELTPGFTTSIGEG
                     GLVDVRGRYHPGWVAHGVVQSTVAELAVVVDALLAGHLLGQQSLAVMRAALPVLVTHP
                     VIRVPGYGLGLMMDLDPGSHSLTGHAGGGPGYSAAAFSIRSGDGRRITIATLANRDAD
                     EVALRMVVELGGMLAGTS"
     gene            2279..3247
                     /gene="mdh"
                     /locus_tag="VGR22_05805"
     CDS             2279..3247
                     /gene="mdh"
                     /locus_tag="VGR22_05805"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEV2128115.1"
                     /translation="MATDVARRTGRSKVTVVGAGMVGGTVAQLVAQGDYADVVLVDIV
                     EGMPQGKALDLMQAGPVLGYDSRLTGTNGYRETGNSDVVVITSGIARKPGMSRDDLLK
                     TNMGIVESVTKDIVATSPNAVIIVVSNPLDAMCHVAFDTAGFPRERVIGQAGVLDSAR
                     FRTFIAMELDVSVEDVSAFVLGGHGDTMVPLSRYSTVAGIPITELIPADRLREIEQRT
                     ANGGAEIVSLLKSGSAYYAPASSVVEMVQSVLLDKKRILPSSVLLQGEYGISGLYVGV
                     PAKLGANGMEQVIEISLTDEEQAALQNSANAVQGLVEAMERLRNEA"
     gene            3438..4409
                     /locus_tag="VGR22_05810"
     CDS             3438..4409
                     /locus_tag="VGR22_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128116.1"
                     /translation="MPKILGALGTVRGFMNVVKEVDFDEVRSRAETAPRVLVVADEQG
                     RAERFVTEVFGDDAMRHVDIRTGWFEDIDASRWDVIVVLDPTDSGVLPKVKSAVGEAA
                     KRKLVDAKVCGPGMTVEEITAAQDAIVSADTEFAAALGRRFPELQLAAVKAIVDETAK
                     ANAQFALVSNAPAIVPFIGGIVAASADLIVLTKNQVMMVYKIAAVHNRALDNQLKLMR
                     ELAPVVGSGFLWRTLAREAASFLPLAAGTVPKVAIAYVGTVVMGRAADYYYRFGKKPT
                     REHLREFTRRASETASRLQISRKDEQEARKAEPAETAERQQPTAEVS"
     gene            complement(4468..5835)
                     /locus_tag="VGR22_05815"
     CDS             complement(4468..5835)
                     /locus_tag="VGR22_05815"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEV2128117.1"
                     /translation="MSTIMIVDDDAAIRGVLRDLLEDEGFEVAEAETGRAVIDSLSGD
                     TPPDLIMMDVRMPDKSGIDVLREVKPTNNGPLPIIMMTAFGTSSVAIQAMQGGAYDYL
                     TKPFDLDDVMLTIRRFFDRKQLSDQIEELSKRLDKPDPNDLMIGNSPAMQEVYKTVGR
                     VARSDATVLITGETGTGKELIATILHETSNYARGPLVKVNCAALPETLLESELFGHEK
                     GAFTGAMNQRKGRFELANKGSIFLDEIGEMTLSTQKKLLRVLQEREFERVGGTVSVKV
                     DTRVIAATNKVLQQEVEANRFREDLYYRLNVIAIYLPPLRERREDIPLLVEHFLSKYR
                     ATPRSEPSRMSQQAVEMLMDYDWPGNVRELQNLVERAVVLSQGSIITPEHISFFGADS
                     RRLFDIGTLVRRGTSLDDLLDEVEHAALEEALAAAKGDRSEAAALLGLKVPQFNKRIS
                     ALSPG"
     gene            5964..7613
                     /gene="aroB"
                     /locus_tag="VGR22_05820"
     CDS             5964..7613
                     /gene="aroB"
                     /locus_tag="VGR22_05820"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEV2128118.1"
                     /translation="MTFRRVVLIGMSGAGKSTVGRMLAGRLGWRLIDLDAEIERRQGR
                     SIPDIFRTSGEDVFRAIERQEFRAALESENAVISTGGGAVTNEEAWGSDLLGASGTLS
                     VLLDAPAEVLYQRLVEQQAQDPSGAIRPMLSGSNPLARIRALLSERDRWYRRAGIVLP
                     VGDRTVDEVTGLVADALGRRSWLVGLDVSGAASRIYVGADVLDFLGVRISEQWPKARR
                     VWIVADEDVAGYHLQATVSAAERDGAEVRTLTFPSGEASKSLAGLGGLYDGLLRGGIE
                     RSDVIVALGGGVAGDLVGFAAATVLRGGGLVQVPTTLLAMVDSSVGGKTGINHPAGKN
                     LIGAFYQSPVVLIAPEMLKTLPNREYRSGWAEIIKHGLIEPTTPAGDSGLFDLVVANA
                     RLLNDRASPLLNPIIARNVEIKASVVQADEREAGLRAILNFGHTIGHAIEAAGYTLLH
                     GEAIAVGLHGAMRFGEALGRVSSDRVDEVVSVLEAFGLPTTVDIDSGEIRRRMQSDKK
                     RVAGRQQWIVPLPEGGVSVERGVPETAVDAALAAIIARDSA"
     gene            7756..8751
                     /locus_tag="VGR22_05825"
     CDS             7756..8751
                     /locus_tag="VGR22_05825"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEV2128119.1"
                     /translation="MTQLPNIVVSASIAFDHIMSFNGSFKDHILVEKAHVLSVSFLID
                     SLKKHRGGVGGNVAYSLGLLGEPAALVGTVGEDFGEYRSVLDQHGVDTSGVVEVGGEL
                     TATGFLSADLHGNQIVSFYPGAMWKSREIDLTDAANGAVYGVVGAGDPEGMVLHTRQI
                     AASSAKLVFDPSQQIPILTGDQLTEGIEAADIMVANDYEYSMIERKTGLSIEDIRQRV
                     ELTVVTYGEQGSEIRHGDEQVEIPIAPAREVVDPSGGGDAYRAGLLKGLLLGFDLDMV
                     GRVAAQAATYPIENYGTQEHVYDVPGFIARFEESFPEYAGRLHESDMVAAIADRS"
     gene            8795..10069
                     /gene="ahcY"
                     /locus_tag="VGR22_05830"
     CDS             8795..10069
                     /gene="ahcY"
                     /locus_tag="VGR22_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEV2128120.1"
                     /translation="MATPSTDTRNFDIKDPSLAPGGRRRIDWAAREMPVLARIRERFA
                     KEQPLKGLRLVACAHITTETANLALTLQAGGAEAVLCASNPLSTQDDVAATLVEEGIA
                     VFAIKGEDAETYDRHIHSAIAHKPHIVIDDGADVIATIHRHYAEMLPDIKGGTEETTT
                     GVIRERAMEKAGVLGFPIVAVNDADTKHFFDNRYGTGQSTIDGILRATNILLAGKTVV
                     VGGYGWCGKGIAMRMRGHGAQVIVTEIDPTRALEAALDGFRVMTMEQAAPVADFIVTA
                     TGNIDVVDRRHFEVMKDGAVLANSGHFNSEINLKALGEMAGEGRRQLRPFVEEYSFNG
                     KRVMVLGEGRLINLAAAEGHPASVMDMSFANQAMAAEYIAKEGEHLENKVYSVPEAID
                     MEIAALKLNAMGVEIDTLTPAQEKYLNSWETE"
     gene            10130..11359
                     /gene="metK"
                     /locus_tag="VGR22_05835"
     CDS             10130..11359
                     /gene="metK"
                     /locus_tag="VGR22_05835"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEV2128121.1"
                     /translation="MSTTFDTAERVLFTSESVTEGHPDKICDQISDAVLDAALMDDPS
                     SRVACEASTTRGLVFVFGEMTTETYIDIQEVVRQVLTDIGYTHSSHGLDADTCGVFVS
                     LREQSPEISAGVTTALESRSGSSADEADLTGAGDQGMMIGYACRETPELMPLPISLAH
                     KITRRLTETRKSGQLAYLLPDGKSQVTVEYSHGRPVRVDTVVVSTQHKPDVPNAQIER
                     DVVERVVKAVVPADLLDTNTRYLVNPSGRFEIGGPAADAGLTGRKIIVDTYGGMARHG
                     GGAFSGKDATKVDRSAAYAARYVAKNIVAAGLADRFELQISYAIGKAQPVSIMCETFG
                     TGRIADDQIETLVREHFDLRPGAIIRSLDLRRPIYRQTAAYGHFGRPDLDLPWERTDK
                     ADALASAAGFAQAATAD"
     gene            11437..11796
                     /locus_tag="VGR22_05840"
     CDS             11437..11796
                     /locus_tag="VGR22_05840"
                     /inference="COORDINATES: protein motif:HMM:NF017920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEV2128122.1"
                     /translation="MKFIIAVIQAYDTDRLLRAVTSAGLRGTKIVSTGGFLRMANATI
                     FMGVDDARVEECLQIIREHCESRIESSIDPGEQEYIEWFPIGVHEVTVGGAVVFIVGV
                     NSFHQMFPPSPASERQR"
     gene            complement(12053..12340)
                     /locus_tag="VGR22_05845"
     CDS             complement(12053..12340)
                     /locus_tag="VGR22_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128123.1"
                     /translation="MNSSMIGKIEKAHRYAREPERITFETFTASFRGSHDNYAVSLTE
                     KGWECTCHTFETHMLGTCSHIMAIQHILGRMLPDRARYDSEEIESLQAVVS"
     gene            12734..13093
                     /locus_tag="VGR22_05850"
     CDS             12734..13093
                     /locus_tag="VGR22_05850"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HEV2128124.1"
                     /translation="MSANMDSLSILQRAQLAWNLMRDQRVAGWVKKAGPAVILVYVIS
                     PIDVIPDFLLGAGQVDDVGVIALGLLLLVRLLVRFAPQEVVDEHVARITGVPGANRQS
                     RRSRTDGPTIDTSGRVR"
     gene            13288..13416
                     /locus_tag="VGR22_05855"
     CDS             13288..13416
                     /locus_tag="VGR22_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128125.1"
                     /translation="MPTTQLEPETEYGIVRRDLRRLTIFSVICFALMIAVLFFLNV"
     gene            complement(13519..14382)
                     /locus_tag="VGR22_05860"
     CDS             complement(13519..14382)
                     /locus_tag="VGR22_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128126.1"
                     /translation="MTDTQEVPQQQPNKRQMVEQARDIALAGDWEQALEINASFLQRF
                     PNDAEALNRKGRALLELGRLQDAWDAYSEALAADPANMIARRNLQRLEMLVNTDVSDR
                     VSDEQIGSPRANVFIEEVGKTWVDELINSTEEAVLATVSPGEQLTLQVENSRIVVSSR
                     TGERLGELEQRISRRLEELIAAGNRYEIYALGMSGHSLRIIIRESYQDPSMEGQPGLP
                     RQSRQTIDLMRERELLSLREEGDFSFGEEDEEELVDEEVGDEEDEVEVDEEAAQYVDS
                     SLAGDTDDDAM"
     gene            complement(14432..15796)
                     /gene="miaB"
                     /locus_tag="VGR22_05865"
     CDS             complement(14432..15796)
                     /gene="miaB"
                     /locus_tag="VGR22_05865"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEV2128127.1"
                     /translation="MSTSPLIQIQPKTSPARLPRNGKRFYIWTIGCQMNEADSAKVAA
                     MLQEVGYEPTRSEDDADIVVLNSCVVRQAAEDKVSGKLNSLVRLKRNRPEVPVVLTGC
                     MVTKQQEKLREAFPHVDLFFDPSDFDKLEQVVPELSELEDDLQQLPHYYLPQQHTGTS
                     AVTAYIPIIYGCNFLCSYCIVPYRRGKETSRPLKEIVREVERVVETGVREVTLLGQTV
                     NAYGHDFPEPQDLAQLLRAVDKVPGLERLRYLTSHPKYMTDELIDTMAELGTICEHMN
                     LPVQAGDNEVLRRMRRTYTRDLWMERIAYTRERMPGVTVATDVIVGFPAETEAQFMQT
                     FDMLEQTKVDKVHLAMYSPRPGTLSARWDDDIPHEEKLRRHQMLEKQQERIATDINSR
                     RLGETHEVLVEGKSRGRWTGRTRANTLVHFDDDRELTGNLVDVQITRTSPWFLMGEPV
                     GAPR"
     gene            16046..17332
                     /locus_tag="VGR22_05870"
     CDS             16046..17332
                     /locus_tag="VGR22_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128128.1"
                     /translation="MFVRTLSRSWGPLLVASLAVVAMLNTSMAAAPFSGAPQARLAQV
                     NVEWMSEEQAAALNLSFPVLVPSWVPAPFAGAPSVQAGGGYYSLYWMVPGGDPTFLQV
                     AGEVGGAMPVGSPADLNVRLEINASVNGYDAIHDVTAIYDNVWWVQGGVLYTVSSKNM
                     TGTDSLALANSLVQLQPPVQPPTSPPPTPTPEPVESSPSGAIYNAAEVPSANIATIEA
                     WPSATSTLRATHGSFSASGASFLSVDGGIFSWQAPVVDRNTTVEFTLIDDATGEVTAW
                     SSIVVYPTSTGSSTLPVQEPITEPETGVSSGPTSMPADDALPSSITSTSTAAVSESAS
                     QPTTTITTSATDEDEGDTSASSSTGDATTSVEADTGTSETVTRDETPEAGGAGGGLTS
                     DGTAGPPMPRTGDGTSGPSLPAGGDGTGGIRQIAVP"
     gene            complement(17382..19586)
                     /locus_tag="VGR22_05875"
     CDS             complement(17382..19586)
                     /locus_tag="VGR22_05875"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2128129.1"
                     /translation="MRQDELEPTEEPRESRVAGWARARMAPETWDRITRAAHSTTERM
                     HQSSESLRRWASIGSDQVTSMRQARVELWRLVFAVGTIAVPLLLISLTAPEVDAALVA
                     PLLVVSVMLATYVADWVGGVTATIAGAVLLDVTLLGERWLLDAWNEPRGIVVLLLFLI
                     LAGAVAATVERLKYDQASARLDAAAMRAANTALNAVEIAAAQRPAGDNAAYISVFENI
                     LTAMVRVNRASAGAIYLTDGPSNALVQAVTYGSQEETAHQMRPSVEIPFGDGFVGRVA
                     RERRPLAINDTSTVMPEEIGDVLEVNRHVRSVLGVPLFGAEERVVGVAWVGLYAPYRF
                     AQTATARLEALAHRAVAFMESASIADAQEELLGRISDSHRRLQAVIQTMPEAVMVVRP
                     PQGIIVAQNAAATRMFRLPATGNPFSLRVDQLRLDGDYAVEDLPINRALTSGETVTGV
                     ELAVHQAGGDPLPVIGSAAPLWADDGTVDAVVGVFQDVRPLKEAERMRDEFVSVVSHE
                     LRSPLTPIRGFAQVVARDLAAEGGHAQHIAYLETLQRHADRMTRLVDDLLDVSRARAG
                     RLTIRRSPADVVDIARRVVDSANASTPAHTVRLETPWSGHESNVDEDRVHQIIDNLVG
                     NAIKYTEGGAITVGVGPSTREGWGLEITVTDEGKGIPEAEREQLFSAFYRRREASEGA
                     MPGLGLGLYICSELVRAHDGRITIEDAPTAGTRFVVLLEPNNQRMSARKTAA"
     gene            complement(19611..20843)
                     /locus_tag="VGR22_05880"
     CDS             complement(19611..20843)
                     /locus_tag="VGR22_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEV2128130.1"
                     /translation="MATTQAVPLESLISRRAKDLPGLRVGDPRKSAEIISFYAGFPDR
                     DSLPKTSVIEATKTALEAEGEWALQYGHGLGNRNLAQILLDKLRRDFGIEAEQDQILI
                     TAGASQAIGLAIEALVDWGDTIITEAPTWMGAVRMFRNAGAQPVNAPVDAAGTNVEAL
                     EKTLADLKANGITAKFIYTIPNFQNPTGVSTSPERRERIVELAHEYGTFILEDDAYAD
                     LRYSGEQIPPIFSLDNRGTTLYFGTLSKTLGAGMRLGWVVAHPALVSKMGVLKVDGST
                     NVFGSYVAAEWIPDHLDKHIEELKAIYQHRRDLMLAELQKHFPEGCSWTEPEGGFFIW
                     VTLPKGVDTTRMAAQARERGVEYLPGRSCFTDGSGQNMLRLSYSFARDEEIGEGIRIL
                     GEIIDGEMAETGQRQASQ"
     gene            21085..22152
                     /locus_tag="VGR22_05885"
     CDS             21085..22152
                     /locus_tag="VGR22_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV2128131.1"
                     /translation="MVSNVTPADTRLEAKGDEDRMFDITVIGAGPTGLFAAFYAGLRG
                     ASVQIIDSLEEPGGALTAIYPEKYIYDVIGFPKVLAKDFVGACLEQAMRSGPTIRLNE
                     QVQDLLPQEDGTLILRTSKAERRSRSVIVAAGVGAFEPTRLTAPGVRELEGKGVHYFA
                     KRVDDFRDKNVVIVGGGDSAVDWAVTLEPIAKTIKLIHRSKFRAHEATVEELERSSVE
                     LFYPKCEVTEVWPGPDGRIGSLTFKNGDDELIDLEVDELICAIGFKADPGPMRSWGFE
                     LARNQIVVDKITMETNIPGVFGAGDIVSYPAKFKLIANGAAEAVTAINHAVTYFNPDA
                     RLDPGHSTTIMEKRAKETAAD"
     gene            complement(22266..23318)
                     /gene="trpS"
                     /locus_tag="VGR22_05890"
     CDS             complement(22266..23318)
                     /gene="trpS"
                     /locus_tag="VGR22_05890"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEV2128132.1"
                     /translation="MVTHAPDRASNQADTAEELKKVRVFSGIQPSGNFHLGNYLGAIR
                     NWVNQIENSDSIFCVVDLHALSNNPDPDVLQQQIRNVASSLLASGLDPQKCLLFVQSD
                     VPAHAELQWLLNSVTQFGELRRMTQFKDKSAGKGDSVSAVVFSYPVLMAADIVLYDAE
                     EVPVGDDQRQHVELTRDIVQRFNARYGETFVVPKADIKEEAARIMALDKPDEKMSKSS
                     PRENSYVAFSDPPDVIRKKIRRAVTDSGETVTAGPDKPALTNLIQIYGSLTGMTPSQV
                     EAHFEGKRYGDFKKELADVVVDRLAPIQAELTRLNDDAGFVAGVLQRGAEQANDMASG
                     KMGLVRERMGIGRPRP"
     gene            complement(23427..23798)
                     /locus_tag="VGR22_05895"
     CDS             complement(23427..23798)
                     /locus_tag="VGR22_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2128133.1"
                     /translation="MVSNQARLVSCPVARTADLIGNRWTPLILRDLAVGQRRFSQLER
                     SLSGISPKTLSERLKRLEDAQVVTRRCYAQVPPRVEYALTEKGKALLPVIDMMREFGA
                     AWLPEECSGEAVRTPETASAR"
     gene            23994..24338
                     /locus_tag="VGR22_05900"
     CDS             23994..24338
                     /locus_tag="VGR22_05900"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEV2128134.1"
                     /translation="MVRFIAYVVATVVATLALGTISERLIEFDRAESVLLFAVVIGAI
                     NAFIKPIVNLLTLPITCLTFGLFTIVVNMLLFWIGARITPNVEIPTWWGALFGSLLVS
                     VAAGIIFTVIDE"
     gene            24331..24954
                     /locus_tag="VGR22_05905"
     CDS             24331..24954
                     /locus_tag="VGR22_05905"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV2128135.1"
                     /translation="MSRYPIEDELVTERRHFGDPLTIGVMSDTHIYSRGRRVVPPGVI
                     RLFRRARIDLLVHLGDVNSRHVLEELGELAPLIGVPGNNDEPELHFMLPETTQFAIGE
                     RTFGVLHGHGGCTARDEAIRRFVGKVDCVLFGHSHKPLIDQIDETILFNPGSPTDRRW
                     NEHFGVGIIRVEAGTIRPELMLFTDADHLDNIDVADIAGEGRLGDDE"
     gene            24951..26063
                     /locus_tag="VGR22_05910"
     CDS             24951..26063
                     /locus_tag="VGR22_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEV2128136.1"
                     /translation="MTNHIQDPRLTDGRWLRIPGPTPVPPPVLAAMAEPMIPHRGKEM
                     SALIRRLRERLQEVHRTEGDVMVWSGSGSAGWEAALQNSCRPGERVVATVTGDFGRRF
                     ADVAERLGLWVTRVEAPWGQAVSPQQVEDAIKRAGGVRAALITHNETSTGVTNPLREL
                     AKVARRHGALVLVDAVSSAAAMPVEVGAWDLDWVISGSQKAWMCPPGLMIAALSERAL
                     QAAADQSNRRFFWDVEKMASAFQNGHATTTPAIPLYRALNVALDMMLDEGVEAMWERQ
                     AALGAWLRDRLKGLGLVLYADEAVASASVTAVRPPAGMPASTLRDMILADSGIEVAVG
                     QGPETEAVIRVGHMGWSHRPEMEATVESIARVLSHR"
     gene            26194..28134
                     /gene="rpsA"
                     /locus_tag="VGR22_05915"
     CDS             26194..28134
                     /gene="rpsA"
                     /locus_tag="VGR22_05915"
                     /inference="COORDINATES: protein motif:HMM:NF005208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEV2128137.1"
                     /translation="MDGTDVREQGGVNGDMQETQVDSQSAAGTDYEQELDGASLMEQF
                     LSDPDHDYKTLKYGDVLEGIVMHKDRDELLVDIGSKAEGIVPAKEYSSLTDEEKENLA
                     VGDTVLVFVVQPENQEGHPVVSIDRARQEKSWRQLQERHEANDVIEAEVTNYNKGGLL
                     VNLDGVRGFVPASQVTEIRGGDEASKQADMARLIGTSLALKVIEINRHRNRLILSERQ
                     AVQERRDAMKEQLIKELTEGEVRTGTVSSLCDFGAFVDIGGADGLVHLSELSWSRVRH
                     PSEVLNIGEEVQVYVLGINAEEKKIALSIKRTQPEPWSRVATSYEVGQLVKGTVTQLA
                     NFGAFARIEDGIEGLIHVSELAEERIQHPKQVVEEGEELILRIIRIDPQRRRMGLSLR
                     RALETPDSELASVFGDDILAERDNLARKVRSRLEAEGLVGQAPQPTPEQAAAASPEAL
                     EDLDEEAAKRAAGEAQVAAFEERRRRAEEAEQARQRRQEERRQQRGASSGEFAGGDMS
                     AMAMAFAQAGASDLNDLISGEEQEAGESKSGQSPSAAADSAGENAQPAQVAGAAGEAR
                     EDTNAEGDAALADETTAQRLAGDEQIENPVSEDEKITDAGSEWDKTIVEKGSADEGDV
                     VPNPETEETENPQPGEQPKGQE"
     gene            28344..30812
                     /locus_tag="VGR22_05920"
     CDS             28344..30812
                     /locus_tag="VGR22_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV2128138.1"
                     /translation="MAEKFDKFTDRARKVLTLAQEEAQRFNHNYIGTEHLLLGLVREG
                     DGVAAKVLNNMGVQLPKVRSAVEFIIGRGEGMIMGEIGLTPRAKKVIELSVDEARRLN
                     HHYIGTEHLLLGLVREGEGIAAGVLESLGVNLEKVRSEVMQVVSQSTSGSGQQQGSKQ
                     QTKTPYMDALGFDLTEAARTDRLGPLVGRSVEIDRVMQILSRRTKNNPALIGEPGVGK
                     TAIVEGLAQRIVSGDVPEQLQNKRLVALDIGALVAGTKYRGEFEERLKKIVNEVKETK
                     AILFIDELHTLVGAGAAEGAVDAANILKPALSRGELQTIGATTLDEYRKYIERDAALE
                     RRFQPVQVNEPSIEETIEILRGVRALYETHHDLKISDDALGAAANLAARYVTDRFMPD
                     KAIDLIDEAGSRVRMQRSAAPPSLKEAKAGLTAIQSELGAAVDGRDFELAAELRDRER
                     KLRDRIEEQEQKLADEKQSDEAYVTEEDVAQVVSMWTGIPLVRIAGEESERLLHMEDA
                     LHERIIGQDEAISMISKAVRRARAGIKDPHRPIGSFIFLGPTGIGKTLLARALAEFMF
                     GSEDHLIKIDMSEFMERHSVARLVGAPPGYVGYEEGGQLTEAVRRKSYSVILLDEIEK
                     AHPEAFNMLLQIMEDGSLADAKGRRVDFRNTILIMTSNVGAEEISRDLTLGFAIQQDE
                     EISRRREFDTMREKVMSRLKQTFRPEFLNRLDATIVFHSLSKEQIRQVVEVELARVRK
                     QLGEQEITLDFTVEAMDLIGERGYDHHYGARPLRRVIQNLIEDELAEGLLNGRFMTGQ
                     SIRVDVSKDGEALELTPVEELAPV"
     gene            30837..32315
                     /gene="radA"
                     /locus_tag="VGR22_05925"
     CDS             30837..32315
                     /gene="radA"
                     /locus_tag="VGR22_05925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEV2128139.1"
                     /translation="MGAAPVRWLRYHRPALRSYLEESTGVPKQRTSWICQECGTSSPA
                     YLGRCPGCGAWHSMVETLDQPAPAARMRSTARQDGSRPVRLHEVDVSTGGRIALRIDE
                     FNRVLGGGIVPGSVVLIGGDPGIGKSTLILQAGSDLSSRGMPVLYVSAEESAQQVRLR
                     ADRMGVDGKDLYIHAEIDLDSILAAAEQLKPGLVIIDSVQTIQSAELESTPGTVSQVR
                     TCASRLANWAKPNNVPVFLIGHVTKEGTVAGPRVLEHMVDAVLYLEGDRFHQYRVLRA
                     AKNRFGSTNEIGVFDMADSGLQEVRNPSEVFLEERNMMAAGSAVAVTLEGSRPILLEI
                     QALAAQTSFGLPRRSATGFDTNRLHLLLAVLQRRVGMGLGNHDVYVNVIGGFRVNEPA
                     ADLAAAVAIASGVRDTRVPADTVYIGEVGLSGDLRTVQHIDRRVQEAVRLGFRRAVVP
                     GRWKEAGQKAGGIEVTGMRTLRETVMDIPGMFGGRDLDAEND"
     gene            complement(32312..33463)
                     /gene="murG"
                     /locus_tag="VGR22_05930"
     CDS             complement(32312..33463)
                     /gene="murG"
                     /locus_tag="VGR22_05930"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEV2128140.1"
                     /translation="MIDADRPKERPLRLVIAGGGTGGHVLPAVAVVEELRRRMVPLEL
                     LWIGGHTGVERELAEANDIAFVAIQTGKLRRYLAIQTATDMLRIPLGIAQAFQALRAF
                     RPDVIFGTGGNISFPTVFAGSRMAPIITHEQTAQIGVANRMASRFADVFAVPFDETAV
                     LARQRHNRVVVTGNPVRSSILGGSREAGLRRFGFSPDFPVLYVTGGARGASPLNERVE
                     ALLPGLLEHAQILHQAGPPSANPDVERLQKLRTSWPDALQHRYHVTEFIRDEIADVYA
                     MASLVLGRAGAGTITELARVGKPSVLIPLPGTWGDEQTKNARMLSNAGAAEVIAQKDA
                     APTRLGEVITSLLADPDRLARMGEAATTVGNPQAASRLVDQILALAESS"
     gene            33569..34105
                     /gene="lspA"
                     /locus_tag="VGR22_05935"
     CDS             33569..34105
                     /gene="lspA"
                     /locus_tag="VGR22_05935"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEV2128141.1"
                     /translation="MRFAPPMTLPDLLARVAAVMAVVVVLDLLVKWAVTLWIGPDADR
                     HSWWLIGDSIGFEYLRNTGAAFGLFRGNPELLAAFTLLVTVGIVRLVLLEVRSPFWAA
                     LSSGLLAGGAIGNFVERVISGYVTDFVAVGPWPRFNIADSTITIGIAIFALAVILPDK
                     FGASDQATAEEGVRDRRA"
     gene            34089..35066
                     /locus_tag="VGR22_05940"
     CDS             34089..35066
                     /locus_tag="VGR22_05940"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV2128142.1"
                     /translation="MTDEPEINDSADMAYFELSPDISQRNERLDRYIASELPNLSRSG
                     VQALIEAGHVLVDCQRRKASFKVTPGEVVAVSVPPLAPDDVEPEDIPLEILYEDADVI
                     VINKAAGMVVHPAPGHASGTLVNALRFHAIEIAELDSSRTGIVHRLDKDTSGVMIVGK
                     HNRAVQFLQDQWLDGSVDKRYVAIAFRAFAEEEAVIDVPIARHRTERKRMDAGREGRE
                     ALTIASVRERFPKATLLDVDLQTGRTHQIRVHLAFIKHPVLGDTVYGTQSSTAYSREL
                     GARRQMLHAASLTVELPVGGGPRTFTASLPFDIRGVLDKLRAMKEQDDG"
     gene            35059..36363
                     /locus_tag="VGR22_05945"
     CDS             35059..36363
                     /locus_tag="VGR22_05945"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HEV2128143.1"
                     /translation="MVDLSDRSACRSLVERAYSAGIARVAPDEAVRRVLRRDGAALVV
                     DGERIPVSGRLVVAAIGKAATPMATAAAETAGDLIDQGFLLTKEGHAEQPPAGLEVFE
                     ASHPVPDERGIEASRRILEAVGGLGADDVVVALISGGGSALFEVPADGLTLDDIQQST
                     SILLRAGAPIQDLNAVRQELSRVKGGGFRRAIGDACTISLILSDVLGNPPEIIASGPT
                     VPRTTNPKAGLDVLDRYGVRNAVPSAVVRHLERTGERPDAPQAGKRGLYRVVGDNEAF
                     VAAVEDFFAQEGLTTERVWHEREGEAREQAHAWVEALESSAADVILGGGEMTVTVRGD
                     GAGGRNTEFALAAAINLEAKRLTATVASLASDGQDGSIDAAGGIVDELTVRELREADI
                     EPADAMERSDSGGALAAIGALLVPGPTGTNVNDVYIGLRHAI"
     gene            36449..>37013
                     /locus_tag="VGR22_05950"
     CDS             36449..>37013
                     /locus_tag="VGR22_05950"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEV2128144.1"
                     /translation="MFGLPGDTGMAFYDALYHEPAITHVMTRDERSASFMADVYARVS
                     YRLGICEGPSGGGATYSVPGVAEAHGSAVPVLCLTSDTPVSQQHWNVLTELDQVALFR
                     PVTGWTERVLAADSMDDALRRAIRMAISGRPGASHLSMPEDSLENDVLERVARQSDER
                     LTDRFPLSRPRADRAEVDRSARLLVEAE"
BASE COUNT         6675 a        11214 c        12483 g         6641 t
ORIGIN      
        1 acgctcgtga cgaggggatg tattccgggg cgacgcagcg tggctcccgc ggcgggcgga
       61 ttccaggtgc agtgaacgtg ccggcgcagt cgttcactgg catcgacggc tgttggaagg
      121 caggcgatga gatccggcag caggctgtgg aggccggagt tgcgctggac ctgccggtaa
      181 ccgcctactg caacggaggc gtgacagcga cgcaactgct cttcgggttg caccgggcgg
      241 ggatgccgct cgacctgctg agcaactatg acgggtcatg gaacgagtgg ggcgagcgtg
      301 cggacttgcc ggtggagggc aaccaggatt tgttcaggga ctgagaccgc ggtgcacccc
      361 gcctcagcac tctgcaactg acgcctcgag ccgtgccaga gtgcagggga cgcgaagagg
      421 agcactttct ctcatcggaa ggaggcgcgg aacgggcaag acatgtcttg acgcgggcct
      481 cactgtgacc gggtcaaggt cgatctgtga cctgcgcgaa cattcgcagg ctgcaagaaa
      541 caatcagcgc aacaagaaac cgggcgggga ttgatccccg cccggtttcc acctctttgg
      601 tgccaagggg cagactcgaa ctgccgaccg cacgattttc agtcgtgagc tctaccaact
      661 gagctacctt ggcaagttgt ctggccgatc ggctgcccga ccgttacggt gggcgataga
      721 ggactcgaac ctctgacatc cacggtgtaa acgtggcgct cttccagctg agctaatcgc
      781 ccctcagctg tcggcaatcc ggaccgatcg tggtgcccag gaaaggattt gaaccttcac
      841 gccctaaacg ggcactagcc cctcaagcta gcgcgtctgc ctattccgcc acctgggcag
      901 gtgcttgccg cctgacgatg cagcgacaag gattataggc gctgctggcg accgtcgtca
      961 actcgttgcg ccctcgtatc gtagtggata tacgccgcat cccagagcgc accaacgccc
     1021 ggatcaggcc gcggcgcaaa gagaagaaca taaagggaag aacgcgacat ggcgccatta
     1081 ccgcccgagt ccaaactgat ctcgactgcc gactccctgc tggaggagac cgagacgcct
     1141 ggcgccgctg tcgcgatcct ggtcgatggc gagcgcgtct tccacgccgg tgtcggtagc
     1201 ctcgatcttg cccgaggcaa ggcgatcgag gtggacgcgc agtttctgct gtacagcgtg
     1261 accaagatgc tgctggcagc ggtgacaatg cgcttggtcg agcgcggtat cgtgtcgttg
     1321 accgattccg tggccagcct gatcccggag gcggccatcg accggcgcat gagcctccgg
     1381 catctactcc gccataccag cggcctgcgg gactatggcg ggatgccaga gtatcacgag
     1441 gcggtgagga cctcgccgat ggagccgtgg ccggaagcga ccatccttgg gcgcacacta
     1501 gcgcaagggg cactctttga accgggacag ggctgggcgt actccaatat cggatacctg
     1561 ctgatccggc gtgtcgttgg ccggcatgct ggcggctccc tggcaaccgc gctcgatgag
     1621 ttgcttttcc ggccgctcgg cttggagcgt actcgcatgt gcgagacgct ggacgacacg
     1681 ctggagctga cgccgggctt cacgacgtcc atcggcgagg gcgggctggt tgacgtccgc
     1741 ggacggtacc accctggatg ggtcgcgcac ggtgtcgtgc agtcgaccgt ggctgagctg
     1801 gccgttgtcg tcgacgccct gctggctgga cacctgcttg gacagcagtc gctggcggtc
     1861 atgcgcgccg cgctgccagt tctggtcacc catccggtca ttcgcgtgcc cggctacgga
     1921 ctcgggctca tgatggatct cgatccgggt tcacatagtc tgaccggaca tgcgggcggc
     1981 ggtccgggct actcggcagc ggcattctcg atcaggtctg gagacggacg ccggataacg
     2041 attgccacac tggcgaatcg ggacgcggat gaggtggccc tgaggatggt ggtcgagctc
     2101 ggtggaatgc ttgccggaac gtcctgatcg gctgccggaa tgcggtaagg tgcgtggtac
     2161 catgccagca gaatcgcaag cgagacctgc cggaaactgg cgtgatcgac ggaattggct
     2221 ccgccagccg caagaatcgg cacctgcgtc ggcagggccc ggagaggagc aaggcgagat
     2281 ggcaacggac gtcgcgcggc gtacgggtcg ctctaaggtc acggtggtcg gtgccggcat
     2341 ggtcggcggg actgtcgcgc agctggtcgc acagggcgac tacgccgacg tggtgctggt
     2401 cgacatcgtg gaggggatgc cgcagggcaa ggcgctcgac ctcatgcagg caggcccggt
     2461 gctcggctac gacagtaggc tgaccggcac gaacggctat cgggagacag gtaattctga
     2521 tgtggtcgtg attacttccg gcattgcccg caagccgggg atgagccgcg atgacttgct
     2581 gaagaccaac atgggcatcg tagagtccgt gacgaaggac atcgtcgcga cgtcgccgaa
     2641 tgcggtgatc atcgtggtgt cgaatccgct cgacgcgatg tgccacgttg cgttcgatac
     2701 ggccggcttt ccgcgcgaac gggtgatcgg ccaggccggc gtgctggatt cggcaaggtt
     2761 ccggaccttc atcgcaatgg agctcgacgt ttcggtcgag gacgtctccg ctttcgtcct
     2821 cggcggtcac ggcgatacga tggtgccgct ttcgcggtat tcgactgtcg ccggcattcc
     2881 gatcaccgag ctgatccctg cggataggct cagggagatc gagcagcgaa ctgccaacgg
     2941 tggcgccgag atcgtcagct tgctgaagag cggcagcgcc tactacgctc ccgccagctc
     3001 ggtagtggag atggttcagt cagtgctact cgacaagaag cgcatcctgc cgtccagcgt
     3061 cctgctgcag ggtgagtacg gcatcagcgg tctctacgtc ggtgtccctg cgaagctcgg
     3121 cgcgaacggc atggagcagg tgatcgagat ttcgctcacc gacgaggagc aggcagcgct
     3181 ccagaactct gccaacgcgg tgcaggggct tgtcgaagcc atggagcgcc tccggaacga
     3241 ggcttagagc cgcgttaact gtcggaaaac gtcgcagcct cggcttgttg ccgaggctgt
     3301 ccggctgtgg tggcactcgt cgtgcatcac ggctgatggt atggtcgcag gcagatgaat
     3361 ccgcctgaag atgcgctatg agagcgacag cttgctgctg ccgcctctgc ccgtaaagaa
     3421 gcgagaggat cgacgggatg ccgaagatac tgggcgcact cggcacggtg cgcgggttca
     3481 tgaacgtcgt gaaggaggtc gacttcgacg aggttcggag ccgggccgag acggcgcctc
     3541 gagtgctggt cgttgccgac gaacaggggc gagcggagcg gtttgtgacc gaggtgttcg
     3601 gcgacgacgc gatgcggcac gtcgacatcc gcacgggctg gttcgaggac atcgatgcca
     3661 gcagatggga tgtgattgtc gtgcttgacc cgaccgacag tggcgtcctg ccgaaggtga
     3721 aatcagctgt cggtgaagcg gcgaaacgca agctggtcga tgcgaaagtc tgcggaccgg
     3781 ggatgacggt ggaagagatc actgcagcgc aggacgcgat agtttcggcg gacaccgagt
     3841 ttgccgccgc attgggccgc cgtttcccag agttgcagct ggctgcagtc aaggcgatcg
     3901 tcgacgagac ggcgaaggcg aacgcacagt tcgccctggt ttccaacgcg ccggccatcg
     3961 tgccgttcat tggcgggatc gtcgcggcaa gtgcagactt gatcgttctc accaagaacc
     4021 aggtgatgat ggtctacaag atcgccgcgg tgcacaaccg cgcgctggac aatcagctca
     4081 agctcatgcg ggagcttgct cctgtcgtcg ggagtggctt tctctggcgc acgctcgcac
     4141 gggaggcggc ctccttcctt ccactggcag ccgggacggt gccaaaggtc gccattgcct
     4201 acgtcggtac cgtcgtgatg ggacgcgcgg cagactacta ctatcgcttc gggaagaaac
     4261 ccacgaggga acacctcagg gagtttaccc gaagagcgag cgagacggcc tcccggctgc
     4321 agatcagccg caaggacgag caggaggcca ggaaagcaga gcctgccgaa actgccgaga
     4381 ggcagcagcc gacagcggag gtttcctgag cgataaaggc acgcgctcca cccgtttgca
     4441 tgttatcgga ccattgcgcg ttggacgcta gccggggctc agagcagaga tccgcttgtt
     4501 gaactggggc accttgaggc cgagcagcgc tgcggcctcg gagcggtcgc ccttggcagc
     4561 ggcgagcgcc tcctcgagtg cggcatgctc cacctcgtcg aggaggtcgt cgagcgaggt
     4621 gccacgtcga accagggtgc caatgtcgaa gagtcgccgg ctgtcggcac cgaagaagct
     4681 gatgtgctct ggcgtgatga tcgatccctg tgagagcacc acggcgcgct cgacgaggtt
     4741 ctgcagttca cggacgttgc ccggccagtc gtagtccatc agcatctcca cggcctgctg
     4801 actcattcgg gacggctcgg accgaggcgt ggcgcggtac ttgctgagga agtgctcaac
     4861 cagcaacggg atgtcctcgc gacgctcccg taagggcggc aagtagatgg cgatcacgtt
     4921 gaggcggtag tagaggtcct cgcggaaccg gttggcctcg acctcctgct ggagcacctt
     4981 gttggtcgcc gcgatcacac gggtgtcaac cttgactgac accgtgccgc cgacgcgctc
     5041 gaactcgcgc tcttggagca cgcgcaggag cttcttctgg gtggaaaggg tcatctcgcc
     5101 gatctcgtca aggaagatgg accccttgtt ggcgagctcg aaccggccct tgcgctggtt
     5161 catcgcgccg gtgaaggcgc ctttttcgtg gccgaacagt tcactctcga gcagcgtctc
     5221 cgggagcgct gcacaattca ccttgacgag tggaccgcga gcgtagttgg acgtctcgtg
     5281 gaggatggtc gcgatgagct ccttcccggt ccccgtttcg cccgtgataa gtaccgtggc
     5341 gtcggagcgg gccacccggc ccaccgtttt gtagacctcc tgcatggccg gactgttgcc
     5401 gatcatcaga tcgttcggat ccggcttgtc gagccgcttg gagagttcct cgatctgatc
     5461 cgaaagctgc ttccggtcaa agaagcggcg aatcgtcagc atgacgtcgt cgaggtcgaa
     5521 cggcttggtg aggtagtcgt aggcaccacc ctgcatggcc tggatggcga cactactcgt
     5581 gccgaaggcg gtcatcatga tgatggggag gggaccgttg ttggttggct tcacctccct
     5641 gaggacgtcg atgccgctct tgtccggcat gcgcacgtcc atcatgatga ggtccggcgg
     5701 cgtgtcaccg gagagggaat cgatcacggc cctgcccgtt tcggcttcgg cgacctcgaa
     5761 cccctcgtcc tcaagaagat cacgaaggac ccctcgaatg gcagcgtcat cgtcgacgat
     5821 catgattgtg gacatcagtg gcggcgtatc cttgtctcgg catgagtggc gaccccggtt
     5881 ctgtgcgcgg ccgcggcgag agcattctgc accaagtata cgcacccact gattttcagg
     5941 ggagaacgag gactgcaggc acgatgacct tccgacgcgt ggtgttgatc ggcatgagcg
     6001 gggcgggcaa gagcaccgtc ggccgcatgc tagccgggcg actcggctgg cggctcatcg
     6061 atctcgacgc cgagatcgag cggcgccaag ggcgctccat tcccgatatc tttcgaacca
     6121 gcggtgagga cgtgttccgg gcgatcgagc ggcaagagtt tcgggcagcg ctggagagcg
     6181 agaacgcggt gatctcgaca ggcggcgggg cagtgacgaa cgaggaggcg tgggggagcg
     6241 atctgctcgg tgccagcggc acgctgtccg ttctgctcga cgcgccggcc gaggtgctct
     6301 atcaacgact ggtggagcag caggcgcagg acccgagcgg cgcgattcgc ccgatgctgt
     6361 cagggagcaa tccgcttgcc cgcatccggg cgctcctgag tgagcgggac cggtggtacc
     6421 ggcgggcggg catcgttctt ccagttgggg atcgtaccgt cgatgaggtg accggcctcg
     6481 ttgccgatgc cctcggccgg cgctcatggc tggtcgggct ggacgtttcc ggagcggcgt
     6541 cgcgcatcta cgtgggagcc gacgtgctcg acttcctggg agtcaggatc agcgagcagt
     6601 ggccaaaggc gcgccgcgtc tggatcgtcg ccgacgagga cgttgccggt tatcacctcc
     6661 aagcgacggt ctcggctgct gaacgagacg gcgcggaggt ccggacgctg acattcccga
     6721 gtggggaggc gagcaagtcg ctcgccgggc tcggcggact ctacgatggc ctgctcagag
     6781 gtggtatcga acgatcggat gtgatcgtcg cgcttggtgg cggtgtggcc ggagatctcg
     6841 tcgggttcgc ggccgccacg gtcttgcggg ggggcggcct ggtgcaggtg ccgaccacgc
     6901 tactggccat ggtcgacagc agcgtgggcg gcaagacggg catcaaccac ccggccggca
     6961 agaatctcat cggcgccttc taccagtcgc cggtggtgct catcgctccc gaaatgctga
     7021 agacgctccc aaaccgcgag tatcgctcgg gatgggcgga gattatcaag cacggactga
     7081 tcgagccgac gacgcctgct ggcgacagcg gactgtttga tcttgttgtc gcgaacgcaa
     7141 ggttgctgaa tgaccgggct tcgccgttgc tgaatccaat cattgcacgc aatgtcgaga
     7201 tcaaggcgtc tgtcgttcaa gcagacgagc gtgaggccgg gttgcgggcg attctcaact
     7261 tcgggcacac catcgggcat gccattgagg cggcaggcta cacgctgctc cacggcgaag
     7321 caattgcggt cgggctgcac ggtgcgatgc ggttcggcga ggcgctgggt cgggtttcca
     7381 gcgatcgtgt cgacgaggtc gtatcggtgc tcgaagcgtt tgggctgcca accactgtcg
     7441 atatcgatag cggcgagatc cggcggcgca tgcaaagcga caagaagcgg gtcgccggcc
     7501 gccagcagtg gatcgtgccg ctccccgagg gcggcgtgag cgtcgagcgt ggcgtgccgg
     7561 agacggcggt cgacgccgcc ttggcggcca tcattgcgcg cgattccgcc tgacgggacg
     7621 cccgtggtat cctcttgacg gtaaccggta ccgttcagca gcaccggcct cgtggtatgt
     7681 actgcctaat gcagacctcg cgaaagccga acggtgcggg cttcgtgcgt ccagaggaag
     7741 tgggggtccc gtcgcgtgac tcagttgcct aacatcgtgg tcagcgcatc gatcgcgttt
     7801 gaccacatca tgtcgttcaa cggctcattc aaggatcata ttctcgtcga gaaggctcat
     7861 gtgctctccg tcagctttct gatcgattcg ctgaaaaagc accggggcgg ggtcggcggc
     7921 aacgtcgcct acagtcttgg tcttctcgga gagcccgcgg cgctcgtggg cacggtcggc
     7981 gaagatttcg gcgagtatcg atcggtgctc gaccagcatg gtgtcgacac gagcggcgtg
     8041 gtagaggtcg gtggtgagct cacggccacg ggtttcctga gcgcggacct gcatggcaac
     8101 cagatcgtct cgttctaccc tggtgcaatg tggaagtcgc gcgagatcga tctcaccgac
     8161 gcggcgaacg gcgccgtcta tggcgttgtc ggtgccgggg accccgaggg catggtgctg
     8221 cacacgcggc agatcgctgc gtcgagtgcg aagctcgtgt tcgatccgtc gcagcagata
     8281 cccattctta cgggcgacca gctcaccgag ggcatcgagg cggccgacat catggtcgcg
     8341 aacgactacg agtactcaat gatcgaacgc aagactggtc tgtcgatcga ggacatccgg
     8401 cagcgtgtcg agctcacggt cgtgacctat ggcgagcagg gctcggagat acgccacggc
     8461 gacgagcagg tggagattcc aattgccccg gcccgcgagg tcgtcgatcc ttccggcggt
     8521 ggagatgcgt accgcgccgg gctgctcaag gggctgctcc tgggcttcga tctcgatatg
     8581 gtcggtcgag tggcagctca ggcggcaacc tatccgattg agaactacgg aacccaggaa
     8641 catgtgtacg atgtaccggg tttcatcgcc cggtttgagg agtcgttccc ggaatacgcc
     8701 gggcgtctgc acgagtccga catggttgcg gctatagccg atagaagcta gcatcgcggt
     8761 tcacacaccg caggaaagag agaagacgac accgatggcg accccgtcca cggatacgcg
     8821 taacttcgac atcaaggatc cgtccctggc accgggcggc cgtcgccgga ttgactgggc
     8881 agcccgcgag atgccggtac tggcccggat tcgcgagcgg ttcgcgaaag agcagccgct
     8941 caaggggctt cggctggtgg cttgcgccca tatcaccaca gagacggcca atctcgcgct
     9001 cacccttcag gctggtggcg ccgaagcggt cctctgcgcg agcaatcccc tctccactca
     9061 ggatgatgtt gccgcgacgc tggtcgagga ggggatcgcg gtctttgcca tcaagggcga
     9121 ggatgccgag acctatgatc gacacatcca ctcggcgatc gcccacaagc cgcacatcgt
     9181 gatcgacgat ggtgcggacg tcatcgccac catccatcga cactatgccg agatgctgcc
     9241 ggacatcaag ggcggcaccg aggagacgac gaccggcgtg atccgcgagc gcgcgatgga
     9301 gaaggccggg gtgctggggt tccctatcgt cgcagtcaac gatgccgaca ccaagcactt
     9361 cttcgataac cgctatggca cgggtcagag caccatcgat ggcattctcc gcgccaccaa
     9421 tatcctgctc gcgggcaaga ccgtcgtcgt cggtggctac ggctggtgcg ggaagggcat
     9481 cgccatgcgg atgcgcggac atggtgccca ggtcatcgtg accgagatcg atccgacccg
     9541 cgcgctggag gctgcgctcg atggattccg cgtgatgacg atggagcagg cggcgcctgt
     9601 cgcggacttc atcgtcacgg cgacgggcaa catcgatgtc gtcgaccgtc gtcactttga
     9661 ggtgatgaag gacggcgcgg tcctcgcgaa ctcgggccac ttcaattcgg agatcaactt
     9721 gaaggccctc ggcgaaatgg cgggcgaggg ccgccgacag ctccgcccgt tcgtcgagga
     9781 gtactccttc aacggcaagc gggtcatggt gctgggcgag ggccggctca tcaacctggc
     9841 tgcagcagag gggcacccgg cgagcgtcat ggacatgagc ttcgccaacc aggcgatggc
     9901 ggccgagtac atcgccaagg aaggggagca cctggaaaac aaggtctatt cggtgccgga
     9961 ggcgattgac atggagatcg ctgcgctcaa gctgaacgcc atgggtgtcg aaatcgacac
    10021 gctgacgccc gctcaggaga agtacctgaa ctcctgggag accgaatagc ctgtcggatc
    10081 agcagtcccg gcgaccggtt tcgcggtcgt cggcgagaag gaacacgcca tgtcgacgac
    10141 atttgatact gccgagcgcg tgctgttcac ctccgagtca gtgacggagg ggcacccgga
    10201 caagatttgc gaccagatct cggacgccgt gctggacgcc gcgctcatgg acgacccgag
    10261 cagccgagtc gcctgtgagg cgtcgacgac ccgcggcctg gtgttcgtat tcggcgagat
    10321 gaccaccgaa acctacatcg atatccagga ggtcgtgcgg caggtgctca ccgacatcgg
    10381 atacacacac tcgagccacg gcctcgacgc cgatacctgc ggcgtattcg tgtcgttgcg
    10441 cgagcagagt ccggagatct ccgcgggtgt caccactgcg ctcgagagcc gcagcggcag
    10501 ttccgcggac gaggccgatc tgaccggcgc cggtgatcag ggcatgatga tcggttatgc
    10561 gtgccgggag accccggagc tgatgccgct gcccatctca cttgcccaca agatcacgcg
    10621 ccgcctcaca gaaactcgca aatcgggcca gctggcctat ctgctaccgg acggcaagag
    10681 ccaggtgacc gtggagtact cgcacggcag gccggtcagg gtcgataccg tagtggtatc
    10741 gactcagcac aagccggacg tgcccaatgc ccagatcgag cgtgacgtcg tcgagcgcgt
    10801 ggtgaaagcg gttgtgccgg ccgacctgct ggataccaac actcggtacc tggtcaaccc
    10861 gagcggtcgc ttcgagatcg gcggcccggc ggcagacgcc ggcctgacgg gccgcaagat
    10921 catcgttgac acatacgggg gcatggcacg ccacggcggc ggcgcgttct cgggcaagga
    10981 cgccaccaag gtcgaccgat ctgcagcata tgccgcccgc tacgtcgcca agaacatcgt
    11041 ggcggctggt ctcgcggacc gcttcgagct tcagatctcc tatgcgatcg gcaaggcgca
    11101 gccagtctcg atcatgtgcg agaccttcgg taccggccga atcgcggacg accagatcga
    11161 gacgcttgtt cgcgagcatt tcgacctgcg gcccggggcc atcatccgca gcctcgatct
    11221 gcgccgtccg atctaccgcc agacagccgc ctacgggcac tttgggcgtc ccgacctcga
    11281 cctgccttgg gagcggaccg acaaggccga tgcgcttgcc agcgcagccg gctttgcgca
    11341 ggccgccacc gctgattagc ctgcagccag aaggcccctc tcgcggccag caccttctcg
    11401 catccgacgc gcgccactcg ctgggacctc gctcgcatga agttcatcat cgcggttatt
    11461 caggcgtacg atactgaccg cctgctgcga gccgtcacct cggcaggtct ccgcgggaca
    11521 aagatcgtga gtactggcgg gtttctgcgc atggccaacg cgacgatctt tatgggcgtc
    11581 gacgacgcgc gggttgaaga atgccttcag attattcggg agcactgcga gtcgcgaatt
    11641 gagtcgtcga tcgatccagg cgagcaggag tacatcgagt ggttcccgat tggcgtgcac
    11701 gaagtcaccg ttggcggggc tgtggtcttc atcgtcgggg tcaacagctt ccaccagatg
    11761 ttcccgccca gcccagcgag cgagcggcaa cgttgactgg ggggctcggg tggcggtcca
    11821 tcgcgacgac gtggggcggt gccgctctgc tgctgatgcg gcaattcggc gccccctcag
    11881 cttcgggtgt gaatgcagag acgcgcgtgg tccgggtggc cgatgccgag gcagaaatca
    11941 tcgcctccga gcgaacgctg gacggacgaa tcctgacgct accggtcgag ttgggggcga
    12001 aacgggagcg tcagaatgct aggcacctgt cggcgagtcg gttcaccgtc aactagctaa
    12061 cgacggcctg caggctttcg atctcctccg agtcgtagcg cgctcggtca ggaagcatcc
    12121 ttcccaggat gtgctgaatc gccatgatat gactgcatgt tccaagcatg tgcgtctcga
    12181 aggtatggca ggtgcattcc caccccttct cggtcaggga cacggcgtag ttgtcatggc
    12241 taccccggaa ggaggcggtg aacgtctcga aggtgatgcg ctccggttcg cgcgcgtacc
    12301 ggtgggcctt ctcgatcttg ccgatcatgc ttgagttcat gagccatcgc tccttctgct
    12361 ttccaaaccc cctcggttcg gacgacgctg ttggttacac caggaccgtg acctcagcac
    12421 gtgctcgacg ccgatgcggg gactcggctc aaacaaaacg cacccggcag cgaatgtcgc
    12481 gcgggtgcgg gtcatgcata tggatgtttt cctgcttgaa cggatgccat gggtacctcc
    12541 tgtttccccc acgtgccgat ccggttcacg gtcttgaatt acaagggatt gtaccagccg
    12601 gcatgcccgt ccgccagcga agatcatctt cccagtggtc gcatccggag cgcgccagcc
    12661 gatacggaac tatcgagcct cggcgctata ctagcgccag tgacgatcga tattcgacct
    12721 tggagagacg tggatgtccg cgaacatgga ctcgctcagc attctgcagc gcgcgcagtt
    12781 ggcgtggaac ctgatgcggg atcaacgcgt ggcgggctgg gtgaaaaaag ctggccctgc
    12841 cgtgattctt gtctacgtca tctcgccgat cgacgtcatt ccggatttcc tgctcggcgc
    12901 gggtcaggtg gatgatgttg gcgtaatcgc gctcggcctg ttgctccttg tacgactgct
    12961 ggtgcggttc gcgcctcagg aggtcgtcga cgagcacgtc gcccgcatca ctggcgttcc
    13021 gggcgccaac cggcaatcgc gacggagcag gacggacggg ccgacgattg acacgagtgg
    13081 tcgggtccgc taggacgaca ggacacccag ggaaggacca tgccaccagc ccatcgcaac
    13141 acatcgaagt cggccgagcg tcggcggcag cgacggcagc gtcgaagcgg ggaggagcgt
    13201 ccacggcgga gcagccaggc cccgaaggtg gctccgctgc agctcgatcc acgcaagacg
    13261 gcctcatcct ctgatgcaac ggcttcagtg ccgacaactc aactcgagcc ggaaaccgag
    13321 tacggaatcg tgcggcgcga tctccggcgc ctgacaatct tctcggttat ctgctttgcg
    13381 ctgatgattg ccgtcctgtt cttcctcaac gtttgacgta ggagccatgt agcgccatgg
    13441 tgttgcaact gatcacaaga tagccgggtc gcatgagcga accggctgtc ttgtctccga
    13501 tgcctggtat gcgccgggtt acatggcgtc gtcatccgtg tcgccggcga gggaactgtc
    13561 gacgtactgc gctgcctcct cgtcgacttc cacctcatcc tcctcgtccc cgacttcctc
    13621 gtcaaccagt tcctcctcat cctcctcgcc gaacgagaag tcgccctcct cgcgcaagga
    13681 aagaagctcg cgctcgcgca tgaggtcgat ggtctgccgg ctctggcgcg gtaggccggg
    13741 ctgaccctcc attgagggat cctgatagga ctcccggatg atgatgcgga ggctgtggcc
    13801 cgacatgccg agggcgtaaa tttcgtaccg gttgccggcg gcgatcaact cctcaagtcg
    13861 ccgggagatc cgctgctcca gctcgccgag ccgttcgccc gtgcgggagg aaacgacgat
    13921 gcggctattc tcgacctgaa gggtcaactg ctcgccaggg gagacagtgg caaggacggc
    13981 ctcctcggtg gagttgatca attcatcgac ccacgtcttg ccgacctctt cgatgaagac
    14041 gttggcgcgt ggcgaaccga tctgctcgtc cgacactcgg tccgaaacgt cggtattgac
    14101 gagcatctca aggcgctgca ggttacgacg agcgatcatg ttggccgggt cggccgcgag
    14161 tgcttcggag taggcatccc acgcatcctg cagccgtcca agctccagca acgcgcggcc
    14221 tttccggttg agcgcctcgg cgtcgttcgg aaagcgctgg aggaaggagg cattgatctc
    14281 cagcgcctgc tcccaatcac cggcgagggc gatatcgcgg gcctgctcga ccatctggcg
    14341 cttgttgggc tgctgctggg ggacttcctg agtatcggtc aatgtgacgg tcctttcgac
    14401 agtttagctg ctaagaaggc ggagtggccg gtcagcgcgg cgcaccaacc ggctcgccca
    14461 tcaggaacca cggcgatgtg cgggtgatct gcacgtcgac gaggttgccc gtgagctcgc
    14521 gatcatcatc gaagtgcaca agggtattgg ctcgggttcg accagtccac cggccgcggc
    14581 tcttgccttc gacgagcacc tcgtgggtct cgccgaggcg cctgctattg atgtcggtcg
    14641 cgatgcgctc ctgctgcttc tcgagcatct ggtgacgacg cagcttctcc tcgtgcggga
    14701 tgtcgtcgtc ccaacgtgcc gagagcgtgc ccgggcgcgg agagtacatt gcgaggtgga
    14761 ccttgtcgac tttcgtctgc tccagcatgt cgaacgtctg catgaactgc gcttcggtct
    14821 cagccggaaa gccgacgatg acgtcggtcg ccacggtgac acccggcatg cgctcgcggg
    14881 tgtaagcgat gcgctccatc cagaggtcac gcgtgtaggt gcggcgcatg cgcctcaaca
    14941 cctcgttgtc gccggcctgc accggcaggt tcatgtgctc gcaaatcgtg ccaagctccg
    15001 ccatggtgtc gatgagctcg tcagtcatgt acttcggatg cgaggtgagg tagcgaaggc
    15061 gctcgagccc gggcaccttg tcgaccgcgc ggagcagctg cgcgaggtct tgcggctccg
    15121 ggaagtcgtg accgtaggcg ttgacggtct gcccgagcag cgtcacctca cgcacgcccg
    15181 tctcgaccac gcgctccacc tcgcgaacga tttccttcag cgggcggctg gtctccttgc
    15241 cgcgacggta cggaacgatg cagtaggagc agagaaaatt gcagccgtag atgatcggga
    15301 tataggcggt gacagccgac gtaccggtgt gctgctgcgg caggtagtag tgcggcagct
    15361 gctgcaagtc atcctccagc tcggaaagct ccgggaccac ctgctcgagc ttgtcgaaat
    15421 cagatggatc gaagaaaagg tcgacgtgcg ggaacgcctc acgcagcttt tcctgctgct
    15481 tggtcaccat gcagccggtc aggacgaccg gtacctccgg acggttacgc ttgagccgca
    15541 cgaggctgtt gagcttgcca gataccttgt cttcggcggc ctggcgcacg acacaggagt
    15601 tcagcacaac gatgtcggcg tcgtcctcag agcgcgttgg ctcgtagccc acctcctgca
    15661 acatggccgc gaccttggcc gaatcggcct cgttcatctg gcagccaatc gtccagatgt
    15721 agaaccgctt gccgttgcga gggagccgtg ccgggctcgt cttcggctga atttggataa
    15781 gcggactcgt tgacacagga atctccaaac gggtgcaaac actcaaagac gctctgcgaa
    15841 aactcgcaga gctatccggt gcctcggccc atgattatag cggatgcttc cggtggttgg
    15901 cgtaccgggt ttcgctgccg acattgccat gtacgtgcac taatggttgg tcgactgcag
    15961 tggtagtctt gtcgcagatt cgagcatccg ctcaggtgag tcggcgtggt tgtaggatgc
    16021 cagttgctcg tagggagcct ccgccgtgtt cgtccgcacc ctctccagaa gttgggggcc
    16081 actgcttgtt gcatcgctcg ccgtcgttgc gatgctgaac acatcgatgg ccgccgcacc
    16141 gttcagcggt gctcctcagg cacgtctggc ccaagtcaac gtcgaatgga tgtccgagga
    16201 gcaggcagcg gcgctgaatc tgagcttccc ggtcctcgtg ccgagctggg ttcccgcgcc
    16261 attcgcaggc gcaccctccg tacaggccgg gggggggtac tacagcctct actggatggt
    16321 tcccggaggt gatccgacct tcctgcaggt cgccggcgag gtcggcggcg ctatgcccgt
    16381 cggatcccca gccgatctga acgtgcgact tgagatcaac gcctcggtca acggctatga
    16441 cgcaatccac gacgtgacgg cgatctacga taacgtctgg tgggtccagg gcggcgtact
    16501 ctacacagtc agcagcaaga acatgacggg cacagacagt ctcgccctag cgaactccct
    16561 tgtgcagctg cagccgcctg tgcagccgcc tacttcaccg ccaccaaccc caacgccgga
    16621 gccggtggag tcgtcgccat cgggcgcgat ctacaacgcc gcggaggtcc cgtcggcgaa
    16681 catcgcgacg atcgaggcgt ggccgagtgc gacgagtacg ctgcgggcga cgcacggtag
    16741 cttctcggcc tccggagcgt cgttcctctc ggtggacggt ggaattttct catggcaggc
    16801 gccggtggtg gatcgcaata ccacggtcga gttcacgctg atcgatgacg cgaccggaga
    16861 ggtgaccgca tggtcatcca ttgtggtgta tccgacgtca accggcagct ccacattgcc
    16921 ggtgcaggag ccaatcacgg agccggaaac tggagtgtcg tccggcccaa cgtccatgcc
    16981 agcagacgat gcattgccga gctcgatcac gagcacaagc acagcggcgg tgagtgagag
    17041 cgcatcccaa ccaacaacta ccatcaccac aagcgcgact gacgaggacg agggagacac
    17101 gtcggcgagt tcgagcaccg gggatgccac aacgtctgtc gaggcggaca cgggcacgag
    17161 cgagaccgtc acccgggacg agacaccgga ggctggcgga gccggtggcg gcttgacgtc
    17221 cgatggaact gccgggccgc cgatgccgcg taccggtgat ggaacgtctg gccccagcct
    17281 ccctgcaggt ggtgacggga ccggcggcat ccggcagatc gcggtcccat agatcgcggt
    17341 caaacgatgg atgccagcgt ccggctgcct gcgtgtgacc gtcaggcggc cgttttacgt
    17401 gcgctcatcc gctggttgtt gggctcaagc agcacgacga atcgtgtgcc ggcggtgggc
    17461 gcgtcctcga tcgtgattcg cccgtcgtgt gcgcggacca actcggagca gatgtagagg
    17521 ccgagcccga gacccggcat cgcaccctcg gatgcttcgc gtcggcggta gaacgccgaa
    17581 aacagctgtt cgcgctctgc ttccgggatg cctttgccct cgtcggtcac cgtgatttcg
    17641 agaccccagc cctcgcgggt cgatggaccg actccgacag tgatcgcgcc accctcggtg
    17701 tacttgatcg cattaccgac gagattgtcg ataatttggt gaacacggtc ctcatcgaca
    17761 ttcgattcgt ggcctgacca aggcgtctcc agtcgcaccg tgtgggcagg ggtggaggcg
    17821 ttggccgagt ccaccacccg tcgcgcgatg tcgaccacgt cagctggaga ccgccggatg
    17881 gtcagacggc cggcacgagc gcgggagaca tcgagcaggt catcgacgag gcgagtcatg
    17941 cggtcggcat ggcgctgaag cgtttcgagg taggcgatgt gctgcgcgtg accgccctcc
    18001 gccgcaaggt cacgcgccac cacctgcgcg aaaccccgga tcggtgtcaa cggtgatcgg
    18061 agctcgtgtg agacgacgga gacgaactcg tcgcgcatcc gctcggcttc cttcagtggc
    18121 cggacatcct gaaagacgcc gacgacggcg tcgaccgtgc cgtcgtcggc ccagagcggc
    18181 gccgcgctgc caatcaccgg gagcgggtcg ccacctgcct ggtgaacggc caactctacg
    18241 cccgtgaccg tctcgccaga cgtcagcgct cggttgatcg gcaagtcctc gacggcatag
    18301 tcgccatcca agcgcagctg atcgacgcgc agcgaaaagg ggttgccggt ggcgggcagc
    18361 cggaacattc gcgtggcggc ggcgttttgg gcaacaatga tgccctgcgg tggacgcacc
    18421 accatgactg cctctggcat ggtttggatc accgcctgga ggcgccgatg ggagtcggag
    18481 atgcggccga gaagttcttc ctgagcatct gcgatgctcg cagactccat aaacgcaacc
    18541 gcgcggtggg caagcgcctc aaggcgggcg gttgccgttt gtgcgaaccg gtaaggggca
    18601 tagaggccta cccaggcaac gccgacgacg cgctcttcgg caccgaagag cggcacgccg
    18661 aggaccgacc gtacgtgccg gttcacctcc agaacgtcac cgatctcttc cggcattaca
    18721 gtgctcgtgt cgttgatcgc cagggggcga cgctcccggg cgacgcgccc cacaaacccg
    18781 tctccaaacg ggatctcaac actagggcgc atctggtgcg cggtttcctc ctggctacca
    18841 taggtgactg cctgcacgag ggcattggag ggaccgtcgg tcaggtagat cgcgccagcc
    18901 gacgcccggt tgacgcgcac cattgcagtg aggatgttct cgaacacgga gatgtaggct
    18961 gcgttgtcac cggcagggcg ctgggcggcg gcgatctcga ccgcgttgag cgcggtgttg
    19021 gccgcacgca tagcggcggc atcaagccgg gcgctggcct ggtcgtactt gaggcgctcg
    19081 acggtcgcgg cgaccgcccc agcgaggatc aggaacagca gcaaaacgac gatgccgcgc
    19141 ggctcattcc acgcgtcgag cagccagcgc tcgccgagca gtgtcacatc cagcagcacg
    19201 gcgcctgcga tggtggctgt cacgccgccg acccagtcag ccacgtaggt cgcgagcata
    19261 accgacacaa caagtagtgg cgcgacgagc gcggcgtcga cctccggagc cgtgaggctg
    19321 atcagcagga gtgggacggc gatggtgccc actgcgaaca cgagtcgcca gagttcgacg
    19381 cgcgcctggc gcatcgaggt gacctgatca ctgccgatcg acgcccaacg gcggagggac
    19441 tcggacgact ggtgcattct ctcggtggtc gagtgcgccg ctctggtgat gcgatcccag
    19501 gtttcgggag ccatccttgc gcgcgcccac cccgcgaccc gcgattctcg tggctcttcg
    19561 gtcggttcca gttcgtcctg gcgcatacag atcggtcccg tgataaaggt ctattgcgat
    19621 gcttggcgct gtccggtctc agccatctcg ccgtcgatga tctcgccgag aatgcggatg
    19681 ccctcgccga tctcctcgtc ccgcgcgaac gagtaggaga ggcgcagcat gttctggcct
    19741 gagccgtcgg tgaagcagga gcggcccgga aggtactcga cgccgcgctc acgagcctgt
    19801 gcggccatcc gtgtggtgtc gacgcccttc ggtagggtga cccaaatgaa aaagccgcct
    19861 tctggctcgg tccagctgca tccctccgga aaatgcttct gaagctcggc gagcatgagg
    19921 tcgcgacgat gctggtagat ggccttgagc tcctcgatgt gcttgtcgag atggtcgggg
    19981 atccactcgg cagcgacgta ggagccgaag acattcgtgc tgccgtccac ctttagcaca
    20041 cccatcttgc tgacgagtgc cgggtgtgcg acaacccagc cgagccgcat gcccgcgccg
    20101 agcgtctttg aaagcgtgcc gaagtagagt gtcgtccccc ggttgtcgag gctgaagatt
    20161 ggtgggatct gctccccgga gtaccgcagg tcggcatacg cgtcatcctc gaggatgaag
    20221 gtgccgtact cgtgcgccag ttcgacgatg cgttcgcggc gctcagggga cgtcgagacg
    20281 cctgtcgggt tctggaagtt cgggatcgtg tagatgaact tagctgtaat gccgtttgct
    20341 ttcaggtcgg cgagcgtctt ctcaagagcc tccacgttgg taccagcagc gtcgaccggc
    20401 gcattgaccg gttgcgcccc ggcgttccgg aacattcgga ctgcgcccat ccaggtcgga
    20461 gcctcagtga tgatcgtgtc gccccagtca accagggctt cgatggccaa gccgattgcc
    20521 tggctcgcac cggcggtaat gagaatctgg tcctgctccg cctcgatgcc gaagtcgcgg
    20581 cgcagcttgt cgagcaggat ctgcgcgaga ttccgattgc cgagtccatg gccgtactgg
    20641 agtgcccact cgccctcggc ctcgagggcg gtcttcgtgg cctcgatcac acttgtcttg
    20701 ggcagcgagt cgcgatcggg gaagccggcg tagaagctga tgatctcggc ggacttgcgg
    20761 gggtcgccca ctcgcagacc aggcaggtcc ttcgcccggc ggctgatcaa cgactccaga
    20821 gggacggcct gcgtggttgc catgaagtga ctctcctggt gctctctttg tcgtcgccga
    20881 aacttcgctt tcagcattgt acggtcactg agggggcttg gccaaacgat tcatgcgccg
    20941 ggccccaacc cgcgcggacc atcggccggg ttcagatgca cgggcgtagt agaatcggct
    21001 tgacgaaagc tgctggcgtg agtgagcggc tgggcagcgc cgccttcggt ccatggccga
    21061 cgatattgtg caggagagac gattttggta agcaacgtga cgcctgccga cacccgcctc
    21121 gaggccaagg gcgacgagga tcgcatgttc gatatcaccg tgatcggtgc tggcccgacc
    21181 gggttgtttg cggcgtttta cgccgggctt cggggcgcgt cggtgcagat catcgactcg
    21241 ctggaggagc cgggcggcgc cttgaccgcc atctaccctg aaaaatacat ctacgatgtt
    21301 atcggtttcc caaaggtgct cgccaaggac ttcgtggggg cgtgtcttga gcaggccatg
    21361 cgctccggtc cgaccattcg cctcaatgag caggtgcagg atttgctccc gcaggaggat
    21421 ggcacgttaa tccttcgcac gagcaaggcg gagcgtcgca gccgctcagt gatcgtcgcc
    21481 gctggtgtgg gcgcattcga accgacccgg ctcacggcac ccggcgtacg agagctcgag
    21541 ggcaagggag tccactactt cgcaaagcgc gtggacgact tccgcgacaa gaatgtggtg
    21601 attgttggtg gtggggactc cgccgtcgac tgggccgtga cgctcgagcc gatcgccaag
    21661 accatcaagc tgatccaccg atccaaattc cgggcgcacg aggcgacggt cgaggagctg
    21721 gagcgatcga gtgtcgagct cttctacccg aaatgcgagg tcactgaggt ctggccgggg
    21781 cccgacgggc gaatcggctc gctgacgttc aagaacggtg atgacgagct aattgacctc
    21841 gaggtcgacg agctgatctg cgccatcgga ttcaaggccg atccgggccc gatgcgcagc
    21901 tggggattcg agttggcgcg aaaccagatc gttgtcgaca agatcaccat ggagacgaat
    21961 atcccgggcg tgttcggtgc aggcgacatt gtcagctacc ccgccaagtt caagctcatt
    22021 gcgaatggcg cggcggaggc ggtcacggcg atcaaccacg cagtcaccta tttcaacccc
    22081 gacgcgaggc tcgatccggg tcactcaacg acgatcatgg agaagcgggc aaaggaaacg
    22141 gccgccgatt aggtcgcgga cagctggtaa gcgccgttgt catagccccc tacagcataa
    22201 tcacggctga ggaagagaag acccgcccgg aggctgggcg ggtcttgctg tcgatgttgc
    22261 tgcggctacg gccggggtcg gccaataccc atacgctccc ggacgagccc catcttgccg
    22321 gaagccatgt cgttcgcctg ctcggcgcca cgttgcagaa cgccggcaac gaagccagca
    22381 tcatcgttga gtcgggtgag ttctgcctgg ataggtgcaa ggcggtcgac cacaacatcg
    22441 gcgagctctt tcttgaaatc gccgtagcgc ttgccctcga aatgtgcttc gacctgcgac
    22501 ggcgtcatgc cggtgagcga gccgtaaatc tggatgagat tggtcagggc cggcttgtct
    22561 ggtccggcgg tgacggtctc gccggaatcg gtgactgcac ggcggatttt cttccgaatc
    22621 acatcaggtg ggtcagagaa ggcgacgtag ctgttctcgc gcggcgagct cttgctcatc
    22681 ttctcgtccg gcttgtcgag cgccatgatg cgagcggctt cctccttgat gtccgccttg
    22741 ggcactacga aggtctctcc atagcgcgca ttgaaccgct ggacgatgtc ccgcgtgagc
    22801 tcgacgtgct ggcgctgatc gtcgccgacc ggcacctctt ccgcatcgta gaggacgatg
    22861 tccgccgcca tgagcaccgg gtagctgaag accactgcgg agacggaatc gcccttgccg
    22921 gctgacttgt ccttgaactg cgtcatgcga cgcagttcgc cgaactgggt gaccgagttg
    22981 agcagccact gcagttcggc gtgcgccggg acatcggact gcacgaagag caggcacttc
    23041 tgcgggtcca gccccgatgc caggagcgat gaggcgacgt tgcggatctg ctgctggagt
    23101 acgtcagggt cgggattgtt cgagagtgca tggagatcga cgacgcagaa gatgctgtcg
    23161 ctgttctcga tttgattgac ccagttccgt atcgcaccga ggtagttgcc gaggtgaaaa
    23221 ttcccggagg gctggatgcc ggaaaagacg cgaaccttct tgagctcctc ggcggtgtct
    23281 gcctggttgg atgcgcgatc gggagcgtgg gtcaccatga tgctgaatcc tcgattgctc
    23341 ttgcctgcga cgatgcctcc ggcggcgagg ccgccagaag cttaccaaac gcattccggg
    23401 catatgagac agatgttccc cgacgcctag cgggctgagg cggtttcggg agtgcggacg
    23461 gcttcacccg agcactcctc tggcagccat gccgctccga actcgcgcat catgtcgatg
    23521 acggggagga gggccttacc cttctcggtc agcgcgtact cgacgcgcgg cggcacctgg
    23581 gcgtagcacc ggcgggtgac gacttgggcg tcctccaagc gcttgagtcg ctcagagagg
    23641 gtcttggggc tgatgccgct gagcgaccgc tcgagttgac tgaaccgcct ctggccgacc
    23701 gcaaggtcgc gcaagatgag cggagtccac ctgttcccga tcaggtcggc tgtgcgagcg
    23761 accgggcagc tcaccagccg ggcctggttg cttaccatga aatatcctgt ccgcgattta
    23821 gccaagaacc gcgcctacga cataaagctt ccctgaggat agcaggtatg ccggtgcgca
    23881 tcaatcacgc tcgtggtcag atgcacggcg ggatttatac tggtcccggg acgctgcgcg
    23941 gcatcgcgtt tcatgctggg cggccggtgg gatgtgaggg agtacgaagt tcgatggtcc
    24001 ggtttatcgc gtatgtcgtc gccaccgtgg tcgccacgct cgcgcttgga acgatctccg
    24061 aacgactcat cgagttcgac cgagcggagt ccgttttgct ctttgcggtc gttatcggag
    24121 cgattaacgc cttcatcaag ccgatcgtga acttactgac gctaccgatc acgtgtctca
    24181 cctttggact cttcacgatc gtcgtgaaca tgctgctctt ctggatcggt gcccgcataa
    24241 ctcccaacgt ggaaattccg acatggtggg gggcgctctt tgggtcgctg ctggtcagcg
    24301 ttgcggccgg catcattttt acggtgatcg atgagtaggt acccgatcga ggacgagctg
    24361 gtcaccgagc gtcgccactt cggtgatcct ctcaccatcg gtgtcatgtc agacacccac
    24421 atttactctc gtggccgtcg tgtcgtcccg cccggtgtga tccgactctt ccgccgagca
    24481 cgaatcgacc tgctcgtcca cctcggtgac gtcaacagtc ggcacgtgct cgaggagttg
    24541 ggcgagttgg ctccgcttat cggcgtgccg ggcaacaacg atgagccgga gctgcatttc
    24601 atgcttccgg aaacgacgca gtttgccata ggcgagcgga cctttggtgt cctccacgga
    24661 catggtggat gcacggcccg cgacgaggcg atccggcgct tcgtcggcaa ggtggattgc
    24721 gtgctcttcg gccacagtca caagccgctg atcgaccaga tcgacgagac catcctcttc
    24781 aaccccggat cgccgacgga ccggcgctgg aacgagcact ttggtgtcgg gatcatccgc
    24841 gttgaggcag gtacgatcag gccggaactg atgcttttta ccgacgccga tcatctggac
    24901 aacatcgacg tcgccgacat cgccggcgag ggacgcttgg gggacgacga gtgaccaatc
    24961 acatacagga tccacggctg actgacggtc gttggcttcg catccccggc ccgaccccgg
    25021 tgccgccgcc ggtactagcg gcgatggcgg agcccatgat tccccatcgg ggcaaggaga
    25081 tgtcggcact gatccgtcga ctccgagaac gcctgcagga ggtccaccgc accgagggcg
    25141 acgtgatggt gtggtcgggc agcggctcgg ccggctggga ggcggcgctc cagaatagct
    25201 gccggccagg cgagcgcgtg gtggcgaccg tgactggtga cttcggccgc cgcttcgctg
    25261 acgttgccga gcggctcggg ctctgggtga cgcgggtgga ggcgccgtgg ggacaggcgg
    25321 tgagccctca gcaggttgag gatgcgatca agcgggcggg cggggtacgc gccgccctga
    25381 ttacgcacaa cgagacctcg accggtgtca ccaaccctct cagggagctc gccaaggtgg
    25441 cacggaggca cggcgcactg gtacttgtcg atgcggtgag ttcggcggcg gcgatgccag
    25501 tggaagtcgg cgcctgggat ttggactggg tgatctccgg ttcgcaaaag gcctggatgt
    25561 gcccgcccgg tttgatgatc gcggcgctga gcgagcgcgc cctccaggcg gctgccgacc
    25621 agagcaaccg gcggttcttc tgggacgtgg agaagatggc gagcgcgttc caaaacgggc
    25681 acgctacgac gacgcccgcg attccgctgt accgtgcctt gaatgtcgcg ctcgacatga
    25741 tgcttgatga gggagtcgag gcaatgtggg agcggcaggc ggcgcttgga gcatggctcc
    25801 gggaccggct gaaggggctc ggcctagtgc tctacgccga cgaggcggtc gcatccgcat
    25861 cggtgaccgc tgtccggcct ccggcaggta tgcccgcctc gacattgcgt gacatgatcc
    25921 tcgccgacag cggcatcgag gtcgccgtgg ggcaggggcc ggaaaccgag gcggtgatcc
    25981 gagttgggca catggggtgg agccatcggc cggaaatgga agccacggtg gagtcgatcg
    26041 cccgggtatt gagtcacagg tgacgcaccg gcgcgttgtc aagtccccag agcttcggta
    26101 tacttgacac atacctcctc ccacgagacc agcgctctcg agtggctgat cgtggtgagg
    26161 tttcgcatgt gtattcggtc ttaaggaagg tctatggacg gtactgacgt tcgtgagcag
    26221 ggcggagtga acggcgacat gcaggagacg caggtcgact cgcagagtgc agccggaact
    26281 gattacgagc aggagctcga tggcgcctcg ctcatggagc aattcctgag cgatcccgat
    26341 cacgactaca agaccctgaa atacggggat gtgctcgaag ggatcgtgat gcacaaggat
    26401 cgggacgaac tgctagtcga cattgggtcg aaggcagagg gcatcgttcc ggccaaggaa
    26461 tattccagcc tcactgacga ggagaaggag aaccttgcgg tcggcgacac cgttttggtg
    26521 ttcgtggtgc agcccgagaa ccaggagggg catcccgtcg tcagtatcga ccgtgcccgg
    26581 caggaaaaga gctggcgcca gctgcaggag cggcacgagg ccaacgacgt catcgaggcc
    26641 gaggtcacca actacaacaa gggtggcctg ctcgtgaatc tagacggcgt gcgcggcttc
    26701 gttccggcgt cgcaggtcac cgagatccgt ggtggagacg aggccagcaa gcaggccgac
    26761 atggcccggc tgatcggcac cagccttgcg ctcaaggtca tcgaaatcaa ccgtcatcgc
    26821 aaccgcctga tcctgtccga gcggcaggcc gtgcaggagc ggcgcgatgc gatgaaggag
    26881 cagctcatca aggagctgac cgagggcgag gtccgcaccg gcaccgtttc ctctctttgc
    26941 gacttcggcg cattcgtcga catcggcggc gccgacgggc tggtgcacct ctccgagctc
    27001 tcctggagcc gtgtccgcca cccgagcgag gtgctcaaca tcggcgagga ggtgcaggtc
    27061 tacgtcctcg gcatcaacgc cgaggagaag aagatcgcac tctccatcaa gcggacgcag
    27121 ccggagccct ggagccgcgt ggcgacgagc tacgaggtcg gccagctggt gaagggcacg
    27181 gtgacgcagc tcgcgaactt cggggcgttc gcccgcatcg aggacggtat cgaggggctg
    27241 atccacgtct ccgaactcgc cgaggagcgc atccagcatc cgaagcaggt ggtcgaggag
    27301 ggcgaggagc tcatcctccg catcatccgg atcgacccgc agcgccgccg catgggattg
    27361 agcctgcgtc gcgcgctcga gacgccggac agcgagctgg ctagcgtctt cggcgacgac
    27421 atcctcgccg agcgcgacaa ccttgcgcgc aaggtgcgct cgcggctcga ggccgagggg
    27481 ctggtcggtc aggcaccgca gccgacgccc gagcaagccg ccgctgcttc gcccgaggcg
    27541 ctggaggacc ttgacgagga ggccgccaag cgggccgccg gtgaggcaca ggtcgccgca
    27601 ttcgaggagc gccgccgccg cgccgaggaa gccgagcagg ctcgccagcg ccggcaggag
    27661 gagcgccgcc agcagcgtgg cgcgtcgtcc ggtgagtttg ccggcggtga catgtcggcg
    27721 atggcgatgg cgttcgccca agccggggcg agcgacctga acgacctcat ctccggcgag
    27781 gagcaggagg ccggcgagtc caagagtggc cagagcccga gcgccgccgc cgactcggcg
    27841 ggtgagaacg cccagccggc tcaggtagcc ggtgccgcgg gcgaggcccg cgaagatacg
    27901 aatgctgagg gtgatgccgc gctcgcagac gagaccacgg cccagcggct cgccggcgac
    27961 gagcagatcg agaaccctgt cagtgaggac gagaagatca ccgatgccgg ttccgaatgg
    28021 gataagacga tcgtcgagaa gggctcggcc gacgagggcg atgtggtccc caacccggag
    28081 acggaggaga ccgagaaccc gcagccgggc gagcagccca agggacagga gtaacactcc
    28141 tacccatacg ggtagggtga tcgtcaccca ccactgtgat acaaccatga gcaacggcag
    28201 gtgtagtcgt acacctgccg ttgttggcca tttcaaccgg tagtcacgga gatgacgtcc
    28261 gggtcggcgg ccggacgcgg tgggtctggc cgggacgatg ctggtgccgg ccatggcagg
    28321 cgcaaaatac gggagagctc tggatggcgg agaagtttga caagtttacg gaccgtgccc
    28381 gcaaagtcct cacgctcgcc caggaagaag cgcaacggtt caaccacaat tacatcggca
    28441 cggagcatct gctgctcggc ctcgtccgcg agggcgatgg tgtggcggcc aaggtgctca
    28501 acaacatggg cgtgcaactc cccaaggttc gctcggcggt cgagttcatc atcggacgcg
    28561 gtgagggaat gatcatgggg gaaatcggcc tgaccccccg cgcaaagaag gtgatcgagc
    28621 tctccgtcga cgaggctcga cgcctgaacc accactacat cggcacggag cacctgctgc
    28681 tcggcctcgt ccgcgagggc gagggcattg cagcgggcgt gcttgagagc ctcggcgtca
    28741 acctcgagaa ggtgcgctcc gaggtgatgc aggttgtcag ccagagcact tctggttccg
    28801 ggcagcagca aggctcgaag cagcagacga agacaccgta catggatgca ctcggcttcg
    28861 atctcacgga ggccgcccgc acggaccggc tcggtccgct cgttgggcga tcggtcgaaa
    28921 tcgaccgcgt gatgcagatt ctctcacggc gtacaaagaa caacccggcc ctcatcggcg
    28981 agccgggcgt cggcaagacg gcaatcgtcg aggggttggc gcaacgaatc gtctccggcg
    29041 acgtgcccga gcaactgcag aacaagcgcc ttgtcgcgct cgacatcggc gcgctcgtgg
    29101 cgggtaccaa gtaccgcggc gagttcgagg aacggctcaa gaagatcgtc aacgaggtca
    29161 aggagaccaa ggcaattctc ttcatcgacg agctgcacac cctcgtcggc gccggggcgg
    29221 ccgagggcgc cgtcgatgcg gcgaacatcc tcaagccggc actctcacgt ggagagctgc
    29281 agacgatcgg cgcgaccacg ctcgacgagt accgcaagta cattgagcgc gacgcggctc
    29341 tggagcgacg cttccagccg gtgcaggtca acgaaccgtc catcgaggag acaatcgaga
    29401 tcctgcgggg cgtgcgggca ctctacgaga cgcaccacga cctgaagatc agcgacgatg
    29461 cgctcggtgc ggcggcgaat ctcgcggcgc gttacgtcac ggaccgcttc atgcctgata
    29521 aggcgatcga cctgatcgac gaggctggct cgcgggtccg gatgcagcgc tcggctgcac
    29581 cgccgagcct caaggaggcc aaggcgggcc tgacggcgat ccagagcgag ctgggcgcgg
    29641 cggtcgacgg ccgggacttc gagctcgcgg ctgagctgcg ggatcgcgag cggaagctgc
    29701 gggaccgcat cgaggagcag gagcagaagc tcgcggatga gaagcagtcc gacgaggcct
    29761 acgtgacgga agaggatgtc gcgcaggttg tctccatgtg gaccggcatc ccgttggtgc
    29821 gcatcgccgg cgaggagtct gagcgcctgc tgcacatgga ggatgcccta cacgagcgga
    29881 tcatcggcca ggacgaggcg atctcgatga tctccaaggc cgtgcgccgt gcccgcgcgg
    29941 gcatcaagga tccgcaccga ccgatcgggt cgttcatctt tctgggtccg acgggtatcg
    30001 gcaagaccct gcttgcgcgg gcgctggccg aattcatgtt cggctcggaa gaccacctga
    30061 tcaagatcga catgtccgag ttcatggagc ggcattcggt cgcccggctc gtcggcgcgc
    30121 caccgggcta cgttggctac gaagagggcg gccagctcac ggaggccgtt cgacgcaagt
    30181 cgtactctgt catcctcctg gacgagatcg agaaggcgca cccggaagca ttcaacatgc
    30241 tgctccagat tatggaggat ggctcgttgg cggatgccaa gggccgccgg gtcgacttcc
    30301 ggaacacgat ccttatcatg acgtccaatg tcggcgccga ggagatctcg cgcgacctga
    30361 cgcttggctt cgcgattcag caggacgagg agatcagccg gcgtcgtgag ttcgacacga
    30421 tgcgcgagaa ggtgatgtcg cgcctgaagc agaccttccg gccggagttc ctgaaccggc
    30481 tcgacgcgac gatcgtcttc cactcgctca gcaaggagca gatccggcaa gtggtggagg
    30541 tcgagctcgc gcgggtgcgg aagcagcttg gcgagcagga gatcaccctt gacttcacgg
    30601 tcgaggcgat ggatctcatc ggcgaacgcg gctacgatca ccattacggt gcccgtccgc
    30661 ttcgtcgggt gatccagaac ctgatcgagg atgagctcgc ggagggcctg ctcaacggca
    30721 ggttcatgac cgggcagtcg atccgcgtcg acgtctcgaa ggatggcgag gcgctggagc
    30781 tcacaccggt cgaggagctt gctccggtat aggtcgaagc agtcgatcgc aacgcaatgg
    30841 gtgcagcgcc ggttcggtgg ttacgatacc accggccggc gctgcggtcg tatctggaag
    30901 agagcactgg cgtgccgaag cagcgaacgt catggatttg tcaggagtgc gggacctcga
    30961 gcccggccta tctcggtcgg tgcccgggct gcggcgcatg gcactcgatg gtcgaaaccc
    31021 tcgaccagcc cgcgcctgcc gcgaggatgc gttccacggc gcggcaggat ggcagcaggc
    31081 cggttcgcct ccacgaggtc gacgtctcga ccggtgggcg gattgcgctc agaatcgacg
    31141 agttcaaccg ggtgcttggg ggcggcattg tgcctggctc tgtggtgctt atcggtggcg
    31201 accctggcat cggcaagtcg acgctcatct tgcaggcagg tagcgatctg agctcgcggg
    31261 gcatgccggt actctacgtt tcggccgagg agtctgccca acaggtgcgg ctgcgggcag
    31321 accgcatggg tgttgatggc aaggacctct acattcacgc cgagattgac cttgactcaa
    31381 tcctcgcagc agccgagcag ctcaaaccgg ggttagtgat catcgactcg gtgcagacga
    31441 tccagtctgc cgagcttgaa tccacgccgg gaaccgtctc ccaggtgcgc acctgcgcca
    31501 gccggcttgc caactgggcc aagccgaaca acgttcccgt tttcctgatc ggccatgtca
    31561 cgaaggaggg aacagtcgcc ggcccgcgcg tgctcgagca catggtcgat gccgtgctct
    31621 acctcgaggg agaccggttc caccaatacc gcgtgttacg ggctgcgaag aatcggttcg
    31681 gctccacgaa tgagattggc gtgttcgaca tggccgattc ggggctgcaa gaggtgcgca
    31741 acccgtccga ggtgttcctg gaagagcgga acatgatggc ggccggctca gctgtggcgg
    31801 tcactctcga ggggtcgcgg ccgatcctgc tggagatcca ggcgcttgcc gcccaaacat
    31861 cctttgggct gccgcgacgg agcgcgaccg ggttcgacac gaatcgcctg cacctcctgc
    31921 tcgctgtcct gcaacgacgg gtggggatgg gactgggcaa tcacgacgtc tacgtcaatg
    31981 tcatcggcgg tttcagggtc aacgagccgg cggctgactt ggcggccgca gtcgcgatcg
    32041 catcgggcgt tcgggatacg cgggtgccgg ctgacacggt ctacattggc gaggtagggc
    32101 tctcgggtga tctgaggacg gtgcagcaca tcgaccgccg cgtacaggag gcggtccggc
    32161 tcggcttccg ccgggcggtg gtgccgggtc gctggaagga ggcggggcag aaggccggcg
    32221 ggattgaggt cacgggcatg cgcacgctgc gtgagacggt catggacatc cccggaatgt
    32281 ttggaggccg cgacctcgat gcggagaatg actaggagct ctctgcaagg gcgaggatct
    32341 ggtcgacgag ccgggaggcg gcctgcgggt tgccgaccgt cgtcgcggcc tcacccatgc
    32401 gcgctaggcg atccggatcg gcgagcagcg atgtgatgac ttccccaagg cgcgtagggg
    32461 ctgcgtcctt ctgtgcgatc acctcagccg cgcccgcgtt gctcaacatg cgggcgtttt
    32521 ttgtttgctc gtctccccaa gtgccgggca ggggaatgag gaccgatggc ttgccgaccc
    32581 gagcgagctc ggtaatggtt ccggcgcctg cacggccaag caccagcgag gccatggcgt
    32641 agacgtcggc gatctcgtcc cggataaatt cggtgacgtg gtaccggtgc tgcaatgcgt
    32701 ccggccaaga cgtgcgcagc ttctgcaacc tctcgacgtc cggattggcc gacggtggtc
    32761 cggcttggtg aaggatctgg gcgtgctcca gcaggccggg cagcagtgcc tcaactcgct
    32821 cgttcagagg gctggcgcca cgggcgccgc cggtgacgta gagaactggg aagtccggcg
    32881 agaagccgaa tcggcgcaga ccggcctctc gggacccgcc cagaatcgac gagcggaccg
    32941 ggttgccggt cacgacgaca cggttatgcc gctgccgggc gagcacggcc gtttcatcga
    33001 atgggaccgc gaacacgtcg gcgaagcggg acgccatcct gttggcaacg ccgatctgtg
    33061 ccgtttgttc gtgggtgatg atcggcgcca tgcgcgaccc ggcgaagacg gtcggaaagc
    33121 tgatgttgcc cccagtgccg aagatgacat cgggccggaa cgctcggaga gcctggaacg
    33181 cctgcgcgat accaagtggg atgcgcagca tatcggtggc ggtttggata gcgaggtagc
    33241 gacgcagctt gccggtctga atggcgacga acgcgatgtc gttcgcctcg gccagctcac
    33301 gctctacgcc ggtgtggccg ccgatccaaa ggagttccaa ggggaccatc cggcggcgca
    33361 actcctccac caccgccacg gcgggcagga cgtggcctcc cgtgccgcct ccggctatga
    33421 cgaggcggag aggcctttcc ttcgggcggt cggcatcgat cattgagtgt gctcctctcg
    33481 ggcagcgtct gctgttccgc cggacagtat agccgccgat cgaagcgcgc caccgtgata
    33541 cgcttgcgga gaaggagttg gcccgcccgt gcgattcgcg ccccctatga ccttgccaga
    33601 tctgcttgcg cgcgtggccg ccgtcatggc ggtcgtggtt gtgctcgatc tcctcgtcaa
    33661 gtgggccgtg acgctctgga tcggtccgga cgccgatcgc cactcttggt ggctgattgg
    33721 ggactctatc gggtttgagt atctgcgcaa cacgggggcc gcctttggac tgtttcgggg
    33781 caatcccgag ctgctggccg ccttcacgct gttggtcacg gtcggtattg tccggctggt
    33841 gctgcttgag gtgcgatcac cgttctgggc ggcgctttcc agcgggctgc tcgctggcgg
    33901 tgcaatcggc aatttcgttg agcgagtgat cagcggctac gtgaccgact tcgttgccgt
    33961 cggcccgtgg ccgcggttca acatcgccga ttccaccatt acgatcggta tcgcgatctt
    34021 cgccctcgcg gttatcttgc cggacaaatt tggcgcatcc gatcaggcaa cagcggagga
    34081 gggtgtacgt gacagacgag cctgagatca acgactcggc cgacatggcg tacttcgagc
    34141 tgagcccgga catcagccag cggaacgagc ggctcgaccg gtatatcgcc agcgagctgc
    34201 caaatctgag ccgctccggc gtgcaggcgc tgatcgaagc agggcatgta ctggtggact
    34261 gccagcggcg caaggcgtcg ttcaaggtga cgccgggcga ggtcgtcgcg gtttcggttc
    34321 cgcccctggc ccccgacgac gtcgagccgg aggacatccc gctcgagatt ctgtacgagg
    34381 atgccgacgt gatcgtgatc aacaaggcgg ctggcatggt ggtacacccc gcgcccggac
    34441 acgcctccgg caccctcgtc aatgcgctgc gctttcatgc aatcgagatc gcggagctcg
    34501 actcgagccg caccggcatc gttcaccgcc tcgacaagga cacctccggg gtgatgatcg
    34561 tcggaaagca caaccgcgcc gtccagttcc ttcaggacca gtggctcgac ggcagcgtcg
    34621 acaagcgcta cgtcgcgatc gcatttcgcg cttttgcaga ggaggaggcg gtgatcgatg
    34681 tcccgatcgc tcgccaccgc accgagcgca aacggatgga cgctggtcgc gagggacggg
    34741 aggccctgac catcgcgagt gtccgcgagc gctttccgaa ggcgacgctg ctcgatgtcg
    34801 acctccaaac cggtcgaacg caccagatcc gcgtgcatct cgcattcatc aagcacccgg
    34861 tcctcgggga cacggtctac ggcactcagt catcgaccgc gtactcccga gaattgggag
    34921 cgaggcgcca aatgctgcac gcggcgtcgc tgacggtaga gctacccgta ggcggcggtc
    34981 cccgaacctt caccgcgtcg ctgccgttcg acattcgggg agtgctggac aagctgcgcg
    35041 ccatgaagga gcaagacgat ggttgacctg agtgaccgga gtgcatgccg gtcgcttgtt
    35101 gaacgcgcct acagtgcagg aatcgccaga gtcgcaccgg atgaagccgt tcgccgcgta
    35161 ttgcgtcgtg acggcgccgc actggtggtc gacggtgagc ggataccggt atcgggccgg
    35221 ctggtggtgg ctgcaatcgg caaagcggcc acgcccatgg cgaccgctgc cgccgagacg
    35281 gcgggtgacc tgatcgatca ggggttcttg ctgaccaagg aggggcatgc ggagcagccg
    35341 ccggcaggtt tggaggtctt cgaggcgtcc caccccgtcc cggatgagcg gggcatcgaa
    35401 gcgtcccggc gaatcctaga agccgtcgga gggctcggcg cagacgacgt ggtggtcgcg
    35461 ctgatctcgg gcggcggttc ggcgctcttc gaggtgccgg cggatggtct gacgcttgac
    35521 gacattcagc agtcgaccag catcctcctg cgcgcgggag cgccgatcca agacctcaac
    35581 gctgtgcgcc aagagttgag ccgagtgaag gggggagggt tccggcgggc gatcggcgat
    35641 gcgtgcacca tctcgctgat tctgtccgac gtgctgggca acccgccgga gatcattgcc
    35701 agcgggccga cagtcccgcg cacaacgaac cccaaagcgg ggctcgacgt gctggaccga
    35761 tatggcgtgc ggaacgccgt tccatcggcg gtggttcggc atcttgagcg gactggcgag
    35821 cgacccgacg cgccacaggc aggtaagcgc ggtctctacc gcgttgtcgg tgacaatgag
    35881 gcgttcgtcg cggcggtcga ggacttcttt gcccaggaag gactgactac ggagcgagtg
    35941 tggcacgagc gcgagggcga ggcgcgcgaa caggcccatg cgtgggtcga ggcgcttgag
    36001 agctcagcgg cggacgtcat cctcggtgga ggggagatga ccgtcacggt gcgcggcgac
    36061 ggcgccggtg ggcggaatac cgagtttgca ctcgcagcgg cgataaatct cgaagccaag
    36121 cgcctcaccg cgactgttgc cagcctggct tcggacgggc aggatggaag cattgatgcc
    36181 gccggtggga tagtggatga gcttaccgtc cgcgagctgc gcgaggccga catcgagccg
    36241 gctgatgcga tggagcggag cgacagcggc ggcgcgctcg ccgcgatcgg ggctctgctc
    36301 gtcccgggtc ctaccggaac gaacgtcaat gatgtctaca tcgggttgag acacgcgatt
    36361 tgacacgccg gtctacgaag gggattggga accaatgaac ggtgcagaac tgctcgtgca
    36421 aaccctccga gcgcatggtg tggagcatat gttcggcctc ccgggggata cgggtatggc
    36481 gttctatgac gcgctatacc acgagccggc gatcacgcat gtgatgacac gcgacgagcg
    36541 ttccgcgtcg tttatggcgg acgtctacgc gcgcgtatcg taccggctcg gtatctgcga
    36601 gggtccgagt ggcggtgggg cgacctacag cgtgcccggg gtggccgagg cccatggctc
    36661 cgccgtgccg gttctctgcc tgacctccga cacgccagtg tcccagcagc actggaatgt
    36721 gctgacagag ctcgaccagg tggcgctgtt ccgccccgtc accggttgga cggaacgcgt
    36781 gctggcggcg gactcgatgg acgatgccct tcgtcgggcc attcgcatgg cgatttccgg
    36841 tcgaccgggc gcctcgcatc tgtccatgcc cgaagactcg ctcgagaatg acgtcttgga
    36901 gagagtcgcc cgccaaagtg acgagcgtct caccgatcgg ttccccctct cccggccgcg
    36961 cgccgaccgt gccgaggtgg accggtcggc tcgcctgctg gtcgaggccg agc
//
LOCUS       DASYWL010000164         2111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_420790, whole genome shotgun sequence.
ACCESSION   DASYWL010000164 DASYWL010000000
VERSION     DASYWL010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420790"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VGR22_05955"
     CDS             <1..281
                     /locus_tag="VGR22_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128145.1"
                     /translation="LVPRTLNTPAVDQTWEATFEPRPRRGLVTDVTLEIPPLATEGTG
                     DYLLEITVHDGETGESLPVYPPDSSISHPAAVIGSRCASGVPDAGANP"
     gene            762..>2111
                     /locus_tag="VGR22_05960"
     CDS             762..>2111
                     /locus_tag="VGR22_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128146.1"
                     /translation="MLLLLVLVAVAGLYVWPRTLAIDRVVTVDEPVFLGISANFYHAL
                     AHGDLAETSQFLYPAVTIMWAGTLGYLAELPNYVTDDPGPIEAPTWGEGRLKSVHEPI
                     RAAGGDPLSVLIAARVAKIVLQAGVFLVAVWLMYRLFGLAVTALAAAFIVFDPFLVAH
                     DQLLHVDGMTGITAFASMLAAAYADRYPARLWLWALAGVLAALCWLTRLTGLVLLPII
                     LLVIIDGAISRYRQRTQPGRAAALTAAKTAALVVGASLVTTVVLWPALWVRPVGTIAE
                     TLAEWRRSIETPHPWGLYFAGETVEGDPGILMYVFVALYKLTPFTLVGLAIVALAFLF
                     RIASIMPRRSWRPVVILAAFVVVYSVGMAAGTRKFDRYILPDFVFLDLFAAIGIVGTA
                     RLIWATGRIAWKRVATIVIVGLVAGQMALALAQRPYLLDYYNPLLGGTQTAEEMLMLG
                     "
BASE COUNT          316 a          717 c          689 g          389 t
ORIGIN      
        1 agctggtgcc gcgcacgctc aataccccgg cagtcgacca aacatgggaa gcgaccttcg
       61 agcccagacc acggcgcgga ctagtcacgg atgtgacgct ggagatcccg cccttggcca
      121 cagaaggcac cggcgactac ctgctggaga tcaccgttca cgacggcgag accggggagt
      181 cgctcccggt ctacccgccg gacagctcca tcagccaccc agcggcggtg atcggctcca
      241 ggtgtgcaag cggtgttccg gacgcgggcg ccaatccgta agctccggtt ctgtcctgcc
      301 gagggggcct ggggcgtatg tcctcgtcgc ggcggtgatg acccggcccg gccgggcccg
      361 acaggccgac cgctgtgatc actggcggtc gttgcctccg atcaagcggc acgtgcgacc
      421 ggtgctggtc attggagctc cccggccaat ccctacggag atggatataa tgtgcgcacg
      481 cccacgcgaa gagacgaagg gcaaaccacg agctggagca ggtgaggtgt gttccaggac
      541 gtgcgatggt ttgcacctcg acggaaaccg cgcccggcat tcgaggtggt gtgtgtatgg
      601 atctcggcgc cacggactcg tcccagccgt tcacggaccg ctccggcctc agcgaggggg
      661 caacgtcaac gcgggcagag accactccgc ccgccagacc ccagacacca tcccggcccc
      721 gggcgagttt cggccgggat ctccctcgag ctcgacggcg cgtgctgctt ctgctcgtcc
      781 ttgtcgccgt agctggtctc tacgtgtggc cgcgcacgct cgccatcgac cgcgtggtaa
      841 cggtcgatga gcccgttttc ctcggcatct cggcgaactt ctaccatgcc ctggcacacg
      901 gcgatcttgc cgagacgagc cagttcctct atcccgccgt gacaatcatg tgggccggca
      961 cgctggggta cttggccgag ctcccgaact atgtcacgga cgatcccggc ccaatcgagg
     1021 cccccacctg gggagagggc cgcctcaaat cggtgcacga gccgatccgc gccgccgggg
     1081 gcgatccgct ctcggttctc atcgccgcac gtgtggccaa gatcgtcctg caggccgggg
     1141 tgttcctcgt cgccgtgtgg ctgatgtatc gcctgttcgg ccttgccgtc accgcgctcg
     1201 ccgcggcatt catcgtgttt gacccgtttc tggtcgccca cgatcagttg ctccatgtcg
     1261 acggcatgac cggtatcacc gccttcgcga gcatgctcgc cgctgcgtac gccgacaggt
     1321 atccggcgag gctatggctc tgggcgctcg ccggggtgct ggcagcgctc tgttggctca
     1381 cccggctgac cggcctggtg ctgctcccga tcatcctgct cgtgatcatc gatggcgcga
     1441 tctcccgcta ccggcaacgc acacagccgg ggagagcggc agcgctgacg gcggccaaga
     1501 cggccgccct cgtggtcggg gcgagcctcg tgaccacggt cgtgctctgg cccgcgctgt
     1561 gggtccgccc ggtcggcacc atcgcggaaa cgctggcgga gtggcgccga tcgatcgaaa
     1621 cgccacaccc gtggggcctg tacttcgcgg gagagacggt cgagggcgac cccggtatcc
     1681 tgatgtacgt gttcgtagcg ctctacaagc tcaccccgtt caccctggtg ggcttggcca
     1741 tcgtggccct cgcctttctg tttcgcatcg cgtcgatcat gccccgccgc tcctggcgac
     1801 cggtcgtgat tctggccgcc ttcgtggtgg tctattccgt ggggatggcg gctggaacgc
     1861 ggaagttcga tcgctacatc ctcccggatt ttgtgttcct cgacctgttc gccgccatcg
     1921 gcatcgtcgg cactgcccgc ctgatatggg cgacggggcg catcgcgtgg aagcgcgtgg
     1981 cgacgatagt gatcgtgggc ctggttgccg gacagatggc tctggcgctt gcgcaacgac
     2041 cgtatctgct cgattactac aacccgctgc tcggaggaac ccaaacggcc gaggagatgc
     2101 tcatgctcgg g
//
LOCUS       DASYWL010000165         2319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_44971, whole genome shotgun sequence.
ACCESSION   DASYWL010000165 DASYWL010000000
VERSION     DASYWL010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44971"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1324
                     /locus_tag="VGR22_05965"
     CDS             <1..1324
                     /locus_tag="VGR22_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2128147.1"
                     /translation="DGTQTWTVRLRDDVMVHPSETAGTESYQLTAEDVVFSFQKAANP
                     DTSSYSANYEGWSFEVVDELTVEIILDQPISETLFYPNIANYSGGFIIPKVPFEALGA
                     DGFVTAPVGTGPFMLDSYTTQNSVVLDAFDPFYRGAPELAGVEVRFIPDATSRELALL
                     SGDLDVIHGLPEAQWVERIDAMEGFAADVFGVGEVVFLNINTEVEPFDDPLVREAVIT
                     ALSRENHIALSGEPVGEAVFSPVPAQLMPGGLTEEEANEAGVNIETNPEEARALLEEA
                     GYPDGFEVDLVTSEQDAYRRNYEVLAEELRQIGINVNLEVVQHQAYHDLIRQDENALT
                     FYIAYRPNAHVYLSQFFTEGGGPTNFSKFDVSEYVDQALATTDPDEQAQLWKEANIAL
                     QENYAGYGLYLVNQVYARAEYVDYGHELTAVVNLYPGITEKTSTSNGS"
     gene            complement(1430..1819)
                     /locus_tag="VGR22_05970"
     CDS             complement(1430..1819)
                     /locus_tag="VGR22_05970"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2128148.1"
                     /translation="MSMRPDGGGRIMMRDWDFDAMADAATPAHPHPPFVHDLLDRVVT
                     VLPTLAGARVEAARIAVRPIPGDDLPVVGYVPGVDGLYVMVGHGAITHGPLVARLAAH
                     EIAFGECCARLEPYRPERLVTPLTSVG"
     gene            complement(1867..>2319)
                     /locus_tag="VGR22_05975"
     CDS             complement(1867..>2319)
                     /locus_tag="VGR22_05975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2128149.1"
                     /translation="WLHVSGHVEWDMAEGGPDRIQRKMVDLHSWGYSAELLPVGDLAS
                     LEPDLTPPVGLEAFIYYPNEGYIDPAAFVGLLANRARELGTTIRTGVAVTGLLQERGR
                     VTGVVLASGERIEADTVVACAGIGAPGILEQVGMHLPMLHSVRWAWWR"
BASE COUNT          418 a          801 c          717 g          383 t
ORIGIN      
        1 tgacggaacg cagacatgga ccgtacggct gcgcgatgat gtgatggtgc atccgagcga
       61 gactgcgggc acagagtcgt accagctgac tgccgaagat gtcgtcttct cgttccagaa
      121 ggcggccaac ccggacacct cgagctactc ggccaactat gagggctggt cctttgaagt
      181 ggtcgatgag ctgacggtcg agatcatcct tgaccagccg atctccgaga cgctcttcta
      241 cccgaacatc gccaactact ccggcggctt catcatcccg aaggtgccgt tcgaggcgct
      301 gggtgcggat gggttcgtca ccgcgccggt cggcaccggc cccttcatgc tcgatagcta
      361 tacgacgcag aacagcgtgg tgctggacgc gttcgatccg ttctaccgcg gcgcgccgga
      421 gctcgccggc gtcgaggtgc gcttcatccc ggacgcgaca tcgcgcgagc tcgccctcct
      481 gtccggtgac ctcgatgtca tccacgggct ccccgaagcg cagtgggtcg agcgcatcga
      541 cgcgatggaa ggcttcgccg cggacgtctt cggtgtcggc gaggtggtct tcctcaacat
      601 taacacggag gttgagccgt tcgacgaccc gctggtccgc gaggccgtga tcacggcgct
      661 cagccgcgag aaccatatcg cgctctccgg cgagcccgtc ggcgaggcgg tcttctcccc
      721 tgttcctgcg cagctcatgc cgggtggcct gaccgaggaa gaggccaacg aggccggtgt
      781 caacatcgag actaacccgg aggaggcaag ggcactccta gaagaggctg gctacccgga
      841 cggcttcgag gtcgatctcg tcacctcgga gcaggatgcc taccggcgca actatgaggt
      901 cctcgccgag gagctgcgcc agatcggtat caacgtcaac ctcgaggtgg tgcagcacca
      961 ggcctatcac gatctcatcc gccaggacga gaacgcgctg accttctaca tcgcctaccg
     1021 gccgaacgcg catgtctacc tttcacagtt cttcacggag ggtggcggcc cgacgaactt
     1081 ctcgaagttc gatgtgagcg agtacgtgga ccaggcgctc gctaccacgg atcccgacga
     1141 gcaggctcag ctctggaagg aggccaacat tgccctccag gagaactacg cggggtacgg
     1201 cctctacctg gtgaaccagg tctacgcgcg cgccgagtac gtggactacg gccatgagct
     1261 caccgcggtg gtcaacctct acccgggcat cacggagaag acctcgacga gcaacggcag
     1321 ttagcgccgc agctggcgac cgatccgcca gcgcgccttg ctgcgagccc gcccggtgtc
     1381 tagccgggcg ggctcgtcat ttcgctcccg tcgtgcatcc ggctcctgat cagccgacgc
     1441 ttgtcaacgg cgtgacgaga cgctcggggc gatatggctc caggcgagcg cagcactcgc
     1501 caaaggcgat ttcgtgcgct gccagccgcg ccaccagcgg gccatgcgtg atcgcgccgt
     1561 gcccgaccat cacgtaaagc ccgtccacgc cgggaacata cccgaccacc gggaggtcat
     1621 cgccggggat gggcctgacc gcgatgcgcg ctgcctccac ccgcgcaccg gcaagagtcg
     1681 gcaacaccgt gacgacccga tccaggaggt catggacaaa cgggggatgc gggtgtgccg
     1741 gcgtggcggc atccgccatc gcgtcgaaat cccaatcccg catcatgatc ctgccgccgc
     1801 cgtcgggacg catgctcatg ccctcgttgt gatgcaccgc tcgcaatcgg gctggtgatg
     1861 gcccggtcac cgccaccagg cccaccgaac cgaatggagc atgggcagat gcatcccgac
     1921 ctgctccagg attcccgggg ccccgattcc ggcacaggca acgaccgtat cggcctcgat
     1981 ccgctcgcca ctcgcgagca ctaccccggt gacgcggccc ctctcctgca gtagccctgt
     2041 caccgccaca cccgtccgga tcgtcgtgcc cagctcgcgc gcccggttcg ccagtagccc
     2101 gacgaacgca gcggggtcga tgtacccctc attcgggtag tagataaagg cttccaaccc
     2161 gaccggcggt gtcaggtccg gctccaggga cgccagatcg cccaccggga gcaactcggc
     2221 gctgtagccc cagctgtgga gatcaaccat cttccgctgg atacgatccg ggccgccctc
     2281 tgccatgtcc cactcgacat gtccactgac gtgcagcca
//
LOCUS       DASYWL010000166         2406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_551870, whole genome shotgun sequence.
ACCESSION   DASYWL010000166 DASYWL010000000
VERSION     DASYWL010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551870"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..232)
                     /locus_tag="VGR22_05980"
     CDS             complement(98..232)
                     /locus_tag="VGR22_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128150.1"
                     /translation="MLVVAFICFLSLIVGWLVAPTSAPRQSVEPTSSAAVSEPGTAPA
                     "
     gene            complement(289..1500)
                     /locus_tag="VGR22_05985"
     CDS             complement(289..1500)
                     /locus_tag="VGR22_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018284512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEV2128151.1"
                     /translation="MDTGNTAFVLIAAALVMLMTPALGFFYGGLVNRKNVLSTLMHSF
                     FTLALVSVVWVLWGYSLAFGEDKGGLIGGLDFLGLRGIAPDDLIEGPDIPVMAFVGFQ
                     MMFAVITPALITGAFAERKRFKSFVIFTLAWSTLVYAPLAHWVWGGGWLFEMGALDFA
                     GGTVIHISSGVAAVIAARMLGPRVGFGSKPADPHNLTLTVLGAGLLWFGWFGFNAGSA
                     LAANGLAAHAFVVTNVAAAMGAMTWMTVSWIHHGRPSVLGAAAGAIAGLVGITPAAGF
                     VGASSAIIIGFGAGIFCFFAVDLLKQVIKLDDALDVFAVHGVGGIWGALATGLFADAA
                     IGGTDGLFFGNPEQLGIQFVATVAAIAFAAVMTFLILKAIGLVTALRVQEDEETLGLD
                     SSQHGELAYQL"
     gene            2028..>2406
                     /locus_tag="VGR22_05990"
     CDS             2028..>2406
                     /locus_tag="VGR22_05990"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV2128152.1"
                     /translation="MIEGQKIYGRADISEVIASIPPDPNSEARHRANTLIKTSTLRVV
                     VVTMLEDGELQEHTAPGPITIHTIEGSMEVDVEGEAHPLDAGELISLAPGVRHAVRCT
                     RDGAFLLTIGVFLREPDPGGRHGG"
BASE COUNT          523 a          751 c          775 g          357 t
ORIGIN      
        1 ggcgcccacc acactgtgga atcagccaac actgaagccc gccccgtagg acgagctcca
       61 gcgcgcagga ggatgaaaat gaggtgtgat cacattgtca ggcaggcgcg gtgcctggct
      121 ccgagacggc ggcagaactc gtcggctcga cgctctggcg cggcgccgag gtgggagcga
      181 ccagccatcc gacgatgaga ctgaggaagc agatgaaggc gacgaccaac atggtgaggt
      241 tcctttcctt cgaaacctga gcggacgccc actgggatga cggtgtcgct agagctggta
      301 ggccaactcg ccgtgctggc tcgagtcaag tccgagggtt tcctcgtcct cctgcacgcg
      361 aagggcagtc accaggccga tcgccttgag gatcaggaag gtcatcacgg cagcgaaggc
      421 gatcgcggca accgtcgcga caaactgaat cccgagctgc tcagggttgc cgaaaaagag
      481 accgtctgtg ccgccgatgg cggcgtcggc gaagagtccc gttgccaacg cgccccagat
      541 accgccgact ccgtggaccg cgaagacgtc gagcgcgtcg tcgagcttga tcacctgctt
      601 gagcaggtcg accgcgaaga agcagaagat gccggcaccg aagccgatga tgatcgccga
      661 ggaagcccca acgaatccgg cggccggggt gatgccaacc agaccggcga tcgcgccagc
      721 tgcggcaccg agcacgctcg gacgtccgtg atgaatccag gagacggtca tccaggtcat
      781 cgcgcccatc gccgcagcta cgttcgtgac tacgaaggcg tgcgcagcga gcccgttcgc
      841 ggcgagagcg ctgccggcgt tgaagccgaa ccagccgaac cagagcagtc cagcgccaag
      901 gaccgtgagc gtgaggttgt gcgggtcagc cggtttgctg ccgaatccga cacgcgggcc
      961 gagcatccgg gcggcgatca ctgccgcgac acccgaggag atgtgaatga cggtgccacc
     1021 ggcgaagtcg agggcgccca tctcgaagag ccacccgcca ccccagaccc aatgggccag
     1081 tggcgcatac accagcgtcg accacgccaa ggtaaagatc acgaaggact tgaagcgctt
     1141 gcgctcggcg aacgctccgg tgatgagcgc cggggtgatg acggcgaaca tcatctggaa
     1201 gccgacgaag gccatcaccg ggatgtcggg accctcgatc agatcgtccg gtgcgatgcc
     1261 gcgcagcccg aggaagtcga ggccgccgat cagcccgccc ttgtcctcac caaaggcgag
     1321 tgagtagccc cacagcaccc acaccacact gacgagcgcc agcgtgaaga agctgtgcat
     1381 gagggtcgag agcacgttct tgcggttgac cagaccccca tagaagaacc ccagcgccgg
     1441 ggtcatgagc atgaccagcg cagccgcgat cagaacgaaa gccgtgttac cggtgtccat
     1501 aggaaaactc ccctccatgc ttggcttgcg gtgtcaccct ccgaacaccg cgtcaactgt
     1561 gcactctgcc caacatcggg cagtgcgact gcgctgtcgc tcaatggatt cagtgtgaag
     1621 cgctgctcga gcggatgtaa gggacaagcg gcacagatcg agcgggaatt cttgtgcaat
     1681 tcggcacacc acgagatcgg aacatcctgt tggacagtga agttggcatt gagatcgacc
     1741 tgccgggcag cgatcgagcc ggtcgccccg gggatggcac tggctggcga accgttcaac
     1801 gcgaacagga tctccgcgca aacaaacatg caactccgct cgatgacccc gtgaactcgc
     1861 atccccgcac ctcttgtgga atctgtcgag cgcatcaaac tgcccctgct acagttgctg
     1921 cgactggtat cgggattgcg attcagggcg atagcaagtc ccgtgtaggc tgtacgcgaa
     1981 acccagaatc gggcaagatg gcgccagcca gttgaggagg agacaccatg attgaaggcc
     2041 agaagattta tgggagggcg gacatctccg aggtgatcgc gtcgattccc ccggacccga
     2101 acagcgaagc gcgacaccgg gccaacacgc tgatcaagac gagtacgttg cgcgtagtgg
     2161 ttgtgacaat gctggaggac ggcgagctgc aggagcacac cgcaccggga ccgatcacca
     2221 tccacacgat cgagggcagc atggaggtcg acgtcgaggg cgaggcacac ccgctcgacg
     2281 ctggtgagtt gatctcgctc gcgccgggcg tccgccatgc cgtccgctgc accagagacg
     2341 gggccttcct gctcacgatc ggcgtattcc tgcgcgagcc cgatcccggt ggccgccacg
     2401 gaggca
//
LOCUS       DASYWL010000167         9335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_307773, whole genome shotgun sequence.
ACCESSION   DASYWL010000167 DASYWL010000000
VERSION     DASYWL010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9335
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307773"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1575)
                     /locus_tag="VGR22_05995"
     CDS             complement(<1..1575)
                     /locus_tag="VGR22_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEV2128153.1"
                     /translation="MQTETHPLLADLNDDQQRAVTTIEGPVLVVAGPGSGKTRVLTHR
                     IAWLMEEEHVPPEGILAVTFTNKAANEMRDRIRRLVSGAAADGLVMGTFHSVGVRILR
                     QNPYRAEQLINLKPNFVIYDTADQLDVAKEAIIHERMDPKQIAPRRMLSGISRLKSEM
                     KTPQDAAAEAMSYDDELVARVFAEYEHRLRDLNAVDFDDLLTLPLRLFDADPQLLEKY
                     QAQFRHILVDEYQDTNRVQYVIVTALADIWRNLFVVGDPDQSIYAWRNADITNILNFK
                     DDYPDAKQINLELNYRSTGYIVQAADRLIRDNTDRIDRPIRTMNPDGEKIVLRELADQ
                     NHEASFVVDEIRRLTQQGSAHPEDIAVMYRTTAQSRVLEEAFRRSEIPYRIIGGVRFY
                     ERREVKDVLAFFKLLYNPDDRLSLDRIIDNTPLGKGFGPKAMETLLDWSRDAGLSPVD
                     GLIALAPSVAQDQRLASPPEITGSARKTAERIGSTLVILLERSTSSTLSELFDDIVEV
                     SEYREQFRNGTEEELQR"
     gene            1721..1795
                     /locus_tag="VGR22_06000"
     tRNA            1721..1795
                     /locus_tag="VGR22_06000"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1753..1755,aa:Met,seq:cat)
     gene            1811..2872
                     /gene="tilS"
                     /locus_tag="VGR22_06005"
     CDS             1811..2872
                     /gene="tilS"
                     /locus_tag="VGR22_06005"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEV2128154.1"
                     /translation="MNRRFHDDQSRAPEFLRGESGLIQRVRRFLRDHFRGEAPHLVVG
                     YSGGQDSLALLLVLGELERLGACRVTAAHVDHGLRPEAAQDAERVVEIGCALGVDVRV
                     YRPETPLREQYRGRSVEDAARVFRYRTLASVVSTVNADAIAVAHHARDQAETVLLHVL
                     RGSGLAGLSGMAADKVLPVPGSMSGVEMRVIRPFLHESPETLVELVERSGLPIIEDPS
                     NTSPDFRRNRIRHALLPLMEDIAPGATGRLVSLADILRADEEALDDVSHLLLHRTTDD
                     ASRLVWDPIQGAPVGLQRRVVRHWILQSGFSGDLSRERIEAVVELTRRNEGGKQIEIG
                     GGWSVRYAEGRLELRGRSH"
     gene            complement(2962..6321)
                     /locus_tag="VGR22_06010"
     CDS             complement(2962..6321)
                     /locus_tag="VGR22_06010"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004225.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEV2128155.1"
                     /translation="MYTELHLHTSFSFLDGASQPDELVRRAQEFGYTALAITDHDGLH
                     GALEFAQLAEEAGIQPITGAEITLADGSHLTLLAETPTGYANLCRVLTHLHHGRHPAP
                     VALSDDPEPSPGNPPLGDYAEGIILLTGCRKGQLSRVVGDNNMRAAIQILDQYIAWFG
                     HDNVFVELQQNEVFGDTVRIRALVDLANAIGLGCVATGNVHYHTAVRHRLQDVLVAIR
                     NHTTLDGCHRERRPNARFALPTHMEMERRFAEWPEAIANTQVIAERCEAFRITRDLKY
                     TFPAYDAGTGETPDETLARICWETFPQRYSEDNHRAREQIREELRLISRHNLAGFFLI
                     YRDLLELAREVAAELRGGPISGSRRFLPPGRGRGSSVSSIVCYLIGLSPVDPLAHDLY
                     VGRFLNEEMPSIPDIDLDFPREIRERLIERVYDVYGADHAALVCAFSTYRLRSAVRDV
                     GKALGLPLADLDRISRLSEPRSADSLAEELNHLPEYAARRNTPPWSYLIELAKQLAGF
                     PRHVTQHSGGMIVSATPLNEIVPIQPAAMEGRFMCQWDKDSCEDARFIKIDFLALGML
                     SLVEEVLDLIDESGANPVDLSRIDFNDPAVYRMIQAGDTFGVFQIESRAQIQMLRRTL
                     PARLDDLVVQVAIVRPGPIVGGAVTPYVQRRENPDYIPEYDHPRLEPLLKETLGVILY
                     QEQVVQVAEELAGFTPGQADQLRRAMTRKRSAEAMEVMRSTFIGGALGQGVDHKTAEA
                     VFDKLTGFAEYGFPKSHAAAFALLAYQSCWLRHYYPAEFLCALLNNQPMGFYAPHVLV
                     NDAKRHGIPVLRPDVNESEVACTVAGRRSVRIGLAFVKGLGEDVATAILLERLAHGPY
                     RSLADLIRRVPLRQEAVRHLIAAGACDGFGMHRREMIWQLGLFIPSRALTKGRKHKTE
                     GRQLSLALPTEQDAVELPRTSAWERMAGEYRILGMSTTFHPMALLRPRLPKDMVSTRN
                     LRDLPDGAPIRLPGLIVCRQRPGTAKGITFLLLEDEFDLVNIVVHPWLYEKQRHHVRA
                     TPLLVVHGRLQHAKNNINVVAESVQPIEDMQLVYPYDGHDPRSGHPLEEPREEVDPRV
                     IRIIALREERTEGPRRRADIRSISPDSHNYR"
     gene            complement(6609..6737)
                     /locus_tag="VGR22_06015"
     CDS             complement(6609..6737)
                     /locus_tag="VGR22_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128156.1"
                     /translation="MIDDEWWRDPISRQYFQVVLEGGIVRTIFHDRTNDTWFTQNY"
     gene            complement(6839..8032)
                     /locus_tag="VGR22_06020"
     CDS             complement(6839..8032)
                     /locus_tag="VGR22_06020"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEV2128157.1"
                     /translation="MSIACLHVPHFGLRVALLAQPELDGHPLILSDPHHGHPSVLDAT
                     PEARQKGIRTGLSLREASALVPNAIIIASNPATEHEQLHAITAALDTISPLVQPDAGD
                     PGTWYIDLTGLGRHYDTTLEAGEHLLRQVDSGLRPRAGVAVGKFAARVAANRSTPGTV
                     SLVPPARTQAMLAGAPVELLPLDAGTIHELHLMGIETLGQFVAIPRARIGARFGPAGQ
                     HARDLVTGDANDPVEPPARELTVAEYLELPAPATSHDVLMFALKQLVHRAFRSPDLRY
                     RHARGITMRAGLEHSRSWQRSMVFKDAYGPDWLVQAIGLRMQHLEMPGPVESLTVEIQ
                     GIVETSAHQAMLPALRPRHLAPLSHAVSMLKQRYGLSPLFRIVEVEPWSRIPERRHAL
                     VSFDP"
     gene            8586..9179
                     /gene="hpt"
                     /locus_tag="VGR22_06025"
     CDS             8586..9179
                     /gene="hpt"
                     /locus_tag="VGR22_06025"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEV2128158.1"
                     /translation="MSEGNQAMAVPAEARAEGVARVLISEHDLQQEIQLIAHELDEAY
                     GDDRPLMIGVLKGAVAFMADVLKAMTIPVEIDFMAVSSYGASTQSSGVVRILKDLDQE
                     IEGRRVIIIEDIVDSGLTLQYLLDLLERRSPKDIRVVALLKKIKSDAAQVQVDRVAFE
                     IPDEFVVGYGLDYAEGYRNLPYVAVLDPSVYSEPDGG"
BASE COUNT         1574 a         2742 c         3304 g         1715 t
ORIGIN      
        1 gcgctgcagc tcctcctcag tgccgttgcg gaactgctct cgatactcgc tcacctcgac
       61 gatgtcgtcg aagagctcgc tgagtgtcga cgacgtcgat cgctccaaca ggatcaccag
      121 cgtcgagcca atgcgctcgg ccgtcttgcg cgccgatccg gtgatctccg gcggtgaggc
      181 gagccgctga tcctgcgcga ccgacggcgc gagtgcaatg agaccgtcga ccggcgaaag
      241 cccggcgtcc cgcgaccagt cgagcagcgt ctccatcgcc ttcgggccga agcccttgcc
      301 gagcggggta ttgtcgatga tacggtcgag gctgagccgg tcgtcggggt tgtagagcag
      361 cttgaaaaag gcgaggacat ccttgacctc gcgccgctcg tagaaccgga cgccgccgat
      421 gatgcggtag gggatctctg agcgccggaa tgcctcctca agcacgcggg actgtgccgt
      481 ggttcggtac atcaccgcga tgtcctccgg gtgcgcggac ccctgctgag tgaggcgacg
      541 aatctcgtca acgacaaagc tcgcctcgtg gttctggtcg gccaactctc gcaggacgat
      601 cttctcgcca tccgggttca tcgtgcggat ggggcggtcg atccggtcgg tgttgtcgcg
      661 gatcagccgg tcggctgcct gaacgatgta gcccgtggaa cggtagttca gctccaggtt
      721 gatctgcttc gcgtcggggt agtcgtcctt gaaattcagg atgttcgtga tgtccgcgtt
      781 ccgccacgca tagatcgact ggtccgggtc gccgacaacg aagagattgc gccagatatc
      841 ggcgagggcg gtcacgatca cgtactggac gcggttggtg tcctgatact cgtcgacaag
      901 aatatggcga aactgcgcct ggtacttctc gagcagctgc gggtccgcgt cgaagaggcg
      961 gagcggcagg gtgagcaggt cgtcgaagtc gacggcgttg aggtcgcgca gccggtgctc
     1021 gtactcggcg aagacgcgcg cgaccagctc gtcgtcgtag gacattgcct cggcggcggc
     1081 atcctgcggc gtcttcatct cggacttcaa tcgggagatg ccgctcagca tgcggcgtgg
     1141 cgcgatctgc ttgggatcca tccgctcgtg gatgatcgct tccttggcga catcgagctg
     1201 gtcagcggtg tcatagatga cgaagttcgg cttgagattg atgagctgct cggcgcggta
     1261 cgggttctgg cgcaggatgc gcacgccgac cgaatggaag gtccccatca cgaggccgtc
     1321 agccgccgca ccgctcacga ggcggcggat gcggtcgcgc atctcgttgg ccgccttgtt
     1381 ggtgaaggtg acggcgagga tgccttcggg cgggacgtgc tcctcttcca tgagccaggc
     1441 gatgcggtgg gtgagcacgc gggtcttgcc agagccaggg ccggccacca caagaaccgg
     1501 tccctcgata gtggtgaccg cccgctgctg gtcgtcattc aggtcggcaa gaagagggtg
     1561 cgtctcggtc tgcatcatgg ctccatgtgg tgcgagggtt ctcgggcagg gtgggggcag
     1621 cggggtgccc ttgtcctcga gtgtagcgta ccggcagaag tcgcttcgac gcggtttgac
     1681 gacccatatc gcctttggta gcattcatat cggcacgttt gccgacgtag ctcagtggca
     1741 gagcaaagga ctcataagcc ttcggtcggt ggttcgaatc cacccgtcgg caccaatctg
     1801 gaccgccagc atgaatcgaa gattccacga cgatcagtcc cgggccccgg agtttctccg
     1861 tggcgaatcg ggactgattc agcgtgtgcg gcggttccta cgcgatcact ttcggggtga
     1921 ggcgccgcat ctggtcgtcg gctactcggg cgggcaggac tcgctcgcgt tgctgttggt
     1981 gctgggtgaa ctggagcggc tcggcgcttg ccgggtgact gctgcgcatg tcgatcatgg
     2041 attgcggccc gaggcggccc aagacgccga gcgagtcgtc gagatcggtt gcgctctcgg
     2101 tgtcgatgtg cgggtgtacc ggcctgaaac gccgctgcgg gagcagtacc gggggcggag
     2161 cgttgaggac gccgcgcggg tgttccggta ccgaacgctg gcgagcgttg tcagcacggt
     2221 caatgccgat gctatcgccg ttgcgcacca cgccagggat caagcggaga cggtgctgct
     2281 tcacgtgctg cgcggctcgg ggctcgccgg actctccggg atggcggcag acaaggtgct
     2341 gccggttcca ggctcgatgt ccggtgtcga gatgcgggtg attcggccgt ttctgcatga
     2401 atcgccggag acgctcgtcg agctggttga gcggtcgggc cttccgatca tcgaggaccc
     2461 aagcaatacc tctccggact tccggcgcaa tcgtatccgg cacgcactgc tgcctctaat
     2521 ggaggacatc gcgcccggtg ccaccggacg actcgtctcg ctggccgata tcctccgagc
     2581 cgacgaggag gcgctcgatg acgtcagcca cctcttgctg catcggacga cggacgatgc
     2641 cagtcgtctt gtctgggatc cgattcaggg ggcgccggtt gggttgcagc gtcgagtggt
     2701 gcgtcactgg atcctccaat ccggtttctc aggcgacttg agtcgggagc gcatcgaggc
     2761 ggtggttgag ctcaccaggc gcaacgaggg cggcaagcag atcgagatcg gcggtggctg
     2821 gtcggtgcgg tatgccgagg ggcgtctcga gcttcgtggt cggagtcatt gaaggacctg
     2881 tagtagcggg ggatctggct gtgctcggca atcatcgtcc acgtcaatgt tccgcgcggg
     2941 tcatgtcgtc ccgccgttgt ttcatcggta gttgtgcgag tccgggctga tcgagcgaat
     3001 gtccgcccgg cgtcgcggac cctcggtccg ttcctcgcgc agcgcgatga tccggatcac
     3061 cctcgggtcg acttcctcgc ggggctcctc gagcggatgt ccgctcctcg ggtcgtgacc
     3121 atcgtacggg tagaccaact gcatatcctc gatcggctga accgactccg ccacgacgtt
     3181 gatgttgttc ttcgcgtgtt gcagccgacc gtgaaccacc agcagcggcg ttgcgcggac
     3241 atggtgccgc tgtttctcgt agagccatgg gtggacgacg atgttgacca agtcgaactc
     3301 gtcctccagc aggagaaagg tgatgccctt ggcggtgccc ggtcgctgac ggcagacgat
     3361 cagtcctggc agccggatcg gggcgccgtc ggggagatcg cgcaaattcc gggtcgagac
     3421 catgtccttg ggcaggcgtg gccgcagcag cgccatcggg tggaaggtcg tcgacatgcc
     3481 gaggatgcgg tactcaccgg ccatgcgctc ccatgccgag gtgcgtggca gctcgacggc
     3541 atcctgctcg gtcggcagcg cgagcgagag ctgccggccc tctgtcttgt gctttcgccc
     3601 cttcgtgagt gcgcgtgagg ggatgaagag ccccagctgc cagatcatct cgcggcggtg
     3661 catgccgaag ccgtcgcagg cgccggcggc gatgaggtga cggaccgcct cctggcgcag
     3721 cggcacccgg cggatgagat cggccagcga gcggtagggt ccgtgcgcaa ggcgctccag
     3781 caggatcgcg gtggcgacat cctcgccgag acccttcacg aaggcgagtc cgatgcgcac
     3841 cgagcggcga cctgcgacgg tgcaggcaac ctcgctctcg ttgacatccg gccggagcac
     3901 gggaatgccg tgccgcttcg cgtcattgac gagcacatgc ggcgcgtaga agcccatcgg
     3961 ctggttgttg agcagcgcgc agaggaactc ggcggggtag tagtgcctca gccagcagga
     4021 ctgataggcg agcagcgcga aggcggctgc gtggctcttg gggaagccgt attcggcgaa
     4081 gccggtgagc ttgtcgaaga cggcctcggc cgtcttgtga tcgaccccct gaccgagcgc
     4141 tcccccgatg aacgtcgaac gcatcacctc catcgcctcg gcggagcgct tgcgggtcat
     4201 cgcgcggcgg agctggtcgg cctggccggg tgtgaacccg gcgagctcct ccgcgacctg
     4261 gacgacctgc tcctgataga ggatgacgcc caacgtctcc ttgagcagag gctccagccg
     4321 ggggtggtcg tactccggga tgtaatccgg gttctcgcgg cgctgtacgt agggcgtcac
     4381 cgcaccaccg acgatcggcc cgggccgaac gatggcgacc tgcaccacga ggtcgtcgag
     4441 tcgggccggc agcgtgcggc gcagcatctg gatttgcgcc cggctctcga tctggaagac
     4501 cccgaaggtg tcgcccgcct gaatcatgcg gtagacagcg gggtcgttga agtcgatgcg
     4561 actgagatcg accggattgg caccggattc gtcgatgagg tcgagcacct cctcgaccag
     4621 cgagagcata ccgagcgcga ggaagtcgat cttgatgaag cgcgcgtcct cgcaggagtc
     4681 cttgtcccac tggcacatga agcgtccctc catcgcggcg ggctggatcg gcacgatctc
     4741 gttgagcggc gtcgcggaga cgatcatgcc accgctgtgc tgagtgacgt ggcgaggaaa
     4801 gccggcgagc tgtttcgcaa gttcgatgag gtagctccac ggcggcgtgt tgcggcgtgc
     4861 ggcgtactcc ggcaggtggt tgagttcttc agctaggctg tcagccgagc gtggctcgct
     4921 cagccgagag atgcggtcaa ggtccgcgag gggcaacccg agtgccttgc cgacgtcacg
     4981 aacggcgctg cgcagtcggt aggtcgagaa ggcgcagacg agcgcggcgt gatcggcgcc
     5041 gtagacgtcg tagacgcgct cgatcaggcg ctcgcgaatc tcccggggga agtcgagatc
     5101 gatatccggg atcgagggca tctcctcatt gaggaagcgg ccgacgtaga gatcatgggc
     5161 gagggggtcg acgggtgaga ggccgattag gtagcagacg atcgagctga ccgatgagcc
     5221 gcgaccgcgt cccggcggca gaaaacggcg cgagccggag atgggcccgc cgcgcaactc
     5281 ggcggcaacc tcccgcgcga gctcgagcag gtcacggtag atgaggaaga atcccgcgag
     5341 gttgtgccgg gagataagtc gcagctcctc gcggatttgt tcccgcgccc tgtggttgtc
     5401 ctcactgtag cgctggggaa aggtctccca gcagatgcgg gcgagcgtct cgtctggcgt
     5461 ctcgccggtg ccggcgtcgt aggcggggaa ggtgtacttc aggtcccgtg taatgcggaa
     5521 cgcctcgcat cgctcggcaa tcacctgcgt gttcgcgatc gcttccggcc attcggcgaa
     5581 acggcgttcc atctccatgt gtgtcggcag cgcgaagcgg gcgttggggc gtcgctcgcg
     5641 atggcagcca tcaagggtgg tgtggttgcg gatagccacc agcacatcct gcaggcggtg
     5701 gcgtacggca gtgtggtaat ggacgttgcc ggtggcgaca cagccgagcc caatggcgtt
     5761 ggcgaggtcg acgagtgccc ggatgcggac ggtgtcgccg aagacctcgt tctgctgcag
     5821 ttcgacgaag acgttgtcgt gtccgaacca ggcgatgtac tggtcgagga tctgtatcgc
     5881 ggcacgcatg ttgttgtcgc caacaacgcg agagagctga cccttgcggc agccagtgag
     5941 caggatgatc ccctcggcgt agtcgcccag cggcgggttg cccggtgatg gctcagggtc
     6001 gtccgagagc gcgaccggtg ccgggtgacg gccgtggtgg agatgggtca gcactcggca
     6061 gaggttggcg tagccggtgg gggtctcggc gagcagggtg aggtgggagc catcggcgag
     6121 ggtgatctcc gcgccggtga tgggctggat gccggcttcc tcggcgagct gagcgaattc
     6181 gagcgcaccg tggagaccgt cgtggtcggt gatggcgagg gcggtgtagc cgaactcctg
     6241 ggcacgccgg accagctcgt cgggctggga ggcgccgtcg aggaaggaga atgacgtgtg
     6301 caagtggagc tcagtgtaca tgtcatcgtg gtcccgattg gagcgatctc cgcgtcaccc
     6361 tttcgtaggg gtggaccttg tgtccgctcg gccgcccgca aggcggcggc gcgataggct
     6421 gcaagcgacg caggctcggt aggcccgcgc ctcggtgggc gggctcgtgc ttggcggttc
     6481 tcgcgattgc ccgggcacag aggcccgggc ctaccgggat ggcgttccct ctggttcttg
     6541 cggctcgata gggacaacgc acgggggcag acttcacgct tgcccaatgg ggaacggtgc
     6601 accgcacgct agtagttctg ggtgaaccag gtgtcgttgg tgcgatcgtg aaagatggta
     6661 cggacgatcc caccctccag caccacctgg aaatactgtc ggctaatcgg atcgcgccac
     6721 cactcgtcgt cgatcatcca ggtgtcctgg atctgctcaa tccggtgacg gtgtgatctc
     6781 ccgaccagcg ttatgggccg gtctcgctcg accacgacct cgatctggcg ggggcggttc
     6841 aggggtcgaa gctgaccagc gcgtgacggc gttccggaat gcgagaccat ggctcgacct
     6901 ccacgatacg aaagagcggc gagagaccgt atcgctgctt cagcattgac acggcgtgcg
     6961 agagcggcgc gaggtggcgc gggcgcaggg cggggagcat cgcctggtgt gcgctcgtct
     7021 cgacgatgcc ctgaatctcg acggtgagcg actcgaccgg gcccggcatc tccagatgct
     7081 gcatgcggag cccgatcgcc tgcaccagcc agtccggccc atacgcgtcc ttgaagacca
     7141 tcgaccgctg ccacgatcgg ctgtgctcca gtccggcccg catggtgatc cctcgtgcgt
     7201 ggcggtagcg gagatcgggc gagcggaacg cccggtggac gagttgtttc agcgcgaaca
     7261 tcagcacgtc gtggctggtg gccggggccg gcagctcgag atactcggcg acggtaagct
     7321 cgcgggcagg cggctcgact ggatcgttcg cgtcgccggt gaccagatcc cgtgcgtgct
     7381 ggccggcggg accgaagcga gcgccgatcc gggcgcgcgg gatggcgacg aattgcccga
     7441 gtgtctcgat gcccatcagg tgcagttcgt ggatcgtgcc ggcgtcaagc ggcagcagct
     7501 cgaccggcgc gcccgccagc atcgcctgtg ttcgcgcagg tgggacgagc gagacggtgc
     7561 cgggggtgct gcggttggcg gcgacccgcg ccgcgaactt gccgacggca actccggcgc
     7621 gcggccgcag accggaatcc acctgccgta gcaggtgctc ccccgcctca agagtcgtgt
     7681 catagtggcg gccgagtccg gtgagatcga tataccaggt gccggggtcg ccggcgtccg
     7741 gctggacgag cgggctgata gtgtcgagtg cggcagtgat ggcgtgcagc tgctcgtgct
     7801 cagtggcggg attcgacgcg atgatgatgg cgttcgggac gagcgcggag gcctccctga
     7861 gagagaggcc ggtgcggatg cctttctggc gtgcctcggg cgtggcatcg agcacggacg
     7921 ggtggccatg gtgcggatcg ctgaggatga gcggatggcc gtccagttcg ggttgggcga
     7981 gcaaggccac gcgcagcccg aagtggggaa cgtggagaca ggcgatggac atcgatggga
     8041 cgcctcctga tcgatcgaaa tggcaggatt gccgggtcgt ctgagccggc aattctgccg
     8101 tttcgagtca tcctttggac atgaccgccg tggtgagaga tgaggggtgg tcgaagccgt
     8161 caacagcgag aacgatgagg ctgatgcacg attccatgtg gcaccctttc acggcaacgc
     8221 ggatcacaat cgattctagc ctgagattag aacatatgtt ctaataccgc aagatgtgtc
     8281 ctgcagtcac cgaaatgcct ggtgggtcgc aaaggggcgc ggtcgagtgg gtcgtagtgg
     8341 cgattcctcg taacctgtgg gcctacgttc ctgggtcgaa accgtaacga gcgtcgtcac
     8401 taccgattga atcgaacccg acgctgccat cagtcacctc ccggccggca tcgtggcctc
     8461 atttctgcca atgtggaagg ggccgaaatg cacggatctt cggtacacta cacggcagca
     8521 tgagacaatc gatcacaccg ccaacgccag cgacaggccg catggcgcag cgagagggag
     8581 ccaatgtgag tgaagggaat caggcgatgg cggtgccagc cgaggcgcgg gccgaggggg
     8641 tcgcccgggt gctgatctcc gagcatgact tgcaacagga gatccagctg atcgcgcatg
     8701 agctcgacga ggcatacggc gacgaccgac cgctcatgat cggtgtgctc aaaggcgcgg
     8761 tcgcgttcat ggccgatgtg ctcaaggcaa tgaccatccc cgtcgagatc gatttcatgg
     8821 ctgtctcatc ctatggtgcc tcgacccagt cgtccggcgt ggtgcggatc ctcaaggatc
     8881 tcgatcagga aatcgaggga cgccgggtca tcatcattga ggatatcgtc gacagcggtt
     8941 tgacgctgca gtacctgctc gatctgctgg agcggcgcag tcccaaggac atccgggtcg
     9001 tcgccctact gaaaaagatc aagtcagatg ctgcccaggt gcaggtcgac cgggtcgcat
     9061 tcgagatacc ggatgagttt gtcgttgggt acgggctcga ctacgctgag gggtatcgca
     9121 acctcccgta cgtcgccgtg ctcgatccgt cggtctactc ggaaccggac ggagggtagg
     9181 ggattggtat acttcggaca agtgttgacg taaggccccc cagggcgtat tcaacacaca
     9241 ttctggctca catcaccacc tcaacggaga catcacatgc cacgacggaa atggatgcgc
     9301 agcggtgcca tctggatcgt cctgctggtc ggcac
//
LOCUS       DASYWL010000168         3446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_645876, whole genome shotgun sequence.
ACCESSION   DASYWL010000168 DASYWL010000000
VERSION     DASYWL010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645876"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VGR22_06030"
     CDS             <1..383
                     /locus_tag="VGR22_06030"
                     /inference="COORDINATES: protein motif:HMM:NF044669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128159.1"
                     /translation="PVAAEVADLRARWAEVVERASPVIKPLLKECRPVARDGARLTLA
                     FPEGRDFMRSRIAQRSAAIESLLSEMFGGQFAIECVASNLELEPLTVAQAIGDNPEDP
                     EAQALLEGVLKITGGELVDAPEVR"
     gene            425..751
                     /locus_tag="VGR22_06035"
     CDS             425..751
                     /locus_tag="VGR22_06035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEV2128160.1"
                     /translation="MNIGQIAKMAQQMQAQMAQAQEELKETTLEATAGGGAVKVVITG
                     AQEVRSIEIDASAVDPDEVEMLQDLVLSAVNEAIGRSKELERERMGSIAGGMGMPGMP
                     GLPGFG"
     gene            759..1370
                     /gene="recR"
                     /locus_tag="VGR22_06040"
     CDS             759..1370
                     /gene="recR"
                     /locus_tag="VGR22_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEV2128161.1"
                     /translation="MAGLIAPVARLIEEFSKLPGIGTKTAQRLAYHVLRTSPDDARAL
                     AAALIAVKEDVAYCSVCCNITEVGNDPCDICADHRRDTTRICVVEEPLDVLAIERTGE
                     FRGRYHVLHGAISPIDGIGPDRLRTRELMARVGKGGVEEVILATNPNLEGEATAMYLA
                     DVLAPHVASVTRIARGLPVGGDLEYADDVTLIRSLQGRRLVER"
     gene            1736..3240
                     /locus_tag="VGR22_06045"
     rRNA            1736..3240
                     /locus_tag="VGR22_06045"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          691 a         1029 c         1156 g          570 t
ORIGIN      
        1 cgccggtggc ggccgaggtt gccgacctgc gcgcccgctg ggcggaggtc gtcgagcgcg
       61 ccagcccggt gatcaagccg ctgctcaagg agtgtcgccc ggtcgcccgc gacggcgccc
      121 gcctgacgct cgccttcccg gaggggcgcg acttcatgcg cagccgcatc gcgcagcgtt
      181 cggcggcgat cgagtccctg ctgtccgaaa tgttcggcgg ccaattcgcc atcgagtgcg
      241 tcgcgagcaa cctcgagctc gagccgctga ccgtggcgca ggcgatcggc gacaatcctg
      301 aagaccccga ggcgcaggcc ctgctcgagg gcgtgctgaa gatcaccggc ggcgagctgg
      361 tcgacgcacc agaggtgcgc tgaccgccgc gccatccacc atcggtacag aggaccccac
      421 acccgtgaac atcggtcaga tcgccaagat ggctcagcag atgcaggcgc agatggcgca
      481 ggcccaggag gagctcaaag agacgaccct ggaggcgacc gccggcggcg gcgccgtgaa
      541 agtcgtgatc accggcgccc aggaggtccg cagcatcgag atcgatgctt ccgccgtgga
      601 ccccgacgag gtcgagatgc tccaggacct ggtgctcagc gccgtcaacg aggcgatcgg
      661 gcgcagcaag gagctcgagc gcgagcgcat gggctcgatc gccggcggga tgggcatgcc
      721 cggcatgccg ggcctccccg gcttcggttg agcgcgagat ggccgggctg atcgccccgg
      781 tcgcccgcct catcgaggag ttcagcaagc tccccgggat cgggacgaag accgcccagc
      841 gcctcgccta ccacgtcttg cgcacgtcgc cagacgacgc tcgcgcgctc gccgcggcgc
      901 tgatcgccgt gaaggaggac gtcgcctact gcagcgtgtg ctgcaacatc accgaggtcg
      961 gcaacgaccc gtgtgacatc tgcgccgatc accgccgcga cacgacgcgg atctgcgtcg
     1021 tggaggagcc gctcgacgtg ctcgccatcg aacgcaccgg cgagttccgt ggccgctatc
     1081 acgtcctgca tggcgcgatc agcccgatcg acggcatcgg cccggaccgg ctgcgcacgc
     1141 gcgagctcat ggcacgcgtg gggaagggcg gcgtggagga ggtcatcctc gcgacgaacc
     1201 cgaacctgga gggggaggcg accgcgatgt acctcgccga cgtgctcgct ccgcacgtgg
     1261 cgtccgtcac ccgcatcgcc cgtggcctgc ccgttggtgg cgacctggag tacgccgacg
     1321 acgtgaccct gatccgttcg ctccagggcc gccgtctcgt cgagcgctga acgtgccagg
     1381 gtccttgacg gcatcgaaca cctgttctag aatgaccttc gaacggacga gcggatcggc
     1441 tccgacgcgg gcccgcttga cggtcccgag aggctgatct atagtccgtc ttcgcgccgg
     1501 cagctcgccg gccgatgtgc cgcgaacggc gaccgaccag gtcgtgcagc gtgaccatcg
     1561 cgaggatccg taacaggagt actcgaatcg aacgaggcca ggcggtcggc accgagccga
     1621 tcaggttggc ggcttaccag ctgaagagtg acaggaatcc acacgagtca gcgggacaac
     1681 gtcgaactct taaccggtgg agcagacctt cgggtccaga ccactggttt cgttttgtac
     1741 ggagagtttg atcctggctc aggacaaacg ctggcggggt gcgtgagaca tgcaagtcga
     1801 acgaggtgcc atcgagcttg cttgatggct accgagtggc ggacggctga gtaacgcgta
     1861 ggtgacctgc ccggaagtgg ggaataactg cccgaaaggg tggctaatac cgcatgtggt
     1921 cggcgattca tttcgccgtc taaaggagaa attcgcttcc ggaggggcct gcgtccgatt
     1981 agctagttgg tggggtaacg gctcaccaag gcgacgatcg gtagctggtc tgagaggatg
     2041 atcagccaga ctgggactga gaacggccca gactcctacg ggaggcagca gtcgggaatt
     2101 ttgcccaatg gacgaaagtc tgaggcagca actccgcgtg agggatgaag gctctagggt
     2161 cgtaaacctc ttttctcagg gaagatacaa gacggtacct gaggaataag ccacggctaa
     2221 ctacgtgcca gcagccgcgg taatacgtag gtggcaagcg ttgtccggat ttactgggcg
     2281 taaagagcgc gcaggcggct ggtcaagtcc gatgtgaaat ctcccggctc aactgggaaa
     2341 ctgcatcgga aactgatcgg cttgaaggtg ggagagggca gtggaattcc cggtgtagtg
     2401 gtgaaatgcg tagatatcgg gaggaacacc agtggcgaag gcgactgcct ggcccattct
     2461 tgacgctgag gcgcgaaagc taggggagca aacgggatta gataccccgg tagtcctagc
     2521 cgtaaacgat gaacactagg cgtagtcggt atcgaccccg gctgtgccgc agctaacgca
     2581 ttaagtgttc cgcctgggga gtacggccgc aaggctaaaa ctcaaaggaa ttgacggggg
     2641 cccgcacaag cagcggagcg tgtggtttaa ttcgtcgcaa cgcgaagaac cttaccaggg
     2701 cttgacatgt accggaaaac ggcggaaacg tcgtcctccc gcaagggacc ggtatgcagg
     2761 tgttgcatgg ctgtcgtcag ctcgtgtcgt gagatgttgg gttaagtccc gcaacgagcg
     2821 caacccccgt ggcatgttgt atctctcatg ccagactgcc gagagaaact cggaggaagg
     2881 tggggatgac gccaagtcag catggccctt acgccctggg ctacacacac gctacaatgg
     2941 ccggtacaac gggttgccaa accgcgaggt ggagctaatc ccttaaagcc ggtctcagtt
     3001 cggattgcag gctgcaactc gcctgcatga agccggagtt gctagtaacc gcaggtcagc
     3061 acactgcggt gaatatgttc ccgggccttg tacacaccgc ccgtcacgtc atgaaagccg
     3121 ataacacccg aagccggtgg gccaaccgca agggggcagc cgtcgaaggt gggattggtg
     3181 attgggacga agtcgtaaca aggtagccgt accggaaggt gcggctggat cacctccttt
     3241 ctagggagat atgtggaacc ggttcgccgg atccaccgat cccaggtcga tcggccagct
     3301 cgccgaaagg atcgatgctc gagggagcat cggtgaacga gctggacgcc ctcggaatga
     3361 cctgattccc gctgataacg cggattcctg tcactcttca gcctgacaag cgcgctgaag
     3421 ccgcctcgag agcggcggct ttttcg
//
LOCUS       DASYWL010000169         1820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_402301, whole genome shotgun sequence.
ACCESSION   DASYWL010000169 DASYWL010000000
VERSION     DASYWL010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402301"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            680..907
                     /locus_tag="VGR22_06050"
     CDS             680..907
                     /locus_tag="VGR22_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128162.1"
                     /translation="MPRRQRGHRTVVTDVADTARSRRTANRADRHLSVGTVCSVRQQL
                     SAYCDEAKPYDERDDTLKPNAPGVIGLRGDC"
     gene            complement(1097..1537)
                     /locus_tag="VGR22_06055"
     CDS             complement(1097..1537)
                     /locus_tag="VGR22_06055"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV2128163.1"
                     /translation="MLTLLASLEGVGDGGAISELDHALQLASRAERAGADEDVVLAGL
                     LHDIGKVFGDAGHGAIAAALLEPHVRPDVVQIVRHHSQFTARHWEQIPPGEPDPRDQF
                     AEEPWFQLACRFVDEWDMKSFDPAYDSLPLDHFIPLVQRRVREP"
BASE COUNT          319 a          600 c          591 g          310 t
ORIGIN      
        1 aggtaggggt aggggggaag atctccctct acaatagcgc cggctcggta catgtcgtcg
       61 ccgatgtagc tgggtggttc ggcgcctgac ctgagatgcg cctgcgacca ccagccggcg
      121 acggttgctc tcctggagcc gagcgacctc gacctggtgt tgtgtgacct cgactggcct
      181 ctgccctatg tatgaagccc ctgatcagcg cgtacacgct cccgcaatgc tcgcaggtcc
      241 gctcccggac atcgcggtca ggatcaagtc gcagctcggc atctccccgg aatccgggag
      301 gcttccgtct gcgtcgcagc atgcgagcag tttggctttg caggactccc gggccaccac
      361 gagcgcgtgc aggagaactc acctagacgc agggtccgac ttgtgcggtc agggcacccg
      421 agcaggaaca cgagggcgct ggccttacct tgtcctgtcg gtaatcatct tcgggctgca
      481 gcacgggaag gtgatgttca gcgacgacta ctccttcaag cggacgtcgg tggaggtcgc
      541 tgtcatcgcc ttggttgtcg gtgccattgt cgtgctcgtg gagttgctct ggtaaacgcc
      601 atccgacaac cgcggaccgg cgaagggcgg tggaaagctc gcggtgtgcg agcgaacacg
      661 ccgggatgga ccagccgcca tgcctcggcg ccagcgtggc caccgaacag tagtgaccga
      721 cgttgccgat accgctcggt cgaggcggac ggcgaaccgg gcagatcggc acctatcggt
      781 cgggaccgtc tgctccgtgc ggcagcagct ttccgcctac tgcgatgagg ccaagcccta
      841 cgacgagcgc gatgacaccc tcaagcccaa tgctccgggt gtcatcggcc ttcgcggcga
      901 ctgctaaagc aattgtggcg atgcagcaag ccactcccgc caccaaagct agccatccga
      961 gggccgggcg catcccagca gtatcaccgg gttccgccga tcccgcccgg gttgctcgat
     1021 gccgccaccg atgcgaaccg gcacctatcg cacgcagggc cagcgtggcc tgcggaagcg
     1081 ctgccggcgc gctcgactat ggctcccgta ctcgccgctg gaccagggga atgaaatggt
     1141 ccagtgggag agagtcgtac gccgggtcga acgacttcat gtcccactcg tcaacaaacc
     1201 ggcacgccag ctggaaccaa ggctcttcgg cgaactgatc cctcgggtcc ggctctccag
     1261 gcgggatctg ctcccagtgt cgagcggtga actgggagtg gtgacggacg atctgaacga
     1321 catctggccg aacatgaggt tcgagcagcg cagccgcgat cgctccgtgg ccggcgtcac
     1381 cgaacacctt gccgatgtcg tgcagcagcc cggcgagcac gacgtcctcg tcggcgccgg
     1441 cgcgttccgc ccggctggcc agctggaggg cgtggtcgag ctcgctgatg gctccgccgt
     1501 cacccacgcc ctcaaggctg gcgaggagtg tcagcacctg ttcggccctc cgtccggggc
     1561 gacagagccg gtccataccc gcatgctcgc ataaggacgc caatgtccga cgtcattttc
     1621 gcccgaggtg acagggacgc tagctctccg actcgccacg gcgagcggtc ggtaagacca
     1681 tgcctcgctg ataccgcccg acatcgcgcc ctgccgggcc gatgatcaac ggcacgtccc
     1741 ggccggggtg tcctcagcga actcgatcga ccgccgagac gagcgttgag gaggacagct
     1801 gttttcttaa catagcgagg
//
LOCUS       DASYWL010000170         9721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_195849, whole genome shotgun sequence.
ACCESSION   DASYWL010000170 DASYWL010000000
VERSION     DASYWL010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9721
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195849"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(299..490)
                     /locus_tag="VGR22_06060"
     CDS             complement(299..490)
                     /locus_tag="VGR22_06060"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2128164.1"
                     /translation="MVRLGRVTVLVRDVDESKGFYADAFGFETLFDEEIDGGFRAVHV
                     GSEGIRGAGLWLMPATGTP"
     gene            749..1486
                     /locus_tag="VGR22_06065"
                     /pseudo
     CDS             749..1486
                     /locus_tag="VGR22_06065"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            complement(1499..2371)
                     /locus_tag="VGR22_06070"
     CDS             complement(1499..2371)
                     /locus_tag="VGR22_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128165.1"
                     /translation="MAEVRKVPVPAAYRLDVSAAPAAWVGERSSRHAWVDGSLVWCGR
                     EGDRAVWRRVRQPGAGLLTIEGSASSALDGRWVAATLNPDCMVARWNDPVLSRIAERY
                     PGLAPYGDGSLFEGLVTSIIGQSISVASATVTQARLARLFGGAIEVAGRHFAPLPNPS
                     QLADASVALIHTSGVTSRRAEALKRIAAFAVTGELPSDERARSEPEGVQRELLALPQV
                     GPWTASSALLWGVGAPDAWPTGDVAVLRATRYALGDDSVTLKTMDTVAERWRPHRGIA
                     ARLLWTNLFGVGRL"
     gene            complement(2364..3476)
                     /locus_tag="VGR22_06075"
     CDS             complement(2364..3476)
                     /locus_tag="VGR22_06075"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV2128166.1"
                     /translation="MTTAAIPTEEARQVTPLANDLGARFAVEFDDSGEEMLNDGTHRY
                     RRVAFALFFVYMAVAVGIYLWRGVWFTPDRWAVFLLIGALLLGRVGSFLRDWIPFVLL
                     IFGYEYLRGIAGEIVVGGQPVWELPRNSMPAVRLEGLIRFDEVLFFGHSPILLIQQQI
                     YDPGNPRWWDFLALVVYSMHFVLPCVFAFALWLTSKRRFWTFTLAFCFMTYTAFAFFL
                     LYPAAPPWLAQAWDVASGIGFPQNHVFNVIQPHGFETLDTYAIWGNASPHPVAAMPSL
                     HAAFPWLVMLFAVRYYGWKGLWFLLYNVALWYAVLYTANHWFVDVLAGIGWASVAFFV
                     VVGALAMFEQHPKWRARLPWAPIGRRRAVTEATLGG"
     gene            3571..4338
                     /locus_tag="VGR22_06080"
     CDS             3571..4338
                     /locus_tag="VGR22_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128167.1"
                     /translation="MPNLPTTVLVAWAPSIVGVAAPLTEVLPPTVDTLSVVPAPLERG
                     ISSIGETRSAWQRHGIRTSMLLPHPPVWSVLSPGASRWHRIDAAGLALRLDARIVARP
                     YAAVLALTDNLRQGPFVLDLPARFLHPVDRLRLAARPGGLRLQADVAAVALPAASILL
                     TPVADGWLALSGTDPIATELWALSLAERWLGRDIEMQGPWEDPSVQRATELELGVRIP
                     SDMTIRAASDAPLPSVARELLEETASRLGMQVPDTIG"
     gene            4773..6224
                     /locus_tag="VGR22_06085"
     CDS             4773..6224
                     /locus_tag="VGR22_06085"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEV2128168.1"
                     /translation="MVETVRDGSPVVLGVPRERADAERRVALVPEVVRRLIAGGASVV
                     VETGAGDGSSVPDHLYEEAGAEIVDQPEDVYERADMVLKVQAPARWDDFDEIAALRGG
                     QYLLAFLSPLTKHDLINELRDKGVEAFSLDAIPRTTRAQAMDALSSQSNLAGYKAVIM
                     GADALGRIFPLMMTAAGTITPARVLILGAGVAGLQAIGTAKRLGAVVYGYDVRAVVRE
                     QVESLGARFVQLDLGEDLAGAGGYARQATEDQTRRQQQELAKEIALADVVITTALIPG
                     KPAPVLITAEAVAGMKPGSVIVDLAGEMGGNCELSQFGETVVEQGVTIMAPVNVPSLV
                     ANHASQVLAKNIQNFLDLILRNGQLDVDFGDDIVAGTAVVHDGEIVHALTRSAMGLPE
                     LAGAAPEPAPAAEPQTVEEMPEPERVDEPEGTEEAVIHEPGTLEPGETAVITPESEPT
                     TTPDEVFDDIDGEIDDEPEDDDRRPRSEPGENA"
     gene            6221..6541
                     /locus_tag="VGR22_06090"
     CDS             6221..6541
                     /locus_tag="VGR22_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEV2128169.1"
                     /translation="MNEEFLVGTYVFLLAVFLGVELIGRVPSTLHTPLMSATNAIHGI
                     VMIGGMILLGDVDNIALTILGFVAVAFGAANIVGGFVVTDRMLGMFRRRPGAASGGEA
                     GSQR"
     gene            6538..7950
                     /locus_tag="VGR22_06095"
     CDS             6538..7950
                     /locus_tag="VGR22_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEV2128170.1"
                     /translation="MIENGREAVIDVLYLASAILFILSLRMLSAPRSARNGNLLAMVG
                     MGLAVLATFFLEGVFDKIIWILVAFAIGGGGGYLAAKRVKMTEMPQMVAIYNGLGGGT
                     VALVSVGEFLSREDLGRATELTIILGTIIGAISLMGSIVAFAKLQGLISGRPIKLPNQ
                     QLYNGAAFVAIVVLAVLVLVLGEGALDKSLLIVMFLITLAIGVALVLPIGGADMPVVI
                     ALLNAATGLAAAITGFTMDNQALIIAGSLVGASGTFLSILMARAMNRSLVNIVFGAFG
                     AGPAAGAGPGAVEAGEALPIKSTTAEDAAVTLAYANSVIIVPGYGLAVAQAQHATREL
                     ADVMRERGVEVRYAIHPVAGRMPGHMNVLLAEANVPYEDLYEMDRINDDFRNTDVVLV
                     IGANDVVNPAANNDPNSPIYGMPVLHANEARNIIVLKRSMDPGFAGIENMLFHDPKTS
                     MLFGDAKDSLNELVAAVKNV"
     gene            8025..8453
                     /locus_tag="VGR22_06100"
     CDS             8025..8453
                     /locus_tag="VGR22_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128171.1"
                     /translation="MANDPDTRFDDIMNRLEQAWGLWQDAIGRVSESDARDVALARSL
                     MHETAVGDEQAVEVIRSLVEGSTYQQLPGRETVEEADDLDGVRSIMEHHHERLLGSLD
                     TVAETAEDIVVAVEEQVGPLTWEQYPVRAERLTTTGDASA"
     gene            8457..8897
                     /locus_tag="VGR22_06105"
     CDS             8457..8897
                     /locus_tag="VGR22_06105"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEV2128172.1"
                     /translation="MGRLTLLASLLMPAMLLAGCVALPSLNAQPAVCVPPARHPSLAK
                     VDGLYVLPDDGRAPVLDEIDRASCTIDLTIYLLSDDATIAALVRAEARGVKVRILYEP
                     APFGGGVGLVETTEDLVRHGVELRSGSEGVALIHAKYLVTDRSP"
     gene            8951..9202
                     /locus_tag="VGR22_06110"
     CDS             8951..9202
                     /locus_tag="VGR22_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128173.1"
                     /translation="MITTVPADVVTLASIFEADWSRFTPTVSTDRMVLSPGNGRAALL
                     EQILGAESSIRLYAEVIRDDEIIDALSTVAKRGVSYSSW"
     gene            9199..9486
                     /locus_tag="VGR22_06115"
     CDS             9199..9486
                     /locus_tag="VGR22_06115"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEV2128174.1"
                     /translation="MNEPEDDLDETVYDATLNSNGVEVRHTGSLYIHAKGLIVDEQAV
                     VVGSHNPTAQSLDRNREVSLVIEGAPAVKRGVDVFDRDWLQALPDQFRKGG"
BASE COUNT         1782 a         3140 c         3086 g         1713 t
ORIGIN      
        1 gtgtacaaca ggcgcgcgac gctcagcgtc gggcttgttc aagtatatga tgcggtcagt
       61 ggatgtccgg cacggatggg atcgtccttg ctcggacctg gtcgcctctt ttgcgctcaa
      121 tcattgttat acgcaaagga cggtcgcaga caggttgtcc ttctggccgc tatcccctcg
      181 cggtgtcctg ggtcatgtat gcgctcaatg ggccaactgg acgagcacga cctcgttact
      241 cgaattgtcc cgtacgtgcg cgaactccga tccggcagcg tcggactgcg gcacgacgtc
      301 aaggcgtgcc agtcgctggc atgagccaca gccccgcacc gcggatgccc tccgaaccga
      361 cgtgaacggc acggaatccg ccgtcgatct cctcgtcgaa gagcgtttcg aatccaaagg
      421 cgtccgcata gaaccctttt gattcgtcga cgtcacgtac cagaaccgtc actcgcccaa
      481 gccgcaccat ctgcttcctc gttgcttaac gcacgaattc gttgtcattc ttcggcagag
      541 attgtagcaa cggcgcacat cgcaaccggg gaagacgaac gcagctggtc cgacaaagta
      601 gcgcgtatca tcataatgat acggcgaccg gatcagtgtc cgagcatgaa acagcgagcg
      661 tgcaatggat gcatcacacc tcactcccct cttgcgcatt gggctggcgg ccgtgcgcaa
      721 cgcctcgtga gtcgatgagc gccttgccac gctcgatcgg tttctggccg acgcggcgtc
      781 caaggatgtc gcgatcatct gcttcccgga ggcctacatt cccgggctgc gcggcttcga
      841 ctttcccgtc ccgccgcagg atcagaagcg ccagcaggtc gccctcgata ccatctgtgc
      901 ctccgcaggc gccacaacgt cgccgtcgtc gtcggcatgg gaggcggcgc tgggactcca
      961 caatgtggct ttcgtcgtct cccgcagcgg cgatcccctc ggctaccaag ccaagaacca
     1021 gatcccgccg gaggaagcac cgtattacgt gccagaccgg aagcggaggc tgttcgagat
     1081 cgacggcgtc cccttcggca tcacgatctg tcacgagggc tggcgctatc cggaggccgt
     1141 tcgctggtcg gcggttcgtg gtgcgaaggt ggtgtttcac ccgcaactga ccggtagtga
     1201 ccgcaacgac ccgaccatcg agcgctgggg cgaccccgga tcgccatact acgaaaaggc
     1261 catgatcatg cgagctgtcg agaacagcgt ctggttcgcg agcgtcaact acgcgatgcg
     1321 ctaccaggag cccgctacga cggtgatcgc gccggacggc tcctgcgagg ccttcgtgcc
     1381 gtacagcgag gagcgcctgc tggtctgtga tcttggcctt tcccgcgcga ccggcttcta
     1441 cgcaagacgg tttgacccgt cgctttatcc caatgcagct gaatgatgct tgggattgtt
     1501 acagccgccc gacaccgaac agattcgtcc acaggagtcg tgccgcgatt ccacggtgcg
     1561 ggcgccaccg ctcggcgacc gtatccatcg ttttgagcgt gaccgaatcg tcgcccaagg
     1621 catatctggt cgccctgagc accgccacgt cccccgtcgg ccaggcatcc ggcgcgccca
     1681 cgccccacag cagcgcggag gacgccgtcc acgggccgac ctgcggcagc gcgagaagct
     1741 cacgctgaac gccttccggt tccgaccgcg cacgttcgtc actcggcaac tcacccgtga
     1801 ccgcgaatgc tgcgattcgc ttgagcgcct cggcgcgacg tgaggtgaca ccggaggtgt
     1861 ggatcaacgc cacggatgcg tcggccagct gcgacggatt gggcaacggc gcgaaatgcc
     1921 gaccggctac ctcgatcgca ccaccgaaga gtcgagcgag ccgcgcctgt gtcactgtcg
     1981 ccgatgcaac ggaaatgctc tggccgatga tcgacgtgac cagcccctca aagagcgaac
     2041 catcgccgta cggcgcgagt cccggatagc gctcggcaat acggctcagc accggatcgt
     2101 tccagcgggc gaccatgcag tccgggttga gggtagcggc cacccaccgc ccgtcgagag
     2161 cggaggacgc actcccctcg atcgtcagta agcctgctcc cggctgccga acccgtcgcc
     2221 agactgcccg atccccttcc ctgccgcacc agacgagcga gccgtcgacc caggcgtgtc
     2281 gtgaggagcg ttcccctacc cacgctgctg gcgcggcgct gacatccagc cgatatgccg
     2341 ccgggacagg cacttttcgc acctcagcca cccaatgtcg cctccgtcac cgcgcgtcgc
     2401 ctgccgatcg gtgcccaggg caaccgcgct cgccatttcg ggtgctgctc aaacatggcc
     2461 aacgccccta cgacgacgaa gaacgcgacg cttgcccagc caatcccggc caggacgtcc
     2521 acaaaccagt ggttggcggt gtacagcacc gcgtaccaga gtgctacgtt gtagagcagg
     2581 aaccagaggc ccttccaacc gtagtagcgc acagcgaaaa gcatcaccag ccaaggaaag
     2641 gcggcgtgca acgacggcat cgccgcgacg ggatgcggac tcgcgttgcc ccagatcgca
     2701 taggtatcga gtgtctcgaa cccgtgcggc tggatgacgt tgaagacgtg attctgcggg
     2761 aagcctattc cgctcgcgac gtcccacgcc tgcgccagcc agggcggtgc ggccggatag
     2821 agcaggaaaa aggcgaaggc ggtgtaggtc atgaagcaga acgccagcgt gaaggtccag
     2881 aatcggcgtt tgctcgtcaa ccagagcgcg aacgcgaaga cgcagggcag cacgaagtgc
     2941 atcgagtaca ccacgagcgc caggaaatcc caccagcgcg ggttgccggg gtcgtagatc
     3001 tgctgctgga tgaggaggat gggtgagtgt ccgaagaaga gcacttcgtc gaagcggatc
     3061 agcccttcga gccgaaccgc cggcatcgag ttccgaggca gctcccagac cggttgcccg
     3121 ccgaccacga tttctcccgc gatcccgcga aggtactcgt agccgaagat gagcagcacg
     3181 aagggaatcc agtcgcgcag aaagctgcca acgcggccga gcagcagcgc accgatcaac
     3241 aggaaaactg cccagcggtc cggcgtgaac cagacgccgc gccaaaggta gatgccgacc
     3301 gcgacggcca tatagacgaa gaaaagcgcg aaggcgactc ggcggtaccg gtgggtgccg
     3361 tcgttcagca tttcctcgcc cgagtcgtcg aactcgaccg cgaaacgggc accgagatca
     3421 ttggcaagcg gcgtgacttg tctcgcctct tctgtcggaa tggcagccgt ggtcacggga
     3481 gtctcccggt agcagcgcgc taatactcga cttcgcggta ttgtacgtga atgcatcgca
     3541 ttgccctgtc gaccaattcc cgggaacgtt gtgccgaacc tgcctaccac cgtccttgtc
     3601 gcctgggcgc cgagcattgt tggcgtcgcg gcaccgctca ccgaggtgct gccacccacg
     3661 gtcgatacgc tgtcggtcgt ccccgctccg ctggagcgcg ggattagttc gatcggagag
     3721 acccgctccg cctggcaacg ccatggaatt cggacctcaa tgctgctccc tcacccgccg
     3781 gtatggtcgg tgctctcccc tggcgcttcg cgatggcacc gcattgacgc ggctggcctg
     3841 gcgctgcgcc tcgacgcgcg aatcgtcgcg cgtccatacg cagcggtcct ggcgctcacc
     3901 gacaacttgc gccaaggtcc atttgtgctc gatcttccgg cgcgcttcct gcacccggtc
     3961 gatcggcttc gccttgcggc gcgaccgggc ggtctgcggc ttcaggcaga tgtggctgcc
     4021 gtggcattac cagccgcttc gatcctgctc acgccagtcg ctgacggttg gctcgcgctt
     4081 tccggcacgg accccatcgc cactgagctg tgggcgctct ccctagctga gcgatggctc
     4141 ggccgcgaca tcgagatgca gggtccctgg gaggacccct cggtgcagcg tgccacggag
     4201 ctggaattgg gcgtccgaat cccgagcgac atgacgattc gggcggcgag tgacgctccg
     4261 ctcccctcgg tggcacgcga gttgcttgag gagaccgcaa gtcgtttggg tatgcaagtt
     4321 cccgacacaa tagggtagca ggcgaaccga aacggccctt gtcacacagc ggtgctccgc
     4381 tatactcgct tctacacggg tggaggaact aagcaccacg gaggccggtg tcggtgcata
     4441 caccggtcgc agggaagggt atccgcacca tggaaacatt gcgatcgatc tgggatgcca
     4501 tcgtccgtcg gctcggtgta cagcagccgg agctcgtgcc cgtaccagtt acccgaccgc
     4561 aacgcgtccg acgctagacc cacaaaatac caaacacaat cccaagaacg agccgtccgg
     4621 accggacggc tcgttctgtt ttcccgactc ggatgcccga cttccacttg cggaagcgaa
     4681 ccaagatagt acactgccga aaacctcgac atccggcgca cgccgcaggc gtcgcgccag
     4741 aaatggctgc agggtacagg aggagcattt ggatggtgga aactgttcgg gacggcagcc
     4801 cggttgtgct cggggttccg cgggaacgtg cggacgctga acggcgcgtc gccctcgtgc
     4861 cggaggtcgt ccggcgcctg atagctggcg gcgcgtcggt ggtggtcgag actggtgcag
     4921 gagatggatc gtcggtacct gaccacctct acgaggaggc aggagccgag atcgtcgacc
     4981 agcccgagga tgtctacgag cgggccgaca tggtgctcaa ggtccaggcg ccggcacggt
     5041 gggacgactt cgacgagatc gccgcgctgc gcggggggca gtacctgctg gcgtttctct
     5101 ccccgttgac gaagcacgat ctgatcaacg agctgcgcga caagggggtc gaggccttca
     5161 gtctcgacgc catcccacgc acgacgcgcg cgcaggcgat ggacgccctg tcctcccaga
     5221 gcaacctggc cggctacaag gcggtgatca tgggtgccga tgctctcggc cggatcttcc
     5281 cgctgatgat gaccgccgcc ggtacgatta cgccggcccg ggtgctcatc ctcggtgccg
     5341 gcgttgccgg actccaggcc atcggcacgg ccaaacgcct cggcgcggtc gtctacggct
     5401 atgacgtgcg cgcagtcgta agggagcagg tcgagagcct tggcgcgcga ttcgtccagc
     5461 tcgatctcgg cgaggacctc gccggagcag gtggctatgc ccggcaggcg accgaagatc
     5521 aaacccgccg gcagcaacag gagctcgcca aggagattgc cttggccgac gtcgtcatca
     5581 ccacggccct catccccggc aagcctgcac cggtgctcat cactgcggaa gcggtggccg
     5641 gaatgaagcc cgggtcggtg atcgtcgacc tggccggcga gatgggcggc aactgcgagc
     5701 tctcccagtt tggcgagacg gtggtcgagc agggtgtgac gatcatggcg ccggtcaacg
     5761 ttcccagcct cgtggcgaat cacgcctcgc aggtgctcgc caagaacatc cagaacttcc
     5821 tcgaccttat cctccgaaac ggccagctcg acgtggactt cggcgacgat atcgtcgccg
     5881 gcacggccgt cgtccacgac ggagagatcg tccacgcgct cacgcgtagc gccatggggc
     5941 tgccggaact cgccggggcg gcgcccgagc cggcgcccgc cgcggagcca cagacggtgg
     6001 aggagatgcc ggagccggaa cgggtggacg agccggaggg taccgaggag gcggtcatcc
     6061 acgagccggg cacgctcgag cccggcgaga ctgcggtgat cacgccggag agtgagccaa
     6121 cgacgactcc cgacgaggtg ttcgatgaca tcgacggtga gatcgacgac gagcccgagg
     6181 acgacgaccg tcggccccga tcggaacctg gagagaacgc atgaatgagg aattcctggt
     6241 tggaacatac gtgttcctgc tggccgtgtt cctaggggtg gaactcatcg gccgcgtacc
     6301 ttcgacactt cacacaccgc tgatgtcggc gaccaacgcg atccacggca tcgtcatgat
     6361 cggcggcatg atcctgctcg gcgacgtcga taatatcgcc ctgacgatcc tgggcttcgt
     6421 tgccgtggca ttcggggccg ccaatatcgt cggcggtttc gtggtgacgg atcgcatgct
     6481 tggtatgttc cggcgaaggc cgggcgccgc gagcggtggc gaggctgggt cacaacgatg
     6541 atcgagaacg gacgagaagc tgttatcgac gtcctgtacc tggcatcggc gatcctcttc
     6601 attctgtccc tcaggatgct gagcgcgccc agaagcgctc gcaacggcaa tctgctggcc
     6661 atggtcggca tggggctcgc cgtcctcgcc accttcttcc tcgaaggcgt gtttgacaag
     6721 atcatctgga tcctggtcgc gttcgccatc ggtggtggtg gtggctatct tgccgccaag
     6781 cgcgtcaaga tgaccgaaat gccgcagatg gtggcgatct acaacggtct cggcggcggc
     6841 accgtggccc tcgtctccgt cggcgagttc ctgagccgtg aggatctcgg ccgcgctacc
     6901 gaactcacga tcatcctcgg aaccatcatt ggcgcgatct cgcttatggg gagcatcgtt
     6961 gcattcgcca agcttcaggg gctgatttcc gggcgtccga tcaagctacc caaccagcag
     7021 ctctacaacg gtgcggcatt cgtcgcgatc gtcgtgctgg cggtcctcgt cctcgtcctc
     7081 ggtgagggcg ctctggacaa gtccttactg atcgtgatgt tcctgatcac gctggccatc
     7141 ggtgtggccc tggtgttgcc catcggcggt gctgacatgc ccgtcgttat cgcgctcttg
     7201 aacgccgcga cgggtcttgc cgcagccatc accggcttta ccatggacaa ccaggccctc
     7261 atcatcgccg gttcgctcgt cggtgcctcg ggtaccttcc tgagcatcct catggctcgc
     7321 gcgatgaatc gctcgctcgt gaacatcgtc ttcggcgcct tcggcgcggg cccagcagct
     7381 ggcgctggtc caggtgccgt cgaggcgggc gaggcgttgc cgatcaaatc gacaacggcc
     7441 gaggacgccg ccgtgacgct ggcatacgcc aacagcgtga tcatcgtccc gggctatggt
     7501 ctcgcggtgg cgcaggcgca gcacgcgaca cgagaactcg ctgatgtcat gcgcgagcgg
     7561 ggtgtcgagg tccgctatgc gattcacccc gtagcaggcc gtatgcctgg ccacatgaat
     7621 gtgttgctgg ctgaggcgaa cgtgccgtac gaggacttgt acgagatgga ccgcatcaac
     7681 gacgactttc gcaacacgga cgttgtgctc gtcatcggcg cgaacgacgt agtcaacccg
     7741 gcagccaaca acgaccccaa cagtcccatc tacgggatgc ctgtcctgca tgctaatgaa
     7801 gcgcgcaaca tcatcgtcct caagcgaagc atggacccgg gattcgccgg gatcgagaac
     7861 atgctcttcc acgacccgaa gacctcgatg ctcttcggcg acgccaagga ctcgctcaat
     7921 gagctcgtcg ccgccgtcaa gaacgtgtga tcctcgcgcc gggcatggag ttgtcctcac
     7981 gcccggcgtc gcatccccac gttgggcccg ggaggtggcc ggctatggca aatgatcccg
     8041 acacgcgctt cgacgacatc atgaaccgtc tcgagcaggc gtggggcctg tggcaggatg
     8101 cgatcggccg cgtgagcgag agcgatgcgc gagacgtcgc cctcgcccgc tcgctgatgc
     8161 acgagaccgc cgtgggcgat gagcaggcgg ttgaggtgat tcgctccctc gtcgagggaa
     8221 gcacctacca gcagttgcct ggtcgagaga cagttgagga ggctgacgac ctggatggcg
     8281 tgcgcagcat tatggagcat catcacgaga ggctgctcgg atccctcgat acggtcgccg
     8341 agaccgccga ggatatcgtg gtggctgtcg aggagcaggt ggggccgctc acctgggagc
     8401 agtatcctgt ccgtgcggag cgactgacca ccaccggcga cgccagcgcc tgacaaatgg
     8461 ggcgcctcac cctccttgcg tccctgctga tgccggcgat gttgctcgca ggctgcgtcg
     8521 ccctcccctc gctcaacgca caacctgcgg tctgcgttcc tcccgcccgc cacccttccc
     8581 tcgccaaggt cgatgggctc tacgtgctcc cggacgacgg tcgagccccg gtgcttgacg
     8641 agatcgaccg cgcctcctgc accatcgatc tcacgatcta cctcttgagt gacgacgcca
     8701 cgatcgctgc tcttgtccgt gccgaggctc ggggtgtgaa ggtccgtatc ctctacgaac
     8761 cggctccctt cggaggcggt gtcgggctcg tcgagacgac cgaagacctc gtccggcacg
     8821 gcgtcgagct gcgaagtgga tccgagggcg tcgcgctgat ccacgcgaag tacttggtga
     8881 ccgacaggtc gccatagtca cgaaccagaa cctggcgttc tcggcattcg agtccaaccg
     8941 ggagttcggt gtgatcacca ccgtgccggc cgacgtcgtg accctggcgt cgatcttcga
     9001 ggccgactgg agcaggttca cgccaacggt gtcgaccgac cgcatggtcc tgagcccggg
     9061 caacggccgg gcggcgctgc tcgaacagat tctcggtgcc gagtcaagca tccgtctcta
     9121 tgccgaggtg atccgcgatg acgaaatcat cgacgcgctt tcgacggtgg cgaagcgcgg
     9181 tgtgtcgtac agctcctggt gaacgagccg gaggacgatc tcgacgaaac ggtctacgac
     9241 gcgacgctca actcaaacgg cgtcgaggta cgccacacgg gcagcctcta cattcacgct
     9301 aagggactca ttgtcgatga gcaggccgtg gtagtcgggt cgcacaatcc gacagcgcaa
     9361 tcgctcgatc gaaaccggga agtttccctg gtgatcgagg gcgccccggc agtgaaacgg
     9421 ggggttgacg tgttcgatcg tgactggctg caagcgctcc ccgatcaatt tcgcaaaggg
     9481 ggttgacatg tcggtgctgt acggttattc tgtacgtaca gtcccgaggg ggattgaggc
     9541 ggatcagccg caaacgagat cgggtatcca agctgcgggg cgacgatacc ggcacgattt
     9601 gcgggcgcgg tgggtgaaga gctgacggct cacgttgcgg ggcgttgaac cgcacagcag
     9661 cacccgggtt cgctgatatc ccaccgggac tgtcgtttgt ttcactctct gagaagtccc
     9721 c
//
LOCUS       DASYWL010000171         5947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_8427, whole genome shotgun sequence.
ACCESSION   DASYWL010000171 DASYWL010000000
VERSION     DASYWL010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5947
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8427"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VGR22_06120"
     CDS             <1..621
                     /locus_tag="VGR22_06120"
                     /inference="COORDINATES: protein motif:HMM:NF014819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEV2128175.1"
                     /translation="LSRRNDAPEKASRPFDKGRDGFVLAEGAAVLVLEELAHAKAREA
                     SILGELIGYGMTDDANHMVQPAPEGAGAARAMALAFQDAEVGPADVGYINAHGTSTQL
                     NEKLETAAVKRAFGDDAKNVPISSTKSMTGHLLGAAGSLEAVISLKAMESGVLPPTIN
                     QMVPDPDCDLDVIPNQAREADVAVAMSNSMGFGGHNVSLIFRRWDE"
     gene            complement(715..2004)
                     /locus_tag="VGR22_06125"
     CDS             complement(715..2004)
                     /locus_tag="VGR22_06125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2128176.1"
                     /translation="MSREVAAAFTGARPAIVVVRLRIFLRIAPLDLMCRRLRCARPRG
                     GNASMDSSLLAKGKDQGYLLSEDIVAAYTNTDGAHESLADVYSALVAEGIDIVDSHPA
                     ATAPLLPERRTRKSRRAEVDEAIASNVGDSVRLYLQEIGETALLTMEEEVWLSTRMER
                     GLAAAERLNEPELSATERDDLLADKEDGDKARAHLIQANLRLVVSVAKKYVGRGLSFL
                     DLIQEGNIGLMKATDKFDYHRGYKFSTYATWWIRQAITRAISDQSRTIRLPVHVGETI
                     NRVKKTSYRLQQIYEREPTHEEIALAMAVSLDKVRQVLDVSRHPVSLEAPVGTEGDAL
                     LGDFIEDDSLVAPVDLAALGLLKGQICDTLTRLTERERKIVVLRYGLEDGRFRTLEEV
                     GKDFGITRERIRQIEAKALRKLRHPASSDRLRGYLDE"
     gene            2372..3118
                     /locus_tag="VGR22_06130"
     CDS             2372..3118
                     /locus_tag="VGR22_06130"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV2128177.1"
                     /translation="MSGHSKWSTIKRKKGAADAKRGQLFTKLAREITVAARQGLPDPD
                     ANVRLRLAVQKARAENMPKDNIDRAIQRASGGADSAQFDEVWYEGYGPGGVAVMILTM
                     TDNRNRTVAEIRAALTRSNGTLGETGSVNWMFDQMGVISLPTGDHDPDEIALVAIDAG
                     ATDVSDEDGTLEVYTEPQDLHKVQEELAAAGYEVESAELTMKPKTMVTAEQDEAVKAI
                     RLLEKLEDLDDVQTVYSNLDVTDEVLAAVS"
     gene            3123..3638
                     /gene="ruvC"
                     /locus_tag="VGR22_06135"
     CDS             3123..3638
                     /gene="ruvC"
                     /locus_tag="VGR22_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEV2128178.1"
                     /translation="MPEPSARVTIGIDPGTAILGYGVVAGDDRIEVLDFGVVETGSKQ
                     DMPERLRILHESMTGLLERHQPDELAVEQLLFGRNVTTAISVGQARGVVLLAAAQRGV
                     PVFEYSPAEVKNAIVGYGKADKRQMQEMVRILLELSEVPHPDDAADALAVALCHAQTR
                     QFSSHLDSGRQ"
     gene            3635..4447
                     /locus_tag="VGR22_06140"
     CDS             3635..4447
                     /locus_tag="VGR22_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEV2128179.1"
                     /translation="MTRGDTRLDIAMVERGLVENRSRAKAMILAGDVLVDGEAVSRAG
                     AVVGAQSNISLKHKPQFVGRGGEKIDHALNRFDIDVAGKVVADLGACTGGFTDAVLQR
                     GAVRVYAIDVGYGQLDFRLREDPRVVVMERTNARYLEALPDPIDVCMIDVSFISLNLM
                     FPAVDRILAAAGVVVPLIKPQFEAGKGEVGKGGVVRDPAIHRSVLAKVVRQASEHGFR
                     CEALTASPLTGPAGNHEFLGLFRRSEEPVDEDELTAMIDGALGEVRRQEEET"
     gene            4444..5223
                     /gene="larB"
                     /locus_tag="VGR22_06145"
     CDS             4444..5223
                     /gene="larB"
                     /locus_tag="VGR22_06145"
                     /inference="COORDINATES: protein motif:HMM:NF033503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HEV2128180.1"
                     /translation="MSDPFADVVMALQREGDGLDRDGEGVRVDAYRQTRTGIPEVVFA
                     SHKRLDALVASIARLLDANGRAMVSRIQSDQWETVARFERDGFEVERFEAAAVAIIRR
                     SCSQVPVTGGRIAVIAAGSSDRAVAEEAAVMAQEMGVEVFRATDVGVAGLHRLVRPLR
                     DIAERDADCIVVAAGMDGALPSVVAGLVDVPVIGLPVSVGYGHGGNGEAALMTMLQSC
                     APGITVVNIDNGIGAGSTAALIANRVAAARDGRHPMAEIPV"
     gene            complement(5272..5478)
                     /locus_tag="VGR22_06150"
     CDS             complement(5272..5478)
                     /locus_tag="VGR22_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128181.1"
                     /translation="MQTLAIITLLTLAALAVVPLTIGYYLWLDRAILPRVLKLPTRIA
                     RTILALLIALPWVVAALTVWLLRG"
     gene            5652..>5947
                     /locus_tag="VGR22_06155"
     CDS             5652..>5947
                     /locus_tag="VGR22_06155"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEV2128182.1"
                     /translation="MTNQKPVRKNYDYANLAGADLSEADLSHASLRRAILTDASLRNA
                     NLAGSDLHGANLDHADVTGANLRNADLTDASLLGVDLSRAAATEGVRLAGAEAP"
BASE COUNT         1080 a         1866 c         1996 g         1005 t
ORIGIN      
        1 ctctcgcgcc gtaatgacgc gccggagaag gccagccgtc ccttcgacaa ggggcgcgat
       61 ggcttcgtgc tcgccgaggg cgcagcggtg ctcgtcctcg aggagctggc gcatgcgaag
      121 gcgcgagagg cgtcgatcct cggcgagttg atcggttacg ggatgaccga cgacgccaac
      181 cacatggtcc agccggcgcc ggagggcgcg ggcgcagcca gggcgatggc gctggccttc
      241 caggacgccg aggtgggacc tgccgatgtc ggatacatca acgcccatgg aacctcgacc
      301 caactcaacg agaagctgga gacggcggcg gtcaagcggg cgttcggtga cgacgcgaag
      361 aacgtgccga tcagctcgac gaagtcgatg accggacacc tgctgggcgc ggctggttcg
      421 ctggaggcag tgatcagcct caaggcgatg gagagcggcg tcctgccacc gacaatcaat
      481 cagatggtac cggatcccga ctgcgatctc gacgttatcc cgaatcaagc gcgggaggca
      541 gatgtcgcgg ttgccatgtc gaattcgatg ggcttcggcg gccacaacgt ctcgctgatc
      601 ttccgtcgct gggatgagta ggctgagggc ttccgttctg gcatgaaggc ggcagaaaga
      661 acacccccgg ccacgggcgg ccgggggtgt tctttctcgt gttgtccatg gccgctactc
      721 gtcgagatat ccacgaaggc ggtcgctcga ggccgggtgg cggagcttgc gaagcgcctt
      781 ggcctcgatc tgccggatgc gctcgcgggt gatgccgaag tccttgccga cctcctcaag
      841 ggttcggaag cggccgtcct ccaggccgta ccgcaacacg acgatcttgc gctcgcgctc
      901 tgtcagccgg gtgagggtgt cgcagatttg ccccttgagc agccccagcg cggcgagatc
      961 gaccggcgcg acgagcgagt cgtcctcgat gaagtcgccc agcagcgcgt cgccctctgt
     1021 cccgaccggc gcctccagcg agaccgggtg ccgcgacaca tcgagtacct ggcgaacctt
     1081 gtcgaggctg accgccatcg ccagtgcgat ctcctcgtgc gtcggctcgc gctcgtagat
     1141 ctgctgcagt cggtacgacg tcttcttgac ccggttgatc gtctcgccga cgtggaccgg
     1201 aaggcggatg gtgcggctct gatcggagat cgctcgagtg atcgcctgcc gaatccacca
     1261 tgtcgcgtag gtgctgaact tgtagccccg gtggtagtcg aacttgtcgg tggccttcat
     1321 caggccgatg ttgccctctt ggatgagatc gaggaaagag agcccgcgac cgacgtactt
     1381 ctttgccacg gagacaacga gccggaggtt cgcctggatc agatgggcgc gcgccttgtc
     1441 gccgtcttcc ttgtccgcga gcaggtcgtc acgctcggtt gcggagagct ctggctcgtt
     1501 gagccgctcg gcggcggcga ggccgcgctc catccgagtc gagagccaga cctcttcctc
     1561 catcgtcagc agggcggtct cgccgatctc ctgcaaataa agccgcaccg agtccccgac
     1621 gttcgacgcg atcgcctcgt cgacctcagc gcgccgcgac ttgcgggttc gccgctcggg
     1681 caggaggggc gcagtcgccg cggggtggct gtcgacgata tcgatgccct cggcgaccag
     1741 cgccgagtag acgtcggcaa gcgactcatg ggcgccatcc gtgttcgtat acgccgccac
     1801 aatgtcttcc gacaacaggt atccctggtc tttccctttt gcgagtaggc tcgagtccat
     1861 tgaagcgttt ccacctctcg ggcgagcgca gcgcaatcgt cggcacatga ggtcgagcgg
     1921 ggcgatgcgc agaaagatcc gaaggcgaac gacaacgatc gcgggtcgtg ctccagtgaa
     1981 ggcggcggcg acttcacgtg acatcgactc cagccggcac aagcccccga aaacagaatg
     2041 acccctcgct ccaggcgacg ggccgtcagt ttcgagctat ggcattgtgg cggacatggc
     2101 acacatgtgg aagcggttcc ccccagcgtc tgtcgctcga ctgctaaaca catggtagtc
     2161 cacggcaaca cgcggattca agggaaacta tacagtctgc agaatactgc gagccggaat
     2221 ggtctttggc aggacccaag gagatgttcg agcggcccaa cgaccgtgac ggccaccacg
     2281 aaccgctgca actcgggtga tatactcggc cgttggcaga tattgcgccg atcccgtggc
     2341 gcccggcacg acgccaggag agaactctcg tatgtccgga cattccaagt ggtccaccat
     2401 caagcgcaag aagggtgctg ctgacgccaa gcgcggccaa ctcttcacca agctcgcccg
     2461 cgagattaca gtcgcagcgc gccaggggct acctgatccc gacgcgaatg ttcgcctgcg
     2521 tctcgcggtc cagaaggcgc gtgccgagaa tatgccgaag gacaacatcg atcgggcgat
     2581 ccagcgcgcc agcggcggag cggacagcgc gcagttcgat gaggtctggt acgaggggta
     2641 cggtcctggc ggagttgcgg tgatgatcct caccatgacc gacaaccgca atcgaaccgt
     2701 cgccgagatt cgcgccgcgt tgacgcgctc gaatggcacc ctcggagaga ccggcagcgt
     2761 gaattggatg tttgaccaga tgggcgtgat cagtctgccg accggcgatc acgaccccga
     2821 tgagatcgct ctggtggcga tcgatgccgg tgctaccgac gtctccgatg aggacggcac
     2881 cctcgaggtc tacaccgagc cgcaggacct ccacaaggtg caggaggaac tcgcggcggc
     2941 aggctacgag gtcgagtcgg ccgagctgac gatgaagccc aagaccatgg tcacggcgga
     3001 gcaggacgag gcggtgaagg cgattcgcct actcgagaag ctcgaggatc tcgacgacgt
     3061 gcagaccgtt tacagcaacc tcgacgtcac ggacgaggtg ctcgccgcag tcagctagcc
     3121 ggatgcccga accatctgct cgcgtcacaa tcggcatcga tcccggcacc gcaatcctcg
     3181 gatatggtgt tgtcgctggc gacgaccgga tcgaggtgct ggatttcggg gtcgtcgaga
     3241 ccggctccaa acaggacatg ccggagcggc ttcggatctt gcatgaatcg atgactggcc
     3301 tgcttgagcg tcaccagcct gacgagctcg cggtggagca gcttctgttt gggcgcaacg
     3361 tcacgacagc gatctcggtc ggacaggcgc gcggcgttgt gttgctcgcg gcggcgcagc
     3421 gaggtgtgcc cgtgttcgag tacagcccgg ccgaggtcaa gaatgcgatc gttggctacg
     3481 gcaaggcgga caagcggcag atgcaggaga tggtgcggat cctgctggag ctctccgagg
     3541 ttcctcaccc cgatgatgct gccgacgcgc tcgcggtcgc actctgccac gcacagacgc
     3601 ggcagttcag ttcccatctg gacagcggta ggcaatgaca cggggcgaca cgcggctcga
     3661 catcgcgatg gtcgagcgtg gtctggtcga aaaccggtcg cgggcgaagg caatgatcct
     3721 tgcaggcgac gtgctggtgg acggtgaggc agtgagccgc gccggtgcgg tcgtcggtgc
     3781 gcaaagcaac atatccctga aacacaaacc ccagttcgta ggcagaggcg gcgagaaaat
     3841 tgaccacgcc ctcaatcgtt tcgatattga cgtggccggg aaggtggtcg cggatctcgg
     3901 ggcctgcacc ggcggattca cggatgccgt gctccaacgc ggtgcggtga gagtctatgc
     3961 aatcgatgtc ggttacgggc agctcgactt ccgactgcgc gaggacccgc gcgtcgtcgt
     4021 gatggagcgc acaaacgccc ggtacctgga ggcgctgcca gatcccatcg atgtctgcat
     4081 gatcgatgtc tccttcatct cgctcaatct catgttcccg gccgtcgacc ggatcctcgc
     4141 cgccgccggt gtggtggtgc cgctgatcaa gccacagttc gaggccggaa agggtgaagt
     4201 cgggaagggc ggcgttgttc gcgacccggc catccaccgg tccgtgctgg caaaggtagt
     4261 gcgccaggca agcgagcacg gattccggtg cgaggcgttg accgcctcgc cgctgaccgg
     4321 tcccgccggc aaccacgagt tcctcggcct cttccggcgg tcggaagaac cggtcgacga
     4381 ggacgagttg accgcgatga tcgatggcgc cctcggcgag gtccgccggc aggaggagga
     4441 gacatgagcg atccattcgc agacgtcgtg atggccctgc agcgcgaagg cgatgggctg
     4501 gatcgagacg gagaaggtgt tcgcgtggat gcttatcggc agacacgcac cggcatcccg
     4561 gaggtcgtct tcgccagcca caagcgcctg gatgcactcg tcgcctcgat cgcgcgcctg
     4621 ctggacgcca acgggcgggc gatggtttcc cgcatccaat cagatcaatg ggagacggtc
     4681 gctcgattcg agcgggacgg cttcgaggtc gaacgctttg aagccgcggc agtggcgatt
     4741 attcggcgct cctgcagcca agttcccgtg accggcgggc gcattgccgt gatcgcggcg
     4801 ggcagctccg atcgcgcggt ggccgaggag gctgccgtca tggcgcagga gatgggtgtc
     4861 gaggttttcc gggcgaccga cgtcggtgtc gcggggctgc accgcctggt acggccactg
     4921 cgggacatcg ccgagcgcga tgccgactgc atcgtggtcg ccgccggcat ggacggcgcg
     4981 ttgccgtccg tcgtcgctgg cctggtcgac gtgccggtga ttggtctgcc ggtgagcgtc
     5041 ggctacgggc acggcggtaa cggagaggcc gctcttatga cgatgctcca gagttgcgcg
     5101 ccaggcatca ctgtggtcaa catcgacaac ggcatcggcg ccggttcaac ggcggcgttg
     5161 attgcgaacc gggtcgccgc cgcccgcgat ggtcgccacc caatggcgga aatccctgtg
     5221 taggatcgtc ctcgggccag gcattccatc cgtggtgccg tgccccatcc atcatcctcg
     5281 gagcagccag acggtgagcg cggcaacaac ccatggcagg gcaattaaga gcgcaagaat
     5341 ggtccgggcg atccgcgtcg gcagcttgag taccctcggc aggatcgctc gatccaacca
     5401 gaggtaatag ccaatcgtga gcgggacgac cgccagcgcg gccagcgtca gaagggtaat
     5461 gatggcaagg gtctgcatct gtcctcctgc tgggccaacc cgcgtcgctc cccgcgctcc
     5521 ctgccccgaa catggcaccg aacctgcacg acccgccgag ggatactacc gaatcggggc
     5581 aactgccttg gttccgcccc ccacccgatg ctcaccgccg gatgtggcgg tcggaccagg
     5641 aggtgctgct catgaccaat cagaagccgg tgcgcaagaa ctacgactac gccaatctcg
     5701 ccggtgcgga cctgtcagag gccgacctgt ctcacgcctc gctgcggcgc gcgattctca
     5761 cagacgcgtc cctgcgcaac gcgaacctcg ccggaagcga cctgcacggg gcgaacctcg
     5821 accatgccga cgtgaccggc gccaacctgc gcaacgccga cttgaccgac gccagcctgc
     5881 tcggagtcga tctcagccgg gcggcagcga ccgagggggt tcggctcgct ggggcagagg
     5941 cccccag
//
LOCUS       DASYWL010000172         4931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_64433, whole genome shotgun sequence.
ACCESSION   DASYWL010000172 DASYWL010000000
VERSION     DASYWL010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64433"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..300
                     /locus_tag="VGR22_06160"
     CDS             85..300
                     /locus_tag="VGR22_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128183.1"
                     /translation="MRHDKQMMGGYSKLTRALALSPVTIYLLTMVFVAEWNHYYLSLS
                     NVYMATVMVAPMGSTMLLVMRRMFPNR"
     gene            complement(624..833)
                     /locus_tag="VGR22_06165"
     CDS             complement(624..833)
                     /locus_tag="VGR22_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128184.1"
                     /translation="MPKQWGDLSKEEQEKFQNMDISGEEGGDTGPSGHIDDGEQAEKG
                     DTRRENPKHSGPTRNGPAGNEKQNS"
     gene            complement(950..1042)
                     /locus_tag="VGR22_06170"
                     /pseudo
     CDS             complement(950..1042)
                     /locus_tag="VGR22_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008745198.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1149..1298
                     /locus_tag="VGR22_06175"
     CDS             1149..1298
                     /locus_tag="VGR22_06175"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEV2128185.1"
                     /translation="MRAVIPHHSCAILVCEESSITDPDIEELCQRIISTQQEEIRIMK
                     DMLGE"
     gene            complement(1345..2268)
                     /locus_tag="VGR22_06180"
     CDS             complement(1345..2268)
                     /locus_tag="VGR22_06180"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEV2128186.1"
                     /translation="MTTGMPIVSTLLGVVLIAFVLRDIFHTLFHPSGIGTLSSTGARY
                     LWRVLRQFARHHRQVLPLAGPLALVAIIATWALVLVLGWALIYWPHLTEGFLLATGLE
                     PDDNDSFLEAFYFSLVTLGTLGHGKITPLTPWLRIVSPLQALVGFALFTASISWILSV
                     YPVLGRRRQLAREVALLRRGRSAPGLRTVEDAPAALEGTLLSLAEQVNAICGDLEQFP
                     ITYYFHLPDEQSALPVALPDLVALAREGQHHGSPAVRLQSTLLLEAIHDLAGHVGETF
                     DRRGADLGDALAAYAVDHFYLAPEPAQAASQ"
     gene            2453..2734
                     /locus_tag="VGR22_06185"
     CDS             2453..2734
                     /locus_tag="VGR22_06185"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEV2128187.1"
                     /translation="MRRTHYSEDRGVLQVRLRKMEGQVRGLQQMIDDDRYCLDVVQQV
                     NARTSAAREVTLIGMENHLRATVAEAVRDQDGEAAIREMVDVMRKALRA"
     gene            2875..3219
                     /locus_tag="VGR22_06190"
     CDS             2875..3219
                     /locus_tag="VGR22_06190"
                     /inference="COORDINATES: protein motif:HMM:NF015798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HEV2128188.1"
                     /translation="MPHQLSTEMRDCISNCTDCHNVCVETANHCLEMGGKHAEASHIR
                     ELLDCAQICGTSADFMLRGSDLHSQTCGVCADACERCAESCERLAEADELMQRCAGIC
                     RRCAESCRQMAA"
     gene            3642..4409
                     /locus_tag="VGR22_06195"
     CDS             3642..4409
                     /locus_tag="VGR22_06195"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2128189.1"
                     /translation="MRIGVEEDSYRTDERANVGFYPLNTNIFESLVYMTPDYEIEPML
                     AESWEFVEPNTWRISLRQGVKFHDGTDFTAEAVKYTIDRLAANGGGSIGVAENSTVIV
                     DDYTVEITPSFTNRRMMQQIGHPNNSILPVGLNPAETRIGTGPLREVEYVAGDRYTVE
                     ANPDYWGEAPGVNEITFQFYPDPTARLLAIQAGDVDMITDVPRQSASQVEAIGASLIT
                     GPVGAYEAMYIQIHGEEPYDLGSEFAVREAVSMAINR"
     gene            4456..>4931
                     /locus_tag="VGR22_06200"
     CDS             4456..>4931
                     /locus_tag="VGR22_06200"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2128190.1"
                     /translation="MIPPAILGDYADLVEAPQYDPEAARQVLEEAGWVEGEGGVRERD
                     GRRLTLTLINGYGSAADHGSVPELLQAQLGEIGVEVEIVTTPDTATYEQRLAVGEGDL
                     WLEAGSQNDANPAFLPGLLFYNPEEGGDPEATMYDRAFAPGEEFDTHIDRAREAVET"
BASE COUNT          911 a         1572 c         1606 g          842 t
ORIGIN      
        1 ggacgcgggg aggggtcccg ggctcattga cccatgactt cgctccgatg ccggaaatgg
       61 atcccctcga taagctcgca ggagatgcgg cacgacaagc aaatgatggg cggctactcg
      121 aagctgaccc gcgcgctggc gctcagcccg gtgacgatct acctgcttac catggttttc
      181 gttgccgagt ggaaccacta ctacctcagc ctcagcaacg tctacatggc cacggtcatg
      241 gtggccccga tgggctcgac catgctgctg gtcatgcgca ggatgttccc gaacaggtga
      301 gtcaacgcgg tcctcacaat cggctttgtg gcgctctttt tcgccgcgtt ctggacgggg
      361 cgctcgcagg cgttcgttgg caactaagag cctgcgccaa tagggcgagc gcgctcggca
      421 gtatcatcca caacagcctc atcggcagcc aacgatcggt taccagtccc gtccgcgcct
      481 attggcgcaa gctccaagag aatcgcaagc gtgacaaggt gatcaggtct ccgtcacagg
      541 ggaggccaca tgggaatgat tgatcacgat cgtcatgcca tgcgtaacct ccggagtctg
      601 gccaccttca ccacaccagg ggatcaggaa ttctgcttct cgttcccagc gggtccgttg
      661 cgcgtcggtc cgctgtgttt aggattctcg cgacgggtat cccctttctc ggcctgctcg
      721 ccgtcgtcaa tgtgtcccga gggtccggta tctccaccct cttcgccgct gatgtccata
      781 ttctggaact tctcctgctc ttccttgctc aggtcgcccc actgctttgg catagttgtc
      841 ctccttattc tcggcgcagg atacgctggc ggttgccatg cagtttctat tccagggttc
      901 accgaccgct gcgcgagcta ggtgacgcgg tccggcacgg ggcagggcgg cacccgagcg
      961 gatggatcac cgagtggcat ggcaggagcg cggcgaactg gtccgagatg ggctcgctga
     1021 tgggcggcgg gatggcgggc acgggagcgc tcctcgcacg aaacgcctga acaccttccc
     1081 gtatgatcaa gcaccccgtg cctcacgtac cgtcgacgac tgtctatggg cgcaagctct
     1141 aactgttcct gcgggccgtg atcccgcacc attcgtgcgc gatcctggtt tgtgaggagt
     1201 cctcgatcac cgatccggac atcgaggagc tgtgccagcg gatcatctcc acccagcagg
     1261 aggaaatcag gatcatgaag gacatgctgg gcgagtgagc gatgcgccat ggtgcaccga
     1321 gcgccccttg ggcaatgcag ctcttcactg agatgccgct tgggccggtt ccggtgcaag
     1381 gtaaaaatgg tctacggcat aggcggcgag tgcgtcgccc agatccgcac cgcgtcgatc
     1441 gaaggtctcg cccacgtgcc ccgcgagatc atggatggcc tcgagcaaca gggtgctttg
     1501 cagccggact gcaggcgatc cgtggtgctg cccctcccgg gcgagtgcca caaggtccgg
     1561 gagggccact ggcagggccg attgttcgtc tggcaggtga aagtagtagg tgatgggaaa
     1621 ctgctcgagg tccccgcaga tggcgttcac ctgttccgcc aggctgagca gcgtgccctc
     1681 cagcgccgca ggcgcatcct ccacggtgcg gagccccggc gctgatcgcc cccgtcgaag
     1741 cagggcgacc tcccgggcca gttggcgccg gcggccgaga acggggtaga ccgagagaat
     1801 ccacgagatc gaagcggtaa agagggcgaa ccccaccagc gcttgcaacg ggctgacgat
     1861 tcgcagccac ggggtcagtg gcgtgatctt gccatggccg agcgttccca gcgtcacgag
     1921 ggagaagtag aacgcctcca gaaagctgtc gttgtcgtct ggctccaggc cggtggcgag
     1981 gaggaacccc tcggtgaggt gcggccagta gatgagtgcc cagccgagca cgaggaccag
     2041 tgcccacgtc gcgatgatcg cgaccagcgc cagagggccg gccagcggca ggacctgacg
     2101 gtgatggcgg gcgaactggc ggaggacccg ccagaggtag cgtgcgcccg tgctgctgag
     2161 ggtgccgatc ccgctcgggt ggaacagcgt gtgaaagata tcgcgcagca cgaacgcaat
     2221 caggaccaca cccaagagcg tggagacgat gggcattccg gtggtcatag cgcgctcttc
     2281 ccaaaggttg cgagacgcgg agccgtgccc ataccttgta cggtccaagc ccatcccgca
     2341 tcggcccctc atggcggggc tgcttgagcc agtgacgttc tcccttgcgc aatgcccgtg
     2401 ccgttcctcc atactggagc gagcaatcgg cacgcgtgag ggaacgatag acgtgagacg
     2461 gacgcactac agcgaggatc ggggcgtgct ccaggtccgc ctgcgcaaga tggagggcca
     2521 ggttcgcgga ctgcagcaga tgatcgacga tgaccggtac tgcctcgatg tggtccagca
     2581 agtcaacgcg cgtacctcgg ctgcgcgcga ggttacgctc atcggcatgg agaaccacct
     2641 ccgcgccacg gtcgccgaag ccgtcaggga tcaggacggg gaggccgcca ttcgggagat
     2701 ggtcgacgtc atgcgtaagg cactgcgagc atagattcct ctggacggag cgtaaggggc
     2761 atagacaact tctgtactgg gtaccggtag acagtagcgg catgttgctt gcggttccac
     2821 ccgatagcac ccaagggtgc tgctgacgcc gccgacaacg gtaggagatg tctcatgcca
     2881 catcagctca gcactgagat gcgcgactgt atcagcaact gcacggattg ccacaatgtc
     2941 tgcgtcgaga cggcgaacca ctgcctggag atgggcggca agcacgccga ggcgagtcat
     3001 atccgggaac tgctcgactg tgcccagatt tgcgggacga gtgccgactt catgctgcgt
     3061 ggctcggacc tccatagtca gacatgcggc gtctgcgccg acgcctgcga acggtgcgcg
     3121 gagagttgcg agcgcctcgc cgaggccgat gagctgatgc agcgctgcgc cgggatctgc
     3181 cgccgctgtg ccgagtcgtg ccggcagatg gccgcgtagc gtcgtccatt ctcgcccctc
     3241 gatccctcgt atcgttcatc ccagccaccg gctctgaaga ctgctcagag ccggtggctg
     3301 agtgttttcc cagagatgat gcgtcacgcc cggcactggc gttgccacgt gttcgggcag
     3361 tctcctgttg gaatccagac ctctgtcgtc ctggatcgga catggtcgct ggataccccc
     3421 tgcacgtatc ggccgagata tgccagaata gtgcaacaca ccaacggagg agtatcgcgc
     3481 ttcgcttccg gccacccgag ggtcgccgag ccacgaaagg accacgcatg ggaacgctgt
     3541 cacgcaggat cgatcgacgc acgctcgtgc tcgccaccgg cgcgacgggc atttccctct
     3601 cgctgctgtg aagcgtcgcg gcacagaatg gcaccggctc cgtgcgcatc ggcgtggagg
     3661 aggatagcta ccgcaccgac gagcgcgcca acgtcgggtt ctacccgctg aacacgaaca
     3721 tcttcgagtc gctcgtctac atgaccccgg actacgagat cgagccgatg ctcgccgagt
     3781 cgtgggagtt cgtggagccc aacacgtggc gcatctcgct gcgacagggc gtgaagttcc
     3841 acgacggcac cgatttcacc gccgaggcgg tcaagtacac gatagaccgc ctcgccgcca
     3901 acggtggcgg ctccatcggc gtcgccgaga actcgacggt gatcgtggat gattacaccg
     3961 tcgagatcac tccgtcgttc actaaccggc ggatgatgca gcagatcggc cacccgaaca
     4021 actcgatcct gccggtcggt ctcaacccgg ccgagacgcg catcggcacc ggccccctcc
     4081 gtgaggtcga gtacgtcgcc ggggaccgct acaccgtcga ggcgaatccc gactactggg
     4141 gcgaggcgcc gggcgtcaat gagatcacct tccagttcta cccggacccc accgctcgcc
     4201 tgctcgccat tcaagccggc gacgtcgaca tgatcaccga tgtaccgcgg cagtccgcca
     4261 gccaggtgga ggcgatcggc gcgtcgctca tcaccggacc ggtcggcgcg tacgaggcga
     4321 tgtacatcca gatccacggc gaggagccgt acgacctcgg gagcgagttt gccgtgcgcg
     4381 aggctgtctc gatggcgatc aaccgctagc aggtcatcga cagcgcctgg cagggcaacg
     4441 tggaggcggg caccgatgat cccgcccgcg atcctcgggg actacgcgga cctggtcgaa
     4501 gccccgcagt acgaccccga ggccgcccgc caggtcctcg aggaagccgg ctgggtggag
     4561 ggcgagggcg gcgtccgcga gcgggacggc cgccggctca cgctcacgct gatcaacggc
     4621 tacggctcgg ccgcggacca cggatccgtg ccagagctgc tccaggcaca gctcggcgag
     4681 atcggcgtcg aagtcgagat cgtcacgaca ccggacaccg cgacctacga gcagcgcctc
     4741 gcggtcggcg agggtgacct ctggctggag gccggcagcc agaacgacgc caacccggcg
     4801 ttcctgcccg gtttgctgtt ctacaacccg gaggagggtg gtgacccgga agcgaccatg
     4861 tacgaccgcg ccttcgcccc cggtgaggag ttcgatacgc acattgaccg cgcccgcgag
     4921 gcggtggaga c
//
LOCUS       DASYWL010000173         2210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_45304, whole genome shotgun sequence.
ACCESSION   DASYWL010000173 DASYWL010000000
VERSION     DASYWL010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45304"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VGR22_06205"
     CDS             <1..470
                     /locus_tag="VGR22_06205"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEV2128191.1"
                     /translation="NCLPYAGLLTVFGQGVRAARPGEAGRRTGGLLVAYGLANAIGGV
                     VFPMAQRPVLAANAHSPINAMHIPVMGIMSAFLLLALRSASVLSGKGVRAFTNVTTVA
                     LVLPAVLMAPDVPKVARNEPTPYMGALARINIYATALWIAVLGLVLMRDEEER"
     gene            709..1149
                     /locus_tag="VGR22_06210"
     CDS             709..1149
                     /locus_tag="VGR22_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128192.1"
                     /translation="MAWRPGGRTPYFHKRSALPLLRPGGRVLVTVAFALAGSLPLAMF
                     WTRDLLGNLYHPVRQAWMNRNIEPRTRATVLSFQEQIASMGEIGGGPPLGWVGSRFGV
                     RAAIVAAGLVFSPVIAVFLRAAHLRDHVAGPHAPIMESPPAELA"
     gene            complement(1882..>2210)
                     /locus_tag="VGR22_06215"
     CDS             complement(1882..>2210)
                     /locus_tag="VGR22_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2128193.1"
                     /translation="DAAFDRAFQGGQELALDAAVTESLALAQMISVAQIPPPSDRIEY
                     PAGLSAREVEVLQLVAVGMTNAQVADRLFLSRRTVDAHLRRIYDKLDLSSRTEAIRFA
                     IEHGLA"
BASE COUNT          328 a          776 c          719 g          387 t
ORIGIN      
        1 cgaactgtct cccctatgcc ggcctgctga ccgtctttgg ccaaggcgtg cgcgctgcaa
       61 ggcccggcga ggccgggcgc cggacggggg gcctgctggt cgcctacggc ctcgcgaacg
      121 cgatcggtgg ggtcgtcttc cccatggcgc agcggccggt cctggcggcg aatgcgcatt
      181 cgccgatcaa cgcgatgcac atccccgtga tggggatcat gtccgcgttt ctgctgctcg
      241 cgctgcgctc tgcctctgtc ctctcaggca agggggtccg cgcgttcacg aatgtgacca
      301 ccgttgccct cgtcctgccc gctgtgctga tggcgccgga cgtgccgaag gtcgcccgga
      361 acgagccaac cccatatatg ggcgcgctcg cgcgcatcaa catctacgcc acggcgctct
      421 ggattgccgt gctcgggctc gtcctgatgc gcgatgagga ggagcgctaa tcccgcgtca
      481 agtcaacctt cgtggcgcac gtgcccacgg tcgcggcgtg agagcgcgcc cgcgccggcg
      541 tgcgtgactc caacgatcat tgaccgcgcg taaggcatcc ccccgagcgc ggccacgctc
      601 atgcctgatt tcgcgcacta gacagcggcc gggtgctcct ttcccccggc cgtttgtcgt
      661 tggcatcgat cctgcatccg tgaccagcaa cgtcaccggg gcgttgtgat ggcatggcgt
      721 cccggtgggc gtactcctta cttccacaag cgctcggctc tccctctgct gcggccgggc
      781 ggccgcgtgc tcgtcacggt ggccttcgca ctggcgggat cgctcccgct ggcgatgttc
      841 tggacgcgtg acctgcttgg caacctctac cacccagtcc gccaggcatg gatgaaccgc
      901 aacatcgagc cccgcactcg cgcgacggtg ctctccttcc aggagcagat cgcctcgatg
      961 ggcgagatcg gcggtggccc gccgctgggc tgggtcggca gccgattcgg tgtccgcgcc
     1021 gcgatcgtcg ccgccgggct cgtcttctcc ccggtgatcg ccgtcttcct ccgggccgcg
     1081 cacctgcgag atcacgtcgc cgggccccat gcgcccatca tggaatcgcc acccgccgag
     1141 ttggcgtaag cctgagcgcc acggtggtca ccgatcgatc cacctcatcc agaagggtgg
     1201 acaccctacc gggttccgcc cttcgacgcc gttctgtgaa gatgtccatg gcgacggtaa
     1261 catgtccgtg cacccgatgc gctacgctct aggcatgccg cgtgatatca gcaccatctc
     1321 ggattcccca ctcttggggt gctgcgcgtg gtgccgagcg gcgtcacgat cggcaccagc
     1381 accaccgtca cgaacgggtt ccgatacgtg agccatcagg ggcagattgg ctggatggcc
     1441 gatgcctaca tcagcgccac caaccccgac gaccaggcag aagggcaccg cgtccttcgt
     1501 ggcggagcgc aatcctcacg tccgtgcagg tccgccccct ggtatccgga tggtcactgc
     1561 ccaggaacgg cggcagtcgg gttcacaggg gcgcgccgtc acgacagaac gcatcagtcg
     1621 aggttggggc caaccgcgcc cacgtcggcg tcggcgcaga ccgaccatac ccgccaccac
     1681 atgctcctcc cgtcgctgcg ttggcgtgac ggcggcccgg ggctgcccct caccccactc
     1741 gtcgggcgcg accatgaggt cgccacgatg gctgcgctgc tgggcaagcc ggaccatcgt
     1801 ctggtcaccc tttacacgtt gccaggcagg acctgaccgg agcacgtgtg tacgtgctgg
     1861 ttggcatggg gaagggcggg ctcacgcaag cccgtgttca atggcgaacc gaatcgcctc
     1921 ggtcctcgag gagagatcga gcttgtcgta gatgcgtcgg agatgggcgt cgaccgtgcg
     1981 ccggctgagg aagagccgat cagccacttg cgcattggtc atcccgactg ccacgagctg
     2041 cagcacctcg acctcccgag ccgacagccc tgccgggtac tcgatcctgt cgctcggagg
     2101 ggggatctgc gcgacggaga tcatctgggc gagcgcgagg gattccgtga ccgcggcgtc
     2161 gagcgcgagc tcttgccctc cctggaaggc gcgatcgaag gcggcgtctc
//
LOCUS       DASYWL010000174         4735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_383552, whole genome shotgun sequence.
ACCESSION   DASYWL010000174 DASYWL010000000
VERSION     DASYWL010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383552"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VGR22_06220"
     CDS             complement(<1..208)
                     /locus_tag="VGR22_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128194.1"
                     /translation="MTRYCPLGDGAFDDWVASCPECGSRLVDTPPNPDDLEASATNAS
                     PVLLTTAPNEIEANLIAGILRDEGI"
     gene            437..637
                     /locus_tag="VGR22_06225"
     CDS             437..637
                     /locus_tag="VGR22_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128195.1"
                     /translation="MQEISRIITALGAVVASTGLVVYGMGGSYWTPGDFELNLGVWMM
                     VLGVIATIVGIILYQRTWVEHD"
     gene            complement(702..2306)
                     /locus_tag="VGR22_06230"
     CDS             complement(702..2306)
                     /locus_tag="VGR22_06230"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV2128196.1"
                     /translation="MTVDSVWHSDLFGRDELNGDVLLDFLRATEAADDSREPFDAFDQ
                     ALTRLVTIDRLSISCLDRRTDRATVIYERGISLSTAAQHHSVPATFPALVHQAVQENR
                     PVYHTIPDHDDVDRQDGPRRQAALRQALAAPVFVDADTAAVLVLSCTSPAPFSTAEAR
                     VVETFARVLGLALASHDISETTGHKETSREISRTIDRLMHTSSTTQLLDELQRVVIAI
                     TDATVLLFQLDHRNVRFVEARNPQPDLLMGQSLDDVGEQITSRVAELLAGGLSPGCAT
                     TLFTDPRAIGDAGVPVRTMDACHDLLAINAAAISVLDGHETSMWAMIAAWPETVSPVA
                     AARHAESIDRLVRPAGALMQRLARMESLEQQVVEMDITCRPMNAVARTPRLQDAFEVI
                     CRTSQLVTNLDFVAIAETVNDHVFWHAATGAMDPSFLGTRVPVPPRILRDFLQHSFEV
                     LLEDVHKEPDMTPEIMPLHTRERLRSTVILPVHVNARPRGAMVASQRHLHTFSPAELS
                     LLHHLAGAVATAIAANDARLAEGENE"
     gene            complement(2303..3751)
                     /locus_tag="VGR22_06235"
     CDS             complement(2303..3751)
                     /locus_tag="VGR22_06235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEV2128197.1"
                     /translation="MSPELLERYHEAIDRLDALIASTPSRTDTSREAVRRRAAERMGR
                     LRNFLAFLGDPAAAIPKIHIGGTSGKGSTTTALAAILSAAGYRTGAHTSPYLQVPSEK
                     LHFDGDLIDAERFVRLVDHLLAALAEFPDADHITYGEAWIALVMLFLEDIGAEVGIIE
                     VGAGGRFDLSNIVDPELAIITSVGIDHTNTLGDTIEQIAWHKAGIIKPGRLALSTVDD
                     PRAQPFMREEAAAVGACLREVDMATAIRDIQLSDIETSWVEAATGQRWTIGMAGRFQA
                     RNGQAAREAARMLAQRGWRIADAAIDRGLRAARIPGRAERMPGEPRTMLDGAHNAQKV
                     TALAADLPELLPVPEGARRIIVLGALEAKQVREALATLLPHADMLIATSPRVFGKEAR
                     AADDLAALAREVGFGDRALPIADPREALERARTVSKPGRADAILVTGSLYLVGNIRDL
                     WYPADRIAMAGTPWPIDGAPGDMATTHAGARP"
     gene            3902..4606
                     /locus_tag="VGR22_06240"
     CDS             3902..4606
                     /locus_tag="VGR22_06240"
                     /inference="COORDINATES: protein motif:HMM:NF041646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VC0807 family protein"
                     /protein_id="HEV2128198.1"
                     /translation="MLNILITLIIPVVILMRFSGEDELGPARGLVLALAFPIGYAIYE
                     IWKQRKVSWTPIVGVVSVLLTGGIALLELPPDLIAIKEASIPLILALAILFSAWMGKP
                     LARVFLSQMLDREKVEAALRERGTYDEYEQRTARATYMLASAFLLSAALNYGLAKVVV
                     TSDPGTEAFNSELGRMTALSFPVITIPVFVVLFITIFYVLRIVNQLTGLEAEEVVRQK
                     GKESAPTTTQNRTQET"
BASE COUNT          789 a         1429 c         1659 g          858 t
ORIGIN      
        1 ggatgccttc gtcccgcagg atgcccgcga tcaggttggc ctcgatctca tttggtgctg
       61 tcgtcagtag caccggtgag gcgttggtgg cagaggcttc gagatcgtcg gggttgggtg
      121 gggtgtcgac gagcctgctg ccgcactccg ggcaggacgc gacccagtcg tcgaaagcac
      181 catcgccgag gggacagtag cgcgtcatgg ctgcgacctt tctgctgcat cgagacaagt
      241 ccacccgggt ggacgccgag tgcccgccat gggattcggt ctgcctctcg acgcctgttc
      301 gggaatccag taggcttgca tcaaccgtgg atgcatggca tcacgatagc gtagtctgtg
      361 catcgaagtg agtgtccgac acaacgatgg gtgtcggtga acccaggcat gccgacagga
      421 caggacggag cgggagatgc aggagatcag tcggatcatc acggccctcg gcgcggtcgt
      481 ggccagcact ggtctggtgg tgtatggcat gggggggtcc tactggaccc caggcgattt
      541 cgagctgaat cttggtgtgt ggatgatggt cctcggcgtg atcgccacga tcgtcggtat
      601 catcctctac cagcgaacct gggtcgagca cgactagtca cttctttgca tcgatgcaca
      661 tgccgtcagg atgcccgcga ccccgaatgg ggcgacgggc atcattcgtt ctcgccctcg
      721 gccaaccggg catcgttggc ggcgatcgcc gtggcaacgg cgccggcaag gtgatggagc
      781 agcgagagct ccgcagggct gaaggtgtga aggtgccgct gcgaggcaac catcgctccg
      841 cgcggccgtg cgttcacgtg taccggcagg atcacggtgg agcgcagacg ctcccgggtg
      901 tggagcggca tgatctccgg cgtcatgtcg ggctccttgt ggacatcctc aagcaggacc
      961 tcgaagctgt gctgcaggaa atcgcgcagg atgcgcggcg gcaccggcac ccgcgtgcca
     1021 agaaacgacg gatccatggc accggtggcg gcgtgccaga aaacgtggtc attgaccgtt
     1081 tcggcgatgg ccacgaagtc gagattggtc acaagctgcg acgtccggca gatcacctcg
     1141 aacgcgtcct gcaggcgcgg agtgcgcgcg acggcgttca tcggccggca ggtgatgtcc
     1201 atctcgacta cctgctgctc cagcgattcc atccgcgcca gccgttgcat tagcgctccc
     1261 gctggccgta ccagccggtc gatcgactcg gcatggcgag cggctgccac cggtgagacg
     1321 gtctccggcc aggcagcgat catcgcccac atgctcgtct cgtggccatc gagcaccgag
     1381 atcgcggcag cgttgatggc gagtaggtca tggcacgcgt ccatggtgcg caccggcaca
     1441 ccagcgtcac cgattgcacg gggatcggtg aacagggttg tggcacagcc gggtgacagg
     1501 cccccggcca gcagctctgc gacccgtgat gtgatctgtt ccccgacgtc gtcgagggat
     1561 tggcccatca gcaggtccgg ctgtgggttc cgcgcctcca cgaagcgcac gttcctgtgg
     1621 tcgagttgga acaggagtac ggtggcgtcg gtgatcgcga tgaccacgcg ttgcagctcg
     1681 tccagtaact gagtggtcga ggacgtgtgc atgagccggt cgatcgttct ggagatctcc
     1741 cgggacgttt ccttgtgccc ggtggtctcg ctgatgtcgt ggctggcaag tgcgagaccg
     1801 agcacgcggg cgaaggtttc gacgacgcgc gcttcggctg tagagaaggg tgcgggcgat
     1861 gtgcaggaga gtacgagcac cgcggcggtg tcggcatcga cgaaaaccgg cgcggccaac
     1921 gcttggcgca gcgctgcctg tcgtcgcggc ccgtcctgcc gatcgacgtc gtcgtgatcc
     1981 ggaatcgtgt ggtacacggg gcggttttcc tgcacggctt gatgcaccag tgccgggaag
     2041 gtggcaggca cgctgtggtg ctgtgctgcc gtgctgagcg aaatgcctcg ctcgtaaatg
     2101 acggtggcac ggtccgttcg cctgtccaga cagctgatgg agaggcgatc gatggtgacg
     2161 agccgagtga gtgcctggtc gaacgcgtcg aagggttcgc ggctgtcgtc ggcagcctcg
     2221 gtagcacgga ggaaatccag caggacatcg ccgttgagtt cgtcacggcc gaataggtcg
     2281 gaatgccata cagaatcgac cgtcatggac gcgctcctgc atgtgtggtc gccatgtcgc
     2341 ctggtgctcc atcgatgggc cacggtgtgc ccgccatggc gatgcgatcg gccgggtacc
     2401 agagatcccg gatgttgccg acgagataga gcgagcctgt caccagaata gcatcggcgc
     2461 ggcccggttt gctcaccgtt ctggcccgtt cgagtgcttc gcgggggtcg gcgatcggca
     2521 gagcacggtc gccgaagccc acctcgcgcg ccagggcggc gaggtcgtcg gcggctcggg
     2581 cctccttgcc gaagacccgt ggcgaggtcg cgatcagcat gtcggcgtgg gggaggagag
     2641 tggcgagggc ctcccgcacc tgcttcgcct cgagggcgcc cagcacaatg atgcgtctgg
     2701 cgccctccgg caccggcaac agctccggta ggtcggctgc gagcgccgtg accttctgtg
     2761 cgttgtgcgc accgtccagc atggtgcgtg gctcgcccgg cattctctcc gcgcgcccgg
     2821 ggatccgtgc cgcgcgcagc ccgcggtcga ttgcagcatc ggcgatgcgc cagccccgtt
     2881 gggccagcat tcgtgccgcc tcccgggccg cctgcccgtt gcgtgcctgg aagcgtccgg
     2941 ccatgccgat cgtccatcgc tggccggtgg ccgcctcgac ccagctcgtc tcgatgtcgg
     3001 agagctggat atcgcgaatc gcggtggcca tgtcgacctc gcgaaggcag gcgccgacag
     3061 cagcagcttc ctcccgcatg aagggctggg cgcgcgggtc atcgacggtg ctgagtgcga
     3121 gacggcccgg tttgatgatg cccgccttgt gccacgcgat ctgttcgatg gtatcaccga
     3181 gggtgttggt atggtcgatg ccgaccgagg tgatgatcgc cagttcgggg tcgacgatgt
     3241 tcgagagatc gaagcgaccg ccggccccga cctcgatgat gccaacctcg gcaccgatat
     3301 cctccaggaa gagcatcacc agcgcgatcc acgcctcacc gtaggtgatg tggtcggcat
     3361 ccgggaactc ggcgagcgcg gcgagcaggt ggtcgaccag ccggacgaac cgctcggcat
     3421 cgatcaggtc accatcgaag tgcagcttct cactgggcac ttggagatag ggggaggtgt
     3481 gggccccggt gcggtagccg gcagcggaga ggatcgccgc gagcgccgtg gtcgtggagc
     3541 ccttgccgga ggtgccgccg atgtggatct tcgggatcgc ggcagcgggg tcgcccagaa
     3601 aggcaaggaa gttccggagc cgtcccatgc gctctgcggc acgacggcga accgcctcac
     3661 gagaggtgtc ggtgcgactc ggcgtgctgg cgatgagcgc gtcgaggcgg tcgatcgcct
     3721 cgtggtaccg ttcgagcagt tcaggcgaca tgtgggctcc aggagtgttg cggctgcaag
     3781 gcaaccgcga cgcaagcggt accattatga ttatgatggt ttcacggagc gggtgatgaa
     3841 gcggcaggca cgctggcgcc ccgttcgccg taagtgacgc gacgcagagg acagaggctg
     3901 catgctcaac atcctgatca ccctcatcat cccggtcgtc atcctgatgc gcttcagcgg
     3961 cgaggatgag ctgggccccg cccgcgggct ggtcctcgcg ctggcgttcc cgatcggcta
     4021 cgccatctac gagatctgga agcagcggaa ggtcagctgg accccgatcg tcggtgttgt
     4081 cagcgtgttg ctgaccggtg gcatcgcgct gttggagctt ccgccggatt tgatcgcgat
     4141 caaggaggcg tcgatcccgc tgatcctcgc gctggcgatc ctgttctcgg catggatggg
     4201 caagccgctg gcacgtgtct ttctcagcca gatgctggac cgcgagaagg tggaagccgc
     4261 cctgcgcgag cggggcacct acgacgagta cgagcagcgc accgcgcgcg cgacgtacat
     4321 gctggcgagc gcgttcctgc tctcggcggc gctcaactac ggtcttgcga aggtggtggt
     4381 caccagcgac ccgggcacgg aggcattcaa cagcgagctg gggcggatga ccgcgttgag
     4441 cttcccggtg attacgattc cggtcttcgt cgttctcttc atcacgatct tctacgtcct
     4501 gcgaatcgtg aaccagctga ccggactgga ggcggaggag gtcgtgcgcc agaaggggaa
     4561 ggagtcggcc ccgaccacga cacagaaccg cacccaggaa acctgattgc cagcttcgga
     4621 gccaactgat cgtccgtatc ctgtagcgcc gcctcttgtg ggcggcgctt gtggttcagg
     4681 ccacgaactg aggaagagac gattgatgcc tgtagaccca tgaaccggtg gaagc
//
LOCUS       DASYWL010000175         3399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_477434, whole genome shotgun sequence.
ACCESSION   DASYWL010000175 DASYWL010000000
VERSION     DASYWL010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477434"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="VGR22_06245"
     CDS             complement(<1..825)
                     /locus_tag="VGR22_06245"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEV2128199.1"
                     /translation="MPSPNRKVIISCAITGGIHVPSQTPHLPITPDQIADSAVGAAEA
                     GAAILHLHARDPQTGKPTPDPDVWEQFLPKIHGRTDAVINMTTGGGLTMSVEERIRGP
                     LRFKPEMCSLNMGSINFGLYPMKNRIREYKHDWEEPYLEGTRGGIFRNTFLDIETFLT
                     ELGKAHGTRFEYECYDIGHVYNLAHFLDRGMIKPPIFVQSIFGILGGIGTDPEDLLHM
                     RRTLDRLIGRDNYVWSILGAGRHQTNLVTMGAIMGGNTRVGLEDNIYLEKGKLAETN"
     gene            complement(911..1774)
                     /locus_tag="VGR22_06250"
     CDS             complement(911..1774)
                     /locus_tag="VGR22_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128200.1"
                     /translation="MGTNAPELRYPSLPVVVTTVIDALAAHPAVTAITLGGSRATARD
                     DQASDQDIEVFVDGEVPSALRRELALRFDPDPEIDNSQFGPTDEWSDRASGVAIDIAY
                     FERDWFEGTIRDVIERHHPELGYTTAFWHTLRHARPMFDRAGWLAEMQTLAATPYPEA
                     LRRNVVAWNHPVLRDVRSSWRHQIELAVMRDDPVSVNHRVAALLASVFDIVFALTRTL
                     HPGEKRLLTHVARLGDAVPPGFDAQVRALLCAAGNPEGNDMLAAVDAVCNTVDAMIEQ
                     HGLRVAATRVG"
     gene            complement(2290..2952)
                     /locus_tag="VGR22_06255"
     CDS             complement(2290..2952)
                     /locus_tag="VGR22_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2128201.1"
                     /translation="MLPEASTATAALTTGEVDFTTVPSGEAEPLRDDPGIEIHAFDTA
                     DFNLFGVNQDSERTELFTDVRVRQALMSALDRDLIAEEICLGFATRADGTQPPLSIAY
                     APDSVATIYDYDPDRACHLLDEAGWVEGEDGIREKDGVPFRFECLYGAGIAIYEQQLP
                     YMQQAWREVGLEMQPVSVPLQTLATPATTKCVSMASHGASMESRAAWFAAMQCRPPAS
                     MR"
     gene            complement(2876..>3399)
                     /locus_tag="VGR22_06260"
     CDS             complement(2876..>3399)
                     /locus_tag="VGR22_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2128202.1"
                     /translation="VTYTFHLNPNARWHDGEPLTAEDVVFSFDAILDETSLSWSYWAV
                     DLALESYRAIDDHTVELVARDRLAVFVENTARLVPIVVKHVWEGIAPGDWGTDPGAIG
                     TDPDRVIGSGPFRFVEWIAGDHVTLVGNDDYLEPKWYPRHRRVHLPGAARGVNRHRGA
                     HHRRGRLHHRAVR"
BASE COUNT          573 a         1043 c         1147 g          636 t
ORIGIN      
        1 gttcgtctca gccagcttcc ccttctccag gtagatgttg tcctcgagtc ccacgcgggt
       61 gttgccgccc atgatcgcgc ccatcgtcac caggttcgtc tggtgccgac cggcaccgag
      121 gatcgaccag acgtagttgt ctcgcccgat gaggcggtcg agcgtccgcc gcatgtgcag
      181 cagatcctcc ggatcggtgc cgatgccgcc aaggattccg aagatcgact gcacgaagat
      241 gggaggcttg atcatgccgc ggtcgaggaa gtgtgccagg ttgtagacat gcccgatgtc
      301 gtagcactcg tattcgaagc gggtgccgtg cgccttgccg agctccgtga ggaaggtttc
      361 gatatcgagg aaggtgttgc ggaagatgcc accgcgcgtg ccctccagat acggctcctc
      421 ccagtcgtgc ttgtactccc tgatgcggtt cttcattgga tagagcccga agttgatcga
      481 gcccatgttg agcgagcaca tctccggctt gaagcgcagc ggaccgcgga tccgctcctc
      541 gacggacatc gtcaacccgc cgccggtcgt catgttgatc accgcgtcgg tgcggccgtg
      601 gattttcggc aggaactgtt cccagacgtc cggatcgggc gtcggcttgc cggtttgcgg
      661 atcgcgtgcg tggaggtgca ggatcgccgc gccagcctcg gcagccccga cagcggagtc
      721 ggcgatctgg tcgggcgtga tcggcaggtg aggggtctgg gacggaacgt ggattccgcc
      781 agtgatggcg caggagatga tgaccttgcg gttgggggac ggcatcggtg gctccttcac
      841 gggtgaccgg attgctttgg tccccagatg gtagccgatg ccctcggttc gggcgagagg
      901 gatggtcgcg tcaacccacc cgagtggctg cgacccgaag accgtgctgc tcgatcatcg
      961 cgtcgaccgt gttgcagacg gcatcgacgg cggccagcat atcgtttcct tcggggttac
     1021 ccgctgcaca aagcagtgca cgcacctgag cgtcgaaccc cggtggcacc gcatcgccaa
     1081 ggcgtgcgac atgagtcagc aggcgcttct cgccggggtg gagggtgcgg gtgagcgcga
     1141 agacgatgtc gaacacactc gcaagcagcg ccgccacgcg gtggttgacg ctgacgggat
     1201 cgtcccgcat gaccgcgagc tcgatctggt gccgccagga cgaccgcacg tcacgcagca
     1261 cgggatgatt ccacgcgacg acgttgcgcc gcagcgcttc ggggtacgga gttgcggcga
     1321 gggtctgcat ctccgccaac cagccggcgc gatcgaacat cggccgggcg tgtcgcagag
     1381 tgtgccagaa cgcggtggtg tagccgagct cgggatggtg gcgctcgatg acgtcgcgga
     1441 tcgtgccctc gaaccagtcg cgctcgaagt aggcgatgtc gatcgcgact ccgctggcgc
     1501 ggtccgacca ctcgtcggtt gggccgaact gggaattgtc gatctccggg tccggatcga
     1561 accgcagcgc gagttcccgg cgcagagcgg aaggaacctc gccgtcgacg aagacctcga
     1621 tatcctgatc ggacgcctga tcatcgcgtg ccgtcgcccg cgacccaccg agcgtgatcg
     1681 cggtcaccgc gggatgggcc gcgagagcgt cgatgaccgt cgtgaccaca accggcaaag
     1741 atgggtatcg aagttcaggc gcgttcgtgc ccatgaagtt catccctgat gggcacaccg
     1801 atgccccaga ttcccgagaa tgctgacgga tggtctcgtc gtttgccgtg gagtgcgcgc
     1861 ccccccagcg gcatccgtcc cacgaggatg acgtcgagct cccgcagcga agccgagccg
     1921 aagcatacgc gaaatggagg cttgcaggat gttgcagctt cgaggctccg gccttcggcg
     1981 ctcacgggtt tcttctggtg ttcgtcgatg ctttggcgcc tgcatctccc tctgttccgc
     2041 atcgggctgc gctactgctg cacctctgtc caccaccacg gcatgctcca gaggaagctg
     2101 tagccgttcg gtaggaagtt gtgcagcgtg cggcgagagc cgaccaccgc ctgccggaag
     2161 agcagaatgc cggctgcgac atcgtcgttg acgatgttcg actgctcgat gagcaggtcg
     2221 attcgctgtt cgacgtcgag ttcccgcttc tgcagcgcat cgagtcatcg tatcgctcgt
     2281 tgcagtactt catcgcattg aagccggcgg gcggcactgc atcgcagcga accatgccgc
     2341 cctgctctcc atcgacgctc catgtgaagc catagacacg catttcgtag tcgccggtgt
     2401 cgccagggtc tgcaggggta cggacactgg ttgcatctcc aggccgacct cacgccatgc
     2461 ctgctgcatg tacggcagtt gttgctcgta aatggcgatc cccgccccgt acaagcactc
     2521 gaacctgaac ggtacgccat ccttctcgcg gatgccgtct tcgccctcaa cccagccggc
     2581 ttcgtcgaga aggtgacacg ctcgatcggg atcgtagtcg tagatagtgg cgacgctgtc
     2641 gggcgcgtag gcgatcgaga gcggtggctg ggtgccatcc gccctcgttg cgaacccgag
     2701 gcagatctcc tcggcgatca ggtcgcggtc gagcgcggac atcagcgcct ggcgcacgcg
     2761 cacgtctgta aagagctcgg tgcgctccga atcctggttc acgccgaaga ggttgaagtc
     2821 ggcggtgtcg aaggcgtgga tttcgatgcc tggatcgtcg cggaggggct cggcctcacc
     2881 ggacggcacg gtggtgaagt cgacctcgcc ggtggtgagc gccgcggtgg cggttgacgc
     2941 ctcgggcagc acccggtaga tgaactcgtc gatgacgggg ataccatttg ggttccaaat
     3001 agtcgtcgtt gccgacaagg gtgacgtgat cgcctgcgat ccactcgacg aaccggaagg
     3061 ggccggaacc gattacgcgg tccgggtcgg tgccgatcgc gccggggtcc gtgccccagt
     3121 cgccaggcgc gatcccctcc cagacgtgct tcacgacgat ggggacgagc cgggcggtgt
     3181 tctcgacgaa gacggcgaga cggtcgcggg caaccagctc aaccgtgtgg tcgtcgatcg
     3241 cgcggtagct ctccagtgcg aggtcgacag cccaatagct ccagctgaga ctggtttcgt
     3301 cgaggatcgc gtcgaaggag aagacgacat cctcggccgt gaggggctcg ccgtcgtgcc
     3361 agcgcgcgtt cgggttcagg tggaaggtgt aggtgactc
//
LOCUS       DASYWL010000176         6218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_196040, whole genome shotgun sequence.
ACCESSION   DASYWL010000176 DASYWL010000000
VERSION     DASYWL010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6218
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196040"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..2433)
                     /locus_tag="VGR22_06265"
     CDS             complement(49..2433)
                     /locus_tag="VGR22_06265"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2128203.1"
                     /translation="MSTSHTGAPPPPEYQAAARHTVRDPLTRIVGRDGLIASLEALLD
                     RGDTRLVTLTGPGGIGKTRLSLEVASRVQRDFAHGACFVSLDSVRDPEAVPAAVAEAL
                     GLRERGGQPLPEAITTTLRAQHLLLVLDNFEQVTPASLWVAELLTACPRVKALVTSRA
                     RLRIQGERHMSVPPLPVANGDLRRLDDVLEHSPAMALFVERARALRPDFALTDANAVA
                     IRDICQQLDGLPLAIELAAAKVTVLSPYAILARLSDRLALLTGNLRDAPPRLQTMRNA
                     IAWSYDLLQPEEKQLFRRLSIFAGTFSLADAVAVGLEPEVARGEPRGGMPDLAELQEV
                     ASLLDQSLIQYVSSDHGESRFRMLTMIREFGLEQLKASSEADRIARRHARHVLALVER
                     SGSELTGPDQVRWLNHLASIHDDIRAAVAWLQQQGEHELQLRLAVALWPYAYIRGYLT
                     EMRKWLDAAIETNPEPMAMRAEALNASGVLASVQGDILTAVARHNAALELSEMLGDQR
                     GLAMALNGLGNAAEVEGDHALAFERYGEARDRFREIGDRRSVGVMLINLGNLYWMDGD
                     LDKAIAYQQEAGDLFREVGSQRGVAWSATNLGSIAFEQGDLTGATKRLLEAIGLYREL
                     GDQSGIIETLERFADIAGARGQRERQATLLDAAAGIRQAINIPVQAINRERYDATIAD
                     LREHLGETYDSIWKAGQKLSIDEVLAIVSASESFEQPVATLPRDAAAEAHGLTQRELE
                     VLRLMADGMTNQEIADTLYLSPRTVTSHASNILGKLGTNTRTAAVAYAIRNDLA"
     gene            complement(2817..3203)
                     /locus_tag="VGR22_06270"
     CDS             complement(2817..3203)
                     /locus_tag="VGR22_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128204.1"
                     /translation="MIPATVGAFILDRVKQEIFSTPEEAEQAVEAIEESIIANDDHIG
                     TVYTDADGNEIGQAVRMLNVAIYVVSNERGDPEKDLLAAMRDPESEVIFHTDGGRVSR
                     VIYIPPAKPDYKQTGPVIKGFRADDI"
     gene            complement(3221..4111)
                     /locus_tag="VGR22_06275"
     CDS             complement(3221..4111)
                     /locus_tag="VGR22_06275"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEV2128205.1"
                     /translation="MDGTALLAASFRRELQVPVRRVASLQHQLEDSMTDYEPRDMEQA
                     PPSPAEIPPTDDTPAPRRMLLIQAHPDDAEFMCAGTVAEWVQDGAEVHYCSITSGDKG
                     TSDLGVSSSELATLRECEQRAACEVLGVTSVIFLGYQDATLVPDLALRRELTRVIRRV
                     KPDVLMCQDPTMRYSGQRYINHPDHIAAGEASLAAVFPSARDHKTFPELLDEGLEAHI
                     TPEVYIYGANQADVWVDISSSIDTKIAALKAHESQVGDRADELDEMIREWGRETARLH
                     PHKPEDFGEYAESFKYMKLG"
     gene            4177..4722
                     /locus_tag="VGR22_06280"
     CDS             4177..4722
                     /locus_tag="VGR22_06280"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEV2128206.1"
                     /translation="MSAVSSEQPTPPGARVIEVPERLPEPRHQLDPRARSLWRIGALF
                     NGALLVFFVTLMAFAIRRVIVDYDARWAWLVTGIVLALALLNAWLGPNLRYRYWRYEI
                     RADEADLQHGWLVRTRQLVPMGRIQHVDTRRGPLDRRFGLATVVLYTAAGPAFIPALS
                     SEVAADVRDRIATLANVRDDI"
     gene            4741..>6218
                     /locus_tag="VGR22_06285"
     CDS             4741..>6218
                     /locus_tag="VGR22_06285"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEV2128207.1"
                     /translation="MTDPATDPSVAARDEHASETPVTASEDITWHRMSSRFIVVGTLR
                     QLRGLVVPAALILLTRNMGEGRSNLIYIGIALIVTVFAALLSLVDWWNFRYALADHEL
                     LVRSGVVQKQERVVPYGRIQAINLDEAPLERLVGIVRVKVETAAGGTADIEIKGIDRS
                     AAPRLREALATARVQARQQGDAEVPPIAPAEETRNVPKAVDVGAGGELLRRLSTPELF
                     ALGATSGRIGPMAAVIGAILQFGTDIMPQTWWNRIPWQEAEALRSVSVATALLVVIAV
                     VAWVMAIASTALTFGGFELHRNGDQLIVQHGLLDRRRRTIPIQRIQAVIVGEQLLRQP
                     FHHADLRFESAGGEVEGASGDSGVLFPYLPLHEVEPLLRRAAPEFATDPTVPMAARLP
                     IRSLRRYIFSATVGWVVFVGAAAAALGFWLDTWIDVVAWRQVLLLLVVAPVFAGLGWA
                     RWRDGGWEVDGSNLLLRWRTVSRQTFITNTSRIQRRRITTDP"
BASE COUNT          961 a         2129 c         2031 g         1097 t
ORIGIN      
        1 ggcgtctatc gcgtcgccca ccgagggaca ctgccgcaga cgtcttcgtc acgcgagatc
       61 gttgcggatg gcgtaggcga cggcggcggt gcgagtgttc gtgccgagct tgccgaggat
      121 gttgctggca tgactggtca ccgtgcgcgg gctgaggtag agcgtatcgg cgatctcctg
      181 gttggtcatg ccatccgcca tcaaccgcag cacctccaac tcccgctggg tgagcccgtg
      241 cgcctcagct gcggcgtctc tcggtagtgt tgccaccggc tgctcgaaag actcggatgc
      301 actgacgatc gccagtacct cgtcgatgct cagcttctgt cccgccttcc agatggaatc
      361 gtaggtctcc ccgaggtgtt cgcgcaggtc ggcgatcgtc gcgtcgtagc gctcgcggtt
      421 gatggcctgt acggggatat tgatggcttg gcgtatcccg gcggcggcgt ccagcagtgt
      481 ggcctgccgc tcgcgctggc ccctggctcc tgcaatgtcg gcgaagcgct cgagcgtttc
      541 gatgatgccg gactggtcgc ccagctcccg ataaagcccg attgcctcga ggagtcgctt
      601 ggtagcgcca gtcagatcgc cctgttcgaa ggcaatcgag ccgaggttgg tggccgacca
      661 ggcgacgcct cgctgactac cgacctcccg gaacaggtcc cctgcctcct gctggtaggc
      721 aatcgcttta tccaggtccc cgtccatcca gtacaggttt ccaagattga tcagcatcac
      781 gccgacgcta cgccggtcgc cgatctcacg aaagcggtct cgcgcctcgc cgtatcgctc
      841 gaacgcgagg gcgtgatctc cctcgacctc tgccgcattg ccaagcccgt tgagggccat
      901 cgcgaggcca cgctggtcac ccagcatctc gctcaactca agggcagcgt tgtgacgcgc
      961 gaccgcggtg aggatatccc cctgcacact ggcaagcaca cccgaggcgt tgagcgcctc
     1021 ggcccgcatg gccatcggct ccgggttcgt ctcgattgcg gcatcgagcc acttccgcat
     1081 ctctgtgagg tagccacgga tgtaggcgta tggccagagc gcgaccgcca gccgcagctg
     1141 cagctcgtgc tcaccctgct gctggagcca cgcgacggcg gcacggatat catcatggat
     1201 ggacgcgagg tgattcagcc accgcacctg gtctggaccg gtcagctccg agccggatcg
     1261 ctcgacgagc gccagcacgt gccgcgcatg ccgtcgcgcg atccgatcgg cctcgctgct
     1321 cgccttgagc tgctccagtc cgaactcccg gatcatcgtg agcatgcgga agcgtgactc
     1381 gccatggtcc gaggacacgt actggatcag gctctgatcg agcagcgagg caacctcctg
     1441 aagctccgca agatcaggca tgccgccgcg gggttccccg cgcgccacct cgggctccaa
     1501 cccgaccgcc acggcatcgg caagcgaaaa ggtcccggcg aagatggaaa gccgccgaaa
     1561 gagctgcttc tcctccggct ggagcaggtc gtagctccag gcaatggcgt tgcgcatggt
     1621 ctgcagccgg ggcggcgcat cgcgcaggtt gccggtcagc agggccagcc ggtcggacag
     1681 tcgcgccagg atcgcgtagg gcgacagcac ggtgaccttg gcggccgcca gctcgatcgc
     1741 cagtggcaat ccgtcaagct gctggcagat gtcccggatg gccacggcgt tcgcgtcggt
     1801 gagggcaaag tccggccgga gggcgcgagc ccgctcgacg aagagtgcca tggccgggga
     1861 atgctccagc acgtcgtcca accgccggag atcgccattg gccacgggca acggcggcac
     1921 cgacatgtgc cgctcgccct gaatgcgcaa gcgcgcgcgg gaggtgacca gcgccttgac
     1981 ccggggacag gcggtgagca gctcggccac ccacagcgac gcaggggtca cctgctcgaa
     2041 gttgtcgagc accagcagca gatgctgcgc gcgcagcgta gtcgtgatcg cctcggggag
     2101 cggctgaccg ccgcgctcgc ggaggccgag cgcctcggca accgccgccg gcactgcctc
     2161 ggggtctcgg accgagtcta gggagacaaa gcaggcgccg tgcgcgaagt cgcgctgcac
     2221 tcgcgatgct acctcaaggg agaggcgtgt cttgccgatc ccgccagggc cggtcagggt
     2281 caccagccgg gtatcaccgc gatcgagcag tgcttcgagg ctggcgatca gcccgtcgcg
     2341 gccgacgatg cgggtgagcg gatcacgtac ggtgtgccgt gcggcagctt ggtactctgg
     2401 cggcggtggt gcgcccgtat gcgaggttga cattggttcg gacgacggtc ctcccatcat
     2461 gtcgcgcccc tctcggtgaa cctgatcgta gcgtgataac cgttacatcg tcggactccg
     2521 ccggcatttt gtccacaact ttccatcagc cactggtcat gccgtgccag caagccctgt
     2581 gcctcgaaac gcggcacgtc gtctcatatc tgcgacagcc ctgcacatcg tatgacaagc
     2641 cccgcgcctg cggcgtgggg ctgacagata cacatgctcg tgcgggcggc caatcgggac
     2701 tgctggatac cgctgatcgg cggtatccag cacgcaacgc agcgcgtccg gagcccggcc
     2761 acatccgcct cgggagcgtc atgacaccgg gccgtcgcca cgtcatgctg ctagatctag
     2821 atgtcgtcgg cgcggaagcc cttgatgacc ggtcccgtct gcttgtagtc cggcttggca
     2881 ggcgggatgt agatcacccg cgagactcgg cccccgtccg tgtggaagat gacctcgctc
     2941 tcggggtcgc gcatcgccgc gagcaggtcc ttctcggggt ctccccgctc attcgagacg
     3001 acgtagatgg cgacgttgag cattcgcacc gcctgcccaa tctcgttgcc gtcggcgtcg
     3061 gtgtagaccg tgccgatgtg gtcgtcgttg gcgatgatcg actcctcgat cgcctcgacg
     3121 gcctgctcgg cctcctccgg cgtcgagaaa atctcctgct tgacccgatc gagaatgaag
     3181 gcacctacgg ttgcaggaat cacaatggtc ctcctgtgcg ctagccgagc ttcatgtact
     3241 tgaagctctc ggcgtactcg ccgaagtcct caggcttgtg gggatgcagc ctggcggtct
     3301 cccggcccca ctcgcggatc atctcgtcca gttcgtccgc gcggtcgccg acctggctct
     3361 cgtgcgcctt gagcgccgcg attttggtat cgatggatga cgagatatcg acccacacgt
     3421 ccgcctggtt cgccccataa atgtacacct cgggtgtgat gtgcgcctcc aacccttcgt
     3481 ccagcagctc gggaaaggtc ttgtgatcgc gcgccgaggg gaagaccgcc gccagcgatg
     3541 cctcgcccgc tgcgatgtgg tccggatggt tgatgtagcg ctggccggag tagcgcatgg
     3601 tcgggtcctg gcacatcagc acgtcgggct tgacgcggcg gatcacgcga gtcagttccc
     3661 ggcgcagcgc gaggtctggc acgagggtgg cgtcctggta gccgaggaag atgacgcttg
     3721 tcacgccaag gacctcacag gcggcgcgct gctcgcactc gcgcaacgtt gccaactcgc
     3781 tgctggagac gcccagatcg gaggtcccct tgtcgccgct ggtgatcgag cagtagtgca
     3841 cctccgcgcc gtcctgcacc cattcggcaa ccgtaccggc gcacatgaac tcggcgtcgt
     3901 ccggatgcgc ttggatcagc agcatgcgcc ggggagctgg ggtatcgtcc gttggcggga
     3961 tctccgccgg tgacggtggt gcctgctcca tgtctcgggg ctcgtagtcg gtcatactat
     4021 cctcaagctg gtgctgcaga gacgcaaccc gtcgcaccgg cacctgcagc tcgcgccgga
     4081 acgacgcggc cagcagggcc gtgccgtcca cacgcccttc atcatacttg gggacagcgc
     4141 ggctgtgtcc acccggccgt tgcatggaga acgcgcatgt ccgccgtatc gagcgagcaa
     4201 cccaccccgc cgggcgcccg cgtgatcgag gtgccggagc gcctgcccga gccgcgacac
     4261 cagctcgacc cacgcgctag gtcgctctgg cgtatcggcg cactcttcaa cggcgcgctg
     4321 ctggtgttct tcgtgacctt gatggcgttc gccatccggc gggtgatcgt cgactacgac
     4381 gcgcgctggg cgtggctggt caccggcatc gtactcgctc tggcgctgct caacgcctgg
     4441 ctcggcccga acctgcgcta ccgctactgg cgttacgaga ttcgcgctga cgaggcggac
     4501 ctgcagcacg gctggctggt gcgcacacgg cagctcgtgc cgatgggacg gatccagcac
     4561 gtcgatacgc gccgaggacc cctcgaccgc cgcttcgggc tcgccacggt cgtgctctac
     4621 acagccgctg gcccggcctt catcccggcg ctgtcgagcg aggtcgccgc cgacgtccgc
     4681 gaccggatcg ccactctcgc caacgtccgc gacgatatct gacctgcgcc gaggaggctc
     4741 atgaccgacc ccgccaccga cccgtccgta gcggcacgtg atgagcatgc gtcggagacg
     4801 cccgtcaccg ccagcgagga catcacttgg caccgcatgt catcccgctt catcgtggtc
     4861 ggcacactgc gtcagctgcg cggcctggtg gtgcccgccg ccctgatcct gctcacccgc
     4921 aacatgggcg aggggcgctc caacctgatc tacatcggca tcgcattgat cgtcaccgtc
     4981 ttcgcggccc tgctcagcct cgttgactgg tggaactttc gctatgccct cgccgaccat
     5041 gagctgctgg tgcgctccgg cgtcgtccag aagcaggagc gtgtcgtccc ctatggccgc
     5101 atccaggcga tcaaccttga tgaggccccg ctggagcgcc tcgtcggcat cgtcagggtg
     5161 aaggtggaaa cggcagcggg cggcaccgct gacatcgaga tcaagggcat cgatcggtcc
     5221 gccgcgccac gcctgcgaga ggcactcgcg acggcccgcg tccaagcccg ccagcagggc
     5281 gatgccgagg tgccgccgat cgcccccgcc gaggaaaccc ggaatgtacc caaggcggtt
     5341 gatgtcggtg ctggcggcga gctgctccgc agactttcga cacccgagct gttcgcgctg
     5401 ggggcgacct ccggtcgcat cggcccgatg gcggcggtga tcggcgcgat cctccagttc
     5461 ggcaccgaca tcatgccgca gacgtggtgg aaccgcattc cgtggcagga agcggaggca
     5521 ctgcgcagcg tcagcgtcgc gaccgccctg ctcgtggtca tcgctgtggt cgcatgggta
     5581 atggccatcg catcgaccgc gctcaccttc ggcgggttcg agctgcaccg caacggcgac
     5641 caactcattg tgcagcacgg gctgctcgac cgccgtcgtc gcacgatccc catccagcgc
     5701 attcaggccg tcatcgtcgg cgagcaactg ctccggcaac cgttccacca cgccgacctt
     5761 cgcttcgaga gcgccggagg cgaggtggaa ggcgccagcg gtgactcggg cgtgctcttc
     5821 ccctacctgc cgctgcacga ggtggagcct ctgctccgcc gcgctgcacc ggagttcgcg
     5881 actgatccga ccgtgccgat ggcggcccgg ctgccaatcc gctcgctgcg ccgctacatc
     5941 ttctcggcga cggttgggtg ggtggtcttc gtcggtgccg cggcggccgc gctcggcttc
     6001 tggctggaca cctggatcga tgtcgtcgcc tggcgccagg tgctgctcct gctggtggtc
     6061 gcgccggtct tcgccgggct tggctgggcc cgctggcggg acggcggctg ggaggtggac
     6121 ggctccaatc tgctgctccg gtggcgcacc gtctcccggc agacatttat cacgaacacg
     6181 tctcgcatcc agcgccgccg catcaccacc gacccctt
//
LOCUS       DASYWL010000177         3005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_177223, whole genome shotgun sequence.
ACCESSION   DASYWL010000177 DASYWL010000000
VERSION     DASYWL010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177223"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VGR22_06290"
     CDS             complement(<1..356)
                     /locus_tag="VGR22_06290"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV2128208.1"
                     /translation="MPGPSHQCNSATTRLAQFVEDDVRCWPRRSTRRYPKAQARCSGS
                     GSAPARMLVMRAMVFIMDQSHTTKLRIAELAAQLNLNPKTIRYYEQIGLVPAPARTPA
                     GYRLYGLAEQDRLRFIV"
     gene            741..1613
                     /locus_tag="VGR22_06295"
     CDS             741..1613
                     /locus_tag="VGR22_06295"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV2128209.1"
                     /translation="MALASRVPHVIPPGSQPVPIEQPRSHVAGTSRGAGRLRVAREVL
                     TIVLGALLYFFVRGLMETQVAVAFANAETLIALEQSLGLFHEPSMQGWITGDQWLVDL
                     ANRIYIFGHWPVIAGTMAWMVWKHRDRFAVYRSALLLSGAIGIVFFVLLPMAPPRFLV
                     DQGFVDTVTTHSEAYRVLQPPSFTNQYAAMPSLHVGWNLLMGIAIVRHASTLWFRAFG
                     YVMPLVMWLATIVTANHYILDGVVGSTVALTGLALAWRISRPRAAADEVTAIQPEPYQ
                     GIVIGQGWNRRPAA"
     gene            2110..2673
                     /locus_tag="VGR22_06300"
     CDS             2110..2673
                     /locus_tag="VGR22_06300"
                     /inference="COORDINATES: protein motif:HMM:NF018213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycolipid-binding domain-containing
                     protein"
                     /protein_id="HEV2128210.1"
                     /translation="MQREVIWQAEGGIGQEHLTLSDRQGVIVADSFAIVKPGPTPLRV
                     RYQVVLDEHWVVRGVAITTEIAHGSPTVIELRSDGKGNWTNSDGSPLPQLDGCIDIDI
                     SSTPFTNTLPIRRLDLREGQSEMVHAVYVNVETLRIEAWEQRYTRVDAGTVRYESATS
                     GFQRDLDVDEDGLVRNYPGLFTRVWSG"
BASE COUNT          487 a          982 c          995 g          541 t
ORIGIN      
        1 acgatgaagc gcaggcgatc ctgctcggcg aggccgtaga ggcggtaccc cgccggagtg
       61 cgcgcaggag caggcaccag accgatctgc tcatagtaac ggatggtctt ggggttgagg
      121 ttcaactgcg cggcaagttc ggcgatgcgc agcttcgtgg tgtgtgactg atccatgatg
      181 aacaccatag cacgcattac gagcatccgt gcaggagcgc tgcctgaccc tgaacaccgt
      241 gcttgtgctt tggggtagcg acgggtggat ctccgcggcc agcaccgtac atcgtcctcg
      301 acgaactggg ccaagcgcgt ggttgccgag ttgcactggt ggcttggacc gggcatcccg
      361 ttctggggga tagccctcca tcaggtgggc ggtggtcccg accccaaact ctcccgattc
      421 cctgctctcg aaaaccaatc tcgaggtgca ccatcgatgc tgtgatcgtc ggggtagatg
      481 taggcgttcc cagcggatat cacgttcagc gctcttcggc gttgtgcatg gcaagcgctg
      541 ccttcgatgc cgagatcgac ggttccggcg cgcgtctacg tcaggttacg taccaacccg
      601 ggaattcgtg cagcgcgagg aatacgcaat atcgccgatg tgcaggcgcc gccgcacagg
      661 catgatggac acatgacatc gccccggcga tgcacccgac ctcgccgagg cgcgtgaccg
      721 ttttcgagaa ggagaccacc gtggctcttg cctcccgcgt tccgcacgtc atccccccag
      781 ggtcccagcc tgttccgatc gagcagccac gatcgcacgt cgctggcacg agcagaggcg
      841 ccggtcgcct gcgggtcgcc cgcgaggtgc tgacgatcgt gctcggcgcg ctgctctact
      901 tcttcgtgcg cggcctgatg gagacgcagg tcgccgtcgc cttcgccaac gccgagacac
      961 tcatcgcgct ggagcagtcg ctcgggctct tccacgagcc gtcgatgcag ggctggatca
     1021 ccggtgacca gtggctggtc gatttggcga accggatcta catcttcggc cactggccgg
     1081 tgatcgcggg tacgatggcc tggatggtct ggaagcatcg cgaccggttc gcggtctacc
     1141 gctcggcact gctgctctcg ggcgcgatcg ggatcgtctt cttcgtgctg ctgccgatgg
     1201 ccccgccgcg cttcctcgtc gaccagggct tcgtcgacac cgttaccacc cacagcgagg
     1261 cctaccgggt gctgcagcca ccctccttca ccaaccagta cgcggcgatg ccgagcctgc
     1321 acgtcggctg gaacctgctg atgggcatcg cgatcgtccg gcacgcctcg acgctctggt
     1381 tccgcgcctt cgggtacgtg atgccgctgg tgatgtggct ggcgacgatc gtgacggcga
     1441 accactacat cctcgatggc gtcgtcggct cgacggtggc gctgaccggt ctggcgctgg
     1501 cgtggcggat cagccgcccc cgcgccgccg ctgacgaggt gacggcgatc cagccggagc
     1561 cgtaccaggg cattgtgatc ggccaaggct ggaatcgccg ccccgccgcc tgagtcagtg
     1621 attggcatgg agccccgctc ccttccccct ccgcggggct ccgtccggtc gatcccctcc
     1681 ctcctttctc cagccccgca ccttgggtgc ggggttttcc tgttggtacc gacgatcaat
     1741 aggggagacg agcgtaggcg atgtcatcct gagcgcaggc cgaaggccgg agtcgaaggg
     1801 tcccccgact ggcgctgctg cgccggtcgg tcccgttctc gtcgttcagt catgacaggt
     1861 gatcgttgat ttctgctgga gaacgagatc ggaccgccat gccaaacggc atggcgggag
     1921 attcttcgct gcgctcagga tgacacggat ttcgttgaac ctcacaagcg cgaccggttc
     1981 ccatcgtgga ggaaaccgga tctcagaatc cgggtctaca cgatggctgg tgcggctgat
     2041 cgggggctgg ggggcgcgtg tggtaccgtc gggcgcaggc atcgatacgg gtccatcagg
     2101 gaggcgggca tgcagcgcga ggtgatctgg caggcggagg gcggcatcgg gcaggagcac
     2161 ctgaccctca gcgaccggca gggtgtgatc gtggccgaca gcttcgcgat cgtgaagccg
     2221 ggtccgaccc cgctgcgcgt ccggtaccag gtcgtcctcg acgagcactg ggtggtgcgg
     2281 ggcgtggcga tcaccaccga gatcgcgcac ggctcgccga ccgtgatcga gctgcgcagc
     2341 gacggcaagg gcaattggac caacagcgat ggctccccgc taccccagct cgatggttgc
     2401 atcgacatcg acatctcctc gacacccttc accaacaccc tgccgatccg gcgactggac
     2461 ctacgggagg gccagagcga gatggtccac gccgtctacg tcaatgtcga gaccctgcgg
     2521 atcgaggcat gggagcagcg atacacgcgg gtcgacgccg gcaccgtgcg gtacgagtcg
     2581 gcgacgagtg gcttccagcg cgacctcgac gtcgacgagg acgggctcgt ccgcaactat
     2641 ccaggccttt tcacccgtgt ctggtcgggg tgatgcgttc agcccgtgcc gactcggctc
     2701 gaggtgatgg gctgttcgag attccgctga attgtcacag accgtagggg ccgacctccc
     2761 gcagcgagtc gtcggcccgg ccgtcgtcag gcggccaccg agagaacggc cggccaccgc
     2821 ttcgcaggtc gtcctctacc cggcggccgg cacgtgacag tttccacatg agtacacggc
     2881 ttcgctcaac cagcgcttgc ggccctgacc aggcgcacca tcaggtcgac ggcctcgtcg
     2941 ggccggaatc cctgctggcg aacgagcgag cgccaggttc cgaagtcgat cgcatgcccg
     3001 atcgc
//
LOCUS       DASYWL010000178         6597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_8645, whole genome shotgun sequence.
ACCESSION   DASYWL010000178 DASYWL010000000
VERSION     DASYWL010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6597
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8645"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VGR22_06305"
     CDS             <1..436
                     /locus_tag="VGR22_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128211.1"
                     /translation="VERDVPHTAIDMDWLRCSFPRPSGDPFNVELGCRNLADIARNIC
                     ALGVTRFVIADVVETRDQRERYCEAIPSAEVVIIRLTAGIERIRERIAQRQGAYPDPW
                     EPARAAELIRIMDESDVSDVAIELWCRSPREVAEEVVRWLGW"
     gene            complement(669..1634)
                     /locus_tag="VGR22_06310"
     CDS             complement(669..1634)
                     /locus_tag="VGR22_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128212.1"
                     /translation="MMEDQVRQDFERARHKAFLNDLKSTLIGRTNDLVPFKEIRSRLS
                     PESESYRGMQTVPVAQIVGSMDRFRDFDRTFLPRKKATQGRWTRIDRAYYEDIRLPPI
                     QLYKVGDIYFVKDGNHRVSVARERGVEFIDAEVIEGHVRVPLDSAMSPQELLLQTEYA
                     EFLRRTDLDLLEPEHDIRPTALGRYDEIWGHIQAHRAWLSSIWHREATVHEAVKDWYG
                     YIYMPIVRVARDRGVLDAFPRLTEADIYLWVMKHRGALGEEAGHDIGPVESAEDYATI
                     MTGDRGVGDRISAALGRTSRTPDAERERERRRRRLRRWPRPRGGR"
     gene            complement(1631..2356)
                     /locus_tag="VGR22_06315"
     CDS             complement(1631..2356)
                     /locus_tag="VGR22_06315"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV2128213.1"
                     /translation="MKLRILAVSDDVDQRIYNSSVRDRMGDVDMVIGCGDLPASYLEF
                     LSDALLRPVYYVLGNHAEELTRAGERGTPKHPEGCIDLGGKVVRDPGSGLILAGLPGS
                     VRYSQSDPVQYTEWEMRWMILKMAPLLFYNRMRYGRAVDILVTHSPPRHVGDREDPAH
                     QGFIAMRRFLRRFRPAYQLHGHVHLYDRSKPNVVRFMDTDVINVFPYQRLELVFEHLR
                     SSESTSTAASTSDRSAPEVEQSP"
     gene            complement(2440..3078)
                     /locus_tag="VGR22_06320"
     CDS             complement(2440..3078)
                     /locus_tag="VGR22_06320"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV2128214.1"
                     /translation="MRLLLIRHGQTHGNINRHLQGDDDPLTELGRTQALVTGRYLAAN
                     ESITHLYCSPLARAFETASIIGNAIRHEPMVEPGLAEINIGVAAGTPFDVWMADNPQH
                     IEQLRDPVTRMDFEWQGGENGHAFRDRVFAAWDHIVNEHQGLNHVVAVVTHGGALAWI
                     AARLQNLPDDTWPEDFFHNLSISEIDIARDGSTEVVAWNQTRHLEEAGVTVT"
     gene            complement(3497..4936)
                     /locus_tag="VGR22_06325"
     CDS             complement(3497..4936)
                     /locus_tag="VGR22_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128215.1"
                     /translation="MTTAGSLLESSQQSVSPPRLAAALVALVLAFGMTVAAITPAAAQ
                     DAATPEATPTQQQLEQILEQAIAEGVEQLTVPLQEFNDSGIDGTATFFDLGEETPIQI
                     DIEGGGDVVPAFILQGTCGETEPEPVERLTPVDETGESLSLIDRSLTELLEGEFTIEL
                     RLSPTQLGVLAACANIEGVPVPATPADATPEAETPTAVPTGEGGLVETPTPTPAVTPT
                     PAVTPTPAVTPAVTPTAAVTPTVTPVVGGKGTTIDGTGGADVAPETVASLPLDDYSGL
                     GVTGTVSLLALDDDTTKVKITLSGDAVTDGHIVHLHRGTCDNLQDEGTIYLESVGADG
                     ISETTVGMSLDALLTGGWSVNVHLSEEQWDTWLVCGYLGNATAGMTGVIDVTPVVGGK
                     GTAARTTTTDTGAAVGGTGDGTSGVSGKGESTRTSTLTQGVGVGSTLLWPDSPAKSVA
                     SALGVFALLLAGAGITLRHGQRTDRQPSR"
     gene            complement(5339..6160)
                     /locus_tag="VGR22_06330"
     CDS             complement(5339..6160)
                     /locus_tag="VGR22_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128216.1"
                     /translation="MTPPTHPLQEGGFDPLAMLRRAPNDGDDIPSIKLRMLWLRQRVP
                     EARVETSVVTLDDNVVVMAATIALPDGGEGSGHAAVRRIEGDDIAALIETTELRAIGR
                     ALDIIGYVVVDQQGGQIAPERAEREPVDREPTPITRERPDRAGQEEPSRPEPASEDQP
                     QGPATPPGHVQAIRSLRDRGTRDTAPQRPEPIPAPRQDRPTQRPEQQQSPSVSDAEPE
                     LEDVSWTAFWNWARGSYQLSSRVQLEELLGQSIGPKTPGQVRQLLLEHFSSEKTD"
     gene            complement(6195..>6597)
                     /locus_tag="VGR22_06335"
     CDS             complement(6195..>6597)
                     /locus_tag="VGR22_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128217.1"
                     /translation="VDHAQVLVIYGSGTSATIEGGSMMPESYPFTSRFEVLCDNGAVE
                     YHLRAGGRSFEVGEPTNELTIYRNEGEPEVVSVEQTDPYMNECAYFIACAREGRPPER
                     STPAAALDAMRVSLAAQASVDRGEPVAVRNA"
BASE COUNT         1088 a         2018 c         2303 g         1188 t
ORIGIN      
        1 ggtggagcgt gacgtgccgc acactgcgat cgacatggac tggctgcgat gctcctttcc
       61 gcgaccgtca ggagacccgt tcaacgtcga gctgggctgt cgcaacctgg ccgacatcgc
      121 gcgcaacatt tgcgcattgg gcgtcacgcg cttcgtgatc gccgatgtcg tcgagacgcg
      181 tgaccagcgc gagcgatatt gcgaggcgat ccccagtgcc gaagtggtga tcatccggct
      241 gacggccggc attgagcgta tccgggagcg catcgctcag cgccaaggcg cttaccccga
      301 tccatgggaa ccggcccggg ccgccgagct gatccgcatc atggatgaga gcgatgtcag
      361 cgacgtggcg atcgaactgt ggtgtcgatc gccacgcgag gtggccgagg aggtcgtgcg
      421 ctggctgggc tggtgatatc gccaaggatg tcatccgagc cggcgcggtg cgtgagcacc
      481 gatgccgtgt cgagggatcc cccggcgtgt agtgtcggtc ttttgctcat gtagtggaaa
      541 taggctctaa ggtgagaacg cggtgcccgc cctgccactt ggcagggcgg gcgatccttc
      601 gcttcgctca ggatgatagg atgatgggtc ggagggtgtg gaccttcagt atccggagat
      661 accgctgatt atcggccgcc gcgggggcgg ggccagcggc ggagacgacg acggcggcgt
      721 tcccgctcgc gctcggcgtc gggcgtgcgg ctggtgcgac cgagcgcagc gctgatgcga
      781 tcgccgactc cgcggtcgcc ggtcatgatc gtcgcgtagt cctccgctga ttcgaccggc
      841 ccgatgtcgt ggccggcctc ctcaccgagc gcgccgcggt gcttcatcac ccagaggtag
      901 atatccgcct cggtcagccg cgggaaggcg tcgagcacgc cgcggtcacg tgccacgcgc
      961 acgatcggca tgtagatgta cccgtaccag tccttgacgg cctcgtggac cgtagcctca
     1021 cgatgccaga tggacgagag ccaggcgcgg tgcgcttgga tgtgtcccca aatctcgtcg
     1081 taccggccaa gtgcggtggg acggatgtca tgctctggtt ccagcaggtc gagatcggtg
     1141 cgacgcagga attcggcgta ctccgtctgc aacagcagct cctgcgggga catagcggag
     1201 tccagcggca cacgaacatg cccctcgatc acctcggcgt cgatgaactc cacgccacgc
     1261 tcccgagcga ccgaaacccg atggttgcca tccttgacga agtagatgtc gcccaccttg
     1321 tagagctgga tcggcggcag gcggatgtcc tcgtagtagg cacggtcgat gcgcgtccac
     1381 cggccctgcg tggccttctt gcgtggcagg aacgttcggt cgaagtcgcg gaagcggtcc
     1441 atgctgccga cgatctgggc gaccgggact gtttgcatgc cacggtagct ctcggattcg
     1501 gggctgagcc ggctgcggat ctccttgaac ggcaccagat cattggtgcg cccgatgagt
     1561 gtcgacttga gatcgttgag aaaggccttg tggcgggccc gttcgaagtc ctgacggacc
     1621 tggtcctcca tcatggcgac tgttccacct ctggtgcaga ccgatcggac gtggatgctg
     1681 ctgtggaggt cgattccgat gatcgaagat gctcgaacac aagctcgagg cgctggtagg
     1741 gaaaaacgtt gatcacgtcg gtgtccatga agcgaacgac gttgggcttg gaacggtcat
     1801 agaggtgcac gtggccgtgc agttgatagg caggacgaaa ccggcgcagg aagcggcgca
     1861 tggcgatgaa cccttggtgg gcggggtcct cgcggtcgcc gacgtggcgt ggcggcgagt
     1921 gggtgacgag gatgtccacc gctcgcccgt accgcatccg attgtagaac aggagcgggg
     1981 ccatcttcag gatcatccag cgcatctccc actccgtgta ctggacggga tccgattggg
     2041 agtagcggac ggatccgggc agcccggcga gaatcagccc gctgccggga tcacgcacga
     2101 ccttgccacc gagatcgatg cacccctcag ggtgcttcgg cgtgccgcgc tcgccggcgc
     2161 gggtcagctc ctcggcgtgg ttgccaagca catagtagac aggtcgaagc agcgcatcgg
     2221 agagaaactc gaggtagctc gccgggaggt cgccgcagcc gatcaccatg tcgacatcgc
     2281 ccatgcgatc cctgaccgag gagttgtaga tgcgctggtc aacgtcatcg gaaacggcga
     2341 gaatccggag tttcatgcgc tggatgtccc gtcgaagacg tggtcgtgcg cgatacgggg
     2401 ctgacccggc ggctcaagca taccccacgc cctgccgaat caggtcacgg tcacgccggc
     2461 ctcttcgagg tgccgagtct ggttccaggc gaccacttcg gtgctgccat cgcgcgcgat
     2521 gtcgatctcg ctgatcgaga ggttgtggaa gaaatcctcc ggccaagtgt cgtccggcag
     2581 gttctggaga cgcgcggcga tccaggcgag tgcaccaccg tgggtcacca cggccacaac
     2641 gtggttgagt ccctgatgct cgttgacgat atggtcccac gccgcgaaga cacggtcgcg
     2701 aaaggcgtgg ccgttctcgc ctccctgcca ttcgaaatcc atacgggtga cggggtcgcg
     2761 cagctgctcg atgtgctggg ggttgtcggc catccagacg tcgaagggcg tgccggcggc
     2821 gacaccgata ttgatctcgg ccaggccggg ctcgaccatc ggctcgtggc ggatcgcgtt
     2881 gccgatgatc gaggcggtct cgaaggcccg ggcaagcggg ctgcagtaga ggtgggtgat
     2941 ggactcgttg gcggcgaggt agcgaccggt gacgagtgcc tgggtgcggc ccagttcggt
     3001 gagcgggtcg tcatcgcctt ggagatggcg gttgatgttg ccgtgagtct ggccgtggcg
     3061 gataagcagg agtcgcatag cgcaggatcc tctcggtcgg acgctgggat ggatgttcag
     3121 cgaaacggaa tcggcaagcg cggcctcggc cacgtcgcca accagtactg tacccgcatt
     3181 tcttgctgtt cggcctgttc tgctgtcgct tgtgtgttca tcaagggacc ttgcaaggcc
     3241 gggagtgggg agcgcctcgc cggctcggga cgacagcggt ggtcggcgtc agcaagggac
     3301 gctggtggct tgaggctctg gccgggtaga tgcgaaattc acccccacgg aatgcagcgt
     3361 tcgaaaggct gatccggcct gcagggaggg taccgatgac acaaaacggc gatggacccg
     3421 gcttctgccg ggcctatcgc gaaaagctgg aggagggagt ggatctgatc gggctggatc
     3481 agagtccgag tcgctgctag cgggaaggtt gtcgatcggt acgctgcccg tgccgaagcg
     3541 tgatgccggc accggcaagc agcagggcga agaccccgag tgctgaagcg acgctcttgg
     3601 ctggcgagtc cggccagagg agggtggagc caacgccaac accctgcgtc agcgtcgatg
     3661 tccgcgtgga ttctcccttg ccgctgacac cggaggtgcc atcgccggtt ccaccgacgg
     3721 cggcgccggt gtcggtcgtg gtggttcggg ctgcggtgcc cttgccgccg acaaccggag
     3781 tgacatcgat gacgccagtc atgcccgcgg ttgcattgcc gaggtacccg cagacgagcc
     3841 aggtatccca ctgctcctcg gacaggtgga cgttcacgct ccagccaccg gtcaacagcg
     3901 catcgagcga catgccgacc gtcgtctcgc tgatcccatc ggcgccgacg ctctcgagat
     3961 agatcgtgcc ctcatcctgg aggttgtcgc atgtcccgcg atgcaggtgg acaatatgcc
     4021 catcggtgac agcatcaccg gatagcgtga tcttgacctt ggtggtgtca tcgtcgagcg
     4081 ccagcagtga aacagtcccg gtcacgccca gcccgctgta gtcgtccagc gggagggacg
     4141 cgacagtctc cggcgcgacg tccgcgccac cggtgccgtc gatcgtcgtg cccttgccgc
     4201 ccacaactgg ggtgaccgtc ggtgtcacgg ctgccgttgg cgttacggca ggtgtcacgg
     4261 ctggggttgg cgtcacggcg ggtgtcggcg tgactgccgg tgtcggtgtt ggcgtctcga
     4321 cgagccctcc ctcaccggtc ggcactgccg tcggtgtctc cgcttccgga gtggcgtcgg
     4381 ccggcgtggc cggcaccggg acgccctcga tgttggcaca ggcagccagc acgccgagct
     4441 gggtggggga gagccgcagc tcaatggtga actcgccctc gagcagttcg gtgagggacc
     4501 ggtcgatcag tgacaggctc tcgcccgtct cgtcaaccgg cgtcaggcgt tccaccggct
     4561 ccggctcggt ctcgccgcag gtgccttgga ggataaaggc gggcaccacg tcgccgccgc
     4621 cttcgatatc gatctggatc ggcgtctcct cacccagatc gaagaacgtg gcggtgccat
     4681 cgatgccgga gtcgttgaac tcctggaggg gcaccgtaag ctgctcgact ccctcggcaa
     4741 tcgcctgctc gaggatctgc tcaagctgct gttgggttgg ggttgcctcg ggcgtcgccg
     4801 catcctgggc agcggctggc gtgatggcgg caacggtcat gccgaatgcc agcacgagcg
     4861 cgacaagcgc ggctgcgaga cgaggcggac tgacggattg ttgactggat tcaagcagtg
     4921 accctgcggt cgtcatgcag acaccctccc ccggcgcgtc caggtgacga ttggagcgcg
     4981 acggcccctc cggccgccat gcacatcccc agggtggtgc cgagcctgga tggaggccca
     5041 gaccagcagc ggagatgctg atcgatggca caccgggcgg gggccggtgt gcctgccggg
     5101 cgctgcagat gcggggctgg agcgcctgac atgaatcagg ctacacgtga ggatgggttg
     5161 tcacaagtgt acgtacgttg acgatttggc aagatcccga ggaggtgccg cggtgatgat
     5221 cgtgaaaaat aaggccacga ggtgtacgta tcgatccgaa actcaccgac gcaatgtacg
     5281 ttcatcactg acaactgggc acagcggcat cttgccgaat gttggcaatc agctggaatc
     5341 agtcggtttt ttcgctcgag aagtgctcga gcagtagctg gcggacctgg ccaggcgtct
     5401 tcgggccgat cgattggccc agcagttcct cgagctgcac acgggagctg agctgatagg
     5461 agccgcgcgc ccaattccag aacgccgtcc aggagacatc ctccagctcc ggctcggcgt
     5521 cgctcacaga aggagattgc tgctgctccg gacgctgcgt ggggcgatcc tgccggggag
     5581 cgggaatcgg ctcgggtcgc tgtggcgcgg tgtcccgcgt gccccggtcg cgcagggagc
     5641 ggatcgcctg cacgtgaccg ggcggggtgg ccgggccctg cggctggtcc tcactcgctg
     5701 gctccggccg cgagggttcc tcctgtccgg cgcgatccgg gcgctcgcgg gtgatcggcg
     5761 tcggctctcg gtcgacgggt tcccgctcgg cacgctcagg ggcgatctgg cctccctgct
     5821 ggtcgaccac cacatagccg atgatgtcca gcgcacggcc gatggcgcgc agctcggtcg
     5881 tctcgatcag tgcggcgatg tcatccccct cgattcgtcg gacggcggcg tgacccgagc
     5941 cctcgccgcc gtccggcagg gcgatcgtgg cggccatcac aacgacattg tcgtcgaggg
     6001 tcaccaccga ggtttccacc cgcgcttcgg gcacgcgctg gcgcagccaa agcatgcgca
     6061 gcttgatgct ggggatatca tcgccgtcat tcggggcacg acggagcatc gcgagggggt
     6121 cgaagccgcc ttcctgcagc gggtgggtgg gtggtgtcac gatgctcctc cgggccggta
     6181 cggggttgtc cgtttcaggc gttcctgacg gcgaccggtt cgccgcggtc gaccgacgcc
     6241 tgcgccgcga gggagacgcg catcgcatcc agcgccgcgg caggggtcga gcgttcgggc
     6301 ggcctgccct cgcgcgcaca ggcaatgaaa taggcgcact cgttcatata cggatcggtc
     6361 tgctcgacgg agacgacctc cggctcgccc tcgttgcggt agatggtcag ttcgttggtc
     6421 ggctcgccga cctcgaacga tcgtcccccg gcccgtaaat ggtactcgac cgcgccgttg
     6481 tcgcagagca cctcaaagcg ggaggtgaag gggtagctct cgggcatcat cgagccaccc
     6541 tcgatcgtcg ccgacgttcc cgagccgtag atcacgagta cctgggcatg atcgacg
//
LOCUS       DASYWL010000179        18936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_120939, whole genome shotgun sequence.
ACCESSION   DASYWL010000179 DASYWL010000000
VERSION     DASYWL010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 18936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18936
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120939"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /locus_tag="VGR22_06340"
     CDS             complement(<1..1124)
                     /locus_tag="VGR22_06340"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEV2128218.1"
                     /translation="MTSNGKIVGFDPRLSAALLIAKVTAAGIRLAGRGGGTSAPGLVA
                     DRIDSGMLGKLTRRLPGGVVMVAGTNGKTTTSRMIADLLAAAGMPVVHNRSGSNLVRG
                     VVSAFARQASVTGAPGGEVGVIESDEAALPQLLDRVTPRAVLLNNLFRDQLDRYGELD
                     AIARKWQPALKALDRETTVVVNADDPTLVALTTDLRAEVVRFGMDDEPHRLETLSHAA
                     DAVDCHVCGADLEYRALYVSHLGDWYCPNCGYHRPSLDVIGREVTLRGVTSLGMHVMV
                     DGEEVPIHVGVPGLYNAYNLVAAVAVGRTMAVEMPVITRAMDAFRSAFGRIERVVYRN
                     RTLTLALVKNPVGFNEVLRMLTAESGGIEVPTLIAINDLYA"
     gene            complement(1114..1929)
                     /gene="mreC"
                     /locus_tag="VGR22_06345"
     CDS             complement(1114..1929)
                     /gene="mreC"
                     /locus_tag="VGR22_06345"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEV2128219.1"
                     /translation="MTTISFRQTLSLVAIFVAVSATLIVMDRRDALDPLREGLISVVE
                     PVASGFASIARGPNFESDLERELERVEAERDAALAENANLKAMIAEYEVLDQERVVES
                     QRPELDFLAAKVIGGDPTGTQHFAWINKGSDDGVEVGMAVTDPDFYVGQVVEVTESTA
                     KVMFIVDTSASVGAQLLESGADGIVVGQWQLGGRLLLQNIDRSAEVEEGAVVVTSGAL
                     TTETRGVPPNIIIGTVFGEGEPTLRTNEVSYQVRPVVEFESLNTVWVVMPNDE"
     gene            complement(2007..3050)
                     /locus_tag="VGR22_06350"
     CDS             complement(2007..3050)
                     /locus_tag="VGR22_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEV2128220.1"
                     /translation="MSTLFGFFSRDLGIDLGTANTLVYVRGRGIVISEPSVVAIDTKT
                     KRALWVGMEAKAMMGKTPESIVAVRPLKDGVIADFDTVEMMLHYFIRKVHTQRLMAPH
                     PRVVIGIPSGVTEVEKRAAKDAALSAGAREAYTIEEPMAAAIGAGLPINEPIGSMIVD
                     IGGGTTEVAVISLGGIVVNHSIRVAGDEIDDALIQYARRDHNLVIGERTAENAKMAAG
                     SAYPLEEEVRVPLRGRDLLTGLPKSVEVSSVELRDAISQPVNTIIELVKNSIEETPPE
                     LVADIMEHGITLAGGGALLMGLDRRLQSELRMPVHRAEDPLTCVARGTGRVAEALHLY
                     QRALAGGQELRRG"
     gene            3329..4135
                     /locus_tag="VGR22_06355"
     CDS             3329..4135
                     /locus_tag="VGR22_06355"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEV2128221.1"
                     /translation="MAQQPTPLPEPDWSPLASCLAVIPSGMRVSLAARHLGTGSTLDY
                     DPDRPVQAASTIKTLILAALARAVDAGTLDLDMDVAVRADMRAGGSGVLNWLHEGISL
                     PLRDHAWLMIAISDNTASNAVIDTVGLSIVQQTTIELGVPSLRLGRRFYGYVPKDEIS
                     RNEATARGLADLLTSIWNDTAASPERCRWMRRLHGDQQHRDRLPRCLPDGISYAGKTG
                     TLDGISHDMGVIEGPSGAVAVAALIESSAGHYDDEVYLGRIGEAIGEIVS"
     gene            4253..5446
                     /locus_tag="VGR22_06360"
     CDS             4253..5446
                     /locus_tag="VGR22_06360"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEV2128222.1"
                     /translation="MRITGVQTFIVGNPWKNWLFARLETDQDGLYGIGEGTLNAFAKS
                     AEAVIHELTPRFVGTDPFQVETIIQRMTRDLYSEGGQLHMNAVAAIEVACWDIIGKVT
                     GRPIYDLIGGRYHESLPAYANGWYAGDRTPESFAERASEVVTRGYKALKFDPFGHNWR
                     MMTLAERHLSVDIVRAVREAVGPDVQLMIEVHSRFSVAEAIYVGQQIAEYEPTWFEEP
                     TGHQNIASTVEVARHLNIPVASGESLSSPQQFAELLSHDAVHILQPEPGAMGGIWRTR
                     QVAAMADAHYAVVAPHNAQGPISTATCIQISAACPNLLVQEIFDEYNVEWERDLVDRP
                     AEVIDGRIQIPNRPGLGVDLNWSELEKHPYEVTNFLPLFAPGWERREGERPPKEGPSV
                     RKTGA"
     gene            complement(5884..7095)
                     /locus_tag="VGR22_06365"
     CDS             complement(5884..7095)
                     /locus_tag="VGR22_06365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEV2128223.1"
                     /translation="MTSTKSQRQTVLEAIEAARQIILEAGDTIHANPEVGMEEHKAVE
                     LLTGIIASFGFEVERGVAEIETAFKARKRGNGEGPRIAYIAEYDALPGLGHGCGHNLL
                     ASSNLAAAIGLGAAVDELGGEVLFIGTPAEESHGAKAPMIEAGVFRDVDIAMSSHHGG
                     HVTFLPIRYPDGTCLAVSSRRFKFHGRTAHAAKDPETGINALNAVIQLFNGIDAMRQH
                     VTPDVRMHGIITDGGKAPNVVPDYAEAEFLFRAASRPAVDELVSRAEKVALGAGMMTG
                     AKLEIETPTPDYDDMLPNYTLSGLIRQQLDAIGLEETNRQPSSGPGAYSTDLGNVSRA
                     MPTTSLTFAISETPISGHTQDVVDASISELGRENALRTGKALALAGVELLANPELAKT
                     IRREFEEFRPA"
     gene            7223..7768
                     /locus_tag="VGR22_06370"
     CDS             7223..7768
                     /locus_tag="VGR22_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEV2128224.1"
                     /translation="MMLSTTTHDSDRATVPRIAFATLVERHGADLHRYAWSLTRNPED
                     ADDVYQETLLKAYKAWRRLPADANHRAWLFRIASNTWISDRRKNGRVHQMADDAPELP
                     DRGPDTVTQVAATDTLERVTHAIEALPPKQRAALVLRKYHDMSYDEIGEVLDSSGEAV
                     RANVYEALRKLRGQFAAALAD"
     gene            7784..8608
                     /locus_tag="VGR22_06375"
     CDS             7784..8608
                     /locus_tag="VGR22_06375"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEV2128225.1"
                     /translation="MTHEANPQHPTPSGSISTSLAVVDDPCEVVDEALPAMVIGDLTT
                     ADEHAIEEHCAVCRECAEIRGAWVMSLAEPDLPVDAAPPSPARALGLHEGHYGLMESP
                     LGDLLIAVTEDGIAEIGYLANHDREEELAELEARGILATERSATVEPVREQLREYFAH
                     RRNEFTLPVDLYGITPFTRQVLEATSRVPFGRVRTYQGIAQEIGKPKASRAVGNALGR
                     NPVPVIVPCHRVVRADGSMGWYTGGSHIKEKLLGIEGVAFPQKGRNQPVLPGLGEG"
     gene            complement(8935..9642)
                     /locus_tag="VGR22_06380"
     CDS             complement(8935..9642)
                     /locus_tag="VGR22_06380"
                     /inference="COORDINATES: protein motif:HMM:NF021365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2064 domain-containing protein"
                     /protein_id="HEV2128226.1"
                     /translation="MIVRRSDNLLMIAARAPHAGSTKTRLGRAIGMERAARLYVAFLA
                     DIAGCFTPPANPGGSWDLAWTHSPPDVDFRSELREAIGDAPAHVRYVPQIANQDWGRR
                     QSALLAWGDAQGYRRTVLIASDSPQIEVAVIEDAFAALASHDVVLGRVHDGGYYLIGQ
                     RGFREILDGVQMSTASAADGVLQHARNQGLATAEVDPTFDVDVEDDLTLLIEHLAASD
                     GTAAPATWRAMKDLGLR"
     gene            complement(9639..10430)
                     /locus_tag="VGR22_06385"
     CDS             complement(9639..10430)
                     /locus_tag="VGR22_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV2128227.1"
                     /translation="MSVQARLSATVVIPCLNEEESIGGLVEDIAAVRDDPATPIEITM
                     ILVVDNGSTDDTALVARQAGACIITEPRRGYGRACLSGVLASGPVDYIVLMDGDRSDV
                     PDDLPKLLEPLLSGDADLVVGSRTLGSYEKGSLHPQQIAGNKVAALLARWFYGVRLTD
                     IGPFRVIRKRNLLELGMREMTYGWSIEMIIRAKRKGLTVTEVPVRYRRRAGGSSKVSG
                     NLKGTVKAGYRIIAAIFRARKDDRPLPDGLAAWQERHAASDEAAS"
     gene            complement(10427..11566)
                     /locus_tag="VGR22_06390"
     CDS             complement(10427..11566)
                     /locus_tag="VGR22_06390"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEV2128228.1"
                     /translation="MAETTTALITGGAGLIGSHIVDAALAEGWKVRVLDSLEPQTHAY
                     GRPGWLPEEAEFIQGDVRNPDDWQRALRGVDIVFHEAAYGGYMPEIAKFIHVNSYGTA
                     LMLETIRNHDLPVRKLVLASSQAVYDEGAYACPTHGHFYGETRPIEQLESGDYAMHCP
                     ICGAESTPVPTDETAPMGGENVYAISKADQERLVISFGRATGLPVTALRYSCTYGPRQ
                     SLFNPYTGVISIFCTRLMNDLPPVVYEDGHQSRDLVFVGDVARANLLVATDSRADGQV
                     FNVGTGNAAEIGDLARLLARKLGKDIEPDLPGEYRPGEMRALISDTRRIQELGFAPQI
                     SLEGGIDRYLAWIETQGSVREYFSEAERLLRERRVVKRAKTGRAS"
     gene            complement(11644..12825)
                     /locus_tag="VGR22_06395"
     CDS             complement(11644..12825)
                     /locus_tag="VGR22_06395"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEV2128229.1"
                     /translation="MTPRDPGPSLRTRAARTGHHNGDGDTWPVVHPIVTSTALAGRDP
                     DTLVNRIGRNEPTYHRAHFPNAARLEEAVAELEGAEAGYATSSGMAAVTTVVLALLGQ
                     GDHLIVGAGGYSDSEELLTRELARFGIGGSVVPLDDLGAVRNAIRPQTTLIFAETITN
                     PGITVPDLGALAGITRERGVTLVVDSTVATPMLCRPLEHGADLVIHSVTKFLGGHHDL
                     SAGVIVGSRFLIDRIARVGYLLGAVPGAHDSALALRGIHTLAPRMAWISESADRIARY
                     LAEQPEVACVRYPGLATGAEGERVRRMLPDGRSGLMVVEFNGTATQAPAAAFVRSLRM
                     IPYVTSLGGEITTVCYPPRILADGDPETAGVGTRLRLSVGLEDPEDLIADLNGAFEAM
                     R"
     gene            complement(12837..14999)
                     /locus_tag="VGR22_06400"
     CDS             complement(12837..14999)
                     /locus_tag="VGR22_06400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEV2128230.1"
                     /translation="MVLYPSVVALRACAGPVLVAEVTLGSCATRIESEGAVTERRPDA
                     EPGIQRPSSENGEGLFRPTPPIAREPEAPSPDETEVTAPIVPQPSPPPPSRARQARDA
                     VQTVLHNWPFAVVLTLFAAGAMVVPTMTQIATTDDWAYTRSVEILLDEGRLTVFPVVA
                     ATAVGQVLWGALFGIIFGMSLGMMRVSTVVMVALGGIALYAILRQLGVTRSRSALGMA
                     VWLFNPLTYALAFSFMTDAHLASVMLISLAFYLRGLQPEREHLGFVLAGAFFAGFAFL
                     IRQQGALIPLAVAMWLFLSGRLWFRRRDIRRLLAVALLPLLMALAYYAWLRWFNDVPD
                     VQEDFLQDIFDKGWEATWLLVRRIPLYAIFYGALLLVPVLLAIRPWRLHRYSTSLFPT
                     PGGFYAFAGWSALILAGLYLAGTRGQAMPFAPQFVGPGGLGPPDVLGSRERLWERGSD
                     FAVALTIVSAIGALLLGMVLLRKLFAGRLPERWGFWLVASVGLWQLIGVFPPSYHYTN
                     RGVTLDRYLLPVIAVLIPLVFYALRDVDLFQPIAWLALAAVAVVSVAGVRDYLVFMDA
                     IWDMAETANENGVPIDRLDAGSGWDGYHLYTVMLDENITEARSPAGSPWWIYFYAKPT
                     DSSYIVATDPAVRRGYVVAEEREYGQWLDDDPVHVYLLRRWFLPYPIRWAGEEQSGGR
                     LLPGPGSQTPPDSTVVVATPRATTAATPAGSPMSATPE"
     gene            15156..15995
                     /locus_tag="VGR22_06405"
     CDS             15156..15995
                     /locus_tag="VGR22_06405"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HEV2128231.1"
                     /translation="MTRIHGRTFYFACQLLPLTERRCIHAIYAWCRHADDIVDTAAPG
                     GIAAAHEQLDRWLATIDRPVDPISHAFAWVIREHGVPTEPAQDLIAGVRMDLERASFE
                     TWDDLRWYSYCVAGTVGLMTVPILGCIDRSALPHAVNLGIAMQLTNILRDVAEDAEMG
                     RVYLPDEDLARFGVSRESLLAMNPDGDFRGLMRFEIERARGLYRSAHTGIPALSRAGQ
                     ITTIAGSHLYREILHRIEERGYDVFDGRATVSTRRKMRKMPLVVAAFLRMQLSLVGTR
                     PRA"
     gene            complement(16218..16973)
                     /locus_tag="VGR22_06410"
     CDS             complement(16218..16973)
                     /locus_tag="VGR22_06410"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEV2128232.1"
                     /translation="MTASGPAYEIGQQVLRRGGRVVLDRQATLDIAGAEHIPASGPCV
                     IVARHYHNLLDGCALYAATGRSLHILVGLDWAGTGLLRRVMEKLSRMVAWPIILRPDA
                     LDREAAAPKRKSEARRLLRAATRDGVDLLAAGKVVVMFPEGYPVVDPHAKTQRASPEG
                     VVPFQPGVVRLVRIAERRLGREIPLVPVGFAYEQRDDGRWEIAMRIGPPLSREGLESD
                     TELLRVLESQVAALSGIPVPARTRVASEQNVSR"
     gene            complement(16970..17878)
                     /locus_tag="VGR22_06415"
     CDS             complement(16970..17878)
                     /locus_tag="VGR22_06415"
                     /inference="COORDINATES: protein motif:HMM:NF016155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HEV2128233.1"
                     /translation="MQQATVDTVTRHAAHKSVSWLGDRRPLQIAKALLIGHLAALVFG
                     LAGMLIAIPNPDLWADSEVGLRVYDFGMRYAGSLHIIFGATAMFFWGIAAIGIRRTAI
                     FFAAAVPISLTSELLGTSTGEPFGNYAYTNFLGYKVLDHVPFSIPLSWFYVGFATYMI
                     GHVLAAQWNLRPRAIYAVIFGAWLLTVWDLVLDPAMAHESLSVKFWAWDETGPYFGMP
                     TQNFIGWTLTAVVFMTVARLLWRADPSGGGHRLAIWFPVAVFAANIVFASVLSASVDL
                     WVPILLAVMLGLLPSLFALRGSRGAA"
     gene            complement(17902..>18936)
                     /locus_tag="VGR22_06420"
     CDS             complement(17902..>18936)
                     /locus_tag="VGR22_06420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV2128234.1"
                     /translation="PDDGSVSVIVPVLNEEHRLEPCLAGLSRQGEAVREILVVDGGST
                     DRTRALAEAWTVRDPRIRFINASPVPDGWNGKPWGLHTGAISADLGSRWLLTIDADVR
                     PEPMLATSLLAHAERRALGALSVATPQRVSGQIEAPVHTSLLATLVYRYGIPGQVFED
                     PDAVQANGQCLLIERSALDTAGGFAAVAQSLVEDVTLARRCVRVGIRYGFFEPACDAS
                     LVTVEMYADWYDALSSWSRSLPMRDRDSGMPWASRMADLTFAMGVPVPMLILAAIWRR
                     MPLRSLAIRLNAGLLATRIGTQAGMARAYVALPATHWLAVLLDPVAVTILVGQARRRE
                     HTWRGRVVRW"
BASE COUNT         3335 a         6313 c         6272 g         3016 t
ORIGIN      
        1 gcgtagagat cgttgatcgc gatcagcgtc ggcacctcga tcccgccgct ctcggcggtg
       61 agcatgcgca acacctcgtt gaacccgacc gggttcttga cgagcgcgag ggtgagtgtg
      121 cggttgcggt agacgacgcg ctcgatgcgg ccgaaggccg agcggaaggc gtccatcgct
      181 cgcgtgatga ccggcatctc gacagccatc gtgcgaccga cggcgaccgc cgcaacgagg
      241 ttgtaggcgt tgtagagccc tggcaccccg acatggattg ggacctcctc gccatcgacc
      301 atgacgtgca taccaagcga ggtgacgccg cgcagcgtca cctcgcggcc aatcacatca
      361 aggctcggac ggtggtagcc gcagttcggg cagtaccagt cgccgaggtg ggagacgtag
      421 agcgcgcggt actccagatc agcgccacag acgtgacaat cgaccgcgtc cgccgcatgg
      481 gagagcgttt ccagccggtg gggctcgtcg tccatgccga agcggacgac ctcggcgcga
      541 aggtcggtcg tgagcgccac cagcgtggga tcatcggcgt tgacgacgac cgtcgtctcc
      601 cgatcaagcg ccttgagggc cggttgccac ttgcgggcga tcgcgtccag ctcgccgtag
      661 cgatcgagct ggtcacggaa gaggttgttg agcagcaccg cgcgcggcgt gacgcgatcc
      721 agcaactgcg gcagcgcggc ctcgtctgac tcgatcaccc cgacctcgcc gccgggcgcg
      781 ccggtgacgc tcgcctgcct ggcaaacgcg gagacgacgc cccgcacgag gttggaaccg
      841 gagcgattgt gcaccaccgg catcccggct gccgccagca gatcggcgat catgcgggag
      901 gtggttgtct tgccgttggt gccggcaacc atcacgacgc cgccgggcag ccgacgcgtg
      961 agtttgccga gcatgccgga gtcgatgcgg tctgcgacga gtcccggcgc ggaggtgccg
     1021 ccgccgcgac cggccagccg gatcccggcc gccgtcacct tggcgatcag cagcgccgcc
     1081 gagaggcgcg ggtcgaatcc aactatcttg ccgttactcg tcatttggca ttaccaccca
     1141 caccgtattg aggctctcga attccacgac cggccgcacc tggtaggaga cctcgttggt
     1201 gcgcagcgtc ggctccccct ccccgaagac cgtgccgatg atgatgttgg gcggcacccc
     1261 gcgtgtctcg gtggtgagcg cgccggaggt caccactacg gcgccttcct cgacctcggc
     1321 ggagcggtcg atgttctgta gcagcaaccg gccgccgagc tgccactgcc cgacaacgat
     1381 accgtcggcg ccgctctcga gcagctgcgc gccgacgctg gcgctcgtgt cgacaatgaa
     1441 catgactttg gcagtggatt ccgtcacctc aaccacttgg ccgacataga aatccgggtc
     1501 ggtgacggcc atgccgacct cgacgccgtc gtcactgccc ttgttgatcc acgcgaagtg
     1561 ctgcgtgccg gtcgggtcgc caccgatgac cttggcggcg agaaaatcca gctccggtcg
     1621 ctgcgactcg acaacccgct cctgatcgag cacctcgtac tcggcgatca tcgccttgag
     1681 gttcgcgttc tcggccagcg cggcgtcgcg ctcggcctcg acccgctcca gctctcgctc
     1741 cagatcggat tcgaagttcg gaccgcgtgc gatggatgca aagcccgatg cgaccggctc
     1801 cacgacactg atgagcccct cgcgcagggg gtcgagtgcg tcgcgccggt ccatcacgat
     1861 cagcgtggcg ctgaccgcga cgaagatcgc cacaagcgag agggtttgtc gaaacgagat
     1921 cgtggtcatg cgtcgccctt cgtcccgcaa ggcagccacg cgtggccaca ctgacggatc
     1981 gggtagatac cgtgtgagct gggaggctag ccgcggcgga gctcctgccc gccggccagc
     2041 gcacgctggt agaggtgcag cgcctcggca acgcgtccgg tcccgcgcgc cacacaggtg
     2101 agcgggtcct cggcgcggtg caccggcatg cgcaactcgc tctgcagtcg gcggtccagc
     2161 cccatcagca gcgcaccacc gccggcaagg gtgatgccgt gctccatgat gtcggcgaca
     2221 agctcgggcg gggtctcctc gatcgagttc ttgaccaact cgatgatggt gttgaccggc
     2281 tgcgagatag cgtcgcgcag ctccacggag ctgacctcga ccgacttcgg caggccggtc
     2341 agcaggtcac gcccacgcag tggcacgcgg acctcctcct cgagcgggta ggccgacccg
     2401 gcggccatct tggcattctc ggcggtgcgc tcgccgatca cgagattgtg gtcacggcgc
     2461 gcgtactgga tgagcgcatc atcgatctcg tcaccggcga cacggatcga gtggttgacc
     2521 acgataccgc ccagcgagat gacggccacc tcggtggtgc caccaccgat gtcgacgatc
     2581 atgctgccga tcggctcgtt gatgggaagg ccggcgccga tcgcggcggc catcggctcc
     2641 tcaatggtgt aggcctcgcg ggcccccgca gagagggcgg cgtccttggc ggcacgcttc
     2701 tcgacctcgg tcacgccgct ggggatgccg atcaccacac gcgggtgagg cgccatgagg
     2761 cgctgcgtgt gtaccttgcg gatgaagtaa tgcagcatca tctcgacggt gtcaaaatcg
     2821 gcgattacac catccttgag gggtcgaacg gcgacgatcg actcgggcgt cttgcccatc
     2881 atcgccttcg cctccatgcc gacccagagg gcgcgcttgg ttttcgtgtc gatggctacc
     2941 acagagggct cggagatcac aataccccgg ccgcggacgt aaaccagcgt gttcgctgtc
     3001 ccaaggtcga taccaagatc ccgactgaag aagccgaaca gcgttgacat ggggcggaac
     3061 aagacccgag acctccggga gcaccgtgcg atgggccgtc catgacgcac aggcggcaac
     3121 atacgtgtcg tgggcaacga tgggaccact atgcgggtcg atggagaaga gcgaaccgca
     3181 gaagactgca acagacccgg cgtctctcca taattcacgt ggcagtatat catgcagtct
     3241 gaacggccct tgcttccaga tgccatcccc gccaccatac cagaaagtgc gctggacaca
     3301 tcgactcgtg gaaaggactg ccgcccggat ggcgcaacaa ccgaccccat tgccggaacc
     3361 tgactggagt cccctcgcat cctgtctggc ggtgatccca tcggggatgc gtgtctcgct
     3421 cgcggcgcga caccttggca caggcagcac actcgactac gatccggacc ggccggttca
     3481 ggcggcaagc acgatcaaga ccctgatcct cgccgccctt gcccgcgcgg ttgatgccgg
     3541 tacgcttgat ctcgacatgg acgttgccgt tcgtgccgac atgcgcgccg gtggcagcgg
     3601 tgtcctcaac tggctgcatg aggggatctc gctgccgttg cgcgaccatg cgtggctgat
     3661 gatcgccatc tccgacaata cggcctccaa tgccgtgatc gacaccgtcg ggctcagcat
     3721 tgtgcagcag accaccatcg agctcggggt cccctcgctg cgcctcgggc gccgctttta
     3781 cggctacgtg ccgaaagacg agatcagccg gaacgaggcc actgcgcgtg gcctggccga
     3841 tctgctcacg tcgatctgga acgacaccgc cgcgtcgccc gagcgatgca ggtggatgcg
     3901 gcgactccat ggcgaccagc agcaccgcga ccgactccct cggtgtcttc cggacggtat
     3961 cagctacgcc ggcaagaccg gcacgctgga cggcatctcg cacgacatgg gcgttattga
     4021 ggggccatcg ggggcagtcg cggtcgccgc gctgatagag agctccgccg gccactatga
     4081 cgacgaggtc tacctcggcc ggatcggcga ggctatcggg gagatcgtgt cgtgagacac
     4141 cacatgttgt aatggagcac caagggaatg tcatcctgag cggagcgaag gatgacaggt
     4201 tggttgacgc ccaccgtctg ttcgcacggc aacacgcgaa aggactcgtc acatgcgcat
     4261 caccggcgtt cagaccttca tcgtcggcaa cccgtggaag aactggctct tcgcacggct
     4321 ggagaccgac caggacggcc tctacggcat cggcgagggc acgctcaacg ccttcgccaa
     4381 atccgccgaa gccgtcatcc atgagctcac gccacgcttt gtcggcaccg acccgttcca
     4441 ggttgagacc attatccagc gcatgacccg cgacctctac tctgagggtg ggcagctgca
     4501 catgaacgct gtcgccgcga ttgaggtcgc ttgctgggac atcatcggca aggtcaccgg
     4561 acggccgatc tatgatctca tcggcgggcg ctaccacgag tcgctcccag cctacgccaa
     4621 cggttggtat gcgggcgacc gcacaccgga gtcctttgcc gagcgtgcct cggaggtggt
     4681 cacccgcggc tacaaggcgc tcaagttcga tccattcggc cacaactggc ggatgatgac
     4741 gctggccgag cggcacctct cggtcgacat cgtccgcgcc gtgcgcgagg ccgtcgggcc
     4801 ggacgttcaa ctgatgattg aggtccacag ccgcttcagc gtagccgagg cgatctacgt
     4861 cggccagcag atcgccgaat acgagccgac gtggttcgag gagccgacgg gccaccagaa
     4921 catcgccagc acggtcgagg ttgcccgaca cctgaacatc ccggtggcat ccggcgagag
     4981 cctctccagc ccgcagcagt tcgccgagct gctctcgcac gatgcggtgc acatcctgca
     5041 gccggagccg ggcgccatgg gcggcatctg gcgcacgcga caggtcgcgg cgatggccga
     5101 cgcccactac gcagtcgtcg caccgcacaa cgcgcagggg ccgatctcga cggcaacgtg
     5161 catccagata agcgccgcct gcccgaacct tctggtgcag gagatcttcg acgagtacaa
     5221 cgtcgagtgg gagcgcgacc tggtcgaccg cccggcggag gtgatcgatg gtcggatcca
     5281 gatcccgaac cggccggggc tgggcgtcga tctcaactgg tcggagctgg agaagcatcc
     5341 gtacgaggtg acgaacttcc tgccgctctt cgcaccggga tgggagcgcc gcgagggcga
     5401 gcgcccgccg aaggaggggc catcggtcag gaagaccggc gcgtagcgat cagatcagga
     5461 aacatgtcat cctgagccga aggcagagcc gagtattccc actccatggc caattcagag
     5521 tgatatggaa tccggctgat cggttggggc aagcgactcg taggggcgga tcttgtccgc
     5581 ccgtccggct tgctccgttc acctacggat tacccggatt ccatatgaga gtgccagcaa
     5641 aggcggtgtc attctgggcg cggcgaagaa tcccccgccc gaagggtggt ctgatctcca
     5701 acaggccaca cggaggtgac cctcgatcac agccaacggc gagagaggac cggcgctccg
     5761 cgccgggaga tccttcgaca cggcttcggc gccacggcgt cttgccggct caggatgaca
     5821 tcgcttcgtt ggtctatagt atcggctgcg ggaatccatg tcgcttctcg gacagggctg
     5881 cggctacgcc ggccggaact cctcgaactc acggcgaatc gtctttgcca gctcgggatt
     5941 ggcgagcagt tccacccccg ccagcgcgag cgccttgccc gttcgcagcg cgttctcccg
     6001 gcccagctcc gagatcgagg catcgacgac atcctgtgtg tgcccgctga tcggcgtctc
     6061 gctgatcgcg aaggtgagcg acgtcgtcgg catcgcccgg ctgacgttgc cgagatcggt
     6121 cgagtacgcc cccggaccgc tggacggttg ccggttcgtc tcctccaacc cgatcgcgtc
     6181 gagctgctgg cggatcaatc cgctgagcgt gtagttcggc agcatgtcgt cgtagtccgg
     6241 tgtcggggtc tcgatctcga gcttcgcgcc ggtcatcatc cccgctccga gcgcgacctt
     6301 ctctgcccgg ctgaccaact cgtcgaccgc tgggcgcgac gccgcccgga agaggaactc
     6361 cgcctcggcg tagtcgggca cgacgttggg tgccttccca ccgtcggtga tgatgccgtg
     6421 catgcgcacg tccggcgtga cgtgctggcg catggcgtcg ataccgttga agagctggat
     6481 cacggcgttg agcgcattga tgcctgtttc cgggtccttc gcggcgtgtg cggtgcggcc
     6541 gtggaacttg aaccggcggc tggagacggc gaggcaggtg ccgtcggggt atcgaatcgg
     6601 caggaacgtg acgtgcccgc cgtggtggct cgacatcgcg atgtcgacat cgcggaacac
     6661 cccggcctcg atcatcggcg ccttggcgcc gtggctctcc tcggccggcg tgccgatgaa
     6721 gagcacctcc ccgcccagct cgtccacggc cgcaccaaga ccgattgctg cggcgaggtt
     6781 gctgctggcc agcaggttgt gcccgcagcc atgcccgagg ccgggcagcg cgtcgtactc
     6841 ggcgatgtac gcgatgcgcg gcccctcgcc attcccccgc ttgcgcgcct tgaaggcagt
     6901 ttcgatctcg gccacaccgc gctcgacctc gaacccgaac gacgcgatga tgccggtcaa
     6961 cagctcgacg gccttgtgct cctccatgcc gacctcgggg ttggcatgga tggtgtcacc
     7021 ggcctcgaga atgatctgcc gggccgcctc gatcgcctcc agaacggtct gtcgctggct
     7081 cttggtggat gtcatgcgtg gtcatgctcc tgtgctatgg acgacgtctt cgcatgatct
     7141 cacacatgcc gcccgtctgc gtcaatgggg cagaatggga ctgaacctct cccgacagcg
     7201 cgcgtttcag ggagctcgcc tcatgatgtt gtcgacgacc acccacgact cagaccgggc
     7261 aaccgtgcct cgcatcgcct tcgccaccct cgtcgagcgc cacggcgcag acctgcaccg
     7321 gtacgcctgg tcactgaccc gtaatccgga ggatgccgat gatgtctacc aggagacgct
     7381 gctgaaggcc tacaaggcat ggcggaggct cccggcggat gccaaccacc gcgcctggct
     7441 gttccgcatc gcctccaaca cgtggatcag cgaccgccgg aagaacggcc gggtgcatca
     7501 gatggcggac gatgcgccgg agctgcccga tcgcgggccg gacaccgtca cgcaggtggc
     7561 cgccacggac acgctggaac gagtgacgca cgcgatcgag gcgctcccgc cgaagcaacg
     7621 ggcggcactc gtcctgcgga agtaccatga catgtcgtac gacgagatcg gcgaggtgct
     7681 cgatagcagt ggtgaggcgg tgcgggccaa tgtctatgaa gcactccgca agctccgcgg
     7741 ccaatttgcc gcagcgctgg ccgactagaa ggacctgacg accatgacgc atgaagccaa
     7801 cccgcaacac cccaccccgt ccggctcgat cagcacctcg cttgccgtcg tcgatgatcc
     7861 ctgcgaggtg gtggacgaag cactcccggc catggtgatc ggcgatctga ccaccgccga
     7921 cgagcatgcg atcgaggagc actgcgcggt ttgccgggag tgcgccgaga tccgcggcgc
     7981 gtgggtgatg agcctggcag agccggatct gccagtcgac gcagccccac cctcgcccgc
     8041 gcgagcgctc ggactccacg agggccacta cgggctcatg gagagtccac tgggtgacct
     8101 gttgatcgcc gtgacggagg atggcatcgc agagatcggc tacctagcca accatgaccg
     8161 cgaagaggag ttggccgagc tcgaagcacg cggcatcctc gccacggagc gctcggcgac
     8221 ggtggaaccg gttcgggagc agctgcggga atactttgcc caccgccgga acgagttcac
     8281 gctgccggtc gacctctacg gcatcacgcc gttcactagg caggtgctgg aggcaacgag
     8341 ccgtgtgccc ttcggccgtg ttcgcacgta tcagggaatc gcgcaggaga tcgggaagcc
     8401 gaaggcatcg cgggcggtcg gcaacgcgct cgggcgcaac ccggtgccgg tgatcgttcc
     8461 ctgccaccgt gtggtgcggg cagatgggtc gatgggctgg tacaccggcg ggtcgcacat
     8521 caaggagaag ctgctcggca ttgagggagt agccttcccg cagaagggcc gaaaccagcc
     8581 cgtgctcccc ggcctcggcg agggctgaat cacaccagca tgtcgcagga gtgggcccca
     8641 tccagatctc cgtcgttact atctctggca taccgagtga tctggagatg aatcgctgaa
     8701 tttgcgtgcc tgtaatggca ggccgggtcc gagcctggca gccaacagcg agtgtcattt
     8761 cgatcggagc gcccgaaggg tgcggagcgg agaaatctct tccgacgtgg atggctgata
     8821 tccgtcaggc aacagatttc tcggctgcag gctcgctgcg ctcgccttcc gctcgaaatg
     8881 acaggcaggg gaaggcagca tcttcatgta tcttcaccgg atgactgtcg tcacctacct
     8941 cagtccaaga tccttcatgg cgcgccaggt ggcgggggcg gcggtgccat cggaggcagc
     9001 gaggtgctcg atcaggagcg tcaggtcatc ttcgacgtcg acgtcgaacg tggggtcgac
     9061 ctctgccgtg gcgagcccct gattccgcgc gtgctgcagc acgccgtcgg ccgccgaggc
     9121 ggtactcatc tgcacgccat cgagaatctc ccgaaacccg cgctgcccaa taaggtagta
     9181 cccgccatcg tggacgcgcc caagcacgac gtcatgcgat gccagcgccg cgaacgcgtc
     9241 ctcgatcacc gcgacctcaa tctgtggcga atcggaggcg atcagcaccg tccttcggta
     9301 cccctgcgcg tcaccccacg ccagcagtgc gctctggcgg cgcccccagt cctgattggc
     9361 gatttgcggc acgtaacgca catgcgctgg cgcgtccccg atcgcctccc gcaactcact
     9421 tcggaagtcg acatcgggtg gcgagtgagt ccacgcgagg tcccatgagc cgccggggtt
     9481 cgcaggaggc gtgaagcatc cggcgatatc ggcgaggaaa gccacgtaga gccgtgcggc
     9541 acgctccatg ccgatggcgc ggccgaggcg cgtcttggtg gagccggcat gcggcgcccg
     9601 ggccgcgatc atcagcaggt tgtcgctgcg ccggacgatc acgaggcagc ctcatcgcta
     9661 gcagcgtgcc gctcctgcca agcagcgaga ccgtccggca gcggacggtc gtccttccgc
     9721 gcccgaaaaa tggctgcgat gatgcgatag ccagccttga cggtgccctt gaggttgccg
     9781 ctcaccttgg agctgccacc ggcacgcctg cgataccgga ccggcacctc cgtcacggtc
     9841 aagcctttgc gcttggcccg gataatcatc tcgatcgacc agccgtaggt catctcccgc
     9901 atgccgagct cgagcagatt ccgcttgcgg atcacgcgga acgggccgat gtcggtaagg
     9961 cgcacgccat agaaccaccg ggcaagcaac gccgcgacct tgttgccggc aatctgctgg
    10021 ggatgcaggg atcccttctc gtaggagcca agcgtccgcg agccgaccac gagatcggca
    10081 tcgcccgaca ggagcggctc cagcaacttc ggcaggtcgt cgggcacgtc ggagcgatcg
    10141 ccgtccatca ggacgatgta gtcgaccggg ccactcgcca gcacgccgct caggcaggcg
    10201 cgtccgtagc cacggcgcgg ctcggtgatg atgcacgccc ccgcctgccg cgcgaccaat
    10261 gcggtgtcgt cggtgcttcc gttgtcgacg acgaggatca tcgtgatctc aatcggcgtg
    10321 gccgggtcgt cgcggacggc ggcaatgtcc tcgaccagtc ctccgatgct ttcctcctcg
    10381 ttgaggcaag ggatcacgac ggttgcggag aggcgtgcct ggacgctcac gaagccctcc
    10441 cggtcttcgc acgcttgacg acgcgccgct cccgcagcag gcgctctgcc tccgagaagt
    10501 actcgcggac gctgccttgg gtctcgatcc acgccaggta ccggtcgatc cccccctcca
    10561 aggagatctg cggcgcgaag ccgagctcct gaatgcgacg ggtatcgctg atcagcgccc
    10621 gcatctctcc cggacgatac tcgccgggca ggtcgggctc aatgtccttg ccgagcttgc
    10681 gcgccagcag ccgggccaga tcgccgatct cggcggcgtt cccggtcccg acattgaata
    10741 cctgcccgtc ggcgcgcgaa tcggtcgcaa cgagcaggtt cgcgcgagcg acatccccga
    10801 cgaagacgag gtcgcgcgat tggtggccgt cctcgtagac gaccggcggc aggtcgttca
    10861 tcagccgggt gcagaagatc gagatcacgc cggtgtaggg gttgaagagc gactggcgcg
    10921 gcccgtaggt acaggagtag cgcagcgcgg tcactggcag gccggtcgcg cggccgaacg
    10981 agatgacgag ccgttcctga tcggccttgc tgatcgcgta gacgttctcg ccgcccatgg
    11041 gagcggtctc atcggtggga accggtgtcg attccgcccc acagatcggg cagtgcatgg
    11101 cgtagtcacc ggactccaac tgctcgatcg ggcgcgtctc accgtagaag tgaccatgtg
    11161 tcgggcacgc gtaggcgccc tcgtcgtaga ccgcttgcga cgaggccagc acgagcttgc
    11221 gcacgggcag gtcgtggttg cggatcgtct ccagcatcag ggccgtgccg taggagttga
    11281 cgtggatgaa cttggcgatc tccggcatgt acccgccgta ggctgcctcg tggaagacga
    11341 tgtcgacgcc gcgaagcgcc cgctgccagt cgtcggggtt gcggacgtcg ccctggatga
    11401 actcggcctc ctccggcagc catccgggcc ggccgtacgc gtgcgtctgc ggctcaaggc
    11461 tgtcgagcac gcgcactttc cagccctcgg cgagtgcggc atcgacgatg tgcgagccga
    11521 ttaggcccgc gccgccagtg atcaatgctg tcgtcgtttc tgccataccg ggttctccgc
    11581 tatcggatca gaacaagcaa cgcgttatac catgaacgcc cttggcccac tggatgaacc
    11641 agatcatcgc atcgcctcga acgccccgtt caggtcagcg atcaggtctt cgggatcctc
    11701 cagcccgacc gagagccgaa gcctcgtgcc gacaccggcc gtctccgggt cgccgtccgc
    11761 gaggatgcgt ggcgggtaac agacggtcgt gatctcgccg ccgaggctgg tcacgtacgg
    11821 gatcatcctc agagaccgca caaaggctgc cgccggggcc tgtgtggctg tgccgttgaa
    11881 ctcgaccacc atcaggccgc tgcgcccgtc cgggagcatc cgccgcaccc gctccccctc
    11941 tgcgccggtc gcgagaccgg ggtagcgaac acaggccacc tccggctgct cggcgagata
    12001 ccgggcgata cggtcggcgc tctcgctgat ccatgccatg cgcggggcga gggtatggat
    12061 gccccgaagc gccagcgcgg aatcgtgcgc accgggaacg gccccgagaa ggtagccgac
    12121 ccgcgcgatt cgatcaatta ggaagcgtga gccgacaatc actccagcgg agaggtcgtg
    12181 atggccgccg aggaacttgg tgacgctgtg gatgaccaga tcggcaccgt gctccagcgg
    12241 ccggcagagc atcggggtcg ccacggtgct gtcgacgacc agggtgactc cgcgctcccg
    12301 cgtgattcca gccaaggcac cgaggtccgg cacggtgatg ccggggttgg tgattgtctc
    12361 ggcgaagata agcgtcgtct gcggacggat cgcgttccgc acagccccga ggtcatccag
    12421 cggcacgacg ctcccgccga tcccgaagcg ggcaagctcc cgcgtgagga gctcctcgga
    12481 gtcgctgtat ccccccgcgc cgacaatgag atgatcgccc tgcccgagca gcgccagcac
    12541 gaccgtcgtg acggccgcca tgccggagga ggtcgcgtag cccgcctcgg cgccctccag
    12601 ctcggcaacg gcctcctcaa gccgtgccgc gttcgggaaa tgcgcgcggt ggtaggtcgg
    12661 ctcgttacgc ccgatgcgat tcacaagcgt atcgggatcg cgccccgcga gcgcggtgct
    12721 cgtgacgatg ggatgcacca ccggccaggt gtcaccgtca ccgttgtggt ggccggtccg
    12781 agcggcgcgg gttcgcagcg atggcccggg atcgcgaggt gtcatgaaaa ctcctgtcac
    12841 tctggcgtgg cggacatcgg gctgccggcc ggcgtggcgg ctgtcgtcgc cctaggtgtc
    12901 gccaccacca cggtactgtc tggcggtgtc tgggagccgg ggccgggcaa caatctgcct
    12961 cccgactgct cctccccggc ccagcgaatc gggtagggca ggaaccagcg acggagcagg
    13021 tacacatgca ccgggtcgtc atccagccac tgaccgtact cacgctcctc cgccaccacg
    13081 tagccacgcc ggacggcggg atcagtggcg acgatgtagc tggagtcggt tggcttggcg
    13141 tagaagtaga tccaccacgg cgagcccgcc ggggatctcg cctcggtgat gttctcatcg
    13201 agcatgacgg tatagaggtg gtagccatcc caaccggagc cggcatcgag ccggtcgata
    13261 ggtacgccgt tctcgttggc agtctcggcc atgtcccaga tcgcgtccat gaagacgagg
    13321 tagtcgcgca caccagccac ggagacgacc gctaccgcgg ccaaggccag ccaggcgatc
    13381 ggctggaaga gatcgacatc ccgaagcgcg tagaagacga gcgggatcag gacggcgatc
    13441 accggcagca ggtagcggtc gagcgtcacg cccctgttag tgtagtgata ggacggtggg
    13501 aagacaccga tgagctgcca gagcccgacc gaggcgacga gccagaatcc ccagcgctcc
    13561 ggcaaccgcc cggcgaagag tttgcgcagc aacaccatgc cgaggagcag cgcgccgatc
    13621 gcggagacga tagtgagggc cacggcgaag tcggagccgc gctcccacag gcgctcgcgg
    13681 ctgccgagca cgtccggcgg tcccagaccg cctgggccga cgaactgcgg cgcgaagggc
    13741 atcgcctggc cgcgggtccc cgccaggtag agccccgcta ggatcagcgc cgaccacccg
    13801 gcgaaggcgt agaagccacc gggggtcgga aagagcgagg tgctataccg atggagtcgc
    13861 catggccgga ttgccagcag tacgggcacc agcagcagtg cgccgtagaa gatcgcgtag
    13921 agtgggatac gccggaccag gagccaggtt gcctcccatc ccttgtcgaa gatgtcctgc
    13981 aggaagtcct cctgcacgtc ggggacgtca ttaaaccagc gcagccaggc gtagtaggca
    14041 agcgccatca gcagcggcag cagcgccacg gcgagcaacc ggcggatgtc tcgccggcga
    14101 aaccagagcc ggccgctgag aaagagccac attgcgaccg cgagcgggat cagcgcgccc
    14161 tgttggcgga tcagaaacgc gaaccccgca aagaacgccc cggccagcac gaagccgagg
    14221 tgctcgcgtt ccggctggag cccgcgcagg tagaaggcga gggagatcag catcaccgag
    14281 gcgaggtggg cgtcggtcat gaagctgaat gccagtgcgt aggtcagcgg gttgaagagc
    14341 cacacggcca tgccgagcgc gctgcggctc cgggtgacgc cgagctggcg aaggatcgcg
    14401 tagagcgcga taccgccgag cgcgaccatc accacggtcg agacacgcat catccccagg
    14461 gacatgccga agatgatgcc gaagagcgcg ccccagagta cctgcccgac ggcggtcgcg
    14521 gcgaccaccg gaaagacggt caggcgtccc tcgtcgagca ggatctccac cgatcgggtg
    14581 taagcccagt catcggtggt ggcgatctgt gtcatcgttg ggacgaccat cgccccggcg
    14641 gcgaagagcg tcagtaccac ggcgaatggc cagttgtgca gcacggtctg gaccgcgtcc
    14701 cgtgcctgcc gggcgcggga tggtggtgga ggggacggct gcggaacgat cggggcggtc
    14761 acttcagttt cgtccggtga aggcgcttcg ggctcgcgcg cgattggcgg ggttggccga
    14821 aacagcccct cgccgttctc cgacgacggg cgctggatcc cgggttcggc gtctggacgc
    14881 ctctcggtca ccgctccctc gctctcgatc cgggtcgcac atgaccccag tgtgacttcg
    14941 gccaccaaga ccggtccggc gcacgctcgc aacgcgacca cgctcggata cagtaccatc
    15001 gactgaccat cccgattcgg cgttcgtcca atcgaaagga cgcgaacgtg gtccggagcg
    15061 cgccgcgccc atatccgcct gcactcgcac cgacgcgggc catcgccacc gtgcttccgc
    15121 ctccgacgct ggcggactgg gaccggtgcc gcgacgtgac ccgtatccac ggccgcacct
    15181 tctacttcgc ctgccagctg ctgccactca ccgagcgccg ctgcatccat gccatctacg
    15241 catggtgccg ccacgcagac gacatcgtcg acaccgccgc gccgggtggt atcgcagccg
    15301 cgcacgagca gctggaccga tggctcgcga ccatcgaccg gccggtcgac ccgatctccc
    15361 atgccttcgc ttgggtgatc cgcgagcacg gcgtccccac cgaacccgca caggacctga
    15421 tcgccggggt tcgaatggac cttgaacggg cgtccttcga gacatgggac gatctgcgct
    15481 ggtacagcta ctgcgtcgcg ggcacggtag ggctgatgac tgtgccgatc ctgggctgca
    15541 tcgatcgatc agcgctgccg cacgcggtca atctcggcat tgcgatgcag ctgacgaaca
    15601 tcttgcgcga cgtggccgag gacgccgaga tgggacgggt ctaccttccg gacgaggact
    15661 tggcgcgctt tggcgtctcg cgggagtcac tgctggccat gaacccggac ggcgattttc
    15721 gcgggctgat gcggttcgag atcgagcggg cacgcgggct ctaccggagc gcgcacaccg
    15781 gcatcccggc gctgtcgcgg gccgggcaga tcacaacgat cgccggcagc catctctacc
    15841 gggagatcct ccaccggatc gaagagcgcg gctacgacgt cttcgatggc cgagcgacgg
    15901 tcagcacccg gcgcaagatg cggaagatgc cgttggttgt cgcggccttc ctgcgcatgc
    15961 aactctccct cgtcggtacc cgcccccggg cgtgacaccc cgacagtggg tgggacacgt
    16021 caatctccat cgatccatgc agcttgcgag caacgacagg cgagtgcaat ccgtagcacc
    16081 ggcctcgcgc cggctcgacg gaacaacaca tccgcattta tcggacaggc tggggctgcc
    16141 cctaccgcga tggcgcagct cctgatgtcg atcactggtt gacggagatc aacaacccgc
    16201 ccatttgcag ggcgggtcta ccgactgaca ttctgttcgc tggccacacg ggttcgagcc
    16261 gggacgggga taccactgag cgcggcgacc tggctctcca gcacgcgcag cagctccgtg
    16321 tcagattcca gtccctcccg ggagagcggt gggccgatgc gcatggcgat ctcccagcga
    16381 ccgtcatcac gctgctcgta ggcaaacccg accggcacga gcgggatctc ccggccgagc
    16441 cgccgttcgg cgatgcgcac cagccgcacg acgcccggct ggaagggcac caccccttcc
    16501 gggctcgctc tctgcgtttt cgcatgcgga tcgacaaccg ggtagccctc aggaaacatc
    16561 accaccacct tgccggcggc cagcagatcc acaccgtcgc gggtcgccgc acgcagaagc
    16621 cgtcgtgcct ccgacttccg cttgggagcg gcagcctcgc ggtcgagtgc gtctgggcgc
    16681 aggatgatcg gccaggcgac catgcgggag agcttctcca tcacgcggcg gagtagccca
    16741 gtcccggccc agtcgagtcc gacgaggatg tgcagcgacc gtccggtcgc cgcgtagagg
    16801 gcacatccgt cgagcaggtt gtggtagtgc cgcgcgacga tcacgcaggg accggatgcc
    16861 gggatgtgct cggcgccagc gatgtcaagc gtcgcctgcc gatcgaggac gacccgtcct
    16921 ccccgacgga gtacctgctg cccgatctcg taggccggcc cggatgccgt catgccgccc
    16981 cgcggctgcc ccgcagcgcg aagagcgacg gcaggaggcc gagcatgacc gcgagcagga
    17041 ttggcaccca gaggtcgacg ctggcgctca gcaccgacgc gaagacgatg ttggcggcga
    17101 agacggcgac cgggaaccag atcgcgaggc gatgcccgcc acccgatggg tcggcccgcc
    17161 agagcaggcg cgcgacggtc atgaagacaa cggccgtgag cgtccagccg atgaaattct
    17221 gtgtcggcat gccgaagtac gggcccgtct cgtcccaggc ccagaacttg accgagagcg
    17281 actcgtgtgc catggcgggg tcgagcacga ggtcccagac ggtgagcagc caggcgccga
    17341 agatgacggc gtagatcgct cgtggccgaa ggttccactg tgcggcgagc acgtgaccga
    17401 tcatgtacgt cgcgaagccg acgtagaacc acgacagcgg gatcgagaag ggtacgtggt
    17461 cgagcacctt gtacccgagg aagttggtgt acgcgtagtt gccgaagggc tcaccggtgc
    17521 tggtgccgag cagctcggag gtgagcgaga tgggcaccgc ggcagcgaag aagatggccg
    17581 tgcggcggat gccgattgcc gcgatgcccc agaagaacat cgccgtcgca ccgaagatga
    17641 tgtgcaggga cccggcgtag cgcattccga agtcgtagac gcgcaggcct acctcggaat
    17701 cggcccacag gtcagggttg gggatcgcga tcagcatccc ggcaagcccg aagacgagcg
    17761 ccgcgagatg gccgatcagc aacgccttcg cgatctgcag gggccggcgg tcaccgagcc
    17821 acgagacgct cttgtgggcg gcgtgccggg tgaccgtatc gacggttgcc tgctgcatcg
    17881 tttgtgtcct ccgggtggtg cctaccaccg aaccactctg ccgcgccacg tgtgctcgcg
    17941 ccggcgcgcc tgccccacga gtatcgtcac ggcaaccgga tccagcagca ccgcgagcca
    18001 gtgggtagcg ggcaatgcca cgtaggcgcg ggccatgccc gcctgcgtgc cgattcgcgt
    18061 cgccagcagc ccggcattga gccggatcgc aagcgagcgg agcggcatgc gccgccagat
    18121 tgctgccagt atcagcatgg gcaccggcac gcccatcgcg aatgtcaggt ccgccatgcg
    18181 agacgcccac ggcatccctg agtcgcggtc ccgcatcggc aacgatcgtg accagctgct
    18241 gagggcatcg taccagtccg cgtacatctc gacagtgacc agcgaggcat cacacgctgg
    18301 ctcgaagaac ccgtaccgga tgccgacgcg cacacagcgc cgcgcgagcg tgacatcctc
    18361 caccagcgac tgcgccacgg cggcgaaccc gccggcggtg tcgagcgcgg atcgctcgat
    18421 cagcaagcac tgcccgttgg cctgcaccgc gtcagggtcc tcgaagacct ggccggggat
    18481 gccgtagcgg tagacgagtg ttgccagcag tgaggtatgg accggcgctt cgatctgtcc
    18541 ggagacccgc tggggtgtcg ccacactcag tgcgccgaga gcgcgccgct cagcatgcgc
    18601 gaggagcgat gtcgccagca tcggctccgg gcggacatcg gcgtcgatcg tgagcagcca
    18661 gcgcgaccca agatcggccg agatcgcacc ggtatgcagc ccccatggct tgccgttcca
    18721 accgtccgga accggactgg cgttgatgaa acggatgcgc gggtcacgaa cggtccaggc
    18781 ctccgccagc gcgcgcgtcc tgtcggtcga accgccatcg acgacgagga tctcgcgtac
    18841 ggcttcgccc tgtcggctga gcccggcgag gcacggctcc aggcggtgct cctcgttcag
    18901 caccggcacg atcacgctca cggagccatc atcggg
//
LOCUS       DASYWL010000180         1546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_627624, whole genome shotgun sequence.
ACCESSION   DASYWL010000180 DASYWL010000000
VERSION     DASYWL010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627624"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="VGR22_06425"
     CDS             <1..1101
                     /locus_tag="VGR22_06425"
                     /inference="COORDINATES: protein motif:HMM:NF016952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HEV2128235.1"
                     /translation="LDFTLDEPIDAFTVEAIETVVQEWAACTNAGDQLRAYTLLTDDY
                     AQQGPVGASRDEARTMLATSPVPVSPEAAVSVGSAHDARVFPDGQAGATFATIGVRDP
                     GEALTVFMLFEQPDGRWRIDGMTGVVAPAPTAPVWDYRVVAEYPHDTGAYTQGLVISD
                     GQLFEGTGQWEESELRRVDLETGEVLQSHPLDPELFGEGIVVLADRIYQLTWQAGICF
                     VYDRETFELLETFTYSGEGWGLTTDGERLIMSDGGSRLVFRDPDTFAVLNSVIVQDGG
                     LPVSYLNELEYIDGEVWANIWQTDHIVHIDPATGNVTGWIDLTGLLPSDYPRAEDAEV
                     LNGIAYDSETGRVFVTGKYWPAVFEIELVPPE"
     gene            1264..>1546
                     /locus_tag="VGR22_06430"
     CDS             1264..>1546
                     /locus_tag="VGR22_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128236.1"
                     /translation="MSAFPFTDQSRIRRRHGLSFPLLLSLAVASALTGAVVALVVQRL
                     LQGAQYTEVLPRPASVRHLQESSAPSESSETAGEASVAPDLAERAELATA"
BASE COUNT          256 a          514 c          505 g          271 t
ORIGIN      
        1 ctggacttca cgctggacga gccgatcgat gccttcacag tggaggcaat cgagaccgtt
       61 gtccaggagt gggcggcatg caccaacgcc ggtgaccaac tgcgcgctta caccttgctc
      121 accgacgact acgcgcagca aggcccggtt ggcgcatctc gcgacgaggc ccgcaccatg
      181 ctggcgacgt cacctgtccc cgtttcgccg gaggctgcgg tgtcggtcgg atcggcgcac
      241 gacgctcgcg tgttcccgga tggtcaggcc ggtgccacct tcgccacgat cggcgtcagg
      301 gatccgggcg aggctctgac ggtattcatg ctgttcgaac agccggatgg ccggtggagg
      361 atcgacggga tgaccggagt cgtggcgccc gccccgaccg caccggtctg ggactatcgc
      421 gttgtggcgg agtatccgca cgataccggc gcctacacgc aagggctggt gatcagcgac
      481 ggccaactct tcgaaggaac tggccagtgg gaggagtcgg agctccgacg ggtggatctt
      541 gagacgggcg aggtgctgca atcgcacccg ctcgacccgg agctcttcgg cgagggcatc
      601 gtcgtgctcg ccgatcgtat ctaccaactc acgtggcagg ccggcatctg cttcgtatac
      661 gaccgcgaga catttgagct gctggagacg ttcacctact ccggcgaggg ctggggcctc
      721 acgacggacg gcgagcgcct gatcatgagt gacggcggca gcagactcgt tttccgcgac
      781 cccgatacgt tcgcagtgct caactccgtc atcgtgcagg acggcggctt gccggtgtcg
      841 tatctcaacg agctggagta catcgatggt gaggtctggg cgaacatctg gcagaccgac
      901 catatcgtgc atatcgatcc cgccaccggc aatgtcaccg gctggattga cctgaccggc
      961 ctgcttccct ccgactatcc acgggcagag gacgccgaag tcctgaacgg catcgcctac
     1021 gattcggaaa ccggccgggt gttcgttacc ggcaagtact ggccggcagt gttcgagatc
     1081 gaattggtcc cgcccgagta gccgcttggt ccctcccggc ggcccgcacg acgattcgca
     1141 atccccccat ggcgtgccca gcgggtcccg ggataccact cgtgccgcca aggctgggcc
     1201 agaccgggat cttgccgtag aatcctggca tcgaccccga tcacggcaag gagcaccagc
     1261 gcgatgtcgg ctttcccctt taccgatcag agccggatcc gtcggcgcca cggactctcc
     1321 ttccctcttc tcctctccct tgccgtcgcc agcgcgctga ccggcgcggt ggtggcgctg
     1381 gtggtccagc gtcttctcca aggtgcccag tacaccgagg tgctgccgcg gccagcgtcg
     1441 gtgcgccatc tgcaggagtc gtccgcgcct tccgagagca gcgagactgc cggcgaggcc
     1501 agcgtcgcgc cggacctcgc agagcgagcg gagctggcca cggcgg
//
LOCUS       DASYWL010000181         2527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_139440, whole genome shotgun sequence.
ACCESSION   DASYWL010000181 DASYWL010000000
VERSION     DASYWL010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139440"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VGR22_06435"
     CDS             complement(<1..344)
                     /locus_tag="VGR22_06435"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV2128237.1"
                     /translation="MKITRSSLETMIGPTDWFTGNVYVDSIAAPTEASRVSAASVHFS
                     PGARTAWHTHPNGQTIFITEGVGRAQRRNGPVEVIRPGDRVYFEPGEEHWHGAAPDRF
                     MAHIAIQEVNDEG"
     gene            complement(399..647)
                     /locus_tag="VGR22_06440"
     CDS             complement(399..647)
                     /locus_tag="VGR22_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128238.1"
                     /translation="MTVPDYIISRTIAVEHQQRQSRHTSYDDLPEPRPRSKAMNEAFP
                     ALRRQIGFLLRVAADRIEPEPTSRHRSHRVTTHLQVLE"
     gene            complement(807..1499)
                     /locus_tag="VGR22_06445"
     CDS             complement(807..1499)
                     /locus_tag="VGR22_06445"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV2128239.1"
                     /translation="MPHRLVDQGLRIDSGRHERCSLPTTRLFPETGGNALCTQRLCMA
                     RVMSASRPSPTPASSSRPTPSSASLAPRSAAAETHGWTRFVSMQNHLNLIYREEEREM
                     LPLCREEGIGVTPYSPCASGRLTREWSSESTLRSETDQIAKSKYDVTEDADRQVVERV
                     TKVAENHGVPRVHIALACLLQKEPVSAPIVGATKITHLESAAGALAVAVTQDEVACLE
                     EPYMPHPVVGPV"
     gene            complement(1518..1718)
                     /locus_tag="VGR22_06450"
                     /pseudo
     CDS             complement(1518..1718)
                     /locus_tag="VGR22_06450"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
     gene            2077..>2527
                     /locus_tag="VGR22_06455"
     CDS             2077..>2527
                     /locus_tag="VGR22_06455"
                     /inference="COORDINATES: protein motif:HMM:NF018431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV2128240.1"
                     /translation="MDVVSRPEVGQEGSATPALHQELVERVARSVPENGTAEPLEGLV
                     LRRATAPTEVGHGLAHPSFCVIAQGSKEVRLGDRRYRYDPAHYLIATTAVPVASRITE
                     ASPERPYLGIVLRLDPALVASVMVEAGRPAPRGQSEMAIDVSPLDAGL"
BASE COUNT          399 a          781 c          847 g          500 t
ORIGIN      
        1 ccctcgtcgt tgacttcctg tatcgcgata tgtgccatga atcggtccgg ggcggcgccg
       61 tgccagtgtt cctcgccggg ctcgaaatag acccggtcgc ccgggcggat gacctcgacc
      121 ggcccattgc gccgctgcgc acggccgacg ccctcagtga tgaagatcgt ctgcccgttg
      181 gggtgggtat gccacgccgt gcgggcgcca ggtgagaagt gcacactagc ggcgctcacg
      241 cgcgatgcct cggtcggggc tgcgatcgag tcgacgtaga cgttgcccgt gaaccagtca
      301 gttggcccga tcatggtttc gagcgagctc ctggtgatct tcatgtcgtc tcctccagtc
      361 ttgtgggtgc ttcgccctcg ggtgtgatcg tcgccgtgct actccagcac ctgcaaatgt
      421 gttgtgacgc gatgcgaccg gtgacggctc gtgggctccg gctcgatgcg gtcggcggcg
      481 acgcggagca agaaacctat ctgccgccgc agagcgggaa aggcctcgtt catcgccttg
      541 ctccgaggac gcggttctgg caggtcgtcg tacgacgtgt gccgactctg gcgctgctgg
      601 tgctccacgg caatggttcg gctgatgatg taatcgggta cagtcattgt tgtcctcttc
      661 gttgtttgtc agtgtgatcg gggcgtggcc gtgtctcgca tcgtcgcaga gggcccgggc
      721 gatatggcga tcaggcacag tgggcgcagg gatgtccgct acttacggcc ccgctcgtcg
      781 gatcacgcat gctgtggggg gccgtcctac acggggccga cgacgggatg aggcatgtag
      841 ggctcctcca agcaggcgac ctcgtcctga gtcaccgcga ctgccagcgc gccggcggca
      901 ctttccaggt gtgtgatctt cgttgccccg acaatcggcg ccgaaactgg ctccttctgt
      961 agcaggcagg caagcgcgat atgcacccgc ggtactccat ggttttccgc gaccttcgtg
     1021 actcgttcca caacctggcg atcagcgtcc tccgtcacgt cgtacttgct cttcgcgatc
     1081 tggtcggttt cggaacgaag cgtgctctcc gatgaccatt cacgggtcaa tcttccggag
     1141 gcgcaggggc tgtatggtgt gaccccaatc ccttcctcgc ggcagagcgg cagcatctcc
     1201 cgctcctcct cgcggtagat caggttcagg tggttctgca tggagacgaa gcgggtccac
     1261 ccgtgagttt ccgctgccgc agaccgcggc gcgagtgacg cggatgaggg tgtcggtcgg
     1321 ctcgatgagg cgggcgtcgg ggacggtctc gatgcggaca tcacccgcgc catacataac
     1381 cgttgcgtgc atagtgcgtt tcctccggtt tcagggaata ggcgagtcgt tgggagcgag
     1441 catcgttcgt gcctgccgct gtcgatgcgg agtccctggt caaccaggcg atgcggcatg
     1501 caccgttgca cgccgggtta ctcaccacgg aacacctgct tcgccacacc catcgcggcc
     1561 atcgccttcg gccagccggc gtagaaggcg agatgggtga tgacctcgat gagttcctct
     1621 tcggtaaggc cgttctgttt ggcgaggtcc aagtgcccta caagctgctc ggtgttgccg
     1681 ctggacacga gggaggcaac cgtcacgagg ctccgctccc gaggcgagag ctacgggcgc
     1741 gtccagacct ggtcaaaaag gacgtcatcg gtgaagccca ccagggcggg cgcgaaatcg
     1801 ccgaacatct gctcggcacg cgagggtccc tgtttgtcag ccatgtcttg cctcctccac
     1861 ggtcgtggca ctgccggcac atatccccgt ggcccgcccg taattggatt gttattgacg
     1921 attcgtccga attgccggaa ctctggtgtc aacgcatccg tgtctggtcg atagtttatc
     1981 cctggggtgg aggtgcctgg taggaagatc gtccgcgatt gttgtatgat cctgcagagc
     2041 gcatggaagc acggaccaaa accgcgagag gcagctatgg atgtggtaag ccgcccggag
     2101 gtcgggcaag agggtagcgc gacgccagcc cttcaccagg agttggttga gcgggtcgcg
     2161 cggtccgtgc ccgagaacgg gacagcggag ccgttggagg gactggtgtt gcgccgcgcc
     2221 accgcaccga cggaagtcgg gcatgggctg gcccacccgt ccttctgcgt gatcgcgcag
     2281 ggaagcaagg aggtccggct cggcgaccgc cggtaccgct acgatccggc acactacctc
     2341 atcgcgacca ccgcggtacc cgtcgcaagt cggatcaccg aggcgtcacc ggagcggccg
     2401 tatctcggga tcgtgctccg gctggacccg gcgctcgtcg cctcagtgat ggtcgaggct
     2461 ggccgtcccg ccccgcgagg gcagtccgag atggcgatcg acgtgagccc gctggacgct
     2521 ggcctgc
//
LOCUS       DASYWL010000182         5981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_215132, whole genome shotgun sequence.
ACCESSION   DASYWL010000182 DASYWL010000000
VERSION     DASYWL010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215132"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VGR22_06460"
     CDS             <1..331
                     /locus_tag="VGR22_06460"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV2128241.1"
                     /translation="IAGDRWQGEAWGLVFCSTIGTPLDPSNVTKQYRALLQAAGLEQR
                     RFHDLRHSTGSFLASRGVHPRIIMEILGHSQVSTTMNTYTHVELGSMRDALDELGDLL
                     DADKASS"
     gene            complement(432..503)
                     /locus_tag="VGR22_06465"
     tRNA            complement(432..503)
                     /locus_tag="VGR22_06465"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(469..471),aa:Thr,seq:tgt)
     gene            complement(603..2948)
                     /locus_tag="VGR22_06470"
     CDS             complement(603..2948)
                     /locus_tag="VGR22_06470"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEV2128242.1"
                     /translation="MSMLQPDDGANHLPVPRTSLVGREEDAERVEDLLLSPDVSLVTI
                     VGPGGAGKTRLALKVAEDLENAFRDGVWFAPLSTISDPKLVSPTIAQAIGMRERSGDS
                     VEEQLRLHLRDAEMLIVLDNFEQVRESAVQIARLLQACRGLKILVTSRVILRLSAEHV
                     YPISGLRLPGAEEAHDVAGIVHNEAVRLFVARAQSSDPAFRLDTHNAEAVVGICRQLD
                     GLPLGIELAAARVRTLSPQALHARLSRRLTVLGSRFQDVPDRHRTIGTAVDWSVQLLT
                     PAEQVIFHRLSVFSGSFSLAAAEVVAGVELGTGTSDLDVLDALGSLVDQSLLRHVSES
                     GDEESRFRMLRVVREVALENLEASGELEDARRRHAEYFLALAEEAAPHLTGPEQVRWL
                     NELERSLADLRAAMTWFGQAGEHALRLRLVAALWRFGYTRGHLTEAREWLRTALLASP
                     EKTQTRAVALNGKGLLASSQGHSAVATACHEEALELSREAGDEYAIAVALNGLGDAAA
                     VDGDRTLARERYEGALQLFRKVGHERGVAGAHTNLGNLAWDAREYEEAVAHHQQAAVH
                     YEAAGDLRGTAWSASNLGTLAVQQGQYGAATRYLRDAMGKYTELGDPVGVMETLEGFA
                     GIALARKQASLATTLYAVAQVIRERIGIPIAAADRERHKALVERMRGELGASFDMTWA
                     AGRAMSGEEAMRLALATDSGAEPSSPQRGAATTADSFRLTRREIEILQLIAVGKTNKE
                     ISDELSISYRTVSTHVSNVLGKLDLANRSALASFAHREGLV"
     gene            complement(3207..4286)
                     /locus_tag="VGR22_06475"
     CDS             complement(3207..4286)
                     /locus_tag="VGR22_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEV2128243.1"
                     /translation="MATYQITLIPGDGIGPEVADATRLVLDATGIAFEWDVQQAGSDA
                     LDQTGELLPQATLDSIRRNGVGLKGPITTPIGSGFRSVNVGIRQELELYANLRPGRTI
                     KGVQSIFENIDLVVVRENLEDLYAGVEFDDGSWEAGEVIEAINARSNKRISPSAAISV
                     KMITPEGSRRIVEYAFQYAQKNGRKKVTAVHKANIMKFSDGLFLEVAQDVAKNYPDIE
                     FNDRIVDNMCMQLMQKPDQYDVLVMPNLYGDIVSDLVAGMVGGLGVAPGGNVGKDAAV
                     FEPIHGSAPSHAGKNEANPTAMLLSGALMLRHLGEVEAAQRVENAVEAVISKGETVTY
                     DLRADRDPSKAASTTEMAEAIIAAL"
     gene            4410..5978
                     /gene="gpmI"
                     /locus_tag="VGR22_06480"
     CDS             4410..5978
                     /gene="gpmI"
                     /locus_tag="VGR22_06480"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEV2128244.1"
                     /translation="MMHESSYPVVLVILDGWGLGEPVESNAVWSADTPVMDGLVAAYP
                     NATLSTSGTDVGLPEGQMGNSEVGHLNIGAGHIVYQWITRIDRDIEDGTFAQNRVLRA
                     AFERAQSNGGAVHLMGLAGEGGVHAHSRHLTALIDMALEWRDVSVRVHPFTDGRDTAP
                     DSGRGFLLEIEDHIAASPVHDIQFASLSGRYYAMDRDRRWERTKRAFDAVTGTGGPNV
                     GSGLDAIEASYAKGITDEFIEPVRIGDWGESDNRMGPNDEAIFFNFRNDRARQLTQAL
                     TTPDFDAFDRSAVPNLPMSMTTMTRYEEGLPVHIAYAPTDIPNPIAKVISDAGLRQFH
                     TAETEKYPHVTFFLNGGREEPFPGEDRKLVPSPKVATYDLQPEMSAPAVCEGVLEAIW
                     GGEYHFIIVNFANPDMVGHTGIILAARKAVETVDACLGEIMAAVQTAGGIALVSADHG
                     NADVMRVPGTDEPMTAHTTNPVPVVLVAPDDSPLRHATLRTNGRLSTLGTTVIDLLGL
                     PPHPDMTEPSLLAQ"
BASE COUNT         1085 a         2016 c         1837 g         1043 t
ORIGIN      
        1 gattgccggt gaccgctggc agggtgaggc gtgggggctg gtgttctgtt ccacaatcgg
       61 gacgccactc gacccgagca acgtcacgaa acaataccgg gcgctcttgc aggcagcagg
      121 gctcgagcaa cggcgcttcc atgacctgcg acactcgacc ggctctttcc tggcgtcgcg
      181 cggggtacac ccgcggatca tcatggagat cctcggtcac tcgcaggtca gcacgaccat
      241 gaacacctac acgcatgtgg agctcggctc catgcgcgat gccctggacg aactcggaga
      301 cctcctcgac gccgataaag cgtcaagctg aggcgatctg ctgcaggata tgctgcagcc
      361 ggaggcagct gcgatgcccg cgactctggc accacagaca gaatccccgg aatatcaaca
      421 aaactggacg gagccgacgg agggattcga acccacaacc tactgtttac aaaacagttg
      481 ctctgccgtt gagctacgtc ggcatgcgaa acaccagccg ggctgccgac cgaacgcctc
      541 ggaagtatag ggatcgcgcc ggtgaagcgt caagacgtca cgcgagtggg tggacacgtg
      601 ggtcacacca gtccctcacg gtgcgcgaac gacgccagcg ccgaccggtt tgccaggtcc
      661 agcttgccca aaacattgga gacatgcgtc gacaccgtgc ggtagctgat gctcagctcg
      721 tccgaaatct ccttgttggt ttttccgacg gcaatcagct gcaggatctc gatttcgcga
      781 cgcgtgagac ggaacgaatc agcagtcgta gcagccccgc gttgtggcga ggaaggttct
      841 gctccgctgt cggttgccaa ggcaagccgc atggcctcct cgccgctcat cgccctcccg
      901 gccgcccagg tcatatcgaa cgaagcgcct agctctcccc gcatccgttc aaccagcgcc
      961 ttatgccgct cacgatccgc ggcagcgatg gggataccga tccgctcacg aatgacttgc
     1021 gcgaccgcat agagggttgt cgcgaggctg gcctgcttgc gagctagcgc aatccccgcg
     1081 aagccctcga gcgtctccat gaccccaacc gggtcgccga gctccgtgta cttgcccatg
     1141 gcgtcacgca gatagcgtgt cgccgctccg tactgaccct gctggacggc aagcgtgccg
     1201 agattcgacg ccgaccatgc cgtcccgcgc agatcgccag ccgcctcata gtgcaccgcc
     1261 gcctgctggt gatgggcaac agcctcctcg tactcccgcg catcccaggc taggttgcca
     1321 agattggtgt gggccccggc gacgccgcgc tcgtgcccaa ccttgcggaa cagctgaagg
     1381 gcgccttcgt accgctcgcg agcgagggtc cggtcaccat cgaccgccgc cgcatccccg
     1441 aggccgttga gggcgacggc aatcgcgtac tcgtcgccgg cttccctgct caactccagc
     1501 gcttcctcgt ggcaggcagt tgccacggcc gaatgacctt gggagctggc cagcaacccc
     1561 ttcccgttga gtgccacagc tcgagtctgg gtcttttcag gactggccag cagcgctgtc
     1621 cgcagccact cgcgcgcctc agtgaggtgg cctcgcgtgt agccgaaccg ccagagcgcg
     1681 gcgacgagcc gaaggcgcaa cgcgtgctct cccgcctgcc cgaaccaggt catcgccgcc
     1741 cgcagatcgg cgagtgaacg ctccagctcg ttgagccatc gaacctgttc cggtcccgtc
     1801 agatgcggcg cagcttcctc ggcgagcgcc agaaagtatt cggcgtggcg gcgcctcgcg
     1861 tcttccagct cgccgctcgc ctcgaggttc tccagtgcaa cctcgcgaac caccctgagc
     1921 atgcgaaacc gcgactcctc atcgccggac tccgagacgt gacgcagcag gctctggtcg
     1981 acgagggatc caagagcatc cagcacatcg aggtcggatg tccccgtacc cagctccact
     2041 ccggcaacga cctcggccgc tgcgagcgaa aagctgccag agaaaaccga aagccggtga
     2101 aagatcacct gttcggccgg tgtcagcagc tgcacgctcc agtcgaccgc agtgccgatc
     2161 gtgcgatgtc ggtccggcac gtcctggaac cggctgccaa ggaccgtcag ccggcgagat
     2221 aacctcgcat ggagtgcttg cggcgagagg gtgcgcacgc gggcagcggc cagttcgatg
     2281 ccgagaggaa gtccgtcgag ctggcggcaa atcccgacca cggcttccgc gttgtgagtg
     2341 tcgagacgga acgccgggtc gctcgactgg gcccgcgcaa cgaagagacg gacggcctcg
     2401 ttgtgtacga ttccggcaac gtcgtgcgcc tcctccgcac cgggcaagcg caggccggag
     2461 atcggataga catgctcggc ggagagcctg agaatgactc tgctggtcac gaggatcttc
     2521 agaccgcggc aggcctgcag cagccgcgcg atctggacag cggattcgcg gacctgctcg
     2581 aagttgtcca gcacgatcag catctccgcg tcgcggagat ggagtcgcaa ctgctcctcg
     2641 accgagtcgc cactcctctc tcgcatgccg atcgcctggg cgatggttgg cgaaaccagc
     2701 ttcgggtcgc tgatcgtact gagcggagcg aaccacacgc cgtcgcgaaa cgcgttttcc
     2761 aggtcctccg ccaccttgag cgccaagcgg gtcttgccgg ctccgcctgg cccgacaatg
     2821 gtcaccagcg agacatccgg cgagaggagg agatcctcca cccgctcagc gtcctcctcg
     2881 cggccgacga gggacgtgcg cggcaccggc aaatgatttg cgccgtcatc gggctgcaac
     2941 atgctcatcg gtgtttcctt aagtctttcc cagattcctg ttcctagact tagcctccgg
     3001 attggccgct gatgctctgt aatgtacatc actaccggcc gcgtcggata tgcctgggct
     3061 gtcaggacac gccacaccgt ggctcgcgtc gcaacggtta cctgcgtctg gttgctacga
     3121 cgctcttccc ctgcgggaca gaaacaccgg ccgccggtga agcagcggcc ggtgccagtg
     3181 tgcttgcgcg acgtgccaag ccgggactag agcgccgcga tgatcgcctc ggccatctct
     3241 gtcgtgctcg ccgcctttga cgggtcgcgg tcggcgcgga ggtcataggt caccgtttcg
     3301 cccttcgata tcaccgcctc gacggcattc tccacacgct gggcggcttc gacctcgccg
     3361 aggtggcgca gcatcagcgc gccggagagg agcatcgcag tcgggttcgc ctcgttcttg
     3421 ccagcatgcg acggtgcgga gccgtggatc ggctcgaaca ctgccgcgtc cttgccgacg
     3481 ttgccacccg gcgccacgcc gagcccacca accatgccgg cgacgagatc gctgacgatg
     3541 tcgccgtaga ggttcggcat caccagcacg tcgtactggt ccggcttctg catgagctgc
     3601 atgcacatgt tgtcgacgat tcggtcgttg aactcgatgt ccggatagtt cttcgccacg
     3661 tcctgtgcaa cctcaaggaa gaggccgtcg gagaacttca tgatgtttgc cttgtgaacc
     3721 gctgtcacct tcttgcggcc gttcttctgc gcgtactgga aggcatactc gacgatccgg
     3781 cgggaaccct ccggcgtgat catcttcacg ctgatcgccg cgctcggaga aatccgcttg
     3841 ttgctgcgcg cattgatcgc ctcgatcacc tcgcccgcct cccaagaccc atcgtcgaac
     3901 tcgacgccgg cgtagagatc ctcgaggttc tcgcggacga cgaccaggtc gatgttctcg
     3961 aagatggatt gcacgccctt gatcgtccgg cccggtcgaa gattggcgta gagttcgagc
     4021 tcctggcgga tgcctacatt cacgctccgg aagccactac cgatcggagt ggtgatcgga
     4081 cccttgaggc cgactccgtt ccggcggatc gagtccagcg tcgcctgcgg cagcagttcg
     4141 ccggtttgat cgagcgcgtc gctgcccgcc tgctgcacat cccactcgaa cgcaatgccc
     4201 gttgcgtcca gcaccaagcg ggtcgcgtcc gcgacctccg gaccgatgcc gtcgccgggg
     4261 atgagcgtga tctgatacgt cgccatgact ctcgtctctc ctcacggttc gctgcaacct
     4321 gctacggtga tattgtcgca tgacatgccg gagcaccgac tcccgaaatc ggcgctcagg
     4381 aaagcaccaa tgacaaggga cgattcaaca tgatgcacga gtccagttac ccggtggtgc
     4441 tggtgatcct cgatggctgg ggcctcggcg agccggtcga gagcaacgcc gtctggtccg
     4501 cagacacacc cgtgatggac ggcctcgttg ctgcctaccc aaacgctacc ctgagcacgt
     4561 cgggaacgga cgttgggctg ccagaagggc agatgggcaa ctcggaggtc ggccacctca
     4621 acatcggcgc cggccatatc gtctaccagt ggatcacacg gatcgatcgg gacatcgagg
     4681 acggaacctt cgcgcagaac cgtgtacttc gcgccgcctt cgagcgcgcc cagtctaacg
     4741 gcggagcagt gcacctgatg ggcctcgccg gcgagggagg tgtgcacgct cacagccgcc
     4801 atctcacggc gctgatcgac atggcgctcg agtggcgcga cgtgtccgtg cgcgtccatc
     4861 ccttcaccga cgggcgcgac actgcaccgg acagcggcag gggtttcttg ctggagatcg
     4921 aggaccacat cgccgccagc ccggtgcacg acattcaatt cgcttcgctc tctggccgct
     4981 actacgcgat ggaccgcgat cgccgctggg agcgcacaaa acgggcgttc gacgccgtca
     5041 ccggcaccgg cggcccgaac gtcggctcag gcctggatgc gatcgaagcc tcgtatgcga
     5101 aaggcatcac cgacgagttc atcgagccgg tgcggatcgg cgactggggc gagagtgaca
     5161 accgaatggg gccgaacgac gaggcgatct tcttcaactt cagaaacgac cgagcccggc
     5221 agctcacgca ggcgctgacc acgcccgact tcgacgcctt cgaccgcagc gccgtgccga
     5281 atctgccaat gtccatgacc accatgacgc gctacgagga gggattgccg gtccatatcg
     5341 catatgcgcc gacagatatc cccaacccca ttgcgaaggt gatcagcgac gccggtctgc
     5401 ggcagttcca caccgcggag acggagaagt acccgcatgt gaccttcttc ctcaatggcg
     5461 gtcgcgagga gccgttcccc ggtgaggatc gcaagctggt tccgtcgccg aaggtggcga
     5521 cgtacgacct gcaaccggag atgagcgcgc ccgccgtctg cgagggagtg ctggaggcga
     5581 tttggggcgg cgagtaccac ttcatcattg tgaacttcgc gaatcccgac atggtcgggc
     5641 acaccggaat catccttgcc gcccgcaagg ccgtcgagac ggtcgatgcc tgtctcgggg
     5701 agatcatggc ggccgtccag acggcaggcg ggatcgcgct cgtctcggcg gatcatggca
     5761 acgccgacgt catgcgagtg cctggtaccg acgagccgat gaccgcccac accaccaacc
     5821 cggttccggt cgtgctggtc gcgccggacg actctcccct gcgtcacgcg acccttcgca
     5881 ccaatggccg gctctcgaca ctcgggacaa cggtcatcga tctcctcggc ctcccccccc
     5941 acccggatat gaccgagccg tccctgctcg cacaatgagg g
//
LOCUS       DASYWL010000183         4085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_252649, whole genome shotgun sequence.
ACCESSION   DASYWL010000183 DASYWL010000000
VERSION     DASYWL010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252649"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            286..759
                     /locus_tag="VGR22_06485"
     CDS             286..759
                     /locus_tag="VGR22_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128245.1"
                     /translation="MKFGSERDRAAFLAASPARDVIAGGDDAVPVPMHVARERERGYN
                     QAALIAEYACRALSRETFRRALVQHLERPSQVGLNAQERRDNVRRSFSIAGDVVPSPR
                     KRYVLVDDVRTTGSTLAACVEALRPLRAARIDVVTFAAELRSEVIEATVKPFPDS"
     gene            complement(765..1904)
                     /gene="dxr"
                     /locus_tag="VGR22_06490"
     CDS             complement(765..1904)
                     /gene="dxr"
                     /locus_tag="VGR22_06490"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEV2128246.1"
                     /translation="MTTRIALLGSTGSIGRQTIDVIEQHPDTFKIVTIAAGRDTKLLR
                     EQVRAHRPTLVVSAERNAALDGIEWQPTPDGLVAAATHPDVDLVVFATSGHDAIPATI
                     EAIKQGKEIALANKEAIVCAGDLIMPLAREHGVTVRPIDSEHSAIWQSLASGTADTIE
                     RIILTASGGPFRLTPRSALASVTYEQALKHPNWSMGAKITVDSATLVNKGLELIEAHH
                     LFDTPFDRIDVVVHPEQIIHSLIEFIDGNSIAQLSFPDMRLPIQYALTWPERLAATAS
                     RPLDLVSIGSMTFEAPDLDRFPALRLAREAGEAGRTYPTVYSAVDEIAVRAFASGSIT
                     FPGIPRLLETVMARHPAHDVASLDDVVAADAWARREASNVVRAIV"
     gene            complement(1956..2822)
                     /locus_tag="VGR22_06495"
     CDS             complement(1956..2822)
                     /locus_tag="VGR22_06495"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEV2128247.1"
                     /translation="MRTRAVSSLGVIAVGVIAGVIGGPVFAAVMAAIGLGVYYETSRI
                     TPLIGLGYRLEWPGYVVIFLGASISLLSQEPWSFASFISIAVLLPTVMILQLPVSSES
                     FAAWITTAAVGAYVGIAVFAAIALRQEAGILDAEWANDVGTYFSVSDDSYALGMAWTA
                     IAIVVAWLSDTFAFLVGHTMGRTPLIPHISPKKTLEGAVGGVLGAVIGTVTLTLLLGI
                     PDVSVAQAIVIGVVLSIVGVGGDLSESFIKRCGGVKDSGTLIPGHGGIFDRVDALLPI
                     FLVTWVIVRILQ"
     gene            complement(2835..3617)
                     /locus_tag="VGR22_06500"
     CDS             complement(2835..3617)
                     /locus_tag="VGR22_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEV2128248.1"
                     /translation="MTAHPPRSVSPTPAAHESSSTPIDPSRVPRHVAIIMDGNGRWAR
                     QRGLARLEGHEAGTENIRRITHAAGQVGIRYLTLWAFSAENWRRPREEVEGLLNILSR
                     AIETETEELHRQGAQLRHIGDLRSLSPDLQGAIEGAIERTKYNDKLVLTLAFNYGGRQ
                     ELTRAVQNIVASGVAPEDIDEDMIERYLYTHDLPDPDLIIRTSGEYRLSNFLCWQGAY
                     SELHFSPTLWPDFSPGDLESAVRDYAQRERRFGAISNSSITG"
     gene            complement(3620..>4085)
                     /gene="frr"
                     /locus_tag="VGR22_06505"
     CDS             complement(3620..>4085)
                     /gene="frr"
                     /locus_tag="VGR22_06505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEV2128249.1"
                     /translation="ATPALLDRIHVDYYGAQTPLNQLAGISAPEPRLLVIQPWDRSSI
                     GPIERAIQSSDLGLTPNNDGQLIRISIPSLTEERRKQLVRIVHQTVEESKVAVRNIRR
                     DAVSHLRKQLTDKEISQDDERRAIDEIDALSKRFVDRAEEIGKEKEQEVLEV"
BASE COUNT          692 a         1217 c         1386 g          790 t
ORIGIN      
        1 gccgcctttt tgtacccggc ccggattcag cccgtggccg gatttcgctg ttcatacgtg
       61 acctgttcta tccgcgactg tgcagtggct gcggcgagcg ggggacgtgg ctctgtgagg
      121 tgtatgacgt ctgtaccgct cttggcggat gtcacgtcga cgtgtcggcg ttgcgggatt
      181 cccatcttac gtggaagatg cggatgtgga gacctgcacc cgcgggtgac gagggcggtc
      241 agggcttttc cctacaccgg acgggtcgcg cgggctcttc ggggagtgaa gttcgggtcg
      301 gagcgcgatc gtgcggcgtt cctggcagcg tctcctgccc gtgacgtcat cgctggcggg
      361 gatgacgctg tgccagtacc gatgcacgtg gctcgcgagc gagagcgcgg ctacaaccag
      421 gcggctctga tagcggagta tgcctgccgt gcgcttagcc gagagacatt tcgccgggcg
      481 ctggtgcagc acctagagcg tccgtcacag gtcggtctca acgcgcaaga gcggcgagac
      541 aatgttcgcc ggtcattctc cattgccggc gacgtcgttc catcaccgcg caagcggtat
      601 gtgctcgtcg acgacgtgcg aacgacgggg tcgactctgg cggcgtgtgt cgaggcgctg
      661 cggcccctgc gggctgcccg aattgacgtg gtgacgtttg ccgcggagtt gaggagtgag
      721 gtgatcgagg ccacggtcaa acctttccct gactcgtagg ctggtcagac gattgcccgc
      781 accacattgc tcgcttcgcg gcgcgcccac gcgtcggctg caaccacgtc gtctagcgac
      841 gcgacgtcgt gtgcggggtg gcgcgccatc acggtttcga gcagtcgtgg tatcccaggg
      901 aaggtgatgc tacctgaggc gaacgcccgc accgcgattt cgtccacagc actgtagacg
      961 gtagggtagg ttcgacccgc ttcgccggcc tctctggcaa ggcgcagcgc cgggaatcgg
     1021 tcgaggtccg gtgcctcgaa ggtcatggag ccaatcgaaa caagatccaa gggtctgctc
     1081 gctgtcgctg ccagccgttc gggccatgtg agggcgtact ggatcggcag acgcatgtcc
     1141 gggaagctga gctgagcgat actgtttcca tcgatgaact cgatcagcga gtgaatgatt
     1201 tgttcggggt gaaccacgac gtcgatccgg tcgaacggcg tgtcgaagag gtggtgcgcc
     1261 tcgataagct cgagcccctt gttgaccagt gtggcggagt cgaccgtgat cttggcgccc
     1321 atgctccagt tggggtgctt gagtgcctgc tcgtatgtca cggaggccag tgcgctgcgg
     1381 ggcgtcaacc gaaaggggcc gccggacgca gtgaggatga tccgctcgat agtgtcggcc
     1441 gtgcctgagg ccagagattg ccagatagcg ctgtgctcgc tatcgattgg gcggacggtc
     1501 acgccgtgct cgcgggccag cggcatgatg aggtcgccgg cacagacaat cgcttccttg
     1561 ttggcgaggg cgatctcctt gccctgcttg atcgcctcga ttgttgccgg gatcgcgtcg
     1621 tgccctgagg tggcgaacac cacgaggtcg acgtccgggt gggtagccgc tgcgaccagg
     1681 ccgtccggcg tcggctgcca ctcgatgccg tcgagcgctg cgtttcgttc cgcggagacg
     1741 acgagcgtgg gccggtgcgc tcgcacctgt tcccgcagca acttggtgtc acgaccggcg
     1801 gcgatggtga cgatcttgaa cgtgtcagga tgctgctcga tgacatcgat agtttggcgg
     1861 ccaatggagc cggtggaccc gagcagtgcg atgcgagtgg tcatggagaa tctccaactg
     1921 ggcgccagtc atggagtagt gcaccggcca ccgggtcact gcaggatgcg gacgatcacc
     1981 caggttacga gaaagatggg cagcagagca tcgacgcgat cgaagatccc cccgtggccg
     2041 gggatgagtg tgccactatc cttcacgcct ccgcatcgct tgataaacga ttcggagagg
     2101 tcgccgccga cgccgacaat ggacaggact acgccgatga cgatcgcctg cgcaacagag
     2161 acatccggta tgccgagcag gagggtcaag gtgacggtcc cgatgaccgc gccgaggacg
     2221 ccgcccacag cgccttccag cgttttcttg gggctgatat gcgggatgag cggcgtgcgt
     2281 cccatggtgt ggccgacgag aaatgcgaac gtgtcgctca gccaggccac gacgatggcg
     2341 atcgccgtcc acgccattcc aagtgcgtag gagtcatccg aaacggagaa gtaggtgccg
     2401 acgtcgttgg cccactcggc atcgaggata ccggcctcct ggcgaagagc aatcgcggca
     2461 aacaccgcga tgccgacgta ggcgcccacg gcggcagtgg tgatccacgc cgcgaagctc
     2521 tcgctgctga ccggtagttg cagaatcatc actgtcggca gcagtaccgc gatcgagatg
     2581 aagctggcga acgaccacgg ttcctgggac agcagcgaga tggacgcacc gaggaagatt
     2641 acgacgtacc ccggccactc aagccggtag ccgaggccga tcagcggtgt gatgcgcgag
     2701 gtttcatagt agacgccgag cccgatcgcg gccatcactg ccgcaaacac gggtccgccg
     2761 atcacgccgg cgattacgcc gaccgcgata acccccagtg aggaaacagc tctggtacgc
     2821 agcgcaccct cccgtcagcc agttatcgag gagttcgata tcgctccgaa tcgtcgctcc
     2881 cgctgcgcat agtcccgcac ggcggattcg agatcgccgg ggctgaagtc tggccagagg
     2941 gtagggctga agtgcagttc ggagtacgcg ccctgccagc agaggaaatt gcttagccgg
     3001 tattcgcccg acgtccgaat gatgaggtcc gggtcgggga gatcgtgcgt gtagaggtac
     3061 cgctcaatca tgtcctcatc gatgtcttct ggggccacgc ccgaggcgac gatgttctgg
     3121 actgcgcgag tcagctcctg gcgccccccg tagttgaacg caagggtcag caccagcttg
     3181 tcgttgtact tcgtgcgctc gatcgccccc tcgatcgctc cttggagatc gggcgagagg
     3241 gagcgcaggt cgccgatgtg ccgaagctgg gcaccctgcc ggtgcagctc ctccgtctcg
     3301 gtctcgatgg cacggctgag aatgttgaga aggccctcga cctcttcgcg cggacgacgc
     3361 cagttctcgg cggagaaggc ccacagcgtg agatagcgga tgcctacctg tcctgctgcg
     3421 tgcgtgatgc gccgaatgtt ttcggttcct gcctcatgtc cttcgaggcg cgccagcccg
     3481 cgttgccgcg cccatcgtcc gttgccgtcc atgatgatgg ccacatgccg cggcactcgc
     3541 gaggggtcga tcggcgtcga ggaactctcg tgagcggctg gggtgggtga cacggagcgc
     3601 ggcggatgtg ctgtcatccc tagacctcga ggacttcctg ttccttttcc ttgccgatct
     3661 cttcggcccg gtcgacgaac cgcttgctca gggcatcgat ctcatcgatc gcgcggcgct
     3721 catcgtcctg cgagatctcc ttgtccgtca gctgcttgcg cagatggctc acggcgtcac
     3781 gccggatatt gcgcacggcg accttgcttt cctcgacggt ctggtgaacg atcctgacga
     3841 gttgcttgcg tcgctcttcg gtcagggaag ggatcgagat gcggatcagt tgtccgtcgt
     3901 tgttcggtgt cagccccagg tcagagcttt ggatcgccct ctcgatcggg ccgatgctgc
     3961 ttcgatccca tggctggatg acgagcaggc ggggctcggg agccgagatg cccgcaagct
     4021 ggttcagcgg ggtctgtgcg ccgtagtagt cgacatggat tctgtcgagc agcgcagggg
     4081 tggca
//
LOCUS       DASYWL010000184        12685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_327434, whole genome shotgun sequence.
ACCESSION   DASYWL010000184 DASYWL010000000
VERSION     DASYWL010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 12685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12685
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327434"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VGR22_06510"
     CDS             complement(<1..210)
                     /locus_tag="VGR22_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128250.1"
                     /translation="MRTLIRASHIIAFQNGEHRHLQNGVIVVEDNEIVHVGKTFDGTA
                     DTEIDASDMVVTPGFINTHAHLYESP"
     gene            complement(366..1508)
                     /locus_tag="VGR22_06515"
     CDS             complement(366..1508)
                     /locus_tag="VGR22_06515"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV2128251.1"
                     /translation="MKIETFALERWMTTWELNVSHDITESGIAPMSIDDLLRIAEIDD
                     PAGTIRELMAAPQLYSEARGTEALRGIIAGMFETASADDVLVTTGAIEANFLVYNALL
                     EAGDHVVVTTPCYQQLISIPKAIGAEVSEWRVTPESGFTFDLDALDSLVTDRTKLIVI
                     NSPHNPTGAVLSQADAERIARIANRADAWVLSDEAYRWLTHPGGIELPGPFRDFYPKS
                     VSVSTMSKFFGLPGLRLGWLVGTAELAAECWSMRDYVSLSPAWLSDRFSQIAVANYRK
                     VQQRNHGILEKNLTKAREWFAENADIVSWSEPKAGLLAMMKVHGFENTDDLSEHLARD
                     VGVMLAPGSAFGLPGYLRIGIGQRPEIFAEALDRTEEAIRAYKART"
     gene            complement(1567..2880)
                     /locus_tag="VGR22_06520"
     CDS             complement(1567..2880)
                     /locus_tag="VGR22_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128252.1"
                     /translation="MSEAVASSAIREAPGLVRYPIARLFSTGEQLLLHPILVLPILVW
                     LLGGTPGQIVWYAIISGIGAGLAAASGALVARLPATARHTIVGLLALQGIGVLVAIAV
                     SLGTSSFGNTTLLTLGALAYLLITLPSAMLTRISEQNHEYRRAASATVGGVLPAIVGT
                     TLGAIIIWRLFFIGGLGPDDMIGRLLLAGALFAAAGSWLATWPTLLARQLPHPARPMP
                     TVRWPALRTNRPLQRYSLFQAVRGIAHFADPFLFVGVLTIIEPGIAWIGGAALAWALG
                     EAVARLLAVRAYGAINVRGVFTVSGTLHAVAYIIIAFAADVLGSSLIAERAAGDQWRN
                     WAVVLAALALGASHRFARTGHHAYIRSISSPGTRDLSLTVTGIVVIIFAFAPLVAVRL
                     LESQDLATLLQIGAGASIIGLLATGLVVPTYAAPRRPRGAWGMRR"
     gene            complement(2969..3793)
                     /locus_tag="VGR22_06525"
     CDS             complement(2969..3793)
                     /locus_tag="VGR22_06525"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEV2128253.1"
                     /translation="MRYIGGDVREEGCIFCNRLAADNDVRSLVLHRSGHSFVIMNLYP
                     YNTGHVMAVPNAHKANLDALDEPTLIDMATLLPKVVTSLRRVLNCDGFNIGLNLGADA
                     GAGVAQHLHQHVVPRWEGDANFMPILAGTMVIPEVIPATYAKIRAELDRVLDGTSTFD
                     IVVISDEERPAAWLRDGTIPTVSPDGDTPVWRAARAAIATEGAEIVGWSGASSTAGSE
                     RRPGLVLRTQGMPTSGDWHAVPLRAPGDDVDAETAATLARSRENLFPWIDAAEGHE"
     gene            complement(3943..4863)
                     /gene="hisI"
                     /locus_tag="VGR22_06530"
     CDS             complement(3943..4863)
                     /gene="hisI"
                     /locus_tag="VGR22_06530"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF000768.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEV2128254.1"
                     /translation="MTPDTTLPLRFGQDGITPAVIVDATSGAVLMVGFMNEEALQRTR
                     DTGCVHFWSRSRQKLWKKGESSGHLQHVREVRVNCDQNSLLIEVDQDGAVCHDGYDTC
                     YYRRLEPDNSLTIVRDRQFDPRDVYPKDGDPAGLATRTEEWWQAYEWLRDSNVEADSG
                     TSKRLRADEDAHTPRLSDELRELAGVLDGFHRHASLDEDIRLESGQVLYWAVCTAVWQ
                     RLAWSDVRPDRALGITGGEQPSSATLSRLLTARADELVTRAMPPDAPLLHDTFGLVGT
                     VLQAHDVDPLDVIKADLADLSAKPYIPRDQ"
     gene            complement(4853..5653)
                     /gene="hisF"
                     /locus_tag="VGR22_06535"
     CDS             complement(4853..5653)
                     /gene="hisF"
                     /locus_tag="VGR22_06535"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEV2128255.1"
                     /translation="MPDSDNNGLTTRVIPCLDVTAGRVVKGVQFVDLRDAGDPVEMAA
                     IYNREGADELIFLDITATSDSRDTMIDVVRRTADQVFIPLTVGGGIRTVQDMRSMLGA
                     GADKVSVNSAAIRDPELITRGAEIFGTQCIVVAIDARWTPQHDRWEVYTHGGRKPTGI
                     PVTDWAAEAERRGAGEILLTSMDRDGTGAGYDLDLLEKVSKVTRIPVIASGGAGNAHH
                     MVEALQPGRADAVLAATIFHFGELRVSEVKRELAEAGLRVREMPIDDA"
     gene            complement(5646..6365)
                     /gene="hisA"
                     /locus_tag="VGR22_06540"
     CDS             complement(5646..6365)
                     /gene="hisA"
                     /locus_tag="VGR22_06540"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEV2128256.1"
                     /translation="MIVYPAIDIRGGKAVRLVKGDFNRETGFDADPADAARRWESAGA
                     EWIHVVDLDGARTGAGENRDAIAGIKDTVSCKLQVGGGVRSMDDIDTLLGTSIDRVIL
                     GSIAVTNPETVVDAAHKYGGRIAVALDARDGKLATSGWETQTEADAFETARQMSDHGV
                     RHIIFTDIRRDGTLAGPNIGALTHMVESTSAGVIASGGIGTADDVLGLGATGVEGVII
                     GRALYDGRIDLADLLRRLADA"
     gene            complement(6362..6973)
                     /gene="hisH"
                     /locus_tag="VGR22_06545"
     CDS             complement(6362..6973)
                     /gene="hisH"
                     /locus_tag="VGR22_06545"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEV2128257.1"
                     /translation="MTSIVDYGAGNLRSIERALVHFGDEPMITGDPDQIRKADAIIFP
                     GVGNARAAMEHLRATGIADAMTEATRAGTPLIGVCLGMQLLFGDQEEGPTTGIGLLDG
                     VGVRLPGSLKVPHMGWNTVRFTANGPLADLPDAPFYFVHSYVVQPADPKDVAGETDYG
                     ITFPSVVVHENVWGMQFHPEKSGDIGLALLQRWLVFARSQVTA"
     gene            complement(6970..7560)
                     /gene="hisB"
                     /locus_tag="VGR22_06550"
     CDS             complement(6970..7560)
                     /gene="hisB"
                     /locus_tag="VGR22_06550"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF002111.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEV2128258.1"
                     /translation="MSGRTGSISRTTSETSITVLIDLDDASEGSLSTGVGFFDHMLHA
                     LARHGRFGLTVDASGDLHIDAHHTVEDVGIALGQAFRKALGDRVDIERYGHAYAPLDE
                     ALARVVIDCSGRPFLEFNATFPEASIGEQFDASLIEEFWRGFAVHAGLTIHIDLLRAR
                     NSHHAAEAIFKAGAISLRTAVRQTGSGVPSTKGVLQ"
     gene            complement(7557..8660)
                     /gene="hisC"
                     /locus_tag="VGR22_06555"
     CDS             complement(7557..8660)
                     /gene="hisC"
                     /locus_tag="VGR22_06555"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEV2128259.1"
                     /translation="MAFSPERVVRHIAPEGAYHRSEPETGGTPSVRLDWNESPFGLSP
                     KAQAAYDTFRTGNIYPAIDQAPLREAIGRYLDVPAGRIIVGAGLDDVINTIAMTVIDP
                     GTEVIISEPTFGVYRSLFELHGATVTDVPLGPAPDFALPAEAIIEQVSERTRLVIVCN
                     PNNPTGTLFPRREIVRIIESVDCLVSIDEAYAEFSGVSHTDLARQHGNAITLRTMSKF
                     AGLAGFRVGYGVMPEAVMPWVRRAAPAFYNVSAPAAAVAIASLRDLDHLRANAERIIA
                     ERDRLSAALGAMDGVTVYPSATNFLLVAMPMPDASPMVSALAREGLLVRGYSDPNLRQ
                     CIRISIGLPEHHDRLIDVVERELAAQREGVTQP"
     gene            complement(8702..9595)
                     /gene="hisG"
                     /locus_tag="VGR22_06560"
     CDS             complement(8702..9595)
                     /gene="hisG"
                     /locus_tag="VGR22_06560"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEV2128260.1"
                     /translation="MTSARSLFNDGSTLKLAVQRKGRLTEHSMSLLHQIGLNFESYGQ
                     RLFSPVRNFPLSLLYARDDDIPGYVTLNTVDLGIVGKNLVHEYEADVEIIHPLGFGYC
                     ELVIAVPNESEVQTPEDLRNSRIATSYPSSTRRYFESIGGNPEIITVSGSVEITPALD
                     IADAIVDLAATGSSLQLNDLRPIHSILESEAILIANGEALGDPGRRENIDRLLMRIKA
                     VEAAEQYKYVMMNAHADSLPAIRKVIPGLKAPTIVPLADEGWYAVHTAIREEDFWKKI
                     EELRAAGASEILVSALDKLLL"
     gene            complement(9776..10201)
                     /locus_tag="VGR22_06565"
     CDS             complement(9776..10201)
                     /locus_tag="VGR22_06565"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEV2128261.1"
                     /translation="MERPKLQPHLINLPDAPLARLLVGIGRAFRRHCPYCGGGHIFDG
                     WFTLKKQCPTCGVTYAYETGYFLGSYVVNIVVTEFVAVAAVVALLIWSDLSVLQLQIA
                     GVVLAVGLPILFYPFALLLWVALDVTFHPPNPATGKRDL"
     gene            10290..11063
                     /locus_tag="VGR22_06570"
     CDS             10290..11063
                     /locus_tag="VGR22_06570"
                     /inference="COORDINATES: protein motif:HMM:NF024931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HEV2128262.1"
                     /translation="MSRLATAPNGVRAWGLGAGRVLAKVFSCAVIGVLVEVEVDVGAG
                     QPSMVLVGLPDAAVQESRERVRAAIRNSGGRFPHGRVTVNLAPADLKKAGPTYDMPIA
                     IGILLASQAVLAPLDDALLVGEMSLVSVAAERGMKRAFVPAVDAQEAALVAGITVYPV
                     RNLSELVQHLNGFATIEPVETVRSLANLNGHQPVVDFADIRGQEHVKRGMEVAAAISL
                     TITLTKPSRASAPSAAGGRLERSLSTFVVTEGLGPLKRT"
     gene            complement(12185..>12685)
                     /locus_tag="VGR22_06575"
     CDS             complement(12185..>12685)
                     /locus_tag="VGR22_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128263.1"
                     /translation="AALAVALLIFVVADFAVSALSSYLIARREDAAVDWPGVWLLLRG
                     ITGAWLILAVWAALGVLLAVLSRGTALAIGIGILYGLVIEGLISTLFDQVRLLRPLAQ
                     ALLRANAYSLVEPLGVSVTTAAGAGPGMFTGPYVGGVQASLVLTAYLVSFVLLAAILL
                     RQRDVT"
BASE COUNT         2247 a         3812 c         4383 g         2243 t
ORIGIN      
        1 cggcgattcg tagaggtgcg cgtgggtgtt gatgaagcca ggcgtcacga ccatgtcgga
       61 ggcgtcgatc tccgtgtcgg cggtgccgtc gaacgtcttg ccgacgtgga cgatctcgtt
      121 gtcctcgacg acgatgacgc cgttctggag gtggcgatgc tcgccgttct ggaaggcaat
      181 gatgtggctt gcgcgaatca gcgtccgcat ggatcgtgtg ctccttgtgg tggctgtcgg
      241 ataccgagag cgggtttccg cagcggcatg gcaacgcaga actctcgtgc tcctgtcgtg
      301 aactgggagg ggcgaattag ctgccagctt ccctcgcttg gcgagcagcg gtgcggcttc
      361 ctcttctacg ttcgggcctt gtacgctcgg attgcctcct cggttcggtc gagcgcctcg
      421 gcgaaaatct cggggcgctg gccgatgccg atgcgcaggt agcctggcag gccgaatgcc
      481 gaccctggcg ccagcatcac cccgacgtcg cgggcaagat gctcggagag gtcatcggtg
      541 ttctcgaacc catgaacctt catcatcgct aacaggcctg ccttcggttc agaccaggag
      601 acgatgtccg cattctcggc gaaccactcc cgcgccttcg tcaggttctt ttcgagaata
      661 ccgtgattgc gctgctggac cttccggtag ttggcgaccg cgatctggga gaagcgatca
      721 ctcaaccacg ccgggctcag cgagacgtag tcgcgcatgg accagcactc ggccgcgagc
      781 tccgctgtgc cgaccagcca tcccagtcgc agtcccggca ggccgaagaa cttcgacata
      841 gtgctgacac taacgctctt tgggtagaaa tcgcggaagg ggccgggcag ttcgatgccg
      901 ccggggtggg tgagccatcg gtaggcctcg tcagagagca cccatgcatc ggcccgattg
      961 gcgatcctgg cgatgcgctc agcgtccgcc tgcgacagca ccgcgccagt ggggttgtgc
     1021 ggcgagttga tcacgatcag cttcgtgcgg tcggtgacga ggctgtcgag ggcatccagg
     1081 tcgaacgtga agcccgactc cggcgtcact cgccattcgg agacttccgc gccgatcgcc
     1141 ttgggaatgc tgatgagttg ctgatagcag ggtgtggtaa cgacgacatg gtcgccggcc
     1201 tccagcagcg cattgtagac gaggaagttt gcctcgatgg cgccggtggt caccaggacg
     1261 tcgtcggccg aggcggtctc gaacatcccc gcgatgatgc ctctcagcgc ttcggtgccg
     1321 cgcgcctcgc tgtagagctg cggcgcggcc atgagttcgc ggatcgttcc ggccgggtca
     1381 tcgatttctg cgatacgcag caggtcgtcg atggacatcg gcgcgatgcc gctctccgtg
     1441 atgtcatggg agacgttgag ctcccacgtt gtcatccatc gctcgagggc gaaggtctcg
     1501 attttcatgt ggtatcggtg ctccagagat catcacccgc actgtgcagg cgccagttcg
     1561 actggctcag cgtcgcatgc cccacgcgcc acgcggccgc cgaggggctg cgtacgtcgg
     1621 gaccacgagt cctgtggcca acaggccgat gatgctggca ccggcaccga tctgcaatag
     1681 ggtggcgaga tcctgggatt ccagcaggcg caccgcgacg agtggggcga aggcgaatat
     1741 gatcaccacg atgccggtga cggtaagcga gaggtcgcgc gttcccggcg agctgatcga
     1801 gcggatgtag gcgtggtggc cggtgcgcgc gaagcgatgg ctggcgccga gtgcgagtgc
     1861 ggcgaggacc accgcccagt tgcgccattg gtccccggcg gcgcgctcgg cgatcagcga
     1921 gctgccgagc acgtctgcgg cgaaggcgat gatgatgtag gcgactgcgt ggagagttcc
     1981 gctcacggtg aagacgcccc ggacgttgat cgctccatat gccctgaccg cgaggagtcg
     2041 ggcgaccgct tcacctaacg cccaggcgag cgccgcgccg cctatccagg cgatacccgg
     2101 ctcgatgatg gtgaggaccc cgacgaagag aaaggggtcg gcgaagtgag cgatgccacg
     2161 caccgcctgg aagagcgagt agcgctgcag ggggcggttc gtgcggagcg cgggccaccg
     2221 gaccgtcggc atcggtcggg ccggatgggg cagttgtcgt gcaagcagtg tgggccaagt
     2281 cgcgagccac gatccggcgg cggcgaagag cgcgccggcg agcagcagcc gtccgatcat
     2341 gtcgtccgga ccgagtccgc cgatgaagaa gaggcgccag atgatgatcg cgccgagggt
     2401 ggtgccgacg atggcgggca gcacaccgcc gaccgtcgcc gacgcagccc gccggtactc
     2461 gtggttctgc tcggagatgc gggtgagcat ggcggacggg agcgttatga gcaggtaggc
     2521 gagcgcgccc agcgtgagca gtgtggtgtt gccgaagctg gacgtgccga ggctgaccgc
     2581 gatggcaacc agaacaccga ttccctgcag ggcaaggagt ccgacgatgg tgtgacgagc
     2641 ggtggcaggg aggcgggcga ccagtgcgcc agaggctgcg gccaggccgg ctccgatgcc
     2701 actgataatc gcgtaccaga cgatctgtcc cggcgtgccg ccgagcagcc agacgaggat
     2761 gggcagaacc aggatgggat gcaggagcag ctgttcgccc gtggagaaga ggcgggcgat
     2821 ggggtagcgg acgagaccag gtgcctcacg gattgcggag ctggcgaccg cttcggacat
     2881 gtggaactcc atcggtcagg tgatcgggct catcggctgg tccttccaca cggctgccgg
     2941 tggcgacccc gctcgagagt gtagccgatc actcatgacc ctcggctgca tcgatccacg
     3001 ggaagaggtt ctcgcgagat cgcgcgaggg ttgctgccgt ctcggcatcc acatcatccc
     3061 cgggcgcacg aagcgggacg gcatgccagt cgccacttgt cggcatgcct tgggtacgga
     3121 ggacgaggcc cgggcggcgc tcgctgccgg cagtggacga agcgccgctc cagccgacga
     3181 tctccgcccc ctcggtggcg attgcagcgc gagccgcgcg ccagacgggc gtatcgccgt
     3241 ccggcgagac cgtcgggatt gtgccgtccc gaagccaggc agcgggacgc tcctcgtctg
     3301 agatgacgac gatgtcgaag gtggaggtgc catcgagcac ccgatccagc tcggcgcgaa
     3361 tcttggcgta ggtggccggg atgacctcgg ggatcaccat cgtgccggcg aggatcggca
     3421 tgaagttggc gtcgccctcc cagcggggaa cgacgtgctg gtggagatgc tgagcgactc
     3481 cggcaccggc gtcggcgccg aggttgaggc cgatgttgaa gccatcgcag ttgagcacgc
     3541 gccgcaggga cgtgaccacc ttcggcagca gggttgccat gtcgatcagg gtcggttcgt
     3601 cgagggcatc gagattcgct ttgtgggcgt tcggcaccgc catgacatgc ccagtgttgt
     3661 acgggtagag gttcatgatc acgaacgagt ggccagaccg gtggaggaca agcgatcgca
     3721 catcattgtc cgctgcaagc cgattgcaga agatgcagcc ttcctcacgg acgtcgccgc
     3781 cgatatagcg catccgccag ggggtccaaa gccgttcggg atgagatgat cggtggggag
     3841 ccgttcggcc cggttcgcga ttgttttctc caggggtccg ctgctctcga gccatggtct
     3901 gcttctccac ccctgagcca gggtcgcacg cggcgcggct ggctactggt ctcggggaat
     3961 gtagggtttg gcactgaggt cggcgaggtc ggccttgatg acgtccagcg ggtcaacgtc
     4021 gtgggcctga aggaccgtgc cgacgaggcc gaaggtgtcg tgcaggaggg gcgcgtcggg
     4081 tggcatggcg cgcgtgacca actcgtcggc gcgggccgtc aggagccgcg agagtgttgc
     4141 cgacgacggc tgctctccgc cggtgatgcc gagcgcgcgg tcgggccgga catcgctcca
     4201 tgcgagccgc tgccagaccg ccgtgcaaac tgcccagtaa agcacctgcc cggactccag
     4261 ccgaatgtcc tcgtcgagcg atgcgtggcg gtggaagccg tccagcaccc cggccagctc
     4321 gcgcagctca tcggagagac gcggcgtgtg ggcgtcctcg tctgcacgca gccgcttcga
     4381 ggttcccgag tcggcttcca cgttgctgtc gcgcagccat tcgtaggcct gccaccattc
     4441 ctccgtacgg gtggcgagcc ctgcaggatc tccgtctttg gggtagacgt cgcgcgggtc
     4501 gaactgccgg tcgcggacga ttgtcaggct gttgtccggc tccagacggc ggtagtagca
     4561 ggtgtcgtag ccatcgtggc agacagcacc atcctggtcg acttcgatca gcaggctgtt
     4621 ctggtcgcag ttgacgcgaa cttcccgcac gtgctgcagg tggccggagg actcgccctt
     4681 cttccacagc ttctgccggc tacggctcca gaaatggacg cagccggtgt cgcgagtgcg
     4741 ctggagcgcc tcctcgttca tgaacccgac catcaggacg gcgccgctcg ttgcatcgac
     4801 gatcacggcg ggggtgatgc cgtcctgccc gaaccgaaga ggcagggtgg tgtcaggcgt
     4861 catcgatcgg catctcccga accctcaggc ccgcctccgc aagctcacgc ttgacctccg
     4921 acacccgcag ctcgccgaag tggaagatcg ttgcggcaag gacggcatcg gcccggcccg
     4981 gctggagcgc ctcgaccatg tggtgggcgt tgcccgcgcc gcctgaggcg atcaccggga
     5041 tgcgggtcac cttgctgacc ttctccagca gatcaaggtc ataacccgcg ccggttccgt
     5101 cgcgatccat cgaggtgagc aggatctctc cggcaccgcg ccgctcggcc tcggcggccc
     5161 agtcggtcac cgggatgccg gtcggcttgc gaccgccgtg ggtgtacacc tcccatcggt
     5221 catgttgagg ggtccagcgg gcatcgatcg cgacgacgat gcactgggtg ccgaagatct
     5281 ccgcgccgcg ggtgatcaat tcggggtcgc ggatggccgc agagttcacc gaaaccttgt
     5341 ccgcacccgc gccgagcatg ctgcgcatgt cctgcacggt gcggatgccg ccaccgacgg
     5401 tgagcgggat gaatacctga tcggctgtac gacgcacgac gtcgatcatg gtgtcgcgac
     5461 tgtcggaggt ggcggtgatg tcgaggaaga tcaactcgtc ggcgccctcg cggttgtaga
     5521 tggcagccat ctcgaccggg tccccggcgt cgcgcaggtc aacgaactgg acgcccttga
     5581 cgacgcggcc ggcggtgacg tcgaggcagg ggatgacccg ggtggtgagg ccattgttgt
     5641 cggaatcagg catcggcgag cctcctgagg agatcggcaa gatcgatgcg accatcgtag
     5701 agcgcccggc caatgatgac gccctcgaca ccggtagccc cgagaccgag cacgtcgtcg
     5761 gccgtgccga tgccgccgga ggcgatcaca cccgcgcttg tgctttcgac catgtgtgtc
     5821 agcgccccga tgttcgggcc ggcgagggtg ccgtcccggc gaatgtcggt gaagatgatg
     5881 tgccttactc cgtgatcgct catctggcgg gcggtctcga aggcgtcggc ctccgtctgg
     5941 gtctcccatc cggatgtggc cagcttgcca tcgcgagcat ccagcgcgac ggcgatgcga
     6001 ccaccgtact tgtgggctgc gtcaacaaca gtctcgggat tggtgacagc gatcgagccg
     6061 agaatgacgc gatcgatgct ggtgccgagc agcgtgtcaa tgtcgtccat cgagcgaaca
     6121 ccaccgccga cctgcagctt gcaggagaca gtgtccttga tgccggctat ggcgtcgcgg
     6181 ttctcacccg caccggtgcg agctccatcg agatcaacga cgtggatcca ctcggcgccg
     6241 gccgattccc agcgccgggc ggcatcggcg gggtcggcgt cgaatccggt ctcacggttg
     6301 aaatcgcctt tgacgaggcg gaccgccttg ccgccgcgga tgtcgatagc ggggtagacg
     6361 atcatgcggt gacctggctc cttgcgaaca cgagccagcg ctgcaggagc gcgagcccga
     6421 tatcaccgct cttttcgggg tggaactgca taccccagac gttctcgtgt acaacgacac
     6481 tggggaaggt gatgccgtag tcggtctcgc cggcaacgtc ctttggatcg gcaggctgca
     6541 ctacgtagga gtgcacgaag tagaagggcg catccggcag gtcggcaagc gggccgttgg
     6601 cggtgaagcg cacggtgttc cagcccatgt gcggcacctt gaggcttccg gggagacgta
     6661 cgccaacgcc atccagcaag ccgatcccgg tcgtcgggcc ttcctcctga tcgccaaaaa
     6721 gcaactgcat gccgaggcag acgccgatca atggagtgcc cgcgcgtgtt gcctccgtca
     6781 tggcgtcggc gataccggtc gctcgcagat gttccattgc cgcgcgggca ttgccgacgc
     6841 cggggaagat gatcgcgtca gccttcctga tctggtcggg atcgcccgtg atcatcggct
     6901 cgtcgccgaa gtggacgagc gcgcgctcga tcgagcgcag gtttccggca ccgtagtcga
     6961 cgatggatgt cattggagca cccccttggt ggacggcacg ccgctgcccg tttgacggac
     7021 ggcggttcga agagagatgg ctccggcctt gaagattgcc tcggcggcgt ggtggctgtt
     7081 gcgggcacga agcaggtcga tgtggatggt caggcctgcg tgcaccgcga accctctcca
     7141 gaattcctcg atgaggctgg cgtcgaactg ctcgccaatg ctcgcctccg ggaaggtggc
     7201 gttgaactcg agaaatggcc ggcccgagca atcgatcaca acccgcgcga gtgcctcgtc
     7261 gagcggggcg taggcgtggc cgtagcgctc aatgtcaacg cggtcgccga gcgccttgcg
     7321 gaacgcctgg ccaagcgcga tgccgacatc ctcaacagtg tggtgggcgt cgatgtggag
     7381 gtccccggag gcgtcgaccg tcaggccgaa gcgtccgtgg cgtgccagcg catgcagcat
     7441 atggtcgaaa aagccaacgc cggtgctgag cgaaccctcg gaggcgtcgt cgaggtcgat
     7501 gagcacggtg atgctggtct cgctggtggt tctgcttatg gaaccggttc tgccgctcat
     7561 ggctgcgtga ctccctctcg ctgggcggca agctcgcgtt caacgacgtc gatcagccgg
     7621 tcgtgatgct ccggcagccc gatggagatc cgtatgcact gcctgagatt cggatcgctg
     7681 tagccgcgca cgagaaggcc ttccctggcg agtgcgctca ccattgggga agcatcgggc
     7741 atgggcatcg cgacgagcag gaagttggtc gcagacggat agaccgtcac gccgtccatc
     7801 gcaccaagcg cagctgaaag tcggtctcgc tcggcgatga ttcgctcggc attggctcgg
     7861 aggtggtcga ggtcacggag cgaggcgatc gccacggccg ctgccggagc cgagacgttg
     7921 tagaaggccg gggccgctcg gcggacccag ggcatcaccg cctccggcat gacgccgtag
     7981 ccaacgcgga acccggccag tccggcaaac ttgctcatcg ttcggagtgt gatggcgttt
     8041 ccgtgctgcc gggcgagatc ggtgtggcta acgccggaga actcggcata ggcctcatcg
     8101 atggagacca ggcagtcgac cgattcgatg atccgcacaa tctccctacg agggaagagg
     8161 gtgccagttg ggttgttcgg gttgcagacg atgaccagcc gggttcgctc ggacacctgc
     8221 tcgatgatgg cctccgccgg cagggcgaag tctggcgccg gacccagcgg cacgtcggtg
     8281 accgtcgcgc cgtgcagctc gaagaggctg cggtagaccc cgaaggtcgg ctcgctgatg
     8341 atgacctcgg tgccggggtc gatgactgtc atcgcgatgg tgttgatgac gtcgtcgagg
     8401 cctgcgccga cgatgatgcg accggcgggc acgtcgagat agcgcccgat tgcctcgcgc
     8461 aagggtgcct ggtcgatggc tggatagatg ttgccggtcc gaaaggtgtc gtaggccgct
     8521 tgggccttcg gcgaaagtcc gaacggcgac tcattccaat caaggcgcac ggagggcgtg
     8581 ccgccggtct ccggctcgga acgatggtaa gcgccctccg gcgcgatgtg gcggacgacc
     8641 cgttcgggtg agaacgccat ggcggcctcc gtgagacgtg tggtgcgatc ggtcggcgag
     8701 atcagagcag cagtttgtcg agtgcgctga cgaggatctc gctggcgcca gcggcacgga
     8761 gttcctcgat cttcttccag aagtcttcct cacggatggc agtgtggaca gcgtaccagc
     8821 cttcatcggc gagcggcacg atggtcggcg ccttgagacc gggaatgacc ttgcgaatgg
     8881 cgggcaggga gtcggcgtgt gcgttcatca tgacgtactt gtactgctcg gctgcctcga
     8941 ccgccttgat tcgcatcagg aggcggtcga tgttctcgcg tcgacccgga tcgcccagcg
     9001 cctccccgtt ggcgatcagt atggcctccg actcaaggat cgagtggatc gggcgcagat
     9061 cgttgagttg cagcgaggag cccgtggcgg cgagatcaac aatggcgtcg gcgatgtcga
     9121 gcgctggagt aatctccacg gagccgctca ccgtgatgat ctccggattt ccgccgatcg
     9181 attcgaagta gcggcgtgtc gaggagggat agctggttgc gatacggctg ttgcgcagat
     9241 cttccggtgt ttggacctcg gactcgttcg gcacggcgat caccaactca cagtagccga
     9301 agccgagcgg gtggatgatc tcgacgtccg cttcgtactc gtgcacgagg ttcttgccga
     9361 ctatgccgag gtcgaccgtg ttgagggtca cgtaaccggg gatatcatcg tcgcgggcat
     9421 agagcagcga gagcgggaag ttgcgcacag gcgagaagag acgctggccg tagctctcga
     9481 agttcaggcc gatctgatgc aacagcgaca tcgaatgctc ggtcagacgg cccttgcgct
     9541 ggacagcgag cttgagcgtg gagccgtcgt tgaacaatga tcgcgctgag gtcatgaccg
     9601 agggctttcc agtgagaggt gatccaccag gcaaggggac gtcgactccg ccggattggc
     9661 gggaatcttc ggttcttgta ccacgctcgt gattccatgc acaagcgccg gttcagcggc
     9721 tccgtgccgg gcgcacgccc gctcccgtgt cgcatagcac gcgtccgagc gtgggttaga
     9781 ggtcgcgctt gcctgtggcc ggattgggcg ggtggaaggt gacatcgagc gccacccaga
     9841 gcagcagcgc gaatgggtag aagaggatcg gcaggccgac ggcaagcacc acgccggcga
     9901 tctgcagctg cagcacactg agatcagacc agatgagcag cgcgacgacg gctgcgacgg
     9961 cgacgaactc ggtgacgacg atattgacga catacgagcc gaggaagtag ccggtctcgt
    10021 aggcataggt gacgccgcag gtcgggcact gcttcttgag cgtgaaccat ccgtcgaaga
    10081 tatggccacc gccgcagtag gggcagtgcc gacggaacgc ccggccgatg ccgacgagca
    10141 gccgtgcgag cggtgcatcc ggcagattga tgaggtgggg ttggagcttg ggacgttcca
    10201 tgatgatgcc tcgatctcga cgggggatgt ggccaagccg ggcattacag gctttctatt
    10261 gaatggtaca ttgctcgcag taaaccaaca tgtcaagact ggcaacggca cctaatgggg
    10321 tgcgggcgtg gggcttgggg gcgggaagag tgctggcgaa ggtcttcagc tgcgcagtga
    10381 tcggcgtgct cgtggaggtg gaggtcgatg tcggggccgg gcagcccagc atggtgctcg
    10441 tgggtctgcc ggacgctgcc gtgcaggaga gtcgagagcg tgtacgggca gcgattcgca
    10501 attccggcgg cagatttccg cacgggcggg tgacggtcaa tttggcgcca gccgatctca
    10561 aaaaggctgg cccaacctac gacatgccga tcgccatcgg catcctgctg gcatcccagg
    10621 cggtactggc gccactggat gacgccctgc tggtgggcga gatgtcgctg gtatccgtgg
    10681 cggcggagcg gggcatgaag cgcgcgttcg tgccggcggt tgacgcgcag gaagccgcgc
    10741 tcgtggcagg catcacggtc tatccggtgc gcaatctctc cgaactggtg cagcacctca
    10801 atgggttcgc aaccatcgag ccggtggaga cggtgcgatc gctcgcaaat ctcaacggcc
    10861 accagccggt agtcgacttc gctgacatcc ggggacagga gcatgtgaag cgggggatgg
    10921 aggtcgccgc ggctatcagt ttgacgatca cgttgacgaa accaagccga gcatctgccc
    10981 cgagtgcggc aggcggccgg ctcgaacgat cgttatcgac gttcgtagtg acggaaggcc
    11041 ttggtcccct gaaaaggacg tgatgcctag tgatgcctag gcttcagtgc accgctccca
    11101 ccaacgccgg accgtctcgt cacgcagaag attcgtggcc aaccgaaaac ctgcgtctta
    11161 cccactcggc cactgtgaaa aagcagcctg ctgcgaaccg gcaccgtact gatcacggca
    11221 aacgcaagcg ccgccatcac gcctcgtttt gagtagctgc gtcagaacct tggcccgcac
    11281 aactacgacc actctatcct tgggtcagtc ccggtcgaaa ccatcgcagt tgaaacccac
    11341 gccatgcgcg gcatggtctc gcacctcggg catgattcga taacccttga ggtggccgta
    11401 cttacggtca gcgatgggaa catcctcttc gtcacccttg gtaccgacta ctctctccac
    11461 tagacgacat gatcgagttg accaagcgcg ggttggagat ggagctgatc gattgacctc
    11521 ctgttttcga tactgctaat gagccaggtg ggctgtgaga atcggcatcc tgtattgacc
    11581 acatgcggca aagtttcacc tgagactggg attactgccc aagcgtcggg gtcttcggcg
    11641 tcaaccccta cctgtcagat gctacgccat ctgcttaccc ggttgcgacg ccgggccagc
    11701 tatagacaga acaagccccg gctccatgga acgctgcgcc gtgctggtgc cgggagggct
    11761 tacgatgacg tgaagtattt agcgttgtca tatgctatat ttccggttgc tagctaacaa
    11821 gttaattata atattaggaa aactgtcgac atgctggata tcgcgctaga acacttcaag
    11881 cgagcatcca agactgacgt cttgagaatg agtggtgatg agaatccgca tcggccttca
    11941 cgggagcttc gggcatgtgt tgccttgaag tgatggtatg cacctgctaa cgccgatgcg
    12001 ggaccgcgcc acgcacgagc gatggaattg cttccactag ggatgactgg tggaagaacg
    12061 ctctatctca gcaccgctgc gctagggaca acgaacggtc ggccccgtgg cggccgggtc
    12121 tgctgccgca gtggggaggg gccaagccct ctcaacgggt aacgtgcccc ctcccctcgg
    12181 ccgatcaggt cacatcccgc tggcggagca ggatcgcggc gagcaggacg aaactgacga
    12241 ggtaggccgt gagcacgagc gatgcctgga cgcccccgac gtagggaccg gtgaacatgc
    12301 cgggacccgc ccccgccgcc gtggtgaccg agacgccgag gggttcgact agcgagtagg
    12361 cgttggcgcg cagcagggcc tgtgcgagcg gacgcaacag gcgcacctgg tcgaacagtg
    12421 tgctgatcag cccctcgatt accaggccgt agaggatgcc gatgccgatg gcgagggctg
    12481 tcccccggga gaggacggcg aggaggacgc cgagcgcggc ccagacggcc aggatcagcc
    12541 aggcccccgt gatcccacgc agcagcagcc acacccctgg ccagtccacg gcggcgtcct
    12601 cgcgacgcgc gatcagatag ctgctgagcg cgctgacggc gaagtcggcg acgacgaaga
    12661 tgaggagggc caccgccaac gccgc
//
LOCUS       DASYWL010000185         2443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_364839, whole genome shotgun sequence.
ACCESSION   DASYWL010000185 DASYWL010000000
VERSION     DASYWL010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364839"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(337..1221)
                     /gene="proC"
                     /locus_tag="VGR22_06580"
     CDS             complement(337..1221)
                     /gene="proC"
                     /locus_tag="VGR22_06580"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEV2128264.1"
                     /translation="MTKQDKGDKKKGRKAEAALASTSIGFVGAGVMAESIIAGLLAKG
                     MLPPAQIIASHPRADRRERLADRYGVQVTASNREAAADADLVLLTIKPQVLRKVMQEL
                     HGQLSPRQVVISIIAGPALSTLRRGLGHDAVVRVMPNTPAQVSEGMSVWAATEAVSGE
                     QRDAVRSVLAALGEEIEVETEKYVDMATALSGTGPTYVFLMMEALVDAGVHMGFPRHL
                     AEEIVLQTVSGSVSFARSSGRHLAELRNMVTSPGGTSAEAIYQMEKGGLRTVLSKSVY
                     AAYQRTQTLAAEQESQDG"
     gene            complement(1293..2177)
                     /locus_tag="VGR22_06585"
     CDS             complement(1293..2177)
                     /locus_tag="VGR22_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128265.1"
                     /translation="MVFLTFPMVALTAQSDGGETDGFLFRATGDVTIGLDETISSVIV
                     ADRSAVVEGEVTDFLMVIGGTATVDGTVGGDIVMIDADLNLGQAATVENVTLFDSSLD
                     RAEGAIIAGDLSEEGDLTAFGWGAFVFSVMMWIGFMLLLIVAAVLFAWLGRRPLTSAG
                     EVLEQRVGASLLTALVLLIGLPLLAVLAFATVIGIPIGLAVFVVVIPALWLFGYLVAS
                     AKIGTLLLRPTNVRNLMLAAALGVLLLQVLTLIPALGPLVAFLATTYGAGGLVYWMVG
                     QPGRPQATAAQPRSRPVA"
BASE COUNT          469 a          841 c          755 g          378 t
ORIGIN      
        1 tcatggcgtt gatgtaagcg gacgggctgg ctggcgaggt tgtcaacctg ggcaacccgg
       61 aggagcgagc gcaccgtgcg ccagttggcc gagcgcatcg tcgacctcac cgggtgcgag
      121 ggcggacttc gattcgagcc cctccctcag gacgacccgc gacagcgtcg gcccgatatc
      181 tcgcgtgccc gcgcgctgtc tcgattggtc gccggaaatt agactggacg atggcctaga
      241 ggccacgatt gcattcttcc gggaacacgc caggtcacca gcctacgcgc tcaacccagc
      301 ggattgacgg ccgctcggca gcctgacggt caaggatcag ccgtcctgag attcctgctc
      361 ggccgcgagc gtctgagtcc gctggtaggc cgcatagacc gacttgctga gcaccgtgcg
      421 gaggccgcct ttctccatct ggtagatcgc ctctgccgac gtgccaccgg gcgaggtcac
      481 catgttgcgc agctcggcga ggtgccgccc ggagctccgc gcaaacgaga ccgatcctga
      541 cactgtctgc agcacgatct cctcggcgag atggcgcggg aagcccatgt gaacgcccgc
      601 gtcgacaagc gcctccatca tcaggaagac gtaggtcggg cccgtgccag agagcgccgt
      661 tgccatatcg acgtacttct ccgtctcgac ctcgatctcc tcgccgagcg ctgcgagcac
      721 cgaacgcact gcgtcgcgct gctcgccact cacggcctcg gtcgccgccc agaccgacat
      781 cccctcgctc acttgggcag gggtgttcgg catgacccgc accacggcgt catgccccaa
      841 cccgcgccgg agcgtcgaaa gcgccggccc ggcgatgatg gagatgacga cctgccgtgg
      901 gctgagctgg ccatgcagct cctgcatgac cttgcggagc acctgcggct tgatcgtcag
      961 caacaccaga tcggcgtccg cggccgcctc gcggttcgag gccgtcacct gcacgccata
     1021 ccgatccgcc agcctctcgc gccgatcggc gcgagggtgg ctggcaatga tctgcgccgg
     1081 tggcagcatc cccttcgcca gcaagccggc gatgatggat tccgccatca cgccggcgcc
     1141 cacgaacccg atgctcgtgg atgccagagc tgcttcggcc ttgcggccct tcttcttatc
     1201 gcccttgtcc tgcttcgtca cctgccgctc ccatcgctcg catcctggcc cacatcacct
     1261 gtcgtgacga gcatacggca ttgcgggtgc ggttacgcca cgggccgcga gcgcggctgc
     1321 gccgcggttg cttgcgggcg acccggctgg ccaaccatcc agtagaccaa tccgcccgca
     1381 ccgtacgtcg tggccaggaa cgccacgagg ggtccgaggg cagggatcag ggtcagcacc
     1441 tggagcagca acacgcccag cgcagcggcc agcatgagat tccggacatt agtcggccgc
     1501 agcagcagcg tcccgatctt ggcactcgcc accaggtagc cgaatagcca gagggccggg
     1561 atgaccacca cgaagaccgc cagtccgatc gggatgccga tcactgtcgc gaaggccagg
     1621 actgccagca gcggcagacc gatcagaagc acgagggcgg tcaggagtga tgcacccacg
     1681 cgttgctcca gcacctcgcc cgcagacgtc agcggccgac gaccgagcca cgcgaagagc
     1741 accgccgcga cgatcagcag cagcatgaag ccgatccaca tcatcacgga gaagacgaag
     1801 gcgccccatc cgaacgcagt caggtcgccc tcctcggaca ggtcgccagc gatgattgcc
     1861 ccttcagccc ggtcgagact gctgtcgaac agcgtcacgt tctcaacggt cgccgcctga
     1921 cccaggttca agtcggcatc gatcatgaca atgtcgccgc cgaccgtgcc gtccacggtc
     1981 gccgtaccac cgatgaccat gagaaaatcg gtcacctcgc cctcgacgac ggcactgcga
     2041 tcggccacga tgacggagct gatcgtttca tccagtccga tggtgacatc gccggtagcg
     2101 cgaaacagga acccgtcggt ctcgccacca tcggactggg ccgtaagcgc gaccatcggg
     2161 aacgtaagga ataccacaat cgagtggagc cgctgtgcaa gcgtggcacc gaccctgcga
     2221 gagcgtaagt ggagcgagca tcaagaagga gatgctcggc agcacaggcg cgacaggaaa
     2281 tgtgatacag gaggaagaca caatggaccg cgatctgaac cagtacgtcc cccgccaggg
     2341 catggatgtc tacggcgccg acaacgagaa gatcggcgac atcgatgccg tcgagcagga
     2401 atatttcgtc gtccgcaagg gctttttctt ccccgaggac cac
//
LOCUS       DASYWL010000186         2019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_459361, whole genome shotgun sequence.
ACCESSION   DASYWL010000186 DASYWL010000000
VERSION     DASYWL010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459361"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..501
                     /locus_tag="VGR22_06590"
     CDS             46..501
                     /locus_tag="VGR22_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128266.1"
                     /translation="MTQTNTNTYLWCDACRRSYRHHETRNGACPVCDRMTRPIGKYQA
                     IVRGLMSNELATSEIYTKHRQIIRMIWTRNGMGEQYYRALGADISYNRFEARVTDLLC
                     RGAREGWVSFEFPPAPTGDDATYRMEFDDEARFIDELTREFAEEIPSDA"
     gene            494..1120
                     /locus_tag="VGR22_06595"
     CDS             494..1120
                     /locus_tag="VGR22_06595"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HEV2128267.1"
                     /translation="MPESAFDRPPMRERTIGGYLDMLGSSDPTPGAGSAAGLLGALGA
                     SLGLMVISLTSSEDEGTSRTLDTARESLVRLRDRFTTLAEADERVFQGYRDAADMPKG
                     TPEEKRARKAAMQEALKQAASVPLKACETAVELARTIVPVQEHGNPFLLSDAQVAMLY
                     AATCFEASRVNVNVNLAMINDDAWITEVANHIQSLAADLRATSARVNP"
     gene            complement(1225..1641)
                     /locus_tag="VGR22_06600"
     CDS             complement(1225..1641)
                     /locus_tag="VGR22_06600"
                     /inference="COORDINATES: protein motif:HMM:NF024731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2128268.1"
                     /translation="MTRYQKRISGPLLDRIDIHLDVPRVDCEQLTDQRAGEASGGACE
                     GRGGSPGAGGAVRRHGVFHTNADMGPGEVQQFVRLGTAGEQLMAAAVRQLALSARAYH
                     RVLKLSRTIADLAGADGVEAARMAEALQYRPRHAEI"
BASE COUNT          385 a          676 c          631 g          327 t
ORIGIN      
        1 atcatgcatc ggggctgttt ccgttgaccg atgaggggcc atctcatgac ccagaccaac
       61 accaacacct atctctggtg tgacgcctgc cgccgaagtt atcgccatca tgagacgcgc
      121 aacggcgcct gccccgtctg cgaccgcatg acccgcccta tcgggaagta ccaggcgatc
      181 gtgcgtgggc tgatgtccaa cgagctggcc acgtcggaga tctacaccaa acaccggcag
      241 atcatccgca tgatctggac acggaatggc atgggcgaac agtactaccg agcgctcggc
      301 gcggacatca gctacaaccg cttcgaggcc cgcgtcaccg acctgctctg ccgaggcgcg
      361 agagagggat gggtttcgtt cgagttcccg ccggcgccga ccggtgacga tgccacctac
      421 cgtatggagt tcgacgacga ggcccgcttt atcgacgaac ttacccggga gttcgccgag
      481 gagatcccat ccgatgcctg aatcagcgtt cgaccgccca cccatgaggg agcgcacgat
      541 cggtggctat ctggacatgc tgggatcgtc ggacccgact cccggtgccg gcagcgcggc
      601 gggactcctc ggtgcgcttg gcgcgagcct tgggctgatg gtgatctcgt tgacgagttc
      661 agaggacgag ggaacgagtc ggacgctcga tacagctcgt gagtcactgg tccgactgcg
      721 ggatcggttc acgacactcg ccgaggcgga tgaacgcgtc tttcagggct accgggatgc
      781 cgccgacatg cccaagggca cacccgaaga gaagcgagcc cgcaaggcag ccatgcagga
      841 ggcgctcaag caggctgcca gcgtgcccct gaaggcctgc gagacggcgg tcgagctggc
      901 aaggaccatc gtgccggtcc aggagcacgg caacccgttc ctgctctccg acgcccaggt
      961 cgccatgctc tatgcggcta cctgtttcga agcgtcgcgc gtcaacgtca acgtcaacct
     1021 cgccatgatc aacgacgacg cttggatcac cgaggttgcc aaccacatcc agtccctcgc
     1081 cgccgacctc agagcgacca gcgcgagggt caacccgtag gaggaagcga agcggtcgaa
     1141 aatggaaccg tctcgtacgg gcagactcca tctccgcccc tttactatct tccttcctcg
     1201 aacggagaac ggaactcggt tccgctagat ctccgcgtgg cggggtcgat actgcagcgc
     1261 ttcggccatg cgagccgcct cgaccccatc tgcccccgcc aggtcggcga tcgtccgaga
     1321 cagcttcaac actcggtggt aggcccgtgc cgataacgcc agttgtcgca ccgccgccgc
     1381 catgagctgc tcaccggcag tgccgagccg gacaaactgc tgcacctccc ccggccccat
     1441 gtcggcgttg gtgtgaaaaa ctccgtgccg gcgaaccgct ccgcctgcac ccggcgagcc
     1501 tcctcgaccc tcgcacgcac ctcccgatgc ctccccggca cgctggtcag tcaactgctc
     1561 gcagtcgacg cgcggcacat cgagatgaat gtcgatccgg tcaagaagcg gcccgctgat
     1621 ccgcttctgg tagcgggtca ccgccgagga cgagcaggta caggcgcggg tctggtcccc
     1681 gaagtacccg ccgggcaggg attcatcgcc gcgaccagcg agacactcat cgaatcccgt
     1741 acgtacccgt gtgtaggagg ttgtccgagg acgaacgttc gggcgtcaga ggctgcggag
     1801 ggttgtccca ggatgccgct ggtgtgacct ccgtgtgacc ttgtacggcc gccaggaggc
     1861 caaggtcaca tcacgtatcc ggtgcggtcg atctcggagc tgaccaccca cgtcggcaag
     1921 gagtcgaacc ggctcgagga ggtcgaggcc ggcaccaagc atgacccgga cgaggtctgg
     1981 agcgagtacc gggacccgga ccgcgatccg tgggcaacg
//
LOCUS       DASYWL010000187         4133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_102587, whole genome shotgun sequence.
ACCESSION   DASYWL010000187 DASYWL010000000
VERSION     DASYWL010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102587"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1793
                     /locus_tag="VGR22_06605"
     CDS             <1..1793
                     /locus_tag="VGR22_06605"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEV2128269.1"
                     /translation="EIGIVLLLFAIGIQFSLGDLLHAGKTAAIGSTLQVILTIAVGFA
                     VGIAAGWEPLESLFFGAVLSNSSSTVLSKVLGERGEMDTGHGKLALAWSTVQDLSTII
                     LIVVLSALATGSDALLLDLGIEVGKAALFLVLLVPVGLRVLPWFFERIAELRSREVFV
                     LTVGVVALGLAYGATFFGLSLALGAFVVGVVVSESDLSHQILGEVLPLRDIFAGLFFV
                     SVGMLVDPGFVVRNVPLVLMTLAVIVLFKGALSATLAWLLGTPTRTAVLTGAVLAQAA
                     EFSFLLAQVGRDLGAISSTVFSLLLAAAAVSIVLLPAIHEASLPLADALHGRRSSFQP
                     DTVEHVAIPDTERLRGHAVICGFGRVGAVIGRVLRNRFTFVAIEEDVRIVSTLRSRGI
                     PVIRGNAAVPAILEQAHLERARELVIALADPVVTRQIVDQARHLNPRIRIIVRTHSEV
                     ERQWLLRRNVDVVVLGEWELALEMASHALRGFGVSAVETDVIIQRLRGQEDLESIRND
                     VTLPAPSRVGELIEPEPAPERTPAADEPITRRLHDAKGFRSESDVEEEHTSIRPAHDD
                     GTSPSRPTDSMVEQLSPEKQAAEIPASRER"
     gene            2781..3281
                     /locus_tag="VGR22_06610"
     CDS             2781..3281
                     /locus_tag="VGR22_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEV2128270.1"
                     /translation="MTDTSRSVATTRETRPRRWDPFAMFTELEAEMDRMFGQRLPAFL
                     PIRRYTGALGEGWAPSTDVFEKDGDIVVKAELPGVRKDDINVSVEQGSLVIKGERTAE
                     EEVKEENFYRSERFTGAFYRRFPLPQGVDEDKSTADYTDGVLEVHVPKPGGEAAKPEA
                     RKIQVS"
     gene            complement(3398..3913)
                     /locus_tag="VGR22_06615"
     CDS             complement(3398..3913)
                     /locus_tag="VGR22_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128271.1"
                     /translation="MYPQLTLRHPSHHRFGAEAVAAPIDVGGRRSVPEQARDVVILGL
                     NPTLRQTPQSITRIHSLYYSALWRGGDIVAVSDWLVAHWEEHAPPEPERQPDAVSLLG
                     GTGQGEGVRWYQRTSAICGYLPGSHHGCYCIPVADVPPTVPRLLHGGPSAGDRREEPS
                     TVMQQAGPAWQ"
BASE COUNT          662 a         1389 c         1319 g          763 t
ORIGIN      
        1 ccgagatcgg tatcgtgctg ctgctcttcg ccattggcat ccagttctcg ctgggcgacc
       61 tgctgcatgc cggcaagact gccgcaatcg gcagcacatt gcaggtcatc ctgacaatcg
      121 ccgtcggctt cgccgtcggc atcgcggcag gctgggagcc gctggagtcg ctcttcttcg
      181 gtgcggtgct ctccaactcg tcgagtacgg tactcagcaa ggtcctcggc gagcgcggtg
      241 agatggatac cgggcatggc aagctcgccc tggcgtggtc gacggtccag gacctcagca
      301 cgatcatcct gatcgtcgtg ctctccgcgc tggcgaccgg cagcgatgca ctgctgctcg
      361 acctcggcat cgaggtcggc aaggcggccc tcttcctcgt gctgctcgtg ccggtcggtt
      421 tgcgagtgct cccgtggttc ttcgaacgta tcgccgagct gcgaagccgc gaggtgttcg
      481 tgctgaccgt cggtgtcgtc gcgctcggcc tcgcctacgg cgccaccttc ttcggcctct
      541 cgctggcgct gggcgcgttc gtcgtcggcg tggtggtcag cgagtccgac ctctcgcacc
      601 agatcctggg tgaggtgctc ccgctgcgcg acatcttcgc ggggctcttc ttcgtctccg
      661 ttggcatgct cgtcgatccc ggattcgtgg tgcgcaatgt cccgctcgtg ctgatgacgc
      721 tggcggtgat cgtgctcttc aagggtgccc tttccgcgac gctggcctgg ctgctgggca
      781 caccgacccg aactgccgtc ctgaccggcg cggtactggc gcaggcggcc gagttctcgt
      841 tcctgctggc gcaggtcggc cgcgatctcg gcgcgatctc ctcgacggtg ttcagcctgc
      901 tgctcgccgc cgccgcggtc agcatcgtgc tgctgcccgc catccacgag gcgtcgctgc
      961 ccctcgccga cgccctgcac ggtcggcgat ccagcttcca accggacact gtggaacacg
     1021 ttgccattcc cgacaccgag cgattgcgcg ggcacgccgt gatctgtggc ttcggccggg
     1081 tcggagcggt gatcggccgc gtgctgcgca atcgcttcac cttcgtcgcc atcgaagaag
     1141 acgtccgaat cgtgtcgacg ctcaggagcc gaggcatccc cgtgatccgg ggcaatgccg
     1201 cggtacctgc catcctcgag caggcacacc tcgagcgcgc gcgcgagttg gtgatcgccc
     1261 tggccgatcc ggttgtgacg cggcagatcg tcgaccaggc gcggcatctc aacccgcgta
     1321 tccgcatcat cgtgcggacc cacagcgagg tggagcggca atggctactc cgccggaacg
     1381 tggacgtcgt ggtgctgggc gagtgggagc tggcactgga gatggcatcg cacgcgctgc
     1441 gcggcttcgg tgtgagcgcg gtggaaacgg atgtgatcat ccaacgcctg cgcggacagg
     1501 aagatctgga aagcattcgg aatgatgtca cgctacctgc gccaagccgg gtgggcgaac
     1561 tcatcgaacc cgagccggcg cctgagcgga caccggcagc tgacgagccg atcacccgcc
     1621 gcctgcacga tgccaaaggc ttccgttcag agagcgacgt ggaagaggag catacgtcga
     1681 tcaggccggc tcatgacgac ggcacatcac cttcgagacc gaccgactcg atggtcgagc
     1741 agctgtcgcc cgaaaagcag gccgccgaga tacctgccag ccgcgagcgg tgagctcggg
     1801 ctgcccctcc ccgtcaggat gggcgaacat tgggatttgc gggcccggtg tgaaggtggg
     1861 cattcaggcc cgtttctccc cgatcacttc ggtctcatgc gaaaaggtgc cgtcccagac
     1921 caagtagtca ctagagtcag ctcggtttcg tagtcgccct gccggaggct gatggggccc
     1981 tcgagcacga tatggtgctc cttccggggc tgggccggcg ggccgctgcc gttttcgctc
     2041 cgcagtgggt gccggcgggg cgctcggcgt cgtcgatgcc ccgaccaagg ccttgatctc
     2101 ctcgcaagcc agcgtctcct cgcgcacgag cgcctcggcc acccgctcca gctccacgcg
     2161 gtgagcgatc agcatcccta tgaccgcgat ccaaccgatc cacttggccg cgcttcgctt
     2221 ccgcccctca ggtgtcgctc tcgagtcgag atcgcgcatc ggtgccgcca tcagtcgtta
     2281 ttcaacaggc gtaccgcacc accggggcac caggaagtgc tgctgcgcca actcctccca
     2341 tcaagttggt gaaccgttca tcgacatgtg ttggggaacc ggttcggctg tgggtaagca
     2401 ccagggccac ggtgctccgc cgttcgaacg tccttcccgc gcacccttgg cgatgaagct
     2461 gggatgcgga aggtcccaaa gcgtctgttg gatgccctca gccgtctttg ctgacggtat
     2521 ttgtacttgc agtatgctcc tgccggcctg gcacgtgtaa ctcattcgcg gtcccgcatg
     2581 ttacaagttc gcatgaattt cgccgcgggt tgtagcgcag gcgttcgtca tcgcccagac
     2641 ccacgacagc ctgcaacgtt cttgcaacgc cgtgcccgta acttcggtca catttgcaat
     2701 cccccaattc ctacaatgga gccaccggac ccgaagtgga gtccggtggg ccgcacgttg
     2761 tcgaggaaag gagtcgcgcg atgaccgaca ccagccgcag cgtcgccacc acccgcgaga
     2821 cccgcccccg ccgatgggat ccgtttgcca tgttcactga gctggaagcc gagatggacc
     2881 gcatgttcgg ccagcggctg cccgccttcc tgcccatccg ccgctacacc ggcgccctcg
     2941 gcgagggctg ggcgccatcg accgatgtct tcgagaagga cggcgacatc gtcgtcaagg
     3001 ccgagctgcc cggcgtgaga aaggacgaca tcaacgtctc cgtcgagcag ggcagcctgg
     3061 tcatcaaggg cgagcgcacg gccgaggagg aagtcaagga agagaacttc tatcgttcgg
     3121 agcgcttcac cggtgcgttc taccgccggt tccccctgcc acaaggcgtg gacgaggaca
     3181 agagcactgc cgactacacg gacggtgtcc ttgaagtcca cgttcccaag cccggcggag
     3241 aggcggccaa gccggaggcc aggaagattc aggtcagcta gccctgatca gcgcgccgag
     3301 accgcctcgt ggcctcggcg ctggccattg cgccatgcgg tgcgagacct gagcgtgcga
     3361 cgctggctcc atgtggcata tgcctaccgc ctcacggcta ctgccacgca ggccctgctt
     3421 gctgcatcac cgtcgaaggc tcctcgcgac gatcaccggc gctcgggccg ccgtgcaata
     3481 accttggcac ggtcggtggc acgtccgcca cggggatgca atagcacccg tgatgggacc
     3541 ctggcaggta gccgcagatc gccgatgtgc gctggtacca gcgcacgccc tcgccctggc
     3601 cggttccgcc gaggaggctg accgcatcgg gttgccgctc aggctcgggc ggcgcatgct
     3661 cctcccaatg cgccaccagc cagtcgctca ccgcaacgat atcgccaccc cgccagagcg
     3721 cggagtagta caggctgtgg attcgggtga tgctctgcgg cgtctggcgc agcgtcgggt
     3781 tcagaccgag gatcacgacg tcgcgcgctt gctccgggac tgagcggcgc ccgccaacgt
     3841 caatcggtgc ggcgacggcc tccgctccga accgatggtg agatgggtgg cgcagtgtca
     3901 gttgcgggta cacagctcgt ctccttgtgt cattacgcca cgtccttcgt gtctatgacc
     3961 agcgtgacac cagcgagata gcattcgcca tatcgcccca ctcgaaatcg tgtcaatttc
     4021 gtgacaaacg gatcgttgag gtattgccat ggcttctccc atcaccctcg cgctcgccgg
     4081 agacgtcatg ctcggccgga tcgtcgaccg cgcagtagcc gagcatggac cgg
//
LOCUS       DASYWL010000188         4197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_252850, whole genome shotgun sequence.
ACCESSION   DASYWL010000188 DASYWL010000000
VERSION     DASYWL010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252850"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..291
                     /locus_tag="VGR22_06620"
     CDS             121..291
                     /locus_tag="VGR22_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128272.1"
                     /translation="MSAVVLAGSPNCPLVEAVAAHLAQHEAMLGTQDTNPRATEERSP
                     TPLGARMMYSHL"
     gene            392..916
                     /locus_tag="VGR22_06625"
     CDS             392..916
                     /locus_tag="VGR22_06625"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2128273.1"
                     /translation="MAFEPAPAGTHIGHVHLKVSDLERAVAFYRDLLGFEVMARYGTQ
                     AAFLSAGGYHHHLGLNTWESANGSPPPRGTTGLFHFAINYPERKDLAQALVRLLDGGW
                     GIDGASDHGTHEALYLHDPDFNGIELAWDRDPEAWPRHEDGSLDMYTKPLDFAGLLGN
                     ERAQAYAARLSVYR"
     gene            complement(1044..2843)
                     /locus_tag="VGR22_06630"
     CDS             complement(1044..2843)
                     /locus_tag="VGR22_06630"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF006006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEV2128274.1"
                     /translation="MSTRDPRRLNRRSLLKWGAAAAAVPAVAGSARLSSALMTDTQKS
                     VASSALQFPFPELEEITVAQVRSALDTRRISARELVDMYLARIEAIDRSGPELNSIIG
                     LNPEAQAIADAMDAELQRGQSRGALHGIPVLLKDNIDTADEMLTTAGSLALTESFPPN
                     DATVVQRLRDAGAIILGKTTLSEWANFRGFQSSSGWSGRGGQCLNPFVLDRSPCGSSS
                     GSAAAISANLALVSLGTETDGSIVCPSHNCGIVGIKPTVGLTSRNGVIPIAHNQDTVG
                     PHGRTVADAAAALGVLAGTDQRDPMTADATDKGHRDYTQFLDPNGLRGARIGVPRNVK
                     FFGYSREADVIIEAAIDALRNLGAEVIDPADFPHPDETLEAAATTEFEVLLYEFKADL
                     NAYLAARDDPEVGSLADLIEFNEMHFEEELIYFGQEIFLLAQEKGPLSEEAYLEALAK
                     NQRFSREQGIDAVLEEYNLDALVAPTGSPAWEIDLVNGDLFLGASSSPAAMAGYPLIT
                     VPAGFAFGLPVGMTFMGTAYSEPTLIKLAYAFEQATQVREAPRFLGAFEPTGVLGGIQ
                     GPFVPGIGTPVPVPLPSTPLPGAEATPTVEPAT"
     gene            complement(3307..3480)
                     /locus_tag="VGR22_06635"
     CDS             complement(3307..3480)
                     /locus_tag="VGR22_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128275.1"
                     /translation="MPDMPRTTREVLEDRLRLRKPGATVDDIAHNYADDLIVLTGTGG
                     ISGAAVFARLRAS"
     gene            complement(3619..>4197)
                     /locus_tag="VGR22_06640"
     CDS             complement(3619..>4197)
                     /locus_tag="VGR22_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV2128276.1"
                     /translation="VRVKDGVELSLRYATSVNQVRQKTQAVVKSSFEEIGIQTQLEQI
                     DAGIFFGGEAGNEQNINHFYWDLAMWTSGPASPIPTSFLEAWYAGPEDSLENVAQREN
                     QWQGQNFQRYVNEDYDALYEELLTVTDLERANEILIAMNDLLIEDVVIIPEVNRAADN
                     YAIDLRLEDANVAMSSFEYNYWNIANWVYRQG"
BASE COUNT          730 a         1399 c         1320 g          748 t
ORIGIN      
        1 ctggttcgcc gaccggttcg cggccctctg cacggagatg ggcaccgagg tcgatgatga
       61 cggaacacgc ctggtgattc gcccgcctgc ctttggccca cagaaaggga gtgcaacgcc
      121 atgagcgcgg tcgtcctcgc cggttcaccc aactgcccgc tggtggaagc cgtcgcggca
      181 caccttgccc agcatgaggc catgctcggt acgcaagaca cgaatccgcg tgccacagag
      241 gagaggtcgc cgacgccgct cggggcacgt atgatgtaca gccatctgta ggcatccaat
      301 tcggttggca tgactcaggt gactccgtag tatgtctacc agcaaggtag tttgtcttgg
      361 cacacaacct gtgttagaga ggaagcaccg catggcattc gaacccgcac cggccggcac
      421 gcacatcggc cacgtccacc tcaaggtctc cgatcttgag cgtgccgtcg ccttctaccg
      481 cgatctgctc ggcttcgagg tgatggcccg ctacggaacg caggcggcgt tcctctccgc
      541 cggtggctac caccatcatc tgggactgaa cacctgggag agcgccaacg gcagcccacc
      601 gccccgcggc acgactggcc tcttccactt cgccatcaac tatccggagc gcaaggacct
      661 cgctcaggcg ctggtgcgcc tgctcgatgg cggctggggc atcgacggcg cgtcggacca
      721 cggcacgcat gaggcgctct acctgcacga cccggacttc aacggcatcg agctggcctg
      781 ggaccgcgac ccggaggcct ggccacgcca cgaggatggc tcgctggaca tgtacaccaa
      841 accgctcgac ttcgccgggt tgctcggcaa cgagcgcgcg caggcgtacg ccgcccgcct
      901 cagtgtctac cggtagggat accgcctgcc atgagccagc ggagccgaca gggtgcgcca
      961 aggggcgtgc tcttttgaga acacgtccct tggctgcgct ggtatggaac cggccgggct
     1021 gcctgccccc ttgaccggcc cttctaagta gccggctcga ccgttggtgt cgcctcggca
     1081 ccggggagtg gtgtcgacgg gagcggtacg ggcaccggtg ttccgatgcc tggcacgaac
     1141 gggccctgta tcccgccgag cacccctgtc ggctcgaagg ccccaaggaa ccgcggtgct
     1201 tcccggacct gtgtcgcctg ctcaaaggca taggcgagct tgatcagtgt gggctcgctg
     1261 taggcggtgc ccatgaacgt catgcccacc ggcagcccga aggcgaagcc cgctggaacc
     1321 gttatcagcg ggtaccccgc catcgccgcc ggcgacgaac tggcgccgag gaagagatcg
     1381 ccattgacca ggtcaatctc ccacgccggg ctccccgtcg gtgcaacgag ggcgtcgagg
     1441 ttgtactcct cgagcacggc atcgatgccc tgctcgcggg aaaagcgctg gttcttcgcg
     1501 agcgcctcga gatacgcctc ttccgagagc ggtcccttct cctgcgcgag gaggaagatt
     1561 tcctgtccga agtagatcag ctcctcctcg aagtgcattt cgttgaactc gatgaggtcc
     1621 gcgagactac cgacttcggg atcatcgcgg gccgcgaggt aggcgttgag gtccgccttg
     1681 aactcgtaca gcagcacctc gaactcggtg gtcgccgccg cctcgagcgt ttcgtcaggg
     1741 tgggggaaat cagcgggatc gatgacttca gcgcccaagt tgcgcaaggc atcgatcgcg
     1801 gcctcgatga tgacgtcggc ctcgcggctg tagccgaaga acttgacgtt ccgcggcacc
     1861 cctatacgag caccccgcaa gccgttgggg tccaggaact gggtgtagtc ccggtgcccc
     1921 ttgtccgtgg cgtccgccgt catcggatcg cgctggtcgg tgccggccag caccccaagg
     1981 gctgcggccg catcggcgac cgtccgccca tgtgggccga ccgtgtcctg gttgtgcgcg
     2041 atagggatca cgccgttccg gctggtgagg ccgaccgtcg gcttgatgcc gacgatccca
     2101 cagttatgcg aggggcagac gatcgagccg tccgtctccg tgccgagcga gaccagggca
     2161 aggttggcgg agattgccgc tgcggatcct gagctggagc cgcagggcga gcggtcgagc
     2221 acgaaggggt tcagacactg cccaccgcga ccgctccatc cacttgacga ctggaagccg
     2281 cggaagttcg cccactcgct gagggtcgtc ttgcccagga tgatggcacc ggcgtcgcgt
     2341 agccgctgga caacggtcgc gtcgttcggc gggaacgatt ccgtcaacgc cagcgagccg
     2401 gcggtcgtca gcatctcgtc ggccgtatcg atattgtcct tgagcagcac gggaatgccg
     2461 tgcaacgcgc cgcgcgattg cccgcgctgc agctccgcgt ccatcgcgtc cgcaatagcc
     2521 tgcgcctcgg gattgagccc gatgatcgag ttgagttcgg gtccgctgcg gtcgatggcc
     2581 tcgatgcgtg ccaggtacat gtcgacgagc tcgcgtgccg agatgcgccg ggtatcaagc
     2641 gcggagcgca cctgggctac cgtgatctcc tccagttcgg ggaacgggaa ctgcagagca
     2701 gacgacgcga cggacttctg cgtgtcggtc atcagcgcgg atgacaggcg cgcactaccg
     2761 gcaacggctg gcaccgcagc tgcggcagcg ccccacttga gcagcgaacg gcgattcaac
     2821 cggcggggat cacgcgtact catcagtagc cctcctcgcg acacacgcga cgaacagtgg
     2881 gttgtgctcc aaggggtgga gtccccgcat catagcagcg gcctggaccg atcgcaactc
     2941 ggcgccgccg tgcccgctgc tcgagcccca acgctccagt aaccaggccc aaccatccct
     3001 tgatatggtg caatctcctc gatagccgct gtcaacgaga tcgcactacc cgaggatcga
     3061 ctccgcatga ccacgccacc actcgtgcat aacctgacct ccccggatgc ctacccatgg
     3121 cgaccagacc gggtcgaact gatcgagacc cacatctcgt gggtcttcct tgccggtgac
     3181 cgcgtcgtca agatcaagcg ctcggtcaaa ctgcccttcg tcgagcaaac ggccgtcgag
     3241 gcccgacgac gacgccctgc accaacttgt tgtgatactc gaagcgccct ttcggcaggt
     3301 actcgttcag gatgcgcgca gtctcgcgaa caccgccgcg cccgagatgc cccctgtgcc
     3361 ggtcagcacg atcaggtcgt ccgcgtaatt gtgcgcgatg tcatcgactg tcgcgccggg
     3421 cttgcgcagg cgcaaccgat cctcgaggac ctcacgggtg gtgcggggca tatccggcat
     3481 ggcaagatct cctgaaccag tagcgtgccg tcaatgctcg tggcccctca acccgcgttc
     3541 ccgcacaaga agaactgtcc aggcaccggt ttccggtgcc tggacagttg gaactcgtca
     3601 gctaagccgc ggctgggatt acccctgccg gtacacccag ttcgcgatgt tccagtagtt
     3661 gtactcgaac gagctcatcg ccacgttcgc gtcctccagc ctgagatcga tcgcgtagtt
     3721 gtcggcggcg cgattgacct ccgggatgat cacgacatcc tcgatgagca ggtcgttcat
     3781 ggcgatgagg atctcgttgg cccgctcgag atcagtgacc gtgagcagct cctcgtacag
     3841 cgcgtcgtag tcctcgttga cgtagcgctg gaagttctgc ccctgccact ggttttcccg
     3901 ctgggcgacg ttctccagcg agtcctccgg cccggcgtac cacgcctcaa ggaaggaggt
     3961 gggaatcggg ctggcgggac cgctcgtcca catcgccagg tcccagtaga aatggttgat
     4021 gttctgttcg ttgccagcct caccaccgaa gaagatgccc gcatcgatct gctccagctg
     4081 ggtctggatg ccgatctcct cgaaggagct cttgaccacg gcctgcgtct tctggcgcac
     4141 ctggttgacc gaggtcgcgt agcgcagcga cagctcgacg ccatccttga cccggac
//
LOCUS       DASYWL010000189         3295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_515189, whole genome shotgun sequence.
ACCESSION   DASYWL010000189 DASYWL010000000
VERSION     DASYWL010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515189"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="VGR22_06645"
     CDS             <1..882
                     /locus_tag="VGR22_06645"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEV2128277.1"
                     /translation="AVSSAMLVGSGSYRAALAQATPPGDDYARPEALVDVAWLQKNMD
                     APDLVTVGFMPVEEFNIAHIPGSARIAAPELEVIDTSDASIEQWQQTTRQLLGDIGIS
                     SASTVVVYDPGTLFAARLWWILHYFGHENVHILDGGLPAWQEAGHEVEEGLAEAPAGS
                     EPYQGTPNADLLAQMDEVMANLEVANAVILDARKLEEYTEGHIPGAVNINFPLNAAPE
                     PPKFWKPASELKALYEEAGVTPDKLVIPYCASGVRSAVTAFSLHLIGYEQLALYTGSW
                     LEWGEDPDTPKTTGDQP"
     gene            complement(1902..3035)
                     /locus_tag="VGR22_06650"
     CDS             complement(1902..3035)
                     /locus_tag="VGR22_06650"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEV2128278.1"
                     /translation="MRIREIRAVGLYGATPEGGWSHELRPEDSVHTLIAVSTDEGLTG
                     LGSVFTYVGLVKAALRVLEPIYLGENAIEPERVSEKLHQHTFWLGRGGSLTHTISGID
                     IALWDLLGQVTGQPVGRLLGGRYRERVRPYASLLMDEPESLTEHLHATKAQGFRAFKI
                     GWGPFGRRSNRLDETIVRAARDTVGPDALLMVDAGASDAFWPQGYKWALRTATMLAAY
                     DVAWFEEPLPPDGLEDYANLRRAVPVPIAGGEVLTRRQSFQPWLEGRAFDIVQPDVTK
                     VGGISEERRIGWMAQDHGVRLIPHGWNTALGLAADLQLASALPGTDLVEYLTCSPYVD
                     EIVEEGWQLDADGLLPIPEAPGLGIRLNLDAVGRYAGGERLLG"
BASE COUNT          605 a         1097 c         1045 g          548 t
ORIGIN      
        1 gcggtgagct ccgccatgct ggtcggctcc gggtcgtacc gggccgcact ggcccaggcc
       61 acgccgccgg gtgacgacta cgcccggcct gaggcgctgg tcgacgtcgc ctggcttcag
      121 aagaacatgg acgcccccga cctcgtcacc gtcggcttca tgcccgtgga ggagttcaac
      181 attgcccaca tcccgggatc tgcccggatc gccgcgcccg agctggaagt gatcgacacc
      241 tccgacgcct ccatcgaaca gtggcagcag acgacgcggc agctgctggg cgacatcggc
      301 atctcgtcgg cgagcaccgt ggtcgtctac gaccccggca cactcttcgc cgcccggctc
      361 tggtggatcc tgcactactt cggccatgag aacgtccata tcctcgacgg cggtctccct
      421 gcctggcagg aggctggcca tgaggtcgag gaaggtctgg cggaggcgcc tgccggtagc
      481 gagccctacc agggcacgcc gaacgccgat ctgctcgccc agatggatga agtcatggcc
      541 aacctcgaag tcgcgaacgc cgtcatcctc gacgcgagga agttggagga atataccgag
      601 gggcacatcc ccggcgcggt caacatcaac ttcccgctca acgcggcgcc ggagccgccg
      661 aagttctgga agccggcgag tgagctgaag gcgctctatg aggaggcggg cgtcacgccg
      721 gacaagttgg tcatccccta ctgcgcgtcc ggtgtgcggt cggccgtcac cgcgttcagt
      781 ctccacctga tcgggtacga gcaactcgcg ctctacaccg gctcctggct ggagtggggc
      841 gaggacccgg atacgccgaa gactacaggc gaccagccat agccggctga gccggtagtt
      901 cacccacact ctcgctcctg gtccgagcga cgccgtccgc aacgacggtc cgaccgcgta
      961 gtgctgggag agcggccgga ggccccaagg attacatcgt cgtgtcgtca cccttgtcct
     1021 tcctgaacag ggagccagtc tagcagtact cgcaccctag gaggccacgg tcggtcacat
     1081 gaaagcgacg gagagcccgg accagtaacg gtccgggctc tcttccaggg cagccggcga
     1141 acgcggctcg atgcgaactg gcgagaccgg cttggcgatg gtccccgatc aaccctggaa
     1201 gcggcttcac accgtcgacc tcggtcgcac cgctctggag aaggtagctg gtgggcaggc
     1261 tggtgatcag ccgttccagc gcgaccgcat cccgctcagc atgcgctgcc actcgtgcgc
     1321 cacggcgatc ggatctcggt accgcttgga agagtcggcg agcgaatcga agatcaggcg
     1381 tcggcggtga cgcgccggcg cgcattgtac tccgggtgct catgccagct tttccgagca
     1441 ttcgcccgct tgcggcgggt gcggcaatcc gtacagtagc cccgtcgccg ccggcagggg
     1501 tacggctcgc cgcagtctgg acacacatgc actccttcgg cactcaccag gacgtcgatc
     1561 aggcgctcca caatgtctgc gaagatgctg tccggaagga ggaaggcaga catctcggtc
     1621 atcggtcatc attgaagggc cgatggtcgc gcgcggccgg tccccaaagt gagcgattca
     1681 tgaggcagag cgatcgctcg atctcgcgcc cttggtaacc gcgaccggag caggagcggg
     1741 gtataaccga aatacgtgga ggagctcgac cgcgcccgtt cagcggcacg cctgattgcc
     1801 cctccccacg cgccagcgaa gatcgcacgg attgagctgc actgcacgat tcagacatct
     1861 gaagccgagt gcggcaatgc agggttcggt tgcggctggc atcaccctag caagcgctca
     1921 ccgccggcgt agcgcccgac agcatcgagg ttgaggcgaa tcccgagacc aggagcctcg
     1981 gggatcggca gcaggccgtc agcgtcaagc tgccacccct cctcgacgat ttcatcgacg
     2041 tacggcgagc atgtcaggta ctcgacgagg tcggtgccag gcaaggcgga ggcgagctgg
     2101 aggtcggcag ccagtccaag ggccgtgttc cagccgtggg gaatcagccg gacgccgtgg
     2161 tcctgcgcca tccacccaat ccggcgttcc tcgctgattc caccgacctt cgtgacatca
     2221 ggttggacga tgtcgaaagc ccgcccctca agccagggtt ggaaggactg gcgacgtgta
     2281 agtacctccc caccagcgat cggcacggga acggctcggc gaagattggc gtaatcctca
     2341 agtccgtccg gtgggagcgg ctcctcaaac cacgcgacgt cgtaagcagc gagcatcgtt
     2401 gctgttcgaa gcgcccactt gtacccctgt ggccagaagg catcgctggc accggcatcg
     2461 accatcagga gagcatcggg tccgacggta tcccgtgcgg cgcgaacgat cgtctcgtca
     2521 aggcgattgc tgcggcggcc gaagggtccc cagccgatct tgaaggcccg gaacccttgc
     2581 gccttggtcg catggagatg ctcggtgagc gactctggct catccatgag caacgaggcg
     2641 taaggccgaa cacgttctcg atagcgaccg ccgaggagcc ggccaaccgg ctgtccagtc
     2701 acctgaccga gaagatccca aagcgcgatg tcaattccgc tgatggtgtg cgtcagtgac
     2761 ccaccccgcc ccagccagaa tgtgtgctgg tggagcttct cgctgactcg ttcgggctca
     2821 atggcgttct cgccaaggta aatcggctcc agcaccctga gtgccgcctt caccagtccg
     2881 acgtacgtga agacactgcc gaggccggtc aggccctcgt cggtgctgac ggcaatcagc
     2941 gtgtgcacgc tgtcctcggg gcggagttcg tggctccacc cgccttcggg cgttgcaccg
     3001 taaagtccga ccgcgcgaat ctcgcgaatt ctcaccgcgt gtccctcttg atctcaaacg
     3061 caacggcatg catggatgcg gtggcactct cgcaccaggg accaccagcc caatcaatcg
     3121 acatcagatg ctgtcaccag cgaatctcgg actggattgg gttggtatgg aagtcgcgtc
     3181 gcccggcgac cggccaggcg cgccactcct cgacgaactc cagcaggcca gccactgccg
     3241 cctcgcagat caccttgccc ttttcagccg tcgcggtccg tgggtcaccg tgcac
//
LOCUS       DASYWL010000190         4945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_627894, whole genome shotgun sequence.
ACCESSION   DASYWL010000190 DASYWL010000000
VERSION     DASYWL010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627894"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2654
                     /gene="uvrA"
                     /locus_tag="VGR22_06655"
     CDS             <1..2654
                     /gene="uvrA"
                     /locus_tag="VGR22_06655"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEV2128279.1"
                     /translation="QKGTSRNPRSTVGTVTEIYDYLRLLYARIGHPHCPNCGRPITQQ
                     SVQQMADQVLTQRDGTRLMILGPVIRDRKGEHAAVLDEIRRQGFVRVRVDGEIRDLDE
                     DIKLAKTKKHTIEVIVDRIIIRHDDPNEIDIEGHPDRIRLIDSLESALRMSDGIAKVQ
                     IIDGEELTFSEHFACEYCGISIGELEPRNFSFNSPHGACPECTGLGTKMEFDPDLIVP
                     NKNLSIAQGAIVPWMRSGFENSSWYSALLRAASETFGFSLEVPVRELSDEALDIVLNG
                     TKGKKISIRYESKRGRERTYSMPYEGVVPNLRRRYGQTQSDYMREEIERYMTARPCPV
                     CEGARLKPEVLSVLIRGNSIVDVTRQPIRVALSWFTEMSDDPSENPSAPLSMREFYIA
                     RQILKEIRERLGFLVDVGLDYLSLDRAANTLSGGEAQRIRLATQIGSSLMGVLYILDE
                     PSIGLHQRDNNRLIRTLERLRDLGNTLLVVEHDEDTMRAADWIIDIGPGAGEYGGELV
                     AEGPVGAIEQAEGSITGQYLSGKREIEVPEERRAGKGNRLRLEGAKENNLRDISVEFP
                     LGTFTCITGVSGSGKSSLITDTLYRRLAQELHGAKVKPGAHEALEGVEHLDKVIEIDQ
                     SPIGRTPRSNPATYTGVFTPIRELFASMPEAKTRGYKPGRFSFNVKGGRCEACKGEGI
                     IQIEMNFLPDVFVPCEVCKGKRYNREALEILYKGKSISDVLDMTVEEAVAFFQNIPAI
                     RRKLATLNDVGLGYVKLGQPATQLSGGEAQRVKLASELSRRATGKTLYILDEPTTGLH
                     FADIERLLGVLQRLADGGNTIIVIEHNLDVIKSADWIIDLGPEGGSGGGELIAEGTPE
                     EIARSPKSHTGAYLAPALGIPSAAAAD"
     gene            2866..3405
                     /locus_tag="VGR22_06660"
     CDS             2866..3405
                     /locus_tag="VGR22_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEV2128280.1"
                     /translation="MRDSVLVLNQNYEPLNVCSVRRALNLVLGGKAELLEAARGRIAS
                     ATRAFPFPSVIRLVHLIKRPRPRVKLTRREIFIRDNHTCQYCGVRGTKLTIDHVIPRS
                     RGGLHTWDNVVTACQPCNHRKGGKSVAEARMVLQMEPREPRAGQYYTIERRINAALDE
                     AWSKFLPGLSPLPSSTQSA"
     gene            3519..4829
                     /gene="serS"
                     /locus_tag="VGR22_06665"
     CDS             3519..4829
                     /gene="serS"
                     /locus_tag="VGR22_06665"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEV2128281.1"
                     /translation="MLDIQLIRDNPEVVREASRQKNVDAPVDEILRLDTRRREFVTQV
                     DRLKADRNAGSKLVGKTKDLEERNRRIAEMRELGDRIDALDGEIRTVDEQLRELMLTI
                     PNIPDPDVPVGSDETANEVGPTHGRIREFDFGPLPHWDLAEHLGIIDFERGVKVAGAR
                     GYVLRGDGSRLQRALITWMLDVHIREHGYSEVTPPYMVLGEMLVGTGNLPKFAETLYK
                     DRYDDKWLIPTAEVPVTNLYRDEILSEDQLPIYHVAHTPCFRREQISAGRDVRGIKRV
                     YQFDKVEMVKFVHPDASPEELDRLVREALDIVERLDLPYRILDLSTGDMSFASAHTYD
                     IETYAPGSEEWLEISSCSNMRDFQARRMNIRYRPEEGKRPQLLHTLNGSGLALPRTII
                     AIMENYQTVDREIIVPEALRPYMGGQERIDVQPPIGAAESPIEV"
BASE COUNT          937 a         1637 c         1557 g          814 t
ORIGIN      
        1 accagaaggg cacctcccgc aatccccgct ccacggtcgg gaccgtcacc gagatctacg
       61 actacctgcg actcctctac gcccggatcg ggcacccgca ctgcccaaac tgcggtcgac
      121 ccatcaccca gcagtcggtt cagcagatgg ccgaccaggt gctcacccag cgggatggta
      181 cacggctgat gatcctcggc ccagtgatcc gcgatcgcaa gggcgagcac gccgccgtgc
      241 tggacgagat tcgccggcag ggttttgtcc gcgtgcgggt cgacggcgag atccgtgatc
      301 tcgacgagga catcaagctc gcaaagacga agaagcacac gatcgaggtc attgtcgacc
      361 ggatcatcat tcgccacgac gacccgaacg agatcgacat cgagggccat ccggaccgca
      421 tccggctcat cgactccctg gagtccgcat tgcgcatgtc ggatggcatc gccaaggttc
      481 agatcatcga cggcgaggag ttgacattct cggagcactt cgcctgcgag tactgcggga
      541 tcagcatcgg cgagctcgag ccgcgcaact tttcgttcaa ctcgccgcac ggcgcctgcc
      601 ccgagtgcac tggcctcggc accaagatgg agttcgatcc ggacctcatc gtgccgaaca
      661 agaacctgag cattgctcag ggcgccatcg tgccgtggat gcggagcggg ttcgagaact
      721 cgagctggta ctcggcgctg cttcgggccg ccagcgaaac gttcggcttc tcgctcgagg
      781 tgcccgtccg cgagctgtcc gacgaggcgc tcgacatcgt gctcaacggc acgaagggca
      841 agaagatcag catccgttac gagtcgaagc gcggacggga gcggacctat tcgatgccct
      901 atgagggggt cgtcccgaac ctgcgccgac gatatggcca aacgcagtcc gactacatgc
      961 gcgaggagat cgagcggtac atgaccgccc ggccctgccc ggtctgcgag ggcgcgcgac
     1021 tgaagccgga ggtgctctcc gtcctgatcc ggggcaactc catcgtcgac gtgacccggc
     1081 agccgatccg cgtcgcgctc tcatggttca ccgagatgag cgatgacccg tccgagaacc
     1141 cgagcgcgcc gctctcgatg cgcgagttct acatcgcccg gcagatcctc aaagagatcc
     1201 gcgagcgtct cggcttcctc gtcgacgtcg ggctcgacta cctctcgctc gaccgcgccg
     1261 cgaacacgct ctctggcggc gaggcgcagc gcatcaggct ggcgacccag attgggtcga
     1321 gcctgatggg agtgctctac attctcgacg agccgagcat cgggctccac caacgggaca
     1381 acaaccggct gatccgtacg ctggagcgac tgcgcgacct cggcaatacc ctcctcgtcg
     1441 tcgagcacga cgaggacact atgcgcgcgg ctgactggat catcgacatc ggtcccggtg
     1501 ccggcgagta cggcggcgag ctggttgccg agggtccggt cggggccatc gaacaggcgg
     1561 aggggtcgat caccggccag tacctcagcg gcaagcggga gatcgaggtc cctgaggagc
     1621 gccgcgcggg caagggcaac cggctgcggc tcgagggcgc gaaggaaaac aatctccgcg
     1681 acatctccgt cgagttccca ctcggcacct tcacctgcat caccggtgtc tccggttccg
     1741 gcaagagctc gctcattacc gatacgctct accgccggct cgcgcaagag ctgcacggcg
     1801 caaaggtgaa accgggcgcc cacgaggcgc tggagggcgt cgagcatctc gacaaggtga
     1861 tcgagatcga tcaaagcccg atcggaagga cgccgcgctc caaccccgcg acctacacgg
     1921 gtgttttcac accgatccgc gagctgttcg catccatgcc agaggcgaaa acccggggct
     1981 acaagccagg ccggttctca ttcaatgtca agggcggccg ctgtgaggcg tgcaagggcg
     2041 agggtattat ccagatcgaa atgaactttc tgccagacgt cttcgtgccg tgcgaggtct
     2101 gcaagggcaa gcggtacaac cgcgaggcgc tggagatcct ctacaagggc aagtcgatct
     2161 cagatgtcct cgacatgacc gtcgaggagg cggtcgcctt cttccagaac atcccggcga
     2221 tccgacgcaa gctcgcaacg ctcaatgacg tcggtcttgg ctacgtgaag ctcggccagc
     2281 cggcaacgca gctgtccggc ggtgaggcgc agcgcgtcaa gctcgccagc gagctctccc
     2341 gtcgcgcgac cggcaagaca ctctacatcc tcgacgagcc gacgaccggg ctgcacttcg
     2401 ccgatatcga gcgcctgctc ggcgtcctgc agcggctggc cgacggcggc aacacgatca
     2461 ttgtgatcga gcacaacctc gacgtgatca agtcggccga ctggatcatc gacctcggtc
     2521 cggagggcgg ctcgggcggt ggcgagctca tcgccgaggg gacgccggag gaaatcgcac
     2581 gcagcccgaa gtcgcacacc ggtgcctacc tcgctccggc cctcggcatc ccctcggccg
     2641 ctgccgccga ctagggccgg cctcaactga cgcagctgga agggcggacg ctagggaacg
     2701 ctcgccctat cggtagcgcg cgggcagcac gcttcataat ctgttgatcc tcaacgttgt
     2761 agggccgccg tcctgcgtgc taccatcgct tctgacaggt gtcggttgcg tcagacatcg
     2821 acgggtgtgt cggatcgtcg tagccggcca cggaggttcc gcagcatgag ggattccgtc
     2881 ctggtcctga accagaacta cgagccgctc aacgtctgca gtgtgcgaag agcgctcaat
     2941 ctggtgctcg gcggcaaggc cgagcttctc gaggcagctc gcggccgtat cgcgagtgcc
     3001 acccgcgcgt ttccattccc gtcggtcatc cgcctggtac acctcatcaa gcgtccgcgg
     3061 ccccgcgtga agctcacccg cagggagatc ttcatcaggg acaaccatac ctgccagtat
     3121 tgcggtgtac gtggcacaaa actgacgatc gaccacgtca ttccccgctc gcgcgggggt
     3181 ttgcatacgt gggacaacgt ggtcaccgcc tgccagccct gcaatcaccg gaagggcggc
     3241 aagagcgtgg ccgaggcccg catggtgctt cagatggagc cgcgggagcc gcgcgccggc
     3301 cagtactaca ccattgagcg gcgcatcaat gccgcgctcg acgaggcgtg gtcgaagttc
     3361 ctccccggcc tctccccact gccttccagc acacagtctg cctgagcaac ggccggcgtc
     3421 ccgtttcgcg gcgaccggcg acgttgctcg ttgatccggc tgccgtgtgg cagagttggt
     3481 ctggatagac caatctgctc tcctggaagg taacgactgt gcttgacatc cagctcatcc
     3541 gggacaaccc cgaagtggtg cgcgaggctt cgcgccagaa gaatgtcgac gctccggtcg
     3601 acgagattct ccggctcgat actaggcggc gcgagttcgt cacgcaggtt gaccggctca
     3661 aggccgaccg caacgccggc tcgaagctcg ttggcaagac aaaggacctc gaagagcgca
     3721 accggcgcat tgccgagatg cgcgagctcg gcgacaggat cgacgcgctc gacggggaga
     3781 ttcgcacggt cgacgaacag ctgcgcgagc tgatgctaac cattcccaac atcccggatc
     3841 ctgacgtgcc ggtcggatcg gacgagaccg cgaatgaggt cgggcccacc cacggcagga
     3901 tccgggaatt cgacttcggc cccctgcccc actgggatct cgccgagcat ttgggcatca
     3961 tcgacttcga gcgcggggtg aaggttgccg gcgcgcgagg ctacgtgttg cgcggcgacg
     4021 gctcgcggct ccagcgggcg ctgatcacgt ggatgctcga cgtgcacatc cgggagcatg
     4081 gctacagcga ggtcacacca ccctatatgg tgctcggcga gatgctggtc ggcaccggca
     4141 acctgccgaa gttcgccgag acgctctaca aggaccgcta cgatgacaag tggctgatcc
     4201 ccaccgcgga ggtgcccgtc accaacctgt accgagacga gatcctgagc gaggatcagt
     4261 tgccgatcta tcatgtcgcc cacacgccct gcttccgtcg agagcagatc tcggccgggc
     4321 gagatgtgcg cggcatcaag cgggtctacc agttcgacaa ggtcgagatg gtcaagttcg
     4381 tccaccctga tgcctcgccg gaggagctcg accggctcgt tcgcgaggcg ctcgatatcg
     4441 tcgagcgcct cgacctgccc taccgcatcc tcgacctctc gacgggggac atgtccttcg
     4501 cctcggcgca cacctacgac atcgagacgt atgcgccagg atcggaggag tggctggaga
     4561 tctcgtcgtg ctcgaacatg cgcgacttcc aggcccgtcg aatgaacatc cgataccgtc
     4621 cagaggaggg caagcgcccg caactgctgc acaccctcaa cggctcgggc ctcgcgctgc
     4681 cgcgcacgat aatcgcaatc atggagaact accagaccgt cgaccgcgaa atcatcgttc
     4741 ccgaggcatt gcgcccctac atgggcggcc aggaacgcat cgatgtccag ccgccaatcg
     4801 gtgccgcgga gagcccgatc gaggtgtagg cgcttctagg tctccatgag gcgacgctgt
     4861 cttgtccacg gtggtgccct ctagccgcac tgtgtgttgg accacacgca gtcgccggtc
     4921 gagcgcaggc acgaccggcg actgc
//
LOCUS       DASYWL010000191         2197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_628002, whole genome shotgun sequence.
ACCESSION   DASYWL010000191 DASYWL010000000
VERSION     DASYWL010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628002"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(300..1184)
                     /locus_tag="VGR22_06670"
     CDS             complement(300..1184)
                     /locus_tag="VGR22_06670"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEV2128282.1"
                     /translation="MTPSTAGKSVRTIWFGMRCAFTRAALEGAIAAGDAVTPVAAVLP
                     RGPKPASAGWPEPPLDRWLRERGIEIVEVGRLTEVDLAAIERLVREREIPLGAGACFP
                     WKVPAQLRSAIRCGVLNIHPSLLPALRGPEPVFHAYRLGLEATGVTVHLMDDGWDSGP
                     IVAQTRVAIPDDGRADAFEAELARRGGEMLAGIAHQWCAGTLDARPQDDARASWAPVP
                     GPTERHMLRTLTVAQARRFLSACPPLTATDADTGVTVAVAGVAARGEVAVRVRCRDGD
                     LWVHRIGTNGVANGTGGV"
     gene            1281..1586
                     /locus_tag="VGR22_06675"
     CDS             1281..1586
                     /locus_tag="VGR22_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEV2128283.1"
                     /translation="MPRMVHCVKLGKELPGLEKPPFSGELGQRIYENVSQEGYDLWKP
                     HMTILINHYALNPADPETRKLLRKEMEEFFFGENAQLPEGWTPPSAAPTKGGAPPRK"
     gene            complement(2015..>2197)
                     /locus_tag="VGR22_06680"
     CDS             complement(2015..>2197)
                     /locus_tag="VGR22_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase"
                     /protein_id="HEV2128284.1"
                     /translation="GIKITAPCNQPIVPLPEGESYLGFIFAEGETAADAERALRSAHE
                     RLSFRIDPTIPLMRRS"
BASE COUNT          403 a          759 c          699 g          336 t
ORIGIN      
        1 tgccgtctgc aaacagagcg gcacccggcc agccatggac cgggaacatc ctgacgttga
       61 tgtagtgtgt gatggaaacg acgatcgccg gccctctctc gacgatggga agtggcattc
      121 gagccgaatc cgggaggggc catcccccgc tgcccggact catgaacctg gatcgtgcct
      181 gctggaatgg accagtgtgc cgaacccgaa gcgcatctcg cgacaggccc cagccaaggc
      241 gctctatcat gcagatgcgg gttctgacac acgccttatc gcatacttgc gaccgcttgt
      301 caaacgcctc cggtaccgtt cgcgacgcca ttcgtgccta tacgatgaac ccagaggtca
      361 ccatcccggc agcggacacg caccgccacc tcgccacgag cggcaacacc agccaccgcc
      421 accgttaccc cggtgtcggc gtccgtcgcg gtcagcggcg ggcaggcgga cagaaagcgc
      481 cgtgcctgtg cgacagtgag tgtgcgcagc atgtgtcgct cggtggggcc gggcaccggt
      541 gcccagctcg ccctggcgtc gtcctgcggg cgtgcatcga gcgtgccggc gcaccactgg
      601 tgtgcgatgc ctgcgagcat ctcgccaccc cgccgggcga gctcggcctc gaaggcgtca
      661 gcccgtccgt cgtctgggat cgccacccgc gtctgagcga cgatcggacc gctgtcccag
      721 ccgtcgtcca tcaggtgcac ggtgacgccg gtcgcctcca gtcccaagcg gtaggcgtgg
      781 aagaccggct ccgggccgcg cagcgccggc aacagcgacg gatggatgtt gagcacgccg
      841 cagcgtatgg ccgatcgtag ctgggccggc accttccatg gaaagcacgc gccggccccg
      901 aggggaatct ctcgctctcg caccagccgt tcgatcgcgg cgagatccac ctcagtgagc
      961 cgacccactt cgacgatctc gatccctcgc tcgcgcagcc agcggtcgag gggaggctcc
     1021 ggccacccgg cactcgccgg cttggggccg cgtggcagca cggccgccac cggcgtgaca
     1081 gcatcaccag ccgcaatcgc cccctccagc gccgcccgcg tgaaggcgca ccgcatgccg
     1141 aaccagatcg ttcggaccga cttgccggca gtggatggag tcaccggcac cgcgcacccc
     1201 ctctgctaca ctggcgtaga cgaaccgggc atccgcgcgg tgcccgcgta cccgtgagtc
     1261 atcggacagg gagacccgac atgccccgca tggtgcactg cgtcaaactc ggcaaggaac
     1321 tgcccgggct ggagaagccg ccgttctcgg gggagctcgg ccagcgcatc tacgagaacg
     1381 tctcccagga gggctacgat ctctggaagc cccacatgac gattctcatc aaccactatg
     1441 cgctgaatcc ggccgatccg gaaacccgca agctgctgcg caaggagatg gaagagttct
     1501 tcttcggcga gaacgcgcag ctgcctgagg gctggactcc tccctccgct gctccgacca
     1561 agggtggcgc cccaccgcgc aagtagcgag taagcagaag aagggtgcgc cccgtcaggg
     1621 gggctgtccg tccttcgcgg caacctgcca aaatccctcg agaccaaaca tgactgtcat
     1681 gctgagccgg cgaggcgcac cggcgccgaa gccgggtcga agcatccccc gtccggccta
     1741 tggccgggcg gtctgttttc cggtggcaat ctatttcaga agcacgctga cagcatgcag
     1801 cgaaaaaacc ggcgctgagc gccggggata cttcgcagag tttatcctga gcgaagccga
     1861 agggctcaga atgacagcca tgtggttggc tcttgaaagc cgatggcacc aggacaccaa
     1921 cggccatccg ttgagaggtg ggcctccagg ttggctcgca cgagcatgaa tcgagacaat
     1981 gctcggcggg acggcggtcg acatcgctgg acgcctacga cctccgcatc aggggaattg
     2041 tcgggtcgat gcggaaggac agccgctcgt gggccgatcg caacgcccgc tcggcatcgg
     2101 ccgccgtctc cccctcggca aagatgaacc cgagatagct ctccccttcc ggcagcggca
     2161 cgatcggctg gttgcacggc gctgtgatct tgatccc
//
LOCUS       DASYWL010000192         3485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_83689, whole genome shotgun sequence.
ACCESSION   DASYWL010000192 DASYWL010000000
VERSION     DASYWL010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83689"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VGR22_06685"
     CDS             <1..300
                     /locus_tag="VGR22_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128285.1"
                     /translation="PPDEIVGTEDASHAPAARLRIHVAISPEDSAWGGQVAHEVVTQH
                     AGTNDVVRVFFHASAEVVDPGELARVTETAHAAAGAYRVPLGPLTEAEEADQRGG"
     gene            689..1486
                     /locus_tag="VGR22_06690"
     CDS             689..1486
                     /locus_tag="VGR22_06690"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV2128286.1"
                     /translation="MDWGANKLNRHSPVPLYYQLVEHIRKSVRIGQLGTNAKLPAERD
                     IAEQAGVSRMTVRQALTFLEREGVLVARPGVGTFVAAPKLTYDALHLVGFAEETMRHG
                     GAVASSVIELGIGAPPAPIQVALRLSRGETAVKIVRLRAAGDDPLLLETSYLPADRCA
                     GIEQEDLESQSLYTLLETRYGLRLMRATQTIEASAASEFEANLFGVADGAPVLVVEGV
                     AYTVDDQPVECFRAVYRADRVSLGMESHRTERATESGISQVSVVVAP"
     gene            1547..2653
                     /locus_tag="VGR22_06695"
     CDS             1547..2653
                     /locus_tag="VGR22_06695"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2128287.1"
                     /translation="MSQALRVGVIGCGSVSQKYIPHLLSLNMPTPRVEIAVLCDVLES
                     RREEVRARYGLTNFTTDYEEVIGNPDIDVVLVLTSMPLHGEITKKALAAGKHVLVEKP
                     MSMSLDEAAEIVELAKASPGFLVPAPHVVLSRTYQAMWQHLQRGDIGEPLTARGFYGW
                     SGPSWGSWYYEPGGGSMFDLGVYNVTTLTGLIGPAKRITALSGIAIPSRVVDGRPIDV
                     QTHDNAQLLLDFGDARYAVVTTGFTIQQYRVPGIEIYGSEGTIQMIGEDWAPAGYELW
                     QNSAGCWKVYNDTSAWSWTDGIRHLIESIETGTPPKITPEHAYHVLEIMVKTMESGNT
                     GRALDIESTFTPPAFPEAPILGADAHLTHDPGRE"
     gene            2650..>3485
                     /locus_tag="VGR22_06700"
     CDS             2650..>3485
                     /locus_tag="VGR22_06700"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV2128288.1"
                     /translation="MMGYASSPRPTFDAPTPILQSAITRHLWGDVDSGEVADWIYVSS
                     EHIHQLVFGLLPGGAFRHSEAYRTVFAADEVLHVLNGELVLANPETGEVHRVLPGESA
                     FFRRDTWHNAWNASTDELRVLELLAPPPSQGTTGSYARSKPYLERAQYTRDDWLGRWP
                     VGAEEAVATHTIRVLRPQDVLWRMEGEDRPLLVGILACTEHLTAGVVRLLPGQRSDVR
                     QHGGNCSLYVQSGTLNVHVSEQPAPFWYEVSRGDGFYLPQGTRYQYYNMSAEPAELIF
                     GVA"
BASE COUNT          633 a         1086 c         1173 g          593 t
ORIGIN      
        1 ccgcccgacg agatcgtcgg cacggaggac gccagccatg cccccgcggc ccggctccgc
       61 atccatgtcg cgatcagccc ggaggacagc gcgtggggcg gccaggtggc gcatgaggtc
      121 gtcacccagc atgccgggac aaacgacgtg gtgagggtgt tcttccatgc ttccgccgag
      181 gtggtcgatc cgggagagct agcgcgggtg accgaaactg ctcacgctgc tgcaggggcg
      241 tatcgggtac cgctggggcc gctgacggag gcggaggagg ccgaccagcg gggtgggtga
      301 gacggtggct ctcgtcctgg tggtcaatac cacaaggacg gggggaccac aaggacgggg
      361 gatgggacgg tgggctgcgt cggcagccgt catgacggag cacaccatgc cggcggtcgg
      421 tgacatgcat ccgacgccat ggaagccgta tgctgcctct ctccgttcga tccagccaga
      481 gcgtcccttg actgacctat acccatgcta taggctctgg tatagacctc tgtaccagca
      541 atggcggtat ggcgcccctc tctctatgtc ggcggcgtgc cagctatccg taggccgggc
      601 tacggggccg tcacctcagc agtgctcggc cgtcccggcc taccggaccc ggcggacgta
      661 ggcgaggcag cgacaccgga gaagagcgtt ggactgggga gcgaacaagc tcaaccgaca
      721 cagcccggtt ccactgtact accagctcgt cgagcacatc cgcaaatcgg tgcgcattgg
      781 ccaactcggc acgaacgcga agctaccggc ggaacgggat atcgcggagc aggcgggagt
      841 cagcagaatg acggtgcgcc aagcgctcac ctttctcgag cgcgaaggtg tcctggtggc
      901 gcggccggga gtcggcacgt ttgtggccgc tccgaagctc acctacgatg cactgcatct
      961 cgtcgggttc gccgaggaga cgatgcgcca cggtggcgcg gtggcgtcca gcgtcatcga
     1021 actgggtatc ggtgctccac cggcgccaat tcaagttgcc cttcgattgt cccgtggcga
     1081 gacggcagtc aagatcgtcc gtctccgggc cgcaggggat gatccgttgt tgctcgagac
     1141 tagttaccta cccgcagatc gctgcgcggg aatcgaacag gaggacctgg agtcgcagtc
     1201 cctctacacc ctgctcgaaa cgcgctacgg attgcggctg atgagagcga cccagacgat
     1261 cgaggcgagt gccgccagcg agttcgaggc caatctgttc ggggttgcgg atggcgcccc
     1321 cgtgctagtc gtggaaggag tcgcatacac cgtagacgac cagccagtcg agtgcttccg
     1381 ggcagtctat cgagcggacc gcgtgtcgct cgggatggag agccatcgga cggaacgcgc
     1441 gacggagagc ggcatctcgc aggtgagcgt ggtggtcgct ccctgacctc gcccgccgat
     1501 gacaacccgc gtcgaccctc gttcgacgac gataggagaa cgatcaatga gccaggcact
     1561 gcgagtcgga gtaatcgggt gtggcagcgt ctcccagaag tacattccgc acctgctcag
     1621 cctgaatatg ccgacgcccc gagtcgagat cgccgttctc tgcgacgtgc tggagagtcg
     1681 cagagaggag gttcgcgcgc gctacggcct gacgaacttc acgacggact acgaggaggt
     1741 gatcggcaat ccggacatcg atgtggtgct cgtcctgacg tcgatgccac tccacggcga
     1801 gatcacgaag aaggcgctcg cggctggcaa gcacgttctc gtcgagaaac cgatgtcgat
     1861 gtcgcttgat gaggcggctg agatcgtgga gctggcaaag gccagccctg ggttcctggt
     1921 gcccgcgcct catgtcgtgc tcagccgcac ctaccaagcg atgtggcagc accttcagcg
     1981 cggggatatc ggtgagccgc tgaccgcgcg cgggttctac ggctggtccg gcccgagctg
     2041 ggggtcttgg tactacgagc cgggtggcgg gtccatgttc gatctcggcg tctacaacgt
     2101 caccacgctg accgggctga tcggacccgc caagcgcatt acggcgctga gtggtatcgc
     2161 catcccgagc cgggtcgtcg acgggcgccc gatcgacgtg cagacgcatg ataacgccca
     2221 gctgttgctc gacttcggcg atgcgcgata tgcggtcgtg accaccggtt tcacaatcca
     2281 acagtatcgt gtgcccggca ttgagatcta cgggtccgag gggacgatcc aaatgatcgg
     2341 cgaggactgg gcgccggccg ggtatgagct gtggcagaac agcgcgggct gctggaaggt
     2401 ctacaacgac acgagtgcgt ggtcgtggac cgatggcatt cgccacctga tcgagtcgat
     2461 cgagactgga acgcctccga agatcacccc ggagcacgcg taccacgtgc tcgagatcat
     2521 ggtgaagacg atggaatcgg ggaacaccgg ccgagcgctc gacatcgaga gcaccttcac
     2581 cccgccggcg tttcctgaag cgccgatcct cggggcggac gcgcatctca cgcacgatcc
     2641 tggacgagaa tgatgggata cgcatcgtca ccccgtccga ccttcgatgc gcctacgccg
     2701 atcctgcaga gcgccatcac ccggcacctc tggggcgatg ttgactccgg cgaggtcgcc
     2761 gactggatct acgtctcaag cgagcatatc catcagctcg tcttcgggct gctgccgggc
     2821 ggcgccttcc ggcactcgga ggcctaccgg accgtcttcg cggccgatga ggtgctgcat
     2881 gtgctgaacg gtgaactcgt gctcgccaat cccgagacgg gcgaggtcca tcgtgtgctc
     2941 ccgggtgagt cggcgttctt tcgacgcgat acctggcaca acgcatggaa tgcgagtacg
     3001 gacgaactgc gcgtcctcga gcttctcgca ccgccgccat cgcagggcac cacggggtcg
     3061 tacgcgcgat ccaagcccta cctcgagcgc gcacagtaca cccgcgacga ctggttgggg
     3121 cggtggccgg tgggagcaga ggaggccgtc gccacgcaca ccattcgggt ccttcggccg
     3181 caggacgtgc tctggcggat ggagggcgag gaccgtcccc tgctcgtcgg catcctggct
     3241 tgcaccgagc acctgaccgc cggtgtggtg cgcctgctgc ccggacaacg atcggatgtg
     3301 cggcagcatg gtgggaactg cagcctttac gttcagtcag gcaccctcaa tgtacacgtg
     3361 agtgagcaac cggcaccatt ctggtacgag gtgtcgcgcg gagatggctt ctacctgcca
     3421 caagggacgc gctaccagta ctacaacatg tccgcggagc cggccgagct gatcttcggc
     3481 gttgc
//
LOCUS       DASYWL010000193        16774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_533944, whole genome shotgun sequence.
ACCESSION   DASYWL010000193 DASYWL010000000
VERSION     DASYWL010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 16774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16774
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533944"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..691
                     /locus_tag="VGR22_06705"
     CDS             26..691
                     /locus_tag="VGR22_06705"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEV2128289.1"
                     /translation="MELEAIIARALQILLALGGAYLIALWFVLVVWTFRDIESRSRNV
                     LTQIASTLLVVFFYVPGFLLYWLLRPKETLDEAYQRSLEEEYLLQDLQELPLCPTCHH
                     YVEDDYQICPHCNSQLKEACHSCGRLVDLQWSVCPYCAAPQHRHDHGTDEVHEEVVEE
                     EWIDPDEIIRRLRSGRTQELPARTAAGQQTEIIRPRTDERVKVTTASDRKRERETSGD
                     RSS"
     gene            complement(741..1631)
                     /locus_tag="VGR22_06710"
     CDS             complement(741..1631)
                     /locus_tag="VGR22_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEV2128290.1"
                     /translation="MIQKIGIIAAQSKEEANALAPEIETWLRERDIDPVVERELDANG
                     HDDVDAIVVLGGDGLMMRAANAYPDTPLLGINFGQVGFLTLVEQQHWREALEALTSGT
                     VDVEVSSTLQATLYRDEEEIDMGWAINDVVIRSGHRMVEVELYIDGDFVNPYPGDGMI
                     VSTARGSTAYCMAAGGPILTAGVKGFALVPISCHSPIRTPMVASEEALIELLIGNDHA
                     ASLILDGRPGTDLRMGDVIKVERGKHTFQLAKIGTLSFYESVRTKFNFRIRPNTTPSR
                     PHKGQRPGTVGGVSREDGSG"
     gene            complement(1643..2293)
                     /locus_tag="VGR22_06715"
     CDS             complement(1643..2293)
                     /locus_tag="VGR22_06715"
                     /inference="COORDINATES: protein motif:HMM:NF040269.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128291.1"
                     /translation="MEPRLTVVIVPFQFRQGTFCVGTLVSGDGTHVLPSQQPATREPL
                     DAAADRLMRDVTGHPPAYMEQLYTVSIEQRDPWRIIVSYLVLVAPDTDRPGEELVWVV
                     PEDVELRRETDRMLLEYALVRLRAKLGYTNIAFHLLPPTFTLTQLQQVYEQVLGQTLD
                     KRNFRRRMSASGILTRTGVRQREGSHRPAELYRFTSDADQSTYLTPLWARSDANPS"
     gene            2455..2525
                     /locus_tag="VGR22_06720"
     tRNA            2455..2525
                     /locus_tag="VGR22_06720"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2487..2489,aa:Gln,seq:ttg)
     gene            2633..4075
                     /gene="glmU"
                     /locus_tag="VGR22_06725"
     CDS             2633..4075
                     /gene="glmU"
                     /locus_tag="VGR22_06725"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEV2128292.1"
                     /translation="MSSHAQDVPDPAPESSPSDVVVAVLAAGHGTRMRSSTPKHLQAV
                     AGVPIVERVIRAGVNAGAARILVMVSDNLADLGTRIGGTGTFETVVQGPPEGTADSVR
                     QALAQAPEARWLVSLLGDSPLLSSEIVRSLIQRARSANSHLTLLTCIVPDAATYGRID
                     RDGDDRVQAIVEFKNDVPANREGPTEINSGVMVLDAAWAREELARLPRDELTGEFLLT
                     DLVDVAVRQAVPGEDWPVDAYIADRSVSLGVNDRVQLAEADALARERIRERLMREGVT
                     IIGPDTVFIDEDVRIGHDTVIYPHSILRGGTVVGERCEIGPAAVVESAQIGDAVVIRQ
                     STVRDSIIGASSDIGPYAHLRGGCHIGARVHIGSYAEMKNAELADGAKCGHVAYLGDV
                     SVGVNANIGAGTITANYDGERKNRTVIGDRAFVGSNSVLVAPVTVGEGAMTGAGSVVT
                     SDVPDGTLVMGVPARPKAPRAAGATREEQE"
     gene            4078..5013
                     /locus_tag="VGR22_06730"
     CDS             4078..5013
                     /locus_tag="VGR22_06730"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEV2128293.1"
                     /translation="MDGRLQLFTGNAHPVLAASLSRELELPLGRAIVDTWKNGETRVK
                     LNENVRGSDIFILQPTCHPVNHHIMELLLIVDAAKRASAARITAVIPYYGYAKQEKKT
                     SGREPISARLVANLLTVAGVDRVLAVDLHAPAIEGFFDIPVDHLRATPLLARAFRDEI
                     RDEVVVVSPDAGGVARAEDFRQRVRGSLAIISKQHPSPDATETLEMVGDVDGKVAVIV
                     DDMISTGGTLIEAAQMLKERGAVAIHMAATHGVFAADAIDRIAASDIERLFVTDSVPM
                     PETVDGRAKVISVAPLLAEAITRTHKGLSVSALFS"
     gene            5053..6393
                     /locus_tag="VGR22_06735"
     CDS             5053..6393
                     /locus_tag="VGR22_06735"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEV2128294.1"
                     /translation="MIVESGSWLALLGALIALLLLATAAVVETVVNRSSRSRVQAALG
                     HVPETSGRQRARDINDPRHGLSAAMDLAQVLAAIIMASLLTYVFDQAFVPDIDWLAIV
                     ATIVIYLLVGQIVPRAIGEKEFEPHVDRIIGFGRTISIVFAPIIWIIDTGVRQVKRLL
                     PGATAEQETEEISEVRSPSRSTYMDDVGDVERQMIEGILGLESMSVRDIMVPRLDIVA
                     VERSVAADDLIETITRAGHSRIPVYQESVDRILGVLYAKDLLPFVIGTTRRIPLLDLI
                     RPAFVVPESKKVSDLFAELKRARIHIAIVADEYGGTAGLVTIEDILEEIVGEIQDEYD
                     SEQPLFEQVADDVLLADGRLPIEDVEDALDIRFEEDDDFGTLGGFVHKHLGRLPIQGD
                     TFEAEGVRVEIIKVERHRIRRLRITRLRQDQEETPEQLPEVAVSTESDADDERT"
     gene            6377..7186
                     /locus_tag="VGR22_06740"
     CDS             6377..7186
                     /locus_tag="VGR22_06740"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEV2128295.1"
                     /translation="MTSAPDANPFGRLRVWYPQIGSTMDVAGALAVHGAPNGTLVETD
                     FQTAGRGRQGRQWLAPPGSALLSSWILRPPDGHDTGVLSPMIALALLRALQHLKSTIP
                     VGYKWPNDVWIGDRKAAGILLTARHAGGEPVVIAGVGVNIVRNGNAPPERAWVGDWLP
                     GVTSGRVREALAAELGGIWSRYIRCGCLADDGRAELESAMVWRDHTVEVREAGGTVRG
                     TMVRLESDGALVMQSEGGDPVIVRTGEVVRGPRIAARIVPEGAVYFPEQDR"
     gene            7225..8448
                     /locus_tag="VGR22_06745"
     CDS             7225..8448
                     /locus_tag="VGR22_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV2128296.1"
                     /translation="MDFSLTEEQIQVRDMVREFAAREIAPHIQEWDAKGEFHREILDK
                     MGQAGILGLPISEEYGGLGLDYVSLALACEELEYVDTFLRVIMSVHVGLNSLSLWQWA
                     TEEQKQRWLKPQAMGEKIATFALTEPNAGSDAGAIESTVTRDGGSYILNGAKMWISLA
                     NVADHFLVFASFDRSLKHKGLTAFVVERGMDGLTTADIKGKLGVRAGSTGEMAFNNVR
                     IPAENRIGEEGEGFKIAMSCIDQGRFTVAAGAVGLTRAALDASVRYANERETFGEKIG
                     KHQLVKQMIAKMVAGEQVSRLLVMQAAELKNRGIRNTKETSLAKWYACDVAYQSADDA
                     VQVHGSYGFSNEYPVERYLRNARGAVIYEGTREVHQLLQADYALGYREDRPLRCPQPP
                     AVGFEREVQALASDD"
     gene            8529..9716
                     /locus_tag="VGR22_06750"
     CDS             8529..9716
                     /locus_tag="VGR22_06750"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF006086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEV2128297.1"
                     /translation="MGERSVVIGGARTPFGKMGGALAPLTAVELGKVALTANIERAGV
                     DPADIEHVIFGQVLQGGAGQNPARQVAMGVGLDREVTAETINRVCGSGMRAITLADTM
                     IRVGDYRVIAAGGMESMSNAPYMVRQGRFGYRMGDGVFEDMMISDGLMCALAGCHMGV
                     HGGNVAAEERMGREEQDAWALRSHERAIAAIESGRMAKEIVPVVVAGRRGQVTTVDRD
                     EAPRADTSTEALGKLKPAFDPNSTVTAGNAPGVNDGAASIMVASEAWASERGITPRAT
                     VLGHAASAWDAPYLAYTPAMAIEMALKRAGLTTGDVDLVEINEAFASVTIISSRRLGL
                     DLEKVNVNGGAIALGHPIGASGARIVLTLVQELEERGGGIGVAAICSGGGQGDAVVIR
                     VGD"
     gene            9709..10278
                     /locus_tag="VGR22_06755"
     CDS             9709..10278
                     /locus_tag="VGR22_06755"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEV2128298.1"
                     /translation="MTDTMATEQQSPADVYRERYPGAVILPYEDAWPEIAESAFIAPT
                     AVVIGNVSIGEEASVWYHATVRGDIAPITIGDRSNVQDNTVVHVNMDAPIVIGKDVTL
                     GHSAIIHGTTIGHGSMIGMGSIVMSYSHLGSHCVVAAGDGADGGRGRRSDDGCADQAT
                     VCTRRVRPKAPGRHRRPLPGRGEHAPLVR"
     gene            10296..11087
                     /locus_tag="VGR22_06760"
     CDS             10296..11087
                     /locus_tag="VGR22_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV2128299.1"
                     /translation="MSIDIERSGAIATVTVNDPERLNALNSDSLVELERAFDEICSDD
                     AIRAVIFTGAGDRAFIVGANIKEMATKDRDEALAFGRLGHAVANRIERLPQPVIAAVN
                     GFALGGGCELALACDIRHCSENAVFAQPEVQLGIPPGWGGSQRLARVVGTGYAAEMIY
                     TGKRIEAQEALRIGLVNAVHPADALMDRVRELAQAIVEAGPQAVRASKRLMTLPWSAT
                     AVSALAEEVRTFADQFDGDEQKEGMAAFLEKRKPAFAQSSLEESA"
     gene            11084..11842
                     /locus_tag="VGR22_06765"
     CDS             11084..11842
                     /locus_tag="VGR22_06765"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEV2128300.1"
                     /translation="MTIVVLVKQVPDLNAVRVDTATGAVRTSGKAVNSYDAYAVQHAL
                     NLKDALGAEVVAVTAGPANAKDALTRALAMGADRGVHIELDDQDQRDSLDMVNIFAEA
                     LKPLEPSVVLCGQQSDDIGTGQVGPQLAELLGMPHVSSVMAVGAEGDTITVQRDTEEG
                     YQRVTAPMPVLLASGSITEDPINPSLKGMMVAKRKTVDKVTAAGRSGTTRLSWSEPKA
                     PQREAEGIIVEGETAQDAAKQLVAWMKENKLVAS"
     gene            11961..12953
                     /locus_tag="VGR22_06770"
     CDS             11961..12953
                     /locus_tag="VGR22_06770"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEV2128301.1"
                     /translation="MTQGSGVLVVAEQRDGNLTPATLELITAARSLSDSLGGNVTVGV
                     VGAGIDRVAQAAAGVAGVDEVVTVTDDRMDAYNGPAWSAAVARIVEEVGPAIVMAPAT
                     TAGRDYLPRVAARLKTGHASDAVGVEIADGKVVATRLMFEDRVQTAVAFEGGGPAVIA
                     VRPGSFARSEESGGSATVRALDFTLDDAAFTLTAEAPEAEAAGQRALAGAERIVAGGR
                     GLGEAEKFSLVEELAGQLDAAVAATRPLSDAGWRPHSDQIGQTGAQVSPKLYVAVGIS
                     GAAQHLMGVQNADYIVAINRDPEAPIFKVASFGIVGDLFEVVPAVIEELKAAQS"
     gene            13142..14440
                     /locus_tag="VGR22_06775"
     CDS             13142..14440
                     /locus_tag="VGR22_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2128302.1"
                     /translation="MAEERLDAIVVGAGPAGITAARELVAAGLSPVVLERGQYPGAKN
                     AWGGILYREPTEAMIRGFEENAPLERPIIEQRYVLLSSDAAMTASYKSMRLAEPPYNA
                     YTAFRGDFDQWYAQKAEEEGVEIYPEFAVTDLLWEDGAVVGVTTGDEEGELFANAVVL
                     ADGANSLLGQKAGLHREWQPDEQALVAKETLRLDATVIEERFNLPEGKGTALEIFGES
                     TWGMLGYGFIYTNKDSLSIGTGSLLSDLISSGRNVNDMLDRFKKHPAIQPLIGGAELE
                     EYAAHLIPEGGYKRLPTLYGDGVLLVGDAAGLVNPLNREGVNLAMMSGKLAGQAIAHA
                     TEMEDFSRVGLSRYRELLEESFVLKDLYKIRNVTEFANERPWLLTEYPELLSEAVRRY
                     LTVSGRPKKDVQDEILGLFARIPKKRLVEDALGALRAFKG"
     gene            14460..14864
                     /locus_tag="VGR22_06780"
     CDS             14460..14864
                     /locus_tag="VGR22_06780"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2128303.1"
                     /translation="MASVKRLQHVSVPMPPGGLAKARAFYGDQLGLEEKVPPQALGTE
                     DFAWFRLGDDGDELHVFTEDGMPAVGQHFCMEVDDIDAWREDLAAKGIEIAETVPIDN
                     RPRFFIRDPFMNRIEITQITGAYTPAFDDEQA"
     gene            14877..16496
                     /locus_tag="VGR22_06785"
     CDS             14877..16496
                     /locus_tag="VGR22_06785"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV2128304.1"
                     /translation="MAATDQKITYTATPEQIEAMHSHFDDAIEEARGQLGRTYPMIIA
                     GEEITDREAFEVHAPANRETLIGKFQRGTREDVDGAVAAAKAAFPAWSGRPWQERVEL
                     IRRAADIIRERKYLLSAWLIYETGKSRTEAIGEIEEGADLLSEYAQQVEDNDGFVVPM
                     GTIDPREQNVSVLKPFGVWAVVGPFNFPHALCAGMSSAALVAGNTIVFKPASATPMSG
                     YQIARAYIDAGIPNGVVNFITGGGAEVGDTLATHPDVDGVVFTGSKEVGFDLYKRFAT
                     DYPKPIITEMGGKNPTIVTKNADLNKAATGVMRSAFGFSGQKCSACSRAYVAREVYDE
                     FMDKLVAKTKELVVGDGLNRETFVGPVIDDKAVQRFKDAVDLTKKDGGTVRYGGEVLR
                     DDGYAKGTYVEPTIVDGLPLDHELFKRELFLPFIAIAPVDSLEQALEESNDTEYGLTA
                     GIFSEDEGEIDTFFESIIAGVNYANRSGGSTTGAWPGCQSFCGWKGSGSTGKGGLGKF
                     YVQQFLREQSQTRVTEPADAAEAESVEARGE"
BASE COUNT         2960 a         5134 c         5807 g         2873 t
ORIGIN      
        1 ctcctccacg caaggagccc acaacgtgga attggaagcg atcattgccc gcgcgttgca
       61 gattctcctc gccttgggtg gggcctacct cattgcgctc tggttcgtgc tggtggtctg
      121 gaccttccgg gatatcgagt cgcgcagcag gaatgtcctc acgcagatcg catcgaccct
      181 gctcgtcgtc ttcttctacg tccccggctt cctgctctat tggctgttac gcccgaagga
      241 gacgctggac gaggcatatc agcgttcgct ggaggaggag tacctgctgc aggaccttca
      301 ggagttgccg ctctgtccca cctgccacca ctacgtggaa gatgactatc agatctgccc
      361 gcactgcaac agccagctga aggaggcgtg ccacagctgc gggcggctcg tggatctaca
      421 gtggtccgtt tgtccatatt gcgcggcacc acagcaccga catgaccacg gcaccgatga
      481 ggtgcacgag gaagtcgtgg aagaggagtg gatcgatccc gacgagatca tcaggcggct
      541 ccggtcgggc cggacgcagg agttaccggc gcggacggca gccggtcagc agacggagat
      601 catccgccca cgcaccgatg agcgggtcaa ggtcacgact gccagcgatc ggaagcggga
      661 gcgcgagaca agcggtgatc ggtcctcctg atgcaggcta ggctgtgttg aaccgcgagg
      721 cgacccgtca cccgtcggac ctaccccgac ccgtcctcac gggacacgcc gccgacggtg
      781 cctgggcgct ggcccttgtg cgggcggctt ggagtggtgt tcgggcggat gcggaagttg
      841 aactttgtac gtaccgactc atagaagctg agtgtaccaa tcttggcgag ctggaaggta
      901 tgcttgccgc gctcaacctt gatgacgtcg cccatccgca ggtcggtgcc gggacggccg
      961 tcgagaatca gtgaggcggc atgatcgttg ccgatcagca gctcgatcag tgcttcctcc
     1021 gacgctacca tcggtgtgcg gatgggcgag tggcacgaga tcggcaccag cgcgaacccc
     1081 ttgacgcccg ccgtcaggat cggtccgccg gcagccatgc agtaagcggt cgagccgcgg
     1141 gctgtggaga cgatcatccc gtcgccggga taggggttga cgaaatcacc atcgatgtag
     1201 agctcgacct cgaccatgcg gtggccgctg cggatcacca cgtcgttgat cgcccagccc
     1261 atgtcgatct cttcctcatc gcggtagaga gtcgcctgca gggtcgagct cacctcgacg
     1321 tcgaccgtgc cggacgtcag tgcttccagg gcctcccgcc agtgctgctg ctcgacgagc
     1381 gtgaggaagc cgacctgccc gaagttgatg ccgagcagcg gcgtgtcggg ataggcattg
     1441 gcggcgcgca tcatcaggcc gtcaccaccg agtacgacaa tcgcatcgac gtcgtcgtgc
     1501 ccgtttgcgt cgagctcccg ctccacgacg gggtcgatat cgcgctcgcg gagccaggtt
     1561 tcgatttccg gcgcgagcgc gttcgcctcc tccttgctct gggcggcaat gatgccgatt
     1621 ttttgaatca cggtcatctc catcacgatg ggtttgcgtc ggagcgcgcc cacagtggcg
     1681 tcaggtaggt cgactggtcg gcgtccgagg tgaagcggta gagctcggcc ggtcggtggc
     1741 tgccctcccg ctggcggacc ccggtgcgcg tcaggatgcc cgaggcgctc atgcggcgcc
     1801 gaaaatttcg cttgtcgagc gtctggccga gcacctgttc gtagacctgc tgcagctggg
     1861 taagcgtgaa ggttggcggc agcaggtgga acgcgatatt ggtgtagccc agcttagctc
     1921 gcaggcgcac cagcgcgtac tcaagcagca tgcggtcggt ctcgcggcgg agctcgacgt
     1981 cctcggggac gacccagacc agctcctcgc ccgggcggtc ggtgtcgggc gccaccagta
     2041 ccaagtagct gacgatgatc cgccacgggt cacgctgctc gatgctcacg gtgtagagct
     2101 gttccatgta ggcagggggg tgaccagtga catcgcgcat gaggcggtcg gcggcggcgt
     2161 ccagcggctc gcgtgtggcg ggctgctgcg atgggagcac gtgggtgcca tccccggata
     2221 ccagggtccc gacgcagaac gtcccttgcc ggaactggaa cggcacaatg accaccgtga
     2281 ggcgcggttc cactttgagc tctgcctcca gcacgtgtgc cggtcgaccg gcgcgcagag
     2341 tgaggtcgcg agcgccgcaa cctgcaccat ctagggtaat acgaacaggt agcaggcgct
     2401 gcagcgcctc attggcagca ccgcggcaga cccgatagac tagagattgc ctgatggcgg
     2461 atggtgaaat ggtatcacaa gggcctttgg agcctttatt ccaggttcga atcctggtcc
     2521 gccagccgac ctgtgtcatg cccacgagca gccgatgata tcggcacttc accctcgctg
     2581 cgccagatcg aggttcgacg catctcgcgc actcctccgg ataatgccgc tcatgtcatc
     2641 tcacgcccag gacgtgcccg acccggcgcc cgagtcttca ccctcggacg ttgtcgtcgc
     2701 tgtgcttgcc gctggccacg gcactcgcat gcggtcttct acccccaagc acctgcaggc
     2761 agtggctggc gtgccgatcg tagagcgcgt aatccgtgcc ggggtcaatg ccggtgccgc
     2821 ccggatcctc gtgatggtga gcgacaacct tgccgacctc gggacgcgta tcggcggcac
     2881 ggggaccttc gagaccgtcg tgcagggccc gcccgagggc acggccgact ccgtccgcca
     2941 agcgctcgca caggcaccgg aggcgaggtg gctggtctcc ctgctcggtg acagcccgtt
     3001 gctcagttcc gaaatcgtgc gctccctcat ccagcgcgca aggtcggcca acagtcacct
     3061 cacgctgctg acctgcatcg ttccggacgc tgccacctac ggccgtatcg accgcgacgg
     3121 cgatgaccga gtccaggcaa tcgtcgagtt caagaacgat gtcccggcca accgggaggg
     3181 accgacggag atcaacagtg gggtgatggt gctcgacgcc gcgtgggcgc gggaggagct
     3241 ggcacgcctg ccacgcgacg agctcaccgg cgagttcctg ctgaccgacc tggtcgatgt
     3301 cgccgtacgg caggccgtgc ccggggaaga ctggccggtg gacgcataca ttgctgaccg
     3361 atcggtttcg ctcggcgtca atgatcgtgt ccagcttgcc gaggcggatg cgctcgcacg
     3421 cgagcgcatc cgagagcggc tgatgcgcga gggcgtgacg atcatcggtc cggatacggt
     3481 cttcatcgac gaggatgtgc ggattggcca tgataccgtc atctatcccc acagcatcct
     3541 ccgtggcggc acggttgtcg gcgagcgctg tgagatcggt ccggcggcgg tggtggagag
     3601 tgcacagatc ggggatgcgg tcgtgattcg ccagtcgact gttcgagact caatcatcgg
     3661 tgcctccagc gatatcggtc cgtatgccca cctgcgtggt ggctgccaca tcggtgcccg
     3721 ggtgcatatc ggcagttacg ccgagatgaa gaatgcggag ctggccgatg gcgccaagtg
     3781 cggccacgtc gcgtatctcg gggatgtgtc ggtcggcgtt aacgcgaaca tcggggccgg
     3841 aaccatcact gccaactatg atggcgagcg taagaaccgg acggtgatcg gcgaccgtgc
     3901 gttcgttggc agcaacagcg tgctggtggc gccggtgacg gtcggggagg gtgcgatgac
     3961 cggcgctggc tcggtggtga ccagcgacgt acccgatggg accctggtta tgggcgtgcc
     4021 ggcgcgcccg aaagctcccc gcgcggctgg agcgacacgg gaggaacagg agtagccatg
     4081 gacggacgcc tgcagttgtt caccggtaat gcgcatcctg tcctcgcagc gtccctgagt
     4141 cgagagctgg agctcccgct cggccgcgcc atcgttgata cctggaaaaa cggagagacg
     4201 agggtcaagc tcaatgagaa cgtgcgtggt tccgacatct tcatcctgca gccgacctgc
     4261 cacccggtga atcaccatat catggagctc ctgctcatcg tcgacgcagc caagcgcgcg
     4321 agcgcggcgc ggatcaccgc ggtgattccc tactacggct acgccaagca ggagaagaag
     4381 acctccggtc gcgagccgat ctcggcgagg ctcgttgcca atcttctgac agttgccggt
     4441 gtcgaccgtg tgctcgcggt cgacctgcac gcgccggcga tcgagggctt cttcgatatc
     4501 ccggttgacc acctgcgtgc cacaccgttg ctagcacgcg cctttcgcga cgagatccga
     4561 gatgaggtcg tggtggtcag tccggatgcc ggcggtgttg cccgggcgga ggatttccgc
     4621 cagcgggttc gggggtcgct cgcgatcatc tcgaagcagc atcccagtcc ggacgcgacc
     4681 gagacgctcg agatggtcgg cgatgtcgat ggcaaggttg cggtgatcgt ggacgacatg
     4741 atctccaccg gcggcacgct gatcgaggcc gcgcaaatgc tcaaggagcg gggcgccgtg
     4801 gcaattcata tggcggcgac acatggcgtc tttgcggcgg atgcgatcga tcggatcgcc
     4861 gcgtccgaca tcgaacgact cttcgtcacc gactcggtgc ccatgccgga gacggtggac
     4921 gggcgggcca aggtcatctc cgttgcgcca ctgctggcgg aggccatcac ccgcacgcac
     4981 aaggggctga gcgtcagtgc actcttctcg tagattgtcg agccggccag tctctccagg
     5041 catcaggaag ggatgattgt ggaatctggt agttggctcg cgcttctcgg cgcgttgatc
     5101 gcgctactgc tgctcgccac ggcggctgtc gtggagacgg tcgtcaatcg cagcagcagg
     5161 tcgagggtac aggccgcgct cggccacgtc ccggaaacca gcgggcggca acgcgcacgt
     5221 gacatcaacg acccccggca cggcctcagt gccgcaatgg accttgcgca ggtcctcgcc
     5281 gcgatcatca tggcgagcct cctaacctac gtcttcgacc aggcgttcgt ccctgacatc
     5341 gactggctgg ccatcgtcgc cacgatcgtg atctatttgc tcgtcgggca gatcgtcccc
     5401 cgcgcgatag gtgagaagga gttcgagccg cacgtcgatc gcatcatcgg cttcggcagg
     5461 acgatctcca tcgtattcgc gcccatcatc tggatcattg ataccggtgt gcggcaggtc
     5521 aaacggctcc tgccgggcgc caccgccgag caggaaacgg aagagatcag tgaggtgcgc
     5581 tctccgtctc gctcgaccta catggacgat gtcggcgatg tcgagcggca aatgatcgag
     5641 ggcatcctcg gcctcgagtc gatgagcgtg cgcgacatca tggttccgcg cctcgacatc
     5701 gtggccgtgg agcgctcggt cgccgcggac gatctgatcg agaccatcac gcgtgccggt
     5761 cactcgcgca tccctgtcta tcaggaatcg gtcgatcgca tcctcggcgt cctctacgcc
     5821 aaggatctgc tccccttcgt gatcggcacc actcgccgta tcccgctgct ggatctgatc
     5881 cgcccggcgt tcgtcgtgcc cgagtcgaag aaggtgagcg atctcttcgc cgagctgaag
     5941 cgggcccgta tccacatcgc cattgtcgcc gacgagtacg gtggcaccgc cgggctcgtg
     6001 acgatcgagg acatcctcga agagatcgtc ggcgagattc aggacgagta cgactccgag
     6061 cagccgctct tcgagcaggt ggcggatgat gtactgctgg cggacggtcg cctgccgatc
     6121 gaggatgtcg aggacgcgct cgatatccgc ttcgaggagg acgatgactt cggcacgctc
     6181 ggcggcttcg tgcacaagca ccttggtcgc ctgccgatcc agggcgatac cttcgaggcg
     6241 gagggcgtgc gcgtcgagat catcaaggtg gagcgccacc ggatccgccg cctgcgcatc
     6301 acgcggctgc ggcaggatca ggaggagaca cccgagcagt tgccggaggt ggccgtctcg
     6361 accgagagcg acgcggatga cgagcgcacc tgacgccaac ccgttcggtc gcctccgcgt
     6421 ctggtacccg cagatcggct cgaccatgga tgtagccggc gcgctggctg tccatggcgc
     6481 gccgaatggc accctcgtcg agaccgattt ccagaccgcc ggccggggaa ggcaggggcg
     6541 tcaatggctg gcgccgcccg ggagcgctct gctctcctcg tggatcctgc gcccgcccga
     6601 tggtcatgac accggcgtgc tgtcgccaat gatcgccctg gccctgctgc gagcgcttca
     6661 gcacctcaag tcaaccatcc ccgtcggcta caagtggccc aacgacgtat ggatcggcga
     6721 ccgcaaggct gccggcatcc tgctgacggc tcgccacgca ggcggggaac cggtggtgat
     6781 cgcgggagtc ggtgtcaaca tcgttaggaa tggcaacgcg ccgcctgagc gcgcgtgggt
     6841 gggcgactgg ttgcccggtg tgacatcggg cagagtgcgc gaggcactgg ccgccgagtt
     6901 gggaggcatc tggagccgat acattcgctg cggttgccta gccgacgatg gccgggcgga
     6961 gctggagagc gccatggtct ggcgtgacca caccgtggag gtccgcgaag ccggtggaac
     7021 cgttcgtggc acgatggtcc ggctggagtc ggacggtgcg ctggtgatgc agtcggaggg
     7081 cggcgatccg gtcatcgtgc ggaccgggga ggtcgtgcga gggccccgaa ttgccgcccg
     7141 aatcgtcccg gagggtgccg tatactttcc tgagcaggac aggtgacatg gatcctgatg
     7201 acgcagaaag gcgtttgagg aacgatggat ttttcactga cggaagagca gatccaggtt
     7261 cgtgacatgg tgcgggagtt tgccgcccgc gagattgcgc cgcacattca ggagtgggac
     7321 gccaagggcg aattccaccg cgagatcctc gacaagatgg gccaggcagg gattctcggc
     7381 ctgccgatct cggaggagta cggtggcctc ggcctcgact atgtcagcct cgcacttgcc
     7441 tgcgaggagc tcgaatacgt cgacacgttc ctgcgcgtga tcatgagcgt ccacgtcggc
     7501 ctcaacagcc tctcgctctg gcagtgggcg accgaggagc agaagcagcg ctggctcaag
     7561 ccacaggcga tgggtgagaa gatcgccacc ttcgcgctca ccgagccgaa cgccggttca
     7621 gacgcgggcg cgatcgagtc gaccgtaact agggacggcg gctcctacat tctcaatggc
     7681 gccaagatgt ggatctcgct cgccaatgtc gccgatcact tcctcgtgtt tgccagcttc
     7741 gatcggagcc tgaagcacaa gggactgact gccttcgtcg tcgagcgcgg catggacggt
     7801 ctcactactg ccgacatcaa gggcaagctc ggcgtccggg ccggcagcac gggcgagatg
     7861 gccttcaaca atgtgcgtat ccccgctgag aaccgcatcg gcgaggaggg tgagggcttc
     7921 aagatcgcca tgagctgcat tgaccagggg aggttcaccg tcgcggctgg cgccgtcggc
     7981 ctgacccgtg ccgcccttga tgccagtgtg cggtacgcta acgagcgcga gaccttcggc
     8041 gagaagatcg gcaagcacca gctcgtcaag cagatgattg cgaagatggt cgccggcgag
     8101 caggtctcgc gccttttggt gatgcaggcc gcggagctca agaaccgggg aatccggaac
     8161 acgaaggaga cctcgctggc gaagtggtat gcctgtgatg ttgcatacca gtcggcggac
     8221 gacgccgtgc aggttcatgg ctcctacggg ttctcgaacg agtatcccgt ggagcgttac
     8281 ctgcgcaacg cgcgcggtgc ggtgatctac gagggcactc gcgaggtgca ccagctgctg
     8341 caggcggact acgcgctcgg ctaccgggag gacaggcccc tccgctgccc gcagccaccg
     8401 gcggtgggct tcgaacgcga ggttcaggcg ctggcgagcg acgactgacg ggacggacag
     8461 ccggttgatg gtcggtaccg cggttgcctg aaatcattcg agaggctgga cggaaggaac
     8521 acattggcat gggcgagcga agcgtggtga tcggtggcgc gcggacgcca ttcggaaaga
     8581 tgggtggcgc actggcgccc ctgaccgctg tcgagcttgg caaggtggcg ctgaccgcca
     8641 acatcgaacg ggcaggtgtc gacccggccg atatcgagca cgtcatcttc gggcaggtgc
     8701 tgcagggtgg cgccggtcag aaccccgctc ggcaggtcgc catgggtgtc ggtctcgacc
     8761 gcgaggtgac ggccgagacc atcaaccggg tctgcggctc cggcatgcga gcgatcaccc
     8821 tggcggacac catgatccgc gtcggggact accgagtgat cgccgccggc ggcatggaga
     8881 gcatgagcaa tgcgccgtac atggtgcggc aagggcgctt cggctatcgg atgggtgacg
     8941 gcgtcttcga ggacatgatg atctccgatg ggctcatgtg tgctctcgcg ggttgccaca
     9001 tgggtgtcca cggcggcaac gtcgcggctg aggagcgcat ggggcgcgag gaacaggatg
     9061 cctgggcgct tcgttcccat gagcgggcga tcgccgcgat agagtcgggc cgaatggcga
     9121 aggagatcgt gccggttgtg gtcgcgggtc gcagggggca ggtgacgacg gttgatcgcg
     9181 acgaggcgcc gcgtgctgac acctctacgg aggcgctcgg caagctcaag cccgccttcg
     9241 atcccaactc cacggtcact gccggcaacg cgccgggcgt caacgatggt gcggcctcga
     9301 tcatggttgc atcagaggca tgggcgagcg agcgcggtat cactccacgg gcaacggtgc
     9361 tcggtcacgc cgcttcggca tgggatgcgc cctaccttgc gtacacgccg gcgatggcga
     9421 tcgagatggc actcaagcgt gccggtttga ccaccggcga cgtcgatctc gtcgagatca
     9481 acgaggcctt cgccagcgtc acgatcatct ccagcaggcg cctcggactc gatttggaga
     9541 aggtgaacgt caacggtggc gcgatcgcgc tggggcatcc gattggtgcc agtggcgcac
     9601 gcatcgtgct gacgctcgtg caggagctcg aggagcgcgg cggcggcatc ggtgtggccg
     9661 ccatctgctc gggtggcggg cagggtgatg cggttgtcat cagggttggt gactgacacg
     9721 atggccaccg agcagcaatc ccccgctgat gtctatcgcg agcgctatcc gggtgcggtg
     9781 atccttcctt acgaggacgc ttggccggag atcgccgaga gcgccttcat cgcgccgacc
     9841 gccgtcgtca tcggcaacgt ctcgatcggc gaggaggcga gtgtctggta tcacgccacg
     9901 gttcgcggtg atatcgcgcc gatcaccatc ggcgaccgca gcaacgtgca ggacaacacc
     9961 gtcgtccacg tgaacatgga cgccccgatt gtgatcggca aggatgtgac cctcggtcat
    10021 agcgcgatca tccacggcac caccattggg catggctcga tgatcggcat ggggtcgatc
    10081 gtgatgtcct actcacacct ggggagccac tgtgtagtgg ccgctggtga cggagcggac
    10141 ggtggtcgag gacggcgcag tgatgatggg tgtgccgacc aggccacggt ctgcactcga
    10201 cgagtccgcc caaaagcacc tggacggcat cgcaggccgc tacctggccg tggcgagcac
    10261 gcaccgctcg tccgttgaag gagccaaccg ctgacatgag tatcgatatc gagcgttctg
    10321 gggcaatcgc cacggtgacg gtgaacgacc cggagcgcct gaacgccctt aactccgact
    10381 cgctcgttga gcttgagcgc gcgtttgacg agatttgcag cgacgatgcg atccgcgcgg
    10441 tgatctttac cggcgcgggt gatcgcgcct tcatcgtagg ggcgaacatc aaggagatgg
    10501 cgaccaagga tcgggatgag gcccttgctt tcgggaggct ggggcacgcc gtcgcgaacc
    10561 ggatcgagcg gctgccccaa ccggtgatcg cggcggtcaa tggcttcgcg ctcggcggcg
    10621 gctgcgagct ggcactggcc tgcgacatcc gccactgctc ggaaaacgct gtcttcgcgc
    10681 aaccggaggt ccagctcggc atcccaccgg ggtggggcgg gtcccagcgg ctggcccgcg
    10741 tcgtcgggac gggctatgcg gccgagatga tctacaccgg caagcggatc gaggcgcagg
    10801 aggcgctccg catcgggctg gtcaacgccg ttcacccggc tgacgcgctg atggacaggg
    10861 tgcgggagct cgcgcaagcg atcgtcgagg ccgggccgca ggcggttcgt gcctccaagc
    10921 gactcatgac cctgccgtgg agtgcaaccg cggtcagcgc gctcgctgag gaggtccgca
    10981 cctttgccga ccagttcgac ggcgacgagc agaaggaagg catggcggcg ttcttggaga
    11041 agcgcaagcc ggccttcgcc caatccagtc tggaggagag tgcatgacca tcgtggtgct
    11101 tgtgaagcag gttccggact tgaacgccgt tagggtcgat acggcgaccg gggccgtgcg
    11161 cacgtcaggc aaggcggtga attcctacga tgcctacgcc gtgcagcatg cgctgaacct
    11221 gaaggatgcc ctcggcgccg aggtggtggc agtcaccgcc ggaccggcga atgccaagga
    11281 cgcgctcacc cgtgccctag cgatgggtgc tgaccgaggc gtgcacattg agctcgacga
    11341 ccaggaccag cgcgattcgc tcgatatggt gaacatcttc gccgaggcgc tgaagcccct
    11401 cgagccatcg gttgtgcttt gcggtcagca gtcggacgat atcgggaccg ggcaggtggg
    11461 cccgcagctg gcggagctcc tcggcatgcc gcacgtttcc agcgtgatgg cggtcggcgc
    11521 tgagggtgac acgatcacgg tgcagcgcga caccgaggag ggctaccagc gggtaaccgc
    11581 gccgatgccc gtgctgctgg catccggttc gatcaccgag gacccaatca atccctcgct
    11641 caagggcatg atggtcgcca agcgcaagac ggtcgacaag gtgactgctg ccggcaggag
    11701 cggtacgact cgcctgagct ggagcgagcc gaaggcaccg cagcgcgagg ccgagggcat
    11761 catcgtcgag ggagagaccg cgcaggacgc cgcgaagcag cttgtcgcct ggatgaagga
    11821 gaacaagctg gtcgcctcgt aggggcggcc gcaggcccaa gtcgaatggg tctactcgtg
    11881 tacagcctag tgccgaccgg tgcagacggt ttaggacacg atcgatcacc ggggaccgga
    11941 tagcacgaag gatactgttg atgactcagg gatcaggagt tctcgtcgtc gccgagcagc
    12001 gcgatggcaa tctgacgccg gccacgctgg agttgatcac ggcggctcgg tcgctgtccg
    12061 acagcctcgg cggcaacgtg acggtgggtg tcgtcggagc cggcattgat cgtgtcgcac
    12121 aggcggcagc cggtgttgcc ggtgttgacg aggtcgtcac ggtcacggac gaccggatgg
    12181 acgcctacaa cggtccggca tggtccgcgg cagttgccag gatcgtcgag gaggttggtc
    12241 ctgcgatcgt gatggcgccc gcgacgactg cgggacgtga ctatctgccg cgtgttgccg
    12301 cgcgcctgaa gaccggccac gcctcggacg ccgtcggtgt tgagatcgcc gacggcaagg
    12361 tggtggcgac gagactgatg ttcgaggatc gtgtgcagac ggcggttgcg ttcgagggcg
    12421 gcggacccgc ggtgatcgcg gttcgccccg ggtccttcgc ccgctcagag gagagcggtg
    12481 gctcggcgac cgttcgcgcg ctcgatttca cactggatga cgcggccttt acgttgacag
    12541 ccgaagcgcc ggaagcggag gcggccggcc agcgggccct cgctggagcc gagcgcatcg
    12601 tcgccggcgg gcgaggactc ggcgaggcag agaaattctc gctcgttgag gagctcgctg
    12661 gtcagctcga cgcggcggtg gcggcgactc gcccgctttc ggacgctggc tggcgcccac
    12721 actcggatca gatcgggcag accggcgctc aggtctcacc caagctgtac gttgcagtag
    12781 ggatcagcgg cgcggcccag cacctcatgg gggtgcagaa cgccgattac atcgtggcga
    12841 tcaaccggga cccggaggcg ccgatcttca aggtcgcctc cttcggcatc gtcggtgatc
    12901 tcttcgaggt cgttccagcc gttattgagg agctcaaggc cgcgcaatcg tagggctcgg
    12961 ggacagcgtg cagcgtcggt cctgtggcga cccggccggc tgctacgcga cctcccggcg
    13021 tcgaatctcc gtagcgccga cctcggggcg gcccgtgagc cgcagggggc gggccacgtc
    13081 tccacccatt cagcgcgtag cggccatatt tgcaggcaga caggtgagga gcacccagca
    13141 catggcggaa gagcggctag acgcgatcgt ggtcggcgcc gggccggccg gcatcaccgc
    13201 cgcccgagag ctcgtggcgg ccgggctctc cccggtcgtg ctggagcgcg gccagtatcc
    13261 cggcgccaag aatgcctggg gcggcattct ctaccgtgag ccgaccgagg cgatgatccg
    13321 cggcttcgag gagaacgcgc cgctggagcg accgatcatc gagcagcgct acgtgctgct
    13381 ttcctcggac gccgcgatga cggcgtcata caagtcgatg cggctcgcgg agccgccgta
    13441 caacgcctac accgcgttcc gcggtgactt cgatcagtgg tacgcccaga aggccgagga
    13501 ggagggcgtc gagatctacc cggagttcgc ggtcaccgac ctgctctggg aggacggcgc
    13561 ggtggtcggc gtgacgacag gggacgagga gggtgagctc ttcgccaacg cggtcgtgct
    13621 ggcagatgga gcgaactcgc tgctggggca gaaggccggg ctccaccgcg agtggcagcc
    13681 ggatgagcag gccctggtcg ccaaggagac gctgcgtctc gatgccaccg tgattgagga
    13741 acgcttcaac ctgccggagg gcaagggcac cgcgctggag atcttcggcg agagcacctg
    13801 gggcatgctc ggctacggct tcatctacac caacaaggac tcgctctcga tcggcaccgg
    13861 ctcgctgctc tccgacctca tcagcagcgg ccgcaacgtc aacgacatgc tcgaccggtt
    13921 caagaagcac ccggcgatcc agccgctgat cgggggtgct gagctggagg agtacgctgc
    13981 gcacctgatc ccggagggcg gctacaagcg gttgccgacg ctctacggcg acggggtgct
    14041 gctggtcggc gatgccgccg ggctggtcaa cccgctcaat cgcgagggcg tgaatctggc
    14101 gatgatgtcc ggcaagctcg ccgggcaggc gatcgcgcac gcgaccgaga tggaggattt
    14161 ctcccgggtc gggctctcgc ggtaccgcga gctgctggag gagagcttcg tgttgaagga
    14221 tctctacaaa atccggaacg tgacggagtt cgccaatgag cggccgtggc tcctcaccga
    14281 gtatccggag ctgctgtcgg aggcggtgcg tcggtacctg acggtgagcg gcaggccgaa
    14341 gaaggatgtt caggacgaga tcctcgggct tttcgcgagg attccgaaga agcggctggt
    14401 cgaggatgcc cttggcgcgc tgcgcgcatt caaggggtag tgaaaggacg accgatatca
    14461 tggcgtcagt caaaaggctt caacatgtca gcgttccgat gccgccgggc ggtctcgcca
    14521 aggcacgcgc cttctacggg gatcagctcg gtctcgagga gaaggtcccg ccgcaggcgt
    14581 tgggcaccga agacttcgcg tggtttcgcc taggcgacga cggcgatgag ctccacgtct
    14641 tcacggagga tggcatgccg gcggtcgggc aacacttctg catggaggtg gacgacatcg
    14701 atgcctggcg ggaggacctc gccgcgaagg gcatcgagat tgcggaaacg gtgccgatcg
    14761 acaaccgacc tcgcttcttc atccgggatc cgttcatgaa ccggatcgag atcacccaga
    14821 tcacgggtgc ctatactcca gccttcgatg acgagcaggc ctgaaggagt tacgagatgg
    14881 cggcaaccga ccagaaaatt acctacaccg ctacgcccga gcagatcgag gcgatgcact
    14941 cgcacttcga cgatgccatc gaggaggctc gcggccagct cggccggacc tatccgatga
    15001 tcatcgccgg tgaggagatc accgaccgcg aagccttcga ggtccatgca ccggccaacc
    15061 gcgaaacgct gatcggcaag ttccagaggg gcacgcgcga ggatgtcgat ggggcggtcg
    15121 cggcggcgaa ggcggcgttc cccgcgtggt ccggccgacc atggcaggag cgggtcgaac
    15181 tgatccgcag ggcggccgat atcatccggg agcggaagta tctcctatcg gcgtggctga
    15241 tctacgagac gggcaagtcg cgcaccgagg cgatcggcga gatcgaggag ggggcggacc
    15301 tgctcagcga gtatgcccag caggtggagg acaacgacgg ctttgtcgtg ccgatgggca
    15361 ccatcgaccc gcgtgagcag aacgtctcgg tcctcaagcc cttcggcgtg tgggcagtgg
    15421 tcggtccctt caacttcccg catgcgctct gtgccggcat gtcatccgcc gcactggtcg
    15481 cgggcaacac gattgtcttc aagccggcga gcgcgacacc gatgtcgggc tatcagatcg
    15541 cccgcgcgta catcgatgcc ggcattccga atggcgtggt gaacttcatc accggtggcg
    15601 gcgccgaagt gggtgacacg ctcgcgacgc acccggacgt cgacggcgtg gtgttcaccg
    15661 gctccaagga ggtcggcttc gatctctaca agcggttcgc gaccgactac ccaaagccga
    15721 tcatcaccga gatgggtggc aagaacccga cgatcgtcac gaagaacgcg gatctcaaca
    15781 aggcggcgac gggcgtgatg cgctcagcgt tcggcttctc cggccagaag tgctcggcct
    15841 gctcccgcgc ctacgtcgca cgcgaggtct acgacgagtt catggacaag ctcgtcgcga
    15901 agacgaagga gctcgtggtc ggcgacggcc tgaaccggga gacgttcgtc gggccggtga
    15961 tcgacgacaa ggccgtgcag cgcttcaagg atgccgtcga cctgacgaag aaggacggtg
    16021 gcaccgttcg ctatggcggt gaggtgctaa gggacgacgg ctacgccaag ggcacctacg
    16081 tggagccgac gatcgtcgac gggctgccgc tcgaccatga gctgttcaag cgggaactct
    16141 tcctgccgtt catcgcgatt gcaccagtcg actcgctgga gcaggcactc gaggagtcta
    16201 acgacacgga gtacggcctc accgcgggca tcttcagcga ggacgagggc gagatcgaca
    16261 cgttcttcga atcgatcatc gcgggtgtga actacgcgaa ccgcagcggc ggctcgacca
    16321 ccggcgcgtg gcccggctgc cagtccttct gcggctggaa ggggagtggc tcgaccggaa
    16381 agggtgggct tggcaagttc tacgtgcagc agttcctgcg ggaacagagc cagacacggg
    16441 tcacggagcc tgctgacgct gcggaagctg agtcggtcga agcgcgcggc gagtagacgt
    16501 gcgtcgatca acggcggtgc tggcaggtag cggtgtgacc accatcgctg ccgtggtttc
    16561 tgcatccccg gcggcgatgg tttcccggtt ggaacaccga attcaagttg ccgttgttcg
    16621 cgtgcaggaa cggaggccgt gatccgcgat tactcggggt cctgcagatt cgcgaagacg
    16681 ttgttgaggg actcggcgag cgtcgggtgg gcgaaaacac cgtcgcgcag ggcggtgtag
    16741 ggaagattgc ccatcatggc gatctggagc atcg
//
LOCUS       DASYWL010000194         1679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_590977, whole genome shotgun sequence.
ACCESSION   DASYWL010000194 DASYWL010000000
VERSION     DASYWL010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590977"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1653)
                     /locus_tag="VGR22_06790"
     CDS             complement(<1..1653)
                     /locus_tag="VGR22_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128305.1"
                     /translation="MSDPLAGFHGETKVINSDPDPVIATKYALPEPPILVKVFVPRRS
                     ATGGRYVLAVERSSCSGIEDTCALRPAYEIPSGAPTDKPLNEADTAWFSLHPDVLTSG
                     GTQRMGFDVITNDPGTFRMELLDKTGTVLDDALRLDGAAPLHMDVDRHRGGEPLLLTV
                     KRWGPVNGKFPSFAVRWTTNLKRLYGTMSYNLALGANDEVGPDWSGDDEITITGLWDF
                     RHEVKIDRYQDVDTNDQLPVPEFGTVTYADAFSVKLTESSPGEAAGLSAQKTIPDLGP
                     LEPHTQNLGELITLTAPFGDGLYTFKYRITHEDPYKQSTAMSQASVARVRAAQSPASA
                     TPSPIAPVAQNLGATQIAFSTMKRPSGSGALASGSFDLGRMSGSPVAEELAMVATPLD
                     DAGPALSPNGNDVVFAARGRGASRLLISSVTGAGRTRALTRGDAVDGSPAWSPDGRAI
                     VFRREDARGYGDLFVVKSTGGRARRITRTAADESSPSRAPNGHSIVFARLGQRRRTST
                     LHIFDFRTRSTRPLLAGRSSKSQPDWSPDGKTIAFTIGDAGGE"
BASE COUNT          304 a          537 c          521 g          317 t
ORIGIN      
        1 ctcgccgcca gcatcgccaa tcgtgaacgc gatcgtcttt ccgtctggag accagtcggg
       61 ctggctcttc gagcttcgcc ctgcgagcag aggccgcgta ctacgggtgc gaaaatcgaa
      121 tatgtggagc gtcgacgtcc gccgtcgttg gcccaagcgc gcaaacacaa tgctgtggcc
      181 gttgggcgcc cgagatgggc tgctctcgtc ggctgccgtc ctagtgatcc tgcgggcgcg
      241 accaccggtt gacttgacca cgaacaagtc gccgtatcca cgcgcatcct ctcggcgaaa
      301 aacgatcgcg cgaccatcag gcgaccatgc cgggctgcca tcgactgcgt cgcctcgggt
      361 aagggcgcga gtccgcccag ccccggtcac agagctgatc agcagccgcg acgctccgcg
      421 ccctcgggct gcgaagacga cgtcgttgcc gttcggcgac aacgcgggcc cggcatcgtc
      481 caagggcgtc gcgaccatcg cgagctcctc cgcaaccggt gatcccgaca tccgccctag
      541 atcaaatgaa cccgaggcaa gggctcctga cccgctcggc ctcttcatcg tcgagaacgc
      601 aatctgtgtc gcgcctagat tctgcgccac gggcgcgatc gggctgggcg tcgcagacgc
      661 tggggactgc gccgcgcgca cgcgtgcgac cgacgcctgc gacatagcgg tcgactgctt
      721 gtagggatct tcgtgcgtaa tgcggtactt aaaggtgtat aaaccatcgc caaagggcgc
      781 tgtcagcgta atcagctcac caaggttttg cgtgtgtggc tcgagcggac cgagatctgg
      841 gatcgtcttt tgtgccgaca ggccggccgc ctctccggga ctcgattctg tcagcttgac
      901 gctgaacgcg tcggcgtacg tgaccgtacc aaactccgga acagggagct ggtcgttcgt
      961 gtcgacgtct tgataacggt caatcttcac ctcgtgccgg aagtcccaca ggccggtgat
     1021 cgtgatctcg tcatcccccg accagtcagg gcccacctcg tcgtttgccc cgagcgcgag
     1081 attgtacgac atcgtgccgt acaagcgctt gaggttggtc gtccagcgga ccgcgaatga
     1141 cggaaacttg ccattcaccg gtccccaccg cttcacggtc agtaacaaag gctcgccgcc
     1201 tcggtgccga tcgacatcca tgtgcagtgg cgcggcaccg tccaatcgga gcgcgtcgtc
     1261 aagtactgtc ccggtcttat caagcagctc catccgaaag gtgccggggt cgttcgtgat
     1321 gacatcgaac cccatccgct gtgtcccccc actggtgagc acatcagggt gcagcgaaaa
     1381 ccacgccgtg tcggcttcgt tgagcggctt gtcggtgggt gcgccggaag gaatctcgta
     1441 agcgggcctc agggcacagg tgtcttctat cccagaacat gatgagcgct ccacggcgag
     1501 tacataacgg ccgccggtag cggagcggcg cggcacgaag accttgacca ggatcggtgg
     1561 ctcgggtagt gcgtacttcg tcgcgatgac ggggtcggga tcagagttga tgaccttggt
     1621 ctcgccgtga aagccggcaa gcggatcgct catgttgctc gcttcataaa gctctacgc
//
LOCUS       DASYWL010000195        14783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_534109, whole genome shotgun sequence.
ACCESSION   DASYWL010000195 DASYWL010000000
VERSION     DASYWL010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 14783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14783
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534109"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            269..1159
                     /locus_tag="VGR22_06795"
     CDS             269..1159
                     /locus_tag="VGR22_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2128306.1"
                     /translation="MYEQLTDRVGVMTGGVNIGVIRTESGTAILVDTGANEGNARKML
                     RHVREELSADVEAILSTHGHADHFGGHAFVVKRTEAAVYASRFEAAVLEHPELQPIFL
                     FGGAAPPEVLRQRFVFAAPVQVDAHVDAGPLEVGGVPIEVVSLPGHSPGQVGYLVDGV
                     FFCADVVFPQSAIDKYRIPYLFDLTGHLASLDLALQVAATAVVPGHGVVERSIGELVE
                     LNRAVADETLDVIRRSLTEPLALEPLARRLFERMDVPMTRHVSYYLLRPTIGAYLTHL
                     ESLGEIEHVMDGRMAKWRLA"
     gene            complement(1166..2518)
                     /locus_tag="VGR22_06800"
     CDS             complement(1166..2518)
                     /locus_tag="VGR22_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV2128307.1"
                     /translation="MSASDAISGNGKRIGAIGGGFAGLTAAYRLARAGHQVTLWERNQ
                     YGGQAGTFPVGDTELEIFYHHLFRSDRAIQDLAEELGIGDKLVWHPSNVGYFTDGMIY
                     PLNGALDVLRLGSIPFIDRIRLGAVTFYLQRKKDWKPYEKVTAHEWLQRALGKRAYDK
                     TLGAQLRAKFGTAYDRVAMVWFWGKIWLRTTSRSNPLEQEMLGYFEGSFRTMVDALVR
                     GCEEAGVDMRLGAAITDLGQSTATNTWIVATADRREQETFDALIATVPSPVFQKMVPG
                     LPAEYVAKLNTLHYEAAVVAILELDRPLTDIYWLNIADDDMPFTGIIEHTNFIDPKAY
                     GGRRFVYLSKYLEPDHPYFRMPDEDLIREYVPYLTRVNPDFSEEWIQNTWVFRERSAQ
                     PIVPLNYSEKIPPHRTPLRGLYLANTTQIYPEDRGTNYSVRLGNDIAKLVDSDLRSNG
                     "
     gene            complement(2526..3479)
                     /gene="secF"
                     /locus_tag="VGR22_06805"
     CDS             complement(2526..3479)
                     /gene="secF"
                     /locus_tag="VGR22_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEV2128308.1"
                     /translation="MRDLARRRNLWYLISAILLLPGIISLIFNGLELGIDFTGGSVWE
                     IRYTEEVNTSEIREVLVAEGRTEAQVQQVGDPDENRYQIRLRELAESSPEKERLTEAL
                     ETLAPLDPEAEALETIGASVGTSIRNRSLLAVGAASVGVLLYIAWAFRNTGNPLLYGT
                     AAIVAMLHDALFVLGVFSILGVVADVEIDALFVTALLTVIGFSVHDTIVVFDRMRENR
                     LMDDDASMEEIVNRSLVQTLVRSLNTSITVIFTLAALLVFGGESTRNFVLALLIGMIA
                     GTYSSIFNAAQLLVSWNNGEIQQLFRRVARRPDPGAEPVEA"
     gene            complement(3476..4876)
                     /gene="secD"
                     /locus_tag="VGR22_06810"
     CDS             complement(3476..4876)
                     /gene="secD"
                     /locus_tag="VGR22_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEV2128309.1"
                     /translation="MQLRLWHRLVLVVLITLVAGWIALPGDSWDFADYRDNHPVRLGL
                     DLQGGLQVTLEARPPEGVDVNERTLEATRNTLERRVNALGVSEPLVQTRGSDQIVVEL
                     PGIDDPDTAIEVLQDTALLEIVDSRGQYLPPGTLVNTSLGSADEVPLATPVAGQEPPP
                     ADDTVYETIISGSDLTDAYVTTTQTGQVAVAFELTSDAGRDFHQYTSTHLGQPMSIVV
                     DKTVISSPVINAPISTSGVIEGVGAQEAETLALQLRSGALSVPMDVVSSRTIGPSLGQ
                     TSIEKSLVAGALGLATVGLFMILFYRLPGVVSVVALLIYTALVFALFRIIPVTLTLAG
                     IAGFILSIGMAVDANVLIFARLKEELLVGRRLDRAVQAGFEHAWPSIRDSNVSTMITC
                     VILYWFGQFTGATIITGFALTLFVGVAVSMFTAMFVSRTLLQAVLESGWFTSNWWLGI
                     DPDQQRIAAAEAGGRA"
     gene            complement(4968..5591)
                     /gene="nadD"
                     /locus_tag="VGR22_06815"
     CDS             complement(4968..5591)
                     /gene="nadD"
                     /locus_tag="VGR22_06815"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEV2128310.1"
                     /translation="MTAARRIGILGGTFDPVHIAHLHIAVCAKHELGLDEVRFVPAGS
                     PPHKPGQPVTSGSHRLRMLEIATVGFDVFVPDPIDLADEVPSYTSDLLARIRKAHPDD
                     QLWFIMGGDSLTDFPRWHQPRRILSLARLAVVERPGWDAAEALGSSPLPELAGRVDRF
                     PSVPIDLSATLIRARVAAGQPVDWLVPAEVLAYIHIHGLYALGHGTD"
     gene            complement(5588..6364)
                     /locus_tag="VGR22_06820"
     CDS             complement(5588..6364)
                     /locus_tag="VGR22_06820"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEV2128311.1"
                     /translation="MPEISIDYDALREDLWDLARIGFWIAVWLIGGWWLGSLARAWVR
                     STLSRRSMGWNGTVLLSRLASIAIRIIAVLLVLNTLGVSGTGLLAVVSAFTVAIGLSL
                     QDVLKNFFAGIYLLLERPFRSGDRIVVKDVSGEVQGIDIRTTLVKNRDNELVLIPNAT
                     IFTEILRNDSFYGVRRMEFTIKSETRTVHEIEERMHGALGSVDGVKRPLPAPRIVSKA
                     GETLTMTTSLVVENTDEAENAASQALIDALPGDTIEVNLT"
     gene            7239..7958
                     /locus_tag="VGR22_06825"
     CDS             7239..7958
                     /locus_tag="VGR22_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128312.1"
                     /translation="MAAASIAGAAAVTAIMGVFTALFVIPLVVPSLILGAMDRSRIGQ
                     GWPAAVGVNLATMLLLLPTLVIRQSAVHVPYLTSEHGTMFVAVASTIAVIFTMVSIAL
                     VAAWASRQDPESSPMLFMPAAMLVPLMTSASEFAQLDVALAAAGTIFALTALLTLISS
                     LMPPIYTMFVGPLAVAVEVLFVSLIRQDRIFPTGATPIGMALFGFVILAAIALTVLLP
                     AMSSWMQRVEMFRGRWLRRVA"
     gene            complement(8173..10683)
                     /gene="gyrA"
                     /locus_tag="VGR22_06830"
     CDS             complement(8173..10683)
                     /gene="gyrA"
                     /locus_tag="VGR22_06830"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEV2128313.1"
                     /translation="MDIGNVRTASIESEMRQSYLDYAMSVIVQRALPDARDGLKPVHR
                     RVLYAMHQMGLRSNTRHRKSAGIVGEVIKNFHPHSDTAAYDTLVRMAQGFSLRYPLVD
                     GQGNFGSVDGDSAAAMRYTEARMTAIAEEMLGDINKRTVDEYPNYDATMMQPSVLPSR
                     IPNLLINGSAGIAVGMATNIPPHNLHEVADAVSHLIDNPDATVEELIEIVPGPDFPTG
                     GTILGREGIHAAYATGRGRVVIRAKAFIEDMERGNRSQIVVTELPYQVNKAALLERIA
                     EMVRDGKLDGISNLRDESDRSGMRMVIELKRDAQPKKVLNNLFKHTALQQTFGVNMLA
                     LVDRGMQPRVMTLKRALQEFVGHRQEVITRRTEFELERARHRAHILEGLKIALDGIEE
                     VINTIRRSRTTDTARSNLMKNFKLSRVQADAILDMRLARLAALERQKIENEYKEVMAE
                     ISRLEELLANPGLILGLIKDDMEDLKEKYGDERRTRIQNVSGELSDDDLIAEVDVLVT
                     ITNRGYVKRINGDSYRTQRRGGRGITGLTMRDQDGIQHILSANTHDSLLVFTNRGRVY
                     QIKVHELPDAGRTAKGLPIVNVINLQPDEKVATLLKVRHHDPGNYLFFATRKGRVKRT
                     TLDQFSSIRSDGKAAIGLDDSDELVWVRITDGNSDVIMVTQKGQSIRFHESDARPMGR
                     TAAGVIGIRLDGNDSVIAFDVIDPQTDEDLLVVSAEGMGKRTSIDQYRVQTRGGKGIQ
                     AMRLTERTGDIVAAGMVAEDDAVMLMSTGGIAIRFAAGQISKIGRTTQGVRLMRLGGE
                     QVASMTIMEPRDPTSEAQIGDLEESAPGADGAEPDATV"
     gene            complement(10816..11862)
                     /locus_tag="VGR22_06835"
     CDS             complement(10816..11862)
                     /locus_tag="VGR22_06835"
                     /inference="COORDINATES: protein motif:HMM:NF024813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV2128314.1"
                     /translation="MAIIDLIEHPNEASNELVYRVPEGGSGEFRLGSQLVVRESQRAV
                     FFRDGKALDVFRPGRHTISTNNVPLLAGLIGLPFGGDSPFRAEVYFVSVREFTDMKWG
                     SQQPLVYRDSELGMVRLRAYGTYSMKVADPQLFVNEVVGTRGSYRTAAIEDYLRSVIL
                     NEFNDMLGDVHTSILDIQRMTREIADASRAALADDFTRLGLDLVSFQISAITPPEEVQ
                     QRIDERSSMGAIGDMGAYTQYKTAQAIGDAARNEGGSGDATSTGVGLGAGLGIGQAMA
                     GAIGSSYQQSQQRQQQGQQLETRQCPNCNSTIPASAKFCPECGVNLAAKVCPECGTSN
                     SPGAQFCTNCGTKL"
     gene            complement(11935..12636)
                     /locus_tag="VGR22_06840"
     CDS             complement(11935..12636)
                     /locus_tag="VGR22_06840"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEV2128315.1"
                     /translation="MAILDRISRLVRANVNDLIDQAEDPEKMIDQILRDMQSSITTAR
                     AQVAAMIAQEKELEAEVNESEELVREWEAKARRAVEAGKDDLAREALRRKHDAQENLQ
                     LYHEQHTTQEHTVEKLKSQLQKLESKYQVTLSQRDSLIARQRRARAQQQVAERVATSF
                     SPLDASSELERMERKIRSNEARAAATAELEDTSLDAQFRELDYDVEIERELEALKSGS
                     SQSAGALGEGQGTNS"
     gene            complement(12661..13632)
                     /locus_tag="VGR22_06845"
     CDS             complement(12661..13632)
                     /locus_tag="VGR22_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128316.1"
                     /translation="MAERIASRVENLLNRVPGYTGYRQKESMRDDDRRLREQIARELQ
                     QSISDLTRIGSKLAASRQLDRISAAEDTVSRLRHLESRVRTASYGYGGIFSDRDVNEH
                     ALAQIRQFDVAFQERVAGLTESVSAVASADSFDPSALEGIRREIDTLNTLFDMRTDVI
                     ETASPSDDPDVLALLEEPRALSAHERQLLGIRSGGTGAILGDNYQFTSHIALTSPAGE
                     PVATLVQLDRGPEWLAITDDGREVRAWRVTEQEVVTPGASGQPASASISGPQGSTSGV
                     PATYRLSVVGAGQDARAEVKLRAAGSDRAYSGTNVPLLDLQIFSQGG"
     gene            complement(13657..14178)
                     /locus_tag="VGR22_06850"
     CDS             complement(13657..14178)
                     /locus_tag="VGR22_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128317.1"
                     /translation="MAASGRVLGIVLIGAGIVLGIAITAWIMFGVSEGNLRGSGAALG
                     LSLLLGIIVLPLLGAGIFLFIRGNAEARELREINKQRKLLDMVKTRGQLSIDDVVLEM
                     NSTRDNVQDHLHALVGRGLFSGYVDWNRGMLYSVDARELSQRSTCPNCGGQLELAGKG
                     LIKCPYCGAEIFL"
     gene            complement(14272..>14783)
                     /gene="fabZ"
                     /locus_tag="VGR22_06855"
     CDS             complement(14272..>14783)
                     /gene="fabZ"
                     /locus_tag="VGR22_06855"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEV2128318.1"
                     /translation="YNPPQIKSIRYLTSGATHMEQQLPLDTLAIRKIIPHRYPFLLVD
                     RVEELEPGERAVGYKNVSANEEFFQGHFPDYPVMPGVLIVEAIAQVGAVALLSLGEYE
                     GQIAFFAGIDKVRFKRQVKPGDTLKLEVQLKQVRRGIGSGEGVATVDGEVACKGEFMF
                     ALAADPNRS"
BASE COUNT         2624 a         4665 c         4728 g         2766 t
ORIGIN      
        1 tctcgaagct gaggcgggta cgggtcgagg gctcgtagaa gagcgtcgcc agcacacggt
       61 cagcaaggag gtcatcagac gcgcgcaccg accgcatctc gtctgctcgg gcgaaaagct
      121 gctccaccca cgggcgctcg aactgtccca cctcgacgat gtgccgcacg gaccgactcc
      181 tcctacttcc tcgcatcagc tcgatgctgt gctaggttgc tcggatagta tacggcgcag
      241 catggaaagc ctgaggaggg gaggcgcgat gtacgagcag ctgacggatc gggtgggtgt
      301 gatgacgggt ggcgtcaaca tcggggtgat tcgaaccgaa agcggcaccg cgattctggt
      361 cgatacgggt gccaacgaag gcaacgcacg aaagatgctg cggcacgtcc gcgaggagct
      421 gtcggcagac gttgaggcga tcctgagcac gcacgggcac gcggatcact tcggagggca
      481 cgcgttcgtg gtgaagcgga ccgaggcggc cgtgtacgcc tcgcgcttcg aggcggctgt
      541 gctggagcac ccagagttgc aaccgatttt tctcttcggc ggtgccgccc ctcccgaggt
      601 gctgcgtcag cgcttcgtgt tcgcggcgcc cgtccaagtg gacgcgcatg tcgatgcggg
      661 gccgctcgag gtcggtggcg taccgatcga ggtggtgagt ctcccaggcc actcgccagg
      721 acaggtcggc tacctcgttg acggcgtctt cttctgcgcg gacgttgtct tcccgcagtc
      781 ggcaatcgac aagtaccgca tcccgtatct cttcgacctg accgggcatc tcgcctcctt
      841 ggacctggcg ctgcaggttg ccgccacggc agtcgtaccg ggacacggag tcgtggagcg
      901 cagcatcggt gaattggtgg agctcaatcg ggcggtcgcc gacgaaacgc tcgacgtcat
      961 ccggagatcg ctgaccgaac ctctggcgct ggagccgctc gcccggaggc ttttcgagcg
     1021 catggatgtc ccgatgactc ggcacgtgtc gtactacctg ctgcggccga ccatcggggc
     1081 gtacctgacg cacctcgaat cgctgggaga gattgagcac gtgatggacg gacgtatggc
     1141 gaagtggcgc ctggcgtgag acgaatcagc cgttcgaacg cagatcgctg tcgaccagct
     1201 tggcgatgtc gttaccgagg cggacactgt agttggtacc gcggtcctcg ggatagattt
     1261 gcgtcgtgtt cgccaggtaa agcccacgca gcggcgtgcg atgcggcggg atcttctcgg
     1321 agtagttgag cggcacgatc ggctgcgcgg atcgctcacg gaagacccac gtgttctgga
     1381 tccattcttc cgagaagtcg gggttcacgc gcgtgaggta ggggacgtat tcgcggatca
     1441 ggtcttcgtc cggcatgcgg aagtagggat ggtccggctc caggtacttg ctcagataga
     1501 caaaccgccg gccgccgtag gccttcggat caatgaagtt ggtgtgctcg atgatccctg
     1561 tgaagggcat gtcgtcgtcg gcgatgttga gccagtagat gtcagtgagc gggcggtcga
     1621 gctcgaggat cgcaaccacc gctgcctcgt agtggagcgt gttgagcttc gctacgtact
     1681 ccgccggcag acctggcacc atcttctgga ataccggcga cggcacggtc gcgatcagcg
     1741 cgtcgaacgt ctcctgctcg cgacggtcgg cagtggccac aatccatgtg ttcgtggccg
     1801 tcgattgccc aagatcggtg attgccgcac cgaggcgcat gtcgactcca gcctcctcac
     1861 agccgcgcac caaggcatcg accatcgtgc ggaagctgcc ctcgaagtag cccagcatct
     1921 cctgctcaag gggattggag cgagaagtgg tccgcagcca gatcttgccc cagaaccaga
     1981 ccatcgctac ccggtcgtag gccgtgccga acttggcgcg gagctgggca ccgagggtct
     2041 tgtcgtaggc gcgcttgccg agtgcccgct gcagccactc gtgcgcggtc accttctcgt
     2101 agggcttcca gtctttcttc cgctggagat agaacgtgac cgcgccgagt cgaatccggt
     2161 ctatgaaggg gattgaaccc agcctcagca catcaagtgc gccgttgagc ggatagatca
     2221 ttccatcggt gaagtatccg acgttggagg ggtgccagac gagtttgtca ccgatgccca
     2281 gctcctcggc cagatcctgt atcgcccggt cactccggaa gaggtggtgg tagaagatct
     2341 ccagttcggt gtcaccgacc ggaaacgtcc cggcttggcc gccgtactga ttgcgctccc
     2401 agagcgtgac ctgatgccct gccctcgcaa ggcgatacgc ggcggtgaga ccagcaaagc
     2461 ccccgccgat cgcgccgatg cgcttgccgt ttcctgaaat tgcgtctgat gcactcatca
     2521 ggccatcaag cctccaccgg ctctgctccc ggatcgggac gccgcgcgac gcggcggaag
     2581 agctgctgga tctcgccgtt gttccacgag acgagcagct gcgccgcatt gaagatcgac
     2641 gagtaggtgc cggcgatcat gccgatcagc agcgccagca cgaaatttct ggtcgactcg
     2701 ccgccgaaca ccagcagcgc cgccaaggtg aagattacgg tgatcgacgt gttcagcgac
     2761 cgcaccagcg tctggacaag tgaccggttc acgatctcct ccatcgaggc atcgtcatcc
     2821 atgagacggt tctcgcgcat tcggtcgaag acaacgatgg tgtcgtggac cgagaatcca
     2881 atgactgtca ggagcgcagt gacgaagagc gcgtcgatct ccacatcggc gacgacgccc
     2941 aggatggaga acacgccgag cacaaacaac gcatcgtgca gcatcgccac gatcgcggcg
     3001 gtgccataca ggagcggatt accggtgttt cgaaacgccc aggcgatgta gagcagcacg
     3061 ccgaccgacg ccgccccgac cgcaagcagc gagcggtttc ggatcgaggt accgaccgag
     3121 gcgccgatcg tctccagcgc ctccgcctcg ggatcgaggg gcgcgagcgt ctcgagggcc
     3181 tcggtgagcc gctccttttc cggcgacgac tccgccagtt cccgcagtct gatttggtag
     3241 cgattctcgt caggatcgcc cacttgctgc acctgcgcct cggtgcggcc ctccgcgact
     3301 aggacctccc ggatctcgga tgtgttcacc tcctcggtgt accggatttc ccacactgag
     3361 ccgccggtga agtcgatgcc aagctccagg ccattgaaaa tcagcgagat gatgcctggc
     3421 agcagcaaga tagctgagat caggtaccag agattgcggc ggcgggcgag gtctctcatg
     3481 cgcggcctcc cgcctcggcc gcagcgatgc gctgctggtc gggatcgatg cccagccacc
     3541 agttgctggt gaaccatccg ctctccagca ccgcctgcag gagcgtgcgg ctgacgaaca
     3601 ttgccgtgaa catgctgacc gccacgccga cgaagagtgt cagcgcaaag ccggtgataa
     3661 tcgtcgcacc agtgaactgc ccgaaccagt agaggatgac gcaggtaatc atcgtagaga
     3721 cgttggagtc acggatcgat ggccaagcgt gctcgaaccc ggcctgcacg gcgcgatcga
     3781 gccgcctccc cacgagtagc tcctctttga gacgggcgaa gatgagcaca ttggcgtcca
     3841 ccgccatgcc gattgaaagg atgaacccgg cgatgccggc aagtgtcagg gtcaccggga
     3901 tgatccggaa gagcgcgaag acgagcgcgg tgtagatcaa cagcgcgacg accgatacaa
     3961 caccggggag gcggtagaag agaatcatga agagaccgac agtcgcgagc ccgagggcac
     4021 ctgcgacaag gctcttttcg atcgacgtct gcccgagcga gggtccgatg gtgcggctcg
     4081 acacgacatc catcggtacc gacagcgcgc cggatcgcag ctggagcgcg agggtctctg
     4141 cctcctgtgc accgacgccc tcgataacgc ccgaggtaga aatcggcgcg ttgatcaccg
     4201 gggacgagat gacggtcttg tcgacgacga tcgacatggg ctggccaagg tgggtgctgg
     4261 tgtactggtg gaagtcgcgg ccggcatcac tggtcagctc gaaggccacg gcaacttggc
     4321 ccgtctgggt agtggtgaca taggcgtccg tgaggtcgga gccgctgatg atcgtctcat
     4381 agacggtgtc atctgccggc ggcggttcct ggcctgcgac cggcgtggcg agtggcactt
     4441 catcggcgct gccgagcgac gtgttcacga gcgtgccagg cggcaggtac tgaccgcggg
     4501 agtcgacaat ttcgagcagc gcagtatcct gcagcacctc gatcgcagtg tccggatcat
     4561 cgatccctgg cagctcgacg acgatctggt cactgccgcg cgtctggacg agtggctcgc
     4621 taacgccaag cgcattgacg cgtcgctcaa gggtgttgcg ggtcgcttcg agcgtccgct
     4681 cgttgacgtc gacgccttcc ggcgggcgcg cctcaagagt cacctgcagg ccaccctgca
     4741 gatcgagccc gaggcgtacg gggtgattgt cgcggtagtc agcgaagtcc caggagtcgc
     4801 ccggtagagc gatccagccg gcaacaagcg tgattagcac tacaagaacg agtctgtgcc
     4861 acagacgtaa ctgcacggtg gtctccggcg atgatggacg gtcaggggcg ggacatgatg
     4921 gagtggccgc ccgggccagt gaaacccgga aaagtataga gcaccgatca atccgttccg
     4981 tgccctaggg cataaagacc atggatgtgg atgtaggcga gcacctcggc gggcacgagc
     5041 caatccacag gctgtccggc cgcgacgcgc gcgcggatca acgtggccga gaggtcgatc
     5101 ggcacgctcg gaaaccggtc aacccgcccg gcgagctctg gcaagggact gcttcccagg
     5161 gcctcggccg cgtcccagcc gggccgctcg acgacggcga gccgcgcgag cgagagaatc
     5221 cgccggggct ggtgccaccg aggaaaatcg gtcagcgaat ccccgcccat gatgaaccag
     5281 agctggtcat ccgggtgcgc cttgcgaatg cgagcgagca aatcgctggt atacgagggg
     5341 acttcgtcgg cgaggtcaat cgggtccggg acgaacacgt cgaagccgac ggtcgcgatc
     5401 tccagcattc ggagccgatg gctgcccgac gtgactggct gtcccggctt atgcggaggc
     5461 gaccccgccg ggacaaaacg cacctcgtcg agtcccagct cgtgcttcgc gcatacagcg
     5521 atgtgcaagt gggcgatgtg taccgggtcg aaagtaccgc cgaggatgcc gatgcgtcgt
     5581 gccgccgtca cgtcaggttc acctcgatcg tgtcacccgg cagggcatcg atcaacgctt
     5641 gggaggccgc gttctccgcc tcgtcggtgt tctcaaccac aagcgaggtg gtcatggtga
     5701 gagtctcgcc cgccttggaa acgatacgcg gtgccgggag ggggcgtttg accccatcga
     5761 ccgatcccag cgcaccgtgc atgcgctcct cgatttcgtg cacggtgcgg gtctccgatt
     5821 tgatcgtgaa ctccatccgc cgcacgccgt agaatgagtc gtttcgcagg atctcggtga
     5881 agatcgtcgc gttggggatg agtacgagtt cgttgtccct attcttgacg agcgtagtgc
     5941 ggatatcgat gccctgcacc tcgccagaga catctttcac gacgatccga tcgccagatc
     6001 gaaatggtcg ctcgagaagg aggtagatgc cggcgaaaaa gttcttcagc acgtcctgga
     6061 gcgaaaggcc aatcgcgacg gtgaaggcac tgacaacggc caggaggccg gtgccggaca
     6121 caccgagcgt gttgagcacg agcagcacgg cgatgatacg gatcgctatc gatgccagac
     6181 ggctgagcag tacggtaccg ttccaaccca tcgagcggcg agacagcgtc gagcgcaccc
     6241 atgcgcgagc tagcgacccc agccaccagc caccgatcag ccagacggca atccagaagc
     6301 cgatgcgcgc aaggtcccag aggtcctcgc gcaacgcgtc atagtcaatg ctgatctccg
     6361 gcattctcgc tcctggttcg ccacttttct cggaatcagg cgtcaggcat actcggcgga
     6421 gtacgagact gtccatcggg tatcatcgca caccgcggag gtgctcgagg gatgttcgac
     6481 ggtcacgtcc actcggcgca ctccgtcgat tgctacccac ccgccgaggc atcatgcaaa
     6541 gccgcgatcg atatcgccgt cacagatgtg accattacaa gccacattta cccgccgcag
     6601 cggtatgcgc ctgagttggc gatcacgagt gatccattac gctacccgga ctcccccaac
     6661 cgctcctccg aacgctgatt gcgcgcgaga cattgttcga gatcaacacc tctgggctgc
     6721 gtcaggcccg cagaacctcc atgccggccg ggcagatagt cgagctctac gttcggcttg
     6781 gggagaactc ctcgcggtcg ggagtgactc gcgtgtggtg gcgacaattg gcgctggctt
     6841 ggtacgtaca tttaccatgc tggaggtgac cgggaaccgc gagatcacgc ggttccgaaa
     6901 tcgggttcgg gagcaggtcc caatatcaca gttccagcct tcccggcctc gactattgga
     6961 ggagaatgcc tcgcatgtcg ccccgcccga cggaggatcc tcgcgtttcg cctgagccag
     7021 ttcggcgacc ccgtcggcag ccccgccgcc gacagcgcga agaccgggta acccgatacc
     7081 tgccgtccgc gagccctgcc gccgccgagg atcgtgtcac ccgtacccgc atcgcagaag
     7141 agaggggagt tgccgccccg cggcgagcga cacccggcgc acaaccgctg gttctgcccc
     7201 attggcgaca acttgcgacg ctggcggcac ccaacgtggt ggccgctgcg tcaatcgccg
     7261 gagcggccgc ggtcacggca atcatgggtg tgttcacggc cctgttcgtg atccctctgg
     7321 tcgttcccag tctgatcctg ggcgcaatgg accggtccag gatcggacag ggatggccgg
     7381 cagcggtggg cgtgaacctt gctacgatgc tcctgctcct tcccacgctc gtcatcaggc
     7441 aaagcgctgt ccacgtgccc tacctgacga gcgagcatgg gacgatgttc gtggcggtcg
     7501 cgtcgacgat cgcggtgatc tttacgatgg tgagcattgc tctcgttgcg gcctgggcat
     7561 cacgtcagga tcccgagtcg tcaccgatgc tcttcatgcc agcagcgatg ctcgtgccgc
     7621 tgatgacgag cgccagtgaa ttcgcccagt tggatgtcgc actggccgca gctggcacaa
     7681 tcttcgcgct gacggctctc ctgacgctca tttcgtcgct gatgccgccg atctacacga
     7741 tgtttgtcgg gccgcttgcg gttgccgtgg aggtgctgtt cgtgagtttg atccgccagg
     7801 accgaatttt tccgaccggc gcgacgccga tcggtatggc gctcttcggg ttcgtcatcc
     7861 tggccgcgat cgccctgacg gtcttgctcc cggcaatgag cagctggatg cagcgggttg
     7921 agatgttccg cggccgttgg ctccgaagag ttgcgtagcc cctcaagatt catgtggtga
     7981 accgactgcc agcgcttcct ccctcgactg caagccatga ccgtgaatgt cgtccagtcg
     8041 cctcacaacg ttggttcaca cctggcgagt ccttggctgg caactcgtct cactctggta
     8101 gcgcatcacg atgcggcccg gtggagagaa gtttccgccg ggccgcacgt gcgttcgcca
     8161 ctgtgaatcc tgctagaccg tcgcgtcggg ttcggcaccg tcggcacccg gggcggattc
     8221 ctcgagatcg ccgatctgcg cctcgctggt cggatcgcgt ggctccatga tcgtcattga
     8281 ggcgacctgc tcaccaccaa ggcgcatcag cctgacgccc tgcgtggtcc gcccgatctt
     8341 cgagatttgc cccgccgcga accggattgc gatgccgcca gtgctcatca gcatcacggc
     8401 gtcgtcctcg gccaccatgc cggccgcgac gatgtccccg gtccgctcgg tgagacgcat
     8461 cgcctgaatg cccttgccac cgcgggtctg gacgcggtac tggtcgatgc tggtgcgctt
     8521 gcccatgccc tcggcactga cgacaagcag atcctcatca gtctgaggat cgatgacgtc
     8581 gaaggcgatc acggagtcgt tcccgtcgag acgaatgccg atcacgccgg cagccgtccg
     8641 acccattggc cgggcgtccg actcgtggaa ccggatcgac tggcccttct gggtgaccat
     8701 gattacgtct gaattgccgt ccgtgatccg aacccacacg agctcgtcgc tgtcatcgag
     8761 cccgatcgcc gccttgccgt cggaccgaat gcttgagaac tggtcgagcg tggtccgctt
     8821 cacgcgcccc ttccgagtcg cgaagaagag gtagttaccg ggatcgtggt ggcgcacctt
     8881 gaggagggtc gcgaccttct cgtccggctg caggttgatg acgttcacga tcggcagccc
     8941 cttcgccgtc cgcccggcat cggggagctc atgcaccttg atctggtaaa cgcgcccccg
     9001 gttcgtgaag acaagcaatg agtcgtgcgt gttggcgctc aggatgtgct ggatgccgtc
     9061 ctggtcgcgc atcgtcaggc cggtaatgcc acggccaccg cgccgctggg tgcggtagct
     9121 gtcgccgttg atgcgcttga cgtagccgcg gttggtaatg gtcaccagca cgtcgacctc
     9181 ggcgatcagg tcgtcgtcgc tcagctcgcc gctcacgttt tggatgcggg tccgccgctc
     9241 gtcgccgtac ttttccttga ggtcctccat gtcgtccttg atcaacccga ggatgagtcc
     9301 cgggttggcc agcagctcct ccagccgcga aatctcggcc atgacctcct tgtactcatt
     9361 ctcgatcttc tggcgctcga gggcggccag gcgcgcgagg cgcatgtcga ggatggcgtc
     9421 ggcttggacc cggctgagct tgaagttctt catcaggttg gagcgcgccg tatcggtggt
     9481 gcgcgagcgc ctgatagtgt tgataacctc ttcgataccg tcgagggcga tcttgaggcc
     9541 ctcgaggatg tgggcgcggt gccgggcacg ctccagctcg aactcggtcc gacgggtgat
     9601 gacctcctgg cgatgaccga cgaactcctg cagcgcccgc ttcagggtca tgacgcgcgg
     9661 ctgcatgccg cggtcgacga gcgccagcat gttgacgccg aaggtctgct gcagtgcagt
     9721 gtgcttgaag aggttgttga gcaccttctt cggctgtgcg tcccgcttga gctcgatgac
     9781 catccgcata ccggagcggt cggactcatc gcgcaggttc gagatcccgt cgagcttgcc
     9841 gtcgcggacc atttcggcga tccgctcaag cagcgcggcc ttgttcacct ggtatggcaa
     9901 ctcggtgacg acgatttggc tgcggtttcc gcgttccatg tcctcgatga aggccttggc
     9961 ccgaatcacc acccgcccgc gaccggtggc gtacgccgca tggatgccct ctcgcccgag
    10021 gatcgttccg cccgtcggga agtccggacc gggaacgatc tcgatcaact cctcaactgt
    10081 ggcgtcaggg ttgtcgatga ggtgactgac ggcgtctgcg acctcgtgga ggttgtgcgg
    10141 tggaatgttg gtcgccatgc cgaccgcgat gccggcactg ccgttgatca gcaagttcgg
    10201 gatgcgggag ggcagcaccg atggctgcat catcgtcgca tcatagttgg ggtactcgtc
    10261 gacggtgcgc ttgttgatat cgccgagcat ctcctcggcg atcgccgtca ttcgcgcttc
    10321 ggtgtagcgc atcgcggcgg cgctgtcgcc atcgaccgag ccgaaattgc cctgaccgtc
    10381 gacaagcggg taccgcaggg agaaaccctg cgccatgcga acgagcgtat cgtaggcggc
    10441 ggtatcactg tgcgggtgga agttcttgat gacctcgccg acgataccgg cgctcttgcg
    10501 gtgccgcgtg ttggagcgga ggcccatctg gtgcatggcg tagagcacgc ggcggtggac
    10561 cggcttgagg ccatcccgag cgtccggcag tgcccgctgc acgatcacgc tcatcgcgta
    10621 atcgaggtag gactgccgca tctcggactc gatgctcgca gtacgtacgt ttccaatgtc
    10681 cacgcgttga gctcctgata atgtcgagac attaggggac gccctggcgg gcgtaatgcc
    10741 taattttacc aaaatcccgg actaacggtc cgggatatgc aacataagac tacgaagggg
    10801 cgatgggcaa agctgtcaaa gcttggtgcc gcaattggtg cagaactgtg ctccagggct
    10861 gttggatgtg ccgcactccg gacagacctt cgcggcgagg ttgacgccgc attccgggca
    10921 gaacttggcg ctcgcgggga tggtggagtt gcagttgggg cactgtcggg tctcgagctg
    10981 ctgtccttgc tgctgcctct gttggctctg ctggtaggag ctgccgattg cgccggccat
    11041 cgcctgaccg atgccaagac cagcgcccag cccgacgccg gtggatgttg cgtcaccact
    11101 accgccctcg ttgcgggcag catcgccgat cgcctgcgcc gtcttgtact gcgtgtacgc
    11161 ccccatgtct ccgatggcac ccatgctcga acgctcatcg atgcgctgct gcacctcttc
    11221 gggaggagta atggcgctga tctggaagga gacgagatcg aggccgagcc gggtgaagtc
    11281 atcggccagc gccgcacgtg acgcatctgc gatctcccgg gtcatgcgct gaatgtcgag
    11341 aatcgacgtg tggacgtcac cgagcatgtc gttgaattcg ttgaggatga cgctgcgaag
    11401 gtagtcctca atcgcggcgg tgcggtacga ccctcgggtg cctaccacct cgttgacgaa
    11461 gagctgggga tcggcgacct tcatgctgta ggtcccgtag gcgcgcagcc gaaccatgcc
    11521 gagctcgctg tcgcgataga cgagcggctg ctgcgagccc cacttcatgt ccgtgaactc
    11581 ccgtaccgag acgaagtaga cctcggcgcg gaaggggctg tcgccgccga agggcaatcc
    11641 gatgagcccg gccagcagtg ggacgttgtt cgtcgaaatc gtatgtcggc ccggacggaa
    11701 gacgtcgagc gccttgccgt cgcgaaagaa caccgcgcgc tggctctcgc gcaccaccag
    11761 ctgagacccc agccggaact cgccggagcc gccctccggc acgcgataga ccaactcgtt
    11821 gctggcttca ttcgggtgtt cgatgaggtc gatgatcgcc atggctgctc ctctccaccg
    11881 aggaccgaga cacggtccgg cggaacactg ctggtcccgc cggacgccgc tgcgctacga
    11941 gttcgtgccc tggccctcgc cgagggcgcc ggcgctctgg gacgagcccg acttgagcgc
    12001 ctcaagctcc ctctcgattt cgacgtcgta gtcgagctcg cggaactggg cgtccaagga
    12061 ggtgtcctca agctccgccg tggccgccgc acgcgcctcg ttgctccgaa tcttgcgctc
    12121 catgcgctcc agctccgagg aagcgtcaag tggcgagaag gaggtcgcca ccctctctgc
    12181 cacctgctgc tgcgccctgg cgcgtcgctg ccgagcgatc aggctgtcac gctggctgag
    12241 cgtcacctgg tacttgctct ccagcttctg aagctgcgac ttgagcttct cgaccgtgtg
    12301 ctcctgcgtg gtgtgctgct cgtggtagag ctgaaggttc tcctgggcgt catgcttccg
    12361 acggagtgcc tcgcgggcga gatcgtcctt gcccgcctcc acggcacgcc gggccttcgc
    12421 ctcccactcg cggacgagct cctcggactc gttgacctcg gcctcgagct ccttctcctg
    12481 ggcgatcatt gccgcgacct gggcacgcgc cgtcgtgatg ctcgactgca tgtcacggag
    12541 aatctgatcg atcatcttct ccgggtcttc agcctgatcg atcaggtcgt tgacattcgc
    12601 tcgaacgagc cgggaaatcc gatcgagaat cgccatggtg aacaatcctt tctgtgcgcg
    12661 tcatccgccc tgactgaata tctgaagatc cagcaggggg acattcgtgc ctgaatacgc
    12721 acgatcgctg ccagccgcgc gcagtttgac ctccgcgcgt gcgtcctggc cggcgccgac
    12781 caccgacaac cgatacgtcg ccggaacgcc agaggtgctg ccctggggac ctgagatgct
    12841 cgctgatgct ggctgcccgg aggcgcccgg cgtaaccacc tcctgctcgg tcacgcgcca
    12901 ggctcgcacc tcccgaccgt cgtcggtgat tgccaaccat tcgggccctc tgtcgagctg
    12961 cactagcgtt gccactggct cacctgcagg cgaagtgagc gcgatatgcg aggtgaactg
    13021 gtagttgtcg cccagaatcg cgcccgtgcc gccgctacgg atgccaagca gctgcctctc
    13081 gtgcgcggag agtgcccgcg gctcttcgag cagcgcgagc acgtccgggt catctgacgg
    13141 ggacgccgtc tcgatcacgt cggtccgcat atcgaagagc gtgttcaggg tgtcgatctc
    13201 ccgccgaatt ccttcgagtg ccgacgggtc gaacgagtcg gcgctggcga ccgccgagac
    13261 cgactccgtc aaccccgcga cgcgctcctg aaacgccacg tcgaactggc gaatctgggc
    13321 gagcgcgtgc tcgttgacgt cgcgatccga aaagatgccc ccgtagccgt aggacgcagt
    13381 cctcacccgg ctctcgagat ggcgaaggcg ggagacagtg tcctccgccg cagagattcg
    13441 atccagctgg cgactggcgg cgagcttgct gccaatcctc gtcaggtcgg aaatcgattg
    13501 ctgcagctcc cgagcgatct gctcacgcag ccgcctgtcg tcgtcgcgca tcgattcctt
    13561 ctgccgatag ccggtgtagc ccggaacccg gttcagcagg ttctcgacgc gcgaggcaat
    13621 gcgctcggcc atggtcgtcg ttcctcctcg agtccgctag agaaagatct ccgccccgca
    13681 ataggggcac ttgatgagtc ccttgcctgc cagctccagc tgcccgccac agttcgggca
    13741 ggttgagcgc tgcgagagct cccgagcgtc gacggagtag agcatgccgc gattccagtc
    13801 gacgtagccg ctgaagaggc cacggcccac aagcgcgtgg agatgatcct gaacgttgtc
    13861 ccgggtgctg ttcatttcca gcacgacgtc atcgatgctg agctggccgc gcgtcttgac
    13921 catgtcgagc agctttcgct gcttgttgat ctccctgagc tcccgggcct cggcattgcc
    13981 gcgaatgaac agaaagatgc ctgcgccgag gagcgggagc acgatgatac cgagcaggag
    14041 ggacaaaccg agcgccgcgc cggaaccgcg caggttacct tcgcttacgc cgaacatgat
    14101 ccaggcggtg atcgcgatgc cgagcacgat gccagcaccg atcaggacga tcccgaggac
    14161 ccttccggat gctgccaact acgacctcct cgtaacccac aatggagcgg tctcgtcttc
    14221 gcccagacag gtcaattgtc gcacagggtc ccgggaagac cgtgcaccgc gtcaagaacg
    14281 gttggggtcc gcagcgagcg cgaacatgaa ctcgcccttg caggcgacct cgccgtcaac
    14341 ggtcgcgacc ccctcaccgg atccgatccc ccgtcgcacc tgcttgagct gcacctcgag
    14401 cttcagcgtg tcgcccggct tgacctggcg cttgaaccga accttgtcga ttccggcgaa
    14461 gaaagcgatc tggccctcgt actcgcccag cgagagcagg gccactgcgc caacctgcgc
    14521 gatcgcctcg acgatcagca cgccgggcat gacgggatag tccgggaagt ggccttgaaa
    14581 gaactcctcg ttggcggaga cattcttgta gccaactgcg cgctcgcccg gttcgagctc
    14641 ctcaacccgg tcaaccagca ggaagggata ccggtgcggg atgatcttcc ggatggcaag
    14701 cgtgtcgagg ggaagctgct gctccatgtg agtcgctcca gaggtgaggt aacgaataga
    14761 cttgatctgt ggaggattgt acc
//
LOCUS       DASYWL010000196         2410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_403358, whole genome shotgun sequence.
ACCESSION   DASYWL010000196 DASYWL010000000
VERSION     DASYWL010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403358"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VGR22_06860"
     CDS             complement(<1..849)
                     /locus_tag="VGR22_06860"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEV2128319.1"
                     /translation="MRSSMLGSLAVAVPRARLIGDAGTVVSDIVYHSRRVTPGALFAA
                     LRGSDFDGHDFVAAAVEQGAAALLVEEEQAIDMPQIVAQGSSRAALAQVACEFEGHPS
                     RELTVIGVTGTDGKTTTTAMLRTILVAAGYQIGTIGTVGIEIGDGTRHDLGHQTTPES
                     NLVQRFLREMAEAGTTHAVIEATSHGLATHRLDGVRFRAGGLTNITREHLEFHKTIEN
                     YRRAKAILLGRVAEERGVVVINNDDDGARSVESHAKRARIVLYSGSGEAADLRATAIR
                     AERTGLS"
     gene            complement(857..1888)
                     /locus_tag="VGR22_06865"
     CDS             complement(857..1888)
                     /locus_tag="VGR22_06865"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEV2128320.1"
                     /translation="MTTATSSPAAQWDRLLRKYGGHLLQSWAWGDLKHRHGWVPERGL
                     VQDGNGVAMAQVLFRHKGPVSIGYVPRGPLLDGDAEALWPGLMKVIDEMARRHRAISV
                     IFEPDRPLPLSGTLFDYGLVKGPEHFQPVRTVKVPLLDDEALLKQMHQKTRYSVRLAA
                     RRGVEFHTLPHSQEHIARFYELMQDTSQRNEFGVHALDYYADFLELFGDDAVLMGAWS
                     DEGNLAAMLIAGMFGNEAIYMYGGSSTKHRAHGAAFALQYEAMKWARERGAKTYDLWG
                     IPAENPDSLADSDRDRIAGTKGGDWRGLYRFKVGFGGEIVAYPPTMERRYLMLLPALA
                     RRFRALGQG"
     gene            complement(1952..>2410)
                     /locus_tag="VGR22_06870"
     CDS             complement(1952..>2410)
                     /locus_tag="VGR22_06870"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEV2128321.1"
                     /translation="HVRARALESLSVISDDESMREAIDRFYTEDETGFRATAIFAMGC
                     SADRSFLPMVLNETASDDPEIRFEAARAAGRLGDTEALPILADLADDEDAEVRHAAIN
                     ALGEIGGTAAIRYLRRIAEDAPEADYELIEDAIEEATVVIDPLLVDEGTA"
BASE COUNT          404 a          851 c          733 g          422 t
ORIGIN      
        1 ggacagtcct gtacgttcag ctcggattgc cgtcgctctg agatcggctg cctcaccaga
       61 cccactgtac aaaacaatgc gcgcacgctt ggcatggctc tcaacggacc gagcaccgtc
      121 atcgtcgttg ttgatgacaa ctacgcctcg ctcctccgcg acacgcccca ggagtatcgc
      181 cttcgcgcgc cggtagttct caatggtctt gtggaactcg agatgctcgc gggtgatgtt
      241 ggtcagtcca cctgcccgga atcgcacccc gtcgagccgg tgggtcgcga gcccatggga
      301 cgtcgcctcg atcacggcat gggtcgtgcc ggcctctgcc atctcgcgca gaaagcgctg
      361 taccagattc gactcgggcg tcgtctgatg ccccaagtca tgccgggtgc cgtcgccgat
      421 ctcgattccc accgtgccga tggtgcctat ctggtaaccg gcggcaacga ggatcgtccg
      481 cagcatggca gtcgtcgtcg tcttgccgtc cgtgccggtg accccgatta ccgtcaactc
      541 ccgagaagga tggccctcga actcgcatgc aacctgggcc agtgccgccc gcgacgatcc
      601 ctgggcgacg atctgcggca tatcgatcgc ttgctcctcc tcgacaagca gcgccgcagc
      661 gccctgctcg acggccgcag cgacgaagtc atgcccgtcg aagtcgctgc cgcgcagtgc
      721 cgcgaacagc gcccccggag tcacccgcct ggagtggtaa acgatatcac tgaccaccgt
      781 gccggcgtcg ccgatcagac gcgcgcgcgg caccgcgacg gcgagcgagc ccagcatgct
      841 gcttcgcatg tcgcgcttac ccctgcccca gggcgcggaa gcgacgagcc aacgctggca
      901 gcagcatcaa gtatcggcgc tccatcgtcg gcgggtatgc gacgatctcg ccgccgaacc
      961 cgaccttgaa gcgatagagc cctcgccagt ccccgccctt cgtaccggct atgcggtcac
     1021 ggtcgctgtc cgccagactg tccggattct cggccgggat cccccacaga tcataggttt
     1081 tcgcgccgcg ctcgcgtgcc cacttcatgg cctcatactg cagtgcgaag gccgcgccat
     1141 gtgcccggtg cttcgtggaa gagccgccgt acatgtagat cgcctcattg ccgaacatgc
     1201 cagcgatcag catcgcggcg agattgcctt catccgacca cgcgcccatc agcaccgcat
     1261 catcgccgaa cagctcgagg aaatcggcgt agtagtccag cgcgtggacc ccgaactcat
     1321 tccgctggga ggtgtcctgc ataagctcgt aaaaccgcgc gatgtgctct tgtgagtggg
     1381 ggagcgtgtg gaactcgaca ccccggcgag ccgcgaggcg cacgctgtag cgcgtcttct
     1441 gatgcatctg cttcagcagg gcctcgtcat cgagcagcgg caccttcacc gtgcgcactg
     1501 gctggaagtg ctccggtccc ttcacgagtc catagtcgaa caacgtgccg gagagcggca
     1561 acggccgatc tggctcgaag atcacgctga tggcccggtg acggcgcgcc atctcgtcga
     1621 tcaccttcat caaccctggc cacagcgcct cagcgtcacc gtcgaggagc ggcccgcgcg
     1681 gaacataccc gatgctgacc ggccccttgt gccgaaagag cacctgcgcc atggcgacgc
     1741 cgttgccgtc ctggacgagc ccacgctccg gcacccagcc gtgacgatgc ttgaggtcgc
     1801 cccaggccca ggactggagc aggtggccgc cgtacttgcg cagcaacctg tcccactgtg
     1861 cggccggact gctggtggcc gttgtcactg tagccgctat ggtgtgcccc cttggagaag
     1921 ttgaccgccc tgccggcacc ggtctctacg gtcaggcggt gccctcgtcg accaggagtg
     1981 gatcgataac caccgtcgcc tcctcgatcg cgtcctcgat cagctcataa tcggcctccg
     2041 gtgcatcctc agcgatacgg cgcagatagc ggatcgccgc ggtgccgcca atctcgccga
     2101 gcgcgttgat cgccgcgtgg cgcacctccg cgtcctcgtc gtcggcgagg tcggcaagga
     2161 tgggcagcgc ctccgtgtcg ccgagccgcc ctgccgcgcg cgcggcctcg aaccgaatct
     2221 ccgggtcgtc gctggcggtc tcattgagca ccatcggcag gaacgatcgg tccgcgctgc
     2281 agcccatcgc gaagatcgcc gtggcacgaa agccggtctc gtcctccgtg tagaaacgat
     2341 cgatcgcctc gcgcatcgac tcgtcgtcac tgatgacgga gagcgactcc agcgcccggg
     2401 cgcgaacgtg
//
LOCUS       DASYWL010000197         4409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_459795, whole genome shotgun sequence.
ACCESSION   DASYWL010000197 DASYWL010000000
VERSION     DASYWL010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459795"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VGR22_06875"
     CDS             <1..791
                     /locus_tag="VGR22_06875"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV2128322.1"
                     /translation="DIETTRISPISGGTVERASGLRTLLREDYRQQQLSLVRGRNGIL
                     IVALLLGLFSIVFTIVRRNQSTAADAAITLRLQRRTHPIFNRLMHVVSWPGFPPQSRV
                     IPPALSAMLFAFGFRLEAAFQLMAWGSSGISFFFKRIMRRSRPTTDFSGIRVAVARIG
                     GTSFPSGHVLNYLGVYGFFTYLAYTWVHPAAIRRVVVGLLGGMLALVGPSRVYLGHHW
                     FTDVTASYLLGLSWLIGLSSLYRRAKGWQYARRRRFGEKRAPLW"
     gene            1094..4261
                     /gene="ileS"
                     /locus_tag="VGR22_06880"
     CDS             1094..4261
                     /gene="ileS"
                     /locus_tag="VGR22_06880"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEV2128323.1"
                     /translation="MATESRQAFSEVSTTVDFPRLERDILRWWKERRVVEEYLHKNDG
                     SEERFSFIDGPITANNPMGVHHAWGRTYKDLFQRYHTMLGKEQRFQNGFDCQGLWVEV
                     EKELGIKNKREIEDFGIANFVERCKERVWKYSARQTEQSVRLGQWMDWDNSYYTMSDE
                     NNYTIWHMLKTCHERGWIYKGYDVMPWCPRCGTGLSEHEIVTEGYQERTHLALFVAFP
                     LQDEDASLLIWTTTPWTLAANVAVSVNPDLTYQLVERDGNRYYVAKDAAKTALKGDLE
                     VVRELQGSELVGKTYTGPFDELPAQEGTVHRVIAWDEVSPAEGTGLVHTAPGAGKEDY
                     ALSKVEGLDVIAPINEFGHYVDGFDWLSDEHVHEVGKKIEANLKEKGLLYRSEQYTHR
                     YPVCWRCGTDLVFRLVDEWFISIDGVRERLKEVTREINWYPSFGMERELDWLTNMDDW
                     MISKKRYWGLALPIWVCDDCGDFDVIGSEVELKERAVEGWEEFEGNTPHRPYIDHVKI
                     ECSACGGTASRIPDVGNPWLDAGIVALSTLDYRHDREYWRKWYPADLISESFPGQFRN
                     WFYALLTESTIVTDEKPTRNIYTYALMRDEKGEEMHKSKGNAIWFDDAAEEIGVDVMR
                     WLFATVVPQTNLNFGYGTTDEVRRRFILPLWNSYAFLVNYARLDGWTPDQQPAPEDRT
                     LLDRWILSRVNQLARYVRERIEAYDPTSAAREIEHFVVEELSNWYIRRNRRRFWKPES
                     DADKVAAYATLHEALSTVIRILAPFVPFIAEEIYQNLVGTVQPDAPKSVHLTSFPEPN
                     DALIDESLSRDMTAVLRVVRLGRAARAEANLKVRQPLPAVLVHTQDPRDAEAVVRLKD
                     QILDELNVKDVQALGELSEVVAWEIKPNLPVLGPKYGKKLGGIRKALGALDPAEVVET
                     VDAGNGVSLGEIDGEEVVLQPDEILLNLRKREGYAAAQGEGATVALDTELTEELIREG
                     LARDFVRGVQDARKRMDLRIEQKIALRYAAEGKAAEAIRANDDFIAREVLAGSIERAE
                     PLDGEVMEVKVGDTSARIFIDPLGE"
BASE COUNT          846 a         1391 c         1378 g          794 t
ORIGIN      
        1 gtgatatcga gacaacgagg atctctccaa tctccggggg aaccgtggaa cgagcatctg
       61 gattgcgaac gctgcttcgc gaagactacc ggcagcaaca gctctccctc gtgcgcggga
      121 gaaacggcat cctgattgta gcgctcctcc tcggactctt ctcgatcgtc ttcacgatcg
      181 ttcgccgcaa ccagagtaca gcggccgatg cggcaatcac ccttcgcctg cagcggcgta
      241 cccacccgat cttcaatcgg ctgatgcacg tcgtctcttg gccgggattc ccgccgcaga
      301 gccgcgtcat cccgcctgcc ctctcggcga tgctctttgc cttcggcttc cggctcgagg
      361 cggcgttcca gctcatggcg tggggctcga gcggaatttc gttcttcttc aagcgcatta
      421 tgcggcgcag tcgtcccacc accgatttct ccggtatccg cgtcgcggtc gcacgcatcg
      481 gcggcaccag ctttccgagc ggccacgtcc tcaactacct cggggtctac gggttcttca
      541 cctacctcgc ctacacttgg gtccatcccg cagcgattcg ccgtgtggtg gtcggcctgc
      601 tcggaggcat gctggcgctc gtcggcccga gccgcgtcta ccttggacac cactggttca
      661 ccgatgtcac tgcttcatac ctactcggtt tgagctggct gatcggcttg tcctcactgt
      721 atcgccgagc gaagggatgg cagtatgccc gaagacgcag attcggcgag aaacgcgcgc
      781 ccctatggta aacttgccac gatatggggc tgtgacggag acagcgtttt cgacatgggg
      841 ttcgcagcaa gtccgggagg atgggagccg gacaatcacc gatcggattc gatccctccc
      901 gagccgacgc ctgaacgcat cacatcgcaa gtaggactgt ccgggtgcgc ccgttatcgc
      961 gcgtgatgcc gctttcggca tccggagtgg cccggtaagc gccgggcaag cgaggtggta
     1021 ccgcggtcca ggccgtcctc atgtttgagg gcggtttttt cgtgagcaac atcggacaga
     1081 cgaggtgaca accgtggcaa ctgagagcag gcaggcattt tccgaggtct ccaccaccgt
     1141 cgactttccc agactcgagc gcgacatcct gcggtggtgg aaggagcggc gcgttgtcga
     1201 ggagtatctc cacaagaacg acgggtcgga ggagcggttc tcgttcatcg acggtccgat
     1261 caccgcgaac aacccgatgg gcgttcacca tgcctgggga cgcacctaca aagacctttt
     1321 ccagcgctac cacacgatgc ttggcaagga gcagcggttc cagaacggat tcgattgcca
     1381 ggggctctgg gtcgaggtcg agaaagagct cggcatcaag aacaagcgcg agatcgagga
     1441 cttcggcatc gcgaacttcg tcgagcgctg caaggagcgc gtctggaagt attccgctcg
     1501 tcagacggag cagtccgttc ggctcggaca gtggatggac tgggacaact cctactacac
     1561 gatgtcggat gagaacaatt acaccatctg gcacatgctc aagacctgcc acgagcgcgg
     1621 ctggatatac aagggctacg atgtgatgcc gtggtgcccg cgctgcggca cagggctctc
     1681 cgagcacgag atcgtcaccg agggctatca ggagcgaaca cacctcgcgc tgttcgttgc
     1741 cttcccgcta caggacgagg atgcctccct gctgatctgg accacaaccc cctggaccct
     1801 cgccgccaac gtcgccgtct ccgtcaaccc ggatctcacg tatcaactgg tcgagcgcga
     1861 tggcaatcgg tactacgtgg cgaaggatgc ggcgaagacg gcgctcaagg gcgacctcga
     1921 ggtcgtgcgt gagcttcagg gctcggagct ggtcggcaag acctacaccg ggcccttcga
     1981 cgagttgccg gcgcaagaag gaaccgttca ccgggtcatc gcgtgggacg aggtcagccc
     2041 agccgagggt acgggtctcg tgcataccgc gccgggggcc ggcaaggaag attacgcgct
     2101 ctccaaggtc gagggcctcg acgtcatcgc gccgatcaac gagttcggcc actacgtcga
     2161 cggcttcgac tggctgagcg atgagcacgt ccacgaggtc gggaagaaga tcgaggcgaa
     2221 cctgaaggag aagggactgc tctaccgatc ggagcagtat acgcaccgct acccggtttg
     2281 ctggcgctgc ggcacggacc ttgtcttccg gctcgtcgac gagtggttta tcagcatcga
     2341 cggcgtccgg gagcgcctca aggaggtgac ccgcgaaatc aactggtatc cgtcgttcgg
     2401 tatggagcgc gagcttgact ggctcaccaa catggacgac tggatgatct ccaagaagcg
     2461 atactggggt ctggcgctgc ccatctgggt ctgcgatgac tgcggcgact tcgacgtgat
     2521 cggctcggag gtggagctca aggagcgcgc cgtcgagggc tgggaggagt tcgaggggaa
     2581 cacgccacat cgcccgtaca ttgaccacgt caagatcgag tgctccgcgt gtgggggcac
     2641 tgcctcccgt atcccggacg tcggcaatcc atggctggac gccgggatcg tcgcgctttc
     2701 gacgctcgac taccggcacg accgggagta ctggcggaaa tggtatccgg cagatctcat
     2761 ctccgagagc ttcccggggc agttccgcaa ctggttctat gccctgctga ccgagagcac
     2821 catcgtcact gacgaaaagc cgacccggaa catctacacc tacgccctca tgcgcgacga
     2881 gaagggcgag gagatgcaca agagcaaggg caatgcgatc tggttcgacg atgccgccga
     2941 ggagatcggc gtcgacgtca tgcgctggct cttcgcgacc gtggtgccgc aaaccaacct
     3001 caacttcggc tacggcacca ccgatgaggt tcgccgccgg ttcatcctgc cgctctggaa
     3061 ctcctacgcc ttcctggtca actacgcccg gctcgacggc tggacgcctg accagcagcc
     3121 agcccctgag gatcggaccc tgctcgatcg ctggatcctc tcccgtgtca accaactcgc
     3181 acgctacgtg cgggagcgca tcgaggcgta cgaccccacc tcggccgctc gtgagatcga
     3241 gcacttcgtc gtcgaggaac tctccaactg gtacatccgg cgcaaccgcc gacgcttctg
     3301 gaagccggag agcgatgccg acaaggtcgc tgcctacgcg acccttcacg aggcactcag
     3361 cacggtgatc cgcatcctcg cgccgttcgt gccgttcatc gcggaggaga tctaccagaa
     3421 ccttgtcggg acggtgcagc cggatgcgcc gaagtcggtc catctcacca gctttccgga
     3481 gccgaacgac gcgttgattg acgaaagtct ctcccgcgac atgaccgcgg tgctacgggt
     3541 cgtccggcta ggccgtgccg cacgagccga ggccaacctg aaggtccggc aacccctgcc
     3601 ggctgtgctg gtgcatacac aagaccctcg tgacgccgag gccgtggttc gcctgaagga
     3661 ccagatcctc gacgagctga acgtcaagga cgttcaggca ctcggagagc tctctgaggt
     3721 ggtcgcctgg gagatcaagc cgaatctccc tgtgctcggg ccgaagtatg gcaagaagct
     3781 cggcggcatc cgcaaagcgc ttggcgcgct cgacccggcg gaggtcgtag agaccgtgga
     3841 tgcgggcaac ggtgtctcgc tcggggagat cgacggcgag gaggtcgtgc tgcagccgga
     3901 tgagatcctg ctcaacctcc gcaagcgcga gggctacgcc gccgcacagg gcgagggcgc
     3961 gaccgtcgcg ctcgataccg agctgaccga agagctcatc cgggagggcc tggcgcgcga
     4021 cttcgttcga ggtgtccagg atgcgcgcaa gcggatggac cttcgcatcg agcaaaagat
     4081 cgcccttcgc tacgctgcgg aaggcaaggc cgctgaggcc atccgtgcga acgacgactt
     4141 catcgcgcgt gaggtcctcg ccggctcgat tgagcgggcg gagcctctcg acggcgaggt
     4201 gatggaggtc aaggtcggcg acacctctgc ccgaattttc atcgatcccc ttggcgaata
     4261 agacgatccc ggcgggcggg actctcacag tcccacccgc cgctctgcat gactatttct
     4321 acaaaggaca acagcaagcg tggcaaccaa actcgttatc atcgggctgc catcctcggg
     4381 gaagaccacg gttttcaacg ccctcactc
//
LOCUS       DASYWL010000198        17360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_496703, whole genome shotgun sequence.
ACCESSION   DASYWL010000198 DASYWL010000000
VERSION     DASYWL010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 17360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17360
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496703"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..2065)
                     /locus_tag="VGR22_06885"
     CDS             complement(131..2065)
                     /locus_tag="VGR22_06885"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEV2128324.1"
                     /translation="MTLSTPSPTRLTPEALLTRMRTVSQPVISPDGNEVVYVVSHVDR
                     GTMKSVSHLWLMDLATGTTRQLTHSGTVNIVPAWSSDGGRIAFISDRVGEERHAICVM
                     ECDGGEAAEVETFATKPVSVTWHPDGTALAVVTQSGKVDPDQPRVITRIDYKQDNRGL
                     INNERNQVVLVWPERGEIRQITDSTWDHAEPQWSPDGRRIAVKQSRLNGMASRLLILD
                     EEGIEIARIGTDEGTVGTWAWSPDGGTLVLDGTEVPSPQTDYYRYDVASGELTKITDD
                     APFSAFTGFPTISGPDQPVWKDDRTVLIHGLRSGASGIWQLDVQTGETERRVSWDATH
                     GGFSADRSAERIVQTVSSLETNGELVLIDMISEETTRLTHLNDALFEDLPLAHHEKVT
                     IARDGWDIDAWVLKPAGFDPSRRYPVILDVHGGPHGNYGFSFNLGAQLMAAAGYVVVA
                     ANPRGSGTYGRVFAEAVRGDWGGEDWKDLQTVLDTVLENDWADRNRTGIYGYSYGGFM
                     TSWAIGQTDRFKAAVCGAPVFNLTSFYGTSDIGHVFGPVQWGGEPAEIEQFMVERSPS
                     TWVHRSTTPTLIIHGEQDHRCPIGQGEEMFIALKKAGCEVEFVRYPGGSHLMLRGGPL
                     HHRIDYYERVIGWFDRHLSA"
     gene            complement(2168..3466)
                     /locus_tag="VGR22_06890"
     CDS             complement(2168..3466)
                     /locus_tag="VGR22_06890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEV2128325.1"
                     /translation="MTETTSGSLATVRDQVHRWLAERRDDIANLLLSFVSVPCVTGDE
                     AAVQDVIERVMRERGLGVDRWEASREETEPYLMHVGEQERYEGRPNLVATVPGTGDGR
                     SLMLQGHVDTVEIGDPALWTRAPFGQRDGDRIYGRGSVDMKGGIVSMMVARQALQELG
                     VSVRGDVLLATSVGEEDGGLGALSTILRGHRADGVIVTEPSERRLVIAQGGSLVFRIT
                     VPGKAAHGARRDEGESAVEHFIPIFQDLLAWERERNERLSHPLYDHLGNKFPISVGLV
                     HAGNWASTVPESLVAEGRLGFLPGETIEEMQHATEARVAAVAKEDPWLREHPPTVEWF
                     GGQFASAEIAPDHPLSQTVARAHQAAHGEPVEIEGVTYGADMRLFIDIGEMPTVIYGG
                     GDVRLAHAVDEYIELDEVLGAAETLALAICDWCGYDAPVS"
     gene            complement(3643..4632)
                     /locus_tag="VGR22_06895"
     CDS             complement(3643..4632)
                     /locus_tag="VGR22_06895"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEV2128326.1"
                     /translation="MADKPQVAVTRVIPDAGLDLLRDAAEIRLWENELPPSPEKLDTL
                     LEGADGAVTLLTDRIDGPVLDRHPQLKVVSNFAVGFDNVDVPAATERGVAVCNTPNVL
                     NDATAEMAFTLMTAAARRVVEAADYVRDGNWKTWGPKLLLGQDLTGATLGIVGFGRIG
                     KKLARMVSGFDMTVLVNDVYHDPQAEQEFGVTYVSLDELLERSDFVSLHVLLNEDTHH
                     LIGTDQLKRMKQTAVLVNAARGPVVDTDALVQALQAGEIFAAGLDVTDPEPLPADHPL
                     VSLPNCTVVPHIASATVTSRDNMAKLAVNNLLDVLNRREPHAIVNPEVLRANA"
     gene            complement(5073..5876)
                     /locus_tag="VGR22_06900"
     CDS             complement(5073..5876)
                     /locus_tag="VGR22_06900"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEV2128327.1"
                     /translation="MELLTDPQAWTAFLTLTVLEIVLGIDNIVFISILTDRLPHNQRD
                     RARVVGLGAAMIMRILLLFAISWIIGLTEPWFELFGREFSGRDLILLTGGLFLLYKAT
                     TEIHAKLEGEEHDEGTAAAASFAAVIAQIMMLDIVFSLDSVITAVGMADDIEVMIAAV
                     VVAVGVMLWASGPLANFVQEHPTIKILALSFLILIGMSLVAEGFHQHIPKGYIYAAMT
                     FSVIVELINLRVRATEEKEAEPVHLKRSYAGEKAVGRAARGRTARGAGD"
     gene            6224..7237
                     /locus_tag="VGR22_06905"
     CDS             6224..7237
                     /locus_tag="VGR22_06905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEV2128328.1"
                     /translation="MSVPMWVWAATVIGVAALLIFDFYSHVKTPHEPTLKEAGIWSTV
                     YIAGALVFGAGLWWVWGSDHGLEYFAGFLTEKSLSVDNLFVFLLIMSTFAVPRQYQQK
                     VLLIGVAIAIVMRGVFIALGAAIIENFSWIFYIFGIFLLYTGWKLMESPEEAEEEYEE
                     NAFIRLMRRIWPVTDGYVEDKMFLVRHGRRMITPMAIVMLAIGSTDLLFAFDSIPAIF
                     GLTQEPYIVFTANAWALLGLRQLYFLLGGLLERLVYLAYGLAFILIWIGIKLIIHALH
                     ENEVPFINDGEHVTIIPEIPTWLSLIVITLTIALAAIFSIRKTNREKPDVNAALDIPS
                     EGD"
     gene            complement(7420..7857)
                     /locus_tag="VGR22_06910"
     CDS             complement(7420..7857)
                     /locus_tag="VGR22_06910"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2128329.1"
                     /translation="MTHDAAGTEIRLEHRVPSPEEHRRIAESVGWARGSDWPSVPRSL
                     ERSLFGVVAMTGDRAVGMGLLVGDGVLYFYIQDLAVNPAWRGRGVGQMIVETLLAYIR
                     EVTPAAAFVGLFATPDALQLYQRNGFSDGDMTGMFRLVRPDTA"
     gene            7957..8403
                     /locus_tag="VGR22_06915"
     CDS             7957..8403
                     /locus_tag="VGR22_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128330.1"
                     /translation="MAAIVPIIGVERLEDALRFYARIPGFSIAWVHRRGDMGPATMAG
                     LAWHGCAFVLVDDRCGEVPHAAGQRAIQITSRCCEQLTRSLTTVHSASRLTRPDVAWT
                     DALVAIVQDPFGIEWHLFDEDAWMRETGGPAHSYVPIRPKTGVIAP"
     gene            8400..8912
                     /locus_tag="VGR22_06920"
     CDS             8400..8912
                     /locus_tag="VGR22_06920"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV2128331.1"
                     /translation="MNTVLESVIEHHVWANDTLLASCEGLSEAQLSLTVPGTFGSVMK
                     TIVHIAEAEQIYLSRIPDTGVVHALDDEAEHLPPVADVRKALRETGQAWRAVVERWPD
                     DRPVTYARHGQEEHRSVSFFLVQMLDHGAEHRNHIRTILSAHGVEPPEIDGWEWDDAR
                     RGGATGRNPT"
     gene            9097..9537
                     /locus_tag="VGR22_06925"
     CDS             9097..9537
                     /locus_tag="VGR22_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEV2128332.1"
                     /translation="MTAGSKGTFRNTTQRSVILRVVEEADGHLTAAEIYERVRREYPS
                     IAYGTVYRTLHLLAEHELIQELTFADQASRFDKRAERHDHVHCSVCGAIEDVDVPVAL
                     LAQHVAVERSGFAIDSHHTVFVGRCPECQAAQPRERAPWARATT"
     gene            9643..10458
                     /gene="sufC"
                     /locus_tag="VGR22_06930"
     CDS             9643..10458
                     /gene="sufC"
                     /locus_tag="VGR22_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEV2128333.1"
                     /translation="MADTQKVTMFEIENLHASIEDKEILKGVALTINQGEIHALMGPN
                     GSGKSTLANVLAGHPAYEVTEGSARFMGEDILEMEPDERAQLGIFLAFQYPTVIPGVS
                     MANFLRMAVTNVRNARAERAAESGGEEVKKMTPREFRRLMKEKMRVLKMDESFATRYL
                     NEGFSGGEKKRAEILQMALLEPSMAILDETDSGLDIDALRVVSEGVNALAGDHMGILL
                     ITHYQRLLNYIQPDFVHIMMDGRVVQSGGPELAHELEAKGYETLRAEVAGATA"
     gene            10574..11983
                     /gene="sufB"
                     /locus_tag="VGR22_06935"
     CDS             10574..11983
                     /gene="sufB"
                     /locus_tag="VGR22_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEV2128334.1"
                     /translation="MVATPKPQVDIGDYKYGFRDEEDYVFKSKKGLNREVVEEISKMK
                     GEPDWMLEFRLKSLEHFQKRPMPNWGSPALHELDFEDIYYYIRPTEKQGRTWDEVPAY
                     IKDTFEKLGIPEAERKFLAGVGAQYESEVIYHSLREDLEKQGVVFLDMDAAVREHPDL
                     VQQYFGTVIPYADNKFAALNSAVWSGGSFIYVPEGVHVEVPLQAYFRINAENMGQFER
                     TLIICAPGSYVHYVEGCTAPTYNTNSLHSAVVEIVVQEGARCRYTTIQNWSKNVFNLV
                     TKRAAAYKDATMEWVDGNLGSRVTMKYPAVWLMEPGARGEVLSVAFASDGQHQDAGAK
                     MVHAAPNTSSQITSKSISKGTGRSSYRGLVKVHPGAENVRSNVICDALLLDETSKTDT
                     YPYMEIEEESVSIGHEATVSKVAEEQIFYLQSRGLSEAEAMSMIVNGFIEPIAKELPL
                     EYAVELNRLIQLEMEGSVG"
     gene            12054..13400
                     /gene="sufD"
                     /locus_tag="VGR22_06940"
     CDS             12054..13400
                     /gene="sufD"
                     /locus_tag="VGR22_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEV2128335.1"
                     /translation="MEATATKTPELTGFSREAVEALSRSRNEPDWVREARLKAWETYE
                     SIPMPKRSDEEWRRTDLRRLKLDRFVPFADVESEVTDLGTLTAGAEAMQFGETDQRAG
                     VYATRDASKPYVDVDPALAAQGVVFTDLNTAVHEHPALVQEYFMTRAVPAEFGKFEAL
                     HAAMWQGGAFLYVPKGVTVELPFRAFAFGQQAGSSIFTHSLVVLDEGAEAFFVDAYAS
                     ETQDEASFASAVVELILADDAKLRYVQVQDWGRNVWNFMTERSILGRDAEMKNLQVTL
                     GSKFSKNSVGSHLRGENALAEMLGIFFADGDQFFDHHTWQLHESPYATSDLEYKGALK
                     EKSRSVYSGLIKVYEGAQRTDAYQQNRNLVLSRTARADTIPNLEIEANDVRCTHGATV
                     SQVEDEHLFYLMARGIPKAEAQKLIVEGFFRPVIDRIPVEEIRDFLQGAIARKVGY"
     gene            13538..14767
                     /locus_tag="VGR22_06945"
                     /pseudo
     CDS             13538..14767
                     /locus_tag="VGR22_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641785.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
     gene            14861..15268
                     /locus_tag="VGR22_06950"
     CDS             14861..15268
                     /locus_tag="VGR22_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEV2128336.1"
                     /translation="MSIGGDSIYREIILDHYQNPRNRGTLDPNDYTYEDNNPLCGDEV
                     RIDVRVKDDTIDEIAFSGQGCAISQASASILMEIVDGKTLDEVKAIDKEELLDELGVD
                     LSPARLKCALLSLKVLKAALYGIDHANEELDEV"
     gene            15421..15741
                     /locus_tag="VGR22_06955"
     CDS             15421..15741
                     /locus_tag="VGR22_06955"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEV2128337.1"
                     /translation="MTETATYHTVAKVSDIEPGELMYVEAGDAFICLINLDGEIHAID
                     DTCTHEEASLSDGTIQGDEIECPLHGGAFNIRTGEPTAFPVVVPVEKYDVRIEGDEIQ
                     VKVP"
     gene            complement(15766..16797)
                     /locus_tag="VGR22_06960"
     CDS             complement(15766..16797)
                     /locus_tag="VGR22_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEV2128338.1"
                     /translation="MQQDPSDADLRNTEAYYHVVLERIPPRAEPAFESAQMQQRVWGR
                     EWGVSNDVGRLRLCVVHRPEDEITTIDASQYDPDLDALINDREQWYWRDRTGPDLKRM
                     QHEHDALVAALRDEAVEIECVGGSPGDPKAVFTRDQAIGVKGGAIICRMGPIGEEPGY
                     GRRGEEQYITRLIAGLGMPILRTIHGTGLFEGGSFCFLNADVALAGLSHRQNEAGACQ
                     IEQVLAELGVRLLRVPLTGHALHIDGGIVMVDYDKALVNMTRLPYWLPDELTGLGIEA
                     IHAWPTEPWAVNCLAVRPGRVIIPDDCPRTVERLQRAGVETIEIPYSEIRKNGGGIHC
                     STLPLIRDA"
     gene            16988..17305
                     /locus_tag="VGR22_06965"
     CDS             16988..17305
                     /locus_tag="VGR22_06965"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEV2128339.1"
                     /translation="MAKVFHPVARVDEIAPGELRYVEAGGRAICLANVGGEIVALGNT
                     CTHERASLSEGTLRGQRLQCPLHGGAFNVFSGEPERYPAVFPVKTYSVRVEDGVILVG
                     VRS"
BASE COUNT         3112 a         5472 c         5788 g         2988 t
ORIGIN      
        1 ggagggccgg cctccctgtg ggaggccggc tcctgcgaat ggggcggaca tggagtcggt
       61 ccgtatgggt tacgggtttg tgcattggct gggacaggta tcgcgcgatc gtccatgaat
      121 gagcaggtga ttacgcgctc aggtggcggt cgaaccagcc gatgacgcgc tcgtagtaat
      181 cgatgcggtg atggagcggg ccaccgcgca gcatcaggtg tgagccgccc gggtagcgca
      241 cgaactcgac ctcgcagccg gccttcttca gcgcgatgaa catctcctca ccctggccaa
      301 tcgggcagcg gtggtcctgc tcgccgtgga taatcagcgt cggcgtagtc gaccggtgaa
      361 cccaggtcga tggtgagcgc tcgaccatga actgctcgat ctccgccggc tcgccgcccc
      421 actgcaccgg cccgaagacg tgaccgatgt cgctggtgcc gtagaacgac gtcaggttga
      481 agaccggtgc gccgcagacc gcggccttga agcggtcggt ttggccgatt gcccagctgg
      541 tcatgaatcc gccataggag tagccgtaga tgccggtgcg attgcggtcg gcccagtcgt
      601 tctccagcac ggtgtcgagc accgtctgca ggtccttcca gtcctcgccg ccccaatcgc
      661 cgcgcaccgc ctcggcaaag acgcgaccgt aggtgccgga gccgcgaggg ttggcggcga
      721 ccaccacgta gccggcggcg gccatcagct gcgcgccgag gttgaagctg aagccgtagt
      781 tgccgtgcgg accgccatga acgtcgagga tcaccgggta gcgccggctc gggtcgaagc
      841 ctgccggctt gagcacccag gcgtcgatgt cccagccgtc gcgtgcgatc gtgactttct
      901 cgtggtgcgc gagcggcaga tcctcgaaga gggcgtcgtt gaggtgggtg agacgtgtcg
      961 tctcctcgga gatcatgtcg atcagcacca gctcgccgtt ggtttccagc gaactgacgg
     1021 tctgcacgat gcgctcggcg ctcctgtcgg cactgaagcc gccatgggtc gcgtcccacg
     1081 agacccggcg ctcggtctcc ccagtctgga cgtcgagctg ccagataccg ctggcaccgc
     1141 tgcgaagtcc gtggatgagc accgtgcggt catccttcca gacgggctgg tcgggaccac
     1201 tgatggtcgg gaaaccggtg aaggcactga acggcgcatc gtcagtgatc ttcgtcagct
     1261 cgccgctggc gacgtcgtag cggtagtagt cggtctgcgg gcttggcacc tcggtaccgt
     1321 cgagcacgag cgtgccaccg tccggcgacc acgcccacgt gccgaccgtt ccttcgtcgg
     1381 tgccgatgcg ggcgatttcg atgccctcct cgtcgaggat gagcagccgg cttgccatgc
     1441 cgttcaggcg actctgcttc acagcgatac ggcggccgtc gggcgaccac tgcggctcgg
     1501 cgtggtccca tgtgctgtcg gtgatctgtc ggatctcgcc gcgctccggc cacacgagca
     1561 cgacctggtt gcgctcgttg ttgatgagac cgcggttgtc ctgcttgtaa tcgatgcggg
     1621 tgatgacacg cggctggtcg gggtcgacct tgccgctctg ggtgactaca gccagggcag
     1681 tgccgtccgg atgccaggtg acggagaccg gcttggtggc aaaggtctcc acctcggcgg
     1741 cctcgccacc gtcgcactcc atcacgcaaa tggcgtggcg ctcctcgccg acacggtcgg
     1801 agatgaaggc gatccgcccg ccgtcggacg accatgccgg gacgatgttg acggtgccgc
     1861 tgtgggtgag ctgccgggtg gtgccggtgg cgagatccat cagccacaga tgactgacgc
     1921 tcttcatcgt gcccctatcg acgtgggaga cgacatagac gacctcgttg ccgtcggggg
     1981 agatgaccgg ctgcgagacg gttcgcatgc gggtaagcag ggcttcaggc gtgagacggg
     2041 ttggggaggg ggtcgacaag gtcacgaaga gctccggtat tgaggggcca agacagggaa
     2101 gggcggacat ggagtccgct cgtacaggtc gaccgcgggg cggtcatgcg tcggggtagg
     2161 ccgcccccta cgagacgggg gcatcgtagc cgcaccagtc gcagatggcc agtgcgagtg
     2221 tctcggcggc accgagcacc tcgtcaagct cgatgtactc gtcgacggcg tgggcgagcc
     2281 ggacatcccc gccaccgtag atgacggtcg gcatctcgcc gatatcgatg aagagccgca
     2341 tgtcggcgcc gtaggtgacg ccctcgatct cgaccggctc gccgtgcgcg gcttggtggg
     2401 cgcgagcgac cgtctgggac agcgggtggt ctggggcaat ctcggcagag gcgaactggc
     2461 ccccgaacca ctcgacggtc ggcggatgct cgcgcagcca gggatcctcc ttggcaacag
     2521 ctgcgacccg cgcctcggtg gcgtgctgca tctcctcgat ggtctcgccg ggcaggaacc
     2581 cgaggcggcc ctcggcgacg agcgactccg ggaccgttga cgcccagttg ccagcgtgga
     2641 cgaggccgac gctgatcggg aacttgttcc ccaggtggtc gtagagcggg tgggagagcc
     2701 gctcgttgcg ctccctctcc caggcgagaa gatcctgaaa gatcgggatg aagtgctcga
     2761 cggcggactc gccctcgtcg cgcctggctc cgtgggcggc cttgccgggg acggtgatgc
     2821 ggaagacgag ggaaccgccc tgcgcgatca ccagtcgacg ctcggagggc tcggtgacga
     2881 tcacaccgtc ggcgcggtgc ccacgaagga tagtcgagag ggcgccgagt cctccatctt
     2941 cctcgcccac cgatgtcgca agcagcacat cgccacgcac ggacacgcca agttcctgca
     3001 gcgcctgccg ggcgaccatc atggagacaa tgccaccctt catgtcgacc gagccacggc
     3061 cgtagatgcg gtcgccgtcg cgctggccga acggcgcacg ggtccagagg gccggatcgc
     3121 cgatctcgac ggtgtcgacg tgcccctgaa gcattaggga gcggccgtcg ccagtgccgg
     3181 gcactgtcgc gacgaggttt gggcggccct cgtatcgctc ctgctcgccg acgtgcatga
     3241 ggtagggctc ggtctcctcg cggctcgctt cccagcggtc aacccccaag ccgcgctcgc
     3301 gcatgacgcg ctcgatcacg tcctgcacgg ctgcctcgtc gccggtgacg caggggacgc
     3361 tgacgaagga cagcaggagg ttcgcgatgt cgtcgcggcg ctcggcgagc cagcgatgaa
     3421 cctggtcgcg tacggtggcg agagagccgg aagtggtttc ggtcataggg tcctgtttcc
     3481 caatcggacg gaagtgggcg gccagtcggg ccaggcttgt cgtggttgta cagaaatcca
     3541 agcgcgtcga tgctgatcgg atgcctctcg gcggccggct gggcggacgg ccccttcgga
     3601 gatccgcttc tacggcagcc acagggccag tacatgccat gatcacgcgt ttgcgcgaag
     3661 cacctcgggg ttgacgatgg cgtgaggctc gcggcggttg agcacgtcga gcaggttatt
     3721 gacggcgagc ttcgccatgt tgtcacgcga ggtaacggtc gccgaggcga tgtgcggcac
     3781 cacggtgcag ttcggcagcg acaccagcgg gtggtcggcg ggcaggggct cggggtcggt
     3841 gacgtcgagg ccggcggcga agatctcgcc ggcctggagc gcctgcacca gggcgtccgt
     3901 gtcgaccacc gggccccgcg cggcgttgac cagcacggcc gtctgcttca tgcgcttgag
     3961 ctggtccgtg ccgatcaggt ggtgcgtatc ctcgttcagc aggacgtgca gggagacgaa
     4021 gtccgaccgc tccagcagct cgtcgagcga aacatacgtc acgccgaact cctgctcggc
     4081 ctgtgggtcg tggtagacgt cattgacgag cacggtcatg tcgaacccgc tcaccatgcg
     4141 agccagcttc ttgccgatgc ggccgaaccc gacgatgccg agcgtggcgc cggtgaggtc
     4201 ctgcccgagc agcagcttcg ggccccacgt cttccaattg ccgtcacgca catagtccgc
     4261 ggcctcgacc acacggcggg cggcggccgt catgagtgtg aacgccatct cggcggtggc
     4321 gtcgttgagc acgttcgggg tgttgcagac cgcgacgccg cgctcagtgg cggcggggac
     4381 atcgacgttg tcgaagccga cggcgaagtt gctgaccacc ttgagctgcg ggtggcggtc
     4441 gagcaccggg ccgtcaatgc gatcggtcag gagcgtcacg gcgccgtcgg cgccctccag
     4501 cagcgtgtcg agtttctccg gcgaaggcgg cagctcgttc tcccaaaggc gaatctcggc
     4561 tgcgtcccgt aggaggtcga gaccggcgtc cgggatcacc cgcgtcactg ccacctgcgg
     4621 tttgtctgcc atgtggggtc tccgttctga caacgtacgc tcatgcgcga tccatacagc
     4681 gccacttgta ccgggaaatc ggcacgaggg caacacggcc ggtgaccgag acggcgacgg
     4741 caccggttgt ctgccgtccc ggtgccgtct ggcagctaca gccgattgcc ggaagcttgc
     4801 tcgatgagga tcggtccaca aggaccagcc tcgtgtccgt ccgagcgcgg gcagacacaa
     4861 gcgtctgtgc tgagtccttc ggctgcgctc agcatgacct tcgcgaagta cttgcctccg
     4921 gcggggcaag agggctaccc gttcaagtcc tcaaggaggc ccattgatgg gacgacattg
     4981 actgcgcggc acgatagcga tgacgggcgt tcgcagcgca gccatgttgt ctcctacggt
     5041 ggcgttgtca tgcacagggc cagcagtggg actcagtccc cggcgccgcg ggccgtacgg
     5101 ccccgagcgg cacgcccgac ggctttctcg ccagcgtagc tccgcttcag gtggaccggc
     5161 tcggcttcct tttcctccgt ggcacggacg cggagattga tcagctcgac gatgaccgag
     5221 aaggtcatcg cggcgtagat gtagcccttc gggatgtgct ggtggaagcc ctcggcgacc
     5281 agcgacatgc cgatcaggat caggaagctc agcgcgagga ttttgatcgt cgggtgctcc
     5341 tggacgaagt tcgcgagtgg acccgacgcc cagagcatga cgccgaccgc gacgacgact
     5401 gccgcgatca tcacctcgat gtcgtcagcc atgccgaccg cggtgatcac cgagtcgagg
     5461 gagaagacga tgtcgagcat catgatctgg gcgatgacgg cggcgaatga cgctgcggcg
     5521 gccgtgccct cgtcgtgctc ctcgccctcg agcttggcgt gaatctccgt ggtggccttg
     5581 tacaggagga agagcccgcc ggtgagaagg atcaggtcgc gacccgagaa ctctcggccg
     5641 aagagctcga accagggctc ggtgagcccg atgatccagc tgatcgcgaa gagcagcagg
     5701 atgcgcatga tcatggccgc gccgaggccg acgactcgcg cccggtcgcg ctggttgtgg
     5761 ggcaggcggt cggtcaggat cgagatgaag acgatgttgt cgatgccgag cacgatctcg
     5821 agcaccgtca gcgtcagaaa ggcggtccac gcttgtggat cggtgagcaa ctccatcaac
     5881 agtctgtccc ctccctgatt cgatgaacgc cgctgcgcag agcgcatatt gtgcatgata
     5941 ctgaggcgcc ggagcgcgtg gcgagggctc tcctcgcaca aatcgagccg ccgtcgtgca
     6001 ccgctgctgc gctcgcggtt tgcaccgatt ttcgaatagc acgtagaata caggctgccc
     6061 agagcggggg agtattccgt ttcggcgatg tcgtcatcac ggtccatgaa ggactcggca
     6121 tcgtgcgtcg cgcatcgcgc cagaaggcgg cacggcttgt gcggggaaga gacccgggtg
     6181 gcaaggatct taccgtccca ctcaccggag gtttctttct cgtatgagtg tccccatgtg
     6241 ggtttgggct gccaccgtca ttggtgtcgc ggcgctcctc atctttgatt tctactcgca
     6301 cgtcaagacg ccccatgagc cgaccctgaa agaggccggc atctggtcta ccgtctacat
     6361 cgccggtgcc ttggtgtttg gcgccggcct gtggtgggtg tgggggagcg accacgggct
     6421 cgagtacttc gccggcttcc tgacggagaa gagcctctcg gtcgacaacc tcttcgtttt
     6481 cctgctcatc atgtcgacct tcgccgtgcc caggcagtac cagcagaagg tgttgctgat
     6541 tggtgtcgcg attgcaatcg tgatgcgcgg cgtcttcatc gcgctgggcg cggcgatcat
     6601 cgagaacttc agctggatct tctacatctt cgggatcttc ctgctctaca ccggttggaa
     6661 gctgatggag tcgccggagg aggccgagga ggagtacgag gagaacgcct tcatccggct
     6721 catgcgtcgc atctggccag tcaccgacgg ctacgtcgag gacaagatgt tcctggtgcg
     6781 ccacggtcgg cggatgatca cgccgatggc catcgtgatg ctggcgatcg gctcgacgga
     6841 cctgctcttc gcgttcgact cgatcccggc gatcttcggc ctgacgcagg agccctatat
     6901 cgtcttcacg gcgaacgcgt gggcgctgct gggcctgcga cagctctatt tcctgctcgg
     6961 cgggctgctg gagcgactcg tgtacctcgc ctacgggctg gcgttcatcc tgatctggat
     7021 cggcatcaag ctgatcatcc acgccctgca cgagaacgag gtgccgttca tcaacgacgg
     7081 cgagcatgtg acgatcattc cggagatccc gacctggctc tcgctgatcg tgatcacgtt
     7141 gaccatcgca cttgcggcga tcttctcaat ccgcaagacc aacagggaga agccggatgt
     7201 caacgcggca ctggatatcc cctcggaggg cgactagcgc cacaggcacc cctgatacga
     7261 aaaccatcga caatgccccg ggctgacgtg gccggggcgt ttcgatggtt cacccagcac
     7321 cggcccgtca tcatctggtt ctgcagctcg cgtaccgggt gatcacatgg gggttggcaa
     7381 ggactatgac gatcacggtg atggcctgta ccgcgggtgt caggcggtgt ccgggcggac
     7441 gaggcggaac atgccagtca tgtccccatc gctgaagcca ttgcgctggt agagctgcaa
     7501 cgcgtccggc gtcgcgaaga gcccgacgaa cgccgcggcc ggcgtgacct ctcggatgta
     7561 tgccagcaga gtctcgacga tcatctggcc gacgccacgt ccccgccatg ccgggttgac
     7621 ggcgagatcc tgaatgtaga agtagagcac gccgtccccg acgagcagac ccatcccgac
     7681 cgcgcgatcg ccggtcatgg cgaccacgcc gaagagtgag cgctccagcg agcgcggcac
     7741 gcttggccag tcggagccac gcgcccatcc gacgctctct gcaatgcgcc ggtgctcctc
     7801 gggactcgga acacgatgtt cgagccgaat ctctgtccct gccgcgtcgt gtgtcatcgc
     7861 agttcttcca gcatgcccgt gggcagggat tgacttcggg acacccggcc gtacaatgag
     7921 aacatctgtt cttctttgct ctcaggagtg accactatgg ctgccatcgt cccaatcatt
     7981 ggcgtcgagc gtctcgagga cgcgcttcga ttctacgcca ggattccggg attctcgatc
     8041 gcctgggtcc accgacgcgg tgacatggga ccagcgacga tggcgggact ggcatggcat
     8101 gggtgcgcat tcgtgctggt cgacgatcgg tgtggagagg tgccgcatgc tgctggacag
     8161 cgggcgatcc agatcacctc gcggtgctgc gagcagctca cgcggagtct gacgaccgtg
     8221 cacagcgcct cacggctgac gcgtccggat gtggcatgga ccgacgcgct ggtcgcgatc
     8281 gttcaagatc cattcggcat tgagtggcac ctcttcgacg aggacgcctg gatgcgggag
     8341 actggtggtc cggcacacag ctacgtgccg atccgcccta agacaggagt gatcgcgccg
     8401 tgaatactgt cctggagagc gttatcgaac accacgtctg ggcgaacgac acgcttctcg
     8461 catcttgcga agggttgagc gaggcccaac tatcgctcac cgtgcccggc accttcggat
     8521 cggtcatgaa aacgatcgtc cacatcgcag aggcagagca gatctacttg agccgaatac
     8581 cggacacggg cgtcgtgcac gcgctggatg atgaagcgga gcacctccca ccggtggcgg
     8641 acgtccggaa ggcgctccgg gaaacgggac aagcgtggcg agctgtcgtg gagcgctggc
     8701 cggatgatcg ccccgtcacc tacgccaggc atgggcagga ggagcatcga tccgtctcct
     8761 tctttttagt gcagatgctg gatcatggcg ccgagcatcg caaccatatc cggacgattc
     8821 tgtcggctca cggcgtcgag ccgcccgaga tcgatggctg ggagtgggac gatgccagac
     8881 ggggaggcgc gactggacga aatccgactt gaccagtcgg gattggtcgg cgatactcca
     8941 gaccgtggag gccggcagat gccgggtaga tagcaatccc gaccattgtg atatactttc
     9001 gatgtgtggg aatgagactc attcctattc aggattgact cctgcgacgg caggcattcg
     9061 caccaaagcg cacgagggga acagggacac cgaaccatga ccgcaggctc caagggcaca
     9121 tttcgtaata cgacccagcg ctccgtgatc ctgcgcgtgg tggaggaggc cgacggccac
     9181 ctcaccgccg cggagatcta cgagcgcgtg cggcgggagt acccgtcgat cgcctacggt
     9241 acggtctacc gcactctgca cctcctcgcc gagcacgagc tgatccagga gctgaccttc
     9301 gcggatcagg caagccggtt cgacaagcgc gccgagcggc acgaccacgt gcactgctcg
     9361 gtctgcgggg caatcgagga tgtcgacgtt cccgttgcgc tgctcgcgca gcatgtcgcc
     9421 gtcgagcgca gcgggtttgc gatcgacagc caccacacag tcttcgtggg gcggtgcccc
     9481 gaatgccaag ccgcgcagcc gcgtgagcgg gcgccgtggg cccgcgcaac cacctgacgc
     9541 ggcagacacc gggagcaccc cggcgacgcg aatcactttc ggacgcaggc gcgctcgcac
     9601 cgggcgggcg tcgcagtatc acgcaggacg agagacgtca ctatggcaga cacgcagaag
     9661 gttaccatgt tcgagatcga aaacctccat gcttccatcg aggacaagga gatcctcaag
     9721 ggcgtggccc tcaccatcaa ccagggtgag atccacgcgc tcatggggcc gaacggctcc
     9781 ggcaagagta ccctcgccaa cgtgctggcc ggccacccgg cctatgaggt taccgagggc
     9841 tcggctcggt tcatgggtga ggatatcctc gagatggagc cggacgagcg cgcccagctc
     9901 ggcattttcc tggcgttcca gtacccgacg gtgatcccgg gcgtctcgat ggccaacttc
     9961 ctccgcatgg cggtcaccaa cgtgcgaaac gcgcgcgccg agcgtgccgc tgagtcgggt
    10021 ggcgaagagg tcaagaagat gaccccgcgt gagttccgcc gtctgatgaa ggagaagatg
    10081 cgggtcctca agatggacga gtccttcgcg acccgctatc tcaacgaggg cttctccgga
    10141 ggcgagaaga agcgcgccga aatcctgcag atggcgctgc tggagccaag catggcgatt
    10201 ctcgacgaga ccgattccgg tctcgacatc gacgcgctgc gcgtggtgtc cgagggcgtc
    10261 aacgcgctcg ccggagacca catgggcatc ctgctgatca ctcactacca gcggctgttg
    10321 aactacatcc agccggactt cgtccacatc atgatggacg gccgcgtggt gcagagcggc
    10381 ggtccggaac tggcccacga gcttgaggcg aagggctacg agacacttcg cgccgaggtc
    10441 gcgggtgcta ccgcatagcc gcacgtcgaa agatgccgcc ctctgggcgg ccatagagga
    10501 ccgcccctac gaggcagatt ccgtcggcaa ccggcatgtg caacgtgcac gctgacacct
    10561 gaaggagcaa cacatggtcg caacaccaaa gccacaggtc gatatcggcg attacaagta
    10621 cggcttccga gacgaagagg actacgtctt caagtcgaag aagggcctca accgcgaggt
    10681 tgtcgaagaa atctcgaaga tgaagggcga gcccgactgg atgctcgagt tccgcctgaa
    10741 gtcgctcgag cacttccaga agcgcccgat gccgaactgg ggaagcccgg cgctccacga
    10801 gctcgacttc gaggacatct actactacat ccggccgact gagaagcagg gccgcacgtg
    10861 ggatgaggtg ccggcctaca tcaaggacac gtttgagaag ctcgggatcc ccgaggccga
    10921 gcggaagttc ctggccggtg tcggcgcgca gtacgagtcc gaggtcatct accactccct
    10981 gcgcgaggac ctcgagaagc agggtgtggt cttcctggac atggacgccg ccgttcgcga
    11041 gcacccggat ctggtccagc agtacttcgg caccgtgatc ccgtacgcgg acaacaagtt
    11101 cgcggcgctg aactcggcgg tctggtccgg cggctcgttc atttacgtgc cggagggcgt
    11161 ccacgtcgaa gtgccgctgc aggcctactt ccgcatcaac gcggagaaca tgggccagtt
    11221 cgagcgcacg ctcatcatct gcgcgccggg ctcctacgtg cactacgtcg agggctgcac
    11281 cgcgccgacc tataacacga acagcctgca ctccgcggtc gtcgagattg tggtgcagga
    11341 gggcgcgcgc tgccgctaca cgaccatcca gaactggtcg aagaacgtct tcaacctagt
    11401 taccaagcgt gccgcggcct ataaggacgc gacgatggag tgggtcgacg gcaaccttgg
    11461 ctctcgcgtg accatgaagt acccggcggt ctggctgatg gagccgggcg cccgcggtga
    11521 ggtcctctcg gtcgcgttcg cgtctgacgg tcagcaccag gacgccggcg cgaagatggt
    11581 ccacgctgcg ccgaacacct cctcgcagat cacctcgaag tcgatctcga aggggaccgg
    11641 gcggtcgtcc taccgcggcc tcgtgaaggt ccacccgggc gccgagaacg tgcgcagcaa
    11701 cgtcatttgt gacgcgctgc tgctcgacga gacgagcaag acggacacct atccgtacat
    11761 ggagatcgag gaagagagcg tctccatcgg gcacgaggcg acggtctcca aggtcgccga
    11821 ggagcagatc ttctacctcc agagccgtgg gctgagcgaa gccgaggcga tgagcatgat
    11881 cgtcaacggg ttcatcgagc cgatcgccaa ggagctcccg ctcgagtacg cggtcgagct
    11941 caaccggctg atccagctgg agatggaagg gtcggtcgga taggcccgac gccaacacgg
    12001 aacacggcct cgttccggca tgtaccggga gaaggcaacc gtgcgaggga gcaatggaag
    12061 caactgcaac gaagacacca gaattgaccg gattcagccg cgaggcggtt gaggcgctct
    12121 cccggtcccg caatgagccc gactgggtgc gcgaggcgcg cctcaaggcg tgggagacct
    12181 acgagtcgat cccgatgccg aagcgctccg acgaggagtg gcgccgcacc gacctgcgcc
    12241 ggctgaagct cgatcggttc gtgccgttcg cggacgtcga gagcgaggtc accgacctcg
    12301 gcacgctgac ggctggtgcc gaagcgatgc agttcggcga gacggatcag cgtgccggcg
    12361 tctacgccac ccgggatgcc agcaagccgt acgtcgatgt cgatccggcg ctcgccgcgc
    12421 agggtgtcgt cttcaccgac ctcaacacgg cggtccacga gcacccggca ctggtgcagg
    12481 agtacttcat gacccgggcc gtgccggcgg agttcggcaa gttcgaggcg ctccatgccg
    12541 cgatgtggca gggcggggcg ttcctctatg tgccgaaggg cgtgacggtt gagctgccgt
    12601 tccgggcgtt cgccttcggc cagcaggccg gctcgtcgat ctttacgcac tcgctggtcg
    12661 tgctcgacga gggcgccgaa gcgttcttcg ttgatgccta cgcatccgag acacaggacg
    12721 aggcgtcatt cgcctcggcg gtggtcgagc tgatcctcgc cgacgacgcg aagctgcgct
    12781 acgtgcaggt gcaggactgg ggccgcaatg tctggaactt catgacggag cgctccatcc
    12841 tcggccgcga tgccgagatg aagaacctgc aggtgacgct gggctcgaaa ttcagcaaga
    12901 actcggtcgg ctctcacctg cgcggcgaaa atgcgctggc ggagatgctt ggcatcttct
    12961 tcgctgacgg tgaccagttc tttgaccacc acacgtggca gctgcacgag tcgccgtacg
    13021 cgacgagcga cctcgagtac aagggcgcgc tcaaggagaa gtcgcgctcg gtctactcgg
    13081 gcctgatcaa ggtctacgag ggcgcgcagc ggacggacgc ctatcagcag aatcgcaacc
    13141 tggtgctgag ccgcaccgct cgggccgaca cgattccgaa cctcgagatc gaggcgaacg
    13201 acgtgcgctg tacccacggc gccaccgtct cacaggtcga ggacgagcat ctcttctacc
    13261 tcatggcccg tggtatcccc aaggccgagg cccagaagct gatcgttgaa gggttcttcc
    13321 gccccgtcat cgatcgcatt ccggtcgagg agatccggga tttcctgcag ggcgcgatcg
    13381 cccgcaaggt cgggtactag ggcctgagcg ggccgggggc ggtcgccgcg ccgctcccgg
    13441 cagttccacc gcggtggtcc aatcgcgcgg acctcgcggt tccgcatcga aggaaaggat
    13501 caccatgggt atcacgcaga tgcctccgga cacccagatg acggccctcg acgggaacgt
    13561 ggtaaaagcc gacttcccga tcctgacgca gccgcccaag ggcggcaaga agccgctcgt
    13621 ctttctcgac agcgcggcga gctcgcagaa gccgcaggtc gtgatcgacg cgttgagcca
    13681 ctactatacc cagctcaacg cgaacatcca ccgcggcgtc tacgatctct cggaggcggc
    13741 gaccgatgcg tacgagggcg cacgcaggaa gatcgcgcag ttcctcaacg cgaagagccc
    13801 gcgcgagatc atcatggtgc gcaacaccac cgaggcgatc aacctcgtgg cgaggtcctg
    13861 gggcggcgcg agtctcgaag agggtgacct gatcgtcttc accgagatgg agcaccactc
    13921 caaccttgtg ccgtggcaca tcctcgccgc ggagaagaat ctccggcagg cacacgtcca
    13981 catcgacgac gagggccggc tcgacctcga cgagtacgcc gagatcctga agcaggagcc
    14041 gaagctcgcc gccttcaccc acgtcagcaa cagcctcggc acggtcaacc ccgtcaggca
    14101 gatgatcgcc gacgcgcacg ccgtgggtgc cgtggcagtg gtggacgctg cccagagttc
    14161 accgcacctg ccgatcgacg tgcaggacct cgacgcagat ttccttgcga tcagcgggca
    14221 caagatgctc ggtccgatgg gatcgggtgc gctctacggc aagaaggtgc tgctggacag
    14281 catgcccccg tacatgggcg gcggcagtat gatccgcaag gtcttcctcg accacaccac
    14341 gtgggcggat gtcccggcga agttcgaagc cggaacgccc gccgtgggcg acacggttgg
    14401 gctgggcgtg gcgatcgact acctgaacga gctcgggatg cctcgcgtgt gggcgcacga
    14461 gcgcgacgtc gttgcatacg cgctggaggc cctccgggag gtgccgggaa tccgcatctt
    14521 cggaccgaag gacgtgcagg cgcgcagcgg cgttgtctcc ttcgcgctcg gtgacctgca
    14581 cccgcacgat gtcgcccagg tcctgaacga gcagaatgtg gcggttcgtg ccggacacca
    14641 ctgcacccaa ccgctgatgc gggcgctcgg cgtcacggcg acgactcggg ccagcttcta
    14701 catctacaac accccggagg atgtcgaccg tctcgtcgag gcgctgcatg aggcgaaccg
    14761 ggtcttcact ggcgctccat ccccggcgac ggcggtcgcc cgcaagggcg agccctgccg
    14821 caaggagtgg gacgccagac agttcgatgg ataggcatac atgagcattg gcggagacag
    14881 catctaccgc gagatcattc tcgatcacta ccagaacccg cgaaatcgcg ggacgctgga
    14941 cccgaatgac tacacctacg aggacaacaa ccccctctgc ggcgacgagg tccggatcga
    15001 cgtccgcgtc aaggacgata cgatcgacga gatcgccttt tccggccaag gctgcgcgat
    15061 cagtcaggcg tcggcgtcga tcctgatgga gattgtcgac ggcaagacgc tcgacgaggt
    15121 caaggcgatc gacaaggagg agttgctgga cgagctcggg gtcgacctga gcccggcgcg
    15181 gctcaaatgc gcgctgctga gcctgaaagt gctcaaggcg gcgctctacg gcatcgatca
    15241 tgccaatgag gagctcgacg aggtgtagga gcctcgttga cccgtagggg cggaccccgt
    15301 gagggtgtcc gcccgttaac gcgggaacgg caacctgcgg tgccggtgtc ggcgtcgcgt
    15361 ggttcgacac ccttcggggt cgatacctac ggctggttcc cgcctagcca agggacacgc
    15421 atgacggaga ctgctaccta ccatacagtc gcgaaggtga gcgacatcga gccgggcgag
    15481 ctgatgtatg tcgaggccgg ggacgcgttc atctgcctga tcaatctcga cggcgagatc
    15541 cacgcgatcg atgacacctg cacgcacgag gaggcgtcgc tctcggacgg cacgatccag
    15601 ggcgacgaga tcgagtgccc gctgcacggc ggggcgttca atatccgcac cggggagccg
    15661 acagcgttcc cggtggtcgt gcccgtcgag aagtacgatg tccggattga gggcgacgag
    15721 atccaggtca aggtgccgta atggagcccc acgtggctcc gccgcctacg cgtcgcgtat
    15781 cagcggcagc gttgagcagt ggattccccc gccgttcttg cggatctcgg agtatggaat
    15841 ctcgatggtc tcgacgcccg cgcgctggag ccgctcgacg gtgcgaggac aatcgtccgg
    15901 gatgatgacc cggcccggcc gcacggccag acagttgacc gcccacggct cggtcggcca
    15961 cgcgtgtatc gcctcgatgc ccagtccggt gagttcgtcc gggagccagt acggcaatcg
    16021 cgtcatgttc acgagcgcct tatcgtagtc aaccatgacg atgccgccgt cgatatgaag
    16081 cgcgtgcccg gtgagtggca cccgcagcag acggacgccc agctcggcga gcacctgctc
    16141 gatctggcat gcaccggcct cgttctgtcg gtgtgacaag ccagcgagcg caacgtcggc
    16201 attcaggaag cagaatgatc ctccctcgaa caaccccgtg ccatgaatgg tgcgcaggat
    16261 gggcatgccc aagccggcga tgagcctggt gatgtactgc tcctctccgc gccgcccgta
    16321 gcccggctcc tcgccaattg gacccatccg gcagatgata gcgcctccct tgacgccgat
    16381 cgcctggtcc cgggtgaaca cggccttggg atcacccgga gagccgccca cgcactcgat
    16441 ctcgacggcc tcatcgcgaa gggccgcaac cagcgcgtcg tgctcgtgct gcatgcgctt
    16501 caggtcgggg ccggtacgat cgcgccagta ccactgctcc cggtcgttga tcagggcatc
    16561 caggtccggg tcgtactgcg aggcatcgat ggtggtgatt tcgtcttcgg gccggtggac
    16621 cacgcacagc cgtagccgtc caacgtcatt ggacaccccc cactcgcgtc cccagactcg
    16681 ttgctgcatc tgggccgact cgaacgctgg ttccgcccgt gggggaatgc gctcaagcac
    16741 tacatgataa taggcttcgg tattccgcag gtcggcatcg ctggggtctt gttgcatgag
    16801 acactcctgt tgtcggttga acacccgacc gcccaagggc gatccgtgtc acccgagcgg
    16861 tggccgtgca cttgaatgct atctgttgaa tcatattgca atgccggaac gtgccatagc
    16921 gagagtgggg tgtgttggca ggttctgccg atcatgacag ggttgcagcc gagagcgagg
    16981 cgcctgaatg gcgaaggttt tccatcctgt tgcacgggtc gacgagattg cgccgggcga
    17041 gttgcgatac gtcgaggcgg gcgggcgggc gatctgcctg gcgaacgtcg gcggtgagat
    17101 cgtcgcgctc ggcaacacct gcacccacga acgtgcatca ctgtccgagg gaacccttcg
    17161 cggccagcgg ctccagtgcc cgctgcacgg cggtgcgttc aatgtcttct ccggtgagcc
    17221 ggagcggtac cctgccgtct tccccgtcaa gacctacagc gtccgtgtcg aggatggcgt
    17281 catcctcgtc ggcgtccgga gctgaggcac agcggccgtt gtcaatcgtc cccgacgctg
    17341 cggcctccct ccacgccgac
//
LOCUS       DASYWL010000199         5895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_515992, whole genome shotgun sequence.
ACCESSION   DASYWL010000199 DASYWL010000000
VERSION     DASYWL010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5895
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515992"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VGR22_06970"
     CDS             complement(<1..337)
                     /locus_tag="VGR22_06970"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEV2128340.1"
                     /translation="MPHAYLAYLRRIYDLVEEILATQAQAIETAAEWMADAIAQPGVV
                     HLFGSGHSHMVAEEVFHRAGSLLPLNPMLDANLTLLGSVSATLLERTPGYGKVIVGSH
                     DIRDGEVVVV"
     gene            564..1481
                     /locus_tag="VGR22_06975"
     CDS             564..1481
                     /locus_tag="VGR22_06975"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV2128341.1"
                     /translation="MAIPTRPLGSTDVEVSILGVGGAHLSKPDESEAIRIVQEAIDGG
                     VTFMDNAWEYGDGESERRMGKALAESNYRQRAFLMTKDCAHDRRAEHSMRKLEESLQK
                     LRTDYLDLWQLHEVVWEDDPERILAPGGSLEALMKAKEQGKVRFIGFTGHKSPHLHRQ
                     LLDSGIPWETVQMPLNVFDAHFSSFQHKILPICLEQGIGVIGMKSNAGGHVQRSGSDI
                     TPEEALRYSLGLPASVVVSGMESVEILRKNMAVADEFVPYSEEELDALLERARPDAEA
                     GEFEPFKTSHAYDGAEGRKDHNYPLDAAD"
     gene            complement(1664..2098)
                     /locus_tag="VGR22_06980"
     CDS             complement(1664..2098)
                     /locus_tag="VGR22_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128342.1"
                     /translation="MPTRLKSLPSIVIITIGLMLGLVASTFTAGAQVVATSTAGTTQP
                     SVTQATPETTRAAATPEIDPLPLGTILVMTTEILILRAPPMTDAEALRTLPEGTQVQV
                     ISGPVQALGYTCYEVEFLDTDVSVSGWAATNADFADPIDALD"
     gene            complement(2404..3516)
                     /locus_tag="VGR22_06985"
     CDS             complement(2404..3516)
                     /locus_tag="VGR22_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEV2128343.1"
                     /translation="MRRSLSIAIIFRALLVALLGLIAVIPATAQETGTPAAGVVDEPD
                     MTPVEVVQEVQDAVVTVINEQTVSSFGVEETQPVGSGTGFIIDEEGYIVTNWHVVTGG
                     TDFAVILADGTEVPAELIGEDPRDDLAVLKIDPSVVPDTVPLADAGQLQVGETVLAMG
                     SPLGEFENTVTAGIISSLDRDQFSAGGICLAYTNLIQHDAAINPGNSGGPLFNLAGEV
                     VGVNTLGLSDVGGRSVQGLYFAVPSDTVAAVVDQMINQGFISAPYMGITFSPINPQAA
                     AANNLPVDYGVYVEGVEPNGPAAAAGLQPNDIIVAVDGQQLDEDTTLSGILFERQPGD
                     TLNLTVLRGGQEVTLSLTLGEVPPALFEQCALPGRP"
     gene            complement(3903..5072)
                     /locus_tag="VGR22_06990"
     CDS             complement(3903..5072)
                     /locus_tag="VGR22_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEV2128344.1"
                     /translation="MRPLHPPSHRLRILFVMIAVATLVAACSPDDEDAEPDPTAVVLE
                     PTTVVLEPTAIDQQDEDTTPVEVVQEVQDAVVTVINEQTITGQLGAEETQPVGSGTGF
                     IIDEEGYIVTNWHVVTGGTDFVVILADGTEVPAELIGEDPRDDLAVVKIDPSAVPDTV
                     PLGDSSQLDVAQSVLAIGSPLGAFENTVTAGIVSSLDRDQFSTGGLCLAYTNLIQHDA
                     AINPGNSGGPLFNLRGEVIGVNTLGLSTTQDGRSVQGLYFAVPSNTVAAAVDQLISQG
                     YISAPYLGVSFAALNPRLAAANDLPVDYGIYVQHVEPGGPAAEAGLQPEDIIVSIDGQ
                     QFTADISLSSILLEYDPGDTVEVTVLRGGEEVMLSLTLGEAPPELFERCTLQGQE"
     gene            complement(5326..>5895)
                     /locus_tag="VGR22_06995"
     CDS             complement(5326..>5895)
                     /locus_tag="VGR22_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128345.1"
                     /translation="VETQSVGSGDPPVSVTTDRVILPDKRHTLTTNSDTVVTEEIMIG
                     DTIYMRGTLVSSSIYPSLDPETWISFSPEQAPEDSVLEQRVTYLTTALRYPFADVTAV
                     TRALPASPAGEVIVGDRPCKAWTFSTTDDMTEGIEYVLAFDVEGRPCQLVREGGGVTE
                     TRTWSYPDNPGPINAPEEAVRVEQFPAAP"
BASE COUNT         1000 a         1796 c         2045 g         1054 t
ORIGIN      
        1 ccacgaccac cacctcgccg tcccggatgt catgtgagcc gacaatgacc ttgccatagc
       61 cgggggtccg ctccagcagc gtggcgctga cggagccgag cagagtgagg ttggcgtcca
      121 gcatggggtt gagcgggagc aggctgccgg ctcggtgaaa gacctcctcc gccaccatgt
      181 gcgagtggcc gctgccgaag aggtgcacca cgcccggttg cgcgatggcg tccgccatcc
      241 attccgccgc cgtttcgatc gcctgggcct gggtcgccag gatctcctcg acgaggtcgt
      301 aaatgcgacg caggtaggcg agataggcat gcggcacgat gcggcctccg tggtctgcgt
      361 gagggcagag caatcacggt tgccgttgcc cgccggctgg aatcctgccc catggtgcat
      421 ccctggctgg cgcttggcaa cctgcagtag ggggtcggcg aggcaacgag tggtgacgcg
      481 tggcgccaag aggtgcgagg gtacggcggt tgcggtaggg tgaccagaat cgacggcagt
      541 ttcatgtgag gagcttgcag accatggcga ttccgaccag acctctcggc agcaccgacg
      601 tggaggtatc catcctcggc gttggtggtg cgcatctctc aaagcctgac gagtccgaag
      661 cgattcgcat cgttcaggag gccatcgacg gcggtgtcac cttcatggac aacgcctggg
      721 agtacggcga tggcgagtcg gagcggcgca tgggcaaggc gctcgccgag agcaactacc
      781 gccagcgagc cttcctgatg accaaggact gtgcacacga ccgcagggcc gagcactcca
      841 tgcgaaagct cgaggagagc ctgcagaagc ttcgaacgga ctacctcgac ctttggcagt
      901 tgcacgaggt ggtgtgggag gacgaccccg agcggattct cgcgcccggc ggctcactgg
      961 aggcgctgat gaaggcgaag gagcagggga aggttcgctt cattggcttc actggtcata
     1021 agtcgccaca tctgcaccgg caactgctcg acagcggtat tccctgggag acggtgcaga
     1081 tgccgctcaa cgtcttcgac gcgcacttct cgagctttca gcacaagatc ctgccgatct
     1141 gcctggagca gggaatcggc gtgatcggca tgaagagcaa tgccggcgga catgtgcagc
     1201 gcagcggttc agacatcacg cccgaggagg cgctgcgcta ctcgctcgga ctgcccgcgt
     1261 cggtggtggt ctccggcatg gagtccgtgg agattctgcg aaagaacatg gcggtcgccg
     1321 acgaattcgt gccgtacagc gaggaggagc tcgacgcgct gctcgaacgg gcacgaccgg
     1381 atgctgaggc cggcgagttc gagccgttca agacgtcgca tgcgtacgac ggcgcggagg
     1441 gccgcaagga ccacaactac ccgctcgacg ctgcggacta ggggatgcct gcaacgtaga
     1501 gccacggcag tgtgccggcg agggcgacca gcgccgggca gcaggtcgcc actttctcgt
     1561 ttcgggttgc gatgcgccga aaccgcttgc gacgatgcgg gcccgcccat tgcgacggcg
     1621 ctcgcgacat aggtgtttct cgtacacgct ctgccgatcg acgctagtcg agcgcgtcga
     1681 tggggtcggc aaagtccgcg ttggtcgcgg cccacccgct taccgacaca tcggtgtcga
     1741 gaaactccac ctcatagcag gtgtacccca gcgcctgtac ggggccactg atcacctgca
     1801 cctgcgtgcc ttccggcagc gtcctgaggg cctcggcgtc ggtcatgggt ggcgcgcgga
     1861 ggatgagaat ctccgtcgtc atgaccagga tcgtaccgag cggaagcgga tcaatctcag
     1921 gcgtcgctgc ggctcgcgtc gtttccggcg tggcttgggt cacgctcggc tgtgtggtcc
     1981 cagcggtcga tgtggccaca acctgggcgc ccgctgtgaa ggtgctggcg accaggccta
     2041 gcatgagtcc gatagtgata atcacgatgc ttggcaagct tttcagccga gtcggcatgt
     2101 ccggtgctcc tgatgtgggc cggtcgcgat gcgcgcggca tgtcatggat cagtgtccca
     2161 ctggtagcag acgtcttgcc acgaggggca tggctgtcgg ttggcgaagc gatcggcagc
     2221 tgtgtcaggc atcgcgatac aggcgcgagc agcaactcgt ggcgggcgtc catagccggt
     2281 tcaccttgag cggcatgatc ggcctcatgt ccgcggtccg accatgccgc tgatgtggtt
     2341 gctctgtctc tgagctgtgg cgtgggccgc cctagctgtt tggccagcgc gattcgcgag
     2401 ccgttacggc cggcccggga gggcgcactg ctcgaagagg gcgggcggga cctcacccag
     2461 cgtcagcgac agggtgacct cctggccacc gcgaagcact gtcaggttga gcgtgtcccc
     2521 gggctggcgc tcgaagagga tgcccgacag cgttgtgtcc tcatcgagct gctggccgtc
     2581 cacggcgacg atgatgtcgt ttggctggag gcctgccgcc gccgcgggtc cattgggttc
     2641 aacgccctcg acgtagacgc cgtagtcgac tggaaggttg ttagcggctg ccgcctgcgg
     2701 gttgattggc gagaaggtga tgcccatgta gggggccgag atgaagccct gattgatcat
     2761 ctggtcaaca actgcagcca cggtgtcgct cggcaccgcg aagtagagtc cctgtacgga
     2821 gcgaccgccc acgtcgctca gccccagcgt gttcacgccc acgacctcgc ccgcaaggtt
     2881 gaagaggggg ccaccggagt tgcctgggtt gatggcggcg tcgtgctgga tgaggttggt
     2941 gtaggcgagg cagatgccgc cagcgctgaa ctggtcacga tcgagcgagc tgatgatgcc
     3001 agccgtgacg gtgttctcga actccccgag cggggagccc atcgccagca cggtctcgcc
     3061 gacctgcagc tggcccgcat ccgcgagggg aacggtgtca ggcacgaccg acgggtcgat
     3121 cttgagcacg gcgaggtcgt cgcgagggtc ctcgccgatg agctcggcgg gcacctcggt
     3181 gccgtcggcg aggatgacgg cgaagtcggt gccgccagtg acgacgtgcc agttggtgac
     3241 gatgtagccc tcctcatcga tgatgaaccc ggtgccggag ccgacgggct gggtctcctc
     3301 gacgccgaac gagctgacgg tctgctcgtt gatgacggtc acgaccgcgt cctggacctc
     3361 ctgcacgacc tcgaccgggg tcatgtcggg ctcgtcgacc accccagcgg ccggggtgcc
     3421 ggtctcctgg gcggtcgccg ggatgacggc gatgagcccg aggagcgcaa cgaggagcgc
     3481 acggaagatg atggcgatcg aaagggatct ccgcatagcg aattctccga tgtgtgggca
     3541 cgcggcccgc gataggtgaa cggcggtcac tagccgacgc tgagccgcgc aactttcatt
     3601 ccagcatcgc tcccccttgg cctgatcgcg tatccggtct gggaacgggg cgccagcccg
     3661 ttcgcaggat ggcctcgttg ccctgttcct cggattcatt tgatcgcttc gagcaggcgc
     3721 cggctgctct gggagaacgg tcatcagcag gtaccggcca gactcgggtg tgcacgacca
     3781 gcgccaccgc tggacggaca tggatgcttc aaagcccgta cctatccggc tggcgtcatg
     3841 cgcccgtggc gatggcagcg cgccgtccag tgcacagtgc attcgagcaa cgaggcgggc
     3901 aattactcct ggccttgaag ggtgcatcgc tcgaagagct ccggcggggc ctcgccgagc
     3961 gtcagcgaca gcatcacctc ctcgccgccg cgcagtactg tcacctcgac ggtgtcccct
     4021 gggtcatatt cgagcaggat gctcgatagc gaaatgtcgg cagtgaactg ctggccatcg
     4081 atcgagacga tgatgtcttc gggctggagg ccggcctcgg ccgccggtcc acccggctcc
     4141 acatgctgga cgtagatgcc gtagtcgacg ggcagatcgt tggcggctgc cagtcgcggg
     4201 ttcagggccg cgaagctgac gccgaggtag ggtgcggaga tgtacccctg gctgatcaac
     4261 tggtcgactg ccgctgcgac ggtgttgctc ggcaccgcga agtagaggcc ctgtacggag
     4321 cggccatcct gagtggtcga gagtcccaag gtgttgaccc cgatgacttc tccgcggagg
     4381 ttgaacagcg gcccacccga gttgcccggg ttgatggcgg cgtcgtgctg gatgaggttg
     4441 gtgtaggcca ggcacaggcc gccggtgctg aactggtccc gatcgaggga gctgacgatt
     4501 ccggcggtga ccgtgttctc gaaggctccc agtggcgagc cgatcgccaa cacggactgc
     4561 gcgacgtcga gctggctgga gtcgcccagt ggcacagtat cgggcaccgc ggacgggtcg
     4621 attttgacga cggccagatc gtcgcgagga tcctcgccga tgagttcggc gggcacctcg
     4681 gtgccgtcgg cgaggatgac gacgaagtcg gtgccaccgg tgacgacgtg ccagttggtg
     4741 acgatgtagc cctcctcgtc gatgatgaag ccggtgccgg agccgacggg ctgggtctcc
     4801 tcggcaccga gctggccggt gatggtctgc tcgttgatga cggtcacgac cgcatcctga
     4861 acctcctgca cgacctcgac cggggtcgta tcctcgtcct gctggtcgat agcggttggc
     4921 tccagaacca cggtagtcgg ctccaggacg acggcagtcg gatccggttc agcatcttcg
     4981 tcatcaggag agcaggccgc gacgagcgtc gcgaccgcaa tcatgacgaa caggatgcga
     5041 agacggtggg atggcgggtg caacgggcgc atgcattcct tcttgtcaaa ctcggccatg
     5101 cagctcggga caatcgactg gcgatcgttg ccgtgatccg cccgcacgcc tgctgaaggg
     5161 tagtcgccac atatccgcct gtcacgggtc tgtggcggcc ccctgtggta cggtctggca
     5221 tcccccatag cacgttcttt gccacagcgg cgtccggtcc acggtcgctg cacggtctcc
     5281 gaggcgactg gcgccggctc gtgtggaggt gggagccggc cgggtctatg gagcggccgg
     5341 gaactgttca acccgcaccg cctcctcggg agcattgatc ggtccggggt tgtcgggata
     5401 gctccaggtt cgcgtctcgg tcacgccgcc gccttcgcga accagctggc acggtcgtcc
     5461 ttcaacgtcg aaggctaaga cgtattcgat cccttcagtc atatcgtccg ttgtggagaa
     5521 ggtccacgcc ttgcagggcc ggtcaccaac gatcacctca ccggccggag acgcgggcag
     5581 ggcgcgggta acagccgtca cgtcggcaaa ggggtagcgg agcgctgtcg tgaggtaggt
     5641 aacccgctgc tccagcaccg aatcctccgg ggcctgctcg ggagagaagc tgatccaggt
     5701 ctccggatcg aggctcggat agatcgacga gctcacgagc gtgccgcgca tgtagattgt
     5761 gtcgccgatc atgatctcct cggtgaccac ggtgtcactg ttggtcgtga gcgtatgacg
     5821 cttgtcgggc aggataacgc ggtcagtcgt gacgctcaca ggcggatcac cggagccgac
     5881 tgactgggtt tccac
//
LOCUS       DASYWL010000200         4872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_178216, whole genome shotgun sequence.
ACCESSION   DASYWL010000200 DASYWL010000000
VERSION     DASYWL010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4872
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178216"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="VGR22_07000"
     CDS             <1..815
                     /locus_tag="VGR22_07000"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2128346.1"
                     /translation="FGLDKPLLLQFFAYVGNVFQGDLGTSFALYPADVRGLIADALPW
                     TIALQLPAILVGWILGNALGAIAAYKGGWFDRGGFIASLFLSSMPYYAFAILLLYLVA
                     VRWELLPAAGGYSFGTEPSFSWSFITDAADHYVLPFLSLVLIFIGGQAIGMRSMAIYE
                     LSSDYVEFSRGVGISDSRITRYIFRNAMLPQVTGLALSIGSLVGGALITEVVFSYPGI
                     GSLLFGAIRQNDYPVIQGITLLIMVAVLAANFLVDIAYGFIDPRIRAAQTGT"
     gene            825..1736
                     /locus_tag="VGR22_07005"
     CDS             825..1736
                     /locus_tag="VGR22_07005"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2128347.1"
                     /translation="MSTVLPSAAPDAAIDPDAKSSILGRLHRWSSQFSPRFWFALALT
                     LAVLFFGFVGPYLTGVEDPDQIVGGLYLEPSADAWLGTDNFGQDVFTQLIYGTRTSLI
                     IGVLAGLIATLIGVVIGLSAGFYGGIVEEALMGVTNVVITIPSIVVLILLAIAIDSRS
                     IYAIALIIGITSWPWTARAVRAQASSVRTREHVDLARLTGANTFSLMVFEVLPYMLSY
                     IVMAFVLQLTSSILQEAALSMLGLGPSGSISLGIMLFWALLWESIRTGAWWAFVPPTI
                     FLTLIAFALLLLQSSLDEVFNPRLRES"
     gene            1748..2782
                     /locus_tag="VGR22_07010"
     CDS             1748..2782
                     /locus_tag="VGR22_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2128348.1"
                     /translation="MQVQDVRAYYETRHGPPIRAVDGVTLDLDHGEILGIVGESGCGK
                     STLAAVLSASGPPNLKIASGQLTLDDRETVDLARSGGTPREWRGTVVSLLPQRALNSL
                     NPTARIGDYAYDVVRAHDEKMRKREAMELAGGRMEQLGLPSRVLDHYPHQLSGGMRQR
                     VVTVIATLLNPTVLIADEPTSALDVSSQKALILMMDEMMKEGMISRIIFITHDLALLS
                     NIANRVAVMYAGEIVEIGTTREVVRRPEHPYSKALIGSTLEPDPEQRGQVIHGLQGSS
                     PDMRNPPPGCRFHPRCPYVMDVCTHEVPPRMDPEHNEATCWWVKKEAEQGHETGGINR
                     ATDERVATAP"
     gene            2748..3590
                     /locus_tag="VGR22_07015"
     CDS             2748..3590
                     /locus_tag="VGR22_07015"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2128349.1"
                     /translation="MPQTNASQPLLEVRHLTKVFGTRETEVRAVDDVSFTVRQGEIVA
                     IVGESGSGKSTLARMLLRLSEPTSGEIIMDGRDVTEIKGSKNLKPYWREVQGIFQNPM
                     ASFNQFYSVGRVLRKTLALTGEKYSKAEEREKLSEALKIVGLDPKDTIPKGAHQLSGG
                     QLQRAMIARALLVDPEILIADESTSALDASLRVAILNVLKELRDRLGMTVLFITHDIG
                     QANYIADRVFVMYQGEMVEQGAVDEVLGNPQHEYTQRLLADVPRLGGWGTEDESALEP
                     ASIG"
     gene            3899..4816
                     /gene="sigJ"
                     /locus_tag="VGR22_07020"
     CDS             3899..4816
                     /gene="sigJ"
                     /locus_tag="VGR22_07020"
                     /inference="COORDINATES: protein motif:HMM:NF007214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigJ"
                     /protein_id="HEV2128350.1"
                     /translation="MIAEAPTQDYQQLRPYLFSVAYRLTGSASDAEDLVHDAWIRYLD
                     AERPKVDSLRAWLTTTVSRLALDYLKSARVRREHYTGMWQPEPVMTAEAVGGPEEAVE
                     QREAVSLAFLTLLEQLGPEQRVVYVLREGFAMSYGEIAAHLDKSVANCRQIFRRAQQR
                     LNGRVRPSVGPDGEHAALAERFLLAISTGDAAQVAVLLTEDVVWISDSGGKRLSVQRP
                     IVSVDKVSRGWAGLARKLPQDGRYVFEPAHINGAPAIVVREFGVLDRVMAFDVFDGRI
                     AAIRVLMNPDKLRHLERRLGGQGSHDIAT"
BASE COUNT          786 a         1616 c         1602 g          868 t
ORIGIN      
        1 agttcgggct ggataagccg ctgctgctgc agttcttcgc ctacgtcggc aacgtcttcc
       61 aaggtgacct gggcacctcg ttcgcgctct accccgcgga cgtgcgggga ctgatcgccg
      121 atgccctgcc gtggacgatc gcgctgcagc tgccggcgat cctcgtcggc tggatcctcg
      181 gcaacgccct cggcgcgatc gccgcctaca agggtggctg gttcgatcgg ggtgggttca
      241 ttgcctcact cttcctctcc agcatgccct actacgcctt cgccatcctg ctgctctatc
      301 tcgtcgcggt gcggtgggag ctgctgccgg ccgcgggcgg ctactccttc ggcaccgagc
      361 ccagctttag ctggtcgttc atcaccgatg ctgcggacca ctacgtgctg ccgttcctgt
      421 cgctcgtgct catcttcatc ggcgggcagg cgatcggcat gcgctcgatg gcgatctacg
      481 agctcagctc cgattacgtg gagttcagcc gcggggtcgg gatatccgac agccgcatca
      541 cccgctacat cttccgcaac gcgatgctcc cgcaggtcac cggcctggcg ctctcgatcg
      601 gttcgctggt cggcggcgcg ctgatcaccg aggtggtctt ctcctacccg ggcatcggca
      661 gcttgctctt cggtgccatc cggcagaacg actaccccgt gatccagggc attaccctgc
      721 tgatcatggt ggcggtgctg gctgccaact tcctggtgga tattgcctac ggcttcatcg
      781 acccgcgcat ccgcgccgcg cagaccggga cctaggagca cgccatgagc accgtcttgc
      841 cttcagcggc tcccgatgcc gccatcgatc ccgacgcgaa gagctcgatc ctcgggcggc
      901 tgcaccgatg gtccagccag ttctcgccgc gcttctggtt cgcgctcgcg ctcaccctgg
      961 ccgtgctgtt cttcggcttt gtgggtccgt acctgacggg agttgaggac ccggaccaga
     1021 tcgtgggcgg cctctatctg gagccctcgg ctgacgcctg gctgggcacc gacaacttcg
     1081 gccaggatgt cttcacgcag ctcatctacg gcacccgcac ctccctcatc atcggcgtcc
     1141 tggccgggct gattgcgaca ctcatcggcg tcgtcatcgg tctctcggcc gggttctacg
     1201 gtggcatcgt cgaggaggca ctgatggggg tcaccaacgt ggtgatcacc atcccctcga
     1261 tcgtcgtgct catcctgctc gcgatcgcga tcgactcgcg atccatctac gcgatcgcgc
     1321 tgatcatcgg catcaccagt tggccgtgga cggcacgcgc cgtgcgtgcg caggccagca
     1381 gcgtgcgcac gcgcgagcat gtcgacctgg cacgactcac cggcgccaac accttctcgc
     1441 tgatggtgtt cgaggtgctg ccgtacatgc tctcctacat cgtgatggcg ttcgtgctcc
     1501 agctaaccag cagcatcctg caggaggcgg cgctgagcat gctcggtctc ggcccgtcgg
     1561 gctcgatctc actcgggatc atgctcttct gggcgctact gtgggagtcg atccggacag
     1621 gtgcgtggtg ggcgttcgtg ccgccgacga ttttcctgac cctgatcgca ttcgcgctgc
     1681 tgttgctgca gtccagtctc gatgaagttt tcaatccgcg cctgcgcgag agctagggag
     1741 agggtccatg caagtccagg acgtacgcgc ctactacgaa acacgtcatg gaccgccgat
     1801 ccgggccgtc gacggggtca cgctcgatct cgaccatggc gagatcctcg gcatcgtcgg
     1861 cgaatccggt tgtggcaagt cgaccctcgc tgccgtgctc tcggcaagcg gaccgccgaa
     1921 tctcaagatc gcgagtgggc agctgaccct tgacgaccgg gagacggtcg accttgcccg
     1981 ctccggtggg acgccaaggg agtggcgcgg aactgtggtc tcgctgctgc cccagcgcgc
     2041 gctcaactcg ctcaacccca ccgcgcgcat cggggactac gcctatgacg tagtccgtgc
     2101 ccacgacgag aagatgcgca agcgtgaggc gatggagctg gctgggggac ggatggagca
     2161 gctcgggctg ccgtcgcgtg ttctggacca ctacccgcac cagctcagcg gtggcatgcg
     2221 ccagcgcgtg gtgacggtga tcgcgaccct cctcaacccg accgtgctga tcgctgacga
     2281 gccgacctca gcgctcgacg tctcctccca gaaggcgctg atcctgatga tggacgagat
     2341 gatgaaggag gggatgatct cccgcatcat cttcatcacg cacgatctgg cgctgctgag
     2401 caacatcgcc aatcgtgtgg cggtcatgta cgccggggag atcgtcgaga tcggcaccac
     2461 gcgtgaggtc gtgcgtcggc cagagcatcc ctattccaag gcgctgatcg gctcgacgct
     2521 tgagcccgac cccgagcagc gtgggcaggt catccatggg ttgcagggca gctcaccgga
     2581 catgcggaac ccacctccgg gttgccgctt ccacccccgc tgcccatatg tgatggatgt
     2641 ctgcacgcat gaggtgcccc cgagaatgga cccggagcac aacgaggcga cctgctggtg
     2701 ggtgaagaag gaggcggagc aaggccatga aaccggcgga atcaaccgtg ccacagacga
     2761 acgcgtcgca accgctcctt gaggtacggc acctgaccaa ggtgttcggc actcgtgaga
     2821 cggaggtgcg cgccgtggac gacgtgtcgt tcacggtcag gcagggtgag atcgtcgcga
     2881 tcgtcggcga gagcggcagc ggcaagtcga cgctggcgcg gatgctgctg cggctctccg
     2941 agccaacctc cggcgaaatc atcatggacg gcagggatgt caccgagatt aagggctcga
     3001 agaacctgaa gccgtactgg cgcgaggtgc agggcatctt ccagaatccg atggcgtcgt
     3061 tcaatcagtt ctactccgta gggcgggtgc tgcgaaagac actcgcgctc accggcgaga
     3121 agtacagcaa ggcggaagag cgcgagaagc tctccgaggc gctgaagatc gtcggtctgg
     3181 acccgaagga caccatcccc aagggggcgc accagctctc cggcgggcag ctgcagcggg
     3241 cgatgatcgc gcgggccctg ctcgtcgatc cggagatcct gatcgctgac gagtcgacct
     3301 cggcgctgga cgcctcgctg cgcgtggcga tcctcaacgt cctgaaagag ctgcgcgatc
     3361 gacttgggat gaccgtgctc ttcatcacac acgacatcgg gcaggcgaac tacatcgccg
     3421 accgggtctt cgtgatgtat cagggcgaaa tggtcgagca gggggcggtc gacgaggtgc
     3481 tcggcaaccc gcagcacgag tacacccagc ggctgctggc cgacgtcccg cgcctcggcg
     3541 gctgggggac cgaagacgaa tcggcgctcg agccggccag cattggctga aaatcctcgc
     3601 cggcccaacc aacgctggtc tggtcagtgc gccgtgaaca atgcgcgcag gctggtgatg
     3661 tcgccgcggt gctccgcttc gtgctgcatg agatggtgga tcgtccatgc cgggatcgcc
     3721 cctccggtcc cgcggtggaa ccgtgacagg cctcccgtga ctggacggcg cgggggcgcc
     3781 cgtcacatga tccgcgtgcc gatcgtcttc cgggaagtga accatcaccg tgggttgacc
     3841 gtgcgcgaca atgagcccgg gacccgcgtg acgtcgcttg accaggagca gccagcacgt
     3901 gatcgccgaa gcccccaccc aggactacca gcagctccgc ccctacctct tctccgtcgc
     3961 ctaccgcctg accggcagcg cctccgacgc cgaggacctc gttcacgacg cctggatccg
     4021 ctacctcgac gccgagcggc cgaaagttga ctccctgcgc gcctggctga ccaccacagt
     4081 ctcccgcctg gcgctggact acctcaagtc cgctcgtgtc aggcgcgagc actacaccgg
     4141 gatgtggcag ccggagccgg tgatgaccgc cgaggcggtc ggcggcccgg aggaggcggt
     4201 cgagcagcgc gaggcggtct cgctggcgtt cctgacactg ctggagcagc tcgggccgga
     4261 gcagcgcgtc gtctacgtgc tgcgcgaggg gttcgcgatg tcctacgggg agatcgccgc
     4321 gcatctggac aagagcgtgg ccaactgccg gcagatcttc cggcgcgcgc agcagcggct
     4381 gaatggccgg gtgcggccga gcgtcggccc ggatggcgag cacgccgcgc tggccgagcg
     4441 cttcctgctg gcgatctcca ccggcgacgc ggcgcaggtc gccgtgctgc tgaccgagga
     4501 tgtcgtctgg atcagtgaca gcggcggcaa gcgactgtcc gtgcagcgcc cgatcgtcag
     4561 cgtcgacaag gtctcgcgcg gttgggccgg gcttgcccga aagctgccgc aggacgggcg
     4621 gtacgtgttc gagccagcgc acatcaacgg cgccccggcg atcgtcgtgc gggagttcgg
     4681 cgtgctcgac cgggtgatgg ccttcgacgt ctttgacggt cggattgccg ccatccgcgt
     4741 gttgatgaat cccgacaagc tgcggcacct ggagcggcga ctcggcggcc agggttcaca
     4801 cgacatcgcc acgtagctcc ggaccgggtc gtccgcgtcg atgccgggtg acgtccccat
     4861 agcgagccat tg
//
LOCUS       DASYWL010000201        11823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_610031, whole genome shotgun sequence.
ACCESSION   DASYWL010000201 DASYWL010000000
VERSION     DASYWL010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 11823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11823
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610031"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..1259
                     /locus_tag="VGR22_07025"
     CDS             69..1259
                     /locus_tag="VGR22_07025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV2128351.1"
                     /translation="MAERTAEAVVIGGGVNGTSIAFHLAKAGLRDVVLLERWHLGAGA
                     TGKSGALVRMHYSNLPETQLAFQSLKYFENWSDMVGGDCDFRQVGTLVFASREHRDHL
                     EANLAIQREVGVNTRLVTADDARQIDPAVDTDDVDVVGYEPESGYADPNATTYAFADA
                     ARVMGVEILQGAPALRVLTDGDRVTGVETDGGVIEAPQVIVVAGAWANQLFEPLGVDL
                     GLFPRQSRIAIFRGAQGRTYPHPTYIDHVNHLWARPIDDNCTLGGAEMDAPVHVDPED
                     YPEHVTQEYIDFTREQLRKRIPSIAQSTVRGNWACALMGSPDGRPLIGAIKQYDGLYT
                     MAGDNGTSFKTAPAIGKCLSELMLEGEATTVDLTPFRPSRIVEGQPWVDRYDYDLVDA
                     SVSR"
     gene            complement(1391..2905)
                     /locus_tag="VGR22_07030"
     CDS             complement(1391..2905)
                     /locus_tag="VGR22_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV2128352.1"
                     /translation="MSEVWKNFIGGEWVESRSGETFERRNPATGEVVGTFTKSNAGDV
                     DEAVAAASKAFRSWRLVPAPKRGEILYRTARILEERKNDLAREMTQEMGKVFTEAAGD
                     VQEAIDMTYYMAGEGRRMFGQTTPSELRNKFNMSVRKPLGVAGMITPWNFPMAIPSWK
                     MTPALVAGNTIVFKPATYTPKLAIRFVEVLQEAGIPDGVVNLVIGSGPDVGDPLVSHP
                     DVPLLSFTGSTETGTRVATEAAKMNKRVTLEMGGKNAVIVMDDADLDLATEGILWGAF
                     GTTGQRCTATSRVVVHASVKQELEDRLAARASKMQLGDGLLESTDIGPIVSDSQLQNV
                     HNYIDIGRDEGARLVTGGEIRSEGELGKGFFHEPTIFSDVSRDMRIANEEIFGPVVSL
                     IPVDGLDEAIDIANSVQYGLSAAIFTQNVNNAFRAMQDIYTGILYINAGTTGAEIHLP
                     FGGTKGTGNGHREAGTAALDFYMEWQAIYVDYSGKLQRAQIDTDKPLDEGQIAP"
     gene            complement(3049..3123)
                     /locus_tag="VGR22_07035"
     tRNA            complement(3049..3123)
                     /locus_tag="VGR22_07035"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3089..3091),aa:Asn,seq:gtt)
     gene            3241..4224
                     /locus_tag="VGR22_07040"
     CDS             3241..4224
                     /locus_tag="VGR22_07040"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128353.1"
                     /translation="MWNPNSYRDSDYGLSNDERFEPTDKLDDDFAPREGEPFIQTVLG
                     PIAPDEAGVALVREYLQWRPGGLADVQAALTDLEAFFTASGRTIVSATAPDAGRDAHV
                     LLTLAQHAPLHIIGGTGWSIVPSSEFNSRSIEEGIERDITDGMDDTPARPGLVVGSIG
                     ADMPPNVVGDALGLLASAQRRFGLPVMVDCGGRDNLKRVLESKLDVEGLIVTGDVVRT
                     QDSAIRIAEGGAFVLVSPLEGINAERDRQAARLVTALVDRGWQHRVLVSHAFTDRSRL
                     NGYGGRPGLSYLVEQFAVMLLESGMHAHDVQQIVIDNAALALSVRGAQQSA"
     gene            4249..4500
                     /locus_tag="VGR22_07045"
     CDS             4249..4500
                     /locus_tag="VGR22_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128354.1"
                     /translation="MTTRVYLAAAMFREEPMQPRDLPAERVFVNASDVKEVWVETESD
                     DQVPDIGRAVAFALVNPIEIGFRRITGTVERKIDKTRQN"
     gene            complement(4595..5410)
                     /locus_tag="VGR22_07050"
     CDS             complement(4595..5410)
                     /locus_tag="VGR22_07050"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEV2128355.1"
                     /translation="MDTRTSVGERTLAIELADQISAWLRDQLEWSGADRFVLGLSGGI
                     DSAVVAGLAARAVGSDRVFAVMMPAASNPSDLESAALVAQAFGIPATTIDLTAPTQSL
                     RAVLPSAPDVQERLGLPSAPPAEAVQLADANIKPRLRMATLYYVANLCRGIVLGTGNK
                     TEAMIGYFTKYGDGGVDLLPIVDLYKREVREVARAIGVPQQVIERPPSAGLWQGQTDE
                     DEIGITYDELDRTLEGIESGDTRDADPATVERVMQMVASSEHKRQRIPRFSRD"
     gene            complement(5474..6892)
                     /gene="lpdA"
                     /locus_tag="VGR22_07055"
     CDS             complement(5474..6892)
                     /gene="lpdA"
                     /locus_tag="VGR22_07055"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEV2128356.1"
                     /translation="MADSDRDQKYDLVLLGGGTGGYVAAIRARQLGLSVAVVEELILG
                     GTCLHRGCIPTKAMLKSAGVFDEVKRAAEFGIDIKGDVTFNYDVALKRSLGVVDSQYR
                     GLTYLFDKKHKIPVIVGRGKMLDKNTVEVTPKEGGEAFKLSGNKIIINTGSRPRAIKG
                     IEFDGKTVFNSDHAVVREDMPKSVIVRGGGATGIEWASIYHRYGAKVTMVGNVVPNED
                     KDIRAQVERSLRRQKLDVIPGARPTADDFDISDKVVKVTVKDAKGKEQSVEADTLFVA
                     IGREGNIEDIGLETVGVKTERGIIPVGEMTQTNVEGVYAIGDVNGQQMLAHTAQHQGI
                     IAVEHIAGLSPEPLDILNSPSCTYSEPEIGSVGLTEDQAKELGYEVKIGSFPMRPNAK
                     ATIEGHTEGMTKLVVDADTDDVLGVHIVGPHATELIGEAALGRLLNMSVQELGWTVHP
                     HPTVIEVIGEAAHDALGHAIHI"
     gene            complement(7152..7859)
                     /locus_tag="VGR22_07060"
     CDS             complement(7152..7859)
                     /locus_tag="VGR22_07060"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEV2128357.1"
                     /translation="MYRFMDHRRTNQTLQPVRSEARVLSHKFAADDTVMFAGENEGSL
                     FLIRDGWVRLYRILNDDRTLVLGLLGPGEAFMQDTGSDGVTPTTAADALTDCEVTEIP
                     FERVGAVLAKSPEYSRKLLSALASRQTATHDFIEQVLTRDTSVRLATILVDLAKAVGE
                     ETESEGVLRVTRSATHQALANMIGSNRVTVTRKLLDMQSRNLVRSLGRNSLEVDVPAL
                     RGFIEEANAPEENRNAA"
     gene            complement(8025..8918)
                     /locus_tag="VGR22_07065"
     CDS             complement(8025..8918)
                     /locus_tag="VGR22_07065"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128358.1"
                     /translation="MDIGSNTVKVTVYECDAGRRLRPLYGDAHTVRIGYRVSETGRIS
                     EERLERLLDVLRRYETRAVELGASQFLAVATQALRVAANSQEAVASIERETRWRVNVI
                     DADEETRLTLEGARPWIEPGVVNVVADIGGASTELIAVRADGNLESSGSVPTGSGMLF
                     DEEIGTSPPPDGSMARARERASTALGKSGLVPYSARMLLLPGGTGHYLNLLTGSLSSG
                     AELAPDRLELLADWLGSRHAIETMERIPVQFDRAQVLPASFAIVEALVLRCSPQRILA
                     IPSGIRDGVARTLCEGGQSTG"
     gene            complement(8954..9673)
                     /locus_tag="VGR22_07070"
     CDS             complement(8954..9673)
                     /locus_tag="VGR22_07070"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV2128359.1"
                     /translation="MKIAVMSDIHGFAPAFEAVLADLEHRDVEQLIVAGDLFEGGPDP
                     RRVLQLLRLHDARCVYGNTDRDMLDAGAASSGSIAWTREQIGAGGLAFLRDLPFSIRV
                     RHPAGNAGPASELLVVHANPTDVERHLSPNASDREINEILGEELAKTIAFGHLHVSYV
                     RQVGDRTLVDVSAVGNPRDGDLRPRYVVFEAVEGGGWDPTWHYVDYPLEETRGLMETS
                     GMPDWQEAYRRLESARYNRPI"
     gene            9758..10120
                     /locus_tag="VGR22_07075"
     CDS             9758..10120
                     /locus_tag="VGR22_07075"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV2128360.1"
                     /translation="MQRFRQDRTLATPSSGEIFIGKVFSQQMVTDQNAPSLRVNSLTF
                     EEGARNRWHRHSTEQVLIIMEGRGIVENESETFQVAAGDIVLIPAGERHWHGAEENAS
                     MTHLSILIPGELTIDDEG"
     gene            complement(10157..11440)
                     /locus_tag="VGR22_07080"
     CDS             complement(10157..11440)
                     /locus_tag="VGR22_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128361.1"
                     /translation="MKRQRQPLILVVMVALFALASPVSAQEAEPQEFEDITQIVADVR
                     GLEPLRPIELTPMTREQLAVELQEDLLEDYPEDEQYADERELLAFGLMDEHIDFGDLY
                     IDLYSEQIAGYYDPETSEMVVIRDATDEAGFTPSQEVTYAHEIVHALQDQHFDLDAGV
                     LDREPLSDDQALAVTALIEGDASYAELQYLMENPELLEAFLQEIEVTEFDSSVLDAAP
                     VIISNTLIFPYEHGLTFVEALYSEGGWDLVNQALADPPASTEQILHPEKYLDGGAPVE
                     IEVADFTQELGDDWTVFDTNTFGEYQLRVILGETSMGEEQAERAAAGWGGDTYLVAGT
                     DTEDAILWESAWDTPQDAEEFARAWALYETERWGVGPTYVADNVLQFETDEVVTRVIL
                     DGDEVTYAMAPNVEMLETILAAEAAPATPAATPIS"
     gene            11602..>11823
                     /locus_tag="VGR22_07085"
     CDS             11602..>11823
                     /locus_tag="VGR22_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128362.1"
                     /translation="MAERTVTRYRALAETVFSLRFEAMPVWIASDLVAWLDDRSGVMQ
                     LYAGNPITGERRQLTTGAERIQSLIGTAGS"
BASE COUNT         2094 a         3853 c         3698 g         2178 t
ORIGIN      
        1 caaccggtca ccccggtcgg ctggccgggg tttcaacact gacaactctg ggaacacagg
       61 gaggccatat ggcagagcgg acagccgaag cggtggtgat cggtggcggg gtgaacggca
      121 cgagcatcgc gtttcatctt gccaaagcag gcttgcggga tgtcgtgctg ctggagcggt
      181 ggcaccttgg ggccggcgcg accggcaaga gcggcgcgct cgtgcggatg cactacagca
      241 accttccgga gacgcagctt gccttccaaa gcctgaaata ctttgagaac tggtcggaca
      301 tggtcggcgg cgactgcgat tttcgccagg tcgggacctt ggtctttgca tctcgcgagc
      361 atcgtgacca cctggaggcc aacctcgcga tccagcggga ggtcggggtg aacacacgcc
      421 ttgtcactgc ggacgatgcc cgccagatcg acccagcggt cgacaccgac gatgtcgatg
      481 ttgttggcta cgagccggaa tcgggctatg cggacccgaa tgcgacgacc tacgccttcg
      541 ccgatgcggc gcgggtgatg ggtgtcgaga tcctccaggg tgctcccgcg cttcgcgtct
      601 tgaccgatgg cgatcgggtc accggtgttg agacggatgg aggggtgatc gaggctcccc
      661 aagtcatcgt cgttgccgga gcttgggcga accagctctt cgagccactc ggcgtcgatc
      721 tcgggctctt cccgcgacag tcgaggatcg ccatcttccg cggcgcacag gggcggacgt
      781 acccgcatcc gacgtacatc gaccacgtga accacctctg ggcccgaccg atcgacgaca
      841 actgcacgct tggcggtgcg gagatggacg cgccagtaca tgtcgatccg gaagactatc
      901 cggagcacgt aacgcaggag tacatcgact ttacccggga gcaattgcgc aagcgcatcc
      961 cgtccatcgc gcagagcact gtgcgaggca actgggcctg cgcgctgatg gggagccccg
     1021 atggccggcc cctgatcggc gccatcaagc agtacgacgg gctctacacc atggctggcg
     1081 acaacggcac ctcgttcaag acagcgccag ccatcggcaa gtgtctcagc gagctgatgc
     1141 tcgagggcga ggccacgacg gtcgatctga cgccgtttcg gccatcccga atcgttgagg
     1201 ggcaaccctg ggtcgacagg tacgactacg acctcgtcga cgcgtcggtg tccaggtagc
     1261 tcgactgcaa cgaagagggt cgacaggcac tcgacccgaa cgggcggact ccatgtccgc
     1321 caagccattg caaaaacggg aggccgttcg gcctcccgta ttctatgtcg atcgtcagca
     1381 ctgctgaaag ctacggggcg atctgacctt cgtcgagcgg cttgtcggtg tcgatctgcg
     1441 cccgctgcag cttgcccgag taatcgacgt agatcgcctg ccactccatg tagaagtcga
     1501 gggcggccgt gcccgcctcg cggtgaccgt tgccggtccc cttggtcccg ccgaatggca
     1561 gatgaatctc ggcgccggtc gtccctgcgt tgatgtagag aatgccggtg tagatgtcct
     1621 gcatcgcacg aaatgcgttg ttgacattct gagtgaagat tgccgccgac aggccgtact
     1681 ggaccgagtt cgcgatgtcg atcgcctcgt cgagtccatc taccggaatc agcgacacca
     1741 caggcccgaa aatctcctca ttggcgatcc gcatgtcgcg ggatacgtcg gagaagatgg
     1801 tcggctcatg gaagaatccc ttgccgagct ccccctcgga acgaatctcg ccgccggtga
     1861 cgagcctcgc gccctcatcc cggccgatat cgatgtagtt gtgcacgttc tgcagctgag
     1921 agtcgctcac gatcgggccg atgtcggtgc tttcaagcag gccgtcaccg agctgcatct
     1981 tactcgcccg ggccgccaat cgatcctcga gctcctgctt cacggaggcg tggaccacca
     2041 cacggctggt tgccgtgcac cgctgcccgg ttgtaccgaa cgcgccccag aggatgccct
     2101 cggtcgcgag atcgagatcg gcgtcatcca tcacgatgac ggcattcttg ccgcccatct
     2161 ccagggtgac gcgcttgttc atcttggccg cctcggtggc aacgcgggtg cccgtctcgg
     2221 tcgaaccggt gaaggagagc aggggaacgt cagggtgaga gacgagtggg tcgccaacgt
     2281 cggggccgct gccgatcacg aggttgacga cgccatccgg aatgccggct tcctggagca
     2341 cttccacgaa gcgaatcgca agcttgggtg tgtatgtggc cggcttgaag acgatcgtat
     2401 tgcccgccac gagtgcggga gtcatcttcc acgacggaat cgccatcggg aagttccatg
     2461 gcgtgatcat tcctgcaacg ccgagcggct tgcggaccga catgttgaac ttgttgcgca
     2521 gctccgacgg cgtggtttga ccaaacatgc gccggccctc accggccatg tagtaggtca
     2581 tgtcgatggc ttcctggacg tcaccggccg cctcggtgaa gaccttgccc atctcctgcg
     2641 tcatctcgcg ggcgagatcg ttcttgcgct cctcaaggat ccgtgccgtg cggtagagga
     2701 tctcgccccg tttcggcgcc ggcaccagcc gccagctcct gaacgccttg ctcgcggcgg
     2761 cgactgcctc atcgacgtcg ccggcattgc tcttcgtgaa cgtgccgacg acctcgccgg
     2821 tggcggggtt gcgccgctcg aaggtctctc cgctcctcga ctcgacccac tcaccgccaa
     2881 tgaagttctt ccagacttca gacacctgcg ctgcgtcctt tccgcgtctc catgtcgccg
     2941 tcgcactcga ccgcttcggt cctggttcct gatcagagcc gcggcaagtc taccaaagca
     3001 cctcggacag cgggcacgaa aaaagccgtg ccctgcagga cacggtcctg gctccccgag
     3061 taggattcga acctacaacc taccggttaa cagccgggcg ctctaccgtt gagctatcgg
     3121 ggatcacgtt cgcgatcgaa ccgttcgaga ttgtaccaag acgaccggcg gcgacgcaag
     3181 gcgcgtatgc atacttggta gcgtgtatga gtcgatgccg cacacccggg acctgcctgc
     3241 atgtggaacc ccaacagcta ccgcgacagc gactatggtc tgtcgaacga cgaacgtttc
     3301 gagccaaccg acaagctcga tgacgacttc gcgccgcgtg agggcgagcc gttcatccag
     3361 acagtgctcg ggccgatcgc accagacgaa gccggcgtcg ctctggtgcg cgaatacctg
     3421 cagtggcgac caggtgggct ggcagacgtc caagcggcgc tgaccgacct tgaagccttc
     3481 ttcaccgcct caggtcgcac gatcgtttcc gccacggcgc ccgacgccgg acgggatgcc
     3541 cacgtgctcc tcacgctcgc ccaacacgcg cccctccaca tcatcggcgg caccggctgg
     3601 tcgatcgttc catcaagcga gttcaacagc cgctccatcg aagaggggat tgagcgcgac
     3661 atcaccgacg gaatggacga cacgccggct cgtccgggac tggtggtcgg gagtatcggg
     3721 gccgacatgc cgccgaacgt cgtcggcgat gccctcggcc tgctggccag cgcgcagcgc
     3781 cgtttcgggc tccccgtcat ggtggattgc ggcggacgcg acaacctcaa gcgcgtgctc
     3841 gagtcaaagc tcgacgtcga agggctcatc gtgacgggcg acgtcgtgcg gactcaggat
     3901 agcgccatac gcatagcaga aggcggcgca tttgtgctgg tgtcgccact cgaaggcatc
     3961 aacgccgagc gggatcggca ggctgcccgg ctggttaccg cgctcgtcga ccgtggatgg
     4021 cagcatcggg tcctggtgtc gcacgccttt acagatcgct ctaggctaaa tggctacggt
     4081 ggccgaccgg gcctgtcata tcttgtagag cagtttgccg ttatgctatt ggaaagcggg
     4141 atgcatgcac acgacgtgca gcagatcgtc atcgataacg cggctctcgc cctatcggtt
     4201 cgaggagcgc agcaatctgc ctgagcgggc tggaagcgga gggcccacgt gacgacccga
     4261 gtctatctcg ccgcggcgat gttccgcgag gagccaatgc agccgcgcga tctgccggcg
     4321 gagcgcgtct tcgtcaacgc gtcggacgtc aaggaggtct gggtcgagac cgagagcgac
     4381 gaccaagttc ccgatatcgg cagggccgtt gcgttcgcgc tcgtcaaccc gatcgaaatc
     4441 ggcttccgcc gcatcactgg aaccgtcgaa cgcaagatcg acaagacccg ccagaactga
     4501 acctcacaga gcggtctcca gcgcgatcgc accgggcagc gcgccagagg ccgggatagt
     4561 cgcccgacct ctcccgggtc ttccggcgcg cttctcagtc acgcgaaaag cgcggtatcc
     4621 gctgccgctt gtgctccgaa ctcgcgacca tctgcatcac gcgctcgacc gtcgctggat
     4681 cggcgtcgcg ggtgtcacca ctctcgatcc cctcaagcgt acggtcgagc tcatcgtagg
     4741 tgatgccgat ctcgtcctca tccgtctggc cctgccagag tcccgccgag ggaggccgct
     4801 cgatcacctg ctgcggcacc ccgatcgctc gcgcgacctc gcggacctca cgcttgtaga
     4861 gatcgacgat tggcagcagg tcgacgccgc catcaccgta tttggtgaag tatccgatca
     4921 tcgcctccgt cttgttgccg gtgcccagca cgatgccgcg gcacaggttc gccacgtagt
     4981 agagcgtcgc catgcgcaga cgcggcttga tgttcgcgtc ggccagctgc accgcctcgg
     5041 cgggtggtgc acttggcagg ccgagccgtt cctggacatc cggggcggac ggcaacaccg
     5101 cacgcaggct ttgggtcggc gcggtgaggt cgatcgtcgt cgccggaatg ccgaacgcct
     5161 gcgcgacgag tgctgcggac tcgaggtcgc tcggattgga cgccgcaggc atcatcacag
     5221 cgaagacccg atcgctgccg acggctcggg ccgcgaggcc agcgaccacc gcggagtcga
     5281 taccgccact gagaccaagg acgaagcgat ccgcgccgct ccactccagt tggtcgcgca
     5341 gccaagccga aatctgatcg gccagctcga tggcgagagt tcgctcgcca acgcttgttc
     5401 ttgtgtccat cagacccccc tccgcgagag cggggacagc tggcgggccg tccccgccgg
     5461 tgtactcaac tcgctagata tggatcgcgt ggcccagcgc gtcgtgcgcc gcctcgccga
     5521 tcacctcgat aaccgtcggg tgcgggtgga cagtccagcc gagctcctga acgctcatgt
     5581 tgaggagccg gccgagtgcc gcctcgccga taagctcggt ggcgtgtggt ccgacgatgt
     5641 gaacgcccaa cacgtcgtcc gtgtcggcat cgaccacgag cttggtcatg ccctccgtgt
     5701 ggccctcgat ggtggccttg gcgttcgggc gcatcgggaa ggaacctatc ttgacctcgt
     5761 agccgagctc cttcgcctgg tcctcggtga gacctaccga accgatctcc ggctcagaat
     5821 aggtgcatga tggggaattc agaatatcca gcggctcggg ggagagaccg gcgatgtgct
     5881 cgaccgcgat gatgccctgg tgttgcgcgg tgtgcgcgag catctgctga ccgttcacgt
     5941 cgccgatggc gtagacgccc tcgacgttgg tctgcgtcat ctcgccaaca ggaatgatgc
     6001 cgcgctcggt cttgacaccg accgtctcca ggccaatgtc ctcgatgttg ccctcgcggc
     6061 cgatggcgac gaagagggtg tccgcctcaa ccgactgctc cttgcccttg gcgtctttca
     6121 ccgtcacctt gacgaccttg tccgagatgt cgaagtcgtc agcggtggga cgggccccgg
     6181 gaatcacatc cagcttctgc cggcgaagag atcgctcgac ctgcgcgcga atatccttgt
     6241 cctcattggg gacaacattc ccgaccatgg tcaccttggc accatagcga tggtagatgc
     6301 tggcccattc gatgccggtg gcgccgccgc cgcggacgat cacgctcttc ggcatgtcct
     6361 cgcgcaccac ggcgtggtcg ctgttgaaga ccgtcttgcc gtcgaactcg atccccttga
     6421 tggcgcgcgg gcgggacccg gtgttgatga tgatcttgtt gccggaaagc ttgaaggctt
     6481 cgccgccctc cttcggcgta acctcgacgg tgttcttgtc gagcatcttg ccccgcccga
     6541 cgatgacggg gatcttgtgc ttcttgtcaa acaggtaggt caggccgcga tactggctat
     6601 ccacaacacc cagtgagcgc ttgagggcca cgtcgtagtt gaacgtcaca tcgcccttga
     6661 tgtcgatccc gaactcggcg gcgcgtttga cctcatcgaa gacgcctgcc gacttgagca
     6721 tggccttcgt cgggatgcag ccacgatgga ggcaggtgcc gcccaggatg agttcttcca
     6781 ccactgcgac actgagccca agctgcctcg cgcggatggc ggccacatag ccacccgtgc
     6841 cgccgcccag aagcacgagg tcgtatttct gatccctgtc ggagtcagcc atcgatcttc
     6901 cttctccaat catccgtgcc cccagcgcgc gcgacaggag gcggtcactc ggcgttcatt
     6961 atccacgatt gcacgttggc tcggcaggca cgcgcgtaaa gcttcgctgc ggcgtgtgcc
     7021 catggggaca cgccctgccg cttcagaaca gcgaaagcgc caggcgaccg atcgcctggc
     7081 gcttgcttct ggtcgtcgct tttgagctct tgtctgtgga acggccactc caacatgctc
     7141 ggtcgagcgt cttacgcggc gttccggttt tcctccggcg cgttggcctc ctcgatgaag
     7201 cctcgcaggg cgggcacgtc aacctcaagc gagttgcggc cgagggaccg aaccagattg
     7261 cgggactgca tatcgagcag cttgcgcgtg acggtgacgc ggttcgagcc gatcatgttg
     7321 gcaagcgcct gatgggtcgc ggaccgggtg acgcgcagca cgccttccga ctcggtctcc
     7381 tcaccgaccg cctttgcaag atccaccagg atcgtggcaa gcctcaccga cgtgtcgcgg
     7441 gtaagcacct gctcgatgaa gtcgtgcgtt gccgtctgcc ggctcgcgag cgcgctgagc
     7501 agcttgcgtg agtactcggg cgacttcgcc agcacggcgc cgacgcgctc gaacggaatc
     7561 tcggtcacct cgcaatcggt gagcgcatct gcagccgtcg tcggcgtcac gccgtcgctg
     7621 ccggtgtcct gcatgaaggc ctcacccggg ccgagcagac ccagcaccag cgtgcggtcg
     7681 tcgttcagta tgcggtagag ccgcacccag ccgtcgcgga tcaggaacag cgatccctcg
     7741 ttttcaccgg cgaacatcac ggtgtcgtcg gcagcgaact tgtggctcaa cacccgtgcc
     7801 tcggaacgaa cgggctgcag ggtttggttg gtacgtcgat ggtccatgaa acgatacatg
     7861 ccggctcctc tcgatgcccg atacgccgtt tcctgatggg atgcgtggca ccggacgatg
     7921 ttactacacg aaaatctcac gcgcttattg tagcgcagcg caccaagtgt gtcaaggttt
     7981 tgcacagctt ttgcagcgtg cttgacagga acgacaccgg cctttcagcc tgttgactgg
     8041 cccccctcgc agagcgttcg agccactccg tcccgaattc cactcgggat cgccagaatg
     8101 cgctgtggac tgcagcgcaa gaccagtgcc tcaacgatgg cgaagctggc gggcagcacc
     8161 tgcgcgcggt cgaactgtac cgggattcgc tccatcgtct cgatcgcatg gcgggagccc
     8221 agccagtcgg cgagcagctc gagacggtct ggcgcgagct cggcaccgga cgacaggctg
     8281 ccggtgagca aattgaggta gtgaccggtt ccgccgggca gcaggagcat ccgggcggag
     8341 tagggcacca gaccgctctt gcccagagcg gtcgaagcgc gctcccgggc tcgtgccatc
     8401 gagccatcgg gaggtggcga cgtgccgatc tcctcgtcga aaagcatccc ggatcccgtg
     8461 ggcaccgacc cagagctttc cagattgccg tcggcgcgca cagcgatgag ttcggtgctc
     8521 gcgccgccga tgtccgccac gacgttcacg accccaggct cgatccacgg tctggcgccc
     8581 tcaagggtca accgagtctc ctcgtcggcg tcgatcacat tgacacgcca gcgcgtctcc
     8641 cgctcgatcg acgcgacagc ctcctggctg ttggcggcaa cccgcaacgc ctgtgtcgca
     8701 acggccagga actgactggc accaagttcc accgcccggg tctcataccg ccgcaggacg
     8761 tcgagcagcc gctccagtcg ctcctcgctg atccggcccg tctcgctcac gcggtacccg
     8821 atccggacgg tgtgggcgtc gccgtagagc ggacgcagcc gccggcccgc gtcgcactcg
     8881 taaaccgtga ccttcaccgt attgctgccg atgtccacga cgccgaatct tgctagccgc
     8941 gcaaccgagg cgctcatatc gggcggttgt aacgcgcgga ctcgagccgc cggtacgcct
     9001 cttgccagtc aggcatgccc gatgtctcca tcaacccccg cgtttcctcc agtgggtagt
     9061 ccacgtagtg ccaggtcgga tcccagccac caccctcgac ggcctcgaaa acaacgtatc
     9121 gcggccgcag gtccccgtcc cgcgggttcc cgacggccga cacgtcgacg agtgtgcggt
     9181 cgccaacctg ccggacgtag ctgacgtgga gatggccgaa ggcgatagtc ttggcaagct
     9241 cttcgccgag aatctcgttg atttcccgat cgctcgcatt cggcgagaga tgtcgctcta
     9301 cgtccgttgg gttggcatgt acgacaagga gttcggaggc agggccggca ttgcccgccg
     9361 gatgtcgcac ccggatcgag aaagggaggt cgcgcaggaa ggcgaggccc ccggcaccga
     9421 tttgctcccg agtccaggcg atcgagccgc tggacgcagc tccggcgtcc agcatatcgc
     9481 ggtcggtgtt gccgtagaca caccgggcgt catgtaaccg gagcaattgc agcacgcgcc
     9541 tcgggtccgg cccgccctcg aagagatcgc ccgcaacgat cagctgctcg acatctcgat
     9601 gctcgagatc ggcgagcact gcctcgaagg caggggcgaa cccgtggata tctgacatga
     9661 cggcaatctt catgcgacgc tcctcccaac ggcccgttcg ccgtctactc gtcgttacgg
     9721 tacacgaacc aacgccaagt cggcactgga ggagttcatg cagcgatttc gtcaggaccg
     9781 cactcttgcg acgcccagct ccggggagat attcatcggc aaggtctttt cccagcagat
     9841 ggtgacggac cagaacgcgc cctcgctccg cgtcaattcg ctcacctttg aggaaggcgc
     9901 gcgaaaccgg tggcaccgcc actcaaccga gcaggtgctg attatcatgg agggtcgagg
     9961 aatcgtggag aacgagagcg agacgtttca ggtcgctgcc ggcgacatcg tcctgatccc
    10021 cgccggcgag cgacactggc acggcgccga agaaaatgcc tcgatgaccc atctctcgat
    10081 tctgatcccc ggagagctga cgattgacga cgaggggtga ccctcgtcgt catcgcacat
    10141 ccgttccttg ccgagatcag gagatgggcg tcgcggcggg cgtcgctggc gccgcttcgg
    10201 cagcgaggat cgtctcgagc atctccacgt tgggggccat tgcgtaggtg acctcgtcgc
    10261 catcgaggat gacgcgagtc acgacttcgt ctgtctcgaa ctgcagcacg ttgtccgcga
    10321 cgtaggttgg gcccacgccc cagcgctcgg tttcgtagag cgcccaggcg cgtgcgaact
    10381 cctcggcgtc ctgaggggtg tcccacgccg actcccagag gatggcgtcc tccgtgtccg
    10441 tgccagccac gaggtaggta tctccgcccc agccggcggc agcgcgctcg gcctgctcct
    10501 cgcccatcga cgtctcgccg aggatcacgc gcagttggta ctcgccgaac gtgttggtgt
    10561 caaagaccgt ccagtcgtcc ccaagctcct gggtaaagtc ggcaacctcg atttcgacag
    10621 gcgccccacc gtccaggtac ttctctggat gaagaatttg ctcggtgctc gccggagggt
    10681 ctgcgagcgc ctggttcacc agatcccagc cgccctcgct gtaaagcgct tccacgaagg
    10741 tcaacccatg ctcatacggg aagatcaacg tattggagat aatgaccggt gcagcgtcaa
    10801 gcaccgagct gtcgaactcg gttacctcaa tctcctgcaa aaacgcctcc agcaactccg
    10861 gattctccat caggtactgg agctcggcgt agctggcgtc gccctcgatg agcgcagtga
    10921 ccgcaagcgc ctggtcgtcg ctcagcggct cgcgatcgag gacgccggcg tcgaggtcga
    10981 agtgctggtc ctggagcgca tgcacaatct cgtgagcgta ggtgacctcc tgagagggag
    11041 tgaagcctgc ctcgtcggtc gcgtcgcgga tcacgaccat ctcgctcgtt tcgggatcgt
    11101 agtagcccgc gatctgctcg ctgtagaggt cgatgtagag gtccccgaag tcgatatgct
    11161 cgtccatcag gccgaaggca agcagctcgc gctcgtcggc atactgctcg tcctcgggat
    11221 agtcctcgag caggtcctcc tgcagttcga ccgccagctg ctcgcgcgtc atcggtgtga
    11281 gctcgatcgg ccgaagcggc tcgagaccgc gcacgtcggc gacaatctgc gtgatgtcct
    11341 caaactcctg cggctcagcc tcctgcgcgg agactggact cgcgagcgcg aagagtgcaa
    11401 ccatgacgac caagatgagc ggttgtcgct gtcgcttcac cgggtgactc ctgagtgagt
    11461 ggcgcaccgg cgccacggcg gaaatgaatg ggaacgacgt tcacatgacc cgtgaagtat
    11521 ttcatatctg agtcgccagt tcggcaactg gcaccactat ggttgcagga agagttcgag
    11581 agtggagagg tgggtatccg catggccgaa cgcacagtta cccggtaccg cgcactggcg
    11641 gagaccgtct tttcgcttcg gttcgaggcg atgccggtct ggatcgccag cgatctggtt
    11701 gcgtggcttg acgatcgctc cggcgtgatg cagctctacg ccggcaaccc gataacgggc
    11761 gagcgacgcc aactcacgac tggcgccgaa cgcatccagt cgctgatcgg caccgccggt
    11821 tca
//
LOCUS       DASYWL010000202         3041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_572536, whole genome shotgun sequence.
ACCESSION   DASYWL010000202 DASYWL010000000
VERSION     DASYWL010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572536"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VGR22_07090"
     CDS             <1..366
                     /locus_tag="VGR22_07090"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEV2128363.1"
                     /translation="DIATWDGIEAWLSSMVARLEDRGVSAGCPLGTIVAEVADRDDRF
                     QTLAAKAFSLWEAQLAEGLQALKDKGGLRGDADPETLAEETLASIQGGYLLAVAKREA
                     RPMKNATRAAFSRLQSYAG"
     gene            1319..2332
                     /locus_tag="VGR22_07095"
     CDS             1319..2332
                     /locus_tag="VGR22_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEV2128364.1"
                     /translation="MTTLVTGGAGFVGSHLCDRLIRTGEPVICLDNLVTGRLENIQGL
                     IGHPRFTFVQHDAIESLPALPSLRRIYHLASPASPPWYQAYPVETLRVNSEGTRHLLE
                     RASRDGARFLFASTSEVYGDPLVHPQPEGYRGNVSTTGPRSMYDEAKRYGEALTVACG
                     QSWGVETRIVRIFNTYGPRLDPRDGRVVSNFVCQALAGKPLTVYGDGSQTRSFQYVDD
                     LVEGMVRLMTSSCTDPVNIGNPGEVTMLELAHLVQELTGTTSPIVFERLPTDDPRQRR
                     PDICLARAELGWEPRVPLADGLEHTIGYFRRELWPSDGARHAEGTSHRASHAAVAMPA
                     PAR"
     gene            2344..>3041
                     /gene="galE"
                     /locus_tag="VGR22_07100"
     CDS             2344..>3041
                     /gene="galE"
                     /locus_tag="VGR22_07100"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV2128365.1"
                     /translation="MGTVPSDLGETSKAAPRVLLTGGAGYIGSHTAKALVARGLEPVI
                     LDALTTGAHEAARWGTFIHGDIGDRTLLRSVLRQHPVDGVIHFAASAYVGESVAHPRK
                     YFHNNVANMLALLDELLDAGVTQVVFSSSCATYGVPDTVTITEDHSQRPVNPYGESKL
                     FGERMLAAYGHAYGLRWVALRYFNAAGADPDGELGENHDPETHLIPLVIDAALGHRGP
                     LEVYGTDYPTADGTA"
BASE COUNT          552 a         1050 c          926 g          513 t
ORIGIN      
        1 gacatcgcga cctgggatgg tattgaagcc tggctgagct cgatggttgc ccggttggag
       61 gatcgcggcg tctccgccgg ttgtccgttg ggaacaatcg ttgccgaagt tgcggaccga
      121 gacgatcgat tccaaacgct cgccgccaaa gcgttctctt tgtgggaagc ccaacttgcc
      181 gagggactgc aagcgctcaa ggacaagggc gggctacggg gcgatgccga tccggagacg
      241 ctcgccgagg aaacgctcgc cagtatccag ggcgggtacc tcttggcagt tgccaagcgc
      301 gaggcacgac cgatgaagaa cgcaaccagg gcagcgttct cccgactgca gtcgtatgcg
      361 ggctgaccag gcgtggaggt atagcgcagt gtgtgggggt gtgcccgctc gatgacggct
      421 ttctcgaagc accactccag catcacctgg tgggcccatg gcttcatcga catgcggtac
      481 gtggtcggga agacctcgtg gagggtgtgc tcctcggggt tgccgaagtc gacgacctcg
      541 ccatccagtc cgtcctgctc catgaacagc accaagccgc gcaccaagtc ctcgacttct
      601 cgaatgggga aatcatctca agatattgta cttcggggaa gggttgctcc caatattcaa
      661 ttgcacctcc cccatcccca cggtggtcgc gacaggcgcc ggacattcgc cggagccggc
      721 gcctgtcctt gatccggtgt atctattccc ctcttctggc acaggctttg cgctctcctc
      781 ggtgatgacg ggcacgaccg catcaccgaa ggatggacac caccatgaac gacccacaac
      841 ccagagagga cgaggcgtac tcaagcacta tcctttaatt ctgcaatgcc gtcatcacca
      901 cgctcccggc caggacgcct cggccaatct ttgccgcaaa tacgtgcgtc atcattcctt
      961 tcccttctct caatggatcg gcttgcagca gcatagatgt cgaggcttgc cgtgcccgcc
     1021 gccggccgca cgtttcagat cttgacagta actgtaccaa gcggtacagt ctcaaatatt
     1081 catactaata ctaacgagaa gggtcgaggc gcaggtggag gcaagcatca agtactggct
     1141 ggcgggccca aacccgaaaa agatcgccga ggagacctcc gttactgatc atccgcgacg
     1201 aggcggcgaa cttcctccca atgaacacgt cagggtcacg gccgagcatc ggcagcgcac
     1261 cactaccgac gtctcgctat gctgacgtcc acatcgccaa caaacagggg aatggacaat
     1321 gacaacactc gtcacgggtg gggccgggtt cgtcggctcc cacctctgtg accgcctcat
     1381 ccgtaccggt gagccggtga tctgcttgga caacctggtg accggccggc tggagaacat
     1441 ccaggggctc atcggacacc cccgcttcac cttcgtgcag cacgacgcga tcgagtcgct
     1501 gccggctctc ccgtcgcttc gccgcatcta ccacctcgcc agcccggcga gcccgccgtg
     1561 gtaccaggcc tatccggtgg agacgctgcg cgtcaacagc gagggtaccc gccacctgct
     1621 cgagcgcgcc tcccgggacg gtgcgcgctt tctcttcgcc tccacctcgg aggtctatgg
     1681 tgacccgctc gtccaccccc aacccgaggg ctaccgcggc aacgtcagca cgaccgggcc
     1741 ccgctccatg tacgacgagg cgaagcgcta cggcgaggcc ctgaccgtcg cctgcggcca
     1801 gtcgtggggt gtcgagaccc gcatcgtccg catcttcaac acctacggcc ctcgactgga
     1861 tccgcgcgac ggccgggtgg tcagcaactt cgtgtgccaa gcgctggccg ggaagccgct
     1921 caccgtctac ggcgacggct cgcagacgcg cagcttccag tacgtcgacg acctcgtcga
     1981 gggcatggtg cggctgatga cgtcgtcgtg caccgatccg gtgaacatcg gcaacccggg
     2041 cgaggtcacg atgctcgagc ttgcccacct ggtccaggag ctgaccggca cgacgtcacc
     2101 catcgtcttc gagcgactgc cgaccgacga tccgcgccaa agacgtcctg acatctgcct
     2161 cgctcgcgcc gagctcgggt gggagccgcg cgtgccgctg gccgacggtc tggagcacac
     2221 catcggctac ttccgcagag agctgtggcc gagcgacggg gcacggcacg ccgagggcac
     2281 ctcccatcgc gcatctcacg ccgccgtcgc gatgcccgct cccgcgcgct agggagtcgt
     2341 gccgtgggga ccgttccatc ggatctggga gagacatcca aagcagcacc ccgtgtgctg
     2401 ctgaccgggg gcgccggcta catcggcagt cacaccgcca aggcgctggt cgcccggggg
     2461 ctcgagccgg tgatcctcga cgccctgacc accggcgccc acgaggccgc ccggtggggc
     2521 accttcatcc atggagacat cggcgaccgg acgctgctcc gctcggtgct gcggcagcat
     2581 cccgtcgacg gcgtgatcca cttcgccgcg tcggcctacg tcggggagtc ggtcgcccac
     2641 ccccgaaagt acttccacaa caacgtcgct aacatgctcg ccctgctcga cgagctgctc
     2701 gatgccggcg tcacgcaggt ggttttctcg tcatcctgcg cgacctacgg cgtgcccgat
     2761 accgtcacca tcacggagga ccactcccag cggccggtca acccatacgg tgaatccaag
     2821 ctgttcggcg agcggatgct cgcggcctac gggcacgcct acgggctgcg ctgggtggca
     2881 ctgcgctact tcaacgccgc cggcgccgat cccgacggtg aactcggcga gaaccacgac
     2941 ccggagacgc acctcatccc gctggtgatc gacgccgccc tcgggcaccg gggaccgctc
     3001 gaggtgtacg gcaccgacta cccgacggcg gacggcacgg c
//
LOCUS       DASYWL010000203         5778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_290752, whole genome shotgun sequence.
ACCESSION   DASYWL010000203 DASYWL010000000
VERSION     DASYWL010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290752"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(297..1484)
                     /locus_tag="VGR22_07105"
     CDS             complement(297..1484)
                     /locus_tag="VGR22_07105"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEV2128366.1"
                     /translation="MSAHAAVVATPDWKMAVDLLEAELGGPLTSPDVLVVFINDAWQS
                     RYPELLRELRQRTGASTIIGGSASGVLAEGREYEDMPGLSCLALWLPEVTATPVRLHQ
                     ESLPLLDDPETWHAHTGVDPAGLTGVLLVADPYRMDAHALLCGVRGCYPGVPVVGGMT
                     SASEHRRQTWLFFDEQVYDEGGVALLLDGPVRIQPVVAHGAEPVGEVWTVTKADRHCI
                     LEISGRPALDMLFETAKVVYGNEQQGGFSYGDWLIGFAANEYQDRFHRGDFIVRGILG
                     SDHDRRGIVVGGMPRVGQTVQFQLRDAALADVDFRQHLLDVRAARDAQPAAALLFTCN
                     GRGEDMFGEPDHDAATVQTLLPGVPVAGMFCNGEFGPASPKQTPVLHGFTATLGLLIP
                     AGS"
     gene            complement(1791..2792)
                     /gene="ispH"
                     /locus_tag="VGR22_07110"
     CDS             complement(1791..2792)
                     /gene="ispH"
                     /locus_tag="VGR22_07110"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEV2128367.1"
                     /translation="MSTVSAAAGTDTGTKKERRIVVADTLGYCWGVRRALDIIEEAAN
                     PESKVAPIGDIIHNPQVVERLRARGVEGQQDLDQVEREGFKRVAITAHGYGPQLTEAA
                     KTRGLEMIDTTCPLVTKVQRLAQKLVKQGYFLVVYGDDYHPEVRGVLNWANTSKSCAA
                     KKIEQLPWNAKRGETGEGKVSPPRKVALVSQTTKNLDELMKFAGKLQEMVVPLGGELR
                     FANTICEPTTERQEALKRLVDQADVILAIGGKKSSNTARLADVGNARGVPSYHIERPE
                     EIQSEWLEPAGTLGVTAGASTPDDVIEGVIAYLADTYGFTPPEGGIRPVDPDYVPSY"
     gene            complement(2815..3972)
                     /locus_tag="VGR22_07115"
     CDS             complement(2815..3972)
                     /locus_tag="VGR22_07115"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV2128368.1"
                     /translation="MTLGVVRRVVLSVFLLPLVVAALGSASAQHAVISAMSAAERAEL
                     EPNELGVIPVLQYHLFTTDPAEENEFTRTIEDFRADFQWLYDHSFYVIPMADLVRNQI
                     SAPPGKHPVVLSFDDATPGQFRLIEQEDGNLLVDPNSAVGVMEEFYRKYDDFGRGGFF
                     ALLHFNCFGGDIEPDQAQYCGRKLEWLAQNGYEIGHHTWDHQDLLDISDEEFQYQIGE
                     LWQWIEENIPAEGARPSMLVMPYGNYPDRDLHQEQRRMMREGVPYEGEVYVLEAAFMV
                     GAEPAPSPSSTEWDPIWIPRIQAFDEELEWWLPVMADGGVILYTSDGDPETVMVPDPL
                     PGWLGGALDPELTAESGKTLIQYDPETGELVGAAAIDNSQAWRRSAAIPAV"
     gene            complement(4184..>5778)
                     /locus_tag="VGR22_07120"
     CDS             complement(4184..>5778)
                     /locus_tag="VGR22_07120"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEV2128369.1"
                     /translation="FTYNGLTWTPTLAELGASIDVESSVDAAWELGRDESAVDRLGFT
                     RQLLRSDQRVPLRTTVDRSVLASWFTSVNADIDQRAVDASLEVNGADVMVIPERNGTI
                     VDEAAATEMVLGALESLEPLSAELPTQVEHPKIYADDLEGPRADLAAALDAPVLVMFE
                     GRKWDIPPADLAQFLTVETVYERGDTHVELSFDREALATYLRDAFAGQVNRAPVDASI
                     AWSVDQGGLVSLDQSVDGAALRSNAFADAVAESFLGDQSAVEIPVVVTKPEIDSSNLG
                     ALGIDGLLARGDSNYDGGKPDRDTNIEVGVKLLNGELVAPGEEFSFNRAVGEITYDKG
                     FVDAGVIEAETIGRDVGGGICQVSTTVFRAALLSGMPMTEWWPHSLRLKGYERDGWTA
                     GFDASILQSGSNPEYWGDFRFKNDTDGYMLVHAWTDYPHVIVEIYGNDDGRTVDISEP
                     KFWKPDGEYEDKEVIDPDKPVGYIEQTQWPTEPYAASFTRTITYADGTTVDRLFESIY
                     EGSGNVWRVSPDMKGKSPASRD"
BASE COUNT         1010 a         1936 c         1810 g         1022 t
ORIGIN      
        1 ctcaacccgc tcctcgatac cagcagcgcc agcacccgca ttgcggcatc cctgctggca
       61 acagcgctcg gcctgcgcat gctcccccca cgggattgag cgagccgcag gcgacgacgt
      121 ggctgcgccc cgcgatcccg ataccgtccg ccggcaacgc agccacgtcg tcccggcaca
      181 tggctgcttc agttgcagag agcgggagga ctcattcgct acgtccaaga tgacatggtt
      241 tttcgccaat tcccgaaagg gctgaaggcg acgcgcaagc cagcgcaagg cgtgacttac
      301 gaaccggcag ggatcagcag gccgagcgtg gcagtgaagc cgtggaggac cggagtttgc
      361 ttcgggctgg caggaccaaa ttctccattg cagaacatcc ccgccaccgg cacccccggc
      421 agcagggtct gcaccgtcgc ggcatcgtgg tccggctcgc cgaacatgtc ctcgccgcgc
      481 ccgttgcagg tgaagagcag ggccgccgcc ggctgagcat ctctcgcggc ccgaacatcc
      541 agcaggtgct gccggaagtc gacgtccgcg agcgccgcat cgcgcagctg gaactgcacc
      601 gtctgcccga cgcgcggcat gccgccgaca acgatccccc tccggtcatg gtcgctgccg
      661 agaatgcccc gcacgatgaa gtcacctcgg tggaagcgat cctgatactc gttggcggcg
      721 aacccgatca gccagtcacc ataggaaaac ccaccctgct gctcgttccc gtagacaacc
      781 ttcgcggtct caaacaacat gtcgagcgcc gggcggccgg agatctcgag gatgcagtgg
      841 cggtctgcct tcgtgaccgt ccacacctca ccgaccggct cggcaccgtg ggcgaccacc
      901 ggctggatcc tcaccggacc gtcaagcaac agtgcaaccc caccctcgtc gtagacctgc
      961 tcatcgaaga agagccatgt ctgtcgtcgg tgttcgctgg ccgaggtcat gcctccgacc
     1021 accggcacgc cgggatagca gccgcgcacg ccacagagga gcgcatgcgc gtccatgcgg
     1081 taggggtccg caacaaggag gacgcccgtg agccccgccg ggtcgacgcc ggtgtgcgcg
     1141 tgccaggtct ccgggtcgtc cagcagtggc agcgactcct gatgcagccg cacgggtgtc
     1201 gccgtgacct ccggcagcca gagcgcgagg caggaaagac cgggcatgtc ctcgtactcg
     1261 cggccctcgg cgagcacacc ggatgcactg cccccgatga tggtgctggc tccggtgcgc
     1321 tggcgcagct cgcgcagcaa ttcaggatag cgggattgcc aggcatcgtt gatgaagacg
     1381 accagcacgt ctggcgaggt caagggacca ccgagctcgg cctccagcag gtcaaccgcc
     1441 atcttccagt ccggtgtcgc gacaacggcc gcgtgggcgc tcacggacga cggcgggcca
     1501 tccgttcgcg aactggggcg gagcgtcgac tcatactgca tgcacattcc gatggtttct
     1561 ggcgtaggtc tgccttccgt gaccgggcga ggtgtccgga tacagcggct tgaccctgcc
     1621 gttcgatgcc gatgccggat tgtacccggg tatcggagaa acgcaacacg gcgtctccac
     1681 tcctgatcta ctgcagaccg ggcacagcga tgcagcgccc cggcaaatac agaagacggg
     1741 cggacatgga gtccgcccgt gcgccgttgt gcgtttgctt ctagatggaa ctagtacgag
     1801 gggacgtagt ccgggtcgac cggccggatg ccgccctctg gtggggtgaa gccgtaggtg
     1861 tcggcgaggt aggcgataac cccctcgatc acgtcgtccg gcgtcgaggc gcccgcggtc
     1921 acgccaagcg tcccggcggg ctccagccac tccgactgaa tctcctccgg ccgctcgatg
     1981 tggtacgacg gcacgccgcg cgcattgccg acgtcggcca gccgagcagt attggagctc
     2041 ttctttccgc cgatcgccag gatcacgtct gcctggtcga cgagtcgctt gagcgcctcc
     2101 tgtcgctcag tggtcggctc gcagatggtg ttggcgaaac gtaactcgcc gcccagcggc
     2161 accaccatct cctgcagctt gccggcgaac ttcatcagct cgtcgaggtt cttggtcgtc
     2221 tggctcacca gcgcgacctt gcgcggcggg ctgaccttgc cctcgcccgt ctcgccgcgc
     2281 ttcgcgttcc acggcagctg ctcgatcttc ttggccgcgc acgacttgct ggtgttcgcc
     2341 cagttgagca caccgcgaac ctcgggatgg tagtcgtctc catagacgac gaggaagtac
     2401 ccctgcttga caagcttctg cgcgagtcgc tgcaccttgg tgaccagcgg gcaggtggtg
     2461 tcgatcatct ccagcccgcg cgtcttggcg gcctcggtga gctggggacc gtagccgtgc
     2521 gcggtgatcg ccacccgctt gaacccctcg cgctctacct ggtcaaggtc ctgctggccc
     2581 tcgacgccgc gcgcgcgcag ccgctccacg acctgcgggt tgtggatgat atcgccgatg
     2641 ggcgcgacct tgctctccgg gttggccgct tcctcaatga tgtcgagtgc ccgacggacg
     2701 ccccagcagt agccgagcgt gtcggccacg acgatgcggc gctccttctt ggtaccggtg
     2761 tcggttccgg cggcagccga gactgttgac atggatgggt tcctgtccgt tgcgttatac
     2821 ggctgggatg gcggccgatc ggcgccatgc ctgggaattg tcaatcgcag cagccccgac
     2881 cagctcgccg gtctcggggt cgtactggat gagcgtcttg cccgactcgg cggtcagctc
     2941 cgggtcgagc gcgcctccga gccagccggg gagcggatcg ggcaccatga ccgtctccgg
     3001 atccccgtca ctcgtgtaaa gtataacgcc cccatctgcc ataaccggta accaccactc
     3061 cagttcctca tcgaacgcct gaatgcgcgg gatccagatg ggatcccact cggtgctcga
     3121 tggcgacggc gcgggctcgg cccccaccat gaaggcggcc tcgagcacat agacctcgcc
     3181 ttcgtacggg acaccctcgc gcatcatccg gcgctgctcc tggtgcagat cacggtccgg
     3241 ataattgccg tacggcatga ccagcattga aggacgggca ccttcggctg ggatgttctc
     3301 ctcgatccac tgccacagtt cgccaatctg gtactggaac tcctcgtcgc tgatgtcaag
     3361 cagatcctga tggtcccatg tatggtggcc gatttcgtag ccgttctgcg cgagccactc
     3421 cagcttgcga ccgcaatact gcgcctgatc cggctcgata tccccgccga agcaattgaa
     3481 atgcaggagc gcaaagaagc cccctcgccc gaagtcgtcg tacttccggt agaactcctc
     3541 catcaccccg actgcggagt ttgggtcgac cagcaggttg ccgtcctcct gctcgatcag
     3601 gcgaaactgg ccgggagtgg cgtcatcgaa gctcagcacg accgggtgtt tgcccggcgg
     3661 agcagagatc tggttgcgga cgagatcggc catcgggata acgtagaagc tgtggtcgta
     3721 gagccactgg aaatcagcgc ggaagtcctc gatcgtgcgc gtgaactcat tctcctcggc
     3781 gggatcggtg gtgaagaggt ggtactgcag caccgggatc acgccgagct cgttaggctc
     3841 cagttcggca cgctcggcag cactcatggc gctaatgacc gcatgctgcg cagaggcaga
     3901 tccgagagcg gcgacaacga gcgggagcag gaagacagag agcacaacgc gacgcacgac
     3961 acccagagtc atgtggcaga gtccttcttg agtaatgaga aaatggccag aggacgagag
     4021 cgtcccgttg aaatcctgca tggagtgctg tcgcggggca ggcgagcagc gatcggagcc
     4081 ataggtttat tccgatctat cccctgcaca tcccggagaa cgtcgccgac acacgatgtg
     4141 tcggcgacgt tcgtgtcaag gactcacttg attgaggggt cgatcagtca cgactcgcgg
     4201 gtgacttgcc cttcatgtct gggctcaccc tccagacatt gccactgccc tcgtagatgc
     4261 tctcgaagag gcggtcgacg gtggtgccgt cggcataggt gatggtccgt gtgaacgacg
     4321 cggcgtacgg ctccgtcggc cactgggttt gctcgatgta cccgaccggc ttgtcggggt
     4381 cgatcacttc cttgtcctcg tactccccat ccggcttcca gaacttcggc tcgctgatgt
     4441 ccaccgtgcg gccatcatcg ttgccgtaga tctcgacgat cacgtgcggg tagtccgtcc
     4501 aggcatgcac cagcatgtag ccatcggtgt cgttcttgaa ccggaagtca ccccagtact
     4561 ccggattcga gccggactgg agaatggagg catcgaaccc tgccgtccag ccgtcccgtt
     4621 cgtagccctt gaggcgcagg ctatgcggcc accactcggt catcggcatg cctgagagca
     4681 gcgcggcccg gaagaccgtg gtcgaaacct ggcagatacc gccgccgaca tcccgaccga
     4741 tcgtctcggc ctcgatcacg ccggcatcga cgaacccctt gtcgtaggtg atctcgccaa
     4801 ccgcccggtt gaacgagaac tcctcgcccg gggcgaccag ctcgccgttc agcagcttga
     4861 cgccgacctc gatgttggtg tcacgatccg gcttgccgcc atcgtagttg gagtcaccgc
     4921 gggcgagcag cccgtcgatt ccgagcgccc cgaggttgct ggagtcgatc tccggcttcg
     4981 tgaccacgac cgggatctcg accgcgctct ggtcaccgag gaagctctcg gcgacggcgt
     5041 cggcgaaggc attggagcga agcgcggcgc cgtcgacgct ctggtcgagc gagacgagcc
     5101 cgccctggtc aacgctccag gcaatgctcg catcgaccgg ggcccgattc acctgcccgg
     5161 cgaaggcatc gcgcaggtag gtcgcgagcg cctcccggtc gaagctcagc tcgacgtgcg
     5221 tatcgccccg ctcgtaaacc gtctcgactg tcaggaactg cgccagatcg gccggcggga
     5281 tatcccactt cctgccctcg aacatcacca ggaccggcgc atcgagcgcg gcggcaagat
     5341 cggcgcgtgg cccctcgaga tcgtcggcat agatcttcgg atgctcgacc tgcgtcggca
     5401 gctcggccga aagaggttcc agggattcca gcgcaccgag caccatctcg gtcgcggctg
     5461 cctcatcgac gatggtgccg ttccgctccg ggataaccat cacatcagcg ccattcacct
     5521 cgagcgacgc atccaccgcg cgctggtcga tatcggcgtt gaccgacgtg aaccaacttg
     5581 ccaacacgct gcgatcgacg gtggtgcgca gcggcacacg ctggtcactc cgcagcagct
     5641 gtcgggtgaa cccgagacga tcgactgcac tctcgtcgcg cccgagctcc caggcggcgt
     5701 cgaccgacga ctccacgtca atcgatgctc cgagctcggc cagcgtcgga gtccatgtca
     5761 gcccgttgta ggtgaagg
//
LOCUS       DASYWL010000204         1925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_553827, whole genome shotgun sequence.
ACCESSION   DASYWL010000204 DASYWL010000000
VERSION     DASYWL010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553827"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VGR22_07125"
     CDS             complement(<1..308)
                     /locus_tag="VGR22_07125"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEV2128370.1"
                     /translation="MQLKRDTAPALSLLSTLLIFGCAASETASQHDRQGAWRDLFDGA
                     TLDGWHNPYDWGEAWVENGEIRLRADRKFFLMTRESFRDFVFEGEVMLPDTASNSGFM
                     "
     gene            complement(332..1672)
                     /locus_tag="VGR22_07130"
     CDS             complement(332..1672)
                     /locus_tag="VGR22_07130"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2128371.1"
                     /translation="MEPNASSIDRRSFLKAASAAGVGLWASGGLSPAYGYRGSPNERL
                     RVAVMGVNGRGEVLAKSFATAQDSEVVYIVDVDTQVLAKVVGAVEELQEKKPEGVVDY
                     RRTLDDRNVDALVIAAPDHWHALATINGLKAGKHVYVEKPAAHNPAEGELMVAAQRKY
                     DRLVQMGNQQRSDPRSIEIVQQIREGLIGRPYYARTWYANARPSIGIGKPAAVPAHLD
                     WELWQGPAPRVPFHDNYVHYNWHWFWNWGTGEVANNGTHEIDLARWALGVDYPVRVTS
                     VGGRYHYQDDWQFYDTQDVGYDFADGKTIVWQGRSSNGFPVLNRGRGTSIHGTEGTVV
                     LDRDGYLVYDLKNNLVQDVSGQEKIDALDTRGGDVMTDRHIENFINAVRVSERLNAPI
                     HEGHVSSLLTHLGNIAQRTGDALRIDPKSGHIIGNAEAQKLWGREYAPGWAPVV"
     gene            complement(1717..>1925)
                     /locus_tag="VGR22_07135"
     CDS             complement(1717..>1925)
                     /locus_tag="VGR22_07135"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HEV2128372.1"
                     /translation="RGEHWLNDEKIVEYELGTPRMDSLVAVSKYSTVPGFADRRRGHI
                     VLQDHGDAVWFRNLRIRELSGNRR"
BASE COUNT          297 a          649 c          625 g          354 t
ORIGIN      
        1 aacatgaacc ccgagttcga ggcggtatcc ggcagcatca cctcgccctc gaagacgaag
       61 tcccgaaagc tctcgcgggt catgaggaag aacttccggt cggcccgcag ccggatctcg
      121 ccgttttcga cccatgcctc accccaatcg taggggttgt gccagccgtc cagcgtcgcc
      181 ccatcaaaga ggtctcgcca ggcaccttgc cggtcgtgct ggctggccgt ttcgctcgcg
      241 gcacagccga agatcagcaa ggtcgagagc agcgagagag cgggcgcggt gtcgcgcttg
      301 agttgcatga cgttgtgcgg ggttgcaggc tctacacgac cggcgcccag cccggtgcgt
      361 actcgcggcc ccagagcttc tgggcctcgg cattgccgat gatgtggccg ctcttcggat
      421 cgatcctcag cgcatcgccg gtgcgctggg cgatgttgcc gaggtgggtc agcagcgaac
      481 tcacgtgacc ctcgtggatc ggcgcgttga gccgctcgct gacgcgcacc gcattgatga
      541 agttctcgat gtggcggtcg gtcatgacat cgccgccgcg ggtgtcgagc gcgtcgatct
      601 tctcctgacc cgaaacgtcc tggaccaggt tgttcttcag gtcgtacacc aggtagccat
      661 cccggtcgag caccacggtc ccctcggtgc cgtgaatgct ggtcccccgg ccgcggttga
      721 ggactggaaa gccgttgctg ctgcgcccct gccagacgat ggtcttccca tccgcgaaat
      781 cgtagccgac gtcctgcgtg tcgtagaact gccagtcgtc ctggtaatgg tagcggccgc
      841 ccaccgaggt gacacggacg ggatagtcca cccccagcgc ccagcgggcg agatcgatct
      901 cgtgcgtccc gttgttggcg acctcaccgg tgccccagtt ccagaaccag tgccagttgt
      961 agtggacgta gttgtcgtgg aagggcaccc ggggcgccgg cccctgccag agctcccaat
     1021 ccaggtgagc cggcaccgca gccggcttgc cgatcccgat cgacggacgg gcattcgcgt
     1081 accaggtgcg cgcgtagtag gggcggccaa tcagcccctc ccggatctgc tggacgatct
     1141 cgatagaccg cggatcggag cgctgctggt tgcccatctg gacgagccgg tcgtacttcc
     1201 gctgggccgc caccatcagc tcgccctccg ccgggttgtg cgcggcgggc ttctccacgt
     1261 acacgtgctt gccggccttc aggccgttga tcgtggccag cgcgtgccag tggtccggcg
     1321 ccgcgatcac cagagcatcc acgttgcggt cgtcgagcgt gcggcggtag tcgaccactc
     1381 cctcaggctt cttctcctgc agctcctcga cagcgccgac taccttcgcg agcacctgcg
     1441 tgtccacgtc gacgatgtag acaacctcgg agtcttgagc ggtggcgaag ctcttggcca
     1501 gcacctcgcc ccggccattc actcccatca ccgcgacccg cagccgctcg ttgggtgagc
     1561 cgcggtagcc gtaggcgggg gagaggccgc cggaggccca cagcccgacc ccggcagccg
     1621 aggccgcttt gaggaaagag cggcgatcga tggaggatgc attcggttcc atgagacctt
     1681 gtggtgtggt ggatgcttgc ctgggcgcag gcttgcctag cgccgattcc cgctgagctc
     1741 gcgaatccgg aggttccgga accagaccgc gtctccgtgg tcctgcagga cgatgtgtcc
     1801 ccgccgccgg tcggcgaatc ccggaacggt gctgtacttg ctcaccgcga ccagagagtc
     1861 catgcgcggc gtgcccagct cgtactcgac gatcttctcg tcgttgagcc agtgctcgcc
     1921 acggt
//
LOCUS       DASYWL010000205        14494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_46844, whole genome shotgun sequence.
ACCESSION   DASYWL010000205 DASYWL010000000
VERSION     DASYWL010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 14494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14494
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46844"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..2483)
                     /locus_tag="VGR22_07140"
     CDS             complement(156..2483)
                     /locus_tag="VGR22_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128373.1"
                     /translation="MPLSSPRAVGFAVRGGMPVDAADWVTVDLGPFLEQRSRGLMIAT
                     EPVEPDGHGFEMARVTRELIISELRAMEDLPPAEAIGRAFATANGMLFDEGQTSPTRG
                     YDRKVLIGATAVVLEGHRCVVGHVPPGQVVFIEDALAYTVPDLQSWAPSFITSEEMPI
                     SPEPLGYTSWTAPILAETELSDGDEVFICSASLAESLASDLADTELRPQDLASYHGRS
                     PDAALDVFKGLLIADRIEDGAALVLAFPPQPGSLGTASMDDVRWRLRHRRRRARAQVR
                     ALLPRRREAAAVAALEGAEGDSVDEDTVQQEEPAYALRSPVRRMLSRRRRSADTWVTP
                     GEARQYGLPRTHGVQLHRSVSIDRGEPGWRAMLPRIPLAGPLLVALVLGLLVVLTFGS
                     WSLRSDEPADTTQLASLGEIDQYILAAAESSDPDDARQALTLAEQALFDAEEEGAPAP
                     ELAPRRAAITSSLDEIDNVLRLDNIARIGTLPDPLQGGGTSALLTGSGLFLVNGGLYQ
                     IRTDQQQVLSILEPGETAGGVEVGSLFDVAVDSVGLHVTDGDHVWTLQTDGSWAAVEL
                     GRINNLPWQPRPVGAFGGGIYLLQPEFRQIYRFDTEIEDGVAEPVDWVLASVAPELVR
                     AVDMTISRSIHVLLDNTNTGDEVLTYTRGDLDSREMVPYLDLETTRPVAVLDGSATQL
                     LYVAAEDEDGGVVVVFDPASDDAWQLRLPTDFSVDEADVAAPFEDLQDVAIDEDTGTL
                     YLVNDDAVWTAQYELPVDATSTPMPQPEGVQEEGG"
     gene            complement(2617..4752)
                     /locus_tag="VGR22_07145"
     CDS             complement(2617..4752)
                     /locus_tag="VGR22_07145"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2128374.1"
                     /translation="MSTWMERFAGKGRRGGNGVLVQIAREVVGRKRSLQGAISEVRHP
                     AVLDALSDEEFTVLSEIIEERVKSDREFATVLARLAHAAAHAKGFDRETVDAALALDA
                     LLPADDPARDREKLLRDAYRAAQRAGYVQGGRRALARLGQRAAQVGEPERARVLLQQQ
                     LDLGPDTSDTEDEVDAAIVLGDLLHRDGDTDGALELFQRAAGSAGKLRYAKGLSEALV
                     REIDLSRGRTSDQRLISLQERALGAARDTGDSALAAHLLIDLAEPKARGDDLAGAVKH
                     LEEALDLARDLGDMEMENQALEMLSVAERQLGRNEKAISRDADRVRIGTATGAPTAAR
                     DAIEHGAQLLELHRADEARQVLERALDMAEQRQDNELIQRAHGGVGIALTQLQRPAEA
                     LDHLATALEMARQNGEAKHEAQWLAAIGEALWHYDQPGEASKALQQGITVARREGEQG
                     IEAGILMLLGDIFGAQREFPEARAAFETALERFVARDDVESQIAALSSLGGLAVEARR
                     TEAAMRYYQQALQLAEESRDQVAAVRLYGRMARVAQRVGEPQAAIDALEQAVALADEL
                     DDVELLGTMYQRLAIAMDTAGHSDTLDTYEQALSLAREVDDVYGEAMMLVNIGSRLHN
                     EGEDQEAVEVLEHALALADQLGASGLHLRQRGEAVLAGIEQGSAPAPRSRGSRPLRPE
                     EPAPPHRDDAGYSTPIRAREYNGSAASSR"
     gene            complement(4749..5783)
                     /locus_tag="VGR22_07150"
     CDS             complement(4749..5783)
                     /locus_tag="VGR22_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEV2128375.1"
                     /translation="METTVTLERLQDESGTHALWDGDLPTLGDVFRARRVIARHLAPT
                     PLIEAEKLSDRIGVEAYVKCENLQPTGAFKVRGGLYLLSRLSEEQRARGVVGASTGNH
                     GQSIAYAARAYGAKATIFVPEVANELKVASMERLGATVVKKGADFNVAFEAARASAEE
                     QGAHFIHSANEPDLIAGVATASLEMLEEVPDLDAVFVPIGGGSGLCGATLVGKSLNPA
                     LQVIGVQSEEAPVVYESWRYRELKTLDRCDTFAEGIATRAAFSLPAAMLWNKVDEIML
                     VSDADLRRSILTLLETTAMLAEGAGAAALAGLYDRRHEFAGKKVGIILSGGNLTLESL
                     QEALTKERAW"
     gene            6051..6911
                     /gene="pyrF"
                     /locus_tag="VGR22_07155"
     CDS             6051..6911
                     /gene="pyrF"
                     /locus_tag="VGR22_07155"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEV2128376.1"
                     /translation="MSESHAPDTLSTFCARLEHRAADADSLLCIGLDPAIGWMPEGIS
                     DDADGVIEFCERLITATSDFVVAYKPNLAFFLAYGADGLDALYTVRDMIPAEIPVILD
                     CKIGDIGSTASAYATAWLDTLDVDAITVHPYLGEDSLEPFLSRTDKGVFVLAKTSNPG
                     SGDLQDKTLAETGEPLYRYVARRVASWADIYPATVGLVVGATWPEQLREIRGVAPDLP
                     ILLPGLGTQGGDLKASLLAGLLDNGTGVLCSASRGIIYASSGTDFAEVARSAAEQLRD
                     AVNAVRRQPA"
     gene            7034..8311
                     /locus_tag="VGR22_07160"
     CDS             7034..8311
                     /locus_tag="VGR22_07160"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEV2128377.1"
                     /translation="MTSVLQSAAHAPSRVVLAVLVVVLGIGFMTPVAHANITDGTGGA
                     SARQLGIAPELLPPDMGQTALAVLVPETGHTVSGTMLDYWRANGAASVYGNPISEPYL
                     APNGYYSQAFERGIFQFLPEVIWTDEPTVRLQPTGAAELAPHRDETRADGRRVAGDRR
                     VSKTVPASGNETRASEVYADGGRFSTETGFSISGQFGAWYDSHEGWFYLGAPISEPFR
                     QRGVMVQLFRNGMLMEQEGIVRPASLPAENPARYGVDQLAPVTADLPVYDESLFVQVF
                     NPYGIDTTGLPGRRSIVIDISEQKLTAYQGNQVILETLVSTGLAPNETEIGAFHVRMK
                     FPAQDMAGFTDSSGEVISVGSAEGEGRGESYSVEDVPNVLYINYDAEALHGAYWHNKF
                     GQRMSHGCINLPLDVAAFIYQWAPLGTAVTVIE"
     gene            8494..9330
                     /locus_tag="VGR22_07165"
     CDS             8494..9330
                     /locus_tag="VGR22_07165"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2128378.1"
                     /translation="MTSDTLMNTTVPKQDMLPTERPARVVAFVNQKGGVGKTTSTVNI
                     ASVLAGLGDNVLVVDIDPQGNATSSFGVEKQALDITLYDALMGDVDTTDAIIPGARER
                     IDLLPAIPMLAGAEVELVDQPRREYRLADAVAHIRSNYDVVLIDSPPSLGLLTVNALT
                     AADAVVIPVQCEYLALEGVSQVLATIDLVRRNLNPDLELIGVLMTMYDGRTRLSSHVV
                     DEVRRYFPDHLFSVVIPRSVRLAEAPSFGQVIGEYDPSSRGAAAYAEVARELRTRLAA
                     TR"
     gene            9369..10925
                     /gene="guaB"
                     /locus_tag="VGR22_07170"
     CDS             9369..10925
                     /gene="guaB"
                     /locus_tag="VGR22_07170"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEV2128379.1"
                     /translation="MSQKTIPDPTSLSAGISENGHHDGRLRPDKFTGIGLTFDDVLIL
                     PAHSDVLPATVSTETKLTDEISLKVPLLSAAMDTVTEARLAIALAREGGIGVIHRNLS
                     IEEQAAEVDKVKRSESGMIVEPVTLSPDHLVSDAEAVMARYHISGVPITDDENKLVGI
                     LTNRDLRFIDDNSQRIGDVMTKEGLITAPQGTSLDEASSILHRHKVEKLPVVDEAGYL
                     TGLITVKDIQKKIQYPNATKDDRGRLRVAAAVGVGQDAEERAQELVAKGVDLLVVDTA
                     HGHSQSVIETLRRFSKSLSVPILAGNIATGAAAQALADAGAAAVKVGVGPGSICTTRV
                     VSGTGVPQITAINNVAQVLDGTNVCVIADGGIQYSGDIAKAIAAGAHAVMLGSLLAGL
                     DESPGEVIIFKGERFKEYRGMGSLGAMRARSFSKDRYFQEDIGNVQKLVPEGIEGRVA
                     YKGPLNAMVYQLVGGLRSAMGYAGADSVDSLRTESQFVQVTAAGLRESHPHDITITQE
                     APNYRPGGLK"
     gene            11044..11448
                     /locus_tag="VGR22_07175"
     CDS             11044..11448
                     /locus_tag="VGR22_07175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03667 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HEV2128380.1"
                     /translation="MPLIDITTEFGQRVEHRLAGEQVIWLTTVRPDGTPQPSPVWFLR
                     GGDTLLIYSQPDKPKLRNIEANPKVALSFNATPSGGDVVVFSGESPPAREHAAYIEKY
                     QDDIQRLNSTPERFSDEYSVPIRVALTGLRGF"
     gene            11568..12371
                     /locus_tag="VGR22_07180"
     CDS             11568..12371
                     /locus_tag="VGR22_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2128381.1"
                     /translation="MARIIAFFNQKGGTAKTTSTLNVAAALAERGRRVLVLDLDPQAS
                     LTMALGVDVTALEMSVYDLLLYEQVGIANVVVPTSIRDIAIVPSHPDLAAAEMELLNV
                     LERERQLDNRLRQADLADYDFVLVDSPPALNIISINILIAIRELIIPIEPHPLSLMVL
                     QRLFDTLGRITRLNPKLEIVGFLPTKVHHSSRLAVDMLTTLETQFPELPLLPSIPLSV
                     RGAESTAEQTSVLHYMPRSSVAAAYRDVGSWLIDGRDVSVPTRGEASHA"
     gene            12364..13506
                     /locus_tag="VGR22_07185"
     CDS             12364..13506
                     /locus_tag="VGR22_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEV2128382.1"
                     /translation="MPERQQPDEFEDILTGRKTRRAPRQMPQDDTGEKSPHPGRSRRK
                     FTVDALFKDTRPQAVGVEDLAEAKEIALERIEPDPEQPRRSFDEERLEELTTSIRKEG
                     VLQPIVVRYDVERDRYIIIHGERRFRASTLAERRTIPALVRNVPESRRLVQQLMENIV
                     RDDLNAIDRARALRILKDQMDDAPWEDVAAEVGIKRSRLFQLLDTSKLPKPAQDHIRA
                     GTLSEKQSRALQGLPGPYQQALAEAIIERSVPSQVAARVGRAIRESKRPASSPVAVRA
                     HIDELLDLAISGSDDERATQVDRLLESLGAAAREEVDRASLAEIATALGAPKFTPARI
                     TNSVGDLAQSLAHADRDRIAKDVALRQRLRALGDIIGYLTAEDSQH"
     gene            14123..>14494
                     /locus_tag="VGR22_07190"
     CDS             14123..>14494
                     /locus_tag="VGR22_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128383.1"
                     /translation="MPHLKPARRHIRLLALALAGATLLAGCRLDLNADPEPTATPTAV
                     SFGPNDMPVGTLLNEASVAWDEVDAWVEESRVETQSVAAGGPPASVTTDRVILPDQRH
                     TLTTNGDTLVSEEIVIGDTIYM"
BASE COUNT         2632 a         4980 c         4425 g         2457 t
ORIGIN      
        1 cgtcgcctac ttcgagtaga aggtgagaac gacaacggga agcggccctc ggacatacac
       61 ggggttgctt ttcgcttcct tcgccggcaa ccgggctgaa tgacgcaata cgcgttctcc
      121 tgatccgagc gatcctcgca cggagccggc tcgactcatc ctccctcctc ctgaaccccc
      181 tcgggttgcg gcatcggagt gctggtcgcg tcaaccggca gttcgtactg cgccgtccag
      241 accgcgtcgt cgttcaccag atagagcgtg ccggtgtcct cgtcgattgc gacatcctgc
      301 agatcctcga acggcgcagc cacgtccgcc tcgtccaccg agaagtcagt cggcagccgc
      361 agctgccacg catcgtccga ggcagggtcg aagaccacga ccacgccgcc gtcctcgtcc
      421 tcggcagcaa cgtacagcag ctgtgtggca gagccgtcca gcactgccac tggtcgcgtc
      481 gtttccaagt cgagatacgg caccatctca cgtgagtcga ggtcgcctcg cgtgtaggtc
      541 aacacctcat ccccggtatt ggtgttgtcc agtagcacat ggatgctgcg gctgatcgtc
      601 atgtcgactg cgcgcacgag ctccggtgcc accgacgcga gcacccagtc gacaggttcg
      661 gcgacgccat cctcgatctc cgtgtcgaac ctgtagatct gacggaactc cggctgcagc
      721 aggtaaatcc cgccaccgaa ggcccctacc ggcctcggct gccatggaag attgttgatc
      781 ctgccaagct cgaccgcggc ccacgaaccg tcggtctgca gcgtccacac gtggtcacca
      841 tcggtaacat gaagcccgac gctgtctaca gcgacatcga agagcgagcc gacctcgacc
      901 ccgccggcgg tctcgccggg ctcgagaatc gagaggacct gctgctggtc ggtccggatc
      961 tggtagagcc ccccattcac gaggaaaagt ccactgccgg tcagcagggc actggtgcca
     1021 ccaccctgca gcggatccgg gagggtgccg atacgcgcaa tgttgtccag ccgcagcaca
     1081 ttgtcgatct cgtccagaga actggtgatt gcggcccggc gtggagcaag ctccggggcc
     1141 ggcgcccctt cctcttctgc atcgaacaac gcctgctcgg cgagtgttag cgcctggcgc
     1201 gcatcatccg gatcgcttga ttcagccgcg gcgagaatgt actggtcgat ctcgcccaga
     1261 gaggcgagtt gggtcgtgtc agcgggctcg tcggaacgca atgaccagct gccgaaggtc
     1321 agcaccacca gcagtccgag cacaagcgcc acgaggagcg gccccgccag cgggatgcgg
     1381 ggcagcatcg cgcgccagcc cggctcgccc cggtcaatgg agacggaccg gtgcagttgc
     1441 acgccgtgag tgcgtggcag gccgtactgc cgtgcctcgc cgggcgtcac ccaagtgtcg
     1501 gcggagcgtc ggcgtcttga caacatccgg cggaccggag aacgcagcgc gtaggcaggc
     1561 tcctcttgct ggaccgtatc ctcgtcgaca ctatccccct cggccccctc cagtgcggca
     1621 accgcggctg cctcgcggcg acgtggcagg agggctcgca cctgtgcccg cgcgcgccgt
     1681 cggcgatgcc gtagccgcca ccgcacgtcg tccatcgacg cagtaccaag agatcctggc
     1741 tgcggaggaa aggccagcac cagggcggcg ccgtcctcaa ttcggtcggc aatcagtagc
     1801 cccttgaaga catcaagcgc cgcatcaggc gagcggccgt gatagctggc gagatcctgt
     1861 gggcgcagct cggtgtcggc aaggtcagac gccagtgact ccgccaacga cgccgagcag
     1921 atgaacacct catcgccgtc ggacagctcg gtttctgcga gaatcggagc ggtccacgag
     1981 gtatacccga gcggctcagg agagatgggc atctcctctg aagtgatgaa gctcggcgcc
     2041 cacgactgca gatctggcac cgtgtaggcg agcgcgtctt cgataaacac gacctggccg
     2101 ggtggaacgt gcccaacgac acaccggtgt ccctctagga ccaccgccgt tgcaccgatg
     2161 agcaccttgc ggtcgtagcc acgggtggga ctagtctgcc cctcgtcgaa cagcatcccg
     2221 tttgcggtgg cgaaggcccg accaatggcc tcggcgggcg ggagatcttc catcgcgcga
     2281 agctcggaga tgatgagctc ccttgtgacc cgcgccatct cgaagccatg tccatctggc
     2341 tcgacgggct cagttgcgat catcaggccc cgcgaccgct gctcaagaaa tgggccgagg
     2401 tcgacggtca cccaatcggc ggcatcgact ggcataccgc cacgaacggc gaatccgaca
     2461 gcgcggggcg atgaaagggg catggcgggc atctcaacga acgatacaag tcggttcagc
     2521 catccgagtg taaaccggac tgtccgttca cgccgccagg aagtgtgacg cccggccgtt
     2581 acgggccggg cgcttcactg cttcgcaacg tgacggctat cgcgaagacg ccgccgagcc
     2641 gttgtactcc cgcgcgcgaa ttggcgtgga gtagccggcg tcatctctgt gaggcggagc
     2701 gggctcttca ggccggagtg gacgtgaccc acggctgcga ggcgcagggg cacttccttg
     2761 ctcgatgccg gccaacaccg cctcgccgcg ctgacgaagg tgcaggccgc tcgcgccgag
     2821 ctgatcggcc agcgccaagg catgctcgag tacctcgact gcctcctggt cctcgccctc
     2881 attgtggagc cgtgatccga tgttgacgag catcatcgcc tcgccataga cgtcgtcgac
     2941 ctcccgagcc aggctcagcg cctgctcgta ggtgtcgagg gtgtcggaat gaccagcggt
     3001 atccatcgcg atcgcgaggc gctgatacat ggtgccgagc agctctacgt catcgagctc
     3061 gtcggcaagc gccaccgcct gctccagtgc gtcgatcgcc gcctgcggct cgccaacccg
     3121 ctgtgcgacc cgcgccatgc gaccatagag acgcaccgcc gcaacctggt cgcgactctc
     3181 ctcagcgagc tgcagcgctt gctggtagta ccgcatcgct gcctcggttc gccgcgcctc
     3241 gacggcgagg ccaccgagcg aggacaaggc cgcgatctgg ctttcgacat catcccgggc
     3301 gacgaatcgc tccagcgccg tctcgaatgc ggcccgcgcc tcggggaact cccgctgcgc
     3361 accgaagata tcaccgagca gcatgaggat acccgcctcg attccctgct cgccctccct
     3421 gcgagccacc gtgataccct gctgcaacgc cttgctggcc tcgccgggct ggtcgtagtg
     3481 ccaaagagcc tcaccgattg ccgcgagcca ctgcgcctcg tgcttcgcct ccccattttg
     3541 ccgagccatc tccagcgcgg tcgccaggtg gtcgagcgcc tcggcgggac gctgcagctg
     3601 ggtcagggcg atgccgacgc cgccgtgcgc tcgctggatg agctcgttgt cctgtcgctg
     3661 ctccgccata tcgagcgccc gctctaaaac ctgccgcgcc tcgtcggcgc gatggagctc
     3721 gagcagttgg gcgccatgct ctatggcgtc cctcgcggca gttggggcac ccgtcgcagt
     3781 accgatccgg acacggtctg cgtcgcgcga aatcgccttc tcgttccggc caagctgccg
     3841 ttcggcgacc gacagcatct ccaacgcctg attctccatc tccatgtcac cgaggtcgcg
     3901 cgccaggtcg agtgcctcct ccagatgctt gacggccccg gcgagatcgt cgccgcgcgc
     3961 cttgggctcc gccaagtcga tcagcaggtg cgccgcaaga gccgagtcac ccgtgtcccg
     4021 ggcagcgccc aacgcccgtt cctgcagaga gatcagccgc tggtcgctcg tccgcccgcg
     4081 gctcaggtcg atctcgcgaa caagcgcctc ggagagcccc ttggcgtagc gcagctttcc
     4141 ggcagagcca gccgcccgct ggaacaactc cagcgcgccg tcggtgtcac cgtcacgatg
     4201 caggagatcg ccaagcacga tggcagcgtc cacctcgtct tcggtatccg atgtatcggg
     4261 gccgagatca agttgctgct ggagcagcac gcgcgcgcgc tcgggctccc cgacctgcgc
     4321 cgcgcgttgg ccgagtcgag caagcgccct gcggccgccc tgcacataac ccgcccgctg
     4381 cgcagcccgg tacgcatctc tgaggagctt ttcacggtcg cgggccggat catcggcggg
     4441 aagcagcgcg tcgagtgcca gtgcggcatc gaccgtctca cggtcgaatc ccttggcgtg
     4501 ggccgccgca tgggccaaac gagccaggac ggtcgcgaac tcgcggtcgg acttcactcg
     4561 ctcctcgatg atctcgctga ggacggtgaa ctcctcgtca gagagcgcgt ccagcacggc
     4621 aggatggcgc acctcgctga tcgctccctg gagcgaccgc ttgcgcccga ccacctcgcg
     4681 ggcaatctgg acgagcacac cgtttccccc acggcgtccc ttgccggcaa atcgttccat
     4741 ccatgtgctc accacgcccg ctccttggtc agagcctcct gcaggctctc gagtgtcagg
     4801 ttgccgccgg agaggatgat ccccaccttc ttgccggcga actcatgccg tcggtcgtag
     4861 aggcccgcca acgccgccgc gccagcccct tcggccagca tggccgtcgt ctccaagagt
     4921 gtcaggatgc tgcgccgcag atcggcgtcc gaaaccagca tgatctcatc gaccttgttc
     4981 cacagcatgg cggccggcaa cgagaaggcg gcccgcgtgg caatcccctc ggcgaaggtg
     5041 tcgcagcgat caagcgtctt cagctcgcgg taccgccagg actcatagac cacgggcgcc
     5101 tcctccgact ggacaccgat cacctgcaag gccggattga ggctcttgcc cacaagcgtg
     5161 gcaccgcaga gaccgctgcc accgccaatc ggcacgaaaa ccgcgtccag gtccggcacc
     5221 tcctccagca tctcgaggga agcggtcgcg acccctgcga tcaggtccgg ctcgttcgcc
     5281 gaatggatga agtgggctcc ttgctcctcg gcagatgccc gggcggcctc gaaggcaacg
     5341 ttgaaatcgg cacccttctt gaccaccgtt gcgccgaggc gctccatgga ggcgaccttc
     5401 aactcgttgg ccacctcggg cacgaagatc gttgccttgg caccgtaggc tctagccgcg
     5461 taggcgatcg actggccatg gttgccggtc gaagcgccga cgacgccgcg cgcccgctgc
     5521 tcctcggaca gccggctaag caggtagagc ccgccacgca ccttgaatgc cccggtcggc
     5581 tgcaggttct cacattttac ataggcttcg acaccgattc ggtcgctcag cttttccgcc
     5641 tcaatgagtg gggtcggggc gagatgcctg gcaatcactc gtctcgcccg gaagacatcc
     5701 ccaagtgttg gcaaatcgcc gtcccaaagt gcgtgtgtgc ccgattcgtc ttgcagccgc
     5761 tcaagcgtca cagtcgtctc cacctctcac ccgcaccatc gggccgcctc atcgtcacct
     5821 gcaacggcga tactgttacg gatcacgctc ggaaacaccg ctatcacgcg gttgccaata
     5881 gtgtccgtac acccagtcta gcatcggggt tttcgccgta acagatgttc cctattgtca
     5941 cacacgatgc caggctcatg ttgacccgcg tctcggctga cacgctggtg ttgtcgcccg
     6001 gtatactgca ggaaggcaat atgccgcgga gcttcgccac gaggtggttc atgtccgagt
     6061 cacacgcgcc cgacacactt tccaccttct gcgctcgact cgagcaccgc gctgcggacg
     6121 ccgactcgct gctctgcatc ggcctcgacc cagccatcgg ttggatgccg gaggggatca
     6181 gcgacgatgc ggacggtgtc atcgagttct gcgaacggct aatcacagcg acctcagact
     6241 tcgtggtggc ctacaagccc aacctcgcct tctttctcgc ctatggcgcg gatggcctgg
     6301 acgccctcta cacggtccgg gacatgattc cggcggagat cccggtaatc cttgattgca
     6361 agatcggcga tattggctcg acggcatccg cctatgcgac cgcctggctc gacacgctcg
     6421 atgtcgacgc catcaccgta cacccctacc tcggcgagga cagtctggaa ccgttcctct
     6481 cccggacgga caagggcgtg ttcgtgctcg ccaagaccag taatcccggt agcggtgacc
     6541 tccaggacaa gacgctggcg gagaccgggg aaccgctcta ccgatacgtc gcccggcgcg
     6601 ttgcgtcatg ggcagatatc tacccggcta ccgtggggct ggtggtcggt gcaacttggc
     6661 cggagcagct gcgggagatt cgaggggtcg cacccgacct gccgatcctg ctgccggggc
     6721 tcggcaccca aggcggtgat ctcaaggcat cgctcctggc agggctgctg gacaacggca
     6781 ccggcgtgct ctgctcggcg tcacgcggca ttatatatgc ttcgtccggc accgacttcg
     6841 cggaagtcgc cagatccgcc gccgagcagc tccgagacgc ggtcaatgca gttcgtcgcc
     6901 agccggcgta gcgaaggccc atgtccggac acaagcgcga ctaggcctgg attctgtaaa
     6961 ggtggcatat agttcggcgg gcgacaaaga ggccgacagg cggttcacac cggaatcaag
     7021 gggaatgaga cgcatgacat cggtccttca gtcggcagca cacgccccga gccgcgtagt
     7081 cctcgcggtg ctcgtggtgg tccttggcat tgggttcatg acacccgtcg cacacgcgaa
     7141 catcaccgat ggcaccggtg gcgcgagcgc acgtcaacta ggcatcgctc ccgagctact
     7201 gccgcccgac atgggacaga cggcgttggc cgtcctcgtc ccggaaacgg gtcacacggt
     7261 cagcggcacg atgctcgact actggcgggc caacggtgcc gcgtccgtct atggcaatcc
     7321 catttcggag ccgtacctcg cgcccaacgg ctactacagc caagcgttcg agcgcggtat
     7381 cttccaattt ctgccggagg tcatctggac cgacgagccg accgtgcgtc tccagccgac
     7441 cggcgcggcc gaactcgccc cacaccgcga cgagacgcga gcagacggac ggcgcgtcgc
     7501 gggagaccgc cgtgtcagca agacggttcc cgcctccggc aacgagaccc gggcgagtga
     7561 ggtctacgcc gatggcggtc gattcagcac agaaaccggc ttcagcatct cggggcagtt
     7621 cggcgcgtgg tacgacagcc acgagggctg gttctacctc ggcgccccga tctcggagcc
     7681 gtttcgacag cgaggggtga tggtgcagct cttccgcaat gggatgctca tggagcagga
     7741 gggcatcgtt cgtccagcat cactccccgc cgagaaccca gcccggtatg gcgtcgacca
     7801 actggctccc gtgaccgccg atctgccggt gtatgacgaa agcctcttcg ttcaggtctt
     7861 caacccgtac ggcatcgaca cgaccggcct gccggggcgc cgctcgatcg tcattgacat
     7921 ctcggagcag aagctcaccg cctaccaagg caaccaggtc attctggaaa cgctcgtgag
     7981 tacgggcctg gcaccgaacg agaccgagat cggcgcgttc catgttcgca tgaagtttcc
     8041 cgcgcaggac atggccggct tcaccgactc cagcggcgag gtgatctcgg tcggcagtgc
     8101 cgagggcgaa ggtcgaggcg aaagctactc ggtcgaggac gtacccaacg tgctctacat
     8161 caactatgac gcggaggcgc tgcacggcgc gtactggcac aacaagttcg ggcagcgaat
     8221 gagccacggg tgcatcaacc tgccactcga tgtggcagcg ttcatctacc agtgggcacc
     8281 gctcggcacc gccgtcaccg tgattgagta agtcgagtgc aaactcccgg gcgcgcaacc
     8341 accgcaccac gcgcgaacac accgcctttg agaggcgcca gaccagcatg ctacgatacc
     8401 gcaaccgaca ggagcctcca ggttcgttgc cggttgccca cccgccaatc agcggcggac
     8461 cgctgctgat tccagcccca tgacaggcca gccttgacct ctgacaccct gatgaatacc
     8521 accgttccca agcaggatat gctccccact gagcgtcctg cgcgggttgt ggcattcgtc
     8581 aatcagaagg gtggcgtcgg caagaccaca agcaccgtga acatcgcttc cgtgcttgcc
     8641 ggcctgggcg acaatgtact cgtcgtcgac atagatcctc agggcaacgc gacctcctca
     8701 ttcggggtgg agaagcaggc tctcgacatc acgctttacg atgcgctgat gggcgacgtc
     8761 gacaccaccg atgccatcat ccctggcgcg cgcgagcgaa tcgacctgct gccggcaatt
     8821 ccgatgctgg ccggcgcgga ggtcgaactg gtcgaccaac cacgccggga gtatcgactc
     8881 gccgatgccg ttgcccacat ccgctcgaac tacgacgttg tgctgatcga cagccctccc
     8941 tcgctcgggc tgctgacggt caacgccctc accgccgccg acgccgtggt gatccccgta
     9001 cagtgcgagt acctcgcgct cgagggagtg agccaggtac tggccacgat cgatctcgtt
     9061 cgccgcaacc tcaatcccga tcttgagctc atcggcgtgc ttatgacgat gtacgatggg
     9121 cgtacccggc tttcgagtca tgtggtcgac gaggttcggc gctacttccc cgaccatctc
     9181 ttctctgtcg tcatcccccg ttctgtgcgt cttgccgaag caccgagctt cgggcaggtc
     9241 atcggcgaat acgatccatc ctccagagga gcagccgcct acgccgaggt cgcccgggag
     9301 ctccgcactc gtctcgcagc caccagatga tccgtcgctg cctacggcac acatgtcagg
     9361 agttttgcat gagtcagaag acgatccccg accccaccag cctctcagcc ggcatctcgg
     9421 agaatggtca ccacgacggc cgcctgcgac ccgacaagtt cacgggcatc ggcctgacct
     9481 ttgatgacgt gctcatcctt cccgcgcact ccgatgtgct gccggccacc gtttcgacag
     9541 agacgaaact caccgacgag atcagcctga aggtcccgct cctctcggca gcgatggata
     9601 cggtcaccga ggcgcggctg gcgattgctc ttgcccgaga aggcggcatc ggtgtaatcc
     9661 accgaaacct ctccattgaa gagcaggccg ccgaggtcga caaggtcaag cgctcggaat
     9721 cgggcatgat cgtggagcct gtcaccctct cgcccgacca tttggtgagc gacgccgagg
     9781 cggttatggc gcgctaccac atcagcggcg tgccgatcac tgacgatgag aacaagctcg
     9841 tcggcatcct caccaaccgc gatctccgct tcattgacga caacagccag cgcatcggcg
     9901 acgtgatgac gaaggaggga ctcatcaccg caccgcaggg caccagcctc gacgaggcaa
     9961 gctccatcct ccatcggcac aaagtcgaga agctgccggt ggtcgatgaa gccggctacc
    10021 tgaccggcct catcacggtg aaggacatac aaaagaagat ccagtatccg aatgcgacca
    10081 aggacgatcg cggacgcctg cgggttgccg cagccgtcgg cgtcggccag gacgccgagg
    10141 agcgtgcgca ggaactggtc gccaagggcg tcgacctgct ggtcgtcgac accgcgcacg
    10201 gccactcgca atccgtcatc gagaccttgc gcaggttcag caagtcactc tcggtaccga
    10261 tcctcgccgg caacatcgcc acgggcgccg ccgcccaggc gctggccgat gccggcgctg
    10321 ctgccgtgaa ggtcggtgtc ggaccgggca gcatctgcac cacccgcgtc gtctcgggca
    10381 caggcgtgcc gcagatcacc gccatcaata acgtcgcgca ggtgcttgac gggacgaacg
    10441 tctgcgtcat tgccgacggt ggcattcagt attccgggga catcgccaag gcgatcgccg
    10501 ccggcgcgca cgccgtgatg ctcggtagcc tgctcgccgg actcgacgag agccccggcg
    10561 aagtaatcat cttcaagggc gagcgcttca aggagtaccg agggatgggg tcgctcgggg
    10621 cgatgcgggc acggtcgttc tcaaaggacc gatacttcca ggaagacatc ggcaacgtgc
    10681 aaaagctcgt gccggaaggg atcgagggac gggtcgccta caagggcccg ctcaacgcga
    10741 tggtctatca gctcgtgggc gggctccgct cggcaatggg ctacgctggg gctgacagcg
    10801 ttgattccct gcgcaccgag agccagtttg tgcaggtaac ggccgccggg ctgcgggaga
    10861 gccacccgca cgacatcacg atcacccagg aagcgcccaa ctaccggccc ggtggtctca
    10921 agtagtacac tcgcccattc gcgatgaaaa tcattgtgtc gccattccga ggtatcgaca
    10981 tggagccggc cgtacgggcg gctccatgtc cgccctgcca acaagcgtca ggagatccgg
    11041 ccaatgccac tcattgacat taccaccgag ttcgggcagc gcgtggagca ccgcctcgca
    11101 ggcgagcagg tgatctggct aaccaccgtt cggccagatg gcacgccgca gcccagcccg
    11161 gtctggtttc tgcgcggggg tgacacgctg ctcatctact cacagccaga caagccgaag
    11221 ctgcggaaca tcgaggcgaa cccgaaggtt gccctctcat tcaacgccac gccctcaggt
    11281 ggcgacgtgg tggtcttctc cggtgagtcg ccaccggcac gggagcatgc ggcctacatc
    11341 gagaagtatc aggacgacat tcagcggctc aactcgacac cggaacgttt ctcggacgag
    11401 tactccgtgc caattcgcgt tgcgctcacg gggctgcgag gtttttgatc ctcggcatca
    11461 tcccgacgcc ccggctggtg cctgttcctg ctacccttgt gtaccaccgt cggcttggct
    11521 gaaccggccg gattccgaac ccgcacccgc aggatggcac tctcgtaatg gcccgaatca
    11581 tcgcgttttt caaccagaag ggcggcaccg ccaagacgac cagcactctg aacgtcgccg
    11641 ccgccctcgc cgagcgcggc cgccgcgtgc tggtcctcga tctcgacccg caggcaagcc
    11701 tcaccatggc actcggcgtc gacgtcacag cgctggaaat gtcggtgtac gacctgctgc
    11761 tctacgagca ggtgggcatt gccaacgtcg tggtgccgac ctcgatccgg gatatcgcca
    11821 tcgtcccgtc gcacccggac ctcgccgctg ccgaaatgga gctgctcaac gtgcttgagc
    11881 gcgagcgcca gctcgacaac cggctgcgac aggccgatct cgccgactac gacttcgtgc
    11941 tggtcgactc gccgccggcg ctgaacatca tctcgatcaa catcctcatc gccattcggg
    12001 agctgatcat cccgattgag ccgcacccgc tctctctcat ggtcctgcag cgactcttcg
    12061 acaccctcgg acgcatcacg cggcttaatc cgaagctgga gatcgtgggt ttcctgccga
    12121 cgaaggttca ccactcgtcc cggctcgcgg tcgacatgct caccacgctg gagacccagt
    12181 ttccggagtt gccgctgctg ccctccattc ccctctccgt gcggggggcg gagtccacgg
    12241 ccgaacagac gagcgtgctg cactatatgc cacgatcctc ggtggctgcg gcctaccgtg
    12301 atgttggctc atggctgatt gacggccggg acgtctcagt gccaacgcgc ggggaggcga
    12361 gccatgcctg aacgacaaca gcccgacgag ttcgaggata tcctgaccgg gcgcaaaacc
    12421 cgccgcgctc cccgccagat gccgcaggac gacaccggcg agaaatcacc tcatcccggc
    12481 cgctcgcggc gcaagtttac cgttgacgcg ctcttcaagg acacccggcc acaggcggtc
    12541 ggcgtcgagg acctcgccga agccaaggag atcgcgctcg aacgcatcga gccggacccc
    12601 gagcaaccgc gacgcagctt cgacgaggag cgactggagg aactgacgac cagcatccgc
    12661 aaggaaggcg tgctgcagcc gattgtggtg cggtacgacg ttgagcgcga ccgctacatc
    12721 atcatccacg gcgagcgccg cttccgtgcg tcaaccctcg cggagcgccg gacgatcccc
    12781 gcgctggtcc gcaacgtgcc cgagagccgg cggctggtcc agcagctgat ggagaacatc
    12841 gtccgcgacg acctcaacgc catcgaccgc gcccgggcgc ttcgcatcct caaggaccag
    12901 atggacgacg cgccatggga ggatgttgct gcagaggtcg gcatcaagcg atcgcgcctc
    12961 ttccagctcc tcgacaccag caagctgccg aagcccgcgc aggatcatat ccgtgccgga
    13021 acgctttccg agaagcagtc gcgcgcgctg cagggcctgc ccgggcctta ccagcaagcg
    13081 ctcgcggagg cgatcatcga gcggtccgtc ccgtcgcagg tggccgcgcg ggttggacgc
    13141 gccattcggg agtcgaagcg acctgcaagc tcgccggtag ccgtccgtgc ccacatcgat
    13201 gagctgctgg atcttgccat cagcggcagc gatgatgagc gggcgacgca ggtcgatcgg
    13261 ctgctggagt cgctcggggc tgcggcgcgg gaggaggtcg atcgcgcgag tctcgccgag
    13321 atcgcgacag cactcggcgc accaaagttc acgccggcgc ggataaccaa ttccgtcggc
    13381 gatctcgctc agagcttggc acacgccgat cgagaccgca tcgcaaagga cgtggccctc
    13441 cgtcagcgtc tccgcgccct cggagatatc atcggctacc ttaccgccga agacagccag
    13501 cactgaccaa ggtcctccga tgcgaccatc cttcggagtc ggctcctctg catcaaccct
    13561 accctgtggg caccaatccc ctggcctgtc agaaagccgg gggccacctc aactcttcgc
    13621 ctggttggtg agctaccacc agtttgcacc tgttcggtgt cgggaatcca cgccgaacag
    13681 cagcgcgagg agatccagaa cgatcagcac aatcaggagg acgagcaggt ccacggtctg
    13741 cctccgatcg atcatcggtc gctggtggtg aaccagagcc ggtccgtctc gcggcgcagc
    13801 ctgtcggccg aggcggctcg acgaagatcg gccgcagcgc gctcgatggg tgccgtcgat
    13861 ggtgctggcg gcggaagctc gcaccggctc ggtacgatca cattgactcg cataccggtg
    13921 tcacttcctc gcccccgaac catcaaacgc tgaacagtgt tcacagtgct tcgctccttc
    13981 cagatccggt gcggtctcgc ttactgcacc tcttgccaga aggacgctcc tcgcggtgcg
    14041 ccgtgagatg tctcagccgg ccgcttctgc tacgctcgac agcagttatc gatgcaggcc
    14101 tgaattcacc agagtcacgc tcatgcccca cctgaagccc gcacggcgcc atattcgatt
    14161 gcttgcactg gcgctggccg gagcgacgct gcttgccgga tgccggctcg atctcaatgc
    14221 cgatcccgaa cccacggcca cccccaccgc ggtcagcttt ggcccgaatg acatgcccgt
    14281 cggcacgctg ctcaatgaag ccagcgtggc gtgggacgag gtcgacgcct gggtggagga
    14341 aagccgcgtg gaaacccagt cggtcgccgc cggcggcccg cccgcaagcg tcacgacgga
    14401 ccgcgttatc ctgcccgacc aacgccatac gctcacgacc aacggtgaca ctctggtcag
    14461 cgaggagatc gtcatcggcg acacgatcta catg
//
LOCUS       DASYWL010000206        17806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_441180, whole genome shotgun sequence.
ACCESSION   DASYWL010000206 DASYWL010000000
VERSION     DASYWL010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 17806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17806
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441180"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VGR22_07195"
     CDS             complement(<1..863)
                     /locus_tag="VGR22_07195"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEV2128384.1"
                     /translation="MLLTVDVGNTNTLFGLYTSDSEAPPITLRASSRRDRMPDEWYAI
                     LEPGLRRNGIIPEQIDDIAISSVVPGVTEWVRQMGAAVLGAETVVVRSTTDLGITVAT
                     DSPAEIGTDRIVNSLAARERYGSPAIVIDFGTATNFDVVDKAGRYIGGALAPGLVVAL
                     EAMASRAARLFSVELDFPEHALGRNTVHAMQSGLMFGYLSLIDGMIERLSAEIEGDPR
                     VISTGGLGASFAAHSPLIDHHDEHLTLLGLKLAWEHLRAEKREIGFVVEAERGGHQHL
                     PGAHGVVEVKHL"
     gene            921..2180
                     /locus_tag="VGR22_07200"
     CDS             921..2180
                     /locus_tag="VGR22_07200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEV2128385.1"
                     /translation="MAPSPGQQESYGAPPDRRHIPRWLIVLWSIGIAVLVFFGGIMVG
                     LLAERDRHEDAVVLSEDWQELGTVIEHLERDSYYRPGAGAEADAWREELERRAIEGLL
                     EGSGDNRAAFLPPAEAARSSAALTGEYEGIGVSIAENDQNEIEVVSVMLDSPAQRADV
                     RVGDVVETVEGTPIPAGELETASNLLRGGAGTDVEIALSRPGGEPYELTLTRERISTG
                     ETTVAYRFFPEEDLAIISISLVALTTTEELDTALEHAEEDGAGQLVLDLRGNPGGWVS
                     EATKVVGRFVDHEEGPALLEDTWPAGGGMIPLDIDGDGAERFDGEMLVLVDQHTASAS
                     EIVASALQYYDRAVVVGQPSFGKGSVQRVYDLDSGESLRLTVAEWFTPGEEPLEGIGV
                     TPDVELEDSTSSLEELVPTLDDVLGGE"
     gene            2440..3171
                     /locus_tag="VGR22_07205"
     CDS             2440..3171
                     /locus_tag="VGR22_07205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2128386.1"
                     /translation="MDRTEQPMVLLVEDDPTLRSTLAFNLRREGYMVLTADDGERAME
                     IVRDHEGQLDLLVLDVMLPRVNGFQILQRVRQRGIQLPVLMLSARGEEQDRIDGLELG
                     ADDYVVKPFALRELLARIRAALRRRAVPAARPPAVLQRGPLRIEPDRRRAAVNGAALH
                     LRPKEFSLLSTLALEPGKVFGRQELLNAIWGADVVVDERTIDVHISWLRGKLQDAGMD
                     GGCIRTVYGTGYCFHAALDAHSAVS"
     gene            3296..4297
                     /locus_tag="VGR22_07210"
     CDS             3296..4297
                     /locus_tag="VGR22_07210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV2128387.1"
                     /translation="MKFGNVFRRAVAVAVAGMFTLAQLVSAQQATEYAVPENIDDISG
                     AVATDGSSTVGPMTAAVAEEFRDVAENIDVTVDVSGTGGGFKRFCQGDIDISNASREI
                     KPEEVELCAENDVEFYEFEIALDALTVVVSSENHFVDCLTVEQLNQLWAPDSRITHWN
                     QLDESFPDEPIYLYGPGADSGTFDYFTEVINGEGGASRTDFQPSEDDNVIVQGVHYDP
                     YALGYLGLAYYLENATVLHAVAVDSGDGCVVPSAEAASSGDYAPLARPLYTYVRADSL
                     ERQAVQEFLRFYLANAEELADDVGYVPMSDEQIAEQWQKLEGAIEGSAAPDSEEELQ"
     gene            4484..5404
                     /gene="pstC"
                     /locus_tag="VGR22_07215"
     CDS             4484..5404
                     /gene="pstC"
                     /locus_tag="VGR22_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV2128388.1"
                     /translation="MAREPVDLSGKRNRNASEALIAAVLFLFAAFSLLVTLGIGGVLL
                     FEAVRFFREVPVTEFLFGNTWTALFLEKEWGVLPLVRGTLVVTLIALLVAVPLGLMSA
                     VYLSEYAGPRVRSILKPALELLAGVPTIVYGYFALTFITPSLLKPLFGREIHFQSALA
                     AGIAVGIMILPLMASLAEDTIRAVPYSLREGAYALGATKLETSLRVVVPAATSGIVAA
                     FILSMSRAVGETMIVALAAGARPADGWNPLEGMQTMTAYIVAVFTGDVVYGSLEFQSL
                     FAVGLLLFLMTLTMNILSQWIARRYRKVYQ"
     gene            5401..6312
                     /gene="pstA"
                     /locus_tag="VGR22_07220"
     CDS             5401..6312
                     /gene="pstA"
                     /locus_tag="VGR22_07220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV2128389.1"
                     /translation="MSLTTNLGTAAGSVRRHTWRPRIANRNRRARVLSFVFMLAAVSA
                     VLLLALLLGVLLLRGWDWIRLDLFTEQYSRNAEDAGLQAAILGSILLVAVSGVMAFII
                     GVGTALFMEEYAPKGRITDVLQTNISNLAGVPSVVYGLLGLALFVEMLNLGRVLLAGA
                     LTMGLLILPIVIIASQEAIRNVPLGLRQAAFGVGATRWQVIHHHVLPAAMPGILTGTI
                     LAISRAIGETAPLIVVGASAVVFANPDSFFDQYTAMPMQIYQWAKQPQEEFTQLAAAA
                     IIVLMVILLTMNGLAIYLRERLGKDRW"
     gene            6318..7160
                     /gene="pstB"
                     /locus_tag="VGR22_07225"
     CDS             6318..7160
                     /gene="pstB"
                     /locus_tag="VGR22_07225"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004060638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEV2128390.1"
                     /translation="MATEIPQTPLRAASVTQFKEHVDPEEHERVITISDLNVYYGDFH
                     AVAGVDIDIPENRITAFIGPSGCGKSTVLRCINRMNDLVPTARVEGSITYHGTEINSP
                     KVDPVEVRRRIGMVFQKPNPFPKSIYENVAWGARINGYRGDIDEHVERTLRQAALWDE
                     VKDKLKDSGMALSGGQQQRLCIARALATEPDVILLDEPASALDPISTLKIEELLRELQ
                     QNYTIVIVTHNMQQASRVSDRTVFFSLNERRQGVLVECGPTTKIFTNPEDRRTEAYIT
                     GRFG"
     gene            7211..7921
                     /gene="phoU"
                     /locus_tag="VGR22_07230"
     CDS             7211..7921
                     /gene="phoU"
                     /locus_tag="VGR22_07230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV2128391.1"
                     /translation="MTMRAAYTSQIQELMDVVVAMASMVDKAIARSIEALRTQNVQLA
                     QQVRKDDQLINDRRWAAEEQAITVIATQAPMAGDLRRIAACMQLYTDLERMGDHASGT
                     AKIVIETADEDLLKPLVDIPRMAEIAREMLGDAVTAFIEQDEDAAWKVAARDEEIDKL
                     YEQIYRELLTYMMADPATINRATHLLWAAHNVERLGDRVTNICERTVFTITGRFEEMD
                     GLPQRAVSQDSSANGTSG"
     gene            complement(7888..8358)
                     /locus_tag="VGR22_07235"
     CDS             complement(7888..8358)
                     /locus_tag="VGR22_07235"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2128392.1"
                     /translation="MSTLRPREDARRQHERPHVLIVTDDPSLIDFLGEGLVMGGFWTS
                     VISHGLQALEVFRHRQFDLLVIDRDLGTFDAVELMERLRGLSERGDIQPRSRAPVVVM
                     ASGDADLDVEQQERLGIATILQAPVELEDVVQTLHRVFVAWRDAHPDVPLADES"
     gene            8607..9146
                     /locus_tag="VGR22_07240"
     CDS             8607..9146
                     /locus_tag="VGR22_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128393.1"
                     /translation="MTVERLPDLVRELVERYLNRLRLPADQLMITADRREFERWLGRR
                     MSSSLGGAYIFLRHHDRHAILINLSRIDLDQPRALEVVVAEELVHMRDWIDGERRRHA
                     KHGYDRIAYRVAELTGASLEEVRSALLPLQRRPYRYVYACPRCGMRVKRRRRGRWSCG
                     RCAPRFDGRFELRIIEELE"
     gene            9212..9925
                     /locus_tag="VGR22_07245"
     CDS             9212..9925
                     /locus_tag="VGR22_07245"
                     /inference="COORDINATES: protein motif:HMM:NF016726.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEV2128394.1"
                     /translation="MRFIRLFLRGLKDAYDQFVLVIVVSALWWLSAILIVPGPPATVA
                     LFRMIDPRNQIQTPDIREFFRVIRENFRTGWGIAAFTVPVILVLLWNAAFFQGSDSFF
                     ALMVPLWLVMAFIVVMLMLYAFATAAAMDAKVRNAFRGATYLLVMWPFSSALMMILLT
                     LLGSLFAVLVLPLIFFGPGVSAAVINRFALTGFNVEIIDPEAPTSERRTEVAKGLNPD
                     DGGWLRRLRGGQHRQEGRR"
     gene            9922..10578
                     /locus_tag="VGR22_07250"
     CDS             9922..10578
                     /locus_tag="VGR22_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEV2128395.1"
                     /translation="MTRAIDRESPESLPEHGRKTSKWPRTTRRQERIQHVLARRQTSL
                     TVVLEDVHDPHNASAVLRSCDGVGILDVHLVYMQHEPPKRSFARTTSGSAAKWIRTHY
                     HDSIQACYRLLREQEMCICATALGRESDDLYALDLTQPTALVFGNEMRGVSEEAIAGA
                     DKMVFIPMQGMVESLNISVACAVTLYEAMRQRIAAGAYESPELSAEQLASLEQEWLRK
                     "
     gene            complement(10652..11383)
                     /locus_tag="VGR22_07255"
     CDS             complement(10652..11383)
                     /locus_tag="VGR22_07255"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEV2128396.1"
                     /translation="MSSPSSPDNHARHTSLGRATPAISAAMEDYLKAIHRLQQNEGRV
                     TTQALATELGLSAASVTNMVKRLAEVSLLEHSRYYGVRLTKAGAKVALEVIRHHRLLE
                     LYLAEAMGYDWDKVHEEAERLEHHVSSEFESRMDELLGHPEYDPHGDPIPSLHGELPS
                     AEWHALTEESAGSRVILRRVSDRDAKDLRFLNTIGLTPGVTFQVLDRAGDTGSDVEVR
                     LGGGRHAIPERLAAGMQIERVDGDQ"
     gene            complement(11439..12779)
                     /gene="ffh"
                     /locus_tag="VGR22_07260"
     CDS             complement(11439..12779)
                     /gene="ffh"
                     /locus_tag="VGR22_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEV2128397.1"
                     /translation="MFETLSDRLNDTFAKLGRKGRLTEKDVDEAMRDVRRALLEADVN
                     FKVVKQFVASVKERALGQDVMQSLTPAQTAIGIVNEELIKILGEERVPLNTPDRPPQT
                     IMMVGLQGSGKTTHTGKLALHMRKQGRNPLMVAADVYRPAAVNQLQALGKQIDMPVYD
                     EGTESDPVDIARNAMRFAQERGFNPVIIDTAGRLQIDERLMQELVNIKERVTPSDILL
                     VADAMTGQEAVGVAEAFHDQLDVTGLVLTKMDGDARGGAALSIRAVTGVPIKFIGTGE
                     KLDALEPFYPDRLASRILGMGDVLSLIERAQEQTTEEDAERLQDRMMKGQFDLEDFLD
                     QLQKIKSMGPLDQLLDMIPGLGAQMRQARTQVSDDDYKRIEAIIQSMTPWERRHPERI
                     SNSRRRRIARGAGRDRQEVNQMLSQFKEMQKMMGQFGQMAKKGKMPKNLPFNFP"
     gene            complement(12783..13700)
                     /gene="ftsY"
                     /locus_tag="VGR22_07265"
     CDS             complement(12783..13700)
                     /gene="ftsY"
                     /locus_tag="VGR22_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEV2128398.1"
                     /translation="MEQGLEKTRKGVFREITKLFDRAELDDELFEDLEMLLIQADVGW
                     DVSQQLVQELQDRIQEDHITNPADAREILRLEMIKLLELATQNRKIKLLQRGVPFVIL
                     VAGVNGTGKTTSIAKLAQYHKRFGRSVMLAAGDTFRAAAIDQIKVWGERVDVPVVAHQ
                     QGADPGAVVFDSVQSAQQRGVDVLIIDTAGRLQTKHNLMAELQKLRKIIQKQIPEAPQ
                     EVILVLDATTGQNGLVQAAEFTKSVEITDLMITKLDGTAKGGIAFSVAKEIGTPIAYI
                     GTGERATDLAEFNPETYVDSLFFGEGEGF"
     gene            13953..15209
                     /locus_tag="VGR22_07270"
     CDS             13953..15209
                     /locus_tag="VGR22_07270"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2128399.1"
                     /translation="MDRRELLLAVYLPTVLLAFTQGVIVLSLPLLANETSDAYSFASI
                     IVSAAAFGTLIADVPTGTVMLRLGLRRTMLIGSLLVALSTWALAIPLDAPAVLALRLA
                     AGIGTALWGLSRYTYITQKIPPELRGRTIAGFGGINRIGVFAGPMIAGSLVTAYGLYS
                     AFLLAGTLAAFAFAAAWRWIPGDAVAPRPPDAAHHAPDTGEIRGTWNAIAGNRSDVLF
                     GALAQVLAQVVRQGRHFLIPLYGVEVLSLEAVEVGLVMTASAVIDMVMFGPAGFLMDR
                     FGRKFATIPSFSVMAVGIAMIPFAGGFTGLLAAGIVIGFGNGLGSGTMMTLGADFAPS
                     AYTSTFLSLWRFIGDSGQMIGPILVGVVAQLFTLRQSAWVLSGCSVTCALMLFFLVRE
                     TRREHGSLEATEEGTEPEPVLQKQGG"
     gene            15213..16085
                     /locus_tag="VGR22_07275"
     CDS             15213..16085
                     /locus_tag="VGR22_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-opening dioxygenase subunit LigB"
                     /protein_id="HEV2128400.1"
                     /translation="MPLVAAAIAPHGFPVIPALGDDVEGEGETRPALAAMGEFFRQQG
                     VEAVVIAGPHGIRADGFFAVVDAARAAGTLHWKGRQVELNAPCDRPLIEAISMQASDD
                     GLPVARVSFAGNRADQAVVPLDWGALVPLWFLGHDQNEANTGNVLADPPSAPGGPPAV
                     LITPSRSLDRKAMVAFGRSIASAIERDERGVGFIASCDWAHTHREDGPYGFHEAADRV
                     DAIVVNAIRRNALMELIELPPEDVEHAAIDGLWQTLMLAGIQEVTPFDLQFRSYEAPT
                     YYGMIVATTAVGHD"
     gene            16078..16803
                     /gene="radC"
                     /locus_tag="VGR22_07280"
     CDS             16078..16803
                     /gene="radC"
                     /locus_tag="VGR22_07280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEV2128401.1"
                     /translation="MTEIARDRPPVYSLMVREMLPDERPRERLRMFGEGNLNTSELQA
                     IILNTGMKGESVISMTTRLLHDQGGLHGLYRTSFDELASTRGIGESKACKIKAALELG
                     RRLSAAAPAERVAIEGPDDVVRLVGVEMAALEQEQLRVVLLDTRHHVIRHRTIYTGSV
                     NSAQVRPGELFRDAVRANATAVIFAHNRPSGDPTPSVADIELTADVVKSGQLLDIAVL
                     DHVIIGQGRWVSMKRLGLGFAID"
     gene            16822..17625
                     /locus_tag="VGR22_07285"
     CDS             16822..17625
                     /locus_tag="VGR22_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV2128402.1"
                     /translation="MTLGFGVTSTIDQERVQRLAPAVEAAGFTFFWSNDIPGGSSFDV
                     LAAAAGVTETMSLANGVVPIDRVPPEAWQDRIREAGLAGDRFWPGLGSGRIHKPLEPM
                     GEAIETIQRTTGRPVVIGALRSRMRRLAAARADGVLLNWLTPAAAAVRREEMRDIRRE
                     AAVTRPFLAAAYIRVAIGDAALDRLRHEAEKYESFPSYAKHFAEMGAGAFETAVAATS
                     VEEASQALVPFIGALDHAVLRCVVGEETDDAYLQLVEAGRLAIERATQE"
BASE COUNT         3132 a         5503 c         5821 g         3350 t
ORIGIN      
        1 agatgcttga cctcaacgac gccatgggcg cctggaagat gctgatggcc gccgcgctca
       61 gcttcgacaa cgaaaccgat ctctcttttc tcggcgcgta ggtgttccca ggcaagcttg
      121 agaccgagca aggtgagatg ctcgtcgtga tggtcgatca gcggcgagtg tgccgcgaag
      181 ctcgcgccga gcccgccggt ggagatgacc ctgggatcgc cctcgatctc ggccgagaga
      241 cgctcgatca tgccatcgat gagggagagg taaccgaaca tcaggcccga ctgcatcgcg
      301 tgcacggtgt tgcggccgag ggcatgctcg gggaagtcga gctccacgga gaagaggcgc
      361 gccgcgcggc tcgccatggc ttcgagcgca accaccaggc ccggcgccag cgctccgccg
      421 atgtaccgcc ccgccttgtc gacgacgtcg aagttcgtgg cggtgccgaa gtcgatcacg
      481 atcgctggtg agccgtatcg ttcgcgggcg gccagcgagt tgactatgcg gtcggtgccg
      541 atctctgcgg gagaatccgt ggcaacggtg atgccgagat cggtggtgga gcgaacaacg
      601 accgtctcgg cgcccaggac cgccgccccc atctgccgga cccactcagt aacacccggc
      661 acgacgctcg agatcgcgat gtcgtcgatc tgctcgggaa tgatgccatt gcgacgaagg
      721 cctggctcaa ggatggcgta ccattcgtcg ggcatacggt cccgccgaga agaggcgcgc
      781 aacgttatgg gcggggcctc gctgtcgctc gtgtagagcc cgaatagcgt gttggtgttg
      841 cctacatcga ccgtcaacaa cattgcccac cattccccgt gagttcaccc gccagcgctt
      901 ccgtgcagag gcaattgttc atggccccat cacccggaca acaggaatca tacggcgcgc
      961 cacccgaccg gcgacacatt ccccgctggc tgatcgtcct ctggagcatc ggtatcgccg
     1021 tgctggtctt cttcggcggc attatggtgg ggctgctcgc tgagcgcgat cgtcacgagg
     1081 atgccgtagt actgagcgag gactggcagg agctgggcac tgtcatcgaa cacctggagc
     1141 gcgactccta ctaccgtccg ggtgcagggg cggaggcaga cgcctggcgg gaggagctcg
     1201 aacgccgggc gatcgagggc ttgttggagg gctcggggga caaccgcgcc gccttcctgc
     1261 cgccggccga ggcagcacgc tcctccgcgg cgctgaccgg ggaatacgag ggaatcgggg
     1321 tctctatcgc cgagaacgac cagaacgaga tcgaggttgt ttcggtgatg cttgactcgc
     1381 cggcgcagag agccgacgta cgtgtcgggg acgttgtcga aacggtcgaa ggcacgccga
     1441 tccccgcagg cgagctcgag accgcgtcca atctgttgcg tgggggtgcc ggaaccgacg
     1501 tggagatcgc gttgtcccgt ccgggcggcg agccgtacga gctaacgctc actcgcgagc
     1561 gcatctcgac aggcgagaca accgtcgcct accggttttt cccagaggag gacttggcga
     1621 tcatcagtat ttcgctcgtc gcgctgacga ccaccgagga gctggatact gcgctcgagc
     1681 acgcggaaga ggacggcgcc ggccagctgg tgctcgacct gcggggcaac ccgggtgggt
     1741 gggtgtcgga ggcgaccaag gttgtcgggc gtttcgtcga ccacgaagaa ggtcccgcgc
     1801 tcttggagga tacgtggccg gcagggggcg ggatgatccc gctcgacatc gatggcgacg
     1861 gagcggagcg gtttgacggc gagatgcttg tgcttgtcga ccaacacacc gccagcgcat
     1921 cggagatcgt ggcgagtgcg ctgcagtact acgatcgagc tgtggtcgtg ggccagccaa
     1981 gcttcggcaa gggatcggtg cagcgcgtct acgatctgga ttccggtgag tcactgcgac
     2041 taacggttgc ggagtggttc acgcccggtg aggagccgct ggaaggcatc ggggtaacgc
     2101 cagacgtcga acttgaagat tcgacgtctt cgctcgaaga gcttgttcca acgcttgacg
     2161 acgtcctcgg tggcgaatag acggaatcct ccgctacagt tcagccggtc ggactcgcaa
     2221 cccgccgacc tctgccgatc ggcagcccat actcactgca ttgctcctgc gctcatgggt
     2281 ataacatagc gcgatccctt cccctacgat ttttcgagga ttttacaaac cgattacttc
     2341 ccatttacgt gagccgcgta gcatacgaat cggtggcgat cgggtcagtc ggttgccacg
     2401 gaacgctatc cgacaccaac cagaaaggtg cggcatcacg tggaccggac cgagcagcca
     2461 atggtgctcc tcgtcgaaga cgatccgacc cttcgcagca cgctcgcctt caaccttcgc
     2521 cgagagggct acatggtact cacggcggac gacggcgaac gggcgatgga gatcgttcgt
     2581 gaccatgagg ggcagttgga cctgctggtg cttgacgtga tgctcccgcg tgtgaacggg
     2641 ttccagattc tgcaacgcgt tcggcagcgc ggcattcagc ttccggtgct gatgctttcc
     2701 gcccgaggtg aggagcaaga ccggatcgat ggcctcgagc tcggcgccga cgactacgtc
     2761 gtcaagccgt tcgcgttgcg cgagctgctc gcccgaattc gggcggccct ccggcggcgc
     2821 gcagtgccag ccgcgcgacc accggcggtg ctgcagcgcg gcccgctgag gatcgagcca
     2881 gaccggcgcc gcgcagcggt gaacggggcg gccctgcacc tccggccgaa ggagtttagc
     2941 ctgctctcga cgctcgcgct cgagccgggc aaagtcttcg gtcgacagga gctgctgaat
     3001 gcgatctggg gcgcggacgt cgtggtcgac gaacgaacga tcgatgtcca catctcgtgg
     3061 ctgcgcggga agctccagga tgcggggatg gatggcggat gcatccggac ggtgtatggc
     3121 accggatact gctttcatgc cgctctcgat gcgcactctg cggtgtcgtg atcgttctcg
     3181 tcttttcatc aggcgagtgg ctctgtaggg acggcgtctg agcctgtcgc agcgcgagcg
     3241 atgcgtagcg atggtcgctc gcccggggca gcacaccttg tttgagagga tcctgatgaa
     3301 atttggaaac gttttccggc gtgccgtagc ggtggcagtg gccggcatgt tcacactggc
     3361 ccagctggtc tcggcacagc aagccaccga gtacgcggtg cctgagaaca tcgacgacat
     3421 tagcggtgcc gtcgcgacgg acggctcatc gaccgtcggc cccatgactg ctgcggttgc
     3481 cgaagagttt cgtgacgtgg ctgagaacat cgacgtgacc gtagacgtct ccggcaccgg
     3541 cggcggcttc aagcgcttct gccaaggtga cattgatatc tccaatgctt ctcgggagat
     3601 caagccggaa gaggtcgagc tgtgtgccga aaatgacgtc gaattctacg agttcgagat
     3661 tgccctggac gcgctgaccg tcgtcgtgtc gtcggagaat cacttcgttg actgcctcac
     3721 cgtcgagcag ctcaaccagc tctgggcccc ggacagccgg atcacgcact ggaaccagct
     3781 cgacgagtcg tttcccgacg agccgatcta cctctacggc cctggggccg attcgggcac
     3841 gttcgattac ttcaccgagg tgatcaacgg tgagggtggg gcgagcagaa ccgacttcca
     3901 gccgagcgaa gacgataacg tgattgtcca gggtgtccac tacgatccgt acgcgctcgg
     3961 atatctcggc ctggcctact acctcgaaaa cgcgaccgtg ctccatgctg tcgccgtcga
     4021 cagcggcgat gggtgcgtgg taccgagcgc cgaggcggcc tcgtcgggcg actacgcgcc
     4081 gttggcacgc ccgctctaca cctacgtccg cgctgactcg cttgagcggc aggcagttca
     4141 ggagtttctc cgcttctacc tggccaatgc cgaggagctg gccgatgacg tcgggtacgt
     4201 cccgatgagc gacgagcaga tcgccgagca gtggcagaag ctcgaggggg ctatcgaggg
     4261 ctcggccgcg ccggacagcg aggaagagct gcagtgagac tgggaacggt aacgagctcg
     4321 gtctcactgc cgggcccatg gtgcattccg tatgcgccct acatcgccgc tattccggag
     4381 accgtctgcc ggcgaggatc ccatcccggt ttggttgcgt gcacggctgt cgagggggag
     4441 gattgaaaaa ttggctgttc aacgcgtacc aacgcggtcc gtaatggcgc gcgagccggt
     4501 cgatctctca ggcaagcgaa atcgcaacgc cagcgaggcc ctcatcgcgg ccgttctgtt
     4561 tctcttcgcg gcgttctcgc tgctggtcac actcggcatc gggggcgtgc tgctcttcga
     4621 ggccgtcagg ttcttccgcg aggtgcccgt taccgagttt ctgttcggca acacctggac
     4681 ggcgctcttc cttgagaagg agtggggcgt gctgccgctg gtacgcggca cgctggtcgt
     4741 cacactcatc gctctgctcg tggcggtgcc gctcggcctg atgagcgcgg tgtacctcag
     4801 cgagtatgcc ggcccgcgcg tccgctcgat cctgaagccg gcgctcgagt tgctcgcggg
     4861 cgtgcccacg atcgtctacg gatacttcgc cctgaccttc atcacgccga gcctgctcaa
     4921 gcccttgttc ggcagggaaa tccacttcca aagcgccttg gccgcaggca tcgcagtggg
     4981 catcatgatc ttgccgctga tggcctcact tgccgaggat accattcgcg ccgtgccgta
     5041 ttcgcttcgc gagggtgctt acgcccttgg cgcgaccaag ctggagacgt cactccgcgt
     5101 tgtcgtgccg gcagccacct cgggtatcgt cgccgcattc attctgtcga tgtcccgggc
     5161 ggtgggtgag acgatgatcg tcgcactcgc ggcaggtgca cggccggctg acggctggaa
     5221 cccgctggag ggtatgcaga cgatgaccgc ttacatcgtc gcggttttca cgggtgatgt
     5281 ggtctacggc agcctcgagt tccaatcgct cttcgcggtg ggcctgctgc tcttcctgat
     5341 gacccttacc atgaatatcc tgagccagtg gatagcgcgg cgctaccgga aggtgtacca
     5401 atgagcctga caacgaacct cggcaccgcg gccggaagcg tgcggcgaca tacctggcga
     5461 ccgcgcatcg cgaaccgcaa ccggcgagct cgagtgctct cattcgtctt catgctggcc
     5521 gctgtatcgg ccgtgttgct gctggcgctg ctgctcggcg tactgctcct ccgcggctgg
     5581 gactggatca ggctcgatct gttcaccgag cagtactcgc gcaatgccga ggacgccgga
     5641 ttgcaggcgg cgatccttgg atcgatcctg ctggtcgcgg tctctggcgt gatggcgttc
     5701 atcatcgggg tcggcaccgc gctcttcatg gaggagtacg cgccgaaggg acggatcacc
     5761 gacgtcctgc agaccaacat ctccaacctc gcgggtgtgc cgtccgtcgt ctacggactg
     5821 ctcgggctcg cgctcttcgt ggagatgctg aacctcggtc gcgtgctgct tgcaggagcg
     5881 ctcaccatgg gcctgctgat cctgccgatc gtcatcatcg cgtcacagga ggcgatacgc
     5941 aatgtgccgc tcggtttgcg gcaagctgcc ttcggggtcg gggcgacccg ttggcaggtg
     6001 attcaccatc acgtgcttcc ggcggcgatg ccgggaattc tcaccggcac catcttggcg
     6061 atctcccgtg ctattggcga aacggccccc ttgatcgtcg tgggcgcgtc ggcagtggtc
     6121 ttcgccaacc ccgacagctt ctttgaccag tacaccgcga tgccgatgca aatctatcaa
     6181 tgggccaagc agccgcagga agagttcacc cagttggctg cggcggcgat catcgtgctg
     6241 atggtgatcc tgctgacgat gaatggcttg gcgatttacc tccgcgagag acttggaaag
     6301 gatcggtggt agcaccgatg gcaacagaga ttccccagac cccacttcgt gccgcgtctg
     6361 tgacgcagtt caaggagcat gtcgacccgg aggagcacga gcgggtcatc accatcagcg
     6421 atctcaatgt ctactacggt gacttccacg cggtcgccgg cgtcgacatc gatatccctg
     6481 aaaatcgcat caccgccttc atcggaccgt cggggtgcgg caagagcacg gtgcttcgtt
     6541 gcatcaaccg gatgaatgac ctggtgccga cggcgcgggt cgagggcagc atcacctacc
     6601 acggcacgga gatcaactcg ccgaaggtcg atccggtcga ggttcggcgg cggatcggca
     6661 tggttttcca gaagccgaac cccttcccga agagtatcta cgagaacgtc gcctggggcg
     6721 ccaggatcaa cgggtaccgc ggtgacatcg acgagcacgt cgaacgcacg ctgcggcagg
     6781 cggccctctg ggatgaggtc aaggacaagc tcaaggacag cggcatggcg ctctctggcg
     6841 ggcagcagca gcgcctctgt atcgcccggg cgctcgcgac cgagccggac gtgatcctgc
     6901 tggacgagcc ggcctcggcg ctcgacccaa tctcgaccct gaagatcgag gagctgcttc
     6961 gggaactgca gcagaattac acgattgtca tcgtgacaca caacatgcag caggcctcgc
     7021 gcgtctccga caggacggtc ttcttctcgc tcaatgaacg acggcagggt gttctggtcg
     7081 aatgcggccc gacgacgaag atctttacca accctgagga cagacgcacg gaggcctaca
     7141 tcaccggtcg cttcgggtag aagcaccata atcgggaaaa tctgcagcga ataccatggg
     7201 agaatgctgg atgacgatgc gtgccgctta cacctcgcag atacaggagc tcatggacgt
     7261 cgtggttgcg atggccagca tggtggacaa agccatcgcc cgcagcatcg aggcgctcag
     7321 gacgcagaac gtccaattag cgcagcaggt gcggaaggac gaccagctga tcaatgaccg
     7381 tcggtgggcg gctgaagagc aggcgatcac cgttatcgcg acacaggccc ccatggccgg
     7441 cgacctgcga cggatcgccg cgtgcatgca gctctacacc gacctcgagc gcatgggtga
     7501 ccacgcgtcg ggcaccgcaa agatcgtgat cgaaactgcc gacgaggatt tgctcaaacc
     7561 gctcgtcgat atcccgcgga tggccgagat tgcgcgcgag atgctcggag atgcggtgac
     7621 ggccttcatc gagcaggacg aggacgccgc gtggaaggtt gccgcgcggg atgaggaaat
     7681 cgacaagctc tatgagcaga tctaccgcga gctgctgacc tacatgatgg ccgacccggc
     7741 cacgatcaac cgtgccacgc atctcctctg ggctgcgcac aatgttgagc gcctcggtga
     7801 ccgggtcacc aacatctgcg agcggaccgt cttcaccatc accgggcgct ttgaggagat
     7861 ggacggattg ccgcagcgag cggtgtctca ggattcgtcg gccaatggga cgtccgggtg
     7921 agcgtcgcgc caggcgacga acacgcggtg gagggtctgc acgacgtctt cgagctcaac
     7981 gggcgcctga aggattgtcg caattcctag gcgctcctgc tgttcgacat caagatctgc
     8041 gtcgccgctg gccatcacga ccaccggggc gcggctgcgt ggctggatgt caccgcgctc
     8101 cgacagacca cgaaggcgtt ccatgagctc aacggcgtcg aaggtgccga gatcgcgatc
     8161 gatgacgagc aggtcgaact gacggtggcg aaagacctcc agcgcctgca gtccatgact
     8221 gatgaccgac gtccagaaac cgcccatcac aaggccttcg ccgagaaagt cgatgagcga
     8281 tgggtcgtcc gtgacgatca ggacgtgtgg tctctcgtgc tgccggcggg catcttcgcg
     8341 gggtcggagg gtggacacgg cctatggctc ctggctgcgt aggcgaggat atgaaccaat
     8401 gggacagtca cgagcgtctg cgcacggaag agaggtcgga acgttaacga gcggcccttc
     8461 cctcgtcctg aacggactaa gagatgcggg tgagagcgcc gaccacttcg ctggattcag
     8521 tcttggcctg gtctcacgga tacaggctca ccaatactgt tcatgcgtct attgtgcacc
     8581 gataaaggga tacggggagg caatggatga ccgtcgagcg gttgcctgat ctggttcgcg
     8641 aactggtcga acgctatctg aaccggctcc ggctgcccgc cgatcaactc atgattaccg
     8701 cggacaggcg cgagttcgag cgctggcttg gccgtcgcat gtcgtcatcg ctcggcggcg
     8761 catacatctt cctgcgtcac catgatcggc acgcgatcct gatcaacctc tcacgcatcg
     8821 atctggacca gccacgggcg ctcgaggtcg tcgtcgcgga ggagttggtc cacatgcgcg
     8881 actggatcga cggtgagcgt cgacgccatg caaagcacgg atacgaccga attgcatacc
     8941 gtgtcgccga gctgaccggc gcgtcgctcg aggaggtgcg ctccgccctg ctgccactgc
     9001 agcggcgccc gtaccgctac gtctacgcct gtcctcggtg cggcatgcgg gtcaagcgtc
     9061 gacgccgggg gaggtggtcc tgcgggaggt gcgcgccacg gtttgacggt cggttcgagc
     9121 tgcgtatcat cgaggagttg gagtgaagcg ggtacactga cgggcggaag ccgctccggc
     9181 tgacacttga accgagatac aagagggaaa cgtgcgcttc attcgactct tcctccgcgg
     9241 cttgaaggac gcttacgacc aattcgtgct ggtgatcgtc gtctcggcgc tctggtggct
     9301 gtctgcgatc ctgattgtgc ccggacctcc ggcaacggtc gcgctctttc ggatgatcga
     9361 tccccgcaac cagatccaga cgccggacat ccgggagttc ttcagggtga tccgggagaa
     9421 cttccggacg gggtggggca tagccgcctt cacggtgccg gtcatcctcg tgctgctctg
     9481 gaatgccgcg ttcttccaag gctctgactc ctttttcgcg ctgatggtgc ctctctggct
     9541 ggtgatggcc ttcatcgtcg tgatgctgat gctgtatgcg tttgcgaccg cagccgcgat
     9601 ggatgcgaag gtccgcaacg ccttccgcgg cgcgacctac ctcctcgtca tgtggccctt
     9661 ctcctcggcg ctcatgatga tcctgctcac cttgctcggc agcctgtttg ccgtgctggt
     9721 gctgccgctg atctttttcg gacccggcgt ctccgccgcc gtgatcaacc gcttcgcgct
     9781 caccggcttc aatgttgaga tcatcgatcc cgaggcgccg acctccgagc gacggacgga
     9841 agttgccaag ggattgaacc ctgatgacgg cggttggctg cgacggctgc gcggaggtca
     9901 gcaccggcag gagggacgcc gatgacccgc gcgatcgacc gcgagtcccc ggagtcgctg
     9961 ccggagcacg gcaggaagac ctcgaagtgg ccgcgcacga cgcggcgcca ggagcgaatc
    10021 cagcacgtcc tggcacggcg ccagacatcg ctgacggtgg tgcttgaaga cgtgcacgac
    10081 ccgcacaacg cgagtgcggt gctgcgcagc tgtgacggcg tcggtatcct tgatgtgcat
    10141 cttgtctaca tgcagcacga accaccgaag cggtcgttcg cccgaacaac ctcgggctcg
    10201 gcggcaaagt ggattcgcac gcactaccac gactccatcc aggcgtgcta caggctcctg
    10261 cgtgagcagg aaatgtgtat ttgcgcgacg gcgcttggtc gcgagagcga tgatctatac
    10321 gcgcttgatc tcacgcagcc gaccgcgctc gtgttcggta acgagatgcg aggcgtctcc
    10381 gaggaggcga tcgcgggcgc ggacaagatg gtgttcatcc cgatgcaagg catggtggag
    10441 agcctcaata tctcggtcgc atgtgccgtg acgctctacg aggcgatgcg ccagcgaatc
    10501 gcggcgggcg catacgaatc tccggagctc agcgccgagc aactcgccag cctcgaacaa
    10561 gagtggctca ggaagtaaga gaggcgcggc ggaacaggca ttgggctgca agccgagtag
    10621 agccggccgc tggccagcag ggggaatgga tctactggtc cccgtcgaca cgctcgatct
    10681 gcataccggc ggcgaggcgc tcggggatcg catgcctgcc accaccgagc cggacttcca
    10741 catctgaccc agtgtcgccg gcgcggtcga gcacttggaa cgtgacgcct ggtgtaaggc
    10801 cgatcgtatt gaggaacctt agatctttgg catcacgatc gctcacgcgg cgcaggatta
    10861 cccgagatcc tgcggattcc tccgtgaggg cgtgccactc ggcgcttggc agctcgccgt
    10921 gaaggctagg gatcgggtcg ccgtgcggat cgtactccgg gtgcccgagc agctcatcca
    10981 ttcgagactc gaactcgctc gagacatggt gctcgagccg ctcagcctcc tcgtgcacct
    11041 tgtcccagtc gtatcccatt gcctcggcca ggtagagctc caacagccgg tggtgccgaa
    11101 tcacctccag cgccaccttg gcgccggcct tggtcaggcg caccccgtag tagcggctgt
    11161 gttcgagcag ggagacctcg gccaagcgct tcaccatgtt cgtgaccgat gctgcagaga
    11221 ggccgagctc ggtggcaagg gcctgcgtgg tgactcggcc ctcattctgc tgcaggcggt
    11281 gaatcgcctt caggtaatcc tccattgcgg cgctgatcgc cggggtggcg cgaccgagcg
    11341 aggtatgtcg ggcgtggttg tcgggcgaag aaggactgga catgcacgca cctcagtcag
    11401 cccgctgggg agagggcatc cgcctgcggg caactgggct atggaaagtt gaagggcagg
    11461 ttctttggca tcttgccctt cttcgccatc tggccaaact gacccatcat cttctgcatc
    11521 tccttgaact gggagagcat ctggttaacc tcctgccggt cccggccagc accacgcgcg
    11581 atgcggcggc ggcgggaatt gctgatcctc tccggatgac ggcgctccca cggagtcatc
    11641 gactggatga tcgcttcgat cctcttgtag tcatcgtcgg aaacctgggt tcgggcctga
    11701 cgcatctggg caccgagacc cgggatcatg tcgagcagct ggtcgagcgg acccatcgac
    11761 ttgatcttct gcagttggtc gaggaagtcc tcgaggtcga actgtccttt catcatgcgg
    11821 tcttgcaggc gctcggcgtc ctcttcggtc gtctgctcct gtgcgcgctc gatgagggag
    11881 agaacgtcgc ccatgccgag aatgcggctg gcaaggcgat ccgggtagaa gggctcgaga
    11941 gcgtcaagct tctcgccagt gccgatgaac ttgatcggca cgccggtgac cgcccggatc
    12001 gagagcgccg caccaccacg ggcgtcgcca tccatcttgg tcaacacaag gccggtgacg
    12061 tcgagttggt cgtgaaaggc ctcggcgaca ccgacggcct cctgaccggt cattgcgtct
    12121 gcgacaagca ggatgtcgga gggggtaacc cgctccttga tgttgaccag ctcctgcatg
    12181 agacgctcgt cgatctgcag tcgaccggcc gtgtcgatga tgaccggatt gaagcctcgc
    12241 tcctgcgcga agcgcatggc gttccgggcg atgtcaacgg gatcgctctc ggtgccctcg
    12301 tcatagaccg gcatgtcgat ctgcttgcca agcgcctgca gttggttgac cgccgccggc
    12361 cggtagacat ccgcagcgac catcaacggg ttacggccct gcttgcgcat atgcagcgcc
    12421 agcttacccg tgtgggtggt cttgccggat ccctgcaagc cgaccatcat gattgtctgc
    12481 ggcgggcggt ccggcgtgtt gagcggcacc cgctcctcgc cgaggatctt gatcaactcc
    12541 tcgttgacga tcccgatggc cgtctgcgcg ggcgtgagcg actgcatcac gtcctgcccg
    12601 agcgcgcgct ccttgacgct ggcgacgaat tgcttgacga ccttgaagtt gacatccgcc
    12661 tcgagcaggg cccggcggac atcacgcatc gcctcatcga catccttctc ggtgaggcgt
    12721 cccttccggc cgagtttggc gaacgtgtca ttcaggcgat cggatagtgt ctcgaacatc
    12781 ggctagaatc cctccccttc gccaaagaag agcgaatcca cgtacgtttc cggattgaac
    12841 tccgcgaggt ccgttgcgcg ctcgccagtg ccgatgtagg cgatcggcgt gccgatttcc
    12901 ttggcgacgg agaaggcgat gccgcccttg gccgtgccgt ccagcttggt gatcatgagg
    12961 tcggtaattt cgaccgactt ggtgaactcc gccgcctgca ccaggccatt ctgaccggtg
    13021 gtcgcatcga gcaccaggat cacctcctgc ggcgcctccg gaatctgctt ctggatgatc
    13081 ttgcgaagct tttggagctc agccatcaag ttgtgcttgg tctgcaggcg accagcggtg
    13141 tcgatgatca gtacgtcgac gccccgctgc tgtgcggact ggaccgagtc gaaaacgacg
    13201 gccccgggat cggcaccctg ctgatgggcg acgaccggca cgtctacgcg ctcgccccac
    13261 accttgatct ggtcaattgc agcggcgcgg aaggtatcgc ccgcggccag catgacggag
    13321 cggccgaatc gcttgtggta ctgggccagc ttggcgatcg atgtcgtctt gccggtgccg
    13381 ttgacgccag ccacgaggat gacgaacggc acaccgcgct ggagcagctt gatcttgcgg
    13441 ttctgcgtcg caagctccag cagcttgatc atctcgagcc gaaggatctc acgggcatcg
    13501 gccgggttgg tgatgtgatc ctcctggata cggtcctgaa gctcctggac gagctgctgg
    13561 ctgacatccc agcccacatc ggcctgaatc agcagcatct caaggtcttc gaaaagctcg
    13621 tcgtcaagtt cggcccggtc gaagagcttg gtgatctcgc gaaagacccc cttgcgcgtc
    13681 ttctccagac cctgttccat ctggacgtca ggctcctgac gccgcttgaa catgcgcttt
    13741 agaatcattg actgccaccc tgatgtgtcg ttcgggacgt tgtgacgggt tgtgtgggag
    13801 gtcccgaatg tgcgaaaaaa acggcttgtt gcagccacca aaagtataca acggtgtatc
    13861 tgcccggctt cacaaccgcg acagtgaacg agccacctgc taggatgcgc ttctgacggg
    13921 actcggtttg catcgacggg aaggacggga agatggaccg acgggagctg ctactcgcgg
    13981 tatatcttcc caccgtgctc ctcgccttca cccaaggcgt gatcgtcctg tcgctgccgt
    14041 tgctcgccaa cgagacctcc gacgcctaca gctttgcgag catcatcgtc tcggcggcag
    14101 cattcggcac gctgatcgcc gatgtgccca ccggcacggt gatgctgcgg ctgggcctgc
    14161 gtcgcaccat gctgattggc tcactgctcg tcgcactgtc gacatgggcg ctggccatcc
    14221 cgctcgacgc gccggccgtg ctggcgctcc ggttggctgc cggtatcggc actgcgctct
    14281 gggggctttc tcgctacacc tacatcaccc agaagatacc gccggaattg cgtggccgga
    14341 ccattgccgg attcgggggt atcaatcgca ttggagtctt tgccgggccg atgatcgccg
    14401 ggtcgctggt gaccgcatat ggcctctaca gcgcgttcct gctggcaggc acgctcgctg
    14461 cgttcgcttt cgccgccgcc tggcgctgga ttcccggcga tgctgtagcc cccaggccgc
    14521 ctgatgccgc tcaccatgcc ccggacacgg gagagatccg gggcacctgg aacgctatcg
    14581 ctggtaaccg gtccgacgtg ctcttcggcg cgctcgcaca ggtcctcgcg caagtggtgc
    14641 gtcagggccg gcacttcctt atccccctct acggcgtcga ggtcctgtca ctcgaggcgg
    14701 tcgaagtcgg tctcgtgatg acggcgtccg cggtgatcga catggtgatg ttcggccccg
    14761 ccgggttcct gatggaccgc ttcggccgga agttcgcgac gatcccgagt ttctccgtga
    14821 tggctgtcgg cattgccatg atcccgttcg ccggagggtt caccgggttg ctggccgcgg
    14881 gcatcgtgat cgggttcggc aatgggctgg gttcggggac catgatgacc ctcggtgccg
    14941 atttcgcgcc gtccgcgtac accagcacct tcctcagcct ctggcgcttc atcggcgaca
    15001 gtggccagat gatcgggccg attctcgtcg gcgtggttgc ccaactcttc acgctgcggc
    15061 aaagcgcgtg ggtgctctca ggctgctcgg tgacatgcgc attgatgctc ttcttcctcg
    15121 tgcgtgagac gcggcgggaa cacggatcgc ttgaggcgac cgaggagggg acggagccgg
    15181 agccggtgtt gcagaaacag ggaggatagc agatgccact cgtggccgcg gcgatcgcgc
    15241 cgcacggttt cccggtcatt cctgcactcg gagacgatgt cgagggcgaa ggcgaaaccc
    15301 ggccggcgct cgccgcgatg ggggagttct tccgtcagca gggggtcgag gcagttgtga
    15361 tcgccgggcc gcatggcatc cgcgccgacg gcttcttcgc cgtggttgac gccgcacgcg
    15421 ccgccggcac gctgcactgg aaggggagac aggtcgagct gaacgcgcca tgcgaccgcc
    15481 cgctcatcga ggcgatttcg atgcaggcga gcgacgacgg tttgccggtg gcccgtgtga
    15541 gcttcgctgg caaccgtgcc gaccaggcag tcgtgccgct cgactgggga gcgctggtgc
    15601 cgctctggtt cctcggccat gaccagaacg aagcgaacac agggaatgtg ctcgccgatc
    15661 cgccgagcgc tcccggcggt ccgccggcag tgttgatcac accgtcacgc tccctagacc
    15721 gcaaggcaat ggtggcgttc ggccggtcga tcgcgagcgc gatcgagcgc gacgagcgag
    15781 gggtcgggtt catcgcttcg tgcgactggg cacacacgca tcgcgaggat ggtccgtacg
    15841 gcttccacga ggccgccgat cgggtggatg ccatcgtcgt caatgcaatc aggcggaacg
    15901 cgctgatgga gctgatcgag ttgccgccag aggacgtgga gcacgccgcg atcgatgggc
    15961 tttggcagac gctcatgctg gccggcatcc aggaggtcac gccgtttgat ctgcagtttc
    16021 gctcgtatga ggcgccgacg tactacggca tgatcgtcgc aactacggca gtgggccatg
    16081 actgagatag cgcgggaccg ccctccggtc tactcactca tggttcgaga gatgctcccc
    16141 gacgagcgcc cgcgcgagcg cctgcggatg ttcggggaag ggaacctcaa cacctccgaa
    16201 ctgcaggcga tcatcctgaa caccggcatg aaaggcgaga gcgtgatctc tatgaccaca
    16261 cggctcctgc acgaccaagg tggcctgcac ggcctgtacc ggacgagctt cgacgagctg
    16321 gccagcacca gggggatcgg agagtcgaag gcatgcaaga tcaaggcggc gctggagctg
    16381 ggccgccggc tctcggcagc cgcaccggcg gagcgggtcg cgatcgaagg gccggacgac
    16441 gtggtgcgat tggtcggtgt cgagatggcc gctcttgaac aggagcagct gcgcgtcgtg
    16501 ctgcttgaca cgcgacacca cgtgatacgc caccggacga tttacaccgg cagcgtcaac
    16561 agtgcgcagg tacggccagg cgagttgttt cgcgacgccg tccgagccaa tgcgaccgca
    16621 gtgatctttg cgcacaaccg tccatctggt gacccgacgc cctcggtcgc cgatattgaa
    16681 ttgaccgccg acgtggtgaa aagcggacaa ttgctggaca tcgccgtttt ggaccacgtg
    16741 attatcgggc agggccgctg ggtgtcaatg aagcggctcg ggctcgggtt cgccatcgac
    16801 tgagaccagg aggcaaacat catgacactc gggttcggcg tcacgagtac gatcgaccaa
    16861 gagcgcgtgc aacgccttgc gccagctgtc gaggcagccg ggttcacctt cttctggtcg
    16921 aacgacatcc ccggtggctc ctcgttcgat gtgctggctg cggccgccgg tgtgacggag
    16981 accatgagcc tcgccaacgg tgtagtgccg atcgaccggg tgccgcccga ggcgtggcag
    17041 gatcgtatcc gggaggcggg cctggccggc gatcgattct ggccagggct cggcagcggg
    17101 cggatccaca agccgctgga gccgatggga gaggcgatcg agactatcca gcgcaccacc
    17161 ggccggccgg tcgtgatcgg cgcgctgcgc tcccgcatgc gtcggctcgc cgcagcacgg
    17221 gcagacggcg tgcttctcaa ctggctgacg cccgcagcag ccgcggtgcg gcgagaggag
    17281 atgagggata tccgccggga ggcagcggtc acccgtccct tcctggccgc tgcctatatc
    17341 cgggtcgcga tcggcgatgc ggcccttgac cggttgcggc atgaggccga gaagtatgaa
    17401 tccttcccat cctacgcgaa gcatttcgct gagatgggcg ccggcgcctt cgagacggca
    17461 gtggctgcaa catcagtcga agaggcgtcc caggccctcg tgccgttcat cggcgcactg
    17521 gatcacgccg tgctgagatg cgtcgtgggt gaggagactg acgatgccta cctgcagctc
    17581 gtggaagctg gacgcctcgc gatcgagcgt gccacccaag aataggccgc catccctctg
    17641 accgggccgg atgctatcct tgtacagttg gtctggcaaa agcctgccag ctgccacgca
    17701 acgcgcggtc agaacccgca gtatgagcca ccgatcgtcg atcgggaggg ttttttgtgt
    17761 caaaacagat cggcatcgac ctcgggacag cgaatgtcct cgtcta
//
LOCUS       DASYWL010000207         3186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_10146, whole genome shotgun sequence.
ACCESSION   DASYWL010000207 DASYWL010000000
VERSION     DASYWL010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10146"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="VGR22_07290"
     CDS             <1..1009
                     /locus_tag="VGR22_07290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEV2128403.1"
                     /translation="IRGTGDGSGKGKVVLARADMDALPIVEENEVDYRSKNDGVMHAC
                     GHDAHTAMLLGTARTLMDRRDQFAGAVKVLFQPSEEAGEGGAIGMIREGVLEDPHVDA
                     CFGLHVSSRIPTGKISVASGPLNAAADEFKITIQGKGGHGAYPHLCVDPVVIGAEIVV
                     ALQTLVSRDVDPTDKAVVSVCSFKAGEAFNVIPDTAMIGGTVRTFDQETRDLLDTRIH
                     ELATGVAAAMGGSVNIDYRRGYPPVINDEAMAGIVREAAREVVGEENVEMGKPKMGAE
                     DFSYFALERPSCFFNVGTGNAEKETDISHHHPRFNIDEEGMAQGIATMATTLVSFLNR
                     E"
     gene            1257..2462
                     /locus_tag="VGR22_07295"
     CDS             1257..2462
                     /locus_tag="VGR22_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEV2128404.1"
                     /translation="MVVATDFRTDVDEILPGVVADRRHLHENPELGFQEHETAKFVTE
                     RLQSLGVEDIRTGINFTGVTGLVRGTGDRPGKDTVVLVRADMDALPILEENRVDYKST
                     NDGVMHACGHDAHTAMLLGLARILTDRKDSFAGTIKLLFQPAEELPPGGAKGMIEQGV
                     LEDPHVDVVLGLHVSSDTKTGTIKIGDGPIMAAADGYRITIQGKGGHGASPHLCVDPV
                     MVGSQIVVALQTLVSREVDPTDKTVVSNCVFKAGEAFNVIPDTAQLAGTVRTFLPETR
                     DLMEKRITELAQGIAASMGAEAKVDYTRGYPATVNDPGIADMVREAARQVVGEENLEE
                     SAPKMGAEDFSYFLEARPGCFFFVGTGNEEKETNISHHHPRFDVDEDGMAQGLATMAT
                     SVVSVLNQG"
     gene            2574..>3186
                     /locus_tag="VGR22_07300"
     CDS             2574..>3186
                     /locus_tag="VGR22_07300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEV2128405.1"
                     /translation="MAVAADLKRDVDEILPGLVADRRHLHEHPELGLQEHETAKFVTE
                     RLQALGVEDIQTGISETGVTGLIRGTGDGSGKDKVVLARADMDALPIVEENEVDYRSK
                     NDGVMHACGHDAHTAILLAVARVLMDRRDQFAGTLKLCFQPSEETGPGGAKWMIEQGV
                     MQDPPIDACFGLHVWQNEPVGTVLVSDGPVMANSDRFEIRIQGK"
BASE COUNT          596 a          995 c         1083 g          512 t
ORIGIN      
        1 gattcgcggc accggcgatg gttccggcaa gggcaaggtc gtgctggcgc gggccgacat
       61 ggacgcgctg ccgatcgtcg aggagaacga ggtcgactac cgctcgaaga acgatggtgt
      121 gatgcatgct tgcgggcacg acgcgcatac ggcgatgctg ctcggcaccg cccgtacgct
      181 gatggaccgc cgcgatcagt tcgccggcgc ggtcaaggtg ctcttccagc cgagtgagga
      241 ggccggcgag ggcggcgcga tcgggatgat tcgcgagggg gtgctggagg atccgcatgt
      301 cgacgcctgc ttcgggctgc atgtctcctc gcgcattccg actggcaaga tcagcgtcgc
      361 cagcggcccg ctgaacgcgg ctgccgacga gttcaagatc accatccagg gcaagggtgg
      421 gcacggtgcg tacccgcacc tctgcgtcga tccggtcgtg atcggcgcgg aaatcgttgt
      481 tgcgctccag acgctggtct cccgcgatgt cgaccccacc gacaaggccg tcgtcagcgt
      541 gtgctcgttc aaggcgggcg aggcgttcaa cgtcattccc gacaccgcga tgatcggggg
      601 cacggtgcgc accttcgatc aggaaacgcg cgacctgctc gatacgcgta tccacgagct
      661 ggcgaccggc gtcgcggccg cgatgggcgg ctcggtgaac atcgactacc gtcgcggcta
      721 cccgccggtc atcaacgacg aggcgatggc tgggatcgtt cgtgaggcgg cacgcgaggt
      781 ggtcggcgag gagaacgtcg agatgggcaa gccgaagatg ggcgccgagg acttctccta
      841 cttcgcgctg gagcgtccga gctgcttctt caacgtcggc acgggcaatg cggagaagga
      901 gacggacatc tcgcaccatc acccacgctt caacatcgac gaggagggca tggcgcaggg
      961 gatcgccacc atggcgacca ccctcgtgag cttcctcaac cgcgagtagg cggagacgcg
     1021 gtcacccaac cccgtcattt cgagcgtaac ccgagcggag cgagagtgca gtcgagaaat
     1081 ctctcccgtg ttcgtcgcct gattgccggc tggcaagaag cttttcgact ccacgacttc
     1141 cttccaaatg acagggtttg gaatcgactg cggagcttgc ccgaggcatg gtcgcgagca
     1201 gcggatagaa ttgacgagtt acacgcgcgt cacgcttatc gaaggaaacc caaacgatgg
     1261 ttgtcgcaac agattttcga accgacgtcg acgagattct gcccggggta gtcgctgatc
     1321 gccgccacct ccacgagaac ccggagctcg gcttccagga gcacgagacg gcgaagttcg
     1381 tcaccgagcg attgcaatcc ctcggcgtcg aggacatccg caccggcatc aatttcactg
     1441 gtgtgaccgg tctggtccgt ggcaccggcg acaggcccgg caaggacacg gtcgtgctcg
     1501 tccgcgcgga catggatgcc ctcccgatcc tcgaggagaa cagggtcgac tacaagtcga
     1561 cgaatgacgg ggtgatgcac gcttgtggcc atgatgcgca taccgccatg ctcctcgggc
     1621 tggcgcgcat cctcaccgac cgcaaggaca gcttcgccgg gacgatcaag ttgctcttcc
     1681 agccggccga ggagctcccg ccaggtggtg ccaagggcat gatcgagcag ggcgtgctgg
     1741 aggacccgca cgtggacgtc gtcctcgggc tgcacgtctc ctcagacacg aagaccggca
     1801 cgatcaagat cggtgacggg ccgatcatgg ccgccgctga cggctacagg atcacgatcc
     1861 agggcaaggg cggccacggc gccagcccgc atctctgcgt cgacccagtc atggtcgggt
     1921 cgcagatcgt ggtcgcgttg cagaccctcg tctcccgcga ggtcgacccg accgacaaga
     1981 ccgtggtcag caactgtgtc ttcaaggcgg gcgaggcgtt caacgtcatc ccggatacgg
     2041 cgcagctcgc cggcacggtc cgcaccttcc tgccggagac gcgcgacctg atggagaagc
     2101 gaatcaccga gttggcgcag ggcatcgccg cgtcgatggg cgccgaggcg aaggtggact
     2161 acacccgcgg ctacccggcg acggtcaacg accccggcat cgctgacatg gtgcgcgagg
     2221 ccgcccggca ggttgtcggc gaggagaatc tcgaagagtc cgcgccgaag atgggggccg
     2281 aggacttcag ctacttcctc gaggcgcggc cgggctgctt cttcttcgtc ggcacgggca
     2341 atgaggagaa ggagacgaac atctcccacc accacccgcg gttcgatgtc gatgaggacg
     2401 gcatggcaca gggcttggcg acgatggcca ccagcgtcgt gtccgtgctg aaccagggct
     2461 agcctgggcc acgccgtacg ggtggactcc atgtctgcct catccacagc gccggcacgt
     2521 tcgctgcgct caatgcaggc tcttgtgccg gcctatgcaa ggagacccga gccatggccg
     2581 tagccgccga tctgaaacgt gacgttgacg aaatcctgcc gggactggtc gccgaccggc
     2641 gccacctcca tgagcatccc gagctgggct tgcaggagca cgagacggcg aagttcgtga
     2701 cggaacggct ccaggcgctc ggtgttgagg acatccagac gggcatcagc gagactggtg
     2761 tcactggcct cattcgcggc accggcgatg gttccggcaa ggacaaggtc gtgctggcgc
     2821 gggccgacat ggacgcgctg ccgatcgtcg aggagaacga ggtcgactac cgctcgaaga
     2881 acgatggcgt gatgcatgct tgcgggcacg atgcgcatac cgccatcctc ctcgcggttg
     2941 cccgggtgct gatggaccgg cgggaccagt ttgccggcac gctcaagctc tgcttccagc
     3001 cgagcgagga gaccggcccg ggcggcgcga agtggatgat cgagcagggg gtcatgcagg
     3061 atccaccgat cgacgcctgc ttcggactgc acgtatggca gaacgagccg gtcggaacgg
     3121 tgctggtcag cgacggaccg gtgatggcga actcggaccg cttcgagatc aggatccagg
     3181 gcaagg
//
LOCUS       DASYWL010000208         7916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_197568, whole genome shotgun sequence.
ACCESSION   DASYWL010000208 DASYWL010000000
VERSION     DASYWL010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7916
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197568"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /gene="moeB"
                     /locus_tag="VGR22_07305"
     CDS             complement(<1..1151)
                     /gene="moeB"
                     /locus_tag="VGR22_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEV2128406.1"
                     /translation="MPTLQDLLNDARKTVPEPGIDDVRADRPTLIDIREQDEVDQGLA
                     EGAIHIPRGYLELRIEQAVPDRDTPMVLYCAGGTRSLLAAKALQDLGYTHVASLEGGF
                     DAWKKADAPWTTPRTLTAEQRRRYSRHLLLPDVGEAGQAKLLDAKVLLIGAGGLGSPA
                     GLYLAAAGIGTLGIIDDDVVDTSNLQRQVLHTTDRIGEPKAQSARQAIEAINPDVTVI
                     AIEDRLTRENVFDMIEPYDVIVDGSDNFATRYLLNDAAVLRKKPVVHGSIFRFDGQAT
                     VLDPRDETSPCYRCLFQSPPPPELAPNCAEAGVLGVLPGMIGMIQATEAIKLVLGIGE
                     TLVGRLLMYDARSMEFRTLRVKRSPTCPMCGEHGPDSLDGITYDDVSCA"
     gene            1420..2523
                     /gene="msrA"
                     /locus_tag="VGR22_07310"
     CDS             1420..2523
                     /gene="msrA"
                     /locus_tag="VGR22_07310"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEV2128407.1"
                     /translation="MTIKDTWDAIFGHKTKMIDPADALPGREQPNFEISGRHAVLGTD
                     MTPPFPENLEVASFGLGCFWGAERLFWELDGVWTTAVGYAGGYTPNPTYEEACTGKTG
                     HAEAVLVAYDPAEISYEELLKIFFEAHDPTQGMRQQGDIGTQYRSVIFVHNEQQREVA
                     ERVRASYDRALREAGEDPITTEIVEAGPFYYAEEYHQQYLHKVPHGYCGLKGTGVACA
                     IAPAPVSGGDHDTATEDSGLKPLPESEEEWRERLGPEQYKVLREAGTERPFTGHYNAV
                     WEEGTYRCTACGNPLFDSSTKYDHGCGWPSFGEALPGAVEYHEDRSFGMRRTEVRCAR
                     CHSHLGHVFNDGPREYKGARFCMNSVAMDLDRK"
     gene            complement(2876..3184)
                     /locus_tag="VGR22_07315"
     CDS             complement(2876..3184)
                     /locus_tag="VGR22_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128408.1"
                     /translation="MGRPNITEDQWEMAVTMLANAIRKDAHLRGRARRAKERGNVLAS
                     STVGRRTESSQRYVEGMRDILGVLFAEGPELADQCMEEAYAMAMGTPTHFADNGQNYH
                     "
     gene            3547..4524
                     /locus_tag="VGR22_07320"
     CDS             3547..4524
                     /locus_tag="VGR22_07320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEV2128409.1"
                     /translation="MAVKFGVFVPQGWRMDLTGIDNPVEQFEAMTSVAKEADGRTDFD
                     SIWVYDHVHTVPEPTMNTTFECWTITATLARDTKRVNVGQMVTCNGYRNPALFAKIAS
                     TVDVASHGRLYAGFGAGWYEHEWRAYGYGFPETKERMGMFREAVQIIHKMWTEDKPSF
                     SGKRYTIDEPINEPKNAGKKIPLWIGGGGEKVTLKLVAQYGDACNVGGGDAETIQHKL
                     DVLKGHCETEGRNHNEITKSTSYNVLPIGAGVDGEKATEKLRATLGDIPWADFAESTV
                     VGPPEQIAEKIQSGVDAGINYAINYIPGLAYDTEPMHRYNEEVIPLIKG"
     gene            4553..4906
                     /locus_tag="VGR22_07325"
     CDS             4553..4906
                     /locus_tag="VGR22_07325"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV2128410.1"
                     /translation="MAHDRLDILTLGGTPSSSDAPHLLGSLASDDLNVNLMRFAGENG
                     IDEHVNREVDVLIVVLDGEGVVTVGGADTVLGAGRATIIPKGTARAIRSNGKRFAYLT
                     CHGQRRGMMPTVDGG"
     gene            complement(5081..6052)
                     /locus_tag="VGR22_07330"
     CDS             complement(5081..6052)
                     /locus_tag="VGR22_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128411.1"
                     /translation="MTKPRRSAKPALSTSTSIVLTRSAEEEAWHDMQATIRKRRLRIR
                     KLQAEIRPLEEALARFERNYRVQLGGLQDELRLIRSEIHTLQDRTARIHARMVSDPDD
                     ELGDLFSPEELEEIGRFFNFEIPDSWFRGEDAEEPSGDGAFEDEHFDASGEGWHRIEE
                     PEERQASHARLEELKALYRSLVRRFHPDLCREKADEAYRHECMLRANHAWKAGDLESL
                     QELADETERLALDWGSWTWLRRLQSARRENDRITRQISKLRRRLKELRTSETFPLWSS
                     GGLGESLISARRKSLERDIARAKDELEEARSAFRTAMEMWADQKATA"
     gene            6191..6379
                     /locus_tag="VGR22_07335"
     CDS             6191..6379
                     /locus_tag="VGR22_07335"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEV2128412.1"
                     /translation="MSHGNDIGTRKMFGEYGVDCGEVFVGVICDAKFYLKITDAGREM
                     LSNAGRRDRPTEVRNRIW"
     gene            complement(6688..7701)
                     /locus_tag="VGR22_07340"
     CDS             complement(6688..7701)
                     /locus_tag="VGR22_07340"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2128413.1"
                     /translation="MVLVFLVNGTVLASWVSRIPAITEKHGLGKGEVGTLLMTIAVDA
                     IISFAIAGMGINRNGSAKATTIFGSLFALMLPLIGLVPNVWMLVPVFVLYGAANGGMD
                     VAMNAQGVEVEQALRRSTINSLHGFFSLGGVIGAAIGALAAWLDIAPSIHLPAVGLLG
                     VAGLWLLRSHLIPDEHLSHTSTDEPVFALPPKAMWPLGVIAFCSAIAEGAIADWSALY
                     LDDHLDIGGGVAALGFAAFSLSMLIGRFSGDALVDRFGSVAMVRVGATISSVGLAVSL
                     LINVPAAMIVAFGLVGLGVAVLFPLVFKSAARFPGVSRGRAVAGVATIGYTGFLIGPP
                     VLG"
BASE COUNT         1398 a         2523 c         2644 g         1351 t
ORIGIN      
        1 atcgcacagg agacgtcgtc gtaggtaatc ccgtccagcg aatcggggcc gtgctcgccg
       61 cacatcgggc aggtcggcga tcgcttcacc cgcaacgtgc ggaactccat cgagcgcgca
      121 tcgtacatca gcagccgccc gaccagcgtc tccccgatgc cgagcaccag cttgatcgcc
      181 tcggtcgcct ggatcatccc gatcatcccg ggcagcacgc ccagcacgcc cgcctcggcg
      241 cagttcggcg ccagctccgg gggaggcggg gattggaaga ggcagcggta gcagggactc
      301 gtctcgtcgc gcggatcgag caccgtcgcc tggccgtcga accggaagat gctgccgtgc
      361 accaccggct tcttgcgcag caccgccgcg tcattgagca ggtagcgcgt ggcgaagtta
      421 tcagacccgt cgacgatcac gtcgtacggc tcgatcatgt cgaagacgtt ctctcgagtc
      481 agtcgatcct cgatcgcgat cacggtgacg tccgggttga tcgcctcgat cgcctgtcgc
      541 gccgactgcg ccttcggctc gccgatgcgg tcggtcgtgt gcagcacctg gcgctgcagg
      601 ttgctcgtgt cgaccacgtc gtcgtcgatg atgccgaggg tcccgatgcc cgccgctgcg
      661 aggtagagtc cggccgggga gccgagcccg cctgcgccga tgagcagcac cttcgcgtcg
      721 agcagctttg cctgccccgc ctcgccgacg tccggcagca gcaggtgtcg gctgtagcgt
      781 cgtcgctgct cggcggtcag cgtgcgaggc gtcgtccatg gtgcgtcggc cttcttccat
      841 gcgtcgaagc ccccctccag agaggcgacg tgggtgtagc cgaggtcctg gagcgccttc
      901 gccgccagca gcgagcgtgt gccaccggcg cagtagagca ccatcggtgt gtcccggtcc
      961 ggcaccgcct gttcgatccg cagctcgagg tacccgcgcg ggatgtggat cgcgccctcg
     1021 gcgagcccct ggtcgacctc gtcctgctcg cggatgtcga tgagcgtggg gcggtcggca
     1081 cgaacgtcgt cgatgccggg ctccggcacg gtctttcggg cgtcgtttag gagatcctgc
     1141 agggttggca tggcgatgga gcctcccgtc gtgacaacgg gcatcacggc ccgtctcggc
     1201 tggcctgctt acccgattgt gccacgatcc cggacaatcg atgtcccgct cagggcgtca
     1261 gcgggctgga gcgtgtgccg cgttacacac cagagtgccg ccgtggtgtg tcggttgcga
     1321 caaccgcgta tagtgcaggg agatgtgcat caggaggcct ccctgatttc agcgagtgcc
     1381 ccgcaacaaa cgctccacac gtgagcgaga ggagcgaaca tgacgatcaa ggatacatgg
     1441 gatgccatct tcggccacaa gacgaagatg atcgacccag ccgacgcgct gcccggtcgc
     1501 gagcagccga acttcgagat ttccggccgg catgcggtgc tcggcaccga catgaccccg
     1561 cctttccctg agaacctcga ggtcgcatcg tttggcctcg ggtgcttctg gggcgcggag
     1621 cggctcttct gggagctcga cggcgtctgg accaccgcgg tgggctacgc gggcggctac
     1681 acgccgaacc cgacctacga ggaggcctgc accggcaaga ccggtcacgc cgaggcggtg
     1741 ctggtggcct acgacccggc ggagatcagt tacgaggagc tgctgaagat cttcttcgag
     1801 gcgcacgacc cgacgcaggg aatgcgccag cagggggaca ttggcacgca gtaccgctcg
     1861 gtgatcttcg tccacaacga gcagcagcgg gaggtcgccg agcgggtgcg agcaagctat
     1921 gaccgtgccc tgcgggaggc cggcgaggat ccgatcacga cggagatcgt cgaggcagga
     1981 ccgttctact acgccgagga gtaccaccag cagtacctgc acaaggtgcc gcacggttac
     2041 tgcggcctga agggcaccgg agtcgcctgc gcgatcgccc cagcaccggt gagcggtggc
     2101 gatcacgaca ccgcgaccga ggattcgggc ctgaagccgc tccccgagtc ggaggaggag
     2161 tggcgcgagc ggctcggtcc ggagcagtac aaggtgctgc gcgaggctgg gacggagcgt
     2221 cccttcacgg ggcactacaa cgctgtctgg gaggagggga cataccggtg caccgcctgc
     2281 ggcaacccgc tcttcgacag cagcaccaag tacgaccacg gctgcggctg gccgagcttc
     2341 ggcgaggcgc tgcccggcgc agtggagtac cacgaggacc gctcgttcgg catgcgccgc
     2401 acggaggtcc gctgcgctcg ctgccacagc catctggggc acgtcttcaa cgacggcccg
     2461 cgcgagtaca agggcgcccg gttctgcatg aacagcgtcg ccatggacct cgaccggaag
     2521 tagggacggg tcagaatcag atcgggttat caaaggcaaa acgggcggac ttggggtccg
     2581 ctcgttttgc ttgctatgtg atcggctggg aggatcgtgt cactcagcgc tgtcctttcg
     2641 agtccattcg actccggcct gcggccgtcg ctcaggacag gtttcgcggc gcggccgtgc
     2701 agccgagaaa tctctttggg catcgggatt aggtcccact tcgcaagaga tttctccact
     2761 tcgctcccta acgggtgctc cggtcaaaat gacaggacgg ctgtttctac agggcaaacc
     2821 ggggggagcc ggcttggccc catcgagaag gtttgggtca ggcaaccgat cttgatcagt
     2881 gatagttctg gccgttgtca gcaaagtggg ttggggtacc catcgccatg gcgtaggcct
     2941 cctccatgca ctggtcggca agctctggcc cctcggcgaa cagtaccccc aggatgtcgc
     3001 gcatgccctc cacataccgc tgcgatgact cggtccgccg gcccaccgtc gacgacgcca
     3061 gtacattccc gcgctccttg gcgcgcctcg cgcggccccg caggtgtgcg tccttgcgga
     3121 tcgcgttggc gagcatcgtc accgccattt cccactggtc ttcggtgatg ttcggtcgtc
     3181 ccatgcagca gctctcccag cgttccgtgc gcgatctcaa ctgcgatccg tggcgcagat
     3241 gatgcgccac tgacgcgcgg ttctgcacca tgctcggatc gggtacgccc atggtaccgc
     3301 gtggtcctgt gggcgttagt ggcgtgtgtc tgtgcctcgc cgcatgcacg gttgtaggtc
     3361 gtggcacagg tgctacgcca tcccggaatg ccggcttgtg gctggtcagc agtttgcgat
     3421 gatgcccagc ggggttgctg cggcaggccg acctgacgat caggctggtg gcgagagaac
     3481 tgccctccat cgtgcgtgcc gggcaggccg attcggctac cggctcgaat tgacaggagc
     3541 acatgcatgg ctgtcaagtt tggtgttttc gtcccgcagg gatggcgcat ggatctcacc
     3601 gggattgaca atccagtcga acagttcgag gcgatgacca gcgttgcgaa agaggccgac
     3661 gggcggaccg atttcgactc gatctgggtc tatgaccacg tccacacggt gccggaaccg
     3721 acgatgaaca cgacgttcga gtgctggacg atcaccgcca cgctcgctcg cgacacgaag
     3781 cgggtcaacg tcggtcagat ggtgacctgc aacggctacc gcaacccggc gctcttcgcc
     3841 aagatcgcca gcacggtcga tgtcgccagc cacggtcgtc tctatgccgg tttcggtgcc
     3901 ggttggtacg agcatgagtg gcgtgcctac ggctacggtt tcccggagac gaaggagcgc
     3961 atgggcatgt tccgcgaggc ggtgcagatc atccacaaga tgtggaccga ggacaagccg
     4021 agcttctccg gcaagcgcta cacgatcgac gagccgatca acgagccgaa gaacgcgggc
     4081 aagaagattc cgctgtggat cggcggtggt ggcgagaagg tgacgctcaa gctggtcgcc
     4141 cagtacggcg atgcctgcaa cgtcggtggc ggcgacgcgg aaacgatcca gcacaagctg
     4201 gacgtgctga agggccactg cgagaccgag ggtcgcaatc acaacgagat tacgaagtcg
     4261 accagctaca acgtgctgcc aatcggtgct ggggtcgacg gcgagaaggc gacggagaag
     4321 ctgcgcgcga ccctcggcga tattccgtgg gccgattttg ccgagagcac ggtcgtcggc
     4381 ccgcctgaac agattgccga gaagatccag tcgggcgtcg atgccggcat caactacgcg
     4441 atcaactaca ttcccggcct cgcctacgac accgagccga tgcaccggta caacgaggaa
     4501 gtgatccccc tcatcaaggg gtagggcggc gagcgcattg agaggtgacg gtatggcgca
     4561 cgaccgactg gatattctga ccctcggggg tactccgtcg tccagcgatg ccccgcatct
     4621 gttgggatcg ctggcaagcg atgacctgaa cgtcaacctg atgcgcttcg cgggcgagaa
     4681 cggtattgat gagcatgtca accgcgaggt cgacgtgctc atcgtcgttc tggacggtga
     4741 gggggtcgtg acggtcggtg gcgctgatac agtgcttggg gcaggacggg cgacgatcat
     4801 cccgaagggg acggcgcggg cgatccgctc gaacggcaag cggttcgcct acctgacctg
     4861 ccacggccag cgccgcggca tgatgccgac cgttgatggt ggatgacgtt tgctgatccg
     4921 agacaccctg gtcgtcccgc cccgcgcgtg ggtgccgcat ccaccctgcc ggttggttct
     4981 ggcatccgtt cgcggacaca cgagcatgac cccgggcact tgcggaccag ggctactacc
     5041 tggtggtgcg ctgtaatcag ccatcgatgc tttgttcctg tcaggcggtg gccttctggt
     5101 cggcccacat ctccatcgcc gtccggaagg cggaccgggc ctcttccagc tcgtccttcg
     5161 cccgggcgat gtcgcgctcg agcgacttcc gacgggccga gatcagcgat tcgcccaggc
     5221 cgccgcttga ccacagcggg aaggtctcgc tcgtccgcag ctccttgagg cgccgccgca
     5281 gcttgctgat ctgccgagtg atgcggtcgt tctcgcggcg ggccgactgc agccttcgca
     5341 gccaggtcca gcttccccag tccagcgcga gacgctcggt ctcgtccgcg agctcctgca
     5401 gcgactccag gtcacctgcc ttccaggcat ggtttgcgcg aagcatgcac tcgtggcggt
     5461 aggcttcgtc cgccttctcc cgacagagat ccgggtggaa ccgtcgcacg agcgaccggt
     5521 agagagcctt cagctcctcc aggcgcgcgt gactggcctg gcgctcttcc ggctcctcga
     5581 tacgatgcca tccctcgccg gaggcgtcga agtgctcatc ctcgaacgcg ccatctccgc
     5641 tcggctcctc ggcgtcctcg ccccgaaacc acgaatcggg gatctcgaag ttgaagaacc
     5701 tgccaatctc ctccagctcc tcaggcgaga agagatcgcc cagctcgtcg tcggggtcgg
     5761 aaaccatgcg cgcgtgaatg cgcgcggtgc gatcctgcag ggtgtggatc tcggaccgga
     5821 tcagccgaag ctcgtcctgc aggccgccga gctgcacccg gtagttgcgc tcgaagcgcg
     5881 cgagcgcctc ctccagcggg cggatctccg cctgcagctt gcgaatgcgc agccgccgct
     5941 tccggatcgt cgcctgcatg tcatgccagg cttcttcctc ggcggatcga gtcaggacga
     6001 tcgaggtgga cgttgagagg gccggtttgg cggagcgtcg tggtttcgtc atgaggacaa
     6061 taatacctaa cctgtcgggt ccctgactgt tccgttaggt gcgccgcttc agcagtttga
     6121 aacggcgtcg aaggagaact atacgggtca tgccatctga tcagccaacg tcgatgagct
     6181 gcaggagcag atgtctcacg gcaatgatat cggtaccaga aagatgttcg gtgagtatgg
     6241 cgttgactgt ggcgaggtgt tcgtcggagt gatctgcgac gccaaattct acctgaagat
     6301 caccgacgcc gggcgggaga tgttgtcgaa tgcggggagg agggatcgcc ctaccgaggt
     6361 gcgaaaccgc atctggtgat tccggaggac gttgccgagg acggtgaccg cctgagcgag
     6421 atcgttcgcg tcaccgcccc aggccctccc gccgccgaag ccgcggagga agcgcacgtc
     6481 tggcgaattg taggggtaga tccccttggc agcagaggcc gaaccttccc caccaccggg
     6541 ccgcgctaca tatcaccctg ccgtcgcggc cgcttcgccg gacggcgcgg tagcctcgtg
     6601 cccggttgcc gaagcgagca gccccaccgc cgcacagagc actaccacga tagccagcga
     6661 gacgcgcagc gaggtgggct cagcgatcta gcccagtacc ggtggtccga tcaggaagcc
     6721 ggtgtagcca atcgtcgcca cgccagccac cgcccgcccg cgagagacac cgggaaacct
     6781 cgccgctgac ttgaagacga gtgggaagag caccgccacg cccagcccga ccagcccgaa
     6841 tgcgacgatc attgccgccg ggacgttgat cagcaggctg acggccagtc ccacagagct
     6901 gatcgtcgcg ccgacgcgca ccattgccac cgagccgaag cggtcaacaa gcgcgtcgcc
     6961 ggagaaccgc ccaatcagca tggagagcga gaaggccgcg aatccgagcg ccgcgactcc
     7021 gccaccgatg tcgaggtggt cgtcgagata gagcgccgac cagtccgcta tggcgccctc
     7081 ggcgatcgcc gagcagaagg cgatcacacc caggggccac atcgctttcg gcggcaacgc
     7141 aaagaccggc tcgtcggtgc tggtgtgact gagatgctcg tcggggatca ggtgcgatcg
     7201 cagcagccag agacccgcaa caccgagcag gcccactgcc ggcaggtgga tcgacggcgc
     7261 aatatcgagc catgccgcaa gcgcgccgat cgctgccccg atcactccgc cgaggctgaa
     7321 gaagccatgg agcgagttga tggtgctgcg ccggagggcc tgctccacct cgaccccctg
     7381 cgcgttcatg gcgacgtcca tgccgccgtt ggcggcgccg tagagcacga agacggggac
     7441 aagcatccag acgtttggca ccaggccgat cagcggcagc atcagcgcga agagcgagcc
     7501 gaagatggtg gtggccttcg ccgacccgtt gcggttgatg cccatcccgg cgatcgcgaa
     7561 ggagatgatc gcgtcgacgg cgatggtcat cagcagcgtg ccgacctcgc ctttgcccag
     7621 cccgtgcttc tcagtgatgg ccgggatgcg ggagacccag gaggcgagca cggtcccgtt
     7681 gacgaggaag acgagcacca cggcgctgag tgcgcgctcg gcgggtgggg attcggccgg
     7741 agccggagca ttcgatagat cgagcgaaga gtggtcacga gggcatgcac ctggggccgg
     7801 acgggaacgg tccggcaggt ttgtgggacc atcggcagga tacgcgacaa ccgggtgtgg
     7861 aagtagacag tcagtcctgt tcggatgtca ccgattgtgt catttcgacc gaagtc
//
LOCUS       DASYWL010000209        10062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_572747, whole genome shotgun sequence.
ACCESSION   DASYWL010000209 DASYWL010000000
VERSION     DASYWL010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10062
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572747"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="VGR22_07345"
     CDS             <1..766
                     /locus_tag="VGR22_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2128414.1"
                     /translation="VNAPFAYPTSQGPSADHVMGTDSLGQDLLARIFYGGRISLSVGI
                     VAALVAVTVGTAIGAAAGFFGGVTDGLLMRFTDMFISLPQLPLLLLVTFLFRDSMVSF
                     FDDQFGTGNFGVFFLIVLVIGLLAWMPTARLVRASFLSVKEKEFVEAATSIGATQRSL
                     IARHILPNVLSPIIVAATIQVGAAIITESALSFLGLGFPSDVPTWGQMLFQAKDYLGI
                     NMWEAIFPGTMIFLAVLAINYMGDGLRDALDPRKTS"
     gene            859..1161
                     /locus_tag="VGR22_07350"
     CDS             859..1161
                     /locus_tag="VGR22_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128415.1"
                     /translation="MSAATHAQREGSFVWNDHGRFVEVHPVDTGWLVVWGHYEDMGAV
                     CKNEGSVTYATLAGARRRLADAALELTRSPQAAASAVQLFGFHTFPEHTGTLPEPL"
     gene            complement(1339..2097)
                     /locus_tag="VGR22_07355"
     CDS             complement(1339..2097)
                     /locus_tag="VGR22_07355"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2128416.1"
                     /translation="MTSRQSPSSALAFTRAISSSGEPIPQVRQQGKVPVIGLVSAYRY
                     QGLRLSTRLEPIPGYSTVAMSARDVIMPRPAIAGEMVPPCNLLIVEPGDLPRATLGEL
                     VAVAQQHDLPLIWLGEPPIAQRLATATLPGGMLPPQSTLEQIERTLRAAETGLNVIHP
                     DFAHVVDQTEHDVEDDAPLLDDLPVLSPREQEVLTQVANGLPNKAIARVLGITDHTVK
                     FHISSVLSKLDASSRSEAVAIAARRGLLADSGEP"
     gene            2417..3406
                     /locus_tag="VGR22_07360"
     CDS             2417..3406
                     /locus_tag="VGR22_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2128417.1"
                     /translation="MPLPTTLDRSEPDTTTPVIEVRNLRRSFGSFLALDDITMTVPRA
                     SVGLLGANGAGKTTLIRTLLGLLAPDRGEATVVGYDIRSEGTSLRAYVGYMPEAEGLP
                     SNTTAADFVGHMAEMSGIPPRAARQRAADVLYQVGLAEERYRLIKGFSTGMRQRVKLA
                     QAIVHDPQLVFLDEPTAGLDPRGREEMLELITRIYRQLGIAVVVSTHILEDVEKVCDY
                     VLILDAGRLVAAQPLGAMEVIGGEVTVRIDGDQAAFRSGLEAQGVSVHDGGPETPDEL
                     SVDVHDEAVYDVIRDLAVQQGAAIRSMRSMSRSLEDIYLGNVGASREEVGAGV"
     gene            3399..4298
                     /locus_tag="VGR22_07365"
     CDS             3399..4298
                     /locus_tag="VGR22_07365"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV2128418.1"
                     /translation="MSEHANGVAPGRYGEVFDRGYAHYDGPREGRRRAVKSLFVYSIK
                     RAMGIRKSWTAKVLPVLLYVSSFVPLIVMIGIAALLPDANVASYPDYFDGIFFLVGIF
                     AGTVIPEILIPDRQEKTLPLYFARAITRFDYVIAKLAAAAVLTMTISVLPAIILWLGR
                     QLTAESPWSAMRDNIGDLGQVIVLGTLIALTLGTIALVISAMTDRKGVAIAVIVIAFL
                     IVSGLVELALTELDQDWRRYLAFGHMQLVFSAISASLFNAEPNMDVQEADLPTWMYYA
                     WICFIIIAGSLFVRWRYSPGNTA"
     gene            4295..5296
                     /locus_tag="VGR22_07370"
     CDS             4295..5296
                     /locus_tag="VGR22_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2128419.1"
                     /translation="MIGRRRVTTPEPDGQADEPHVIPGLQPGQEPTVLVSEVSKWYGD
                     IVAVSNVTFGVAPGVTGLLGPNGAGKSTLLKMICGLQSTSAGEIRIAGQPVRGRPVVY
                     RKLGLVSEQETIYPFLSGREFVTLNARLQKVPNIGDAVERAIDLVEMGPAAERKVGGY
                     SKGMRQRIKVAGALVHDPDIILMDEPLNGTDPVQRVHLIRLIRDLGRSGKTVIVSSHV
                     LEEVERFAENIIVIVNGRLAAVGDFRAIRERIDSHDREIRLRADRPRELASALIGSEA
                     VRAVRIDEQGRILADTRDVRAFYKVVPALAQRSGVRLYEIQALDESLASVFADLVEG"
     gene            5569..6318
                     /locus_tag="VGR22_07375"
     CDS             5569..6318
                     /locus_tag="VGR22_07375"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV2128420.1"
                     /translation="MTGTLTIMQLTIHQFLRAKSVLVVAAICAIPIFLALILRMANPN
                     LSVVDMRETIGEGIFLGLFSSTLLPLAALVLSTAALGDELDDKTLHYLALKPVSRLRI
                     VIEKYIGTLLITVPIAWGALLAVWLVVSWGEIDATRDLPWPMLVASIGGVAGFAALFQ
                     LVSLYVPRALLVGIFYVFVWEATVSQFLPGIRTISIRHYMQSIFVRLAGDPEITLDGV
                     SRLSTASITIVVIIVLSLLLSARRLRRMNLD"
     gene            6391..7332
                     /locus_tag="VGR22_07380"
     CDS             6391..7332
                     /locus_tag="VGR22_07380"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV2128421.1"
                     /translation="MISHDLMAVNHWVADSRPMGIGLMMPISEGAAFGDTPHFSDIIE
                     MAEVARDVGVDTLWFADHFTFGTPEEGQRGVWEAFTLMAGVAARVPDLNIGSLVSCTG
                     FRNPGVIAKMTETIDDISNGRFILGLGAGWHKPEYDQFGMPFDHRVTRFEEAIEIIHP
                     LLRRGKASSEGRFYSAQGAVNQPRGPRPQGAPILVGSTGDRMLGITVKYADAWNTVWH
                     KEAEEVRPLMDKVDQACRHAGRDPQTLVRAVGGNVAMEGYLGRRPNPIEGDDEAKAEA
                     IAGFREVGMRHFVAGLDPSTPQSIEQFGRVIEILDRS"
     gene            7424..8416
                     /locus_tag="VGR22_07385"
     CDS             7424..8416
                     /locus_tag="VGR22_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV2128422.1"
                     /translation="MGRLAIGVDFGGTKVLASVVDVDTGKIKGTGKKKTSATDGPDEL
                     MDRIYGVARKAVDKSGAEMLDMIGICVGIAGQVDVERGILVGTANLSRSVVDLPMAEL
                     LEKELGIPAIIRNDVQIAAAGENAFGAGKDVDDFVVVFVGTGIGGGIVRDGELIAGAA
                     GNAGELGHTTIDANGRICGCGGRGHLEAYASRTGMTRAIWGEVRRGRESVITELLPNV
                     HPADPAVQPIKSSIVRKALERDDAVVREIVEEGGRYLGLGLANVVNFLNPTRIILGGG
                     VVESLDLFFDMAERETRKQALAHAGEAVEIVRAGLGDFSGVVGAAVLAAEAQTS"
     gene            8762..9757
                     /locus_tag="VGR22_07390"
     CDS             8762..9757
                     /locus_tag="VGR22_07390"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEV2128423.1"
                     /translation="MVDEILAGRTNRPEEVAAFVDFENIRYSTINSYGQEPDPVAWRD
                     KALKYGLMSVAKAYADFDQHPPQVRTRLDVAGFEMHHYPVKRSTDSQGREKLASRSDL
                     NLVVDIINTALVRPDIKTFLLFSGDKDFIRLVTTLRNRLGLRVIICGVPGSVSPDLVA
                     AAGEEDPLVIEQGEDVDVAVIQAIDDYVQQLHDGFVPTQSHMSRSLWRFVDRSKLPSE
                     HIEAKVMEFLRKRVLVKRQTINGQGNELVTTELNMEHPLVREALGLDEEHNEDEEYWD
                     DEDGAFDDDSVEDHDAQDGDNAVWEENGEADADELVELQPPDLKNRSARPIWERR"
BASE COUNT         1677 a         3223 c         3349 g         1813 t
ORIGIN      
        1 ggtgaatgct ccgttcgcat atccgactag ccaggggccg tccgccgacc acgtcatggg
       61 aactgactcg cttgggcagg acctgctggc gcgcatcttc tatggcggcc ggatctcgct
      121 gtcggtgggc atcgttgccg ctctggtggc ggtcaccgtc ggtaccgcga tcggcgccgc
      181 cgccggcttc ttcggtggcg tcaccgacgg cttgctgatg cgctttactg acatgttcat
      241 cagcctgccg cagctgccgc tgctgctgct ggtcaccttc ctgttcaggg actcaatggt
      301 ctcgttcttc gacgatcagt tcggcacggg caatttcggg gtctttttcc tgatcgtgct
      361 ggtgatcggc ctgctcgcgt ggatgccgac ggcgcgactg gtgcgcgcca gcttcctctc
      421 tgtgaaggag aaggagttcg tcgaggcagc gacatcgatc ggcgcgacgc agcgctcgct
      481 gatcgccagg cacatcctgc cgaacgtgct cagcccgatc atcgtcgccg ccaccattca
      541 ggtcggtgcg gcgatcatca ccgagtcggc gctctcgttc ctcgggctcg ggttcccgtc
      601 ggatgtcccg acctggggcc agatgctctt ccaagccaag gactacctgg gcattaacat
      661 gtgggaggcg atcttccccg gcacgatgat cttcctcgcc gtgctggcga tcaactatat
      721 gggtgacggc ctgcgtgacg cgctcgatcc gcgcaagacg agctagcgtg ccgtcgacca
      781 caattgagcc tgatgctgca tggggaggcc acattgcgtg gcctccggcc tttcgacggc
      841 atgaagagga gcgacgagat gagcgcagcg acgcacgcgc agcgcgaggg atccttcgtc
      901 tggaacgacc acggacggtt cgtcgaggta catccggtcg ataccggctg gctggtcgtc
      961 tggggccact acgaggacat gggcgcggtc tgcaagaacg aaggcagcgt cacctatgcg
     1021 acgctcgcgg gtgcccggcg acgcctcgcg gatgccgccc ttgagctgac ccgcagcccc
     1081 caggcagctg ccagcgcggt ccagctgttc ggcttccaca cctttccgga gcacactggc
     1141 acccttcccg agccgctata ggccgcctgg gagctgaatc gaattgagag ttcgttgccc
     1201 ttatccgcat gtggtcgggg cttgtcatgc atgccagtgc cgggccatcg atgctgatgc
     1261 ctgtgtccgg aagacaacaa cgaccccgca cacgtcgtgc gggggtgcat cacacgattc
     1321 cggttggcgc tcggggccct atggctcccc ggagtcggcg agcaacccgc gacgggccgc
     1381 gatcgccacc gcctcgctgc gactggacgc atccagcttg ctcagcaccg agctgatgtg
     1441 gaacttgacg gtgtggtcgg tgatgcccag cacccgcgcg atcgccttgt tcggcaaccc
     1501 gttggcgacc tgcgtcagca cttcctgctc gcgcggcgag aggaccggca gatcgtcgag
     1561 cagcggggcg tcatcctcga cgtcatgctc ggtctggtcg acgacgtggg cgaagtcggg
     1621 gtgaatcacg ttgagtccgg tctctgcggc ccgcagcgtc cgctcgatct gctccagtgt
     1681 cgactgcggt gggagcatcc cgcccgggag cgtcgctgtg gcgaggcgct gcgcgatcgg
     1741 cggctcgccc agccagatca gcggcaggtc gtgctgctgc gccaccgcga ccagctctcc
     1801 aagcgtggcc ctcggcagat cgcccggctc gacgatgagc aggttgcacg gcggcaccat
     1861 ttcgccggcg atggcggggc ggggcatgat gacatcgcgg gcagacatcg ctaccgtgga
     1921 atagccgggg atcggttcga ggcgcgtgga gaggcgcagg ccctgatagc ggtaggccga
     1981 gaccagcccg atcaccggca ccttgccctg ctgacgcacc tgggggatcg gctcaccgct
     2041 gctggagatc gctcgcgtga acgcgagtgc cgaagatggc gattgtcgcg aggtcatgcc
     2101 gtccctccct cggtcgatct gccgccgcgt gcccggtgct acccggcacg gctgtagggt
     2161 ctagggtcgc ctccgaagat ggacgtgcac gccgcccatc tgaatctgcc ggagtggcgg
     2221 ttgtcgcata tctgtactca ggatagcact gcagggacat ttcgggagtg cgcccgtccg
     2281 tccgctgtaa tcatcgtacg cacgaaccac accagcggtt tcaccgctct gaacgggcga
     2341 cgccgtatac tcaggggcga aaaccgccgg gacacgctgc cacccgcctg ctaccaccgg
     2401 actatggaca acacgcatgc cgctaccaac aaccctcgac cgatccgaac ccgacaccac
     2461 cacgccggtc atcgaggtcc gcaacctgcg ccgatcgttc ggctcctttc tcgcccttga
     2521 cgacatcacc atgaccgtcc cgagagcctc cgtggggctc ctcggcgcga acggcgccgg
     2581 caagacaaca ctcatccgca cgctgctcgg cctgctcgcc ccggatcgtg gcgaggcgac
     2641 cgtggtcggg tacgacatac ggagtgaggg cacgagcctg cgcgcgtacg tcggctatat
     2701 gccggaggcg gaggggcttc cctccaacac caccgccgcc gacttcgtcg gtcacatggc
     2761 ggagatgagc ggcatcccgc cgcgcgccgc ccggcagcgc gcggccgatg tgctctacca
     2821 ggtcggcctc gccgaggagc gctaccggct catcaagggc ttctccaccg ggatgcggca
     2881 gcgggtaaag ctcgcccagg cgatcgtgca tgacccgcag ctggtcttcc tcgacgagcc
     2941 gaccgccggt ctcgatccgc gcggccggga ggagatgctc gagctgatca cgcgtatcta
     3001 ccgccagctc gggatcgccg tggtggtctc cacccacatc ctcgaggacg tcgaaaaggt
     3061 ttgcgactat gtgctgatcc tcgatgccgg gcgtctcgtc gctgcgcaac cgctcggcgc
     3121 gatggaggtg atcggcggcg aagtgaccgt acgcatcgat ggcgaccagg cagccttccg
     3181 cagcggactg gaggcacagg gggtcagcgt ccacgacggc ggcccggaga caccggacga
     3241 gctgtcggtc gacgtgcacg acgaggcggt ctacgacgtg atccgggacc tcgccgtcca
     3301 gcagggcgct gcgatccgtt cgatgcgttc aatgtcccgc tcactggagg acatctacct
     3361 cggcaatgtc ggtgcgtcgc gcgaggaggt gggcgccggt gtctgaacac gccaacgggg
     3421 tggcccccgg ccgctacggc gaggtcttcg atcgggggta cgcccactac gacgggccgc
     3481 gtgaggggcg tcgccgcgcc gtcaagtcgc tgttcgtcta ctcgatcaag cgggcgatgg
     3541 gcatccgcaa gtcatggacg gccaaggtcc tgccggtcct gctctacgtc tcctcgttcg
     3601 tgccgctgat cgtgatgatc ggcatcgcgg cgctgctccc ggatgcgaac gtcgcctcgt
     3661 atccggacta cttcgacggc attttcttcc tcgtcgggat cttcgcgggg acggtcattc
     3721 ccgagatcct catacccgac cggcaggaga agacgctgcc gctctatttc gcccgggcga
     3781 tcacccgctt cgactacgtg atcgccaaac tcgccgcagc ggctgtcctg accatgacga
     3841 tctcggtact gccggcgatc atcctctggc ttggccggca actgaccgcc gaatcgccgt
     3901 ggagcgcgat gcgggacaac atcggcgatc tggggcaggt gatcgtgctg ggcacgctga
     3961 tcgcgctcac gctcggcacg atcgcgctgg tgatctccgc gatgaccgac cgcaaggggg
     4021 tggcgatcgc cgtgattgtg atcgccttcc tgatcgtctc gggcctggtc gagctcgccc
     4081 tcaccgaact tgaccaggac tggcgacgct acctcgcctt tggccacatg cagctcgtgt
     4141 tcagtgcgat cagtgcgtcg ctgttcaacg cggagccgaa catggatgtg caggaggcgg
     4201 acctgccgac ctggatgtac tacgcttgga tctgcttcat tatcatcgca gggtcgctct
     4261 tcgtgcgctg gcgctactca ccggggaaca cggcatgatc ggcagacgac gagtgacgac
     4321 acccgagcct gatggccagg ccgacgagcc acacgtcatc ccggggctcc aaccggggca
     4381 ggagcccacg gtcctggtga gtgaggtctc gaagtggtat ggcgacatcg tcgccgtttc
     4441 caacgtcacc ttcggcgtgg cgccgggcgt caccggcctc ctcgggccca acggcgcggg
     4501 caagtcgacg ctgctgaaga tgatctgtgg cttgcagtcg acctccgcag gcgagattcg
     4561 catcgcgggg cagccggtgc ggggccgccc ggtggtctac cggaaactgg gacttgtctc
     4621 ggagcaggag acgatctacc cgtttctttc tggccgcgag tttgtcaccc tcaatgcgcg
     4681 tctccagaag gtgccgaaca tcggtgatgc ggtcgagcgc gcgatcgacc tggtggagat
     4741 gggcccggcg gccgagcgca aggtcggcgg ctactccaag ggcatgcggc agcggatcaa
     4801 ggtcgccggt gcgctggtcc acgacccgga catcatcctg atggacgagc cgctcaacgg
     4861 caccgacccc gtccagcgcg tccatctgat tcgcctgatt cgcgatctcg gccgatccgg
     4921 caagacggtg atcgtctcgt cgcatgtgct ggaggaggtc gaacgcttcg ccgaaaacat
     4981 catcgtgatt gtcaacggca ggctcgccgc cgtcggcgac ttccgggcga ttcgcgagcg
     5041 catcgattcc catgaccgcg agattcgcct gcgcgccgac cgcccccggg agctcgccag
     5101 cgcgctcatc ggcagcgagg cggtgcgggc ggtgcggatc gacgagcagg ggcggatcct
     5161 tgccgacacg cgcgacgtgc gcgcctttta caaggtagtg ccggcgctgg cccagcggtc
     5221 cggggtgcgc ctctacgaga tccaggcgct tgacgagtcg ctggcgtcgg tcttcgccga
     5281 cctcgtggag ggctgacgga tgggtgcgcc ggtaaccggc gtgcatccct tcgcttgcta
     5341 ttttgagcga aacccgaacg cagcgagggt gcagtcgcga aatctcttgc gtgctgttcc
     5401 tcaggacacg tgcaggggcg agatttctcc actccgcgac gaagccgcgg agcctgccct
     5461 gagcgagggc cgccggtccg agtcgaatgg ggtcgaaata acacaacacg tgcatgcaca
     5521 catcctgaag cgagcctcga tggttcgcac aaccgatctg gaggccggat gaccggcacg
     5581 ctgacgatca tgcagctcac catccaccag ttcctgcggg ccaaatcggt gctggtagtc
     5641 gccgccatct gtgcgatccc gatttttctc gcgctgatcc tgcgcatggc caatccgaat
     5701 ctcagtgtcg tggacatgcg agagaccatc ggcgagggta tcttccttgg cctcttcagc
     5761 tccacgctgc tgccgctggc cgccctcgtg ctctctaccg ccgcgctggg cgatgagctc
     5821 gacgacaaga cgctccacta cctggcgctc aagcccgtca gtcgcctgcg catcgtgatc
     5881 gagaagtaca tcggcacgct gctgatcacc gtaccgatcg cctggggtgc gctgctggcc
     5941 gtctggctcg tggtctcgtg gggtgagatc gacgcaactc gtgacctgcc atggccgatg
     6001 ctggttgcca gcatcggcgg ggtggccggc tttgccgcac tctttcagct tgtcagcctc
     6061 tacgtgcccc gggcgctgct ggtcggcatt ttctatgtct tcgtctggga ggcgaccgtc
     6121 tcgcagttcc tgccgggcat ccggaccatc agcatccgcc attacatgca gtcgatcttc
     6181 gtccggctcg cgggcgaccc ggagatcacg ctggatggtg tctcgcgcct gtccacggca
     6241 tcgatcacca tcgtggtgat catcgtgctc tcgctgctgc tctcggcccg gcggttgcga
     6301 cggatgaacc tcgactgagc cttgtcgcca cggcgcgcag cgtgcgatgg tgcccgcatt
     6361 gagtccggcc ggtgagggga aaggtgcacc atgatttcac acgacttgat ggcagtgaac
     6421 cactgggtag ccgattcccg gccgatgggg atcggactga tgatgccgat ttccgagggc
     6481 gcggcgttcg gcgatacgcc ccatttctcc gatatcatcg agatggcaga ggttgcccgg
     6541 gacgtcgggg tcgacacgct ctggttcgcc gatcacttca cgttcggcac gccggaggag
     6601 gggcagcggg gcgtctggga ggcgttcacg ctgatggcag gtgtagccgc gcgcgtgccc
     6661 gatctcaaca tcggctcgct cgtctcctgt accggcttcc gcaacccggg cgtgatcgcc
     6721 aagatgaccg agacaattga cgatatcagc aatgggcggt tcatcctggg gctgggcgcc
     6781 ggctggcaca agccggagta cgaccagttc gggatgccgt tcgaccaccg cgtcacccgc
     6841 ttcgaggagg cgatcgagat tatccacccg ctgctgcggc ggggcaaggc gagctccgag
     6901 ggacgcttct actcggcgca gggcgccgtc aaccagccgc gcggcccgcg cccgcagggc
     6961 gcgccgattc tcgtcggctc caccggtgac cgcatgctag gcatcaccgt gaagtacgcg
     7021 gacgcctgga acaccgtctg gcacaaggag gccgaggagg tgcgaccgct gatggacaag
     7081 gtcgaccagg cctgccggca cgccggacgc gacccgcaga cgctggtccg ggcggtgggc
     7141 ggcaatgtcg cgatggaggg ctacctcggt cgccgtccga atcccatcga gggtgacgat
     7201 gaggcgaagg cggaggcgat tgccggattc cgcgaggtcg gcatgaggca cttcgtcgcc
     7261 ggactggatc cctcgacgcc acagtcgatc gagcaattcg gccgcgtgat cgagatcctg
     7321 gacagaagct agcgcccggg aatccgtagc gccggcttcg taccggccag cgggccgccg
     7381 gagtcggtcc tcaccgcaaa tggagccgca cggaggggca cagatgggac ggttggcgat
     7441 cggcgtcgat ttcggaggca ccaaggtgct cgccagtgtg gtggatgtcg ataccggcaa
     7501 gatcaagggc actggcaaga agaagaccag cgccaccgac ggccctgatg agctgatgga
     7561 ccgcatctac ggcgtcgccc ggaaggcggt cgacaagtcc ggcgcggaga tgctggacat
     7621 gatcggaatc tgcgtcggca ttgccgggca ggtggatgtc gagcgcggca ttctcgtcgg
     7681 tacggccaac ctcagccgat cggtagtcga cctgccgatg gcggagttgc tggagaagga
     7741 gctcggcatc ccggcgatca tccgcaacga cgtgcagatc gccgccgccg gtgagaacgc
     7801 gttcggcgcc ggcaaggacg tcgacgactt cgtcgtcgtc ttcgtcggca cggggatcgg
     7861 tggtggcatc gtccgggacg gcgagctgat tgccggggcg gctggcaacg ccggggagct
     7921 gggccatacc accatcgacg ccaacgggcg tatctgcggc tgcgggggcc gtgggcacct
     7981 cgaggcctac gcctcgcgaa cgggcatgac gcgcgccatt tggggtgagg tgcgacgcgg
     8041 acgcgagtcc gtcatcaccg agctgttgcc aaatgtccac ccggccgacc ccgctgtcca
     8101 gccaatcaag tcgagcatcg tgcgcaaggc gctcgagcgg gatgatgccg tcgtccgcga
     8161 gatcgtcgag gagggaggcc gctacctcgg ccttgggctg gcgaatgtgg tcaacttcct
     8221 caatcccaca cgcatcatcc tcggcggggg tgtcgtggag tcactcgatc tgttcttcga
     8281 catggccgag cgcgagaccc gcaagcaggc gctggcccac gccggcgagg cggtcgagat
     8341 cgtccgggcc gggctcgggg acttctccgg ggtggtcggg gcggccgtgc tcgcggccga
     8401 ggcgcagact tcctgatcac gccggaataa actggctcgt ggtggtggtc ctgggtagcc
     8461 atggcacata gacgatcatg tacgggatca ctatcattga ctcgtcacat attgtggacg
     8521 ctggggcggt cgatggtgtg tgaggagagc ggctggccca aatcgtgctg atatgtgtaa
     8581 gtggactgtt cgttggtgta gatgcatcgc aagcgggcat tgagagaagg gatcgcactc
     8641 actgggtgcc accgcagccg ggcagcaggt ccgggcgtcg cgagcgatcc tgtgtgagct
     8701 gacgtcagat acgttcgtca ttcccgtatt ccaaaatgct ttgtgaagag gagaccccct
     8761 catggtggat gagattcttg ccgggcgcac gaatcgtcca gaagaagtag ccgcattcgt
     8821 ggatttcgag aacattcgct attccacaat caattcctat gggcaggagc cggaccccgt
     8881 cgcctggcgg gacaaggcct tgaagtacgg gttgatgtcg gtcgccaagg cttacgccga
     8941 tttcgaccag catcccccgc aggtacgtac ccgcctcgat gtcgccgggt tcgaaatgca
     9001 tcactatccg gtcaagcgct ccaccgactc gcagggacgc gagaaactcg cctcccgctc
     9061 cgatctcaac ctggtcgtcg acatcatcaa tacggccctc gtgcgtcctg acatcaagac
     9121 cttcctgctt ttcagcggtg acaaggactt catccggttg gtgaccacgc ttcgcaaccg
     9181 gcttggcctg cgcgtgatca tctgcggcgt gcccggctcg gtgagcccgg atctggttgc
     9241 cgccgccggg gaggaggatc cgctcgtcat tgagcagggc gaggatgtgg atgtcgccgt
     9301 catccaggcg atcgacgact acgtccagca gctgcacgac ggcttcgttc ccacccagag
     9361 ccacatgagc cgctcgctgt ggcgcttcgt cgaccgctcc aaactaccgt ccgaacacat
     9421 cgaggcgaag gtcatggagt tcctgcgcaa gcgggtgctc gtcaagcgcc agacaatcaa
     9481 cgggcagggc aacgagctgg tcaccaccga gctcaacatg gagcacccgc tggtgcgcga
     9541 ggcgctcggt ctcgacgagg aacacaacga ggacgaggag tactgggacg acgaggacgg
     9601 ggccttcgat gacgatagcg tcgaggacca cgacgcgcag gacggggaca acgctgtctg
     9661 ggaggagaac ggcgaagccg acgcggacga gcttgtcgag ctgcagccgc ccgacctcaa
     9721 gaaccgcagc gcccgcccca tctgggagcg ccgctaaccg gttctgatcg tttgatgcgt
     9781 cgagtcgaac agcatatggt ggtggtcccg ccctgtaaat ggcggggttc gtcgtggata
     9841 ccaggcacca ctgttgacgc ctgctgctgg ttcatggaat ctctgctgtc cgggacgtct
     9901 gtacctctga gcaggcctgg tagctactcc agggcggcgg cgatcaggtc gtcggcgatc
     9961 ttcgccgagg gcagcacgcc gggcactccg gcacccggat gcgtccctgc tccgacgaag
    10021 tagaggttct cgatgtcgtc ggcccgattg tgcggccgga ac
//
LOCUS       DASYWL010000210         3515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_385192, whole genome shotgun sequence.
ACCESSION   DASYWL010000210 DASYWL010000000
VERSION     DASYWL010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385192"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VGR22_07395"
     CDS             <1..284
                     /locus_tag="VGR22_07395"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEV2128424.1"
                     /translation="VSVHVVAGDVYSGIEGEAYDLIVSNPPFHQGKMINYDMPQRLIG
                     EASEHLKPGGRLVIVANAFLPYDRVMRAHFAEVDVVAATRQYRVLATST"
     gene            complement(323..826)
                     /locus_tag="VGR22_07400"
     CDS             complement(323..826)
                     /locus_tag="VGR22_07400"
                     /inference="COORDINATES: protein motif:HMM:NF020177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthrone oxygenase family protein"
                     /protein_id="HEV2128425.1"
                     /translation="MDLTTIDGWASLTLFTATVVTGLVAGIYLAFSIAVIPGLRVSGD
                     ATFIETMQNVNTKILNPAFLVIVFGSAVLPIIAAVLLIIDRDEGARWWAAGGAMLALT
                     AFVTTVNRNIPLSRALAAAGPVTEIADQRAVRQAFEERWVRWNHHRSLASSFALVALV
                     AAIFGRG"
     gene            1015..1197
                     /locus_tag="VGR22_07405"
     CDS             1015..1197
                     /locus_tag="VGR22_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128426.1"
                     /translation="MLIVVVLVSDSLYALASGSVGPWLRRHPGSSRHSDRISGLVYRA
                     LGLLVALTGSGSAKGA"
     gene            complement(1226..1816)
                     /locus_tag="VGR22_07410"
     CDS             complement(1226..1816)
                     /locus_tag="VGR22_07410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEV2128427.1"
                     /translation="MKSVCVFCASSVGTNPAFIDTTRMLGEAVAARGLRLVYGGGRVG
                     LMGVLADTVLAAGGDVTGVIPRSLEDREIAHHGLTELHVTESMHARKALMEQLADGFV
                     ALPGGFGTLDEFCEIVTWAQLGYHTKPIGLVDVNGYFSALRTFFDRAVEAGFVRPDHR
                     QMLLEAETPDALLNAMAAWRPTGPPKWTRTTTPATD"
     gene            1960..2643
                     /locus_tag="VGR22_07415"
     CDS             1960..2643
                     /locus_tag="VGR22_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEV2128428.1"
                     /translation="MRRLLRPRQSLFRDRIDAGEQLAEQLTTWAGHDPLVLGLPRGGV
                     PVAAEVARRLGAELDVLVARKVGAPRQPELAMGAVTADGGVYLDTHIVDHLRVPADTL
                     ERLIEQEREVARRRDQRFRGDRPFPRVKGRTVIVVDDGLATGATMRAAVRVLRKRDPR
                     RLIVTVPVGSTQACADLAEEADEVVCLHSFDSFQAVGLYYEDFRPTEDAEVEELLARF
                     RGAAEPDRS"
     gene            complement(2638..3153)
                     /locus_tag="VGR22_07420"
     CDS             complement(2638..3153)
                     /locus_tag="VGR22_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128429.1"
                     /translation="MTDTAPDRLHLEDDGTFPNNGLPVLLWRSAILTNGDTASAIEDR
                     FHANGWGGSWRNGIFTYHHYHSTAAEVLGIARGSVTIQLGGPSGETLDLAAGDIALLP
                     AGTAHKNLGQSDDLLVIGAYPPGQRPDMLRGEPDERPEADQNIATVPLPATDPVTGDD
                     GPPPEWRRARS"
     gene            3170..3304
                     /locus_tag="VGR22_07425"
     CDS             3170..3304
                     /locus_tag="VGR22_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128430.1"
                     /translation="MTRIVSRALPQHIGHDENPGVSARVYTLTAALAALGADVARLSA
                     "
BASE COUNT          569 a         1100 c         1248 g          598 t
ORIGIN      
        1 gggtatcggt ccacgtggtg gcgggagacg tgtacagcgg catcgagggc gaggcatacg
       61 acctgatcgt cagcaacccg ccgttccacc aaggcaagat gatcaattac gacatgccgc
      121 aacgactgat cggcgaggcg agcgagcacc tgaagcccgg cggaaggctg gtgatcgtcg
      181 ccaacgcctt cctgccatac gaccgggtga tgcgggcgca cttcgccgag gtggatgtgg
      241 tggctgccac ccgccagtac cgcgtcctcg ccacctcaac gtagatgtgg atcctgatat
      301 ccgggaacag agggaagggc gcctaccctc gtccgaagat ggcagcgacg agggcgacga
      361 gtgcaaagga ggaggcgagg gagcggtggt gattccagcg cacccatcgc tcctcgaatg
      421 cctggcgcac cgctctctgg tcagcgattt cggtgaccgg ccctgccgcg gcgagggccc
      481 gactcagcgg gatgttgcgg ttgacggtcg tcacgaatgc ggtcagcgcc agcatcgccc
      541 cgccggcagc ccaccagcga gcgccctcgt cgcggtcgat gatgagcagg accgccgcga
      601 tgatcggcaa taccgcacta ccgaacacga tgacgagaaa cgcggggttc aggatcttcg
      661 tgttgacgtt ctgcatcgtc tcgatgaagg tggcatcgcc gctgacacgc agccccggta
      721 tcactgcgat agaaaaggca aggtagattc cggcgaccag cccggtcacc actgttgcag
      781 tgaagagggt cagtgaggcc catccatcga ttgtcgtcag gtccatgatc gctccgcgtg
      841 gtatccgtca gtggtcaggc tcaaggccaa gcataccggg gagatgtggc aggcgtcgcg
      901 cccggtgttg ggaggcacag atggggacga cgtgacgtgt gttccgagtg caggacaggg
      961 cacaatgtgg gctgatgcgt cccgcgtcta accgggcggt gccccggcgt cgcagtgctg
     1021 atcgtcgtgg tgctggtcag cgactcgctc tacgcgcttg ccagtggttc ggtggggccc
     1081 tggctgcgga ggcacccagg gtcgagccgg cacagcgatc gcatctcagg cctcgtctac
     1141 cgggcgctcg gtctcctcgt cgcactgacc gggtccggct ccgccaaggg cgcctgaggc
     1201 gccgtccatc cgctcacggt gcggatcagt cggtggcggg agtggtcgtc ctggtccact
     1261 tcggtggccc ggtcggacgc caggcggcca tcgcattgag cagcgcgtcc ggcgtctccg
     1321 cctcgagcag catctgtcgg tgatccggac gaacgaaccc tgcctcgacg gcacggtcga
     1381 aaaacgtgcg cagcgcggag aagtagccgt tgacatccac gaggccaatc ggctttgtgt
     1441 gatacccgag ctgcgcccag gtgacgatct cgcagaactc gtccagcgtg ccgaacccac
     1501 ctggcagcgc cacgaatccg tccgcgagct gctccatcag tgccttgcgg gcgtgcatcg
     1561 actcggtgac gtggagctcg gtgagcccgt ggtgcgcgat ttccctgtcc tccagtgagc
     1621 gcgggatcac gccggttacg tcaccgccgg cggccagcac cgtgtcggcg agcacgccca
     1681 tcagcccgac ccggccgccg ccgtagacga ggcgcagacc gcgcgcggcg accgcctcgc
     1741 caagcattcg cgtcgtgtcg atgaaggcgg ggttggttcc gacgctcgat gcacaaaaga
     1801 cgcagaccga tttcacagtg ctctccttgg acgtgacgtc gacagtctcc ctcgtcgcaa
     1861 gtgtcgccgg agccgcacgg tggtgccaat cctgcggcgg tactggtgca tgaatgcaga
     1921 cgcaggtgat cggcttggca gggtgaagcg gggagcgcga tgcgcaggtt gctcaggcca
     1981 cgacaatcgc tgtttcgcga ccgaatcgac gccggtgagc agctcgccga gcagctgacg
     2041 acctgggccg ggcatgatcc cctcgtgctg ggtttgccgc gtggcggcgt gccggtggcg
     2101 gcagaggtcg cccgcaggct cggggccgag ctggatgtcc tggtggcacg gaaagtcggc
     2161 gcgccacggc agccggagct ggcgatgggt gcggtcacgg cggatggcgg tgtctacctc
     2221 gacacgcaca tcgtggacca tttgcgggtg ccggctgaca cgctggagcg gctcatcgag
     2281 caggaacgcg aggtcgcccg gcgccgggac cagcgcttcc ggggcgaccg gcccttcccg
     2341 agagtcaagg ggcggacggt gatcgttgtc gatgacgggc ttgccaccgg cgcgacgatg
     2401 cgcgctgccg ttcgtgtcct tcgcaagcgc gaccctcgac ggctgattgt cacggtgccg
     2461 gtcgggtcga cacaggcgtg cgctgacctg gccgaggagg cggacgaggt ggtgtgcctg
     2521 cattccttcg attcgtttca ggcggtcggg ctctactacg aggatttcag gccaacggag
     2581 gatgccgagg tggaggagct gctcgcccga ttccgtggcg cagccgagcc ggatcgatca
     2641 tgaccgcgcc ctccgccact ccggcggtgg accatcatcg cccgtgaccg gatcggtggc
     2701 aggcaggggc acggtggcga tgttctgatc cgcctcaggc cgctcgtccg gctcaccgcg
     2761 cagcatgtct ggtcgttggc cgggtggata ggcgccgatc acgagcaggt cgtccgactg
     2821 cccgaggttc ttgtgcgcgg tgccggctgg gagcagggcg atgtctccgg cagcgaggtc
     2881 gagggtctcg ccgctcgggc cgccgagctg gatggtgacc gacccgcggg cgatgccgag
     2941 cacctccgcg gccgtcgagt ggtagtggtg gtacgtgaag atgccgttcc gccaggagcc
     3001 accccagccg ttggcatgga agcgatcttc gatggcggag gcggtgtcgc cgttcgtcaa
     3061 gatcgcgctg cgccagagga gtaccggcag cccgttgttc gggaaggtgc catcgtcttc
     3121 gaggtggagg cgatcggggg ccgtatcggt catgatggtg gttccctcgg tgacacgaat
     3181 cgtctcgcgg gcgttgccgc aacatatcgg ccacgacgaa aacccgggcg tctcagcccg
     3241 ggtctacacg ttgacagccg cgctcgctgc ccttggtgcc gacgtcgcgc gcctcagcgc
     3301 gtagacctgc acttcgatgt gcgggtttgt ggacaggaac cagagttcgg tccttgtcat
     3361 cgcgcaacca ccgtcacgct gagcccgtcg gtgcgtcccc gcgcgaagcg aatcgtgatg
     3421 ggagacaggg ctcgcgcgct ggtcataccg gtcggcaaga agacgagatc gccattgcct
     3481 ctgggcgatg gcgggggatc cttcggctcc ggcct
//
LOCUS       DASYWL010000211         9438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_497449, whole genome shotgun sequence.
ACCESSION   DASYWL010000211 DASYWL010000000
VERSION     DASYWL010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9438
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497449"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VGR22_07430"
     CDS             <1..439
                     /locus_tag="VGR22_07430"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2128431.1"
                     /translation="VQAIDRLASRDAGAHGLWGLLEFENGTVGTIETVWNLPAGAEIA
                     TDDALNVIGTDGVARTQLDPAPLRIWTGEGSGFPDVTYGPEVHGAVTGALGTELAHFA
                     QCALAGTPSPIVTPEDGALALAVVLALIEAAGTGEAITRVVRS"
     gene            complement(551..1933)
                     /locus_tag="VGR22_07435"
     CDS             complement(551..1933)
                     /locus_tag="VGR22_07435"
                     /inference="COORDINATES: protein motif:HMM:NF006579.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV2128432.1"
                     /translation="MIQTKLEAVLQAIDSNADVDRGDLFTLVRQPSISAQNVGVRECA
                     MLVRDYMERAGLSTRLLETSGHPMVLGTWLEAGPDKPTILFYGHYDVQPPEPLELWES
                     EPFEPVVRNGRIYARGVADNKAQFFSHLKAIRHWMEQTGELPVNVKFLAEGEEEVGSP
                     HLAEIVAQYTDDLRADIVYTSDGPVQDTAYPEICFGVRGMLYIELRATGPSRDVHSGH
                     WGGVAPNPAWMLVDALRTMRDEAGNITIDGFFDEVKDPTPAARAAMDNLPFDVQEMLT
                     RVGLDAMVPPAELPWADRIMARPTLNIAGFTSGYGGPGSKTIIPSKATVKIDMRLVPD
                     QTPDEIWEKVKAHVEKHAPSVEAVRLDGGMVPSYTPVEHPMADLVRAAVERGFGTRPV
                     DVPLVGGSLPDAVWTKTLGTPSFLVPYGDPEQANHAPNESYAVDRFHQGIRTSAILLA
                     ELARLEPDAP"
     gene            2104..3567
                     /locus_tag="VGR22_07440"
     CDS             2104..3567
                     /locus_tag="VGR22_07440"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEV2128433.1"
                     /translation="MRLLLAPVRWLGRFSVFHSLRFRLTLLVLLGSLPALGLLLLTAN
                     QQRDDALAEGQETANRLVRLAAADQRRIFEQAQQLLATNVRLEEVRGNDAEACSELMA
                     ELLAINEEFDNIGVVTEDGTVFCSGPGGDLSGILGDPSFVEEAFETDELLIGIQEAGP
                     FVGEPSVTFAAPVPPGAEDPRRIVFASLNLGALDTFATIANLPEGTVFSVYSEEGRLL
                     LRSPQDPDAIGPVFDDRVVEVMVEDPNGASVEDIEDEEYIYAGEPIVVRLADGSLVTA
                     GFVTVAVPKAATVARAADSFQDNLSRLGLAAMVAVALAWIGADLFMSRDAETRKSIIA
                     ELYRVYETGDLRRLDDIVAVDVVDRSPAPGQVQGLSGYKQLVTQFRAAFPNGRIVPDG
                     IMVDGDMVVARVTLSGTQVGEFFGIAPNGKPVTAKGVETFRFAHGSVVEMWSLFTPLV
                     VVKRPQDEPVALDEEPEPDRVGPLKRMAEFLRRRPTR"
     gene            3564..4634
                     /locus_tag="VGR22_07445"
     CDS             3564..4634
                     /locus_tag="VGR22_07445"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEV2128434.1"
                     /translation="MITVGSATEARLGALEHEVEGMIRPPDHFFDPEDTEDVSDLRLV
                     MQRLVRWMIEGYQRANATDLASAVAFQALVAVVPMFLLLISVAGLFLQDGRVLQQAIL
                     TIAWVLPSSASNDAFTAAFEARRNSGLFGALSLLGFAWVGTGFVSAMARSMNRIYGVP
                     NAGYLKEKQRGFIVILLFALFFLISVAASVVPTFFVANAGDLPVVFRRWVLASGQGQL
                     LSYALAVVAAYGLFVTIFRLVPNAKQRMPDVWPGAVISSLLFLLVTQAFPIYLRMVGG
                     ANRYGLVLGLISLMVIAFYALAHVILFGTYVNATWQRHRDAMRRQKRRDPRRDTTPRP
                     PGPAAAQDPQAVRADTMEHVIR"
     gene            complement(4836..6812)
                     /locus_tag="VGR22_07450"
     CDS             complement(4836..6812)
                     /locus_tag="VGR22_07450"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEV2128435.1"
                     /translation="MSANSAKRIIPYGAWPSPIIPEMLTAQIIRLGPPAADGATRYWL
                     EARPSEAGVNRLVRQRGDGKPEPITPLEINVRSAVHEYGGGAWAVRDGVVIVSNYADN
                     RLYRLTDGAEPASITPENPDWRYADLLLDPERGRIIAVREDHSMKDAEPVNTLVLLDV
                     DGPNEDGGRVLVSGSDFFASPALTADGQHLAWLAWNHPLMPWDGCELWTAELGGGTTL
                     RKLRHIAGDAQESIFQPRWADNGSLVFVSDRTGWWNLYRQDLSADDATALCLMEAEFG
                     VPQWVFGMSTWDFAPDGSIVCLWTQDGTWSIGRLPTDGGELRSYATPFTVIGDIHVQP
                     ETNQALMIASSPEAPKQVIALDIESGNDVTLRYSSEIELPEGTISVPEPISWPTTGGA
                     TAHGIFYPPASAEAAGPAGERPPLIVKSHGGPTSMTTADLDLGIQFWTSRGFAYLDVN
                     YGGSTGYGRAYRERLIGNWGIVDVEDCIAGAHWPAEQGRVDGNRMAIKGGSAGGYTTL
                     AALTFHDAFRSGVSSYGIGDLETLARDTHKFESHYLDSLVGPYPAEKARYEARSPIHH
                     VERLSSAMLLLQGLEDKVVPPDQAETMAQAVRDNGMPVALLMFEGEGHGFRRAETIHR
                     ALEAELSFHSQIFGFPHPEGITPLQIENMRPPGR"
     gene            6982..7950
                     /locus_tag="VGR22_07455"
     CDS             6982..7950
                     /locus_tag="VGR22_07455"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2128436.1"
                     /translation="MALVIDHVSKRFGGFLAIDDVSFTVQEGRMFGFLGTNGAGKTTA
                     MRMVLDILRPDSGTITWNGMSNRQIARQEFGYLPEERGLYPKMTVQEQLLFLAQLYGA
                     EKRAAQAALDEWLARLDIEENRHKTIEQLSKGNQQKIQFLAAVLHDPEILILDEPFSG
                     LDPVNIAQMEAAFNEMKARGKTIIFSTHQLSQAQRLCEDVAIIHRGRLLVAGDVEAVR
                     ESIDVKILRVGVAGDERLEWLDPYPEVEIRQQLRDHIEMQIPDEAVAQRILRDVLARG
                     ETVELFEVTVPSLNDVFLTRVRQSGATAEDIARIEASRSEHGEAVA"
     gene            7947..9251
                     /locus_tag="VGR22_07460"
     CDS             7947..9251
                     /locus_tag="VGR22_07460"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2128437.1"
                     /translation="MSVRPSRIRLVARREWLQKIRRRSFIIATIVQIVIVLGLILAPV
                     ALSAIFGGDEGTSTEEVLIVNRTDQPEIVADTVAFIAHTGLGDQIELRAAPGDTDAVR
                     AIEAGDADAVLEIERGADGALAFTYTDEDGATDQTAVVIQALAGELALRDQLVRIGVD
                     PEQAREVRSLPNMVMQAVGEAEADNAETEQGFRYGVAYVAALVMYMVVLIYGMWVAQG
                     GVEEKSSRIMEIMINAASPTELMYGKIIGIGLAGLTQILPTLAIAGIGLYSQEALADA
                     LDATGIHLSGIDFGAISVQLVGFFLIYFLLGFLLYAALYAGVGSLVSRQEDVQTVMAP
                     MTFTVVLGFFGALFTLGAPDSIVAQVVSIFPFTSPLAMVPRILLGDPELWEILLSIAL
                     LAVSGYLAVRLAARIYRMGVLMYGQKPSLKVIFRPGIITAAR"
BASE COUNT         1501 a         2948 c         3274 g         1715 t
ORIGIN      
        1 cgtgcaggca atcgatcgtc ttgcgagtcg ggacgccggc gctcacgggc tctggggcct
       61 gctggagttt gagaacggta cggtgggtac catcgagaca gtgtggaatc tgcccgccgg
      121 tgccgagatc gccaccgacg acgcgctgaa cgtcatcggc acggatggcg tggcgcggac
      181 acagctcgat ccggcgccgc tgcgcatctg gactggtgag ggcagtggct tccccgatgt
      241 gacctacggg ccggaggtgc acggggcggt gacgggagcg ctaggcaccg agctggcgca
      301 ctttgcccag tgcgccttgg cgggaacgcc gtcgccgatc gtgacgccgg aggatggcgc
      361 tttggcgctg gccgtcgtgc tcgccctgat cgaggcggcg ggaaccggcg aggcgatcac
      421 ccgggtggtg cggagctgac ggtcgctcac acacggagtg acgcagggac tgaccgccgt
      481 tgaaggtcag tccctgcagg attaagcgag tctcgctgcg ggccggcgat gccgttcggg
      541 ataccggttt ctacggggcg tcaggctcca agcgggccag ctcggcgagg aggatggcgc
      601 tggtgcggat gccctgatgg aagcggtcaa cggcgtagga ctcgttcggc gcgtggttgg
      661 cctgctccgg gtcgccatag ggcacgagga acgatggcgt gccaagtgtc ttcgtccaga
      721 cggcgtctgg caggctgccg ccgaccagcg gcacgtcaac cggtcgtgtg ccgaagccgc
      781 gctcgacggc cgcacgcacg aggtccgcca tcgggtgctc gaccggtgta taggatggca
      841 ccatgccccc gtccagccgc acggcctcga cgcttggcgc gtgcttctcg acgtgcgcct
      901 ttaccttctc ccagatctcg tccggcgtct gatccggcac gagccgcatg tcgattttca
      961 ccgtcgcctt cgacgggatg atcgtcttgg agccaggacc accgtagccg ctggtgaagc
     1021 ccgcaatgtt gagcgtcggg cgcgccatga tacggtctgc ccatggcagc tcggcgggtg
     1081 gcaccatcgc gtccaagcca acgcgggtga gcatctcctg cacgtcgaag ggaagattgt
     1141 ccatcgcggc gcgggcagcc ggggtcggat ccttcacctc gtcgaagaag ccatcaatcg
     1201 tgatgttgcc cgcctcgtca cgcatcgtcc ggagggcgtc gaccagcatc catgccgggt
     1261 tgggcgccac gccgccccag tgtcccgagt ggacatcgcg gctgggaccg gtcgcgcgca
     1321 gctcgatgta gagcatgccg cgcacaccga agcaaatctc ggggtacgca gtgtcctgca
     1381 ccgggccgtc ggaggtgtag acgatgtcgg ctcgcaggtc gtcggtatac tgggccacaa
     1441 tctcggccaa gtgcgggctg ccgacctctt cctcgccctc ggcgaggaac ttgacgttca
     1501 ccggcagctc gccggtctgc tccatccagt gccgaatcgc cttgaggtgg ctgaagaact
     1561 gtgccttgtt gtcggcgaca ccgcgtgcat agatgcgccc gttgcggaca actggctcga
     1621 atggctccga ctcccacagc tccagcggtt cgggcggctg cacgtcgtag tggccgtaga
     1681 agaggatggt tggcttgtcc ggcccggcct cgagccacgt accgagtacc atcgggtggc
     1741 cgctggtctc cagcaggcga gtgctgagac cagcacgctc catgtagtcg cgtaccagca
     1801 tcgcacactc gcgcacgccc acgttctggg cgctgatgct cggctggcgc acgagggtga
     1861 agaggtcacc ccggtcgaca tcggcattgc tgtcgattgc ctgcagcacg gcctccagct
     1921 tggtctgtat cattgaggga actccacctg gcttgcgtcg cgttcagtcg ctgcaggcat
     1981 tgtcacagag aatgcgattc atgagtactg ggtcccacag aaaaggcggg cgagcatgac
     2041 cattcctcga gatgaccggc agccacgacg ggtgggtagc gtgcgtccgg aagggaacag
     2101 cgcatgagat tgctgctcgc gccggtccgc tggctcggtc gattctcggt cttccacagt
     2161 ctccggttcc ggctgacgct gctggtgctg ctcggttcgc tgccggcgct tggtctgctg
     2221 ctcctcaccg ccaaccagca gcgcgatgac gctctcgccg aggggcagga gacggcgaac
     2281 cggctcgttc gcctcgccgc ggccgatcag cgcaggatct tcgagcaagc ccagcagctg
     2341 ctggcgacca acgtgcggct ggaggaagtt cgcggtaatg acgccgaggc ctgctctgaa
     2401 ctcatggcgg agctgctcgc catcaacgag gagttcgaca acatcggggt cgtgaccgag
     2461 gacggcacgg ttttctgctc tggccccggg ggcgatctca gcggcatcct cggcgacccg
     2521 tcgttcgtcg aggaagcgtt cgagaccgac gaactgctga tcggtatcca ggaggcgggg
     2581 ccgtttgtgg gtgagccgag cgtgacgttc gccgcaccgg tgccgccggg ggccgaggac
     2641 ccgagacgca tcgtcttcgc ctcgctcaat ctcggtgcgc tcgacacctt cgccaccatc
     2701 gccaatctgc cggaagggac ggttttcagc gtctacagtg aagaaggcag gttgctcttg
     2761 cgctctcctc aggatccgga cgcgatcggc ccggtgttcg acgatcgggt cgtggaggtc
     2821 atggtcgagg atccgaatgg cgcatcggtc gaagatatcg aagatgagga gtacatctac
     2881 gccggtgagc cgattgtggt gcggctcgcc gatggaagcc tcgtcacagc gggattcgtc
     2941 acggtggcgg tcccgaaggc ggcaaccgtt gctcgggcag ccgactcatt ccaggacaac
     3001 ctcagccgac tcgggctcgc cgcaatggtc gcggtggcgc tcgcgtggat cggcgccgac
     3061 ctgttcatga gtcgagacgc cgagacgcgc aagtcgatca tcgccgaact ctatcgtgtc
     3121 tatgaaaccg gcgatctgcg acgcctggac gacatcgttg ccgtggacgt ggtcgatcgg
     3181 tcgcccgctc caggccaagt tcagggactc tccggctaca agcaactggt gacacagttc
     3241 cgcgccgctt tcccgaacgg caggatcgtc ccggacggta tcatggtgga cggggatatg
     3301 gtggtggcac gcgtgacgct atcgggtacg caggtgggtg agttcttcgg gattgccccc
     3361 aacggcaagc ccgtcaccgc caagggggtg gagacgttcc gcttcgccca tgggtcggtg
     3421 gtcgagatgt ggagtctctt cacgccactg gtggtggtca agcggccgca ggacgaaccg
     3481 gtcgcgctcg atgaggaacc tgagcccgac cgtgtcggac cgctgaagcg gatggcggag
     3541 ttcttgcgca ggaggcccac acgatgatca cggttggttc cgccaccgag gcacggctcg
     3601 gggcgctcga gcacgaagtg gagggcatga ttcgcccgcc ggaccacttc ttcgatccgg
     3661 aggacacgga ggacgtttcc gatctccggc tggtgatgca gcgtctggta cgctggatga
     3721 tcgaaggcta ccagcgggcc aatgcgaccg acctggcgtc ggcggttgct ttccaggcgc
     3781 tcgtggcggt cgtgccgatg ttcctgctgc tcatctcggt cgcgggcctc tttttgcagg
     3841 atggtcgcgt gctgcagcag gcgatcctga cgattgcctg ggtgttgcca tccagcgcct
     3901 cgaatgatgc cttcacggcg gcgttcgagg cacggcgcaa cagtgggctc ttcggcgcgt
     3961 tgagcctgct tggcttcgcg tgggtgggca ccggcttcgt gagcgccatg gcgcgcagca
     4021 tgaaccgcat ctacggcgtg ccgaatgctg gctatctcaa ggagaagcag cgcggattca
     4081 tcgtgatcct gctcttcgcg ctcttcttcc tgatctcggt ggcggcgtcg gtcgtgccga
     4141 ccttcttcgt cgccaacgcc ggcgatctcc cggtggtctt tcggcgctgg gtgctggcca
     4201 gcggacaggg gcagctgctc tcctacgcgc tggcggtggt cgcggcatat ggactcttcg
     4261 tgacgatctt ccggctggtg ccgaacgcca agcagcgcat gcccgacgtc tggcccggcg
     4321 ccgtgatctc gtcgctgctc ttcctgctcg tgacgcaggc cttcccgatc tacctgcgga
     4381 tggtcggcgg cgcgaaccgg tacggcctgg tgctcggtct catatcgctg atggtcatcg
     4441 cattttacgc gctcgcgcat gtgatcctgt tcggtaccta cgtcaatgcc acctggcagc
     4501 gtcaccgcga tgccatgcgg cgacaaaagc ggcgcgaccc gcggagggac accactcccc
     4561 gtccgcctgg ccccgcggcg gcacaggacc cacaggcggt gcgagccgat acgatggagc
     4621 acgtgatccg ctagagcccc gcacacggcg aacggagcct attgccggaa ggtcgtttcg
     4681 acacggaacg gcctgcaggg accggacttg tgccgatccg gcgggcagac acaagatctg
     4741 gccctacggt ttcaatcagc acgacttcta tcagcgctgc gtcggccgtc ctgcaactcg
     4801 ttttcttgtg ggacggtggt catgatcctg ccgggctacc ggcccggtgg gcgcatgttc
     4861 tcgatctgca gtggggtgat gccctcgggg tgcgggaagc cgaagatctg gctgtggaac
     4921 gagagttcgg cctccagtgc ccggtggatt gtctccgccc ggcggaagcc gtgcccctcg
     4981 ccctcgaaca tgagcaacgc caccggcatc ccgttgtcgc gcaccgcctg cgccatcgtc
     5041 tccgcctggt cgggcggcac caccttgtcc tccagcccct gcaggagcag catcgcggag
     5101 ctgagccgct cgacgtggtg gatcgggctg cgcgcctcgt agcgtgcctt ctctgccgga
     5161 taggggccaa ccagcgagtc gaggtagtgc gactcgaact tgtgcgtgtc ccgcgcgagc
     5221 gtctcgaggt cgccgatgcc ataggagctc acgccggacc ggaaggcatc gtggaaggtg
     5281 agcgcggcga gggtggtgta gcctccggcg ctgccgcctt tgatggccat gcggttgccg
     5341 tcgacacgcc cctgctcggc gggccagtgg gccccggcga tgcagtcctc gacgtcgacg
     5401 atgccccagt tgccgatcag ccgctcccga taggcccgcc cgtagccggt gctgccgccg
     5461 tagttgacat cgaggtaggc gaagccgcgg ctcgtccaga actggatgcc aaggtcgaga
     5521 tcggccgtgg tcatgctggt cgggccgccg tgactcttga cgatcagcgg cggccgctcg
     5581 ccagccggcc cggccgcttc cgccgaggcc ggcgggtaga agatgccgtg agcagtcgcg
     5641 ccaccggtcg tgggccagct gatgggctcc ggcacggaga tcgtgccttc gggcaactcg
     5701 atctcggagg agtatcgcag ggtgacgtcg ttgccggatt cgatatccag cgcgatgacc
     5761 tgctttggtg cctcgggcga tgaggcgatc atcagcgcct ggttagtttc cggttgcacg
     5821 tggatgtcgc cgatcacggt gaagggcgtt gcgtacgatc gcagctcgcc gccgtcggtt
     5881 ggcaggcgtc cgatcgacca ggtcccgtct tgtgtccaca ggcagacgat gctgccgtcc
     5941 ggcgcgaagt cccacgtcga catgccgaag acccactgcg gcacgccgaa ctcggcctcc
     6001 atgagacaga gcgcggtggc gtcgtcggcg ctgaggtcct gccggtagag gttccaccag
     6061 ccggtgcggt ccgagacgaa gacgaggctg ccgttgtcgg cccagcgtgg ctggaagatc
     6121 gactcctgcg cgtcaccagc gatgtggcgc agctttctga gcgtggtgcc gcctcccagt
     6181 tcggcggtcc acagctcgca gccgtcccac ggcatcagcg ggtggttcca tgccagccag
     6241 gcgaggtgct gcccatcggc agtgagggca ggggaggcga agaagtctga gccggagacg
     6301 aggacgcggc cgccgtcctc gttggggccg tcaacgtcga gcagcaccag cgtgttgacc
     6361 ggctcggcat ctttcatcga gtggtcctcg cgcaccgcga tgatgcggcc gcgctcggga
     6421 tcaagcagca ggtcggcgta gcgccaatcg gggttctccg gcgtgatcga tgcgggttcc
     6481 gccccatccg tgagacggta gaggcggttg tccgcgtagt tggagactat cacgacgcca
     6541 tcacggacgg cccacgcgcc tcccccgtac tcgtgcacgg ccgagcgaac attgatctcc
     6601 agcggggtga tcggctcggg cttgccatcg ccccgctggc gcacgaggcg gttgacgccg
     6661 gcttcggaag ggcgcgcctc cagccagtag cgtgtggcgc catcggcggc gggcggtccg
     6721 agtcggatga tctgggcggt gagcatctcc gggatgatcg gcgatggcca cgcgccgtag
     6781 gggatgatcc gtttcgctga attcgcgctc atgggattgc ctttccgatc gggctcatgg
     6841 ggaacgtcgg cgcacggaat gatgcgcgtc gtgacatatc gtgcagcgcc gtgtgatacg
     6901 ttcccggcat acttgtgaga gcctagcacg gcgggtcatg cccccgtccg tgcaccattt
     6961 gacccgaaag gttcgatttc tatggcgttg gtcatcgacc atgtctccaa gcggttcggc
     7021 gggttcctgg caatcgacga cgtctcgttc accgttcagg aaggccggat gttcggattc
     7081 ctcgggacga acggcgccgg caagaccacg gcgatgcgca tggtgctcga tatcctgcgg
     7141 cccgacagtg gcaccatcac atggaacggg atgtccaacc ggcagatcgc gcggcaggag
     7201 ttcggctacc tgccggagga gcgcgggctc taccccaaga tgacggtgca ggagcaactg
     7261 ctctttctcg cgcaactcta cggtgcagag aagcgtgcgg cgcaggcggc gctcgacgag
     7321 tggctggcgc ggctggatat cgaggagaac cggcacaaga ccatcgagca gctctccaag
     7381 gggaaccagc agaaaatcca gttcctcgcg gcagtgctgc acgatccgga gatcctgatc
     7441 ctcgacgagc cgttcagcgg actcgacccc gtcaacatcg cccagatgga ggcggcgttc
     7501 aacgagatga aggcgcgggg caagacgatc atcttctcca cgcatcagct ctcgcaggcg
     7561 cagcggctct gtgaggatgt cgcgatcatt catcgcggca ggctgctcgt ggcgggtgat
     7621 gtcgaggcag tgcgcgagag catcgatgtc aagatcctgc gggtcggggt tgccggcgac
     7681 gagcgcctgg agtggctcga tccctatccg gaggtcgaga ttcggcagca gctccgcgac
     7741 catatcgaga tgcagatccc cgatgaggcg gtggcccagc gcatcctgcg cgatgtgctc
     7801 gcgcgcggcg agacggtgga gctcttcgag gtgaccgtgc cgtcgctcaa cgacgtcttc
     7861 ctcacgcgcg tgcggcaatc cggggcgacc gcggaggata tcgcgcggat tgaggcatcc
     7921 aggagcgagc acggggaggc agtagcgtga gtgtccggcc ctccagaatc cgactcgttg
     7981 cacgacgaga gtggctccag aaaatccgcc ggcgcagctt catcatcgcg acgatcgtcc
     8041 agatcgtgat cgtgctcggg ctcatcctcg cgccggtggc gctctcggcg atcttcggcg
     8101 gagacgaggg cacgagtacc gaggaggtgc tgatcgtcaa ccgcaccgac cagcccgaga
     8161 tcgttgccga tacggtcgca ttcatcgcgc atacgggact cggtgaccag atcgaactac
     8221 gggctgcgcc gggcgatacc gatgccgtgc gggcgatcga ggccggcgac gccgatgccg
     8281 tgctcgagat cgagcggggc gccgacgggg cgcttgcctt tacctatacc gacgaggacg
     8341 gcgccaccga ccagacggcg gtggtgatac aggcgctggc cggggagctg gcgctgcggg
     8401 atcaactggt gcgaatcggc gtcgatcccg agcaggcccg agaggtgcgg tccctgccca
     8461 atatggtgat gcaggccgtc ggtgaggccg aggcggacaa cgccgagacc gagcagggct
     8521 tccgctacgg cgtcgcctac gtggccgcgc tggtgatgta catggtggtc ctgatctatg
     8581 gcatgtgggt cgcgcagggc ggcgtggagg agaagagctc ccgcatcatg gagatcatga
     8641 tcaacgcggc gtcgccgacc gagctgatgt acggcaagat catcggcatc gggctcgccg
     8701 ggctgacgca gatcctccca acgctcgcga tcgccggcat cgggctctac tcacaggagg
     8761 cgctggcgga cgcgctcgac gcgaccggta tccacctctc cgggatcgac ttcggcgcca
     8821 tctcggtgca gctggtcggc ttcttcctga tctacttcct gctcggattt ctgttgtacg
     8881 ctgcgctcta cgccggcgtg ggctcgttgg tgagccgcca ggaagacgtg cagacggtga
     8941 tggcgccgat gaccttcacg gtggtgctgg gcttcttcgg ggcgcttttc acgctgggag
     9001 caccggacag catcgtcgcg caggtggtct cgatctttcc atttacgtcc ccgctggcga
     9061 tggtgccccg catcctgctc ggggatccgg agttgtggga gatcctgctc agtatcgcgc
     9121 tgctggcggt cagcggctac ctggccgtca ggctggcggc ccgcatctat cgcatgggcg
     9181 tgctgatgta cggccagaag ccgagcctga aggtgatctt ccggccgggt atcatcacgg
     9241 ccgctcgttg accggggctg ccatatgctg gcgagcctgc tgggcggagg ccagcaggct
     9301 ctgggacgcc cccagaaccc gatgggccgt gcacaaacat ggagacccgg ggtcgcccgt
     9361 gcgagggttg cggtgggggc aggcagggga gtaccggttg gtcgacctgc catcacggtc
     9421 gcggcgctgt tgctgatt
//
LOCUS       DASYWL010000212        10677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_423021, whole genome shotgun sequence.
ACCESSION   DASYWL010000212 DASYWL010000000
VERSION     DASYWL010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10677
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423021"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            492..1925
                     /locus_tag="VGR22_07465"
     CDS             492..1925
                     /locus_tag="VGR22_07465"
                     /inference="COORDINATES: protein motif:HMM:NF011657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HEV2128438.1"
                     /translation="MVTIAFIGAGSTVFARALLRDLFTFPELHDSEIRLMDIDADRLR
                     DSETVARRVADQARADARITATTDRREALSGADYVINMIQVGGYEPATVIDFEIPKKY
                     GLRQTIGDSLGIGGIMRGLRTIPVMLDIGKDMEELCPNALLLNYSNPMAILTWAMNRA
                     TDVRTVGLCHSVFGTAHELATILGLPAGEIDYLCAGINHMAFYLRLEHKGEDIYPRLR
                     QIAAEDGFPSFERVRQSVLRYFGYFVTESSEHFSEYTPWFIKDGRQDLIDQYRIPLDE
                     YPARCVDQINEWAEMRKALLSKDASAMDAYQQNRRSSLSGMWERRLAAVEADSPEEAE
                     QMRAEVLRERSGRESGHSGEYGTLIIHSMETGQPRTIYGNVENAGSISNLPYDCVVEI
                     PCLTNANGFQATQIGEMPPQLTAMMQTNINVQRLGVEAALTQKREYIYHAAMLDPHTG
                     AELSLDEIVSMVDELIEAHGDYLPEYS"
     gene            complement(2085..2291)
                     /locus_tag="VGR22_07470"
     CDS             complement(2085..2291)
                     /locus_tag="VGR22_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128439.1"
                     /translation="MVSRQAGLSEAALLPSGLLSRLATDIEATPVGTPAPGDGTGGVV
                     LLPVESTAILEIGPPNRTPTPDAD"
     gene            complement(2787..2948)
                     /locus_tag="VGR22_07475"
     CDS             complement(2787..2948)
                     /locus_tag="VGR22_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128440.1"
                     /translation="MTVICPRCNARCGDDDLYCSSCGLPLAGIANEREQASDEAGTAR
                     DPVPPAAST"
     gene            3191..4099
                     /gene="murB"
                     /locus_tag="VGR22_07480"
     CDS             3191..4099
                     /gene="murB"
                     /locus_tag="VGR22_07480"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEV2128441.1"
                     /translation="MATTHLSLPNLQYDIPLGPLTTYRIGGPADLFVEVHNRDALAGA
                     VIAARAAGIPWFLLGTGANTLIGDKGFRGLVIQNRADATHIAGTRLMAESGATIARLI
                     TISAEAGLSGLEHFAGIPSSVGGAIWQNLHFLAPGRMRTLFIEDVVESAEILDTSNSR
                     RTVDRDYFEFGYDDSRLHHEEIVVLDVTFALSPKPTAEILHQVRENLRWRVERQPSVE
                     QFPSCGSVFQKIAGVGAGRLIDQVGLKGHRIGGAQISPKHANFLVNVGGATARDVLDL
                     VDLAQREVERQTGYRLEMEIGLVGEF"
     gene            complement(4096..4983)
                     /locus_tag="VGR22_07485"
     CDS             complement(4096..4983)
                     /locus_tag="VGR22_07485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEV2128442.1"
                     /translation="MPALFIVNPVAGRGAGARLLSSLPPTLPADAEVVPTRGPSDASR
                     IAEEAARAGFGPIVAVGGDGTVMETVNGMMCVSSRPSLGILPVGSGNDFARTVGIPRD
                     IGQAIQRIYSHEGDPVDVACCNDRYFLNVGGAGLDTDVARSLERSRSMLLRGQAGYVV
                     HGVKVLSRYRNPLLTVHLDDQVITSRCLLVAVANGRYFAGGMKICPGAEVDDGMLDVC
                     VAGDLSRAEALSQIPMIYFGRHVRHPKIHVHRSRHVRLEQPEGVTVQLDGAVVEYPSA
                     EFSLVPRGLRIAGWRSGPR"
     gene            5385..6044
                     /locus_tag="VGR22_07490"
     CDS             5385..6044
                     /locus_tag="VGR22_07490"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEV2128443.1"
                     /translation="MVFRRESNKVDSFQRQMNALRQQIGDEEQDDEFFDPEASRRTQS
                     ADRGRRPAGDEGYSFGNVPAASSADQNELDMDDVPAIPEMPQVDQQVSVIAAGTTWQG
                     DLEAQGSIHVHGRVNGSLKASEDVWIADGAEVDARIEADRVIIGGDVSGQVTARSRFE
                     ALPECDVQADVDAPTFVVHEGATLNGALSMSRASGGDSNVHESRSRPGSIIQRRGRAS
                     S"
     gene            6088..6654
                     /locus_tag="VGR22_07495"
     CDS             6088..6654
                     /locus_tag="VGR22_07495"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEV2128444.1"
                     /translation="MSLRTHDAPSSGSPGTSESASMIDRHSEFDGTYSTDRDLRIEGR
                     ARGKLSCQGTLHVAQGATVNAEIEAEQVVVAGVLEGTVNCRARLQIMPSGELKGRIST
                     PSLVISEGARYEGQMEMPTQKPRLAARNYPPLAGGSSDRPEAALPEPGTARSSPSTGF
                     IRRLGQQEPREQPEPSTGEDRPGAASES"
     gene            7077..7967
                     /locus_tag="VGR22_07500"
     CDS             7077..7967
                     /locus_tag="VGR22_07500"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEV2128445.1"
                     /translation="MKYPDFFKSLGLQFPGMPRLQLAGENAPLHFSASRGGGGGDYDD
                     APDDDFYDDYEEEPAPRSGGDETRQPTAGRRRTVQSQPDDRYWTDYLRIALPVIGLLL
                     VIAVFWFWAQQLIDDPEDDLVSPTEAPGLAEVVDDSAQVPTDPQQEVVATPPVQQEQQ
                     QQTQAPVVTPTPVVVEQQPAETDQGEVDTAEGADQQVIADPDEGADEQVIAEPEQAEG
                     EPAAGGIGPDSTVVVTEQLNLRSEPTLAADNIVTVLDAGTELTVLSGPTEAEGYIWWE
                     VTDQAGNIGWVAEEFIEPAG"
     gene            8203..9006
                     /gene="rpsB"
                     /locus_tag="VGR22_07505"
     CDS             8203..9006
                     /gene="rpsB"
                     /locus_tag="VGR22_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEV2128446.1"
                     /translation="MAQTQRRNEPVSLKALLEAGVHFGHQKNRWNPKMKRFIFTERNG
                     IHILDLQQSVPMLDNAHEFVSDLTANGKSILFVGTKKQAQDIVRREAERSGQFYVNQR
                     WLGGTLTNWVTIRKRLQTLKRLQQEQAEGQWDLLPKNEASKLQEQLERLERKIGGMRD
                     MTQLPGALFVIDPKREHLAIHEASRLGIPIIAMVDSNCDPDPIDYPIPANDDAIRSIR
                     LLTVGISDAAINGRTELESQQDEEAAMAEAAVEDMDIDDAIREKGDEHE"
     gene            9061..9657
                     /locus_tag="VGR22_07510"
     CDS             9061..9657
                     /locus_tag="VGR22_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEV2128447.1"
                     /translation="MRIVAITTAQIKELREKSGAGIMESKRALEETGGNVDKAIEVLR
                     QQGVAKASKKADRAASQGLVEPYIHGGGRIGALVEVNCETDFVARTPDFQSLAHDIAM
                     QVAATAPRYLSAEDIPTGDYAGLESEFGSREEAVTQVALMDQVFIKNPKVTIREHVKA
                     HIGKLGENILVSRFSRFELGGGSSFAAMTTQPTGTDEG"
     gene            9750..10487
                     /gene="pyrH"
                     /locus_tag="VGR22_07515"
     CDS             9750..10487
                     /gene="pyrH"
                     /locus_tag="VGR22_07515"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEV2128448.1"
                     /translation="MPEPYKRILLKLSGEALMGDQQFGIDPLVAQAMAKELKGVVGTG
                     AQIGIVIGGGNIWRGLSAASKGMDRATGDYMGMIATVLNALALQDALENEDVQTRVMT
                     AIEMRDVAEPYIRRRAIRHMEKERIVILAAGTGNPYFTTDTAAALRALELDADALLMA
                     KNRVDGVYSSDPRTDANAKRYDHITHQEALEKNLRVMDASALALCRDNDLPIVVFDMT
                     ASGSIRRIVNGESLGTLVSSTTNATHR"
BASE COUNT         2097 a         3552 c         3314 g         1714 t
ORIGIN      
        1 cgagcgccgt tacggcgttc cccatgtcgg tcacttccgg cgtgctctcc tgagcggtca
       61 tgccccgcgg cgcaacggtc atcgacgtgg caagcgcgaa cccggcaacg ccgagagaga
      121 aggatcgtcg ggtagtcttc acgcgagtgc tccttttttg gatcaaaccg ttttcgttcg
      181 ggccgcggcg caaatcaacg ggttaaaaat gaacctagag tttagattaa tgtttgtcaa
      241 gccccaaccc gacgggtgct tgcactcgcg cccgttgccc gtgaagatag ggttgctcgc
      301 gttcccagcg gtggccgcaa tcggccgatg aagctagggc cgcatggacg acgccgagac
      361 cgcccaggac ccgcgccgta tgctgggtga gtagcccgcc ggttcctcgt acgcacctta
      421 cgacgacacg cgccggttgc tcgttttgga tgtcctctac ctcggtcacg gttcagcaat
      481 ggagcaacga catggtcacg atcgcgttca tcggtgcggg cagtacggtg tttgcccgtg
      541 ctctcctccg cgacctgttc accttcccgg agctccacga cagcgaaatc cggctgatgg
      601 atattgacgc cgatcgcctc cgagattccg agaccgtcgc ccggcgagtc gccgaccagg
      661 ctcgcgctga cgcaaggatc acggcgacca ccgaccgccg agaggcactt tccggtgccg
      721 actacgtgat caacatgatc caggtcggag gctacgagcc tgccaccgtg atcgacttcg
      781 agatcccgaa aaaatacggg ctgcgccaga ccattggcga ctcgctcggc atcgggggca
      841 ttatgcgcgg cttgcggacc atcccggtca tgctcgacat cggcaaggac atggaagagc
      901 tctgcccgaa cgcgcttctg ctcaactact ccaaccctat ggcaatcctg acgtgggcga
      961 tgaaccgcgc caccgatgtc cgcaccgtgg gtctctgcca cagcgtcttc ggtaccgccc
     1021 acgagctggc cacgatcctc ggccttcctg ccggggagat cgattacctc tgcgccggca
     1081 tcaaccacat ggcgttctat cttcggcttg agcacaaggg tgaggacatc taccctcgac
     1141 tcaggcagat cgccgcagag gacgggttcc cctcgttcga gcgggtgcgc cagagcgttc
     1201 tgcggtactt cggctacttc gtcaccgagt catcggagca tttcagtgag tacacgccgt
     1261 ggttcatcaa ggacggccgg caggatctca tcgatcagta ccgaatccca ctcgacgagt
     1321 acccggcgag atgtgtcgac cagatcaacg aatgggccga gatgcgcaag gcattgctct
     1381 cgaaggacgc gtcggccatg gacgcctacc agcagaaccg ccgatcctcc ctgagcggca
     1441 tgtgggagcg ccggctcgcg gctgtcgagg cggattcgcc cgaggaggcc gagcagatgc
     1501 gcgccgaggt gctgcgcgag cgcagcgggc gtgagtcggg acactccggc gagtacggca
     1561 ccctcatcat ccacagcatg gagaccggtc agccacgcac gatctacggc aacgtcgaga
     1621 acgctggctc gatcagcaac ctgccgtacg actgtgtcgt ggagattccc tgcctgacca
     1681 atgcaaacgg atttcaggca acacagatcg gcgagatgcc gccgcagctg acggcgatga
     1741 tgcagaccaa catcaatgtc cagcggctgg gcgtcgaggc ggcgctgaca cagaagcggg
     1801 aatacatcta ccacgcggcc atgctcgacc cgcacaccgg cgcagaactc tcgctcgacg
     1861 agatcgtctc gatggtcgat gagctgatcg aggcgcacgg cgactacctg ccggagtaca
     1921 gctagccgtc atgcggccta gagggcgaga gcggtcaggg ccaggcagtc cggatttcac
     1981 cggcgagtcg atccacccgc aacgccggca gggcaccggt tctcgctgcg cctgaccgtc
     2041 gccggcctcc gggagcgccg gttctcagca gtgctcagaa cacctcaatc tgcatcgggt
     2101 gtcggtgtac ggtttggcgg cccaatctcc aggattgcag tgctctcgac gggcaggaga
     2161 accacaccgc cggttccatc gcccggcgcc ggtgtgccaa ccggtgtcgc ctcgatatcg
     2221 gtcgccagcc tgctcagcag tcccgacgga agcagcgcgg cctccgacag acccgcctgt
     2281 cgactcacca ccgttgcctc gacagtctgg gtcggctccg gaatcacagt cgcttgcgga
     2341 gccaaggtcg cagcggtttc aggggtcggg gtagctgatg tcgccctcgg tgcaacggat
     2401 acgggcgagg ccggtagggg cgacgccacc accgcctcga ctcccgaggc tgcaggcatt
     2461 tcggcggcga ccgcggggga cgtcaccacg ctcaggctgg actcggtcgg tgtcggcgga
     2521 tcctgaacga ccgcgagctg cccttcggac tcgtcctcgc caccgtcttc ccagaagacc
     2581 agggcagccg cgacacccgc cgccagcagt gcgacaagcg cgaccagcag gagtcatgtc
     2641 cgtccatcac gacgccgact cgatccggag gtatcgaact gcagcggaac gaagtcgaga
     2701 tcgtcgtcga catccgcctc tgaaaggcgc gccgacgctg gctcggacgc cgccgggtcc
     2761 tccccggtct ggcgcttcgg cgttggctac gtggaggctg ccggcggcac cggatcgcgc
     2821 gcggtgccgg cttcgtcgct ggcttgctcg cgctcgtttg cgatcccggc gagcggcaac
     2881 ccacacgaag agcagtagag atcgtcgtcg ccacagcgtg cattgcagcg cgggcagatc
     2941 acggtcatga acggccttgc ttttcaacct gtcccggatt gtcgggggca tgtgacgaga
     3001 gtggacactc cccctgcaac cctgcccaag tgcatcctgt gggcgctcgc aatcgcaggt
     3061 cgtacactga gatggacatc cggccgagtc aagccggtgc tgtcgtggct ccctcccgac
     3121 accgagtata ccgctgccgt gggctcggca cgctgccagg cccggacacg tatcgtggac
     3181 acctgtactg atggcaacca cccacctcag ccttccgaac ctccagtacg acatccccct
     3241 cggccccttg accacctaca ggatcggcgg tccggctgac ctcttcgtcg aggttcacaa
     3301 cagggatgcg ttggccgggg cagtgatcgc ggcccgggcg gccggcattc cgtggttcct
     3361 gctcggcacc ggagcaaata ccctgatcgg cgacaagggg tttcgaggcc tggtgatcca
     3421 aaatcgggcg gatgcgacgc acatcgcggg cacccgactc atggccgaaa gtggcgcgac
     3481 catcgccaga ctgatcacca tcagtgccga ggcagggctc tccggtctcg agcacttcgc
     3541 cggcatcccc agcagcgtcg gcggtgcgat ctggcagaac ctgcacttcc tcgccccggg
     3601 tcgtatgcgc accctcttca tcgaggatgt ggttgagtca gcggaaatcc tcgataccag
     3661 caacagccgc cgaaccgtcg accgcgacta cttcgagttc gggtatgacg acagccgcct
     3721 ccaccatgag gaaatcgttg tcctcgacgt cacattcgcc ctctcgccaa agccgacagc
     3781 agagatcctg caccaggtga gggagaatct ccggtggcgt gttgagcggc agccatcagt
     3841 cgagcaattc ccctcctgtg gatcggtctt ccagaagatc gcgggggtcg gcgccggtcg
     3901 gctgatcgat caggtcggtc tcaagggcca ccgcatcggc ggcgcccaga tttcccccaa
     3961 acacgccaac ttcctcgtca atgtcggagg cgcgacggca cgcgacgttc tcgacctcgt
     4021 cgatctcgcc caacgtgagg tcgagcgaca gaccggctac cgactcgaga tggagatcgg
     4081 actggtcggc gagttctaac ggggtccgga acgccagccg gcaatccgca agcctcgcgg
     4141 gaccagcgag aattctgcag acgggtactc cacgacggcg ccatctagct gcacggtcac
     4201 cccctccggc tgttccaacc ggacgtggcg ggagcgatgc acgtggatct tgggatgcct
     4261 gacatgccgg ccaaagtaga tcatcggaat ctggctgagg gcctcggcac gggagagatc
     4321 gccggcaaca cagacatcga gcatcccatc gtcgacctcg gcgcccgggc agatcttcat
     4381 tccaccggca aagtaccggc cgttggcgac agcgacgagc aggcagcgag aggtgatcac
     4441 ctggtcatcg aggtgaacgg tcagcaaggg atttcgatac cggctcagga ccttcacgcc
     4501 gtggaccacg taacccgcct gcccgcgcag cagcatgctc cgcgatcgct ccagcgaccg
     4561 agcgacatcc gtgtccagcc cagcaccgcc gacgttgagg aagtaccgat cgttgcagca
     4621 tgcgacatcg accgggtcgc cctcgtgcga atagatccgc tgtatcgcct gaccgatatc
     4681 acgaggaatg ccgaccgtcc gtgcgaagtc gtttccactg ccgaccggca agataccgag
     4741 ggacgggcgt gaagacacgc acatcatccc gttgacggtc tccatcaccg tgccatcccc
     4801 gccgaccgca acgatcggac caaagccggc acgagccgcc tcctcggcga ttcgactggc
     4861 gtcgctcggc cctcgggtgg gcacgacctc cgcgtcggct ggcaaggtcg gtggcaggga
     4921 gctcagcaga cgcgcgccgg cgccccgacc ggccacggga ttgacgatga agagcgctgg
     4981 catcgtccac tctgtcctcc acgaacgctc gaatatcgct gcgtgatcgc accagcctcc
     5041 agcatacccg ctggcaggcg catgcgcgtg tcccgcacgg gggtaccgca cagggcaaca
     5101 tcgcggactc attcatttgg cacgacttgg cacggtggag gtcgtgtgac cctgcccgac
     5161 cggggacatg gtgtcaaggc tgtacgaaca ccgcaggagt gtgatattgt cgcgtgaaac
     5221 gacgcccgtc gtggtaggat gaacgtacgt tctccctgct ggccgacagt gcagccgcgg
     5281 ccagcaacgg cagccacgta cgccctcccc caccaccagt tccgggctgc agggatggtg
     5341 gccttcctga caggtggttg ctgtgtacca gaggagctcc tggcatggtt tttcgacgag
     5401 agagcaacaa ggtagattcc ttccagcgcc agatgaacgc gttgcgccag cagatcggcg
     5461 acgaggagca ggacgacgag ttcttcgatc ccgaggccag ccggcggacc caatccgctg
     5521 atcgcggccg gcggcccgcg ggtgacgagg ggtacagctt cggcaatgtt cccgctgcgt
     5581 ccagcgccga ccagaacgag ttagacatgg acgacgtccc ggccatcccc gagatgcccc
     5641 aggtcgacca gcaggtcagc gtgatcgctg ctggcacgac atggcagggc gacctcgagg
     5701 cccagggctc tatccatgtg cacggccgcg tcaacggttc cctcaaagcc agcgaagacg
     5761 tctggattgc cgatggagcc gaggtcgacg cccgcatcga agccgatcgc gtgatcatcg
     5821 gtggcgacgt gagcgggcag gtcaccgcgc gctcccgatt cgaagcgctg cccgaatgtg
     5881 acgtgcaggc cgacgtcgat gcccccacct tcgtggtcca cgaaggggcg acgctcaacg
     5941 gggcgctctc gatgagcaga gcgtctggag gcgacagcaa cgtccacgag tcacgctcgc
     6001 gccccggctc gatcatccag cgccgcggac gcgcctcgtc ctgatcgcca gccgggctcc
     6061 gcccatcacc tcagcaggag aaatgccatg tcccttcgaa cacacgatgc cccctcctcc
     6121 ggcagccctg gcaccagcga atccgccagc atgatcgacc gccactccga attcgacggc
     6181 acctactcca ccgatcgtga cctccgcatc gaaggacgag ccaggggcaa gctcagttgc
     6241 cagggcacgc tccacgtggc gcagggcgcc accgtcaatg ccgagatcga agcggagcag
     6301 gtcgtggtgg ccggcgtcct cgaaggcacc gtcaactgtc gcgcgaggct gcagatcatg
     6361 ccaagcggtg agcttaaggg ccggatctcg accccgtcgc tggtgatcag cgagggtgcc
     6421 cgttacgagg gtcagatgga gatgccgacc cagaagccac gtctcgcggc acggaactac
     6481 ccgccgcttg ccggcggatc ctcggaccgc cctgaagccg cgctcccgga acctggcacc
     6541 gcccggtcat cgccgtcgac gggattcatc cgacggctag gccagcagga accccgcgaa
     6601 cagccggagc catcgacggg cgaggaccgc cccggggctg ccagcgaatc ctgaggacac
     6661 caccgtcacg gaaccacatc cacgcccggg tcgttacact tgcaggagcg acccgggcgt
     6721 cggcgcctcc accggccggg cctgcggaga tcgatccagc atcagtgcgt gatggacgag
     6781 cgggtccgtc atgaccatct tcgattccat gactcgttgc gttatgcttc ctgctgagag
     6841 gaattgacgg tgaccgagca tcgccggtgt ggcacctgct ggttttacga gccgtctccc
     6901 atgtggcggc gtggctggtg ccgaaacaca acgctgaatc ggctgggcta cagccaccca
     6961 gttgaagaag acgaacatga ctgtcgacgt gggcgcagtg actattggca gcctgcggac
     7021 ccggaacggc gcgaggagta caaccgtcac cgcatgaagt atcgaggcga cagactgtga
     7081 aatatcctga cttcttcaaa tcgctggggc ttcagtttcc cggcatgcct cgcctccagc
     7141 tggccgggga aaacgctccg ctgcacttct ctgccagccg cggcggtggt ggcggcgact
     7201 atgacgacgc ccccgacgat gacttctacg acgactacga ggaagagccg gcgccccgga
     7261 gcggtggcga cgagacacgt caacctaccg ccggccgccg ccgtaccgtg caatcgcaac
     7321 cggatgatcg ctactggacc gattatctgc gcattgcgct gccggtgatc ggactcctgc
     7381 tcgtcatcgc agtcttctgg ttctgggcgc agcagctcat cgacgacccc gaggacgact
     7441 tggtgtcacc taccgaggcg ccgggccttg ccgaggtcgt cgacgactcc gctcaggtcc
     7501 caaccgaccc ccagcaggaa gtagtggcca ccccgcctgt gcagcaggag cagcagcagc
     7561 agacccaagc ccctgtggtc accccaacgc cagtagtcgt ggagcagcaa ccggcagaaa
     7621 cggaccaagg cgaggtagac accgccgagg gggctgacca acaggtgatc gccgaccccg
     7681 acgagggtgc cgacgagcag gtcatcgctg aaccggaaca ggccgagggg gagccggcag
     7741 cgggcggtat cgggccggat tcgacggtcg tcgtgacaga acagttgaac cttcgctcag
     7801 agccaacatt agctgcggac aacattgtga ccgtccttga cgcaggcacc gagttgacgg
     7861 tgcttagcgg gccgaccgaa gccgagggat acatctggtg ggaagtcact gaccaggccg
     7921 gcaacatcgg ttgggtagcc gaggagttca tcgagcccgc cgggtagccc gacaccgggg
     7981 cacagccgat acacgcagca accaacgtcc cggtgtcgtc tcgacggctg ccaggacttc
     8041 ccgtgcccgg tatgcccgga aggcatggta tactccccgc cgggtttggc gctctgccag
     8101 accctttatt gagcacgcgt ggtgactggt gacggtgtgc ccagcatggg ttcggcaccg
     8161 ggatgatccc cgcggaagat acaacacacg agagggttat tcatggcaca gacgcaacga
     8221 cgaaacgagc ccgtcagcct caaggcgctg ctcgaggcag gtgtccactt cggacaccag
     8281 aagaatcgct ggaatccgaa gatgaagcgg ttcatcttca ccgagcgcaa cggcatccac
     8341 atcctggacc tccagcagtc ggtgccaatg ctggacaacg cccacgagtt cgtcagcgac
     8401 ctcaccgcca acggcaagag catcctgttc gtcggcacca agaagcaggc gcaggacatc
     8461 gtccgacgcg aagctgagcg cagcgggcag ttctacgtga accagcgctg gctcggcggc
     8521 accctgacca actgggtcac cattcgcaag cgccttcaga ccctcaagcg actccagcag
     8581 gagcaggccg aaggacagtg ggatctgctg ccaaagaacg aggcttccaa gctgcaggag
     8641 cagctcgagc gcctcgaacg caagatcggc ggcatgcgcg acatgacgca actccccggc
     8701 gcgctcttcg tgatcgatcc aaagcgcgag catctggcca tccacgaggc gtcccggctc
     8761 ggcatcccca tcatcgcgat ggtcgacagc aactgtgacc cggatccgat cgactacccg
     8821 atcccggcga acgacgacgc catccgctcg attcgcctcc tcacagtcgg tatctccgat
     8881 gccgccatca acggtcgcac cgagttggag agccagcagg acgaagaggc cgccatggct
     8941 gaagccgccg tggaagacat ggacatcgat gacgccatcc gcgagaaggg tgacgagcac
     9001 gagtagtccg cggctgcagt tgtcaggaca catcacaatc aagcaaacac agagaggtgg
     9061 atgcgcatcg tggctattac aaccgcacag atcaaggagc tccgcgagaa gtcgggcgcg
     9121 ggcatcatgg agagcaagcg ggcacttgag gagaccggcg gcaacgtcga caaggccatc
     9181 gaggtcctcc gccagcaggg cgtcgccaag gcgagcaaga aggccgaccg ggccgcttcc
     9241 caaggcctcg ttgagcccta catccacggc ggtggtcgca tcggcgcgct tgtggaagtg
     9301 aactgcgaga cggactttgt cgctcgcacg ccagacttcc agtcgcttgc ccacgacatc
     9361 gccatgcagg tggccgccac ggccccacgg tatctgtccg ccgaggatat ccccacgggc
     9421 gactacgccg gcctcgagtc ggagttcggc agccgcgagg aggccgtcac acaggtcgcg
     9481 ctgatggatc aggtgttcat caagaacccc aaggtgacca tccgcgagca tgtcaaggcg
     9541 catatcggca agctgggcga aaacattctc gtcagccgct tcagccggtt cgagcttggc
     9601 gggggcagca gtttcgctgc gatgacgaca cagccgaccg gcaccgacga agggtaatcg
     9661 ctgcccccga cgccgtcttt cctcggaagg gcggcgtcgt gccgtatgcg gcaacccggc
     9721 gccacccagc atctccacgg agcctcacca tgccagagcc ctacaaacgg atcctcctca
     9781 aactcagcgg cgaagccctg atgggcgatc agcagttcgg catcgacccg ctcgtggccc
     9841 aggcgatggc gaaggagctc aagggggttg tgggcaccgg cgcgcagatc gggatcgtca
     9901 tcggcggcgg caatatctgg cgtgggctct ccgccgccag caaggggatg gaccgggcca
     9961 cgggcgacta catgggcatg atcgcgactg tgctgaacgc tctcgcgctg caggatgccc
    10021 tcgaaaacga ggacgttcag acgcgcgtga tgacagcgat cgaaatgcga gacgtcgccg
    10081 agccatacat ccgccgccgc gcgatccgcc acatggagaa agagcgcatc gtcattctcg
    10141 cagccggcac cggcaacccc tacttcacca ctgacacggc ggccgctctc cgcgcactcg
    10201 agctggatgc cgatgccctg ctgatggcca agaaccgggt tgatggcgtc tactcctctg
    10261 accctcgcac ggacgccaac gccaaacgct acgaccacat cacgcatcag gaagccctgg
    10321 agaagaatct gcgggtcatg gacgcctccg cgcttgcgct ctgccgggac aacgatcttc
    10381 ccatcgtcgt tttcgacatg actgccagcg gcagcatccg ccgcatcgtc aacggcgaat
    10441 cgctggggac tctggtatcc tcaaccacga acgccacgca tcgttgagcc atctcctctc
    10501 tgccagcttc aaccctgaat caggaaagga cgggacctgc atgacagccg agatcctgaa
    10561 ggacgcggag tcgcggatga ccaaggcgat ggacgccctg caccacaatc tcgccaccat
    10621 ccgtaccggt cgtgccaccc ctgcactgct cgacagaatc catgtcgact actacgg
//
LOCUS       DASYWL010000213         8687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_160304, whole genome shotgun sequence.
ACCESSION   DASYWL010000213 DASYWL010000000
VERSION     DASYWL010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8687
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160304"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..1432
                     /locus_tag="VGR22_07520"
     CDS             131..1432
                     /locus_tag="VGR22_07520"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2128449.1"
                     /translation="MSTAAPHIQETQQRVVRVLAAAQVLGGIGVASGAAVGALLAAEL
                     ATDSLSGLAAASSVIGAALIAIPVSRLMNVSGRRSGLLLAYAIGIIGAMLVVFGAVID
                     QFLVALVGLIAMGGGTTAGLQSRYAATDLATPDRRGRSLSTVVWATTIGSVLGPNLAD
                     PMGDVAEAIGVPRLAGPYLMSLGVFSISAAMIGLLLRPDPLIVARDELERTTGVKGGP
                     ALQTSIAAVLRSISGITPAVLGLLAIVVGHASMVGVMAMTPVHLRHDGASLRIIGFVI
                     SGHITGMYIASPLVGLAADRIGRRPVIITGGAFLLSAFLIAGTATGHESTQLAVGLFL
                     LGLGWSCTLIAGSTLLTESVPVDDRPNVQGTSDLLMGIGGASAALLSGVVVGFGSYGL
                     LNLITTLLVVPMIVLAVRDALRHRHPVTQPPGAPTPTAQRR"
     gene            1579..2499
                     /locus_tag="VGR22_07525"
     CDS             1579..2499
                     /locus_tag="VGR22_07525"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEV2128450.1"
                     /translation="MATDQQTAAQQQFTEAMEGFEARMGGTLGVMARDLNTGLELAWN
                     HERPFPTASTMKVPLLYGLYRLAETGEIGLTTRVPLRKDDRVPGSGVLQHLDAGLEPT
                     VRDLAELMIIVSDNYATDLLYATVGRERLAGTLAELGLDQTYLPHTVWQMLSHVGGVD
                     AEDPELTYDELRERLKGSREPDAPDPYQSDEYDRSTPADIVRLMTRIEEGYGVSRASR
                     GAMIEILKHQTINDRIPVRFPEELDIETAHKTGSLRGVRNDVGIVYAPGVTYTIAIMT
                     RDLPDPVVAVPEIAAASRWVFDLLTDRAAS"
     gene            complement(2540..4015)
                     /locus_tag="VGR22_07530"
     CDS             complement(2540..4015)
                     /locus_tag="VGR22_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128451.1"
                     /translation="MRDKGGDGNLLANARALLIGVLVSIGVILTVAAVVAVLVWASMA
                     VGVLGMGRQWTAAVIGVVAVVGLVGSFAVGSWTGIRRTSRAGSLVIGGLVPLLVIVVT
                     GLAVWPLIASMVDVRSAAIELGWIAAPDAGVVIDQDLPTQAELQQGWEEPGGSGPLSD
                     PVWHLVAAIAFLGALLVAVILAGMLGAALNRRRRDSAGPRMMSRDRASPSLVILGAGI
                     ALLTVVLWSSLWTTGAAFVDIDQSAGPDVGVNLREVANHPEAMWGQSVTVSAAIDRLV
                     GRHAAIIGSDTPLIGSKVLIVSEPELQEQVLAASSQGAELSEGDVVQVTGTVQRYDRE
                     SLAAELGVRLDEDADSGYDGSAVLLVSSINLDVPVAVEGGDKEFVGGSDGYDRGVTIN
                     DIVAAPVDHVGMTVTVSAEVEGDTLASHVFTIGDGFFLVVSAKPHPEVFREATAYVTG
                     EVGIFDLARVEEQIGVDLDDEALSEYEGEPFILAEAIQVVA"
     gene            4708..5778
                     /locus_tag="VGR22_07535"
     CDS             4708..5778
                     /locus_tag="VGR22_07535"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV2128452.1"
                     /translation="MIWSAMHQQQQAKLDKSDTQADRSHADIAPPVRKNSIGDPSAQT
                     SPLIALVRPRAPQSARSRLAVIGGVIALAVAIHWLTGGRWTPVLIGVAGAVSLLALAP
                     PLAWLAKPVGAYAGLWVVFNALRAGADNTDWSDQVLGLVPRLEAWLAAGHLPSAWLQD
                     RFHEANSFGWHDYGLTIVYLSFFIVPYATAGLLLWCNRRLFWQYTVATAALFAVSTVS
                     FYVIPTSPPWLISDVAVQGDFEPVRRITESVLTSVDLPVQVFSEGSRRGARTSEVRVE
                     PNPIAAMPSIHFAATALLIGPARRAGSGLLAAIVVYVFLMGLALVYLGEHYILDLLAG
                     GVLAAIGWFLAGCWLAAANVAP"
     gene            5806..7056
                     /locus_tag="VGR22_07540"
     CDS             5806..7056
                     /locus_tag="VGR22_07540"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV2128453.1"
                     /translation="MTTHTTATPPSDKGSPAGQRAWLRRVLIGLVILLLTGIAGFGAL
                     VEFADYCESDFLPFEESISAEPYVQNVSSNAATVMWRTEERTDGYVVFGTAGEADRTV
                     ASDSSTVHAVRLANLRPDTEYVYEVRSGESVYDSATFNTAPGPDGEVTAIVVGDTGSG
                     EPPQYEVAEVMMDINADLLLHTGDVVYPHGGTCRYEERYYAPYREMIESVPVYPVLGN
                     HDVRTDNGEPYLTAFELPAESSGTERYYSFDYGPLHVAALDSELYYNDDTIPTERQKA
                     WLREDLAASDRPWKVVIIHRPPYSSSPFHGGDRQILEDLVAIFEEEEVDVVFAGHEHV
                     YERLVPIDGVTYFVTGGGGADLRSAGRSDLTAVSSLRYHALEVKASPERVLINAVGID
                     GEVFDTIELTQPDPSVVTPVRPPG"
     gene            7265..8224
                     /locus_tag="VGR22_07545"
     CDS             7265..8224
                     /locus_tag="VGR22_07545"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEV2128454.1"
                     /translation="MTTKPCRSWSKRTRRDAIAGGIGIGTAVVTGAVPRRGVATQQTP
                     QASPASSGGYARSEPLVAAAWLKAHLTDPGMVVVAFMPVEEAEAEHIPGAEQIDWPVL
                     EVTDTSDASLTSWQESVEGLLGVLGITQDSTVVVYDAGTLFAARLWWILHWLGHEQVH
                     VLDGGLAAWKASGFEVQHGPITPSESTHLPYNGQPNGTVLAQLDEVLQALDDANTTIV
                     DARTPSEYADGHIPGAVNINYPRNAYDEPPKRYRPADELLEMYAEAGVTTDRRVIPYC
                     LTGVRSAVTFFTLHLLGFDELALFTGSWEEWSSDPNTPKATGQ"
     gene            8409..>8687
                     /locus_tag="VGR22_07550"
     CDS             8409..>8687
                     /locus_tag="VGR22_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128455.1"
                     /translation="MGDKQATSADLGVACQVEVPADQQDRDDGRDEQDLNQGRRIDVT
                     VPRTGSPEGVSGHERHPVLHTSDCTGSSIDDRTAGREQIICLPTTTSDQ"
BASE COUNT         1545 a         3046 c         2636 g         1460 t
ORIGIN      
        1 gcttgcgggg gcagcttcca tgcctgtttc tgcaatgccg cggacgtgag acaatcggga
       61 cgtcgggcca gcatggccca tcttccgggc tccgacccaa ccagacgcat cccggagagt
      121 cgatcaccac gtgagtaccg cagctcccca tatccaggag acacagcagc gcgtcgtccg
      181 cgtgctcgcc gccgcgcagg tgctcggtgg aatcggcgtc gcctcgggcg ccgccgtcgg
      241 cgcgctgctc gccgctgaac tcgccaccga ttccctcagc ggcctcgcgg cagcttcctc
      301 cgtcatcggc gcggcgctca tcgccatccc ggtctcccgt ctcatgaacg tcagcggtcg
      361 gcgatctggc ctcctgctcg cttacgcgat cggcatcatc ggcgccatgc tcgtggtctt
      421 cggcgccgtc atcgaccagt ttctcgtcgc gctcgtcggg ctgatcgcga tgggaggcgg
      481 cacgacagcg gggttgcagt cccgctacgc cgcaaccgac ctcgccacac cggaccgacg
      541 cgggcgatca ctctcgaccg tcgtctgggc aaccacaatc ggctccgtgc tcgggccgaa
      601 cctggccgat ccaatgggcg acgttgccga ggccatcggc gtgccccggc tcgctggacc
      661 ctatctgatg agcctcggcg tcttcagcat atccgcggcg atgatcggcc tcctcctccg
      721 gcccgacccc ctcatcgttg cacgcgatga gctcgagcgg accactggcg tgaagggcgg
      781 accggcgctg caaacctcga tcgccgcggt gctgcgcagc atctcgggaa tcacgccggc
      841 agtgctcggg ttgctcgcga ttgtcgttgg ccacgcctcc atggtcggcg tgatggccat
      901 gaccccggtc catttgcgcc acgacggtgc ctcgctgcgc atcatcgggt tcgtgatcag
      961 tgggcatatc accggcatgt acatcgcctc gccgctggtc ggcctcgccg cagatcgcat
     1021 cggccgccgg ccagtcatca tcaccggagg agctttcctg ctcagcgcct tcctgattgc
     1081 gggcaccgcg accggccacg agtcgaccca actcgccgtc ggcctcttcc tgctcggtct
     1141 cgggtggtcg tgcacactga tcgccggatc gacgctgctc acggagtccg ttccagtcga
     1201 tgatcgaccg aacgtgcagg gcacctcgga cctgctaatg ggcatcgggg gcgcgagcgc
     1261 tgcactgctc tccggggtcg tcgtcgggtt cggctcctac gggctgctca acctcatcac
     1321 gaccctgctg gtcgtgccga tgatcgtcct cgcggttcgt gacgccctgc ggcatcgcca
     1381 cccggtaacc caaccgcccg gcgcccccac tccaaccgcc cagcgccgat gatctggccg
     1441 cagcatgggc acactccgcg cgtgccctgc ctgtttcccg agcacagagg gtacgcaatg
     1501 cggatgtgaa cagtggggga ccgcccggcg ctcatcgaga tttggcatga gactgattcg
     1561 acagagacgg aggaccacgt ggcaaccgac cagcagacag cagcccagca gcagttcacc
     1621 gaggcaatgg agggattcga ggccaggatg ggtggcacgc tcggcgtcat ggcccgcgac
     1681 ctcaacaccg gcctcgagct ggcgtggaac cacgagcgcc cgttccccac cgcctccacg
     1741 atgaaggtgc cgctgctcta cgggctctac cgcctcgcgg aaaccggcga gatcggcctc
     1801 accacacggg tgccgctccg aaaagacgac cgcgtaccgg gatccggcgt gctccagcac
     1861 ctcgatgccg gcctggagcc caccgtgcgc gacctcgccg agctcatgat catcgtttcc
     1921 gacaactacg cgaccgattt gctctacgcc accgtcgggc gcgagcgcct ggcggggacg
     1981 cttgcggagc tggggctcga ccagacgtac ctgccccata cagtctggca gatgctctcc
     2041 cacgtcggtg gtgtcgacgc ggaggatccc gagctcacct acgacgagct ccgggaacga
     2101 ctgaaaggca gccgggagcc ggacgcaccg gacccatacc agagcgacga gtacgaccgg
     2161 agcactccgg cagacatagt gcgactgatg acgcgcatcg aagaggggta cggggtgtca
     2221 agggcgagcc gcggggcgat gatcgagatc ttgaaacacc agacgatcaa cgaccgcatc
     2281 ccggtccggt ttcccgagga gcttgacatc gagaccgcgc acaagaccgg cagcctgcgg
     2341 ggcgtccgca acgatgtcgg catcgtctat gcgccggggg tcacctacac cattgcgatc
     2401 atgacccgcg acctcccgga ccccgtcgtc gcagtgccgg agatcgccgc cgcctcccgc
     2461 tgggtgttcg atttactgac ggatcgcgcc gcaagctgag gctgcgggct cgagtcgcgc
     2521 acgaggtgcc gagcgtccgc tatgccacaa cctggatggc ctccgcaagg atgaacggct
     2581 caccttcata ctcgctcaat gcctcgtcgt cgagatcgac cccgatctgc tcctccacgc
     2641 gggcgaggtc gaagatacct acttcaccgg tgacgtacgc cgttgcctcc cggaatactt
     2701 cgggatgggg cttggcgctg acgacgagga agaacccatc accgatagtg aacacatgcg
     2761 aggcgagcgt atccccctcg acctcggcgc tcaccgtcac tgtcatgccg acatggtcca
     2821 ccggcgcggc gacgatatcg ttgatagtca cgccccgatc gtaaccgtcc gacccaccta
     2881 cgaattcctt gtcaccgccc tccacggcga ccggcacatc taggttgatt gacgacacta
     2941 ggagaacggc tgagccgtcg tatccgctgt ccgcgtcttc atcgagcctg acccccagct
     3001 ccgctgcgag cgattcccga tcataccgct gcacggtgcc cgtcacctgc accacatcgc
     3061 cctcgctcaa ctccgcgcct tgtgacgaag cagccagcac ctgctcctgc agctccggct
     3121 cgctgacgat caggaccttg cttccgatga gcggtgtatc gctcccaatt atggcggcgt
     3181 gccggccgac cagtcggtcg atggccgcgc ttacggtgac agactggccc cacatcgcct
     3241 ctgggtgatt ggcgacttcc ctcaggttga cacccacgtc aggaccggct gactggtcaa
     3301 tgtccacgaa tgcagcccct gtcgtccaca ggctcgacca gagcacgaca gtgagaaggg
     3361 caataccggc ccccaggatc acgagcgacg gtgatgcccg atcccgagac atcatcctcg
     3421 gtcccgccga gtccctgcgt cgcctgttca gggctgcgcc cagcatgccg gcaaggatca
     3481 ccgccaccaa cagggcgccg agaaaggcga tcgccgccac cagatgccag acaggatcgc
     3541 tgaggggacc tgagcctcca ggctcttccc acccttgctg cagctccgcc tgagtcggga
     3601 gatcctggtc aatcacgacg ccggcatccg gagcggctat ccagccaagc tcgatagccg
     3661 cgctccggac atccaccatc gaggcaatga gcggccaaac ggccagccct gtcaccacaa
     3721 taacgagcaa cggcacgagt ccaccgatca cgagcgagcc ggcgcgactc gtccttctga
     3781 tgcccgtcca ggagcccacg gcaaacgagc caaccagacc taccacggcg accacgccga
     3841 tcacggctgc ggtccactgc cgccccatcc ccaacacgcc caccgccatg ctggcccaga
     3901 ccaataccgc cacaactgcg gcaaccgtca ggatcacccc gatgctcacc aggactccaa
     3961 tcaggagtgc ccgcgcgttt gccaacaggt tgccatcgcc gcctttgtct ctcatgtggc
     4021 ccggtgtcct ctcgacatcc gtctcgcgat ccctgacgtc ctctcgctca gcaacgtatc
     4081 caataggttc ctcacctagc cccgtcacag atgcggaccg cttcatcctc cgatcagccg
     4141 ccctcacatg aagcgaagtg gcacgctgat tacaggtaca tgcacgataa atgccgacat
     4201 caccttggcc ggtggtgtat ggagaacgga ggtgcgtgga ctgagagtgg gggccagcgg
     4261 accatgtatc gaaggaactg ggtgaaggct agcgcgcagg tggcagcagg gggcccctct
     4321 ccgccccggg ccatcctctt gcagcgtcgc cacacctgcc tgcaacgaga tcggcgctcg
     4381 tgagcgacgg atccaccgaa gcgaatccaa atggtacacc cacgcagccc accgtccctg
     4441 cacagctggc tggcagccga cgccggccac gagcgatggc caccaccttc ctgtcacgcc
     4501 gctgctgcgc cctctcacag gattcgtcct cgtaccccct tgcgtgatgc agtaccacgt
     4561 ccgtcggcaa tcgacgccgc gcacggaatc ggtatcgcgg gcgccatcct ggaagcggca
     4621 cagtgattgc gaagatgcga tccgggtatc accgaggcga cagcccggat gtcaccccgt
     4681 tgcgccggtg gcggcgctgg atgtgccatg atttggagcg caatgcatca gcaacagcag
     4741 gccaagctcg acaagtcaga tactcaggcc gatcgcagtc acgccgacat cgcgccacct
     4801 gtccgcaaga attccatcgg cgatccatcg gcgcaaacct cacccctgat cgccctggtg
     4861 cgaccgcgag caccacagtc ggcccgctcg cgcctagcag tcatcggtgg agtgatcgcg
     4921 ctcgcggtgg ccatccactg gctcaccggt ggccgctgga cgcccgtgct gatcggcgtg
     4981 gcgggagccg tctccctcct cgccctggcc ccgccgctcg catggctcgc caagccggta
     5041 ggcgcctatg ccgggttatg ggtcgtcttc aacgcgctgc gggcgggcgc ggataacaca
     5101 gactggtccg accaagtcct cgggctggtg ccgcgcctcg aggcatggct cgccgcgggc
     5161 catctcccgt cagcatggct ccaagaccga tttcacgaag ccaacagctt cggctggcac
     5221 gactacggcc tgaccatcgt ctacctctcg ttcttcatcg ttccctacgc gaccgccggc
     5281 ctgctcctct ggtgcaatcg acgtctgttc tggcagtaca ccgtcgccac ggcagccctg
     5341 ttcgccgtct cgaccgtgag cttctacgtg atcccgacga gcccaccatg gctcatttcc
     5401 gacgtcgccg tgcagggcga tttcgagcca gtcaggcgca tcaccgagtc ggtgctgacc
     5461 agtgtcgacc tgcccgtgca ggtcttctcc gagggatccc gccggggggc ccgcacgagt
     5521 gaggttcgcg tcgagccaaa cccgatcgcc gccatgccgt cgatccattt cgcggcaacg
     5581 gccctcctga tcggtccggc ccgccgcgca ggctccgggc tgctcgctgc tatcgtcgtc
     5641 tacgtattcc tgatgggcct cgcgctcgtg tatctcggcg agcactatat tctggatctc
     5701 cttgcgggcg gcgtgcttgc tgcgatcgga tggttcctcg ccgggtgctg gctggccgcc
     5761 gccaacgtcg cgccttgatc gacccgcgaa cggaaaccat cagccgtgac cacgcacacc
     5821 accgccacac caccgtcgga caaaggctcg cccgccggcc aacgcgcatg gctgcgccga
     5881 gtgctcatcg ggcttgtgat cctgctcctg accgggatcg cagggttcgg tgctctcgtt
     5941 gagttcgccg actactgcga gtcggacttt ctcccgttcg aagagagcat ctccgccgag
     6001 ccgtacgtac aaaatgtgag ctcgaacgcg gcgaccgtca tgtggcgcac tgaggagcgc
     6061 accgatggct acgtagtgtt cggcaccgcg ggagaggccg atcgcacggt cgcgagcgac
     6121 tcgtccaccg tgcacgccgt tcgcctggcc aatctacggc cggacaccga gtacgtctac
     6181 gaggtgcgat ccggcgagag cgtctatgat tcggccacgt tcaacaccgc gccgggaccc
     6241 gacggcgaag tgaccgcaat cgtggtgggg gatacgggct caggcgagcc cccgcagtat
     6301 gaggtggccg aggtgatgat ggacatcaat gctgacctcc tcctccacac aggtgatgtc
     6361 gtctacccgc atggtggaac atgccgctat gaggagcgct actacgcccc ctatcgcgag
     6421 atgatcgaat ctgtacccgt ctaccccgtg ctgggcaacc acgatgtccg aacggacaat
     6481 ggcgagccgt acctcaccgc gttcgagctg ccggccgaga gctccgggac ggaacggtac
     6541 tactccttcg actacggccc gctgcacgtc gcggcgctgg acagcgaact ttattacaac
     6601 gacgacacga tccccaccga gcggcaaaaa gcctggctac gcgaggacct cgcggcctcg
     6661 gaccgtccat ggaaagtggt gatcatccac cgcccgccct actcctcgtc acccttccat
     6721 ggcggcgatc ggcagatcct cgaagatctg gttgccatct tcgaggagga ggaggtcgac
     6781 gtcgtcttcg ccggtcacga acacgtctac gagcggctcg tcccgatcga tggcgtcacc
     6841 tacttcgtca cgggtggcgg tggtgctgac ctccggtctg caggccggag cgatctgacg
     6901 gcggtcagct cgctccggta ccacgcgctc gaggtcaaag ctagccccga acgggtcctg
     6961 atcaatgcgg tcggcatcga cggcgaggtg ttcgacacca tcgagctgac gcaaccggat
     7021 ccctcggtcg taacgcccgt cagaccaccg ggttgaggtt gccggacggc cgcggccgtc
     7081 ccggtccacg cccgccttca tggaggcgcc tgccatcctc gccgacgtga agccactcgg
     7141 ccaattgctg ccacggcgag caaatagctg ccaacggcac catgctgtca agtggagcac
     7201 ggtcatgata gtgtgagctg acaatcgggc acatccgagt cgacttcacg tgtaggacgc
     7261 cgccatgaca acgaagcctt gccgcagctg gtcgaaacgg acgcgccgag acgcgattgc
     7321 cggtggcatc gggatcggca cagccgtggt gaccggtgca gtgccacgcc gcggggtggc
     7381 aacccaacaa acgccgcaag catctccggc gtcgagcggg ggatacgcgc gatccgagcc
     7441 gctcgtcgcc gccgcgtggc tgaaggccca cctcaccgac ccaggcatgg tggtcgtcgc
     7501 cttcatgcca gtggaggagg ccgaggcgga gcacatccct ggtgcggagc agatcgactg
     7561 gccggtactc gaggtgaccg acacctccga tgcctcgctc acgagctggc aggagtcggt
     7621 tgaggggctg ctcggcgtcc tcggcatcac tcaggacagc acggtcgtgg tctacgacgc
     7681 cggcaccctt ttcgccgcgc ggctctggtg gatcctgcac tggctggggc acgagcaggt
     7741 gcacgttctc gacggcgggc tggctgcctg gaaggcatcc gggttcgagg tgcagcatgg
     7801 cccaatcaca ccgtccgaga gcacccacct gccctataac gggcagccaa acggtaccgt
     7861 tctcgcccag ctcgacgagg tgctgcaggc actcgacgac gcgaacacca ccatcgtcga
     7921 tgcccggacg cccagcgagt atgctgacgg gcacatcccc ggcgcggtca acatcaacta
     7981 tccgaggaat gcttacgacg agccgccgaa gcgctaccga ccggccgacg agctgctgga
     8041 gatgtatgcc gaagccgggg tgactacgga ccgccgggtg attccctact gcttgaccgg
     8101 agttcggtca gcggtgacat tcttcacgct gcacctgctc ggcttcgacg agcttgccct
     8161 gttcaccggc tcatgggagg agtggagctc ggacccgaac acaccgaagg caaccgggca
     8221 gtaaggtgtg actgctcctg gtcgccggac aggtcttctg gaccggcccg gaacccggcc
     8281 gacgtcatga aacaaggcgg ccgagccctt cccccgccga cgaccgccgc gattggcttc
     8341 cagatggggt gcgcgaagat gaagagcatt cccagtgcga tcgtcatacc caggcctccc
     8401 cacaccttgt gggcgataag caggctacgt ccgccgacct gggggtagcc tgtcaggtag
     8461 aggtacccgc ggatcaacag gatcgcgacg acggccgtga cgagcaagat ctcaatcagg
     8521 gccgtcgcat tgatgtcacg gttccgcgaa ccggctcgcc ggagggggtg tctgggcatg
     8581 aacggcatcc tgtgctgcac accagcgatt gcacaggctc cagtatcgac gatcgcactg
     8641 ctggtcgcga gcagatcatc tgtcttccca ccaccacgag cgaccaa
//
LOCUS       DASYWL010000214         6324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_423064, whole genome shotgun sequence.
ACCESSION   DASYWL010000214 DASYWL010000000
VERSION     DASYWL010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6324
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423064"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1581)
                     /locus_tag="VGR22_07555"
     CDS             complement(97..1581)
                     /locus_tag="VGR22_07555"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2128456.1"
                     /translation="MARSHPRLLERLPNNRHPEQAAADASPLGSALGTAEQRARVSER
                     QRQFRSIRRRGRNGASVIAEPSLLNDADFRMLWLSRLLSQTAQGALLYAMLILVVDLS
                     DRTIYSSLFVLCSIIPSMLFGLPAGVVVDAVPRRVLLVILNLLRFTFMLFIVSAAEPS
                     LGDVFAATLGIWVIHQFYAPAEASLMGAIVPSARYTSAQALFNLALTIAQALGLVLLA
                     PLLLRAGGPRTVFAAAGLLFMVAGLLTVLLPAVGRAEQRLQRRHRSIRDILGEGWRFA
                     KGDRPTFEAILDDVLVAIGMSALVVIVPFYLERVLNTSKENTVFVFAPAALGLIFGLR
                     LAPVLGRLFGERLVATGSLFIFAASVGALGFVEQLHDLLTGLLHLPLDQISDLLSISP
                     LILVAMLISIPAGFASALVNVAARSILLSRTPERVRGQVIATQGLMGNVGALFPTLLA
                     GIATDIFGVQPIAVAIALATMLVALAAHAWGRRPPVPVETASLA"
     gene            complement(1668..2216)
                     /locus_tag="VGR22_07560"
     CDS             complement(1668..2216)
                     /locus_tag="VGR22_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128457.1"
                     /translation="MPSVTLISFDWLPLGPFDSRLWWLLIALFIALLFTIQAVEWAIE
                     GAWPHQRRPSSFLPRARAVQTGWAVVALLIIPGGLLAIANVAAVMWQELEHTDAQVLG
                     GWLLGVSWIVFLLGTRNTLGFTRLLNVIGVSGTIVLVLLLLIANILLLSSLLDILPDW
                     ETIEQGIEDGIERLIPFLGDDE"
     gene            complement(2461..3378)
                     /locus_tag="VGR22_07565"
     CDS             complement(2461..3378)
                     /locus_tag="VGR22_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2128458.1"
                     /translation="MAASDTKTLAANTEAEEREHQARSPKGRGLWSGVLRQKLAMIGL
                     VIVGFFAIAAVVGPLVAPSGPTEQFPEYRLQGPSSEYWFGNDEFGRDIFSRLLYGARI
                     SFQVGVIAVGISALIGIMIGMLAGYFGGWLDNIATLLMDVLYSFPTILLAIAIMAMLG
                     NSLANVMIAIGIVNAPTFMRVIRGAVLSVRRTTYVEAAISVGSPTSRVMMRHVLPNVT
                     APLIVHASLNFAYAVLAEASLAFLGLGNRPPSPSWGSMVSSSYGFLQLAPWAAIFPGV
                     TIALTVLGFNLLGDGLRDALDPRLRNEHG"
     gene            complement(3427..4377)
                     /locus_tag="VGR22_07570"
     CDS             complement(3427..4377)
                     /locus_tag="VGR22_07570"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2128459.1"
                     /translation="MLKFIINRVLLMIPILLGVSLVTFIIVRSIPGDPVRVLIGMDQR
                     ATPEQIDNIRRAYGLDQPLPVQYLRWMSHVLQGDLGSSLRTTRPLTTELGLRLPVTAE
                     LTVLAAVLGTIPALVFGVLAALRRNTRFDWLTTAFTMIGISVPNFLLATVLVLLFSFR
                     LKWLPPVGYTPFTEDPIQNLKTMVLPAISLAMPFMAIMMRFTRSSVLEVIGQEYVRVA
                     RAKGLPQRRVLLRHILPNAGIPILTIAGIQVAALLGGTVIVEQIFGLPGVGRYIYEAI
                     ANRDYPVVQSVVLVMATIFVMVSLVVDVLYAVLDPRLRTG"
     gene            complement(4542..6185)
                     /locus_tag="VGR22_07575"
     CDS             complement(4542..6185)
                     /locus_tag="VGR22_07575"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2128460.1"
                     /translation="MAKIRTIGSEMQTVRRLNNAIRTGRLTRREAMLRGGALGLSLPA
                     MMALHGTGGALGVSVASRQDSEPVSGGTLTLGVQADPAELDPHKTSLTAAWHVIEHVY
                     ETLITTDETLSPVPALAESWEISDDGLTYTFNLREGVFFHNGREFVADDVKYSVERIL
                     DPETASPVVDELASVESVEAVDTYTAVITLKNPDASFLAKLMGSSIAIVPQEVVEERG
                     DLMQTMVGTGPFRFLDYVPNTEVRLERFADYWDAPLPYLDALNIQIIPDNTARTTALV
                     QGTADIIEYAPVQDLPIFEADDTIVVTGNENTNIRYMGINVQREPFDQLEVRQAISMA
                     IDRGPIIDAAVFGYGTPTNVIFPASYWAGIESEIPEPDIEGARALLESVGLGDGFECQ
                     IQSWAQYPFLSNAAIVIQEQLRQIGIEGEVDLQENAVMLENYFSGNFDLSVTGTSAYV
                     DPNDVIQSNFGTDQASNGEGYSNPEVDRLIQEGIGTTDQEERAEIYAEIQRILLNDLP
                     WINLFIAEQYEAMKDYVQGYVHIPTGSNRSLKSVWIAEE"
BASE COUNT         1204 a         2024 c         2092 g         1004 t
ORIGIN      
        1 cccgcaccgt tcgtgctcct tgtttcggtc ggcaatgtcg agcccgtcat cactatcgtt
       61 cccggcgaga tcgcaacggt gtccgattga cagaggtcac gccagcgagg ccgtctcgac
      121 cggcaccggc gggcggcggc cccaagcgtg cgcggccagc gccaccagca tcgtcgccag
      181 cgcgatcgcg acggcgattg gttgcacgcc gaagatatcc gtcgcgatac ccgccagcag
      241 cgtcgggaac agagctccga cgttccccat cagcccctgc gtcgcgatca cctgcccgcg
      301 cactcgctcg ggtgtccgcg agagtaggat cgagcgcgcc gccacattga cgagcgccga
      361 ggcgaatccg gccgggatcg agatcagcat cgccacgaga atcagcggcg agatgctgag
      421 gaggtcggag atctggtcga gcgggaggtg cagcagcccg gtcaggaggt cgtgcagctg
      481 ttccacgaag cccagtgcgc ccacgctggc cgcgaagatg aacagcgatc ccgtcgcgac
      541 cagccgctcg ccgaagagtc tgccgagaac gggcgccagc cgcaggccga aaatgagacc
      601 gagcgccgcc ggcgcgaaga cgaagaccgt attctccttc gaggtgttca gcacccgctc
      661 aaggtagaac ggcacgatta ccaccagtgc gctcatgccg atcgcaacca gcacatcgtc
      721 gaggatcgcc tcgaaggtcg gccgatcgcc cttggcgaag cgccacccct cgccgaggat
      781 gtcgcgaatg ctccgatgtc gccgctggag ccgctgctcg gcgcgaccga ctgccggcag
      841 cagcaccgtg agcaacccgg cgaccatgaa cagcagcccc gcagcggcga agacggtgcg
      901 cggcccgccg gcgcggagca gcaacggcgc cagcagcacc agcccgagcg cctgggcgat
      961 ggtcagcgcg aggttgaaga gcgcctgggc cgaggtatac ctcgccgagg gcacgatcgc
     1021 ccccatcagc gacgcctcgg cgggcgcata gaactggtgg atcacccaga tgccgagggt
     1081 cgcggcgaag acgtcgccga ggctcggctc ggcagccgag acgatgaaga gcatgaacgt
     1141 gaatcgcagc aggttgagga tcaccagcag cacgcgacgc ggtacggcgt cgaccacgac
     1201 tcctgccgga aggccgaaga gcatcgaggg gatgatcgag cagagcacga agagcgagct
     1261 gtagatggtg cggtcggaga gatcgaccac cagaatcagc atcgcgtaga gcagcgcgcc
     1321 ctgagcagtt tgcgagagca ggcgcgacaa ccagagcatg cggaaatcgg cgtcgttcag
     1381 cagcgatggc tctgcgatga cggaggctcc attccggccg cgacggcgga tggagcggaa
     1441 ttggcgttgg cgctcggata ccctggcgcg ctgctcggcc gtgccgagtg cgctgccgag
     1501 cggagacgcg tcagccgcgg cctgctcagg atgccggttg tttgggagcc gttcgagcaa
     1561 ccgggggtgc gatctggcca accggtggcg actccgcgtc actggcaccg acaggcgtcg
     1621 gctcggtcgc tgcaccgggg ggattcgacg tcgcatctcg ccacgcatca ctcgtcgtct
     1681 cccaggaacg gaatcagccg ttcgatgcca tcctcgatgc cctgctcgat cgtttcccaa
     1741 tccgggagga tatccaacag tgacgagagc agcaggatgt tggcgataag gagcagcagc
     1801 acgagcacga tagttcccga aactccgatt acgttcagca gacgggtgaa gccgagcgtg
     1861 ttgcgggtac cgagaaggaa gacgatccag ctcactccga gcagccagcc gccgagcact
     1921 tgggcatcag tatgctccag ctcctgccac atcaccgcgg cgacgttggc gatcgccagc
     1981 agtccgcccg ggatgatcag cagcgcgacc accgcccagc cagtctgcac ggcacgagca
     2041 cgcggcagga aactggaagg gcggcgctgg tgtggccatg cgccctcgat tgcccattcc
     2101 acggcttgga tggtgaacag gagcgcgatg aagagggcga tgagcagcca ccataaccgg
     2161 gaatcgaacg gcccgagggg aagccagtcg aacgagatga gcgtcactga aggcacaacc
     2221 ggtttcatcc tggctgcggc cgcatcacca tgtcgcgccg cggccgtcgt cagggcggac
     2281 gaaatcgcca cgcgacaagt atacgagggt atcggccacc accggggcag tggacgttcc
     2341 atacgcattc ggggtcgcaa gatgcgggcc cggattgtac cccgggcaag atcggaacgg
     2401 ctcggcttcg gagaggcggg gctgtcagtc tgcggattcg cgacagattt actcacaccg
     2461 ctatccgtgc tcgttcctca agcgggggtc gagggcatcg cggaggccgt cgccgaggag
     2521 gttgaagccg aggacggtca gggcgatggt gacgccggga aagatcgccg cccacggcgc
     2581 caactggagg aagccgtagg agctggagac catcgacccc catgatggcg acggcggccg
     2641 gttgccgagt ccgaggaagg ccagcgacgc ctctgccagc accgcgtagg cgaagttgag
     2701 cgaggcgtgg acgatgagcg gcgcggtgac gttcggcagc acgtgccgca tcatcactcg
     2761 cgaggtcggg ctgccgaccg agatcgccgc ctccacatag gtcgtccgac gcacagagag
     2821 caccgcgccg cggatcacgc gcatgaacgt cggcgcattg acgatgccga tcgcgatcat
     2881 cacgttcgcc agcgagttgc ccagcattgc catgatcgcg atcgccagca ggatggtcgg
     2941 gaacgagtag agcacatcca tcagcagggt ggcgatgttg tcgagccagc ccccaaagta
     3001 gcctgccagc ataccgatca tgatgccgat cagcgccgag atgccgactg cgatcacgcc
     3061 cacttggaag gagatgcggg cgccatagag caatcggctg aagatgtcgc ggccgaactc
     3121 gtcgttgccg aaccagtact cgctgctcgg gccctgtaat cggtactcgg ggaactgctc
     3181 ggtcggccca ctcggtgcga cgagcgggcc gacgaccgcc gcgatcgcaa agaacccgac
     3241 gatcaccagc ccgatcatgg ccaacttttg ccgcagcacg ccgctccaga gcccacgtcc
     3301 cttggggctc cgcgcctggt gttcacgctc ttcggcctcg gtgttggccg ccaaggtctt
     3361 ggtgtcactc gccgccatgg tatccggtcc tcgttacacc gcgtcctgcc acctgcatca
     3421 accggctcat cctgtgcgca gccggggatc gaggacggca tagagcacgt ccaccaccag
     3481 gctcaccatc acgaagatcg tggccatcac cagcaccacg ctctgcacca ccgggtagtc
     3541 gcggttggcg atcgcctcat agatgtatcg cccgacgccg gggagtccga agatctgctc
     3601 gacgatcact gtgccgccca gcagcgccgc cacctggata ccggcgatcg tcaggatggg
     3661 gatgcccgcg ttcggcagga tgtgccgcag cagcacccgt cgctggggca gccctttggc
     3721 gcgcgccacg cggacatact cctgcccgat cacctccagc acggacgagc gcgtgaagcg
     3781 catcatgatc gccatgaagg gcatcgccag gctgatcgcc ggcaggacca tcgtcttgag
     3841 gttctgaatc gggtcctcgg tgaagggagt gtaccccacc ggtggcagcc acttgagcct
     3901 gaacgagaag agcagcacca gcaccgtcgc caataggaag ttcggcaccg agatgccgat
     3961 catcgtaaac gcggtcgtca gccagtcgaa gcgggtattg cgccgaagcg cggccagcac
     4021 cccgaagacc agggccggga tcgtgccgag cactgccgcg aggacggtca gctcggcggt
     4081 caccggcagc cgcaacccga gttcggtggt cagcgggcgg gtggtacgca gcgacgaccc
     4141 cagatcaccc tgcagcacat ggctcatcca gcggagatac tgcaccggca gcggctggtc
     4201 gagcccgtag gcacggcgga tgttgtcgat ctgctcagga gtcgcccgct gatccatgcc
     4261 gatcaacacg cgcaccgggt caccggggat cgaccgcacg atgatgaagg tgaccagcga
     4321 gaccccgagc aggatcggga tcatgagcag cacccggttg atgatgaact tcagcatgcg
     4381 gcggtcctgc ctctcacgga atcaggaagt tgtgtctgtc aaggtagcga tctgacctcc
     4441 cgcagcccac tgaggttgac ggcacagaaa gcatggggtc ggggatgatg cgcccccgac
     4501 cccatgccat gatcagccag cgacgcggga cgctacaacc gctactcctc ggcgatccag
     4561 accgacttga gcgagcggtt ggagcccgtc ggaatgtgga cgtagccctg gacgtaatcc
     4621 ttcatcgctt cgtactgctc ggcgatgaac aggttgatcc acggcaggtc gttgagcagg
     4681 atgcgctgga tctctgcgta gatttcggct cgctcctcct ggtccgtggt gccgatgccc
     4741 tcctgaatga ggcggtcgac ctccggattg ctgtatccct cgccattgga ggcctgatcc
     4801 gtgccgaagt tgctctgaat cacgtcattc gggtcgacgt aggcgctggt accggtgacc
     4861 gacaggtcga agttgccgct gaagtagttc tccagcatca ccgcgttctc ctggaggtca
     4921 acctcgccct cgatgccgat ctggcggagc tgctcctgaa taacgatcgc ggcgttcgag
     4981 aggaaggggt actgcgccca cgactggatc tggcactcga agccgtcgcc caacccgacc
     5041 gactccagca acgcccgcgc gccttcgatg tccggctcag ggatctccga ctcgatgccg
     5101 gcccagtagc tcgccgggaa gatcacgttg gtcggcgtgc cgtagccgaa caccgccgcg
     5161 tcgatgatcg ggccgcggtc gatcgccatc gagatcgcct ggcgcacctc gagctggtcg
     5221 aacggctcgc gctggacatt gatgcccatg tagcggatgt tggtgttctc gttgccggtg
     5281 acgacgatcg tatcgtcggc ctcgaagatc ggcaggtcct gcaccggcgc gtactcgatg
     5341 atgtcggccg tcccctgcac cagcgccgtc gtgcgggcgg tgttgtccgg aatgatctgg
     5401 atgttgagag cgtcgaggta cggcagcggc gcgtcccagt agtccgcgaa gcgctcgagc
     5461 cggacctcgg tgttgggcac gtagtcgagg aagcggaagg ggccagtgcc gaccatcgtc
     5521 tgcatcaggt cgccgcgctc ctcgaccacc tcctgcggca cgatggcgat cgacgatccc
     5581 atcagcttgg cgaggaagga agcatccggg ttcttcagcg tgatgacggc ggtgtaggtg
     5641 tctacagcct cgactgactc caccgaggcg agttcgtcga ccacgggcga ggccgtttcc
     5701 ggatcgagga tgcgctcgac gctgtatttg acgtcgtcgg ccacgaactc gcgtccatta
     5761 tgaaagaaca cgccctcacg gagattgaag gtgtaggtca ggccgtcgtc ggagatctcc
     5821 cagctttcag ccagggctgg caccggggag agggtctcgt cggtggtgat gagcgtctcg
     5881 tagacgtgct cgatcacgtg ccaggccgcc gttagcgagg tcttgtgcgg gtcgagctcg
     5941 gctgggtcgg cctgcacgcc gagggtgagg gtgccgccgg aaactggttc gctgtcctgc
     6001 cgcgacgcca cggagacgcc gagtgcgccg ccagtgccat gaagcgccat catcgcgggc
     6061 agcgagagcc caagggcgcc gccccgaagc atcgcctcgc gtcgcgtgag ccgcccggtt
     6121 cgaattgcat tgttcagccg ccgaactgtc tgcatctcgg aaccgatggt acgaatcttc
     6181 gccattgtcc tgatacctcc gatagaaatg gaatgtcgtg cacaccggcc tcaccaacgg
     6241 ctgcagaggg ctcctgctgt ccgcattggc atacgggtcg gatcagggca tgcccgatgg
     6301 aacgtccgcg cacgacccga taca
//
LOCUS       DASYWL010000215         1913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_234710, whole genome shotgun sequence.
ACCESSION   DASYWL010000215 DASYWL010000000
VERSION     DASYWL010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234710"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VGR22_07580"
     CDS             <1..181
                     /locus_tag="VGR22_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128461.1"
                     /translation="TTGAWPGCQSFCGWKGSGSTGKGGLGKFYVQQFLREQSQTRVTE
                     PADAAEVESVEARGE"
     gene            complement(126..1733)
                     /locus_tag="VGR22_07585"
     CDS             complement(126..1733)
                     /locus_tag="VGR22_07585"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2128462.1"
                     /translation="MAQQENFDAIVIGAGQGGGPIASSFARDGRRTALIEREHAGGSY
                     INWGCTPTKTMIASGRVAHVARRARGYGVHTGELRIDMERVRKRDVVQMFRDASRASI
                     EGTDGLEYIKKGEARFTGEKTIEVALDNGGNRSMRAETFVLDVGQRARPLDLDIPAGV
                     TILDNRSVMELDEVPEHLLVVGGGYVGIEFGQLFRRLGAEVTIVHRSDHLLSHEDPDV
                     AEAVAEILREDGIALEMHTRPTGVTSRGEGIEVTIERSEDPPGTVFVSHVLAAAGRLP
                     NTDSLDVEQTGLKLDDRGQIATDDRLETAVTGIYAIGDIRAGPKFTHISYDDYRILRA
                     NLIDGGNRSVDDRPVPYTTFIDPQLGRIGLTERHAQEQGIPYQVGRLQMSSVARAIET
                     DETRGMMKALVHAETGRILGAAVLGIEGGRNRGDAPDHHDGQSALHRPARRCFRPPDA
                     RRVPQQRLRESAGPRVIADPGLRSCTRTTATWIRCSARETIAAVDAETTAAMVVTPLP
                     ASTAVDRRTSTRRALRPTQLPQRQQAP"
BASE COUNT          307 a          610 c          635 g          361 t
ORIGIN      
        1 gaccaccggc gcgtggcccg gctgccagtc cttctgtggc tggaagggga gtggctcgac
       61 cggaaagggt gggcttggca agttctacgt gcagcagttc ctgcgggaac agagccagac
      121 acgggtcacg gagcctgctg acgctgcgga agttgagtcg gtcgaagcgc gcggcgagta
      181 gacgtgcgtc gatcaacggc ggtgctggca ggtagcggtg tgaccaccat cgctgccgtg
      241 gtttctgcat ccacggcggc gatggtttcc cgggcggaac accgaatcca agttgccgtt
      301 gttcgcgtgc aggaacggag gccgggatcc gcgattactc ggggtcctgc agattcgcga
      361 agacgttgtt gagggactct gcgagcgtcg ggtgggcgaa aacaccgtcg cgcagggcgg
      421 tgtagggcag attgcccatc atggtgatct ggagcgtcgc cgcgatttcg ccccccctcg
      481 atgccgagca ccgctgctcc gaggatgcgt cccgtttcgg catgtaccag cgccttcatc
      541 atcccgcgcg tttcgtccgt ctcgatcgcc cgcgcgacgc tgctcatctg caaccttccg
      601 acctgatagg ggatgccctg ctcctgcgca tgcctctcgg tgagcccaat gcggccgagc
      661 tgcgggtcga tgaaggtcgt gtaggggacg gggcggtcgt ccacggagcg gttgccaccg
      721 tcgatcagat tcgctctgag aatgcggtag tcgtcgtagg agatatgggt gaatttcggg
      781 ccggcccgga tgtccccaat cgcgtaaatc cccgtcactg cggtctccaa ccggtcgtcg
      841 gtcgcgatct gcccccggtc gtccagcttg aggccggtct gctcgacgtc gagtgagtcg
      901 gtgttcggca atcggccagc ggcggccagc acatgggaaa cgaatacggt tcctggcgga
      961 tcctcgctgc gttcgatagt cacctcgatg ccttcgccac gtgaggtgac gccggtcggc
     1021 cgggtgtgca tctccaaggc gatgccgtcc tcacgcagga tctctgcgac ggcttcggcg
     1081 acgtctggat cctcatgaga gagcagatgg tcgctccgat gcacaatggt tacctcggca
     1141 ccgagacgcc ggaagagttg gccgaactcg atcccgacgt agccgccgcc gaccaccagc
     1201 aggtgttccg gcacctcatc cagctccatg accgaacggt tgtcgaggat ggtgacaccg
     1261 gctgggatgt cgaggtcgag aggccgagcg cgctggccca cgtcaaggac aaaggtctcc
     1321 gcgcgcatcg accggttgcc gccgttgtcg agcgccacct cgatggtctt ctcgccggtg
     1381 aagcgcgcct cgcccttctt gatgtactcc agaccatctg tcccctcgat gctcgcgcgg
     1441 ctggcgtcgc gaaacatctg gacgacgtcg cgcttgcgca ctcgctccat gtcgatgcgc
     1501 agctccccag tgtggacgcc atagccccgg gcgcgacggg caacgtgagc gacacgcccc
     1561 gaggcgatca tcgtcttcgt cggcgtgcag ccccagttga tgtagctgcc gccagcgtgc
     1621 tcgcgctcga tgagtgccgt cctgcggccg tcgcgagcga aggagctggc aatggggccg
     1681 ccgccctgac cggcaccgat cacgatcgca tcgaagttct cctgttgcgc catgtgtcac
     1741 tcccgacgtg ttacccgatg ccggcctgga ccgctccatg ctacggtgag gccgaacgga
     1801 ggagcccgat gtcgatgttc tgccagcgct gtggcgcgcc aactcacgat gtctgccggg
     1861 atggtcgcat gcgaccggtc tgcacgtcat gcggaacgac gacctacctt gac
//
LOCUS       DASYWL010000216         9189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_648223, whole genome shotgun sequence.
ACCESSION   DASYWL010000216 DASYWL010000000
VERSION     DASYWL010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9189
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648223"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..497)
                     /locus_tag="VGR22_07590"
     CDS             complement(69..497)
                     /locus_tag="VGR22_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128463.1"
                     /translation="MSTTPILKKSKRAIAITGIALSALVLAACGDDEAAEVVEEVDEV
                     ATEVVEEEEVVEEEVAEEEVVAEDEAAEEVVGEVGTPEEEEVVASPVAGATPVPADAD
                     AAPATPAAATPAPVGATPVPADVDAAPATPVASPMATPEA"
     gene            701..1591
                     /locus_tag="VGR22_07595"
     CDS             701..1591
                     /locus_tag="VGR22_07595"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEV2128464.1"
                     /translation="MPTLPADHPWAHELHAIVPLVREVATAVRGIYEAQGAVTYTKED
                     GSPVTDADLTADRMLRAGIQQAFPADALLTEESEDDLARLGNPRCWIADPIDGTAQFV
                     ARTGLFDILLALVVEGHPVVSICAHPPSGVMTLAVRDHGAWSIDERGEIAPFRFEPPE
                     SPPRLATSRYYGPVDFADAIEHITAELGAPPVPVLAFGYGPRAFLPRWRTYDAFIGFW
                     KRAGDSPVREWDLAASDLMTTESGGAFTDMYGNQYRYNQPSPRPRAGVLASASPELHQ
                     RILTVVAPCLPTTPPPVLTR"
     gene            complement(1592..2338)
                     /locus_tag="VGR22_07600"
     CDS             complement(1592..2338)
                     /locus_tag="VGR22_07600"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEV2128465.1"
                     /translation="MAGYVLEVIESYEEMSVRAADAVQETVERRPDAAITVPTGQTPL
                     GMYEDLVRRVEAGTIDLSQVRIFCLDDYLGQSRHDEASLTRWLYEAFLIPAGIPESHI
                     HRIPAAAPDPHAAALAYEREIAALGGLELAVIGLGQNGHVAFNEPGSAPDSRTRVVEL
                     TGESREQSSAYWEGEAGIPPYAITMGLGTIQEARQIVLIVSGEAKAGIVHQALEEEPK
                     LEVPASWLQTVGERLHVILDREATSELTPR"
     gene            2546..3667
                     /locus_tag="VGR22_07605"
     CDS             2546..3667
                     /locus_tag="VGR22_07605"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEV2128466.1"
                     /translation="MPTIQEALDTIDQWIDDGAVPGAAAAVWHRGEIIAAHTTGEATP
                     GRHVTNDTIFALASVSKPMTAAAVMQVLDREDVDLDTPLAEILPEFESVEDPFDEDLL
                     PQLEAQRHEVTMRQLLCHTSGLPENVGVKRIRMTALPSLGEIVDVMCNVPLQSPPGET
                     LRYSNVGLALAARAASRLADRDFHSMLQEDILDPAGLDGIVLRPGPKLDDRVAHVDDA
                     AGNGGKSESYNSRYWRDMGIPWGGYFGTALDVLRFATSFLPGQASPLRDDLKAQMVVD
                     NTGGVPGGVNSAGFHWEEGAWGLGWEVKGSKRNHPTGTLTSEGTWCHWGQSGTMVWVD
                     PARELGLSVFSNRTVMKGWPLRPPRWSTLSDAVISAVGP"
     gene            3639..4856
                     /locus_tag="VGR22_07610"
     CDS             3639..4856
                     /locus_tag="VGR22_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128467.1"
                     /translation="MPSSAQWDREMSSRLTTSGAQPRYLGLAACAIILVVTTIAMPLW
                     TNAASFRADTDVTIPAAEVVEEDLFVASDTFLLDGTAEQDVFAGARSVEIDGTVEDSL
                     NVVSGETTISGSIAHSVRVAGGMVNVSGTIGGDLVVLSGFTTIAEGATIDGDVHVFGG
                     MLTVNGAVGDDVTGTIGVLEIDGDIAGDVRADVERLDLGNDAAIAGELIYISPVEADI
                     TPGVEVAGDFDRQSAAPWGMESGPRARFFSPLARTVWLLVAGAFIIAIAPRLATALNE
                     TLDRPLPAAIAGLLALVSVPVLAILLLATVVGIPLGLLLLAGYIVALYLSQVVVGQRL
                     GAWLLPNRWADGSRGHLLLSMTLGVLLLSALRYVPVPFFDTVVNMLVAVAGLGAAVLL
                     FGRLRPKRAVDRV"
     gene            complement(4949..5389)
                     /locus_tag="VGR22_07615"
     CDS             complement(4949..5389)
                     /locus_tag="VGR22_07615"
                     /inference="COORDINATES: protein motif:HMM:NF024940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2128468.1"
                     /translation="MRGRPLHITWEESAEELKEMLDKERDPRRRSRLQAFCMLRQGKR
                     ISDVTDVVGADYRTVQRWVAWYRAGGLDSVLRRTPGHAAPGRRSKLTAQQTQVLLDEY
                     EAGRFRSIREAVDWASDTFGVDFTYTGMHAHLRRAQRRASASSA"
     gene            5889..6620
                     /locus_tag="VGR22_07620"
     CDS             5889..6620
                     /locus_tag="VGR22_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128469.1"
                     /translation="MTGAPITDPNPLALAAAGFLAQALELRDNPAAIMNDLTEMKADP
                     NAAIYSIEVDSSIGVAAFLVYVYVMQERNEDGLTGDELYRQGLATLQQATDRDTPGPR
                     MVAHAETETVAFILATTPATWRAMQGESPPSMVATEGDLLMAARTEEERAAIAEDLHR
                     SLKEANDHARTWLAAISAAGSSSDADVLTFTEAETALALFVLDSANVEPTLNALNLLV
                     TGAQEQAGKMISRRQAAAPDRAGEE"
     gene            complement(6657..7154)
                     /locus_tag="VGR22_07625"
     CDS             complement(6657..7154)
                     /locus_tag="VGR22_07625"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV2128470.1"
                     /translation="MATADDGRINVDMEHNCFGCGDLSAHGLHLSFYPDEGGGVWTAF
                     VPAERFEGYTGMVHGGVISTVLDEVMAWSLYRNEIWAVTVSLSVRYRAPIRVRERTRA
                     VGWVVRRRGRRIEAAGEIRRAEDGKALASATATFVQVPEPQAREWRERYLGDGNRHAE
                     VAGKG"
     gene            complement(7211..8041)
                     /locus_tag="VGR22_07630"
     CDS             complement(7211..8041)
                     /locus_tag="VGR22_07630"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HEV2128471.1"
                     /translation="MTDEFPYDSAATDSPADAALLERLQPVAQLLAPSRHMVVFTGAG
                     ISTESGIPDYRGPNGVWKTNRIPTADSVPADRASREDRWQSHRKRYPEMQARDPNAGH
                     RAIAALERGGRVSAVITQNIDGLHQKAGSDPARVLELHGSSHRLRCVDCGRIYDTAEI
                     VERLEQGERDPRCVVCGGVLRSSTVLFGESLPEETLRKAIEASASADVMLVVGSSLVV
                     KPAAQLPAVARKRGASLVIVNREPTPLDDVANAVVRGEAGPVLQALAHLVINGSDARP
                     "
     gene            complement(8191..8358)
                     /locus_tag="VGR22_07635"
     CDS             complement(8191..8358)
                     /locus_tag="VGR22_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128472.1"
                     /translation="MDPERRAVSTSSVLGGTVAGSPLHRIAVEHGLLLYSVRAVESGM
                     TSDELAQPFVC"
     gene            8457..9050
                     /locus_tag="VGR22_07640"
     CDS             8457..9050
                     /locus_tag="VGR22_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128473.1"
                     /translation="MTDMAVRDIEARRASLLGSLRNLLLAVFGRESDHGLPLGIKHRQ
                     DRTFGSAMRPVDRPTPRVVQEQQLATPPPSGEHRWERHPQLRSRQAGPVPTGSTRSVR
                     LEAERGLILARAGKLVEAVEAFTVAAGDPEIDLAALPGFWDLPRNGMLTAVRAYERAE
                     RLRDAATLEARIRHLLKPRSVRTTPATRPGRLAASGD"
BASE COUNT         1554 a         3227 c         2838 g         1570 t
ORIGIN      
        1 cggttcgtcg gcacccgcgt cgctcgttct ctgtcaccac ggtatcgccg gtaaggcagg
       61 tggatcggct aggcctccgg tgtcgccatc ggagacgcga ccggggttgc aggtgccgca
      121 tccacgtcag cgggaactgg cgtcgcgccg acgggagccg gggtcgcagc ggcgggggtt
      181 gccggtgccg catccgcatc ggcaggcact ggcgtcgcac cagcgactgg cgaggcaacc
      241 acctcctcct cctcgggagt gccgacctcg ccgacaacct cttcggccgc ctcgtcctcg
      301 gcaacgacct cttcctcggc tacctcttcc tcgacaacct cttcctcctc gacgacctcg
      361 gtggcgactt cgtcgacctc ctcgaccacc tcagcagcct cgtcgtcgcc gcaagccgcg
      421 agcaccagtg cgctcagcgc gatgccggta atggcaatgg ctcgcttgct cttcttgaga
      481 atcggtgtcg tgctcatcgt tcgtgtttcc cctccgtagc cgcggttcga tcgtgggctt
      541 caaaccgtcc tcagatctcg ctgttgatgt acataccaca accctcggtg acggggtacc
      601 ctattcgggg ctacgcccgg gccgaggaag ccaactcatg ccgtggcaag cgagatccgc
      661 taaccccaga ctctaacagt atgccgtaaa ggattctgtc atgccaacac ttccggcaga
      721 ccacccttgg gctcacgagc tgcacgccat cgttcccctc gtccgggagg tcgcgaccgc
      781 cgtgcgcggc atctacgaag cgcagggagc ggtgacctac accaaggaag acggctcgcc
      841 ggtaactgat gccgatctca ccgccgatcg gatgctccgc gcgggcatcc agcaggcatt
      901 ccctgctgat gcgctgctga ccgaggaaag cgaagatgac ctggcccgtc tgggcaaccc
      961 gcgctgctgg atcgccgacc ctatcgacgg cacggcccag ttcgtcgcgc ggaccggcct
     1021 gttcgatatc ctgctggcgc tggtcgtcga ggggcacccc gtcgtgtcaa tctgcgcgca
     1081 cccgccaagt ggggtgatga cactggcggt gcgtgaccac ggcgcctggt cgatcgacga
     1141 gcgcggcgag attgccccgt tccgcttcga gccaccggaa agccctccga ggctcgccac
     1201 ctcacgctac tacggcccgg tcgacttcgc cgacgcgatc gagcacatca ccgcggagct
     1261 cggtgcccca ccggtgccgg tgctcgcgtt tggctacggt ccccgcgcgt tcctgccgag
     1321 atggcggacc tacgacgcct tcatcggatt ctggaagcgg gccggcgatt cgccggtgcg
     1381 cgagtgggac ctcgccgcga gcgacctgat gacgaccgaa tccggcggcg ccttcaccga
     1441 catgtacggc aaccagtatc gctacaacca gccgtctcca cggccgcggg ccggcgtcct
     1501 ggccagcgcc tcgccggaac tccaccagcg catcctcacg gttgtcgcac cctgcctgcc
     1561 caccaccccg cctccggtcc tgacccggtg atcatcgtgg agtcagttcg ctggtggcct
     1621 cgcggtcaag aatgacgtgc aatcgctcgc ccacggtctg cagccatgac gccggcacct
     1681 ccagtttcgg ctcctcctcc agcgcctggt gaacgatccc cgccttcgcc tctccggaga
     1741 cgatcagcac gatctgccgc gcctcctgga tcgtaccgag gcccatggtg atcgcatacg
     1801 gcggaatccc cgcctcgccc tcccagtagg cagacgactg ctctcgtgac tccccggtca
     1861 actcgaccac gcgcgtgcgg ctgtcggggg cggatcccgg ctcgttgaag gcgacgtgcc
     1921 cgttctgccc gagtccgatc accgccagtt cgagcccacc cagcgcagcg atctcccgct
     1981 catacgccag cgccgcggcg tgaggatccg gcgcggcagc aggtatgcga tggatatggc
     2041 tctcagggat gccggcgggt atcaggaacg cctcgtagag ccaccgggtg aggctcgcct
     2101 cgtcgtgccg gctctggccg aggtagtcat cgaggcagaa gatgcggacc tggctcaggt
     2161 cgatcgtgcc ggcctcgacc cgccgtacca gatcctcgta cattcccaaa ggcgtctggc
     2221 cagtcggtac cgtgatcgcg gcatctggcc gccgctcgac cgtctcttgg accgcatccg
     2281 ctgctcggac actcatctcc tcgtagctct cgatcacctc caaaacgtat cccgccatat
     2341 cgtcctcctc atcccgatgc tgcgccgctg cggtcagatg accgtgttgc cgacgcccta
     2401 gcttacccgc tcccggtcgc gctgccccct cgtatcccct acaatctctg gcgactctct
     2461 cgtatcgagc ggagcgtccg ggttcgtgaa cctcgcgctc aaactcgtca ccgacgccca
     2521 atccaggaag gacaccatct ccagcatgcc caccattcag gaggcgctcg acaccatcga
     2581 ccagtggatc gatgatggcg cggttcccgg tgccgccgcg gcggtctggc accggggcga
     2641 aatcatcgct gcgcacacca ccggcgaggc gacacccggc cgccacgtca ccaacgacac
     2701 catcttcgcg ctcgcgtccg tctccaagcc gatgacggcc gccgccgtca tgcaggtgct
     2761 cgacagggag gacgtcgacc ttgacacgcc actcgcggag atccttcccg agttcgagtc
     2821 ggtcgaagac ccgttcgacg aggatctcct gccacagctg gaggctcaac gccacgaagt
     2881 gacgatgcga cagctgctgt gtcatacctc gggattgccg gagaatgtcg gcgtcaagcg
     2941 catccgcatg acggcactcc cctcgcttgg cgagattgtc gacgtgatgt gcaacgtgcc
     3001 gctgcagtca ccgccaggag agacgctgcg ctactccaat gtcggattgg ctctcgccgc
     3061 gcgcgccgcc tcccgactcg ccgaccgcga cttccacagc atgctgcagg aggacatcct
     3121 tgacccggcc ggactggacg gcatcgtatt gcgcccaggt cccaagctcg acgaccgcgt
     3181 agcccatgtc gatgacgcgg ccggcaatgg cggaaaaagc gagagttaca acagccgcta
     3241 ctggcgcgac atgggcattc cctggggcgg ctacttcggc acggccctcg acgtgctgcg
     3301 ctttgccaca tccttcctgc ctggccaagc gagtccacta cgggacgatc tcaaggcgca
     3361 gatggtggtt gacaacaccg gtggcgtgcc gggcggcgtc aactcggctg gcttccactg
     3421 ggaagaggga gcatggggac tcggctggga ggtcaagggc agcaagcgca accacccgac
     3481 tggcacgctc acctcggagg gcacgtggtg ccactgggga cagtccggga cgatggtctg
     3541 ggtcgacccg gcgcgcgagc tcggcctctc cgtcttcagc aaccgcacgg tgatgaaggg
     3601 ttggcccctt cggccaccac gctggtcgac actgagcgat gccgtcatca gcgcagtggg
     3661 accgtgagat gtccagccgg ctgaccacct cgggcgctca gccccgctac ctcggccttg
     3721 ccgcctgcgc catcattctg gtggtcacga ccatcgcgat gccgctctgg accaatgcgg
     3781 cctccttccg cgccgacact gatgtgacga tcccggccgc ggaggtcgtc gaggaggacc
     3841 tgttcgtcgc cagcgatacc ttcctactcg acggaacggc tgagcaggac gtcttcgctg
     3901 gcgcccggtc cgttgagatt gacgggacgg ttgaggactc gctcaacgtg gtcagtggcg
     3961 aaacgaccat ctccggcagc attgcgcact cggtgcgcgt ggccggcggg atggtcaacg
     4021 tcagcgggac gatcggtggc gacctagtgg tgctcagtgg cttcaccacg atcgcggagg
     4081 gcgcgacaat cgatggtgac gtgcacgtct ttggcggcat gctgaccgtg aatggagcgg
     4141 tcggcgacga tgtcaccggc accatcggcg tgctggagat cgatggcgat atcgccggcg
     4201 acgtgcgcgc cgacgtggag cgactggacc tcggcaacga tgcggccatc gcgggcgagc
     4261 tcatctacat cagcccggtc gaggccgaca tcacgcccgg ggtggaggtt gccggcgatt
     4321 tcgaccggca atccgccgcc ccgtggggca tggaaagcgg accgcgggcc cggtttttca
     4381 gcccgctggc ccgcacggtt tggctgctgg tggccggtgc gttcatcatc gcgatcgcac
     4441 cacgcttggc gaccgcgctc aacgagacgc tcgaccgtcc cctgccggcg gccatcgcgg
     4501 ggctcctggc gctcgtgagc gtcccggtgc tggcaatcct gctgctggca acggtggtcg
     4561 gcatcccatt gggcctgttg ctgctagcgg gctacatcgt ggcgctctac ctgagccagg
     4621 ttgtcgtagg gcagcgcctc ggcgcctggc tgctgccaaa ccgctgggcc gacggcagcc
     4681 gaggccacct gctcctcagc atgaccctcg gggtgctgct gctcagcgcc ctgcgctacg
     4741 tgccggtccc cttcttcgac acggtcgtga acatgttggt agctgttgca gggctcggcg
     4801 ccgccgtact cttgttcggc cgcctccgac ccaagcgcgc cgtcgaccgc gtctagtgcc
     4861 ggaagttacc gcttctttgc gccaagcctg cgaccagggc aacacctgtt cggcattgcc
     4921 ccgaccgacg gaccatttgg gaacagcgtc aggcggatga cgctgacgcg cgtcgctggg
     4981 cgcgtcgcaa gtgcgcgtgc atacccgtgt aagtgaaatc gacgccgaac gtatcggacg
     5041 cccagtccac cgcctcccgg atgctccgga aacgtcctgc ctcgtattcg tcgaggagca
     5101 cctgggtttg ctgggctgtg agcttggagc gacgtcccgg tgctgcgtgc cctggagtgc
     5161 gacggagcac cgaatcgagt cctccggcac gataccaggc aacccaccgc tgcaccgtgc
     5221 ggtaatcagc cccgacgaca tccgtcacat cgctgatccg cttgccttgc cgcagcatac
     5281 agaaagcctg cagccgcgac cgtcggcgcg ggtcgcgctc cttgtcgagc atctccttga
     5341 gctcctcggc cgactcctcc caggttatgt gtaacggcct ccctctcatg acgcgagcct
     5401 ccttttccta cgccccctga ccagttcgac ggagcgtcaa ccgtccatca acacccggct
     5461 ccaaacacgc ctttcagcac agcacagcac ttgtccaacg agaccccggg aacgagtgcg
     5521 taccccgcgt gaagttgtcg gacggcccag tggacatatt atctgacaaa ttctaccaat
     5581 acgctacccc ctatgatgct cccaagtccc tacccccgag aggcaagcaa caacgattga
     5641 ggaagtgccg tgaattcggc tctgaatcag gcccgacgcc gacgatccgc gccgtgtcgc
     5701 cggtattccc tggatatttg ccggatggag attggacatc ctgaggccag ccgtaagtcg
     5761 ctctatcgag tcatcagcga ccacatcctc gcctcgcccc tcgatgcgtc gtaccctttc
     5821 ccgagcaccg cggccagcgt tcgctccgct gcccactcga ccactcgcga cgtcggcagg
     5881 atccaaaatt gacaggcgca cccatcacag acccgaaccc gctcgctctc gccgctgccg
     5941 gcttcctggc acaggcgctg gagctgcgcg acaacccggc cgcgatcatg aatgacctca
     6001 ccgagatgaa ggccgacccg aatgcggcga tctactcgat cgaggttgac tcgtccatcg
     6061 gcgtcgccgc cttcctcgtc tacgtctacg tgatgcagga gagaaacgag gacggcttga
     6121 ccggtgacga gctctaccgg cagggacttg cgacgctgca gcaggcgacg gatcgcgaca
     6181 cacccggacc gcgaatggtt gcccatgccg agacggagac ggtggcgttc atcctcgcaa
     6241 cgaccccggc aacatggcgc gccatgcagg gcgagtcacc accgtcgatg gtggccaccg
     6301 aaggggactt gctgatggcg gcacggaccg aggaggagcg cgccgcgata gccgaggatc
     6361 tgcaccgctc cctgaaggag gccaacgacc atgcaaggac atggctggcg gcgatcagcg
     6421 cggctgggag cagcagtgat gcggacgtgc tgacattcac cgaggcggag acggcactcg
     6481 cgctcttcgt gctcgacagc gcgaacgtgg agccaaccct gaacgcactg aacctcttgg
     6541 tgaccggcgc ccaagagcag gcaggcaaga tgatctctcg ccgccaggcc gccgctcccg
     6601 accgcgccgg ggaggaatag ccggtccggg tggaattcgg ggcagaccat ccctcgtcac
     6661 cctttcccgg ccacctcggc gtgccgattc ccatcaccga gatagcgctc gcgccactcc
     6721 ctagcctgcg gttcgggtac ctgcacgaag gtggccgtcg ccgaggcaag cgccttgcca
     6781 tcctctgccc ggcgaatctc gcccgcagcc tcgatccggc gaccacgccg ccggactacc
     6841 cagccgaccg cccgtgttcg ctcccggaca cggatcggcg cgcgatagcg caccgagagg
     6901 ctgaccgtca ccgcccatat ctcgttgcgg tagagcgacc acgccatcac ctcgtcgagc
     6961 acggtgctga tcacaccgcc gtggaccatc ccggtgtacc cctcgaagcg ttcggcgggg
     7021 acgaatgccg tccacacgcc gcccccctca tcggggtaga acgacaggtg aagaccgtgc
     7081 gcgctgagat cgccacaccc gaagcagttg tgctccatgt cgacattgat gcggccatcg
     7141 tccgcggtcg ccatcctgag cgtgatcctt tcacttcctt tgatgagctc atgcgaacaa
     7201 cccggcgagg tcagggtctc gcatcgctcc cgttgatcac caggtgtgcc agcgcctgca
     7261 acaccggccc cgcctcgcca cgcacgacgg cgttggcaac gtcgtccaac ggcgtcggct
     7321 cgcgattgac gatcaccagc gacgccccgc gcttgcgtgc caccgcgggg agctgcgcgg
     7381 ccggtttgac gaccagcgag ctaccgacca ccagcatcac gtcagccgat gcgctcgcct
     7441 cgatcgcctt gcgcaacgtc tcctcgggca gtgattcgcc gaagaggacc gtggacgagc
     7501 gcagcacgcc gccacaaaca acgcaccggg gatcccgctc gccctgctcc agccgctcga
     7561 cgatctcggc cgtgtcgtag attcggccgc agtcgacaca ccgcagccgg tgcgacgagc
     7621 catgcagctc gagcacccgc gccgggtcgg agccggcttt ttggtggaga ccatcgatgt
     7681 tctgggtgat gaccgcgctg acacgcccgc cgcgctccag cgcggcgatc gcccggtgac
     7741 cggcgttcgg atcgcgcgcc tgcatctcgg gatagcgctt ccggtgggac tgccagcgat
     7801 cctcccggct cgcgcgatct gcgggcacgc tgtccgccgt cgggatgcgg ttggtcttcc
     7861 agacaccgtt gggaccgcga tagtcgggga tgccggattc cgtgctgatg cccgcaccgg
     7921 tgaacacgac catgtgtcga ctcggcgcaa gcagttgagc aaccggctgc aaccgctcga
     7981 gcagcgcggc gtcagcgggc gaatctgtag cagcagaatc gtacgggaac tcatcagtca
     8041 tccgagagcc tcgatccgtt tcgggtgaac ggccgccgcg tctcgccctt ttggcgagac
     8101 ccaatgcttc ccagacgata gcagacgcgc tggagaccct gccgatccca caggccgccc
     8161 acttgtgtac gtacacctgc tgtggtagca tcagcacacg aaaggttgcg ccagctcgtc
     8221 cgaggtcatc ccgctctcga ccgcccgcac cgagtaaagc agcaagccat gttcaacggc
     8281 gatgcggtga agcggcgatc cagcaacggt tccgcccaat acggaactgg tggaaactgc
     8341 gcgccgttcc ggatccatgg gggatccagt acagcgaccc gcaagctgtt cacggcgcaa
     8401 tcggttcgat ggcgacgatt cctggttgct gggtggggag aaagggaatg atgctcatga
     8461 ccgatatggc tgtacgggat atcgaggcgc ggcgggcttc cttgctcggc tcactgcgca
     8521 acctgctgct cgccgtgttt ggacgcgagt cggaccatgg cctgccactc ggcatcaagc
     8581 acaggcagga ccgtaccttt ggcagtgcga tgcgacccgt tgacagaccc acgccgcgcg
     8641 tcgtgcaaga gcagcaatta gccactcccc cgcccagcgg cgagcatcgc tgggagcgac
     8701 atccacagct gcgatctcgt caggcgggcc cggtcccgac cggcagcacg cgatcggtgc
     8761 ggctcgaggc cgagcgcggc ctcatcctgg cgcgcgccgg taagttggtt gaggcggtcg
     8821 aggccttcac cgttgccgcc ggcgaccccg agatcgacct tgccgccctg cccggtttct
     8881 gggatcttcc gcgcaacggc atgctgactg cggtccgggc ctacgaacga gcggaacggc
     8941 tgcgcgatgc cgccactctc gaagcccgta ttcggcacct gctcaaaccg cgctccgtgc
     9001 gtacgacacc agcgacgcgc ccgggccggc tcgctgcctc cggcgactga cgtcgactac
     9061 cagcaccgag cccgcaggtt ccggcgcaga gcgtacctgc tatcaccgaa atgcgatagc
     9121 aggtatggcg agccaacctg gtgtctgcgg atggtgtcac attcggggcc acagtcgtgg
     9181 ttatcggag
//
LOCUS       DASYWL010000217         6621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_310224, whole genome shotgun sequence.
ACCESSION   DASYWL010000217 DASYWL010000000
VERSION     DASYWL010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6621
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310224"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..1796
                     /locus_tag="VGR22_07645"
     CDS             213..1796
                     /locus_tag="VGR22_07645"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEV2128474.1"
                     /translation="MSQSHFMSSVRGHASPHMIRQLVFVGGITSVGTEITASRLVAPY
                     FGTSTFIWANLIGITSTFLAIGYWMSGRLADRFPRPWILYVATAAAALSSILLPYLAQ
                     PILRISLDAFTGVDVGAIYGSLIGVLLLLAIPITLLGFVTPYAVRLLIQNVDEAGDIS
                     GGIYALSTIGSSVGSFLPVLVLIPWIGTARTFLVLGAMLLVPAMVGIWQQHSWGMRVP
                     TMAASMIALLVPFLATPDTIRPAERGHIIYETESADNYIQVIEDDGARYLSLNDGHAF
                     QSIYDPDELLTRGSWDYFMLGPLFTSTESPAELDSAMIIGPAGGTVSKQLTHAFGPVL
                     IDGVEIDKEIVEVGRECFDMTEPNLNVIVEDGRYALRASDKRYEPIAVDAYRQPYIPF
                     QLTSREFFKEVYDHLDDDGTLVVNAGRTAEDYRLVDVIGSTIRSVFPKVYLVDGEAYD
                     NTMVIATRPPSSIETYLQAVENQPEGTILRTVGDRSIETGNIREVPEGRIIYTDDHAP
                     VERLVDRIILDEATGSSTP"
     gene            1793..2629
                     /gene="pgeF"
                     /locus_tag="VGR22_07650"
     CDS             1793..2629
                     /gene="pgeF"
                     /locus_tag="VGR22_07650"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEV2128475.1"
                     /translation="MTSKTADPMTLLRPARSALLDSVAGIAHGVSHRVEGLGEADGNI
                     GMGTPRDKADAWQMRQLWAEAIGVDAETIVTGGQIHEATVLRVTAQDAAKGSRDGIPH
                     LGIGDGLMTDAPNVTLLTLHADCQPILVVDPKRPAIAAVHAGWRGTVKNIAGAVVAAM
                     GSTYDSQPENLLVYLGPALAGCCAEMGPEVTALWRETAARFDIDPEVALSKPGERAHF
                     DAPDANRRLLLAAGVRAEHIDVSPECTRCDTERWFSHRGHGPGAGREGAFIAIRPHRA
                     RA"
     gene            2652..3347
                     /locus_tag="VGR22_07655"
     CDS             2652..3347
                     /locus_tag="VGR22_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEV2128476.1"
                     /translation="MSTITRLEERIHAVREQVAEAARDGGRDPGEVTLIAVSKTFPRE
                     DIEAAYDLGVRHFGENRVQEAQEKVSAPLPDDATLHLIGHLQSNKAKFVPGLFDTVDS
                     VDRSSIIDALDKAVAKADGTLNVLLQVNIAGEEQKAGCAPDEAPALIEQIAATGTLAP
                     RGLMTIAPIDATGDDLHAIFLGLGTLRDDLQAQFPHLDLRELSMGMSNDYREAIAEGA
                     TQVRIGRAIFGER"
     gene            complement(3413..6301)
                     /locus_tag="VGR22_07660"
     CDS             complement(3413..6301)
                     /locus_tag="VGR22_07660"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEV2128477.1"
                     /translation="MACRVFDSQQQGTQTRSFRGRALRPGAGFGLSPSVKRMLVATLV
                     IVGGLVLVFTTASFWINWLWFDSMAYRSVLLRRYAAVALSFLVIGGLAALVFYVNVSL
                     ALRNTRGEETGTAGAISRWSRRILRALIVGASLVILIIAGTVAAQNWEQIMLAVSGRS
                     FGIDDPTFNRDVGFYMFTLPALSGLQSGLVWLVGITTIAVAVIYAVRLGVRFRSWGDV
                     PWAALRHLSGLIAAILLLLAFGYWLRNFDLVFSQRGVVIGPGYTDVNIVRPLNWLMAL
                     SSAAAAILILSGRVLRTPKYLIAVLGGWFLLAVLVTPTLPELVQRTIVNPNEFQREQE
                     YIERNLEMTRAAFGLGDVEVQELTGQESIDPSSLSLDEPPLRNVRIWDYRVVQPIYEQ
                     LQSFVPYYTFDDIDVDRYEIVGVPTQVLVGTRELNVDGLPENAQTWTNRHLAYTHGYA
                     AVVSPVSEVSSDGWPEFLVRQIPPEGPEELAITRPEIYHGEADLDWVIVKTDQGEFSG
                     LVESDDDAVPGFQGDAYGGIALGKPFTRALAALSLGDRNVFLSSQLTGDSELLLWRSV
                     VDRAEHIAPFLAYDPDPYLVIAGGRLVWVIDAYTASDNFPSAKRYDGINYLRNSVKVT
                     VDAYTGDTTFYRTALADPIADAYGRIYPDLFADVSETPPHIAEHFRYPEMQFTMQARV
                     WSEYHVESARSFYDGDDVWSIAEESVGGDLRPMEPFFVTQRLPNEAETVFALTVPFTP
                     AGQQARQNMTAWMAGTAAADGDTDLRLYRFPRQVTVYGPRQIEAQINQSPEISEQITL
                     WNQSGSEVIQGNLLVIPVDNAMLYVQPMYLQASESSAAAPRLARVIVAANNQVVMAPT
                     LGEAIDSLRVPEDNVVEAGDASAVVAEIEADVGPQAPTQPSVSLADLAGMSEDRLLDE
                     AVAAFDRGQEALANGDWSAYGEEQTRLEAILDLLAGEASPPATPEAGP"
     gene            6406..>6621
                     /locus_tag="VGR22_07665"
     CDS             6406..>6621
                     /locus_tag="VGR22_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2128478.1"
                     /translation="MPPPSNDLVIAQSFGSGSSGNALLVRAAGKAILVDCGIGIRKLR
                     AGLANHGMTPSDLDAVLVTHEHGDHIRT"
BASE COUNT         1228 a         2207 c         2086 g         1100 t
ORIGIN      
        1 acaccgcgag gggatagcgg ccagaaggac aacctgtctg cgaccgttct ttgcgtataa
       61 caatgattga gcgcaaaaga ggcgaccaag tccgagcaag gacgatccca tccgtgccgg
      121 gcatccactg accgcatcat atacttggac aagctcgacg ctgagcgtcg cgcgcctgtt
      181 cgacacactg gatgccagga ttaccagcca tcgtgtccca atcgcacttc atgtcgagcg
      241 tgcgcggcca cgccagcccg cacatgatcc gccagttggt cttcgtcggc ggcatcacga
      301 gcgttggcac cgagatcact gcgtcgcggc tggtcgcgcc gtacttcggc acctcgacct
      361 tcatttgggc caacctgatc ggcatcacct cgaccttcct cgcaatcggc tactggatga
      421 gtggccgttt ggccgatcgc tttccccgcc cgtggatact ttatgtcgcg accgccgctg
      481 ccgccctgtc gtcgatcctg ctgccgtatc tcgcgcagcc gatcctgcgt atctcgctcg
      541 acgccttcac cggcgtcgac gtcggtgcca tctacggctc gctgatcggc gtcctgctgc
      601 tgctggcgat tcccatcacg ttgctgggat tcgtgacacc gtacgctgtc cgcctgctga
      661 tccagaacgt cgacgaggct ggcgacatct caggcggcat ctacgcgctc tcgaccatcg
      721 gctcgagcgt tggcagcttc ctgccagtgc tggtactcat cccgtggatc ggcactgcac
      781 ggaccttcct cgtccttggg gcgatgctgc tggttcccgc aatggtcggt atctggcagc
      841 aacacagttg gggcatgcgg gtgcccacca tggccgcctc gatgatcgcg ctactggtgc
      901 cgttcctcgc cacaccggac accatccggc cagcggagcg cgggcacatc atctacgaga
      961 ccgaatcggc cgacaattac atccaggtca tcgaggacga cggcgcacgc tacctctcgc
     1021 tcaatgacgg ccacgcgttt caatcgatct acgacccgga cgagctcctg acacgtggat
     1081 cgtgggatta cttcatgctc ggcccgctct tcaccagcac cgagtctccc gccgaactcg
     1141 acagcgcgat gatcatcggg ccggcaggcg gcacggtcag caagcagcta acccacgcct
     1201 tcggacccgt gctcatcgac ggcgtcgaga tcgacaagga aatcgttgag gtcggccggg
     1261 agtgcttcga catgacagag cccaacctca acgtgatcgt cgaagacggc cggtacgcct
     1321 tgcgcgcgag cgacaagcgg tacgagccga tcgcggtcga tgcctacagg caaccataca
     1381 tcccgttcca gctgacctcg cgggagttct ttaaggaggt gtacgatcat ctcgacgacg
     1441 acggcacgct ggtggtcaat gccggacgca ccgcggagga ctatcgactc gtggatgtca
     1501 tcggttccac gatccgctcg gtctttccca aggtctatct ggtggacggc gaggcctacg
     1561 acaacaccat ggtgatcgcc accaggccgc cgtcctcgat cgagacctac ttgcaggccg
     1621 tcgagaacca accagaaggc actatcctgc gcactgttgg cgatcgcagc atcgagactg
     1681 gcaacattcg cgaggtaccg gaggggagga tcatttatac cgacgaccac gccccggtcg
     1741 agcgattggt agaccggatc atcctcgacg aggcaacagg gagttccact ccatgacgag
     1801 caagacggca gatccgatga ccctcctccg accagcgcgc agcgcgctgc tcgacagcgt
     1861 cgccggcatc gcccacggtg tgtcgcatcg ggtcgagggc ctgggcgagg cggacggaaa
     1921 catcgggatg ggcacaccgc gcgacaaggc cgatgcctgg cagatgcgcc agctctgggc
     1981 ggaggcgatc ggtgttgatg ccgagacgat cgtcaccggc ggtcagatcc acgaggcgac
     2041 ggtcctgcga gtaacagcgc aggatgccgc caagggctcg cgtgacggta tcccgcatct
     2101 cggcatcggc gatggtctca tgaccgacgc gcccaacgtc acactgctga cgctccacgc
     2161 cgactgccag ccgatcctgg tcgtcgatcc gaagcgccct gccatcgcgg cagtgcacgc
     2221 tgggtggcgc gggacggtga agaacatcgc tggtgcggtg gttgccgcca tgggcagcac
     2281 ctacgactcg caaccggaga acctgctcgt gtatctggga ccggccctcg caggctgctg
     2341 cgcggaaatg ggcccggagg tgacggcact gtggcgcgag acggccgcca ggttcgacat
     2401 cgatccggaa gtggcgttga gcaagcccgg tgagcgcgca cacttcgacg cgccagacgc
     2461 caaccgccgc ctgctgcttg ccgccggcgt ccgcgccgag cacatcgatg tctcgcccga
     2521 gtgcaccagg tgcgacaccg agcgctggtt ctcgcaccgt ggccacgggc ccggagccgg
     2581 tcgggagggc gccttcattg ccatccggcc gcaccgagca cgtgcgtgaa ccgaaaggac
     2641 ctgtcgtaga catgagcacg atcactagac ttgaggagcg cattcacgcc gttcgggagc
     2701 aggtcgctga ggcggcccgc gacggcggcc gcgaccccgg cgaggtcacg ctcatcgcgg
     2761 tgagcaagac cttcccgagg gaggacatcg aggcggcata cgatctgggc gttcggcact
     2821 tcggcgagaa ccgggtgcag gaggcgcagg agaaggtttc cgctccgctc ccggacgacg
     2881 ccacgctcca cctgattggg cacctgcagt ccaacaaggc gaagttcgtg ccgggcctgt
     2941 tcgatactgt cgactcagtc gaccggtcct cgatcatcga cgcgctggac aaggcggtgg
     3001 cgaaggccga cggcacgctc aacgtgctgc tgcaggtcaa tatcgccggc gaggaacaga
     3061 aggctggctg tgcaccggac gaggctcccg cgctgatcga gcagatcgcc gcaaccggca
     3121 cacttgcccc gaggggcctt atgacgattg ccccgatcga cgcgacggga gatgacctac
     3181 acgcgatctt tctcggactc ggaacgctcc gggacgacct gcaggcgcag ttcccgcatc
     3241 tcgacctccg tgagctctcg atgggcatga gcaacgacta ccgcgaggcg atcgcagagg
     3301 gcgccaccca ggttcgcatc ggccgcgcta tcttcgggga gcggtagcaa ccggcagcac
     3361 caacggtgag atgggctacc acgcggcgtc caacacccct tgcactccgc agttacggtc
     3421 ccgcttccgg ggtggcaggc gggctcgcct cgccggcaag caggtccagg atcgcctcca
     3481 gccgggtctg ctcctcgccg taggctgacc agtcaccgtt cgccagcgct tcctgtcccc
     3541 ggtcgaacgc cgcgaccgcc tcgtcgagca gccgatcctc cgacatcccc gccaagtcgg
     3601 ccagcgatac cgatggctgc gttggcgcct ggggccccac gtctgcctcg atctcggcga
     3661 cgactgccga cgcatcaccg gcctctacaa cgttgtcctc gggcacgcgc aggctgtcga
     3721 tcgcctcgcc caaggtcggg gccatgacca cctggttgtt tgccgccacg atcacccgcg
     3781 ccagccgcgg tgccgctgct gacgactccg acgcctggag gtacatcggc tggacgtaga
     3841 gcatcgcgtt gtcgaccggg atcaccagca ggttgccctg aatcacctct gacccgctct
     3901 ggttccagag cgtgatctgc tcggagattt ccggagactg gttgatctgt gcctcgatct
     3961 ggcgcgggcc atataccgtc acctgccgcg ggaaccggta gagacgcaga tcggtgtcgc
     4021 cgtcagctgc cgccgttcca gccatccacg cggtcatgtt ctggcgcgcc tgctgcccgg
     4081 cgggcgtgaa cggcaccgtt agcgcaaaga cggtctccgc ctcgttcggc aggcgctggg
     4141 tgacgaagaa cggctccatc ggccgcagat cgccgccgac cgactcctcg gcgatcgacc
     4201 agacgtcgtc accgtcgtag aaggagcgag ccgactccac atggtattcc gaccagacgc
     4261 gcgcctgcat cgtgaactgc atctcggggt agcggaagtg ctcggcgata tggggcggcg
     4321 tctctgagac gtcggcgaag agatccgggt agatgcgccc gtacgcgtcc gcgatgggat
     4381 ccgccaaggc cgtacggtag aaggtcgtat cgcccgtata ggcgtcgacc gtcaccttga
     4441 cgctgttgcg gaggtaattg atgccgtcgt agcgcttggc gctcgggaag ttgtcgctcg
     4501 cggtgtaggc gtcgatgacc cagaccaggc gcccgcccgc gatcacgagg tacggatccg
     4561 ggtcgtaggc cagaaagggt gcgatgtgct ccgcccggtc gaccaccgaa cgccacagca
     4621 gcaactctga atcgccggtg agctgactgc tgaggaagac gttgcggtcg cccagactca
     4681 gcgccgccag cgctcgtgtg aacggcttac cgagagcgat gccgccgtag gcatcacctt
     4741 ggaatcccgg cacggcatcg tcgtcgctct ccacgaggcc gctgaactcg ccctggtccg
     4801 tcttgacgat cacccaatcc aggtccgcct cgccgtgata gatctccggt cgcgtgatcg
     4861 ccagttcctc gggaccctcg ggaggaatct ggcgcacgag gaactccggc cagccgtcgg
     4921 acgacacctc gctcaccggg ctcaccacag ccgcgtagcc gtgagtgtag gcaaggtggc
     4981 ggttggtcca ggtctgggca ttctccggaa gcccgtcgac attcagctcg cgagttccga
     5041 cgagcacctg ggtcggtaca ccaacaatct cgtatcggtc gacgtcgata tcgtcaaagg
     5101 tgtagtacgg aacaaagctc tgcaactgct cgtaaatggg ctgcaccact cggtagtccc
     5161 agatgcggac gttgcggagc ggcggctcgt cgaggctcag gctactgggg tcgatcgact
     5221 cctgccccgt cagttcctgg acctccacgt cgcccagtcc gaaggcggcc cgggtcatct
     5281 cgagattgcg ctcgatgtac tcctgctcgc gctggaattc gttcgggttc acgatcgtac
     5341 gctgcaccag ctccgggagt gtcggtgtca ccagcaccgc cagcaggaac cagccgccga
     5401 gcacggcgat caggtacttc ggggtgcgca gcacgcgccc ggaaaggatc aaaatggcag
     5461 cggcagccga ggagagcgcc atgagccagt tgagcggccg cacgatattg acatcggtgt
     5521 agccagggcc gatcaccacg ccccgctggg agaaaacgag atcgaagttt cgcagccagt
     5581 agccgaaggc aagcagcagc aagatcgcgg cgatgagtcc gctgaggtgc cggagggccg
     5641 cccaggggac atccccccac gatcgaaagc gcacgccgag ccgcacggcg taaatcacgg
     5701 cgaccgcgat cgtggtgatg ccaaccagcc atacgagccc gctctggaga ccgctcagcg
     5761 ccggcagcgt gaacatatag aaaccgacat cgcggttgaa ggtcggatca tcgatgccga
     5821 acgagcggcc gctgaccgcg agcatgatct gctcccagtt ctgcgcggct accgtgcccg
     5881 caatgatcag gatgacgagc gacgcaccga cgatcagggc gcgcaggatg cgtcggctcc
     5941 agcgggagat cgctcccgcc gtgccggtct cctcgccgcg ggtgttccgc agggcgagcg
     6001 agacgttgac gtagaagaca agcgccgcca agccgccgat cacgagaaag gaaagtgcga
     6061 cggcagcgta gcgtcgcagc agcaccgacc ggtatgccat gctgtcgaac cacagccagt
     6121 tgatccagaa tgatgccgtg gtgaagacca gcacgaggcc accgacgatc acgagcgtcg
     6181 cgaccagcat gcgcttcacc gacggggaga ggccgaagcc tgctcccggg cggagtgccc
     6241 tgcccctgaa cgacctcgtc tgcgttccct gctgctgact atcaaacacg cgacatgcca
     6301 tggacgctcc ttcgcatcac gggcgacagc tccggcgccc aagcctcttc atggtacgct
     6361 agctgtcagc gacctgccgg tcatgcgaac ggaaaacccg ctgaaatgcc accaccctcc
     6421 aacgacctcg tgatcgccca aagcttcggc agcgggagtt cgggcaacgc ccttttggtg
     6481 cgcgccgccg gcaaggcgat tctcgtcgac tgcggcatcg gcatccggaa gctccgggcg
     6541 ggcctcgcta accacggcat gacgcccagc gacttggatg cggtgctggt cacgcatgag
     6601 cacggagacc atatccggac g
//
LOCUS       DASYWL010000218         7167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_104283, whole genome shotgun sequence.
ACCESSION   DASYWL010000218 DASYWL010000000
VERSION     DASYWL010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7167
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104283"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2202
                     /gene="polA"
                     /locus_tag="VGR22_07670"
     CDS             <1..2202
                     /gene="polA"
                     /locus_tag="VGR22_07670"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEV2128479.1"
                     /translation="YKALVGDTSDNIPGVPGIGDKTAKKLIAEFGDLEQITAHIDEIS
                     PTRARNALRENIEQARKSKELATIVCDIELNLALDEAQVGHFDLTEMTELFRELEFRS
                     LLNRLPQSTMEPSESTTVQAGADRQTQRVTVTTETELDDLVEALQHAAAIALDVETDS
                     TDPISANLVGISVATDTAHSWYIPVGHRDGNQLSLDPVRGKLDPALSREGLRLVAHHG
                     KFDLHVLQRHGFTLDRVDFDTMIAAFLIGDSSIRLKDLAFTRLGIQMTEISELIGTGR
                     NQDTMDNVAVELAAPYACADVECTMGLVDPLRKDLEERALLPLFEEIELPLVPVLVGM
                     EKRGIAIDTDELKMFSEEITRRLQEVEAEIDALAGRPVRVGSNKQMATVLFEELRLPP
                     GRKTKTGYSVDSSVLETLRDQHPIIEWILEYRTLSKLKSTYVDSLPNEVNTATGRIHT
                     NFNQTVAATGRLSSTGPNLQNIPIRTEVGRRVRRAFVADHRPEHRIVENAILLAADYS
                     QMELRILAHMSGEPFLIEAFREGEDIHRATAALVHQIDFGDVTADQRRVAKTVNFGIL
                     YGMQAFGLSRDTGMAREDATRFIEAYWERLPRVRQFFDEILAFGQEHAYVQTLSGRRR
                     YIPELNSRNGQQRQAAQRVAMNMPIQGTQADIIKRAMIDLDRALQDQELPGYQVLQVH
                     DELVLEIDETRLHDIAVLVRDTMQNAQQLEVPVIADLRAGMNWEDMHGHEP"
     gene            2315..2629
                     /locus_tag="VGR22_07675"
     CDS             2315..2629
                     /locus_tag="VGR22_07675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEV2128480.1"
                     /translation="MPTYVYRCDSCKHQFEIFQKMSDDALDTCPRCEARVRRIIHPVG
                     IVFKGSGWYITDSKSSGSSGDTAAASSDADGSGADKKPSDTDSKSKSKSSDKETAKTA
                     AD"
     gene            2875..4323
                     /gene="cysS"
                     /locus_tag="VGR22_07680"
     CDS             2875..4323
                     /gene="cysS"
                     /locus_tag="VGR22_07680"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEV2128481.1"
                     /translation="MQTTPETHTSAPSTSPPLMIYNTMTRRKEPFETIEPGKVRMYVC
                     GVTVYDDAHIGHGMSYIRFDMIRRYLEHLGYDVLHAQNFTDIDDKIINRAAAEGLDVD
                     ILVEDLIRDWLAEVQALGLLPAKVYPRATQEVPEIIALIEGLIQRGHAYEAGGDVYFR
                     VRSFADYGKLSGRDIEELRSGARIEIDERKEDPLDFALWKAAKPGEPKWDSPWGEGRP
                     GWHIECSAMCMDHLGGMVDIHGGGRDLIFPHHENEIAQSEAFLGEEPFARYWVHNGML
                     QLDGEKMSKSLGNVVKLRSLIERGRAVAFRLMVLQSHYRAPLNYTEEGLEAAHSGLQR
                     LRAAAGLAASDGEARGELADLAESSHERFHAAILDDFDTPTAMAALFELARTINREAA
                     AGGAPGLASARDTLIELAGTLGIDLLREEAATASSSDVEALMQILIDVRAELRKKKQF
                     AIADMLRDRLQEKGFILEDTRTGTTWKREATA"
     gene            4274..5167
                     /gene="rlmB"
                     /locus_tag="VGR22_07685"
     CDS             4274..5167
                     /gene="rlmB"
                     /locus_tag="VGR22_07685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEV2128482.1"
                     /translation="MRTPEQGRRGSGKRRPDRGTKRPGGPAGRGPGANRPNRPQQGQR
                     RQGSGREQLTVADPRLLFGRNAVVESLRGRRPPPKRVWIAEGIKRDERVEEIRQLALE
                     AGVPVEEAPRMLLDDMTTHANHQGVVLDPADYPYADIEDLIETGQTILILDHIQDPQN
                     FGSLIRAGDATGIGGVILPQDRSVAVTPAVVNASAGAVEHVPVALVANLNRAAEKLID
                     SGYWVAGLAGDEESAIDLFEADLPTPLALVVGSEGRGISEAVRSKCQLLLRLPMTGKV
                     ASLNAATAGTVALYEVVRQQG"
     gene            5291..>7167
                     /locus_tag="VGR22_07690"
     CDS             5291..>7167
                     /locus_tag="VGR22_07690"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEV2128483.1"
                     /translation="MAHETVDLAASFASRISPQFSRRILVKAAAGFAAAAAMARGGVP
                     QAQPTIAQGETTRASEWWQPAGASSDGGYIEFTADIVFRAIAPHWAGERGVHGAVEIS
                     VSTDGATYTDPQIIGPAHSDAGPPDRDGRTFGQLMMTGPARYVRYRTLDANGDPIALP
                     SLAFTYIDASNGPTIDDVQPETFGGALTKPPVITREQWGAALAYGGWDGGTHEWTPQY
                     RAVEHIIIHHSVTPQFRDPLVEIRSIHYYHAVTRGWGDIGYNYLVDFLGNVYEGRKGG
                     DNVVGGHAFQYAYGSSGICSMGSFELESSTPEAIAALVWITAWVGRDLDPLGRSDFHE
                     TPNCPTIAGHRDVNDSTCPGDGLYADLPYIRTAVAEILAGSRNAGVPSGYDPGDVVQT
                     TVSSANLRSKPSTSASIEARLDRGKVLTVIEGPTTNDGYTWYRVSGEKSAGWLATVTF
                     ARSSAPPPQRDYEPNARVEIAADLLNLRSEPGLWASVVARLPQGDTATVVDGLSTSGG
                     LTWLKLDTNLGTGWVAQQFLAPSGRALIASKFAIGDTVRVTTNPLNIRTDTSLQSRII
                     AKLWEGETGTVIGGPRRSAGISWVQIRTDRGSGWVSEEYLEEGSAPPPRTVNGKFSPG
                     D"
BASE COUNT         1384 a         2427 c         2241 g         1115 t
ORIGIN      
        1 tacaaggcgc tcgtgggcga cacctcggac aacatcccgg gcgtgccggg catcggcgac
       61 aagacggcga aaaagttgat cgcggagttc ggcgatctcg agcagatcac cgcccatatc
      121 gacgagatca gcccaacccg cgcccgcaat gcgctccgcg agaacatcga gcaagcccgc
      181 aagagcaagg agcttgcaac catcgtctgc gacatcgagc tgaatctggc gctcgacgag
      241 gcccaggtcg gccacttcga tctcaccgag atgaccgagc tgttccggga actcgagttc
      301 aggtcgctgt tgaaccgact gccgcaatca accatggagc cgagtgagtc gaccactgtc
      361 caggccggcg cggaccggca aacccagcga gtgacggtca cgaccgagac cgagcttgac
      421 gacttggtcg aagcgctgca acacgccgcc gcaatcgcgc tcgacgtcga gaccgactca
      481 acggacccga tctccgccaa cctggtcggc atctccgtcg cgaccgacac cgctcactca
      541 tggtatatcc ctgtcggcca ccgcgatgga aaccagctct cgctcgaccc ggtccggggg
      601 aagctcgacc cggcgctcag ccgcgaggga ctccggctcg tcgcgcacca cggcaagttc
      661 gacctccacg tgctgcaacg tcacggtttc accctcgatc gggtcgactt cgacacgatg
      721 atcgccgcct tcctcatcgg cgactcctcg atccggctca aggacctcgc cttcacccgg
      781 ctcggcatcc agatgaccga aatctccgag ctgatcggga cggggcgcaa ccaggacacg
      841 atggacaatg tcgccgttga gctggccgcc ccctatgcgt gtgccgatgt cgagtgcact
      901 atgggactgg tcgacccgct gcgaaaggat cttgaggagc gcgctctgct gccgctcttc
      961 gaagagatcg agctgccact ggtgcccgtg ttggtcggca tggaaaagcg cggtatcgcc
     1021 atcgacaccg acgaactcaa gatgttctcg gaggagatca ccaggcggct gcaggaggtt
     1081 gaggcggaga tcgacgcctt ggccggccgg ccggtccgcg tcggatccaa caagcagatg
     1141 gcgacggtgc tcttcgagga gctgaggctg ccaccgggcc gcaagaccaa aaccggctac
     1201 tccgtggatt ccagcgtgct cgagaccctc agggatcagc acccgatcat cgagtggatc
     1261 ctcgagtacc gcaccctttc gaagctcaaa tccacctacg tcgactcgct gcccaatgag
     1321 gtcaacacgg ccacgggtcg cattcacacg aacttcaacc agacggtcgc ggcgacaggt
     1381 cgcctctcct ccacggggcc gaacttgcag aacatcccga tccgaacaga ggtcgggcga
     1441 cgcgtccgcc gggcgttcgt cgccgaccac cggcccgagc accgtatcgt cgagaacgcg
     1501 atcctgctcg ccgccgacta ctcccaaatg gagctgcgca tcctcgccca catgtccgga
     1561 gagccgttcc tcattgaggc cttccgggag ggcgaggaca ttcaccgagc gacggccgcc
     1621 ctcgtccacc agatcgactt cggggacgtc accgcggatc agcgccgggt cgccaagact
     1681 gtcaatttcg gcattctcta cggcatgcag gcattcgggc tctcccgcga caccggcatg
     1741 gcacgcgaag atgccacccg gttcatcgag gcgtactggg aacgattgcc gcgcgtccgt
     1801 cagttcttcg acgagatcct cgcgttcggg caggaacacg cctacgtcca gaccctctcc
     1861 gggcgtcggc gctacattcc tgagctcaac agtcgcaacg gccagcagcg gcaggcggcg
     1921 cagcgcgtgg cgatgaacat gccgatccag ggcacgcagg ccgacatcat caagcgcgcg
     1981 atgatcgatc tcgacagggc actgcaggac caggagctgc ccggctacca ggtcctgcag
     2041 gtgcatgacg agcttgtcct cgagatcgac gagacccgcc tgcacgacat cgccgtgctg
     2101 gtgcgcgaca cgatgcaaaa cgcacagcag ctggaggtac cggtgatcgc cgacctccga
     2161 gcaggcatga attgggagga catgcacggc cacgaaccgt agcgtccccg cccggccgcg
     2221 gacagactcg ttgatttcat cgtagaatac atggtgcgtc ggaccattgt atggtccatg
     2281 aacggctgtg caatgtttca ggagaaccag aggaatgccg acctacgtct accgttgcga
     2341 ctcatgtaaa catcagttcg aaattttcca gaagatgtcc gacgacgcgc tggatacctg
     2401 cccgcgatgc gaggccaggg tccgaaggat catccaccca gtcggcatcg tcttcaaggg
     2461 cagtggctgg tacatcacgg acagcaagtc gtccgggagc agcggtgaca ccgctgcggc
     2521 ttcaagcgat gccgacgggt ctggcgcaga caagaagccg tcggacacgg acagcaagag
     2581 caagagcaag agcagcgaca aggaaactgc caagacggcg gcggactgac aatcaccacc
     2641 acgcacccct ctcgcccgga gtagccaccc aatgaccttc cacgcggcta cacgacatca
     2701 acgcatcgtc gacgcgagcg ccaccgtgac ggcgcaactc gctccgggct gcgtctgtcc
     2761 cctggagtag gctacacggc accaacgtgg cctgccccaa ccccatcgag gctcccgttg
     2821 cccggtcggt ttccatcgac cgggccgcca gtcgctgaac gagaaggaca gaccatgcag
     2881 accacacccg aaacacacac ttccgcaccg tcgacgtctc caccgctgat gatctacaac
     2941 acgatgacgc gccggaaaga acccttcgaa acgatcgagc ccggcaaggt gcgcatgtac
     3001 gtctgcggtg tgaccgtcta cgacgacgcc catatcggcc acggcatgtc gtatatccgc
     3061 ttcgacatga tccggcgcta ccttgagcac ctcggatacg acgtcctgca cgcacagaac
     3121 ttcaccgaca tcgacgacaa gatcatcaat cgcgccgctg ccgaggggct cgacgtcgat
     3181 atcttggtcg aggacctcat ccgcgactgg ctcgcggagg tccaggcgct cggtctcctg
     3241 cccgccaagg tctacccgcg cgcgacgcag gaggtgccgg agatcatcgc gctgatcgag
     3301 gggctgatcc agcgcggcca cgcctacgag gccggcggcg acgtctactt ccgcgtccgc
     3361 tcgttcgccg actacggcaa gctctccgga cgcgacattg aggagctgcg ctccggcgcc
     3421 cggatcgaga tcgacgagcg caaggaggac ccgctcgact tcgcgctgtg gaaggccgcc
     3481 aagcccggcg agccgaaatg ggacagcccg tggggcgagg ggcgacctgg ctggcacatc
     3541 gagtgctccg ccatgtgcat ggaccacctg ggcggcatgg tcgacatcca cggcggcggc
     3601 cgcgatctga tcttcccgca tcatgagaac gagatcgccc aatccgaggc gttcctcggc
     3661 gaggagccgt tcgcccgcta ctgggtccac aacggcatgc tccagctcga cggcgagaag
     3721 atgtccaagt cgctcggcaa cgtggtcaag ctccggtcgc tgatcgagcg cggccgtgcc
     3781 gttgccttcc ggctgatggt gttgcagtcg cactaccgcg cgccgctcaa ttacaccgag
     3841 gaggggctgg aggcggcgca cagtggcctt cagcggcttc gggcggccgc tggcctagca
     3901 gccagtgacg gcgaggcccg cggcgagctt gcggatttgg ccgagagcag tcacgagcga
     3961 ttccacgccg ccatactgga cgacttcgac acgcctacgg caatggcggc gctcttcgag
     4021 ctcgcacgca ccattaaccg agaggcggcc gcgggcggcg cgccgggtct cgcctccgcc
     4081 agggacacgc tgatcgagct ggccggcacc ctcgggatcg accttctgcg cgaggaagca
     4141 gcaacggcca gtagctcgga cgtcgaggcg ctgatgcaga tcctgatcga cgtccgagcg
     4201 gagcttcgca agaagaagca gttcgccatc gccgacatgc tgcgcgatcg gctccaggaa
     4261 aagggattca tccttgagga caccagaaca gggacgacgt ggaagcggga agcgacggcc
     4321 tgaccgcggc acaaagcggc ccggcggtcc ggccggacga ggccccggcg ccaaccgacc
     4381 gaaccgtccg cagcagggtc agcgccgcca gggaagtggt cgcgagcaac tgacggtagc
     4441 cgatcctcgc ctgctcttcg ggcgcaacgc cgtcgtggag tcgctccgcg gccgtcggcc
     4501 cccaccgaag cgtgtttgga tcgccgaggg catcaagcgc gacgagcgcg tcgaggagat
     4561 ccgccagctc gcgctggagg cgggcgtgcc cgtcgaggag gcgccgcgca tgcttctcga
     4621 cgacatgaca acgcatgcca atcaccaggg ggtggtgctc gacccggccg actaccccta
     4681 cgccgacatc gaagacctga tcgagacggg acagacgatc ctgatcctcg accacatcca
     4741 ggacccgcaa aacttcggct cgctgatccg ggccggagat gcaaccggca tcggcggcgt
     4801 gatcctgccg caggaccgat cagtcgcggt gacgccagcg gtggtcaatg ccagtgccgg
     4861 cgctgtcgag cacgtaccgg tcgcactcgt cgccaacctg aatcgtgcag cggagaaact
     4921 gatcgactca ggctactggg tggccgggct cgccggcgac gaggagagcg ccatcgacct
     4981 gttcgaggcc gacctgccca ccccacttgc gctggtggtc ggctccgagg gccggggcat
     5041 ctcggaggcg gtgcgcagca agtgccagct cctgctgcgc ctgccgatga ccggcaaggt
     5101 cgcctcgctc aatgcggcga ccgcaggaac ggtcgcgctc tacgaggtcg tccgccagca
     5161 gggctgagca tcaggtgtgt tgacagtttg gcacatctct gttacggtgt acgtacattg
     5221 tgtacgggcg ccataacagg gttcactggt tgctggcgtc cgatcttccg gtggggggaa
     5281 ggacgcacct ttggcgcacg agacagttga ccttgcagcc tcattcgctt cgcgcataag
     5341 cccgcagttc agccggcgca tactcgtcaa ggctgccgcg ggctttgctg ccgccgcagc
     5401 catggcgcgc ggaggtgtgc cccaggcaca gcccaccatc gctcagggtg agaccacccg
     5461 ggccagcgag tggtggcagc cagcgggagc tagcagcgac ggtgggtata tcgagttcac
     5521 ggcggacatt gtctttcgcg ccatcgcgcc gcactgggca ggtgaacggg gcgtacacgg
     5581 tgccgttgag atctcggtga gcaccgacgg tgctacctac accgatccgc agattatcgg
     5641 tccggcgcat tcggacgcag gaccgcccga ccgcgacggg cgcacgttcg ggcagctcat
     5701 gatgacgggt cccgcccggt atgttcgata ccgcacactg gatgcgaacg gcgacccgat
     5761 cgcgctgccc agtctcgcct tcacgtacat cgacgcctcg aacggcccca cgattgatga
     5821 cgtgcagccg gagacgttcg gcggcgcgct caccaaaccc ccggtcatca cacgtgagca
     5881 gtggggtgcg gcgctggcct acggcggctg ggacggcggc acacacgagt ggacgccgca
     5941 gtaccgggcc gtcgaacaca tcatcattca ccactccgtg acgccgcagt tccgcgaccc
     6001 gctcgtcgag atccggtcga tccactacta ccacgccgtc acacgtggct ggggcgatat
     6061 cggctacaac tacctcgtcg atttcctcgg caacgtctac gagggacgca aaggcggcga
     6121 caacgtcgtc ggcggccatg cgtttcagta cgcctatggc agctccggta tctgctcgat
     6181 gggctccttc gaactggagt cgtcgacgcc agaggcgatc gccgcactcg tgtggatcac
     6241 ggcctgggtc ggtcgcgatc tcgatccgct gggcaggagc gattttcacg agacgcccaa
     6301 ctgcccgacc atcgccggcc accgcgacgt caacgacagc acctgcccgg gcgacggcct
     6361 ctacgccgac ctcccctaca tccgcacggc tgtcgccgag attctcgccg gcagtcgcaa
     6421 tgccggcgtt ccctcgggct acgaccccgg cgacgtcgtc cagacgacgg tcagcagcgc
     6481 caacctgcgc agcaagccca gcacgagcgc ctcgatcgaa gcccgtctcg atcgtggcaa
     6541 ggtgctgacg gtcatcgagg gaccgacgac caacgacggg tacacctggt atcgggtctc
     6601 tggcgagaaa tcggccggct ggctcgccac ggtcaccttc gctcgctcct ccgctccgcc
     6661 gccacagcgc gactacgagc cgaacgcgcg ggtggaaatc gcagccgacc tgttgaacct
     6721 ccgctcggag ccggggttgt gggcttcagt cgtcgcacgc ctgccacagg gcgatacagc
     6781 caccgtggtc gacggtctct caacgagcgg cgggctcacc tggctgaagc tcgacaccaa
     6841 cctcggcacc ggctgggtcg cccagcagtt cctcgcacca tccggtcgcg ctctgatcgc
     6901 ctcgaagttc gccataggcg acacggtcag agtcacgaca aatccgctca acatacgcac
     6961 cgataccagc ctgcaatccc ggatcatcgc caagctctgg gagggagaga ccggcaccgt
     7021 tatcggaggc ccgcgtcgct cggcgggaat cagctgggtc cagatccgca ccgatcgtgg
     7081 atccggctgg gtatctgagg agtatcttga ggaaggcagc gcgccgccgc caagaacggt
     7141 caacggcaag ttttcccccg gcgatga
//
LOCUS       DASYWL010000219         9662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_198100, whole genome shotgun sequence.
ACCESSION   DASYWL010000219 DASYWL010000000
VERSION     DASYWL010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9662
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198100"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VGR22_07695"
     CDS             <1..277
                     /locus_tag="VGR22_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128484.1"
                     /translation="LARESDWSVIPSTFAAERARVHGVLRAHAHWVEPLSTETGPHID
                     AMRERIGRARELDPTAGGFAGIFATGPAGLEATEAARRRFLGEDLVS"
     gene            499..1539
                     /locus_tag="VGR22_07700"
     CDS             499..1539
                     /locus_tag="VGR22_07700"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEV2128485.1"
                     /translation="MHRRLIRGLLVLMGLALLVPLAVMPATAAESVAAWVETATKTPG
                     VGCGVDVSVEVRASGTAVSSADVSVTLVQDGTSNVISIDRSTTNSRGIAWLTMDTSAG
                     WDGLKGWMEVAVNGSYIGGQTIWVTDGACSGNSTLVEMSGKATTTIRAGSASTASSSS
                     AESSGGRVTVPNVWTYQQERSLSCEYAALSIATGALGTWVNEYRFESVVPQSVNPHWG
                     YRGNINGAWGNTDDYGVYASALVPALQHFGFNGTVFYGGKADLMAAIDRGEPALVWIG
                     LGGDLSHNEYTADGTRFQLTRYMHVMVAYGYDSWGVYLSDPGTGSYKSYSWGDFLWMW
                     DIMDGMALSVSR"
     gene            1689..2876
                     /locus_tag="VGR22_07705"
     CDS             1689..2876
                     /locus_tag="VGR22_07705"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II histone deacetylase"
                     /protein_id="HEV2128486.1"
                     /translation="MEHPRDRKVGLGFDDRYLQHNSGEFLIEYRDRYPYAEPVPHLSS
                     PAVLGRAKHLIDLYGLSRLMEPIGTREATDEELALYHTESHIQRVREIGKTGGDTGTG
                     APIGPRGDVIARLAVGGVIEGVAAVMRGDVHHALCLVRPPGHHAMSDHGMGFCTFNNV
                     AIGAKVARQLGARRITIFDWDVHHGNGTQDAFWEDPDVLFISIHQEDLFPPVGWGSID
                     QRGEGAGEGTTINIPLPAGSGNPSYLAAMEETVVPAIRAFGPDLIMVSAGQDASVMDP
                     LGRMSLTTASYRVMTSMLRELADDICESRMVIALEGGYSEIYAPFCTAAIAEGLVTGI
                     GGFDPVREPYGSRAETMPATRRVGKDARTALDAAQEAHADLLADAPIEPSTVDDSGST
                     VPR"
     gene            2900..3541
                     /locus_tag="VGR22_07710"
     CDS             2900..3541
                     /locus_tag="VGR22_07710"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV2128487.1"
                     /translation="MPLTVIFVRHGESQANVDGIFANRPDYPAPLTPAGIVQARTLAH
                     VLASERVTHLYTSPLFRAVETAEVLASKLDVEIEIRDALREYDVGEFEGLPYADEHAW
                     RWDAYERVDRSWQRGDRQACLPGGESLADIDARFLPFMESLAARHAASDRLVIVSHGG
                     LYRVALPGLCRTLTAHDTRRLPMDHCTSIIVVHEDGDWRCLTWDGKHPPFACP"
     gene            3608..3832
                     /locus_tag="VGR22_07715"
     CDS             3608..3832
                     /locus_tag="VGR22_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128488.1"
                     /translation="MAAEAEGGDAESGTRKVIYAALFANLLIAIAKFIVGAIQYELRV
                     IDPWGNTFEGRLGHRSARCGRSTATERTTP"
     gene            3887..4147
                     /locus_tag="VGR22_07720"
     CDS             3887..4147
                     /locus_tag="VGR22_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128489.1"
                     /translation="MPLFEDAALTGVLIAAIGLGIVQITDNRVYDGVASIGIGIVLIY
                     VAIQLGAEFRALLIGEAVSPNDRERMREAMESPPRCRPRCAC"
     gene            4123..4350
                     /locus_tag="VGR22_07725"
     CDS             4123..4350
                     /locus_tag="VGR22_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128490.1"
                     /translation="MSTALRLLTMHLGPHDVLVNAEIHLRNELDTDRIERLLADITRR
                     LREENPKVSQTFIELHAPDQKPAAWQTTGGG"
     gene            complement(4369..5157)
                     /locus_tag="VGR22_07730"
     CDS             complement(4369..5157)
                     /locus_tag="VGR22_07730"
                     /inference="COORDINATES: protein motif:HMM:NF024819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside adenylyltransferase
                     domain-containing protein"
                     /protein_id="HEV2128491.1"
                     /translation="MDPDSISELTPHRDINAILVELTSRIGEITGEVLTGCYLTGSLT
                     YGGFERGSSDIDYLAIMRQPMPASVRAVLEAAHAVIAQRFPEWAERIEGSYITEAMLG
                     SLDPPPEPRPYINGGMFWDPDPRYGNEWLINLYALRSCGVALIGPDPERVIPPIAIED
                     VREASRRDLLEEWVPKLEDRAFFASSHQQAYVTLTICRVLHRAENDEVVSKRVAARWV
                     RERYPDPWIIDLVDRAERWEHGQRMGDPQAARNFILYARNCLGE"
     gene            5318..5851
                     /locus_tag="VGR22_07735"
     CDS             5318..5851
                     /locus_tag="VGR22_07735"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEV2128492.1"
                     /translation="MDWLLDIDWRGMFIPDTAIVELVIRGSIMYLALYFLLRVVLKRQ
                     SGGIGITDLLVIVLIADAAQNAMGDDYQSIPNGIILVATIIFWSYTLDWLGYRFPRIE
                     PLVYAKPLQLVREGQMLRHNMERETMTEGELMSQLRLQGVEDISVVKVAYLEGDGRVS
                     VIVDGSKDKGASPRHRP"
     gene            complement(5881..6951)
                     /locus_tag="VGR22_07740"
     CDS             complement(5881..6951)
                     /locus_tag="VGR22_07740"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEV2128493.1"
                     /translation="MSDARYPYESLLAFAIDAFVTAGLTQDQAAEAGGLLLQSDLRGI
                     ASHGLGRLAGYVSRLQRGFDRADVEVEVMRESPVTLALDGHLGLGLLVGPEAMRRTIA
                     KAETNGICMTTVRNSNHFGIAGNYALMAATQGLGGLAMTNSSRVVVPLFGRTTRLGTN
                     PIAFAVPTSAEPFVLDMSTSTVAWGKIEIARRAGLPIPEGWGVDDEGRTTTDPHQVVG
                     LTPLGGIRERSGHKGYGLGLMVEILTGPLSGGVWSNHVNRQFEQTFPPGTGHFFMAWR
                     IDAFRDPQEFIVAMDQMVCELRETPAADDAPGPVLVPGDPEVAAERDNRENGIPVAPG
                     LARELTEMARRIGIDHPFQVAK"
     gene            7349..7591
                     /locus_tag="VGR22_07745"
     CDS             7349..7591
                     /locus_tag="VGR22_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128494.1"
                     /translation="MQTNRKAVRFYIDRDADTPVVRSVPVSHPSVAAVSASRAPITHR
                     PENGDESAMAFAAAESRRRGARHLLTALRKPRRNAS"
     gene            7635..9047
                     /gene="mgtE"
                     /locus_tag="VGR22_07750"
     CDS             7635..9047
                     /gene="mgtE"
                     /locus_tag="VGR22_07750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEV2128495.1"
                     /translation="MTETRQPPAQEPITDQEDEDAPLIEQIRVAVAERDVERARGLAR
                     RMVEDDALALLEDLSPDELSRLFAFLGDESLALLLSRLDDRDAAGILTRMTAAQAADI
                     LEEIDPDDATDIFAEMEQSDPAAAGTILIEMEPDEAAEIRDLMAYPPDTAGGIMTPAF
                     VAVFPDLRADETVRALRQVAEEAETVNYVYVISREDESLLGVLPLHRLVLSRPNAPVG
                     DLMTPEILSVPVHADQEEAARLVTEHDLLAIPVVDDDNRLLGIITSDDVADILEEEAT
                     EDIERLGGSQPLAVPYLRASPFLLFRRRIIWLLVLFMAQFFTVTIQENYADVLEEVIL
                     LSAFIPILIGTGGNVGSQTVSTIIRAMALGEVQPHHILHVVGKEAITGVALGLVMGLL
                     MFFRAELSAGSSVQIGLTVGATVLVLTIWAATVGAIIPIVLSKLKMDPAVVSAPFISS
                     FVDGTGLIIYFTLARAFLDL"
     gene            9220..>9662
                     /locus_tag="VGR22_07755"
     CDS             9220..>9662
                     /locus_tag="VGR22_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128496.1"
                     /translation="MQPSVVAVITEETQNDIVAALHAARVGHLTRVIRRERGAIAAQL
                     GRAGIDTAELPAAIDAADRTVLISPASHALETACLVLQRGAVSAWTLTNDDGWQPIDD
                     KAIEITATRGFPARPVAPAHRSGRTFRKPNVHRRPRRDIVTPPTSA"
BASE COUNT         1695 a         3237 c         3069 g         1661 t
ORIGIN      
        1 gctcgcgcgc gagtcggact ggtcggtcat cccgtcgacg ttcgcggccg agcgagcgcg
       61 tgttcacgga gtgcttcgcg cccacgccca ctgggtggag ccgctatcga ccgagaccgg
      121 tccgcacatc gatgccatgc gcgagcggat cggccgcgct cgcgagctcg atcccaccgc
      181 gggcggattc gccggaatct tcgccaccgg tcctgctggc ctggaggcca ccgaggccgc
      241 acgccggcgc ttcctcggcg aagacctcgt atcctgacct tgcgcgtgta cgtacacctg
      301 tgcagaatag acagcatata tgtgcctcgg tgtcaccatc gaggttggtc ccaaggatgg
      361 ctgcaatcgc cgtgggtggg tgctacagtt gcgtgaaacg caggccgcgt tcacgacggc
      421 tagtgactca agtcagcccg catggggatc cacccgggtc cgtcgggaac aggtgaagga
      481 acgctggggg gagtttcgat gcacagacga ttgatccggg gtctcctggt tctgatgggc
      541 ttggccctgc tggtgcctct tgctgtgatg cctgccacgg cagcggagag tgtggcggcg
      601 tgggtcgaga ccgcaacgaa gacgccgggt gtcggctgcg gtgtcgacgt cagtgtcgag
      661 gtgcgcgcca gtggcacggc ggtctccagc gccgacgtct ccgtcacact cgtgcaggat
      721 ggcaccagca acgtcatctc catcgaccga agcacgacca acagccgcgg catcgcctgg
      781 ctgacgatgg acaccagcgc cggctgggac ggcctcaagg gatggatgga ggtcgccgtc
      841 aacggcagct atatcggtgg acagacgatc tgggtgacag acggcgcctg ctccggcaac
      901 agcaccctcg tcgaaatgag cggcaaggcg accaccacga ttcgtgccgg atctgcaagc
      961 acagcgtcat cctcgtcggc tgagagctcc ggtggcaggg tgacggtgcc gaacgtatgg
     1021 acctaccagc aggaacgcag cctgagctgc gagtacgccg cgctttcgat tgcaaccggc
     1081 gcccttggca cgtgggtgaa tgagtaccgg ttcgagtccg tcgtgccgca gagcgtcaac
     1141 ccgcactggg gctatcgcgg caacatcaac ggcgcgtggg gtaacaccga cgactatggc
     1201 gtctacgcct cggcgctggt gccggccctg cagcacttcg gcttcaacgg aaccgtcttc
     1261 tacggcggga aggcagatct catggccgcc atcgaccgtg gcgagccggc tctcgtctgg
     1321 atcggactgg gtggcgacct gagccacaac gagtacacgg cggacggcac gcgcttccag
     1381 ctgacgcggt acatgcacgt gatggtggcc tacggctacg actcctgggg tgtttacctc
     1441 tccgatcccg gcacgggaag ctacaagtcc tactcctggg gtgacttcct ctggatgtgg
     1501 gacatcatgg acggcatggc actcagtgtc tcgcggtaac gcggtaacct gccatcgaca
     1561 acaaccgaac gtttcaaatg cgggctggca tactgccagc ccggtgttgt gttgcgcgtt
     1621 gccgagccgc cccatatagt ccgattcagt tagccgccga acgttcggcc ttgggagagc
     1681 acgacacaat ggagcaccca cgcgaccgca aggtcggact tggattcgat gaccgctatc
     1741 tccagcacaa ctccggcgag ttcctgatcg agtaccgcga tcgctacccc tacgccgagc
     1801 cggtaccaca tctctccagc ccggccgtcc tcggccgcgc caagcacctg atcgacctct
     1861 acggcctctc ccgcctgatg gaaccgatcg ggacccgcga ggcaacggac gaggagcttg
     1921 cgctctacca caccgaatcg cacatccagc gcgtgcgcga gattggcaag accggaggtg
     1981 acacgggtac cggcgcgccg atcggtccgc gtggcgatgt cattgcccgc ctggccgtcg
     2041 gcggcgtaat cgaaggagtt gctgcagtga tgcgcggcga tgtccaccac gcgctctgcc
     2101 tcgtccgccc ccccggacac catgcgatga gtgaccacgg catgggcttc tgcaccttca
     2161 acaacgtcgc gatcggtgcc aaggtcgcca ggcagctcgg cgcgcgccgg atcacgatct
     2221 tcgattggga tgtccaccac ggcaacggca cacaggacgc cttctgggaa gaccccgacg
     2281 tgctcttcat ctccatccac caggaggatc tcttccctcc cgtcggctgg gggtcgatcg
     2341 accaacgcgg cgagggtgcc ggcgagggaa cgacgatcaa catcccgctg cccgctggca
     2401 gcggcaaccc gtcctacctc gcggcaatgg aggagaccgt ggtgccggcg atccgcgcgt
     2461 tcgggccgga cttgatcatg gtctccgccg ggcaagacgc ctcggtgatg gacccgctcg
     2521 gccgaatgtc gctcaccacc gcaagctatc gcgtgatgac gagcatgttg cgcgagctgg
     2581 ccgatgacat ctgcgagagc cgaatggtga tcgcgctcga gggtggctac agcgagattt
     2641 acgcaccgtt ctgcacagcg gcgatcgccg aggggctggt taccggaatc ggcgggttcg
     2701 atccggtacg ggagccgtac gggtcccggg cggaaaccat gccggccact cgccgcgtcg
     2761 gcaaggatgc ccggaccgcg ctcgacgcgg cccaggaggc ccacgccgat ctgctggcgg
     2821 acgcgccgat cgagccgtcc accgtcgacg actcgggatc gactgtgccg cgctagctgg
     2881 cacacctccg agcgcgtcga tgccactcac cgtcatcttc gtccgacatg gtgagagcca
     2941 ggcgaatgtc gatggcatct tcgccaaccg gccagactat ccagcgcccc tcaccccggc
     3001 cggaattgtg caggcaagga cgctcgcaca cgtcctcgcc agcgagcgcg tcacacacct
     3061 ctacacgagc ccgctgtttc gcgctgtcga gacagcggag gtgctggcct cgaagctcga
     3121 cgtcgagatc gaaatccgcg acgcgcttcg ggaatacgac gttggtgagt tcgagggcct
     3181 gccatatgcc gacgagcacg cctggcgatg ggatgcgtac gagcgggtcg accggtcgtg
     3241 gcaacggggc gaccgacagg cgtgcctgcc cggtggtgag agtctcgccg acatcgacgc
     3301 tcgcttcctg cccttcatgg aatcactcgc tgcgcggcac gctgcgagtg acaggctggt
     3361 gatcgtcagc cacggtgggc tctaccgagt tgcacttccg ggcctctgcc gcaccctgac
     3421 tgcgcacgat acccgacgcc taccgatgga ccactgtaca tcgatcatcg tcgtccacga
     3481 ggacggggac tggcgatgtc tcacgtggga tggcaagcat ccgccatttg catgcccctg
     3541 aacatgcgcg acgtgcgagc gcagccaggt gatcggcgcg gagggatcaa cgcgtggaga
     3601 gggaccaatg gcggcagaag cggaaggggg agacgccgag agcggaacca ggaaagtcat
     3661 ctacgccgcg ctgttcgcga atctcctgat cgcgattgcc aagttcatcg tcggcgcaat
     3721 ccaatacgag cttcgggtga tcgatccatg ggggaacact ttcgagggtc gactcggtca
     3781 ccggtcggca cgatgtggcc gcagtacggc taccgagcgg accacgccct gagggaggtc
     3841 cgcctctacc ggtattcgca cagcgttcaa ccgagttccg tacaaggtgc cgctgttcga
     3901 ggacgccgca ctcaccggcg tcctcatcgc ggccatcggc ctggggatcg tccagatcac
     3961 ggacaacagg gtgtacgacg gcgtggcgtc gatcggcatc ggtatagtgc tgatctacgt
     4021 cgccatccag ctgggagccg aattccgggc tctgctgatc ggcgaggcgg tatcaccgaa
     4081 cgatcgcgag cgcatgcgcg aggcgatgga atcgcccccg aggtgtcgac cgcgctgcgc
     4141 ctgctgacga tgcacttggg accccacgac gtgctggtga acgccgagat ccatctcagg
     4201 aacgagctcg acaccgacag aatcgagcgg ctgctggcgg acatcacccg gcggctgcgc
     4261 gaggagaacc caaaggtgag ccagacgttc atcgagctcc acgcgcccga ccagaaaccc
     4321 gccgcatggc aaacgacggg gggcggctga catccgttga gaaccgtgtc actccccaag
     4381 acaattgcgg gcatagagga tgaagttgcg tgccgcttgc gggtcgccca tacgctgacc
     4441 atgctcccag cgctcggccc gatcgacgag atcgatgatc cacgggtccg ggtatcgctc
     4501 ccgcacccat cgggctgcaa cgcgcttgga gaccacctcg tcgttttcag ctcgatgcag
     4561 gactcggcag atcgtcagcg tcacatatgc ctgctgatgg ctgctcgcga agaacgccct
     4621 gtcctccagc ttcggcaccc actcctccag caagtcgcgg cgtgacgcct cgcggacatc
     4681 ctcgatggcg attggcggga tcacccgctc aggatcgggg ccgatgagcg cgactccgca
     4741 cgaccgcagt gcgtagaggt tgatcaacca ctcgttgccg tatcggggat cagggtccca
     4801 gaacatgcca ccgttgatgt acggccgtgg ctccggtggc gggtcaaggc tgccgagcat
     4861 tgcctcggtg atgtacgacc cttcgatacg ctctgcccac tcgggaaatc gctgggcgat
     4921 cacggcgtgt gctgcctcca gtacggcgcg gaccgacgcc ggcatcggct gccgcatgat
     4981 cgcgaggtag tctatgtcgc tgctgccacg ctcgaagccg ccgtaggtca gggacccggt
     5041 gaggtagcat cccgtcagca cctcgcctgt gatctcgccg atacggcttg tcagctcgac
     5101 caggatagcg ttgatatccc ggtgcggcgt caattcgcta attgaatcgg gatccattgt
     5161 ctatcgctcc ctgccgtccc ccggctgttc ttcacagtcg attcgtgcta ttgactcctg
     5221 acatcagtga tcccctattt gcagcggctg gctccgcacc ttgatgctgt ccgcagcgag
     5281 gtgcggagcg agtggagcag cgcgccaagg gttctgtatg gattggttgc ttgacatcga
     5341 ttggcgaggc atgttcatcc cggatacggc gattgttgag ctcgtcatcc gcgggtcgat
     5401 catgtatctc gcgctgtact tcctgctccg cgttgtgctc aagcgacaga gcggaggcat
     5461 tggtatcacg gacctgctcg tgatcgtcct cattgccgat gcagcccaaa acgcaatggg
     5521 agacgactac cagtcgatcc cgaatggcat catcctggtg gcaaccatca ttttctggag
     5581 ctacaccctt gactggctcg gctaccgctt cccgcgcatc gaaccactgg tctacgccaa
     5641 accacttcag ctcgtcaggg agggccagat gcttcggcac aacatggagc gagagacgat
     5701 gaccgaaggc gagctcatga gtcagctgcg cctgcagggc gtcgaggaca tctcggtggt
     5761 caaggtagcc tacctcgagg gcgatggacg ggtgagcgtc attgtcgacg gtagcaagga
     5821 caagggagcc tctccaagac accgccccta gccaccctac ggccgtacgt ctgttgatag
     5881 ctacttggca acctggaacg ggtgatcgat cccgatacgg cgagccatct cggtcagctc
     5941 cctggccagc cccggcgcga ccggaatccc gttctcccgg ttgtcccgct ccgccgcgac
     6001 ctccgggtca cccggcacca gcaccgggcc cggcgcgtcg tcggcagccg gcgtctcacg
     6061 cagctcgcag accatctggt ccatcgcaac gatgaactcc tgaggatcac gaaaggcgtc
     6121 gatccgccat gccatgaaga agtgtcccgt gcccggcggg aaggtctgct cgaactggcg
     6181 attgacgtga ttgctccaaa ctccgccgga gagcggccca gtgaggatct cgaccatcaa
     6241 tccgagcccg tatcccttgt gaccggagcg ctcgcggatg cctccgagcg gggtcaaccc
     6301 gacaacctgg tgcggatcag tggttgtccg gccctcgtcg tcgacacccc acccctccgg
     6361 gatcggcagg ccagctcggc gggcgatctc gatcttgccc cacgccacag tgctggtcga
     6421 catgtccagc acgaagggct cggcggaggt cggcacggcg aaggcgatcg ggttggtgcc
     6481 gagccgggtg gttcgcccga agagcggtac caccactcgc gacgagttgg tcatcgccag
     6541 gccgccgagc ccctgcgtgg ccgccatcag ggcatagttg ccggcgatgc cgaagtggtt
     6601 gctgttgcgg acggtcgtca tgcagatccc gttggtctcc gctttggcga tggtccggcg
     6661 catcgcctcc gggccaacca ggagcccgag gccgagatgg ccgtcgagcg cgagcgtgac
     6721 cggcgactca cgcattacct ccacctcgac gtcggcacgg tcgaacccgc gctgcaggcg
     6781 cgagacatac ccggccagac gcccgaggcc gtgactggcg atgccgcgga gatccgattg
     6841 cagcagcagc ccccccgcct ctgccgcctg gtcctgggtc aacccggcgg tcacgaaggc
     6901 atcgatcgca aaggcgagca gggattcata cggatacctg gcgtctgaca cgagaacgga
     6961 gctccatggg ttccagcgcg tgcggacggt cgctgcgatc gccacagatg caacgaaact
     7021 acagtctcgc tgtcgattgt cgcaccgatc cgtcaaacgc caacagggtc cttcgcgctt
     7081 gacgcggcga tcaccggttg gataggattg gctatgacct tcgggcatgt tgcccgttcg
     7141 gttgtgtgcg ggtcgagcag tttgctcccc gttcgtgcca gtgaagggag cgtcgttgga
     7201 cgctggcagt cctcggtatc acgaacatac gagaccctgg ttcctgccgg accgacggcg
     7261 ctagccacat ggctgcaccc gctccggctg aggccggggc tatgactccg ggtcgctgtc
     7321 gccccggagc gagcaaggcg gtgcagcaat gcagaccaac cgaaaagccg tgcgattcta
     7381 catcgaccgc gacgccgaca ccccggtggt gcggtccgtt cccgtgtccc atccctcggt
     7441 cgctgccgtt tccgcgtcgc gggcgccgat cacccatcgc cccgaaaatg gcgacgagag
     7501 cgcgatggcg ttcgcagccg ccgagagccg ccgccgtggc gcgcggcatc ttctcactgc
     7561 cctccgcaag ccgcgacgaa atgcctcgtg acgtgacatg aggggctgcg cgctgaccga
     7621 ccgggctggg tctcatgact gagacccggc agccacctgc ccaggaaccg atcaccgatc
     7681 aggaggacga ggatgccccc ctgatcgagc agatccgcgt ggccgtggcc gagcgcgacg
     7741 tcgagcgagc ccgtggcttg gcccgtcgca tggtcgagga tgacgccctc gcgctgctgg
     7801 aggacctctc gcccgatgag ctgagccgcc tcttcgcctt cctcggcgac gagtcgctgg
     7861 cgttgctgct ctcccggctc gacgaccgcg acgccgccgg cattctcacg cggatgaccg
     7921 ccgcgcaggc cgccgacatc ctcgaagaga tcgaccccga cgacgcaacc gacatcttcg
     7981 ccgagatgga gcagtcggat ccggccgccg ccggcaccat cctcatcgag atggagccgg
     8041 acgaagctgc cgagatccgg gacctgatgg cctacccgcc ggacacagcc ggcggcatca
     8101 tgactccggc gttcgtggcc gtcttccccg atctccgcgc agacgagaca gtccgcgcgc
     8161 tgcgccaggt cgccgaagag gccgagacgg tcaactacgt ctatgtcatc tcacgggagg
     8221 acgagtcact gctcggcgtg ctgccactgc accggctcgt gctgagccgc cccaacgccc
     8281 cggtcggcga tctgatgacc ccggagatcc tctcggtgcc ggtccacgcc gaccaggaag
     8341 aggcggcgcg gctggtcact gagcacgacc tcctcgcgat cccggtcgtc gacgacgaca
     8401 accgcctgct cggcatcatc acctccgacg acgtcgccga cattctcgag gaagaggcta
     8461 cggaggatat cgagcggctg ggtggatccc aacccctcgc cgtgccgtac ctgcgcgcca
     8521 gccccttcct gctcttccgg cggcggatca tctggctgct cgtcctcttc atggcacagt
     8581 tcttcacggt cacgatccag gagaactacg ccgacgtgct cgaggaggtc atcctgctct
     8641 cagcgttcat cccgatcctg atcggcaccg gcgggaacgt cggctcgcag accgtctcga
     8701 cgatcatccg tgcgatggcg cttggcgagg tgcagccgca ccatatcctg cacgtggtcg
     8761 gcaaggaagc gatcaccggg gtggccctgg gcttggtgat gggactgctg atgttcttcc
     8821 gcgccgagct cagtgcggga agttccgtgc agatcggcct gacggtcggc gcgacggtgc
     8881 tggtgctcac catctgggcg gcaacggtcg gtgcgatcat ccccatcgtg ctgagcaagc
     8941 tgaagatgga tcctgcggtt gtgtccgcgc cgttcatcag cagcttcgtc gacggcacgg
     9001 gcctcatcat ctacttcacc ctcgctcgcg ccttcctgga cctctgagcg acgcccggcc
     9061 ctgccgggac tgctgattca agccgcccac tgcagcatca tcccgagcgc cgtgcagaca
     9121 gggcctccct cttcgcgcta cactcgttgt gccactgagg caagctcctt ctttaccgct
     9181 gtcgacgcgg ccccacgagt acccaggcag gtgtggacca tgcaaccgtc cgttgtcgcc
     9241 gtcatcaccg aagagaccca gaacgatatc gtcgccgcgc ttcacgcggc ccgggttggg
     9301 catctcacca gggtgatccg gcgagaacgg ggcgcgattg ccgcccagct cggccgtgcc
     9361 gggatcgaca ccgctgaact gccggccgcg attgacgctg ccgaccgcac cgtgcttatc
     9421 tcccccgcct cgcacgcgct cgaaaccgcc tgcctcgtgc tgcagcgcgg tgcggtgagc
     9481 gcttggacgc tcaccaacga cgatggctgg cagcccatcg acgacaaggc gatcgagatc
     9541 accgccacac gcggatttcc cgcccggccg gtcgctccag ctcaccgatc cggtcgcacc
     9601 ttccggaagc cgaacgtcca ccgccggccg cgccgagaca tcgtcacccc gcccaccagc
     9661 gc
//
LOCUS       DASYWL010000220         5119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_423460, whole genome shotgun sequence.
ACCESSION   DASYWL010000220 DASYWL010000000
VERSION     DASYWL010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5119
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423460"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1036)
                     /locus_tag="VGR22_07760"
     CDS             complement(83..1036)
                     /locus_tag="VGR22_07760"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEV2128497.1"
                     /translation="MAQTHPKYLWWNGTQMPWEDATVHVTDLAWSTVGAVFEGIRGYW
                     NAEEQELYIFRLPEHMQRLTESVRLVRLPLEYSAGQLIDATLTLLRDNDSREDTYIRP
                     LAYSANTSGKRISDANVELGMLINTNPMPSQLSSGRTLRLKVSSWTRISDNVMPPRIK
                     NISNYRNGQLASMEAQQDGYDNALLLGPHGKVSEAPGACVMMVRKGALITPPVTDGIL
                     ESITRDAVLRMASEALDMPVTERSIDRTELYLADEVFLCGTAFEVSPVVEIDHYTIGS
                     GEIGAFTGRLQALFEVVLRGTESRFAEWRTPVGVAAGAAAG"
     gene            complement(1177..2970)
                     /locus_tag="VGR22_07765"
     CDS             complement(1177..2970)
                     /locus_tag="VGR22_07765"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV2128498.1"
                     /translation="MSIRDHRDEQEALLKRLDHQMRMSAFTRRKLVAGSIVGAGALAL
                     GGLAGSKAVMAQDAAGELDEHQILYNAALFKDPLSFDWNANLYVNADVSTFSGLLTFN
                     PDGEAIGDWAETWESNEDASVWTFHLREGNNGWTNGDPVTAHDFVWSFQRILDPETAN
                     VYAFILYDLLHGEAFNTGTEVEIDGETRVPTAEDLGLKAIDDWTFEMTLEGPRANLPQ
                     KVAYLACYPAHRPSVEEHGEQWALGDVPLVSNGPFKLDLWEKGVKIELSKNENHWYAE
                     EITLEKVIDPLIPATNHVNAFEHGTGDQQLDWVNLGASDYVRFQEDPEKAEMLRQYVY
                     PGTWFLLPSNGIPPFDQIEVRRAVSQAIDRNRLVEVTQGLASPAHCMVPQGVYGFFED
                     EEIYAIQAFDPESALAHLEGTEFEGGQNWPEITMIMRANEELYNSDIMANDIVAQLEE
                     NLGMQIQIETLVENSFRPRLYENRDQLVWIRWWYDYPDPDNGYYDMFYGGKPVGSRRQ
                     AWTDDEYDRIVVEAKSVLDPEARLELYKEAERIIQTEVGYVPLVYRLDVYAFKPWVQN
                     IPVNEQGFTVPDGNIYVRALTQYQISGRPQE"
     gene            complement(3153..4163)
                     /locus_tag="VGR22_07770"
     CDS             complement(3153..4163)
                     /locus_tag="VGR22_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2128499.1"
                     /translation="MTKGETGVARNQTDNTAAVAPGASISKETVDTKDASAVGTTPLA
                     RLAAGPVPRQRSLWTDAWRRLAKNKMAVVGLIIVVAFTLMAALAPLLAPYSQAEVVHV
                     GLAREGPSWLWPLGLDQNGRDIWSRLLYGARVSLLVGVVSQLIVLAIGVPVGAVAGFM
                     GGNIDNLLMRFVDVFYAVPQLLLVLIFLNLFGPGLLNIFLAIGLVSWVTIARLVRGTF
                     LSLREAEYVHAARVSGAGDGYIMLRHMLPNSLTPIIVAVTFGIPTAIFTEAALSFVGV
                     GINPPQASWGQMVGVGTDTGLVQASPHILLFPVAAIGLTMLGFTFLGDGLRDALDVRS
                     KD"
     gene            complement(4175..5113)
                     /locus_tag="VGR22_07775"
     CDS             complement(4175..5113)
                     /locus_tag="VGR22_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2128500.1"
                     /translation="MLAYILNRILWFIPTLLVITLLTFLILQATPGTGVISSGAQIVD
                     QRVVERLEAEYGLDEPLYQQFGIYVWNVLNGDFGTSFVYRPQTVNDIIGRTLPISLQL
                     GVYATILAVVVGVGLGVLSAVHQNGVIDYFSVGVAVLFYSLPNFVMGFVLILVFVVYL
                     PRIGIDMAFNVGGLDSWKDWILPTIALAATPLATIARFTRSSMIEVLQSDFVRVARAK
                     GLRENKVIMRHVIKNALIPIITLIGPIFAAVATGSFFVEAVFNIPGMGRYYVESFVAK
                     DQPMILAVTLIYAVMLAAMNVVVDVLYGFIDPRIRY"
BASE COUNT          973 a         1621 c         1609 g          916 t
ORIGIN      
        1 actacgtggc cggaatcacg gatgcgtcgg gccagtttgc cctgctccgg tctggcccgg
       61 ccccgtctga gtggacctca cgctagcctg ccgcggcccc ggccgcgacg ccgaccggcg
      121 tgcgccactc ggcgaaccga ctctccgtcc cgcgcagcac cacctcgaag agcgcctgca
      181 ggcgtccggt gaacgcaccg atctcgccgc tgccaatcgt gtagtggtcg atctcgacga
      241 cgggactcac ttcgaaggca gtcccgcaga ggaagacctc gtcggcgagg tagagctccg
      301 tgcggtcgat ggagcgctcc gtcaccggca tgtcgagcgc ctcactggcc atgcgtagca
      361 cggcgtcgcg cgtgatactc tcgaggatgc catcggtcac cggcggggtg atgagagccc
      421 ccttgcggac catcatcacg caggcaccgg gcgcctcgct caccttgccg tgcggcccga
      481 gcagcagcgc gttgtcgtag ccgtcctgct gcgcctccat gctggcgagc tgaccgttac
      541 ggtagttcga gatgttcttg atgcggggcg gcatgacgtt gtcggagatg cgcgtccagg
      601 agctgacctt gagccggagc gtgcggcccg aggagagctg agagggcatc gggttggtgt
      661 tgatcaacat gccgagctcg acattggcgt cggagatgcg cttgcccgag gtattggcgg
      721 aataggccag cggccggatg taggtgtcct cgcgcgagtc gttgtcgcgc agcagggtca
      781 gcgtcgcgtc gatcagctgc ccggcgctgt actccagcgg cagacgaacg aggcgcaccg
      841 actccgtgag ccgctgcatg tgctccggca gccggaatat gtagagctcc tgttcctcgg
      901 cgttccagta gccgcggatg ccctcgaaca ccgcgccgac cgtcgaccac gccaggtcgg
      961 tgacatggac ggtggcgtcc tcccacggca tctgggtccc attccaccag aggtacttcg
     1021 ggtgcgtttg cgccattgcg agcgtccttt cgcagtcgat tcacgtgggt cgccgtcgac
     1081 cgatgagccg cagtatcgca tggcccccag gcatgggaaa gggcggccgc ttcggcggcc
     1141 accccttcca cgtcggtcac ttgcccaccc gctcggctac tcctgcggcc gcccggagat
     1201 ctggtactga gtcagggccc gaacgtagat gttgccgtcc ggcaccgtga agccctgttc
     1261 attaaccggg atgttctgca cccacggctt gaatgcatag acgtcaagcc ggtaaacgag
     1321 aggcacgtag ccaacctccg tctggataat gcgctcggcc tccttgtaga gctcgaggcg
     1381 cgcctccgga tcgaggacac tcttcgcctc gaccacgatg cggtcgtact catcgtccgt
     1441 ccacgcctgg cgccgagaac cgaccggctt gccaccatag aacatgtcgt agtagccgtt
     1501 gtccggatcc gggtagtcgt accaccagcg gatccagacg agctggtcgc ggttctcgta
     1561 gaggcgcggc cggaagctgt tctccaccag cgtctcgatc tggatctgca tgcccagatt
     1621 ctcttcgagc tgggcgacaa tgtcgttggc catgatatcg gagttgtaca gctcctcgtt
     1681 ggcgcgcata atcatcgtga tctcgggcca gttctggccg ccctcgaact ccgtcccctc
     1741 gaggtgcgcc agcgccgact cggggtcgaa cgcctgaatc gcgtagatct cctcgtcctc
     1801 aaagaagccg tagacaccct gcggcaccat gcagtgggca ggcgacgcca ggccctgcgt
     1861 gacctcaacc agccggttac ggtcgatcgc ctggctcacg gcgcggcgaa cctcgatctg
     1921 gtcgaacggc gggataccgt tagacggcag caggaaccag gtgcccgggt agacgtactg
     1981 ccgcagcatc tccgccttct ccgggtcttc ctggaagcgc acgtagtcgc tcgcgccgag
     2041 gttcacccag tcgagctgct ggtcaccggt gccgtgctcg aaagcattga cgtggttcgt
     2101 ggcggggatc agcgggtcga tgaccttctc cagcgtgatc tcttccgcgt accagtggtt
     2161 ctcgttcttc gacagctcga tcttgacacc cttctcccac aagtccagct tgaagggacc
     2221 gttggagacg agcggtacgt caccgagcgc ccactgctct ccgtgctcct cgacggaggg
     2281 acggtgagcc gggtagcagg ccaggtaggc gaccttctgc ggcaggttgg cgcgcggacc
     2341 ctcgagcgtc atctcgaacg tccagtcgtc gatcgccttg aggccgaggt cctccgcggt
     2401 cggaacccgc gtctccccat cgatctcgac ctcggtgccg gtattgaacg cctcgccatg
     2461 gagcaggtcg tacaggatga atgcgtaaac gttcgcggtc tccggatcga ggatgcgctg
     2521 gaacgaccaa acgaagtcgt gggcggtaac cgggtcaccg ttggtccagc cgttgttgcc
     2581 ctcgcgaagg tggaaggtcc agaccgaggc gtcctcgtta gactcccatg tctcggccca
     2641 gtcgccgata gcttcgccgt ctgggttgaa cgtgagcaga ccggagaatg tcgaaacatc
     2701 ggcgttgacg tagagattcg cgttccagtc gaaggaaagc gggtccttga acagcgcggc
     2761 gttgtagaga atctgatgct cgtcgagctc acctgcggca tcctgtgcca tcacggcctt
     2821 gctgcccgcc aggccgccga gggcgagggc acccgcaccg acgatcgagc cagcgacgag
     2881 cttgcggcgc gtgaaggccg acatgcgcat ctggtgatcg agccgcttca gcagtgcttc
     2941 ctgttcgtcc ctgtgatccc gtatggacat ttattgcagc ctttcaggtt ttgtcctatt
     3001 tcggtggcgg gcattcgccc accccacgag cttgtctggc gtaccgcgtc tcgaactcgc
     3061 atgagcccag tacgtcgctg ccagtcgaac gcgcctcctt tcaaccgttg caaatccaaa
     3121 cgaacctggc aagcggattg tcgcgggtcg tatcaatcct tgctacgcac gtcaagcgcg
     3181 tcacgcagcc catcaccaag aaaggtgaag ccgagcatag tcagaccgat cgccgcgacc
     3241 gggaacagca ggatatgggg cgaggcttga accagcccgg tatctgtccc aacaccgacc
     3301 atctgccccc atgatgcctg aggcgggttg atacccacgc cgacgaagct gagtgccgcc
     3361 tccgtgaaga tcgcggtggg aatgccgaag gtcacggcaa cgatgatggg tgtcaggctg
     3421 ttgggaagca tgtgccggag catgatgtac ccgtcccccg ctccggagac gcgggcggca
     3481 tggacgtact cggcctcgcg cagcgagagg aacgtgccgc gaaccagcct ggcaatcgtc
     3541 acccagctga ccaggccgat agccaggaag atattgagca ggccagggcc aaagaggttc
     3601 agaaagatca acacgagcag gagctgcggc accgcgtaga agacatcgac aaagcgcatg
     3661 agcaggttgt cgatattacc gcccatgaag cccgcgaccg cacccaccgg cacgccgata
     3721 gcgagcacga tgagctgtga cacgacaccg accagcagcg agacccgtgc gccgtagagc
     3781 aggcggctcc agatgtcacg gccgttctga tccaggccga gcggccacag ccagctcggc
     3841 ccctcgcgag cgagcccgac gtggaccacc tcggcctgcg agtacggcgc cagcagtggc
     3901 gccaaagcgg ccatgagcgt gaacgccacg acgataatca gcccgaccac ggccatcttg
     3961 ttctttgcca agcggcgcca tgcgtccgtc cacaggcttc gctgacgggg aaccggccct
     4021 gcggccagcc tcgccagtgg cgtggtaccg acagcggagg cgtccttcgt gtcgacggtc
     4081 tctttactta tcgatgcgcc cggggccacc gcagcagtgt tgtcagtctg atttcgggcc
     4141 acgccggttt cgcctttcgt cacaggtcag agtgctagta gcggatgcgc ggatcaatga
     4201 agccgtagag cacgtccacg acaacgttca tcgctgcgag catcacggca tagataaggg
     4261 tgactgccag aatcatgggt tgatccttgg caacgaagct ttccacatag tagcgcccca
     4321 tgccagggat gttgaagaca gcttcgacaa agaacgagcc cgtcgcgact gctgcgaaga
     4381 tcggcccgat gagtgtgatg attggaatga gcgcgttctt gatcacatgc cgcatgatca
     4441 ccttgttctc gcggagcccc ttcgcccgcg ctacgcgaac gaaatcgctt tggagcacct
     4501 cgatcatgct cgaccgcgtg aacctggcaa tcgtggcaag cggggtcgct gcgagggcga
     4561 tggtcggcag gatccagtcc ttccaggagt cgagcccacc gacgttgaac gccatgtcga
     4621 tgccgatgcg cggcaggtag accacaaaca ccagaatcag cacgaagccc atcacgaagt
     4681 tcggcagact gtaaaagagc accgcgacac cgacggagaa gtagtcgatg acgccgttct
     4741 ggtgaaccgc cgagagtaca cccaggccga caccgacgac gacggcgagg atcgtcgcgt
     4801 agacgcccaa ctgcagcgag atcggcaacg tacggccaat gatgtcgttc accgtctggg
     4861 gacggtaaac gaacgaggtg ccgaaatcgc cgttgagcac gttccagacg tagatcccga
     4921 actgctggta gagcggctca tcgaggccgt actctgcctc gagccgctcg acaacgcgct
     4981 gatcgacaat ctgtgcgccg gacgagatca cgccggtgcc gggcgtggcc tggagaatca
     5041 gaaaggtaag cagcgtgatg accaggaggg tgggaatgaa ccagaggatt cggttgagga
     5101 tatacgcgag catctaccc
//
LOCUS       DASYWL010000221         8239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_310510, whole genome shotgun sequence.
ACCESSION   DASYWL010000221 DASYWL010000000
VERSION     DASYWL010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8239
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310510"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /gene="ssrA"
                     /locus_tag="VGR22_07780"
     tmRNA           <1..214
                     /gene="ssrA"
                     /locus_tag="VGR22_07780"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(368..853)
                     /locus_tag="VGR22_07785"
     CDS             complement(368..853)
                     /locus_tag="VGR22_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEV2128501.1"
                     /translation="MANQQYWVLVSSADNFQATKDRGFTVQGIKSRHRKKAEQMKPGD
                     KLVYYLTGRKVFAGTATVTSEYFEGDDLIWMSKNRKKDGSPENYPFRVEIEPEVVADE
                     DTWIDAEPVARQMIHARKWPEQNWTLAFQGNVHNVPVEDYELIRGLLEEAINDAKSGA
                     D"
     gene            1038..2498
                     /locus_tag="VGR22_07790"
     CDS             1038..2498
                     /locus_tag="VGR22_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEV2128502.1"
                     /translation="MDRQHFISELESICGAANVRSRPADLLVYEYDGSVDGAVETERP
                     LAVVLPISTDQVAAVVRLAHRHGVPVVARGAGTGLSGGAVARGGGVIIALTRMERVLR
                     VDPVSRTALVEPGVINLELSQTVAGEQLFFAPDPSSQKACTIGGNVAENSGGPHCLKY
                     GVTTNHILGLEVVLPDGDVVWLDSNGPGIAGLDLAAILVGSEGMLGIVTKALVKLMPS
                     PEAVHVHLAAFGTMDDAAAAVTNIIGAGILPASLEMMDHLTIEAVEPAYHAGYPMDAA
                     AVLLVEVDGTRQEVADTSERIDRLVREGGATSFRSAEDSVEQDLLWKGRKMALAAMGR
                     LAPNYYLHDTVVPRTRLMEMLRSVNDISIIHQLPIANVFHAGDGNLHPLMLFDRRKPG
                     DVERILEASHALIHSTIEMGGALSGEHGIGSEKRGYMELVYTHDDLCAMAGVKDAFDP
                     RSLLNPEKVLPKGYMCGEVRALHNQAMAQKHGIHPM"
     gene            2514..3734
                     /locus_tag="VGR22_07795"
     CDS             2514..3734
                     /locus_tag="VGR22_07795"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEV2128503.1"
                     /translation="MTQKRMIDGVLPGTWARPESPEGVAEAIHHAGLRGQWLLPVGGG
                     TAQNAANPVDVIPVALDTTGLTGIRDYEPTDLTVSVASGTRWTDLQSTLGENGQQIPV
                     DVPFPDIATVGGVVAMGYAGPRRLRDGTLKDLLLGASFVRGDGLVAKAGGMVVKNVSG
                     FEIARLLHGSWGSLAVITSVNLKVIPRPEFELTLLANELDTLEGAERVLRLTRSRSTI
                     AAATVDGTLERATVAVRLTGREQPTLELAREVKSAASLGWRDDPIDGEASAEWWQARE
                     DRLAGGDGRVSIEVGCQPSNAVETLRTLRSALPVKSLEFHASPGAGAIELAFDAEALA
                     LSTWTRIWTEHGLDTTARFTVGSAPPSWRAERDVWFIPAAQRKLMAALKDAFDPQNML
                     NRGRLWTDSPTNQL"
     gene            3753..5156
                     /locus_tag="VGR22_07800"
     CDS             3753..5156
                     /locus_tag="VGR22_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEV2128504.1"
                     /translation="MATQASSINQTLLEAERTMHGYSGHDVPDDKLLRDCVHCGMCLP
                     TCPTYRLTGSEASSPRGRLWMMKSVADGRMDLLDPEFDEQMYQCLNCRACEAVCPSGV
                     QYGPLVEAARAQLEQHRPRPLWQRALRIALMRVPFDDVRRMRLGVGGLRLYQKSGLSA
                     LLRRTGVLRRLRLETLEAMVPPLEEPALLSGEESWMPDQAPSRAHLFNGCVMSTIFSN
                     VNRAAGRSLAHNGYATDVPARQQCCGALYVHSGMMNEARRLAKTNIAAFEEAGEGPVV
                     VTAAGCGAALKEYGHLLKEEPAWHERAEAFAERVVDLTELLGTEKLVQPGEEVEMHVS
                     YQEPCHLAHAQRITRQPRSILQAIPGLELTEMRESSLCCGSAGIYNIIRRKMADDLGD
                     RKASAIRDTGARTVITANPGCHMQLRTSLRRNDIDVEVRHIAQVLDEAYGGDRAARCG
                     DWAFRRESGAAQLPARD"
     gene            complement(5135..7033)
                     /locus_tag="VGR22_07805"
     CDS             complement(5135..7033)
                     /locus_tag="VGR22_07805"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV2128505.1"
                     /translation="MPRVHRTFDSILSILVGVASGATTVQEAGDQLIALHAGAQATAT
                     ALCRDVAAALVEMESEGEIAQEDSRAILGRLASDSPEPRHHFGKLKEVVQAAAGSKPL
                     TERLTHVSHLVTDATGMDACTLFLLDDATHTLALRAATGMDPSTVGAVTLRVEQGIVG
                     RAAREWQTIAASHATEHEDYLNHPGLTDERFSSQLSSPLIVTEQQRLVGVISVHALET
                     KEFSKDDIEFLETIAGMLAVSIDSSRQSARTDDRLQQKIVELTTLQRVSRVVASSLNL
                     TEVLKLITEQAVELVQAEAAAIFRASRDLQPSEEPRPSIEYRVGVIRSHENERERDFV
                     VRDVLRTGVARTRDIAYRDGMSTVFCLPLNTARETVGSLCFRMRQGTALDEEQLGLLQ
                     AFGDAAALAIDNAQLYQEAVESVRTQSALVQEMHHRVRNNLQTVAALLSLQQRAEEGN
                     PWSPELGEAVSRIQAIAGVHDLMSDESRLAGTTVDALARMVAEEAHGTLTPPDLRVEF
                     EIEKSNLRVPSRQATIIALLINELTANAIHHGFRSRSRGKIRISAREVQGVAVLQVAN
                     DGEQISPDFDPMRSRGLGMRITKQLVTSDLHGEFTIMPTVEGTLATIRFPITKLTQDE
                     LGQSLAGS"
     gene            complement(7044..>8239)
                     /locus_tag="VGR22_07810"
     CDS             complement(7044..>8239)
                     /locus_tag="VGR22_07810"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEV2128506.1"
                     /translation="TGFGRYTRVWTVKTDGSELTNHTADVDVTFSDTGMSDLATGNGT
                     PPVWIDNATLVVSGSARGATSIYRIPLGKGEAEAITAENRRVLQFAVSDDGKTLVYVA
                     GAIDQPFRLFASTVRGKRKTVLHDPNEALVSEVEITAPIELDVTAPDGGDVQTWLLPP
                     HGLKPEGQVKYSLIVQIHGGPHSMYGHGMFHEMQVMAGRGYGMLFCNPRGSSGYGEAF
                     AIGTRGAWGESDMPDVIAAVDEASKLDWVDSERLGVTGGSYGGYLTNWIISHDDRFKA
                     AVTQRCVSNFHSFVGTSDIGFDFGIYEFDGTPWKDAEKLLRYSPISFVDHIQTPLLII
                     HSEQDIRCPIEQAEQMYTALRYLDREVAFVRIPEEGHELSRSGTPSRRVARLHHLIGW
                     FDSHL"
BASE COUNT         1441 a         2586 c         2702 g         1510 t
ORIGIN      
        1 gtcctcactc cgtcgggacc ggaactgggc gccaccaaga cggagtgctg cgatctagtt
       61 cgccggagcg cgatcgcaaa agatgagtct ggcttaccgt ccgttggtcc tgtcggcggg
      121 gcattcgggc gggaaaccca aacaccgact aagcgtgtag tagatgtccg gtattcggtt
      181 ctggacgggg gttcgactcc ccccatctcc accgaagcgc atgagggcgg tccagatgga
      241 ccgccctcgg tgtttctgca ggtttcgtgt cactcggggg aaccggactc agccgccgtg
      301 cccgggcgcg cccctccagc tcggcatggc gggcgtatga gcgaaaatct ccctgcgcca
      361 gccaaaccta gtccgcgccg ctcttcgcgt cgttgatcgc ctcctcgagc aagccgcgga
      421 tcagctcgta gtcctctact ggaacgttat gcacgtttcc ctggaaggcg agcgtccagt
      481 tctgttcggg ccacttcctg gcgtgaatca tctgccgggc cactggctca gcgtcgatcc
      541 aggtgtcctc gtccgcgacc acctctggct cgatttcgac acgaaacggg tagttctccg
      601 gcgagccgtc cttcttcctg ttcttgctca tccagatcag gtcgtcgccc tcgaagtact
      661 cgctcgtcac ggtcgcggta ccggcgaaca ccttcctgcc cgtgaggtag tagaccaact
      721 tgtcgcccgg cttcatctgc tcggctttct tgcgatgccg ggacttgatg ccctgcacag
      781 tgaagcctcg gtcctttgtg gcctggaagt tatctgctga gctcacgagc acccagtact
      841 gctgatttgc cattgacgga gttgaccctc tcactctgct ggaagtgtac agtcggcacg
      901 gcccgctggc gggtatgatg gctgcaagaa tgccgtgaga cccacgctat gtcactgctg
      961 ttgacgcgta tgtgcggttt tccgatactg ggtgttcacc gacgcgctcc tgcatgccac
     1021 ggaaaggcca tcgcatcgtg gaccgtcaac acttcatctc cgaactcgaa tccatctgtg
     1081 gcgccgcgaa tgtccgctcc cggcccgccg atctgctcgt ctacgagtac gatgggtcgg
     1141 ttgacggcgc tgtcgagacc gagcggccgc tcgcggtcgt tctcccaatc agtaccgacc
     1201 aggttgccgc cgtggtccgg ctggcgcacc gccatggggt gccggtggtc gcccgcgggg
     1261 ccgggacagg gctgtcgggc ggcgccgtgg cgcgtggagg cggcgtgatc attgccctga
     1321 cgcgcatgga gcgggtgctc cgggtcgacc cggtcagtcg cacggcgctc gtcgagccgg
     1381 gagtgatcaa cctcgagctc tcgcagacgg tcgccggcga gcagctcttc ttcgcacccg
     1441 atccgtccag ccagaaggcg tgcaccattg gcggtaacgt tgccgaaaac tccggtggtc
     1501 cccactgtct caagtacggc gtgacgacca accacatcct cggcttggag gtggtgctac
     1561 cggacggcga tgtcgtgtgg cttgactcca acggccccgg catcgcgggg ctcgacctgg
     1621 ccgcaatcct cgtcggctcg gagggcatgc tggggatcgt caccaaggct ctggtcaagc
     1681 tgatgccatc accggaagcc gtccacgtcc atctggccgc attcgggacg atggacgatg
     1741 ccgccgcggc ggtgacgaac atcatcggtg ccggcatcct gccagcgtcc ctcgagatga
     1801 tggatcacct cacgatcgaa gcggtcgagc cggcctacca cgccgggtat ccgatggacg
     1861 ctgccgcggt actgctggtc gaggtggacg gcacgagaca ggaagtcgcc gacacgagcg
     1921 agcggatcga ccggctggtg cgtgagggcg gcgcaacctc gtttcgctca gccgaggata
     1981 gtgtcgagca ggatctgctc tggaaggggc ggaagatggc gcttgcggca atgggacgac
     2041 tggcgcccaa ctactacctg cacgacaccg tggtgccacg gacgcggcta atggagatgc
     2101 tgcggtcggt caacgacata tccatcatcc accaactgcc gatcgcaaac gtcttccacg
     2161 ccggcgacgg caacctgcac cccctgatgc tcttcgaccg acgcaaaccg ggcgatgttg
     2221 agaggattct ggaggcgagc cacgcgctca ttcactccac tatcgagatg ggcggcgcgc
     2281 tttccggtga gcacggcatc ggcagcgaga agcgcgggta catggagctg gtgtacacgc
     2341 acgacgatct gtgtgcgatg gccggcgtga aggacgcgtt cgatccccgg agcttgctca
     2401 atcctgagaa ggtgcttcca aaggggtaca tgtgtggcga ggtgcgggcg ctgcacaacc
     2461 aggcgatggc gcaaaagcat ggcatccacc cgatgtagag gggcaattgc tggatgacgc
     2521 agaagcggat gatcgacggc gttcttccgg gcacgtgggc ccggcccgaa agcccggaag
     2581 gggtcgccga agcgatccat cacgccgggc tccgtggaca atggctgcta ccggttggcg
     2641 gggggacagc gcaaaacgcc gcgaacccgg ttgatgtgat tccggtggca ctcgatacca
     2701 caggtttgac cggcatccgt gactacgagc cgacggactt gacggtctcg gtggcgagcg
     2761 gcacgcgatg gacggacttg cagtccacgc ttggcgagaa cgggcagcag atccctgtcg
     2821 atgtgccatt cccggatatc gcgaccgtag gtggtgtggt cgcgatgggc tatgcagggc
     2881 cgagacggct ccgtgacggc acgctgaaag acctgctgct tggcgcctcc ttcgtgcgcg
     2941 gcgatggtct cgtcgcgaag gcgggtggta tggtcgtcaa gaacgtcagc ggattcgaga
     3001 tcgcgcgact gctgcacggc tcgtggggct cgctggcggt catcacgtca gtcaacctga
     3061 aggtgatccc acgtcccgag ttcgagctga cgcttctcgc gaacgaattg gacacgctcg
     3121 aaggcgcaga gcgcgtgctc aggcttactc ggtcccgcag cacgattgcg gcggcgaccg
     3181 ttgatgggac gttggagcga gcaacagtcg ccgtccggct gaccggtcgc gagcagccga
     3241 cgctcgagct ggcccgcgag gtgaagtctg ccgcttctct tgggtggcgc gacgacccga
     3301 tcgatggaga ggcgtcggcg gagtggtggc aggcgcgaga ggatcggctc gccggcggcg
     3361 acggccgcgt gtcgatagag gtaggctgcc agccgtccaa tgcagtagag acactcagaa
     3421 cactccggag cgcgctgccc gtgaaatcgc tcgagttcca cgcctcgcca ggcgcggggg
     3481 ccatcgagct ggcgttcgat gccgaggccc tggcgctcag cacatggacg cgcatatgga
     3541 ctgagcacgg gctcgatact acggcgcggt tcaccgtcgg ctcggcaccg ccatcgtggc
     3601 gcgccgagcg agacgtgtgg tttatcccgg cggcgcagcg caagctcatg gcggcgctca
     3661 aggacgcctt cgatccccag aatatgctca atcgcgggcg gctctggacc gactcgccga
     3721 ccaatcagtt gtgatgctgg agaatgactc gcatggcgac gcaggctagt tcgatcaacc
     3781 agacgctact ggaggccgag cgcacaatgc acgggtactc cggccacgac gtgccggacg
     3841 acaagctgct gcgcgactgc gtgcactgcg gcatgtgcct gccgacctgt ccgacctacc
     3901 ggctcacagg cagcgaagca tcgtctcctc gcggacggct ctggatgatg aagtcggttg
     3961 ccgacgggcg catggacctg cttgatcccg aattcgacga gcagatgtat cagtgcctga
     4021 actgccgagc ctgtgaagct gtctgcccat ccggcgttca gtacgggcca ctcgtcgagg
     4081 cggcgcgcgc tcaactcgag cagcaccgac cgcgaccgct ctggcagcgc gctctgcgaa
     4141 tcgcgctgat gcgggtgccg ttcgacgacg ttcgtcggat gcgactcggc gtgggcggcc
     4201 tgcgcctcta tcagaagagt gggctttcag cattgctgcg acggaccggt gtgctgcgcc
     4261 ggctccggct cgagacgctg gaggcgatgg tgccaccact cgaggagcca gcactcctct
     4321 cgggtgagga gtcctggatg ccggaccaag cgccttcgcg ggcgcatctc ttcaacggtt
     4381 gcgtgatgag cacgatcttt tcgaatgtca atcgcgcggc tggacgctcg ctcgcacata
     4441 acgggtacgc cacggatgtg ccagcccgcc agcagtgctg cggcgcactc tacgtgcact
     4501 cgggcatgat gaacgaggca cgccggctcg ctaagaccaa catcgcggcg ttcgaggaag
     4561 ccggggaggg gccggtcgta gtcaccgccg cgggatgcgg ggccgccctc aaggagtacg
     4621 ggcacctgtt gaaagaggag ccggcctggc acgaacgcgc ggaggcgttc gccgagcggg
     4681 tcgtcgatct caccgaactg ttgggtacgg agaagttggt ccagccaggc gaagaggtgg
     4741 agatgcacgt ctcctatcag gagccgtgcc atctcgccca cgcacagcgc atcacgcgcc
     4801 aaccgcgatc gattctccag gcgatccctg gtcttgagct aaccgagatg cgtgaatcgt
     4861 ccctttgctg tggcagcgct ggcatctaca acatcattcg tcgaaagatg gcagacgatc
     4921 tcggcgaccg gaaggcgtcg gcgatccggg acaccggtgc caggaccgtc attacggcca
     4981 atccgggctg ccacatgcag ctgcgaacgt ccctgcgccg caatgacatc gacgtcgagg
     5041 tgcggcacat cgcgcaggtg ctcgatgagg cgtacggtgg tgatcgggcg gcacgatgcg
     5101 gcgattgggc gtttcgccgg gagtccggtg cggctcagct gcccgccagg gactgaccga
     5161 gctcgtcctg agtgagtttg gtgatgggga aacggatcgt tgccagcgtg ccctcgacgg
     5221 tcggcatgat ggtgaactcg ccgtgcaggt cagaggtcac cagttgcttg gtgatgcgca
     5281 tgccgagtcc ccggctgcgc atcgggtcaa agtccggcga aatctgttcc ccgtcgttgg
     5341 cgacctgaag cactgcgaca ccctgcacct cgcgtgctga tatccggatt ttgccgcgcg
     5401 agcgactgcg aaagccgtgg tggatagcat tggccgtcag ctcgttgatg agcaacgcga
     5461 tgattgtcgc ttgccgcgac ggcacgcgaa ggttcgactt ctcgatctcg aactcgactc
     5521 gcaggtccgg aggggtaagg gtcccgtgcg cctcctcggc gaccatccgt gccaaggcat
     5581 ccacggtcgt acccgccagt cgcgactcgt cgctcatcag gtcgtgcacg ccggcgattg
     5641 cctggatgcg gctgaccgcc tcgcccagct ccggactcca tgggttgccc tcttcggctc
     5701 gttgctggag tgagagcagc gcagcgaccg tctggaggtt gttgcgaacg cggtggtgca
     5761 tctcctggac gagcgccgac tgcgtccgta cgctttccac ggcttcctgg tagagctgcg
     5821 cgttgtcgat cgcgagggca gcggcgtcac cgaaggcctg cagcagcccg agctgctcct
     5881 catccagtgc agttccctgg cgcattcgga aacataggga tccgaccgtc tcgcgggccg
     5941 tgttgagcgg caggcagaat acggtactca tgccgtccct atacgcgatg tcccgagtgc
     6001 gcgccacacc cgtgcggagg acatcacgca cgacgaagtc tcgttcgcgc tcgttctcgt
     6061 ggctgcgtat gaccccaacc cggtactcga tcgacggccg aggctcttcg gatggttgca
     6121 ggtcgcgact cgcacggaaa atcgccgccg cctcagcctg taccagttcg acagcctgct
     6181 cggtaatgag cttgagcacc tcggtgagat tgaggctgga agcaacgacg cgtgagaccc
     6241 gctgcagcgt ggtcagctca acaatcttct gctgaaggcg gtcgtcggtg cgggctgact
     6301 ggcgagagct gtcgatcgac accgcgagca taccggcaat cgtctccagg aactcgatgt
     6361 cgtccttgct gaactccttc gtttccagag catgcacgct gatgacgccg actaggcgct
     6421 gctgctcagt tacgatgagc ggtgacgaaa gctgggagga gaacctctcg tcggtcaggc
     6481 ccgggtggtt gagataatcc tcatgctccg tcgcgtgcga agcggcgata gtctgccact
     6541 ccctcgccgc ccttccaacg atgccctgct cgactcgcag ggtgacggca cccacggtgg
     6601 acgggtccat tccggtggca gcccgtagcg ccagcgtgtg cgtcgcgtca tctaagagaa
     6661 agagggtgca ggcatccatc cccgtcgcgt cggtgacgag gtgggacaca tgggtcaggc
     6721 gctcagtcag cggcttgctg cctgccgcgg cctgcacgac ctccttcagc ttgccgaagt
     6781 gatgccgcgg ctcgggagaa tcggaggcga ggcgacccag gatcgcccgt gagtcctcct
     6841 gcgcgatttc gccctcgctc tccatttcga ctagcgcggc agcgacatct cggcagagcg
     6901 ccgttgcggt cgcctgtgcg ccagcatgca atgcgatcag ttgatcgccg gcctcctgaa
     6961 ctgtggtcgc acccgatgcc acgccaacaa ggatcgacag gatcgagtcg aaggtgcggt
     7021 ggacgcgcgg catggtgccc ttcctaaaga tgcgagtcga accacccgat gaggtggtgg
     7081 aggcgagcaa cccgccggct cggcgtgccc gagcgcgaca actcgtgccc ttcttcagga
     7141 atccgaacga aggcgacttc ccggtcgagg tagcggagcg ccgtgtacat ctgctctgcc
     7201 tgctcgatcg gacagcggat atcctgctcg ctgtgaatga tcagcagtgg cgtctgaatg
     7261 tggtccacga agctgatcgg cgaatagcgc agcagctttt cagcatcctt ccacggcgtg
     7321 ccgtcgaact catagatgcc gaagtcgaag ccgatatcgc tcgtgccgac gaaggagtgg
     7381 aagttggaga cacatcgttg ggtgactgcc gccttgaagc gatcgtcatg gctgatgatc
     7441 cagttggtca ggtacccgcc gtaggagccg cccgtcacgc ccagccgctc ggagtcgacc
     7501 caatccagct tcgacgcctc atcgaccgct gctatcacat ccggcatgtc gctctcgccc
     7561 cacgcgccac gagtgccgat cgcaaacgcc tcgccgtaac ccgacgagcc acgggggttg
     7621 cagaagagca tgccgtagcc gcgccccgcc ataacctgca tctcgtgaaa catgccatga
     7681 ccgtacatcg aatgcggccc gccgtggatt tgcacgatga gggaatactt gacctgacct
     7741 tcgggcttga gaccatgggg cggaagcagc caggtctgaa cgtcgccacc atccggtgca
     7801 gtgacgtcga gttcgatggg agcggtgatc tcgacctcgc tcaccagcgc ctcattcggg
     7861 tcgtgcagga cggtcttgcg cttgccccga accgtcgagg cgaagagacg gaacggctgg
     7921 tcgatcgctc cagcaacgta gaccaacgtc ttgccatcgt ccgacaccgc gaactgcagc
     7981 acgcgccggt tctcggcggt gatggcttca gcctcgccct tgccgagcgg gatccggtag
     8041 atcgaggtcg caccgcgcgc cgagccgctc accactagcg tggcattgtc gatccagaca
     8101 ggcggcgtgc cgttgccagt ggcgaggtcg ctcatgcccg tgtcggagaa ggtgacgtcg
     8161 acgtccgccg tgtggttggt gagctccgat ccatcggtct tgacggtcca gacccgcgtg
     8221 taacgaccga agccggtca
//
LOCUS       DASYWL010000222         3591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_648611, whole genome shotgun sequence.
ACCESSION   DASYWL010000222 DASYWL010000000
VERSION     DASYWL010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648611"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(738..1106)
                     /locus_tag="VGR22_07815"
     CDS             complement(738..1106)
                     /locus_tag="VGR22_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128507.1"
                     /translation="MKADRPSRRKEAPSLFALREGFEHPVIDTLLGFVRRHQLEITGF
                     EFIETADGRLVVYDVNTNTNYNPEVEAVAPRSGPAAIARYLQEVARAASAPAWNGTSL
                     GTPLTRPRRRVFPKPRQSPT"
     gene            complement(1124..2266)
                     /locus_tag="VGR22_07820"
     CDS             complement(1124..2266)
                     /locus_tag="VGR22_07820"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV2128508.1"
                     /translation="MDPSAPVGETQSSPRTPHPMEVVRPTAAPTAAWWRGAAIAAIAL
                     ALAAGALVLLWLLARPLTLLLIAIVIAQALTPIVALLEQWLPRGIAIVLVYLALVLMV
                     GGIGWLVLPPLVAEGQTLVINAPELISRSREWLNGIDPESSNRVMAAVQSAIDRFADV
                     LLSLPFTLFSSVIDVILIVFISLYWLIATPALSRFTLSLFPPEHQRRANDVMDAIGET
                     MGGYVRATAINGVIIGVMTYIGLLVIGVEYTLILAVLAGLGEFLPVVGPILAAIPAIA
                     IALLESPQQAVVVAVFFIALQQLESNLLVPFIMRQQADVPPLLSLLALLAGSALGGLL
                     GALIAIPLAGAVRVLVIRVLAPAEREWAGADDPKVTDGRRATKVEG"
     gene            2679..>3591
                     /locus_tag="VGR22_07825"
     CDS             2679..>3591
                     /locus_tag="VGR22_07825"
                     /inference="COORDINATES: protein motif:HMM:NF017967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEV2128509.1"
                     /translation="MIAPLFFLLAFTARPEVASLAAQDQTMVVALGESNTPEQVVELL
                     GYFGADDDDTIVTVSLAETREVTRGIYDLSGINTAFSSSALSCRPEGTGLEVQTRNIE
                     VVVPAMYAMALLTAGIDDASLLVAAPEADPALGMTALSGVFQTWDLAPCRSVVVEERR
                     ALAVQQLALTIEIEESLGSAVGIDQVVDLVLAVQEQVLTEQMNDEDAIAHVVADQESA
                     AKLELPREERAALVRLMTGLVALDLDWGTFAAGWTLERDPGDGHVVVTGHAPRSEEVA
                     SGSPAPPRGVGGMVAGTTPAPTPSATSR"
BASE COUNT          681 a         1147 c         1163 g          600 t
ORIGIN      
        1 cggaggcatc gccgccgacg tcgaggaccg acaccgtgac ggcgacggtg aggacctgtt
       61 ccgccgtgac cgagccgacc tgaccgagcc cgagcagccg gaagcccgga ttacggagcg
      121 ccgccaggtc gatggagatc cgtccgggcc tcacgcgctc accgccccaa gcgcctcgcc
      181 tgctcaacac tgccaatgat agacgggacg ctgaccaggc cttccggcat caccagcccc
      241 cacgcctcgc cacggcatgg ggtgcggacc agggcagggt cggcgtcgcc tgaaactacc
      301 gaataggggg aacgcttggc tagcaaacgt atggtccgag tgttagttcc ttctccagct
      361 gcctgcagcc tggcgctcgg gcatcgtgtt tggtcggtga agttgtccta gtgatccgtc
      421 cgcgaaccaa gcggaccagc gatccacgaa ttcgtccaac tcgcccatga cgagacgtga
      481 gaatggccac gccacttcga attcagcgac accgggccac agggcctttg cggagtcctc
      541 gcatggtagc aacgcaaaag ggtcctgctt cccctggcgc aatgaaaaag cctgaaacat
      601 gaacgccgcc tcgtaccagc cgaactcgtc ccgcttctgc acgtcacaaa accaaagtga
      661 gtgactcctg ccctcataac cgtatcgatc cgctgacact cgaataccga tcgttgcgtg
      721 agcgacgggg tatagtgtca agtcggggat tgcctcggtt ttgggaatac tcggcgccga
      781 gggcgcgtca gcggcgtacc gagtgatgtt ccgttccacg caggcgcgct ggcggcccgg
      841 gctacctcct gcaggtaccg ggcgatcgcg gcgggacctg accttggcgc gaccgcctcg
      901 acctccgggt tgtaattcgt gttggtgttg acgtcgtaga cgaccaggcg accgtcggcg
      961 gtttcgatga actcgaaccc ggtgatctcg agctggtgcc ggcgcacgaa tccgagcagc
     1021 gtgtcgatga cgggatgctc gaacccctcg cgcagcgcga acaggctcgg cgcttccttc
     1081 cgccgtgacg gtctgtccgc tttcaacagc cccgtttctc ctctcacccc tcaaccttag
     1141 tggcacgtcg cccatccgtg accttcgggt cgtccgcgcc ggcccattcc cgttccgccg
     1201 gggcgagaac ccggatgacc aggacacgca ctgccccggc cagggggatc gcgatgaggg
     1261 cgccaagaag accgccgagc gccgagccgg cgaggagggc taacaacgac agcagtggcg
     1321 gcacgtcagc ctgctgacgc atgatgaacg ggactagcag attgctctcc aattgctgga
     1381 gcgcgatgaa aaagaccgcc accaccacgg cctgttgcgg ggactcaagc aacgcaatcg
     1441 cgatcgccgg gatcgccgca aggatcggtc cgaccactgg caagaactcg ccgagacccg
     1501 cgagcacggc caggatcagc gtgtactcca cgccgatgac gagcagtcca atgtaagtca
     1561 tcactccgat gatcaccccg ttgatggccg ttgcgcgaac atagccgccc atggtttcgc
     1621 cgatggcatc catcacgtcg ttagcccgcc gctggtgctc aggcggaaag agtgaaagcg
     1681 tgaaccggct caaggcgggt gtggcaatca gccaatacag ggatatgaaa acgatcagga
     1741 taacgtcgat gactgacgag aagagcgtga atggcagcga cagcagcaca tcggcaaacc
     1801 ggtcgatagc ggactgcacg gcagccatga cgcggttgga gctctccggg tcaatgccat
     1861 tgagccattc gcgggaccgg gagatcagct ccggcgcgtt gatcaccagc gtctggccct
     1921 cagcgacgag cggtggcaat accagccaac cgatcccccc gaccatcagc accagcgcga
     1981 ggtagacgag cacaatggca atgccacgcg gcaaccactg ctccaacagg gcgacaatcg
     2041 gcgttagcgc ctgagcgatg acaattgcga tcaggagcaa ggtcagtggc cgggccaaga
     2101 gccagagcag aaccagagcg ccggccgcca gcgccagcgc gatggcggcg atagcggcgc
     2161 cgcgccacca ggcggcggtc ggcgccgcgg tcggccgcac gacctccatc ggatgcggcg
     2221 tgcggggaga cgactgcgtc tctcctacgg gcgccgacgg atccatcaga ggcttctccc
     2281 gccgaaaaag gcattggacc tatcgagtac cacgtcgcta cccccagttc tgataagagc
     2341 gcctcccatc ggcttcgttt cggcgccgct ccggtccccg agggaagctg ctcccgtaca
     2401 ggctctaagt acctcctctg agcggtttta gcgcgatctg ctctcatgtg cgcggtgcat
     2461 gtactgtgcc attagggcgc cactcgtgtc tgcacgttac tcccctccat gtggcgttga
     2521 cagccgacaa gggtgtacgt acactgtggc gagagccgga tgtatggaac aggcgggggg
     2581 cactgcctgt ccggtgaacg ccagcagggg gcgacgcgat ggggccacgg ccggacgata
     2641 tgcgcggacc gacaatcgta cgccagtgga ggtgtctgat gattgccccg ctgttcttcc
     2701 tgctggcctt cacggccaga ccggaagtcg caagcctggc ggcgcaagat cagaccatgg
     2761 tagtggcgtt gggcgaaagt aacacacccg agcaggttgt cgagctgctc gggtacttcg
     2821 gtgctgacga cgatgacact atcgtcacgg tttcgctcgc tgagacccgt gaggtcacgc
     2881 gtgggatcta cgacctctca gggatcaata ccgcattttc ctcctccgcg ttgtcctgcc
     2941 ggccagaagg cacggggctg gaggtgcaga cccgcaatat cgaggtggtg gtccccgcga
     3001 tgtatgcgat ggcgctgctc accgcgggca ttgacgacgc ctcgctgctc gtcgcggcgc
     3061 ccgaggcgga ccccgcgtta gggatgacgg cactctcggg cgtcttccaa acctgggacc
     3121 tggcgccgtg ccgcagcgtc gtcgttgagg agcgccgcgc gctcgcggtc caacagttgg
     3181 cgctgacgat cgagattgag gagtcgctgg ggagtgcggt cgggatcgac caggtggtgg
     3241 atctggtgct tgcggtccag gagcaggtcc tgaccgagca gatgaacgac gaagatgcca
     3301 tcgcgcacgt cgtcgccgac caggagagcg cggcgaagtt ggaactcccc cgcgaagaac
     3361 gcgcggcgtt ggttcggttg atgaccggcc ttgtcgcgct tgacctcgac tggggaacgt
     3421 tcgccgccgg ctggacgttg gagcgggatc ccggggatgg tcacgtcgtg gtcaccggtc
     3481 acgccccgcg ttccgaggag gttgcttccg gatcgccagc tcctcccagg ggggttggtg
     3541 gcatggtcgc cggcaccaca cccgctccga cgccgagtgc aacgtcgcga g
//
LOCUS       DASYWL010000223        10238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_123244, whole genome shotgun sequence.
ACCESSION   DASYWL010000223 DASYWL010000000
VERSION     DASYWL010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10238
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123244"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..1078)
                     /gene="lysX"
                     /locus_tag="VGR22_07830"
     CDS             complement(164..1078)
                     /gene="lysX"
                     /locus_tag="VGR22_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysX"
                     /protein_id="HEV2128510.1"
                     /translation="MTIFGDRPPRLGILVSYLRQEEKLILAAARARGIDVIPLFDRDL
                     ALNLSAPTAAASGVDIDVLLDRSVVHSRAGYTLHAMAHWGIPTLNTAEAVTVCDDKAR
                     ASMVLEAAGIPSPKTYVAYSANSAIEACEALGYPAVLKPVTGSWGRLLAKVNGPEQAR
                     AIIAQKAEHGSFHHEIFYVQEFIEKPGRDIRAYVVGDEVVAASYRTSDHWITNAAKGA
                     VSVPCEVTPEIEALSMKACHAVGARLAGVDLIETDGGLKVIEINTGGEFKGLMTTTDR
                     DIAGVIVEETMRLARGAREGAAEPATIP"
     gene            complement(1075..2190)
                     /locus_tag="VGR22_07835"
     CDS             complement(1075..2190)
                     /locus_tag="VGR22_07835"
                     /inference="COORDINATES: protein motif:HMM:NF003367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[LysW]-lysine hydrolase"
                     /protein_id="HEV2128511.1"
                     /translation="MSLASRSLPEVDQTSPQALADIELLRELVSIESLSNHEGPAVSF
                     LRSAMASRGFRVKDSAAGSAIGTIGEGERHIVLLGHIDTVPGQIPVRIENDVLWGRGA
                     VDAKGPLATFAAAASAAAEQLQCRVTVVGATGEEVIGSPGANEISCWPAPDFCIIGEP
                     SGWDSVCLGYRGTMSLIYRLEQSTRHTAGPGESAAEHAVRFWNSVLAELDEWNAGATS
                     SFGSIGHSLRSFNTEGDGLTDVATLSIGLRLPPGIDIERLLDRIRALAGTVDIRIDGI
                     QAGYRTTKRSALIPPFLRGIRGIGGTPGFKVKLGTSDMNVVGPVWTCPIVAYGPGDSA
                     LDHTPDERIDLSEYLLAIGVLRDVLIALGRPRKEGAA"
     gene            complement(2162..3094)
                     /locus_tag="VGR22_07840"
     CDS             complement(2162..3094)
                     /locus_tag="VGR22_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128512.1"
                     /translation="MTAMGVLCARVRVEEKQIIAALADAGVVALPVPPASTPMPPGPA
                     SADLVTLGTRQHEHDGETLHHALGTMIDRCQNRHIAATLLPLLRLSGIEVIDAGLAAT
                     GSRIEVATVLVQAGIARPHAFVAMSEATALEAAARLGYPATLFPQDAGSLATALLDHD
                     TAEAVIEHRVVLGDHSEAIMLLQAGAPQEAQRSRHHVIGGEVVAVEGALVEARAQELA
                     VAAAGALGAKAITVDIALADDQVVVWDVLAVADFRQATLVGSRSVSEAFAALIVSDDR
                     VPTATTMEDVPVAMTHEGWEVQVRDGVIGISLTA"
     gene            complement(3232..3393)
                     /gene="lysW"
                     /locus_tag="VGR22_07845"
     CDS             complement(3232..3393)
                     /gene="lysW"
                     /locus_tag="VGR22_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019878 - lysine biosynthetic process via
                     aminoadipic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysW"
                     /protein_id="HEV2128513.1"
                     /translation="MATCPECGADVEVEGVEQGEIVPCPDCGADLEVLSTDPAELGLA
                     PEEGEDWGE"
     gene            complement(3454..4509)
                     /gene="argF"
                     /locus_tag="VGR22_07850"
     CDS             complement(3454..4509)
                     /gene="argF"
                     /locus_tag="VGR22_07850"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEV2128514.1"
                     /translation="MTSEDDDMVDLAEQLTHPDAVEAGQVTLLPSLMGRSILRDTDLS
                     HEEIEAVLDLAIALKDLRARHVPHAWLAAKTLGMIFQHPSTRTRTAFQAGMEQLGGQA
                     IFLGVQDLQLRRGETIEDTAGIFSRYVDAIAVRVADREDLFGLARGATVPVFNGLTSH
                     DHPIEALSDLMTLKERFGALPGLRFAYLGDGNNVCHSLMLACAATGVHFAYAGPNAWW
                     PDPEVVETAKGLAIDAGGSVTVTDDVETAVAGVDAVYTDVHESMGEQEIPGKLMALAP
                     YRVTATVMATAGEPAVFMHCLPLHRGQEVDAEVADGPQSIIFDQAENRLHVHKAVLLQ
                     VLHDERRGSDRDPASVR"
     gene            complement(4534..5625)
                     /gene="argF"
                     /locus_tag="VGR22_07855"
     CDS             complement(4534..5625)
                     /gene="argF"
                     /locus_tag="VGR22_07855"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEV2128515.1"
                     /translation="MNHQSAASVVDTTPAQVPELHSVPSTPQPPTASALKRTVVYGAQ
                     QATSLPAFTVSTRLRGRHVLLDTDLTPDEIHEVIDTASRIKRMWQNGQPHAYLAGKTL
                     GLIFQHPSTRTRVSLEAGMAQLGGQAIFLGVNDLQMKRGETIADTAQILSRYVDAIVA
                     RVADHNDLVTLAGSATIPVLNGLSDQAHPLQSYADLLTLQEAFGGLKGLKLVYLGDSN
                     NMAVSLMLSCAVTGVSVSVASPKQYQPSEDVVNHAKWLGKATGATIEITDDPAAAVKG
                     ANAVYTDVHVSMGQLDGAARAVALAPYKVTPELMGQADEKAVFMHCLPMHRDEEVSTE
                     VADGPASIIFDQAENRLHMHKALLLHTMC"
     gene            complement(6052..7332)
                     /locus_tag="VGR22_07860"
     CDS             complement(6052..7332)
                     /locus_tag="VGR22_07860"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEV2128516.1"
                     /translation="MTTFDTNGSSVPANGEKPSIGKVVLAYSGGLDTSAIIPWLKEHY
                     ECEVIAYSADVGQPDDLSGIEEKAIATGATKAHVLDLKEEFIRDYVFPTLRAGAIYER
                     KYLLGTAMARPLIAKKQVEIAKLEGAGAVAHGCTGKGNDQVRFELTYMSMAPELTIIA
                     PWRHWHIASREDAIDYCHAHGVMVEATKNKIYSLDGNLWHLSHEGGLVEDPWQEPPAD
                     AYSLTVAPEEAPDTPEHMTVSFEQGTPVAVDGQQLGPVEIVEHLNALGGKHGVGREIM
                     LENRLVGMKSRGVYEAPGGTILSAAHKELEHLTLDKQTMRLKDQLAQTYADLVYNGQW
                     FTPAKDALDAFVDKTQEVVTGDVRLKLYKGACTAVAMKSPYSLYDGDLATFGADDVYQ
                     QADAEGFIRLFGLGQKVANQRARNANLVGTEVTG"
     gene            7969..8253
                     /locus_tag="VGR22_07865"
     CDS             7969..8253
                     /locus_tag="VGR22_07865"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEV2128517.1"
                     /translation="MKCPIDQTTLQITDRQGIEIDWCPTCRGVWLDRGELDKIIERSG
                     TIAEGGRRVDDDDRRSYYDDDDREARTRYERDPRRRRKRKSFLDDLFDFD"
     gene            complement(8441..>10238)
                     /locus_tag="VGR22_07870"
     CDS             complement(8441..>10238)
                     /locus_tag="VGR22_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128518.1"
                     /translation="SHAMQPDQSTVRVSLADWLGAHSTVITRSVTAAIVLGVLVRLVA
                     ALTLSPHVDEPASVLAAHVVAERGLPLLPSGTPYFQGLTLSYLLQPFVWLGIGGIEHL
                     HLMRLVLVLAGAVTLYLCYRLALVVTGDPRVGVVMATLVALDPVSVQWSAHVRMYGLL
                     QPLTLALAWAWIVFLRGNTGWRQVALVAGIYWAAVFTHVGASLLAPAMAVAAFVLWRW
                     QVFRRWRLMVTLTIAALGPMLLLALNELFGTANEPVRESSSLPFFVGANLLAPLTEIT
                     HPVEEWTARLTAGITLYWMMPVIIVGVSTLIGARYLLRRGTGDDTRAAVAALLALYWL
                     PLLAVVLLTASRKVRYIVHLHVLGYLFLAVLLVALLHQTVTLRGDSRLRLLARHAAII
                     GIVTAVVAGLGWRLENRVVQPDYNQAMAYVADRHQPGEPVIVTLPPVGYLTMDESNRD
                     DLYFLAGSAGWTRADRYTRWDEDGRLIDYWIGADSIVSTRMLDEMLATHDNAWVIADR
                     DRLEDDWTSTTAIERVIRESMFPVYASDGGAVVYRTLPEQFRHDADILTNDPAASGVG
                     VDDACLLQMDCPPLPATPVASPAARGPSVTIW"
BASE COUNT         1760 a         3397 c         3364 g         1717 t
ORIGIN      
        1 agcatgacag gatgcagtta gcgagtgatc gaaagtcgac ttccatcgat gacgggccgg
       61 cacgaggccg gcgctacggg ttgcatcggg cgggcacctg cctcgcaggt ctgtccttgc
      121 gaggctgcgc cctccagaca acgggagggc agaaggccct cccctacggg atggtggcgg
      181 gttcggcggc tccttcccgg gcaccgcggg cgaggcgcat ggtctcctcg acgatgacac
      241 cggcgatgtc gcggtccgtc gtcgtcatca gccccttgaa ctcgccgccg gtgttgatct
      301 cgatcacctt gagcccgccg tcggtctcga tcagatcgac ccccgccagc cgcgcgccga
      361 ccgcgtggca cgccttcatc gagagcgcct cgatctccgg cgtgacctcg cacggcaccg
      421 acacggcccc cttggcggca ttggtgatcc agtggtccga ggtgcggtac gatgcagcca
      481 caacctcgtc cccgacgacg taggctcgga tatcccgccc gggtttctca atgaactcct
      541 gcacatagaa gatctcgtgg tggaaggagc cgtgctcggc cttctgagcg atgatcgccc
      601 gcgcctgctc cggcccgttc accttcgcca gcaaccggcc ccatgacccg gtcaccggtt
      661 tcagcacggc ggggtagccg agcgcctcac acgcctcaat ggctgagttc gccgaatagg
      721 ccacgtaggt cttcggcgag ggaatgcctg ccgcctccag caccatactg gcgcgcgcct
      781 tgtcatcgca gacggtcaca gcctcggcag tgttcagcgt ggggatgccc cagtgtgcca
      841 tcgcatgcag tgtgtagccc gcgcgggaat ggaccacgct gcggtcgagc agtacgtcga
      901 tgtcgacacc gctcgcggcg gcggtcggcg cggagaggtt gagtgccagg tcgcggtcga
      961 agagcgggat aacgtcgatg ccgcgcgccc gcgccgccgc gaggatgagt ttctcctcct
     1021 gtcgcaggta ggagacgaga atgccgagcc gtggcggtcg gtcaccgaag atggtcatgc
     1081 ggcgccctcc ttccggggcc gtccgagtgc gatgaggaca tcccgcaaca cgccgatcgc
     1141 caacaggtac tcggacaggt cgatccgctc gtccggcgtg tggtccagcg cggagtcccc
     1201 cggcccgtag gcaacgatcg ggcacgtcca gaccggcccg acgacgttca tgtcggacgt
     1261 gccgagcttc accttgaacc ccggcgtgcc gccgatgccg cgaatgccac gcaggaacgg
     1321 tgggatcagg gccgaccgct tggtggtgcg gtagcccgcc tggatgccat cgatgcggat
     1381 gtcgaccgtg ccagcgagcg cgcggatgcg gtcgagcagc cgctcgatgt cgatgcccgg
     1441 cggcagccgc agcccgatgg agagggttgc gacgtcggtg agaccatcgc cctcggtatt
     1501 gaacgaccgc agggagtggc cgatggagcc gaagctggag gttgcgccgg cgttccactc
     1561 gtccagctcg gccagcacgc tgttccagaa ccggacggcg tgctcggcgg ccgactcgcc
     1621 cggccctgcc gtgtgccggg tcgactgctc cagccgatag atgagcgaca ttgtgccccg
     1681 gtagccgagg cagacgctgt cccacccgct gggctcgccg atgatgcaga aatcgggagc
     1741 cggccagcag gagatctcat tggcaccggg cgagccaatc acctcctcgc cggtcgcgcc
     1801 aacgacggtg acgcggcact gaagctgctc cgctgccgcc gacgcggcgg cggcgaaggt
     1861 ggccagcggc cccttggcgt caaccgcacc gcgcccccac aggacgtcgt tctcgatgcg
     1921 aaccggaatc tgccccggca cggtatcgat gtggcccagc aggacgatgt ggcgctcgcc
     1981 ctcgccaatg gtgccgatcg cgctgccagc ggcggagtct ttcacgcgga agccgcggga
     2041 cgccatggcc gagcgcagga aggataccgc cggtccctca tggttcgaga ggctctcgat
     2101 ggagaccagc tcgcgaagca gttcgatatc ggcaagcgcc tgcggggagg tctggtcgac
     2161 ctcaggcagt gagcgagatg ccaatgacac catctctcac ctgcacttcc catccctcat
     2221 gcgtcatcgc gaccgggaca tcctccatcg tcgttgcggt cgggacacga tcatcggaaa
     2281 caatgagcgc ggcgaacgcc tcgctgacgc tgcggctgcc gactagcgtc gcctgtcgga
     2341 aatcagcaac tgccagcaca tcccagacga ccacctggtc atcggccagt gcgatgtcga
     2401 ccgtgatcgc ttttgcgccg agggcaccag ccgcggcgac cgccaactcc tgggcgcggg
     2461 cctccacgag cgcaccctcg acggcgacga cctcgccgcc gatgacgtga tggcgactgc
     2521 gctgcgcttc ctggggcgca ccggcctgga gcagcataat cgcctcgctg tggtcgccga
     2581 gcaccacgcg atgctcgatc accgcctcgg cggtgtcgtg gtccaacagg gcagtcgcaa
     2641 ggctgccggc atcctgcggg aacagggttg ccgggtagcc gagccgagcc gccgcctcga
     2701 gggcggtggc ctccgacatc gccacaaacg cgtggggccg ggcaatgccg gcctgcacca
     2761 gcacggtcgc cacctcgatg cgcgagccgg tcgccgcgag acccgcgtcg atcacctcaa
     2821 tgccgctcag tctgagcagc ggcagcagcg tggcggcgat gtggcggttc tggcaccggt
     2881 cgatcatcgt accgagcgcg tggtgcaagg tctcgccgtc atgctcatgc tggcgggtgc
     2941 ccagcgtcac caggtcggcg gacgccgggc cgggaggcat gggggtgctc gcaggaggga
     3001 cgggcagcgc cacgacgccg gcgtcggcca gcgcggcgat gatctgcttc tcttccacgc
     3061 gcacgcgtgc acacagcaca cccattgctg tcacgttgat accccttctg ccttccgttc
     3121 agctgcggca atcaatagga gatcgcccgt acgggtcgac tccatgtcga ccctgttgca
     3181 gacggacaac tccttcggag gtccgcccct acggcaggcg cgcaagcgcg cctactcgcc
     3241 ccagtcctcg ccctcttccg gtgcgagccc aagctccgcc gggtcagtgc tcagcacctc
     3301 aagatcggcg ccgcaatccg ggcacggcac gatctcgccc tgctcaacac cctcgacctc
     3361 gacgtctgct ccacactccg gacacgttgc catagctgag tccctcctgg gccgggcctg
     3421 ctgccctgcc ctcatatcaa accgttctgt ccttcacctg acgctcgccg ggtccctgtc
     3481 cgacccgcgg cgctcgtcat gcagcacctg cagcagaacc gccttgtgca cgtgcagccg
     3541 gttctctgcc tgatcgaaga tgatcgactg gggaccatcc gcgacctcgg catcgacctc
     3601 ctgcccgcgg tgcagcggca ggcagtgcat gaagaccgcc ggctcaccgg ccgtcgccat
     3661 cactgtcgcc gtcacccggt agggtgccag cgccatcagc tttcctggaa tctcctgctc
     3721 tcccatgctc tcatggacat cggtgtagac ggcatccacc ccggccaccg ccgtctcgac
     3781 gtcgtccgtg accgtcaccg aaccgccagc atcgatcgcc agccccttgg ccgtctcgac
     3841 tacctccggg tccggccacc aggcattcgg tcccgcgtac gcaaaatgca caccggtcgc
     3901 cgcacaagcc agcatcaggg agtggcagac gttgttgccg tcgccgaggt aggcgaaccg
     3961 cagtcccggc agcgcaccga agcgctcctt gagggtcatc aggtcgctca gtgcctcgat
     4021 cggatggtca tggctcgtca ggccattgaa caccggcacc gtggcccctc gtgcgagccc
     4081 gaagagatcc tcacggtcgg caacgcggac cgcaatcgcg tcgacgtagc ggctgaagat
     4141 cccggcggta tcctcgatcg tctcgccccg cctgagctgg agatcctgca cgccgaggaa
     4201 gatcgcctgg ccgccgagct gctccatgcc agcctgaaag gcggtacgag tgcgcgtgct
     4261 tggatgctga aagatcatgc cgagcgtctt ggcggccagc cacgcatgcg ggacgtgccg
     4321 tgcgcggaga tccttgagcg cgatcgcgag gtcgaggacc gcttcgatct cctcgtggct
     4381 caggtcggta tcgcgcagga tgctgcggcc catcagggac ggcaacaggg tgacctggcc
     4441 cgcctcgacg gcatcgggat gtgtcagttg ttcagccaga tcgaccatgt cgtcatcctc
     4501 gcttgtcacc gccccatcgc tcctggcacg gcgtcagcac atcgtgtgca gcaacagcgc
     4561 cttgtgcatg tgcagccggt tctccgcctg atcgaagatg atcgacgccg gcccatcggc
     4621 cacctcggtc gaaacctcct cgtcgcggtg catcggcagg cagtgcatga agaccgcttt
     4681 ctcgtccgcc tgccccatca gctctggggt gaccttgtag ggcgccagcg cgaccgctcg
     4741 ggcggcacca tcgagctgcc ccatgctgac atgaacatca gtgtagacgg cgttggcgcc
     4801 cttgaccgct gcggcgggat catcagttat ctcgatcgtc gcgccggtcg ccttgccaag
     4861 ccacttcgca tggttgacca catcctcgct cggctggtac tgcttcggtg aggcaacact
     4921 aacactcacg cccgtcacgg cgcaggaaag catcagcgag acggccatgt tgttgctgtc
     4981 tccgaggtag accagcttca gtcccttcag cccgccaaag gcctcctgca gcgtgagcag
     5041 atcggcgtag gactgcagcg ggtgcgcctg gtcggagaga ccgttgagca ccgggatggt
     5101 cgccgagccg gccagcgtga cgagatcgtt gtggtcggcc acgcgagcga cgatcgcgtc
     5161 gacgtagcgg ctgaggatct gggcggtgtc ggcaatagtc tcgccacgct tcatctggag
     5221 atcgttgacc ccgaggaaga tcgcctggcc gccgagctgc gccatgccgg cctccagcga
     5281 aacccgcgtg cgagtgctcg ggtgctggaa gatgagcccg agggtcttgc cggcgaggta
     5341 ggcgtgcggc tggccgttct gccacatccg cttgatgcgg ctggcagtgt cgatcacctc
     5401 gtggatctcg tcaggcgtga ggtcggtgtc aagcagcaca tgccggcccc gcagccgcgt
     5461 gctgacggtg aaggccggca ggctggtagc ttgctgtgca ccgtagacga cggtgcgctt
     5521 gagcgccgac gcggtcggtg gctgcggggt cgagggcacg gagtggagct ccggcacctg
     5581 ggcgggggtt gtgtcgacaa cggacgcggc ggactggtgg ttcacgggac tcccctttcg
     5641 tcgttcgatc gttcgaagac gccggttccg atcgctcgtc gatcgcgaac cgaaggggcg
     5701 agaggcgtca catcccgcgg taccaccctc cttggagcgc cagcgccatg cgacgcgggc
     5761 tccttctctc ggctgttgca tccatgggag ccacccatcc gagcctactt gcgggaacat
     5821 cccactttct tcggccggcc actcgggggc gcgttcggca tcctggttcc gggctcccac
     5881 catccccgga tcgctcatga acggatgcgt actgatcccc gtcaatgcgg gctgcccgtg
     5941 cgcgacggac agatgctgtt tatcgctgat tcgtcctgtg gcggcgcgtc ggccgccgtt
     6001 cgggagagcg ggagagcaac tcccactcga tgccgtggtg gaggtgcgtc atcacccggt
     6061 cacctccgtg ccgaccaggt tggcgttgcg ggcccgctgg ttggcgacct tctggccgag
     6121 gccgaagagc cggatgaagc cctcggcgtc cgcctgctgg taaacgtcgt cggcgccgaa
     6181 cgtcgccaga tccccatcgt agagactgta cggtgacttc atcgcgaccg ccgtgcaggc
     6241 gcccttgtag agcttgagcc gaacatcgcc ggtgacgacc tcctgcgtct tgtcaacgaa
     6301 ggcgtcaagg gcatccttcg ccggcgtgaa ccactggccg ttgtaaacga gatcggcgta
     6361 ggtctgcgcg agctgatcct tgagccgcat ggtctgcttg tcgagagtca ggtgctcgag
     6421 ctccttgtgc gcagcggaca gaatggtgcc gcccggtgcc tcgtagacgc cccggctctt
     6481 catgccgacg agccggttct cgagcatgat ctcccggccg acgccgtgct tgccgccgag
     6541 cgcgttgagg tgctcgacga tctcgaccgg gccgagctgc tggccatcga cggcgaccgg
     6601 cgtgccctgc tcgaaggaga cagtcatgtg ctccggcgtg tccggcgcct cctccggtgc
     6661 gaccgtgagc gagtaggcat cggccggcgg ctcctgccag ggatcctcga cgaggccccc
     6721 ctcgtgcgag aggtgccaga gattgccgtc gagcgagtag atcttgttct tcgtggcctc
     6781 gaccatgacg ccgtgtgcat ggcagtagtc gatcgcgtcc tcccgcgatg cgatgtgcca
     6841 gtgtcgccac ggggcgatga tcgtgagctc gggagccatc gacatgtagg tcaactcgaa
     6901 gcgaacctgg tcgttgccct tgccggtgca accatgcgcg acggcgcccg cgccctcgag
     6961 cttcgcgatc tcgacctgct tcttcgcaat cagcggccgc gccatggcag tgccgagcag
     7021 gtacttccgc tcgtagatcg cgccagcgcg cagggtcggg aagacgtagt cgcggatgaa
     7081 ctcttccttg aggtcgagga catgggcctt ggtggcgccg gtggcgatag ccttctcctc
     7141 gatgccggag aggtcatcgg gctggccgac atcggccgag taggcaatca cctcgcactc
     7201 gtagtgctcc ttgagccaag gaatgatggc cgaggtgtcg aggccgccgg aataggcgag
     7261 cacgaccttg ccaatggagg gcttctcgcc gttggcaggc acggatgagc cgttggtgtc
     7321 gaacgtggtc atgaggatgg tgctccgtct tggtgatgcg ctgcgtcgtc aggtacaggg
     7381 gtgagcgctc gccggaccgg cgaacagaat tacctgcgac gacgggggag acgccctgcg
     7441 ggggaagcag gtgcgaatcg gttgcgcatc gggagcggtc gcatgatggc gaacatcctc
     7501 tgagtgtgcg ggccaccaaa agcgggcggt ggcaaagatg ataaacagta atcaggcgtc
     7561 cagaggacac cccacctcgc gcccgtgacg tttctcacaa gcacatgcac agcgtaacgg
     7621 ctcgctgtcg gggtgtcaac accctgcaca aaaggatggt gcacatccca cgatgtgagt
     7681 tgctgcggac cgctcctgaa ggctggctca tcgcgggcct gctcatcgct ccaattggcc
     7741 agttggttgg gccgactccc tcccggtaaa gcgcaacgcg gcagaatctg ggcatgacgc
     7801 ttgcacacat gctgttcagt gccaccgggg acgcgaggtt ggccctgcca ctgggtcacg
     7861 catgcctcgt gatgtccaat gtggtgggct gactcatctt gctgcttcag ggcatacgtc
     7921 acagcagatc tcgagcggca cgattcgcgg gcagaagaag gatccgccat gaaatgtccg
     7981 attgaccaga caaccctcca gattaccgat cgacagggca tcgagatcga ctggtgcccc
     8041 acctgccgtg gtgtatggct tgaccgtggc gagctcgaca agatcatcga gcgctccgga
     8101 acgatcgccg agggcggtcg gcgcgtcgat gatgacgacc gcaggtccta ctacgacgac
     8161 gatgatcgag aggcccggac gcggtacgag cgcgatcccc gccgtcgcag gaagcggaaa
     8221 tcgtttctcg acgacctgtt cgatttcgac taggagccgg tacccaaggg gctgcggtgc
     8281 gagcatggtt acccgttgtg ctcgccgtca cagttctcca tatgcggttg cggtgatgta
     8341 aggtccggat ggcgcgtggc gcatggttga cccttcggtg ccagcctcac catgggtgcg
     8401 ccccaaaggg ctatccggct gcgtcaccaa cgccggatca tcaccagatg gtgaccgacg
     8461 gcccacgagc ggcgggggac gccaccggcg tagcgggcag cggcgggcag tccatctgga
     8521 gcaggcatgc gtcatcgaca cccacaccgc tcgcggcggg atcgttcgtg aggatgtccg
     8581 cgtcgtgcct gaactgctcc ggcagtgttc ggtagacgac cgcaccgcca tcgctggcgt
     8641 agacggggaa catcgactcg cggatcaccc gctcgatcgc ggttgtcgag gtccagtcgt
     8701 cttcgagccg gtcgcgatca gcgatcaccc acgcgttgtc gtgggtggcg agcatctcgt
     8761 cgagcatacg ggtcgagacg atcgagtccg cgccgatcca gtagtcgatc agacgaccat
     8821 cctcgtccca ccgtgtgtag cggtcggcac gggtccagcc cgccgagccg gcaaggaagt
     8881 agagatcgtc gcggttggat tcgtccatgg tgaggtagcc gaccggtggc agcgtgacga
     8941 ttactggctc cccgggctgg tgacggtcgg cgacgtaggc catcgcctgg ttgtaatcgg
     9001 gctggaccac tcggttctcc agccgccagc caagaccggc gacgaccgcg gtgacaatgc
     9061 cgatgatcgc ggcgtgccga gccagcagac gcaaccggga atcgccacgc aacgtcactg
     9121 tctggtgcaa cagcgccacc agcagcacgg cgagaaacag atagcccagc acgtgcagat
     9181 ggacgatgta gcgcaccttc cgcgaagcag tcaacagcac cacggcgagg agtggcagcc
     9241 agtagagagc cagcagcgca gccacggcag cgcgcgtgtc atcacctgtc cctcggcgta
     9301 gcaggtaacg tgcgccgatc agcgtcgaca cgcccacgat gatgaccggc atcatccaat
     9361 agagggtgat cccggcggtc aggcgtgcgg tccactcctc cacaggatgg gtaatctcgg
     9421 tcaggggtgc cagcaggttg gcgccgacga agaagggcaa cgacgacgac tcccgcactg
     9481 gttcgttggc cgttccgaac agctcgttca gcgccagcag cagcattgga cccagcgcgg
     9541 cgatggtcag cgtgaccatc agacgccatc gtcggaacac ctgccagcgc cacaggacga
     9601 aggcagcgac cgccatcgcc ggggcgagca ggctggcacc gacgtgcgtg aagactgccg
     9661 cccagtagat ccccgccacc agagccacct gcctccagcc ggtgttgccg cgaaggaaga
     9721 cgatccacgc ccatgccagc gccagcgtga ggggttgcaa cagcccgtac atgcgaacgt
     9781 gtgcgctcca ctggacgctc accggatcga gcgcgaccag tgtcgccatc accacgccga
     9841 cgcgcggatc accggtgacg accagcgcca ggcggtaaca gagatagagc gtgacggctc
     9901 cggccagcac cagcaccaac cgcatcaggt gcagatgctc aatcccaccg atgccgagcc
     9961 agacgaaggg ctgcagcagg tatgagaggg tcaaaccctg gaaataggga gtaccggagg
    10021 gcagcaatgg cagcccacgc tcggcgacca cgtgggcggc gagcacgctg gccggctcgt
    10081 cgacgtgcgg cgagagcgtc aacgcggcca ccagccgaac cagcacgccg agcacaatcg
    10141 ctgcagtgac gctccgcgtg atgacggtgc tgtgcgcgcc aagccagtca gccagactga
    10201 ccctgacggt cgattggtca ggctgcattg catgagaa
//
LOCUS       DASYWL010000224         7136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_348339, whole genome shotgun sequence.
ACCESSION   DASYWL010000224 DASYWL010000000
VERSION     DASYWL010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7136
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348339"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..109
                     /locus_tag="VGR22_07875"
     CDS             2..109
                     /locus_tag="VGR22_07875"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEV2128519.1"
                     /translation="MVSALAGRDQIMRAYREAIVERYRFFSFGDAMLII"
     gene            140..724
                     /locus_tag="VGR22_07880"
     CDS             140..724
                     /locus_tag="VGR22_07880"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV2128520.1"
                     /translation="MAYPDLAGKTRVDGLALLDEWIESGSLKRHCLSVETAMRAYAEK
                     WGEAVEAWGMVGLIHDFDFEKHPTLDSHPVEGAPVLRERGYPEWVVDAILSHGENTGV
                     ARDTPLRKTLYAVDELSGFVSAVALVRPSKSVRDVKVSSVKKKLKDKRFAEAVDRDQI
                     RESAKELGVDFDEHIATLIEALSNNAEELGLAGR"
     gene            818..1342
                     /locus_tag="VGR22_07885"
     CDS             818..1342
                     /locus_tag="VGR22_07885"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV2128521.1"
                     /translation="MQQWSQPFENQLKEDASYTADLETNFGTITAEFYPEDAPKTVNN
                     FVRLAREGFYDGTVFHRVVKGFVIQGGDPTGTGAGGPGYKFEDEPIVKDYEPGTLAMA
                     NAGPNTNGSQFFIVLEDMRGRLPKNYTIFGKVTDGMDVVKKIGDVPVQPSPSGEPSSP
                     VERIELTSVKVNES"
     gene            complement(1418..1642)
                     /locus_tag="VGR22_07890"
     CDS             complement(1418..1642)
                     /locus_tag="VGR22_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128522.1"
                     /translation="MAGSTAHPTTSRLLRELDVLEHRLRDGEHRIEEARDAGADVSAW
                     EEFWIDLLRTYEATHDRLAAMVATDSRAAA"
     gene            complement(1668..2279)
                     /locus_tag="VGR22_07895"
     CDS             complement(1668..2279)
                     /locus_tag="VGR22_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128523.1"
                     /translation="MFNPQDHVTIVDGAAYLEVKWRLVWLREQHPDATIETDMVESSR
                     DHAIFKAFIRLPNGASSTGWGSEYREQFFNFIEAAETKAIGRALAALGFGTQFAGDYS
                     SDSGARAGLSDAPVRLRAVEDGDRRSQPQGQQAGELEPTERQRKLIDVLRSQLNITVD
                     QLDELAMEHGGAPMESIGRKGVSALITELQRIKQEKQERAKAS"
     gene            2488..3630
                     /gene="dnaN"
                     /locus_tag="VGR22_07900"
     CDS             2488..3630
                     /gene="dnaN"
                     /locus_tag="VGR22_07900"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEV2128524.1"
                     /translation="MKVSCLQENLQRALGRVSRAVATRTTLPVLSNVWIGTEDGRLKI
                     AATNMEIGVTTWIDASVEDEGQITVDARLLSEFVNTLPTGEVKLDLDPNRLSLTVRAG
                     RDKAAINGIDPEDFPVITTMKEGNFTISIDAQTLRDRISLVEFAAATDESRPVLAGVL
                     TRFDGDNMMLAAADGFRMAVSEGPSGVSVPERQDVIVPARSYRELARLVADYDEEITI
                     SVAGNKAQIIARFGDTEWVSQLIDGTFPDVKQIVPREFATRVDVGRDTLLNAVRRASY
                     FARENNDVIRLAIHAGEDDMTPGSVEVTANAAERGNSESFVDASIAGGELQIAFNSRY
                     LSDVLGVLKHGNIMLGLNGANQAGVVRPSGAENYSHVIMPMVIGGQ"
     gene            3642..4832
                     /locus_tag="VGR22_07905"
     CDS             3642..4832
                     /locus_tag="VGR22_07905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEV2128525.1"
                     /translation="MHLETISIAQFRCHAGVRITVPDAGVRIVGPNGSGKTSILEAIG
                     MLAITRSFRGANDRDVVSWGSGKDFGVAPFSRIEATVETDGEHRRLGIALELADGGET
                     VQSKRFFVDDKLTTAHGLVGNLRTVTFTPEDVQLVIGPPADRRRPLDVLISQLDRRYM
                     RRLAAYGRVLRQRNGLLKAFARDRVRPEATAAVMQLAFWDEQLVAEGGYLIARRAQVV
                     DKLDAAIRRRASSMSNGNDFSLQYDPRVRMQAESLTGDLSLDDAAVRANEAFRRDLEA
                     HREDEFRRGMTLTGPHRDDVLYLNRGRSLARFGSRGQQRLAVLALKLAEADVISMESG
                     DTPVFLLDDILSELDPRHRSMLLDELANRQAQTILTSADDEVLDHPALEALGRFQTVV
                     PTER"
     gene            complement(5688..6662)
                     /locus_tag="VGR22_07910"
     CDS             complement(5688..6662)
                     /locus_tag="VGR22_07910"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2128526.1"
                     /translation="METTPGETGAGTDLRVRPLRTDDLAAVDTVRRLAFGTFLGLPDP
                     LTFRGDANPVRTRYLADPAGSVAGDLGGEVVGTKFVVDSGSVGFFGPLSVRPDLWDRG
                     IARRLVEAALAEFDRRGTRHVGLFTFAHSPKHLALYQRYGFWPRFLTAIMGKPVRQPD
                     IEVRWRAYADVPSEEQPAYLEKCREVSDAVHPGLDLSREIRATADHGFGDIVLLPDGR
                     DLVGFAVCHCGADTEAGRGSCYVKFGAVRSGPDAEQWFLRLLAACEALAVSWGQQRLT
                     AGVNTARREAYRRMQGLGFRTEVLGLAMDRPDEPGYNRPGVYLIDDWR"
     gene            complement(6941..>7136)
                     /locus_tag="VGR22_07915"
     CDS             complement(6941..>7136)
                     /locus_tag="VGR22_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128527.1"
                     /translation="FSVLSSALTEMVNGPLRLGPLFAFVVASSELSLGGLGSAFWALV
                     IGMTVTLVLEPNEWRAVRSP"
BASE COUNT         1283 a         2273 c         2338 g         1242 t
ORIGIN      
        1 gatggtgagc gcgcttgcgg gacgcgacca gatcatgcga gcgtatcgag aggcgatcgt
       61 cgagcggtac cggttctttt cgttcggcga cgccatgctg attatttgat cgaatagcac
      121 aggggagcgt tgcgtaccga tggcgtatcc ggatctggca ggaaagacgc gcgtggacgg
      181 cctcgcgctg ctggacgagt ggattgagag cggcagcctg aagcgtcact gtctctcggt
      241 ggagacggcc atgcgcgcct acgcggagaa gtggggcgag gctgtcgagg cctgggggat
      301 ggtcgggctc atccatgact tcgacttcga gaagcacccg acgctcgaca gtcaccccgt
      361 cgagggtgcg ccggtgcttc gcgagcgtgg ctacccggag tgggtggtcg acgcaatcct
      421 gagccacgga gagaacacgg gcgttgcgcg ggacactccg ttgcgcaaga cactctacgc
      481 ggtggacgag ctctccgggt tcgtttcggc ggtcgcgctg gttcgcccgt ccaagtccgt
      541 gcgcgatgtg aaggtctcgt cggtcaagaa gaagctgaag gacaagcgct ttgccgaggc
      601 ggtcgaccga gaccaaatcc gagagagcgc caaggagctg ggcgtcgact tcgacgagca
      661 catcgcgacc ctcatcgagg cgctctcgaa caatgcggag gagcttggtc tggccggcag
      721 gtaacggcgc tacgccatgc ccacgaggga cccagtgtaa actcttgacc gaagatacgg
      781 cagactggct atcgatcaaa ccgagaggga ctataccgtg cagcagtgga gccagccatt
      841 cgaaaaccag ctgaaggagg atgcgtccta caccgctgat ctcgagacca acttcggcac
      901 catcaccgcc gagttctatc ccgaagatgc tccgaagacg gtcaacaact ttgtcagact
      961 tgcgcgggag ggcttctacg acggcaccgt cttccatcgc gtcgtcaagg gcttcgtcat
     1021 ccagggtggc gacccgaccg gtacaggcgc tggcgggccc ggctacaaat tcgaggacga
     1081 gccgattgtc aaggactacg agccgggcac acttgcgatg gcgaatgcgg gtccgaacac
     1141 caacggcagc cagttcttca tcgtgctgga ggacatgcgg ggccgcctgc cgaagaacta
     1201 caccatcttc ggcaaggtga cggatggcat ggacgtcgtg aagaagatcg gcgacgtgcc
     1261 ggtgcagccg agcccgagcg gcgagccctc gtcgcctgtc gagaggatcg agctcacgtc
     1321 agtcaaggtc aacgagagct agcttttcgg acgttgagca cggcgaaatg ccctcccgat
     1381 tcatcgggag ggcatttcgc gtcgggacgg ttgcccgtca ggcggcggcg cggctgtccg
     1441 tggcgaccat cgccgcgaga cgatcgtgag tcgcctcgta ggtgcgcagg agatcgatcc
     1501 agaactcctc ccacgccgag acgtcggcgc cggcgtctcg cgcctcctcg atacggtgct
     1561 cgccatcgcg aagccgatgc tcgaggacgt cgagttcgcg cagcaggcgc gaggtggtgg
     1621 gatgggccgt tgatcctgcc attggttctg ctcccgcggg acgcgcgcta gctcgccttc
     1681 gcgcgctcct gcttctcctg cttgatgcgc tgcagctcgg tgatgagggc cgagacaccc
     1741 ttgcggccga tcgactccat tggcgcaccc ccgtgctcca tggcaagctc gtcgagctgg
     1801 tcgactgtga tgttgagctg gctgcgaagc acgtcgatca gcttccgctg ccgctctgtc
     1861 ggctcgagct cgccggcctg ctggccctgc ggctgcgacc tgcgatcgcc gtcctcgacg
     1921 gcacgaagcc gaaccggggc gtccgagagg cccgctcggg ctccgctgtc gctcgagtaa
     1981 tctccagcga actgagtgcc gaaaccgagc gccgccagcg cgcggccgat cgccttggtc
     2041 tccgctgcct caatgaagtt gaagaactgc tcgcggtact cgctgcccca tccagtcgaa
     2101 gaagcaccgt tcggcagccg gataaacgcc ttgaagatcg cgtggtcgcg cgaggattcg
     2161 accatgtcag tttcgatcgt ggcatccgga tgctgctcgc gaagccatac gagacgccac
     2221 ttcacttcga gataggcggc gccgtcgaca atcgtcacgt ggtcctgcgg gttaaacact
     2281 cgagcctcct gtgcttctgg ttctggttta tcagaacgaa tgttctacgg accatactag
     2341 aacatttggt ctgtcttgtc aagcaggcac agaagggtgt gggcagagtc gtcggcgccg
     2401 gcgtccgaat ggcgacggtg tgtccgtatg ctatgattcc agccgaaaaa ccgtcgtcga
     2461 tcgtcagtca gtaagcgagg aatcccggtg aaggtatcgt gtctccagga aaatctgcag
     2521 cgagcgctcg ggcgggtttc tcgagccgtt gccacgagaa cgaccttgcc ggtgttgagc
     2581 aatgtctgga tcggcacaga ggatggccgg ctgaagatcg ccgcgaccaa catggagatc
     2641 ggcgtcacga cctggatcga cgcctcggtt gaggatgagg gccaaatcac tgtcgacgcc
     2701 cgtctgcttt ccgagttcgt caacacgctg ccaaccggag aggtgaagct ggacctcgac
     2761 cccaaccgcc tgtccctcac ggttcgcgct ggtcgcgaca aggcggcaat caacggtatc
     2821 gatccggaag acttcccggt catcaccacg atgaaggaag ggaatttcac gatctcgatc
     2881 gatgcccaaa cgctgcgtga tcgcatctca ctggtcgagt tcgctgctgc gaccgacgag
     2941 agccggccgg tgctcgccgg cgtcctgacg cgcttcgacg gcgataacat gatgctcgcc
     3001 gccgccgacg gcttccgcat ggcggtctcc gaggggccgt ccggggtctc ggtccccgag
     3061 cgccaggacg tgatcgtgcc ggcccgatcc taccgcgaac ttgctcgcct cgtcgccgat
     3121 tatgatgagg agatcaccat ctcggtcgct gggaacaagg cacagatcat cgcgcgcttc
     3181 ggtgacacgg agtgggtttc ccagctgatc gacggcactt tccccgacgt aaagcagatc
     3241 gtaccgcgcg agttcgcaac ccgggtcgat gtcggtcgcg acacgctgct caacgcggtt
     3301 cggcgagcca gctacttcgc ccgtgagaac aacgacgtga tccgcctggc tatccacgct
     3361 ggcgaggatg acatgacgcc gggcagtgtc gaggtcaccg ccaacgccgc cgagcgcggg
     3421 aacagcgaga gttttgtcga cgcgagcatc gccggcggcg agcttcagat cgcgttcaac
     3481 agcaggtacc tctcggacgt gctcggcgtg ctcaagcacg gaaacatcat gctggggctg
     3541 aacggcgcga accaggcggg cgtcgtgcgg ccgagcggcg ccgagaacta cagtcacgtg
     3601 atcatgccaa tggtcatcgg cggccagtag cccgggcgcg catgcacctc gagacgattt
     3661 ccatcgccca gtttcggtgc cacgccggcg ttcggatcac ggttcccgac gccggcgttc
     3721 gcatcgttgg tccaaatggc tcgggcaaga ccagtatcct cgaggcgatc gggatgctgg
     3781 ccatcacgcg ttccttcagg ggggccaacg accgcgacgt cgtgagttgg gggagcggga
     3841 aagacttcgg tgtcgcgccg ttctcgcgca ttgaggcgac ggtcgaaacc gatggcgagc
     3901 accggaggct cggtatcgcc ttggagctcg cggacggcgg cgaaaccgtg cagtctaagc
     3961 gcttcttcgt cgacgacaag ttgacgaccg cgcacggtct cgtcggcaac ctgcgaaccg
     4021 tcaccttcac ccctgaagac gtacagctcg tgatcgggcc gccagccgac aggcggcggc
     4081 ctctcgacgt gctgatctcg cagctcgatc gcaggtatat gcggcgtctg gcggcatatg
     4141 gccgtgtact caggcagcgg aatggcttgc tgaaggcctt tgcgcgagac agggtccgac
     4201 cggaggcaac ggcggcggtg atgcagcttg ccttctggga cgagcagctg gttgccgaag
     4261 gtgggtacct gatcgccagg cgcgcgcagg tggtcgataa gctcgatgcg gcaatcaggc
     4321 ggcgcgcgag cagcatgtcg aacggcaacg acttcagctt gcagtacgac ccgcgggtac
     4381 gcatgcaggc agagtcgttg actggcgacc tatcgctcga tgacgctgcg gtacgcgcta
     4441 acgaggcctt ccgccgggat ctggaggcgc accgggagga cgagttccgg cgcggcatga
     4501 ccctcacggg gcctcatcgc gatgacgtcc tctacctcaa ccgggggcga tcgctggcgc
     4561 ggttcgggtc ccgagggcag cagcggttgg ccgtgctggc gctcaagctg gccgaggccg
     4621 atgtcatctc gatggagtcg ggtgatacgc cggtcttcct gctcgacgat atcctttcgg
     4681 agcttgaccc gcgccaccgg tcgatgctct tggacgagtt ggctaaccgg caggcgcaga
     4741 cgatcctgac cagcgctgac gacgaggtgc tcgatcatcc agcgctcgag gcactgggac
     4801 gcttccagac cgtcgtcccg acggagcggt agggtcgagt gagctgcaag acaacaggaa
     4861 gggcggccgc attgcgccgc cgcccttcct gttgtcgatg gctggggcgc agggattcga
     4921 accccaacta cctgatccag agtccgattc tctgagtctt gcaacgtctt gccgcgtccc
     4981 tttatgccgg aaaaatcggc acattcgccg ccctcgactt tcacccgatc ttgccgattc
     5041 tcgcccgctt ggctgtcaac ttggctgtca gcctccggcg tcagtcagcc ttccgggatg
     5101 gcagtagatt ccgtcagcgc ccctcggcag cgagaggctt ctgacaccat cggttcttgc
     5161 tcacgacgca cagcgggcgt atgctgcagg gatacaagga acatggcgcc gatgcatctt
     5221 ggtcgcctcg gttcccccgg tgtgcggtga ccgagcccct tggtggctcc agccggaagg
     5281 ggagaagcat ggccgcgatc tgtaagaatt gcggggggag catcttttgg gtgtccaccg
     5341 gaaccggccg gcggctggcg ctcgatgtgc atcccttgcc gcggcacgcc cgcaacggat
     5401 gggtacttga cgccggcgtc gctcgcctgg cgaacccgtc ccgtgaccat tccactatgc
     5461 ttcgctaccg aaaccattgg gcgacctgcg cccacgaccg accacgccag agatcatgat
     5521 gcggcgggct gagattagca ggccacaaat cgctccctgc gaagacatca tagccgccat
     5581 cgcgccgcag ccaggagacg ccaccgggga cgatcgccag atgtggtggg ccgcatcgcc
     5641 catcgggtag cctcgacacg gttcacgagg ctggccgcca acgggcgtta tcgccagtcg
     5701 tcgatgagat agacgccggg ccggttgtac ccgggttcgt ccggccggtc catcgccagt
     5761 cccagcacct cggtgcggaa cccgagtccc tgcatgcggc gataggcctc gcggcgggcg
     5821 gtgttgaccc cagccgtcag ccgctgctgt ccccaggaca cggccagcgc ctcacacgct
     5881 gccagtagcc ggagaaacca ctgctcggca tccgggcccg accgcaccgc cccgaacttg
     5941 acgtagcagc tgcctctccc ggcctcggtg tcagcgccgc agtgacagac cgcgaagccg
     6001 acgagatccc tgccgtcagg gagcagcacg atatcgccga agccatggtc tgcggtggcc
     6061 ctgatctcgc gactgagatc gagacccggg tgcacggcgt cggaaacctc ccggcacttc
     6121 tccaggtacg ccggttgctc ctcgcttggg acgtccgcat aggcccgcca ccggacctca
     6181 atgtccggct gacggaccgg tttgcccatg atggccgtca ggaaccgggg ccagaatccg
     6241 taccgctggt agagcgcgag gtgcttgggg ctgtgggcga aggtgaacag cccgacgtgg
     6301 cgggtcccgc ggcggtcgaa ctccgccagt gccgcctcga ctaggcgccg ggcgatgccg
     6361 cgatcccaaa gatccgggcg caccgagagc ggcccgaaaa agccgacgct gcccgagtcg
     6421 accacgaact tggtgccaac gacctcgccg cccagatcgc ccgcaacgct gcctgctgga
     6481 tcggccaggt accgggtccg caccgggttc gcgtcgccgc ggaaagtcag cgggtctggc
     6541 aggccgagga aggtgccgaa ggccaggcga cggaccgtgt cgaccgcggc caggtcgtcc
     6601 gtccgcaacg gacggacgcg caggtcggtt ccggctcccg tttcgcctgg cgtggtctcc
     6661 atctcgcggc ctccttggtg gttgtgtgat tgatgcgaat cagcgtggca ttttgccgac
     6721 cttcgaatcc tcctcggcat gcgaagacgt tagcgggcat tatccgtgac accgatcacc
     6781 gatgcccagc cttctgggcg ttgagcgcca gcagtcgacc gaggatgccg tcctcctcgt
     6841 cttccaccag ggcctcccca tccggccagc cataggcatc ccagaccgcg gggtcgcggg
     6901 cggtgctggc ctgccacttg ggtcggaagc agggatgggg tcacggcgat cggacggccc
     6961 gccactcgtt cggctcgagg accaacgtca ccgtcatccc gatcacgagc gcccagaacg
     7021 ccgatcccaa cccgccaagt gacaactccg acgaggcgac cacgaaggca aagagcgggc
     7081 cgagccgcag cggtccgttc accatctccg tcaaggcgct gctgagcacc gagaac
//
LOCUS       DASYWL010000225         6081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_123312, whole genome shotgun sequence.
ACCESSION   DASYWL010000225 DASYWL010000000
VERSION     DASYWL010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6081
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123312"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VGR22_07920"
     CDS             <1..452
                     /locus_tag="VGR22_07920"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HEV2128528.1"
                     /translation="KTTRRSFSLGVAGFALATSMTVAPRGMTAQESTPEVTDMGNAVT
                     ALGIGEEGDAAVNVVHTSPDTPAVDVYLDGEQALTGLAFGEYSGWLALPAGEHQIQVT
                     AEGAELDAAVIDTTVTVEEGWPTRSPPPVSSRRSCTDLPGRPQHARG"
     gene            469..768
                     /locus_tag="VGR22_07925"
     CDS             469..768
                     /locus_tag="VGR22_07925"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HEV2128529.1"
                     /translation="MIHASPDAPAVDIAVADGDVLIESLEFPNASDYLEVPAGTYDLE
                     VCPAGTTDVALDLPGVELEAGMVYDVFAIGQLADETLTVLIVPATTTAAQATPAS"
     gene            complement(1105..1461)
                     /locus_tag="VGR22_07930"
     CDS             complement(1105..1461)
                     /locus_tag="VGR22_07930"
                     /inference="COORDINATES: protein motif:HMM:NF015004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HEV2128530.1"
                     /translation="MRIWDVPPAILCRQHLLGEHRELHGLWNILTQGKRGYSHHPETV
                     RWQGKLSALYRRHEALVTEMKRRGYRHGTPLDPLLATGLPDQTDFVDPIPHQYELLRE
                     KPCDCLIDGHATTTGI"
     gene            complement(1461..2411)
                     /locus_tag="VGR22_07935"
     CDS             complement(1461..2411)
                     /locus_tag="VGR22_07935"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEV2128531.1"
                     /translation="MNERQNGRQPKSDLLPIGSVVRRLQETHPSVSQSSLRFLEREGL
                     IEPTRTPGGHRLYSEADIERIQQIKAWQDQRLSLDQIRERLEQRDRLPHPGDLADGFL
                     QLILSAQLADARALLLHADSLGMPLAALFGEVLQPVLYEVGLRWEDGRMLVAQEKETS
                     ELARDLIAELSQRHDATQPDGPSLVAGSVQGERHELGLRMISGLLRSRGCRVHYLGAD
                     VAHEFFIEAVELHQPDAVLISVKLEANVEQVGAAAERIRAALAADPAPPILAGGEAVA
                     SSADHLRQRGVYPIVDTTLDGVIDTVMGKLPSSAIGQDVI"
     gene            2807..2995
                     /locus_tag="VGR22_07940"
     CDS             2807..2995
                     /locus_tag="VGR22_07940"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HEV2128532.1"
                     /translation="MVHASPDAPLVDIYVDGQQVLTCLAFGWWSGWLALPAGEHQIQV
                     TATGAALETAVIDATLTS"
     gene            3473..4912
                     /locus_tag="VGR22_07945"
     CDS             3473..4912
                     /locus_tag="VGR22_07945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEV2128533.1"
                     /translation="MAPTTRIYRTPKPATPEQLYSWHDSPGAFHRLSPPWREVEVEEV
                     VGTIRPGDRKRLRIPLVGPIGVTWDLVHTDDVDSLGFVDTQVKGPFRSWRHEHHFWSD
                     GNGHAVLEDRVEYGLPMGPLGRVVVGSRVNAEMDRLFELRHTRTRIDLQRLVRNRKDT
                     PMRIAITGSTGLVGERLVAFLRGGGHDVLRLVRSEPKASDEISWSPSTGEIDAESLEG
                     LDAVVHLAGVSISSGLWTEKRKQAILQSRVEGTQLLARTLAGLQSPPKVLVTASGVNY
                     YGDTGNRETTESAPGGEGFLADVVRQWEAASEPAKVAGIRVVNARFGVVMAGEGGMLP
                     LIALPFRFGVGGPLGSGKQWMSWIALDDLVGVIHEAIINEELSGPINATSPEPVTNEE
                     FTKTLGTVLKRPTFLRVPAFAARTVGGQLAEELILISLRVVPARLQEIGFHFAFPTLE
                     AALRHELGRYVGHRASEEPAAEDREDIAA"
     gene            4962..6029
                     /gene="fni"
                     /locus_tag="VGR22_07950"
     CDS             4962..6029
                     /gene="fni"
                     /locus_tag="VGR22_07950"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEV2128534.1"
                     /translation="MTDGSSATGQRKSDHIRINLEQQVQARETTSGVERYRFLHNALP
                     ELDLREVNPATSVLGRELGAPLLISSMTGGIERGWEITRRLAIAAQEHGCAIGVGSQR
                     AAIEDGSRARFFAVRDVAPDVLLFANLGAVQLNHGFDVAECRRAVEMIDADALILHLN
                     PLQEALQPEGNRDFSGLLCKITKVCDALEVPVVVKEVGFGISADVARQLHDAGIAAID
                     TSGAGGTSWSAVEHHRAGDDHYRRLSETFVDWGIPTAESLRMVRESLPNAVLFASGGM
                     RTGLDAAKAVALGADLVGFAGPMLKAAADSEAAAVAMLRVLIEELRLAMFATGSRTLC
                     DLRHVPLLCVSGARLPLSDLR"
BASE COUNT         1004 a         1942 c         2041 g         1094 t
ORIGIN      
        1 tgaagactac ccgacgatcc ttctctctcg gcgttgccgg gttcgcgctt gccacgtcga
       61 tgaccgttgc gccgcggggc atgaccgctc aggagagcac gccggaagtg accgacatgg
      121 ggaacgccgt aacggcgctc ggcatcggcg aagagggcga cgccgccgtc aacgtggtac
      181 atacctctcc ggacaccccg gctgtcgatg tctacctcga cggtgagcag gccctgaccg
      241 gtcttgcgtt cggtgagtac tcgggctggt tggcattgcc ggctggcgag caccagattc
      301 aggtgaccgc cgagggtgcg gagctcgatg ccgccgtgat cgatacgacg gtcactgtcg
      361 aagagggctg gcctaccagg tcgccgccac cggtctcctc gaggagatcc tgcacagatc
      421 tacccggtcg acctcagcac gctcgaggat gacacggccc gggtccgggt gatccacgcc
      481 tcgcccgacg cgccggccgt cgatatcgcc gttgcggatg gtgacgtcct gatcgagagc
      541 ctggagttcc cgaacgcgag cgactacctc gaagttccgg caggcaccta cgaccttgaa
      601 gtgtgcccgg ccggcaccac tgacgtggcg ctggatcttc ctggcgtcga gctcgaggcg
      661 gggatggtct acgacgtctt cgctatcggc cagctcgccg acgagaccct gacggtgctg
      721 attgttccgg ccaccacgac cgctgcgcag gcgactcctg cgtcctgacg ttcattccgc
      781 cgggatgcgg gctacctcct tcccacccgc atcccgagtt cttaccccat ctggaccgga
      841 cgccgcgcga gtggcgtccg attgccctcc ttgcctccgg gcacggcgac tgaggcgcac
      901 tcgcgcagag ctgttcgctc tgctggtcgc tgtactggac aggccgacgg tctcgagcca
      961 ggtgactgcg tcgccagccg agcatcgccg gcccttgtcg tcttgaatgc ggctgggccg
     1021 gggtgagacc acgtgtcctt ggagcggatt gggtacgagc cacagatgcc acggtggcga
     1081 acgggcgccc tacgagaacc gaaatcagat gccggttgtg gtggcgtgac cgtcgatgag
     1141 acagtcgcag ggcttctcgc gcagcagctc gtattgatgt gggatggggt cgacgaagtc
     1201 tgtttggtcg ggcagcccgg tcgcaagcag tgggtcaagc ggcgtgccgt gccgatatcc
     1261 gcgccgcttc atctccgtca ctaacgcttc atgccgtcgg tacagcgccg acagcttccc
     1321 ctgccacctg accgtttcag gatggtggct gtagccgcgc ttcccctgcg tcagaatgtt
     1381 ccacaggccg tgcagctccc ggtgctcgcc gagcaggtgc tgccggcaga ggattgccgg
     1441 cgggacgtcc cagatgcgca tcagatcacg tcctgtccaa ttgccgagct tggcaacttg
     1501 cccatcacgg tatcgatcac accgtcgagg gtcgtgtcca cgatcgggta gacaccccgc
     1561 tggcggaggt ggtcggccga tgaagcgaca gcctcgccac cagcgagtat cgggggtgcg
     1621 gggtcggccg ccagcgcggc gcgaatgcgt tcggcggccg cgccgacctg ctcgacattg
     1681 gcctccagct tcacggagat cagcaccgcg tcgggctgat ggagctcgac tgcctcgatg
     1741 aagaactcgt gggcgacatc tgcgcccagg tagtgtaccc ggcacccgcg ggagcgcagc
     1801 aacccgctga tcatccgcag gccgagctcg tggcgctcgc cctgaacgct cccggcgacg
     1861 agcgagggtc cgtcgggctg agtcgcgtca tgtcgctggc tcagctcggc gatcaggtcg
     1921 cgcgccagct cggaggtttc cttttcctgg gcgactagca tgcgcccatc ttcccaacgg
     1981 aggccgacct cgtacagcac gggttgtagc acctcgccaa acaacgccgc gagcggcatg
     2041 ccgagtgaat cagcatgcag cagcagggcg cgggcgtctg cgagctgcgc ggagaggatg
     2101 agctgcagaa agccatcggc gagatcgccg gggtggggca ggcggtcgcg ttgctcgagg
     2161 cgctcgcgga tctggtcgag cgaaagacgt tggtcctgcc aggccttgat ctgctgaatg
     2221 cgctcgatgt ccgcctcgga gtagagccgg tgaccgcctg gcgtgcgagt cggctcgatg
     2281 agcccctcgc gttcgagaaa ccggaggctc gactgggaaa cggatgggtg tgtttcctgg
     2341 agccggcgca cgacactccc gatgggcagg agatcactct ttggttgtcg accgttctgg
     2401 cgctcgttca acttcaacct ccagtcgaga gtggtgtagc acgccctccg gcatgctcgc
     2461 catcgccgcc cttcacaagg gttggcctcg ggagttgcgc ctcggatttc cctcttgaaa
     2521 agtataaacc taaactgtat attaatgtat agacatggca cggatggccc ctccgctggc
     2581 cggcagtgac tcgaaccgac gagacagcag gtacaaggag ttcacaatga gcagtcgaac
     2641 gttcccacgg aaaatactct cgatcaccgg cttgctggtg ttgatgctga tgatggtgac
     2701 cgctggtcgt ggcgtcgccg cctaggaaag cactcccgct gcgagtcact tgggcagctg
     2761 tactgctgct ctcgggatcg gctcggaagg cgatacctgc gtcagcgtgg tgcatgcctc
     2821 cccggacgcg ccgctcgtcg acatctacgt cgacggtcag caggtcctga cgtgcctcgc
     2881 gtttggctgg tggtcgggct ggctcgccct tcccgcgggc gagcatcaga tccaggtgac
     2941 ggccacgggc gccgcgctgg aaaccgccgt gatcgacgcg acccttacgt cttgaagccg
     3001 gcgaggccta ccaggtcgct gcaacgggct tcctcaacga gatcgctccg cagatctacg
     3061 gggccgatcc cagccagctc gccaacgata cggcccgcct gcgtgcgatt cacaccgttc
     3121 ctgacgcgcc ggccatcgat gtcgccgtcg ctggcggtga tgtcctgatc tcgaacgtcg
     3181 agttcccgaa cgccagtgac tacatcgaag ttccggcgga cacctacgac cttgaggtcc
     3241 gcccggctgg caccatggac gtcgccttgc cgctgtctgg cgtcacgttc gaggccggca
     3301 tggtctacga tgtgttcgca atcggtcagg tctccgacgg cacgcttaac gtgctcgtgg
     3361 tctcatctat ggcagcgcac ggcgcgcagg ccggaagggt ccggattgcc cgcgttaatg
     3421 gatgcgcgga cgggcaaccg catggtccgg gcatcgtcgc tggagagaca ccatggcacc
     3481 caccacgcga atctaccgca ccccaaagcc cgcgacgccc gagcagctct acagctggca
     3541 tgacagcccg ggtgcattcc accggctctc gcccccatgg cgagaggtcg aggtagagga
     3601 ggtcgtcgga accattcgtc ccggggaccg caagcgcttg cgcattccct tggtgggacc
     3661 gatcggggta acctgggatc tcgtgcacac cgacgatgtc gactcactcg gctttgtcga
     3721 cacgcaggtc aaggggccgt tccggtcgtg gcggcacgag catcactttt ggagcgatgg
     3781 caatggccat gcggtattgg aggatcgagt tgagtacggg cttccgatgg gcccgcttgg
     3841 acgggtagtc gttggaagca gggtcaacgc tgaaatggat cgcctcttcg agctgcgaca
     3901 cacccgtacc cggatcgatc tccagcgact cgtccggaat cgaaaggaca cacctatgag
     3961 aatcgcgata actggctcca ccggactggt cggcgagcgg ctggtggcat tcctgcgagg
     4021 cggcgggcat gacgtcctgc ggctggttcg ctcggaacca aaggccagcg atgagatctc
     4081 ctggagcccg tcgacgggag agatcgatgc cgagtcgctc gagggactcg acgcggtcgt
     4141 ccacctcgcc ggggtctcca tttcgagcgg gctgtggacg gagaagcgaa agcaggcgat
     4201 cctgcagagc cgcgttgagg gcacgcagct gcttgcccgc acgctcgccg gattgcagtc
     4261 gccacccaag gtgctcgtca ccgcatcggg cgtgaactac tacggcgaca ccgggaatcg
     4321 cgagacgacc gagtcggcgc ccggtggtga gggcttcctc gctgacgttg tgcgccagtg
     4381 ggaggcagcg tccgagcccg cgaaggtggc cggcatccgg gtcgtcaacg cccggttcgg
     4441 cgtggtgatg gccggcgagg gcggtatgct gccgctgatc gcgctaccgt tcaggttcgg
     4501 ggtcggtgga ccgctcggca gcggcaagca gtggatgagc tggatcgccc tcgacgacct
     4561 ggtcggcgtc atccacgagg cgatcatcaa cgaggagctc tccgggccga ttaacgccac
     4621 gtcgcctgag ccggtcacga acgaggagtt tacgaagacc ctcggcaccg tcctcaagcg
     4681 gccgaccttc ctgcgtgtgc cggccttcgc tgcccgcacc gtcggcggcc agctggccga
     4741 ggagctcatt ctgatcagcc tgcgggtcgt tcctgcccgg ctccaggaga tcggattcca
     4801 ctttgccttc ccgacgctgg aagctgcctt gcgacacgag ctgggccggt atgtgggcca
     4861 cagggcgtcc gaggagccgg cggcggaaga tcgcgaggac atcgctgcct gatggtgccc
     4921 atcgattggg ctgcgtgtga gggtgacagg ggagtgagag cgtgacagac ggatcatcgg
     4981 cgacgggcca gcgcaagtcc gatcatattc gtatcaatct cgagcagcag gtacaggcgc
     5041 gtgagacaac aagcggcgtc gagcgctacc ggttcctcca caacgccctg ccggagctgg
     5101 atctgaggga ggtcaaccca gccacgagcg tgcttggccg cgagcttggc gcgccgctcc
     5161 tcatctcctc gatgaccggt gggatcgagc gcggctggga aatcacccgc cgtctggcga
     5221 tcgctgcaca ggagcacggt tgcgcaattg gcgttggctc gcaacgggca gcgatcgagg
     5281 atggaagccg cgcgcggttc ttcgctgttc gggatgtcgc ccctgatgtg ctgctcttcg
     5341 ccaacctcgg cgccgtgcag ctcaaccacg ggttcgacgt cgccgaatgc cggcgcgcgg
     5401 tcgagatgat cgacgccgat gcgctcatcc tgcacctcaa tccgttgcag gaggcgctgc
     5461 aaccggaggg gaaccgcgat ttctccggcc tgctctgcaa gatcaccaag gtgtgcgacg
     5521 cgctggaggt cccggtggtg gtcaaggagg tcggcttcgg catctccgcc gatgtcgccc
     5581 gacagctcca cgatgccggg attgccgcga tcgacaccag tggcgccggc ggcacatcgt
     5641 ggagcgccgt tgagcatcac cgcgccgggg acgatcatta ccggcggctg agcgagacct
     5701 tcgtcgactg gggcatcccg actgcggaga gccttcggat ggtgcgcgag tcgctcccaa
     5761 acgccgttct cttcgccagc ggtgggatgc gcacgggtct cgatgctgcc aaggcggtgg
     5821 cgctcggtgc agaccttgtc gggtttgccg gcccgatgct gaaggcggcc gcggacagcg
     5881 aggcggcggc cgtggcgatg ctccgtgtcc tgatcgagga gctccggctg gcgatgttcg
     5941 cgacgggctc gcgcacgctc tgcgacctcc gacacgtacc gttgctctgt gtcagcggtg
     6001 cgcgtctgcc cctgagtgac ctccgctagt cgcaggttgc ccccttaagg ccatcagccc
     6061 tgatcatgtg gcaacggtgg t
//
LOCUS       DASYWL010000226        21913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_573785, whole genome shotgun sequence.
ACCESSION   DASYWL010000226 DASYWL010000000
VERSION     DASYWL010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 21913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21913
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573785"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VGR22_07955"
     CDS             complement(<1..644)
                     /locus_tag="VGR22_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128535.1"
                     /translation="MSELRTKVPDAVDTTEDSDAIEPIRTIIALFDDTIDAEHALTDL
                     RRTNQPSAAISVILRERVSHRDSNITRETLLSRVVAASALEAVGGWLQGLASLILPED
                     RASYLVAGPIGVMLASIHETHDMDENDETEDTTYRVTSRQLTRALTLFGFEVDEATYV
                     EHRVVAGSPLIAVTAEHAPVLRDAHEIFSGNTAVHIGLTHTDRSIQSVAARLLMT"
     gene            complement(641..1843)
                     /locus_tag="VGR22_07960"
     CDS             complement(641..1843)
                     /locus_tag="VGR22_07960"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV2128536.1"
                     /translation="MATGTSFVRIILYTAAVLAVLGMVWFVVQARSILVLLILGIIFG
                     TAIEPLVFRLRRSGLSRGQSIMVIYLAIFAIIGTAVYLVVPILVRQVTAFDAAVPEIF
                     ENLRAQALELDNALIRRTAYQTLWRIQNGYEQIRSGPEIGQDQALGLATSVFGILLSL
                     FSLLIIAYYWMTEKATIKRLVLGLIPFPRRERVHTIWDEIEYKIGGWTRGQLLLMVMI
                     GVSSGVIYYFLDLRFWLALAIWAGLTEAIPFIGPFIGGGTATLIALADSPEKAGLVIL
                     ATFLLQQLEGAILVPRVMRNAVGMSPLTVVLAVLIGNALAGPIGSLLAIPIGAATQVV
                     VQNLLRLRDDIITTELRTMDITPLSETAVNSPFAEQTDEADQEHHRHYPLVGPDPRPR
                     LQGRREHG"
     gene            complement(1944..3380)
                     /locus_tag="VGR22_07965"
     CDS             complement(1944..3380)
                     /locus_tag="VGR22_07965"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEV2128537.1"
                     /translation="MSSKTTKSRLVIDFRNATNVVVGERHGVSTAELEEISQTVSEQH
                     DRLMREHGAGEQRWMDLPDDTGLADEIVAFAGDARNRYDDFILVGIGGSSLGAIATVQ
                     ALAHPYRNMLPKERRGGPRIFVLDNPDPEKIAATLDTVDLSRTLVNVVTKSGQTAETM
                     ANFLVVRDALIQALGEEQARQQIVATTDPESGLLRQLADQENYRTFPVPPGVDGRQTV
                     LSAVGMLPAAMAGVDVHGLLAGAAAMRERCQTGNLLENPAYLLAGLAWLADTEHDKSM
                     LVTMPYADALFGISDWFRQLWAESLGKKLSVDNEVVYAGQTPIKALGAIDQHSQIQLY
                     TEGPNDKLIQLIAVGTYRETVNIPTPPAGIPELSYLQGGELGELLDKERVATAWALAK
                     ADRPNLTITTPLIDAFAVGEFFYLMELQTVMAGALYNVNPFGQPGVEAGKNATYALIG
                     REGYEDLRAELEAPVSEAERFLNAPVGD"
     gene            complement(3749..4849)
                     /locus_tag="VGR22_07970"
     CDS             complement(3749..4849)
                     /locus_tag="VGR22_07970"
                     /inference="COORDINATES: protein motif:HMM:NF037640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEV2128538.1"
                     /translation="MPTTDALDSPDMLVIGLGTAGRAVLDAAYAVGVQAIGVDQSRLS
                     TDVSPNLAFDVTRAWGVFSDGTVVLAGPTVRWPCKPKAIVVATGSLDLPLPMPGWELD
                     GVTGAHHARERLPDGARVVVIRGPHAGLGNRQPDLSMFQVIADRDVAGDAPVTIIGDG
                     HVEAVVIGDDQLETSYVLLDNGLQPENILARMAGVPNLFSAAAGGDVIVPGAVFATGG
                     ALLSLVGDAAGIHSDRERVVRDAAETGQVLAESVLTGHIPVSTISERFAWPAGGAPVL
                     PSQATFDTLVCPSEGVTVGMVREAIARGAITVNDVKRRTRAAMATCQGRDCLWTTRAM
                     LAEANRNWETPMTARPPAVGITLQELAGIAGD"
     gene            5061..6455
                     /locus_tag="VGR22_07975"
     CDS             5061..6455
                     /locus_tag="VGR22_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEV2128539.1"
                     /translation="MSTNVDEKTRTSAPETVEQEALEHVWIHTRSWSELAEQQGLKVF
                     SRGERSTIWDVHGKEYLDGISGLWVVNAGHGRAEIGQAMAEQAGKLAYASAEAFTTEP
                     AVELAHTLAELTPGDLSRVYFSSGGSEAVETALKIAKQVQAMRGFPRRYKIIARRGSY
                     HGMTHGAMSLTAMREEKYFGPFMYGVSHVPHPNRYKSDFGLEGEEADIMAAKYVEQEI
                     INQGPETVAAVIGEPISSAAGVHVPSKKYWQMLREICDRHGVLLIADEVINGFGRTGT
                     MFATEQFEFVPDIMTMAKGLTSGYAPMAATVVRPAVFESFKEQADVAMAHLLTFGGHP
                     VCAVAALRNLQIFEDENLVQRGAEMGAYLKERLEELRAHPTVGDVRGVGLLQAIELVK
                     SKETKENFPKTHRFAKRVTGMLADRGLITRVREAMHFAPPLVVTKDEIDRMVTITDEV
                     LTVAEDEFAQDIDS"
     gene            complement(6565..6975)
                     /locus_tag="VGR22_07980"
     CDS             complement(6565..6975)
                     /locus_tag="VGR22_07980"
                     /inference="COORDINATES: protein motif:HMM:NF024843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxC-x17-CxxC domain-containing protein"
                     /protein_id="HEV2128540.1"
                     /translation="MLSAEHSTTGGHRSEQRTCGDCGDTYAYTETEQRFRSEQGMKLP
                     TRCPQCRADRRAARNADLVAGETASRSRARRQRERRPEPQGRGGHRHGGNGFSGERYP
                     AVCADCGQSTMVPFVPRGDRPVYCRSCYSARRGR"
     gene            complement(6977..7183)
                     /locus_tag="VGR22_07985"
     CDS             complement(6977..7183)
                     /locus_tag="VGR22_07985"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEV2128541.1"
                     /translation="MTEHRFRVPGMSCGHCELAVKSELLKVDGVYNVDVNLETKTVKV
                     DHDAVVSTVAMRRAIEEAGYEIEG"
     gene            7431..9287
                     /gene="pepF"
                     /locus_tag="VGR22_07990"
     CDS             7431..9287
                     /gene="pepF"
                     /locus_tag="VGR22_07990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV2128542.1"
                     /translation="MATAMNEIPTRDQIKVEDTWDLRTIFVSDEGWEQEAQEIPGLVE
                     VAASYEGRLGEGPAVVAEALKAINLLEERLSMLLVYASLRRDEDTTDTEANARYEQAI
                     ALAIGASQALAFVQPELLALPREEFAQLASAEELSTYRHMFDNLDRQRAHVRSQEVEE
                     VLAQMGEVTRTASEAFNALDNADLEYGEVADEQGNTIALTKGRYGLLQESQDRDVRRE
                     SHDRMIEAYQRHEHTISSLYGASVRKDVTSARIRGYDSARREALFDDNVPDSVYDTLI
                     EVVRQSGPILERYLDLRRQALGVDRLEVYDLRVPLAPESTKHYDFRDAVEIVLRGVQA
                     LGEEYVRDLRSGFESRWVDVHETKGKRSGAYSWGAYGAPPVMLMNWNGTLSDVFTLAH
                     EAGHAMHSFYSNRAQPFHLASYPIFLAEIASTVNEVLLTWDLLGSDEARDPVQRFALL
                     NRFADSFYGTVFRQAMFAEFEQRTHAMVEAGTPLTLDALNQLYGELHEAYLPGVNVDD
                     KVRVHWSRVPHFYRAFYVYQYATGMSSAINIATAVRDEGTPAQQRYLEMLEAGGSDYP
                     MNILKRAGVDLTQREPLETAASEFERVVAEMERLAQDGVLTRAAEQAARQTA"
     gene            complement(9284..9610)
                     /locus_tag="VGR22_07995"
     CDS             complement(9284..9610)
                     /locus_tag="VGR22_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128543.1"
                     /translation="MRRRFTLPPHLRGLGEPIERFAQANIALVIVQAFIIRLSIALSI
                     AETDEPDMAVLLGMLAAAAAAGALVVVLRRVIRRFPRRSTRIALALGSLAFQALAFQL
                     IYSYAS"
     gene            9707..10342
                     /gene="eda"
                     /locus_tag="VGR22_08000"
     CDS             9707..10342
                     /gene="eda"
                     /locus_tag="VGR22_08000"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEV2128544.1"
                     /translation="MGTVERIEQSGVVAIVRLDDYSKATEMAEALAAGGVEAVEFTYT
                     NPAAGEAVLATKAALGDRMLVGAGTVLDPETARAAILLGADFIVTPTVQIETIRLCNR
                     YSIPTVIGAFTPTEILTAWEAGASVVKVFPASVGGPRYLKDVRGPLPHVKLIPTGGVS
                     EENAADFIRAGAIAIAAGSNLLDATTVEQGDWAKLTDRARTLVAAVREAHS"
     gene            10444..10740
                     /locus_tag="VGR22_08005"
     CDS             10444..10740
                     /locus_tag="VGR22_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128545.1"
                     /translation="MTDDTIRLPLLKRLAEQAMTDGAFRAVAREDLARALGEYGYDLN
                     DRERAFVFRFRQALEEANVDLELAKEVDLDALLAADDDHATGLEGLLSSIERTG"
     gene            complement(10845..11837)
                     /locus_tag="VGR22_08010"
     CDS             complement(10845..11837)
                     /locus_tag="VGR22_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEV2128546.1"
                     /translation="MTLPHVAILTTGGTIASLRDPETGAVRTAATPEDLLELVPDISE
                     IAEMSAYPQAAVNSWNMSPTMMLDLITSAIAKLVNEKISGVVITHGTDTVEETAILAW
                     MLNPTEEPIVFTAAMRNLSETGPDGPRNLRDAVRVAACPEARGRGAMLVVNETIHDAR
                     YVTKTSTVNPATFESPHGGPIGEATAVGVEFFRPPVERKALEQPRIGGTVPIVKAYTG
                     MDAAVLDWYRDRGAEGVVIEGSGAGNVPGEVLPGIERLTAAGVPVVLTTRCISGPLAP
                     VYGTGGASGGGHDLMRAGVIPASRFTAQKARICLMALLGSGASTSQIDAWFRSV"
     gene            complement(11834..12478)
                     /gene="upp"
                     /locus_tag="VGR22_08015"
     CDS             complement(11834..12478)
                     /gene="upp"
                     /locus_tag="VGR22_08015"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014471148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEV2128547.1"
                     /translation="MKQVHVSPHPLVQHKVTLLSDERTNSKTFRELVRELTRLLLYEA
                     TADLPLREVEYSTPLERTIGHEVGARIGLVPIIRAGLGMVEAAVDALPSAEVWHLGMY
                     RDEATHQPVPYYNKLPEECPDDVVLVLDPMLATGGSASDAISVLKAWGATSIRFVGII
                     GAPEGTARVTADHPDVPIHLARRDRGLNENRFILPGLGDAGDRLFGTLARGNES"
     gene            complement(12544..13344)
                     /locus_tag="VGR22_08020"
     CDS             complement(12544..13344)
                     /locus_tag="VGR22_08020"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEV2128548.1"
                     /translation="MPKLAANITMLFTEYPLADRFERAAANGFGGVEMLFPYEMRANE
                     VQDLLREHHLDLVLFNLPAGDWAAGDRGIAADTNRVDEFRNGVDKAVTYAEALKPRRV
                     NCLVGKADDVSATRSVMLENIVYAADALATVNTRLVIEPVNTQDVEGFAIPDTAAARS
                     VLAEIEHPNVGLQFDVYHSLRMGEDPLAIIRELGASLDHIQIADVPGRHQPGTGELDF
                     EQVFATIDSSGYTGWVSLEYNPAGHTEEGFGHLRELGYLDTSASRQDR"
     gene            13523..14971
                     /locus_tag="VGR22_08025"
     CDS             13523..14971
                     /locus_tag="VGR22_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2128549.1"
                     /translation="MSRTFGQVQACTDVSFGVGRGEVVALLGENGAGKSTLLSIIAGF
                     VVPDSGVVRVDGRLLEHGNPQAAIRAGIGTAFQHFSLSPELTVAESFALARVDVGEAR
                     RHLPDHIDMGTRTGDLGVPEQQQVEFVKARLLSARVMLLDEPTSLLGGTDVERVLDGI
                     QAAADAGIACVFVTHRLREALAVADRIVVMRRGWVVERMVRSDAGWPADTERQVLAAM
                     FGDVNAVDEPDVPRTWQSGPGVGTISVQGRVGERTRDISLQIGQVLAVAGIAGNGQEE
                     LIELLVGTTADRVRLETTGASLDLTGHRMHEWVRHNVAVVPEDRQLEGGAPAMSLGEN
                     LVLRDLAGGRLSTLGAVSRRRLRSRARGLIETWSIRPADPSVRFGSLSGGNMQRALLA
                     RSLDPLPKLLVAVRPAHGLDHRSVEMVRRELRAAADAGTAVVTVEGDLDEALAHADSI
                     AVMYQGQLSAAVPVAHADRTALQSMTVSGWDI"
     gene            14959..15996
                     /locus_tag="VGR22_08030"
     CDS             14959..15996
                     /locus_tag="VGR22_08030"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128550.1"
                     /translation="MGHLTRDIAVPLLAVLGAALIGVSLLAATGHDPLAVGEAFGRRV
                     LLRGAGIEESAVAMGPLLIAALAAWIAARVGLWNIGIDGQVIAGAIAAATLAPFLDTW
                     PRESMWVAVTVVAIVAGALWALLPALLRAHAGINEIVTTIMMTYVAFSLGSWLVEGPL
                     RDEALVTPVTVAIDVGRRLPRVFDTRIHLGVAAAVVIVLLTAAAARWTSPGILSRLVG
                     ESAPAAHRLGLPVERYVVVAFLVSGGVAALAGVAEVLATRGAVQGDWRPVLTLPAFAA
                     LFLARQNVLALLPAAFLLGMLAYASTVLPRATDLAPDFFPMLEGVLLILLAVERWQRP
                     ERAQAPAERSI"
     gene            15999..16913
                     /locus_tag="VGR22_08035"
     CDS             15999..16913
                     /locus_tag="VGR22_08035"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2128551.1"
                     /translation="MLETLLQASVLAAVPLMLAAVGEAIGQRSGLLNLGVEGVMLAGA
                     LVAFIAVETSGSFLAGLAAGALVGAVVGLLFGVASARMAASQIVLGLGIALAGQGATG
                     FFFRERYGLTQPLLEPGMSRPISDLSEVAVVGPALFGHKWFVYAAWAGVFLIAATLRF
                     TRAGLLLTATGDAPFATEAAGVDVTRVRVAAATAGQALAGLGGAALSIVEVGFFAPGM
                     TVGIGFIAIALAMVGRQDPLRIALLALAFGVLRGLGPAIQLTEWRVRVEFLDMIPYIG
                     VLVLVVVLGRRVRLPAALGVAYHREARP"
     gene            16961..18022
                     /locus_tag="VGR22_08040"
     CDS             16961..18022
                     /locus_tag="VGR22_08040"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEV2128552.1"
                     /translation="MMRRRASLILLILVGLVGPWPLGLAPAAQTEVDRAAIVTPGSRS
                     NEGWDQQGVDALVAIGEERGIEVDVVENAGYDDITPILRDLAADGADLIICHASGYQT
                     VCPEFAEREGVPVVVTENPEAVTEGLVSDVETEAQEVAYLAGVMAALETSTGTVAVVA
                     SGEPPTWNYMTAGFAAGAKDTDPSVEVLYSVIGEAAYDDAPNARRTTETVIAAGADVI
                     FGMGDGASFGMIQAIREHNAAVSQDEEVRFIDVIGDKSDSDVADFLLTSVLFDYTGAY
                     NTFIDNLESGDFGSIYTMDLENEGVWLLEPPIEVSEETIEQVEAAEQAILEGEIDVPA
                     IAEADELADFLEDLPEGDA"
     gene            18019..19203
                     /locus_tag="VGR22_08045"
     CDS             18019..19203
                     /locus_tag="VGR22_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEV2128553.1"
                     /translation="MTHEPRTDWPHLTLASGPVDVPLHTLRAMQRPIVYHYDPVFIEE
                     FRRVEALLKQVFRTEHDVIIMQGEAVLALEAAAASVIRQGTKILNLVSGVFGAAYAGW
                     IERYGGEMIEIRVPYDQAIDPEEVRRVLDEHPEIEVLAAVHCETPSGTMNPIGEIGRI
                     AQEYGVLTIVDTVSGLGAEVYSPEESGIDIAVAGAQKCLGGVPGLSLVAISPRAWEVM
                     ESVDSPLRGSYLSMLDWKDAWIDGERFPYTPSVADVHALESTLELAVEEGVEAMAVRH
                     QTIAAACRAAARGLGLELWPASDDIAAPGCTAVRVPKGTTAEAIIETMRSRYGVMISG
                     GYGDLSGKLFRLGHMGQSAHPTTLFAQIGVLERSLLDLGVSVTPGAGVGAAVGALAGW
                     GN"
     gene            complement(19263..19703)
                     /locus_tag="VGR22_08050"
     CDS             complement(19263..19703)
                     /locus_tag="VGR22_08050"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEV2128554.1"
                     /translation="MLSGIGVLIELYLVWVHYGLDALVCGSGDCEIVQTSSYATVMDI
                     PIAWFGLLMYLSLLALAGLRLQKPELMLQGSTAALAVTAAGTLYSGWLTWVELYVIEA
                     ICQWCVASAIITTLFFVIEIVVFRRLWSGTEDDDRERLDELAES"
     gene            complement(19751..19990)
                     /locus_tag="VGR22_08055"
     CDS             complement(19751..19990)
                     /locus_tag="VGR22_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128555.1"
                     /translation="MLEQAAVDLGLDMEAFNECLASGQKEEEALAFGEDALQRSVSGT
                     LTFLINDQLVTYTRGGFDTPEEQINQALAGERIDA"
     gene            complement(20066..20443)
                     /locus_tag="VGR22_08060"
     CDS             complement(20066..20443)
                     /locus_tag="VGR22_08060"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEV2128556.1"
                     /translation="MLLLEAGTLAIALVAVVALVLANQDDETEAPEAALALEEVENDG
                     RVLGDPDAPVDFVVYSDFQCPFCRQFDDQDLPQVVDTFVDTGDVRVEWRPLPIVSGFQ
                     NIPPEADENESVQSAEAALCAAD"
     gene            20740..21708
                     /locus_tag="VGR22_08065"
     CDS             20740..21708
                     /locus_tag="VGR22_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV2128557.1"
                     /translation="MTDQDNARRSVRSAKLERVLVPVDGSPASERALEYAAHLRPERI
                     VLLRVEVDEPVGPHVDPADPYERWRRDHLSTVMNELESLARSKAATADVVEPKIRFGN
                     PADRIMAEAHDHDLIVMGATGKGAAGRLLFGSVADRVMRNAITPTLVVRTNGDEAEIR
                     PPARVVVPLDGSELAERALPMATRVSAALSIPIRLVRCVGMDEVLHTVREARRSGDAE
                     LFESSDDPYKLGQERANREAEAYLEAVPERLGVAGQNVTCERLSGSAAFELLWSLQED
                     ELVVMTSRGRGGLQRWVVGSVAEKLVREAKAPVLLVPVGRAGMESE"
BASE COUNT         3745 a         6686 c         7547 g         3935 t
ORIGIN      
        1 gtcatgagca gccgggcggc gactgactgg atcgaccggt ccgtgtgagt caggccgata
       61 tggaccgcgg tgttgccgga gaagatctcg tgcgcgtccc gcaggaccgg cgcgtgctcg
      121 gcggtcaccg caatgagcgg cgaaccggcc acaacccggt gctccacgta ggtcgcctcg
      181 tccacctcga aaccgaagag ggtcagggcg cgtgtcagct gccggctagt cacccggtag
      241 gtcgtatcct ccgtctcgtc gttctcgtcc atgtcgtggg tctcgtggat gctggcgagc
      301 atcacgccga tgggcccggc aacgagatag ctggcacggt cctcgggcag aatcagcgag
      361 gcgaggccct ggagccagcc gccaaccgcc tcaagcgcgg aagccgcaac cacgcgcgat
      421 agcagtgttt cgcgcgtgat attggagtcc cgatgcgaca ccctctcgcg gaggatcacg
      481 gagattgccg cggacggctg gttggttcgt cgcaggtcgg tcagcgcatg ctcggcgtcg
      541 atggtgtcgt cgaacagggc gatgatggtc cggatcggct caatggcgtc ggagtcttcg
      601 gttgtatcca ccgcgtccgg caccttggtg cgaagctcac tcatccgtgc tcgcgcctcc
      661 cttgcagacg aggacggggg tcgggcccga ccagcgggta atggcggtgg tgctcctgat
      721 cggcttcatc cgtctgctcc gcgaacggcg agttgaccgc ggtttcggag agaggggtga
      781 tgtccatcgt tcgcagctcg gtcgtgatga tgtcgtcacg caaccgcagc aggttctgga
      841 caacgacctg cgtggcggcc ccgatcggaa tcgccaggag cgagccgatc ggcccggcca
      901 gcgcgttgcc gatcagcacg gccagcacga ctgtcagggg actcatgccg accgcgtttc
      961 tcatcacgcg gggaacgaga atcgcacctt cgagctgctg gagcaggaag gtggcgagga
     1021 tcacgaggcc ggccttctcg ggcgagtcgg cgagcgcgat gagcgtggcc gtgccgccgc
     1081 cgatgaaggg gccgatgaac gggattgcct cggtcaatcc ggcccagatc gcgagggcca
     1141 gccagaacct caggtcgagg aaatagtaga taacgcccga gctgacgcca atcatcacca
     1201 tcagcaggag ctgaccgcgg gtccagccgc cgattttgta ctcgatctca tcccaaatgg
     1261 tgtgaacgcg ctcccgtctc gggaagggaa tgaggccgag gacgagccgc ttgatggtcg
     1321 ccttctctgt catccagtag taggcgatga tgaggagcga gaagagcgag agcaggatgc
     1381 caaacaccga cgtcgcaagc cccagcgctt ggtcctgacc gatttcgggg ccgctgcgga
     1441 tttgctcata tccattttga atgcgccaga gggtctgata cgcagtgcgc cgaatcaggg
     1501 cgttgtctag ctcgagtgcc tgtgcccgaa gattttcgaa gatctccgga acggcggcat
     1561 cgaaggccgt aacctggcgc accaggatcg gcaccacgag atagaccgcg gtgccgatga
     1621 tcgcgaagat ggcgaggtag atcaccataa tgctctgtcc gcgcgacagg ccggatcgtc
     1681 tcagccggaa cacgagtggc tcgatggcgg tgccgaagat gatgccgagg atgagcagca
     1741 ccagaatcga gcgcgcctgg acaacaaacc acaccatacc gagcaccgcg agcactgcag
     1801 cggtgtagag tatgatccgg acaaaggaag tcccggttgc cacggctgct ccttcggggg
     1861 cctgtggata cgacaaagcc gaacgcgtcg cactcgcgcg ttcggctttg attctagccg
     1921 gttcgccgga actgtgaccg ctatcagtca ccaaccggcg cgttgaggaa ccgctcggcc
     1981 tctgaaaccg gcgcttccag ctccgcacgg aggtcctcgt acccttcgcg gccaatgagg
     2041 gcataggtgg cattcttgcc ggcctccacg cctggctgac cgaacgggtt gacgttgtag
     2101 agcgcgccgg ccatgaccgt ctgcaactcc atcaggtaga agaactcacc gacggcgaag
     2161 gcgtcgatga gcggtgtggt gatggtcagg ttcggcctgt cggccttcgc caaggcccac
     2221 gcggtggcca cccgctcctt gtcgagcagc tcgcccagct cgccaccctg aaggtaggaa
     2281 agttccggga ttccggctgg cggcgtcggg atgttcactg tctcgcggta cgtcccgacg
     2341 gcaatcagct ggatcagctt gtcgtttgga ccctcggtgt agagctggat ttgcgagtgc
     2401 tggtcgatcg cgccgagcgc cttgatcggc gtctgccccg cgtagaccac ctcgttgtcc
     2461 accgagagct tcttgccgag gctctcggcc cagagttggc ggaaccagtc cgagatgccg
     2521 aagagggcgt cagcataggg catcgttacc agcatcgact tgtcgtgctc ggtgtctgcc
     2581 agccacgcga gcccggccag cagataggcg ggattctcga gcagattgcc agtctggcag
     2641 cgctcgcgca tggctgccgc gccggcgagc aatccgtgaa cgtccacgcc ggccattgcc
     2701 gccggcaaca tgccgacagc actcaagacc gtctggcgtc cgtcgacgcc gggaggcacc
     2761 gggaaggtcc ggtagttctc ctggtcggcc agctggcgca gcagtccgct ttcggggtcg
     2821 gttgtcgcaa cgatctgctg gcgtgcctgc tcctcgccga gcgcttggat gagggcgtcg
     2881 cgcacgacga gaaagttggc catcgtctcg gcggtctgcc ccgacttggt gacgacgttg
     2941 accagcgtgc ggctcaggtc aacggtgtcg agcgttgcgg cgatcttttc agggtcgggg
     3001 ttgtcgagga cgaaaatgcg gggtccgcct cgccgctctt tggggagcat gttgcggtag
     3061 gggtgggcga gcgcctggac ggtcgcgatt gcgccgagcg atgagccgcc gatgccgacg
     3121 aggatgaagt cgtcgtaccg gtttcgggcg tcgcctgcga aggcgacgat ttcgtcggca
     3181 agcccggtgt cgtccgggag atccatccac ctctgctcgc cggcgccatg ctcgcgcatg
     3241 agccgatcgt gctgctcgct gactgtctgg ctgatctcct ccagctcggc agtgctgacc
     3301 ccgtgcctct ctccaaccac gacatttgtg gcattgcgga agtcgatgac caggcgggac
     3361 ttggtggtct tggaagacat gccgtactcc tcctcggtgg tgaaacgatt cgcggtggtg
     3421 tcattagacc gagcacgggc gctccggcct gtcggttctc tttgccctgg atagtagcaa
     3481 ctgcgatgcc gtgagcgtgg acagccaacc tgcgagagtc aggcaagcga attgcaccct
     3541 gcgcgaaacg acggattgct gcgaacagcg ccggcagctc ccgtgccgct gccgattacg
     3601 atgttttgcg cttcaggggt gccggccctc ccggccaaca tgctgcatat gtggggatgc
     3661 tccggctcgt cttggcagcg cgatacctcg ccggttcagg atgcatgtaa gcttcgaagg
     3721 atcgattgtg gctcgccctt cttgtggctc agtccccggc gataccggcc agctcctgaa
     3781 gggtgatgcc gactgcgggt ggacgcgccg tcatcggcgt ttcccagttg cggttcgcct
     3841 cggcaagcat ggcccgggtc gtccagaggc agtcgcgtcc ctgacaggtc gccattgccg
     3901 cacgggtgcg gcgctttacg tcgttgaccg ttatggcacc ccgggcgatc gcttcgcgaa
     3961 ccatgccaac ggtgacgcct tccgacgggc agaccagcgt gtcgaacgtg gcctggctcg
     4021 gcaggaccgg tgccccgccg gccggccagg caaaacgctc ggaaatcgtg gagacaggga
     4081 tgtggccggt cagaacgctt tcggcaagga cttgtccggt ctccgctgca tcgcggacca
     4141 cccgctcacg atcggagtga atgcccgcgg catctccaac cagcgagagc agagcgccgc
     4201 cggtagcgaa caccgctcct ggcacgatca catcgccacc tgccgcggcc gagaacaagt
     4261 tcgggacgcc tgccatccgt gcgagaatgt tctcgggctg caggccgttg tccaacagga
     4321 cgtagctcgt ctccaattgg tcatcgccaa tgacaacggc ctcgacatgc ccgtcgccga
     4381 taatggtcac cggggcgtca cccgcaacat cgcgatcggc gatcacctgg aacatcgaga
     4441 gatccggttg acggttgcca aggccggcgt ggggtccacg gatgaccacg acacgcgcgc
     4501 catccggtag tcgttcgcgg gcatggtgag cgccggtgac gccgtccaac tcccatcccg
     4561 gcatgggcag cggcaggtcg aggctgcccg ttgccaccac gatggctttc ggcttgcacg
     4621 gccagcggac cgttggtccg gccagtacca ccgtaccgtc actgaaaacg ccccacgcgc
     4681 gtgtcacatc aaaggcgagg ttagggctca cgtcagtcga gagacgagac tggtcaacgc
     4741 cgatcgcttg tacaccgacc gcgtaggcag catcgaggac ggctcggccc gcggtgccaa
     4801 ggccgatgac cagcatatcc ggagaatcga gtgcatcggt ggttggcacg taaagggctc
     4861 cttgaaccgt cgcattgcgc tccacgcggc tgtctgcgtg cttccacgct atatgccaca
     4921 gagcatggtc gtcaaggttg cgggccctgc ggcagatgcc cccgatggcg ctgctatact
     4981 ctcgccacat acccggtagc cggaccgttg acccacatcg cgtgccggca cccactatct
     5041 gcgtcaggag gaggcttcgc atgagcacaa acgtcgacga gaaaacgaga accagcgcac
     5101 ccgagacggt cgagcaggaa gcgctcgagc acgtctggat tcatacccgc tcgtggtcag
     5161 aactcgccga gcagcaggga ttgaaggttt tctcgcgcgg cgagaggtcg acgatctggg
     5221 atgttcacgg caaagagtac ctcgacggca tctccgggtt gtgggtggtc aacgcaggac
     5281 acggccgggc cgagatcggt caggcgatgg ccgagcaggc cggcaagctc gcctatgcct
     5341 ccgccgaggc cttcaccacc gaacccgcgg ttgagctggc ccacacgctc gccgaactga
     5401 cgccgggcga tctttcccgc gtctacttca gctccggcgg ctctgaggca gtcgagacgg
     5461 cgctgaagat cgccaagcag gtgcaggcga tgcgcggctt cccccgtcgc tacaagatca
     5521 ttgcccgccg cggttcctac catggcatga cgcacggcgc gatgagcctc acggctatgc
     5581 gcgaggaaaa gtatttcggc ccgttcatgt atggcgtctc gcacgtcccg cacccgaacc
     5641 gctacaagag cgacttcggg ctcgagggcg aggaagccga catcatggcg gcgaagtacg
     5701 tcgagcagga gatcatcaac caggggccgg agacggtggc ggcggtgatc ggcgagccga
     5761 tctcgtccgc ggcgggcgtg cacgtgccgt cgaagaagta ctggcagatg cttcgcgaga
     5821 tctgcgatcg ccatggcgtg ttgctgattg ccgatgaggt gatcaacggc ttcggccgca
     5881 cggggacgat gttcgcgacc gagcagttcg agtttgtgcc ggacatcatg acgatggcca
     5941 agggtctcac gtccggctat gcgccgatgg cggcgacggt cgtccgcccg gcagttttcg
     6001 agtcgttcaa ggagcaggcc gatgtggcca tggcccacct gctgaccttc ggcgggcacc
     6061 cggtctgtgc cgtggcggcg ttgcggaacc ttcagatctt cgaggacgag aacctggtgc
     6121 agcgcggcgc cgagatgggg gcctatctca aggagcgact ggaggagttg cgcgcgcacc
     6181 cgaccgtcgg cgatgtgcgc ggcgtcggcc tgctgcaggc aatcgagctc gtcaagagca
     6241 aggagaccaa ggagaacttc ccgaagacgc accggttcgc gaagcgggtc accgggatgc
     6301 tggccgatcg tggcctgatc acgcgggttc gcgaggcgat gcacttcgca ccaccgttgg
     6361 tggtgacgaa ggacgagatc gaccggatgg tgacgatcac cgacgaggtg ctgaccgtcg
     6421 ccgaggacga gttcgcccag gacatcgaca gctaggcaac tgccgggcac gaatggcaca
     6481 cggggtgggg attttggttg tccccgcccc gtgttcgctt cgcggctggt tgttgtcccg
     6541 tgcggctagt acaacggtgt atcgctatcg cccacgtcgc gcgctgtagc aggatcggca
     6601 atagaccgga cggtcgccac gcgggacgaa gggcaccatg gtgctctggc cgcagtcagc
     6661 gcaaacagcc gggtaccgct cgccggagaa tccattgccg ccgtgccggt gaccgccgcg
     6721 gccctgcggt tcgggacgac gttcgcgctg gcgcctggcg cgagatcgcg aagcggtctc
     6781 gccggcgacc aggtcagcgt tgcgggcggc gcggcgatcc gcgcggcact gtgggcaccg
     6841 tgtgggcagc ttcatgccct gctccgaccg gaatcgctgc tcggtctccg tgtacgcgta
     6901 agtgtcgccg caatcgccac aggtgcgctg ctcggatctg tggccgccag tggtggaatg
     6961 ctcggcagac aacacgctac ccctcgatct cgtacccggc ttcctcgatc gccctgcgca
     7021 tggcaacggt gctgacgaca gcgtcgtggt ccaccttgac ggtcttggtc tccaggttca
     7081 cgtcgacgtt atagacgcca tctaccttga gcagctcgct ctttacagcg agttcgcaat
     7141 gtccacagct catgccggga acccggaagc gatgttcagt catgctcatt tcgaaactcc
     7201 tcaagaatgc cttccgacca tacccgtgtg ggtgtacagg ctaccactca agcgattgta
     7261 tgccatcgct gcccgacctc ggtggcagcg tatgcattca gttcgccagt gggaaatgcc
     7321 cgttgcggcc ggaatggagg atacttgggg taacttgcga cctggctgct gacaccacgg
     7381 gcagcgccgg gcatgattga tgcagcgcgt tcccgtgacg aggagcatgc atggcaacgg
     7441 cgatgaacga gattccgaca cgcgaccaga tcaaggtcga ggatacctgg gatctcagga
     7501 cgatattcgt gagcgacgag ggatgggagc aggaggcgca ggagatcccg ggcctcgttg
     7561 aggtggctgc gtcgtacgag ggccgcttgg gcgaggggcc ggcggtcgtt gctgaggcgc
     7621 tgaaggcgat caacctgctg gaggagcgtc tctcgatgct cttggtgtac gccagtttgc
     7681 gccgagacga agacaccacg gacaccgagg ccaacgcccg gtacgagcag gcaatcgcgc
     7741 ttgcgatcgg tgccagccag gcgctcgcct tcgttcaacc cgagctgctc gccctgccga
     7801 gggaggagtt cgcacagctc gcctcggcag aagagctcag cacctaccgg cacatgttcg
     7861 ataatctcga ccggcagcgg gcgcatgttc gctcacagga ggtcgaggag gtgcttgccc
     7921 aaatgggcga ggtgacgcgg acggcatccg aggcgttcaa tgcgctggac aatgccgatc
     7981 tcgagtacgg cgaggttgcg gacgagcagg ggaacacgat tgcgctcacc aaggggcgct
     8041 acggactgct ccaggagagt caagaccgcg acgtgcgccg ggagtcgcac gatcggatga
     8101 tcgaagcgta tcagcggcac gagcacacca tctccagcct ctatggcgcg tctgtccgca
     8161 aggatgtcac gtccgcccgt atccgtggct acgactcggc acgccgtgag gcgctgttcg
     8221 acgacaacgt gccggacagt gtttacgaca cgttgatcga ggtggtgcgc cagtctggac
     8281 cgatcctgga gcgctatctc gatctccgca ggcaggcgct cggcgtcgac cggctggagg
     8341 tatacgacct gcgggtgccg cttgcgcccg aatcgacgaa gcactacgac tttcgcgatg
     8401 ccgtcgaaat cgtgttgcgc ggcgtgcagg cgctcggcga agagtacgtc agggatttgc
     8461 gaagcggttt cgaatcacgg tgggtcgacg tccacgagac gaagggcaag cgatccggcg
     8521 catactcgtg gggcgcctac ggagcaccac cggtcatgct gatgaactgg aacggcacgc
     8581 tctcggatgt tttcaccctg gcccacgagg ccgggcacgc catgcacagc ttctactcaa
     8641 atcgcgcgca gcccttccac ctcgccagct atccgatctt tctggcggaa attgcatcga
     8701 ccgtcaacga ggtgttgctg acctgggatc tgcttggcag cgatgaggcg cgggatccgg
     8761 tgcagcggtt cgcgctgctc aaccgctttg cggacagttt ctacggaact gtcttccggc
     8821 aggcgatgtt cgcggagttc gagcaacgca cccacgcgat ggtggaggcg gggacgccgc
     8881 tcaccctcga tgccctcaac caactctatg gggagctaca cgaggcctac ctgcccggcg
     8941 tgaacgtcga cgacaaggtg cgggtgcact ggagccgcgt gccgcacttc tatcgcgcct
     9001 tctatgtcta ccagtacgcg accggcatgt cgtcggcaat caacatcgcc acggccgttc
     9061 gtgacgaggg taccccggcg cagcagcgat accttgagat gctcgaggcg ggcggatccg
     9121 actacccaat gaacatactc aagcgggccg gggttgacct cacccagcga gagccgctgg
     9181 agacggctgc ctccgagttc gagcgagtcg tcgccgagat ggagcggctg gcgcaagatg
     9241 gggtcctgac gcgcgcagcc gagcaggccg cgaggcagac cgcttaggag gcatagctgt
     9301 agatcaactg aaacgcgagt gcctgaaaag cgagtgatcc gagcgccagc gcgattcgcg
     9361 tgctgcgtct ggggaagcga cggatcaccc gccgcagcac cacaaccagc gctccggcag
     9421 cggccgcggc agcaagcatc ccgagcagca ccgccatgtc cggctcgtct gtctccgcaa
     9481 tcgagagcgc gatcgacaac cggatgatga aggcctgtac gatcacgagc gcgatattgg
     9541 cctgggcgaa ccgctcaatc ggttccccga gtccgcgcag atgaggcggt agtgtgaatc
     9601 gtcgtcgcac gtcatctctc gcgttgtttc gtacccgtct tgctgcatgg taccgggaag
     9661 cggcgagaat gggcagcctg cggtatcccg aaaggacagg aatgcgatgg gcacggtgga
     9721 gcgaatcgag cagtctggcg tcgtggcgat cgtccgcctc gatgactaca gcaaggcgac
     9781 cgagatggcg gaggcgctcg ctgccggcgg cgtcgaggcg gtcgagttca cctacaccaa
     9841 cccggcggcc ggcgaggcgg tgctcgctac aaaggctgcg ctcggcgatc gcatgctggt
     9901 cggagccgga accgttctcg atccggagac ggcgcgtgcc gcgatcctgc tgggggcgga
     9961 cttcatcgtg actccgaccg tccagattga gacaattcgg ctctgcaacc gatacagcat
    10021 cccgacggtc atcggcgcgt tcaccccgac cgagatcctg accgcctggg aggcgggcgc
    10081 ctcggttgtg aaggtctttc cggcatctgt tggaggtccg cgctacctga aggacgtgcg
    10141 ggggccgttg ccacacgtga agttgatccc gaccggtggt gtgtcggagg agaatgccgc
    10201 cgacttcatt cgggcgggcg cgatcgcaat tgctgccggc agcaatctcc tcgatgcgac
    10261 gacggtggag cagggcgact gggcgaaact gactgaccgg gcgcggacgc tggtcgcagc
    10321 ggtgcgcgaa gcacattcct gagcggcggg gcaagccgcg ttggatagac tgaaacacag
    10381 aacgaccagt cggtaatccg ggcgccgatg cgacgggcgc agttgagatg aggcagtcgt
    10441 accatgaccg atgataccat ccgactcccg cttctgaagc gcctcgccga gcaggcgatg
    10501 actgacggcg cgtttcgagc cgtcgctcgg gaggacctcg cgcgggcgct cggtgagtat
    10561 ggctacgatc tcaacgaccg cgagcgagct ttcgtgttcc ggttccgcca agccttggag
    10621 gaggcaaacg tggatctcga actcgccaag gaggttgatc tcgacgcgct cctcgccgcc
    10681 gacgatgatc acgcgacggg gctcgagggc ctgttgagca gcatcgagcg taccgggtag
    10741 ctgacgggcg gtaaccaagg cgtagcccga gtgagggggt gcgagatgtc gcacagcggg
    10801 acagcgccga cctgtgcgct tttgaccttg ccaaggactc gccgtcagac ggaccggaac
    10861 caagcgtcga tctggctggt tgaggcacct gagccgagca gggccatgag gcatatgcgg
    10921 gccttctgtg cagtgaaccg tgatgccggg atgacgccgg ctcgcatgag gtcgtgcccg
    10981 ccgccggatg ctccgccggt accgtaaacc ggagccagcg gaccgctgat gcagcgcgtg
    11041 gtgagcacca ccgggacgcc cgccgcagtc agtcgctcga tcccgggcaa cacctcaccc
    11101 ggcacattgc ccgcccccga tccctcgatg acgacgccct cagcgccacg gtcgcggtac
    11161 caatccagca ctgctgcgtc cattccggtg tacgccttga cgatcggcac ggtgccgccg
    11221 atgcggggct gctcgagcgc ctttcgctcg accggcggcc tgaagaactc gacgccgact
    11281 gcggtggcct cgccgatcgg tccaccgtgc ggtgactcga aagttgctgg gttgacggtg
    11341 ctggttttgg tcacatatcg ggcgtcgtgg atcgtctcgt tgacgaccag catcgcgccg
    11401 cgcccccgtg cctcagggca ggcggcgaca cggaccgcgt cgcgcagatt gcggggaccg
    11461 tcaggacctg tctcggagag gttgcgcatt gccgcggtga agacgatggg ctcctccgtc
    11521 gggttcagca tccacgctag gatggccgtc tcctcgacag tgtccgtgcc gtgggtgatg
    11581 actacgccgc tgattttctc attcaccagc ttggcgatcg cgctggtaat caagtcgagc
    11641 atcatcgtcg ggctcatgtt ccagctgttg accgcagctt ggggataggc ggacatctcg
    11701 gctatctcgc tgatgtccgg gaccagctcg agcaagtcct caggcgtcgc ggcggtgcga
    11761 actgcaccag tctccgggtc acgcaggctg gcgatcgtgc caccggtcgt caggatggcg
    11821 acgtgaggca gggtcatgac tcgttccctc gcgctagggt accgaacagg cggtcacccg
    11881 catcaccgag accaggaagg atgaagcggt tctcgttcag cccgcggtcg cgacgggcga
    11941 ggtggatcgg gacgtccgga tgatcggccg tcacacgggc agtgccttcc ggcgcgccga
    12001 tgatgccgac gaagcggatc gaagtcgcgc cccacgcctt gagcacggag atggcgtcgg
    12061 atgcggaccc ccccgtcgcc agcatcggat cgagcaccag cacgacatcg tcggggcact
    12121 cttccggcag cttattgtag tagggcacgg gctggtgggt ggcttcatcg cggtacatgc
    12181 cgaggtgcca cacttcagcg ctcggcaggg catcaaccgc cgcttccacc atgccaagtc
    12241 cagcgcggat gattggcacg aggccgatgc gtgcgccgac ctcatggccg atggtgcgct
    12301 ccaggggcgt cgagtattcg acctcccgca gcgggaggtc ggccgtagcc tcgtagagaa
    12361 gtaaccgggt tagctcgcgg acgagttccc gaaaggtctt ggaattggtc cgctcgtccg
    12421 acagcagcgt gaccttgtgc tggacgagcg gatgaggact gacgtgaacc tgcttcatgc
    12481 atgtcccttc cggccaggtg gcgcgggcga ttcccttgac cgcgactcgg cggtctgcga
    12541 gggctaccga tcctggcggc tcgcggaagt gtcgagatat cccaactcgc gcaggtggcc
    12601 gaagccttcc tccgtgtgtc cagccgggtt gtactcgagg ctcacccatc cggtgtagcc
    12661 ggacgaatcg atggttgcaa agacctgctc aaagtcgagc tccccggtgc cgggctggtg
    12721 gcgccctggc acgtcggcga tctggatgtg atcgaggctg gcgccgagct cgcggatgat
    12781 cgcaagcggg tcctctccca tgcgtaggga gtgatagacg tcaaactgga ggccgacgtt
    12841 cgggtgctcg atctcggcga gcactgaccg tgctgcagca gtgtcgggaa tggcgaagcc
    12901 ctccacgtct tgcgtgttga ctggctcgat caccaagcgc gtgttcacgg tggccagcgc
    12961 gtccgcggca tagacgatgt tctcgagcat gaccgagcgg gttgcagaca cgtcatccgc
    13021 cttgcccaca aggcagttca cccgtcgggg cttgagcgcc tcggcgtagg tcaccgcctt
    13081 gtcgaccccg ttgcggaact cgtcgacccg gtttgtgtcg gcggcaatgc cgcgatcacc
    13141 ggctgcccag tcgccagcgg gcaggttgaa gagcacgagg tcgaggtggt gttcgcggag
    13201 caggtcctga acctcgttgg cccgcatctc gtaggggaag agcatctcaa caccgccgaa
    13261 gccgtttgcg gctgcgcgct cgaaccggtc ggcgagcggg tactcggtaa agagcatggt
    13321 gatattggcg gcaagcttgg gcatatgtgc tccttgtcca tcatgcgtcg cgccaaaggg
    13381 cgcccttgac ggactcattg tgccagttat ccggggcatg gtgcaggcgt cgcgggcgga
    13441 gtttcactgt gtcacactca tccagaatga agttcagcca gttcccccac cgaggacgac
    13501 gatgatactc agggttgaca gcgtgagccg aacattcggg caggtgcagg cctgcaccga
    13561 cgtctcgttc ggcgtggggc gcggagaggt cgtcgcattg ctcggggaga acggcgcagg
    13621 caagtcgacg ctgctttcca tcatcgccgg cttcgttgtg cctgacagcg gtgtggttcg
    13681 ggtggatggc cgtttgttgg agcatgggaa tccgcaggca gcgattcgcg cggggattgg
    13741 cacggcgttt cagcatttct ccctctcgcc cgagctcacc gtcgccgaga gcttcgcgct
    13801 cgctcgcgtc gatgtcgggg aggcgcgccg ccacctgccc gatcacattg atatgggaac
    13861 gcgaacgggc gacctcggag tgccggagca acagcaggtg gagttcgtga aggcgcgcct
    13921 gttgtcagcg cgcgtgatgc tgctcgacga gccgacatcg ctgctcggcg gcactgacgt
    13981 tgaacgagtg ctcgatggta tccaggcggc ggccgatgcg gggatcgcat gcgtcttcgt
    14041 gacacaccgg ctgcgggagg ctctcgcggt ggcggaccgc atcgtggtaa tgcggcgcgg
    14101 ttgggtggtc gagcgcatgg tgcgttcgga cgcgggttgg cccgcagata ccgagcggca
    14161 ggtgctggcg gccatgttcg gcgacgtgaa cgccgtcgat gagccagacg tgccacggac
    14221 ttggcagagc gggccgggcg tcggcacgat ttcggtacag gggcgagtcg gtgaacgaac
    14281 aagggacata tccctccaga taggccaagt gctcgccgtt gcaggaatcg ctggaaacgg
    14341 acaggaggag ctcatcgaac tgctggttgg cacgacggcg gatcgagtcc ggttagagac
    14401 taccggcgca tcgctcgacc tgaccgggca tcggatgcac gagtgggttc ggcacaacgt
    14461 cgccgtggtg cccgaggatc gtcagctcga ggggggcgca cctgcgatgt ccctcggcga
    14521 gaacctggtg ctgcgtgatc tcgccggcgg acgcctctcg acactcggcg cggtcagccg
    14581 acggcgactt cgttcccgcg cacgtggcct gatagaaacc tggtccattc gtcctgccga
    14641 cccgagcgtg cgcttcggat cgctttcggg tggcaacatg cagcgtgcgc tgctggcgcg
    14701 gtcgcttgac ccgctgccga agctgctcgt ggccgtccgt ccggcacacg gacttgatca
    14761 ccgatcagtg gagatggtcc ggcgggagct gcgcgcagcc gctgacgcgg ggacagcggt
    14821 cgtgactgtc gaaggggacc ttgacgaggc gctcgcccat gcggacagta tcgcagtgat
    14881 gtatcaggga cagctctcag ccgccgtccc ggtggcgcat gccgaccgca cggcgctgca
    14941 gtccatgacg gtgagcggat gggacatctg actcgcgata ttgccgtgcc gctgctggct
    15001 gtgctcgggg cggcgctcat aggcgtctcc ctcctggcag cgaccggaca cgacccgctc
    15061 gcggtcggtg aggcgttcgg gcggcgggtg ctgctgcggg gtgcgggcat tgaggagtcg
    15121 gcggtggcga tggggccgtt actcatcgcc gcgctggctg cgtggatagc cgcgcgggtt
    15181 gggctctgga acatcggcat tgacggccag gtgattgccg gggcgatcgc ggccgcgacg
    15241 ctggctccat tcctcgacac ttggcctcgc gagtcgatgt gggttgcggt gacggttgtg
    15301 gcaatcgtgg ccggcgccct gtgggcactg ctcccggcgc tcttgcgtgc gcatgctggc
    15361 ataaacgaga ttgtcacgac catcatgatg acctacgtgg cgttctcgct cggctcgtgg
    15421 ctggtggagg ggccgctccg ggatgaggcg ctggtgactc cggtgaccgt cgcgatcgac
    15481 gtcggtaggc gcttgccgcg cgtgttcgac acccgcatcc atctgggtgt cgccgccgca
    15541 gtggtcatcg tgcttctgac ggcggctgct gcgcgctgga catcgccggg catcctctcg
    15601 cgcctggtgg gtgagtcggc acccgcggct caccggcttg gcttgccggt cgagcgatat
    15661 gtggtcgtcg cgttcctggt gagcggcggg gtggccgcgc tggccggtgt cgccgaagtg
    15721 ctggcgacca gaggcgcggt gcaaggcgac tggcgaccgg tattgacact gcctgcgttc
    15781 gcggccctct ttctggcgcg gcagaacgtg cttgcgctcc tcccggcggc gttcctgctc
    15841 ggcatgctgg cgtatgcatc gacggtcctg ccccgcgcga ccgatctcgc ccccgatttc
    15901 ttcccgatgc tcgaaggcgt gctgctgata ctgcttgcag tggagcgctg gcagcgacca
    15961 gaacgagcgc aagcgccggc ggagcggagc atatgaacgt gctggaaaca ctcctgcagg
    16021 cgtcggtgct cgctgccgtg ccgctgatgc tggctgcggt cggcgaggcg atcggtcagc
    16081 gaagcggact gctcaacctc ggcgtggagg gcgtgatgct ggctggagcg ctggtcgcct
    16141 tcatcgcggt cgagaccagc ggctccttcc ttgccgggct ggcggctggc gcgttggtag
    16201 gtgcggtcgt cgggctgctg ttcggcgtgg cgtcggcgcg aatggcggca tcgcagatcg
    16261 tgctgggact cggaatcgca ctggccgggc agggggcgac cggctttttc tttagagagc
    16321 ggtatggcct gacgcagccg ctgctggagc cggggatgtc gagaccgatc tcggacttgt
    16381 ccgaggtcgc cgttgtcggg ccagcgctct ttgggcacaa gtggttcgtc tacgctgcgt
    16441 gggccggcgt gtttctgatc gccgccacgc tgcgattcac ccgtgccggc ctgctgctga
    16501 cggcgaccgg cgacgcgccg ttcgccacgg aggccgccgg cgtcgacgtg acacgggtga
    16561 gagtcgcggc agcgacggcc gggcaggcac tcgccggtct cggcggagcg gcgctgtcga
    16621 tcgtcgaggt cgggtttttc gctccgggca tgacagtcgg catcgggttc attgcgatcg
    16681 cgctggcgat ggtcgggcgg caggatcctc ttcggattgc gctgctggcg cttgcgttcg
    16741 gtgttcttcg gggactcggg ccggcgattc aactgaccga gtggcgggtg cgcgtggagt
    16801 ttctcgacat gatcccctac attggcgtgc tcgtgctcgt ggtggttctt gggcgacgtg
    16861 ttcggctgcc cgctgcgctc ggcgtcgcgt atcatcgtga agcacgaccg tgacggcgat
    16921 gttcgcaatt ccgcactgac cgagcaggga gtagatggga atgatgagac gcagagcgtc
    16981 cctgatcttg ctgatacttg tgggcttggt tggcccctgg ccgctcggat tggcgcccgc
    17041 agcacagaca gaggttgacc gcgcggcgat agtcacgccg gggagccgat ccaatgaggg
    17101 ctgggaccag cagggtgtcg atgcgctcgt ggccatcggc gaggagcgcg gcatcgaggt
    17161 cgacgtggtc gagaatgccg gctacgatga catcacgccg attctgcggg atcttgctgc
    17221 cgacggggcg gacctcatca tctgccacgc ctccggctac caaacggttt gccccgagtt
    17281 tgccgaaagg gaaggcgtgc cggtggtcgt caccgagaat ccggaggcgg tgaccgaagg
    17341 gcttgtttcc gacgtcgaga cggaggcaca ggaggtggcc tatctcgccg gcgtgatggc
    17401 ggcgctggaa accagcaccg gcacggtcgc ggtggtcgcc tcgggcgagc cgccgacgtg
    17461 gaactatatg acggcaggat tcgccgcagg cgcgaaggac acggatccga gtgttgaggt
    17521 gctatacagc gtgatcggcg aggccgcgta cgatgacgcg cccaacgcgc gccgtaccac
    17581 cgaaaccgtg atcgccgccg gtgctgacgt catcttcggc atgggtgacg gtgcctcgtt
    17641 cggcatgatc caggcaattc gcgagcacaa cgccgcggtg tcccaggacg aggaggttcg
    17701 cttcatcgac gtcatcggcg acaagtccga ctccgatgtg gccgacttcc tgctgacatc
    17761 cgtgctgttc gactacaccg gtgcctacaa caccttcatc gacaacctcg agagcggcga
    17821 cttcggttcc atctacacga tggaccttga gaacgagggg gtttggctgc tggagccacc
    17881 gatcgaagta tcggaggaga ccatcgagca ggtggaggcg gccgaacagg cgatcctgga
    17941 aggcgagatc gacgtgccgg cgatcgcaga ggccgatgag ctggctgact tcctcgaaga
    18001 tctgccggag ggggatgcgt gacccacgaa cctcgcacag attggccaca cctcacgctc
    18061 gcgtccggac cggtcgatgt gccgctgcac accctgcggg caatgcagcg cccaatcgtc
    18121 taccactacg atccggtctt cattgaggag ttccgccggg tcgaggcgct gctgaagcag
    18181 gtgttccgca cggaacacga tgtcatcatc atgcagggtg aggcggtgct tgcgctggag
    18241 gcggctgcgg cgtcggtcat caggcagggc acgaagatcc tcaacctcgt ctccggtgtc
    18301 ttcggggcag cctatgccgg ctggatcgag cggtacggcg gcgagatgat tgagatccgc
    18361 gtgccgtatg atcaggcgat cgatccggaa gaggtccgcc gcgtgttgga cgagcatccc
    18421 gagatcgagg tgctggccgc ggttcattgc gaaacgccct ccggcacgat gaacccgatt
    18481 ggtgagatcg gccgcatcgc gcaggagtac ggcgtgttaa ctattgtcga taccgtctct
    18541 ggcctcggtg cggaggtcta ctcgccggaa gagtcgggga ttgacatcgc cgtcgccgga
    18601 gcgcagaagt gcctcggcgg cgtgcccggg ctctcgctcg tcgctatcag tcctcgcgcc
    18661 tgggaggtca tggagtcagt cgactcgcca ttgcgcggca gctacctgtc gatgctggac
    18721 tggaaggatg cctggatcga cggcgagcgc ttcccctaca ccccgtcagt ggcggacgtc
    18781 cacgcgctgg aatcgacgct ggagttagct gtcgaggagg gcgtggaggc gatggccgtc
    18841 cggcatcaga cgatcgcagc ggcttgtcga gctgcggccc gcgggcttgg gctggagctg
    18901 tggccggcca gcgacgacat cgccgcgccg ggctgtacag cggtgcgggt gccgaagggg
    18961 acgacggctg aagcgatcat cgagacgatg cgttcgcggt acggggtgat gatctccggc
    19021 ggctacggcg atctgagcgg gaagctcttt cgcctcgggc acatgggaca gtcggcgcat
    19081 ccaacgacat tgttcgcgca aatcggtgta ctggagcggt cgctgctcga tctcggggtg
    19141 tccgtcacgc cgggcgccgg cgtcggtgct gcggtcggcg cgctcgcggg ctggggcaac
    19201 tgaccacctc ttgcatgctg cccatcgccc attgattgag gcgatgcctt gtcttgcggc
    19261 tcctatgact cggctagctc gtcgaggcgc tcgcggtcgt cgtcttccgt tccggaccac
    19321 agtcgtcgga agacgacgat ctcgatgacg aagaaaaggg tcgtgatgat cgccgaggcg
    19381 acgcaccact ggcatatcgc ctcgattacg tacagctcga cccaggtcag ccaccctgaa
    19441 tagagggtac cggcggcggt gactgcaagc gccgccgtgc tgccctggag catgagctcc
    19501 ggcttctgca ggcgcaaacc ggcgagggcg agcaggctca ggtacatcag cagaccaaac
    19561 catgcgatcg ggatatccat cacggtcgcg taggaactgg tctggacgat ctcgcagtca
    19621 ccactgccgc agacgagcgc atccaagcca taatgcaccc agaccagata gagctcgatc
    19681 agcacgccga tgccgctgag gatgagggat gcgagaaggt agtccgggtt gtcatcagcg
    19741 ggccccggcg ctaggcgtcg atccgttcgc cggcgagcgc ctggttgatc tgctcctcgg
    19801 gtgtgtcgaa gccgccgcgg gtatacgtga ccagctggtc attgatcagg aaggtcagtg
    19861 tgccgctcac gctgcgctgt agcgcgtcct ccccgaaagc gagcgcttcc tcctccttct
    19921 ggccggaggc aagacactcg ttgaacgcct ccatgtcgag tccgaggtca acggccgcct
    19981 gctccagcat ctcggtggtg tagacgccgg aattctccgc gccctgcgcg gcgaaaagcg
    20041 cctccgagta gggccagaac tcgccctagt cggccgcgca gagggcggct tcggcggact
    20101 gcaccgattc gttttcgtcc gcctcaggcg ggatattctg gaatccgctg acgatcggca
    20161 gcgggcgcca ttcgacccgg acatccccgg tgtcaacgaa ggtgtcgacg acctgtggga
    20221 gatcctgatc gtcgaactgc ctgcagaacg ggcactggaa gtcgctgtag acgacgaagt
    20281 caaccggggc gtctggatca ccgaggacgc ggccatcgtt ctcgacctct tcgagcgcca
    20341 gtgcagcctc cggtgcctcg gtctcgtcgt cctggttggc gaggacaagc gccacgaccg
    20401 ccacgagggc gatcgccagc gtgccggcct ccaggagcag catgcgtcgc tgtcgctcgc
    20461 gcttctcgcg gcggcgcgac gcgccacccg gcgctgggac ttcgtctgtc ggcggttcgt
    20521 gggttccggc atggcgcact caacagtgac gatacggtgg ttttccactg cgataatggt
    20581 gggacacgat tggccagcga ctccatctca gccagtgccg aagactgccg cattgccgca
    20641 gtgcgccttt caggggtttg ggagagcggc gaaacttcgt acaattcagc acgagcaaga
    20701 gccgcttcgg ttccgatggt cgcgtgagga gtgatcacca tgacggatca ggacaacgcc
    20761 cggcgttcgg tccggagcgc gaaattggaa cgtgtgctgg tgccggtgga cggttcgccg
    20821 gcgtcggaac gggccctgga gtacgctgct cacctcaggc cggagcgcat cgtactgctg
    20881 cgggtcgagg tggacgagcc cgtgggcccg catgtcgacc cggctgatcc ctacgagcgg
    20941 tggcggcgcg accatctcag cacggtaatg aacgagctcg agtcgctggc gcgctccaag
    21001 gcagcgacgg ccgatgtggt agagccaaag atccggttcg ggaaccccgc ggaccggatc
    21061 atggcggaag cccacgatca cgatctcatc gtgatgggcg caaccggcaa gggcgcggcc
    21121 ggccggctgt tgttcggtag cgttgccgac cgtgtgatgc ggaacgccat cacacccacg
    21181 ctggtcgtgc ggacgaacgg agacgaggcc gaaattcggc ctcccgcgcg ggtggtagta
    21241 ccgctggacg gctctgagct cgccgagcga gcgctcccaa tggcgacgcg agtgtcggcg
    21301 gcgctttcga tcccgatacg gttggtgcgc tgtgtgggaa tggacgaggt gcttcacacc
    21361 gttcgtgaag cgcgacgatc tggcgatgcc gagctgtttg aatcgagcga cgacccctac
    21421 aagctcggac aggaacgcgc caaccgggag gctgaagcgt atctcgaagc ggtaccggag
    21481 cggctgggtg tcgcgggaca gaacgtcacc tgtgaacggc tgagcggttc tgcggcattc
    21541 gagttgctgt ggagcctcca ggaggatgaa ctggtggtga tgacctcgcg cggtcgggga
    21601 gggctgcagc gctgggtggt cggcagtgtg gccgagaagc tggtgcgcga ggcgaaagcg
    21661 ccggtgctgc tcgttccagt tgggcgagcc ggcatggagt ccgagtagcg gagcagtacc
    21721 tgatcttgtg atgtggtgtc catcggaggt gctcaaagtc ctgatgcatg ggcgtccacc
    21781 atgccggctc gcttcggcac gcaaagcggc tcgtcaccca gtggcccggg cctggtctct
    21841 gcgagaggtc ggaaaccgtc tgcgatggat gatgaggatg gaatcggcac gaagcgggag
    21901 cggcatatgc cgc
//
LOCUS       DASYWL010000227         5793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_367127, whole genome shotgun sequence.
ACCESSION   DASYWL010000227 DASYWL010000000
VERSION     DASYWL010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5793
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367127"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1429
                     /locus_tag="VGR22_08070"
     CDS             <1..1429
                     /locus_tag="VGR22_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128558.1"
                     /translation="ELPLTSLQYGGCFCKDCMKGFRAYLQALPIEALPDALRGADLAS
                     FHYGEWLRLQRDEIRGLDGDTPLFEQYLRFQRQAVARYFGELADYVHEYGASKGREVA
                     VSGNLFNLFDHFHAVESKVDLIITEMRNTRYRQPGWYRYVAGFAGPKPTVVVENPYGG
                     VVPELVEKLNRGEGYNLFRLSLYEAAAMGVNMSVPYGAWLGSSAQDAFYAPHDLSVEI
                     QQFLANHQHLYSRETCNEVAVVYSVECNFLESARMNFFADNRQGVPRGEALPFWQVCE
                     TLADTAQPFDVVFFPDTRLRPDELTLEDLRQYRTIVLPDCRSLTAAQYELIRDFLADG
                     CCIVVIGDFGLNLPDAVRHGVLGHERTTLVRRVDGMSVEHLSSGPQVRVVEPAALGVN
                     LQRVAEGVAVHIVRYDFDERQDRIPDLATLTMELRLPGQWRVYGAYGTGGTIAASLST
                     AGEMHRLELENVPLYGIVSLREARI"
     gene            1730..1921
                     /locus_tag="VGR22_08075"
     CDS             1730..1921
                     /locus_tag="VGR22_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128559.1"
                     /translation="MLSFLRIEWPDGFITENQLRDWIHHPWHHSTHFVLVEGEMLVSH
                     VSVLWKDLEHVGTVYRRMS"
     gene            1927..2352
                     /locus_tag="VGR22_08080"
     CDS             1927..2352
                     /locus_tag="VGR22_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128560.1"
                     /translation="MLTYLAFRRQGFGSRLVSAATAFIRDGDADVGLFTCQPHLRDYY
                     AAHGWIPIERAVLLGGPRTDPSPSDGVVMMGFFSEKGRQGREAFTSHSILFDDDPWST
                     LRLSDSYCLVPPPCCNSLKRESGSLGRLSRDFSPRAIAA"
     gene            complement(2511..3488)
                     /locus_tag="VGR22_08085"
     CDS             complement(2511..3488)
                     /locus_tag="VGR22_08085"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV2128561.1"
                     /translation="MVELSIAVEGPYGLSWPEWKRWVRTVEEAGFAGLYMPDHFVLPE
                     PPDADYPSLELVVALTYLADHTERIRFGPMVAPLSVRDPVMLARQAAALDALSEGRMV
                     LGVGAGWMEREHAMFGYELGDVPTRFARFEEGLEVVSRLVRAGEPVSFEGRFFHLQDA
                     ALPPPRRPGGPPIMIGGSGPRRTLPLVARFADVWNAQLVSPEQLRERSTLLDELLEAA
                     GRRSQDVRRTLAVLVLCWRTPEELEARIRGLRRLPGWQGLSAEQIVAELRAWPAAIIG
                     TPEEVVSRIDAYAAAGVSEIALQWFAPEDIDGLEVLAEEVLPLLASAGS"
     gene            complement(3626..4438)
                     /locus_tag="VGR22_08090"
     CDS             complement(3626..4438)
                     /locus_tag="VGR22_08090"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEV2128562.1"
                     /translation="MDLRLMRHLWGVSESWEQAFPRFKAAGYHGIDALPPEPQDIARF
                     RQLLKDYRFDYILQVLTTGATVEEHVASFRQQVEAGQVLEPLLIGCHSGRDAWSERES
                     AEFFVQALAIEREIGIPVGHETHRGRILYNPWVTVLMLDRFPDLKLVGDFSHWTCVAE
                     RLLGDQADIIARCAERCLHIHARVGYEEGPQVPDPRAPEYRYCLEAFERWWDLVWDAQ
                     AARGLAVTTLTPEYGPPGYLHTLPYTRQPVADAGEISDWQAQREAARFAMRE"
     gene            4704..5375
                     /locus_tag="VGR22_08095"
     CDS             4704..5375
                     /locus_tag="VGR22_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEV2128563.1"
                     /translation="MATTRSTPVGTTPCRPDSRWPLGIPASSRHGRPSQGQRARDVTA
                     YLAADLDPKLATIIAAAARHEPATGPGTEEDGHALTGPVFVVGAEPGDTLVVEIVDVR
                     PADWGWTTCGPGVGLLGDAFEEWTLHVWDLRSGTTAVFAPGITVPMAPFCGVMGVAPG
                     KPGHHDLTPPRRAGGNMDVRQLTAGATLALPVLVPGASFRWATPMPPKGMARWPGRVS
                     RRTPP"
BASE COUNT         1037 a         1884 c         1865 g         1007 t
ORIGIN      
        1 ggagctgccg ctgaccagcc tgcagtacgg cgggtgcttc tgcaaggact gcatgaaggg
       61 cttccgagca tacctgcagg cgctcccgat cgaggcgcta ccggacgcgc tgcggggcgc
      121 cgacctggcg agcttccact atggggagtg gctgaggcta caacgtgacg agatacgggg
      181 gctggatggt gacacgcccc tgttcgagca atatctccga ttccaacgac aggcagttgc
      241 tcgatatttc ggggagctgg cggattacgt ccatgagtat ggcgcgagca agggacgcga
      301 ggtcgctgtc tccggcaatc ttttcaacct cttcgaccat ttccacgcgg tggaatcgaa
      361 ggtcgacctg atcatcaccg agatgcgcaa cacgcggtac cggcagcccg gctggtatcg
      421 ctacgtggcc ggatttgctg gccccaaacc gacggtcgtc gtggagaacc cgtacggcgg
      481 tgttgtgccg gagctcgtgg agaagctgaa ccgtggggag ggctacaacc tcttccgtct
      541 ctcgctctac gaagcggcgg ccatgggggt caacatgagt gtgccctacg gtgcgtggct
      601 gggcagctcc gcacaggacg cgttctacgc accacatgac ctttcggtcg agatccagca
      661 gttcctcgcg aaccaccaac acctctacag tcgcgaaacc tgcaatgagg tggccgtcgt
      721 ctacagcgtc gagtgcaact tcctggaaag cgcccgcatg aacttcttcg ctgacaatcg
      781 gcaaggtgtg ccgcgtgggg aagcactgcc attctggcag gtatgcgaga cgctggctga
      841 taccgcacag ccgttcgacg tggtcttctt cccggacacc aggcttcgcc ccgatgagtt
      901 gacgctcgag gacctgcggc aataccgcac gattgtcctc cccgattgtc gctccctgac
      961 ggctgcccag tacgagttga tccgtgactt cctcgcggac gggtgctgca tcgttgtcat
     1021 tggtgatttt gggctgaatc tcccggacgc cgtacggcac ggcgtcctag gtcacgaacg
     1081 gacgactctt gtccgtcgtg tcgatgggat gagcgtcgaa cacctttcga gcgggccgca
     1141 ggttcgcgtc gtggagcctg cagcgctagg agtcaacctt cagcgggtcg ctgagggcgt
     1201 ggccgtccat atcgtccggt acgacttcga cgagcggcag gatcgcattc ccgatttggc
     1261 gaccctgacc atggagctgc gactccctgg gcagtggagg gtctacggtg cctatggaac
     1321 gggaggaacg attgcagcct cattgtcgac ggcgggggag atgcatcgcc tggaactcga
     1381 gaacgtcccg ttgtacggca tcgtatcgct aagggaagcg cgaatataga gcggatcgga
     1441 ctcgagcttg aggccgcagg ggtaatcgac ctggccgggc tgctgacgca ccggctcggg
     1501 ctcgacgaga tcgtcctggt gttcgcggcg ctgcccgaca agccaccagg gttcaccatg
     1561 gcagtggtcc ggccatagcg acacagatta gcgacagtga tggtcaccaa caccgactct
     1621 cgctggttca gcggctcatg gcggtatcgg caaacctgaa ggggtatcaa gatgccggag
     1681 atgacgagtt acacgggcga cgacttgccc gagcatctta aagtgccaag tgctgtcgtt
     1741 tctgcggatc gaatggcccg acgggttcat cactgagaac cagctgcggg actggatcca
     1801 ccacccttgg catcactcca ctcacttcgt gctcgtcgag ggcgagatgc tcgtcagcca
     1861 tgtgagcgtc ctctggaagg atctggagca cgtgggaacg gtgtaccgac gtatgagctg
     1921 agcggcatgc tgacctatct cgcgttccgc cgacagggct tcggcagccg gctcgtgtcc
     1981 gcggcgacag ccttcatccg agacggcgat gcggacgtcg gcctgttcac gtgccaaccc
     2041 catctgcgcg actactatgc cgcgcacgga tggattccaa tcgagcgggc cgtcctgctc
     2101 ggtggtccga gaaccgatcc ctcgccgagc gatggcgtgg tgatgatggg cttcttctcg
     2161 gagaagggca ggcagggtcg cgaggccttc acatcccact ccatcctgtt cgacgacgac
     2221 ccatggtcaa cactgaggct cagtgattcg tactgcctcg ttcctcctcc ctgctgcaat
     2281 tctctgaagc gggaaagcgg aagtctcggg cggttatcaa gggatttttc tccaagggcg
     2341 attgctgcat gacaagcaac aaagatgcgc tctcttctcc gcgctatact gcctccgcaa
     2401 gggggatatg ccacagatag aagtcatcaa agcgtcggaa gcaccggcac cacccaagaa
     2461 aacgaaggcg tctagccaga cctagggaga ttgggaaggc tcttctctgc tcacgagccg
     2521 gcagacgcga gcaatggcag cacctcttcg gccagcacct ccaacccgtc gatgtcctcg
     2581 ggcgcgaacc actgcagcgc gatctcgctg acgccagcag ctgcgtacgc gtcgatccgc
     2641 gagacgacct cttccggcgt cccgattatt gctgctggcc aggcacgcag ctccgccaca
     2701 atttgttcgg ctgacaggcc ctgccaccca gggagccgtc gcagtccacg gatccgcgcc
     2761 tccagctcct cgggcgtgcg ccagcagaga accagcaccg ccagtgtccg gcgaacgtct
     2821 tgcgacctgc gtcccgccgc ctccagcagc tcgtccagca gggtcgagcg ctcccgcagc
     2881 tgctctgggc tgacgagctg cgcattccag acgtcggcga agcgcgccac gaggggcaat
     2941 gtccgccggg gaccgctgcc gccaatcatg atcggcgggc cgcccgggcg ccggggcggg
     3001 ggcagcgccg catcctgcag atggaagaat cgtccctcga agctgacggg ttcaccagcg
     3061 cgaaccagcc gcgagacgac ctccaacccc tcctcaaagc gggcaaagcg tgttggaaca
     3121 tcgcccagct cgtagccgaa catggcgtgc tcacgctcca tccagccggc gccaacgccg
     3181 aggaccatgc ggccctcgct cagggcatcg agggcggccg cctgccgggc cagcatcaca
     3241 gggtcgcgga ccgagagcgg ggcgaccatc gggccgaagc ggatccgctc ggtgtgatcg
     3301 gccaggtagg tcagggcgac caccagctcc agcgacgggt aatcagcgtc gggcggctcg
     3361 ggcaggacga aatgatcagg catgtacagc ccggcaaagc cggcctcctc gaccgtccgc
     3421 acccaacgct tccactccgg ccagctcagg ccgtacggtc cctcgaccgc gattgacagc
     3481 tcgaccacga tgtagctcct cgccgaccgt ttgcggttac gtcttccggc tgccgccggg
     3541 ctgctccgga acccaaccat cgcggcaacg gaaaccacag aacgaaatag ccagtgcgtt
     3601 gaatactggt cgcttcatgc cgtgtctact cacgcatcgc aaagcgcgcg gcctcccgtt
     3661 gtgcctgcca gtcgctgatc tcccccgcat ccgccacggg ctggcgggtg tagggcaggg
     3721 tatgaaggta gcctggcggg ccgtactcgg gggtcagcgt ggtgaccgcc agcccacgcg
     3781 ccgcctgcgc gtcccagact aggtcccacc atcgctcgaa cgcctcaagg caataccggt
     3841 actccggggc ccggggatcg ggaacctgcg gcccctcctc gtatccgacg cgggcgtgga
     3901 tgtgaagaca gcgctcggcg caccgggcga tgatgtcggc ctggtccccg agcaggcgct
     3961 cggccacaca ggtccagtgg ctgaagtccc ccaccagctt gaggtcggga aagcggtcca
     4021 gcatcaggac cgtgacccac gggttgtaga ggatgcggcc ccgatgcgtc tcgtgcccga
     4081 ctgggatgcc gatctcccgc tcgatcgcga gcgcctgcac gaagaactcc gcgctctccc
     4141 gctcgctcca tgcgtctcgg ccgctgtggc agccaatcag tagtggttcg agcacctgcc
     4201 cggcctcaac ctgctggcgg aacgacgcga cgtgctcttc aaccgtggcg ccggtggtca
     4261 ggacctggag gatgtagtcg aacctatagt ctttgagcaa ctgccgaaac cgggcgatgt
     4321 cctgcggctc gggcggcagg gcatcgatcc cgtggtaacc ggcggccttg aaccggggga
     4381 aggcctgctc ccaggactcg ctcacgcccc acaggtggcg catcaaccgc agatccatcg
     4441 gtaccctctc catcgtgcgg accgattcta tcacgcagag tcctcgacgg tggcttatgg
     4501 ggcactcgat gccatactgc tcgtgagtcc gtcgaggact cacgcctttg tgtctgcggc
     4561 ctttgaatgg cgcgacaggg cccgcagacc tcgaacgctt ggtgccgtgt attgatctat
     4621 cagcccgctg ggcccatcga gggtgacgag caatgagggg gcaacgagga tagagatggg
     4681 caacattgga cagacgcacc accttggcca cgacgcgatc cacaccagtt ggaacaacgc
     4741 cttgccgccc cgactcacgc tggcccctgg gaatacctgc gtcttcgaga cacgggaggc
     4801 cctcccaagg ccagagggcc cgcgatgtca cggcctatct tgcggccgat ctggatccca
     4861 agctggccac catcatcgct gccgccgctc gccacgagcc agcgaccggg ccgggtaccg
     4921 aggaagatgg ccacgccctg accgggccgg tctttgtcgt cggcgccgag ccgggcgata
     4981 cgctggtggt cgagatcgtc gacgtccgtc ccgccgactg gggctggacg acctgcgggc
     5041 ccggagtggg cctgctcggc gatgccttcg aggagtggac gctgcatgtg tgggacctgc
     5101 gatcggggac cacggcggtc ttcgcacccg ggatcaccgt gccgatggct ccgttctgcg
     5161 gggtgatggg tgtggcgcct ggcaaaccag gacaccacga cttgacgccg ccacgccggg
     5221 cgggcgggaa catggacgtg cgtcagctca ccgccggggc cacgctcgcc ctgccggtat
     5281 tggtgccggg cgcctcattc cggtgggcga cgcccatgcc gcccaagggg atggcgaggt
     5341 ggccgggacg ggtatcgaga cggacgccac cgtgatgctg cgcttcgacc tcatcaaggg
     5401 aagcgccgtc cccgggccgc aggtccacac gcccgtcgtc aaccaatggc agggccctgg
     5461 ttagcggcga ccggccacca cgccgacctg tacgaggcgc gcgcgaggcg gtccgcggca
     5521 ttctgagcta ccccgaggac caccacgagc tggatcgggc gcagggcgtg atcctcacca
     5581 gcgcctgcat cgacctgcgc atcagccaga tcgtcgtgca atcgatcgct gcccaatgct
     5641 tctcaatttc cccgtactag agcgggttgc agggtgattg acgacttggc agagtcggta
     5701 gatgccacga gggtattccc gcgacctccg tgagcgactg ctgcaggccg cggcgtcggg
     5761 cctgtcggtg gttgaggttg cccgcatcac tgg
//
LOCUS       DASYWL010000228        22465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_48112, whole genome shotgun sequence.
ACCESSION   DASYWL010000228 DASYWL010000000
VERSION     DASYWL010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 22465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22465
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48112"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VGR22_08100"
     CDS             complement(<1..402)
                     /locus_tag="VGR22_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV2128564.1"
                     /translation="MPAFELASSLQPTGDQPKAIEQLTESAQGGERFQTLLGVTGSGK
                     TFTMANVIQNLNRPTLVLAHNKTLAAQLYAEFKEFFPRNAIEYFVSYYDYYQPEAYVP
                     RTDTFIEKDADINEEIDKLRHAATRSLLTRRD"
     gene            complement(405..1061)
                     /locus_tag="VGR22_08105"
     CDS             complement(405..1061)
                     /locus_tag="VGR22_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128565.1"
                     /translation="MTDFASDIVDVYVFRRLNARVQFLLLQRHNDVPMAQTWQAFHHQ
                     IADGQTAPEAARAALKSLANLEIDRFYSADFVNQFYDEERDVVVLAPVLAVTINDQSA
                     VTLADDFQDAGWFDRDEATSRLPFSGQRWAVRHIDEIMGIGESEAELYRFEPTAQPAK
                     QETIRTDTTPSTSTSDTLGDDLGLEPPPEADATDEHAEVDSPDTQTTPTDISQEWRQD
                     "
     gene            complement(1089..1556)
                     /locus_tag="VGR22_08110"
     CDS             complement(1089..1556)
                     /locus_tag="VGR22_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128566.1"
                     /translation="MSSTDSPEQPQSRLDRELNEILEQSRNRPISFHDHVARKRNAME
                     ARRHSAAHRLCQLKSGPVRVVGHWALRIPLVTALVLALIAVWLAPEYQFAASLLALGA
                     AAMIFVPFFLRRPDTDSMHQNRWRGRIISSSPPVGRGRGARSWLDSARDRFGR"
     gene            1688..2161
                     /locus_tag="VGR22_08115"
     CDS             1688..2161
                     /locus_tag="VGR22_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128567.1"
                     /translation="MGNYRFNVIDEEGTASFLGPRHGSKMIAAACGANHRSLSSLLTY
                     IRALDDVWATEVESGLRAFDERNGETEAETPMPDPESGWKAPFRVVDSETRRQSMKIS
                     SLGLFVVNLKARRIVQVDNRSHSLEKSGKGRVRRNGRPTRMLFRYDLPAEWDLVP"
     gene            complement(2293..3303)
                     /locus_tag="VGR22_08120"
     CDS             complement(2293..3303)
                     /locus_tag="VGR22_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEV2128568.1"
                     /translation="MAQNVTIIGLGLIGGSIGLALKRWSRENSNALRITGFDEQISQQ
                     NKAKKMGAIDGTEWTLGKAVSDADVVIVATPVQTMREVFSDIAEFLKPGAIVTDTGST
                     KADVLEWAKELPSTVSFVGGHPMAGKSEGLDGADADLFRGATWVICPSVSASEDAIRN
                     VLGIVAATGADSYFVDPTEHDSYVAAVSHLPFVTAISLVNAVTSDPAWRDMRNLASTG
                     LKDSTRLALGSPEMHRDIAMTNRDAIVLWIDRLTGELQTVRRELVANDENLADTLFKR
                     FEQAQDARARLEVPVSRSDEAMTESQRSVSDEGVGEQMQRMFFGGFRRRGREGEKSNG
                     KR"
     gene            complement(3344..3925)
                     /locus_tag="VGR22_08125"
     CDS             complement(3344..3925)
                     /locus_tag="VGR22_08125"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEV2128569.1"
                     /translation="MSDPGLSYRLRQRSRRAGFMIGLSMLLTIALCAVTFTMIYSALE
                     SFTNDFIPEGDETQPTVAQLAQEPNPGETDERIEQVVAEPNSGDANSTQTSNPTPTSA
                     AEAESQDSEDQDTDEGFNPDYQLRGVTVNLRSGPSTSTDIVTALPPSTPLEYLNEDAP
                     TESPSDGERWMQFVTEEGLEGWIRAIDVTEYEP"
     gene            4297..5658
                     /gene="nusA"
                     /locus_tag="VGR22_08130"
     CDS             4297..5658
                     /gene="nusA"
                     /locus_tag="VGR22_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEV2128570.1"
                     /translation="MKSDLYTAIAQIAAERGIPREAVLSSVQEALTSVYKKSANSEED
                     VRVELDSATGEMQVVVVKTVVENVENPDLEMAVEEAQQYVPGAQPGDVVRIQRTPENF
                     GRIAAQTVKQVVHSRIRDFERENIYREYQDKEGEVLNGVVQRADSRAVIIELGKAEAV
                     MPAREQVPSERYRTNQRVKVLLTEVNSHSRGPQLIVSRSDPRLIRRLFEQEVPEIQSG
                     AVEIMEIAREPGLRSKVAVAARQEKVDPVGSCVGVRGVRIQNIVNELYGEKIDVIEWS
                     PDMATFIANALSPAKPTSVSLGEEGDQKVATVIVPTDQMSLAIGKEGQNARLSFKLTG
                     WRIDIKGPESLLEAGEDFFRSARESAGEMMPGFGWQGRQPRAVQVDGTFTVAGKTYGP
                     LDEELARRQVDVDVKDGVVEVYYERDLRARFDQETGEELPVDGDDAFEAAWKAAQPAV
                     DSD"
     gene            5679..6032
                     /locus_tag="VGR22_08135"
     CDS             5679..6032
                     /locus_tag="VGR22_08135"
                     /inference="COORDINATES: protein motif:HMM:NF016204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEV2128571.1"
                     /translation="MNQPQARAQARKRQKGPRPQHVPQRTCVACREKGDKRGLIRIVR
                     SPEGEIAIDPTGKRNGRGAYLCNKPGCWRKALDTPILAGALNADIPEATERELQEYAA
                     ALEDGEASKGSAAAL"
     gene            6045..7937
                     /gene="infB"
                     /locus_tag="VGR22_08140"
     CDS             6045..7937
                     /gene="infB"
                     /locus_tag="VGR22_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEV2128572.1"
                     /translation="MADTRRRPNRVSRSGGGRRGRRSGSGSARTVAVKRAAPAEREPV
                     VLPAVMSVSELADRLEMSGIEVIRELMKLNIMASLNQQIDYDTAAKVATELGWETLEE
                     ESGTRSAVADFEARRLESEQDPDARARPPVITIMGHVDHGKTKLLDTIRSTKVAEGEA
                     GGITQHIGAYQVETQGRKLTFLDTPGHQAFTAMRARGAQATDIAVLVVAADDGVMPTT
                     REAIAHIKSANVPTVVAVNKIDLPAANPDRVKQQLSELEVMPEEWGGDIPFVEVSALE
                     GLGIDDLLEVLLLVADVNELKANPDKIATGVVVEARVESSRGPVTTLLVQSGTLNLRD
                     VIVAGSTWGRIKAMFDDRGKRIRRAGPSAPVEVLGLMEVPEAGDTFMAFEDEKAARQL
                     AEQRQAQRRVESLTENRPLKLTELYDQVQEGTTAELRVIVKADVQGSLGAIQGSLLKL
                     NEETDAGVTLSIQYASTGAISESDINLASTTNSIVIGFNVRPDAAAKRAADVAGVDVR
                     YYTIIYQLIDEVKQAMTGLLAPVFEDVTDGYAEVRETFKLPNNDVVAGLYVLDGRINR
                     NSRARVLRSGTVVFEGGLSSLKRFKDDVREVSAGYECGLGLEGFNDIQVGDQIEFFHR
                     EEVART"
     gene            7995..8393
                     /gene="rbfA"
                     /locus_tag="VGR22_08145"
     CDS             7995..8393
                     /gene="rbfA"
                     /locus_tag="VGR22_08145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEV2128573.1"
                     /translation="MTRRTRQVGELLKEELTDIIRKDVKDPRIGFMSITEVDVTPDLR
                     QARVYVSVLHEEEREGTMDALRSAASFIRHHLKPRLRMRQIPFLEFRSDRSMEYAQRI
                     TETLREIERQDAARASREPATEASSEQEHS"
     gene            8390..9400
                     /locus_tag="VGR22_08150"
     CDS             8390..9400
                     /locus_tag="VGR22_08150"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEV2128574.1"
                     /translation="MTGALWRLDRQAAEDALELLRGASRVVAPTHQNVDADGLSSAIA
                     LRLGLAQLGVELVPVVSDGQLPRSLRFLPGIDEVLVYGKDDLPDYDVLCLVDASDKRR
                     LGDFYGDDPERVSGNDVPIVNIDHHVTNDRYGKANIVEPSAAAAAEIVADLLMEWGTE
                     LNRDIAQNLLAGIYGDTLGLRTDSTTSRTLRTSADLVDAGGDPSQIVDALFRIKPRSS
                     VCLWEKALRNVQYTGGLIWTELTKETLDRCGADASEAEGMVNFLVGTEGTHAAALLYA
                     NDKGWRVSLRSLPSGVDVAELAAEFGGGGHPRAAGVQIEGGEEEKRAFVEVVGRLAQE
                     HA"
     gene            9406..10317
                     /gene="truB"
                     /locus_tag="VGR22_08155"
     CDS             9406..10317
                     /gene="truB"
                     /locus_tag="VGR22_08155"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEV2128575.1"
                     /translation="MARKRTPPHQSGFLVIDKPAKWTSHDVVGRVRRVMGERQVGHTG
                     TLDPAATGVLPVAVGHATKLLPFIEDTDKTYIATVRFGVVTDSGDRDGRLLAQPAADH
                     VTEAGVRAALDRFRGEIGQVPPMHSAIKVGGRKLYDVARSGGDIDVPVRRVYVHDIEL
                     LEWEHPAATIRVSCSAGTYIRTIARDLGEAVGSGAMLSRLTRTKAGMFGLRQALSIHD
                     LEASLDCWGWPMVSLHPDAVLPDADLLILDEGEERRWFNGLPIERLAMTPVVRVYDSR
                     REWTGIGLRDDDGVRIHPKRVVRARSE"
     gene            10320..11255
                     /gene="ribF"
                     /locus_tag="VGR22_08160"
     CDS             10320..11255
                     /gene="ribF"
                     /locus_tag="VGR22_08160"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HEV2128576.1"
                     /translation="MTADPLELLRQPVGRLRVATIGTFDGVHRGHQALIEYAASRARE
                     AKVPLTVITFDPVPAAVLRPERFPGAIAPLGEKLRQLEAAGAGEIIVLPFTPELSCVT
                     AEAFMDVLANDARVAELYTGEGFALGHKRQGTVDVLNHIAAKRGMRFEAIPRIEDLHG
                     VVASSEIRRAIQRGDATLAASLLGRWFRAEGEVVRGAQVGRQIGYPTANMLPAEDMVQ
                     LADGIYATLSWLPGEEHARHSMTYIGTRPALNTGRRFIETHVLDFDGDLYGRILSVDF
                     VKRLRPDADFPTVDELIAQLRRDEAEARQVLARTS"
     gene            11436..11951
                     /gene="infC"
                     /locus_tag="VGR22_08165"
     CDS             11436..11951
                     /gene="infC"
                     /locus_tag="VGR22_08165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEV2128577.1"
                     /translation="MNDRIRVREVRLIDEEGQQIGIIPTREALMIARERGLDLVEVGA
                     NANPPVCRLMDYGKYRYEQSRRDRENRKSQHVTKVKEVRVEPKIGEHDLETKGRQAER
                     FLKSGDKVKLSVLFRGRSITHPELGRDLLQKLADQLGEVGVVEQGARMEGRSMTMYMA
                     PTRSKGDDKDK"
     gene            11968..12180
                     /gene="rpmI"
                     /locus_tag="VGR22_08170"
     CDS             11968..12180
                     /gene="rpmI"
                     /locus_tag="VGR22_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEV2128578.1"
                     /translation="MANRGKLKTHKGAKRRFKITGTGKIMQAKGMKSHRRRKKAPRVK
                     RQFDKMFEMHESNEKRVRRMLPNGLK"
     gene            12217..12582
                     /gene="rplT"
                     /locus_tag="VGR22_08175"
     CDS             12217..12582
                     /gene="rplT"
                     /locus_tag="VGR22_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEV2128579.1"
                     /translation="MARIKRGVTSHRRHRKVLQQVKGHRGTNNRLYKRAHESLMRSLA
                     YQYRDRRNRKRDMRRLWVQRINAASRANGLQYSRLIHGLSLAGIEIDRKMLADLAVRD
                     EEAFTAVVDAARQALPAQA"
     gene            12661..13407
                     /locus_tag="VGR22_08180"
     CDS             12661..13407
                     /locus_tag="VGR22_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEV2128580.1"
                     /translation="MRSLERRRYRQAERAFLVEGRRLAEEALRMAPDTVRLLLVREDV
                     PSGWWREFGLDASKVRRVDRKVFEAASGVEHAQGVAAVCDLPVPAGIDDLRRAVGPVL
                     VLDRLRDPGNVGTALRSAAAAGAEIVLTTPATTDSWSPKVVRAGMGAHFRLRIGAFGE
                     DAASFLRETIHDVVCADASALEPYHARDWRSAAALIIGGETEPLSAELETLETHRVRI
                     PMRGGMESLNAGVAASVLLFEAQRQGAYER"
     gene            13557..13853
                     /locus_tag="VGR22_08185"
     CDS             13557..13853
                     /locus_tag="VGR22_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEV2128581.1"
                     /translation="MRKQDLIKAVAEELGRSESEAGNAVNAVFSVIERSLANGRDVTV
                     SGFGSFKVVERSAREGRNPQTGEAMTIAAKKAPVFRAGTQLKRSVESAQGATLG"
     gene            complement(14100..15137)
                     /gene="plsX"
                     /locus_tag="VGR22_08190"
     CDS             complement(14100..15137)
                     /gene="plsX"
                     /locus_tag="VGR22_08190"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEV2128582.1"
                     /translation="MPTEPAIIAVDAMGGDHGPTVVVGGAVAAARRFSVGIRLYGDKS
                     ALDIALGQHDLAGLHVEPVPTVDTIGMDEHPTQAVRRKPESSLMRSIRAVQDGEAGAM
                     VSAGNSGAVMAGALMNLGRVKGIDRPAIASHMPSKAGRALVLDLGAVTDPKPAHMVQF
                     AIMGQTYARYVLSVPEPTIGLLSNGEEGSKGNQLTVRVHELLQQTPGIDFHGNVEGKD
                     IMLGTTDIVVADGFTGNIALKTAEGTAAFLLDALKSELRASPFRKLGAAMLKPAFREV
                     ARRLDYAEIGGAPLLGVDGNTIIAHGRSNALAVENAIGVALRSIEQQLPSRIAERVSE
                     ISEQAQTAQEA"
     gene            complement(15168..17114)
                     /gene="gyrB"
                     /locus_tag="VGR22_08195"
     CDS             complement(15168..17114)
                     /gene="gyrB"
                     /locus_tag="VGR22_08195"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012535740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEV2128583.1"
                     /translation="MATQVKEALNETAYDASQIQVLEGLEGVRRRPGMYIGDTDQRGL
                     HHLIREIVDNSVDEALAGHADHIVCTIGADGVITVVDNGRGIPVDTHTKSGKSALELI
                     MTHLHAGGKFGGGGYKVSGGLHGVGASVVNALSEWTTVEVRKKGDPNVYKQEYSRGKP
                     TTKVQVIGKDTKSSHGTTTHFLADTQIFMSGLNYQFDAIAQWFRETAYLTRGLKLTLK
                     DERTDKEMSFYFEGGINSFVRHLNRSKQPLHDRPFSVHREIETPQGVVDVEVALQYNN
                     SFGETTYTYANNIHTIEGGTHFTGFKTALTRTINDYARRNGFLKNNDDSLSGDDVREG
                     LTAIVSVKIEDPQFEGQTKGKLGNAEVAGAVQSVVNESFGAFLEENPQVSRRVIEKCL
                     NAARAREAARKARELVQRKGGLDNFSLPGKLADCSERDPSRSELYIVEGDSAGGSAKQ
                     GRERRFQAILPLRGKILNVEKARLDKMLQNNEIRTIITALGTSVGEQFDISKLRYHRI
                     IIMTDADVDGAHIRTLLLTFFYRHLEPLIRDGFVYIAQPPLYKVGRGNSEEWVFSDRE
                     LEQVLKDRGENREVQRYKGLGEMNPEQLWETTMDPTKRTLKQVTIEDGQAADETFDML
                     MGAAVPPRRKYIQAHAQDVQNLDI"
     gene            complement(17173..18165)
                     /locus_tag="VGR22_08200"
     CDS             complement(17173..18165)
                     /locus_tag="VGR22_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128584.1"
                     /translation="MTETVASGLSVPLSRPWLLVLPILLDVLLLVGMQIPITRLITPL
                     ADEMEAIGGENGDLAAEQFREVGETFWLNDVMGSLLPSVFAGLSHDNLFNVMMAVFTP
                     GLADGVDRGDLADTWLAATGAVRDPGSIWSILGLGLLFFLVSTLLTVGWRVPLALLVA
                     GRTMRPIEVARYVARSWLRFLGLIGLMIVGAMVVIAPVMIGAGVLLLMQVNLAALISL
                     FLVLLGSLIAIYTRFVLESIVVNDIGPLRALRRSARLAHHFFGPTVRFSIAVMLIAVG
                     ALRLWDTMASSVPGLPIAILVNSFLGTGIAIATMMFYYDRDRIIRKFETAPGSK"
     gene            complement(18228..18638)
                     /locus_tag="VGR22_08205"
     CDS             complement(18228..18638)
                     /locus_tag="VGR22_08205"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEV2128585.1"
                     /translation="MKTGKIEGQRRVAALRAKARRLQFSRRVDAALRYLPEQYRQYLD
                     NVAIVVEDEPPAASPAGESEELFGLYHGIPRSQRYGGYSMVVPDQITVFRGPLTRAFG
                     IGPELDEEIRITVLHELGHHLGFDEDGLDQMGLA"
     gene            complement(18674..20329)
                     /locus_tag="VGR22_08210"
     CDS             complement(18674..20329)
                     /locus_tag="VGR22_08210"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEV2128586.1"
                     /translation="MPVFVRILAALASISLLGWGLYTIDDVSDARWLSILGVAWLLLL
                     AATYVQLPAMPEIPRSLIRVALVFATVIAIISVQLIRIQVIQQDEIAFRTAISADGSD
                     ALANPRVVLTPLETARGEIVDRDDEVIASTIQEGDLYYRIWPDPATAYVAGYFSPFLV
                     ASAGLEATYADVLAGQTKNDPVRRLFNNLLHQPQVGSDLRLTLDSDLQRAAHEMLGGQ
                     DGAVVVVDVDTGDVLVLASNPNYDPNQLFTTSPSENPMAQSYWDALVGDPNTPLVSRA
                     TIGLFTPGSTFKTVTAAIALERGYAEPDTVYEDDGDIEIDGRVLVEANRPDESRTEWT
                     LREGLMWSLNVVLAQVGLQIGGDTFWEDAQKFGFGEEIPFALPTSISQLASSEAFLDS
                     RNAVADTGFGQGQIQVTPLHMALITAAYANNGVMMEPRLVDQIIAPSGGVVETLPARE
                     WLTPISAETAIEVRSMMIDTVASGSAQLAQVPDYTMGGKTGTAETSGEAPHSWFIGFI
                     GDTDSEPRYAVAVVLEEGGTGLSGPIGMGRDMLVQAMQQMPSE"
     gene            complement(20356..21675)
                     /locus_tag="VGR22_08215"
     CDS             complement(20356..21675)
                     /locus_tag="VGR22_08215"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV2128587.1"
                     /translation="MTGRFRPTFRATEFGLLLLVAIVAVIGVGMIVLVPQAQLDWSWS
                     EIWVGLVFAAAVLLASLSLGIRGFRGDQTVLPITAMISLIGLLMIQRLHPDLAAISDA
                     YASLAQRHLIYLAAGLAIMWLIVMLLGPVGLVRWLQRYRYLILLAAIALQVSTFFIGT
                     EVYGAKLWIEVGPIQIQPSEIVKLALVAFLASYLDENRDLLAASWRVGPLELPPIPYL
                     IPMGIMWAVSLLTLVVLNDLGSALLFFGIFLVMLYIATGGMVYVAAGLMSFAGAVYLA
                     WLVFDRIALRVQNWLNPWADPLGVGFQPVHSDYALATGGIFGVGLGYGSPTYIPEVQT
                     DYIFSAIGEEMGLLGALAVICLYLVFVVRGFTIAMRARDGYVQLFAAGLSSIIAVQTI
                     IIVGGVTRIIPLTGITLPLVSYGGSALLTNFALAGMLLYASGLPRRL"
     gene            complement(21677..>22465)
                     /gene="mtnP"
                     /locus_tag="VGR22_08220"
     CDS             complement(21677..>22465)
                     /gene="mtnP"
                     /locus_tag="VGR22_08220"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEV2128588.1"
                     /translation="QTPFGEPSAPILQGMLGALDLCFLPRHGKGHAHNPSEVPYRANV
                     YALRALGVTHLISISAIGSLKEHLPPGTMVLPDQIIDRTVQRARTFFEGGVVAHVSMA
                     DPFCASFQGRIAEAAASVEVDFHRGGAYVCIEGPQFSTRAESQLYRSWNADVIGMTAM
                     PEARLARESGLCYAIVATVTDYDVWHESEEDVSVEAVLQILRTNVEHGKQLVTDLTGK
                     PLPECEDGCAAAPVNAFATHPETIPVNRRAVVDLLTSRGRENTA"
BASE COUNT         4077 a         6774 c         7578 g         4036 t
ORIGIN      
        1 gtcgcgtcgc gtgaggagcg agcgagtcgc ggcgtggcgc agcttgtcga tctcctcgtt
       61 gatgtcggcg tctttctcga tgaaagtgtc agtgcgcggg acgtacgcct ccggctggta
      121 gtagtcgtag taggagacga agtactcgat cgcgtttctg gggaagaact ccttgaactc
      181 ggcgtagagc tgggcggcga gagtcttgtt gtgcgcaagc accagcgtcg gacggttcaa
      241 gttctggata acgttcgcca tggtgaacgt tttgccggag ccggtcactc cgaggagtgt
      301 ctggaaccgc tcgccgccct gagcgctctc ggtgagctgc tcgatggcct tgggttggtc
      361 gccggttggc tggagcgagc tggcgagctc gaatgcaggc atgttcagtc ctgtcgccat
      421 tcttggctga tgtcggttgg ggtcgtctgg gtgtccggcg aatcgacttc ggcgtgctca
      481 tcggtcgcat ccgcctcagg cggcggctcc aggccgaggt cgtcgcccaa tgtgtctgaa
      541 gttgaggttg acggtgtcgt gtcggtccga atagtttcct gcttggccgg ctgtgcagtc
      601 ggctcgaagc ggtagagctc ggcttcggac tccccgatgc ccatgatctc gtcgatgtgc
      661 cgaacggccc agcgctgtcc ggaaaacggg agtcgcgagg tggcctcgtc ccgatcgaac
      721 cagccagcat cctgaaaatc atcggcaagc gtgacggcgc tctggtcgtt gatggtaacc
      781 gcgagcaccg gcgccagcac gacgacatct cgttcctcgt catagaactg gttgacgaag
      841 tcagccgagt agaaccggtc gatctcaagg ttggctagcg acttgagtgc ggcgcgcgca
      901 gcttccggcg cagtttggcc gtcagcaatc tgatggtgga acgcctgcca cgtctgggcc
      961 atcggcacgt cattgtggcg ctggagcaac aggaattgga cgcgggcgtt gaggcgccga
     1021 aacacgtaga cgtccacaat atcacttgcg aaatcggtca tgcgcgaagg gtgctcccgg
     1081 ccgtgggctc agcgtccgaa gcggtcacgc gccgaatcga gccatgaccg cgcaccgcgt
     1141 ccccgaccga ctggcgggct actgctgatg atacgcccac gccaccgatt ctggtgcatc
     1201 gagtcggtgt ccggtcgccg gaggaagaac ggcacgaaga tcatcgcggc cgcaccgagg
     1261 gccagcaggc tggcggcaaa ttggtactcg ggagcgagcc agacggcgat aagggccagc
     1321 acgagtgccg tgaccagcgg gattcgcaga gcccagtgtc caacgacccg caccgggccg
     1381 gacttgagct ggcatagccg gtgggcggcc gagtgccttc gcgcttccat cgcgttgcgc
     1441 ttccgtgcga cgtgatcgtg aaaggagatc gggcgattgc gcgattgctc gagaatctca
     1501 ttgagttccc ggtccaaccg gctctgcggc tgttccgggg agtcggtact tgacatcggt
     1561 ctcgggctcc tggaacgtat cggtcgtacc cgggttgcca acgaaccctt tagactcgta
     1621 atggtaccgc agcggggcgt ccccgtatgg tgcaggaacg tcctcgaatt cagcaggcga
     1681 gagagtcgtg ggtaactacc gtttcaacgt gatcgacgaa gagggcacgg cgagttttct
     1741 cgggccgcgc catggatcga agatgatcgc tgccgcctgc ggcgccaacc accgctcgct
     1801 ttcgtcactg ctcacctata tccgcgcgct cgacgatgtc tgggcgacgg aggtggagtc
     1861 cgggctgcgt gcgttcgacg agcggaacgg cgagaccgag gccgagactc cgatgcccga
     1921 cccggagagt ggctggaagg cgccgttccg ggtagtagat tcggagacgc gccgtcaaag
     1981 catgaagatt tcgtcgctgg gactgttcgt ggtgaatctc aaggcccggc gcatcgtgca
     2041 ggtagacaac cggtcgcact cgctggagaa atcggggaag ggccgggtgc gtcgcaatgg
     2101 ccggccgaca cggatgctgt tccgctacga tcttcccgcc gaatgggatc tggtaccgtg
     2161 acgattctca caaccgagcg cgtcaaatgg accctctcga cagcgctcgg ggcaggctcg
     2221 catcagctcg cctttcgctc gaaatgacac ggtgggatgc gagcggaatc gcgtcccgcc
     2281 ccggcggtag ggctaccgct tgccgttgct tttctcgcct tcgcggccgc gccggcggaa
     2341 cccgccgaag aacatccgct gcatctgctc tccgacgccc tcgtcagaga ccgaccgttg
     2401 cgactcggtc atcgcttcgt cggatcggct gacgggcacc tcgaggcgcg cacgggcgtc
     2461 ctgcgcctgt tcgaaccgct tgaagagcgt gtcagccagg ttctcgtcgt tggcgaccag
     2521 ctctcgacgg accgtctgca gctcgccggt gagtctgtcg atccagagca cgatggcgtc
     2581 ccggttggtc atcgcgatgt cccggtgcat ctcggggctg ccgagggcga gccgtgtgct
     2641 gtccttcagc ccggtggacg cgagattgcg catgtcgcgc cacgctggat ccgacgtgac
     2701 ggcgttgacc agggagatcg ccgtgacgaa tggcaggtgg ctgacagctg cgacatagct
     2761 gtcgtgctcg gtggggtcga cgaagtagga atcggcgccg gtggcggcaa cgatcccgag
     2821 cacattgcgg atggcgtcct cgctggcgct caccgacggg cagatgaccc aggtggcgcc
     2881 gcggaacaag tccgcgtcgg ccccgtcgag cccttccgac ttgcctgcca ttgggtgccc
     2941 gccgacaaac gagacggtac tcggcaactc cttcgcccat tcaaggacgt cggccttggt
     3001 cgagccagtg tcggtgacga tcgcgcccgg cttgaggaac tcggcgatgt cgctgaagac
     3061 ctcacgcatc gtctgcactg gggtcgcgac gatcacgacg tcggcgtcgc tcaccgcctt
     3121 gccgagcgtc cattcggtac cgtcgatggc acccatcttc ttcgccttgt tctgctggga
     3181 gatctgttca tcgaaaccgg tgatacggag ggcattgctg ttttcgcgcg accaccgctt
     3241 cagagcgagc ccgatcgagc cgcctatcaa tcccagtccg atgatggtga cgttctgtgc
     3301 catgggatgc tcccttccag cgtgcggcaa tgcgcgaggc ggactacggc tcgtactcgg
     3361 tcacgtcgat cgcgcgaatc catccctcga ggccctcctc ggtcacgaat tgcatccagc
     3421 gctcgccatc gcttgggctc tccgtagggg cgtcctcgtt gaggtactcc agcggcgtgg
     3481 agggcgggag ggcagtgacg atgtccgtcg aggtggatgg cccggaccga aggtttactg
     3541 tcacacctcg caactggtag tcggggttga acccctcgtc agtatcttgg tcttcgctgt
     3601 cttgcgactc ggcttcggct gccgaggtcg gggtgggatt cgacgtttgg gtcgaattcg
     3661 cgtccccgct gttcggctcc gcgacaacct gctcgatgcg ctcatcggtc tctccggggt
     3721 ttggctcctg cgccagctgg gcgacggttg gttgggtctc gtcgccttcc ggaatgaagt
     3781 cgttggtgaa gctttcgagc gcggagtaga tcatggtgaa cgtcaccgca cagagggcga
     3841 tggtgaggag catcgagagg ccgatcatga atccggcgcg gcgagagcgt tggcggagac
     3901 ggtaggagag tcccggatct gacatgcgtt cctctcggca agtgaggggg ggtgcggcgc
     3961 ctgcgagact gagtggcaca ggaaaaggct cacctgcagc gaggtgtgcc gtgtcgacgg
     4021 caacccgcga cgcgttgttt ataccatggt gccctcggtg cccttccgac cgtgccgagc
     4081 ctacgaagca cgtcgaccga gccgcccagg agtggtatag tgaacacgcg taattgatct
     4141 ttcgtgatca aacgtgggcc accgcccacg tttcttggtg ttcatccggt tgaattcaca
     4201 cggtgcgttc ggaactcgac aattacgcgc agcctgcgcg caccgtctca ccgtcggcga
     4261 tgaaccgtct gtacaagtga agaggaggtg cgtggcatga agagtgatct ctacacggcg
     4321 atcgcccaga ttgcggcgga gcgcggaatc ccgcgcgaag cggtcctgtc gtccgtacag
     4381 gaagcgttga catccgtata caagaaaagc gccaatagcg aagaagacgt gcgcgtggag
     4441 ctcgactcgg cgaccggcga gatgcaggtc gtcgtggtga agacggtcgt cgagaacgtc
     4501 gaaaaccccg acctcgagat ggcggtcgag gaggcccagc agtacgtgcc cggcgcgcag
     4561 ccgggcgatg tggtgcgcat ccagcgcacg ccggagaact tcggccgcat cgctgcacag
     4621 accgtcaagc aggtggtcca ctcgcggatt cgcgatttcg agcgcgagaa catctatcgt
     4681 gagtaccagg acaaggaggg cgaggtcctc aacggtgtcg tgcagcgggc ggacagccgc
     4741 gcggtcatca tcgagctcgg caaggccgag gcggtgatgc cggcgcgcga gcaggttccc
     4801 tcggagcgct accgcaccaa tcagcgggtc aaggtgttgc taaccgaggt caacagccac
     4861 tcacgtggtc cgcagctgat tgtcagccgc tccgatccgc gcctgatccg ccggctcttt
     4921 gagcaggaag tgccggagat ccagagcggc gcggttgaga tcatggagat tgcccgcgag
     4981 cccgggctgc ggtccaaggt ggccgtggct gcccggcagg aaaaggtcga tccggtcggt
     5041 tcctgtgtcg gcgtacgcgg tgtgcgtatc cagaacatcg tcaacgagct ctacggcgag
     5101 aagatcgatg tgatcgaatg gtcgccggac atggcgacgt tcatcgcgaa tgcgctgagc
     5161 ccggcgaagc cgacctctgt ctccctcggc gaagagggcg accagaaggt cgcgacggtg
     5221 atcgtcccca ccgaccagat gtcgcttgcc atcggcaagg agggacagaa cgcccgcctg
     5281 tcgttcaagc tgaccggttg gcggatcgac atcaagggcc cggagtcgct gctcgaggca
     5341 ggcgaggact tcttccgcag cgctcgcgag tcggccggcg agatgatgcc gggcttcggt
     5401 tggcagggtc gccagccgcg tgccgttcag gtggatggca ccttcacggt ggccggcaag
     5461 acgtacggac cgctcgacga ggagctggcg cgtcgtcagg ttgacgtcga cgtcaaggac
     5521 ggcgttgtcg aggtctacta cgagcgcgac ctgcgggcgc ggttcgacca ggagaccggt
     5581 gaggagctcc ccgtcgatgg cgatgatgcc ttcgaggccg cctggaaggc ggcccagccg
     5641 gcagtggatt ccgactagga cgaaaggggg gcaggctcgt gaatcagccc caagcacggg
     5701 cacaggcgcg caagcgccag aagggcccgc ggccgcagca tgtgccgcag cgcacctgcg
     5761 tcgcctgccg ggagaagggt gacaagcgcg gtctgatccg catcgtgcgg tctccggagg
     5821 gggagatcgc aatcgaccct accggcaagc ggaacggccg cggcgcctat ctgtgcaaca
     5881 agccgggctg ctggcggaag gcgctcgaca cgccgatctt ggcgggagcc ctgaacgccg
     5941 atattccgga agctactgaa cgtgaacttc aggagtacgc tgcagccctt gaggacgggg
     6001 aagcgtccaa gggcagcgcg gctgctctat aaggagacgg cattatggct gatacacgac
     6061 gacgaccgaa tcgcgtctct cgctccggcg gtggtcgccg gggtcggcgc agcggcagtg
     6121 gatcggcgcg taccgtagcg gtgaagcgcg ctgccccggc cgagcgcgaa ccagtagtcc
     6181 ttcctgccgt gatgtcggtg tccgagcttg ccgacaggct cgagatgagc ggcatcgagg
     6241 tgattcgaga gctgatgaag ctcaatatca tggcgagcct caaccagcag atcgactacg
     6301 atacagctgc caaggtcgca actgagctcg gctgggaaac ccttgaggag gagtccggga
     6361 cgcggtcggc tgtggccgat ttcgaggctc ggcggctcga gagcgagcag gatccggacg
     6421 ccagggcgcg tccgccggtg atcacgatca tggggcacgt cgaccacggc aagaccaagc
     6481 tgctggacac gatccggagc accaaggtcg ccgagggcga ggccggcggc atcacccagc
     6541 acatcggtgc ctaccaagtc gagacgcagg gccggaagtt gaccttcctt gataccccgg
     6601 gtcaccaggc gtttacggcc atgcgtgccc gaggtgccca ggcaacggac attgccgtgc
     6661 tggtggtcgc tgccgacgac ggcgtgatgc ccaccacccg tgaggcgatc gcacacatca
     6721 agagcgcgaa cgtgccgacg gtggtggcgg tcaacaagat cgatctgcct gcggccaatc
     6781 cggaccgcgt caagcagcaa ctctccgaac tggaggtcat gcccgaagag tggggcggcg
     6841 acatcccgtt cgtcgaggtt tccgcgctgg aaggactcgg catcgacgat ctgcttgagg
     6901 tcctcctgct ggtcgccgat gtcaacgagt tgaaggcgaa cccggacaag atcgccacag
     6961 gcgtggtggt cgaggctcgc gtggagtcca gccgtgggcc ggtgaccaca ctgctcgtgc
     7021 agagcggcac gctgaatctg cgtgacgtca tcgttgcggg aagcacgtgg ggccgcatca
     7081 aggcaatgtt cgacgatcgt ggcaagcgca tccggcgagc cggtccgagt gcaccggtcg
     7141 aggtgctggg actcatggag gttccagagg ccggcgacac cttcatggcg ttcgaagatg
     7201 agaaggccgc ccgccagctc gccgagcagc gccaggcgca gcgtcgcgtg gagtctctga
     7261 ccgagaaccg gccgctgaag ctgaccgagc tctacgatca ggttcaggag ggtaccactg
     7321 cagagctccg ggtgatcgtt aaggcggatg tgcaaggttc gctcggcgct atccagggct
     7381 cgctgctcaa gctcaatgag gagacagacg cgggggtgac gctctcgatc cagtacgcga
     7441 gtaccggcgc catctcggag tcggacatca acctcgcctc gacgacgaac agcatcgtca
     7501 tcggtttcaa cgtccggccc gacgctgccg cgaagcgcgc tgcagacgtg gccggagtgg
     7561 atgttcgcta ctacacgatc atttaccagt tgatcgacga ggtgaagcag gcgatgaccg
     7621 gcctgctggc cccggtgttc gaggacgtca ccgacggcta cgctgaggtc cgggagacct
     7681 tcaagctccc gaacaacgat gtcgtggccg gactctacgt tctcgacggc aggatcaacc
     7741 gaaattcccg ggcccgtgtg cttcggagtg gtaccgtggt cttcgagggt ggactctcct
     7801 cgctcaagcg gttcaaggac gatgtccgtg aggtttcggc ggggtacgag tgtggactcg
     7861 ggctcgaggg gttcaacgac atccaggtcg gtgaccagat tgagttcttc caccgcgaag
     7921 aggttgcccg cacctagcgg gaacgcgtcc cttcgctgca tccaccacgt cgtgaagtag
     7981 aggtggaatg aatcgtgaca cgacgaacac gccaggtcgg tgagctgctc aaggaggagc
     8041 tcaccgacat cattcgcaag gatgtcaaag atccgcgcat cggcttcatg agcatcaccg
     8101 aagtcgacgt gacacccgat ctccgccagg ctcgtgtcta cgtgagcgtg ctccacgagg
     8161 aggagcgcga gggaacgatg gatgcgctcc gctccgcggc atcgttcatt cggcaccatt
     8221 tgaagccccg gctgcgcatg cggcagatcc cgttccttga gtttcgaagc gaccgctcca
     8281 tggagtacgc ccagcgcatt accgaaacgc tccgagagat cgagcgtcag gatgctgccc
     8341 gggcgagccg tgaacctgcg accgaggctt ccagtgaaca ggagcattca tgaccggtgc
     8401 gttgtggcga ctcgaccgcc aagctgcgga ggacgcgctg gagctcctcc gcggcgcgtc
     8461 ccgtgtggta gcgccgaccc atcagaacgt cgacgctgat ggtctgtcgt ccgcgatagc
     8521 gctgcgcctc gggctggccc aactcggcgt ggagctcgtt ccggtggtgt cggatgggca
     8581 gttgccgcgg tcgctccggt tcctgcccgg catcgatgag gtgctggtct atggtaagga
     8641 cgacctgccc gactacgacg tactgtgcct ggtcgatgcc tcggacaagc gcaggcttgg
     8701 cgacttctac ggcgacgatc cggagcgcgt gtccggcaac gatgtgccga ttgtcaacat
     8761 cgatcaccat gtcaccaatg accggtacgg caaggccaac atcgtggagc ccagtgcggc
     8821 ggcggctgcc gagatcgtcg ccgacctgct gatggagtgg ggcaccgagc tcaaccgcga
     8881 tattgcccag aacctgcttg cgggcatcta cggcgacacg ctcggcctcc ggaccgactc
     8941 gacaacttcc cgaacgctgc ggacttctgc agatctcgtg gatgccgggg gcgacccaag
     9001 ccagatcgtc gacgcgctct tccgtatcaa gccgcggtcg tccgtctgcc tctgggagaa
     9061 ggcactgcgg aatgtgcagt acacgggcgg cctgatctgg accgagttga cgaaggagac
     9121 gctagatcgg tgtggtgccg acgcctccga ggccgaggga atggtcaact tcctcgtcgg
     9181 caccgagggc acccacgcgg ctgcgctgct gtacgccaat gacaagggtt ggcgcgtcag
     9241 cctccggagt ttgccgagcg gtgttgacgt cgcggagctc gccgccgagt tcggcggcgg
     9301 agggcatccg cgcgctgctg gcgtgcaaat cgaggggggc gaggaagaga agcgcgcctt
     9361 cgtcgaggtg gtcggcagac tcgcccagga gcacgcctga gcgaaatggc ccgcaagcgg
     9421 acgccgccgc accaaagcgg ctttctggtg attgacaagc ccgccaagtg gaccagccac
     9481 gacgtcgtcg ggcgcgtcag gcgtgtcatg ggcgagcgac aggtcgggca taccggcacg
     9541 ctcgacccgg cggcgaccgg cgtgcttcct gtggccgtag gccatgcgac caaactcctg
     9601 ccgttcatcg aggacacaga caagacctat atcgccaccg tccggtttgg cgtggtcacg
     9661 gattctggtg accgcgacgg gcggctcctg gcccagcccg cagcagacca tgtcaccgag
     9721 gcgggggtcc gggcggcgct ggatcgattt cggggtgaga tcggccaggt gccgccgatg
     9781 cactccgcga tcaaggtcgg cggtcgcaag ctctacgatg tcgcccgcag cgggggcgac
     9841 atcgacgtgc cggtccgtcg cgtgtacgtc cacgatatcg agttactgga atgggagcac
     9901 cccgcagcga cgatccgggt ttcctgctcg gccgggacgt acattcgcac gatcgcccgc
     9961 gacctcggtg aggcggtcgg ctccggagcg atgctctccc gtcttactcg caccaaggct
    10021 ggcatgttcg ggctacgcca ggcgctctcg atccacgatc tcgaggcgag ccttgattgc
    10081 tgggggtggc ctatggtctc gttgcatccg gacgcggtct tgccggacgc cgacctgctg
    10141 attctcgacg agggggagga gcggcggtgg ttcaatggcc tgcccatcga acggctggca
    10201 atgacgccgg tcgttcgcgt gtatgattcc cggcgggagt ggacagggat cggtctccgc
    10261 gacgatgacg gtgtccggat ccacccgaag cgggtcgtac gagcgagaag cgagtgagca
    10321 tgacggcgga tccgctggaa ctgttgcgac aacccgttgg ccggttgcgg gtggcaacca
    10381 tcggcacatt cgacggtgtc caccgcggtc accaggcgct tatcgaatac gctgcctcac
    10441 gcgcccgcga ggccaaggtt cccctgacgg taatcacctt cgatccggtg cctgcggccg
    10501 tgctgcggcc ggagcggttt ccgggagcaa tcgctccgct aggcgagaag ctgcgccagc
    10561 tggaggccgc cggcgcggga gagatcatcg tgctgccctt cacacccgag ttgtcgtgtg
    10621 tcaccgccga ggccttcatg gacgtgctcg cgaacgacgc gcgcgtggcc gagctctaca
    10681 ccggcgaggg ctttgcgctc gggcacaagc gccagggcac ggttgacgtg ctgaaccaca
    10741 tcgcagcgaa gcgggggatg cgcttcgagg cgattccccg cattgaggat ctccacggcg
    10801 tcgttgccag cagcgaaatt cgccgagcga tccagcgggg cgatgcaaca ctggcagcgt
    10861 ctctgctggg acgctggttc cgcgctgagg gcgaagtggt ccgcggtgcc caggtcggcc
    10921 ggcagatcgg ctatcccaca gcaaacatgc tgcccgccga ggacatggtg cagctcgcgg
    10981 acggtattta cgcgacgctc tcctggcttc caggcgagga gcacgcgcgg cactccatga
    11041 cgtacatcgg tacccggccg gcactgaaca ccggtcgtcg cttcatcgag acgcacgtgc
    11101 ttgacttcga tggtgatctc tacggccgga tattgagtgt cgatttcgtc aagcgtctgc
    11161 ggccggatgc tgattttcct accgtggacg aattgattgc gcagcttcgg cgcgacgagg
    11221 cggaagctcg ccaggtgttg gctcgaacgt cgtgaaaggt gccgcgcggg atctgacggt
    11281 gggggggtgg ttggaacttc gcgcgtgtcg atggtaaaat tcgttctgtg tcgctgacaa
    11341 cgcggtgacg gggcaacgtc accgcagttc gtcgtgtctt gtggacacgt gacatcctcg
    11401 ggaggcaagt tatttccaga ccagctcaga cccgcgtgaa tgatcgcatt cgggtacgcg
    11461 aagttcgact gattgacgaa gaagggcagc agatcggaat cattccgacg cgcgaagcgc
    11521 tgatgatcgc ccgcgaacga ggactcgacc tcgtcgaggt tggcgccaac gccaatccgc
    11581 cggtatgtcg actgatggat tacggcaagt accggtacga gcagagccgc cgtgaccgag
    11641 agaaccggaa gagtcagcat gtgaccaagg tcaaggaggt ccgtgtggag ccgaagatcg
    11701 gcgagcacga tctcgagacc aagggtcgcc aggcagagcg gttcctcaag tccggtgaca
    11761 aggtgaagct ttcggtgctg ttccgcggtc ggtcgatcac tcaccctgaa ctgggccggg
    11821 acctgttgca gaagcttgcc gatcaactcg gcgaggtcgg tgtcgtcgag caaggtgccc
    11881 gcatggaagg gcgctccatg accatgtaca tggctccgac acgctcaaag ggtgacgaca
    11941 aggataagta ggtagggagt attaccgatg gcgaatcgag gcaagttgaa gacccacaag
    12001 ggggcgaagc ggcgcttcaa gatcacgggc accggcaaga tcatgcaggc caaaggcatg
    12061 aagagtcacc gtcgccggaa gaaggcgcct cgtgtgaagc ggcagttcga caagatgttc
    12121 gagatgcacg agagcaacga gaagcgcgtc cggcggatgc tcccgaatgg gctcaagtag
    12181 gtcccggttc acgggagaga cggaggacac tgccacatgg cacgtatcaa gcgtggtgtg
    12241 accagtcacc gccggcatcg caaggtgttg cagcaggtga agggtcaccg cggaacgaac
    12301 aatcgactgt acaagcgggc ccatgaatcg ctcatgcgct cgctggcata ccagtaccgg
    12361 gaccgtcgca atcgcaagcg cgacatgcgc cggctttggg tgcagcggat caacgccgcc
    12421 agccgagcca acggtctgca gtactcacgg ctgatccacg gcctgtcgct ggccggcatc
    12481 gagatcgacc gcaagatgct cgccgatctc gcggtgcgcg acgaggaagc ctttacggcg
    12541 gttgtcgacg ctgctcgcca agccctgccg gctcaggcgt agcacgacgc gttcgtgtca
    12601 tcggatcgct cttcggagcc gataatcatc acgagcgctg ccaacccggc aatcaggatg
    12661 atgcgctcgc tcgagcgccg gagataccgg caagccgagc gagcgtttct tgttgagggg
    12721 cgtcgcctgg cggaggaagc gcttcgcatg gcgcctgaca ccgtgcgcct cctcctcgtg
    12781 cgcgaggacg tgccgtctgg ctggtggcgg gagttcggtc tcgacgcctc gaaggtccgg
    12841 cgcgtggacc ggaaggtgtt cgaagctgcc agcggggttg agcacgctca gggagtagcg
    12901 gctgtctgcg acctgccagt tcctgctggt attgacgatc tgcgtcgcgc agttgggccg
    12961 gtcctggtcc ttgaccgcct gcgcgacccc ggcaatgtcg gaaccgcgtt gcgctcggct
    13021 gcagcggctg gtgccgagat cgtgcttacc acccctgcta ccacggactc gtggtcgccc
    13081 aaggtcgttc gggcaggaat gggagctcat tttcgccttc gtatcggcgc ttttggggaa
    13141 gatgcggcat cgttcctgcg ggaaaccatc catgatgtag tctgtgccga cgcgtcggcc
    13201 ctcgagccct accatgctcg ggactggcgc tccgcggcag cgctcatcat tggcggcgag
    13261 accgagccgc tctcagcgga gttggagacg ctcgagacac accgggtcag aattccgatg
    13321 cgaggcggca tggaaagtct gaacgcgggg gtcgcggcct cggtcttgct attcgaagcc
    13381 caacggcagg gcgcgtacga acggtgatct ctgccgggtg tcgacagggg gctgtagttt
    13441 cggtactatc cgtgattgtt ggacagacaa tccggatact gggcgggagt gatcgctgat
    13501 cgctcgctcg cagaaaggaa gggcgtaata tggaaaacca agagactgca aaggcactgc
    13561 gcaagcaaga cctcatcaag gcagtggcgg aagaactcgg caggagcgag tccgaggccg
    13621 gcaatgccgt gaatgcggtt ttttcggtga tcgaacgcag cttggcgaat ggcagggacg
    13681 tcaccgtgag cggctttggc tccttcaagg tcgtcgagcg ctcagctcgt gagggtcgta
    13741 accctcagac gggtgaggcc atgacgatcg ccgcgaagaa ggccccggtc ttccgcgccg
    13801 gcacccagct gaagcgctct gtggagagcg cgcagggtgc cacccttggg tagctttcga
    13861 gcgtgaaatc gagcaacagc ggcccgggaa accgcccggg ccgctgtggt tgctccattg
    13921 agaggcatcg aggcgattat tcgcgtggcg gtggatacaa cgtgagcctg taacggcggt
    13981 ttgtgggctg tcgtcatgaa taaagacggg gtagcgatgt cggcccaggg ctatcggtcg
    14041 gcgcaacgcc gcgttgaagg cgctaggttg ccggcaccag cggggtaggg gagtcgaatc
    14101 tacgcctctt gggctgtttg agcctgctcg gaaatctcgg agacgcgctc ggcgatacgg
    14161 gacgggagtt gctgttcgat gctgcgaagg gcgacaccga tcgcgttttc aacggccagc
    14221 gcattggacc ggccatgcgc gatgatggtg ttgccatcca cgccgagcag gggggcgccg
    14281 ccgatctccg cgtagtcgag ccgcctggcg acctcccgaa atgccggttt gagcatcgcc
    14341 gctccaagct tgcggaaggg gctggccctt agctcgctct tgagggcgtc gaggagaaag
    14401 gccgcagtgc cctcagcggt cttgagggcg atgttgccgg tgaacccgtc ggctaccacg
    14461 atgtccgtcg tgccgagcat gatgtccttg ccttcaacgt tcccgtggaa gtcgatgccg
    14521 ggtgtctgct gcagcaactc gtgaactctc acggtcaact ggttaccctt gctgccttcc
    14581 tcgccattgc tcaggaggcc aattgttggc tcgggcacac tgagcacgta gcgggcgtag
    14641 gtttgtccca tgattgcgaa ctggaccatg tgcgccggtt tggggtcagt cacggcgccg
    14701 aggtcgagca ccagtgcccg ccccgccttg gacggcatgt gcgaggcgat ggcggggcga
    14761 tcaatgccct tgacgcgccc caggttcatc agcgcgccag ccatgacagc gccgctgttg
    14821 ccggccgaaa ccatcgcacc ggcttcccca tcctgcaccg ccctgattga gcgcatcagg
    14881 ctgctctccg gtttgcgccg gaccgcctgc gtcggatgct cgtccatgcc gatcgtgtcg
    14941 accgtcggga ccggttcgac atgcagcccg gcgagatcgt gttggcccag cgcgatatcg
    15001 agcgcggact tgtcgccgta gagccggatg ccgacgctga agcgccttgc agcggcgacg
    15061 gctccgccga ccacgaccgt cggaccatgg tcaccgccca tggcgtcgac agcgatgatg
    15121 gccggttcgg taggcatggc gcggcgctcc ccctcgtgct gtggcggcta gatgtcgagg
    15181 ttctggacgt cctgcgcgtg tgcctggatg tacttgcggc gcggcggaac cgcggcgccc
    15241 atcagcatat cgaacgtctc gtcggccgcc tggccgtcct cgatcgtcac ctgcttcagg
    15301 gtgcgcttcg ttggatccat ggtggtctcc cagagctgct ccgggttcat ctcaccgaga
    15361 cccttgtagc gctgcacctc gcggttctca ccgcgatcct tgagcacctg ctcgagctca
    15421 cggtcggaga agacccactc ctcgctgttg ccgcgcccga ccttgtagag cggcggctgg
    15481 gcgatgtaga caaagccgtc gcggatcagt ggctccaggt gccggtagaa gaacgtcagc
    15541 agcaacgtgc ggatgtgcgc accgtcgaca tcggcgtccg tcatgatgat gatgcggtgg
    15601 taccgcagct tcgagatgtc gaactgctca cccaccgagg taccgagcgc tgtgatgatg
    15661 gtccggatct cgttgttctg cagcatcttg tccagccggg ccttctcgac attgaggatc
    15721 ttgccccgca gcggcaggat tgcctggaaa cgtcgctcgc ggccctgctt ggccgagccg
    15781 ccggccgagt ctccctcgac gatgtagagc tcggacctcg atgggtcacg ctccgagcag
    15841 tcggcaagct tgccggggag actgaagttg tccagcccgc ccttgcgctg cacgagctcc
    15901 cgcgcctttc gagccgcctc tcgtgcgcga gcagcgttga ggcacttctc gatcacacgg
    15961 cgggagacct gcgggttttc ctccaggaac gcgccgaacg actcgttgac gacgctctgg
    16021 accgcccccg ccacctcggc gttgcccagc ttgcccttgg tctggccctc gaactgcggg
    16081 tcctcgatct tcaccgagac gattgcggtc aaaccctcgc gaacatcgtc accggagagc
    16141 gagtcgtcgt tgttcttgag gaagccgttg cgcctcgcgt agtcgttgat ggtccgcgtc
    16201 aatgcggtct tgaagccggt gaagtgggtg ccgccctcga tggtgtgaat gttattggcg
    16261 taggtgtagg tcgtctcgcc gaacgaattg ttgtactgca acgcgacctc gacatcgacg
    16321 acaccctgag gcgtctcgat ctcgcgatgt acggaaaacg gtcggtcgtg cagcggctgc
    16381 ttcgaccggt tcaggtgccg aacgaaggag ttgatgccgc cctcgaagta gaaggacatc
    16441 tccttgtcgg tgcgctcatc cttcagcgtc agcttgagcc cgcgggtgag gtaggctgtc
    16501 tcgcggaacc actgagcgat cgcgtcgaac tggtagttga gcccgctcat gaagatctgg
    16561 gtgtccgcga ggaagtgggt ggtggtgccg tgcgagctct ttgtgtcttt gccgatgacc
    16621 tgtaccttgg tagtcggctt gccgcggctg tactcctgct tgtaaacgtt cgggtcgccc
    16681 ttctttcgga cctcgacggt cgtccactcg gagagcgcgt tgaccacaga ggcgcccacg
    16741 ccgtgcaggc cgccggagac cttgtacccg ccgccgccga acttcccgcc cgcgtgcagg
    16801 tgggtcatga tcagctccag cgcggacttg ccggacttgg tatgcgtatc caccgggatg
    16861 ccgcggccgt tgtcgaccac ggtgatcacg ccatccgcgc cgatggtgca gacgatgtgg
    16921 tcagcgtgcc ccgccagagc ctcgtcgacc gagttgtcca cgatctcgcg gatcagatgg
    16981 tgcaggccgc gctggtccgt atcgccgatg tacatgccgg gacgccggcg cacaccttcc
    17041 aggccttcca ggacctggat ctggcttgcg tcatatgcgg tttcgttgag tgcttccttg
    17101 acttgggttg ccattccggc atctccttct tggcccgtcg gggcctgacc gctagagcga
    17161 cgtgcgatgg tattactttg agccgggagc ggtctcgaac ttccgaatga tgcgatcgcg
    17221 atcgtaatag aacatcatgg tagcgatcgc gatgcccgtt ccgagaaaac tgttgaccag
    17281 aattgcgatc ggcaggcccg gcaccgagga ggccatcgtg tcccacaggc ggagggcgcc
    17341 caccgcgatc agcatgacgg cgatcgagaa ccgaacagtc ggtccgaaaa agtgatgtgc
    17401 gaggcgtgcc gatcgtcgaa gggcacgcag tggcccgata tcattgacga caatcgattc
    17461 gagcacgaac cgggtgtaaa tcgcgatgag acttcccagc aacaccagga aaagcgagat
    17521 cagcgccgcc aggttgacct gcattagcag cagtaccccg gcgccaatca tgaccggggc
    17581 aatcaccacc atcgcgccga cgatcatgag cccgatcagg cccagaaagc gcagccagga
    17641 acgcgctacg tagcgcgcca cctcgatcgg ccgcattgtc cgcccagcga ccaacagggc
    17701 cagcggcacc cgccacccga cagtcagcag ggtcgagacg aggaagaaga gcaaaccgag
    17761 acccaagatc gaccagattg atccagggtc tcgcacagct cccgttgccg ccagccatgt
    17821 gtcggcgagg tcgcctcggt cgacgccatc ggcaagtccg ggcgtgaaga ccgccatcat
    17881 cacgttgaac aggttgtcgt gcgacaaccc ggcgaacacg gaaggcagca ggctgcccat
    17941 gacatcgttg agccaaaagg tttcgcccac ctcacggaac tgctccgcgg ccagatcgcc
    18001 gttctcccca ccaatcgctt ccatctcgtc agcaagcggt gtaatgagcc gcgtgatcgg
    18061 aatttgcatg ccgacgagga gcaggacatc gagcaggatc gggagcacca gcaaccatgg
    18121 ccgtgagagc ggtacagaca ggccggatgc aacggtctcg gtcacgccgg aaatgttccg
    18181 cgaagcctcg cggatcttct gtccagcgct gggagagagt cgttgaatca cgctaatccc
    18241 atctgatcca ggccgtcctc gtcgaacccg agatggtgcc cgagttcgtg caggacggta
    18301 atccgaattt cctcatccaa ctccggtccg atgccgaacg cacgggtgag cgggccccgg
    18361 aagacggtaa tctggtccgg aacgaccatc gagtacccgc cgtagcgctg gctgcgggga
    18421 atgccgtggt acaggccgaa gagctcttcg ctctcgccgg cgggactggc agcgggtggc
    18481 tcgtcttcga cgacgatcgc aacattgtcg aggtactgcc ggtattgctc cggcaggtat
    18541 ctcagcgcgg cgtcgacacg gcgcgaaaac tggagccgac gagccttggc tcgcaaggct
    18601 gctaccctcc gctgcccttc tattttacca gttttcatgg ggattctcca tcgtttccgt
    18661 agtgcgcgtg gtcctattcc gaaggcattt gctgcatcgc ctgcaccagc atgtcgcggc
    18721 ccatcccgat tggtccggag agccccgtgc ccccctcttc gagtaccacg gcgaccgcgt
    18781 agcggggctc gctatctgta tcgccgatga agccgataaa ccatgagtga ggagcctcgc
    18841 cgctcgtctc ggccgttccg gtcttgccac ccatggtgta gtcggggacc tgggccaact
    18901 gcgccgagcc ggacgcgact gtatcgatca tcatgctccg tacctcgatg gcggtctcgg
    18961 ccgaaatcgg ggtgagccac tctctcgccg gcagagtttc gaccacgccg ccgctcggcg
    19021 cgatgatctg atctaccagc cggggctcca tcatcacgcc attgttggca taggcggcgg
    19081 tgatcagcgc catgtgcagc ggtgtcacct gaatctggcc ctgaccgaag ccggtatcgg
    19141 cgacggcgtt gcgcgagtcg agaaatgcct cactggatgc aagctggctg atggaggtcg
    19201 ggagggcgaa cgggatctcc tcgccgaagc cgaacttctg ggcatcttcc cagaaggtgt
    19261 cgccgccgat ctggaggccg acctgtgcca gcaccacgtt gagtgaccac atcagcccct
    19321 cgcggagggt ccattccgtc ctcgattcgt ccgggcggtt tgcctcgacc agcacccggc
    19381 cgtcaatctc aatgtcgccg tcgtcttcgt agacggtgtc cggctctgca tagccgcgct
    19441 caagggcgat ggcggctgtg acggtcttga acgttgaacc tggcgtgaac aggccgatgg
    19501 tggcgcggct gacgagcggt gtgtttggat cgccgacaag cgcatcccag taggactgtg
    19561 ccatcggatt ctccgacggt gaggtcgtga agagctggtt tgggtcgtag ttggggttgc
    19621 tggccagcac cagcacgtcg ccggtatcga cgtcgacgac gaccaccgcg ccgtcctgcc
    19681 caccgagcat ctcgtgggcg gcccgctgga gatcgctgtc gagtgtcagg cgcaggtctg
    19741 agccaacctg tggctggtga agcaggttgt tgaagagccg gcggaccggg tcgttcttcg
    19801 tctggccggc gagtacgtcg gcgtaggtcg cctcgagccc ggcggacgcc accaggaagg
    19861 gggagaagta gccagccacg taggcggtgg ccgggtccgg ccagatgcgg tagtagaggt
    19921 cgccctcctg gatggtgctg gcaatcacct cgtcatcgcg gtcgacgatc tcgccgcggg
    19981 cggtctcgag cggggtcagc acgacgcgcg ggttggcgag ggcatcggag ccgtctgcgg
    20041 agattgccgt gcgaaacgcg atctcgtcct gctggataac ctggatgcgg atcagctgga
    20101 cggagatgat ggcgatcacg gtggcgaaga cgagcgccac tcggatcagt gaccgcggga
    20161 tctccggcat cgccggcagt tgcacgtagg tcgcggcgag cagcaggagc cacgccacgc
    20221 cgaggatcga gagccatcgg gcgtcggaga catcgtcgat cgtgtagagg ccccagccga
    20281 gcaggctgat gcttgcaagg gcggcgagaa tgcgaacgaa aacaggcatg gacatacctc
    20341 tgggcggtgc tggtgtcaga gtcgcctggg caggccggaa gcgtagagca gcattccagc
    20401 gagcgcgaag ttggtcagta gggcggagcc gccataggag acgagcggca gggtgatgcc
    20461 ggtgagcgga atgatgcgcg tcaccccgcc gacgatgatg atcgtctgca cggcgatgat
    20521 cgaactgagg cccgcggcga acagctgaac gtacccgtca cgcgcccgca tggcgatggt
    20581 gaaccctcgc acgacgaaga cgagatagag gcagatgacc gccagtgcgc cgagcaggcc
    20641 catttcctcg ccgatcgcgg agaaaatgta atccgtttgg acttcgggga tgtaggtcgg
    20701 tgagccatag ccaagtccca cgccgaagat gccgccggtg gcgagggcgt aatcggagtg
    20761 gaccggctgg aagccgacgc cgagcggatc tgcccacggg ttgagccaat tctggacgcg
    20821 gagtgcgatg cggtcgaaga cgagccatgc caggtagacc gctcccgcga atgacatgag
    20881 tcccgcagcg acgtagacca tgccaccggt ggcgatgtag agcatgacga ggaagatgcc
    20941 gaagaagagc agcgccgagc cgaggtcgtt cagcaccacg agggtgagca gcgacacagc
    21001 ccacatgatg cccatcggga tcaggtaggg gatcggcggg agctcgagcg gcccgacgcg
    21061 ccacgaggcg gcgagcagat ccctgttttc gtccaagtag ctcgccagga aggccaccag
    21121 ggccagcttg acgatctccg acggctgtat ctggatcggc ccgacctcga tccagagctt
    21181 ggcgccatac acctcggtgc cgatgaagaa tgtcgatacc tgcagcgcga tcgcggcgag
    21241 gaggatgagg tagcggtagc gttggagcca gcggacgagg ccgaccgggc cgagcagcat
    21301 cacgatcagc cacatgatgg cgagaccggc ggccaggtat atcagatggc gttgagcaag
    21361 ggacgcatag gcgtcgctga tagctgcgag atccgggtgg agccgctgga tcatgagcag
    21421 gccgatcaac gagatcatcg ccgtaatggg cagcacggtc tggtcgccgc ggaagccgcg
    21481 gatgccaagc gagagactcg ccagcagtac cgcggcggca aagaccagcc cgacccagat
    21541 ctccgaccat gaccagtcaa gttgagcctg cgggacaagc acgatcatgc cgacgccgat
    21601 caccgcgacg atcgcgacga gcaggagcag gccgaactcg gttgctcgaa aggtgggacg
    21661 aaaccggccg gtcatctcag gcagtatttt ccctgccgcg agaggtcagc aggtcgacca
    21721 ccgctcgtcg gttgacgggg atcgtctcgg ggtgggtggc gaacgcattc acgggtgcag
    21781 ctgcgcatcc atcttcgcac tctggaagcg gtttgccggt gaggtcagtc accagctgct
    21841 tgccgtgctc tacattggtt ctgagtatct gcagcaccgc ttcgaccgag acatcctcct
    21901 ccgactcgtg ccagacgtcg tagtccgtca cggtcgcgac gatggcgtag cagagtccgg
    21961 actcgcgggc gagtcgtgcc tcgggcatcg cggtcatgcc gatgacgtcg gcgttccagc
    22021 tacggtagag ctgtgactcg gcgcgagtcg agaactgcgg tccctcaatg caaacatagg
    22081 cacccccacg atgaaagtcg acttcgacgc tggctgctgc ctccgcgatg cgaccctgga
    22141 acgacgcgca gaagggatcc gccatggaga cgtgcgcgac aacgccgccc tcgaaaaagg
    22201 tacgagcgcg ctgcacggtg cggtcgatga tctggtcggg cagcaccatc gtgcccgggg
    22261 gcaggtgctc cttgaggctg ccgatcgcgc taatgctaat cagatgcgtt acacccagcg
    22321 cgcgcagggc atagacgttg gcgcggtacg gcacctcact cgggttgtgg gcgtggccct
    22381 tgccgtggcg tggcaagaag cagaggtcga gtgcaccgag catgccctgc aggatcggcg
    22441 cgctgggctc cccgaatggc gtctg
//
LOCUS       DASYWL010000229         2073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_611473, whole genome shotgun sequence.
ACCESSION   DASYWL010000229 DASYWL010000000
VERSION     DASYWL010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611473"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VGR22_08225"
     CDS             complement(<1..206)
                     /locus_tag="VGR22_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128589.1"
                     /translation="MPNREYTLARQGEFDARSLDAADPLRGFRERFYREPGTVYLDGN
                     SLGLLSRDAEAAVLAALESWKTHGV"
     gene            complement(240..1745)
                     /locus_tag="VGR22_08230"
     CDS             complement(240..1745)
                     /locus_tag="VGR22_08230"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEV2128590.1"
                     /translation="MRIVTGGIAQETNTFQRAPTTLADFCRPGFGIVARGTEILELED
                     TGTVYGGVIAEAKASNVELIPTLYAEVMPGGRVNRDAFDVLSEEMLSGIRAAAPVDGV
                     LLVLHGAMALEDEDDAEGLLIGKVRELVGPEVPIIASLDLHTNLSDAMVEGADALVGY
                     KQYPHTDMRETGRQALQVLAARVGNVVQPVMAHTRLPLIAPNQTMVTTWQSPLKTAID
                     RARHMEQQPDVLAATVLGGFAFADVPFAGVATIVVTNGDADLARSYADELAAICWNQR
                     DDFTVRPVPVSDATSEAMAAPDGSVYVLADIADSGASGTASDGTAVLKGLLDAGARSA
                     AVAQIKDPEAVAACIEAGVGSRVALRIGGKQDDLHGPPLEVRGMVRLIHEGSFVLAGP
                     MEAGTVSSRGRTVVLEIGGRGGIEVQLTETRGHPCDLNHFRAFGIEPTERRILVLKSA
                     VHFRAAFEPIATKVIEVDAPGISSPRLTSFDYQRLQRPIYPLDSDVSWAPA"
     gene            complement(1848..>2073)
                     /locus_tag="VGR22_08235"
     CDS             complement(1848..>2073)
                     /locus_tag="VGR22_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2128591.1"
                     /translation="SGALIAEGALSFLGLGVQPPMPSLGSLLRDGKTYMELAPWTMLF
                     PAVTLAVAILAINLLGDELQAFTDPKLRSK"
BASE COUNT          345 a          690 c          679 g          359 t
ORIGIN      
        1 acgccgtgcg tcttccagga ttcgagcgcg gcgagcacgg cggcctcggc gtcgcgggag
       61 agcagcccga gcgagttgcc gtcgaggtag acggtgccgg gctcgcggta gaagcgctcg
      121 cggaaccctc tcagcgggtc ggcggcgtcg aggcttcggg catcgaattc gccctgacga
      181 gcgagggtgt attcacggtt tggcatcggc atccctggtg agcgggcgac cggaggctgt
      241 cacgccggtg cccagctcac atcgctgtcc aatgggtaaa tcggacgttg gaggcgctgg
      301 tagtcgaagc tggtcagcct ggggctgctg atgcctgggg cgtccacctc aatgaccttc
      361 gtcgcaatgg gctcgaacgc tgcccggaaa tggaccgcgc tcttgagcac gaggatgcgc
      421 cgctcggtgg gctcgatgcc gaaggcgcgg aagtggttga ggtcgcaggg gtgtccgcgc
      481 gtctcggtga gctggacttc gatcccgccg cggccaccga tctccagcac gaccgtccgt
      541 ccccggctgg agacggtgcc cgcttccatc ggtcccgcca ggacgaacga gccctcgtgg
      601 atcagtcgga ccatcccccg cacctcgagt ggcggcccgt gcagatcgtc ctgcttgcca
      661 ccgattcgca gcgcaacccg acttccaaca ccggcttcga tgcacgcggc gaccgcttcc
      721 gggtccttga tctgcgcgac ggccgccgat cgcgcgccag cgtcgagcaa ccccttgagc
      781 accgccgtgc cgtcgctcgc cgtgccgctc gcacccgagt cggcgatgtc ggccagcacg
      841 tagacgctgc catcgggcgc cgccattgcc tcgctggtgg catccgacac cgggaccggc
      901 cggaccgtga aatcgtcgcg ctggttccag cagatggcgg ccaactcatc agcatacgac
      961 cgagcgagat cggcatcacc attcgtcacc acgatcgtcg ccacgccggc aaagggaacg
     1021 tcagcaaagg caaaaccccc gaggacggtc gcggcaagca cgtctggctg ctgctccatg
     1081 tgccgcgcgc ggtcgatcgc ggtcttgagt ggtgactgcc aggtggtgac catcgtctgg
     1141 ttcggggcaa tgagcgggag gcgggtgtgc gccatgactg gctgcaccac gttgccaacc
     1201 ctggcggcga gcacctgtag cgcttgccgg ccggtttccc gcatgtcggt gtgagggtat
     1261 tgcttgtacc caacgagggc gtctgcacct tcgaccatgg catcggagag attggtatgc
     1321 aggtccagcg atgcaatgat cggcacctcc gggccaacca gctcccggac cttgccgatc
     1381 agcagccctt cggcatcatc ctcgtcctcc agggccatcg ccccgtggag cacgagcagg
     1441 acaccgtcaa ccggcgcggc cgcgcggatg ccgctcagca tctcctcaga gaggacgtcg
     1501 aaggcgtcac ggttgaccct gccgcccggc ataacctcgg cgtagagcgt cggaatcaac
     1561 tcgacgttgc tcgccttggc ttccgctatc acaccgccgt agacggtacc ggtgtcctca
     1621 agctcgagaa tctcggtacc ccgtgccacg atgccaaacc caggtcggca gaagtcggcc
     1681 agcgtcgttg gtgcccgctg aaacgtgttc gtttcctgtg cgatgcctcc agtgacgatc
     1741 cgcatgatgg agcctccatt tcgccggttc accggcaacg tcatgggcgc gtccccccat
     1801 aacaggggcc gcgtggatgt gctgcgcttc tgctcagaca tctcatccta cttgctgcgc
     1861 agcttgggat cggtaaatgc ctggagctca tcccccagga ggttgatcgc caggatcgcc
     1921 acggccaacg tgaccgctgg gaagagcatg gtccacgggg cgagctccat gtaggtcttt
     1981 ccatcacgca ggagcgaacc gagcgatggc atcggcggct ggactccgag gccgaggaaa
     2041 ctcagtgcgc cttccgcgat gagggcgccg gag
//
LOCUS       DASYWL010000230        31353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_105120, whole genome shotgun sequence.
ACCESSION   DASYWL010000230 DASYWL010000000
VERSION     DASYWL010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 31353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31353
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105120"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..672)
                     /gene="pyrE"
                     /locus_tag="VGR22_08240"
     CDS             complement(94..672)
                     /gene="pyrE"
                     /locus_tag="VGR22_08240"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEV2128592.1"
                     /translation="MTQLRDIGALKTGHFLLASGRHSDIYLEKFDLLRNPRATEDVCA
                     GFVEAFRDTDIDVVVGPTTGGVLLAYETARQMGLPAAYAERAKEGSDKREIRRGTTFP
                     EGARVLVVDDILTTGGSVRETLAALKAHPVEVVGIGVLVDRSGGEVRFGDVPLRALTQ
                     MKVDAWEPVRCPLCTNGVPLEKPGTTVVPNNE"
     gene            complement(681..1694)
                     /locus_tag="VGR22_08245"
     CDS             complement(681..1694)
                     /locus_tag="VGR22_08245"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEV2128593.1"
                     /translation="MGITTPRDVTVDLAPGNPRELVLRNPVIPASGCFGYGQEFAGAI
                     DVQRLGAFVSKGITPKPRSGNPMLRIAETPSGMLNAIGLQNPGISKFIRTYPPIWESW
                     EVPAIVNISAESVEDFVDMARRLDDISGVAAIEVNISCPNINAGGFCFGWDAHMSAEV
                     TRAVRAATTMPMIVKLSPGALDVVEIAQAVEEAGADALSLINTFVGMAIDTERAEPVI
                     ANVTGGLSGPAIRPLAVWMVWQVAGAVSIPVIGMGGIVSVKDAIEFFMAGASAIQVGT
                     AAFAQPKVLMELVDGIPAWLVAHDHSRLSDIVGMANPRYLARRQQTAAAGRQLITESE
                     FSQ"
     gene            complement(1685..2491)
                     /locus_tag="VGR22_08250"
     CDS             complement(1685..2491)
                     /locus_tag="VGR22_08250"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEV2128594.1"
                     /translation="MATGQATKLEFYGDIVHVESVMGDSVLTTFEAPEWAVRSSKPGQ
                     FVQILSREPRSTDPLLRRPYSVYRADPVAGTLTVLARPYGRGSAWLCEQVVGTPLDVM
                     GPLGRPFEVRPKTHHLLMVAGGVGAAPLLMLAEEAVRRGKQVTYLMGGQNEEALLPAS
                     ELPDEVEYVVATIDGSRGHSGFVTDLVAEYLGWADQVFSCGPEPMFRSLREVVHHNRL
                     SDRPDVQMAVERPMACGIGACLGCVVETRHGMKTACVDGPAFDMDELLWG"
     gene            complement(2609..5836)
                     /gene="carB"
                     /locus_tag="VGR22_08255"
     CDS             complement(2609..5836)
                     /gene="carB"
                     /locus_tag="VGR22_08255"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEV2128595.1"
                     /translation="MKTPESVLVIGSGPIVIGQAAEFDYAGTQACRALREEGVRTILV
                     NSNPATIMTDEDVADAVYIEPLTPEVIRRIIQRERPEGLLPTIGGQTGLNLAVKVAEL
                     GILDEYGVRMLGTPVESIQMAEDRSQFKALLERIGEPAIPGQIVHSVAEAKAFAREVP
                     LPMVIRPAYTLGGTGGGVAHTEQELEQYVRSGINASPIGQVLLERSLLGWKEIEYEVM
                     RDGADTCITICNMENIDPMGVHTGDSIVVAPSQTLSDKEYQLLRTSSLKIIRELKIEG
                     GCNVQLALDPNSMKYGVIEVNPRVSRSSALASKATGYPIARLSAKIAIGKRLDEMLNV
                     VTGKTTAAFEPALDYVVAKIPRWPFDKFPRADRTISTQMKATGEVMAIDRSFEGAIQK
                     AVRSLETTGKDLGWEDPSWEDNPEKIHELIQRPNDLRLWAIMAALRRGVRPEQIHDLS
                     KIDMWFLWKLDRLVGLERCLSSDELAPELLWEAKQAGFGDIQIANLAGISAAEVRQKR
                     RELGMVPVYKMVDTCAGEFEAVTPYFYSTYEEEDEAAPLKGDRAMVVGSGPIRIGQGI
                     EFDYCSVQAALALREAGVQSIMVNSNPETVSTDFDASDRLYFEPLDRESVEEILYHEA
                     KGKLEDYPPLVLQFGGQTAINLAEPLSEAGSTIWGSSVDTIDLAEDRERFEAFLRSLG
                     IPQPPGATVISLHDAERVAAQIGYPVLVRPSYVLGGRAMEVVYGADQLARYIRNQASL
                     TPEHPVLVDKYLLGKEVEVDAICDGDEILIPGIMEHIERAGVHSGDSFAVYPGVNLYP
                     AEVETIVDYTTRIAKDLNARGLINIQYVIHAGRIFVLEVNPRSSRTVPFLSKATGVPM
                     VKLATNIMLGKTLCEQGYAGGLWPRQPLTAVKAPVFSMAKLRGVEIHLGPEMKSTGEV
                     MGIDRSFDAAFFKALIAAGLSLPAHGSILVTIADEDKSESLDIISALTRMGFRLYATE
                     GTAAMIERAGMPVQMVTKRIGHGSPDMLDVIREGIVDGVINTPGPADKEILDGLEIRR
                     AAVERGIPCITSIDTARTVVEAMEHSGESFTVEPVGSYRRPAFGY"
     gene            complement(5929..7068)
                     /gene="carA"
                     /locus_tag="VGR22_08260"
     CDS             complement(5929..7068)
                     /gene="carA"
                     /locus_tag="VGR22_08260"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEV2128596.1"
                     /translation="MPDRYRPPQDAALALADGRVFRGRGFGAETDASGEAVFTTTMTG
                     YQEVCTDPSFRGQIVCMTYPLIGNYGVNDEDNESRRPWIAGLVVREASEQPSNWRSKG
                     TIDDYLKRADIPGISDIDTRALTRHIRSVGDTRAVLVHNAADLTDEELVERARAAKLP
                     GEQDVVGEVQGHEREAYGAGDGPHVVVIDCGVKRNIVRSLVARGARVTVVPYGTPYAE
                     IEALSPDGVVLSPGPGDPANLDTGLDVVQHLLEDQRPYFGICLGHQLLARAIGANTTK
                     LKFGHRGGNHPVRDEETGRVSITSQNHSYVVDGRTFPEGAGWLVGLRNLNDGSVEGIR
                     HETLPVLSVQFHPEASPGPEDNGYLFDRFLTMIEERKAAEQKQLA"
     gene            complement(7061..8392)
                     /locus_tag="VGR22_08265"
     CDS             complement(7061..8392)
                     /locus_tag="VGR22_08265"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEV2128597.1"
                     /translation="MTRRTLLRGGRVIDPANGWDGVADVVIEDGTVAEVADHTKPRER
                     DEVIDAGGKLVTPAWIDMHVHLRDPGFTGKETLATGGQAAVAGGFGVGGCMPNTSPAL
                     DSPEIIRDVIERAERDGACRVLPIASITKGRTGAEPVEFRALVEAGAIGFSDDGDTTA
                     NSAIMRQALEVSVELGVPVIVHCEDKALATGAMHEGEVSRRLGIEGIPAAAEEIIIAR
                     DVMLAELTGGWLHVCHVSTKRGIELITEAKRRGVRITAEVMPHHIVMSDEWVAGTREL
                     HNTTALAGEPLEPTHGYTKVNPPLRPVADTVGLLEAVQRGEFDIFATDHAPHADPEKL
                     DVPYERAAFGMSGLEFAFPATWELVRAGHVDAIDLVRLWTAAPAEIFGLDRGTLTAGV
                     PADVTLWDPDQAWVVSRDTIWSKSANTPLIGMTLRGRAVRTWINGEEAYRA"
     gene            8647..9324
                     /gene="nth"
                     /locus_tag="VGR22_08270"
     CDS             8647..9324
                     /gene="nth"
                     /locus_tag="VGR22_08270"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEV2128598.1"
                     /translation="MAFSAIDHLDEVERILDETYGPRKLRPSGDPVGTLVSTILSQNT
                     SDINTECAYASLRARFPTWDAVVKANTDEVIDAIRSGGLANRKAPRIQEALDEIRDRY
                     GEFSLDQLATIPLEEAKDTLLSIDGVGPKTAACVLLFALGRPALPVDTHVYRVSKRLG
                     LIDDATTPVRAHATLEALMEGDADRTYRFHVEMIAHGRTICTARNPKCHICPLRSYCE
                     YAATHAT"
     gene            9546..9998
                     /locus_tag="VGR22_08275"
                     /pseudo
     CDS             9546..9998
                     /locus_tag="VGR22_08275"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
     gene            10020..10802
                     /locus_tag="VGR22_08280"
     CDS             10020..10802
                     /locus_tag="VGR22_08280"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2128599.1"
                     /translation="MKLTTIGGSAAGTGTLQGCSCYLVQSDTTSVVLDLGSGTLQQLR
                     AHVDYRTIDGIVISHLHADHIADLVALRFTLSYNPVPAPAPVPLWLPPDGISTLRRIA
                     QAFESPDSGLEWFTDVLDVQEFDPNRSIEVGDLVCTFTPTVHFVPCWAIRVHPENGSG
                     DLFYAADTGPAADLAGFGSGAKVVIAEATDVGEPDLDFESRGHLTAVEAAQLASDIGA
                     ETLVLTHMFEEHDPEGLMAAAEHVFDGTLLRAEPGLEVRWRF"
     gene            10790..11680
                     /locus_tag="VGR22_08285"
     CDS             10790..11680
                     /locus_tag="VGR22_08285"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEV2128600.1"
                     /translation="MEILTSGFRTLDGVRIGNWTDRDAQTGCTVILFDAPALTAAEIR
                     GAAPGTREVDLLRPGMTVQRPDAILLTGGSAFGLAAADGVMRWLQERGRGFPTPAGPV
                     PIVPAAVIFDLAIGEPVAPDATSGYIATDNAVPLDQLAVGRVGAGTGTTHGRIGGSIA
                     IRAGGFAVAQVALPEGTVMALVVLNAYGSLAGADSDPRVAKLDTEAHTPPFGQSTTLL
                     ACLTDIPLDHASLQRMTVAMHDGLARAVAPVHTVADGDIAFATTLLEDPVGPVDRGLR
                     VSIAAELAVEASIGTLAEGT"
     gene            11749..12615
                     /locus_tag="VGR22_08290"
     CDS             11749..12615
                     /locus_tag="VGR22_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128601.1"
                     /translation="MDTNATGSSRLPFRRPTIVVGTESTLLGHPIIAPNQATQAGFYA
                     LDLDLRRAWDTRIPGTLADHAPAACLRSAWIPRQYTGPWREARAVHLREFLTDLVESY
                     GLRTVIVPSADGTRQRGTSLGQLAREVMALGGLRVAMRVGPELLLAQPGSHLDHVANL
                     RRMAEEWDLDLALDLTAPGIEGWEAEATLMRLFPRLTMVRIRPLHSPDGARSQEPNAR
                     IGLRSIAMLADQAYNGVISIAPSVSPLTRWIPAGSRVLVSTALTREEVLAAWDRVDQY
                     DITTWQKRQQFG"
     gene            complement(12833..13246)
                     /gene="rpsI"
                     /locus_tag="VGR22_08295"
     CDS             complement(12833..13246)
                     /gene="rpsI"
                     /locus_tag="VGR22_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEV2128602.1"
                     /translation="MSEAATSEQQFYYGVGRRKSAVARVRLYPGEGKVTINGKPSKEY
                     FGGREIHLMNVVEPLRATGTGDRFDVVATVVGGGVSGQAGAIRHGVARALVRFDEELK
                     GTLKQAKFLTRDARVKERKKVGLRRARKAPQYTKR"
     gene            complement(13271..13726)
                     /gene="rplM"
                     /locus_tag="VGR22_08300"
     CDS             complement(13271..13726)
                     /gene="rplM"
                     /locus_tag="VGR22_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEV2128603.1"
                     /translation="MERRSYSAKSSTVERSWYVVDAEGKTLGRLATAIATTLRGKHKA
                     SYTPHVDTGDFVIVVNADKVKVTGNKETEKKYYRHSNYPGGLKTTTLRDMRQKHPERI
                     LEAAVRGMLPQTNLGRQQLKKLKLYTTADHPHTAQQPIELDIDDTAARV"
     gene            complement(13793..14602)
                     /locus_tag="VGR22_08305"
     CDS             complement(13793..14602)
                     /locus_tag="VGR22_08305"
                     /inference="COORDINATES: protein motif:HMM:NF013575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HEV2128604.1"
                     /translation="MEPRLKLTVAYDGSRVWGSQRQAGRETVQGILEDALANFAGRPI
                     SAELAGRTDRGVHAAGQVASCEDFRPDLSPDRVRRALEPRLGDTVSVVDIERPPPGFH
                     ARYDARWREYRYRVWAGPRSPLLERYTWHRRGALKVGAMTDAASRLTGMHDFASFVAG
                     GEGVPWSDRQQAARGTVRTVHHCSAREVEPWWPAGEILGTGVEIRVVADGFLPRMVRG
                     FVGALCDIGRGANEPDWIDELKDRADRRGGPRSAPPHGLVLWRIGYGDDRY"
     gene            complement(14621..14974)
                     /gene="rplQ"
                     /locus_tag="VGR22_08310"
     CDS             complement(14621..14974)
                     /gene="rplQ"
                     /locus_tag="VGR22_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEV2128605.1"
                     /translation="MRHRKAGRKFGRNPAQRKAMLRQLAISLIENERLTTTEAKAKEL
                     RSVVEKLVTIAREDSGHHRNVVMSRLDHEPAVARLFDVVAPRYEDVNGGYTRVSKLGP
                     RRGDGAPMVLIEFVA"
     gene            complement(14971..16026)
                     /locus_tag="VGR22_08315"
     CDS             complement(14971..16026)
                     /locus_tag="VGR22_08315"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEV2128606.1"
                     /translation="MEQPQFTIERVEQGLNYGRYVIGPLDPGYGTTLGNSLRRIMLRS
                     LEGVAVSRIRIDNVWHEFATIPNVREDVTEIVLNLKGINLRVVVPFEGELRAHLFYKG
                     DGEGEQTVTAGDISWPGEVEIVNSDHPIATVDGSEAVIDMDLWITSGRGYVTAETQES
                     STLGEIPIDAIFTPIERVNFVVESTRVGQAVDFDRLILEILTNGSIDPEDALTQSAEI
                     LKNHAQVIAEFNQIETAAEPVSELPPEVQNTALADLGLSPRVLNALRSKQIEKVGQVL
                     SMDPEELLSIRNFGPRSLRELRQALQEAGLVPSGEEPAMLEIEDEDPETREAAEAIAS
                     LGSSGEGAETDEEEQDE"
     gene            complement(16122..16754)
                     /gene="rpsD"
                     /locus_tag="VGR22_08320"
     CDS             complement(16122..16754)
                     /gene="rpsD"
                     /locus_tag="VGR22_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEV2128607.1"
                     /translation="MARYTGPVCRLCRREGMKLYLKGARCHTDKCSVAKRSFAPGQHG
                     QRRTRRPSDFGLQLREKQKVRRYYGVLETQFRKHYQEAVRRGGVTGDNLLQILETRLD
                     NVVYRMGFADSRSQARQLVMHGHFTVNGRKTNIPSFAVRPGDVVAVREESRKRDYFKD
                     FGEILATHNPPEWLSVDNSQLSGKVLNLPARDQIEVPLFNDALIVEHYSR"
     gene            complement(16805..17209)
                     /gene="rpsK"
                     /locus_tag="VGR22_08325"
     CDS             complement(16805..17209)
                     /gene="rpsK"
                     /locus_tag="VGR22_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012397714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEV2128608.1"
                     /translation="MAERQRSSTRGRVRRRDRKNIPRGKAYIKATFNNTIVTLTDPAG
                     NVIAWSSAGAHGFKGSRKSTPFAAQVTAEAAARRAQEHGLRQVDVFVKGPGSGREMAI
                     RSLQASGVMVMSIADTTPIPHNGCRPPKRRRV"
     gene            complement(17274..17639)
                     /gene="rpsM"
                     /locus_tag="VGR22_08330"
     CDS             complement(17274..17639)
                     /gene="rpsM"
                     /locus_tag="VGR22_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEV2128609.1"
                     /translation="MARISGVDLPREKRVEVALTYIYGIGLTTSQQLLERTGINPDTR
                     VRDLTDDEANRLREIVDKDLRVEGDLRREVSMNIKRLMDIGSYRGLRHRRGLPVRGQR
                     TRTNARTRRGPKRPVNRRK"
     gene            complement(17688..17801)
                     /gene="rpmJ"
                     /locus_tag="VGR22_08335"
     CDS             complement(17688..17801)
                     /gene="rpmJ"
                     /locus_tag="VGR22_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEV2128610.1"
                     /translation="MKVSASVTKRCERCRVIRRKGIVRVICSNPRHKQRQG"
     gene            complement(18043..18810)
                     /gene="map"
                     /locus_tag="VGR22_08340"
     CDS             complement(18043..18810)
                     /gene="map"
                     /locus_tag="VGR22_08340"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEV2128611.1"
                     /translation="MAITIKNDREIGKMREAGHVVSVCHQRVQASITPGITTDDLNTV
                     VRDTLAEMNATSNFLGHHGFPGVICASVNDEIVHGIPGKRRLNDGDIIAVDIGAIVDG
                     FHGDSAWTYAVGDVSDEAKRLLQDTEDALYAGITVARAGNRIGAIGAAIEAFARPLGY
                     GIVEGYGGHGIGRQMWEEPHIPNLESPDHGPRMRAGMTLAIEPMLNLGNKATRVLDDN
                     WTVVTADGSWSAHFEHTVVVTDGEPEILTKRLASVLH"
     gene            complement(18815..19486)
                     /locus_tag="VGR22_08345"
     CDS             complement(18815..19486)
                     /locus_tag="VGR22_08345"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEV2128612.1"
                     /translation="MNIILMGAQGSGKGTQAAIIAPKLSLEKVATGDLFRAAIADGTE
                     LGVQVKSILERGDLVPDELTNAIVWERLESIAALKAAGELNGALFDGYPRTAGQAEAL
                     DEILARQHDRIDAVIEIQVPRETLIKRLSGRRVCPVCGAVYHIEAEPPRIQGQCDNDG
                     ATLVQRQDDTPEAIRHRLEIYDKQTAPLLAYYDAKDVLSRVDGDQAIDEVTQAILAAI
                     PVKEA"
     gene            complement(19575..20891)
                     /gene="secY"
                     /locus_tag="VGR22_08350"
     CDS             complement(19575..20891)
                     /gene="secY"
                     /locus_tag="VGR22_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEV2128613.1"
                     /translation="MLQAVINAFRIPDLRTKILFTLAMLVIFRFIASVPIPNVDREGL
                     QEYIETNQLLGMLNLFSGSALTNFSIAALGVYPYITASIIMQLMTPIVPRLNELSQEG
                     EQGRNQINKYTHWLTVPLALLQGYGQALLFARQPSPQGGTLIENFGLFDSDTFLPTVA
                     ILATMTAGTMVLVWIGELITEKGIGNGISIIIFGGIVASLPGFVQGLAGTGTTSENII
                     GTVMFLALGLITIVGIVLINEGQRRIPVQYAKRVRQGQMVGGTTTHIPLKVNSAGMIP
                     LIFAVSIMVFPGMVANFLSASNREWVRGLATDISVWLAPTSLPYNIVYFLMVVGFTFF
                     YTMVVFQQQRIPESLQRQGAFIPGVRPGRNTAIFLQRVLSRITIVGALFLGLVAVMPY
                     IASQITGIDTLLLGSTSLLIVVGVAIDTMRQLEAQLMMRNYEGFIS"
     gene            complement(20895..21497)
                     /gene="rplO"
                     /locus_tag="VGR22_08355"
     CDS             complement(20895..21497)
                     /gene="rplO"
                     /locus_tag="VGR22_08355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEV2128614.1"
                     /translation="MPVSIDDLRPDEGAKKNRRRVGRGHGSGRVKTAGRGTKGQNSRS
                     GGGVRPGFEGGQLPIQKRMPYKRGFTNPFKTQWEVVHLSQLEELDVDGPVTPQVLVDA
                     GIVRSLNFPVKILGDGEFSKVITVHAHGFSKSAQQAIEQAGGSIETLEREDRWTGARP
                     RSRRLPLNRELKEMRVGKVGGPSRREALEQLKNRDESKEA"
     gene            complement(21553..21762)
                     /gene="rpmD"
                     /locus_tag="VGR22_08360"
     CDS             complement(21553..21762)
                     /gene="rpmD"
                     /locus_tag="VGR22_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEV2128615.1"
                     /translation="MSEAQTTGGRLRITLVRSVIGRSGDQARTVESLGLRKVNQTVER
                     EDTPSIRGMVTKISHLVKVEEVAGA"
     gene            complement(21957..22466)
                     /gene="rpsE"
                     /locus_tag="VGR22_08365"
                     /pseudo
     CDS             complement(21957..22466)
                     /gene="rpsE"
                     /locus_tag="VGR22_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328273.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
     gene            complement(22517..22891)
                     /gene="rplR"
                     /locus_tag="VGR22_08370"
     CDS             complement(22517..22891)
                     /gene="rplR"
                     /locus_tag="VGR22_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEV2128616.1"
                     /translation="MRTKYRKKLDPRKKRHMRVRAKVSGTAERPRLNVFRSSAHIYAQ
                     LVDDTAGHTILAASDLEDEVQQKAGEDAPKTQRATTVGQVIAERAKAAGIENVVFDRG
                     GFLFHGRVKAVADGAREGGLRF"
     gene            complement(22982..23527)
                     /gene="rplF"
                     /locus_tag="VGR22_08375"
     CDS             complement(22982..23527)
                     /gene="rplF"
                     /locus_tag="VGR22_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEV2128617.1"
                     /translation="MSRIGIKPITVPSGVTVTIGDNNNVTVEGPKGELSSSFSPRMKI
                     SQEDGALTVSRPNDARENRALHGLTRSLLNNMVVGVTEGYTKVLEIQGVGYRAQMQGK
                     NLVLNVGFSHPVTMEPPEGITFGVEANTRVTVSGIDKQAVGEQAAKIRIVRPPEPYKG
                     KGIRYQGEYVRRKAGKAGKAG"
     gene            complement(23577..23975)
                     /gene="rpsH"
                     /locus_tag="VGR22_08380"
     CDS             complement(23577..23975)
                     /gene="rpsH"
                     /locus_tag="VGR22_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEV2128618.1"
                     /translation="MSVIDPISDMLTRIRNAGMARNPETTMPATKVLVAIAGILEQEG
                     YIDGYEVVSKRPQDQLVVRLSYDADRRHAIREIKRVSKPGLRVYAGKDKLPRVKSGLG
                     VAIVSTPEGVLTGYEARRRGIGGEVLCTVW"
     gene            complement(24031..24216)
                     /locus_tag="VGR22_08385"
     CDS             complement(24031..24216)
                     /locus_tag="VGR22_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEV2128619.1"
                     /translation="MAKKSMIVKAQREPKYSTRGVNRCKVCGRPRAYIRKFGTCRICF
                     RELASSGKLPGVTKSSW"
     gene            complement(24235..24774)
                     /gene="rplE"
                     /locus_tag="VGR22_08390"
     CDS             complement(24235..24774)
                     /gene="rplE"
                     /locus_tag="VGR22_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEV2128620.1"
                     /translation="MPRMRERYQEDIVPTLQRELGYQNVYQVPKLEKIVVNVGLGEAI
                     ANGRALEAATNDLATITGQKPVVTRAKKSIASFKLRAGMPIGGMVTLRGDRMYEFLDR
                     LVSVALPRIRDFRGVSPNSFDGRGNYTLGLREQLMFPEIDYDQIDKIRGLEVSIVTTA
                     KTDDEGRRLLALLGMPFAS"
     gene            complement(24899..25222)
                     /gene="rplX"
                     /locus_tag="VGR22_08395"
     CDS             complement(24899..25222)
                     /gene="rplX"
                     /locus_tag="VGR22_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEV2128621.1"
                     /translation="MRKIRTGDEVLVTTGRNKGQQGTVRQNLIDEDRVVVEGVNIVKK
                     HIKRGRARQAGIVEVEAPFHVSNVMLVCPTCKEPTRVGVRKNPEGANERYCKKCDATI
                     PSEQT"
     gene            complement(25231..25599)
                     /gene="rplN"
                     /locus_tag="VGR22_08400"
     CDS             complement(25231..25599)
                     /gene="rplN"
                     /locus_tag="VGR22_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEV2128622.1"
                     /translation="MIQQQTRLKVADNSGARDIMCIRVLGGSGRKFGSVGDTIIASVK
                     VAQPNSAVKKGDVVRAVVVRTAQEYGRPDGSHIKFDDNAAVLINNQGNPRGTRIFGPI
                     ARELREKAFMRIVSLAPEVL"
     gene            complement(25664..25966)
                     /gene="rpsQ"
                     /locus_tag="VGR22_08405"
     CDS             complement(25664..25966)
                     /gene="rpsQ"
                     /locus_tag="VGR22_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEV2128623.1"
                     /translation="MSDVQEAATGTEAAHEQQARQQKVGRVVSNKMDKTAVVAVDYVR
                     RHPLYHKRITRTSKFHAHDENNTCQEGDLVRIEETRPLSKRKRWIVREIVERAARV"
     gene            complement(25983..26198)
                     /gene="rpmC"
                     /locus_tag="VGR22_08410"
     CDS             complement(25983..26198)
                     /gene="rpmC"
                     /locus_tag="VGR22_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003156482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEV2128624.1"
                     /translation="MKAAEIRALNADEREEKLRELYGEWRDLRFQEAVGQMTGTSRIR
                     EIRKDIARIKTIQGEAERTEKSGAAQA"
     gene            complement(26195..26638)
                     /gene="rplP"
                     /locus_tag="VGR22_08415"
     CDS             complement(26195..26638)
                     /gene="rplP"
                     /locus_tag="VGR22_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEV2128625.1"
                     /translation="MLMPKRTKYRKTMRGRMTGKSYRGSTISFGDYGLQALEPSWVTS
                     RQIEAARRAITNYLKRGGKVWIRIFPDKPVTQKPAETRMGSGKGNPEYWVAVVKPGRL
                     LFEVSGVSHEQAEEAMRRAAMKMPMKCKFVAKHTPGATQDEEATA"
     gene            complement(26707..27390)
                     /gene="rpsC"
                     /locus_tag="VGR22_08420"
     CDS             complement(26707..27390)
                     /gene="rpsC"
                     /locus_tag="VGR22_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEV2128626.1"
                     /translation="MGRKVNPIGYRLGIASDWESNWFAERNYTEYLHEDLKIREIIQQ
                     ELTRAGVSRMDIERAANKVDVTLHTSKPGIVIGKQGSNVDRLRKLLERKVGKKVNVKI
                     EEVKAPELDAQLVAESIAEQLQRRVSYRRAVKHAVQQAMRRGAKGVKVRLAGRLGGAD
                     MRREVKEMEGRVPLHTIRAEIDYANVHAHTTYGRIGVKVWVYKGDRLPERELPTEASL
                     TADLAGLAD"
     gene            complement(27390..27731)
                     /gene="rplV"
                     /locus_tag="VGR22_08425"
     CDS             complement(27390..27731)
                     /gene="rplV"
                     /locus_tag="VGR22_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEV2128627.1"
                     /translation="MEVTAKATRVRISPRKARLVVDMVRGKKVNDAIEILKFVPNRAA
                     VDVEKLLKSVAANAENNYDLDPEDLWIKEIYADDAPQMRRYKPKARGRVGRILRRSCH
                     LTVIAEERENA"
     gene            complement(27753..28034)
                     /gene="rpsS"
                     /locus_tag="VGR22_08430"
     CDS             complement(27753..28034)
                     /gene="rpsS"
                     /locus_tag="VGR22_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEV2128628.1"
                     /translation="MSRSSKKGPYIDLKLQKKIDALNSSNQRTVVRTWARASTVFPGM
                     VGHTVAVHNGRQHVPIFITEQMVGHKLGEFAPTRTYRGHAKDADRRGRR"
     gene            complement(28088..28918)
                     /gene="rplB"
                     /locus_tag="VGR22_08435"
     CDS             complement(28088..28918)
                     /gene="rplB"
                     /locus_tag="VGR22_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEV2128629.1"
                     /translation="MPVRKYKPTSPARRSMSVSTFEEITKKKPEKRLIEPLKKHGGRN
                     NRGRITTRHRGGGNKRFYRIIDFKRNKTGIPARVAAIEYDPNRSARIALLFYKDGEKR
                     YMLAPLGIRVDDEVVSGPDAPIRTGNALPLANIPTGTQIHNIELYPGKGGQLVRSAGS
                     AAQLMAKIGDYAQIRMPSGEVRLVHLSCMATLGQVGNVDHENVEIGKAGRSRHMGHRP
                     TVRGSAMNPNDHPHGGGEGKAPIGGQPKTKWGKPAFRRTRNNKRTDNMIVRRRSVKRR
                     "
     gene            complement(28940..29251)
                     /gene="rplW"
                     /locus_tag="VGR22_08440"
     CDS             complement(28940..29251)
                     /gene="rplW"
                     /locus_tag="VGR22_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEV2128630.1"
                     /translation="MAELRLEQVIRRPLITEKNTWLMEKDQYTFEVHPDANKLQIREA
                     VEETFSVKVKAVNTLNVKPKKKSRYVRRGAGRIRGATASWKKAIVTLQPGEHIDLFQQ
                     M"
     gene            complement(29253..29951)
                     /gene="rplD"
                     /locus_tag="VGR22_08445"
     CDS             complement(29253..29951)
                     /gene="rplD"
                     /locus_tag="VGR22_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEV2128631.1"
                     /translation="MQTDILNIQGEKAGSIDLDDTVWGIEPHVSVMHQAVVRQQANAR
                     LGTHDTKTRSEVRGGGRKPWRQKGTGRARHGSIRSPQWKGGGVVWGPHPRKYTQRMPR
                     QMRRLAIRSALSAKMQDGRVSVISDLNNVEPKTKAMKTVLTNLPEARSVLVVMHEKIE
                     AIERAAANLDDVKTVVASMLSVRDLLKYDRVLVTQEAVEVIEGLWALTGEKREPSQWK
                     LERQAARATEEEVA"
     gene            complement(30011..30646)
                     /gene="rplC"
                     /locus_tag="VGR22_08450"
     CDS             complement(30011..30646)
                     /gene="rplC"
                     /locus_tag="VGR22_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEV2128632.1"
                     /translation="MVRGLIGRKLGMTQVFNDEGLAVPVTVIEAGPCVVTQIRTQEKD
                     GYEAVQLGFGIAKKLNKPQAGHVKASGHHVKTLREVKADDYSSIEVGQVFKADTFSEG
                     ELIDITGTSKGRGFQGGVKRHGFRGGPKTHGQSDRQRAPGSIGSSATPGRVFKGLRMA
                     GHMGDERVTVQNLKVVRVDPERNLLLVEGSVPGAKQGTLLIRRAVKVKAAR"
     gene            complement(30818..31141)
                     /gene="rpsJ"
                     /locus_tag="VGR22_08455"
     CDS             complement(30818..31141)
                     /gene="rpsJ"
                     /locus_tag="VGR22_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008933160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEV2128633.1"
                     /translation="MVAARKTASKIRIRLKAYDHKTLDQSAARIVETAESTGAKVAGP
                     VPLPTRIEKFTVLRSPFIDKDSQEQFEVRTHKRLIDVLEPSNSTIRALMKLNLPAGVD
                     IEIKL"
     gene            complement(31223..>31353)
                     /gene="tuf"
                     /locus_tag="VGR22_08460"
     CDS             complement(31223..>31353)
                     /gene="tuf"
                     /locus_tag="VGR22_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV2128634.1"
                     /translation="PGDNVQMGVELITPVAIEEGLRFAIREGGRTVGAGVVTSIDE"
BASE COUNT         5092 a        10804 c         9598 g         5859 t
ORIGIN      
        1 ctgcgccaga ttcagcagcg catcgacgac ctcaatcgcc tgcgcgagga gctccaccag
       61 cacatcggcg acgaggagcc ctccgactga tcctcactca ttgttcggaa cgactgtggt
      121 gcccggcttc tccagcggca cgccgttcgt gcagagcggg cagcgcaccg gctcccacgc
      181 gtcgaccttc atctgcgtca gtgcccgaag cggcacgtcg ccgaaccgca cctcaccgcc
      241 gctgcgatcc accagcacgc cgatccccac cacctcgacg ggatgcgcct tgagcgcggc
      301 gagcgtctcg cgcaccgaac caccagtcgt caggatgtca tccaccacca gcacgcgcgc
      361 accctcgggg aaggtcgtac cgcgccggat ctcccgcttg tcgctaccct ctttcgcccg
      421 ctcggcatag gccgccggca gtcccatctg cctggccgtc tcataggcaa gcaggacccc
      481 gccggttgtc ggtccgacca caacgtcgat atccgtatcc cggaatgcct cgacgaatcc
      541 ggcacagaca tcctcggtcg cgcgcgggtt gcgcagcagg tcgaactttt caaggtagat
      601 atcgctgtga cgcccggacg ccaacaggaa gtggccagtc ttgagcgcac cgatgtcccg
      661 gagctgcgtc atgaggtcca tcactggctg aactcgcttt ccgtgatgag ctgccgaccc
      721 gccgcagccg tctgctgtcg ccgggcgaga tagcgggggt tggccatccc gacgatgtcc
      781 gagagccgcg agtggtcgtg ggctaccagc caggcgggga tgccatcgac cagctccatc
      841 agcaccttgg gctgcgcgaa cgccgccgtg ccgacctgga tcgcgctggc gcccgccatg
      901 aagaactcga tcgcgtcctt gacgctgacg ataccgccca tcccaatcac cgggatggac
      961 accgcaccag ccacctgcca taccatccag acagccaagg ggcgaatcgc cggcccggag
     1021 agtccgccgg tgacgttggc tatgacgggc tccgctcgct cggtgtcgat cgccatgccg
     1081 acgaaggtat tgatcagcga gagtgcgtcc gcccccgcct cctcgacggc ctgagcgatc
     1141 tcgacgacat cgagggcacc cgggctgagc ttgacgatca tcggcatcgt ggtggcagcg
     1201 cgcaccgccc gggtcacctc ggccgacatg tgggcatccc acccgaagca aaatcctccc
     1261 gcgttgatgt tggggcagga gatgttcacc tcaatcgcag ccacgccgga gatgtcatcg
     1321 agccgccggg ccatgtcgac gaagtcctcg acgctctcgg cggagatgtt gacgatcgcc
     1381 ggcacctccc aggattccca gatcggcggg tacgtgcgaa tgaacttcga gatgccagga
     1441 ttctgcaggc cgatcgcgtt gagcatgccg gacggcgtct cggcgatccg gagcattggg
     1501 ttgccggagc gcggcttcgg cgtgatgccc ttgctgacga acgcgccgag tcgctggacg
     1561 tcaatcgcgc cagcgaactc ctgcccgtag ccgaagcagc cagacgccgg aatcaccggg
     1621 ttgcgcagca ccagctcgcg cgggttgccg ggggcgagat cgacagtgac gtcgcgcggt
     1681 gtggttatcc ccatagcagc tcgtccatgt cgaaggcggg gccgtccacg catgcggtct
     1741 tcatgccgtg gcgggtctcg accacgcagc cgaggcaggc gccgatgcca caggccatcg
     1801 gccgctcgac cgccatctgc acgtcggggc gatcgctgag acggttgtgg tggaccacct
     1861 cccgcagcga gcggaacatc ggctccgggc cgcaggagaa gacctggtcg gcccacccga
     1921 ggtactcggc cacgagatcg gtgacgaagc cgctgtgccc ccggctgccg tcgatggtcg
     1981 caacgacgta ctcgacctcg tccggcaact ccgatgcggg gagcagcgcc tcctcattct
     2041 gcccgcccat caggtaggtc acctgcttgc ctcggcgcac cgcctcctcg gccagcatca
     2101 gcagcggcgc ggcaccgacc ccgccggcga ccatcagcag gtggtgggtc ttcggccgca
     2161 cctcgaacgg cctgccgagt gggcccatca catcgagcgg cgtgcccacc acctgctcgc
     2221 agagccacgc gctgccgcga ccatagggcc gcgccagcac cgtgagcgtc ccggcgaccg
     2281 ggtcggcgcg gtagacggag tacgggcggc gcaggagcgg gtcggtcgag cgcggctcgc
     2341 ggctgaggat ctggacgaac tggccgggct tggacgagcg caccgcccac tcgggcgcct
     2401 cgaacgtggt cagcaccgag tcgcccatca cggactcgac atgcacgatg tcgccgtaga
     2461 actccaattt cgtcgcctgt cctgtcgcca ttgagaaccc cattacatcc cggcgcgtac
     2521 gggcggactc cacgtccgtt ccgatgccga ttcagggtcg acatggagtc gacccgtacg
     2581 ggcgatcccc atgatcgcct cctctcctct aatacccgaa cgcgggacgc cgataggaac
     2641 caaccggctc aacggtgaag ctctcgccgg agtgctccat tgcctccacg accgtccgcg
     2701 cggtgtcgat cgaggtaatg catgggatgc cccgctcgac cgccgcccgg cggatctcga
     2761 gcccgtcgag gatctccttg tcagcgggtc ccggggtgtt gatgacgccg tcgacgatcc
     2821 cctcgcggat cacgtccagc atatccggcg agccgtgccc gatgcgcttg gtgaccatct
     2881 gcaccggcat ccctgcccgc tcgatcatcg ccgcggtgcc ctcggtcgcg tagagccgaa
     2941 agcccatccg cgtgagcgcc gaaatgatgt cgagcgactc ggatttgtcc tcgtcggcga
     3001 tcgtgacgag gatcgacccg tgcgccggca gcgagagccc ggccgcgatc agcgccttga
     3061 agaaggcggc atcgaacgag cggtcgatcc ccatgacttc gcctgtcgac ttcatctccg
     3121 gcccgaggtg gatctcgaca ccgcgcagct tggccatcga gaagaccggc gccttcaccg
     3181 ccgtcagcgg ctgccgcggc cagaggccgc ccgcgtagcc ctgctcgcac agcgtcttgc
     3241 caagcatgat gttggtagcg agcttgacca tcggcacgcc cgtcgccttg ctcaggaagg
     3301 gcaccgttcg gctcgatcgc gggttgacct ccagcacgaa gatgcgcccg gcgtggatca
     3361 cgtactggat gttgatcaga ccacgcgcgt tgaggtcctt cgcgatgcgc gtcgtgtagt
     3421 cgacgatcgt ctcgacctcg gccgggtaga gattgacgcc cgggtagacc gcgaacgagt
     3481 cacccgaatg caccccggcg cgctcgatgt gctccatgat gccaggaatc aggatctcat
     3541 cgccgtcgca gatcgcgtcg acctcgacct ccttgcccag caggtacttg tcgaccagca
     3601 ccggatgctc cggcgtcagc gacgcctgat tgcggatgta ccgtgcgagc tggtccgccc
     3661 cgtagacgac ctccatcgcc cgaccaccga gcacgtagga cggccggacc aggacggggt
     3721 agccgatctg cgccgcaacg cgctcggcgt cgtggaggga gatcaccgtc gctccgggcg
     3781 gctgcgggat accgagcgag cgcaggaagg cctcgaagcg ctcgcgatcc tcggcgagat
     3841 caatagtgtc gactgagctt ccccagatcg tcgagcccgc ttcgctcagc ggctcggcga
     3901 ggttgatggc cgtctgacca ccgaactgga gcacgagcgg cgggtagtcc tccagcttgc
     3961 ccttggcctc gtggtagaga atctcctcga cgctctcccg atccagcggc tcgaaataga
     4021 gccggtctga cgcatcgaag tcggtcgaaa ccgtctccgg attggagttg accatgatcg
     4081 actgcacgcc cgcctcgcgg agcgccaacg cagcctgcac cgagcagtag tcgaactcga
     4141 taccttgccc gatgcgaatc ggacctgagc cgacgaccat cgcccggtcg cccttcaggg
     4201 gagccgcctc gtcctcctcc tcgtaagtgg agtaaaagta cggcgtcacg gcctcgaact
     4261 cgccggcgca ggtgtcgacc atcttgtaga ccggcaccat gcccaactcg cgtcgcttct
     4321 ggcggacctc ggcggcggag atgccggcga ggttggcgat ctggatatcg ccgaagccgg
     4381 cctgcttggc ctcccagagc agctccgggg ccaactcgtc gctcgagaga caccgctcca
     4441 ggccaacaag ccgatccagc ttccacagga accacatgtc gatcttgctg agatcgtgga
     4501 tctgctccgg ccgcacgccc cgccgcagcg ccgccatgat ggcccacagt cgcaggtcgt
     4561 tcgggcgctg gatcagctcg tgaatcttct ccggattgtc ttcccaggac ggatcctccc
     4621 agccgaggtc cttcccggtt gtctccagcg accgcaccgc cttctggatc gcgccctcga
     4681 aggagcggtc gatcgccatc acctcgccgg tcgccttcat ttgggttgag atggtgcggt
     4741 cggcgcgcgg gaacttgtcg aacggccacc gcgggatctt ggcaaccacg tagtccagcg
     4801 ccggctcgaa ggctgccgtc gtcttgccgg tgacgacgtt gagcatctcg tcgagtcgct
     4861 tgccgatcgc gatcttggcg ctcaggcgag cgatggggta gccggtggcc ttggaggcca
     4921 gcgctgacga gcggctcacg cgcgggttga cctcgatgac gccgtacttc atcgagttcg
     4981 ggtcgagcgc gagctggacg ttgcagccgc cctcgatctt cagctcccgg atgatcttga
     5041 gcgacgaggt gcgcagcagc tgatattcct tgtcggagag cgtctgcgac ggtgccacca
     5101 cgatgctgtc gccggtatgc acgcccatcg gatcgatgtt ttccatgttg cagatggtga
     5161 tgcaggtgtc ggcaccgtcg cgcatcacct cgtactcgat ctccttccag ccgagaagcg
     5221 agcgctcgag gagcacctgg ccgatcgggc tggcgttgat accagagcga acgtactgct
     5281 ccagttcctg ctcggtgtga gccacgccgc cacccgtgcc gccgagcgtg tacgccgggc
     5341 ggatcaccat cggcagcggc acctcccgtg caaaggcctt tgcctcggcg accgaatgca
     5401 cgatctgacc gggaatcgcc ggctcgccaa tgcgctccag caacgccttg aactgcgagc
     5461 ggtcctccgc catctggatc gactcgaccg gtgtgccgag catccggaca ccatactcgt
     5521 cgagaatgcc gagctcggcg accttcacgg cgagattgag accagtctgg ccgccgatcg
     5581 tcggcagcag cccctccgga cgctcccgct ggatgatgcg ccggatgact tccggcgtga
     5641 gcggctcgat gtagaccgcg tcggcgacat cctcgtcagt catgatcgtc gccgggttgg
     5701 agttcacgag aatggtgcga acgccctctt cccgcagcgc ccggcaggcc tgcgttccgg
     5761 cataatcgaa ctccgccgcc tgtccgatca cgatcggacc ggacccgatg accaacacgc
     5821 tttccggtgt cttcatgtcg ccgctgggtt cccttcgtca gtcgtcggaa gcatgggtcg
     5881 acatggagcc gacccatgta cgggcggtct ccatgaccgc cccagatgct acgccagctg
     5941 tttctgctcg gcggccttgc gctcctcgat catggtgagg aaccgatcga acaggtaacc
     6001 gttgtcctcc gggccgggag aggcctcggg gtggaactgc accgaaagca ccggcagcgt
     6061 ctcgtggcga atcccctcga ccgacccgtc gttcaggttg cgcaggccaa ccagccatcc
     6121 tgcgccttcc gggaaggttc tgccatccac cacgtagctg tggttctgcg aggtgatcga
     6181 gacccgtccg gtctcctcgt cgcgcaccgg gtggttgccc ccacggtgcc cgaacttcag
     6241 cttcgtcgtg ttggcgccga tggcccgcgc gagcaactgg tgaccaaggc agatgccgaa
     6301 gtacggtcgc tggtcctcca gcaggtgctg gaccacgtcg aggcccgtgt cgaggttcgc
     6361 cgggtcaccg ggtcccggcg agagcaccac accgtcagga ctcagggcct cgatctccgc
     6421 gtacggcgta ccgtacggca ccaccgtgac ccgggcaccg cgcgcgacca gcgaccgcac
     6481 gatgttccgc ttgacgccgc agtcgatgac cacgacgtgc ggtccgtcac cggcaccgta
     6541 ggcctcacgc tcgtggccct gcacctcgcc gacgacgtcc tgctcgccgg gcagcttcgc
     6601 cgcgcgagcc cgctcgacca actcctcgtc agtgagatcg gccgcgttgt ggaccagcac
     6661 cgcccgcgta tcgccgaccg agcggatgtg tcgcgtgagc gcccgggtgt cgatgtcgga
     6721 gatgcccgga atgtccgctc gcttcagata gtcatcgatg gtgcctttcg agcgccagtt
     6781 ggacggctgc tcgctagcct cccgcaccac cagaccggcg atccatggcc ggcgcgactc
     6841 gttgtcctcg tcattgacgc cgtagttgcc gatcagcggg taggtcatgc agacgatctg
     6901 cccacggaac gaggggtcgg tgcaaacctc ctggtagccg gtcatcgtgg tggtgaagac
     6961 ggcctcgccg ctggcgtcgg tctcggcgcc gaagcccctg ccgcggaaga cacgaccgtc
     7021 cgcgagcgcc aatgcggcgt cctgaggggg tcgatagcgg tcaggcacgg taagcttcct
     7081 ctccgttgat ccaggtgcga accgccctcc cccgcagggt catgccgatc agcggcgtgt
     7141 tcgcgctctt actccatatg gtatcgcgac tcactaccca cgcttggtct ggatcccaca
     7201 gtgtcacatc cgccggcacg cctgcggtca gggtgccgcg atcgagtccg aaaatctctg
     7261 ccggagctgc cgtccagagg cgcaccaaat cgatggcatc gacgtgcccg gcgcgaacca
     7321 gctcccacgt tgccgggaag gcgaactcga gcccgctcat gccgaatgcc gcccgctcgt
     7381 agggcacgtc cagcttctcc ggatccgcat ggggtgcgtg gtcggtggcg aagatgtcga
     7441 actcgccgcg ctgcaccgcc tccagcagcc cgaccgtgtc ggcgaccggg cgaagcggcg
     7501 ggttgacctt ggtgtagccg tgcgtcggct ccagtggctc gccggccagc gcggtcgtgt
     7561 tgtgcagctc acgcgtgccg gccacccact cgtcgctcat cacgatgtgg tgcggcatca
     7621 cctcggcggt gatgcgcaca ccgcggcgct tcgcctcggt aatcagctcg atgccgcgct
     7681 tcgtgctgac gtgacagacg tgcagccagc cgccggtcag ctcggcaagc atcacatccc
     7741 gggcgatgat gatctcctcg gccgccgcag gaatgccctc gatgccgagc cgccgggaga
     7801 cctcaccctc gtgcatcgcg ccggtggcga gcgccttgtc ctcgcagtga acgatcaccg
     7861 gcacgcccaa ctcaaccgac acttccagcg cctgacgcat gatcgcgctg ttcgcggtcg
     7921 tgtcgccatc gtcggagaaa ccgattgccc ccgcctcaac gagcgccctg aactcgaccg
     7981 gttcggcgcc ggtgcggccc ttcgtgatgg acgcgatcgg caggacacgg caggccccat
     8041 ctcgctcggc ccgctcgatg acgtcccgga tgatttccgg cgaatcgagc gcggggctcg
     8101 tgttcggcat gcacccgccc acgccgaatc caccggcaac cgccgcctgt ccgcccgtcg
     8161 caagggtctc cttgccggtg aagccggggt cgcgcaggtg gacatgcatg tcgatccatg
     8221 caggagtcac cagcttgccg ccggcgtcga tcacctcatc ccgctcacgc ggcttggtgt
     8281 ggtcggcaac ctccgcgacg gtgccgtctt caatgacgac atcagcgacg ccgtcccacc
     8341 cgttcgctgg gtcgatcacc cgcccgccgc gcagtagcgt tcgtctcgtc attggctcgc
     8401 ctcccgtccc gctccgccgt ctctccagta gctgtcaccg cacagcacgc gtaggggtga
     8461 gcccctgcct atactgcctg attcccgaaa aatgcccccg tggtctcgaa gagacccgag
     8521 ggcgcgtgct ctgcagcgtg tatcgttgta ggtgtcaaat tcactgtgtg ccgtgcacct
     8581 cgctcacgtg cgataaaacc aacgaagtat acaggcatcg aacatcgcag tgcaagaagg
     8641 ttggtgatgg cgttctcggc aattgatcac ttggacgagg tcgagcggat tctggacgag
     8701 acctacggtc ctcgcaagct ccggcccagc ggcgacccgg tcggcacgct ggtcagcacg
     8761 atcctcagcc agaacacctc cgacatcaac accgagtgcg cctatgccag tctgcgcgcc
     8821 cgttttccaa catgggatgc ggttgtcaaa gcgaacaccg acgaggtgat cgacgcgatc
     8881 cggtccggag gacttgccaa tcgcaaggct ccgcgcattc aggaggcact ggacgagatt
     8941 cgcgaccgct acggcgaatt ctcgctcgac caactggcga cgatacctct cgaagaggcg
     9001 aaggacacgc tcctctcgat cgacggcgtg ggcccgaaga cagcggcctg cgtgctgctc
     9061 ttcgcgctgg gacggccggc cctgccggtc gacacgcatg tctaccgcgt ctcaaaacga
     9121 ctcggtctca tcgacgatgc caccactccg gtccgcgctc atgcgacact ggaggcgttg
     9181 atggagggcg acgcagaccg cacctaccgc ttccacgtcg agatgattgc ccatggacgg
     9241 acgatctgca ccgcccgcaa cccgaaatgc cacatctgtc cgctcaggag ctactgtgag
     9301 tacgcagcaa cgcacgcgac gtgacccgac cgggcacgaa tccgaccggt ttcagcatcc
     9361 tgtcaaggga gcagtcaccg agctgattgt cgtccggcag gggcagaccg ccgcgaatat
     9421 cgccgggctg ctcgtcggcc gtcaggatgt gctgctgacg gaccttggcc accagcaggt
     9481 agaagcgggt cgccgaggcc gtacacgagc gcgacccgga cgaactcgtc tccagcccac
     9541 tccagcgcgc ccggcagacg accgggccga tcgtagctcg caccgcgctc tacccgaggt
     9601 tcgaggaccg catcgccgag ttcagcttcg gcgacctcga gggatggacc gagcaggacg
     9661 ccctcgatgc ccatcctcac ctgcgcccgc tggtccaaag cgaggcgcac ccagacgagg
     9721 agtggcccaa cggagagtcc ggcaccatct tcctgacccg catcttcgac ggcctgggac
     9781 aagtcgtcgc cgaccacccc gaccagcggg tcagggtggt ggcccatggc ggggtaatcg
     9841 gctcgttcgt ggcacgattg atcgaggaag cgccgacctc cttcttcccc tacctcgtcc
     9901 acaatgccag catctcgacg ttccggatcg acaacgatgg gacacggatt acgttgtggg
     9961 accagcgaca gcatctcgac gggctgccgg aacggtagcg acggagagga gacatgggca
    10021 tgaagctcac gacgatcggc ggcagtgcgg cgggcaccgg taccctgcag ggatgctcgt
    10081 gctacctcgt gcagagcgac accacgagtg tcgtcctcga cctcggatcc gggaccctgc
    10141 aacaactccg cgcgcatgtc gactaccgca cgatcgacgg tatcgtgatc tcccacctgc
    10201 acgcggacca catcgccgac ctggtcgcgc ttcgcttcac cctcagctac aacccggtgc
    10261 ccgcgcctgc tcccgttcct ctctggctcc cgccggacgg catctccaca ctgcggcgta
    10321 tcgcccaagc gttcgagtcg ccggacagcg gcctggagtg gttcaccgac gtcctcgacg
    10381 ttcaggaatt cgatccgaac cgctcgatcg aggtcggcga tctcgtctgc accttcaccc
    10441 ccacggttca cttcgtgccc tgctgggcga tccgggtcca cccggagaat ggcagcggcg
    10501 atctcttcta cgcagctgac accggtccgg cagccgatct cgccggcttc ggcagcggag
    10561 caaaggtggt catcgccgag gcgaccgacg tgggcgaacc cgatctcgac ttcgagtccc
    10621 gtggccatct tacggcggtg gaggcggcgc agctggcaag tgatatcggc gccgaaacgc
    10681 tcgtcctcac ccacatgttc gaggagcatg acccggaggg actgatggct gccgccgagc
    10741 atgttttcga tggcacgctg ttgcgggcgg agccagggct cgaggttcga tggagattct
    10801 gacgagcggc ttccgtacgc tcgacggcgt gcggatcgga aactggacgg accgcgacgc
    10861 acaaaccggt tgtaccgtca tcctgttcga cgcgccggcg ctgaccgctg ccgagattcg
    10921 cggtgcggcg cccggcaccc gcgaggtcga tctgctgcga cccggcatga ccgtccagcg
    10981 tccagacgcg atcctgctca ccggcggcag cgcctttggg ctggctgcag ctgacggcgt
    11041 gatgcggtgg ctgcaggagc gaggacgagg cttccccacg ccagccggcc ccgtaccgat
    11101 cgtgcccgcc gccgtgatct ttgacctcgc catcggcgag ccagtcgcgc ccgatgccac
    11161 ctcggggtac atcgcaaccg acaacgccgt gccgctcgac cagctggctg tcggtcgtgt
    11221 cggggctggt accggcacga cgcacggcag gatcggcggc agcattgcca tccgcgccgg
    11281 tgggttcgct gtcgcgcagg tcgcactgcc ggaggggaca gtgatggcgc tcgtggtcct
    11341 gaacgcctac ggtagcctag cgggagctga cagcgaccct cgggtggcga agctcgatac
    11401 agaagcccac acgccgccct tcggccagtc cacgaccctg ctcgcctgcc tcaccgacat
    11461 cccgctcgac catgcgagtc tccagcgtat gaccgtcgcg atgcacgatg gcctggcgcg
    11521 tgccgtagca ccggtccaca ccgtcgcaga cggcgacatc gcctttgcca caacactgct
    11581 tgaggatcct gtgggaccgg tggaccgtgg cttgcgggtc tctattgctg ccgaactggc
    11641 ggtcgaggca tcgatcggca cacttgccga aggcacctga cacgtcaccg gcatttgcta
    11701 gactgaccgc aaacacgcac cgccgagacg atcttccccg aggctgtcat ggacaccaat
    11761 gccaccggga gcagccggct cccctttcga cgacccacga ttgttgtcgg cacggaatcg
    11821 accctgctcg gccatccgat catcgcgcca aaccaggcta cacaggctgg cttctacgcg
    11881 ctggatctcg atctccggcg cgcatgggac actcggatac cgggcacgct cgccgatcat
    11941 gcaccggcag cgtgtctgcg ctcggcctgg atcccgaggc agtacaccgg cccctggcga
    12001 gaggctcgcg cagtgcatct gcgcgagttc ctcaccgatc tcgtggaatc gtatgggctt
    12061 cgcaccgtca tcgtgccgag cgccgacggg accaggcagc gcgggacctc gcttggacag
    12121 ctcgcccggg aggtgatggc gctcggtggc cttcgggtgg cgatgcgagt cggtccggag
    12181 ttgttgctcg ctcagccagg ctcgcatctc gatcacgttg ccaacctgcg ccgcatggcg
    12241 gaggagtggg atctcgacct cgcactggac ctcaccgctc cgggtatcga agggtgggag
    12301 gccgaagcaa cactgatgcg gctcttcccg cgcctgacca tggtgcgcat ccggcccctg
    12361 cattcgcctg atggcgcacg atcgcaggag ccaaacgcaa ggatcggcct gcggtcgatc
    12421 gctatgctcg ccgatcaggc ctacaacggc gtcatcagca tcgcgccgtc agtctcgccc
    12481 ttgacgcgct ggattccggc gggatcgcgg gtgctggtat ctaccgccct gacacgcgag
    12541 gaagtcctcg cagcttggga ccgcgtcgac cagtacgaca tcaccacgtg gcagaaacgc
    12601 cagcagtttg gatagggacc ggaatccgct cttctcttcg cgccataaca cgatctccgg
    12661 tagtcgcacc caccgcaggc tgcagccctg cacagcaacg atcggcgccc aagggacgta
    12721 gcatccactc ggataaagac cagtcgtcgc caggaagaac aaggaacacc cccggctggc
    12781 aacacaccag ccgggggtgt tctccattcg gtcgccagta cgcgatgcgg tcttaccgct
    12841 tcgtgtactg cggtgccttg cgggcacgtc ggagaccgac cttcttgcgc tcctttacgc
    12901 gggcgtcgcg agtcaggaac ttggcctgct tgagcgtgcc cttgagctct tcgtcgaagc
    12961 ggacgagcgc gcgggcgacg ccgtggcgga tcgcgccggc ctgcccactc accccgccgc
    13021 caaccaccgt cgccaccaca tcgaagcggt cgccagtacc ggtcgctcgg agcggctcca
    13081 cgacattcat caggtggatc tcacggccgc cgaagtactc cttgctcggc ttgccgttga
    13141 tcgtcacctt gccctcgccc gggtagaggc gcacgcgggc gacggcagac ttgcgccggc
    13201 ccacaccgta gtaaaactgc tgttcgctag ttgctgcttc gctcatgctt cctcctttgc
    13261 gcccgtggga ctagacgcga gccgcggtgt catcaatgtc gagctcgatc ggctgctgcg
    13321 ccgtgtgcgg gtggtccgcc gtggtgtaca gcttcagctt cttgagctgc tgccggccga
    13381 ggttggtctg cggcagcatg ccgcgcaccg ccgcctccag aatcctctcg ggatgcttct
    13441 ggcgcatatc acggagggtc gtggtcttca gaccgccggg gtagttggag tgccggtagt
    13501 acttcttctc ggtctccttg ttgccggtca ccttgacctt gtccgcgttg accacgatga
    13561 cgaaatcgcc ggtgtccacg tgcggcgtgt aggacgcctt gtgcttgcca cgcagcgtgg
    13621 tggcaatcgc cgtggcgagt cggccaagcg tcttcccctc ggcgtctacc acataccacg
    13681 agcgctccac cgtgctgctc ttggccgaat acgatcgtcg ttccatcctg tcaaactcct
    13741 gttcggcgtg ccatcaccgt tgctctgcct gatctcgccg tcctggccgg gctcagtagc
    13801 gatcgtctcc gtacccgatg cgccagagca ccaatccatg tggtggtgcc gatcgtgggc
    13861 cgcctcggcg atccgcgcgg tccttcagct catcgatcca gtcaggttcg ttggcgcctc
    13921 gtccaatgtc gcacaaggcg ccaacgaacc cgcgaaccat ccgtggcagg aatccatctg
    13981 ccacgacccg tatctcgacc cccgtcccaa ggatctcgcc cgccggccac cagggctcga
    14041 cctcgcgggc cgagcaatgg tgcaccgtgc ggacggtgcc acgcgccgcc tgctgccgat
    14101 ctgaccaagg cactccctcg ccgcctgcga cgaaggacgc gaaatcgtgc atgcccgtaa
    14161 gacggctggc ggcgtcggtc atcgcgccca ccttgagcgc gccacgccgg tgccaggtat
    14221 accgctcgag gagcggcgac cggggcccgg cccacacccg atagcgatac tcgcgccatc
    14281 gggcatcgta gcgagcatgg aagccggggg gcggtcgctc gatgtcgacc accgaaacgg
    14341 tatcccccag gcggggctcc agcgcccgac gcactcgatc aggactcagg tcgggccgga
    14401 agtcctcaca gctggccacc tgccctgccg cgtggacgcc gcggtcggtt cgtcccgcca
    14461 gttccgcgct gatcgggcgg ccggcgaagt tggcgagcgc atcctccagg atgccctgga
    14521 ccgtctcgcg ccccgcctgc cgttgcgagc cccaaacccg agacccgtca taggcgactg
    14581 tcagcttgag gcgaggctcc accgacgtcg ctccaaatgc ctaggccacg aactcgatca
    14641 gcaccatcgg cgcgccatca ccgcgacgcg gaccgagctt cgaaacgcgg gtgtacccgc
    14701 cgttgacgtc ctcatagcgc ggagcgacca catcgaagag gcgcgccacc gccggctcgt
    14761 gatcgagccg cgacatcacc acattccggt ggtgccccga gtcctcgcgc gcgatggtca
    14821 ccagcttctc gacgacgctg cgcagttcct tggccttggc ctcggtggtc gtcaggcgct
    14881 cattctcgat cagggaaatc gcaagctggc ggagcatcgc cttgcgctgg gccgggttgc
    14941 ggccaaactt gcgtcctgcc ttgcggtgcc tcattcgtcc tgttcctctt cgtcagtctc
    15001 ggccccttcg ccggagcttc ccagcgaggc gatcgcctct gccgcctccc gcgtctcggg
    15061 gtcttcgtcc tctatttcga gcatggcggg ctcctcgccg ctcggtacca aacccgcctc
    15121 ctggagcgcc tgacgcagct ccctcagaga ccggggaccg aagttgcgaa tcgagagaag
    15181 ctcctccgga tccatgctga gcacctgccc gaccttctcg atctgcttgc tccgcagcgc
    15241 gttcagcact cgcggcgaca gaccaagatc ggccagcgcg gtgttctgga cctccggcgg
    15301 cagctcggag accggctcgg cggcggtttc gatctgattg aactcagcga ttacctgcgc
    15361 gtggttcttc agaatctccg ccgactgcgt cagggcatcc tcgggatcga tactcccatt
    15421 ggtcaggatc tcgaggatca ggcggtcgaa gtcgaccgcc tggcccacac gggtgctctc
    15481 gaccacgaag ttgacccgtt cgattggcgt gaagatcgcg tcgatcggga tctccccgag
    15541 cgtcgacgac tcctgcgtct cggcggtcac gtagccgcgc ccgctggtga tccagaggtc
    15601 catgtcgatc acggcctcag agccatcgac cgtggcgatc ggatgatccg aattgacgat
    15661 ctcgacctcg cccggccacg agatgtcgcc ggcggtcacg gtctgctcgc cctcaccgtc
    15721 ccccttgtag aagagatggg cgcggagctc gccctcgaac ggcaccacca cccgaaggtt
    15781 gatgcccttg agattgagca cgatctcggt gacgtcctcc cggacgttcg ggatcgtcgc
    15841 gaattcgtgc cagacattgt cgatgcggat acgggacacc gcgacaccct cgagcgagcg
    15901 cagcatgatg cgccgcagcg agttgccgag agttgtgccg tagccggggt ccagagggcc
    15961 gatgacatac cggccgtagt tgagaccctg ctcgacgcgt tcgatagtga attgcggttg
    16021 ctccaacaag cggctcgcct cctcacgcta tcctgccccg gcattcaccg tcgcggcagg
    16081 tcgcgcccag accgcctgac aggcagtctg gtacacatca attaccggct gtagtgctca
    16141 acgatcaggg cgtcattgaa gagtggcacc tcgatctggt cacgcgccgg caggttcagc
    16201 accttgccgc tgagctggct gttgtccacc gatagccact cgggtggatt gtgagtggca
    16261 aggatttcgc cgaagtcctt gaagtagtca cgcttgcggc tttcctcacg gactgccacg
    16321 acatcgcccg ggcgcaccgc gaacgacggg atgttcgtct tgcggccgtt gacggtaaag
    16381 tggccgtgca tcaccagctg acgggcctgc gagcgcgaat cggcaaagcc catgcggtag
    16441 acgacgttgt ccagccgggt ttcgagaatc tgcagcaggt tgtcacccgt gaccccgcca
    16501 cgccgcaccg cctcctggta gtgcttgcgg aactgggtct cgagcacgcc gtagtagcgg
    16561 cgaaccttct gcttctcccg gagctgcagt ccgaagtccg aagggcggcg cgtgcgccgc
    16621 tggccgtgct gaccgggagc gaacgagcgc ttcgccacgg aacacttgtc agtgtggcag
    16681 cgtgcgccct tgagatagag cttcatgccc tcacgccggc aaagccggca caccgggcct
    16741 gtgtacctcg ccataggttg gtttgagcct cctttaatga atgatgatgg cagtcacgaa
    16801 tgcactagac gcggcggcgc ttcggcggcc gacagccatt gtgcgggatc ggcgtggtgt
    16861 cggcgatcga catcaccatt acgccgctcg cctggagtga ccggatcgcc atctcacggc
    16921 ccgaaccggg acccttgacg aagacgtcga cctgccggag cccgtgctcc tgcgcgcggc
    16981 gggcggccgc ctcggcggtc acttgcgcgg cgaagggcgt gctcttgcgc gagcccttga
    17041 aaccgtgcgc gcccgcgctc gaccaggcaa tcacgttgcc agccgggtcg gtgagggtca
    17101 cgatcgtgtt gttgaatgtt gccttgatgt aggccttccc tcgggggatg ttcttgcgat
    17161 cacgtcgtcg aactcgccca cgagtggaac tgcgctgccg ctctgccatc ctctgccaat
    17221 gcctccatgt atcttcgctg gcgccgggtc actaccgggc tgccgctgtc tgcttacttc
    17281 ctgcgattga ccggccgctt cggaccacgg cgtgtgcgcg cgttggtgcg cgtgcgctgc
    17341 ccgcggaccg gaaggccgcg acgatggcga agaccacggt aggacccgat atccatcagt
    17401 cgcttgatgt tcatcgacac ctcgcggcgg agatcaccct cgacgcgcag atccttgtcg
    17461 acgatctcgc gaagccggtt ggcttcgtcg tcggtcaggt cacgaacgcg ggtgtccggg
    17521 ttgatgccgg tgcgctccag cagctgctgg ctggtcgtta gaccgatgcc gtagatgtag
    17581 gtcagcgcta cctcaacccg cttctcccgc ggcagatcaa ctcccgaaat tctggccatg
    17641 ctctttcctt tgttctcagc ccaacgatat ccgtcaggtc ggagagatca tccctggcgc
    17701 tgcttgtgcc gggggttcga gcaaatcacg cgcacaatcc ccttgcggcg gataacgcga
    17761 caccgttcac atcgcttggt caccgaagcc gagaccttca ttgtgtgtca ctcctctgtt
    17821 gctcaacatt cgaatgcagc aagcgtgatg aacagcaccg cggtgcgagc gtcactgctc
    17881 ggcggccagg ctctcacgtc gcgacccata tgctatgcgt ccatgtcggt tagtctacgc
    17941 ctgtcctgcc atgcggcctc ttgcactgga gaggtgtggt tgcaggagcg acagcgtaca
    18001 cgccgatagc gcatgcctga aatggacatg cgccacgaca gcctagtgta acacgcttgc
    18061 cagtcgtttc gtcaatatct ccggctcgcc gtccgtcacc acgacggtat gctcaaaatg
    18121 tgccgaccac gagccgtccg cagtcaccac cgtccagttg tcatccagca cccgagttgc
    18181 cttgtttccc aggttcagca tcggctcgat cgcgagcgtc atccccgcgc gcatccgtgg
    18241 gccgtggtcc ggactctcga gattcgggat gtgcggctcc tcccacatct gccggccgat
    18301 gccgtggcca ccgtacccct cgacgattcc gtagccgagc ggccgggcga aggcctcgat
    18361 cgccgcgccg atcgccccga tgcgattgcc tgcgcgggcg acggtgatgc cggcatacaa
    18421 cgcgtcctcc gtgtcctgca gcaggcgctt ggcctcatcc gatacgtcac caacggcgta
    18481 ggtccatgcc gaatcgccgt gaaatccgtc cacgatcgca ccgatatcca ccgcaatgat
    18541 gtcgccatcg ttgaggcgac gcttgccggg gatgccgtgg acgatctcat cgttgaccga
    18601 cgcgcagatc acacccggaa agccgtggtg accgagaaag ttggatgtcg cattcatctc
    18661 cgcgagggtg tcgcgcacca cggtgttgag atcgtctgtg gtaatgccag gcgtgatcga
    18721 ggcctgcacc cgctggtggc acacgctcac aacatggccg gcctcgcgca tcttgccgat
    18781 ctcgcgatcg ttcttgatgg taatggccat ggaatcatgc ctccttcaca ggaatcgcgg
    18841 ccaggatcgc ctgcgtgacc tcgtcgatgg cctgatcccc gtcgacgcgg gaaagtacgt
    18901 cctttgcgtc gtagtaggca agcaggggag cggtctgctt gtcgtagatc tcaaggcgat
    18961 gtcggatcgc ctccggcgtg tcatcctggc gctgcaccag ggtagcaccg tcgttgtcgc
    19021 actggccctg aatgcgcggc ggctctgcct caatgtggta gacagcgccg cataccgggc
    19081 acacccggcg accggacagt cgcttgatga gcgtctcgcg cggcacctgg atctcgatga
    19141 cagcgtcgat cctgtcgtgc tgtctggcga gaatctcgtc cagcgcctcg gcctgcccgg
    19201 ccgtgcgagg gtacccgtcg aagagcgcgc cgttcagctc gcccgccgcc ttcaacgcgg
    19261 cgatgctctc caggcgctcc cacacgatcg cgttggtcag ctcatccggt acgagatcgc
    19321 cccgctcgag aatcgacttc acctggacgc cgagctccgt gccgtcggca atggcagcac
    19381 gaaagaggtc gccggtggcg accttctcca gcgacagctt cggcgcgatg atcgcggcct
    19441 gcgtgccttt gccggaaccc tgcgcgccca tgagaataat gttcacgaat atctccagac
    19501 taacggcacg ggtgcgtcca cgatggacgc gccaatgccg ggagcggcac gggtgcgcgg
    19561 ctcccggagt accgtcagct aatgaatccc tcatagttgc gcatcatcag ctgcgcctcg
    19621 agctggcgca tggtgtcgat cgcgacaccg acgacgatga gcagcgacgt cgagccaagc
    19681 agcagcgtgt cgatgcctgt gatctgcgag gcgatgtacg gcatcacggc gacgaggccc
    19741 aggaagagcg caccgacgat ggtgatgcgt gagagcacgc gctgcaggaa gatcgccgtg
    19801 ttgcgcccgg gccgaacacc ggggatgaac gcgccctggc gctggagcga ctccggaatg
    19861 cgctgctgct ggaagaccac catggtgtag aagaaggtga accccaccac catcaggaag
    19921 taaacgatgt tgtacggcag cgaggtcggc gcgagccaca cgctgatatc cgtggccagg
    19981 ccacgcaccc actcccggtt cgacgcagag aggaagttgg cgaccatgcc cgggaagacc
    20041 atgatgctga ccgcgaagat cagcgggatc atgcccgcgc tgttcacctt gagcggaata
    20101 tgcgtggtcg ttccaccgac catctgcccc tgccgcacgc gcttggcgta ctgcaccgga
    20161 atgcggcgct gcccctcgtt gatcagcacg atgccgacga tcgtgatcag gccgagcgcg
    20221 aggaacatga ccgtgccgat gatgttctcg gacgtggtgc ctgtacccgc caggccctgc
    20281 acgaagccgg gcagcgatgc cacgatgccg ccgaagatga tgatggagat gccgttgccg
    20341 atccccttct cagtgatcag ctcaccgatc cagacgagca ccatggttcc agccgtcatc
    20401 gtggcgagaa tcgcgactgt cggaaggaag gtgtcgctgt cgaagaggcc gaagttctcg
    20461 atcagcgtgc cgccctgcgg gctcggctgc cgcgcaaaca gcaacgcctg gccgtagccc
    20521 tgcagcagcg ccagcgggac cgtcagccag tgcgtgtact tgttgatctg gttgcggccc
    20581 tgttcgccct cctgagagag ctcgttgagc cggggcacga tcggcgtcat cagctgcatg
    20641 atgattgacg cagtgatgta cggatacacg ccaagcgccg cgatcgagaa gttggtcaac
    20701 gcgctgcccg agaagaggtt gagcatcccg agcagttggt tggtctcgat gtattcctgc
    20761 agcccctccc gatccacgtt cgggatcggg acactggcga tgaaccggaa gatgaccagc
    20821 atcgccagcg tgaacaggat cttcgtccgc aggtcgggaa ttctgaacgc attgatgacg
    20881 gcctgaagca tcggctaggc ctccttcgac tcgtcccgat tcttcagctg ctccagcgcc
    20941 tctcggcggc tcggaccacc gaccttgccg acacgcatct ccttgagctc acggttgagc
    21001 gggaggcgac ggctgcgcgg ccgagcgccg gtccaacggt cctctcgctc gagcgtctcg
    21061 atggagccgc ccgcctgctc gatggcctgc tgggcggact tcgagaagcc gtgcgcgtgc
    21121 acggtgatca ccttgctgaa ctcgccgtcg ccaagaatct tgaccgggaa gttcaggctg
    21181 cgcacgatgc ccgcgtcgac cagcacctgt ggcgtcaccg gaccatcgac atccagctcc
    21241 tcgagctgcg agaggtgcac gacctcccac tgggtcttga acggattggt gaatccgcgc
    21301 ttgtagggca tgcgcttctg aatcggcagc tgaccgccct cgaacccagg acggacacca
    21361 ccgcctgagc gcgaattctg gcccttggtg ccccgaccgg cggtcttgac gcgaccactg
    21421 ccgtggccgc ggccgacgcg ccgccggttc tttttcgcgc cctcgtcggg acgcagatcg
    21481 tcaatcgaaa caggcatgaa aactctctct atcccgtgcg ctggcgcatg acggggtact
    21541 acggcggcgg gcctatgctc ctgcgacttc ttcaaccttg accaggtggc tgatcttggt
    21601 gaccatgccg cggatcgacg gagtatcctc ccgttccacg gtctggttca ccttgcgcag
    21661 ccccagcgac tcgacagtgc gagcctggtc gccagaccgg ccgataacac tgcggacgag
    21721 cgtgatgcgc agtcgcccgc cagtagtctg ggcctcactc atgacgcatt gctcctctgc
    21781 tgctggccgc cgccgcgacg gttgccaccg ccctggccct gccggcgcgg tcgggagctg
    21841 cgctgctgcg atcgctcgct ctcggcagcc ttggccgcct cgatgcggcg gtcacgggcc
    21901 gcctgcgcgg cggcgaactc gtcgccttgc tcgtgcggct tgcgcaggcg gccggcggcc
    21961 ttcttgcggc gccggatgac atcctgctcg gactgcaggc tctgcagcgc cttcatcgtc
    22021 gccttggtga cgttcaccgg gttgttgttg ccgacgctct tggtcaggac atcacgaatg
    22081 ccggcgagct ccaccacggc gcgcacgccg ccaccagcaa tgacaccggt gcccggcgat
    22141 gccggcctca ggatcacccg ggcggcgccg taggttgact cgacctcatg cgggatggtg
    22201 tgaccgtcca gcggcacggt gaccattgat ttcttggcgg cgtcgacacc cttacggatg
    22261 ctgtccgtca cctcgccgcc cttaccatgg ccgacgccaa cccgaccgtt gctgtcaccg
    22321 acgacaacga tgctgccaaa cgagaaccgg cggccaccct tgaccacctt ggcgacacgg
    22381 ttgatcttga cgagccgctc ttccagctcg ctcccggcat cctcttcccg acggcgcgaa
    22441 ccgcgcccat ctcgattccg atccatacct ctctccagac tgctcgctat cgcggccact
    22501 tggggccgcc acgcggctag aacctgagac cgccctcgcg agctccgtcc gctactgcct
    22561 tgacgcgacc gtggaagagg aatccaccgc ggtcgaagac gacgttctcg atgccggctg
    22621 ccttcgcgcg ctcggcgatc acctgaccga ccgtggtggc gcgctgtgtc ttcggggcgt
    22681 cctcaccagc cttctgctgc acctcatcct cgagatcgct ggccgcaagg atcgtgtgcc
    22741 cagctgtgtc atcgacgagc tgagcgtaaa tgtgggcgct cgagcgaaac acattcaggc
    22801 gaggccgctc ggcggtgccg ctgaccttcg cacgaacacg catgtggcgc ttcttgcgcg
    22861 ggtcgagttt ctttcgatac ttcgtgcgca tgctgttctc cattgatccc ggaactggca
    22921 gacaggccga gtgctgcgcg ttgtgcccgc ccaccaccgg ggcttcaatt cgggttgacg
    22981 gctatccggc cttgccggcc ttgccggcct tgcggcgaac gtactcgccc tggtaccgga
    23041 tgcccttgcc cttgtacggc tcgggcggac ggacgatgcg gatcttcgcc gcctgctcgc
    23101 ccaccgcctg cttgtcgata ccgctcaccg tgacccgggt gttcgcctcc acgccgaacg
    23161 tgatgccttc gggcggctcc atggtgactg ggtgggagaa gcccacattg agcaccagat
    23221 tcttgccctg catctgggcg cggtagccga caccctggat ctcgaggacc ttggtgtagc
    23281 cttcggtcac gcccacaacc atgttgttga gcagcgatcg cgtcaggcca tgcagggccc
    23341 tgttctcacg ggcatcgttc ggtcgcgaga cggtcagcgc gccgtcctcc tgagagatct
    23401 tcattcgcgg cgagaacgag ctggagagct cgcccttcgg cccctcgacc gtcacgttgt
    23461 tgttgtcgcc gatggtcacc gtcacgccgc tcggcacggt gatcggtttg attccaattc
    23521 gagacattgc cctgctctcc cgtcatccgg cagtcgcggg ctccgcacgc ccggggttac
    23581 cagacggtgc agagcacctc gccaccgatt cctcgacgcc gggcttcgta gcccgtcagg
    23641 acgccctcag gggtgctgac gatggcgaca ccgaggccgc tcttgacgcg cggcagcttg
    23701 tccttgccag cgtagacccg aaggcccggc ttgctgacac gcttgatctc gcggattgca
    23761 tgccggcggt cggcgtcgta gctgagccgg accaccagct ggtcctgcgg tcgcttcgac
    23821 accacctcgt acccgtcgat gtaaccttct tgctcgagaa ttcctgcgat cgccacaagg
    23881 accttggtcg ccggcatcgt ggtctcgggg ttccgagcca tgcccgcgtt ccggatccgt
    23941 gtcagcatat cgctgatcgg gtcaatgacg ctcatccgtc gcgttctcca tccttcgcgt
    24001 tcggctgcgg atcgttacgc agtccgagac ctaccaactc gacttcgtca ccccgggaag
    24061 cttgcccgac gaggcgagct cccggaagca gatccggcac gtgccgaact tgcggatgta
    24121 cgcccgcggc cggccacaca ccttgcagcg gttcacgccc cgggtggagt acttcggctc
    24181 ccgctgcgcc ttcacgatca tgctcttctt tgccatgagt cctccagtgc gctcctatga
    24241 agcgaatggc atgccgagca gcgcgagcaa gcggcgcccc tcgtcatcgg tcttcgcggt
    24301 tgtcacgatg ctgacctcaa gcccgcgaat cttgtcgatc tggtcatagt cgatctccgg
    24361 gaacatcagc tgctcgcgaa gaccgagcgt gtagttgccc cgaccatcga acgagttcgg
    24421 cgacacccca cggaagtcgc ggatgcgcgg aagcgccacc gacaccagtc gatcgaggaa
    24481 ctcgtacata cggtcgccgc gcagcgtcac catgccgccg atcggcatgc ccgcgcgcag
    24541 cttgaagctc gcgatcgact tcttcgcccg cgtcaccacc ggcttctggc cggtgatcgt
    24601 cgcgagatca ttcgtggccg cctcgagtgc gcgtccgttg gcgattgcct cgccgagacc
    24661 aacgttgacg acaattttct caagcttcgg cacctgatac acgttctggt agccaagttc
    24721 gcgctgcagg gttggaacga tatcctcctg atagcgctca cgcatgcgtg gcatcgtctt
    24781 gcctcactcc gggccaacgg cccatcctca tctgcagatc ggcgttccca cctggtctgc
    24841 cgccgttcgc ggcactcagg tcgccgcacc tcttaattga tcgttccccg cgggcagatc
    24901 aggtctgctc ggacgggatc gtggcatcac acttcttgca gtagcgctcg ttggcgccct
    24961 cagggttctt gcggacaccg acgcgagtcg gctccttgca ggtcgggcag accagcatca
    25021 cgttggaaac gtggaacggt gcctcgacct cgacgatgcc ggcctgccgg gcgcggcccc
    25081 gcttgatgtg cttcttgacg atgttgacac cctcgaccac tacccgatcc tcatcgatca
    25141 ggttttgacg gacagtgccc tgctggccct tgttccgccc ggtggtgacg agcacctcat
    25201 cgccggtgcg aatcttcctc atcgttactc ctacagcacc tcgggcgcca gcgacacaat
    25261 gcgcatgaac gccttctcgc gcagctcacg ggcgatggga ccaaagatcc gggttccgcg
    25321 tgggttcccc tggttgttga tcagcaccgc cgcgttgtcg tcgaacttga tatggctccc
    25381 atccggccgg ccgtactcct gcgcggtgcg aaccaccacg gcacgaacca cgtcgccctt
    25441 cttgaccgcc gagttcggct gggccacctt caccgacgcg atgatggtgt cgccgacgct
    25501 tccgaacttc cgcccggagc cgccgagcac gcgaatgcac atgatgtcac gcgcaccgct
    25561 gttgtcggcg accttgagtc gagtctgttg ctgaatcacc gttcgtatcc tccgggcaat
    25621 cgctgcccga cgggctctgc gtggggacgg accttgtgtc cgcctagacg cgggccgccc
    25681 gctccacgat ctcccgcaca atccagcgct tgcgcttgga gagcgggcgc gtctcctcga
    25741 tgcggacgag atcgccctcc tggcaggtgt tgttctcgtc gtgcgcgtgg aacttgctcg
    25801 tgcgcgtgat gcgcttgtgg tacagcgggt gacggcgcac gtaatccaca gccaccacgg
    25861 cggtcttgtc catcttgttg ctgacgaccc gaccgacctt ctgctgccga gcttgctgct
    25921 cgtgagcggc ttccgtgccg gtggccgctt cctgaacgtc actcatcgtt accctctatt
    25981 cctcaggcct gcgccgcgcc gctcttctcg gtacgttcgg cctcgccctg aatcgtcttg
    26041 atgcgggcaa tatccttgcg gatctcgcgg atgcgtgatg tacccgtcat ctggcccacg
    26101 gcctcctgga accggaggtc gcgccactca ccgtagagct cgcggagctt ctcctcgcgc
    26161 tcgtccgcgt tcagtgcccg gatctcggct gccttcatgc ggtagcctcc tcgtcctgtg
    26221 tggcaccggg cgtgtgcttc gccacgaact tgcatttcat cggcatcttc atcgccgcgc
    26281 ggcgcatcgc ctcctcggcc tgctcgtggc tcacgccgga gacctcgaac agcaggcggc
    26341 ccggcttcac cacggccacc cagtactccg ggttgccctt gcccgagccc atgcgggtct
    26401 cggccggctt ctgcgtcacg ggcttgtccg ggaagatccg gatccagacc ttgccgccgc
    26461 gcttcaggta gttcgtgatc gcgcggcggg ccgcctcgat ctggcggctc gtcacccacg
    26521 acggctcaag cgcctggagg ccgtaatccc cgaaggaaat ggtcgagccc cggtaagact
    26581 tgcccgtcat gcgtccgcgc atggtcttgc ggtacttggt ccgcttgggc atcaacatgt
    26641 cagatcgctc cgatccctgt tcgaatcggc gtcgcggcga tgccgcccgg ccgatccatc
    26701 cctgtgctag tcggcaagcc cggcgaggtc cgccgtgagc gacgcctctg tcggcaactc
    26761 gcgctcgggg agccgatcgc ccttgtacac ccacaccttc accccaatac ggccgtaggt
    26821 ggtgtgtgcg tggacgttgg cgtagtcgat ctcggcgcgg atggtatgga gcggcacgcg
    26881 gccctccatc tccttcacct cacggcgcat gtccgcgccg ccgaggcggc cagccagccg
    26941 caccttgacg cccttggcgc cccggcgcat cgcctgctgc accgcgtgct tgacggcccg
    27001 ccggtagctg acgcggcgct gcaactgctc tgcaatgctc tcggcgacaa gctgggcgtc
    27061 gagctcggga gccttgactt cctcgatctt gacgttgacc ttcttgccga ccttgcgctc
    27121 cagcagcttg cgaaggcgat cgacgttcga gccctgtttg ccgatcacga tgcctggctt
    27181 cgaggtgtgc agggtgacgt ccaccttgtt ggcggcgcgc tcgatgtcca tgcgcgagac
    27241 accagcgcgg gtgagctcct gctggatgat ctcgcggatc ttcagatcct catgcaggta
    27301 ctcggtgtag ttgcgctctg cgaaccagtt agactcccag tcgctcgcga taccaagacg
    27361 gtacccgatc gggttgactt tacgtcccat taggcgttct ccctttcttc cgcaatgacc
    27421 gtaagatggc acgaccggcg gagaatacgc ccgacgcggc cacgcgcctt gggcttgtac
    27481 cggcgcatct gcggagcatc gtcggcgtag atttccttga tccacagatc ttccgggtcg
    27541 agatcgtagt tgttttcggc gttcgcagcc acggatttca gcagcttctc gacatcgact
    27601 gctgcgcggt tcggcacgaa cttcaatatc tcaatcgcgt cgttcacctt cttgccacgg
    27661 accatgtcga ccacgaggcg agctttccgt ggcgagatgc gaacgcgcgt tgcctttgct
    27721 gtgacttcca tcgctcgtcc tgtccttctc gcctaccggc ggccgcgcct gtcggcgtcc
    27781 ttggcatgac cgcggtacgt ccgggtcggc gcgaactcgc ccagcttgtg gcccaccatc
    27841 tgctcggtga tgaagatcgg cacatgctgg cgaccgttat gcaccgccac ggtgtggccg
    27901 accattcctg ggaagaccgt gctggcgcga gcccaggtgc ggaccacggt ccgctgattg
    27961 ctgctgttga gggcatcgat cttcttctgg agcttcagat cgatgtaggg tcccttcttt
    28021 gaagatcgag acacgttgca ctcctctgtc gagataccga cgcggcgtca gccgccgcgt
    28081 cgaggtccta gcgtcgcttc accgagcgcc ggcgcacgat catgttgtcg gtgcgcttgt
    28141 tgttccgcgt tcggcggaac gccggcttgc cccacttggt cttcggctgg ccgccgatcg
    28201 gggccttgcc ttcaccacca ccatgcgggt gatcgttcgg attcatcgcg gaccctcgaa
    28261 ccgtcggacg gtgccccatg tgtcggctac gacccgcctt gccgatctcg acgttctcgt
    28321 gatcaacgtt gccgacctga cctagcgtcg ccatgcagct caggtgcacc aggcgaacct
    28381 cgccggatgg catgcggatc tgggcgtagt ccccgatctt ggccatcaac tgggcagccg
    28441 atccggccga ccgtacgagt tggccaccct tgcccggata gagctcgatg ttgtgaatct
    28501 gcgtaccggt ggggatgttc gccagcggca gcgcgttgcc ggtgcggatc ggcgcatccg
    28561 gaccggacac cacctcatca tcgacgcgaa tccccagcgg agccagcatg tagcgcttct
    28621 cgccgtcctt gtagaacagc agcgcgatac gggccgaccg gttcgggtca tactcgattg
    28681 ccgcaacgcg agccgggatg ccggtcttgt tgcgcttgaa atcgatgatc cggtagaagc
    28741 gcttgttgcc accgccacgg tgccgggtcg tgatgcgacc ccggttgtta cggccgccat
    28801 gcttcttcag tggctcgatc agccgcttct ccggcttctt cttcgtgatc tcctcgaacg
    28861 tgctgacact cattgagcgg cgcgccgggg aggttggctt gtacttgcga actggcatgt
    28921 ccaatcgttc ccttctgcgc tacatctgct ggaaaaggtc gatgtgctcg cccggctgga
    28981 gagtcacgat agccttcttc cagctcgcgg tggcgccccg gatgcgaccg gcaccacgcc
    29041 gcacgtatcg gctcttcttc ttcggcttaa cgttcaacgt attgacggcc ttcaccttga
    29101 ccgagaaggt ctcctccacc gcctcgcgga tctggagttt gttggcatcc ggatggacct
    29161 cgaaggtata ctggtccttc tccatcagcc aggtgttctt ctccgttatg agcggccggc
    29221 gaatcacttg ttccaaacga agctcagcca tcttacgcaa cctcctcttc cgttgcgcgc
    29281 gcggcctgtc gctccagctt ccactggctc ggctcacgct tctcgccggt cagcgcccag
    29341 agcccctcga tcacctcgac ggcctcctgc gtaaccagca cgcggtcgta cttgagcaga
    29401 tcacgcacgc tgagcattga cgcaacaacc gtcttcacat cgtcgaggtt cgctgcggcc
    29461 cgctcgatgg cctcgatctt ctcgtgcatc accaccagca ccgagcgcgc ctcgggcagg
    29521 ttggtcagca cggtcttcat ggccttggtc ttcggctcga cgttgttcag gtcggagata
    29581 acactcaccc ggccgtcctg catcttcgcc gagagtgccg agcggatcgc caggcgccgc
    29641 atctggcgcg gcatgcgctg ggtgtacttc cgcggatgcg gcccccagac cacgccgccg
    29701 cccttccact gcggcgagcg aatcgagccg tgccgcgcgc ggccggtgcc cttctggcgc
    29761 cacggcttgc ggccaccgcc gcgaacctcg cttcgcgtct tcgtatcgtg cgtgccgagc
    29821 cgcgcgttcg cctgctgacg caccacagcc tggtgcatca ccgatacgtg cggctcgatg
    29881 ccccagacgg tgtcgtcgag gtcaatcgac cccgccttct caccctgaat attcaatata
    29941 tccgtttgca tcgctctgct tgctcccata ccgtcgatct gcatcgacgc aaggtctctc
    30001 gaccggatga ctacctggct gccttgacct tcaccgcgcg gcggatcagc agcgtgccct
    30061 gcttcgcgcc ggggaccgag ccctcgacga ggagcagatt gcgctccgga tcgaccctga
    30121 ccaccttcag gttctgcacg gtcacccgct cgtcgcccat gtggccggcc attcgcagac
    30181 ccttgaacac gcgacccggc gtagcactgg agccgatcga tcccggggcg cgctggcggt
    30241 ccgactggcc gtgggtcttg ggcccacccc tgaagccgtg ccgcttcaca ccaccctgga
    30301 aaccacgacc ctttgaggtc ccagtaatgt cgatcagctc gccctcgctg aaggtgtcgg
    30361 ccttgaagac ctgcccgacc tcgatcgagc tgtagtcatc agccttgacc tcacgcaggg
    30421 tcttgacgtg gtggccactg gccttcacgt ggcccgcctg cggcttgttc agcttcttcg
    30481 cgatgccgaa gccgagctgt accgcttcgt agccgtcctt ctcctgcgtg cgaatctggg
    30541 tcacgacgca cggccccgcc tcgatcacgg tgaccggcac cgccagccct tcatcattga
    30601 acacttgcgt catcccgagc tttcgcccga tcaatccacg aaccattggc tctctccgtt
    30661 gtcaggaact gatggcagcg atgcccgccg caccaggcct gagagcgtac tgtgtgttgt
    30721 ctctccggtt atcggcccgc cagccatacc cccagaatac atccgatggg gtcatctcca
    30781 tgatgacccg tacgggctga ctccatgtcc gcccctttta gagctttatc tcgatgtcga
    30841 caccggccgg gaggttgagc ttcatcaacg cccgaatggt gctgttgctc ggctcgagca
    30901 catcgatcag ccgcttgtgc gtccgcacct cgaactgctc ctgcgagtcc ttgtcaatga
    30961 acggggaccg cagaaccgtg aacttctcga ttcgcgtcgg cagcggcacc gggcccgcaa
    31021 ccttcgcgcc ggtggattcc gccgtctcga cgatgcgagc ggccgactga tcgagggtct
    31081 tgtgatcgta cgccttgagg cgaatccgga tcttcgaagc ggtcttccgc gctgctacca
    31141 tctggtggtt cctcacctct cactcagccg ccgggcgccc gatcatggtc aggcacccgg
    31201 cacctggctc aactcgccgc tgttactcgt cgatggaggt caccacgccg gcgcccaccg
    31261 tgcgcccgcc ctcgcggatc gcaaagcgca gcccctcctc gatcgccacc ggggtgatca
    31321 gctccacccc catctgcacg ttgtcgcccg gca
//
LOCUS       DASYWL010000231        25779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_180052, whole genome shotgun sequence.
ACCESSION   DASYWL010000231 DASYWL010000000
VERSION     DASYWL010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 25779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25779
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180052"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1237
                     /locus_tag="VGR22_08465"
     CDS             <1..1237
                     /locus_tag="VGR22_08465"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV2128635.1"
                     /translation="LYESPLDKSFVEDKGPRQFYGSGLFEYLPARSAASDDATARACV
                     AFSMAELIRSGTTTVMELGSYGDDVAEMATEAGLRAYIGQGYRDGRWFTRDGKRAEWE
                     WDYEAGQQAFRKAVEFIDRVEGKANDRIRGFLTPMQIDTCSEELLKQSRQQATEMGVP
                     LALHASQSVVEFNEMTRRHSLTPIEWLQEIGFLGPDVILGHAIIIGGSTWANYPADDI
                     GILADTGTNVAHAVWVFARRGIAMESFSKYQDRGVNMTLATDTSPQSMLEALRWAAVV
                     SKIVDRSTELATAAHVFNAATIAGARALGRDDLGRITAGAKADMLFWAGQSLWMTPLR
                     DPVRNIVYNAESEDIRHVMIDGQMVMQDRKLANVDEDKVVADLQAAGKKMWSRLPEGD
                     WNNRTVDQLSPNAYKPFEG"
     gene            1244..3067
                     /locus_tag="VGR22_08470"
     CDS             1244..3067
                     /locus_tag="VGR22_08470"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128636.1"
                     /translation="MPSDHTAPAWYQERIDRLTAEHTEVQGRWSLLANVRLVLFVILG
                     VAIWQWWAGGSILAAVIALATAVVLVPLIAFHRRLRGERDRIDRLIRVNTLARQRAAL
                     AWEADLPHTPLPATPPGHPYARDLNMIGEESLLRRIGTPVTVMGWRILQSWLLSPASP
                     EAILERQPAVRELGEDIDLRHEVEQTGIGEELDLDALEELLTWAEAPRLLAARRWLLA
                     LAWIGPLTLATLALAHAFGIIAAPLWVLALAFNLIVTQFLAGDVAVEVDRVGCVSGSL
                     RGYQHIVDRLEEHRPRAPLLRSIHQSLFGGDGNGAGSIRRLTRATAFVIPRGSLLYIP
                     LQMAFAWDLHVAGALDHWKATSGGRLRGWSGTLGEWEALAALGVLVWDHPDWAMPMVD
                     PDDNALRAENLRHPLLPVDIAVGNDVVIGPAGTFLFVTGSNMSGKSTLLRAAGANAVL
                     AQAGGPVAAAAMALPPVDIVTTMRVEDSLAHGVSFFLAELQRLKQVIDAADSARERPV
                     LYLLDEILQGTNTLERQIASRRVLHHLTETTAIGAVSSHDLTLIEGSGLEERAVPVHF
                     SEQFREDSDRPQMTFDYRLRPGLATSSNALKLMQMLGFPEM"
     gene            complement(2989..3903)
                     /locus_tag="VGR22_08475"
     CDS             complement(2989..3903)
                     /locus_tag="VGR22_08475"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEV2128637.1"
                     /translation="MAVVVGGVVLAAIVLLHVVATRVSLRSPRSVQRTLGAIVDPVQH
                     ALSHAVSSHQHYDPADISPYFRVNGRPPESRDYSDLERNGFTGWRLEVCGMVREPQSF
                     SLDDLKAMPRRSSITNHHCIQGWSNIGEWEGVPVSHVLERCRPLPTAQYAVFRALDDK
                     GETMPDIGGKGQYYEVVDLTLLRRSKAILAYEMNGEPLTSAHGAPLRLRLETQLGFKM
                     VKFLAAIEVVEDYREIGEGMGAGVRTPSNTAARPESNIFQAISPCDWFTQRLTRLSYE
                     SPYHISGNPSICISLSALDEVARPGRRR"
     gene            complement(3999..4313)
                     /locus_tag="VGR22_08480"
     CDS             complement(3999..4313)
                     /locus_tag="VGR22_08480"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEV2128638.1"
                     /translation="MGPPGILYVVLLFGTGEWRRLIPTSWDIFPQAWDVLIGYLSFKM
                     PQATAYNRLQQISYAAVVFLLSPLLILTGTAMSPAIAARFPGTRNSLEVPSEHAACTS
                     SA"
     gene            complement(4858..6702)
                     /gene="typA"
                     /locus_tag="VGR22_08485"
     CDS             complement(4858..6702)
                     /gene="typA"
                     /locus_tag="VGR22_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEV2128639.1"
                     /translation="MTDTALATRTDLRNIAIIAHVDHGKTSLVDGLLKQSNIFRDADA
                     AGELIMDSNDLERERGITILAKNTAIMYRDIKINVIDTPGHADFGGEVERVLDMADGC
                     LLLVDAAEGPMPQTRTVLAQALELGLRPIVVINKVDRKNARIDEVLELINDLFLELAV
                     DPEQLEFPVLYAIAKDGRAGFSPDDLQPNLQNLLDTILEVVPAPVVGEEAPFQMRVTS
                     LDYDLHRGRIAIGRVHRGLVSVGEQIAQVAEDGVADRQRVSSLMTFEGLGRREIEIAR
                     AGELVALTGFPDVKIGHTLTHPEHPEPMSKVHIDEPTLKLTFGVSTSPFAGRDGQYST
                     SRQLGERLQRELETNLSLRVEPTGQPDVFAVSGRGELHLSILIETMRREGYEFQVSRP
                     EVITRTLEDGSVVEPVEELTINVPEEFVGTVSELTGARKARMLDMVNDGTGHVRLVYA
                     IPTRGLIGLRSTLLTATKGTATMASRLLEFEPWMGPITSTRSGALVASETGQALTNGL
                     ANAQERGITFIAPGTEVYEGMIVGQNARPDDLVVNVCKGKKLTNIRSSTSDIAVRLTP
                     LTELSLEQCLDFLEDDELLEVTPKVYRLRKRLLTQDARAKERKRATSR"
     gene            complement(7021..7512)
                     /locus_tag="VGR22_08490"
     CDS             complement(7021..7512)
                     /locus_tag="VGR22_08490"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HEV2128640.1"
                     /translation="MTDREQGFRIEEREQSWLTGIAISGPFEIAEAQTNEAWQRLAEM
                     ADAPPNALNRDEWLSACHMRETELTCYVGRTSDGPVGDLPDELVSIRIPRHEYLVARH
                     SGSREELGNLYAAMFAWLHRKDRELNREILWLERFEAPPDPRDDHDLEIWLPLREGAG
                     PRS"
     gene            complement(7509..9239)
                     /locus_tag="VGR22_08495"
     CDS             complement(7509..9239)
                     /locus_tag="VGR22_08495"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEV2128641.1"
                     /translation="MRMAHLFGRTLREAPADAEIPSHQLMVRAAMIRPLGAGLYSLLP
                     LGYRVIRKIEQIVREEMDRIGGQEVLMPVVHPSDIWERSGRWHDVGPEMTRLKDRGGR
                     DLVLAMTHEEVVTDLTATEINSYRQLPQIVYHIQTKWRDEPRARGGLIRVREFTMKDS
                     YTLDTDAEALNTGYEKHAEAYDRIFRRLGLKFIKVGADVGMMGGSKSEEFMAFSPNGE
                     DTLFICPAGDYAANREVAEFRRDEPESEDLLDMELVETPETDTIAKLAELLDIPLSRT
                     AKAVFYIGESGRFIFAVIRGDLDVNETKLRQVTGEGTLVPATADQIRAHGAEPGYGSP
                     VGVKDAVIVVDESVKDSPNLVAGANKPGYHLRNVNLGREYEADVVADIASAWEGAPCP
                     RCGTPMTLERGIEVGNIFQLGTRYTEKMGATYLDKDGKAQPVVMGSYGIGIGRNAAAV
                     VEQCHDERGIIWPVSIAPYHVSLLSLGKDDEVIEATDRLYEDLQAAGIEVLYDDRTDR
                     PGVKFTDAELIGNPIRLSVSSRTLEAGEVELRMRTASESEMAPMDEIVERVKGIVGDL
                     LAELDPDKDA"
     gene            complement(9604..10143)
                     /locus_tag="VGR22_08500"
     CDS             complement(9604..10143)
                     /locus_tag="VGR22_08500"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEV2128642.1"
                     /translation="MRIYTAKGDSGATDLLGERVGKDDPRIGLIGALDESTSAIGLGR
                     AHATIPEWQELLIQAQKDLYQIMAELAFVDELRPNAYRLPRERTEWLEASIADVEATV
                     ELPPQFVLPGDTVAGAALDVARAVVRRAERISVALDHAEQLPNPEILRYLNRLSSLLF
                     IVARAEDLASASGPRLAKS"
     gene            10336..11493
                     /locus_tag="VGR22_08505"
     CDS             10336..11493
                     /locus_tag="VGR22_08505"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2128643.1"
                     /translation="MSWVSRTARYSPLRVDVDLDRPIRVAIIGTGIGAAVHIPVFRQI
                     EETEIVAICSRRAERAKELSDKHRIRIASDDYREIVDHPDVDAVVIATPPYLHHTIAI
                     AAIEAGKHVLCEKPMARNLAEARDMVNLARTAEVAAVVNHEYRYMPARQHVRTMIDEE
                     YIGEPHSASIVVYRSSLADPNGRPFSWLMEREKAGGMLAASGSHYIDLLRWWFGDITA
                     VAGLTATKVEHRHLPGSSEMAHVDADDNFALMLRFQNGGIGTIHVCATAPADAGEEIT
                     LAGSNGLLLLQGDGALWGSRRQDAGLYEVDVPRPRSRKLPEFAHILARPTALLAMDWI
                     EAIRSGEMPEHVPTFEDGARVQEIIDGVHRSSAQGRWIDTSGVRWPVSRGF"
     gene            11695..12825
                     /locus_tag="VGR22_08510"
     CDS             11695..12825
                     /locus_tag="VGR22_08510"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEV2128644.1"
                     /translation="MLATTTAEKTILAADAEVDTPALIVSEEILHNNVSRMASFARSA
                     GVQLRPHIKTHKTPQITRLQIAAGAIGVTCAKVGEAEVMVNEAGAQDVLIAYPTVGAS
                     KYERLVKLMDKARIIQAVDSLAAAKLISKTMSNFDVHMDVVIEVNTGQNRSGVFHGTE
                     AVELAKKIARLPNLHLVGVMTHEGQANSADPADIDRIATQAGEDIVATAEAIRAEGID
                     LEIVSVGSTPAAFITARVKSITEMRPGTYVFNDNSAFRYGHLGVNDMAARFVATVVSR
                     PAPDRAVLDTGSKSLAMDPSKSHPGHGYIVGHPEAVIVKLSEEHGVVLLPDGEAGFEV
                     GDRVEVIPNHICPTVNLMDEMLIVRDGHVVDTWKIAARGKVR"
     gene            complement(12935..14158)
                     /locus_tag="VGR22_08515"
     CDS             complement(12935..14158)
                     /locus_tag="VGR22_08515"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEV2128645.1"
                     /translation="MPRPKILALILAGGRGSRLEPLTEYRAKPAVPYAGVYRLIDISL
                     SNLRNSGISDVGVIVQYETQSIISELANGRPWDLDRTTGGLRVIPPEQRNDRQGPEEV
                     MAHGNADALYENLDVIRAYDADVVLVLSSDHIYRLDYFLPIHEHLERDADATLVTARV
                     PIDDASNYGTCFLDANGRVTGFAYKPEKPESDIVTTEIFVYKPSALSETLERLAKDHE
                     DELEDFGHGLIPELVNAGRVYEYRHNAYWKDVGRPETYFASHMDLLAADSELDLDDPA
                     WPIFTLAPQRMPARIHSSARIAGGLISPGCDIAGTVECSVLAPGVVVEEGATVRNAII
                     LENAVIKSGAEVQYAIVDRYATIGIGAKVGQALGGDQPGRDELALVGQYASVHGTQNV
                     DAGETVEAVDPTRQA"
     gene            complement(14310..18824)
                     /gene="gltB"
                     /locus_tag="VGR22_08520"
     CDS             complement(14310..18824)
                     /gene="gltB"
                     /locus_tag="VGR22_08520"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEV2128646.1"
                     /translation="MEATMRQYAPMYDPRFEHDSCGVGLVVDIHGRQSREIVEKALAG
                     LVNLTHRGGVGADARTGDGAGISLQIPHRFFAEVLQGVGASDFAPGEYGIAMAFLPGD
                     GVAGGKQIIEQALGRRGLPLLGWREVPVDPSVLGDTAAKTLPDIWQAFIARPERLDVD
                     SFEKELMVARRIAEREAAAATMPDFFIASMSARTLIYKGFFLPEDLARFYLDLQDERV
                     ESAIVLFHQRYSTNTFPTWGMAQPFRYIAHNGEINTIQGNRKWMTAREPILRMEGVDT
                     ELITPAVSQHGSDSLSFDNALELLHHGGRTLPRSLMMMVPEPWEQLPEIGPARRAFYD
                     FHAGLIEQWDGPAAIGFSDGVLAGATLDRNGLRPLRYAITRDGLFVAGSEAGTVEIDQ
                     AAVVEKGRLGPGQMILVDTAKGLVLRNDELKENICGQQPYQQWLDEHRVKLDPAPEAD
                     EPLPVPLDADLKTRNALTKTPAEEIALQQAFGYTGEDIRLIVTPMAGEGKEPTWSMGD
                     DAPLALLAERSRPISSFFRQRFAQVTNPAIDSLRERTVMALDAYIGPRGNLLDEVPEQ
                     ARLIHLPSLVLSESMLEAVKANGVLNTIEISTTYRKDAGAGALERELDRIVAEAETAA
                     WNGAGVMVISDRGIDADHVAMPMALVAGAIHNRLIEAGLRMTLDLVADTGEVWDVHQL
                     CVLVGYGVSAVHPWLALRAAAGMSGTRGLEEVTPRQLRENYLVALENGFLKISAKMGI
                     STATGYRGAQIFEIIGLGQEVVDRAFTGTVSQVGGIGFAELEQDLIERHTEAYGQAAK
                     RLPDYGRIKYKRDGEAHGYSPTIAKAIQQASQTHEPQDFQSYLEMVREQPPTTIRDLL
                     GIKPAGDPIPLSEVESEADIIQRFVVTAMSLGALSPEAHKTLSIGMNRIGARSNSGEG
                     GEDPHWYDEPGPDRGHSKVKQVASGRFGVTPRYLSKADELEIKISQGSKPGEGGQIPG
                     HKVTEFIARMRHAVPGMPLISPPPHHDIYSIEDLAQLIYDLRQINPRAKIGVKLVSET
                     GVGTVAAGVAKGRADYILISGHSGGTGASPLASIKHAGSPWELGLAETQQTLVMNGLR
                     SRVKLRTDGGIKTPEDILTAVLLGAEEIGFGTSVLVAVGCDMARQCHLNTCPTGIATQ
                     RAELRAKFTGTPEHVVAWFEQLASHTRAMMAELGIRKLDDVVGCTDLLKREDVAGRAQ
                     CLDLSSLLAEPASEQERRKTLAIDHHAEPTLDMVILEEIAPLLERGESVEVSRGIHTE
                     NRTVGARISGHLTVMRENRPSPLGRVTCTFEGSAGQSFGAFSAPGLRLNLYGEANDYV
                     AKGMGGGQIAIMSPKDATFKEPQAIAGNTILYGATGGTLFIAGSVGERFAVRNSGATA
                     VVEGVGDHGCEYMTGGKVVVLGETGYNFGAGMTNGETWVWDTAGSFAGRINAESVAVR
                     RAAVSELAEVRTLIEQHVAATGSDRGRTILDDWGNQAQAFLRVEPRAALDLARLAVEA
                     ENEAGHGVAD"
     gene            19343..20722
                     /locus_tag="VGR22_08525"
     CDS             19343..20722
                     /locus_tag="VGR22_08525"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEV2128647.1"
                     /translation="MARVAGHLAASQLDRDPEAIHAAIDRIAEHEVRLIDLQFSDIAG
                     GIKALTIPVNLVESTFRRGYRFDGAALSGGRRQVELDLFLAPDPGTLTILPPYDREPR
                     RAQLFCWVLRRDGQPFAGDPRSALQRQLEHAADRGFDYTAGIEMEFYLLSNSDLASTS
                     PLPAADAAGYFDVGEDVLTSTRNEIVDTLATMGIGVGGAHHETGPGQQELDLFPTGGI
                     AIADQIVTTRQVIRTMARRRNLRATFMPKPFPDAPGSGMHVFQQMASLDSGADLLRDG
                     AAADDISDLARRVIAGLLGHAPGMSIIANTTVNSYKRLEAGHRAPRHASWARVSQASL
                     IRVPSALEGAQADIELRSPDAMTNPYLLFASALGAALAGIDEGEEPTSPLDELLVQYD
                     DDEFNRLGVPRLPTTFGEALDTFAADDVIRESVGSYIADQLLHVKKAEWLAYKSHVSP
                     WEHLRYGDL"
     gene            20897..22540
                     /locus_tag="VGR22_08530"
     CDS             20897..22540
                     /locus_tag="VGR22_08530"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2128648.1"
                     /translation="MITVQDVLQQALPATTRVIAGTDHLGREVTWATRLRSAPPGFEH
                     LSGGEVVLLPPRVLEELDERLTLADAIEQLATFQVAAVAVTSNPGARARAVANAAGIP
                     LLVLPRSTDLSSLERQTARFITDQRRAIQHRGQEVTRKLMELAIAGEPLDEQVATLAD
                     LAHRTVVVESRDGRLLASHTPHGDPVSPEVHQLVRQTRDSIGPWLRSVATTSAAEPAT
                     AIFEGANGWRRVVAPVVGRDGLLGSLSLIVDNETGTAADQILTARGAAACAVTLARDQ
                     AAAYARREVELNVLDEILDGALRSEVSLAQQLKRLGHDIEQPHVALIVRAQGEDGVSR
                     ARAGRWSMLEEGISRAASRFGTKSLWRARGSSAEIVWPVSDPTEASQVASALHAEIRD
                     ALARHGVQETVSVGVGRVGSGFDAVRQSHQEARQALMLGRRVRGSGQVTLFDDLGVYR
                     LIYAAEHLPELQGFMAESLGPLMAYDQQHGADLVRTLEAFFEANCSPKETAALLHVHR
                     NTVLYRLDRIASILQADLNDPTIRLRLHLALHVRLALTA"
     gene            complement(22752..23927)
                     /locus_tag="VGR22_08535"
     CDS             complement(22752..23927)
                     /locus_tag="VGR22_08535"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV2128649.1"
                     /translation="MSIELASRMQRLGTETAFEVLMRARALEAQGRDVVHLELGEPDF
                     DTPEHIIEAGAQALHEGWTHYGPAAGQPDLKEAIADYINRSRGTSFSPDQVVVTPGGK
                     PVMFFAILALLEQGDEAIYPDPGFPIYGSMIDYSGAKAVPIPLREENDFRLDIDELTS
                     LVSDRTTFMIINSPANPTGGVLERADLEAIAELAVRHDVVVLSDEIYSELLYEGEHVS
                     IATMPGMAERTIVLDGFSKTYAMTGWRLGYGLFPRELVEPITKLMVNSVSCTSMAVQR
                     AGLAALTGPQDKVAEFREAFRRRRDLIVDGLNAIDGISCVLPKGAFYAFPNITGTGKS
                     SREFADLLLNEHGVAALAGTSFGAAGEGYLRLSYATSEANIQEALNRIAAATTAVGV"
     gene            24202..24933
                     /locus_tag="VGR22_08540"
     CDS             24202..24933
                     /locus_tag="VGR22_08540"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV2128650.1"
                     /translation="MTDASDASTPPRPLIQRRNLGDDVYHILWSRIVSRDLHPGEKLS
                     DVKLSNELGVSRTPVREALQRLLRDGVIETRPHRGFFVRSFSGKDIEEIFEIRAALEA
                     LALRTLVLRSPDEDYGWALKQLDEVAEIIASAGTEEEIVCANERFLEIDQGFHMFLVD
                     RSGNERLKNIINGIWAQIAVFQKAGTHVPRYIEIALRQHREIISLLRKKRHESAIEAL
                     ENHNRVMKERFIADLAEDAREAEAT"
     gene            24930..25145
                     /locus_tag="VGR22_08545"
     CDS             24930..25145
                     /locus_tag="VGR22_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128651.1"
                     /translation="MRPIAWEYHLEPAAVLDADRLNALGREAWELATLVPSTEQAIFK
                     RPALDYRERITLEQRAQVEQGRQEGER"
     gene            25142..25528
                     /gene="rbsD"
                     /locus_tag="VGR22_08550"
     CDS             25142..25528
                     /gene="rbsD"
                     /locus_tag="VGR22_08550"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: protein motif:HMM:NF008761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEV2128652.1"
                     /translation="MKRGGILHPEISRLLASTGHTDYFLVSDRGFPVPPEPERIDLAL
                     VDDIPTVMDVLRAVHAEFVIDRIVIAEEMEQFSPGRVAELRHLMGAVTIEIVSQIELK
                     RLGHDAKATIRTGDTTPYANLIVVSG"
     gene            complement(25525..>25779)
                     /locus_tag="VGR22_08555"
     CDS             complement(25525..>25779)
                     /locus_tag="VGR22_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128653.1"
                     /translation="TRQIRVQYDTPYIRHLPTTLVIGETIGDAYVETVRRPTFKDPYT
                     HELEVFHDVVTGKTTPKTSPEDSMEDLRLFRQIIEVLKQG"
BASE COUNT         4452 a         8633 c         8177 g         4517 t
ORIGIN      
        1 cctctacgaa tcgccgctcg acaagtcctt cgttgaggac aagggaccac gccagttcta
       61 cggctcgggc ctcttcgaat acctccctgc acggtcagcc gccagtgacg acgccaccgc
      121 tcgcgcctgc gttgccttct ccatggccga gctgatccgc agcggcacca ccaccgtcat
      181 ggagctcggt tcctacggtg acgatgtcgc cgagatggcg acggaggccg ggctgcgggc
      241 ctacatcggt cagggctatc gagacgggcg ttggttcacg cgtgacggca agcgtgccga
      301 gtgggagtgg gactacgaag ccgggcagca ggcattccga aaggccgtcg agttcatcga
      361 cagggtcgaa ggcaaggcca acgaccgcat ccggggcttc ctgacgccaa tgcagatcga
      421 tacctgctca gaggagctgc tgaagcagag ccgccagcag gcaaccgaga tgggcgttcc
      481 cctcgcgctg cacgcgtcgc agtccgtggt cgagttcaac gagatgaccc gtcgccacag
      541 cctcacccca atcgagtggc tgcaggagat cggtttcctc ggtccggatg tgatcctcgg
      601 gcacgcaatc atcattggtg gtagcacctg ggccaactac ccggccgacg acatcggcat
      661 cctcgccgac accggcacca acgtcgccca tgccgtctgg gtctttgccc gccgcggcat
      721 cgcaatggag agtttttcga agtatcagga tcgcggcgtc aacatgacgc tcgcgaccga
      781 tacctcgccg cagtcgatgc tggaggcgct ccgctgggct gcggtggtca gcaagatcgt
      841 ggatcgctcc accgagcttg ccacggccgc ccacgtattc aatgctgcta ccatcgccgg
      901 cgccagggcg ctcgggcggg atgacctcgg caggatcacc gcgggcgcca aggctgacat
      961 gctgttctgg gccggacaga gcctgtggat gacaccgctg cgcgacccgg tgcgcaacat
     1021 cgtctacaac gccgagtcgg aagacatccg ccacgtcatg atcgacggcc agatggtcat
     1081 gcaggaccgc aagctcgcca acgtcgatga ggacaaagtg gttgccgatc tgcaggccgc
     1141 cggcaagaag atgtggtctc ggctaccgga aggcgactgg aacaaccgga cggtggacca
     1201 actctctccg aacgcctaca agcccttcga ggggtagtgg ctcttgccgt ccgaccacac
     1261 tgcacctgcc tggtatcagg aacgtatcga tcggctcacc gcggagcaca cagaggtcca
     1321 gggacgctgg agcctgctcg ccaatgtgcg gctagtgctc ttcgtcatcc tcggcgtggc
     1381 gatctggcag tggtgggccg gcggcagtat cctcgcggcg gtcatcgcgc tggcaaccgc
     1441 tgtcgtgctg gtgccgctca tcgccttcca ccgtcggctg cgtggggagc gtgaccgcat
     1501 cgatcgcctg atccgcgtca atacactcgc ccgccaacga gctgcgctcg cgtgggaagc
     1561 cgaccttcca cacacgccgc tgcctgcgac acctccgggg catccatatg ctcgggacct
     1621 caacatgatc ggcgaggagt cgctgctacg ccggatcggc acgccggtca cggtgatggg
     1681 ctggcgcatc ctccagagct ggctgctctc ccctgcgtcg cccgaggcca tcctcgagcg
     1741 ccagcccgcc gttcgggagt tgggcgagga catcgacctc cggcacgaag tcgagcaaac
     1801 cggtatcggc gaggaactcg acctcgatgc gctagaggag ctgctcacct gggcggaagc
     1861 gccgcgcctg ctggccgcac gccggtggct gctcgcgctc gcatggatcg gtccactcac
     1921 gctcgccacc ttggcgctcg cccacgcctt cggcatcatt gccgcgccgc tctgggtgct
     1981 ggcgctcgcg ttcaacctca tcgtcacgca gtttctcgcc ggggacgtcg cagtcgaagt
     2041 cgaccgcgtg gggtgtgtct cgggcagcct acgcggttac caacatatcg tcgatcggct
     2101 cgaagagcac cggccgaggg caccgctctt gcgctccatc caccagtcgc tgttcggcgg
     2161 agatggcaat ggcgcaggga gtatccgccg gctcacccga gcgacggcgt tcgtgatacc
     2221 acgaggatcg ctgctctaca tcccgctgca gatggcattc gcatgggatc ttcacgtcgc
     2281 cggggcgctt gatcactgga aggcgacatc cggtgggcga cttcgcggct ggtccggtac
     2341 gctcggtgag tgggaggcgc ttgccgcgct cggcgtgctc gtctgggacc atcccgactg
     2401 ggcgatgccg atggtcgacc ccgacgacaa cgcgctccgc gccgagaacc tgcggcaccc
     2461 gctgctgccg gtcgatatcg ccgtcggcaa tgatgtcgtg atcgggcccg ccggaacctt
     2521 cctctttgtc acgggcagca acatgtcggg caagagcacc ctgcttcgcg cggcgggcgc
     2581 gaatgccgtg ctcgcccaag caggcggtcc agtcgccgcc gcagcgatgg ccctgccacc
     2641 agtcgatatc gttaccacca tgcgcgtcga ggactcgctg gcgcacggcg tctcgttctt
     2701 cctcgccgaa ctccagcggc tcaagcaggt gatcgacgcg gcagacagcg cgcgggagcg
     2761 gccggtgctc tacctgctcg acgagatcct gcaggggacc aacacgctcg agcggcaaat
     2821 cgcctcgcgc cgggtactgc accacctgac ggagacgacc gcgatcggcg cggtgtcgtc
     2881 gcacgacctg acactgatcg agggcagcgg gctcgaggag cgagcggtgc cggtccactt
     2941 cagcgagcag ttccgggagg acagcgaccg gccacagatg accttcgact accgcctgcg
     3001 gccgggcctc gcgacctcgt ccaacgcact caagctgatg cagatgctgg ggttcccgga
     3061 gatgtgatac ggcgattcat acgacaatcg cgtcaatcgt tgtgtgaacc agtcgcaggg
     3121 ggatatcgct tggaagatat tagattccgg cctcgcggct gtattgctgg gcgtcctcac
     3181 gccagccccc atcccctcgc cgatctcgcg gtaatcttcg accacctcta tggcggcgag
     3241 aaacttcacc atcttgaacc caagctgcgt ctcgagtcga aggcgaagcg gcgctccatg
     3301 ggcggaggtg agtggctcgc cgttcatctc gtaggcgagg atcgccttcg accgccgcag
     3361 caacgtgagg tcgacaacct cgtagtattg ccccttgccg ccaatgtccg gcatggtctc
     3421 gcccttgtcg tcgagtgcgc ggaacacggc atactgcgcg gtcgggaggg gccggcagcg
     3481 ctcgagcaca tggctgaccg gcaccccttc ccactccccg atgttcgacc acccttgaat
     3541 gcaatgatga ttggtgatgc tactgcgcct cggcatcgcc ttcagatcat cgagtgagaa
     3601 ggactgcggc tcccgcacca tgccgcagac ttccagccgc cacccagtga agccgttccg
     3661 ctcgaggtca ctgtagtcgc gactctcagg cgggcggccg ttcacccgaa agtaggggga
     3721 gatatcggcg gggtcatagt gctgatgcga cgacaccgcg tgcgacaggg catgctgcac
     3781 cgggtcgacg atagccccga gcgttcgctg aaccgagcgc ggggagcgca gcgaaacgcg
     3841 agtagctacc acatgcagca ggacgatagc ggcgagaacg acacccccca ccaccactgc
     3901 caccgcgcct tctcccagca agatctgcga aagcccatcc gacagcccgt gaatactgac
     3961 catcgcggtg tgaacgaccg tgaacacacc cacgactatc aggccgagga agtgcaggct
     4021 gcgtgctcgc tgggtacctc caaagagttt cgggtacccg ggaaccgagc agcgattgcc
     4081 ggtgacattg cggtgccagt gagaatcaga aggggcgaga gcaggaagac caccgccgca
     4141 taggagatct gctggagcct gttgtaggcc gtcgcttgag gcatcttgaa ggagaggtag
     4201 ccgatcaaaa cgtcccatgc ttggggaaag atgtcccagg aggtcgggat caggcgtcgc
     4261 cactcgccgg ttccaaacag caggacgacg tagaggatac caggaggacc caagctgccg
     4321 cactggcaaa gtgccagtga cggccgagcc caaggtgcct accacctggc agcgcaacga
     4381 tcggcgagaa cggttgctcc tcttcgtggc tggtccacaa cccgtcgtcc ggcagttgct
     4441 tgtccgtgaa cttcagccac ttactattcg gccgacagtg gttgttccag tagagcttgg
     4501 ggtgcgcact caggatttca atcccactgc gtgccaggag cgacaggaag aggatgttga
     4561 gcaagagcgt gacccggagc caggcaggaa agtcgagcga catgtacctt gtgccctcct
     4621 attggggcac ctactgcaag ttgcctgcca gtccgtgatc tctgccgcac agttttctcc
     4681 gtctcccgat gtgcactttt agagttccgg attttaacca ccagtcgtag tgaaaacctg
     4741 gtagacagaa ggcccggccc gacagacagc tgcaagcgtt acccctcccg gagaacgcat
     4801 aggggcgagc acgctgacgg ggatcgcccc tatgcggcaa tacactgatg acgtcaacta
     4861 ccggctggtg gctcgcttgc gctccttggc gcgggcgtcc tgcgtgagca gccgcttgcg
     4921 aagccggtag accttcggtg tgacctccag cagctcgtca tcctccagga agtcgaggca
     4981 ctgctccagg ctcagctccg tcagtggggt gagacgcacg gcgatatccg acgtgctcga
     5041 ccggatgttc gtcagcttct tgcccttgca gacgttcacc accagatcat ccggccgcgc
     5101 gttctgcccg acgatcatcc cctcgtacac ctcggtgccc ggagcgatga aggtaatgcc
     5161 gcgctcctgc gcgttggcga gaccgttcgt cagcgcctgt cccgtctccg atgcgaccaa
     5221 cgcaccgctg cgggttgagg tgatcggccc catccacggc tcgaactcca gcagccgcga
     5281 cgccatcgtg gccgtgccct tcgtcgccgt gagcaacgtg cttcgcagcc cgatcagacc
     5341 gcgcgtcggg atcgcgtaga ccagccgcac atgcccggtg ccgtcgttga ccatgtcgag
     5401 catccgtgcc ttgcgggcgc cggtcagctc gctgacggtc ccgacgaact cctccggcac
     5461 attgatcgtc aactcctcga ccggctcgac cacgctgccg tcctccagcg ttcgtgtaat
     5521 gacttcaggc cgggatacct ggaactcgta gccctcgcgg cgcatcgtct cgatcaggat
     5581 ggagaggtgc agttcaccgc gccccgagac cgcgaagacg tcaggctgac cggtcggctc
     5641 gacccgcaac gacaggttgg tctccagctc gcgctgaagt cgctcaccga gttgccttga
     5701 ggtcgagtac tggccgtccc ggccggcgaa cggcgacgtg gagacaccaa aggtgagctt
     5761 cagtgtcggc tcgtcgatgt ggaccttgga catcggctcc ggatgctcgg gatgcgtcag
     5821 cgtgtgaccg atcttcacat ccggaaaccc agtcagcgcc accagctcgc cagcgcgcgc
     5881 tatctcgatc tcgcggcgcc ccaacccctc aaaggtcatc agtgacgaca cgcgctggcg
     5941 gtcagcgacg ccatcctcgg cgacttgggc gatctgctcg ccgaccgaga ccaggccacg
     6001 atgcacccgc ccgatggcga tgcgaccccg atggaggtcg tagtcgagcg aggtgacacg
     6061 catctggaag ggtgcttcct cgccgaccac gggtgccggg acgacctcca ggatggtatc
     6121 gagcaggttc tgcagattcg gctggagatc gtccggactg aaaccggccc tgccgtcctt
     6181 ggcgatcgcg tagagcaccg ggaactcgag ctgctccggg tccacagcga gctcgaggaa
     6241 gaggtcgttg atgagctcca gcacctcgtc gatgcgagcg ttcttgcggt cgaccttgtt
     6301 gatcaccaca atcggtcgca gtcccaattc cagcgcctgg gccagcaccg tgcgcgtctg
     6361 cggcatcggg ccctcggccg catcgaccag cagcaggcag ccgtcagcca tgtcgagcac
     6421 gcgctcgacc tcgcccccga agtctgcgtg acccggcgtg tcgatcacgt tgatcttgat
     6481 gtcgcggtac ataatcgccg tgttcttggc gaggatggtg atgccacgct cccgctcgag
     6541 atcgttggag tccatgatca gctcgccggc ggcgtcggcg tcgcggaaga tgttggactg
     6601 cttgagcagt ccatcgacca atgaggtctt gccgtggtcg acatgggcga tgatggcgat
     6661 attgcgaaga tcggtacggg tcgcgagagc ggtatcggtc atgggcgtgg acatccaaag
     6721 gtatcgccca cgatgctatg ggcaagcgag gacaggttgg ttcaggtcga gggagggaca
     6781 cgaaaaggcg ggattcattc gttcccgcgt gttgctccaa ttgaggatac catgatgccg
     6841 attatgtctc ggtactggtg atcgagacgc gccggaaagc tccatgtcat ttcgaccgaa
     6901 gcacccacag ggtgcggagt ggagaaatct ctcgcatgcc ggagtccgac tcccggcagg
     6961 caagaaatct ctcgacacga cgtggctacc tcgagaaccg gcccgaattg acagcaccgg
     7021 tcaggatctt ggtcctgcac cctcccggag gggcagccag atctccaggt cgtgatcgtc
     7081 acgcgggtca ggcggcgcct cgaagcgctc aagccagagg atctcgcgat tgagctcgcg
     7141 gtccttgcgg tgcagccacg cgaacattgc cgcgtagagg ttgccgagct cctcccggct
     7201 accgctgtgt cgcgcgacga ggtactcgtg ccgtggaatc ctgatgctga ccaactcgtc
     7261 gggcaggtcg ccgacgggcc cgtcactcgt cctcccgaca tagcaggtca gctcggtctc
     7321 ccgcatgtgg catgcgctca gccactcatc acggttgagc gcattgggcg gtgcatcggc
     7381 catctctgcg agccgctgcc atgcctcgtt ggtctgcgcc tcggcgatct cgaacggacc
     7441 agagatggcg atccctgtca accaggactg ctcccgctcc tcgatccgaa acccctgctc
     7501 gcgatccgtc atgcgtcctt gtccgggtcc agttcggcga ggaggtcgcc gacgatcccc
     7561 tttacccgtt cgacgatctc atccatcggc gccatctcgg actcgctcgc cgtccgcatt
     7621 cgcagctcga cctcgcccgc ctccaatgtg cgggagctga ccgagaggcg gatcgggttg
     7681 ccgattagct cggcgtcggt gaacttcacc cccggtcggt cggtgcggtc gtcgtagagg
     7741 acctcgatcc cagctgcctg gagatcctcg tagagcctgt cggtcgcctc gatcacctcg
     7801 tcatccttgc cgagcgagag cagcgagaca tggtaggggg cgatcgacac cggccagata
     7861 atgccgcgct cgtcgtggca ctgctcgaca accgcggcgg cgtttcgccc gatgccgatg
     7921 ccgtaggagc ccatcaccac cggctgcgcc ttgccatcct tgtcgaggta ggtcgcgccc
     7981 atcttctcgg tgtagcgagt gccgagctgg aaaatgttgc cgacctcgat gccgcgctcc
     8041 agcgtcatcg gagtaccgca cctggggcac ggcgcaccct cccaggcaga ggcgatgtcg
     8101 gcgacgacat ctgcctcgta ttcgcggccg aggttgacgt tcctcaggtg gtagcctggc
     8161 ttgttggcac cagcgacgag gttcggggag tccttcacgc tctcgtcgac gacgatgacg
     8221 gcatccttca cgccaaccgg tgagccgtag ccgggctcgg cgccgtgtgc gcggatttgg
     8281 tcagccgtcg ccgggacaag ggtgccttcg ccggtgacct ggcgcagctt ggtctcgttg
     8341 acatcgaggt cgccgcgaat cactgcgaag atgaagcggc cgctctcgcc gatatagaag
     8401 acggctttcg ccgtgcggct cagcgggatg tcaaggagtt cggccagctt ggcaatggtg
     8461 tccgtctccg gcgtctcgac cagctccata tcgagcagat cttcgctctc cggctcgtcg
     8521 cgccggaact ctgccacctc ccgattggcg gcatagtcgc cagctgggca gatgaagagc
     8581 gtgtcctccc cgtttggcga gaaggccatg aactcctccg acttcgagcc gcccatcatg
     8641 ccgacatcag cgccaacctt gatgaacttc agcccgagtc gcctgaagat gcggtcgtag
     8701 gcctcggcgt gcttctcgta accggtgttg agcgcctcgg catcggtgtc gagcgtgtag
     8761 ctgtccttca tcgtgaactc acgcacgcgg atgagccctc cccgggcgcg cggttcgtct
     8821 cgccatttcg tctggatgtg gtagacgatc tgcgggagct gccggtagga gttgatttcg
     8881 gtggcagtaa gatcggtgac gacttcctcg tgggtcatcg ccagcaccag gtcgcggccg
     8941 ccgcggtcct tgaggcgggt catctccgga ccgacgtcgt gccagcggcc cgaccgctcc
     9001 cagatgtcgg aaggatggac gaccggcatc agcacctcct gcccgccgat gcggtccatc
     9061 tcctcgcgga cgatctgctc gatcttgcgg attacgcggt atccgagggg caagaggctg
     9121 tagaggccag cgccgagggg gcggatcatc gctgccctga ccatcagctg atggctaggg
     9181 atctccgcgt ccgccggtgc ctcgcgtagc gttcgtccaa agagatgcgc catccgcatg
     9241 gttgtcgtcg tcctccgtat ggatggtctg gtggcccgcc cgttgatacc gcacagcttc
     9301 gtcacaatcc cccacaggat acagtacagg aaagtcgcca ctcggcaggc tgggatcata
     9361 cgagaacatc ggcgattcct ctctggtaga accagttcgt tatcgtaggg gacgccttgg
     9421 ctccgcggaa cgttgttttg gccgatgatc gccgggcgca gccaagacgt cccggtgttg
     9481 tgattcgggc aggaatcggg aggacattga ccgcggggcc cggcgtttcc catggcgttg
     9541 gcaagcacgg tcaattctcc cctaccgctc cacgcgggcg ctcatgtgca cgcgccacac
     9601 cgatcaggac ttcgccagac gggggccact ggcgctggcg aggtcctcgg ctcgggcaac
     9661 gatgaagagc agtgacgaga gccggttgag atagcgcagg atctccgggt tgggcagttg
     9721 ctcggcgtgg tcgagcgcca ctgaaatccg ctccgctcgg cgcacgaccg cccgggcaac
     9781 gtccagcgcc gcaccggcga cggtgtcccc tggcagcacg aactgcggtg gcagctctac
     9841 cgtcgcctcg acgtccgcga tcgacgcctc cagccactcc gtacgctccc tcggcagccg
     9901 gtaggcattc ggtcgcaact catcgacgaa ggcgagctct gccatgatct ggtagagatc
     9961 cttctgcgct tggatcagca gctcctgcca ttcagggatg gtcgcgtgcg cccttccgag
    10021 cccgatcgca gatgtcgact cgtcgagggc accgatgagg ccgatgcgcg gatcgtcctt
    10081 gccgacgcgc tctcccagca ggtcagtggc accgctgtcg cctttggctg tgtaaatccg
    10141 catccctgct ctctcctgca gcgtgattcc gtagccattc gagggccact cggccggtac
    10201 gggggtgtcc gtatagtgtg gtagcctagg ccccacggtg ggaagtctgg cacgcgctcc
    10261 aaccatcttc cacgacggca ccgccgatca ccgatgtgcc ttcctgttgc cgtcgcccgt
    10321 gcgaggtgca tgctcgtgtc ttgggtttcg cgaacagcac gctactcccc gctccgcgtg
    10381 gacgttgatc ttgaccggcc gatccgcgtc gccatcatcg gaacgggcat cggcgccgcc
    10441 gtccacatcc cggtcttccg ccagatcgag gagacggaga tcgtcgcgat ctgctcgcgc
    10501 cgcgctgagc gggcgaagga gctttccgac aagcaccgca ttcggatcgc ctccgatgac
    10561 taccgggaga tcgtcgacca ccccgacgtc gacgcggtcg tgatcgccac cccgccctat
    10621 ctccaccaca ccatcgcgat cgccgccatc gaggccggca agcatgtgct ctgcgagaaa
    10681 ccgatggcgc gcaatctcgc cgaggcccgc gacatggtta accttgcgcg cacggcagag
    10741 gtcgccgctg tggtcaacca cgaataccgc tacatgccgg cgcggcagca cgtgaggacg
    10801 atgatcgacg aggaatatat cggcgagccg cattcggcgt cgatcgtggt ctaccggtcc
    10861 agcctcgccg accccaacgg ccgtcccttc agttggttga tggagcgcga gaaagccgga
    10921 ggcatgctcg cggcctccgg ctcccactac atcgaccttc tccgctggtg gttcggtgac
    10981 atcaccgccg tcgcagggct gactgccacg aaggtcgagc accggcacct gccaggctca
    11041 tctgagatgg cgcacgtcga cgccgacgac aacttcgccc tgatgctgcg cttccagaac
    11101 ggtgggatcg gtacgatcca cgtctgcgcg actgcacccg ccgatgccgg cgaggagatc
    11161 acccttgcgg gatcaaatgg cctgctgctg ttgcagggcg acggggcgct atggggctcg
    11221 cgccgacagg acgccggcct ctacgaggtg gacgtgccac gaccccgcag ccgcaagctg
    11281 ccggagttcg cgcacatcct tgcgcgcccg accgccttgc tggcgatgga ctggatcgag
    11341 gcgatccgta gcggcgagat gcctgagcac gtaccgacct tcgaggacgg cgcccgcgtg
    11401 caggaaatca tcgacggcgt gcaccgctcc tccgcccagg gccgctggat cgacaccagt
    11461 ggtgtccgct ggcccgtctc ccgcgggttc tgatacctcc atcgccgtca cctgacggca
    11521 gcaacgaccg aggcgcagcg gcgggcctcc cgaatgagtc atccgcgcat ccgccggcag
    11581 ggccgggccc ccgcctcgaa ccagccgcct ttgcctgcga acgcaccgaa aaccgtgtac
    11641 catgagcatc cgtcaaatcc gtgaggcccg caccaacagg aggctgagtt cgacatgctc
    11701 gcaaccacca ccgccgagaa gacaatcctc gctgccgacg ccgaggtcga cacccccgcc
    11761 ctgatcgtct cggaagagat cctgcacaac aacgtctccc gcatggcatc gttcgcgcgg
    11821 agcgccggcg tgcagctgcg tccacacatc aagacccaca agaccccaca gatcacccgg
    11881 ctgcagatcg ccgccggcgc cattggcgtg acctgtgcca aggtcggcga ggcagaggtg
    11941 atggtcaacg aggccggcgc ccaggacgtg ctgattgcct acccgacggt gggcgccagc
    12001 aagtacgagc ggctcgtgaa actgatggac aaagcccgca tcattcaggc ggtcgactcg
    12061 ctcgccgcgg cgaagctgat ctcgaagacg atgtcgaact tcgacgtcca tatggacgtg
    12121 gtgatcgagg tcaacaccgg ccagaaccgc agtggcgtgt tccacggcac agaggcggtc
    12181 gagctggcga aaaagatcgc ccggctgccg aacctgcatc tcgtcggcgt catgacgcac
    12241 gagggtcagg ccaacagcgc cgaccccgcc gatatcgatc ggatcgccac ccaagcgggt
    12301 gaggacatcg tcgccacggc ggaggcaatc cgcgccgagg gcatcgacct ggagatcgtc
    12361 agcgtcggct ccactcccgc ggcgttcatc actgcgcgag tcaagagcat caccgagatg
    12421 cgcccgggca cctacgtctt caacgacaac tcggccttcc ggtacggtca cctgggcgtc
    12481 aacgacatgg cagcgcgctt cgtcgcaacc gtagtcagcc gtcccgcccc cgaccgcgcc
    12541 gtgctcgaca ccggctcgaa atcgctggcg atggacccga gcaagagcca cccagggcat
    12601 ggctacatcg tcggacaccc ggaagcggtc atcgtgaagc tgagtgagga gcacggtgtc
    12661 gtcctgttgc cggacggcga ggcgggcttc gaagtcggag accgtgtcga ggtcatcccg
    12721 aaccatatct gcccgactgt gaacctgatg gacgagatgc tgatcgtccg tgacggccac
    12781 gtggttgaca cctggaagat cgccgcccgc ggcaaggtcc ggtagacaga tcgtatccgg
    12841 gactgattgc agcgaaaaac gggcggacat cgagttcgcc cgcaccggtc gaccccatgt
    12901 cggcccttct tcatgcttgg cggcataccc gtcgtcacgc ctgccgcgtg gggtcgacgg
    12961 cttcgacggt ctctccggca tccacgttct gggtgccatg caccgaggcg tactgcccca
    13021 cgagtgcaag ctcgtcccga ccgggctgat cgcctccgag tgcctgcccg accttcgcac
    13081 cgatcccgat cgtcgcgtag cggtcgacga tggcgtactg cacctcagcg ccagacttga
    13141 tgaccgcatt ctccaggatg atcgcgttgc ggaccgtcgc cccctcctcg acgacgacac
    13201 cgggagccag cacgctgcac tcgaccgtgc cggcgatgtc gcagccaggc gagatgagcc
    13261 ctccggcgat ccgtgccgaa ctgtggatgc gagccggcat gcgctgaggc gcgagcgtga
    13321 agatcggcca cgcggggtcg tccaggtcga gctcggagtc ggcagcgagc agatccatgt
    13381 gtgaggcgaa gtaggtctcc ggacggccga catccttcca gtaggcgtta tggcggtact
    13441 cgtagaccct ccccgcgttc accagctcgg gaatcagacc gtgcccgaag tcctccagct
    13501 cgtcttcatg gtccttggcc aggcgctcca gcgtctcgct gagggccgaa ggcttgtaca
    13561 cgaagatctc ggtcgtgacg atgtcgctct cgggcttctc cggcttgtag gcgaagccag
    13621 tgacacgtcc attcgcatcc agaaagcagg tgccgtaatt cgaggcgtcg tcgattggca
    13681 cgcgggccgt cacgagggta gcgtcggcat cccgttcgag gtgctcgtgg atgggcagga
    13741 agtagtcgag ccggtagatg tgatccgagc tgagcaccag cacaacgtcc gcgtcgtagg
    13801 cccggatgac atccaggttt tcgtagagcg cgtcggcatt cccgtgagcc atcacctcct
    13861 ccgggccctg ccggtcgttg cgctgctccg gcgggatcac gcgcagcccg ccggtggtgc
    13921 gatcgagatc ccatggccgg ccgttggcga gctcgctgat gatcgattgg gtttcgtact
    13981 ggacgatcac gccgacgtcg ctgataccgc tgttgcgtag gttgctgaga gagatgtcga
    14041 tgaggcggta gacgccggcg tagggcacgg caggcttggc acggtactcg gtaagtggct
    14101 ccagacgact gccgcgccca ccggcgagga tgagcgcgag gatcttcggt cgtggcatgt
    14161 ggtcggctcc cctgtttcga tcgcattcaa tcgggaaccg gggatgcaag cgctgtgcca
    14221 gccgaataga gaaagggcgg acatggagcc cgcccgtacg ggtcgactcc atgtcgaccc
    14281 cttcattccg attgtgttga cccaggcggt cagtctgcga caccgtggcc ggcctcgttc
    14341 tcggcctcga ccgccagccg ggcgaggtcc agcgccgcac gaggctcgac tctcaagaac
    14401 gcctgcgcct ggttccccca gtcgtccagg atcgtccggc ctcggtcgct ccccgtcgcg
    14461 gcgacgtgct gctcgatcag cgtccgcacc tcagccagct cactgacggc cgctcgccgc
    14521 accgccacgg attcggcatt gatgcgaccg gcgaacgaac ctgccgtgtc ccagacccat
    14581 gtctcgccgt tggtcatgcc cgcgccgaag ttgtagccgg tctcgcccag caccacgacc
    14641 ttgccaccgg tcatgtactc gcagccgtga tcgcccacac cctcgacgac cgccgtcgcg
    14701 ccgctgttgc ggaccgcgaa gcgctcgccc accgagccgg cgatgaagag cgttcctccc
    14761 gtcgcgccgt agaggatcgt gttgccagcg atcgcttggg gctccttgaa ggttgcgtcc
    14821 ttcggcgaca tgatggcgat ctggccccca cccattcctt tggcgacgta gtcgttggcc
    14881 tcgccgtaga gattcagccg cagccccggc gcgctgaacg ccccgaagct ctgcccggca
    14941 ctgccctcga aggtgcaggt cacgcgcccg agcggcgagg gccggttctc gcgcatcacc
    15001 gtcagatgac cggagatgcg cgcgccgacg gtgcggttct cggtatggat cccccggctg
    15061 acctcgaccg actcaccgcg ctccagcaat ggcgcgatct cctccaggat caccatgtcg
    15121 agcgtcggct cggcatggtg atcgatcgcg agtgtcttgc ggcgctcctg ctccgaggct
    15181 ggctccgcga gcaggctgct gaggtcgagg cattgggcgc gtccggcaac atcctcccgc
    15241 ttgagcaggt cagtgcaccc gacgacatca tcgagcttgc ggatgccgag ctcggccatc
    15301 atcgctcgcg tgtgcgaggc gagctgctcg aaccacgcca ccacgtgctc cggcgtgccg
    15361 gtgaacttcg cccgcagctc ggcgcgctgg gttgcgatgc cggtcgggca ggtattcagg
    15421 tggcactggc gcgccatatc gcagccgact gcgaccagca ccgaggtgcc gaagccgatc
    15481 tcctctgcac cgagcagcac cgccgtgagg atgtcctccg gcgttttgat gccgccgtcg
    15541 gtgcgaagct tcacgcgcga tcgcaggccg ttcatcacca gcgtctgctg ggtttcggcc
    15601 aggccgagct cccacgggct accggcatgc ttgatcgacg ccagcggcga ggcgccggtg
    15661 ccgcccgaat gcccgctgat gaggatgtaa tcggcgcgac ccttggcgac accggcggcg
    15721 acggtgccga cgccggtctc ggagacaagc ttgacgccga tcttcgcgcg agggttgatt
    15781 tgtcgcaggt catagatgag ctgtgcgaga tcctcgatcg agtagatgtc gtggtgcggc
    15841 ggcggcgaga tcagtggcat gccgggcacc gcatggcgca tgcgcgcgat gaactcggtc
    15901 accttgtggc cggggatctg gccgccctcg ccgggcttcg aaccctggga gatcttgatc
    15961 tccagctcgt cggccttcga gaggtagcgc ggggtgacgc cgaagcgccc cgaggcgacc
    16021 tgcttgacct tggagtgtcc gcggtccggc cctggctcgt cgtaccagtg cggatcctcg
    16081 ccgccctcgc cagagttaga gcgcgcgccg atgcggttca tgccgatcga gagcgtcttg
    16141 tgcgcctcgg gactcagtgc accgagcgac atcgccgtga cgacgaagcg ctggatgatg
    16201 tccgcctccg actcgacctc actcagcggg atcgggtcgc cggccggctt gatgccgagc
    16261 aggtcgcgaa tcgtggtcgg tggctgctcg cgcaccatct cgaggtagct ctggaagtcc
    16321 tgtggctcgt gggtctggct cgcctgctgg atcgccttcg caattgtcgg cgagtagcca
    16381 tgtgcctcgc catcccgctt gtacttgatg cggccgtagt ccggaaggcg cttcgccgcc
    16441 tgtccgtaag cctcggtatg gcgctcgatc aggtcttgct caagctcggc gaacccgatg
    16501 ccgccgacct gcgagacggt gcccgtgaat gcccggtcaa cgacctcctg gccgaggccg
    16561 atgatctcga agatctgcgc gccccggtag ccggtcgcgg tcgagatgcc catcttcgcg
    16621 gagatcttga ggaaaccgtt ctccagtgca acgaggtagt tctcccgcag ctggcggggc
    16681 gtcacctcct cgagcccacg cgtgccggac atgccggccg ctgcccgaag agccagccac
    16741 ggatgcaccg ccgagacacc gtagccaacc agcacgcaga gctggtggac gtcccagacc
    16801 tcgccggtgt cggcgacgag atcgagcgtc atgcgaagcc cggcttcgat gagccggttg
    16861 tggatcgctc cggcgacgag cgccatcggc atcgccacgt ggtcggcgtc gatcccgcgg
    16921 tcgctgatga ccatcacacc ggcgccgttc caggcggctg tctccgcctc ggccacaatc
    16981 cggtcgagct cccgctcgag cgctccggcc ccggcgtctt tccggtacgt ggtcgagatc
    17041 tcgattgtgt tgaggacgcc gttggccttg acggcctcca gcatcgactc gctcagcacg
    17101 aggctcggca ggtggatgag gcgcgcctgc tccggcacct cgtcgagcag gttgcctcgc
    17161 ggaccgatgt aggcgtccag cgccatcacc gtgcgctcgc gcagcgagtc gattgccgga
    17221 ttggtcacct gtgcgaagcg ctgccggaaa aaggacgaga tcggtcgcga ccgctcggcc
    17281 agcaaggcca agggagcatc atcgcccatc gaccacgtcg gctccttacc ctcgcccgcc
    17341 attggcgtca ctatcaggcg gatatcctcg ccggtgtagc cgaacgcctg ctgcagcgcg
    17401 atttcctccg ccggggtctt ggtcagtgcg ttgcgcgtct tcaggtcggc gtcgagcggc
    17461 accggcagcg gctcgtccgc ctcgggggcg gggtccagct tcacacggtg ctcgtccagc
    17521 cactgctggt atggctgctg gccgcagatg ttttccttga gctcgtcgtt gcggagcacg
    17581 agccccttcg cggtgtcgac caggatcatc tggcctggtc cgagccgccc cttctccacg
    17641 acggcggcct gatcgatctc gaccgtgccc gcctcggagc cggcgacgaa gaggccgtcg
    17701 cgcgtgattg cgtagcggag tggccggagc ccattgcggt cgagcgtcgc accggccagc
    17761 acgccgtccg agaagccgat ggcggcaggg ccatcccact gctcgatcag tcccgcgtgg
    17821 aagtcgtaga aggcgcgacg ggccggaccg atctccggca gttgctccca cggctcgggg
    17881 accatcatca tcagtgagcg cggcaaggtg cggccgccat ggtgcaagag ttccagcgcg
    17941 ttgtcgaaac tcagcgaatc gctgccgtgc tgcgataccg ccggcgtgat cagctcggta
    18001 tccacgccct ccatccggag gatcggctcc cgtgctgtca tccacttgcg gttgccctgg
    18061 atggtgttga tctcgccgtt gtgcgcgatg tagcggaacg gctgagccat gccccaagtc
    18121 gggaaagtgt tggtgctgta gcgctggtga aagagcacga tcgcgctctc gacccgctca
    18181 tcctgcaggt cgaggtagaa gcgagcgagg tcttccggca ggaagaagcc cttgtagatg
    18241 agggtccgcg ccgacatcga ggcgatgaag aagtccggca tcgtcgcggc ggcggcctcg
    18301 cgctcggcga tacgccgcgc aaccatcagc tccttctcga acgagtcgac atcgagtctc
    18361 tccgggcggg cgatgaatgc ctgccagatg tccgggagcg tctttgccgc ggtatcaccg
    18421 aggacgctcg ggtcgaccgg cacctcgcgc cagccgagca gcggcagacc acgccggccc
    18481 agggcttgct cgatgatctg cttgccaccg gcgacgccgt ctcctggcag gaacgccatc
    18541 gcgatcccgt actcacccgg cgcgaagtcg ctcgcgccga cgccctgcag cacctcggcg
    18601 aagaaccggt gcgggatctg cagcgagatg ccggcgccgt ctccggtgcg ggcatcggct
    18661 ccgacaccgc cacggtgcgt caagttgacc agcccggcga gcgccttctc gacgatctcg
    18721 cgggactgtc ggccgtggat gtccaccacc aggccaacac cgcagctgtc atgctcgaat
    18781 cgggggtcgt acatcggggc atactgtcgc atcgtcgctt ccatcgtgcg ggctcctcct
    18841 gccacgggtg acgtgataag cagctttgac aacagagatg ccggactacc cggcccacgg
    18901 ccggatcatc cggtcagatt cagcgcacaa atgtgcgagc ggttgcgctg gagtagtgca
    18961 tcggtgcagc tggcgcagtc cggaacatga cgcctcagga cactcgatcc cgacctggac
    19021 cgcttcgcac cacgtcgttg tggggacttt cagtgtaggg agatgggcat ccaggcgtac
    19081 aggtactgag cgccaagaat caacgcgccg atatttggac gttccgccca attgcagtgt
    19141 gtaatgcggt atcctccctg caagacgggc acccgtccgt gcgcatcacg tcgatttcgg
    19201 ctgatatacg tggacctcgc agtgaccgga caccggctgc tggataccta cttggaccga
    19261 gtgcacagat tgttcgaatc gtcacattca atacagccat cggctgccgc gatcgacggc
    19321 cccggtgagg agaaccatcc ctatggcacg tgtcgccgga cacctggccg cctcgcagct
    19381 cgaccgcgac ccggaagcga tccacgcggc aatcgaccgc atcgccgagc atgaggttcg
    19441 gctgatcgat ctccaattca gcgacattgc tggcggcatc aaggcgctga cgatcccggt
    19501 caacctcgtg gagtcgacct tccggcgtgg ctaccgcttc gacggcgctg cgctcagtgg
    19561 tgggcggagg caggtcgagc tcgatctctt tctcgcgccg gaccccggca cgctgacgat
    19621 cctgccgcca tacgaccgcg agcctcgacg cgcccagctc ttctgctggg tactgcgccg
    19681 ggacggccag ccgttcgccg gcgatcctcg ctccgccctg cagcggcagc tcgagcacgc
    19741 cgccgaccgc ggattcgact acaccgccgg catcgaaatg gagttctacc tgctttcgaa
    19801 ctccgatctc gccagcacct cgccccttcc tgcagcagat gccgccggct acttcgacgt
    19861 cggcgaagac gtgctcacca gcacccgcaa cgagatcgtc gacacgctcg caacgatggg
    19921 catcggcgtc ggcggcgccc atcacgagac cggcccgggc cagcaggaac tggatctctt
    19981 ccccaccggc ggcatcgcca tcgccgatca gatcgtcacc acacggcagg tgatccgcac
    20041 gatggccaga cgccgcaatc tgcgggcgac cttcatgccg aagcccttcc cggatgcgcc
    20101 tggctccggc atgcatgtct tccagcagat ggcatccctc gacagcggcg ccgacctgct
    20161 gcgggacggc gcggcagccg acgacatctc ggacctggct cgacgagtga tcgccgggct
    20221 gcttggccac gcgccaggga tgtcgatcat cgccaacacc accgtcaact cctacaagcg
    20281 gttggaagcc ggccaccgcg cgccccgaca cgcctcgtgg gcccgcgtca gtcaggcgtc
    20341 gctgatccgg gtaccttcag cgctggaggg cgcacaggcc gacatcgagc tgcgcagccc
    20401 cgacgcgatg accaacccct acctgctctt tgcatccgct ctgggcgccg cactggcagg
    20461 gatcgacgag ggtgaggagc cgacatcacc tctcgacgag ctactcgtgc agtacgacga
    20521 cgacgagttc aatcggcttg gggtcccccg cctgccaacc acgttcggcg aggcactgga
    20581 tacattcgct gccgacgatg tcatccggga atcagtggga agctacatcg ccgaccagct
    20641 gctgcacgtc aagaaggccg agtggctcgc ctacaagtcg cacgtcagcc cttgggagca
    20701 tctgcgctat ggcgacctgt gatgcgcgaa ccccatcagc ccttgtacgg gcagaccttg
    20761 tgtccgcccg tacgggtcga ttccatgtcc gccccggtag acggcgtgcg cacgcgcctt
    20821 acaccagccc ggcggatatc ctgcggaacc cgcccctacg aatcggtatt ggctcgtccg
    20881 cgctggagac cctccgatga tcaccgtgca ggatgtcctc cagcaagcac tgccggcgac
    20941 gacccgcgtg atcgccggca cagaccacct cggccgtgag gtcacgtggg cgacccgcct
    21001 ccggtctgcg ccacccgggt tcgagcacct ctcgggcggc gaggtcgtgc tgctgccgcc
    21061 gcgtgtcctg gaagagctgg acgagcgcct cacccttgcc gacgccatcg agcagcttgc
    21121 gacattccag gtcgccgccg tggcagtcac ctcgaatccc ggcgcccgtg cgcgggccgt
    21181 cgccaacgcc gccggtatcc ccctgctggt gctgccacgc tcgaccgatc tcagttcgct
    21241 ggaacgacag acggcccgct tcatcaccga ccagcgccgc gcgatccagc accgggggca
    21301 ggaagtcacc cgcaagttga tggagctggc gatcgctggc gagccgctgg atgagcaggt
    21361 cgcaacgctc gccgacctgg cccatcgcac agtggtggtc gagagccgtg atggccggtt
    21421 gcttgcctcc cacacgccgc acggcgaccc ggtctcaccg gaagtgcacc agttggtccg
    21481 ccagacgcgc gacagcatcg gtccgtggct ccggtcagtg gcgactacca gcgccgctga
    21541 gccggccacg gcaatctttg aaggggccaa cggctggcgg cgggtggtcg cgccagtggt
    21601 cggacgtgac ggtctgcttg gcagtctctc gctgatcgtc gacaacgaga ccggcacggc
    21661 cgccgaccag atcctcaccg cccgcggcgc agccgcctgc gccgtcacgc tcgcgcggga
    21721 tcaggccgct gcctacgccc ggcgtgaggt agagctcaac gtcctcgacg agattctcga
    21781 cggtgcgctg cgcagcgagg tctccctcgc acagcagctc aagcgcctcg ggcacgacat
    21841 cgagcagcca cacgtcgccc tgatcgtccg tgcccaaggt gaggatggcg tcagccgcgc
    21901 gcgggccggt cgatggtcga tgctcgagga gggaatatcc cgggcagcga gccgcttcgg
    21961 cacgaagagc ctctggcgtg cgcggggatc aagcgcggag atcgtctggc cggtgtcaga
    22021 ccccacagag gcatcgcaag tcgcttcggc gttgcacgcc gaaatccgtg atgcccttgc
    22081 ccgccacggc gtccaagaga ctgtgtcggt cggcgttggg agggtcggca gcggcttcga
    22141 tgccgtaagg cagtcacacc aggaggcacg acaggcgctg atgctcggac gtcgggttcg
    22201 aggatcgggc caggtgacgc tgttcgacga cttgggcgta tatcggctga tctacgcggc
    22261 ggaacacctg ccggagctgc aggggtttat ggcagagtcg ctggggccgc tcatggccta
    22321 tgaccaacag cacggggccg atctggtcag gacgttggaa gccttcttcg aggcgaactg
    22381 cagtcccaag gagacggcgg cgctgctgca cgtccaccgc aacacggtgc tctaccgcct
    22441 cgaccgcatc gccagcatcc tgcaggccga cctcaacgat ccgacgatac gcctgcgttt
    22501 gcaccttgca ctgcacgttc gactcgcgct gacggcgtag cgcccgatga ggcggatggt
    22561 gggtgttcca gatcaagaca ggttgtcatt tcgaccaagg gccggcagac ccgcgtggag
    22621 aaatctcttg cgcgattacg gtcaagacac gatcgcttgc tccatcggag cacgggattt
    22681 ctccactgca cgcgctgggc gcgttccggt cgaaatgaca cgctgactgc tagcaggttc
    22741 tgcgcccggc cctacacccc cactgccgtc gtggcggcag cgatgcggtt gagcgcctcc
    22801 tggatgttcg cctcggaagt cgcgtaggag agccgcaggt acccctcacc ggccgcgccg
    22861 aagctggttc ccgccagcgc ggcgacgccg tgctcgttca gcaacaggtc agcgaactcc
    22921 cggctcgact tgccggtgcc tgtgatgttc ggaaacgcgt aaaatgcgcc cttcggcaac
    22981 acacacgaga tgccgtcgat cgcattcagg ccatcgacga taaggtctcg acgccgacgg
    23041 aacgcctccc gaaactcggc gaccttgtcc tgcggtccgg tcagcgcggc cagccccgct
    23101 cgctgcaccg ccatcgacgt gcaggagacg ctgttgacca tcagcttggt gatcggttcg
    23161 accagctccc gcgggaagag cccgtagccc agccgccagc cggtcatcgc ataggtcttg
    23221 gaaaacccgt ccagcacaat cgtgcgctcg gccatgccag gcatcgtcgc gatggagaca
    23281 tgctcgccct cgtagagcag ctcgctgtag atctcgtcgg agagcacgac aacgtcgtgc
    23341 cgcaccgcca gctcggcgat cgcctcgagg tccgcgcgtt ccagcacacc gccggtgggg
    23401 ttggccgggc tgttgatgat catgaacgtc gttcggtcgc tcaccaacga agtgagctca
    23461 tcgatgtcca gtcgaaagtc gttctcctct cgaaggggga tcggcaccgc cttcgcacca
    23521 ctgtagtcaa tcatcgatcc gtagatcgga aagccgggat cgggatagat cgcctcgtct
    23581 ccctgctcca gcagcgcgag gatcgcgaag aacatcacgg gcttgccgcc cggcgtaacg
    23641 accacctgat cgggcgagaa cgacgtgccg cgcgagcggt tgatgtagtc ggcgatcgcc
    23701 tccttgaggt ccggctgccc ggcggcaggg ccatagtgcg tccatccctc gtgcagggcc
    23761 tgcgcaccgg cctcgatgat gtgctccggg gtgtcaaaat cgggctcgcc gagctcgaga
    23821 tgcaccacat cacgtccctg cgcctcaagc gcgcgggctc gcatcaggac ctcaaaggcc
    23881 gtctccgtcc cgagccgctg catccggctc gccagttcga tcgacatcgt tcgctccttc
    23941 tgcctgcgct acagttctgc acgcccacca ccatcggcca tgatcggcat cagtttcgca
    24001 tgctgccggg ctagctgtcg ccactccgcg cttgacccgg acgcagcctt gacgttcctc
    24061 gccaccaccg gatactgcat tgcatacagt gtgcgatgat cagccaaccc cgcttccggc
    24121 gatatcggat ggcggccacc agtgaaccgg tatcacgagt gccggttcga cgaccgaccg
    24181 tccctggcga gagaaatgcc gatgaccgat gccagtgatg cctccacacc gccccgaccg
    24241 ctgatccagc gccgcaacct gggcgacgac gtctaccaca tcctctggtc acgaatcgtc
    24301 tcgcgcgacc ttcacccagg ggagaaactc tccgatgtca agctgagcaa cgaattggga
    24361 gtctcccgca cacccgttcg cgaggcactg caacgactcc tgcgggatgg cgtgatcgag
    24421 acccggccgc accgcggctt cttcgtcaga tccttcagcg ggaaggacat cgaggagatt
    24481 ttcgagattc gggcggcgct ggaggccctg gcgctgcgga cgctggtgct gcgaagccct
    24541 gacgaggact atggatgggc gttgaagcaa ctcgacgagg tggccgagat cattgccagt
    24601 gcgggcactg aggaggagat cgtctgtgcc aacgaacggt ttctcgagat cgaccagggt
    24661 ttccacatgt tcctggtcga tcgctccgga aacgagcgcc tgaaaaacat catcaacggc
    24721 atctgggcgc agatcgcggt gttccagaaa gcgggcaccc acgtgccgag gtacatcgaa
    24781 attgcgcttc gccaacaccg ggagatcatc tcgctgctgc ggaagaagcg ccacgagtcc
    24841 gcgatcgagg cgctggagaa tcacaatcgc gtcatgaagg agcggttcat cgccgatctg
    24901 gccgaggacg cccgggaggc cgaagccaca tgagaccgat tgcctgggag taccacctcg
    24961 agccagccgc cgttctcgat gccgatcggc tcaatgcgct cggccgggag gcctgggagc
    25021 tggcgacgct cgtaccgagc accgaacagg caatcttcaa gcgccccgcg cttgactatc
    25081 gcgagcggat cacgctggag cagcgagcgc aggtcgagca ggggcggcag gagggcgagc
    25141 gatgaagcga gggggcatcc ttcacccgga gatttcgcgc ctgctggcca gcaccggtca
    25201 caccgactac ttcctcgtca gcgaccgcgg cttcccggtg ccgcccgagc ccgagcgcat
    25261 cgatctcgcg ctggtcgacg acatcccgac ggtgatggat gtgctgcgtg cggtccacgc
    25321 cgagttcgtg atcgaccgga tcgtcatcgc agaggagatg gagcagttct cgcccggccg
    25381 agtcgccgag ctgcgtcacc tgatgggagc cgtgaccatc gagatcgtca gccaaatcga
    25441 gctcaagcgc ctcggccatg acgccaaggc aaccatccgc accggcgaca ccacccccta
    25501 cgccaacctc atcgtcgtct ccggctaacc ctgcttcaac acctcgatga tctggcgaaa
    25561 gaggcgaagg tcctccatcg agtcctccgg tgacgtcttc ggcgttgtct tgccggttac
    25621 cacatcgtga aagacctcca gctcgtgcgt gtagggatcc ttgaaagttg gtcgccgaac
    25681 ggtttcgacg taggcgtccc cgatggtctc cccgatgacc agcgtcgtcg gcaaatgccg
    25741 gatatagggt gtgtcgtact ggacgcgaat ctggcgcgt
//
LOCUS       DASYWL010000232         8746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_311217, whole genome shotgun sequence.
ACCESSION   DASYWL010000232 DASYWL010000000
VERSION     DASYWL010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8746
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311217"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            415..3087
                     /locus_tag="VGR22_08560"
     CDS             415..3087
                     /locus_tag="VGR22_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HEV2128654.1"
                     /translation="MDPAADEGLMGNDGFSDTGSQPGEIVDLDAPLSRVVARGSRRST
                     LAAAEGAFVYDEEHDEGLDYRRRYRGLIGVASKVPIRDRSILSLVYTPGVAEACLAID
                     EDPRRSFDLTCRGNTVAILTDGSDLFGREKGPVEAEIPVAEGKSVIFKTFAGVDAFPI
                     SLATNDASEIVETGIAISPTFGAICLDDISSPRAFTIADNLDKAIDIPVFSNQHHGTA
                     ILVLGALFNALKVVDKKLSDVRVVMSGAGIAGIGVARLLTRAGVRNLVVCDRAGAIYQ
                     YRPERMNWAKAYVAKETNPDQRKGSLREMMAGADVFIGLSIRDIVDEEMVRSMADGPI
                     VFALAVPQPEVLPEVARAGGARVVATGRSDFPNTMDVSLVFPGVFRGLLDSGARSIRL
                     RMMIYAAQALAGMVGEDELHADYIVPRIFDFRVGPAVAAAVVRGAIEAGEATREISPE
                     EVYENTRRFVYEGRLDPARPSVRAASTSFREEAIDLRKRHRGILEIRSKIPIRDHHIL
                     NLLYVPPHALVPAHVIRDDSSKVDELTARGNLVAIVTDGSAVLGLGDIGPQAALPVME
                     GKAVLFQTLAGVEAFPVTLASRDVDEIVKIVKSFAPSFGGINLEDISAPRCFEIEERL
                     RAELDMPIFHDDQHGTAIVVAAGLINAAKIRKTDISEMRIVINGAGSAGIAVAKLLIA
                     LGADVTVCDRKGAIYHDRDEQMDVSKRRIAEVTNRQGCTGNLRDAVEGADVFIGLSAP
                     DTLTKEMITSMAKDPIIFALANPTPEIMPDEAHAAGALAVATGRSDYPNQVNNSLAFP
                     GVFRGALDVKARTISEEMKLAAAHAIADLVAPSQLSPEFLIPDSLDLRVAGRVAAAVA
                     RAAREQGLSRKDTSPEQIEAHTHDLVYEGTIALA"
     gene            3106..4254
                     /locus_tag="VGR22_08565"
     CDS             3106..4254
                     /locus_tag="VGR22_08565"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2128655.1"
                     /translation="MANEIHTIPTERGIVRVALMRDNEELSRTLVVPMTMRIGRSVLR
                     MDGIGGVATPEQHRNKGYSRRVLEAAVDYMLAGDAGLSTLYGIPHYYPKFGFATIGPE
                     YTLWLRSLDTRDTLPEGITEREGRPGDLSALQWLYHEETSKAYGAVVRDDDWWVWERL
                     ETALEPGTGEVRVVEHHGEVVGYAWRGSDFWWMQHLHRDTEPALKVAEAFAADHVAAD
                     AVLAMCRRWARSLEQERCDLAMPPESRVARAAMLQDASLTVRHHDEAEFMGRVTGLVA
                     LLRSLAPELEARWFLCGATSPRFAVTIVCGAERATINGSETGVIVEPGGGGDMTAHLD
                     PGTMARLAMGGFPPETVLERAGVAASVRPILDVLFPHVVPYIYPADRF"
     gene            4285..4752
                     /locus_tag="VGR22_08570"
     CDS             4285..4752
                     /locus_tag="VGR22_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128656.1"
                     /translation="MSEQSPSSTRDRVVERFEWHPEHAGRTVEDVREELEREIAADQR
                     QYRIAMEGAEEAEQDALASVVTLERRWGRYDFGWAELDAGDIASRIVEFEQERERRRE
                     MISWSEYWNVHGSGGMTDSGESSSAELSTGMKAISLGLVVLLIVLILLAVWAL"
     gene            5036..5671
                     /locus_tag="VGR22_08575"
     CDS             5036..5671
                     /locus_tag="VGR22_08575"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEV2128657.1"
                     /translation="MALHNVSTIPQTEPPVRPAWLAMSHPPVLNMRGEQIGLGPLSAD
                     LLAVYQRWLNDFDLLVMLDRRFRPLTNDWIRTWFERQSRGNGDTLVFTIWDLATMTPI
                     GNTALQDVDVRTRTAEFGIFIAEPQFRGDGRGTETTRMMVRFALETLALENVMLRVFD
                     DNEPAIRTYEKVGFKEIGRRRGAHRRNGIPGDIVLMDITPADLRPVMTAAD"
     gene            complement(5821..6984)
                     /locus_tag="VGR22_08580"
     CDS             complement(5821..6984)
                     /locus_tag="VGR22_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV2128658.1"
                     /translation="MITADYDSLTAEDLRGVLGVKWTMFPDCIGAFVAEMDFATAPPV
                     IAALHRAVNAGFFGYLPKEKAEALSAATAAWYRANTGWEVLPERIHPLPDVIKGLEAT
                     LEHYAPKGGKVIVPTPAYMPFLKVPIIHNREMIEVPMREVDGHYVYDLDALDAAFADG
                     GEVLIVCNPHNPISRVLERDELVAISEVVARHGGRVFSDEIHAPLIYGGREHVPYASV
                     SEAAAGHTITAISTSKAWNLAGLKCAQIVLSNEADVETWEAGASWAGHGTSTMGVVAS
                     IAAYTEGQPWLNEVLAYLDGNRRALGELIAEHLPKVSYTMPEGTYLAWLDFSKTPIDG
                     DLGEFFRDNAGVAIVDGSACGEAGNRHARFNFAMPRPVMEQAIRRMGEALRKV"
     gene            complement(7238..8632)
                     /locus_tag="VGR22_08585"
     CDS             complement(7238..8632)
                     /locus_tag="VGR22_08585"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEV2128659.1"
                     /translation="MLDSRPNILYIMTDDHAAHAITAYGSVINETPNIDRIANEGARF
                     DNVFCGNAICAPSRATILAGNHSHINGVLTLADRFDGRQPTFPKAMQAAGYQTAIFGK
                     WHLGHGGEADPTGFDTWTVLPGQGDYHDPTFFVQGEEQRFEGYVTDLITDFSIDWLEQ
                     RDPERPFLLKVHHKAPHRPWDPDEKHEHLYEDIDIPIPETFDDDYATRPAAEAAEMRV
                     GRDMTFRDLKEPPPKGLSPEETRRWAYQRYIKDYLRCVASVDDNVGRLLDWLDDNGLA
                     ENTVVVYTSDQGFFLGDHGWYDKRFMYEESLRMPFLMRYPPEIEANSVVDAIVSNIDF
                     GPTFLDYAGLEAPEMMQGASFREVAKGNVPEEWQESIYYRYWMHLANHGVAAHYGVRT
                     ATHKLIYYYGRALGTSDSVDQDTEQYWELFDLEADPKELNNVYGKPDYTEVQEALHAE
                     LARLRQRYGDTDAV"
BASE COUNT         1430 a         2768 c         3009 g         1539 t
ORIGIN      
        1 ctggtgcgac gccagtgcct gccgatgcgg atgcggcacc ggcgaccccg gtggctgcga
       61 ccccggctcc cgtcggcgcg acgccagtcg ctggtgcgac gccggttccc gctgacgtag
      121 atgcggcacc tgcgaccccg gtcgcgtctc cgatggcgac accggaggcc tagccggtcc
      181 acctgcctta ccggcgatac cgtggtgaca gagaacgagc gacgcgggtg ccgacgaacc
      241 ggcacccgcg ttttgccgtt cggcagcaac caagcggagc ggtcatggcc ggtggcgtaa
      301 cgctggtaga gtagatgcca gcaaggtggc ggcgcgtgtt gccgatggtg accacgggga
      361 cagcgggcag gcgcaggagc attcgtgaac agcaatgggc aggattttac gattatggac
      421 cctgcagcgg acgagggcct gatggggaac gatggatttt cggacaccgg gtcgcagccg
      481 ggggagatcg tcgatcttga tgctccgctg tcgcgggtag tggcgcgtgg cagccggcgc
      541 agcacgctcg ccgcggcgga gggtgccttc gtctatgacg aggagcacga cgaggggctg
      601 gactatcgac gccgctatcg ggggttgatc ggcgttgcca gcaaggtgcc gatccgtgac
      661 cgctccatcc tctcgctggt ctataccccg ggtgttgccg aggcctgcct cgccatcgac
      721 gaagatccgc gtcgcagctt cgatctcacc tgtcgcggca acacggtcgc gatcctgacc
      781 gatggctcgg atctcttcgg gcgcgagaag ggccctgtgg aggcggaaat ccccgttgcc
      841 gaaggcaaga gtgtcatctt caagacgttt gccggggtcg acgcgtttcc gatcagcctc
      901 gcaacgaacg atgccagcga gatcgtcgag accggcatcg cgatcagccc gaccttcggg
      961 gcgatctgcc tcgacgatat ctcctcgcca cgagcattca cgattgccga caacctcgac
     1021 aaggcgatcg acatcccggt cttctccaac cagcaccacg gcaccgccat cctcgtgctc
     1081 ggggcactct tcaacgcgct caaggtggtc gacaagaagc tcagcgacgt ccgcgtcgtg
     1141 atgagtggcg ccggcatcgc cgggatcggg gtcgcccgcc tgctgacacg cgccggtgtc
     1201 cgcaacctgg tcgtctgcga ccgtgccggg gcgatctacc agtaccgccc ggagcggatg
     1261 aattgggcca aggcctacgt tgcgaaggag acgaatccgg accagcgcaa gggttcgctg
     1321 cgcgagatga tggccggcgc agatgtcttc atcggtctct cgatccgcga tatcgtcgac
     1381 gaggagatgg ttcgaagcat ggcggacggc ccgatcgtct tcgcgctggc ggtcccgcag
     1441 ccggaggtcc tgccagaggt tgcgcgggcc ggtggcgcgc gagtcgtcgc gacgggccgc
     1501 tccgacttcc cgaatacgat ggacgtctcg ctcgtgttcc cgggcgtgtt ccgcggtttg
     1561 ctggattccg gcgcgcggag catccgcctg cggatgatga tctacgccgc ccaggcgctg
     1621 gccggcatgg tcggcgagga cgagctgcac gccgactaca tcgttcctcg gatcttcgat
     1681 ttccgggtgg gaccagcggt ggccgcggcg gtggtgcgcg gcgccatcga ggccggcgag
     1741 gcaacgcgcg agatttcgcc ggaggaggtc tacgagaaca cccgccgctt cgtctacgag
     1801 gggcggctcg acccggcccg tcccagcgtg cgtgcagcga gcacctcctt tagggaggag
     1861 gcgatcgacc tgcgcaagcg gcatcggggc atcctggaga ttcgcagcaa aatccctatc
     1921 cgcgaccatc acatcctgaa cctgctctac gtgccgccgc acgcgctggt gccggcgcac
     1981 gtcattcgcg atgattcctc gaaggtggac gagttgacgg ctcgcggcaa cctcgtggcg
     2041 atcgtcacgg acggttccgc tgtgctcgga ctgggtgaca tcgggccgca ggccgcactg
     2101 ccggtgatgg agggcaaggc cgtactcttc cagaccctcg ccggcgtcga ggccttcccg
     2161 gtcacactcg cttcacgcga cgttgacgag atcgtgaaga tcgtcaagtc gttcgcgccc
     2221 tccttcggcg gcatcaatct ggaggacatc agcgcgccgc gctgcttcga gatcgaggag
     2281 cggcttcgcg ccgagctcga catgccgatc ttccacgacg accagcatgg cacggcgatc
     2341 gtggtggcgg cagggctcat caatgccgcg aagatccgca agaccgacat cagcgagatg
     2401 cgtatcgtga tcaacggcgc ggggtcggcc ggcatcgccg tcgccaagct gctgatcgcg
     2461 ctcggagccg acgtcaccgt ctgcgaccgc aagggcgcta tctaccatga tcgcgacgag
     2521 cagatggatg tctccaagcg tcgcattgcc gaggtcacga accgccaagg ctgcaccggc
     2581 aatctccggg atgcggtcga gggtgccgat gtcttcatcg gactgagcgc gccggacacg
     2641 ctcaccaaag agatgatcac gagcatggcc aaagatccga tcatcttcgc cctggcgaac
     2701 ccgacgccgg agatcatgcc cgacgaggcg cacgccgccg gcgcgctggc cgtcgccacc
     2761 ggccgcagcg actacccgaa tcaggtcaac aactcgctgg cgtttccggg cgtgttccgg
     2821 ggagcgctgg atgtcaaggc acgcaccatc agcgaggaga tgaagctggc agcggcgcat
     2881 gcgatcgccg acctcgttgc gccgagccag ttgtcgccgg agttccttat cccggacagc
     2941 ctcgacctgc gcgtagcggg ccgcgtggcg gctgccgtgg cgcgcgccgc tcgcgagcaa
     3001 ggactctccc ggaaggacac cagcccggag cagatcgagg cgcacacgca cgatctcgtc
     3061 tacgagggca ccatcgcgct cgcctgatcg cggcgggggt acgccatggc caacgagatc
     3121 cacaccatcc cgaccgaacg cggcatcgtc agggtcgcgc tcatgcgaga caacgaggag
     3181 ctgagccgga cgctggtggt gccgatgacg atgcggatcg gacgctcggt gctgcggatg
     3241 gacggcatcg gtggcgttgc gacgcccgaa cagcaccgca acaaggggta ctcccgtcga
     3301 gtgctggagg cggccgtcga ctacatgcta gctggcgacg cagggctctc gacgctctac
     3361 ggcatcccgc actattaccc gaagtttggt ttcgccacca tcggaccgga gtacactctc
     3421 tggctgcgat cgctggacac gcgggacacg ctgccggagg ggatcaccga gcgggagggt
     3481 cggccgggcg atctctccgc cctgcagtgg ctctatcatg aggagacctc gaaagcgtac
     3541 ggcgcggtgg tccgcgacga tgactggtgg gtgtgggagc ggctcgagac cgcgctggag
     3601 cccggtacgg gtgaggtgcg agttgttgag catcatggtg aggtggtcgg ctatgcctgg
     3661 cgcggcagcg acttctggtg gatgcagcat ctccatcgcg atacggagcc ggcgctgaag
     3721 gtcgccgagg ccttcgcggc ggaccacgtc gccgccgatg ccgtcttggc gatgtgtcgt
     3781 cgctgggcgc ggtcgctcga gcaggagcga tgcgatctgg cgatgccgcc ggagagccgg
     3841 gtggcacggg cggcgatgct ccaggatgcc agcctcacgg tcaggcacca cgacgaagcc
     3901 gagttcatgg ggcgggtcac aggtctggtg gcgttgttgc ggagtctcgc accggagctc
     3961 gaagcccgtt ggttcctgtg cggcgcaacc tctccccgct tcgcggtcac gatcgtctgc
     4021 ggtgcagagc gggcaaccat caacggaagc gagaccggcg tcattgtaga acccggcggg
     4081 ggtggcgata tgactgctca cctcgatccc ggcacgatgg cgcgtctggc gatgggcggg
     4141 tttccgcccg agacggtgct ggagcgagcc ggggtggcgg cgtctgtccg gccgatactg
     4201 gacgtgctct ttccacacgt tgtgccgtac atctacccgg ccgaccggtt ctgacagctg
     4261 cccgggccag cgacaggaga cttcatgagt gagcaaagtc cgtcgagcac acgcgaccgc
     4321 gtcgttgagc gctttgagtg gcacccggaa catgccgggc gcactgtcga ggacgtgcgc
     4381 gaggaactgg agcgggagat cgcggccgac cagcgacagt acaggatcgc gatggagggc
     4441 gccgaggagg cagagcagga cgcgctcgcc tcggtggtca cgcttgagcg acggtggggc
     4501 cgctacgact tcgggtgggc ggagctcgac gctggcgaca ttgcgtcgcg gatcgtcgaa
     4561 ttcgagcagg agcgcgagcg ccgccgggag atgatcagct ggtcggagta ctggaatgtc
     4621 cacggctctg gcggcatgac cgacagcggt gagtcgtcgt ctgccgagct gagcaccggc
     4681 atgaaggcaa tctcgctcgg gctggtcgtg ctgctgatcg tgctcatcct cctcgctgtg
     4741 tgggcgctgt agcgtttccc gggcgccttc cgcgttccca ccagccttgg gccttcctgc
     4801 gtgagccatc cggatcgcgc actgttagta aactatcgct cgcggtgcaa tcgatcgttc
     4861 cagggcaagc cctccagaga aaaagcgtcc gtacacctgg cgcctgttcc tcaccgccgc
     4921 catactcctt gtaaggttcg ttcgtacaca gttatcgaat gtgccgcgac cgctgcgtta
     4981 cgcggcaatg tgacgttccg acgcgaggag ccagaaaggt accgaggaga gccgcatggc
     5041 cttgcacaac gtctcgacca ttccgcagac cgagccgccg gtcaggcccg catggctggc
     5101 catgtcgcac cctccagtgc tgaacatgcg gggagagcag atcgggctcg ggccgctcag
     5161 cgccgacctg ttggcggtct accagcgctg gttgaacgat ttcgatttgc tggtgatgct
     5221 tgaccgccgc tttcggccgc tgaccaacga ttggattcgg acgtggttcg agcggcagtc
     5281 gcgcggcaac ggcgacacgc tggtcttcac catctgggac ctcgcgacca tgacgccgat
     5341 cggcaatacc gcgttgcagg acgtcgacgt gcgcacgcgc acggccgagt ttggcatctt
     5401 catcgccgag ccgcagttcc gcggggacgg tcgggggacg gagacgaccc ggatgatggt
     5461 ccggttcgcg ttggagacgc tggcgctgga gaacgtgatg ctgcgggtct tcgacgacaa
     5521 cgagcccgcg attcggacct acgagaaggt cgggttcaag gagatcggtc gtcgccgagg
     5581 cgctcaccgg cgcaacggga tacccggcga catcgtgctg atggacatca ccccggccga
     5641 tcttcggccg gtgatgaccg ctgccgactg atgcagtcac tcttgcagac gtgtgccgcg
     5701 aatagaggcg ggcaccgcgg gtgctcgcct cttcttgcgc gttcgcggtc ggctacctcg
     5761 aacgaagtga cgccggagtg accgggccca ccggtcgggt cgcgaggctg cctgctgcag
     5821 tcagaccttc cgcagcgcct cgcccatcct gcggatcgcc tgctccatga cgggccgggg
     5881 catcgcgaag ttgaaccgcg catgccggtt gccagcctcg ccgcaggcgc tcccgtcgac
     5941 gatggcgacg cccgcgttgt cccgaaagaa ctcgccgagg tcgccgtcga tcggcgtctt
     6001 gctgaagtcg agccacgcga ggtacgtccc ctccggcatg gtgtagctca ccttcggcag
     6061 gtgctcggcg atcagttcgc ccagggcacg gcggttgccg tcgaggtagg ccagcacctc
     6121 gttgagccat ggctggccct cggtgtaggc cgcaatgctg gccaccacac ccatcgtcga
     6181 ggtgccgtga ccggcccagc tggcgcctgc ctcccacgtc tccacgtccg cctcgttcga
     6241 cagcacgatc tgcgcgcact tgagcccggc gaggttccac gccttggacg tggagatcgc
     6301 cgtgatggtg tgaccggcgg ccgcctcgga gacggatgcg tacggaacgt gctcgcggcc
     6361 cccgtagatc agcggcgcat ggatctcgtc cgagaagacg cgcccgccgt ggcgcgcaac
     6421 cacctcgctg atcgcgacca gctcatctcg ctccagcacg cggctgatcg ggttgtgcgg
     6481 attgcagacg atgagcacct cgcctccgtc ggcgaaggcg gcgtcgaggg cgtcgagatc
     6541 atagacgtag tggccgtcaa cctcccgcat cggtacctcg atcatctccc gattgtggat
     6601 gatggggacc ttcaggaagg gcatataggc gggggttgga acgatcacct tgccaccctt
     6661 cggcgcgtag tgctccaggg tggcctcgag ccccttgatc acatcgggga gcgggtggat
     6721 ccgctctggc agcacctccc agccggtatt ggcccggtac cacgccgcgg tcgccgccga
     6781 gagcgcctct gccttctctt tggggaggta cccgaagaac cccgcattga ccgccctgtg
     6841 cagcgccgcg atcaccggcg gcgcggtagc gaagtccatc tccgcgacga aggctccgat
     6901 gcagtcgggg aacatcgtcc acttgacgcc gaggacacca cgaaggtcct cggcggtcag
     6961 gctgtcgtaa tcggcggtga tcatggcggc gtggttcatc ctctcacgta taggcgcccg
     7021 cgtacacgcg ggtagcgatt gaccctcggc aacatcaggc gtgatggtcg ccacgaagcg
     7081 atccggaggc atggtaagca ctccgcgtgc cactgtcgac aggctgctgc caccgggttc
     7141 ggttcaccct gtggactccg cccggaccac cggcaggagt gggccttccc gcgcggtgtg
     7201 accgtggagt ccgcccgtac gcgagcccgt gtgccatcta cacggcgtct gtgtcgccgt
     7261 agcgctggcg gaggcgtgca agctcggcgt ggagcgcctc ctggacctcg gtgtagtccg
     7321 gcttgccgta gacgttgttg agctccttcg ggtccgcctc gaggtcgaac agctcccaat
     7381 actgctccgt gtcctgatca acggagtccg acgtcccgag cgccctgccg tagtagtaga
     7441 tgagcttgtg cgtggcggtg cggacgccgt agtgcgcggc gacgccgtgg ttggcgaggt
     7501 gcatccagta gcgatagtag atcgattcct gccattcctc cgggacgttg cccttcgcca
     7561 cctcgcggaa gctggcgccc tgcatcattt ccggtgcctc gagcccggca tagtcaagga
     7621 aggtgggacc aaagtcgatg ttgctgacga tcgcgtcgac caccgagttg gcctcgatct
     7681 cgggtgggta gcgcatcagg aagggcatcc gcagcgattc ctcgtacatg aagcgcttgt
     7741 cgtaccagcc gtggtcgccg aggaagaagc cctggtcgga ggtgtagacg acgactgtgt
     7801 tctcggccag gccgttgtcg tccagccagt ccagcaagcg gccgacgttg tcatcaaccg
     7861 aggcaacgca gcgcaggtag tccttgatgt agcgctggta ggcccagcgg cgcgtctcct
     7921 cgggagagag acccttgggt ggtggctcct tgagatcgcg gaaggtcatg tcccggccga
     7981 cccgcatctc ggcggcctcg gcggcgggcc gggtggcgta gtcgtcgtcg aacgtctcgg
     8041 gaatggggat gtcgatgtcc tcgtatagat gctcgtgctt ctcgtctgga tcccatggtc
     8101 ggtgaggcgc cttgtggtgg accttgagca ggaagggacg ctccgggtcg cgctgctcca
     8161 gccaatcgat cgagaagtcc gtgatgaggt cggtgacgta gccttcgaac cgctgctcct
     8221 cgccctgcac gaagaaggtc gggtcgtggt agtcgccctg gccggggagg acggtccagg
     8281 tgtcgaagcc ggtcgggtcc gcctcgccgc cgtgtccgag gtgccatttg ccgaagatcg
     8341 ccgtctggta tcccgcagcc tgcatcgcct ttgggaaggt cggctggcgg ccgtcgaagc
     8401 gatcggcgag cgtcaggacg ccgttgatgt ggctgtggtt gccggcgagg atcgttgccc
     8461 ggctgggcgc gcagatcgca ttgccgcaga agacgttgtc gaagcgcgcg ccctcgttgg
     8521 cgatgcggtc gatgttcggc gtctcgttga tgacgctgcc gtacgcggtg atcgcgtgtg
     8581 cggcgtggtc atcggtcatg atgtacagga tattgggacg gctgtccagc atagattccc
     8641 ttcaaggagc ggtccagagc gggatgtctc tgtgccattg tgctgggaaa taccggagct
     8701 gccagccttg cgcactatga gagcctgacc gggcccggct ccatac
//
LOCUS       DASYWL010000233        10878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_86169, whole genome shotgun sequence.
ACCESSION   DASYWL010000233 DASYWL010000000
VERSION     DASYWL010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10878
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86169"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1036
                     /locus_tag="VGR22_08590"
     CDS             <1..1036
                     /locus_tag="VGR22_08590"
                     /inference="COORDINATES: protein motif:HMM:NF014804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEV2128660.1"
                     /translation="LCIAAGEPLDLAQVEPKGHAIEARVYAEEPGAGFRPAPGRLATV
                     AWPEGPGIRVDAGHASGDEVPHAYDAMLAKVIAHGPNRDTAIERLREALLDTIVAGVS
                     TNLAWLIDLLDSDAFRAEAMTTQTAAEVEPTDPGWGIAPVAALAHMLDEIRATTPDAW
                     TLLGPWRFSGPVPVTLHGADWEARLDVRRVGDHWEIRDVDTGAVARWERGNDDVLSVE
                     MDGEVTRAAVVPKGGGLLEVFARGGRWPVVPGPRSQVTRELQGVASNGQVVAPMPGSV
                     VSVHVAAGDRVDRGAALLTLTAMKMEIVLEAPDAAVVAEVSCAPGDLVAADQVLVTLR
                     PEDRSGSPAT"
     gene            complement(1584..2108)
                     /locus_tag="VGR22_08595"
     CDS             complement(1584..2108)
                     /locus_tag="VGR22_08595"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV2128661.1"
                     /translation="MIQLGVGDGTTLPGAVETDRQRTRVAKLIDKVEAAWADFHAAYT
                     GLNDEEMLIPGVTGEWSVRDLIAHVTWWDEEAITHLPIVLEGGTPPRYSVTYGGIDAF
                     NVRKTAEKRGVSLDDVRREAEETHARLIEYLRSVPPERLKGNSRFTHRLRLDTYEHYP
                     IHTADIHAWRERLA"
     gene            complement(2105..2578)
                     /locus_tag="VGR22_08600"
     CDS             complement(2105..2578)
                     /locus_tag="VGR22_08600"
                     /inference="COORDINATES: protein motif:HMM:NF019632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEV2128662.1"
                     /translation="MQTTDTNLATIIDRIDANWADFRASYAGLSDEEMLIHDVSGDWS
                     VRDILAHGADWDALVLSILPGILETGRHPEHDEANADLDAYNARMTEETRHLSLDEVR
                     RELEDTHRRLLDYLGTVDADGFAANAAFRKRLAADTWDHYPEHTAAIRAWRERRS"
     gene            complement(2771..3385)
                     /locus_tag="VGR22_08605"
     CDS             complement(2771..3385)
                     /locus_tag="VGR22_08605"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEV2128663.1"
                     /translation="MTEIGTAMCGVVTGEDGLARCPWATSHPLNLHYHDTEWGMPVRG
                     EQGLFERIMLEAFQSGLSWLTILRKRDAFREAFAGFDPGIVADFTDADVARLMDNTAI
                     VRNCAKIEAARTNARATVALRDEGGLDTLVWSHQPETTPMPRTPKEVPTVSPESKALA
                     KALKTKGFVFVGPTTAHALMEAIGLIDTHLAGCHRRGCSGLFPG"
     gene            complement(3764..8179)
                     /locus_tag="VGR22_08610"
     CDS             complement(3764..8179)
                     /locus_tag="VGR22_08610"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HEV2128664.1"
                     /translation="MDITRRPDARAGNLAAAPRSISPTDISQFIRLEQCERYLRLRLK
                     ERAEGRDFLYAYDVAPQSIPPILTRSGASFESAVEADIAQQYPTLRFGPERRRELHLT
                     HDNADTMEALATLGAGETVVLFQPRVEATVDGWRLRGDVDLLRAERDAADTLRLLIAD
                     MKSSTASRVEHRLQVAFYHEMLATLLAEAGIAHAPIELAILYRGPVRESLLAPVEDRE
                     DQERQRQDAEQTLGTSAGLLDRVRDVDAYLGSVRDLVTKPESTAHHVLGTDFEEIPFH
                     LTWKCDGCLYNEFCMKRSAETDNLSLLPHITEQDKRALEDNGIATTRQLASLKSLVKP
                     GEMGPDGKVHERTILVPSPGHSDTCERLAATWPVGPRLDELIHRALRYEHWKTKQGTA
                     LSYIPNRGYGSMPHSDAEQNPNLVRIFIDAQHDYLNDRTYMLGALVVANEGGRPHPKR
                     RRSIARLSDGPPDSDEREESLFVDWITETLRTIVELAAPNIEGEAKAPIHLTFVNGFA
                     QRVLLDGLARHKDSILGATALYDFVTQLAAFDSAIASYLETEIREHRNYPMVCQSLQA
                     VAAYLKFDWNAGAPYREIFRTRMFDFWGRFEAPPDGAEEIPGKDGWFTSRARFNSQIP
                     LEYAYAAWGELPVPEEGEDDFATYRGSTPELLQRFHARRLEAMEHIVDRDLPGNRQTA
                     LREFELPDLAAFEEIARDLAHALNEFVVIERHVELAEWKRDRLAEPEQRVLSGQTLIV
                     QYREEDQEPGMAAQNRDNVHRRAMKEEYRARYREAHPDAKQVRLPKEQRAKSDWTNEG
                     LCFRLRITVDDVACDLDEALRLTTIREGDWLVIFPRWTFDSRLPEAERYLFTPTAKQL
                     LYGMRCTLERIVVDRDEQGMAQWAWAEVTMNGTRGGGMAGFTFGTIGDNDRPLTHGEV
                     LTLDPDPSNIYAAWTKQVTEGLIAGGENSLYDILAGREFPEPEWPEAARAAQQRFLDG
                     IDALHAAAAENVYDFELSKREFIGAHGDTPVLLVQGPPGTGKSYSTAYAILARIQGAM
                     AANREYRVFLSCKTHAATDVLLENVAEAQELLRQLRTTHSEIIGRHIDERVLDLPLYR
                     YRSKNDVPAGVTPLPRDKARQDGQAKAVDRLLANRWAVVAATPGGIYALLGDHFGSKD
                     RFGHPLADCLVLDEASQMSMPEAIMAALPLKPGGSVIVVGDHRQMPPIVRHDWASEPR
                     RSFRQFRTFESLFLALLALDPPMIQFAESYRLHADMAKFLRDEIYIHDGIEYHSHKAN
                     LFSDYPTPDDFLAAVLAPEHPLTVVVHDETNSQVRNDFERRLLAPMLQTLSDPARFAL
                     DPHEGLGVVVPHRAQRAALQEDLVRLLGPHSDADEAVVSAVDTIERFQGGERDAILIG
                     VTESDRDYILMNGEFLLDPRRLTVALSRAKQKLVLVAARSVFEVFSADEETFANAQLW
                     KNLLRKTCTVPLWEGERCGHHVAVWGNRSSAATGR"
     gene            8383..8694
                     /locus_tag="VGR22_08615"
     CDS             8383..8694
                     /locus_tag="VGR22_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128665.1"
                     /translation="MMPDDHDQQSETDLTCETSAMEVSGRLSRREILGRSILGITGTG
                     LVSLLAACGGEEEDEALEDVDDQEEDEAADVEEDENPTGIEDLDEAPAGEDPAPGGNG
                     G"
     gene            8985..9284
                     /locus_tag="VGR22_08620"
     CDS             8985..9284
                     /locus_tag="VGR22_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128666.1"
                     /translation="MIQLQRNEHLNIDEEMTLLSRRAMLRRSVLGITGVGLTALLAAC
                     DDDEDDDEDAAGEGIDPEDAMDSIPLDPDSPAIDEETDVVPEEVIPEDAEEEADT"
     gene            complement(9343..10179)
                     /locus_tag="VGR22_08625"
     CDS             complement(9343..10179)
                     /locus_tag="VGR22_08625"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HEV2128667.1"
                     /translation="MGGEPLDTDTRARWQARQRARPEVQPTALAAPRFWEIDTVRGIA
                     IAMMVLYHLVFDLDRFGGFDLNATRGFWRHFATITAFLFVFLVGVSLRVSYDRSRRTT
                     ASEAQLFRKYTLRGVRILGYGMLITLVIGLAVPDGNIYFGILHAIGLSIILAYAFLGR
                     PTLALLVAIPVVLAAPVVRSLHMDEPWLLWLGVRAPVGYMLDYRPMMPWFGVVLLGIV
                     AGSSLYRNGERRFPWPAGQPPWPGRQLATVGRYSLAIYLLHQPLLLAVLSALGLIDLG
                     IF"
BASE COUNT         1767 a         3491 c         3716 g         1904 t
ORIGIN      
        1 gctctgcatc gcggccggcg agccgctcga cctggcgcag gttgagccga aggggcacgc
       61 gatcgaggcg cgtgtgtacg ccgaggagcc cggcgcgggg ttccgccccg cgccgggccg
      121 gctcgcgacg gtcgcctggc ccgaggggcc gggcatccgg gtcgatgccg ggcacgcgag
      181 cggcgacgag gtgccccacg cctacgacgc gatgctcgcc aaggtgatcg cgcatggacc
      241 gaaccgcgac acggcgatcg agcggctgcg cgaggcgctg ctggacacga tcgttgccgg
      301 cgtgtccacc aacctcgcct ggctgatcga cttgctcgac agcgatgcct tccgtgccga
      361 agccatgacg acgcaaaccg ccgccgaggt ggaacccacc gatccgggct ggggcatcgc
      421 gccggtcgcg gcgctggcgc atatgctcga cgagattcgc gcaacgaccc ccgatgcctg
      481 gacgctgctc gggccgtggc ggttctccgg gccggtgccg gtgacgctcc acggcgccga
      541 ctgggaggcg cgcctcgatg tgcgccgcgt gggagatcac tgggagattc gcgatgtcga
      601 caccggcgcg gtggcgcgct gggagcgagg gaacgatgac gtgctctcgg tcgagatgga
      661 cggcgaggtg acgcgggcgg cggttgtgcc caagggcggc gggttgctgg aggtgttcgc
      721 gcggggaggg cggtggccgg tggtgccggg gccgcgctcg caggtcacgc gcgagctcca
      781 gggcgttgcg tcgaacgggc aggtggtcgc gccgatgccg gggagcgtgg tgagcgtcca
      841 cgtcgcggcg ggggatcggg tggatcgcgg ggcggcgctg ctgacgctca cggcgatgaa
      901 gatggagatc gtgctggagg cgccggatgc ggcggtggtc gcggaggtgt cctgcgcgcc
      961 gggcgatctt gtcgccgccg atcaggtgct cgtcaccctg cgacccgagg acaggagtgg
     1021 gtcgccagcc acctagtagc cactctgaag cccgcggttg tatggcatgt cccaatctct
     1081 cggcgactca tgaggccgct gatcaccccg gtatgcgcct tgcggggcct ctttcgatcg
     1141 ccggttgatc tgaaggcaga ccgctcgcag ggaaaaggag tcgatatggc aacatcgcgt
     1201 gatcatgaga tgtatctgga ggtcgacgac ggctaggcag aggctaccga gtaccggagc
     1261 ctcccgttgt cccggcggac ggttctacgc cggtcggcac tgggccttac gggaatcggg
     1321 ctgaccgctt ttctagccgc gtgtggcgac gatgacgagg aagcacccgc cgaggaagaa
     1381 gaggaagagg aagaggacgg gatctaggtt ggacgtcgac ttggccggcg tcgcgattct
     1441 gctcctcggg acaagacccg ggatgggtct gttctggccc gtcccggctg cttggagaac
     1501 ggccgattga gctcttgtcg ctgtctgtcc atggtgggca ctcaagagac gcgctcaatc
     1561 ttggacgggg acaggcagcg ctgtcatgcg agccgttccc gccacgcgtg aatgtcggcg
     1621 gtgtggatcg ggtaatgctc gtaggtatcg aggcggaggc ggtgggtgaa gcggctgttg
     1681 cccttcagtc gctccggcgg gacggaccgc aggtactcga tcagccgcgc gtgcgtctct
     1741 tccgcctcac gacgtacgtc gtcgagcgag acaccgcgct tctccgccgt cttccgcacg
     1801 ttgaaggcat cgatgccgcc gtaggtgacc gagtagcgcg gcggcgtgcc gccctcgagc
     1861 acgatcggca ggtgcgtgat cgcttcctcg tcccaccagg tgacatgggc aatgagatcc
     1921 cgtaccgacc actcgcccgt cacgccgggg atcagcatct cctcgtcgtt cagcccggta
     1981 taggcggcat ggaagtcggc ccacgccgct tcgaccttgt cgatcagctt cgcgacgcgt
     2041 gtccgctgcc ggtcagtctc caccgctccg ggaagggtgg tgccgtcccc cacgcccagc
     2101 tggatcacga ccggcgctca cgccatgcgc ggatcgcggc ggtgtgctcg gggtagtggt
     2161 cccaggtatc ggccgccagg cgtttgcgga aggcggcgtt ggccgcgaac ccatccgcgt
     2221 cgaccgtccc gaggtagtcg agcaggcgcc ggtgggtgtc ctccagctcg cggcggactt
     2281 cgtcgaggga gaggtgacgc gtctcctcag tcatgcgggc gttgtaggcg tcaaggtccg
     2341 cattcgcctc gtcgtgctcc gggtgccgtc ccgtctcgag gatgccgggg aggatgctca
     2401 gtaccagcgc gtcccagtcg gcgccgtgcg cgaggatgtc ccgcaccgac cagtcgccgg
     2461 acacgtcgtg aatcagcatc tcctcatcgc tcaggccggc atacgacgcc cggaagtccg
     2521 cccagttggc atcgatccgg tcgatgatcg tcgcgagatt ggtgtcggta gtctgcatgg
     2581 tagcttctcc tcctggtgtt cgatgccctc ggcaccgtgc gattgatccg gttggctatt
     2641 accagattcg gatgcagcgt ttgggagtcg tgtacgggag gggcttgtcc cctcccacag
     2701 aagggccggg acaagcccct cccgtacgtg aagactgctc aggcgatcac ccgatttcag
     2761 accgaacctc ctacccaggg aagagccccg agcagccccg gcggtggcag ccggcgaggt
     2821 gggtgtcgat cagcccgatc gcctccatca gcgcgtgcgc cgtagtcggc ccgacgaaga
     2881 cgaacccctt tgtcttgagc gccttcgcca gcgccttcga ctccggcgag acggtcggca
     2941 cctccttcgg cgtgcggggc atcggtgtgg tctccggctg gtgggaccag accagcgtat
     3001 ccagcccgcc ctcgtcgcgg agcgccacgg tcgcccgggc gttggtccgc gccgcctcga
     3061 tcttggcgca gttgcgcacg atggcggtgt tatccatcag acgcgcgaca tcggcgtccg
     3121 taaaatccgc gacgatcccg gggtcgaacc cggcgaacgc ctcgcgaaag gcgtcccgct
     3181 tgcgcaggat cgtcagccag ctcaggccag actggaacgc ctccagcatg atccgctcga
     3241 acaggccctg ctcgccccgc acgggcatcc cccactcggt gtcgtggtaa tgcaggttga
     3301 gcgggtgcga ggtcgcccac gggcatcgcg ccaacccatc ctctcctgta accacgccgc
     3361 acatcgccgt gccgatctcc gtcatcgttg catcactcca gcgctgtcag ctgtatatcg
     3421 atggttcgag tgtatcgatc gtgtggccag gctccgtccg ggtatcctgg ttgactcggc
     3481 cgccttgatc gaggaacacc tcgatgcccc gggggcgtgg tctgctcgag tctggacctg
     3541 cggtttcgcg aggccgggca gcggatcgtg gtatttcctg catcgggctg gaaaggactg
     3601 aacgggatca gcaaaatgtt gacgtgtcat gcgcgcacca cgcaggggat tgaggaggac
     3661 ggtgaatgcc tctggtgaag cttgtttcgg caggcattca cagacacctt ccggatcggt
     3721 gccctcctcc ccaaaacggc gatcgagcgg cgacgattcc tccctatcga ccggttgccg
     3781 ccgatgatcg gttcccccac accgccacgt ggtgaccgca gcgctccccc tcccacagcg
     3841 gcaccgtgca cgtcttgcgc agcaagttct tccagagctg ggcgttggcg aaggtctcct
     3901 catcggcgct gaagacctcg aagaccgatc gcgccgccac caacaccagc ttctgtttcg
     3961 cccggctcag tgccactgtc agccgccggg gatcgagcag gaactccccg ttcatcagga
     4021 tgtagtcgcg atcgctctcc gtcaccccta tcaagatcgc atcgcgctcg cctccctgga
     4081 agcgctcgat ggtgtcgacc gccgagacca cggcctcgtc ggcgtcgctg tgcggaccca
     4141 gcagccgcac caggtcctcc tgcagggcag cgcgctgggc tcggtgcggc accaccacgc
     4201 ccagcccctc gtgtgggtcg agcgcgaagc gcgccgggtc ggagagcgtc tgcagcatcg
     4261 gtgccagcaa cctgcgctcg aagtcgttgc ggacctggct gttcgtctcg tcgtggacca
     4321 ccaccgtcaa ggggtgctca ggagccagca cggcggcgag gaagtcatcc ggtgtcggat
     4381 agtccgaaaa tagatttgcc ttgtgcgagt ggtactcgat gccgtcgtgg atgtagatct
     4441 cgtcccgaag gaacttcgcc atgtcggcgt gcagccggta gctctcggcg aactggatca
     4501 tcggcgggtc aagcgccagc agagccagga agagcgactc aaaggtgcgg aactgccgga
     4561 acgaccgccg cggctcgctc gcccagtcgt gcctgacgat cggtggcatc tgccggtggt
     4621 cgccgaccac gatcacgctc ccgcccggct tgagcggcag cgccgccata atcgcctccg
     4681 gcatgctcat ctgcgaggcc tcgtccagca cgaggcaatc ggcaagagga tgcccgaagc
     4741 ggtctttcga gccgaagtga tcgccgagca acgcgtagat gccacccggt gtcgctgcca
     4801 ccaccgccca tcgattcgcc agcagccggt cgaccgcctt cgcctggccg tcctggcgtg
     4861 ccttgtctct cggcagcggg gtgacgccag ccgggacatc gttcttgctc cgatagcgat
     4921 agagcggcag gtcgagcacg cgctcgtcga tgtgccggcc gatgatctcg gagtgggtcg
     4981 tccgcagctg ccggagcagc tcctgcgcct cggcgacgtt ctcaagcagg acatcggtcg
     5041 ccgcgtgcgt cttgcagctg agaaagacac gatactcacg attcgccgcc atcgcaccct
     5101 ggatgcgcgc gaggatcgcg taggcggtcg agtagctctt gccggtgccc ggcgggccct
     5161 gcaccagcag caccggcgta tcgccgtgcg caccgatgaa ctcgcgcttg ctcaactcga
     5221 agtcgtacac gttctctgcg gctgcggcat ggagcgcgtc gatcccatcg aggaagcgct
     5281 gctgtgccgc cctggctgcc tccggccact cgggctcggg gaattcccgc cccgcgagga
     5341 tgtcgtagag cgagttctcg ccgccggcga tcaacccttc ggtcacctgc ttcgtccagg
     5401 cggcatagat gttgctcggg tcggggtcga gcgtcaggac ctcaccatgc gtcaatggcc
     5461 ggtcgttgtc gccgatcgtg ccgaaggtga atcccgccat gcccccaccg cgcgtgccgt
     5521 tcatcgtgac ttcggcccac gcccactgcg ccattccctg ctcgtcccga tcgacaacga
     5581 tgcgctcgag tgtgcagcgc atcccgtaga gcagctgctt ggcggtcgga gtgaacaggt
     5641 agcgctcggc ctcaggcagg cggctgtcga aggtccagcg cgggaagatc accaaccagt
     5701 ccccctcgcg gatcgtcgtc agacgcagcg cctcgtcgag atcgcaggcg acgtcatcca
     5761 ccgtgatccg caggcggaag cacagcccct cattggtcca gtcgctcttc gccctctgtt
     5821 ccttcggcag ccggacctgc tttgcgtcgg ggtgcgcttc gcggtacctg gcccggtact
     5881 cctccttcat ggcccggcga tggacgttgt cccggttctg cgcggccatg cccggctcct
     5941 gatcctcctc gcggtactgc acgatcagcg tctgcccgct cagcacgcgc tgctccggct
     6001 cggcgaggcg gtcgcgcttc cactcggcca gctcgacgtg ccgctcgatg acgacgaact
     6061 cgttgagggc atgggcgagg tcgcgggcga tctcctcgaa cgcggcgagg tccggcagct
     6121 cgaactcccg cagcgcggtc tgcctgttgc cggggaggtc ccggtcgacg atatgctcca
     6181 tcgcctccag gcgccgcgcg tggaatcgct gcagcagctc cggcgtcgag ccccggtagg
     6241 tggcgaagtc gtcctccccc tcctcgggca ccggcaactc accccacgcg gcgtaggcgt
     6301 actccagcgg gatctggctg ttgaagcgcg cccggctggt gaaccagcca tccttgccgg
     6361 ggatctcctc ggccccgtcc ggcggggcct cgaaccggcc ccagaagtcg aacattcgcg
     6421 tgcggaagat ctcgcgatag ggcgcgccgg cgttccagtc gaacttgagg taggccgcga
     6481 cggcctgcag cgactggcag accatcgggt agttacggtg ctcgcgaatc tccgtctcaa
     6541 gatagctggc gatcgcggag tcgaacgccg ccagctgcgt gacgaaatcg tagagggccg
     6601 tcgcaccgag gatgctgtcc ttgtgtcgtg cgagaccgtc cagcagcacg cgctgggcga
     6661 atccgttgac gaaggtgagg tggatcggcg ccttcgcttc cccttcgatg ttcggtgcgg
     6721 cgagctcgac gatcgtgcgc agcgtttcgg tgatccagtc gacgaagagc gactcttcgc
     6781 gctcatcgga gtcaggcggg ccgtcgctga gccgagcgat gctgcggcgg cgtttcgggt
     6841 gcggtcgtcc gccctcgttg gcaaccacca gtgcgccgag catgtaagtg cggtcgttga
     6901 ggtagtcgtg ctgggcatcg atgaagattc gcacgaggtt ggggttctgc tcggcgtcgc
     6961 tgtgcggcat cgagccgtag ccgcggttcg gaatgtagct gagagccgtt ccctgcttcg
     7021 tcttccagtg ctcgtagcgc agcgcccggt ggatgagctc gtccagtctc gggccgaccg
     7081 gccacgttgc cgcgagccgc tcgcaggtat cgctgtgacc cggactcggc accaggatcg
     7141 tgcgctcgtg caccttgccg tcgggaccca tctcgcccgg cttgaccaga gacttgagcg
     7201 aggccagttg ccgggtggtg gcgatgccgt tgtcttcgag cgcgcgcttg tcctgctccg
     7261 tgatgtgggg cagcagcgag agattgtccg tctcggcgct gcgcttcatg cagaactcgt
     7321 tgtagagaca cccgtcgcac ttccaggtga ggtggaaggg gatctcctcg aagtcggtcc
     7381 caagcacgtg gtgagcggtc gattctggct tggtgacaag atcgcggacc gagccaaggt
     7441 aggcgtcgac gtcccgaaca cggtcgagga gtcccgccga ggtgccgagt gtctgttcgg
     7501 cgtcctgccg ctggcgctcc tggtcctcgc gatcctccac cggtgccagc aatgattccc
     7561 ggaccggccc acggtagagg atcgccagct cgatcggcgc gtgcgcgatg cccgcctcgg
     7621 ccagcagcgt ggcgagcatc tcgtggtaga aggcgacctg cagccggtgc tcgacacggg
     7681 aagccgtgct gctcttcatg tcggcgatga gcaggcgcag ggtgtcggcg gcatcccgct
     7741 cggcacggag gagatcgaca tcgccgcgca gccgccagcc gtcgaccgtc gcctcgacgc
     7801 gcggctggaa gagcacgacc gtctcccccg ccccgagcgt ggcgagggcc tccatggtgt
     7861 cggcgttgtc gtgggtgaga tgcagctcac ggcggcgctc ggggccgaag cggagagttg
     7921 ggtattgttg cgcgatatct gcctcgaccg cgctctcgaa gctcgcgccg gaccgggtca
     7981 ggatcggcgg gatcgactgc ggcgcgacgt cgtaggcgta gaggaagtca cgcccctcgg
     8041 ctcgctcctt gagacgcagg cggagatagc gctcgcactg ctccaaccgg atgaactggg
     8101 agatatcggt tggggagatt gagcgcggcg cggcggcgag gttgccagcg cgggcgtcgg
     8161 gtcggcgcgt gatgtccatc ggggtgttcc tgaccggtcg ggccgtgcgt tcgtgctgca
     8221 agtctagccc cgactggcca tttccggcga gatgcagggc gacgatgtca ggcggtgctg
     8281 acgggcggca ggttgcgaaa cggtacgttc cttgcgttgg actccagtac gccccggcac
     8341 gtttaaaccg ggagatccac agtttcggaa ggagccagca ccatgatgcc ggatgaccac
     8401 gatcagcaat cggaaacaga cctcacctgt gaaacatcgg ccatggaggt gtctggccgg
     8461 ctatcccggc gcgagatcct gggtcgctcg atccttggga tcacgggcac cgggctcgtg
     8521 tccctcctcg cagcctgcgg gggtgaagag gaggacgagg ctctggaaga cgtcgacgat
     8581 caggaagagg acgaagccgc cgacgtcgag gaggacgaga accccaccgg tatcgaggat
     8641 ctcgacgagg ctcccgccgg cgaggatccc gcgcctggag gcaatggcgg ctgacgaggc
     8701 caggacgccc agatggcgag gcggttacgg tgaccatgtg cagtagttct gaccgggttc
     8761 aaccacccgc cacgatgact cacttgcgtt ccatgccgct gcggtggttg catgcggaaa
     8821 gaagagcata tcaaaccacc taacgggaga ggcgggggaa tttcaccagc ctaacccgtt
     8881 aggtgtggtg tgcgatggta aggtatgcgc cttgcgggaa tccaacagga ttcagcgaca
     8941 tgcattcgcg ccgactatct gcatgaccgg aagggccttg aatcatgatc cagcttcagc
     9001 gcaatgaaca cctgaatatc gatgaggaga tgacgctgct ctcccgtcgc gcgatgctgc
     9061 gccgttcggt cctcggtatc accggcgtcg gcctgacggc cctgctcgcc gcatgcgacg
     9121 atgacgagga cgacgacgaa gatgccgctg gcgagggcat cgacccagag gatgccatgg
     9181 acagcattcc gctcgacccc gacagccccg ccatcgacga agaaaccgat gttgtcccgg
     9241 aagaggttat cccggaagac gcggaggagg aagcagacac ctagagcgcg ctgcagccaa
     9301 gtatccgctc ctccacgatc agatggccac gctgcaatcg cgctagaaga tcccgaggtc
     9361 gatgagaccg agtgccgaga gcacggcgag gaggagcggc tggtgcagaa ggtagatggc
     9421 gagcgagtag cggccgactg tcgccagctg ccggccaggc cagggcggtt gcccagccgg
     9481 ccaggggaag cgccgctccc catttcggta gagggagctg ccagcgacga tgcccagcag
     9541 gacgacgccg aaccagggca tcatcggacg gtaatcgagc atgtacccga ccggcgcgcg
     9601 gacgccgagc cagagcagcc acggctcatc catgtgcagg gaccgcacga ccggcgccgc
     9661 gagcaccacc gggatggcga ccagcagggc aagtgttggc cgccccagga acgcgtaggc
     9721 caggatgatc gagaggccga tggcgtgcag gatgccgaag tagatattgc cgtccggcac
     9781 ggcgagcccg atcaccagcg tgatgagcat gccgtacccg agaattcgca cgccgcgcag
     9841 ggtgtacttg cggaacagtt gagcctcgct ggcagtcgtt cggcgcgagc ggtcgtagct
     9901 cacccgcagg gaaaccccca ccagaaagac gaacaggaac gcggtgatcg tcgcaaagtg
     9961 gcgccaaaag ccgcgggtag cgttgagatc gaagcctccg aagcgatcca gatcgaagac
    10021 gaggtggtag agcaccatca tcgcgatggc gatgcctcga acggtatcga tctcccagaa
    10081 ccgtggcgcg gcgagtgcag tcggttgaac ctcgggccgt gcgcgctgtc gcgcctgcca
    10141 cctggccctg gtgtcggtat cgaggggctc tccgcccata cgggctccgt ctcctgtttc
    10201 gtcgatcggt cagtccagga aaggctgggt gtacgggact ctcgcgtgga tgggtcgctt
    10261 gcggcggttg gcctgggatc acgagcgccg ggctggcctg ctgaagggat tcgcttgcct
    10321 ggatcatgct catgggaaag aggtctttcc cgtttccagt atgcaatgcg gttcatgatc
    10381 gtctcgccgc accgacaggc tccggatacc gccgagcggt gtaccctcgc gatcggtgag
    10441 cgtcacccgg ctggcgctgc atcggaactc cgcatcacac ccctgcccca cgccgattga
    10501 cagatgggcg tagacgcggc tcctgttgag gaagaagctg ggcggcgcgt ccattgacct
    10561 tccggacgag acctccattg aggtgctcga cgccatgggt tggttccagg attgacacgt
    10621 tcgatccgac ctcagggagc aggccggggt ctcgacgggt tggttatcct gagatcacgt
    10681 ggccgtccgg tacgcttctt gcggacatgg tgacatgact ggctgcgtcg atgccggaga
    10741 ttgtgttttc ctgttttgtc ttgaaggact cgccaccatg cccctcgatc accgaaccga
    10801 ccttctggat tccgacgagg aaccgaccct gctctctcgc cgcgggctgc tgcgccgctc
    10861 ggtgtacggc atcaccgg
//
LOCUS       DASYWL010000234        13869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_199222, whole genome shotgun sequence.
ACCESSION   DASYWL010000234 DASYWL010000000
VERSION     DASYWL010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 13869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13869
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199222"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1036
                     /locus_tag="VGR22_08630"
     CDS             <1..1036
                     /locus_tag="VGR22_08630"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV2128668.1"
                     /translation="NDGCMSPNQAVQFVIGASTPMEYATLDRIPAKFSRIVQGTVMIS
                     DEDQTAADALLDAVWENGGRTFDTAHGYGRGACERALGSWLSRRGIRDEAVIIGKGAH
                     PYDGRNRVTPENITSDLHESLERMGIETIDLYLLHRDDPTIPVGPIVDVLNEHLRAGK
                     IRRFGGSNWTTIRIAAANAYAAESGLKPFTASSPNFSLAQQRKAPWAGCISVSGEAGA
                     AEREWYITHDMPVFPWSSLAGGFFSGRFTRTNLHTFRETLDLLAVEAYAFEDNFRRLD
                     RVQEAAEWHEASVAQIALAWVLHQPMRTFPLVGSRTGEEFAANAAALDLVLTPEEIEW
                     LDLQRDERPW"
     gene            1174..1755
                     /locus_tag="VGR22_08635"
     CDS             1174..1755
                     /locus_tag="VGR22_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128669.1"
                     /translation="MRVGRHTVGLSSIVTVFIALALALGAMLAFTPAVSAQEIAFRVT
                     KYTCETDPGQISPIADNAPEDCELTEGVSFSIQVVGTDTVLQCATDATGQCSVQVESE
                     SEVIVTEDISTVPAGYSPRDNPIETTAVTEFAGAVFVNLPTGNGNGETPTELPETGSG
                     DTVIATSGAAGMLTVIAVVALLGSRIARRTAVH"
     gene            1844..2557
                     /locus_tag="VGR22_08640"
     CDS             1844..2557
                     /locus_tag="VGR22_08640"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEV2128670.1"
                     /translation="MGDTSRRDSTLERVTVIGLGRFGTSLVRHLYEIGYDVIAIDINQ
                     EHVDVIQRHRDALMAVRADGTDEQTLRDLEVDRSEVAVVAQGENLEASVLTTLLLKRI
                     GVPFVVSKAKTDLHGELLSKVGADQVIFPEKDAGRRLAHSLGARVVSDYITLSANAGI
                     AKLTAPEGFVGSTIGDLVGYRSQTINVLVIRRGNNLINLPSFQEVIRAGDEIVVVGGD
                     RAINAFVSADPVNARDKRT"
     gene            2567..4057
                     /locus_tag="VGR22_08645"
     CDS             2567..4057
                     /locus_tag="VGR22_08645"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HEV2128671.1"
                     /translation="MVSPGPHRQRRPGDRVIRRRVDAQQVMELPPPPRRRPVPTARSN
                     AKVFALAYIMLVAIGTVLLSSPLASESREWTPLVDALFTAISATAVTGLVTVPTQEHW
                     SFFGEALILLMIQAGGMGFMVGASLVLITLRRSSSLRGSLIMRDGAPTLSLSEARMLT
                     GRILRFMLVVEAIGATLLAVEYSPESDGIFDAIWWGTFHAVSAFCNAGFDLQSGGRSL
                     YGFRESFLINLTVMSLVQLGALSFMFFADLWQRKIWLSPVHWHPRRFWYELQMDSKLI
                     LIANYSLVLFGAIAFMIIEWNTGLSHIPWALDKVVASFFQSVSARTAGFATISFSETQ
                     SGTLFLWLGMMLIGGAAASTAGGVKLATFSVVVLAVLSTVRGQVEPQFGGRRIPTVLV
                     FRAMTIIAIFLIVHFFLTLSLVFTEDVLNREDFSFLSLMFETMSALATVGVSTGLTGD
                     LSSAGKLVLCVAMFVGRLGPLTAVYALQRRESRQDYRYPEAWVRMG"
     gene            complement(4128..4820)
                     /locus_tag="VGR22_08650"
     CDS             complement(4128..4820)
                     /locus_tag="VGR22_08650"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEV2128672.1"
                     /translation="MASTTIGPIPAEALAFLKSVDSPTIANAVETFEVRDRTEGFIGG
                     RVQCQFPDLGVMVGRALTVTMSNAPGAVAPRDGYWQMWDALAAAEGTVVIAIADSSGE
                     PHRVAYAGEVMATIAKRLGAVGMVTDGALRDVDEVHAMGFHYFMQYPVVSHANFEITS
                     VGEPITLDGEDIRTGDILHGDRNGIVVVPDGVLEGLSMAVEGIREKEAATMAYVRGDE
                     FDYAELKQRSGY"
     gene            5469..5834
                     /gene="ndhC"
                     /locus_tag="VGR22_08655"
     CDS             5469..5834
                     /gene="ndhC"
                     /locus_tag="VGR22_08655"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEV2128673.1"
                     /translation="MEVYGENWIVVLFMVVTATFMGLLLLTLNQIMAPSKPSKLKSEP
                     YESGMPGVTPVKAQFTPRFYVIAMLFVVFDIEAVFIYPWAVIFDDLGLFGFVAMAVFM
                     FLLLDGYIYAWKKGALEWV"
     gene            5825..6376
                     /locus_tag="VGR22_08660"
     CDS             5825..6376
                     /locus_tag="VGR22_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEV2128674.1"
                     /translation="MGLIDDRFDKNVFVTSMDFLFNWGRRQSLWWLQFGLACCAIEMI
                     AASMSRWDIPERWGMLFRASPRQADLMIVSGTVTKKMAPIVRTLYDQMPEPKWVVSMG
                     SCANVGGPYDTYAVVQGVDQLIPVDVYVPGCPPTPEALYYGILQLQDKIIRYGTMARK
                     RGVDAAEEQRRADRSEAMAGVRG"
     gene            6416..7171
                     /locus_tag="VGR22_08665"
     CDS             6416..7171
                     /locus_tag="VGR22_08665"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEV2128675.1"
                     /translation="MSDGDIRDNQNVTDEGTIGAPGSDQPTQDQGQSPASYKDKRSDE
                     ADHVEELEQDLDLDTHDVDWSREAYYAEDLERHPAVRVLSASLPGAIVDIIRFRDETT
                     IHVQPELFREVCRTLRDDENVAFDFLTDLTAVDMLRLRTSPRFDVVAQLYSLRNRMRV
                     RIKAGVDDGQSVPSLVPLWNGANWMEREAYDMFGINFDGHPNLKRILLPDDWDEGHPL
                     RKDYPVRGWKEFPVYNTERTVPRLRTRWTGRGV"
     gene            7177..8493
                     /gene="nuoD"
                     /locus_tag="VGR22_08670"
     CDS             7177..8493
                     /gene="nuoD"
                     /locus_tag="VGR22_08670"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEV2128676.1"
                     /translation="MASTTGRPANDDIRDIEQSPYGKPGTVIDETTPPIGTLTIEGER
                     TLDTGEREMTLNMGPQHPSTHGVMRVLLKLDGEIISEADPVIGYLHRGTEKLGEIKRY
                     AQITPWTDRTDYVAAPSNNLGYVLAVEKLCGIEAPERAQYWRVIMAELTRIASHLVWL
                     GTHALDIGALSMSLYCFREREIILDIFESFCGARLTTNMMEIGGFSRPIPDGLAGQVR
                     NFIRVFPGRQAEYDALLTANPIWMARTKGVGIISPEAAISYSLTGAMLRASGINYDVR
                     KAQPYLVYDRLDFEVPLGEHGDTYDRYLVRMEEMRQSVRIIGQALNYVEHMDTPLANF
                     ESPYVIPPHKGTMTTAEDMQRHFVWTIKGFNPPVGEVWQTIEHSKGELGYYLVSDGGP
                     MPYRFRIRTPDFVNLQALPHMAQGALLADVVALIGTIDIVLGSVDR"
     gene            8687..9727
                     /gene="nuoH"
                     /locus_tag="VGR22_08675"
     CDS             8687..9727
                     /gene="nuoH"
                     /locus_tag="VGR22_08675"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEV2128677.1"
                     /translation="MDQPIWITFIVAFVILNVLLISMAYMTWFERKVLAAMQDRLGPT
                     RTGWHGLGQPIADAVKLLGKEDLVPAGADKIVFRFAPVIVFVAALLSAAVIPFGGTIE
                     LFGQEVNLFITDLNVGALFILAVGAAGVYGIILGAWSSANRYSLLGGLRSAAQVISYE
                     IVLGLSLVGIFILTGSLSLLDILERQAETIALGPLEIPNWFILSQPLAFILFIIAVVA
                     ETNRAPFDLPEAETELVAGFHTEYSGFRFSLYFLGEYINMIVASLFAATLFLGGPNGP
                     GSDEYWGLGLLWLFLKALIFLFFYVWLRGTIPRFRFDQLMGIAWKVLLPLALANIIIT
                     AFIRLVANGEVF"
     gene            9824..10546
                     /locus_tag="VGR22_08680"
     CDS             9824..10546
                     /locus_tag="VGR22_08680"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEV2128678.1"
                     /translation="MPWGVIFFYLIATVSILSAIGVVAARNPVHSSIFLVLCFLNVAG
                     IFVMLGAEFLAVIQVIVYTGAILVLVLFVLMLVNPDELPSFTDAQPLQRYLGLLLGAV
                     LLLEVGAAIITRTALEGVGEATPENVAAVGGNVHAVGRVLFSDYLLAFEVISIVLTVG
                     ILGAIVLAAPERLGEKLATRRGTISLSHTRGTDAELPVGPRGETPIKTDPNRRPPRPE
                     APRRVIKVTDPDERTTIGGTRR"
     gene            10582..10890
                     /gene="nuoK"
                     /locus_tag="VGR22_08685"
     CDS             10582..10890
                     /gene="nuoK"
                     /locus_tag="VGR22_08685"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEV2128679.1"
                     /translation="MAELGIEHFLFLSAALFVIGMTGLLTRRNLLVIFMCVELILTSV
                     NISLVAFGWETNTLNGQIFAIFAISIAAAEAAVGLGIILALSRRIRSVDVADIRELRD
                     "
     gene            10958..12883
                     /gene="nuoL"
                     /locus_tag="VGR22_08690"
     CDS             10958..12883
                     /gene="nuoL"
                     /locus_tag="VGR22_08690"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEV2128680.1"
                     /translation="MEDFLYLIPLLPLVAFAINLLLGRNWIRGNAHWVALPLVAASWV
                     VALLAFWEVYDTEHAISQHLFTWIPSGNFVVDVNLYVDALTAVMLLVVLTVGLLVHVY
                     SVGYMHGDPGFYRFFAYLPLFVFSMLMLVLADNLLVLFVFWEAVGLCSFLLIGYYFRR
                     RSAGNAAKKAFIVNRVGDLGFGLGVILTFWTFGTISFYGEEGIFEQAINHTEFGIGEG
                     TLAAIGILFFIGATGKSAQFPLHVWLPDAMEGPTPVSALIHAATMVTAGIYMVARNYT
                     IFIQSETAMLVVAVVGAFTAFMAATIALTQNDIKRVVAYSTVSQLGYMAFALGTGAWV
                     AAIFHLMTHAFFKGLLFLGAGSVIHGMHEEQDIRNMGGLRKHMPVTFWTFLIGSLANA
                     GVIPLAGFWSKDEVIVGAWISESWFGEIAAVIGLVAAFLTALYMFRLVFLVFFGEERF
                     DTEHLHPHESGGWMAWPLILLAIPSVLIGFVGFPPEDGPFHHFVHHAFEFVEVHHPST
                     TMLITFGVISTAVALGGIFVAWLVYMKGAINPASVSEKYEGLYEFLLNKWYFDELYDR
                     LIVQPLRAFSDFLWRVVDEGIIDAAVNGVAVGIAAVSQRVRHVQTGLVANYALAIALG
                     MILLIGAFIAAYSNLFR"
     gene            12959..>13869
                     /locus_tag="VGR22_08695"
     CDS             12959..>13869
                     /locus_tag="VGR22_08695"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEV2128681.1"
                     /translation="MDFPLLSTIAYLPLVGALAILFAPRASSELPRTIALVASLAAFA
                     VSLVMLFSFDRNAEFQFVEQVVWLDDLGVSYFMGVDGIAVLLIALTTLLSVIAIWWSW
                     ATIRERTREYYIAMLLLETGMLGVFMALDLFVFYIFWELVLIPMALLIGIWGSTDRVY
                     AAVKFFLYTLFGSLLMLVAIVATYQSYFNETGIRTLNVLELQAGTYGTTFQFWVFAAF
                     FLAFAVKVPMFPFHTWLPDAHVQAPTAASVILAAVMLKMGGYGLLRFNLPLFPEGTDA
                     WSVAIIVLSIIGIIYGALVALVQPDMK"
BASE COUNT         2352 a         4597 c         4244 g         2676 t
ORIGIN      
        1 gaatgacggt tgcatgagtc cgaatcaggc cgtccaattc gtgatcggag cctcgacacc
       61 catggaatac gcaacgcttg acaggatccc cgcgaagttc tcgcgcatcg tgcagggcac
      121 ggtgatgatc tccgatgagg accaaaccgc cgccgatgcc ctcctcgacg ccgtctggga
      181 gaatggtggc cgcaccttcg acacggccca cggctacggc aggggcgcgt gcgagcgcgc
      241 gctggggtcg tggctctccc gtcgcggcat ccgcgacgag gcggtcatca tcggcaaggg
      301 ggcgcatccc tacgatggcc gcaatcgggt cacgccggag aacatcacca gcgacctgca
      361 cgaatcgttg gagcggatgg gtatcgagac gatcgacctc tacctgctcc accgcgacga
      421 ccccacgata cccgtcgggc cgatcgtcga cgtgctcaac gagcacctgc gggcagggaa
      481 gatccggcgc ttcggcggat ccaactggac gacgatccgg atcgctgcgg ccaacgccta
      541 cgccgccgaa agcggtttga agccgtttac cgccagcagc cccaacttca gcctcgcaca
      601 acagcgcaag gcgccgtggg caggctgcat cagcgtcagc ggcgaggccg gcgccgccga
      661 gcgcgagtgg tacatcaccc acgacatgcc cgtcttcccg tggtcgagcc tcgcgggcgg
      721 cttcttctcc ggccgcttca cccggacgaa tctccatacg tttcgggaga cgctcgatct
      781 gctcgcggtt gaggcctacg cgttcgagga caatttccgc cggctcgacc gtgtgcagga
      841 agcggctgag tggcacgaag cctcggtcgc gcagatcgcg ctggcgtggg tcctgcacca
      901 gccgatgcgg acgttcccgc tggtcggcag ccgcactggc gaggaattcg ctgccaacgc
      961 cgccgccctc gatctcgtgc tgacaccaga ggagatcgag tggctcgacc tgcagcgcga
     1021 cgagcgcccc tggtaagccg gccaggccga gcgctcgacc tactatgatg catttactgg
     1081 cggtgacata accggccgct ggaacgatcc gggaccgtcg ttggatgtat cgacatcaag
     1141 catgcacgag ttgcatggga aaggatgtgc atcatgcgcg ttggtcggca cacggtagga
     1201 ctcagttcga tcgtgacggt gttcatagcg ctggcgcttg cccttggcgc gatgctcgca
     1261 ttcacgccag cggtgtcggc gcaggagatc gccttccgtg tcacgaagta cacctgcgag
     1321 acggaccccg gccagatctc gcccatcgcg gacaacgcgc ccgaggattg cgagctgacc
     1381 gagggcgtct ccttctcgat ccaggtcgtg ggaacggata ccgtcctgca gtgcgccacg
     1441 gacgcaaccg gccagtgcag cgtacaggtc gagagcgagt ccgaggtgat cgtcaccgag
     1501 gacatctcga ccgtgccggc cggttactcg ccgcgcgaca atcccatcga aacgaccgcg
     1561 gtgaccgagt tcgccggcgc cgtgtttgtc aacctcccga ctggcaacgg gaacggcgag
     1621 acaccgaccg agctgcctga aaccggaagt ggcgacaccg tcatcgcaac ctccggtgcg
     1681 gcgggtatgc tcaccgtcat cgcggtggtg gcgctattgg gtagccgcat cgcgcgccgc
     1741 acagctgtcc actgatccct catttcgctt cacagcaatc gctgccgaac cgccccggaa
     1801 acgggccgga gcaccgtacc aaccgctgga agggaagcaa agcgtgggtg acacaagtcg
     1861 ccgggatagc acgctggagc gcgtgaccgt catcggactg ggccggttcg gcaccagtct
     1921 ggtgcgtcac ctctacgaga tcggctacga cgtcatcgcc atcgacatca atcaggaaca
     1981 tgttgacgtc atccagcgcc atcgagatgc gctgatggcc gtccgtgctg atggaacgga
     2041 cgagcagacg ctgcgtgacc tggaggttga ccgctcggag gttgccgtag tcgctcaggg
     2101 cgagaacctc gaagccagcg tgctgaccac cctgctgctg aagcgcatcg gcgtgccgtt
     2161 cgtggtctcg aaagccaaga ccgatctcca cggcgagctg ctcagcaagg ttggcgccga
     2221 tcaggtcatc ttcccggaga aggacgccgg tcggcggctc gcgcactcgc tcggtgcccg
     2281 cgtcgtcagc gactatatca ccctctcggc caacgccggc atcgccaagc tcaccgcacc
     2341 ggagggcttc gtcggcagca ccatcggcga cctcgtgggg tatcgatcac agacgatcaa
     2401 tgtacttgtg atcaggcgcg gcaacaatct catcaacctc ccctcctttc aagaggtgat
     2461 tcgggcaggg gacgagattg tcgtggtcgg tggagaccgt gcgatcaatg cctttgtgtc
     2521 cgccgatccc gtcaacgcac gagacaagcg gacgtaggcg ggcgagatgg tttcgccggg
     2581 cccacaccgc cagcgccgac cgggtgacag ggttatccgg cgacgcgtcg acgcccagca
     2641 ggtcatggag ctgccgccgc caccgcggcg ccgaccggtt cccaccgcac ggagcaacgc
     2701 caaggtcttc gccctggcct atatcatgct cgtcgcgatt ggcacagtcc tactctccag
     2761 ccctctcgcc agcgagtcac gcgagtggac accgctggtc gacgccctct tcacggccat
     2821 ctcggcaacg gccgtcaccg gtctcgtcac cgtgccaacg caggagcatt ggagcttctt
     2881 cggtgaggcc ctcatcctcc tgatgatcca ggccggcggt atgggcttca tggtcggagc
     2941 ctcgctggtg ctcataaccc ttcggcgcag ctcaagcctg cgcggtagcc tcatcatgcg
     3001 ggacggcgcg ccgacactct cgctcagcga ggcacggatg ctcacggggc gcatcctgcg
     3061 cttcatgttg gttgttgagg ctatcggcgc gacactgctt gcggtcgagt actcccccga
     3121 gagtgacggt atcttcgatg ccatttggtg ggggacgttc catgctgtat cagcgttctg
     3181 caatgcggga ttcgacttgc agagtggcgg cagatcgctg tacggattcc gggagtcgtt
     3241 cctgatcaac ctaacagtca tgtcgctagt ccagcttggc gccctctcct tcatgttctt
     3301 cgcagacctc tggcaacgga aaatttggct ctcaccagta cattggcacc ctcgtcggtt
     3361 ctggtacgag ctccagatgg attcgaagct catcctcatc gccaactact cactcgtgct
     3421 cttcggtgcg atcgccttca tgatcatcga atggaacacc gggctctccc atatcccttg
     3481 ggcgcttgac aaggtggtcg catcgttctt ccagagcgta tcggctcgta cagctggctt
     3541 tgcgacgatc tccttcagtg agacgcagag cggtacgctc tttctctggc ttggaatgat
     3601 gctgattggc ggagcggcgg cctcgacggc tggcggcgtc aagctcgcca ccttctcggt
     3661 ggtggtactt gcagtgctgt cgaccgtgcg tggccaggtg gaaccgcagt tcggcggacg
     3721 ccgtattccg acagtgctgg tgtttcgcgc gatgaccatc attgcgatct tcctgatagt
     3781 ccacttcttc ctgacgctgt cgctcgtatt cacggaggat gtcctgaaca gggaagactt
     3841 cagcttcctc tcgctcatgt ttgagacgat gagcgcgttg gccaccgtcg gagtcagcac
     3901 cggactgacg ggagacttga gctctgccgg caagctcgta ctctgcgtgg cgatgttcgt
     3961 cggtcgcctc gggccgctga cggcagtcta cgcgctccag cgccgcgaga gccgtcagga
     4021 ctaccgatac cccgaggcat gggtgcgaat gggctgagcg cgccgccgcg tacgacagtc
     4081 ggaaagatca tcgaggcggc cccaccggtg ggccgccctg atgccgatca atacccgctg
     4141 cgctgcttga gctcggcgta gtcgaactcg tcgccacgga cgtaggccat cgtcgccgcc
     4201 tccttctcgc ggatgccctc gaccgccatc gacagcccct ccagcacgcc gtcggggacg
     4261 acgacgatgc cgttgcggtc cccgtgcagg atgtcgccag tacggatgtc ctcgccgtcg
     4321 agtgtgatcg gctcgccgac ggaggtgatc tcgaagttgg catgggagac gacggggtac
     4381 tgcatgaagt agtggaaccc catcgcatgg acctcgtcga cgtcgcgtag ggcgccatca
     4441 gtcaccattc cgaccgctcc gagccgcttc gcgatcgtcg ccatcacctc gccagcgtag
     4501 gcaacacggt ggggctcgcc gctggagtcg gcgatggcga tcaccacggt accctccgcc
     4561 gctgcgaggg cgtcccacat ctgccagtag ccatcacgcg gagccaccgc accaggcgcg
     4621 ttggacatgg tgaccgttaa cgcccgcccg accatcaccc cgaggtcggg aaactggcac
     4681 tgcacgcggc ccccgatgaa gccctccgtg cggtctcgga cctcgaaggt ctcgaccgca
     4741 ttggcgatgg tgggactgtc gacgcttttc aggaacgcga gcgcctcggc cgggatcggg
     4801 ccgatcgtcg tggatgccat ggattcctcc cgcgcgtagc ggtcacggca cacgcgaacc
     4861 gtgactcgct gtcagatcga ttcccgagga tagcgtgcgc cgcagattca cggaagtgcc
     4921 atgaggcagg tccggcgcgt gggaacctgg gagtgccaag ggacacagga gagcgggtat
     4981 tgctgccgtt gcgggaaact caggccgtga acacaaagta accgcccggg ggatggcccg
     5041 agcggttaca gacaccacat tttggaggga agggggaaga tcacaccgtg gctggcgggg
     5101 cgatctctat ggtgctgatt ccagtttagg tgtgccagaa cagcacagcg gttccaatag
     5161 gatacgatgc aaggcacgtt ggggactgat cccgcgaccg agggattatg cgcacgacct
     5221 gcgccatttc accctgcact gactgcccgg aatggtcgtt cttccgtgcc agtaggccca
     5281 gatgcgtgtg gtatcttgtt cgaggtttgg ggcaggctcg gggagacgcc tgccactcgc
     5341 atccggcgga aatcggggcc atatccggac ccacgtcata acagccggat gcttcacgct
     5401 ggccgatcac ggcacggcca ctggccaccg accacaatgc agtctcgcac gcggggagca
     5461 gaatttccgt ggaagtctat ggtgagaact ggatcgtcgt cctgttcatg gtggtaacgg
     5521 cgaccttcat gggtctgttg ctgctcaccc tgaatcagat catggcgcca tccaaaccgt
     5581 cgaagctcaa gtcggagccc tacgagagcg gcatgccggg cgtcaccccc gtgaaggccc
     5641 agttcacgcc acgcttctat gtcattgcca tgctcttcgt cgtgtttgac atcgaggcag
     5701 tgttcatcta cccctgggcg gtcatcttcg acgacctcgg gctgttcggc ttcgtcgcga
     5761 tggccgtgtt catgttcctg ctccttgacg gctacatcta tgcgtggaag aaaggggccc
     5821 tcgaatgggt ttgatcgacg atcgcttcga caagaatgtt ttcgtgacct caatggactt
     5881 cctgttcaac tggggtcgcc ggcaatccct ctggtggcta cagttcggtc tcgcctgctg
     5941 cgccatcgag atgatcgccg cgtccatgtc gcgctgggat atccccgagc gctggggcat
     6001 gctcttccga gcctcgccgc ggcaggcgga cctcatgatc gtgtccggca ccgtcaccaa
     6061 gaagatggcg ccgatcgtcc gcaccctcta tgaccagatg cccgagccga agtgggttgt
     6121 ctcgatgggc tcgtgcgcca acgtcggcgg cccgtacgac acctacgccg tggtgcaggg
     6181 tgtcgatcaa ctgatcccgg tcgatgtcta cgtgccgggc tgcccgccga cgcccgaggc
     6241 gctctactac ggcatccttc agctgcagga caagatcatt cgctacggca ccatggccag
     6301 gaagcgtggc gtcgacgccg ccgaggagca gcgccgtgcc gaccgcagtg aggccatggc
     6361 cggcgtgcgc ggctagtccc gcgggccaag gcatggcgca agcaaggagc catccgtgag
     6421 cgacggtgat atccgcgaca accagaacgt gactgacgaa ggcacgatcg gtgcaccggg
     6481 ctccgaccag ccaacccagg accagggcca gtctcccgcc tcgtacaagg acaagcgcag
     6541 cgacgaggcc gatcacgtcg aggaactcga gcaagacctc gacctcgaca cgcacgacgt
     6601 ggactggtcc cgcgaggcct attacgctga ggatctggag cgacaccccg cggtgcgtgt
     6661 gctcagtgca agtctgccgg gtgcgattgt cgatatcatc cgcttccggg acgagacaac
     6721 tatccatgtg cagccggagc ttttccgcga ggtctgccgg acgttgcgcg acgatgagaa
     6781 tgtcgccttc gacttcctga ccgacctcac tgcagtcgac atgctgcgat tgcgcaccag
     6841 cccgcgcttc gacgtggttg cgcagctcta ctccctgcgc aaccgcatgc gcgtgcgcat
     6901 caaggccggc gtcgacgacg ggcagtccgt cccctccctt gtgccactct ggaacggcgc
     6961 gaactggatg gaacgcgagg cctacgacat gttcggcatc aacttcgacg ggcacccgaa
     7021 cctcaagcgc atactgttgc ccgacgactg ggatgaaggc cacccgttgc gcaaggacta
     7081 cccggtgcgc ggctggaagg aattcccggt ctacaacaca gaacgtacgg ttccgcgcct
     7141 gcgcacgcgc tggaccggaa ggggggtctg attcccatgg catccaccac gggtcgacca
     7201 gccaacgacg acatcaggga catcgagcag tcgccgtacg gcaagcccgg cacggtgatc
     7261 gacgagacca cgccacccat cggcacgctg accatcgagg gcgagcgcac gctggacacc
     7321 ggcgagcgcg agatgaccct gaacatgggg ccgcagcatc cgtccactca tggtgtgatg
     7381 cgcgtcttgc tcaagcttga cggcgagatc atcagcgagg ccgacccggt gatcggctat
     7441 ctccaccgcg gcaccgagaa gctcggcgag atcaagcgtt acgcgcagat cactccgtgg
     7501 accgaccgca ccgactacgt cgccgcgcca tcgaacaacc tcggctacgt gctggcggtc
     7561 gagaagctct gcgggatcga ggcgccggag cgcgcgcagt actggcgcgt gatcatggcg
     7621 gagctcacgc gcatcgcctc gcacctggta tggctgggca cacatgcgct cgacatcggc
     7681 gcgctctcga tgtcgctcta ctgcttccga gagcgcgaga tcatcctcga catcttcgag
     7741 agcttctgcg gcgcacggtt gaccaccaac atgatggaga tcggcggctt ctcccgcccg
     7801 atcccggacg gactggctgg tcaagtccgc aacttcattc gtgtcttccc cggacgtcag
     7861 gcggagtacg acgccctgct gaccgcaaac cccatctgga tggcgcgcac caagggtgtc
     7921 ggcatcatct cgccggaggc ggccatctcc tactcgctca ccggcgccat gctccgcgcc
     7981 tcgggcatca actacgacgt tcgcaaggcc cagccctacc tcgtctacga tcggctcgac
     8041 ttcgaggtgc cgctcggcga gcatggcgac acctacgacc gctaccttgt ccgtatggag
     8101 gagatgcggc agtcggtccg gatcattggc caggcgctta actacgtcga acacatggac
     8161 acgccgctgg ccaacttcga gtcgccgtac gtcatcccgc cgcacaaggg cacgatgacc
     8221 accgcggagg acatgcagcg ccacttcgtc tggacgatca agggcttcaa cccaccggtc
     8281 ggcgaggtct ggcagacgat cgagcactcc aagggcgagc tcgggtacta cctcgtctcc
     8341 gacggtgggc cgatgcccta ccggttccgg atccgcacac cggacttcgt gaacctgcag
     8401 gcgctgccgc acatggccca gggcgcgctg ctcgcggatg tcgtcgccct gatcggcacc
     8461 atcgacatcg tgctcggctc cgtcgaccgc taggtcgacc ggctcgtgtc ggcaacacgc
     8521 ctaacatcgc ggtaccattg cgcagtgtgc cgttggcccg cgcaaaacgg gcgtgcggtc
     8581 gccagtattg gctggtgccg ggtgtcaggg agatagagaa gagcgttggc ggatcccggt
     8641 tccgtgccga tccccggtgg cgcttgtcaa aggctgattg ctcaagatgg accaaccgat
     8701 ctggatcacg ttcatcgtgg ccttcgtcat cctgaacgtg ctgttgatca gcatggcgta
     8761 catgacgtgg ttcgagcgca aggtgctcgc cgcgatgcag gatcggctgg gcccgactcg
     8821 caccgggtgg cacggcctcg ggcaaccaat cgccgatgcc gtcaagctgc tcggcaagga
     8881 agacctggtc cccgccggag cggacaagat cgtcttccgc ttcgcgcccg tgatcgtctt
     8941 cgtggcggcg ctgttgtcgg cggcggtcat cccgttcggc ggaacgatcg agctcttcgg
     9001 ccaggaagtc aaccttttca tcaccgacct caacgtcggc gcgctcttca tccttgcggt
     9061 cggcgccgcc ggggtctacg gcatcatcct cggcgcctgg tcgtcggcca accgctactc
     9121 ccttctgggc gggctccgct ccgccgcgca ggtgatctcc tacgagatcg tgctgggcct
     9181 ctcgctggtc ggcatcttca tcctgaccgg ctccctgagc ctcctcgaca tcctggagcg
     9241 tcaggccgaa accatcgcgc tcgggccgct cgagatcccc aactggttta tcctctccca
     9301 gccgctggcc ttcatcctct tcatcatcgc ggtcgtcgcc gagaccaacc gcgccccgtt
     9361 cgaccttccc gaggccgaga cggagctggt cgccggcttc cacaccgagt actcgggctt
     9421 ccgcttctcg ctctacttcc tcggcgagta catcaacatg atcgtggcct cgctcttcgc
     9481 ggccacactc ttcctcggcg gccccaacgg ccccggctct gacgagtact gggggctcgg
     9541 gctgctctgg ctcttcctga aggcgctcat cttcctcttc ttctacgtct ggctgcgagg
     9601 cacgatcccc cgattccgct tcgatcagct gatgggtatc gcctggaaag ttctgctgcc
     9661 gctggccctg gccaatatca tcatcacggc gttcatacgg ctcgtcgcaa acggcgaggt
     9721 cttctaaccc tgccggtcgg gaggctgccc ataccatgcg tacgcaatcg agcgatcagc
     9781 agtgagccgc atcacgttag aaccgcgata ggggaacagt ccgatgccct ggggtgtgat
     9841 ctttttctac ctgatagcga ccgtgagcat tctcagcgcc atcggcgtcg tggcagcacg
     9901 gaacccggtc cactcgtcca tcttcctggt gctctgcttc ctcaacgtgg ccggtatctt
     9961 cgtcatgctc ggcgccgagt tccttgcggt cattcaggtg atcgtctaca ccggtgccat
    10021 tctggtgctg gtcctcttcg tcctgatgct ggtcaacccg gacgagctgc cctccttcac
    10081 ggacgcacag cccctgcaac gctacctggg tctcctcctc ggtgccgtcc tcctgcttga
    10141 ggtcggcgca gcgatcatca cccgtacggc actcgaggga gtcggcgagg ccacgccgga
    10201 gaatgttgcc gctgtcggcg gcaacgtgca cgccgttggc cgcgtcctct tctccgacta
    10261 cctgctggca ttcgaggtga tctccatcgt gctgacggtg ggcatccttg gcgccatcgt
    10321 gctggctgcg cccgaacggc tcggcgagaa gctggcgacc cgccgcggca cgatcagcct
    10381 gagccacacg cgcggtacgg acgccgagct gccggtaggg ccgaggggtg aaacacccat
    10441 caagaccgat ccaaaccgcc gtcccccgcg gccggaggca ccacggcgcg tcatcaaggt
    10501 gacggacccg gacgagcgga ccaccatcgg cggcacgcgc cgctagccgg ccgctcgcag
    10561 gaaggacaga tacgagcaat catggcagag cttggcatcg agcactttct cttcttgagc
    10621 gcggccctct tcgtcatcgg catgacgggg ctcctgacgc ggcggaacct gcttgtcatc
    10681 ttcatgtgtg tcgagctgat actgacctcc gtgaacatct cactggtcgc gtttggctgg
    10741 gagacgaaca cactcaacgg tcagatattc gcgatcttcg cgatctcgat tgccgccgcc
    10801 gaggcggccg tcggactcgg catcatcctg gcgctcagcc gccggatccg ttcggtcgac
    10861 gtggccgaca tccgcgagct gcgcgactag ccggtggcat tgcctgaacg ccaccgtgcg
    10921 tatggacctg ggaacggggt aggttgagga cgcatcgatg gaagacttcc tgtatctgat
    10981 tccgctgctg ccactggtgg cgtttgcgat caatcttctg ctcggcagga actggatccg
    11041 cggcaacgcc cactgggtcg cactgccgct ggtcgccgcc agctgggtcg tggccctgct
    11101 cgccttttgg gaggtctacg acaccgagca cgcgatctcc cagcacctct tcacctggat
    11161 cccctccggc aacttcgtgg tcgacgtgaa tctctacgtc gacgcgctca cggccgtgat
    11221 gctgctggtg gtgctcacgg tcggtctgct ggttcatgtc tactctgttg gctacatgca
    11281 cggcgacccg ggcttctacc gcttcttcgc ctacctgccg ctcttcgtct tctcgatgct
    11341 catgctggtg ctggcggaca acctgctggt gctcttcgtc ttctgggaag cggtcgggct
    11401 ctgctcgttc ctcctcatcg gctactactt ccgccgccgc tcggccggca acgccgccaa
    11461 gaaggccttt atcgtcaacc gtgtcggtga cctcggtttc ggactcggcg tgatcctcac
    11521 cttctggacc ttcggcacca tctccttcta tggtgaggag ggcatcttcg agcaggccat
    11581 caaccacaca gagttcggca tcggcgaggg gacgctcgcg gcgatcggca tcctgttctt
    11641 catcggcgcg accgggaaga gcgcgcagtt cccgctgcac gtctggctgc cggacgccat
    11701 ggaaggcccc accccggtct ccgcgctcat tcacgccgcc acgatggtca ccgcaggcat
    11761 ttacatggtg gcccgcaact acacgatctt catccagtcc gaaaccgcaa tgctggtcgt
    11821 agccgtggtt ggcgcgttca ccgccttcat ggccgcgacg atcgccctca cgcagaacga
    11881 catcaagcgc gtggtggcct actccaccgt cagccagctc ggctacatgg ccttcgcgct
    11941 cggcaccggg gcgtgggtgg cggcgatctt tcacctgatg acccacgcct tcttcaaggg
    12001 cctgctcttc cttggcgcgg gctccgtaat ccatggcatg cacgaggagc aggacatccg
    12061 caacatgggt ggcctgcgca agcacatgcc ggtgaccttc tggaccttcc tgatcggctc
    12121 gctggccaac gccggcgtga tcccgctggc cggattctgg tcgaaggacg aggtcatcgt
    12181 cggcgcatgg atcagcgaat cctggttcgg cgagatcgct gcagtgatcg gcctggtggc
    12241 cgccttcctg accgcgctct acatgttccg tctcgtcttc ctcgtcttct tcggcgagga
    12301 gcgcttcgac accgagcacc tgcacccgca cgaatccggc ggctggatgg cctggccgct
    12361 catcctcctt gcgatcccgt cggtgctcat cggcttcgtc ggcttcccgc cggaggacgg
    12421 cccgttccac cacttcgtcc accacgcctt cgagttcgtc gaagtccatc acccgtcgac
    12481 aaccatgctc atcaccttcg gtgtgatctc cacggcggtc gcgctcggcg gcatcttcgt
    12541 ggcctggctg gtctacatga agggtgcgat caacccagcg tctgtcagcg agaagtacga
    12601 aggactctac gagttcctgc tcaacaagtg gtactttgac gagctgtatg accggctcat
    12661 cgtccagccg ctgcgtgcct tctcggattt cctgtggcgt gtggtcgacg aaggcatcat
    12721 cgacgccgcc gtgaacggcg tcgcggtcgg catcgcggca gtgagccagc gcgtccggca
    12781 cgtgcagacc ggcctggtgg ccaactacgc gctcgcgatc gcgctcggca tgatcttgct
    12841 gatcggagcc ttcatcgcgg cctactcgaa cctgttcaga tagagaaggg gtgatcatgg
    12901 ggttcgcccg tacgggtcgg ctccatgtcg acccctggca catcacccgg aggagcaagt
    12961 ggacttcccg ctcttaagca caattgccta tctgccactg gtcggtgcgc tcgcgatcct
    13021 cttcgcgccg agagcctcca gcgagctccc acggaccatc gcactggtcg cctcgctcgc
    13081 ggccttcgcg gtctcgctgg tgatgctctt ctcgttcgac cggaacgccg agttccagtt
    13141 cgtggagcag gtggtgtggc tggatgacct cggcgtctcc tacttcatgg gcgtcgacgg
    13201 catcgccgtc ctgctgatcg cgctgaccac cctgctctcg gtgatcgcga tctggtggtc
    13261 atgggccacg attcgggagc gaacgcgcga gtactacatc gccatgctcc tcctggagac
    13321 gggcatgctc ggcgtcttca tggcgctcga cctcttcgtc ttctacatct tctgggagct
    13381 cgtgctgatc ccgatggcac tgctgatcgg catctggggc agcaccgacc gggtgtacgc
    13441 ggcggtcaag ttcttcctct acaccctgtt cggctcgctg ctgatgctcg tcgccatcgt
    13501 cgcgacctac cagagctact tcaatgaaac cggtatccgc acgctcaacg tgctcgagct
    13561 tcaggccggc acctacggca ccaccttcca gttctgggtt ttcgccgcct tcttccttgc
    13621 ctttgcagtg aaggtgccga tgttcccgtt ccacacctgg ttgccggacg cgcatgtgca
    13681 ggcgccgacg gccgcctcgg tcatcctggc ggcggtcatg ctgaagatgg gtggctacgg
    13741 cctgctgcgc ttcaacctgc cgctcttccc ggagggcacc gatgcatggt cggtggcgat
    13801 catcgtgctc tcgatcatcg gcatcatcta cggcgccctc gtcgcgctgg tccagcccga
    13861 catgaagaa
//
LOCUS       DASYWL010000235         3526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_105424, whole genome shotgun sequence.
ACCESSION   DASYWL010000235 DASYWL010000000
VERSION     DASYWL010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105424"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..366
                     /locus_tag="VGR22_08700"
     CDS             172..366
                     /locus_tag="VGR22_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128682.1"
                     /translation="MLYPVDAEIVAPVGMSVEDEGELEGLLQMADANRDGDEQAAIER
                     LVTILDTARSQAEDGEFAAT"
     gene            581..1570
                     /locus_tag="VGR22_08705"
     CDS             581..1570
                     /locus_tag="VGR22_08705"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV2128683.1"
                     /translation="MRSASPTSGVPAADSKTDRAVPIPQPTTREFVIVHLGLLTVAML
                     WGGNFAAIKHLLETLTPVDVVVIRSVGAGVFYGIFLAATGRLIIPMTGPDARRMVFIG
                     VLGIAIMSLAMAYGLNRLNAGLASLLVSSNPIFTAIISRILLGERLTSRKVAGIVVAF
                     VGFLLVLQFGSAGGARLESDQIVGVLIVLLAPLSWAFYTVLSKPLLGRYQPKYVASYT
                     TMAGGTIFVPFLLFDGSLHDRMGELDAIDWVTVLYVSLLAITVAYLLWYWGLRVLTPS
                     QTAVYTYLVPVFGVLASWILLGEIPAIFALVGGVVIVAGVILANTSGRRSKAE"
     gene            2075..3463
                     /locus_tag="VGR22_08710"
     CDS             2075..3463
                     /locus_tag="VGR22_08710"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEV2128684.1"
                     /translation="MVAPVETNNTRSIVAAQPSPEEIYAQIEAEGVQFINLQFTDVMG
                     MVKSVTIPASIFSHVVEGGQWIDGSSIAGFTRIAESDMYLQPDLSTYAVFPWERDAFP
                     TARVICWVHNPNGDPFPGDPRGVLLRQLEKLAEMGYTYNVGPELEFFLFSKDGDRIAP
                     APHDRGSYFDFSADLASTVRKDMVSALMAMDIDVEASHHEVAIGQHEIDFEYGPAVVT
                     GDRAVSFKYILKAIAQRHGLHATFMPKPLAGVNGSGMHVHQSFGFLDPTEGKNAFVDE
                     SDPYGLSKMARHFIAGQLKHARALCGVIAPLVNSYRRLVPGFEAPVYVAWARTNRSAL
                     IRVPAIRQGNLNATRIELRCPDPSCNPYLAFTAMLAAGMDGIRNELPLEDPVEENLYH
                     FTDEDLRRRNIPTLPATLGEAIQELEQSVVIREALGDHIFERLIEAQRADWDAFRTYV
                     SDWERERYLETY"
BASE COUNT          615 a         1149 c         1125 g          637 t
ORIGIN      
        1 ccggaatggg tggctcaatg cgcccccacg aggatgtcca ggcaaatcag gggctggaga
       61 acatggacca ttggaaacat atcagtgcca acgtgaccac tggccgagct gccgacgaga
      121 agctgtgcga tctgatcggc aagcatgtgg cccggtatct caactcctcc catgctgtac
      181 cccgtcgatg cggagatcgt cgcccctgtc ggcatgagtg tcgaggacga aggtgagttg
      241 gagggcctgc tgcagatggc ggacgccaat cgcgatggtg acgagcaggc cgcgattgag
      301 cggctggtta ccattctcga cactgcacgt agccaggccg aggacgggga gttcgccgcg
      361 acctaatcgc agagcagggt ctcggggtga gggcgttcac cgggaaggta tggtgccggg
      421 gccgggtggc gatcggttgg gaagagccgc gatgcagccg ctgagaagag cttgtccccg
      481 agcggtggtc aggcgccagg gtgattggtg acggcaggcc gctgagggct gcccagccga
      541 gtgtatatcc agctgctgcg ctgagcagta cactggttcc atgagatctg cctcgcccac
      601 ctccggcgtc cccgccgccg actcgaagac cgatcgtgcc gttcccatcc cgcagccgac
      661 cacgcgcgag ttcgtcatcg tccacctcgg gctgctcact gtcgcgatgc tctggggcgg
      721 caacttcgcc gcgatcaagc acctgctgga aacgctcacc ccggtcgatg tcgtggtcat
      781 ccgcagcgtc ggcgcgggcg tcttctacgg catcttcctc gccgcgactg gacggctgat
      841 cattccgatg accggtccgg atgcgcgccg gatggtattc atcggcgtcc tcggcatcgc
      901 gatcatgagc ctggcgatgg cctatggcct gaaccggctc aacgccggcc ttgccagcct
      961 gctggtaagc agcaacccga tcttcacggc gatcatctct cgcatcctgc tcggcgagcg
     1021 gctcacgagc cgcaaggtcg ccggcatcgt cgtcgccttc gtcgggttcc tgctggtgct
     1081 gcagttcggg tcggccggcg gagcccggct ggagtccgac cagatcgtcg gcgtgctgat
     1141 cgtgctgctg gcgccgctct catgggcgtt ctacaccgtc ctgagcaagc cgctgctcgg
     1201 acgataccag ccgaagtacg tggccagcta cacgacgatg gccggtggga ctatcttcgt
     1261 tccgttcctg ctcttcgacg gctccctgca tgatcggatg ggcgagctgg atgcgattga
     1321 ctgggtgacg gtgctctacg tctccctgct ggcgatcacg gtggcgtacc tgctctggta
     1381 ttgggggctg cgtgtgctga cgccgagcca gacggcggtc tacacctacc tcgtgccggt
     1441 cttcggtgtg ctggcgtcat ggatcctgct gggggagatt ccggcgatct tcgcactggt
     1501 cggaggcgtg gtgatcgtcg ccggggtgat tcttgccaac accagcggcc gtcgctcaaa
     1561 ggcggagtag gggaagccga gctgtagacg cgggtgtcga tgcccggatg gctggtagtt
     1621 gttgccaacg tgcggatgga agaccccagc cagacaggtg tcatcctgag cccttcggct
     1681 tcgctcagga taaactccgc gaaggatccc cggcgcgcag cgccggtcca gtcttttcgt
     1741 ttgtcgtcga tatcctgatc cgcatcatgg cgaatgccga aagaccgccc ttcgggcgga
     1801 agatccttga cacgtttcgg cactggcgcg cctcaacgtc tcaggatgac agagctttga
     1861 cgtgtccctg cccaagagca gggacacccc gacggcgcac cggcctgcgc cgccgccgtt
     1921 gtgcaacatg cacaaatccc tgtggtcgat ttcaccacca tgcacgatgc tccattccgg
     1981 aagcgacagt aggcttgagg ccaatgggtg cgcaggacca tgcaaccaga ggcatcgtca
     2041 ggtcacaccg ccggcaggca gggggaacag tactgtggta gctccggtcg aaacgaacaa
     2101 cacgagatcc atcgtcgcag cgcagccgtc acccgaggag atctacgccc agatcgaggc
     2161 cgagggcgtg caattcatca acctgcagtt caccgatgtc atgggcatgg tcaagagcgt
     2221 caccatcccg gcatcgattt tctcgcacgt cgtcgagggc gggcagtgga tcgacggttc
     2281 gtccatcgcc ggcttcaccc gcatcgccga gtccgacatg tacctgcagc cggacctctc
     2341 cacctacgcg gtctttcctt gggagcggga cgcgtttcca accgcgcgcg tgatctgctg
     2401 ggtacacaac cccaacggcg accccttccc cggcgacccg cgcggcgtac tcctgcggca
     2461 gctcgagaag ctcgccgaga tggggtacac ctacaacgtc gggccggagc tggagttctt
     2521 cctcttctcg aaggacggcg acaggatcgc acccgcgcca cacgaccgtg gcagctactt
     2581 cgacttctcc gccgacctcg cctccaccgt ccgtaaggac atggtcagcg cgctgatggc
     2641 gatggatatc gacgtcgagg ccagccacca cgaggtcgcg atcggccagc acgagatcga
     2701 cttcgagtac ggcccggcgg tggtgaccgg cgaccgtgcg gtctccttca agtacatcct
     2761 caaggccatc gcccagcgac acggcctaca cgctaccttc atgcccaaac cgctggccgg
     2821 cgtgaacggc tctggcatgc acgttcacca gagcttcggg ttccttgacc cgaccgaagg
     2881 caagaacgcc tttgtggacg agagtgatcc ctacggtctc tcgaagatgg cgcgccactt
     2941 catcgccggt cagctcaagc atgctcgcgc gctgtgtggc gtgatcgcac cgctggtgaa
     3001 cagctaccgc cgcctggtgc cgggctttga ggcgccggtc tacgtcgcct gggcgcgcac
     3061 caaccgctcg gcgctgatcc gcgtgccggc gatccgccaa ggcaatctca acgcgacgcg
     3121 catcgagctg cgctgcccgg atccgtcctg caacccatat ctcgccttta ccgcgatgct
     3181 ggccgccggc atggacggca tccggaacga gctgccgctg gaggacccgg tggaggagaa
     3241 cctctaccac ttcaccgacg aggatctcag gcgtcgcaac atcccgacgc tgccggcgac
     3301 gctcggcgag gcgatccagg agctcgagca gagcgtcgtc atccgcgagg cgctgggcga
     3361 ccacatcttc gagcggttga tcgaggcgca gcgagccgac tgggacgcgt tccgcaccta
     3421 tgtctctgac tgggagcgcg agcgttacct cgagacctac taggaacgat cgcgacacca
     3481 gtcgcaccac aggaaccccc ggtcgccacc acggcgaccg ggggtt
//
LOCUS       DASYWL010000236         6444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_86283, whole genome shotgun sequence.
ACCESSION   DASYWL010000236 DASYWL010000000
VERSION     DASYWL010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6444
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86283"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VGR22_08715"
     CDS             complement(<1..384)
                     /locus_tag="VGR22_08715"
                     /inference="COORDINATES: protein motif:HMM:NF028174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase dimerization domain-containing
                     protein"
                     /protein_id="HEV2128685.1"
                     /translation="MFPGTEGLSPRAALVQLITASWVSHTVRAMAALSLADHLADGPR
                     TSAELADATGTDASTLARFLRTLAALGLVAHDAEDRAGLTPLGELLRANVPGSLRPYA
                     LAIAAPHVERAWHELPQALRTGEPSF"
     gene            complement(530..679)
                     /locus_tag="VGR22_08720"
     CDS             complement(530..679)
                     /locus_tag="VGR22_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128686.1"
                     /translation="MTFTPQITTDTLTVPAELVPHEEGAEPIRATIWLSLTQNDLIRR
                     GVGRG"
     gene            complement(690..1082)
                     /locus_tag="VGR22_08725"
     CDS             complement(690..1082)
                     /locus_tag="VGR22_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128687.1"
                     /translation="MAKHEQCIGPTKSGQPCKGTPLPSKAYCFAHDPDLDNQRQSWVK
                     QAGKAKSNKARMKKQLGDGFLDMKDIDGMLCLTLKGVLAGKIEPGVANAAATVAKTIQ
                     GIRTTTDLQERLEAIEEALDRADRRRYA"
     gene            complement(1101..1370)
                     /locus_tag="VGR22_08730"
     CDS             complement(1101..1370)
                     /locus_tag="VGR22_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128688.1"
                     /translation="MSRLLESQIARIEERAQRTLRDTCTRCWGRGAIILCVPNEEDAH
                     LPEYNSTACPGCGMQLRFFRRVIGISEEEMFPEMVESDNNDESVE"
     gene            complement(1367..1585)
                     /locus_tag="VGR22_08735"
     CDS             complement(1367..1585)
                     /locus_tag="VGR22_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128689.1"
                     /translation="MTNKIERQLSEIEKRLHLGGEGDWCAACVHEQRFQILYDDEPEA
                     CCPECGRVLGPLEGTRVIRIVLREDGPQ"
     gene            complement(1582..1764)
                     /locus_tag="VGR22_08740"
     CDS             complement(1582..1764)
                     /locus_tag="VGR22_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128690.1"
                     /translation="MSQEQRNRIVDALEKLLDAHMHRQAPRYRVGDRPSKSCVGGHGL
                     EYRDVGAITLREGRAR"
     gene            complement(1869..2003)
                     /locus_tag="VGR22_08745"
     CDS             complement(1869..2003)
                     /locus_tag="VGR22_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128691.1"
                     /translation="MTASNQSQAPQPNDQDSELLAYPLQDGWLLTVIGLGGEADIWLT
                     "
     gene            complement(2000..2293)
                     /locus_tag="VGR22_08750"
     CDS             complement(2000..2293)
                     /locus_tag="VGR22_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128692.1"
                     /translation="MSTISTIKNLQAKTGYTSFNVSVIHCKAKLPAAVWRSLSVAERP
                     YVCQDITIVAVDDGGETAAVMLARHYDTLIERIRQLETWIGEFIEESDQEEAS"
     gene            complement(2290..2694)
                     /locus_tag="VGR22_08755"
     CDS             complement(2290..2694)
                     /locus_tag="VGR22_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128693.1"
                     /translation="MLEQLHQAKAARNAKLRTLAVGADLIPRSDRNDPHLINVDPWEL
                     VSRAMNAEVRATGLARLSVTVERLPEALRARAEEIGIELARAGMLEIGRWATDVQLAY
                     VRVDGISEEVQAAMFHAWGVAIEHLSEGDLPA"
     gene            complement(2697..3602)
                     /locus_tag="VGR22_08760"
     CDS             complement(2697..3602)
                     /locus_tag="VGR22_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128694.1"
                     /translation="MIHTIRDDVVNAEGETVAEGVEDKRAQFIEEEMSQLLKTAQRQG
                     ATISEMIRRAWDSPDVLRTITKTSPSKATDPHISIIGHITKSELAKHITETELANGLA
                     NRFAWFRVHRTRLLPDPRPIALEVVQELGGRIHEALVYGRSVDKVSRDPEASDLWKIA
                     YSGLEEDRPGLAGAIIARSSPIVCRLSLIYALLDHSPVIRAPHLEAAVAVWDYAQKPV
                     ESIFGDMTGDDVADRIIDVLTTEGQQTRTQLHEAFGRNVSAARIGQALTMLHHAGKIR
                     AFKQKPKGAGRPSTVYRLAESGVES"
     gene            complement(3606..4040)
                     /locus_tag="VGR22_08765"
     CDS             complement(3606..4040)
                     /locus_tag="VGR22_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128695.1"
                     /translation="MSAVPLNDEFVRRFGDYKPRKQAAEDVEYEEIGPAVSRLNVKPE
                     MHPAAYYGLAGDVVRAITPTTEADPVAVLVTFLAEFGNAVGRRPHMMIGESRHGANLF
                     VVLTGETARGRKDDSTKGVKRFMQEADQTWTFDHLMGGLSSG"
     gene            complement(4090..4662)
                     /locus_tag="VGR22_08770"
     CDS             complement(4090..4662)
                     /locus_tag="VGR22_08770"
                     /inference="COORDINATES: protein motif:HMM:NF013930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HEV2128696.1"
                     /translation="MNVPTSYHARGALAPQCIHDLRFLYTEYLELESRHHKEVREEQR
                     RAGIDTADVDLQIDALDAELVRRAALYLQSDELPPWPRLDDERYAQLLEDARELKAIW
                     PLADFLTRVVGLELHGRGPGLAGDCPFHQSKSRASFKVSTEKNVWHCFGCNKGGDLFR
                     FVGGYYDVTSFPDQVRKVEEVTATMWSRRA"
     gene            complement(4668..4928)
                     /locus_tag="VGR22_08775"
     CDS             complement(4668..4928)
                     /locus_tag="VGR22_08775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HEV2128697.1"
                     /translation="MTTQELEQRIAFRIEEAARVIGASRSKMYELVASGEIPSFRVGR
                     AVRVSKKAPEEWIAHQESHTAAEAKRKRRARNTRRFLKGRPP"
     gene            5636..>6444
                     /locus_tag="VGR22_08780"
     CDS             5636..>6444
                     /locus_tag="VGR22_08780"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV2128698.1"
                     /translation="MVQQRGIYHPNSPAPASQSSHVLLLLVMLLLATVPVIAVAEPSP
                     PARASTVDVIATDTLYLRDCPSLTCRIAGNAALGTELEKTGEQANGFLPVCSFGTDGW
                     AYELFLTHRSDRSLVRKGVPGCDRIALIFNAGIGKQPSESILAALAETQVPVTVFAMG
                     WWAESYPDYLRRLDRDTRAVIGSHGDTQTFLTHAANAQVVAEVHNSATRIKSVLGYPV
                     ARYYTAYASDTDARVERIIAEEGYLPIGWTVSAGDYRSDNSAEEVRTKVLDG"
BASE COUNT         1109 a         2092 c         2019 g         1224 t
ORIGIN      
        1 aaaggacggc tcaccggtac gcagcgcctg cggcagctca tgccaagctc gctcgacgtg
       61 gggagcggca atggcgagcg cgtagggacg cagcgaaccg ggcacattgg cacggagtag
      121 ctcgccgagg ggagtcagtc ccgccctatc ctccgcgtcg tgagcgacca agccgagcgc
      181 agccagggtc cgtagaaaac gagcgagggt cgaggcgtcc gtgcccgtcg cgtccgccag
      241 ctcagcggag gtacgcgggc cgtccgcgag gtgatcggca aggctgaggg cggccatggc
      301 acggacagtg tgggagaccc aggaggcggt aatcagctgc acgagcgcag cacggggcga
      361 gagcccctcg gtacctggaa acacgtcacc ctctttcggc aagtggaggc gcgagggctg
      421 cggggtcggt ggggtcccat gggaatgctg tcccccagca tacacctcat ccgcccagaa
      481 agatatcggc ccttggacgg gtgcagcctc tctgatcggt tccgcatgtt catcctcgac
      541 caacgccgcg ccggatcagg tcgttctggg tgaggctgag ccagatggtg gcccggatcg
      601 gctcggcgcc ctcctcgtgc ggaaccagct cggccgggac ggtcagcgtg tcggtcgtga
      661 tctgcggcgt gaaggtcatg atgatgctcc tatgcgtagc gccgacggtc ggcgcggtcg
      721 agtgcttcct caatggcttc cagacgctcc tgcagatcgg tcgtggtccg gatgccctgg
      781 atcgtcttgg ccaccgtcgc ggccgcgttg gcgacaccgg gctcgatctt gccggccagc
      841 acgcccttga gggtgaggca gagcatcccg tcgatatcct tcatgtcgag gaacccgtcg
      901 cccagctgct tcttcatccg ggccttgttg ctcttcgcct tgccggcctg cttcacccac
      961 gactggcgct ggttgtcgag gtcaggatcg tgggcgaagc agtacgcttt gctgggcagc
     1021 ggcgtgccct tgcacggctg tccggatttg gtcggtccta tgcattgctc gtgcttcgcc
     1081 acgtatcccg cctttctggc tcactcgacc gattcgtcgt tgttgtcgga ctcgaccatc
     1141 tccgggaaca tctcttcttc actgatgccg atgacgcgcc gaaagaagcg gagctgcatg
     1201 ccgcagccgg gacaggctgt tgagttgtac tcgggcagat gcgcatcctc ttcgttcggg
     1261 acgcaaagga tgatcgcgcc cctgccccaa catcgcgtgc atgtgtccct cagggtccgc
     1321 tgcgctcgct cctcgattct ggcgatctgg ctctcgagca gtcggctcat tgcgggccgt
     1381 cctctcgcag cacgatgcga atcacccgcg tcccctccaa cgggccgagc acgcggccac
     1441 actcgggaca gcatgcctcc ggctcgtcat cgtagaggat ctggaagcgc tgctcatgga
     1501 cacaggcagc gcaccagtcg ccctcgccgc cgaggtggag gcgcttctcg atctcgctga
     1561 gctgccgctc aatcttgttg gtcatcgtgc tcgcccctct cgtagcgtga tcgccccgac
     1621 gtcgcgatac tcgaggccgt gtccgccgac acagctcttg ctcggccggt ccccgacgcg
     1681 gtagcgtggc gcctgccggt gcatgtgcgc atcgagcagc ttctcgagcg cgtcgacaat
     1741 gcggttgcgc tgctcctggc tcatggtgcg aagatcgggc agagcgccgg cgtgacacca
     1801 agcgcgtcca gctcgaagag cagccggtca ggaacgcgga gcccgaaccg tacctccggc
     1861 gctcccttct aggtcagcca gatatccgcc tcgccgccga gcccgatcac cgtgagcagc
     1921 cagccatcct ggagcgggta ggcgagcaac tcgctgtcct ggtcgttcgg ttgtggggcc
     1981 tgactctggt tgctggcggt cacgaggctt cctcctggtc cgattcctcg atgaactccc
     2041 cgatccaggt ctccagctgc cggatgcgct caatgagtgt gtcgtaatgc cgagccagca
     2101 tcacggcggc ggtttcaccg ccgtcatcga cggcaacaat ggtgatgtcc tggcagacgt
     2161 agggccgctc ggccaccgag agcgagcgcc acaccgcagc cggaagcttc gccttgcaat
     2221 ggatgacgga gacgttgaac gacgtatagc cggtcttcgc ctgcaggttc ttgatcgttg
     2281 agatggtgct catgccggta gatctccctc gctgagatgt tcaatcgcga caccccatgc
     2341 atggaacatc gccgcttgca cttcctcgct gatgccgtcc acccgcacat acgccagctg
     2401 gacatcggtc gcccatcggc cgatttcgag catgccggcc cgcgccagct cgatgccgat
     2461 ctcctccgcc cgcgcccgaa gcgcctcggg taggcgctcg accgtgactg agaggcgtgc
     2521 gaggccggta gcccgtacct cggcattcat cgcccgggaa acaagctccc aagggtcgac
     2581 attgatcagg tgcgggtcgt tgcggtccga gcgcgggatc agatccgcgc cgacggccaa
     2641 ggtgcggagc ttggcgttac gtgccgcctt cgcctggtgg agctgctcga gcacgctcac
     2701 gactccaccc cgctttccgc caggcggtac accgtgctgg gccggcctgc ccctttcggc
     2761 ttctgcttga atgcgcggat cttccctgcg tggtgcagca tggtgagcgc ctgcccgatc
     2821 cgcgccgcgg acacattgcg accgaacgct tcatgcaact gcgtgcgcgt ctgctggccc
     2881 tccgtggtga gcacgtcgat gatccggtcg gcgacgtcat ccccggtcat gtcgccgaag
     2941 atgctctcga cgggcttctg cgcgtaatcc cacacagcga cggcagcctc gaggtgtggg
     3001 gcacggatca ccggcgagtg atcgaggagc gcatagatca ggctgagccg acagacgatg
     3061 ggggaagagc gggcaatgat ggcaccggcc agcccgggcc ggtcttcctc gagaccgctg
     3121 tatgcaatct tccagaggtc ggaagcctcg ggatcgcggc tcaccttgtc gaccgaacgc
     3181 ccgtagacaa gcgcctcgtg gatacgccca ccgagctcct gaacgacttc cagagctatc
     3241 gggcgaggat ccggcaggag ccgggtacga tgcacacgga accatgcgaa gcgattggcc
     3301 agcccgttgg cgagctctgt ctcggtgatg tgcttcgcca gctcgctctt ggtgatgtgg
     3361 ccgatgatgg agatgtgcgg atcggtcgcc ttgctcgggc tcgtcttcgt gatggtccgg
     3421 agaacatcgg gcgagtccca tgcgcggcgg atcatctcgg agattgtggc tccctgccgc
     3481 tgagccgttt tcagcagctg cgacatctcc tcttcgataa actgcgcccg cttgtcctcc
     3541 acgccctcag caaccgtttc gccttcggcg ttcacgacat cgtcgcgaat ggtgtgaatc
     3601 atgccctatc cagaggacag cccgcccatg aggtgatcga acgtccacgt ctgatcagct
     3661 tcctgcatga accgtttgac gcccttggtt gagtcgtcct tgcgcccacg cgcagtctca
     3721 ccggtgagga caacgaagag gttcgcacca tggcgagact cgccgatcat catgtgcgga
     3781 cgccggccca cggcatttcc gaactcggcc aggaaggtca ccagcacggc gaccggatcc
     3841 gcttcggtgg tcggcgtgat cgcccgcaca acgtcgccgg cgagtccgta atatgccgcg
     3901 ggatgcatct ccggcttgac gtttaaccgg ctgaccgccg gcccgatctc ctcgtactcg
     3961 acatcctcgg cagcctgctt gcggggcttg tagtcgccga aacgtcgcac gaactcatca
     4021 ttcagcggca cagcgctcat cgcagcaccg cctcgagctc gcggcgcatc tcgtcctgga
     4081 cagcagtgat caggctcgac gactccacat cgtggcggtg acctcttcga ctttccggac
     4141 ctgatcagga aacgacgtga cgtcgtagta cccgccgacg aatcgaaaga gatcgccgcc
     4201 cttgttgcag ccgaagcagt gccagacgtt cttctcggtc gagaccttga agctcgcccg
     4261 actcttgctc tggtggaacg ggcagtcgcc ggcaagccca gggccacgcc catggagctc
     4321 caacccgaca actcgtgtga ggaagtcggc gagcggccaa atagccttga gctcgcgcgc
     4381 atcctcgagc agctgcgcgt atcgctcatc gtccagccgc ggccatggcg gcaactcgtc
     4441 agactggagg tagagtgcgg cgcggcgcac gagctcggcg tcgagcgcat cgatctgcaa
     4501 atcgacgtcg gcggtgtcga tgcccgcccg gcgctgctcc tcgcggacct ctttgtgatg
     4561 ccgcgattcg agctcgaggt actcggtgta caggaatcgg agatcgtgga tacactgagg
     4621 cgcgagggca cctcgtgcat ggtatgatgt gggtacattc atggccgtca cggtgggcgt
     4681 cctttcagaa agcgtcgggt gttgcgcgcc cggcgctttc gctttgcctc tgccgcggta
     4741 tgcgactcct ggtgtgcaat ccattcctcc ggtgccttct tgctcacacg aacggcccgc
     4801 cccacacgga atgacgggat ctcgccgcta gcgactagct cgtacatctt ggagcggctg
     4861 gcaccgatga cccgcgccgc ctcttcaatg cgaaacgcga tgcgctgctc gagctcctgg
     4921 gtggtcatgt gtctcacctc gatacgtgac aacgaacgac agacgagcgg tactgattgg
     4981 cctcctttcc gacaacgcaa aaagccgggc tcactcggtg ccggtatcac agcaggggat
     5041 gtccatggct tgacactggt tccaagtggg cccggctctt cacgcgctca agcgtgggag
     5101 tggtgtcctc actggctatt tgaattcttg atttgcaatc tatgagtgcc gtcacagatg
     5161 cagcatcccc cactaggcga cctggcgggg gaccaccttg atcttcgcat ccgaacagca
     5221 agaagtcaag gggaaacgca acgtacactg tggacgcgtt gccaccggcc gaattacttc
     5281 gtatttgttc gttttcttcg tacaggcagg ggcggcgaca gtttcgccat gaaaccgggc
     5341 agaacggcga attagttcgt ttagctcgtt tctttcgtag cgggactcca aagagaccgc
     5401 cgcacgccac gggagctaac agtggtacaa ccacagtaat gccgagttgg tgtaactgtg
     5461 agggaaacca gctaacgcag tggaatacgt cgcgtcgcac gttggtcggc actgtcgtag
     5521 tttcgggaga tcatccagca gagagggaat gcaaggaaga gtaccatcgc gccggatcgg
     5581 ccccggacgg ctcgtcgcga cccccatcgg tgtgtacgta cgctataatc cgcgtatggt
     5641 tcaacagcgc ggcatctacc acccgaacag ccccgccccg gcatcgcagt ccagccacgt
     5701 actcctgctg ctggtgatgc tgctgctggc gacggtgccg gtcatcgcgg tggcggaacc
     5761 ctccccgcca gccagagcca gcacggtgga cgtgatcgcc accgacacgc tctacctccg
     5821 cgactgcccg tcgctgacct gccggatcgc cggcaatgca gcgctgggta ctgagttgga
     5881 gaagaccggc gagcaggcaa acggcttcct gccggtatgc agtttcggaa ccgacggttg
     5941 ggcctacgag ctcttcctca cgcatcggtc cgaccgctcc ttagtacgga agggcgtgcc
     6001 cggctgcgac cgcatagcgc tgatcttcaa cgccgggatt gggaagcagc catccgagtc
     6061 gatcctggcg gcgctcgccg aaacgcaggt gccggtgacg gtcttcgcca tgggctggtg
     6121 ggcggaatcc tacccggact acctgcgccg gctcgatcga gatacccgcg cggtcatcgg
     6181 atctcacggt gacacacaga ccttcctcac acacgccgcc aacgcgcagg tcgtcgccga
     6241 ggttcacaac agcgccactc gcatcaagag cgtgctgggt taccccgtcg ctcgctacta
     6301 caccgcctac gcctccgata ccgatgcgcg tgtggagagg atcatcgctg aagaggggta
     6361 tctgccgatc ggctggacgg tctccgctgg cgactaccgt tcggacaact ccgcagagga
     6421 ggtccggacc aaggtgctcg acgg
//
LOCUS       DASYWL010000237        13036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_142559, whole genome shotgun sequence.
ACCESSION   DASYWL010000237 DASYWL010000000
VERSION     DASYWL010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 13036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13036
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142559"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VGR22_08785"
     CDS             complement(<1..192)
                     /locus_tag="VGR22_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2128699.1"
                     /translation="MARVETRGLVKDFGEGAPAVNGIDLRTEDGEFVVLLGPSGCGKT
                     TLLRMIGGLEKPTSGEILIG"
     gene            complement(313..1656)
                     /locus_tag="VGR22_08790"
     CDS             complement(313..1656)
                     /locus_tag="VGR22_08790"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV2128700.1"
                     /translation="MATYLATRATGTGAATLVQSTPSVDISGTSLSILQWSHFVPAFD
                     EWFPQFLQEWADANGVTATVDMINTADVPATFAAEISAEQGHDIVEHIASLPQYEESV
                     LDLTELVQEAERRHGPQLEMAKRNSWNPTTDKYYGFCHGYAPDPANYRKSLWETVGLE
                     NGPSTYDELLDGGTQIWNEQGVQMGIGMSQEIDSNMAAQAMLWAFGAAVQDENENIII
                     NSPESVEAVTYMKTLFESCMTPEVFGWNAASNNQLLVAGQASYILNSISAYRTAQKQQ
                     PEVAEDIFFSTPLVGPGGEDRALAHGHAVFTAMIPTYSQNQDTAKEFLLHLVANYEAA
                     SRASELYNFPAFPETFPSLVEDGGPLDDDPSGSVPPDKLAPLKTAEEWTVNLGWPGPA
                     NAMIGEAFNTFILSDMMAQAARGDMTPEEAVADAEAKLEEIAQRWRDEGLMGGGQ"
     gene            complement(1870..2481)
                     /locus_tag="VGR22_08795"
     CDS             complement(1870..2481)
                     /locus_tag="VGR22_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128701.1"
                     /translation="MDASLLITLVSLILALGVVVKVQSAPPGTPWSREAIWHSIRSGF
                     RETEYPWPRPASYFTENIPKTWPIRSFLLVANEELAAMRRTLQSAQAVHLPADIIRSY
                     DGNMRQAGRLLNRNADRVVEAARAGATSPQLEEALIRKQHSLERLVDALQEARGSLAA
                     LIVTGLEDQRDLDQVAHSLQAWSEALREVSADTAHADLHAIRD"
     gene            complement(2503..3573)
                     /locus_tag="VGR22_08800"
     CDS             complement(2503..3573)
                     /locus_tag="VGR22_08800"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEV2128702.1"
                     /translation="MPHLNRRNLVGAALAAAIVPIASAGQSQQPGPTVWPVPDWPRTN
                     SAEMNVDPALPELLANAGAATPSVTGVVVTRAGRIVADYWADGWGPDDPIDIRSCTKS
                     VVGALVGRARHDGLLPDLTVTIGELIPERIPEGADPAVANITLWSLLTMTSGLAWTWQ
                     DDYPRLEAAADPVALTLGLPIAAPQGTAYVYNSGDSHIIGLMVAAAARMPLEEYAEEA
                     LFAPLGITMHGWRRTPQGEVIGGYGLQIVPEDMARLGYLYLRDGAWNGEQLIAPEYIE
                     QSIIVQSSGDPTGGTPYGYQWWVTTTWTGYDAFYALGYGGQYIWVVPALELVIAIAVG
                     DISVPLYPPRPIIEQTIIPLMY"
     gene            3857..6445
                     /locus_tag="VGR22_08805"
     CDS             3857..6445
                     /locus_tag="VGR22_08805"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2128703.1"
                     /translation="MYRLTPQLLRTSFVGRERDARAIADLFARGDVRIVTLTGPGGVG
                     KTRLAFHVLNRLESALPDGVVIVDLATITIPDLVLPAIAHEFGIMESGAESIMARLVR
                     VIGQRRTLLLVDNFEQVAEAGMVISRLASDCPNVAFLVTSRMPLHIAGEHEYTVSPLE
                     TPPVRGNSPNGVMLDGVPTEEYPAVRLFIERAQAAHRAFAPTPEMVRTVGEITALLDG
                     LPLAIELAAARTKLFSLQALLNRLDDRMGLLTGGPRDLPDRLQTMRNAIAWSYDILSP
                     EEKAVFRRVSIFPNSFSLEAAEAVVAGPLAEQERAFVEEYDGSPSDLEPIGNVLGHLE
                     SLADKSLLQAVTGLHEDVRFRMLLTIQEFGREQLRAERELLLMRMRLLVHSYEHLANL
                     DELLFGPEQRIWLDRLDLEQGNIRGALQFALEYPREFGVQGVHLASSVWRYWLVRGHV
                     AEGARWLEQALACRDTVEIPVLIEAEAVNRLGNLLLDLGKHEEAERHYRESLALYRSA
                     DHRDGVADELNNLGLVQLLQGRIERARSSLEESLALRRDGGDPRALPNTLSNLGDLAI
                     EERNYDRAESLHMEALSIRREIGNLRGMALSCCSLGQVAFFWKEFDRARRWFDEGLEY
                     QTQVDDSYSLAMLMLGLGRLDLATGRPVQAMKRLGRALEVSQRMGSRRMVLSVIDTIA
                     TSAVSYGFDRDAIRLFGTTQGVRERENLHHPQRECAATDRVLAMLTQRLGESVVSREH
                     TIGRRQWLNQAVQEAAALIVQIQERLETGEVGTAGVNGVFDPAAATRKARAFGLTRRE
                     RQVLALLVRGASDKEIAEALSIAPRTAMTHVSNVLAKLGVNRRTAAASYAIREGLVDP
                     GATREG"
     gene            6732..9632
                     /locus_tag="VGR22_08810"
     CDS             6732..9632
                     /locus_tag="VGR22_08810"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEV2128704.1"
                     /translation="MSDLHTFFGPNAGYVLELYDRYLADPGSVDAESRAFFESFTPPT
                     EDGAQPTPGVATAVAGSTVDLLKVAAAVNLATGIREYGHLAVPLDPLGSEPPGAPELD
                     PATYGITEDDLKSTPAEIVGGEAADGAANAFEAIERLRRRYTTRIGFDFDHVQESDER
                     IWLNDAVESGRFEVEPDEASQRKLLERLTQVEVFERFLHQTYLGQKRFSIEGNDILVP
                     MLDQITDDASAAGTRQVFLGMAHRGRLNVMTHILSKPYGAIIAAFEGGKRRAAASTES
                     ADDVRTGDVKYHLGARLARNPETGERVKMPVVLAPNPSHLEAVNPIVLGMTRAGQDDR
                     TTPGRSEQDTSCSLSILLHGDAAFPGQGVVAESLNLSSLAGYSVGGTIHIIVNNQIGY
                     TTDWRDARSTLYAGDLAKGFEIPVVHVNADDPLACLQAASMAMAYRRQFGKDFLIDLV
                     GYRRWGHNEGDEPAFTQPVLYQKISKHPTVRAIWADRLAEKGVLTEDDAAAMERRMLD
                     RLAKIRRGITEGQDTYDEQVELTSGPREEVETALSEDRLKALDAGVHKLPEDFTPNSK
                     LKRLWERRAQIVQTGEGKIDWAHAEALAFAALLADGVPIRLTGQDAQRGTFSQRHIVL
                     HDAKTGEAYAPLAQLPEAKASFAVYNSPLSENAAMGFEYGYSVQAPEALVLWEAQFGD
                     FVNSAQVVIDQFLSSARSKWGQEPTLVLLLPHGYEGQGPEHSSARLERFLQLAAGDNM
                     RVANVTSAAQYFHLLRRQAARLDRDPRPLVLMTPKSLLRNPMASSSPEQFTEGTFLPV
                     IDDAKAQDRKDRVTRLVLCTGKVAIDLEASDQRDETEHVAIVRVEQLAPFQSSILRDV
                     IDSYPNLEEIVWLQEEPKNMGAWSFMVQRLRDLIEDAYPVRYIGRTERAAPAEGSLEQ
                     HNEEQAAIVAGAFEPQRAAGGAATNGAAPSGRKRDSKKGARDRAAVAASPAE"
     gene            9720..11036
                     /gene="odhB"
                     /locus_tag="VGR22_08815"
     CDS             9720..11036
                     /gene="odhB"
                     /locus_tag="VGR22_08815"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEV2128705.1"
                     /translation="MAIEIKVPPLGESLVDAVVGQWLKSEGDAISRGETILELETDKV
                     NLDVTAEDDGVLGAIQKEEGDTVTVGEVLGVIEAGGGNGAGAPAEAEAEEPAEVEAAS
                     TAPATEPQPEPAPAAAATEEAPEPSAAEDGAGRAAPAVRRMAEEHAIDLAKVSGTGAG
                     GRITRDDIVAYLASQRGQGGAAAPVAAPPQPETAQPTPAPAPAEQREDEERIRLSRRR
                     QTIAKRLVEAQQTAAMLTTFNEVDMSAVMDLRKRRKESFKEQHGVNLGFMSFFTKAAV
                     GALKAFPEVNSELQGDELILKKHYDIGIAVGVEEGLVVPVVRNADRRSFADIEREIIE
                     LAGKARENKLAISELQGGTFTITNGGVFGSLLSTPILNTPQVGILGMHTIQQRPVAVD
                     GEVVIRPMMYVALSYDHRIVDGQQAVQFLVKIKQMVEDPETLLLEG"
     gene            complement(11177..12382)
                     /locus_tag="VGR22_08820"
     CDS             complement(11177..12382)
                     /locus_tag="VGR22_08820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2128706.1"
                     /translation="MASNPALIEIRTVSSEEYLEAGRPIASYAFGPSPQQTDMERARQ
                     NLPYYERTTNLIAFVAGGAQASMVSHEMTQNVRGMVVPMGGAGGVASMPVGRRQGIVR
                     RMFERVFELYHDAEMPSATLYPFRDSFYERLGYASFPKPRFLTLRPEALVPLVRHDKP
                     GTCEQLAMKDGFDEWRAFLERFQRHTHGFALAHVTKARQARVLNESWVALARYEGEVV
                     GAMTFKITGYGEKLIADTFYTVNSIGRYLLLDWIGRHTDQVREAVIELRPDEYPEVWF
                     HDLGEATRTDAERAWPGPMGRVVDVEKLSGIGAGQGEITLEIRDLICPWNNGIFTLRA
                     ESGVLAVEATDAKPAGHLTIQGLSALVFTGHDPAGFCFRGWGDPDTETQATLRALFPP
                     AIPDIHEKF"
     gene            complement(12530..>13036)
                     /locus_tag="VGR22_08825"
     CDS             complement(12530..>13036)
                     /locus_tag="VGR22_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128707.1"
                     /translation="IIDRTTGTVTFRRVTVISITADRPATLEAGIVVALVGLATAIGY
                     RTDVTMAIAGALIGWGGLASAIWFIADRFMSTPTSREDFQPLVRTIGYATAPAMLNVI
                     HFIWWLGPLAAGFGTLWAFAATAFAVRQTTRFGWLRVIALTVGGGIVVNVLGFMLAAI
                     AGIAPQVW"
BASE COUNT         2205 a         4171 c         4414 g         2246 t
ORIGIN      
        1 cccgatgagg atctcgccag atgtcggctt ttccaggcca ccgatcatgc ggaggagcgt
       61 ggtcttgcca cacccggacg ggccgagcag caccacgaat tcgccatcct cggttcggag
      121 atcgatgccg ttgaccgcgg gggcgccctc accgaagtcc ttgactagtc cccgcgtttc
      181 gactcgtgcc atggtgtcct gctctgttct cggtcgttcc atgatgcgga ccggcaggat
      241 cgacgccgcc ttcgctgggc atcgatgcta ccggtccacc acggaatccc tcgtcgcggg
      301 tcggcggtac gcctactggc cgccgcccat cagcccttcg tcacgccacc gctgggcgat
      361 ctcctcaagc ttcgcctcgg cgtcagcgac cgcctcctcc ggcgtcatgt cgccgcgagc
      421 ggcctgtgcc atcatgtcgg aaaggatgaa ggtgttgaag gcttcgccga tcatggcgtt
      481 ggctggcccg ggccagccga ggttgacggt ccactcctcg gcggtcttca gcggcgccag
      541 cttgtcgggc ggcacggagc cgctggggtc atcgtccagc gggcccccgt cctcgacaag
      601 ggatgggaag gtctccggga aggccgggaa gttgtagagc tcgctggcgc ggctcgccgc
      661 ctcgtagttg gcgacgaggt ggagcaggaa ctccttggcc gtgtcctggt tctgtgagta
      721 ggtcgggatc atcgcggtga agaccgcgtg accgtgcgcc agcgcccggt cctcgcctcc
      781 cgggccgacc agcggcgtgc tgaagaagat gtcctcggcc acctccggct gctgcttctg
      841 ggcagtgcgg taggcggaga tcgagttgag gatgtaggac gcctggccgg cgacgagcaa
      901 ctggttgttg ctggcggcgt tccagccgaa gacctccggc gtcatgcagc tctcgaacag
      961 cgtcttcatg taggtaacgg cttcgacgga ctcgggtgag ttgatgatga tgttctcgtt
     1021 ctcgtcctgc acagctgcgc cgaaggccca caacatagcc tgcgcggcca tgttggagtc
     1081 gatctcctgc gacatgccga tgcccatctg gacgccctgc tcgttccaga tctgggtgcc
     1141 gccatcgagc agttcgtcat aggtggaggg gccgttctcc agcccaaccg tttcccacag
     1201 gctcttgcga tagttggcgg gatccggggc gtagccgtgg cagaagccgt agtacttgtc
     1261 ggtggtcggg ttccacgagt tgcgcttggc catctcgagc tgcggcccgt ggcggcgctc
     1321 tgcttcctgc accagctcgg tcaggtcgag gacggactcc tcgtactggg ggagcgaggc
     1381 aatgtgctcg acgatgtcat gaccctgctc ggcgctgatc tcggccgcga aggtcgccgg
     1441 cacgtcagcg gtgttgatca tgtcgacggt ggccgtgacg ccgtttgcat cagcccactc
     1501 ctgcaggaac tgcgggaacc actcatcgaa ggcggggacg aagtgcgacc actgaaggat
     1561 gctcagggag gtcccggaga tgtccaccga cggcgtcgac tgcacgagtg tcgcggcacc
     1621 cgtaccggtc gcgcgggtgg cgaggtaggt tgccacgccg agacttccgg caccctgtat
     1681 gagtgttcgg cgcgaaaatt cagtctggtg cgaacgacct gcggaagtac gacgaatcat
     1741 gacagatcct ccttgtacgg tcggctcttc actttgccca ctgggccgat gtgccaatac
     1801 cataccccat ccatggtgtt ttgtcgctac cccttttggg cacgtgctta cggaatggtt
     1861 gggtgagggt caatcgcgga tcgcatgcag gtcggcgtgg gcggtgtcgg ccgagacctc
     1921 gcgcagcgcc tccgaccatg cctgcaggct gtgggcgacc tggtccaggt cgcgctggtc
     1981 ctccagcccg gtgacgatca gcgccgcgag gctgccgcgc gcttcctgca gggcgtccac
     2041 cagccgctcc aggctgtgct gcttgcgaat cagcgcctct tcgagctggg gcgaggtcgc
     2101 tccggcacgc gcggcttcca cgacgcgatc ggcgttgcgg ttcagcaggc ggccggcctg
     2161 tcgcatgttc ccgtcatatg accgaatgat gtcggccggc agatgaactg cctgggccga
     2221 ttgcagcgtc cggcgcatcg ccgccagctc ctcgttggcg accagcagga agctgcgaat
     2281 cggccaggtc ttcgggatgt tctccgtgaa gtagctggcg gggcgcggcc atgggtactc
     2341 cgtctcccgg aagccgctgc ggatggagtg ccagattgcc tcgcggctcc agggcgtgcc
     2401 gggtggcgcg gactgcacct tcactactac gccgagcgcg aggatgagcg acaccagcgt
     2461 gatgaggagg gaagcatcca tgacgaaacc tccggggtgt ggctagtaca tcagcgggat
     2521 gatggtttgc tcgatgatcg ggcgtggggg atagagcggc accgagatgt cgcccaccgc
     2581 gatggcgatg accagctcga gcgccggcac cacccagatg tactggcctc cgtatccgag
     2641 cgcatagaag gcatcgtagc cggtccaggt cgtcgtgacc caccactggt agccgtaggg
     2701 cgtaccgccg gtcggatcac cactgctctg cacgatgatg ctctgttcga tgtattccgg
     2761 cgcgatgagc tgctcgccgt tccatgcccc gtcgcgaagg tagaggtagc cgagccgcgc
     2821 catgtcctcc ggcacgatct gcagcccata accgccgatc acctcgccct gtggcgtccg
     2881 gcgccagccg tgcatggtga tgccgagcgg cgcgaagagc gcctcctcgg cgtattcctc
     2941 cagcggcatc ctagccgccg cggcgaccat tagcccgatg atatgggagt cgccgctgtt
     3001 gtaaacgtag gccgttccct gcggcgcggc gatcggcagt cccagtgtga gcgcgaccgg
     3061 gtctgctgcc gcttccagcc gggggtagtc gtcctgccag gtccacgcca gccccgacgt
     3121 catcgtcagc aggctccaga gggtgatgtt cgccacggcg gggtcggcgc cctcggggat
     3181 ccgttccggg atcagctcgc cgatggtcac tgtgagatcg gggagcagac cgtcatgccg
     3241 ggcgcgaccg acgagcgcgc cgacgacgct cttggtacac gagcggatat cgatcgggtc
     3301 gtcgggcccc cagccgtccg cccagtaatc ggcgacgatc cggccggcac gagtgacaac
     3361 tacgccggtc acgctcggcg tggctgcacc ggcgttcgcc aggagctctg gcagggccgg
     3421 gtcgacgttc atctcggcgg agttcgtccg tggccagtcc ggcactggcc agacggtcgg
     3481 acccggctgt tgcgactggc cagcagaggc gatgggcacg atggctgccg cgagtgccgc
     3541 gccgacgagg ttgcgacgat tgaggtgagg catggggtgt cctttccctg cgcgtcctga
     3601 ctttatccta cgctacggca cgcggctccc atcagaagtg cgcgggattc agtatttatg
     3661 ccgcaattgc ctgatgcctg tatgatagat acaataaaga cagcaacaca tcgcaatcgt
     3721 tcgaggcgcg gctggaggga ctgccgtccg aacgctgaac gaacggatgc gagcgctggt
     3781 cggggcagct cctgccctgg ctgtgatcct cgtcgggtgt gtggctggct ccactcggcg
     3841 agatccggaa aacctgatgt atcggctgac gccgcagctg ctcaggacga gcttcgtggg
     3901 gagggagcgc gacgcccggg cgatcgcgga tctgtttgcg cgaggcgatg tccggatcgt
     3961 aacgctgacg ggaccaggcg gggtagggaa gacccggctc gcttttcatg tcctcaatcg
     4021 gctcgagagc gcgttgccgg atggcgtcgt gatcgtcgat ctggccacga tcaccatccc
     4081 ggatctcgtg ttgcccgcga ttgcgcacga gttcgggatc atggagtccg gcgccgagtc
     4141 gatcatggcg cgcctcgtcc gcgtcatcgg ccagcgccgg actctcctgc tggtcgacaa
     4201 cttcgaacag gtggctgagg ccggcatggt gatctcccgg ctcgccagcg actgcccgaa
     4261 cgttgccttc ctcgtcacca gtcgcatgcc gctgcatatc gccggcgagc acgagtacac
     4321 cgtcagcccg ctggagacgc cacccgtcag gggcaactcg ccgaacggtg tcatgctcga
     4381 tggtgtgccg actgaggagt atccggcggt gcgcctcttt atcgagcgtg cccaggctgc
     4441 ccaccgggcg ttcgccccta cccctgagat ggtccggacg gtcggcgaga tcaccgcgct
     4501 gctcgacggg ttgccgctgg cgatcgagct ggcggctgcc cgcaccaagc tcttctcctt
     4561 gcaggcgctg ctcaaccgcc ttgacgatcg catggggctg cttaccggtg gtccgcgcga
     4621 tctcccggat cgtctccaga cgatgcgcaa tgcgatcgcc tggagctacg acattctcag
     4681 tccggaggag aaggccgtct tccggcgcgt atccattttt ccgaacagct tctcgctgga
     4741 agccgccgag gcggtggtgg ctggaccgct cgccgagcag gagcgtgcct ttgtcgagga
     4801 gtacgacggg tccccgtccg atctcgaacc aatcggcaac gtgctgggtc atctcgagtc
     4861 gctggctgac aagagcctgc tgcaggcggt gaccggattg cacgaggatg ttcgcttccg
     4921 gatgctcctg accatccagg agtttggccg agagcagctg cgtgctgagc gtgagctcct
     4981 gctgatgcgg atgcgcctgc tggtgcactc ctatgaacat ctggcgaacc tcgacgagct
     5041 gctcttcggc cctgagcaac gcatctggct cgatcggctc gatcttgagc agggcaacat
     5101 ccgcggggcg cttcagttcg cgctcgagta cccgcgcgag ttcggcgtgc agggcgtcca
     5161 tctcgcctcg tcggtctggc gctactggtt agtgcgcggt catgttgcgg agggagcgcg
     5221 atggctggag caggcgttgg cctgccgtga cacggtcgag attccggtac tgatagaggc
     5281 cgaggcggtc aaccgcctcg gcaatttgct gctggatctc ggcaagcacg aggaggcgga
     5341 gcgccattac cgggagagcc tggcgctcta ccgctctgcc gatcatcgtg atggcgttgc
     5401 cgatgagctc aacaatctcg gcctggtgca gttgctccag ggcaggatcg agcgggcgag
     5461 atcgagcctg gaggagagtc tcgccctgcg tcgcgatggt ggcgacccgc gcgccctgcc
     5521 caacacgctc tccaacctcg gtgacctcgc tatcgaggag cggaactatg atcgcgccga
     5581 gagcctgcac atggaagcgc tcagcatccg gcgagagatc ggcaatctgc gcggcatggc
     5641 cctcagctgc tgctcactcg gacaggtcgc cttcttctgg aaggagttcg accgtgcgcg
     5701 gaggtggttt gacgaggggc tggagtacca gacccaggtc gacgactcgt acagccttgc
     5761 gatgctgatg ctggggctgg ggcggctcga cctcgcgacc gggaggccgg ttcaggcgat
     5821 gaagcggctc ggccgcgcgc tggaggtctc ccagcgcatg ggctcccggc gcatggtcct
     5881 ttccgtgatt gacacgattg ccacctcggc cgtgagctat ggtttcgacc gtgacgccat
     5941 tcgcctcttc ggcacgacac aaggggtgcg cgagcgggag aacctgcatc acccgcagcg
     6001 agagtgtgcc gccaccgatc gcgtgcttgc aatgctcacc cagcggctcg gcgagtcggt
     6061 ggtctcgcgg gagcacacga tcgggcgacg gcagtggctc aaccaggcgg ttcaggaggc
     6121 ggctgcgctc attgtccaga tccaggagcg actggagaca ggcgaggtgg gtactgccgg
     6181 cgtcaacggg gtcttcgatc ccgccgccgc gactcgtaag gcaagggcgt tcgggctcac
     6241 acgccgggaa cgccaggtgc tcgccctgct agtacgcggc gcctccgaca aggagattgc
     6301 cgaagcgctc tccatcgcgc cgcgtaccgc gatgacgcat gtctccaatg tcctcgccaa
     6361 gctcggtgtc aaccgccgaa ctgccgccgc cagctatgcc atccgggagg ggctcgtcga
     6421 tcccggcgcg acccgcgagg ggtaacctcc aatctcgtaa tctgggctga aaaccggcac
     6481 aaaaccgggg ttgacaggcg ttcaccatgg tgggacggta acgccggatt ggcccctccg
     6541 catggtatgc ttgggggcaa ccctgggagt tgtaccgtcg gcacagacca tgcgatcttc
     6601 tcacgcagac gagtttacag cctccgccgt gggctgcggt cgatggcatg agaacgacag
     6661 cgaacaagta gcagacgtgt cggcccacca ggcggtggga gcgcgcgtca gggagccgaa
     6721 cggacagtat catgagcgac ctgcacacgt ttttcgggcc caacgctggc tacgtgctgg
     6781 agctctacga tcggtatctg gctgacccgg ggtcagtcga cgccgagagc cgggcctttt
     6841 tcgagtcctt cacgccgccg accgaagacg gggctcagcc gacgcccggc gtggccacgg
     6901 cggttgccgg gagcaccgtc gacctgttga aggtcgctgc cgcggtcaat ctggcgaccg
     6961 gcatccgcga gtatggccac ctggcggtgc cgctggaccc gctggggagc gagccgcctg
     7021 gagcgcccga gctggaccct gccacctatg gcatcaccga ggacgatttg aagagcacgc
     7081 cagcggagat cgtcggtggc gaggcggctg acggggccgc caacgcattc gaggcgatcg
     7141 agcgcctccg tcggcggtac acgacccgga tcgggtttga cttcgaccac gtgcaggagt
     7201 ctgacgagcg tatctggctc aacgatgccg tcgagtcggg ccggttcgaa gtcgagccgg
     7261 atgaggcgtc ccagcgcaag ctgctggagc ggctcaccca ggtcgaggtc ttcgagcgct
     7321 tcctccacca gacctatctc ggccagaagc gcttctccat cgaagggaat gatatcctcg
     7381 ttcccatgct ggaccagatc acggacgacg cctcggctgc tggcacgcgc caggtgtttc
     7441 tcggtatggc ccaccgcggc cggctgaacg tgatgaccca tattctcagc aagccctatg
     7501 gcgcgatcat cgccgcgttc gagggcggca agcgtcgagc cgctgccagc acggagtcgg
     7561 ccgatgacgt ccgcaccggt gacgtgaagt accatctcgg cgctcggctg gcccgcaacc
     7621 cggaaaccgg cgagcgcgtc aagatgccgg tcgtgctggc gccgaatccg agccacctcg
     7681 aggcggtcaa cccgatcgtg ctcggaatga cccgcgcagg gcaggacgac cggaccacgc
     7741 ccggccgctc cgagcaggac acgtcgtgct ctctctcgat tctgctgcac ggcgacgccg
     7801 cgttccccgg acagggcgtg gtggccgagt cgctcaacct ctcgagcctg gccggttatt
     7861 cggttggggg gaccatccac atcatcgtca acaaccagat cggctacacc accgactggc
     7921 gcgatgcccg ctcgacgctc tacgctggcg acctcgccaa gggcttcgag atcccggttg
     7981 tgcatgtcaa cgccgacgat ccgctcgcgt gcctgcaggc agccagcatg gcgatggcgt
     8041 accgtcgcca gttcggcaag gacttcctga tcgacctggt cggctaccgc cgctggggcc
     8101 acaacgaggg tgacgagccc gccttcacac agccggtgct ctaccagaag atcagcaagc
     8161 acccgacggt gcgcgccatc tgggccgatc ggctggccga gaagggcgtg ctcaccgagg
     8221 acgacgctgc ggccatggag cggcgcatgc tcgaccggct cgcgaagatt cgtcgcggca
     8281 tcaccgaagg tcaggatacc tacgacgaac aggtggagct gacatcagga ccgcgcgagg
     8341 aggtcgagac cgctctctcc gaggaccggc tcaaggcgct ggatgccggg gtccacaagc
     8401 tgccggagga tttcactccg aacagcaaac tcaagcgact ctgggagcgt cgcgcgcaga
     8461 tcgtccagac gggcgagggc aagatcgact gggcccatgc cgaggcactc gccttcgcgg
     8521 cgctgctggc cgatggtgtg ccgatccgcc tgaccggtca ggatgcgcag cgtggcacat
     8581 tcagccagcg gcacatcgtg ctccacgacg ccaagaccgg cgaggcctac gcgccgctgg
     8641 cgcagctgcc ggaggccaag gcctcgttcg ccgtctacaa cagcccgctc tccgaaaatg
     8701 cggcgatggg cttcgagtac ggctacagcg tgcaggcgcc cgaggcgctt gtgctgtggg
     8761 aggcgcagtt cggcgacttc gtcaacagcg ctcaggtcgt gatcgaccag tttctctcct
     8821 ccgcccgctc gaagtggggc caggagccaa cgctcgtgct gcttctgccg cacggatacg
     8881 agggacaggg tccggagcac tccagtgcgc gccttgagcg tttcctgcag ctcgccgccg
     8941 gcgataacat gcgtgtggca aacgtcacca gtgcggcaca gtacttccat ctgctccgcc
     9001 gccaggctgc caggctcgat cgcgatccgc gaccgctggt gctcatgacg ccgaagagcc
     9061 tgctccggaa cccgatggca tcgtccagcc cggagcagtt cacggagggt accttcctcc
     9121 cggtgatcga cgacgcgaag gcgcaggacc gcaaggacag ggtgacccgg ctggtcctct
     9181 gcacaggcaa ggtcgccatc gaccttgagg ccagcgacca gcgcgacgag acggagcacg
     9241 ttgccatcgt ccgggtcgaa cagttggccc cgttccagag ctcgatcctc cgggacgtca
     9301 tcgacagcta cccgaatctc gaggagatcg tctggctgca ggaagagccg aagaacatgg
     9361 gcgcgtggtc gttcatggtt cagcgcctgc gcgacctgat cgaggacgcc tacccggtgc
     9421 ggtacatcgg acgtaccgag cgcgcggcgc cggcggaagg ctcgctggag cagcacaacg
     9481 aggagcaggc ggcgatcgtc gcgggggcgt tcgagcccca gcgcgctgcc ggcggcgcgg
     9541 cgacgaacgg tgccgcgcca tctggccgga agcgagacag caagaagggc gcgcgcgatc
     9601 gggcggcggt tgccgcatcg cccgccgagt agcgagaagg acgccacacg ggtgacgatg
     9661 tggcgagggg gcgtgacggc gcacccggcc ccgcatgagt tgaggagaac aaggcgcata
     9721 tggcgatcga aatcaaggtt ccgccgctgg gagaatccct ggtcgacgca gtcgtcggcc
     9781 agtggctgaa gtccgagggt gacgcaatct ctcggggcga gacgatccta gagctggaga
     9841 cggacaaggt caacctggac gttaccgccg aggacgatgg tgtgctgggc gccatccaga
     9901 aggaggaggg cgacaccgtc accgtcggtg aggtgcttgg cgtgatcgag gccggtggcg
     9961 gcaacggggc cggcgcgcca gccgaggccg aagccgagga gccggctgag gtggaggccg
    10021 cgtccaccgc gcccgccacc gagccgcaac ccgagccggc ccccgcagct gctgccaccg
    10081 aggaggctcc cgagccgtcg gctgctgaag atggcgccgg tcgtgccgcg cccgccgtgc
    10141 gtcgcatggc agaggagcac gcgatcgacc tcgccaaggt ctccggcact ggcgccggcg
    10201 gtcggatcac ccgtgatgac atcgtcgcct atctcgccag ccagcgcggg cagggcgggg
    10261 cagcagcgcc tgtcgccgct ccgccgcagc cagagacagc ccagccgacc cctgcccctg
    10321 cgcctgccga acagcgcgaa gacgaggagc gaatccggct ctcgcgccgt cggcagacga
    10381 tcgccaaacg gctcgtcgag gcacagcaga ccgcggcgat gctcacgacc ttcaacgagg
    10441 tcgacatgtc ggcggtcatg gacctccgca agcgccgcaa ggaatccttc aaggagcagc
    10501 acggcgtcaa ccttggcttc atgtcgttct tcaccaaggc ggcagtgggg gcgctgaagg
    10561 ccttccctga ggtcaactcc gagctgcagg gcgacgagct gatcctgaag aagcactacg
    10621 acatcggtat cgccgtcggt gtcgaggagg ggctcgtggt tccggtcgtg cgcaatgccg
    10681 accggcgcag cttcgcggac atcgagcggg agatcatcga gcttgcgggc aaggcgcgcg
    10741 agaacaagct ggcgatcagc gagctgcagg gtggcacctt cacaatcacc aacggtggcg
    10801 tgttcgggtc gttgctctcc acgccgatcc tcaacacgcc gcaggtgggc atcctcggca
    10861 tgcacactat ccagcagcga ccggtcgcgg tcgatggcga ggtggtgatc cgtccgatga
    10921 tgtatgtggc gctctcgtac gaccatcgga tcgtcgacgg gcagcaggcc gtgcagttcc
    10981 tcgtcaagat caagcagatg gtcgaggatc ccgagacgct ccttctcgag ggatagagat
    11041 ctgcctgata accgaacgaa aaccggggag gggtacagag acgcccctcc cccgtcttgg
    11101 cgtccgttgg cgcaggtgtc ggcaggcccg gaaatcaagt gttgggggta cacgaaatct
    11161 cttgccgttc cagccgtcag aacttctcgt ggatgtcggg aatcgcaggc gggaagagcg
    11221 cgcggagggt tgcctgcgtc tcggtatcgg gatcgcccca gccacggaag cagaagcctg
    11281 ccggatcatg cccggtgaag acgagtgccg agagtccctg gatcgtcagg tgtccagccg
    11341 gcttggcatc agtcgcctcg accgccagca cgccgctctc ggcccgcaga gtgaagatac
    11401 cgttgttcca cggacatatc agatcccgga tctcgagcgt gatctctccc tgaccagcgc
    11461 cgatgccgct cagcttttcc acgtcgacca ctcgacccat cgggccgggc catgcacgct
    11521 cggcatcggt ccgcgtggct tcgcccagat catggaacca gacctccggg tactcgtccg
    11581 gccggagctc aatgacggcc tccctgacct gatcggtgtg gcgaccgatc cagtccagca
    11641 gcagataccg gccgatgctg ttcaccgtgt agaacgtgtc tgcgatgagc ttttccccat
    11701 agccggtgat cttgaatgtc atcgccccga ccacctcgcc ttcgtaccgg gcaagtgcca
    11761 cccacgactc gttgaggact cgcgcctgac gagccttggt gacatgggcg agggcaaagc
    11821 catgtgtgtg ccgctggaac cgctcgagaa aggcccgcca ctcatcgaaa ccgtccttca
    11881 tggcgagttg ctcgcaggtg cccggcttgt catggcgaac cagtggcacg agtgcttccg
    11941 gtctcagtgt gaggaaccga ggcttgggga agctggcata gccgagccgc tcgtagaatg
    12001 agtcgcgaaa cgggtagagg gttgcgctcg gcatctcggc gtcgtggtag agctcgaaca
    12061 cccgctcgaa catccgcctg actattccct gccggcgccc gaccggcatc gaggcgacac
    12121 cgccggcgcc gcccatcggg accaccatgc cacgcacgtt ctgcgtcatc tcgtggctca
    12181 ccatcgatgc ctgggcgccc ccagcgacga acgcgatgag gttggtggtc cgctcgtagt
    12241 acggcaggtt ctgccgcgcg cgttccatgt cggtttgctg tggcgacgga ccgaaggcat
    12301 agctggcgat ggggcggccg gcttcgaggt attcctcgct ggagaccgtt cggatctcga
    12361 tcagggcggg gttgctggcc atgcgacggt gcatcctttc gaccactagg aacggcggcg
    12421 ctgcggttgc cgggagccat ggtgccatat tgaaacccgt tcgggtcgat gcatggcgac
    12481 gccccgactg ctgcagtcgg ggcgtcgtcc cgtggggagg gggagcaggt taccagacct
    12541 gcggggcgat tccggcgatg gctgcaagca tgaatccgag cacgttgacc acgatgccgc
    12601 cgccaacggt aagcgcgatg acgcgcagcc atccaaatcg ggtggtctgc cggacggcga
    12661 aggcggtggc cgcgaaggcc cagagcgtgc cgaagccggc ggcgagtggt cccagccacc
    12721 agatgaagtg gatgacgttc aacatggcgg gagccgtcgc atatccgatg gtccgcacca
    12781 gtggctggaa gtcctctcgt gaggtcggcg tgctcatgaa gcgatcggcg atgaaccaga
    12841 ttgcgctggc gagaccgccc cagccaatga gtgcccctgc aatcgccatg gtgacatccg
    12901 tccgatagcc gatcgcggtc gccagcccga cgagagcgac gacgatgccg gcctcgagtg
    12961 tggcggggcg gtcggcggtg atgctgatga cggtcacccg ccggaacgtg acggtaccgg
    13021 tggtgcggtc aatgat
//
LOCUS       DASYWL010000238         4222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_462337, whole genome shotgun sequence.
ACCESSION   DASYWL010000238 DASYWL010000000
VERSION     DASYWL010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462337"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="VGR22_08830"
     CDS             complement(<1..712)
                     /locus_tag="VGR22_08830"
                     /inference="COORDINATES: protein motif:HMM:NF042610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6789 family protein"
                     /protein_id="HEV2128708.1"
                     /translation="MSDYSTRTTRFEGGWRSDWIRSGILAGFIATFAMTATLTVAWLT
                     ANALSDPDGGQLSRWIYNLSENRIVDRVGGAFLIGLALNLIVGIAWALLYARFVEPWF
                     GRSTIVTGMLFAMIPFVISVAVIFPLMNAGFLGSELDAGPLPFIGNLVLHLVYGGTLG
                     LLYGIESERGITNRASERRANATAELGAAYGVVIGGIVGAIGGWLVAPTMDDLADRPV
                     ITLAGVLTGAAIGTLIGSF"
     regulatory      870..977
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1117..1818
                     /locus_tag="VGR22_08835"
     CDS             1117..1818
                     /locus_tag="VGR22_08835"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV2128709.1"
                     /translation="MTESCSSPTEPETPPSRRHLRVLRSDRRTADGVLRHTSIGPLDL
                     EMGGHLEEITVAWRSWGQLNERGDNAVIVLHALTGDSNAAGEGGWWEPVIGPGRPIDT
                     DHHYVICSNVLGGCQGTTGPASTDPETGRPYGIRFPLVTIGDMVTVQRRLIESLGITA
                     VIPIGGSIGGLQALEWATRHPDLVRSSGVVAASSALGAQGIALHGEVGRRAIMADPNW
                     CQGDYAEYGVFPEQG"
     gene            1830..2309
                     /locus_tag="VGR22_08840"
     CDS             1830..2309
                     /locus_tag="VGR22_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128710.1"
                     /translation="MAAMVTYHSRDSLGSRFGRNPASRPVLYPGIGPTFDVEGYLHYH
                     GQALVQRFDANSYLYLTRAMDMYDVGRDGDEETWLRRIDAPVLLVGISSDWLFPPDDI
                     QTLTANLQRLGADATYCEIDSPNGHDSFLKDWDQLNAIVEPFISRLRSATAAKEAAP"
     gene            2443..3753
                     /locus_tag="VGR22_08845"
     CDS             2443..3753
                     /locus_tag="VGR22_08845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HEV2128711.1"
                     /translation="MSENATKSLGFETRALHAGQQPDPTTSSRAVPIYQTTSYVFEDT
                     EHAADLFGLRALGNIYTRIMNPTTDVLEQRIAALEGGTAAVAMASGQSAETLAILNIA
                     GAGDEIVSTTSLYGGTYNLFHYTLPKMGIEVKFVEPTAEAVKAAINDKTKLVYSETIG
                     NPDLNTLDIRAVGDVAHAAGVPLMIDNTTPTPYLIRPIEHGADIVVHSLTKFLGGHGT
                     SIGGVVVDGGTFDWGSGRFQKEFVDPDPSYHGLSFWEVFQDFAGMGNVAFAFKLRTQG
                     LRDVGPAISPFNAFLILQGIETLPLRMERHSENAMQVARFLTEHPKVSWVNYPGLPGH
                     KRYDVASKYWDHGLFGAIIGFGVKGGLEAGRTVIESVEIFSHLANIGDAKSLIIHPAT
                     TTHSQLTEAEQADTGVSPDYIRLSIGLETVGDLIADLEQALDKI"
     gene            3943..>4222
                     /locus_tag="VGR22_08850"
     CDS             3943..>4222
                     /locus_tag="VGR22_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128712.1"
                     /translation="MITPHRIGDLEVDEDIEFQEREWKVQRVGWALMLVVIVLALVGL
                     FGTGPLSAVEREADDGTLVLEYQRFVRHGGRTTFSIAAAGSQAENGEIE"
BASE COUNT          787 a         1445 c         1299 g          691 t
ORIGIN      
        1 cgaacgagcc gatcagtgtt cctatcgcgg cgccggtgag caccccggcc agcgtgatca
       61 ccggccggtc agccagatca tccatcgtcg gcgcgacgag ccagccaccg atcgctccga
      121 caattccgcc gattaccacg ccataggctg cgcccagctc ggcagtcgcg ttcgcacgac
      181 gctcgctggc acggttggtg atgccgcgct cgctctcgat cccgtacagt aaaccgagcg
      241 tgccaccgta aacgagatgc agcacgaggt tgccgatgaa cggcagtggg cccgcatcga
      301 gctcgctccc caaaaaccct gcgttcatca gcgggaagat caccgccacc gagatcacga
      361 agggaatcat cgcgaagagc atgccggtga cgatggtgga tcgcccgaac cacggctcca
      421 cgaagcgggc atagagcagg gcccatgcga taccgacgat caggttgagc gccagtccga
      481 tcagaaaagc gccacccacc cggtcgacga tgcggttctc cgagaggttg taaatccatc
      541 gggagagctg gcccccatcg gggtcactga gtgcgttggc ggtcagccat gcgacggtga
      601 gggtcgctgt catcgcgaag gttgcgatga atccggccag gatccccgaa cgaatccagt
      661 ccgatcgcca gccaccctcg aaacgcgtcg tgcgggtcga atagtcgctc atgcctccac
      721 tcctgtccga ggcgtgccac acataccctg tccgatgcct ccatcttatc ggcggggtct
      781 acagattatg tatcaggagg taccgcggag gtcgcttgac agcggcgcag atgcccacgt
      841 agcatggaac tcaacaggtg aatagcactg ccttatccag agaggtggag ggaatcggcc
      901 ctgtgaagcc cggcagcctg cgacgcgtgt acgtgcgtaa ggtgccaact ccggcagagt
      961 gatctgcgag ataagggatg gcgtggatac gccccctgca tcagtgcaag gggcgttttt
     1021 cgtatccggc tcccggacct cgcggcaccc ccttcctctc cactgccatc cgggcgcgaa
     1081 cacgcccgtc agtccaggaa agcgagagtg cggcccgtga ccgagtcctg ctcgtcacca
     1141 acagaacccg aaactccacc gagccgccga cacctgcggg tcctgcggtc ggaccggcgc
     1201 accgccgacg gtgtgctgcg ccacaccagc atcggcccac tcgaccttga gatgggcggc
     1261 cacctcgagg agatcactgt cgcctggcgg tcctgggggc agctcaacga gcgtggcgac
     1321 aacgccgtca tcgtcctgca cgccctcacc ggcgacagca acgccgccgg cgaggggggc
     1381 tggtgggagc cggtgatcgg ccccggccga ccgatcgaca ccgaccatca ctacgtcatc
     1441 tgcagcaatg tcctcggtgg gtgccagggc accaccggtc cggcctcgac cgacccggaa
     1501 actggcaggc cgtacggcat ccgcttcccg ctggtcacga tcggcgacat ggtcaccgta
     1561 cagcggcggc tcatcgagtc cctcggcatc actgccgtga tcccaatcgg cggttccatc
     1621 ggcggcctcc aggcactcga gtgggccacc cgccatcccg atctcgtccg tagcagcgga
     1681 gtggtggccg ccagcagcgc gctcggtgca cagggcatcg cgctgcatgg cgaggttggc
     1741 cgccgcgcga tcatggcgga ccccaactgg tgccaaggcg actacgccga atacggggtg
     1801 ttccccgagc agggttgacg atcgcgcgca tggcggcaat ggtcacctac cacagccgcg
     1861 acagcctcgg ctcccgcttc ggccgcaatc ccgccagccg cccggtcctc taccccggca
     1921 tcggcccgac cttcgacgtt gagggctacc tgcactacca cgggcaggcg ctggtgcagc
     1981 gcttcgacgc caacagctat ctctacctga cccgtgccat ggacatgtac gacgtcggtc
     2041 gggacggcga cgaggagaca tggctgcggc gcatcgacgc ccccgtgctg ctggtcggga
     2101 tctccagcga ctggctcttc ccgccggacg atatccagac gctgaccgcc aacctgcagc
     2161 gtctcggcgc tgacgccacc tactgcgaga tcgactcgcc caacggccat gacagcttcc
     2221 tgaaggactg ggatcagctc aacgccatcg tcgagccgtt catcagtcgc ctgcgcagtg
     2281 cgaccgcagc gaaggaggcc gctccgtgac tgccccgatg catcgcgagc ggtatcaccc
     2341 gctggcgcgc gactacctga tgcccaacag ggcatggcac gcgcgcagcc accctgtccg
     2401 tcattgatca gccatcagca gacaccgtcg aaaggatcac ccatgagcga gaacgccacg
     2461 aagtccctcg gtttcgagac ccgcgccctg catgccggcc agcagccgga cccgaccacc
     2521 agctcccgcg cggtgccgat ctaccagacg acctcgtacg tcttcgagga caccgagcat
     2581 gccgccgacc tcttcggcct gcgcgccctc ggcaacatct atacccgcat catgaatccg
     2641 accaccgatg tgctggagca gcgcatcgcg gcgctcgagg gcggcaccgc agcggtggcg
     2701 atggcgtccg ggcagtcggc ggagacgctg gcgatcctca acatcgccgg cgccggcgac
     2761 gagatcgtct cgaccacaag cctctacggc ggcacctaca acctcttcca ctacacactg
     2821 ccgaagatgg gcattgaggt gaagttcgtc gagccgacgg ccgaggcggt caaggcggcg
     2881 atcaacgaca agaccaagct ggtctattcg gagaccatcg gcaaccccga tctcaacacg
     2941 ctcgacatcc gggccgtcgg tgatgtcgcc cacgcggcgg gcgtgccgct gatgatcgac
     3001 aacacaactc cgacgcccta cctgatccgc ccgatcgagc acggggccga catcgtggtc
     3061 cactcgctga ccaagttcct cggtgggcac ggcacctcga tcggcggcgt cgtggtcgat
     3121 ggcggcacct tcgactgggg ctcgggacgc ttccagaagg agttcgtcga tccggacccc
     3181 agctaccacg gcctgagctt ctgggaggtc ttccaggatt tcgctggcat gggcaacgtg
     3241 gcattcgcct tcaagctccg cactcagggc ctgcgggatg tcggtcctgc catcagtccg
     3301 ttcaacgcct tcctcatcct gcagggtatc gagacgctgc cgctgcggat ggagcgccac
     3361 agcgagaacg cgatgcaggt tgctcgcttc ctgacggagc atccgaaggt ctcgtgggtc
     3421 aactaccccg gcctgccggg ccacaagcgc tacgacgtcg ccagcaagta ctgggaccat
     3481 gggctcttcg gtgcgatcat cggcttcggt gtcaagggcg gcctcgaggc ggggcgcacg
     3541 gtgatcgaga gcgtggagat cttctcgcac ctcgccaaca tcggtgacgc caagtcgctc
     3601 atcatccacc cggccaccac cacccactct cagctcaccg aggcggagca ggccgacact
     3661 ggcgtctcgc cggactacat ccgtctctcg atcggtcttg agaccgtcgg tgacctgatc
     3721 gccgatctgg agcaggcgtt ggacaagatc tagtccaccc cgctgtaccg gccatcccga
     3781 gcgagatggc cagaccgatc ttgcaagtcc tcctcaaccc aacgcgcccc ttgccgcctg
     3841 aatactggca aggggcgcgt tgttgctgga cagtagtggc aaggggattg ctgtatacgt
     3901 acagcatgat acgttagcat ctgtatggag gcttgcgcag gaatgatcac accacatcga
     3961 attggagatc tggaggtcga tgaggacatc gagttccagg agcgcgagtg gaaggtccag
     4021 cgggttggct gggccctgat gctcgttgtc atcgtgcttg ccctggtcgg actctttggc
     4081 accgggcctc tgagtgccgt tgagcgggag gccgacgacg gtacgctcgt gcttgaatac
     4141 cagcgcttcg tccgccacgg cgggcgcacc accttctcaa tcgctgccgc cggtagccag
     4201 gccgagaacg gcgagatcga gc
//
LOCUS       DASYWL010000239         4228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_48823, whole genome shotgun sequence.
ACCESSION   DASYWL010000239 DASYWL010000000
VERSION     DASYWL010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48823"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VGR22_08855"
     CDS             <1..461
                     /locus_tag="VGR22_08855"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEV2128713.1"
                     /translation="HADLLGELGVARHIDYTASRFEDETQPADVVFDTIGGDTFLRSF
                     DVLRDGGTLVSIAYFDEPPADLVERKRLRVRQFSMRPDRTRLETLARMVAQGELRPVI
                     GTLASLDDAIAAIRRAMRGHNQGNIVISMLQQGRVLGQRGTTADQSHRDG"
     gene            468..2177
                     /locus_tag="VGR22_08860"
     CDS             468..2177
                     /locus_tag="VGR22_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128714.1"
                     /translation="MSYEPATEGQEAGWTAVPEEEPYGTNLPRYVTQMIGRDADCERL
                     RQLLLEQRHRMVMLTGSGGIGKTRLAVEMASQLEDKWRHGVRFLSFAAVPRNTDLDAA
                     FAWHLGLGDTQGQSYRESVRQHLQLHEQLLVVDNIEHLPELADLLIYIQLYPAGVRGH
                     LHARHQPQGAPGVWGGGPPARPAACLRRGGRPASHPSRHRARAIDSGFDLTDSSVPDV
                     VGICDMLGGIPLALELIAPRLVTSAPEALLDELRETFDQAPGRPREPLEPAVTVQQTM
                     RLSYDQLDERQQRVFRVLSIMYGQWSVDDVLPLLTTEFDELEAVALIEVLVANSLISS
                     PPCPAGEVRFTVNPVLQHFGRSMLAAKGEHQTIADRHASRMVALAEEAEPELNGPNQQ
                     EWLTPLDALYDDFRHAHEHLRENGRRVDALRLATALWRYAYTRGHYRQPRRWIETSLG
                     EISGRDALRARSLTNGDFVESGEIPAKMLPAVADLLAEANGDAETLVELLQDRNVEGL
                     DAGVVDRLETWLLDKGSMTPATPLGHDEIRRRALREASGDLDVGSIGILDIDELLDYL
                     PIL"
     gene            complement(2324..2521)
                     /locus_tag="VGR22_08865"
     CDS             complement(2324..2521)
                     /locus_tag="VGR22_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128715.1"
                     /translation="MCAHFGENNMQDQPQLIQIRTTHEAPDDLVEPCGLPDHAEHNLV
                     VTPDSGCAAFTPADDQAPANA"
     gene            2798..4027
                     /locus_tag="VGR22_08870"
     CDS             2798..4027
                     /locus_tag="VGR22_08870"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV2128716.1"
                     /translation="MPIFRRARPGVAELARDEPFAPTPIYITPRVRLVLIIASFVVLY
                     LLAMVAPSIPRLLMLGGTLALSFPVRMLNRWVPRRWAIALAVGSTLFFAVLLLLILVP
                     FVITEVSGFIQDLPEIVEGAQDLTRDVIAAANERGWIRHDPDEIYDTVESTVIDRGQA
                     LIETTLAGALAALTRTFNILISTFGVFFIATYLLIDMPRFRQTFVNSFAQPYRRDAEH
                     LWRTLGESLSRYLGGLVASLTLQGIAAAVGLWLISVPYAVLLGIWTAMTAVLPYIGAF
                     LGAIPALFIAFTVSWQAAAGVVVLYVVINQVEANFIIPRIQGSAVRVHPLLIFLAVLL
                     GSELGGAFGAIIAVPMLAVVRVLAEFFWVRLRVRGAQQGTLLSAIGSEDVAPEAGPMV
                     LVEDDEPDRPVVRMDSS"
BASE COUNT          687 a         1377 c         1389 g          775 t
ORIGIN      
        1 gccatgccga cctgctgggt gagctgggcg ttgctcgcca catcgactac accgcgagcc
       61 gctttgaaga cgaaacgcag ccggccgatg tggtttttga tacgatcggt ggggacacgt
      121 tcctgcgctc atttgatgta ctgcgtgacg gtgggacact ggtttcgatc gcatatttcg
      181 acgagccgcc tgctgacctg gttgagcgga agcgcctgcg cgtgcgccag ttctccatgc
      241 gaccggatcg aacacggctc gaaacgctcg cccggatggt tgcccaaggc gagctccggc
      301 cggtgatcgg cacgctggcc agcctggacg atgcgatcgc ggctatccgg cgcgccatgc
      361 gggggcacaa tcaggggaat atcgtgatca gcatgctgca gcagggccgc gttctcggtc
      421 agcgtggcac aaccgcagat cagtctcatc gggacgggtg aggcatcatg tcgtacgagc
      481 cagcgacaga gggccaggag gcaggttgga cggccgtccc cgaggaggag ccgtacggca
      541 ccaaccttcc ccggtacgtc acccagatga tcggtcgcga cgccgattgc gagcgcctgc
      601 gccagctgct gctcgagcag cgacaccgca tggtcatgct caccggaagc gggggcatcg
      661 gcaagacgcg gctcgccgtg gagatggcca gccagctgga ggacaagtgg cgccatggcg
      721 tgcgctttct ctcgttcgca gcggtgcccc gcaacaccga tctggacgcc gctttcgcat
      781 ggcatctcgg cttgggtgat acccagggcc agagctatcg cgagagcgtc cggcagcacc
      841 tgcagcttca cgagcagctg ctcgtcgtcg acaacatcga gcaccttccg gagctcgccg
      901 atctcctcat ctatatccag ctatatccag caggcgtgcg cggacatctc catgctcgtc
      961 accagccgca gggagctcca ggtgtatggg gaggtggtcc accagctcga cccgctgcct
     1021 gtcttcgccg aggcggccgt cctgcctcgc atccatctcg gcaccgcgcg cgggccatcg
     1081 acagcggatt cgatctcacc gacagttctg taccagacgt cgtcggcatc tgcgacatgc
     1141 tcggtggtat tcccctggcg ctggagctga tcgcgccgcg actggtgacc agcgctccgg
     1201 aggcgctcct cgacgagctg cgcgaaacct tcgatcaggc gccagggcgg cctcgagaac
     1261 cgctggagcc ggccgttacc gtgcagcaga cgatgcgcct cagctacgac cagctcgacg
     1321 agcggcagca gcgggtcttc cgggtgttga gcatcatgta cgggcagtgg tcggtcgacg
     1381 acgtgctgcc gctgctgacg acggagttcg acgagctgga ggcggtcgcg ctgatcgagg
     1441 tgctggtcgc gaacagcctc atctcctcgc ccccctgccc ggccggggag gtgcgattca
     1501 ccgtcaaccc ggtcctgcag cacttcggca ggagcatgct agccgcgaaa ggggagcacc
     1561 agaccattgc ggaccgccat gccagccgaa tggtcgccct ggccgaggag gcggagccgg
     1621 agctcaatgg tccgaaccag caggagtggc tgacgcccct cgacgccctc tacgacgatt
     1681 tccggcacgc gcacgagcac ctccgagaga acggccggcg tgtcgatgcc cttcgcctgg
     1741 caaccgcgct ctggcgctac gcctacactc gcggtcacta ccggcagccg cggaggtgga
     1801 tcgaaacatc gctgggcgag atcagcggcc gtgatgccct tcgggcacgg tccctgacca
     1861 acggcgactt cgtcgagtca ggcgagatcc cggccaagat gctgcctgcg gtcgccgatc
     1921 tgcttgctga ggcgaacggc gatgccgaaa cacttgtcga gctgctgcag gaccgcaacg
     1981 tcgaggggct cgacgccggg gtggttgatc ggttggagac gtggctcctg gacaaagggt
     2041 ccatgacgcc ggcaaccccg ctcgggcatg acgagattcg ccggcgggcg ctgcgcgagg
     2101 cgtccggcga cctcgatgtc gggtcgatcg gtattctcga tatcgacgag ctactcgatt
     2161 acctgccgat cctgtagcac cgattccaca acaggcccgg cagcctcctg gaggcggtga
     2221 aaccgcagag tggaactcac accgactcgg acagggccag tcgtggagcc cggccctgcg
     2281 agtcaacaac gctcagcgcg ctgcgtggag tgcaatcgaa acgctacgcg ttcgctggcg
     2341 cctggtcgtc cgcgggcgtg aacgccgcgc acccgctgtc gggcgtgacc accagattgt
     2401 gctcagcatg gtcgggaaga ccgcacggct caaccagatc gtccggcgcc tcgtgggtgg
     2461 tgcggatctg gatcagctgc ggctggtcct gcatgttgtt ttcaccgaaa tgtgcgcaca
     2521 tgccgcattg tccgtcgtgt acctgaagca ttgccttctc ctttctctga cgcctctgtg
     2581 gagcgcccag ttctgccacg ccgacagccg agacgatcac agcccgccag gagcaatggt
     2641 cggaattcgt aaccgacaac cggggcgtgc ccgattcctg tcgactcgtg taagacctgt
     2701 gccttggccc tcggtgaccc ggtctgccaa gcgcgggccc tgctacactc accggagacc
     2761 gatcgacgtc gtccatccgt acaaggcacc gcacagcatg cccatcttcc gacgcgccag
     2821 accgggcgtc gctgagctgg cgagggacga gccgttcgcc ccgacgccga tctacatcac
     2881 cccacgcgtg cgcctcgtgc tcatcatcgc gagcttcgtg gtgctctacc tgctcgcgat
     2941 ggtcgccccg agcattccac gcctgctcat gctcgggggc acgctcgcgc tctccttccc
     3001 ggtccgcatg ctgaaccggt gggtgccgcg cagatgggcc atcgcgctcg ccgtgggctc
     3061 gaccctgttc ttcgccgtcc tgctcctgct catcctcgtg ccgttcgtga tcaccgaggt
     3121 gagcgggttc attcaggacc tgcccgagat cgtcgagggc gcgcaggacc ttacccgcga
     3181 tgtgatagcg gcagcgaacg aacgtggctg gatccgccac gacccggacg agatctacga
     3241 cacggtcgag tcgacagtga tcgatcgcgg gcaggcgctg atcgagacca cgctcgccgg
     3301 cgcactcgct gcgctgacgc ggacattcaa catcctgatc tcgaccttcg gcgtcttctt
     3361 catcgcgacc tatctgctga tcgacatgcc gcgcttccgg cagacgttcg tcaacagctt
     3421 cgcccagccc tatcggcggg acgccgagca cctctggcgg acgctgggcg agtcgctctc
     3481 gcgctatctc ggcgggctgg tggcctcgct gacgttgcag ggcatcgcgg ctgccgtcgg
     3541 gttgtggcta atcagtgtgc cgtatgcggt gctgctcggt atatggacgg cgatgacggc
     3601 ggtgttgccc tacatcggtg cctttctcgg agcgattccg gcgctcttca tcgcgttcac
     3661 ggtttcgtgg caggcggcag ccggggtggt ggtgctctac gtggtgatca accaggtcga
     3721 ggcaaatttc atcatccctc gcattcaggg gagcgccgtg cgggtccacc cgctgctgat
     3781 cttccttgcg gtgttgctcg gctccgagct gggtggtgcg ttcggggcga tcatcgccgt
     3841 gccgatgctg gcggtggtgc gggtgctggc cgagttcttc tgggtgcggc tgcgggtgcg
     3901 tggcgcgcag caaggcacgt tgctgtcggc gatcggcagc gaggatgtcg ctcccgaggc
     3961 ggggccgatg gtcctcgtcg aggacgacga gccggatcgg ccagtcgtcc gaatggacag
     4021 ctcatagtcc gatccgcacg tggcgagccg tctcgctggt ctcaattcgg atggtcgtca
     4081 cccagaccga ggcctgaacg gccgaggtga agtgccccgc gcgaagcacc agcgccgcag
     4141 gtgtcgttct cgttttaccc ggatttcgta tcaatgtagc gatatgatgg gtgggtggtc
     4201 tgctcagggg atccacgacg acccaaat
//
LOCUS       DASYWL010000240         3260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_161864, whole genome shotgun sequence.
ACCESSION   DASYWL010000240 DASYWL010000000
VERSION     DASYWL010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161864"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /gene="mutM"
                     /locus_tag="VGR22_08875"
     CDS             complement(<1..736)
                     /gene="mutM"
                     /locus_tag="VGR22_08875"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEV2128717.1"
                     /translation="MPELPEVETVRAIIEREASGRTIAGVTFRAFPGVVGLHTPEQFA
                     ALVTDRTIQTPVRRGKFLLLPLDDETALVVHLRMTGRLLVTAADVPPVRFEHLALHLD
                     NDADLRFSDQRKFGRVLHMLRDDAERSLSRLGPEPLSPHFNARYLAERANGRTAPVKS
                     FLLDQRHIAGLGNIYVDEALFLSRIHPLAPAGALTERDITSLVRSIRKVLRASIANQG
                     TTFATFENPYGERGNNAGALKVYGRAK"
     gene            808..1170
                     /locus_tag="VGR22_08880"
     CDS             808..1170
                     /locus_tag="VGR22_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEV2128718.1"
                     /translation="MAEKDCLFCKIVVGEIPAERIHETDDVLVFRDVTPQAPVHLLAV
                     PKRHIPSIASIGESDGELLGRLFGAANSAAEAELGDRGWRIATNHGGEAGQSVFHLHF
                     HIMGGAPLGPLATTEMKG"
     gene            1179..1679
                     /locus_tag="VGR22_08885"
     CDS             1179..1679
                     /locus_tag="VGR22_08885"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEV2128719.1"
                     /translation="MASLKERLTEDMKTAMREKDTARRDTVRLLVSEVKKKEIDLGHG
                     LHAAEELQLLQSQAKQRRDSIEQFRQGGREDLVAREQAQLEVIESYLPQQMTDEELDE
                     FIGEGIAQSGAQDPKDMGKLMGLLSKSSGGRVEGRRLSDAVRHRLTQFASDRGTIDKE
                     GGPVGG"
     gene            1669..2112
                     /gene="ybeY"
                     /locus_tag="VGR22_08890"
     CDS             1669..2112
                     /gene="ybeY"
                     /locus_tag="VGR22_08890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEV2128720.1"
                     /translation="MEVDVSVDWDVAGRAIEVTEGDAASLLEFALKQEGRGGAWTLAL
                     RFVGDAEMRSLHEQFMGERSSTDIMTFPYDGDDGEQGGDILISVDTADENARQQGAEL
                     ADELRFLMLHGLLHILVWDDHDERERQEMLDRQAALLAGWKATAA"
     gene            complement(2113..>3260)
                     /locus_tag="VGR22_08895"
     CDS             complement(2113..>3260)
                     /locus_tag="VGR22_08895"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEV2128721.1"
                     /translation="CAAAGIDLVRVTQVGDDLASILASLRLALEEADIVICTGGVGPT
                     DDDLTREAITSLAGETPEIDPNLLRDLKAFFANRGQEMPERNAKQAWVLPSGRVLPNP
                     IGTAPGWLVTIDRDDLNGTVIAMPGVPREMYRMWEEQALPAIQEQIGGHVIRSATLKT
                     LGIGESAAEDQIHDLIVRQQPVIATYAKDDGVHIRITVVDDSAHRAERQLEASISEVE
                     AILGRHIWGRDSERLPVVLLGAFERTKTTIAIAERGSGGALAGLLTSVPSEDQTHPLL
                     EARVLPLDFDLDQEAAIERARAMAARMRSETGAAIGLAIVVFGGFDERKVLHGTAAVA
                     IESERSGREVTFVGIRSAIEDAHRRAALHAADRLRQLLTAPESMETP"
BASE COUNT          581 a          988 c         1137 g          554 t
ORIGIN      
        1 gcttcgcacg accatagacc ttgagcgccc cggcattgtt gcctcgctcg ccgtatggat
       61 tctcaaaggt ggcgaacgtg gtcccctggt tggcgatcga ggctcgcagg actttccgga
      121 tcgagcgcac gaggctcgtg atatctcgct ctgtcagtgc acccgcgggc gcgagtgggt
      181 ggattcgcga caggaagagc gcctcgtcga cgtagatgtt gccaagaccg gcgatgtgtc
      241 gctggtcgag caggaatgac ttgaccggtg ccgtgcgacc gttggcacgc tcagccagat
      301 agcgtgcgtt gaagtgcgga ctgaggggct ccggaccgag tcgcgagagc gatcgctcgg
      361 cgtcgtcgcg caacatgtga agcacccggc cgaacttgcg ctggtcggag aagcgcagat
      421 ccgcgtcatt gtcgaggtgc agtgcaaggt gctcgaagcg gactggcggg acgtcggcgg
      481 cggtcaccag caggcggccg gtcattcgca ggtggaccac aagcgcggtc tcgtcatcga
      541 ggggcagcag caggaacttg ccgcgacgca ccggcgtctg gatggttcgg tcggtgacca
      601 gcgcggcgaa ctgctcgggt gtgtgcaggc caaccacgcc cgggaaggcg cggaaggtca
      661 cgccggcgat cgtgcgaccg cttgcctcgc gttcgatgat ggctcggacg gtctcgactt
      721 ccgggagttc tggcatactg gtttccgcaa tttgactcaa cgatgcgaca gagcccggta
      781 caccgggcga tcgagaggta ccgtagcatg gccgaaaagg actgcctgtt ctgcaagatc
      841 gtcgtcggcg agattccggc tgagcggatc cacgagaccg acgacgtgct cgtgttccgc
      901 gatgtcaccc cgcaggctcc tgtgcacctg ctcgctgtcc ctaagcggca tatcccctcg
      961 atcgcctcca taggcgagag cgatggggaa ttgctcggca ggctcttcgg cgccgccaac
     1021 agtgccgccg aggcggagct cggtgaccgc ggctggcgca ttgccacgaa tcacggcgga
     1081 gaggcgggac agagcgtctt tcacctgcat tttcacatca tgggcggagc cccgctgggg
     1141 ccgctcgcaa cgacagagat gaaaggatag gccccacgat ggcatcgctg aaggaacggc
     1201 tcaccgagga catgaagacg gcgatgcgcg agaaggacac ggcgcgccgc gacactgttc
     1261 ggctgctggt ttccgaggtg aagaagaaag agatcgacct cggacacggg ctgcatgctg
     1321 ccgaggaact gcaactcctg cagtcccagg caaagcagcg ccgcgactcg atcgagcagt
     1381 ttcggcaggg tgggcgcgag gacctggtcg cgagggagca ggcgcagctc gaagtcatcg
     1441 agtcctacct gccgcagcag atgaccgacg aagagctcga tgagtttatc ggggagggaa
     1501 tcgcgcagag cggcgcacag gacccgaagg acatgggcaa gctcatgggg ttgctgtcga
     1561 aatccagcgg tgggcgtgtc gagggtcgtc gtctctccga tgcggttcgg caccggctca
     1621 cgcagttcgc cagtgaccgc ggaacgatcg acaaggaggg cggacccgtt ggaggttgac
     1681 gtcagcgtcg actgggatgt cgccgggcga gcgatcgagg ttaccgaagg ggacgcggca
     1741 agcctgctgg aatttgcgct caaacaggag ggccgcggag gagcgtggac gttagcgctc
     1801 cgctttgtcg gcgacgccga aatgcgctcc cttcacgagc agttcatggg agagcgctca
     1861 tcgactgaca tcatgacgtt cccatacgat ggggatgacg gcgagcaggg cggcgatatc
     1921 ctcatctcgg tcgataccgc ggacgagaat gcccgccagc agggcgcgga gctcgctgat
     1981 gaacttcggt ttctcatgct gcatgggttg ctgcacatcc tcgtctggga cgaccatgac
     2041 gagcgcgagc ggcaggagat gctcgaccgg caggctgcgc tgctcgcggg ctggaaggcg
     2101 actgccgcct gattacggcg tctccatgct ttccggagcg gtcagcagct gtcgcaggcg
     2161 gtccgcggca tgcagggcgg cgcgccggtg ggcgtcctcg atggccgaac ggatgcccac
     2221 gaacgttacc tctcgaccgc tccgctcgga ctcgatcgcg accgcggctg tgccatgcaa
     2281 cacctttcgc tcgtcgaaac cgccgaatac gacgatggca aggccgatcg ctgcgccggt
     2341 ctcgctgcgc atgcgcgccg ccatcgcgcg agcccgctcg atggccgcct cctgatcgag
     2401 atcaaagtcg agcgggagca cgcgcgcctc aaggagggga tgggtctggt cttcgctcgg
     2461 cacggaggtg agcagtccgg ccagcgcgcc gccgctgcca cgctcggcga tcgcaatagt
     2521 cgtcttcgta cgctcgaagg cgcccagcag aacgacgggt agccgctccg agtcccgacc
     2581 ccaaatatgt cgtcctagga tcgcctcgac ctccgagatc gaagcttcga gctgacgctc
     2641 ggcgcgatgg gcggagtcgt ccacaacggt gatgcgaatg tgcacaccat cgtccttggc
     2701 ataggtggcg atcactggct gctgccggac gatcaggtcg tggatctgat cctcggcggc
     2761 cgattcgccg atgccgagcg tcttgagcgt tgccgaccgg atcacgtgcc cgccgatctg
     2821 ctcctgaatc gccggcaggg cctgctcctc ccacatgcgg tacatctctc gggggacgcc
     2881 cggcatggcg atgacggtgc cgttgagatc gtctcggtcg atagtgacga gccagccggg
     2941 cgcagtgccg atggggttgg ggagcacccg accgctcggc agcacccacg cctgcttggc
     3001 gttgcgctcg ggcatttcct ggccccggtt ggcgaagaag gccttgaggt cgcgcagaag
     3061 gttggggtcg atctcaggtg tctcgccagc gagggaagtg atcgcctctc gggtgaggtc
     3121 gtcgtcggtc ggaccgacac cgccggtgca gatgacgatg tcggcctctt cgagcgcgag
     3181 gcggagcgac gccaggatcg aagcgaggtc atcgccgacc tgtgtgaccc gaacaaggtc
     3241 gatgccggct gccgcacact
//
LOCUS       DASYWL010000241         5773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_180573, whole genome shotgun sequence.
ACCESSION   DASYWL010000241 DASYWL010000000
VERSION     DASYWL010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5773
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180573"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..1175
                     /locus_tag="VGR22_08900"
     CDS             234..1175
                     /locus_tag="VGR22_08900"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEV2128722.1"
                     /translation="MMDILHDLVFDYTLRNVAIGSMLLGIVSGVLGAFAVLRRQGLLG
                     DTLAHAALPGITIAFLLTGSRATIVLLLGAGIAGWLGTLLFLRIVQDTNLSEDTALGV
                     ILSTFFGFGVLLLTLIQNRNDATQAGLDRYLFGQAAALVASNVRTFAILGLLALTLLF
                     LFYKEFKLLSFDPAFAASIGLPTRTLDILLTSLLVLAVMIGLQTVGVVLMAAMVIGPG
                     AAARQWTDRLLTMLILAGFFGGLAGVTGAVLSVLGERLPTGPMIILALTVIVLVSLVL
                     APRHGFLPEYLRQRRNRRELKPPPEPVRHDLPEGGPA"
     gene            1172..2275
                     /locus_tag="VGR22_08905"
     CDS             1172..2275
                     /locus_tag="VGR22_08905"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV2128723.1"
                     /translation="MMSPTTAILLTAVLAAAACALPGTFLVLRRMSMMTDAISHAILP
                     GLVAGYFLAQGPNVLVGAVGATGAAILTVTAVEALQRSRYIDSGSAMGIIFPAMFALG
                     TVLVSRWFANVHLDTDAILYGNIEFSFFDRLVIGGRDLGPQSIWIMCTLLAINLLFLG
                     LFYKELKLTTFDPGMAAMLGFSPAVVHYALMLVLSVTTVGAFTAVGAILVVALVIVPA
                     ATAYLLTDRLIVMMGLSLAIGAAAGWSGYELAIRLDTSVSGAMVTMLGVFFLAALFFS
                     PSQGLIAKVLRQRNNARRFAVDLLLMHLWNHETTETESSESTLGHISAALRWPPDHAR
                     AVVTRAMSRGLVRQERERLTLTDTGREYAGELV"
     gene            complement(2281..3510)
                     /locus_tag="VGR22_08910"
     CDS             complement(2281..3510)
                     /locus_tag="VGR22_08910"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2128724.1"
                     /translation="MAIESAVGGFDTAAGRGFVLIAIAALSVGFAMAAQQNVVSNFFE
                     DSLGFGGPAFGYITAIREIPGFLLIFLTALFYRLSLPHLTAGALLLLAVGYGFFGFAT
                     SFWTVVPWVVISSMGYHTWLQTQAALGMSLTTEDKSGSVLGRISAITQGGALSAMAFI
                     FLAFHYDWLDFRGAFVVCGVMALIAALAIFRFPQLHNGEVQEVVPRRDPLVMRRDYRL
                     YYLLSLLDGARQQIFFSFGLWVLVDHFGLDVPAISAVMLVVAFGAMVLGPWIGRQIDV
                     RGERQMLGVVNVAYIVALLGYALVDQVSVAIACFVIYSFIFPFSAMGASIYLRKIAAT
                     RDIAPSLAMGLTMQHAAAVVVPIVTGFVLNYVGYQLPFLIASGFAVAAFFVTRMLDPK
                     TQKSAGRIREDTALATQ"
     gene            complement(3680..5293)
                     /locus_tag="VGR22_08915"
     CDS             complement(3680..5293)
                     /locus_tag="VGR22_08915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEV2128725.1"
                     /translation="MSHQPAASGHLDEQQYLSHSQIMGVMVGIMAGMFLAALDQSIVG
                     VALPRITSDLGGLEHLAWVVTAYLVASTATTPLWGKISDLYGRKRIFQTAIVIFLIGS
                     MLCGVAQDMPQLIGFRALQGIGGGGLMSIALAIIGDVIPPRERGRYQGYFGAVFGVSS
                     VAGPLLGGWLTDAISWRWIFYINLPIGILALFITSISLQMPLRRREHAIDYLGAGLVV
                     AAVSSLLLYLNWAGDHWGWGDPRALALLGAAIVLTVVFILVENRVREPIVPMRLFRNG
                     IFSVGNAFGFLSGLAMFGGVIFLPLYFQGVMGMSPTESGLAMVPMIGGLLFMSIISGL
                     LITQTGRYKIYPITGAALIIVGLLMLAQLEVETPYWQVGISAFVFGLGLGLTLQTVMV
                     AVQNAVEIRDMGVATSSLTFTRSLGGAIGTAVFGAVLNSRLATHLGEVMAGFDAGQAP
                     VNPEVTSNVEAMQALEEPARSIVLGAYTDALTDLFLFAIPFVAVALLVALFLREIPLR
                     SGMDRGGPTPRREADEERSETPEPVLTGQ"
BASE COUNT         1053 a         1984 c         1820 g          916 t
ORIGIN      
        1 cacgaagcgc tcggcagctt gttgccggtt cagtcgcatg gctgcaaaaa agcttgcatc
       61 ctgatgacca tcgcgaatga gatccttcga ctccggtgtt cggcctccac tcaggatgac
      121 agccatttcg gtgatttcga ggtttcgacg tggtcacgat cgtacgaagc gcgcctctct
      181 ttgggcggac ctaccggcca gtggcgctcc cgccagaaag gcggggtgac ggcatgatgg
      241 acattctgca cgacctggtc ttcgactaca ccctgcgcaa cgtcgcgatc ggctcgatgc
      301 tgctcggtat cgtcagcggc gtcctcggcg ccttcgccgt cctccgccgc cagggcctcc
      361 tcggcgatac cctcgcccac gccgccctcc ccggcatcac catcgccttc ctgctcaccg
      421 gcagccgcgc aaccatcgtc ctgctgctcg gtgcgggcat cgccggatgg ctcggcacgc
      481 tgctcttcct gcgcatcgtc caggacacga acctgagcga ggacaccgcg ctcggcgtga
      541 tcctcagcac cttctttggc ttcggagtcc tgctgctgac gcttatccag aaccggaacg
      601 acgctaccca ggccggcctc gaccgctatc tcttcggcca ggcagcggcg ctggtggcga
      661 gcaacgtgcg caccttcgcg atcctcgggc tactcgcgct gacgctcctg tttctcttct
      721 acaaggagtt caagctcctc tcgttcgatc ccgccttcgc cgccagcatt ggcctaccga
      781 cccgcacgct cgacatcctg ctcacctcgc tgctcgtgct ggcggtgatg atcggcctgc
      841 agacggtcgg cgtggtgctg atggccgcga tggtgatcgg ccccggcgcg gcggcacggc
      901 aatggaccga ccgcctgctc accatgctga tcctcgccgg attcttcggc gggctggcgg
      961 gcgtgaccgg cgcggtgctc agcgtactcg gcgagcgcct gccaacaggc ccgatgatca
     1021 tcctcgcact caccgtgatc gtgctggtct cgctcgtcct ggcgccacgc cacggcttcc
     1081 tgccggagta cctgcgccag cggcgcaacc gccgggaact gaagccgccg cccgagccgg
     1141 tccggcatga cctgccggaa ggaggcccgg catgatgagc ccgacgacgg ccatcctcct
     1201 caccgccgtg ctcgccgcgg ccgcctgcgc gctgcccggc acgttcctcg tgttgcggcg
     1261 gatgtcgatg atgaccgatg cgatcagcca cgccatcctg ccggggctgg tggccggcta
     1321 cttcctcgcg cagggaccga acgtgctcgt tggcgcggtc ggcgcgaccg gtgcggcgat
     1381 cctgaccgtt actgccgtcg aggcgctgca gcgatcgcgc tacatcgaca gcggctcggc
     1441 gatgggtatt atcttcccgg cgatgttcgc actcggcacc gtcctcgtct cgcgatggtt
     1501 cgccaacgtt cacctcgaca ccgacgcgat cctctacggc aacatcgagt tctccttctt
     1561 cgaccgcctt gtgatcggag gtcgtgacct cgggccgcag tcgatctgga tcatgtgcac
     1621 gctgctggcc atcaacctgc tcttcctcgg gctcttctac aaggagctca agctgaccac
     1681 cttcgacccc ggcatggcgg ccatgctcgg cttctcgccg gcggtcgtgc actacgcgct
     1741 gatgctggtg ctctccgtga cgacggtcgg cgccttcacc gcggtcggcg cgatcctggt
     1801 cgttgcgctg gtgatcgtgc cggcggcgac ggcctacctg ctgaccgacc ggctgatcgt
     1861 gatgatgggc ctctcgctgg caatcggcgc ggcggcgggc tggagcggct acgagctggc
     1921 gatccggctc gacacgtcgg tctccggcgc gatggtaacg atgctcggcg tcttcttcct
     1981 ggcggcgctg ttcttctcgc cgtcgcaggg actgattgcg aaggtgctgc gacagcgcaa
     2041 caacgcccgg cggttcgccg tcgacctgct gctgatgcat ctgtggaacc acgagaccac
     2101 ggaaacggag agcagcgaga gcacgctcgg gcatatcagc gccgcgctgc gctggccacc
     2161 ggaccacgcg cgggcggtgg tcacccgagc gatgagccgg ggcctggtcc ggcaggaacg
     2221 cgagcgcctc accctcaccg acaccggccg ggaatatgct ggcgagctgg tttgaggcaa
     2281 tcattgtgtg gcgagggcgg tgtcctccct gatccggccg gccgatttct gcgttttcgg
     2341 gtcgagcatt cgcgtgacga agaacgcagc caccgcaaac ccgctcgcga tcaggaacgg
     2401 gagctggtag ccgacgtagt tcagcacgaa gccggtcaca attggcacga ccaccgccgc
     2461 ggcgtgctgc atggtcagcc ccatcgcgag ggacggcgcg atatctcgcg tcgccgcgat
     2521 cttgcgcagg tagatcgatg cgcccatcgc cgagaacggg aagatgaacg agtagatgac
     2581 aaagcaggcg atcgccacac tgacctggtc gaccaacgcg tagcccagca gcgcgacgat
     2641 gtaggccacg ttcaccacgc cgagcatctg ccgctcgccg cgcacgtcga tctgccgccc
     2701 gatccacggg cccagcacca ttgctccgaa ggcaaccacc agcatcaccg ccgagatcgc
     2761 cggcacgtca aggccgaagt ggtcgaccag cacccagagg ccgaacgaga agaagatctg
     2821 ctggcgggcg ccgtcgagca gactcaggag gtagtagaga cggtagtcgc gccgcatcac
     2881 gagcggatcg cgcctgggca ccacctcctg cacctcgccg ttgtgcagct gtgggaagcg
     2941 aaagatcgcc agtgccgcga tcaatgccat cacgccgcag acgacaaagg cgcccctgaa
     3001 gtcgagccag tcatagtgga aggcgaggaa gatgaacgcc atcgcgctca gcgcgccgcc
     3061 ctgggtgatc gctgagatgc gcccgagcac cgagccggac ttgtcttcgg tggtcaggct
     3121 catgccaagc gccgcctgcg tctgcagcca ggtgtgatac cccatcgagg agatgaccac
     3181 ccacggcacc acggtccaga acgaggtggc gaacccgaag aagccgtagc cgacggccag
     3241 caacagcaac gccccggcgg tcaggtgcgg cagcgagagc cggtagaaaa gcgccgtgag
     3301 gaagatcagc aggaagccgg ggatctcgcg gatggcggtg atgtagccga acgcagggcc
     3361 gccgaacccg aggctgtcct cgaagaagtt ggagacgacg ttctgctggg cagccatcgc
     3421 gaacccgacc gagagcgccg cgatggcgat gagcacgaag ccgcgtcccg ccgcggtgtc
     3481 gaagcccccg accgccgatt cgatcgccac acgccgccgt tgtaccaacc cgctgccaac
     3541 cacgcctcgt ccccaatcct cgtgtcgccc caacgctgtc gctgtccggg atacattccg
     3601 cccggcagta tcggtggaaa cagactgtgc cgcaaacggc aggaaagcgc tgcggtacag
     3661 ctaccgagcc gcaaacgccc tattgaccgg taagcaccgg ctccggagtc tccgatcgct
     3721 cctcgtcggc ctcccggcgc ggcgtcggcc cgccccgatc catgcccgag cgcagcggga
     3781 tctcccgcag gaagagcgcg accagcagcg ccaccgcgac gaacgggatc gcgaagagga
     3841 agaggtcggt cagggcatcg gtgtatgccc ccagcacgat cgagcgcgcc ggctcctcga
     3901 gcgcctgcat ggcctcgaca ttgctggtca cctccgggtt gactggcgcc tggcctgcat
     3961 cgaatcccgc cataacctca ccgaggtggg tcgccagccg gctgttgagc accgccccga
     4021 agaccgccgt gccgatcgca cctcccagcg agcgggtgaa cgtgagcgag ctagtcgcca
     4081 cccccatgtc gcggatctcg acggcgttct gcaccgccac catcaccgtc tgcaacgtca
     4141 gcccgagccc gagtccaaag acaaaggcag agatcccgac ctgccagtag ggcgtctcca
     4201 cctcgagttg cgcaagcatc agcagcccga cgatgatcag cgccgcgccg gtgatcgggt
     4261 agatcttgta ccgcccggtt tgggtgatca acaggccgga gatgatcgac atgaagagca
     4321 ggccgccgat catcggaacc atcgccaagc cggactccgt cggcgacatc cccatcacac
     4381 cctggaagta aagcggcagg aagatcaccc cgccaaacat cgccaggccc gacaggaacc
     4441 cgaaggcgtt gccgacggag aagatcccgt tgcggaagag ccgcatcggc acgatcggct
     4501 ccctcacacg gttctcgacg agaatgaaga cgactgtcag cacgatcgcc gcacccagca
     4561 gcgccagcgc acgcgggtcg ccccagcccc agtggtcacc ggcccagttg aggtagagca
     4621 gcagcgacga caccgccgcc accaccaacc cggcaccgag gtaatcgatc gcgtgctcgc
     4681 ggcgacgcag cggcatctgc agcgagatcg acgtgatgaa cagcgcgagg atgccgatcg
     4741 gcaggttgat gtagaagatc cagcgccagc tgatcgcatc ggtgagccac ccacccagca
     4801 gcggtccggc aacgctcgac acgccgaaca cggccccgaa gtagccctgg tagcggccgc
     4861 gctcccgcgg cgggatgaca tcaccgatga tcgccagcgc gatcgacatc aggccaccgc
     4921 cgccgatgcc ctgcagcgcc cggaaaccga tcagctgggg catgtcctgg gcaacaccgc
     4981 acagcatcga cccgatcagg aagatcacga tcgccgtctg gaagatgcgc ttgcgcccgt
     5041 agaggtcgga gatcttgccc cagagcggtg tggtcgcggt cgacgcgacg aggtaggcgg
     5101 taacgaccca ggcgagatgt tccaggccgc cgaggtcgct ggtgatgcgg ggcagcgcga
     5161 cgccgacgat gctctgatcg agcgcggcga gaaacatgcc ggccatgatg ccgaccatca
     5221 cgcccatgat ctgggaatgg gagaggtact gctgctcgtc gagatgcccc gacgcggcgg
     5281 gttggtggct cacgtgatgg actccggaag agaaggattg aaaaggcgcc gccggatggt
     5341 gcggaacccc gtgttgacca gccgatcagc gcacatcgct gtagcgaggc gcgaaaccac
     5401 cgtcaacgag ctgggcgttc gcaaggacac tgcctcaacg atacgctcct cctgttcaga
     5461 cgggtggagt gttgcgatgg atgcaatgct cgatctccag aaccacacga aaccggtatc
     5521 atcctgagct ggttgcgacg ccgtcggtgc agcagtcgtg ccgaatcctg acgtcgatac
     5581 aaccatggct gggaatccaa cgcgctgagg aacaagacgg accgcccctg ccacatggca
     5641 ggggcgggga tccttcgact ccggcccgtc gggcctccgc tcaggatgac atcacgttca
     5701 cagatctctc cgtggcttcc atccataccc tacgctaccc caactcggat gcggtggtag
     5761 ccactggcac cgg
//
LOCUS       DASYWL010000242         5573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_555931, whole genome shotgun sequence.
ACCESSION   DASYWL010000242 DASYWL010000000
VERSION     DASYWL010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555931"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VGR22_08920"
     CDS             complement(<1..624)
                     /locus_tag="VGR22_08920"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV2128726.1"
                     /translation="MPESFDRELEERLVRYARIHTTSEEASETTPSTERQYDLLRLLA
                     DELGEMGAEEITLTDYGAVLATIPATIGTEVSAIAFLAHVDTTESYSGEGVEPIVHRS
                     WDGSDIVLPDNPELVLSAERLPYLGTKTGGDIITASGTTLLGADDKAGVAIIMALARH
                     LLASADIPHGPVRVCFTPDEEIGRGVHENLPQDLRADVAYTLDGALYP"
     gene            complement(653..2188)
                     /locus_tag="VGR22_08925"
     CDS             complement(653..2188)
                     /locus_tag="VGR22_08925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HEV2128727.1"
                     /translation="MIAPDERLPWGQSFAMGAQHVLAMFGATVVAPLIMGFDANVAIF
                     FSGIGTLIFFVMTGGRIPSYLGSSFAFIAPVAAVTGSAATGDFNAGDIPEALGGIIAA
                     GLLYTIIGAVVDYTGSGWIDALMPPLVTGSVVAIIGLNLAGAARGLVDQNGWLALITI
                     LAIMGIALLTKGILGRLPILLGTVVGYLAALILGGTTAAGRDLGFATISGVDFQPVRD
                     AAWIGFPDFVTPSFEWNAISIIAPVAIVLVAENTGHIKAVSAMTHRDLMPWLGKGFMG
                     DGVATMVAGAGGGTGVTTYAENIGVMAVTRVYSTVVFVIAAGVAILLGLSPKFGALIN
                     SIPAGVLGGVSTVLFGLIAITGARIWVENRVDFSRGVNLFVASVAIVVGAANYTVHFG
                     NFTFEGITLGTFGAIILYQLFRPAEPDAERPVPDRPEPEYEDADYNVPGASYVDDRRT
                     RVARGGLQGGRQRDEDQGFEEHLPDLPPGDRRPGDGTAQGHRRSSAESGRPRRPRPRV
                     DEE"
     gene            complement(2540..3412)
                     /locus_tag="VGR22_08930"
     CDS             complement(2540..3412)
                     /locus_tag="VGR22_08930"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEV2128728.1"
                     /translation="MPVKLAISSYSFHRFGGGPEGDDARTIEQMTDGCADYGVSGLEV
                     LMQHFERNDATSPNRLHELRQYAAIRGITPVTVAANNNPVQPDPGQREVELQKVRDAV
                     DMAQAIGAPFVRAMGGRWHTTSSFQEMLEHGGEEPPAEGYTEDDGYDWAISALKEGAA
                     YAGERGVTLVLENHWGLTGTAGGCVRIHDAVDSPWLKYVLDLGNFFRRPDQYAEMARF
                     FGDLAVVHAKVYLGGGMLVEPEIDYERVAGMLEEIGYSGYISMEFEGKAHPDDGIPAG
                     LEQLRSAFGLPPGA"
     gene            3778..4758
                     /locus_tag="VGR22_08935"
     CDS             3778..4758
                     /locus_tag="VGR22_08935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV2128729.1"
                     /translation="MRRLRILTWPIHGSYFNTLTRLDHDWLLPVKPGGPEGYGGRGDN
                     PFPPSIQDVPAESVLDTDLDLVLFQSDQNLDQDALILSPSQRRLPRIYLEHNTPFPHP
                     FASRHPFADSHGLLVHVTRYNKLMWDNGQTATRTIEHSVAIDPTVTWEGTRPEGITAI
                     NSMPRRGRKVGLDLLLEARRQVPIQLAGFGNDGLDGLGDIPYSRLHRTMAEYRFLFSP
                     CRYTSLPLAVIEALTIGMPVVALATTELPDVIRNGVHGYLSNDLDELIAGMRRLIDDP
                     DHARELGANARELAAQRFGLDRFARDWDAAFAQAIDLAASSGSPAPTAVR"
     gene            4801..5547
                     /locus_tag="VGR22_08940"
     CDS             4801..5547
                     /locus_tag="VGR22_08940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV2128730.1"
                     /translation="MTDETSIRAAVALVTGAGSGLGEATARAFADAGGTVVCIDRNAD
                     ATARLLSELPRHAGEHLALACDVREADQVQEAADETVQRLGRIDILVNSAGIDHTHWV
                     EELTVQQWDDVLDVNLRGPFLFAKAAWPQMRQQGRGHIFNIASTSSLRAASGASAYSA
                     SKFGLLGLGRALNLEGRTDNIRVTTVIPGGMRTHFFDRFADQDIPMPDPANLQDPANV
                     ARAILFAAQMPYGSDVQELVITSPKETAWP"
BASE COUNT          985 a         1942 c         1738 g          908 t
ORIGIN      
        1 ggggtacaag gcgccgtcaa gcgtgtacgc gacatcggcg cgaaggtcct gcggcaagtt
       61 ctcgtgtacg ccccgtccga tctcctcgtc aggcgtgaag cagacacgta cgggcccgtg
      121 cgggatgtcg gcgctcgcca ggaggtgtcg ggcgagcgcc atgatgatcg cgacgccggc
      181 cttgtcgtcg gcaccgagca acgtcgtccc gctcgcggtg atgatgtcgc cgccggtctt
      241 cgtccccaga tacggcagcc gttcggccga gagcacgagc tccgggttgt ccggcagcac
      301 aatgtcacta ccatcccaac tccggtggac gatcggctcc acgccctcgc cgctgtacga
      361 ctccgtcgtg tcgacgtgcg cgaggaaggc gatggccgac acctcggttc cgatggtcgc
      421 cgggatcgtg gccaagacgg cgccgtagtc tgtcagggtg atctcctccg cgcccatctc
      481 gccgagttcg tcagcaagca ggcgcagcag gtcgtactgc cgctcggtgc tcggtgtcgt
      541 ctccgacgcc tcctcacttg tggtgtggat gcgagcatag cggaccagcc gctcctcgag
      601 ttcccggtcg aatgactccg gcatgaatga tctcctcgca accctggggc cgctactcct
      661 cgtccacccg cgggcgtggc cgccgtggtc gccccgattc tgcggacgac cggcggtggc
      721 cctgcgcggt accgtcgcct ggccggcgat cgcccggcgg caggtcgggc aggtgctcct
      781 cgaaaccctg gtcctcgtcg cgctgccgac cgccctggag cccgcctctc gccactcgcg
      841 tgcgccggtc atcgacatag gacgcacccg gaacgttgta gtcggcgtcc tcatactctg
      901 gctcgggacg gtcgggcacc ggacgctcgg catccggctc ggccggccgg aagagctggt
      961 agaggatgat cgcgccgaag gtgcccagcg tgatgccctc aaaggtgaaa ttgccgaagt
     1021 gcaccgtgta gttagctgcg ccgacgacga tcgccacgga tgcgacgaag aggttcacgc
     1081 cacggctgaa gtcgacccgg ttctccaccc agatccgtgc accggtgatg gcgatcagtc
     1141 cgaagagcac tgtcgagaca ccgcccagca cgccggcggg gatcgagttg atgagcgcgc
     1201 cgaacttcgg tgacagcccg agcaggatcg ccacgccggc ggcaatcacg aagacgaccg
     1261 tcgagtagac ccgcgtgacg gccatcacgc cgatgttctc ggcataggtc gtcacaccag
     1321 taccaccccc ggcaccggcg accatcgtcg cgacgccatc acccatgaag cccttgccga
     1381 gccacggcat caggtcgcgg tgggtcatcg ccgacacggc cttgatgtgc ccggtgttct
     1441 cggcaacgag cacgatcgcc accggcgcga tgatcgagat ggcgttccat tcgaatgacg
     1501 gcgtgacgaa atcggggaac ccgatccagg cggcgtcacg caccggctga aaatcgacgc
     1561 cgctgatggt tgcgaaaccg agatcgcgcc ccgcggcggt cgttccaccg aggatgagcg
     1621 ccgccaggta cccgaccacg gtgccgagca gaatcggcaa ccgtccaagg atgcccttgg
     1681 tcagaagcgc gatccccatg atggcgagaa tcgtgatgag ggccagccag ccattctggt
     1741 cgaccagccc cctggccgca ccggcgaggt tgagcccgat gatggccaca accgagccgg
     1801 tcaccagagg cggcataagc gcatcgatcc agcccgatcc agtgtagtca acgaccgcgc
     1861 cgatgatcgt gtagagcagg ccggccgcga tgatgccgcc gagcgcctcc ggtatatcgc
     1921 ccgcgttgaa gtctcccgtc gcggccgagc cagtcaccgc cgccaccggt gcgatgaagg
     1981 cgaagctgga gccgagatag ctcgggatgc ggccgccggt catcacgaag aagatgagcg
     2041 taccgatgcc ggagaagaag atcgcgacgt tggcgtcgaa gcccatgatg agcggcgcca
     2101 cgacggtcgc gccgaacatc gccagcacat gctgggcacc catggcgaac gactgtcccc
     2161 acggcagccg ctcgtccggt gcgatcaccc cacctcgtcg ctctcgccaa gaaaacatgg
     2221 atcccaaacc ggagagatcc ggcccgttgc gtccagatgc cacagggtag agccctcccc
     2281 cgaacagttc gcgtggccca ctcacatccg cgtgacgtgc ccgccacgcc ggcatggcag
     2341 agacctttcg gatgctccgg tcgcgatgtt tctggttccc tattgtggcg tccgtatgcc
     2401 tgtcctgcaa ccgctttgcc agccgcccac cctcgaacgg aagcgctcat cgccgctcac
     2461 tgccccgcca acggatgcca tccttcgctc agcacgatac ggctcatccg gctgcgcatg
     2521 caacgaaaac cgacccggct tacgccccgg gtggcagccc gaacgccgag cgcagttgct
     2581 cgaggccggc cgggatgccg tcgtccgggt gcgccttgcc ctcgaactcc atcgagatat
     2641 agccgctgta gccgatctct tccagcatcc cggcaacccg ctcgtagtcg atctccggct
     2701 ccacgagcat gccgccgccg agatagacct tcgcgtgcac gaccgcgagg tccccgaaga
     2761 accgcgccat ctcggcatac tgatcgggac gacggaagaa gttgccgaga tcgagcacat
     2821 acttcagcca cggtgagtcg acggcgtcgt ggatacgcac gcagccgcct gcggtgccgg
     2881 tcagtcccca gtgattttcg agtacgagcg tgacgccgcg ctcacccgcg tatgccgcac
     2941 cctccttcag tgccgagatc gcccagtcgt acccgtcatc ctcggtgtag ccctcggccg
     3001 gcggctcctc gccgccatgc tccagcatct cctgaaacga cgaagtggtg tgccagcggc
     3061 cgcccatcgc gcgaacgaag ggcgcgccaa tcgcctgcgc catgtcgacg gcatcccgca
     3121 ccttctgcag ctcgacctcg cgctgcccgg ggtccggctg aacggggttg ttgttcgccg
     3181 caaccgtcac cggtgtgatg ccccgaatcg cggcgtactg gcgcagctcg tggaggcggt
     3241 tgggcgaggt cgcgtcgttg cgctcgaagt gctgcatgag cacctccagg ccgctgacgc
     3301 cgtagtcggc acaaccgtcg gtcatctgct cgatggtccg ggcgtcatcg ccctccggac
     3361 cgccgccgaa gcggtggaag gagtaggacg agatcgcgag cttcacaggc atggccggta
     3421 gtttccttct cgctgaatct acaggtgaag ccgagtcacg tctggcactc cccacaagag
     3481 gtgaggaaac cagcagggct gaccactgtc aatccttgct ccaaccagag gcctgccgcc
     3541 gcgcgcattc actttggctc ctggcgacca gcgtgaagga tcgtgcaact ccaacccgca
     3601 gggaccgtcg cttcgtgtac gtccggcgcc tcgcgtagga tgttgctcag caggatggcg
     3661 tcgactgcac gatccaactt gccggtcgag ccactaggca tcttctcgcc ctgatcacgt
     3721 gagcaacggt cgccacgaca aggacatcgc gaccgacacg cacaggagtg caaacccatg
     3781 cgacggctcc ggatactcac ctggccaatt cacggcagct acttcaatac gctcacacgc
     3841 ctcgaccatg actggttgct gccggtgaaa ccgggcggcc ccgaggggta cggtggtcgc
     3901 ggcgacaacc ccttcccacc gtcgatccag gatgtgccgg ccgaatcggt gcttgacacg
     3961 gacctcgacc tcgtgctctt tcaatccgac cagaatctgg accaggacgc cctcatcctg
     4021 agcccgtcgc agcgtcgcct gccgcggatc tacctggagc acaacacgcc cttcccgcat
     4081 ccattcgcga gccgtcatcc gttcgccgat tcccacgggc tcttggtcca cgtcactcgc
     4141 tacaacaagt tgatgtggga caacgggcaa accgccaccc gcaccatcga gcacagcgtg
     4201 gcaatcgatc cgaccgtcac ctgggagggc acccggccag aggggatcac ggcgatcaac
     4261 agcatgcccc ggcgcggtcg caaggtcgga ctcgatctcc tcctggaggc gcggcggcag
     4321 gtccccatcc agcttgccgg gttcgggaac gacggcctgg acgggctcgg cgacatcccc
     4381 tactcccgcc tgcaccgcac gatggccgag taccgcttcc tgttcagccc ctgtcggtac
     4441 accagccttc ccctggccgt catcgaggcc ctgaccatcg gcatgccggt cgtcgcgctg
     4501 gcgaccaccg agctgccgga cgtgatccga aacggcgtgc atgggtacct ctcgaacgac
     4561 ctggatgagc tcatcgcggg gatgcgtcgc ctgatcgacg atcctgacca tgccagggag
     4621 ctcggagcca acgcgcggga actcgccgcc caacgcttcg gcctcgatcg gttcgctcgg
     4681 gactgggatg cggccttcgc ccaggcgatc gacctcgccg cctcatccgg cagcccagct
     4741 ccgactgctg tacggtagcg gtgacgatcc gagaccgaac gcgtcgggag gaaacacatc
     4801 gtgacggatg agacatcgat tcgggccgcc gttgctctgg tgacgggcgc cggcagtggc
     4861 cttggagaag cgacggcacg agcatttgcg gacgccgggg gcacggtggt ctgcatcgac
     4921 cggaacgcgg acgccaccgc acgactcctg tcggagctgc cgcgacatgc cggcgagcac
     4981 ctcgcgctag catgcgacgt gcgcgaggcc gatcaggtcc aggaggcggc cgacgagacg
     5041 gttcagcggc tgggccgcat cgacatcctg gtcaactcgg cgggcattga tcacacccac
     5101 tgggtggagg agttgactgt ccagcaatgg gatgacgtcc tcgacgtcaa cctccggggg
     5161 ccgttcctgt tcgccaaggc tgcctggccg cagatgcgac agcagggccg tggccatatc
     5221 ttcaacatcg cctcaacgtc atccctgcgg gcagccagcg gcgccagcgc gtactccgca
     5281 tccaagttcg ggctgctggg actgggtcgg gcgctgaacc tggaggggcg cacggacaat
     5341 atcagggtca cgaccgtgat tccgggcggc atgcgcacgc acttcttcga tcgcttcgcc
     5401 gatcaggaca tcccgatgcc cgacccagcc aacctgcagg atccggcgaa cgtcgcccgc
     5461 gcgatcctgt tcgcggccca aatgccctat ggctcggacg tgcaggagct ggtgattacc
     5521 tctcccaagg agactgcctg gccgtgattc acatgccacc catccgcctt cac
//
LOCUS       DASYWL010000243         3640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_274269, whole genome shotgun sequence.
ACCESSION   DASYWL010000243 DASYWL010000000
VERSION     DASYWL010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274269"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..396
                     /locus_tag="VGR22_08945"
     CDS             271..396
                     /locus_tag="VGR22_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128731.1"
                     /translation="MDIFEEMGGCVVIGIVAVVVLLLVVCVAVVFFGFAITEVFE"
     gene            complement(544..1131)
                     /locus_tag="VGR22_08950"
     CDS             complement(544..1131)
                     /locus_tag="VGR22_08950"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV2128732.1"
                     /translation="MNRSFLPSRLTLTRRGALARLGAGGIAVGLATRAHLSLAQESTP
                     PVSPGNIPPELQSWVDGWEATNAEQIASTYAEDAVVEVVPFGTVLRGREAIEQYYAAY
                     FGAFDAPSARITGLFTAGDQAAAEWTFQGGYEGQLPGLPPGEGQQVSLRGVNVMEVSD
                     GQIVVERIYTDLSGLLAQLGIEGAIPGTPAATPTG"
     gene            complement(1338..1799)
                     /locus_tag="VGR22_08955"
     CDS             complement(1338..1799)
                     /locus_tag="VGR22_08955"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV2128733.1"
                     /translation="MEYQRSRRIRVTPDSVFAFVTDVSNLPTYVPTVQSVETLTDGRI
                     RVHGNTGGTSFEADLWTHVDHNQRGIEWGTDERDRHGWMTVTAADDDADMTEVVAHVS
                     WPPHVNQDAAPLAGERAEEPDPIEDALEAALDSLRNILEGTGGKERPESVS"
     gene            complement(2034..2660)
                     /locus_tag="VGR22_08960"
     CDS             complement(2034..2660)
                     /locus_tag="VGR22_08960"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEV2128734.1"
                     /translation="MSKIHFYEPATGHGLPHDPFKAIVAPRPIGWVSTLSTDGVLNLA
                     PYSFFNAVSATPPIIAFSSSGRKDSLVNVEATGEFIWNMATRALAEEMNASSARVPPE
                     VDEFTLAGLETAPSRLVASPRVAASPASLECRLLQIVELHDLDGQPVGSSVVLGQVVG
                     VHIDRTFLRHGLFDTGAARPIMRAGYRDAYVEATPETMFKMMRPDPQD"
     gene            2852..3178
                     /locus_tag="VGR22_08965"
     CDS             2852..3178
                     /locus_tag="VGR22_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128735.1"
                     /translation="MTTTVLRRFALILLLAFSPGFAVLTPLAGSAQGSARNCQVTGAW
                     HLVLDEGAIDVICDLRLEADNADDGAEDLPIGDEVARCEQIAVDAGIKTWTTTTSSPP
                     AIVTTI"
     gene            3187..3426
                     /locus_tag="VGR22_08970"
     CDS             3187..3426
                     /locus_tag="VGR22_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128736.1"
                     /translation="MIDDDDLICGCDGSNVLGSLDGGDVFLGGEGDDVVELVQGGIVN
                     GGEGGDLVVILDGGTFSGGAGEDAVRDLRDAIFNE"
BASE COUNT          636 a         1107 c         1239 g          658 t
ORIGIN      
        1 cggtcgagag cgaggagggc cgcggcagca ccttcaccgt tcgcctgccc aaccagatgt
       61 gctgatcagc accctctgcg agcgctcgct gcattcatca tcgtgaacgg tgatgcgagc
      121 attctgcgac tttttgcagg cgatgtcctc gaggtcatgg tcctccgccg aggaacaccc
      181 agtgacctgc cacacttgca tgccgatcac gcatgtttca ggtaccatca ggacttgcct
      241 gtgttgcagg tatgaaggca gggatgaggc atggatatct tcgaggaaat gggcggctgc
      301 gtggtgatcg gcatcgttgc cgtcgtcgtc ctgctgctcg tggtctgcgt cgccgtggtc
      361 ttcttcggct tcgcgatcac cgaagtattc gagtagccca gcggtatcgg gtcatggtcg
      421 agcgccggcc caggacggca ggtggtgagt gtggcaagac ccccggacta cggtccgggg
      481 ggcttgatgc tggctggatc ggccgattgc ggggctggat gttggccacc catcgtgccg
      541 ctgctacccg gtgggcgttg cggcgggtgt gccggggatc gccccttcga taccaagctg
      601 ggcaagcagc cccgagaggt cggtgtagat gcgctcgacc acgatctggc cgtcgctcac
      661 ctccatgaca ttgacgccgc gcaacgaaac ctgctgccct tcgcccggcg gaaggccggg
      721 tagctgaccc tcgtatccgc cctggaaggt ccactctgcg gctgcctggt cgcctgcggt
      781 gaagagcccg gtgatcctcg cgctcggcgc gtcgaacgcg ccgaagtaag ccgcataata
      841 ctgctcgatc gcctcgcgtc cacgcagcac ggtgccgaag ggcaccacct cgaccacggc
      901 atcctcagcg taggtcgacg cgatctgctc ggcgttggtg gcctcccagc cgtctaccca
      961 agattggagc tctggcggga tgttgcctgg tgagaccggc ggagtgctct cctgcgcgag
     1021 ggagagatgg gcgcgggtgg cgaggccaac tgcgatgcca ccggcaccga gacgggccag
     1081 cgcgccacgg cgcgtgaggg tgagtcttga cgggagaaac gagcgattca tcgatacctc
     1141 cttgtcgtac gacgacgcgc ggacacggac gctgacatgg tgcgtccgca tgctctctcg
     1201 gtgccagacc tgatccaaca gtaccaagag ttatgtgcga cgtaaagctg ctcgtcggac
     1261 cggatggcca aagaggctcg cgtgtccaga tgctggcgac gtcccggcgg acagactcgg
     1321 ccctgccgta cgcgaggtca ggagacgctt tcaggccgtt ccttgccgcc agtcccttcg
     1381 agaatgttgc gcagcgaatc gagcgccgcc tccagagcgt cctcgatcgg gtccggctcc
     1441 tcggcgcgct cacccgccag cggagcggcg tcctggttca cgtgcggggg ccacgacaca
     1501 tgggcgacca cctctgtcat gtccgcatcg tcatccgcag cagtgaccgt catccagcca
     1561 tggcgatcgc gctcgtcggt gccccactca atgccgcgct ggttgtggtc gacgtgggtc
     1621 caaagatccg cctcaaagga ggtgcctccg gtgttgccgt gcacccggat acgaccgtcc
     1681 gtcagggtct cgacggactg gaccgttggg acatatgtcg gaaggttgct gacgtcggtg
     1741 acgaaggcga agacgctgtc cggggtaacg cggatgcggc gggatcgctg atactccaag
     1801 accctatcct ccctgcaacg cattggctat cgttagcgca ggggagccat gccccggcgc
     1861 cgctcttggg acgtggtcgc atagggggtg ccagcacagc acaatcaccg atctgcatgc
     1921 cttcaagccc tggtcgttct cgttaatggt gggcggtggt tgaggacggc aggcacggcg
     1981 ccctggggga gggcgagagt accgaccgcc ggcgcgcgga ccagattcgc cggttaatcc
     2041 tgcgggtccg gccgcatcat cttgaacatc gtctccggag tcgcctcgac gtaggcgtcg
     2101 cgatacccgg cgcgcataat cgggcgcgcg gcaccggtat cgaacaggcc gtgccgaaga
     2161 aatgtgcggt cgatgtggac gccgacgacc tggccgagca ccacgctcga gccgaccggc
     2221 tggccgtcga ggtcgtgcag ctcgacgatc tgcagcaggc ggcactccag tgacgccggg
     2281 ctggcggcca cgcggggcga cgcaacgagg cggctgggcg cggtttcgag cccggcgagt
     2341 gtgaactcgt cgacctctgg cggcacgcgt gccgacgagg cgttcatctc ctccgccaaa
     2401 gcgcgagtgg ccatgttcca gatgaactcg ccggtcgcct cgacgttgac gagactgtcc
     2461 ttgcggccgc tcgacgagaa ggcgatgatc ggcggcgtgg cactcaccgc attgaagaaa
     2521 ctgtagggcg cgaggttgag aaccccgtcg gtgctcagcg tcgaaaccca cccgatcggg
     2581 cgaggggcga cgatcgcctt gaacgggtca tgtggcaggc cgtggccggt ggctggctcg
     2641 tagaagtgga tcttgctcat ggcagtgagc atagacgagg atcggggacg gcacaaccgt
     2701 cgaatggagg tgcgccagcg tgcctgcaat tcgattcggg gtggaacctg tgttcagacc
     2761 cgctggaccg aaacatgctg ggcagtcgtc ttgagcaagg acgagaacaa tcaggagaat
     2821 tgcataccgg aacggaggat aaatcacgct ggtgacaacg acggtactcc gacgcttcgc
     2881 gctgattctg ctgctggcgt tctctccagg gttcgcggtg ctcacacccc tcgccgggtc
     2941 ggcgcaggga tccgccagga actgccaggt gacaggggct tggcacctcg tcctggacga
     3001 gggagccatc gacgtcatct gcgatctgag gctcgaagcg gacaacgccg atgacggcgc
     3061 cgaggatctg cctattggcg acgaagtcgc gcgctgcgag cagatcgccg tcgatgccgg
     3121 gatcaagacc tggacgacta caacatcatc accgccagcg atcgtgacga cgatctgaac
     3181 aatattgtga tcgacgacga cgatctgatc tgcggctgcg acggcagcaa tgtcctcggg
     3241 tctctcgatg ggggcgatgt tttcctgggc ggtgagggcg acgatgtcgt ggagctcgta
     3301 cagggcggca ttgtcaacgg gggcgagggc ggcgatctcg ttgtcatttt ggacggtggt
     3361 accttcagcg gcggagctgg cgaagacgcc gtgcgtgacc tgcgcgacgc catcttcaac
     3421 gagtagcgct gcttggctga gttactgaac gtcggcagcc gccccgtccc cgtccgcata
     3481 cgtagcgtgc gggttgtcac gtttttgata cgatgggtgc cgagtctgtg tcgccgttgt
     3541 gatgccgccc gcgcgatgat gtagccagtg aacggggagt caagacggga caaccgccac
     3601 aggcacatcg cccaccgatc ccccggtgca ggaagagcgg
//
LOCUS       DASYWL010000244         2079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_86698, whole genome shotgun sequence.
ACCESSION   DASYWL010000244 DASYWL010000000
VERSION     DASYWL010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86698"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /gene="der"
                     /locus_tag="VGR22_08975"
     CDS             <1..1086
                     /gene="der"
                     /locus_tag="VGR22_08975"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEV2128737.1"
                     /translation="ISEADLIVLLVDARTGPTPQDQEIADMLRRSDKPVVLGANKAER
                     NDLRFEAMEFYNLGLGEPIPISAYHGNGTGDLLDRIVEELPNADELEEDEVEGPKIAI
                     VGRPNVGKSRLLNALLGEERAIVSDEPGTTRDSLDTVIEWEGQEVTLIDTAGIRRRGR
                     VEEGIERFSVMRSMRAIDRADVVLLVIDATESFTAQDLHIAGYVEEQKKGIIVVVNKW
                     DLIEKDSKSMDESRDRARRELDFMPYAPLAFISAKTGQRVGKVLEMALDVIEERNRRV
                     NTAALNRLLKDAVARHPPPSKPGKWIRFYYATQADVAPPTFVFFCNDPKAIHFSYRRY
                     IENELREAFSFSGTPLRISFRSRHEPN"
     gene            1089..1712
                     /gene="plsY"
                     /locus_tag="VGR22_08980"
     CDS             1089..1712
                     /gene="plsY"
                     /locus_tag="VGR22_08980"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEV2128738.1"
                     /translation="MIVLWSILGIAAAYLSGSVPWGVVLGRLIKGIDIRKIGSGNTGA
                     TNAMRALGWRIALSVAVLDVLKGFIPVIVARLLDAPTWTVALMGIAAIAGHCWSPWMM
                     FRGGKGMATGGGAAIGMLPWLLTMVPLMIIIVLLTRYVSLASIIASITGVTIAFIFAA
                     TTDFPWSWAASILAIISVIIFKHYSNIVRLLNGTERRFGAPEPVSGG"
BASE COUNT          358 a          689 c          661 g          371 t
ORIGIN      
        1 atctccgagg cggatctgat cgtcctgctt gtcgatgccc gcaccggccc cacaccgcag
       61 gaccaggaga tcgccgacat gctccggcgc tccgacaagc cggtggtgct cggcgccaac
      121 aaggcggagc ggaacgacct gcgcttcgag gccatggagt tctacaacct cggcctgggc
      181 gagccgatcc cgatctcggc gtaccacggc aacggtacag gggacctgct cgaccggatc
      241 gtcgaggagc ttcccaacgc cgacgagctc gaggaggacg aggtcgaggg accgaagatc
      301 gcgatcgtcg gccggccgaa cgtcggcaag agccggttgc tcaacgccct gctcggcgag
      361 gagcgcgcga tcgtctcgga cgagccgggc accacgcgcg actccctcga cacagtgatc
      421 gagtgggagg gtcaggaggt cacgctcatc gacaccgctg gtatacgccg gcgcggccgg
      481 gtggaggagg gcatcgagcg gttcagcgtg atgcgctcga tgcgcgccat cgaccgcgcc
      541 gacgtagtct tgctcgtcat cgacgcaacc gagagcttca ccgcccagga cctgcacatt
      601 gccggatacg tcgaggagca gaagaagggc atcattgtcg tcgtgaacaa gtgggacctc
      661 atcgagaagg actcgaagtc catggacgag tcccgcgacc gcgcgcgtcg tgagctcgac
      721 ttcatgccct acgcgccgct cgcgttcatc tccgccaaga ccggccagcg ggtcggcaag
      781 gtgctcgaga tggccctcga tgtcatcgag gagcggaaca ggcgggtcaa tactgccgcg
      841 ctcaaccggt tgctgaagga cgcggtcgcc aggcatccac ctccttcaaa gccgggcaag
      901 tggatccgtt tctactacgc cacccaggcg gacgtcgcgc cgccgacatt cgtcttcttc
      961 tgcaacgacc ccaaggcgat ccacttctcc tatcgccggt acatcgagaa cgagcttcgg
     1021 gaggcgttca gtttcagcgg aacgcccttg cgtatctcct tccggagccg gcacgagccc
     1081 aactgacgat gatcgtgctc tggtccatcc tcggcatcgc cgccgcctat ctctccggaa
     1141 gcgttccctg gggggtggtg ctcggcaggc tgatcaaggg catcgacatc cggaagatcg
     1201 gctctggcaa cactggggcg accaacgcca tgcgtgcact cgggtggcgc atcgcgctct
     1261 cggtggcggt gctcgacgtg ctcaagggct tcatcccggt catcgtggcg cggctgctcg
     1321 acgcgccgac atggaccgtg gcgctgatgg ggatcgccgc gattgccggg cactgctggt
     1381 cgccgtggat gatgttccgc ggcggcaagg gcatggcgac cggtggcgga gcggcgatcg
     1441 ggatgctgcc gtggttgctc accatggtgc cgctgatgat catcatcgtg ctgctgacgc
     1501 gctacgtgtc gctcgcatcg atcatcgcgt cgatcaccgg cgtcacgatc gcgttcatct
     1561 tcgcggcgac caccgacttc ccatggtcct gggcggcttc gatcctggcg atcatcagcg
     1621 tcatcatctt caagcactac agcaacatcg tcaggttgct gaacggcacc gagcgccgct
     1681 tcggtgcccc ggagcccgtc tccggcgggt agtgcaggcc gcggtcgtcg tcaactctct
     1741 gtgcacgcga cgatctcgtg ccggcccgtt tgtgagttcg cagagtgccg acttccccct
     1801 gtccatgaaa cccggccatc cgggcggcct tctcggcggt tatggattgc tgtcagattg
     1861 ctccgggaaa tctggtcgag cgaaaacccc accgatcctg gcagatcgga ggagactgcc
     1921 acttgcgaag acgatctcat tggcagcctt ccataagggc tccggtctct cgtcgactct
     1981 cagctccggt acccgtccag gtggcgctgc atccactccc acgccgagcc gatcatctca
     2041 tccagcgtgg agcgcgacgg ctgccagcca agcgtctcc
//
LOCUS       DASYWL010000245         3906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_443707, whole genome shotgun sequence.
ACCESSION   DASYWL010000245 DASYWL010000000
VERSION     DASYWL010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443707"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VGR22_08985"
     CDS             complement(<1..651)
                     /locus_tag="VGR22_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEV2128739.1"
                     /translation="MSVSQHPIGVPTRGDWSLPVRCMSCGAVHDGTRPITACPTCGGL
                     LDAVIDTSRKLTPDVFTAEPGSIRATSGVWRYAPLLPEIPEEMLVSRWEGNTPLYVEQ
                     RIADYAGLDHGQVRIKHEGHNPTGSFKDRGMTVALSHAKAIGSAMVACASTGNTSASL
                     ASYASSTGLPSLVLVPESKISAGKLAQTIAFGATVVQVEGDFDAALRLLRELTADGS"
     gene            complement(781..1008)
                     /locus_tag="VGR22_08990"
     CDS             complement(781..1008)
                     /locus_tag="VGR22_08990"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV2128740.1"
                     /translation="MPTLNIGLTRSRTNKWIFGVCGGIAEQYGMNPLAVRIGTVLLAI
                     IIPGVSVLPTILLYILLGLMLPEKGEPIDIS"
     gene            complement(1219..1902)
                     /locus_tag="VGR22_08995"
     CDS             complement(1219..1902)
                     /locus_tag="VGR22_08995"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class F sortase"
                     /protein_id="HEV2128741.1"
                     /translation="MPGILFMDQDTRISRRRIVGSALALASGLTLGRAALAQDDQEIV
                     PIQSTELGPMPSTGTIRDGFVSDSPDERQTTPGVRPAALVVDTAQIDAEIEVQQIVDG
                     VMQNPTGPWIVAWYEQTAELGELGNVVMAGHVDYWNVGPAVFFNLRDMVEGEEIEVIG
                     ENGTPFHYLVDWNETFDLEELNSGTITELVGPTDDPVLTLITCGGEFDYATGEYLSRT
                     VIRASLVGV"
     gene            2055..2993
                     /locus_tag="VGR22_09000"
     CDS             2055..2993
                     /locus_tag="VGR22_09000"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEV2128742.1"
                     /translation="MIHSLPQHAESTLKWLAEHGVIAKDAAREWIPAGTINTVIRTHD
                     DAGETVYVRIGPPAKVIGAPTWMRSDALANEVLALDRIRQAMSCLPMTVAAGFRSTAG
                     HWLVQKAVPGEPLDVRLPRLTVEERVAIWRELGELVRRLHEVNGPWFGTPAGDQRFER
                     WPTMVRSDAAGLLDDARLLGLNPSPYERLLVGIDRHEAALRAVDQPSAVHSDLNSRHV
                     FMGRRKGRWGITGIIDWEYARYVDPLSESLLVELLSRPEDDPDRAAFLGGYGFDLDLF
                     AEPTFRTRQKIYRGIIAGWVATDAARLNAARSANDV"
     gene            complement(3203..3871)
                     /locus_tag="VGR22_09005"
     CDS             complement(3203..3871)
                     /locus_tag="VGR22_09005"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEV2128743.1"
                     /translation="MTESRPAAQNPDELFDLVDATGTPLGQTKRRADVHRDGDWHRAV
                     HVWVFGTDDAGAFLLLQRRGMEKDTWPGKLDATAAGHLATGETPNDAFREIEEELGVA
                     PDLTILRHVGTRISVSERPPHWLDRELQEVYLLRDDQPLGIYRPSPAEVDALVRVSLD
                     AWTELLFGDRDDMSAEALEAGSGATATITTDREDPIPSIDGYFRRIAIACQRALAGER
                     YVVV"
BASE COUNT          662 a         1173 c         1347 g          724 t
ORIGIN      
        1 ggagccgtcg gccgtcaact cgcgcaggag gcgcagcgca gcgtcgaagt cgccctcaac
       61 ctgcacgacc gttgcgccga aggcgatggt ctgcgcgagc ttccccgcgc tgatcttgct
      121 ttccggcacc agcaccagcg agggaaggcc agtcgaggat gcgtaggatg cgagggatgc
      181 ggaggtgttg ccggtcgagg cacaggcgac cattgccgag ccgatcgcct tcgcgtggct
      241 cagcgcgacg gtcatgccgc gatccttgaa cgatccggtg gggttgtggc cctcgtgctt
      301 gattcgcacc tggccatgat cgagcccggc gtagtcggcg atgcgctgct ccacgtagag
      361 gggcgtgttg ccttcccagc ggctgacgag catctcctcg gggatttcgg ggagcagggg
      421 agcatagcgc cagacgcccg aggtggctcg gatcgatcct ggctcggcgg tgaagacgtc
      481 cggcgtgagc ttccggctgg tatcgatcac cgcgtcgagc agtccaccac aggtgggaca
      541 ggcagtgatc ggccgggtgc cgtcgtgaac cgcgccacag ctcatgcagc ggacgggcag
      601 ggaccagtcg ccccgggttg gcaccccgat ggggtgctgg gaaacggaca tgaacgcaag
      661 ttctcccgat ctcgatggcg gtgccgcgcg catgaagacg tgcatgcgcg aaggcgcgcc
      721 gtgccacgaa gtgcaacagc gcgcctcatc gctccgagga taccacggac ctcgcggcga
      781 tcaggagatg tcgatcggct cgcccttctc gggcagcatc aggccgagca ggatgtagag
      841 caggatggtc gggagtaccg aaacacccgg gataatgatc gccagcagca ccgtaccgat
      901 ccgtacggcc aatgggttca tgccgtactg ctcggcgatg ccgccgcaga cgccgaagat
      961 ccacttgttg gttctggacc gggtgagacc gatgttaagg gttggcatgc tcaaatcctt
     1021 tgcaatcagt gtaccgcctt ggcccattgc cttgggggcc ctcgtcaccg ttgccagtat
     1081 ccgtcagcgg cgggcagacg gcagtgggga tagccgccat ttaaccgggc agggcatccg
     1141 gaaacggggg gacggcacga aaaatgcccg gcatggatga ccgggcgctc atgatcaatc
     1201 acgatcaata aggtggagtc agaccccgac gagactcgct cggatgacgg tgcgcgagag
     1261 gtactcgccg gtcgcgtagt cgaactcgcc gccacaggtg atgagcgtca gcaccggatc
     1321 atcggtcggt cccacgagtt cggtgatcgt tccactgttg agctcctcga ggtcgaaggt
     1381 ttcgttccag tcgacgaggt agtggaacgg tgtcccgttc tcgccgatca cctcgatctc
     1441 ctcgccctcg accatgtccc tgaggttgaa gaagacggcc gggcccacgt tccagtagtc
     1501 gacgtgtccg gccatcacga cgttgccgag ctcgcccagc tcagcggtct gctcgtacca
     1561 cgcgacgatc cacgggccgg tcgggttctg catcacgccg tcgacgatct gctgcacctc
     1621 gatctcggcg tcgatctgtg ccgtatcgac caccagcgcc gccggtcgga cgccgggtgt
     1681 ggtttgccgt tcgtcgggtg agtcgctgac gaagccgtcg cgaatcgtgc cggtgctggg
     1741 catcgggccg agctcggtcg actggatcgg cacaatctcc tggtcgtcct gcgccagtgc
     1801 tgcgcgaccg agggtgaggc cgctggcgag cgcgagggca gagccaacga tccgccgccg
     1861 gctgatgcgt gtatcctgat ccatgaagag aattcccggc atcgaggtgc ctcctgcttc
     1921 gaaactgatg gccacgattc gcgcacgagc gaagcgatgc gatggcggag cgcgggttgg
     1981 gcctgtacca tgccattcta atctctcata cgcacgaccg tgccgattgg tgtcacgcac
     2041 gagggcgagt ggtgatgatc cacagcctgc cacagcatgc tgaatccaca ctgaagtggc
     2101 ttgccgaaca cggcgtcatt gccaaggatg ccgcgcggga atggatcccg gcaggcacca
     2161 tcaatacggt catccgtacg catgacgatg ccggagagac agtttacgtc cggatcggtc
     2221 cgccggcgaa ggtcatcggt gcgccgacgt ggatgcgcag tgacgccttg gcgaacgaag
     2281 ttctcgcgct ggatcggatt cggcaggcga tgtcctgtct gccgatgacc gtcgcagccg
     2341 ggtttcggtc aactgctggg cactggctgg tccagaaggc agtgcccggt gagccgctcg
     2401 acgtgcggtt gccgcgtctc accgtcgagg agcgagtggc aatctggcgt gagttgggcg
     2461 agttggtgcg tcgactgcac gaggtgaacg gcccctggtt cgggactccg gccggtgatc
     2521 agcggttcga gagatggccg acaatggtgc gatctgacgc tgccggtctg ctcgatgacg
     2581 cgcggttgct cgggctgaac ccctcgccgt acgagcggct gctcgttggg atcgatcgac
     2641 atgaggccgc gctgcgagcg gtcgaccagc catcggccgt tcacagtgac ctcaactcac
     2701 gacacgtgtt tatgggtaga cgaaaggggc ggtgggggat taccgggatc attgactggg
     2761 agtacgcgcg ctatgtcgat ccactgtccg aaagcctgct ggtcgagctg ctgagtcgac
     2821 cggaggacga tcccgaccgg gcggcgtttc tcggggggta tggattcgac ctcgatctgt
     2881 ttgccgagcc gacgtttcgg acgaggcaga agatctatcg cggcatcatt gccggctggg
     2941 tcgctactga tgctgctcgg ctgaatgctg cgcgaagtgc aaacgacgtt tagctccatt
     3001 agagcgagct gcatgacgat tgatgcaccg ctggttgggg ccgtagggtc aggtcttgtg
     3061 tctgctcgcg ctcggaccga cgcaagaatc tgtgctgagc gcagcgaagg atcgggccgt
     3121 acggaccggg cctgtgtcga gcacccttcc tgcaatcggc tgtgctctat ggccctgcgt
     3181 ttccatgcac gaggttgggt gattagacaa ccacgtaccg ctcaccggcg agggcgcgct
     3241 ggcatgcgat cgcgatgcgg cggaagtagc catcgattga tggtattgga tcctcgcgat
     3301 cggtggtgat cgtggcggtt gctcccgatc cggcttccag cgcttccgca ctcatgtcat
     3361 cgcggtcgcc gaacaggagc tctgtccatg cgtcgagtga tacgcgaacc agtgcgtcga
     3421 cctcggcagg gctcggccga tagattccca gcggctggtc gtcgcgcagg aggtagacct
     3481 cctgcagctc gcggtcaagc cagtggggcg gtcgctcgct gacgctgatg cgggtgccga
     3541 catgccggag gatggtcagg tccggcgcga cgccgagctc ctcctcgatc tcacggaagg
     3601 catcattcgg cgtctcgccg gtggcgaggt gacctgcagc cgtggcgtcg agctttccgg
     3661 gccaggtgtc cttctccatt ccgcgccgct ggagcaggag gaatgcgccg gcatcgtcag
     3721 tcccgaagac ccagacgtgg accgcccggt gccagtcgcc gtcacggtgg acatcggcgc
     3781 gccgcttggt ctggccgagc ggagtgccgg tggcgtcgac gagatcgaac agctcgtcgg
     3841 ggttctgggc ggcggggcga gactcggtca tcggtgctcc agtgcgagtg ctgtgcaccg
     3901 cccggc
//
LOCUS       DASYWL010000246        12302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_349967, whole genome shotgun sequence.
ACCESSION   DASYWL010000246 DASYWL010000000
VERSION     DASYWL010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 12302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12302
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349967"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VGR22_09010"
     CDS             <1..444
                     /locus_tag="VGR22_09010"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2128744.1"
                     /translation="HSHEESKVILKELRNLVRGIHPAVLSDRGLDDAISAIAGRSSIP
                     VEVDVDLANRLPGEVEGTAYFFVVEALANIAKHSHATEAHVNISRNGEWLRIEISDNG
                     LGGADPSRGTGLAGLRDRIQALDGRMRIESPNGGGTRLMVEIPCA"
     gene            435..1097
                     /locus_tag="VGR22_09015"
     CDS             435..1097
                     /locus_tag="VGR22_09015"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2128745.1"
                     /translation="MRVVIAEDAVLLREGVVRLLTEQGMEIIAAVADGEALIAATEQH
                     RPDVVIADVRMPPTFREEGLRAALEIRKRLPGTSILVFSQYVEERYAAELMATGTNGI
                     GYLLKDRVADVRDFIAAIRQVAAGGTVLDPEVVAQLVTRRRRDEHIASLTQREREVLE
                     LMAQGRTNAAIADALFVSEGTVEKHIRGIFGKLGLDDSIHDHRRVLAVLAYLDPQVNP
                     TL"
     gene            complement(1324..2031)
                     /locus_tag="VGR22_09020"
     CDS             complement(1324..2031)
                     /locus_tag="VGR22_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128746.1"
                     /translation="MRQALDEIGYQPVEVGGHEVLSIRGDFEQDLQAPTAYKFAGMNF
                     GTTLDGGTLVFASATAPLAAVLDVVSGTAPSMLDVADVSMLLQQAPDNLASALLVQGV
                     TLSGGEPAALLDIGSGGTPDVGTIATEMAVRSEMPPVAMALLGATVGGPAGRAEGVSP
                     DTPEARAIAVLLMLSPEAAETTAGIVEERLATGVSATTEEPYETMFPERAVQMVPGAP
                     VVRWNSRSVREFHQISS"
     gene            complement(2055..2495)
                     /locus_tag="VGR22_09025"
     CDS             complement(2055..2495)
                     /locus_tag="VGR22_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128747.1"
                     /translation="MQLERTASAIVRKSVYRLLMLVAVVLHGAVPAASQAQDPTPAAI
                     PTTTTVQDMFALVPAQLPGRDDVANATIAYADIEAHLDAVGATPPDSMDSPGFEGWAA
                     ATRFMTLPSPAQMLLQSFREDYGFDLLQVDQTLWVALPPFELAL"
     gene            complement(2598..3842)
                     /locus_tag="VGR22_09030"
     CDS             complement(2598..3842)
                     /locus_tag="VGR22_09030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HEV2128748.1"
                     /translation="MQPIHDLAATLMRRSDELAAHTEEPGKITRRYGTPALAEVMGIV
                     EGWMRDAGMQTRRDAIGNLFGLLAGPSDDCPRLMLGGHLDSVRDAGRYDGVLGVLSAI
                     ATVEVLRDREEPLPFAIEVVAFADEEGVRFHSLYLGSWSAIGALPPGLLDATDADGIT
                     VRQAIRDWGFDPEGALEGSLAECEYIGFVEAHIEQGPRLEVAGIPVGVVSGIVGTRRA
                     HLDVYGTAGHAGTVPMADRHDALVATSEIILAIDEGGRSHENAVATVGEIAVRPGASN
                     VITGGARISLDLRHPDGAVVDELYDEIRTRSAAIAARWKVDIAWRTGQKVEAMSCDDA
                     LSGALAAAVEAAGHEPVRLFSGAGHDAVAMSRVMPTTMLFTRCKGGISHNPAESIASE
                     DAAATIDVLVRFVDAMAAMHGR"
     gene            complement(3994..5637)
                     /gene="aceB"
                     /locus_tag="VGR22_09035"
     CDS             complement(3994..5637)
                     /gene="aceB"
                     /locus_tag="VGR22_09035"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEV2128749.1"
                     /translation="MAAFSPADQKGPFTMAQGIATASVSDDLEWVLSDEALAFVADLH
                     RTFNQRRKELLGAREDRLARILAGEEELRFLDETADVRSGEWSVAPAPADLNDRRAEI
                     TGPCDRKMVINALNSGAKVFMADLEDASSPTWHNVVDGQRNLYDAVRRTITFQNPDGR
                     TYQLNDKVATLLVRPRGWHLHEAHITVDGEPVSGSLVDFGLYIFHNGKELIKRGSGPY
                     FYIPKLESHLEARLWNDVFTRAQEQLGIPYGTIRATCLIETLPAAFEMEEILYELRDH
                     AAGLNAGRWDYIFSAIKTFHNRPDKILPERSQVTMTVPFMRAYTELLVRSCHKRGAHA
                     IGGMAAFIPTRRDPEANDRAMAAVHADKKREAGDGFDGTWVAHPGLVPVAIEEMTAVF
                     GDAPNQKDRLREDVSVDASELTDFTVPGGTITESGVRTNVSVAIQYIASWLSGVGAAA
                     INNLMEDAATAEISRAQLWQWIHHDQTTAEGTPITKAWYRQVLDQELGKLSGDYPFAE
                     AVEILDELVLSDAFITFLTLPAYQRLIASEERIPSEGSA"
     gene            6597..7409
                     /locus_tag="VGR22_09040"
     CDS             6597..7409
                     /locus_tag="VGR22_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128750.1"
                     /translation="MDQERLLASIQRGIRLDLFNAPADAVRELEGSVWGPLRHQPALI
                     EPLLSQPVVGSWLARSAVQLSHALIETGRWGSALTWLRLIDGWLSRPGSELDQEQLAG
                     QHWTTGPGDSWQHGGAIPAGAERVSLRAADLWIEAQQYLALVQRDGATEVPVERLAHV
                     QNVLRGRPLGRRFSPECIARYRGVIGHDLASLKLRRGYPPSMVLQDLDAAEAMLRAAG
                     NTSMLAPTMLARASALGRLLDTEPIDRRLLNSVEHSVSDAMRQGEADPVLSI"
     gene            7422..7616
                     /locus_tag="VGR22_09045"
     CDS             7422..7616
                     /locus_tag="VGR22_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128751.1"
                     /translation="MESIDLLVGLRGPEPEDVDRLAAAAAHAIERRFVIQARKILQCK
                     HLLALVPDLEPDLRAVAQAS"
     gene            complement(7717..9702)
                     /locus_tag="VGR22_09050"
     CDS             complement(7717..9702)
                     /locus_tag="VGR22_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEV2128752.1"
                     /translation="MTTLRPTTLLWRRTRTALLATSIAIGSVMAPTGASTLADSGSAA
                     SADGTSGASTFVWEGSSLSDATAIVAAGGQNLNLRAEPDGDADVVAKIPHGAVVALRI
                     DETDTVLDAASGTRWWPVEYNGVDGWASGHNLVDGTGLEPGDVPFEFPGGSLADAEAR
                     VRAEAGLRLRADPDVDAKVLATIPNGTVVELRIDETDTVLGPDGVTRWWPVTFNGHEG
                     WVSGYYLVDPDAPATTTAAAVTKDVPDTAVAAPTEQFEWDGGALEGATAIAAGFGENV
                     NVRAEATEASEIVAKVPDGTIINLRIDRVDTVIGPDGETRWWPVVVDGVEGWVSGFYL
                     TDGTQATVSSDIFPVGSIAMVQTPTGEGLRLRASAGMDAEQIASLPDRSQVTILSGSV
                     SQENSTNGWFQVRAESGLTGYVDGDLLILLATPVAEPATEGRAPATVDEAATESESDG
                     TDASVPSPTATETPVTPVTRTTDEEAGDERSPQRQETPTAAAADDDERADQPTPTPAE
                     EIRETPPPPAQAPTQSASSQFIVPVAGATRTQDFGCSSLGFYPYNPDWGCGVHDGIDF
                     AAPAYTPIVAAAAGTVVTAGWCDCGLGYYVEIDHGNGVHTVYGHMAAQPPVSVGQRVS
                     QGETIGSVGSSGLSTGPHVHFMVQVNGVTQDPNRYLS"
     gene            10430..12262
                     /gene="tig"
                     /locus_tag="VGR22_09055"
     CDS             10430..12262
                     /gene="tig"
                     /locus_tag="VGR22_09055"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEV2128753.1"
                     /translation="MKLSVERQAGSLVLLDITAEDEEFSQAMDRAFRRAAKDIQVPGF
                     RKGKAPRTIIERYYGREVFVQDAAEEVMDSLYRKALEQEEITPVGEPDVSIESLEPVN
                     FKVTVPVYPTIETGDYTSVRVEPEDASVTDEDVNEVLERLQKSQATWSDPAEPRTPRE
                     GDQVNVDYEVKEGDEDFQEPVKDAQFVLGETNLLQPLRERIEEMHVGDTETFDLVFDE
                     NDETADPSIRGKTLTYTVTLNSVKERELPEIDDELAKSAADAGSLEDLKAQIRDDVHQ
                     GKTTEARQNVVNNIINQIAENAEVDPPAVMVDEEVQHQLNHLKQNLAQSGTPYEAYLR
                     AQNTTEEQVKEELRPEAERRLRNSLILQEIGRREQVEVTDADIDAELDRILGPVGDDE
                     QSQRMREMYQSDYFRNLIRNEMFDRKLTDRIIEIATEGKVAVTNAWEPPTEEEAAQVA
                     ARAADAESEAGTATEEAPTSEEGSAETEQAAASGVASEPAAGAAPTTAEATNSVITNE
                     TAEEGNQIAEDVVAGEGDNVQKSAEASPKLEGERGEGWVKGDGTNNPPEGYPIKGNAN
                     SMIYHPPESRSYANTVAEYYFATAEVAESFGFRPPRGINRTSQQ"
BASE COUNT         2152 a         4159 c         3986 g         2005 t
ORIGIN      
        1 cactcccacg aggagtccaa ggtcattctc aaggagttgc gtaatctcgt ccgcggcatc
       61 caccccgccg ttctgagcga tcgcggtctc gacgacgcca tctcggcgat cgccggacgc
      121 tcctcaatcc ctgtcgaggt cgatgtcgat ctcgccaacc gcctgcccgg cgaggttgag
      181 ggcaccgcct acttcttcgt cgtcgaggcg ctggcgaaca tcgccaaaca cagccatgcc
      241 accgaggcac acgtgaacat cagccgcaac ggcgagtggc tccgcatcga gatcagcgat
      301 aatggcctcg gtggagccga tccgtcccgg gggacggggt tggccgggtt gcgcgaccgc
      361 attcaggcgc tggatgggcg aatgcgcatc gagagcccga acggaggtgg cacccggctg
      421 atggtggaga ttccatgcgc gtagtgattg ccgaggatgc tgtgctgctg cgcgaggggg
      481 tcgtgcggct gctgacggag caggggatgg agatcatcgc cgcggtcgcc gatggcgagg
      541 cgctgatcgc ggcgaccgag cagcaccggc cggacgtcgt catcgccgat gtccgcatgc
      601 cgcccacctt ccgtgaggaa ggcctccgcg ccgccctgga gatccgcaag cgcctgcccg
      661 ggacgtccat cctcgtcttc tcccagtacg tcgaggagcg ctatgccgcc gagctgatgg
      721 cgaccggcac aaacggtatt ggctacctcc tcaaggatcg cgtcgcggat gtccgcgact
      781 tcatcgctgc gatccggcag gtcgccgccg gcggcaccgt gctggacccc gaggtggtcg
      841 cgcagctggt cacccgtcgt cgtcgcgacg agcacatcgc aagcctgacc cagcgggagc
      901 gcgaggtgct ggagctgatg gcgcagggcc gaaccaacgc cgccatcgcc gacgcactct
      961 tcgtctccga ggggacggtc gagaagcata ttcgcggcat ctttggcaag ctcggcctcg
     1021 acgactccat ccacgaccac cgccgggtcc tcgccgtcct cgcctacctc gacccccagg
     1081 tcaatcccac cttgtaactc gttcactacc ttccagatat caatttccca caactcccag
     1141 cagatgacgc cagtgccgca gggttcaacc tcccgtacgg agttgtcgac ccatctcaag
     1201 gagcggatcg acagtggccg ggtcgaccgc tgctgcgcag cgtggtccct acaggtacgc
     1261 tacaaagttt gtgccacccc tctcaccaag caaggaagcc gagatcccgg ctgaatagca
     1321 tgctcacgag gatatttggt ggaactccct caccgagcgt gagttccacc gcaccaccgg
     1381 cgcccccggc accatctgga ccgcccgctc cgggaacatg gtctcgtacg gctcctcggt
     1441 ggtggccgag accccggtcg caagccgctc ctcgacgatc cccgcggtgg tctcggcagc
     1501 ctcgggactg agcatcagca gcaccgcaat cgcacgcgcc tcgggcgtgt ctggcgacac
     1561 cccctcagct cgtcccgccg gtccacccac ggtcgccccg agcagtgcca tcgccaccgg
     1621 tggcatctcg ctccttacgg ccatctccgt cgcgatcgtg ccgacatccg gtgtcccgcc
     1681 cgagccaatg tccagcagcg ctgctggctc gccgccggag agcgtcacgc cctgaacgag
     1741 cagggcggac gcgagattat ccggcgcctg ctggagcaac atcgagacat cggcgacatc
     1801 cagcatcgac ggcgcagttc ccgagacgac gtcgagcacg gccgccaatg gcgccgtggc
     1861 ggaggcgaat accagcgtcc cgccgtcgag agtcgtcccg aagttcatcc ccgcgaactt
     1921 gtaggctgtc ggcgcctgga gatcctgttc gaaatcgccg cggatcgaaa gcacctcgtg
     1981 tccgcccacc tcgaccggct ggtacccgat ctcatcgagc gcctggcgca cctcatcgta
     2041 atcgaagccc cccgttagag tgcaagctcg aatggcggca aagccaccca cagggtctga
     2101 tcgacctgca gcaagtcgaa cccgtagtcc tcgcggaagg actggaggag catttgggct
     2161 ggcgagggca gcgtcatgaa cctcgtagcg gcagcccatc cctcgaagcc tggcgagtcc
     2221 atgctgtccg gcggggtggc cccgacggca tcgagatggg cctcgatgtc agcgtaggcg
     2281 atcgtggcgt tcgcgacgtc gtcccgcccg gggagctggg ccgggaccag cgcgaacatg
     2341 tcttgcaccg tggtggtcgt gggtatcgct gcaggcgtgg ggtcctgtgc ctgcgacgcc
     2401 gccggtacag cgccgtgcag gacaacggca acgagcatca gcaaacggta caccgacttc
     2461 cggacgatcg cgctcgcggt tcgctccagc tgcatcgatt gcctccccca cttcactaca
     2521 gcatgatcat gtcggacccg acgcgcactc tactccacgc ggccgttttt caagcccgca
     2581 ttttcgtgac tacaccgtta tcggccatgc atcgcggcca tggcatcgac gaaccggacg
     2641 agcacgtcta tcgtggcggc cgcatcctcg ctggcgatgc tctccgccgg attgtggctt
     2701 atgccgccct tgcagcgcgt gaagagcatc gtcgtcggca tcacccgcga catcgccacc
     2761 gcgtcgtgcc ccgccccact gaacagccgc accggttcgt gtcccgccgc ctcaaccgcg
     2821 gccgccagcg cgccgctgag cgcgtcgtcg cacgacatcg cctcgacctt ctgtccggtc
     2881 cgccaagcga tgtcgacctt ccaccgcgcc gcgatcgccg ccgaccgggt gcgaatctcg
     2941 tcgtacagct cgtcgaccac cgcgccgtcg gggtgccgca ggtcgaggct gatccgcgcc
     3001 ccgccggtaa tcacgttgct cgcaccgggc ctgacggcga tctctccgac cgtcgcgaca
     3061 gcattctcgt ggctgcgacc gccctcgtcg atcgccagga tgatctcgct cgtcgccacg
     3121 agggcgtcgt gacgatccgc catcggcacg gtgccggcgt gcccggcggt gccgtagaca
     3181 tcgagatgcg cccggcgtgt gccgacgatg ccggagacga cgccgaccgg gattcctgct
     3241 acctcaaggc gcggcccctg ctcgatgtgc gcctccacga agccgatgta ctcgcactcc
     3301 gcgagcgagc cctccagcgc cccctccgga tcgaagcccc agtcccggat cgcctgccga
     3361 acagtgatgc cgtcggcatc tgtggcgtcg agcaaccccg gcgggagtgc tccgatggcc
     3421 gaccacgagc cgagataaag cgagtgaaac cggaccccct cctcgtccgc gaaggcgact
     3481 acctcgatgg cgaacggcag cggctcctcc cgatcacgaa gcacctcgac ggtggcgatc
     3541 gcgctcagca cgccgagcac gccgtcgtac ctgccggcgt cccgaacgga gtcgagatgc
     3601 ccgccgagca tcaggcgcgg gcagtcatcg ctcgggcccg ccaggagacc gaagaggttg
     3661 ccgatggcat cgcggcgagt ctgcataccg gcgtcccgca tccagccctc gacgatcccc
     3721 atcacctcgg caagggcggg cgtgccataa cgccgggtga tcttccccgg ctcctccgtg
     3781 tgcgccgcga gctcatcgga gcgccgcatc agcgttgcgg cgaggtcgtg gatgggttgc
     3841 atgcaattcg cctcttgagc ccaactacca gtagacacag ccgccacccc gatcttgtcg
     3901 agggatccct gcgcgaagcg ctcggctgcg cactttgccg catcaaggat cactgcaacg
     3961 cgcgagacct ggggccgttt cacggctcct gaatcaggcg gagccttccg aagggatcct
     4021 ctcctctgag gctatgaggc gctggtaggc cggcagcgtc aagaaagtga tgaacgcgtc
     4081 actcagcacc aattcgtcga ggatctccac cgcctcggcg aacgggtagt cgccggagag
     4141 cttgccgagc tcctggtcga gcacctgccg ataccatgcc ttcgtgatcg gcgttccctc
     4201 cgccgttgtc tggtcgtggt ggatccactg ccagagctgc gcccgcgaga tctcggcggt
     4261 ggccgcatcc tccatcaggt tgttgatcgc cgccgcgccg acaccgctga gccacgaggc
     4321 gatgtactgg atcgcgacgc tgacgttggt ccgcacgccg ctctcggtga tcgtgccgcc
     4381 cggaacggtg aaatcggtca gctcgctcgc gtcgaccgag acatcctcac gcaggcgatc
     4441 tttctggttg ggcgcatcgc cgaaaaccgc ggtcatctcc tcaatcgcga ccggcacgag
     4501 acccgggtgc gcgacccagg tgccgtcgaa accgtcacct gcctcgcgct tcttgtcggc
     4561 atggacggcc gccatcgccc gatcgttcgc ctccgggtcg cgtcgggtcg ggatgaacgc
     4621 cgccatgcca ccgatcgcgt gagcgccgcg cttgtggcaa ctgcgcacca ggagctcggt
     4681 gtacgcgcgc atgaacggga ccgtcatcgt gacctgcgag cgttccggga ggatcttgtc
     4741 cgggcggttg tggaaggtct tgatcgcact gaagatgtag tcccagcgac cggcattgag
     4801 cccggcggca tggtcacgga gttcgtagag gatctcctcc atctcgaacg cggcggggag
     4861 ggtctcgatc aggcaggtcg cgcggatggt gccgtacggg atgccgagct gctcctgcgc
     4921 tcgcgtgaag acgtcgttcc agagccgcgc ctcgagatgg ctctccagct tggggatgta
     4981 gaagtatggc ccggagcccc gcttgatcag ctccttgccg ttgtggaaga tgtagagccc
     5041 gaagtcgacc agactgccgc tgaccggctc gccgtcgacg gtgatgtggg cctcatggag
     5101 gtgccagcca cgtggccgaa cgagcaaggt cgcgaccttg tcgttgagct gataggtgcg
     5161 gccgtcggga ttctggaagg tgatggtccg gcggacggcg tcgtagaggt tgcgctggcc
     5221 gtcgacgacg ttgtgccacg tcggggacga ggcgtcctcg agatcggcca tgaagacctt
     5281 cgcgccgctg ttaagcgcgt tgatgaccat cttgcggtcg cacggcccgg tgatctcggc
     5341 gcggcggtcg ttgaggtcgg ctggcgccgg ggcgaccgac cactcgccgg agcggacatc
     5401 agcggtctca tcgagaaagc gcagctcctc ctcgccggcg aggatgcggg cgaggcgatc
     5461 ttcgcgcgcc ccgagcagct ctttccggcg ctggttgaag gtgcggtgga ggtccgcgac
     5521 gaaggcaagc gcctcgtcgg agagcaccca ctcgaggtca tcggaaacgg aagcggtcgc
     5581 gatgccctgc gccatggtga acggtccctt ctggtcggca ggcgaaaacg ctgccacacg
     5641 atgtcagtcc gtggccatct tctgacagcg ttcgaccgaa tgcaagacag ggtgcgactc
     5701 aggcgacgga acccgatgag ccggtgtctt ccaggacccg ggttcgccgc aatgatccct
     5761 agacgggcaa gtgacgaacg gtccgtgcat cgctggtgga cctgaatcag cgtgtgctgt
     5821 tggcaacagc gtgataccga catgtcgtgt gagcattgcc atcccgtgct cgcgccatgc
     5881 tgcagacgcc tcccaccgct cagcctgcat tgaagacgcc atgttgccga caccgagggc
     5941 ctcggacatg acgaggcggg acatgcagag ggcatgaccc gcttgtcggt cgtcaccaac
     6001 ggcatcagca gtgcagccgg gatccttggg gggcatcatc tgcgattccg gtaccggccc
     6061 gcttgcgagc gggccgctcg ccgacagccg tggcacgcct taatcatctg cctgcgcaga
     6121 gaacgcgtcc aatggtgggt tcgggaggtg tacaccttga cgtgacggca ctacagtttc
     6181 cccacccaac cgttggggaa ggagcacatc atggcaagca ccaccgctca gcgatctgcg
     6241 ttcattgccg catggcttcg gcacggaaat gtccgccgcg ccatgcggga gaccggggtg
     6301 ggtagcccgt ccaccgccta tcgatggatc catgcctttg agcgacatgg atcagtgaca
     6361 gcgccggagc aggctgcaag ccgacccagc aggatcgccc ccgacgtagg tgccgaggtg
     6421 gtagccatgc gagaggcgca tcccacctgg ggcaaacttc ggatcgcccg ccggctgtcc
     6481 gagctccagg gcaagccggt gatcaacccg acagacgttc gatcggtgct cgcgaggcgg
     6541 ggttgtgggc tccgcctcca gtcggcgacg cgcccggcgc cacggtggtg gatgaggtgg
     6601 atcaggagcg gctgctggcc agcatccagc ggggcatccg tttggatctg tttaatgctc
     6661 ccgcagatgc agtgcgcgaa cttgagggtt cggtttgggg tccgcttcgg catcagccag
     6721 cgctcatcga gccgcttctc agccagccgg ttgtcggctc ctggctggcc cgcagcgcag
     6781 tccagctctc gcacgcattg atcgagacgg gtcggtgggg atcagccctc acctggctcc
     6841 ggctgatcga cggctggctt tcccgaccgg gctcggagct ggatcaagaa cagctggccg
     6901 gccagcactg gacaactggt ccgggtgaca gctggcagca cggcggcgcc atcccagcgg
     6961 gcgcggagcg tgtgagtctg cgtgctgccg atctctggat tgaggcgcag cagtatctcg
     7021 cgctggtgca gcgcgatggt gccaccgagg tcccggtcga gaggcttgct cacgtgcaga
     7081 acgtcctccg cggacgacct cttggacgaa ggttcagccc cgaatgcatc gcccgatacc
     7141 ggggcgtcat cgggcatgac ctcgcctcgc tcaagctgcg tcgtgggtac ccaccgtcca
     7201 tggtcctgca ggatctggac gcggcggaag cgatgcttcg tgcagcgggt aacacaagca
     7261 tgcttgcccc gacaatgctc gccagggcaa gtgcgctcgg ccgcctgctg gacaccgagc
     7321 ccatcgaccg tcgcctgctc aactcggtgg aacattccgt cagtgatgca atgcggcaag
     7381 gggaggcaga tccggtgctt tccatctagg tgcgcgtggc gatggagagc atcgacctgc
     7441 tggtcggcct ccgcggccca gagccggagg acgtcgaccg gctcgcggca gcggccgctc
     7501 acgcaattga acggcgcttt gtgatccaag cgcgcaagat cctgcagtgc aaacacctgc
     7561 tggcacttgt ccccgatctc gaacctgatc ttcgggccgt tgcgcaagct tcctgaacgg
     7621 cccgctccgt tcccgtcccg atgatccgaa tgtttggtaa tcgcacgacc ccggaggaaa
     7681 attccgaggt cgtgcgactg aaacatgtct ttgctgctac gacaggtagc gattgggatc
     7741 ctgggtgacg ccattgacct gcaccatgaa gtgaacgtgc gggccggtgc tgaggccgga
     7801 gctgccgacg gagccgatcg tctcgccttg gctcacccgc tggccgacac tcacgggagg
     7861 ttgggcagcc atgtgcccat agacggtatg cacgccgttg ccgtggtcga tctcgacgta
     7921 gtagcccagc ccgcagtcgc accagccggc ggttaccaca gtgccggcag cggcggcgac
     7981 gatcggcgtg taggccggcg cggcgaagtc gataccgtcg tggacgccgc agccccaatc
     8041 cgggttgtac ggatagaaac cgagcgaact gcacccgaaa tcctgggttc gcgtcgcgcc
     8101 cgccacggga acgatgaact gactgctggc actctgggtc ggcgcctgcg ccggaggcgg
     8161 cggcgtctct cgaatctcct cggcaggagt tggcgtcggc tggtccgccc gctcgtcgtc
     8221 gtcagcggca gcggctgtcg gcgtctcctg gcgctgcggc gacctctcgt cacccgcctc
     8281 ttcatcggtg gtgcgagtga ccggggtgac gggtgtctcg gtcgctgtgg gcgagggcac
     8341 cgacgcatcg gtcccatcgc tttcgctttc ggttgccgct tcgtcaaccg tggcaggcgc
     8401 ccggccctcg gtggcaggct cggcgaccgg tgtggcgagc aggatcagca ggtcgccgtc
     8461 gacgtagccg gtgagcccgc tctcggcgcg gacctggaac cagccgttgg tgctgttctc
     8521 ctggctcacc gagccactga ggatggtcac ctgcgagcgg tccggcagcg aggcgatctg
     8581 ctcggcatcc atgccggcac tcgcccgcag tcgcagccct tcgccagtgg gcgtctgtac
     8641 catcgcgatg gacccgaccg ggaagatgtc ggagctgacg gtggcctggg tgccgtcggt
     8701 caggtagaag ccggaaaccc agccctccac gccgtcgacc accaccggcc accagcgcgt
     8761 ctcgccgtcc ggtccgatca cggtgtcgac ccggtcgatg cgcaggttga tgatggtgcc
     8821 atccggcacc ttggccacga tctcgctcgc ctcggtcgcc tcggcgcgga cattcacgtt
     8881 ctcgccgaat ccggcggcga tcgcggtggc cccctctagc gcccctccgt cccactcgaa
     8941 ctgttcggtc ggtgcagcca ccgccgtatc cgggacgtcc ttcgtcactg ccgccgcagt
     9001 ggtcgtcgcg ggtgcgtccg ggtcgacgag atagtaaccg gaaacccagc cttcatggcc
     9061 attgaaggtc accggccacc agcgagtcac cccatcggga ccgagtacgg tgtcggtctc
     9121 gtcgatgcgc agctcgacca cggtcccgtt cgggatggtc gccagcacct tcgcgtcaac
     9181 atcgggatcc gcacgcaggc gcagccccgc ctcggcgcgc acgcgtgcct ccgcgtcagc
     9241 cagcgatccc cccgggaact caaagggaac atcgcccggt tctaagccag taccgtcgac
     9301 caggttgtgg ccactcgccc agccatcgac gccgttgtac tccaccggcc accagcgcgt
     9361 gcccgaagca gcgtcgagca cagtgtcggt ctcgtcgatg cgcagcgcca cgaccgcacc
     9421 gtgcgggatc ttcgccacca cgtcggcatc accatccggt tcggcacgca ggttgaggtt
     9481 ctgcccgccg gcagcgacga tcgccgtcgc atcggaaagc gagctgccct cccacacgaa
     9541 tgtgctcgcc cccgacgtac catcggccga ggcggccgat ccgctgtcgg ccagcgtgct
     9601 ggcaccggtc ggagccatca cagagccgat cgcaatcgac gtggcgagca gggccgttcg
     9661 cgtgcgtcgc cagagcaagg tcgtggggcg taacgtggtc aacgtgcctt cccttccgag
     9721 gcgaccaaca agaccgacgg ccgcgtgtaa cgctgcaaaa tgcgcgaacc agttgttcat
     9781 ctgattgcct gtcgacagcg cccgcgcggc cggcggtggt ttccggcctc cgagggagct
     9841 agccacgtcc ccccgctatg tccatccccg gttaccggag ccggccgccg agcgctctct
     9901 ccagaccctg cctcgccgca gtggcggccc ggcgagtccg gctgtctgat caggatcgcg
     9961 cgcgctgtgc ggaatgcacg tacattccta tcagactgta catgcacaat accaccgtcg
    10021 acgtcccgtg acaatgtgaa cgtgcccgaa tgggcacccc ggagaatcgg aatgcggaga
    10081 tgtccctgac ctgatatgtg acagcgagac acaggattcg ccgcgcgttg ctcgccggtg
    10141 ctatactccg ttgcggacag ggtggccgtg caagcggtca tattggtgtg cccggtgaaa
    10201 gaactgggcc cgagccgacg ttcgacagtg cacgtgtact gctcgccagg cacaggacca
    10261 aacgtgtcac tcgggctgca atgcctgcac gacagcaacg cgattccggg tggtgcgacc
    10321 gacccgccct tccttatgtt gcaggaagaa cgctgtggtc gaaccgcgca tccacggcac
    10381 cgcaacggtg tcccaccccg gaccggcatg tagaagggac gcgacaggcg tgaaactttc
    10441 cgtagagcga caggctggca gcctcgtgct gctcgatatc accgcagagg acgaggaatt
    10501 ctcccaggcg atggaccgtg ccttccggcg cgccgccaag gacattcagg tccccggctt
    10561 ccgcaagggc aaggctccac gcaccatcat cgagcgctac tacgggcgcg aggtcttcgt
    10621 gcaggacgcc gccgaggagg tgatggattc cctgtaccgc aaggccctcg agcaggagga
    10681 gatcacgccg gtcggcgagc cggatgtctc catcgagtcg ctcgagccgg tgaacttcaa
    10741 ggtcactgtg ccggtctacc cgaccatcga gacgggtgat tacacatcgg ttcgggtcga
    10801 gccggaggac gcctccgtca ccgatgagga cgtcaacgag gtgctggagc gcctgcagaa
    10861 gtcgcaggca acctggagcg acccggccga gccgcgcacg ccgcgcgagg gcgaccaagt
    10921 caacgtcgac tacgaggtca aggagggcga cgaggacttc caggagcccg tcaaggacgc
    10981 ccagttcgtc ctcggcgaga ccaatctcct ccagccactg cgcgagcgca tcgaggagat
    11041 gcacgtcggc gacaccgaga cgttcgacct ggtcttcgac gagaacgacg agacggccga
    11101 cccctcgatc cgcggcaaga cgctgaccta caccgtcacg ctcaacagcg tcaaggagcg
    11161 cgagctgccg gagatcgacg acgagctcgc caagagcgcc gccgacgccg ggagcctcga
    11221 ggacctcaag gcgcagatcc gcgatgatgt ccaccagggc aagaccaccg aggcgcgaca
    11281 gaacgtcgtc aacaacatca tcaaccagat cgccgagaat gccgaggtgg atccaccggc
    11341 ggtgatggtc gacgaggagg ttcagcacca gctcaaccat ctgaagcaga acctcgcgca
    11401 gagcggcacg ccgtacgagg cctacctccg cgcccagaac accacggagg agcaggtcaa
    11461 ggaggagctc cggcccgaag cggagcggcg cctgcgcaac tcgctgatcc tgcaggagat
    11521 cgggcgccgg gagcaggtgg aggtcaccga cgcggatatc gatgcagagc tcgatcgcat
    11581 cctcgggccg gtcggtgacg acgagcagag ccagcgcatg cgcgagatgt accagagcga
    11641 ctacttccgg aacttgatca ggaacgagat gttcgaccgg aagctgaccg accgcatcat
    11701 cgagatcgcc accgagggca aggtcgcggt caccaacgcc tgggagccgc cgacagagga
    11761 ggaggccgcg caggttgccg cccgagctgc tgacgcagag tcggaggcgg gcacggccac
    11821 cgaggaagca cccacgagcg aggaaggttc cgccgaaacc gaacaggccg ccgcgagtgg
    11881 agttgccagc gagccagccg cgggcgccgc acccactact gccgaagcaa ccaactcggt
    11941 gatcaccaac gagacggccg aggaaggcaa ccagatcgcc gaggatgtcg ttgccggcga
    12001 gggcgacaac gttcagaagt cggccgaggc gtctccgaag ctcgagggcg agcgcggcga
    12061 gggctgggtc aagggcgacg gcaccaacaa cccacccgag ggctacccca tcaagggcaa
    12121 cgccaactcg atgatctacc acccgccgga atcgcgctcc tacgccaaca ccgtcgcgga
    12181 gtactacttc gcgacggcgg aggtcgccga gtcgttcggc ttccggccgc cacgcggcat
    12241 caatcgtacc agtcagcagt aaggagcacc ttcactgcga gagagatagg aagagtggcg
    12301 gg
//
LOCUS       DASYWL010000247         4270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_387374, whole genome shotgun sequence.
ACCESSION   DASYWL010000247 DASYWL010000000
VERSION     DASYWL010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387374"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="VGR22_09060"
     CDS             <1..677
                     /locus_tag="VGR22_09060"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEV2128754.1"
                     /translation="PVSIDAGFLLWFFGRRVESLPERLPVLATLLSTLGLGLPAGLNA
                     WLPFLVLSLADRYTTVVELDGPFEFISSTPGLIIILLLLPIELIVDKIPGADHISDLV
                     HTFVRPVAGAILAAAVADASGDVDAWLGAAVGGASAGATHAAKMSTRPVITASTGGVG
                     NPVASVVEDFITTVTSIIAVFLPLLLLVVLPLAAVGLFALWQRLKRGSERLRAAARRS
                     SPTMRT"
     gene            743..1795
                     /locus_tag="VGR22_09065"
     CDS             743..1795
                     /locus_tag="VGR22_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEV2128755.1"
                     /translation="MTPVQFKDYYAILGVDRDADQKEIKRAFRKKARENHPDVNRDDP
                     AAESRFKDINEAYEVLSNTEKRQMYDRYGAEWQRYRDAGFDASGPAPDAGPQYGSTGD
                     FEQWFTGDTGGFTRTTTSGGAGGGFSDFFNLLFGQDGGRGGFRQAVRPVRGQDLEVAA
                     TVTLDEAYHGTTRRLTVRAPKPCDLCDGTGVVRGAMCPRCDGSGEILEPRTLEVKIPA
                     GVRTGSRIRVRGQGGPGQNGGASGDVYLQVTVADHPRFERTGDHLRTTVNVPLYDAIL
                     GGEVQVPTMTGGVMLTIPPETQSGKVFRLRGKGMPKLGKPNQHGDLLVTVEVTIPTEL
                     SEEEKRRFTELRNLRQ"
     gene            1874..3154
                     /locus_tag="VGR22_09070"
     CDS             1874..3154
                     /locus_tag="VGR22_09070"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEV2128756.1"
                     /translation="MTATIIMGGQWGDEGKGKLTDALASAVSMVVRANGGSNAGHTVK
                     NDQGTFKLHLVPSGILNPNCLCVIGAGVVVEPDTLIQELDELEQRGIDTSRLRISDRA
                     HIVMPYHPVLDRLEEQRREDLSIGTTLRGNGPAYADKMSRHGIRIGDLLSGNVLRSRV
                     RWEVDQKNEIISRLYRAEPLDASEILHKAESWAERLARYIRPTQVDVQDALSAGEHVL
                     IECAQGAMLDIDYGTYPFVTSSSPTAAGACQGAGVAPRQVERVVAVYKAYSTRVGAGP
                     MPSELKDETGERIRQRGNEFGTTTGRPRRTGWFDEVVARQSARLNGVDEVALTLLDVL
                     DDFETIKTCTAYRRGSDVISHLPAVLNDAENAEPVWQEHVGWQTDTTGARAWDDLPPA
                     AKSYVQHIGSVLNARVRYIGIGPAREQLIDRGPV"
     gene            complement(3163..4173)
                     /locus_tag="VGR22_09075"
     CDS             complement(3163..4173)
                     /locus_tag="VGR22_09075"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV2128757.1"
                     /translation="MKVTVVGSGAWGTTLSGVAVDAGSEVTLVARNPAVLTSLRDERR
                     HPVSVAGYRLPDAVAVVSSVAEGLAEAPDMVLVVVPSSAVDAAAGEIEAAGYRGLVVT
                     ATKGIDPGSLKTPAARVASVRGTGSPVAALSGPNLAGEVATGLPAAAVIASESEETAG
                     FARTALMSSRFRIYTSRDVIGVEIAGAFKNVIAIGAGISDGLDAGQNAKAAYVTRGIA
                     EMARLGLACGADVMTFAGLAGIGDLMATCNSERSRNHTVGRGIAKGRSLESVLANMRE
                     VAEGVQTTRAVLDLAQVKGVELPIAAQIARILFEGVSPQTAIADLMARDATSEMALLE
                     GF"
BASE COUNT          760 a         1412 c         1359 g          739 t
ORIGIN      
        1 accccgtgtc gatcgacgcg gggtttttgc tatggttttt cggtagacgc gtcgagtccc
       61 tcccggagcg attgcctgtg ctcgccaccc tcctgtcgac cctcggtctg ggcctgcccg
      121 ccggcctgaa tgcctggcta cccttcctcg ttctctcctt ggcagatcgc tacacgactg
      181 tggtggagct cgacgggccg tttgagttca tctcctcgac acccgggctg atcatcatcc
      241 tgctgctcct gccgatcgag ctgattgtcg acaagattcc cggtgccgac cacatcagtg
      301 atctcgtcca caccttcgtt cggcccgttg ctggcgccat cctcgccgcc gcggtcgccg
      361 atgcctcagg cgatgttgat gcatggttag gtgccgccgt cggcggggca agcgctggcg
      421 ccacccacgc cgccaagatg tctacccgcc cggtgatcac agcctcgact ggcggagtgg
      481 gcaatccggt cgcgagcgtg gtcgaggact tcatcaccac ggtgacctcg atcatcgccg
      541 tctttctgcc actgctgctg ctcgtcgtct tgccgttggc ggccgtcggg ctttttgcgc
      601 tctggcagcg cctaaagcga ggctccgagc ggctccgggc ggcagcccgt cgctcgtcac
      661 ccacgatgcg cacatagttg cgtcgaatcg tatcaagtta tatactgcaa taggaatcat
      721 taaggcgagg aaggtgttgc gtatgactcc agtgcaattc aaggactact acgcgatcct
      781 tggcgtcgac cgtgatgcgg accagaagga gatcaagcgg gcgttccgca agaaggcacg
      841 cgagaaccac cctgatgtca accgcgacga tccggccgcc gagtcgcgtt tcaaggacat
      901 caacgaggcc tacgaggtgc tgtcgaacac cgagaagcgg cagatgtatg accgctacgg
      961 ggcagaatgg cagcgatacc gtgacgccgg gttcgatgcg agcggaccgg caccggatgc
     1021 gggcccgcag tatggatcga ccggcgactt cgaacagtgg tttaccggcg acactggcgg
     1081 gttcacgcgc accacaacgt cgggtggcgc cggcgggggc ttctccgatt tcttcaatct
     1141 gctcttcggg caggatggcg gaaggggcgg cttccgccag gcggtccggc cagtccgggg
     1201 gcaggatctc gaggtggccg ccacagtgac gctcgacgag gcctaccatg gtaccacccg
     1261 gcgcctgacc gtgcgcgcgc cgaagccgtg cgacctgtgt gacggaacgg gcgtcgtgcg
     1321 cggcgcgatg tgtccccgct gtgacggatc cggcgagatc ctggagccgc gcacgctcga
     1381 ggtgaagatt ccggctggcg tccgcactgg atcgcgcatt cgggtgcgcg gccaaggtgg
     1441 ccccggccag aacggcggcg cctcgggtga cgtctacctg caggtgaccg tcgccgatca
     1501 cccgcgcttc gagcggacag gtgatcatct gcgaaccacc gtgaacgtgc cgctctacga
     1561 cgctattctc ggcggcgagg tgcaggtgcc gaccatgacc ggcggcgtga tgctcaccat
     1621 cccgccggaa acgcagagcg gcaaggtgtt ccggctgcgc ggcaagggca tgccaaagct
     1681 cggcaagccg aaccagcatg gcgacctgct cgtgacggtg gaggtaacga tccccaccga
     1741 gctgagcgag gaggagaagc gccgtttcac cgagctcagg aatctgcgac aataaatcaa
     1801 cggacggccg ggaatgctct ggtcgtccgt tttccatgtt ctctcgcgca acgagcagtg
     1861 acgggcggag gcgatgacgg caacaatcat catgggcggc cagtggggcg acgagggcaa
     1921 gggcaagctg accgacgctc tcgcctcggc tgtgagcatg gtcgttcgtg ccaacggagg
     1981 cagtaacgcc ggccacacgg tgaagaacga ccaagggacc ttcaagctgc acctcgtgcc
     2041 gtcgggcatc ctcaacccga actgtctctg cgttattggg gccggtgtgg tcgtcgagcc
     2101 cgatacgctc atccaagagc tggatgagct tgagcagcgt ggcatcgaca cctcgcgcct
     2161 gcgcatctcc gaccgcgccc acatcgtcat gccctaccac ccagtcctcg accgccttga
     2221 ggagcagcgt cgcgaggacc tgagcatcgg gaccacgttg cggggcaacg gacccgccta
     2281 tgcggacaag atgtcgcggc atggaatccg catcggcgac ctgctcagtg gcaatgtgct
     2341 caggagccgc gtgcgttggg aggtggacca aaagaacgag atcatctccc gcctttaccg
     2401 tgccgagcca ctcgacgcca gcgagatcct gcacaaggcg gagtcatggg ccgagcgact
     2461 cgcgcggtac atccgtccca cgcaggtcga cgtgcaggac gcgctctcgg ccggcgagca
     2521 tgtgctcatc gagtgcgcgc agggcgccat gctcgacatc gattacggca cctacccctt
     2581 tgtcacctcg tcgtccccca cggcggcagg cgcctgccag ggtgccggag tcgccccgcg
     2641 tcaagtggag cgcgtcgtgg ccgtctacaa ggcgtacagt acccgcgtcg gtgccggccc
     2701 gatgccctcc gagttgaagg acgaaaccgg cgagaggatt cgccagcgag gaaacgagtt
     2761 cggcaccacc actggccggc ctcgacggac gggatggttc gacgaggtgg tcgcgcggca
     2821 gtcggcccgg ctcaacggcg tcgacgaggt tgccctcacg ctgctcgacg tgctcgatga
     2881 cttcgagacc atcaagacct gcacggccta ccgcaggggc agcgacgtga tcagtcatct
     2941 gccggccgtg ctgaacgacg ctgagaacgc cgagccagtc tggcaggagc acgtgggatg
     3001 gcagacggac accactggtg cccgagcatg ggatgatcta cctcctgctg ccaagtccta
     3061 tgtccagcac atcgggtcgg tgctcaacgc tcgcgttcgg tacatcggca ttgggccggc
     3121 tcgcgagcaa ctgatcgatc gcgggcccgt gtaaggcgcc gcctagaagc cctcgaggag
     3181 cgccatctcg gatgtcgcat cgcgggccat caggtccgcg attgctgttt ggggagagac
     3241 accctcgaag aggatacgtg cgatctgggc agcgatgggc agctcgacgc ccttcacctg
     3301 cgccagatcc agtaccgcgc gcgtggtctg caccccctca gcgacctctc tcatgttcgc
     3361 gagtactgac tcaagcgacc ggcccttggc gattccccgg ccgaccgtgt ggtttcgaga
     3421 ccgttcgcta ttgcaggtgg ccatcagatc gcctatgccg gctagcccgg caaaggtcat
     3481 tacgtctgcg ccgcaggcca acccgagccg cgccatctcg gcgattccgc gggtgacgta
     3541 cgccgccttg gcattctgcc cggcatcgag cccgtcggag atgccagccc cgatggcgat
     3601 cacattcttg aacgcaccgg caatctccac gccgatgacg tcacgggagg tgtagatgcg
     3661 gaaccgcgac gacatcaacg ccgtgcgagc gaacccggcc gtctcctccg actcggaggc
     3721 aatcaccgct gcagcaggca atccggtggc tacctcgcct gcgagattcg gtcccgatag
     3781 cgccgcgacg ggcgagccgg tcccgcgcac ggacgcaacc cgtgcggccg gcgttttcag
     3841 tgatccagga tcgatcccct tcgtggcagt gaccacgagc cctcggtacc cggccgcctc
     3901 gatctcacct gctgctgcgt ctactgcgct tgacggcacc acgacgagga ccatgtctgg
     3961 cgcttcagcg aggccctctg caacgctcga aacaacagct acggcatccg gaaggcgata
     4021 gccagctacg gaaacagggt gtcggcgctc atcgcggagc gaggtgagga cggccgggtt
     4081 ccgcgcgacc agagtgacct cgctccccgc atccactgca acgccgctga gcgtcgttcc
     4141 ccacgcgccg gagccgacga ccgtcacctt caccggacgc ctccttcata gccatgctgc
     4201 ggatttcggt acaatcctcc acagatcaag tctattcgat acctcccaac ctctggagcg
     4261 actcacatgg
//
LOCUS       DASYWL010000248         5252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_425269, whole genome shotgun sequence.
ACCESSION   DASYWL010000248 DASYWL010000000
VERSION     DASYWL010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5252
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425269"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..1351)
                     /gene="lysA"
                     /locus_tag="VGR22_09080"
     CDS             complement(5..1351)
                     /gene="lysA"
                     /locus_tag="VGR22_09080"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEV2128758.1"
                     /translation="MSDSMNTTLWPVDTHRSETGGIAIAGHSLDDLASEYGTPLYVFS
                     EAELRGNCREVLSAFRDAWPNTRVVYAGKAGLNGTLIQVVTEEGLGLDVVSSGELAIT
                     LAAGVHPAEISFHGNNKGPDELRGAVRAGVGKIVLDNLDEIDRIAAIAKEEGTTTSAL
                     VRLNPGVDVHTHRNISTGITDSKFGFPIANGQARDAVERLLAYPHIDLLGYHTHIGSQ
                     LFDSDAYVAAIDEAVAFGAEMRRDHGIEMRHLSPGGGFGIAYLDEDEPKPANYWAAVI
                     TGALRDALTRHDMPDPVLTIEPGRSVAGPAGVAVYRVGSIKEIPGTRTYVAVDGGMAD
                     NIRPALYEARYTAAVANRHPVVAGSRDVAVVGKYCESGDILIGHVSLPDVRVNDLLAI
                     PACGAYCLPMASNYNGAFKPAVIMVGPSGVRVMKKRETREDLFRNDPVLEAVAARS"
     gene            1692..3668
                     /locus_tag="VGR22_09085"
     CDS             1692..3668
                     /locus_tag="VGR22_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV2128759.1"
                     /translation="MPPVPILTVNDLGKSYGAEEIFSRVTFQIVEREHVALVGVNGAG
                     KSTVLRIIAGIEPPNEGTIAPLKGLRITYLPQEASFTSDKTLREEARLAFTPVLDMQT
                     RMRDIERQMASAGDDHLEAIMDEYARLQTRFETAGGYDIEHRTDEVLNGLGFSEEQFD
                     QPVAQLSGGQKTRVALAKALLADPDLLLLDEPTNHLDLEMLEWLEDFLRSWNGACLIV
                     SHDRYFLDRVTSRTMDLSFGRLEDYPAPYGRFLKLREERTERRWKEFQEQQEYIARQE
                     EFIKKYGAGQRYREARGRQKKLDRLERIERPQEHDRLAIRIGSATRSGRMVLSTTEMS
                     IGYEEDSETGQPVELLRTPELEVELGDRIGLLGPNGSGKTTLLKTLMGILPALEGDYH
                     LGTNVHPGYYAQSHEQLRGRGEGTPLSVILNVQPMSEEAARTYLGRFLFSGDDVYKQV
                     ASLSGGERSRLALAVLLLERANFLVLDEPTNHLDINARESLEHMLTGFDGTILFVSHD
                     RYFIDKVATKLWVVEDGTITEQLGNYTDFHRRLEAAPPPAEPKPEPKPEPIASPTGDA
                     MLTVSSKGKPRRKTSADVEKRLTKVEREISQLEGKVNELSDALAIASADQDLDAVARL
                     GEEYERLLNELEDTYQRWEAITEERELVAAEAGE"
     gene            3665..4282
                     /gene="coaE"
                     /locus_tag="VGR22_09090"
     CDS             3665..4282
                     /gene="coaE"
                     /locus_tag="VGR22_09090"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEV2128760.1"
                     /translation="MTDQKFVLGVTGNIASGKSTVVRRLEEHGAVSIDADLVYRELVG
                     PGQPLLRKLADHFGEQIIAPDGNLDRTALGRIVFSDPAKLAELDRISHPAVIAEIDRR
                     VEAVEEGVVVIDAVKLIESGHADRCDEVWVVTIDTHVQMTRLKARNTLSEAEARRRIE
                     AQPPTALKLERADRVIDNSGTLEQTMEQVDAGWRAIMRATGQCAG"
     gene            4305..4961
                     /locus_tag="VGR22_09095"
     CDS             4305..4961
                     /locus_tag="VGR22_09095"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEV2128761.1"
                     /translation="MAVSPETRGGSSTLPETCKIDVYFDYACPFAWAAQMWLDQVQEE
                     LDDRLSITWRMFPLQQVNHPNPDFKIWEQPNDGSSSTLRSFQAFHAASQQGSEPFRRF
                     HEAMFCKRHEEGRNLGRQQVLEAVAEEAGLDLDRFRQDLKSDAVFADVERDWIEGRNE
                     HEVFGTPTIVFENGEAAYLKLNYMEMPEDPVEFFHEFVSIVRDRPEVIEIKRPSPMGG
                     "
BASE COUNT         1023 a         1706 c         1624 g          899 t
ORIGIN      
        1 ccgctcagga tcgagcggcg acagcttcca gcacgggatc gttccggaag agatcctcac
       61 gggtttcgcg ctttttcatc actctcacgc cggatggacc gaccatgatc actgccggct
      121 tgaaggcgcc attgtaattg ctggccatcg gcaggcaata ggcaccgcac gccgggattg
      181 ccagcaaatc gttaacgcgc acgtccggca gcgagacgtg cccgataagg atgtcgcccg
      241 attcacagta ttttcccacc accgcgacgt cacgacttcc ggcgaccacc gggtgacgat
      301 tggccacagc cgccgtgtac cgagcctcat acagtgcagg acgaatattg tcggccatcc
      361 cgccgtccac cgcaacgtaa gttcttgttc ccggaatttc cttgatcgac cccacgcggt
      421 agaccgcgac ccccgccggc ccggcaactg agcgtccggg ctcgatcgtc agcaccggat
      481 ccggcatgtc gtggcgcgtc aaggcgtcgc gcaaggcgcc ggtaatgacc gccgcccagt
      541 agtttgccgg cttcggctcg tcctcgtcga gatacgcgat gccgaacccg cctccggggg
      601 acagatgccg catttcgatg ccatggtctc gtcgcatctc ggctccgaag gcgaccgcct
      661 cgtcgatcgc ggcgacgtac gcgtccgagt cgaacaactg cgagccgata tgggtgtgat
      721 agcccagcag atcgatgtgc ggatacgcca gaagtcgctc gacagcgtct cgcgcctgcc
      781 cgttggcgat cgggaaaccg aatttggagt cggtgattcc agtgctgata ttgcggtggg
      841 tgtggacatc gacgcccggg ttcaaccgca cgagcgcgga cgtcgtcgtc ccctcctctt
      901 tggcaatcgc ggcgatccgg tcgatctcat caaggttgtc gagcacgatc tttccgacac
      961 cggcacgaac ggcaccgcgc aactcgtcag gccccttgtt gttgccatga aaggagatct
     1021 cggcgggatg aacacccgcc gccaacgtga tcgccagctc cccgctggag accacgtcga
     1081 gcccgagccc ctcctcggtt accacctgga tcaacgtgcc gttgagcccc gccttgccag
     1141 catagaccac acgagtgttc ggccaggcgt cacgaaatgc tgacagcacc tcccggcagt
     1201 tgccgcgaag ctcggcttcc gagaagacgt agagtggtgt tccgtattcg ctggcgaggt
     1261 cgtcgagcga gtgcccggcg atcgcaatgc cgccggtttc gctgcggtgc gtatcgaccg
     1321 gccagagggt ggtgttcatc gaatcggaca tgcaacggcc ctgtgttcag atttgcgcgc
     1381 ggctatctct gctgagcagt gtacacagac aaggagcctc cacgcgcggc tcttcgcccg
     1441 actgggctaa gcgctcgtcg aggggttggt atacttgcag gtggaaatgg gcgtgcttcc
     1501 acagaggcgc tgcttcgcct gctcaatccc ggctggctga acacgcactt gggaattcgg
     1561 agcaacatgc tgctggtcag atgatgtcga acaaccgagg ccgccggctg gccgcgatgc
     1621 acacgcgtcg cgggcgaggt ggcgtaccgc gctccgtctg gcccacccgc ttcgttccaa
     1681 ggaaccgttc catgccaccc gtccccatcc tgactgtcaa tgacctcggc aagagctacg
     1741 gcgccgagga aatcttctct cgcgtcacct tccagatcgt cgagcgcgag catgtcgcgc
     1801 tcgtgggcgt caatggtgct ggcaagtcga ccgtgctgcg catcatcgct ggcatcgagc
     1861 ccccgaacga gggcaccatc gccccgctca aaggtctgcg catcacctac ctgccgcaag
     1921 aggcgtcgtt cacctcagac aagacgctcc gcgaggaagc acgcctcgcg ttcacccctg
     1981 tgctggacat gcagacgcgc atgcgggaca tcgagagaca gatggcctcg gccggggatg
     2041 accacctcga agcaatcatg gacgagtatg ctcgcctgca gacccgcttc gagactgcag
     2101 gtggctacga cattgaacac cggaccgacg aggtcctgaa cggtctcggg ttctccgagg
     2161 aacagttcga ccagccggtc gcccaactca gcggcggcca gaagacgcgc gtcgcgctcg
     2221 ccaaggcgct gctcgccgac cccgacctgc tgctgctcga cgagccgaca aaccacctgg
     2281 atctcgagat gctggagtgg ctcgaggact tcctgcgctc ctggaacggc gcctgtctga
     2341 tcgtctccca cgaccggtac ttcctcgatc gcgttaccag ccgaaccatg gatctctcct
     2401 tcggccgtct ggaggactac cccgctccct acggtcgctt cctgaagctc cgcgaggagc
     2461 gcacggaacg cagatggaag gagtttcagg agcaacagga gtacatcgcc cggcaggagg
     2521 agttcatcaa gaagtacggt gccgggcagc gctatcgcga ggcccgcgga cggcagaaga
     2581 aactcgaccg gctggagcgc atcgagcgac cgcaagagca cgaccgcctc gccatcagga
     2641 tcggcagcgc cacccgcagc ggccggatgg tcctctctac gaccgagatg tcgatcggct
     2701 acgaggagga cagcgagacg ggccagccgg tggagctgct gcgcacgcca gagctggaag
     2761 tcgagctcgg cgatcggatc gggctgctcg ggccgaacgg cagcggcaag accaccctgc
     2821 tcaagacgct gatgggcatc ctgccggcat tggagggcga ctaccatctg ggaaccaacg
     2881 tccaccccgg ctactatgcc cagagccatg agcagcttcg cggccgggga gagggcaccc
     2941 cgctctcggt gatacttaat gtccagccga tgagcgagga ggcagcgcgc acctacctcg
     3001 ggcgcttcct gttcagcggc gacgacgtct acaagcaggt cgcctcgctc tccggtggcg
     3061 aacgatcacg gctggcactt gcggtactgc tgctggagcg cgccaacttc ctggtgctgg
     3121 acgagccgac caatcacctc gacatcaatg ctcgcgagtc gctggagcac atgctgacgg
     3181 gctttgacgg cacgatcctg ttcgtttcac acgaccgcta cttcatcgac aaggtcgcga
     3241 cgaagctgtg ggttgtcgaa gacggcacca tcaccgagca gctcggcaac tacaccgact
     3301 tccatcgacg gctggaggcg gcgccccccc cggcggagcc gaagcccgag cccaagccgg
     3361 agccgatcgc atcgccaacc ggtgatgcca tgttgaccgt cagtagcaag ggcaagcctc
     3421 gccgcaagac ctcggcagat gtcgagaagc ggctgacgaa ggtcgagcgc gagatctcgc
     3481 agctggaggg aaaggtcaac gagctgtcgg atgccctcgc gattgccagc gccgatcagg
     3541 acctcgacgc ggtcgcccgc ctcggagagg agtacgagcg cctgctgaac gagctggagg
     3601 acacctacca gcgctgggag gcaatcaccg aggagcggga gctggtggcc gccgaggccg
     3661 gcgaatgacg gatcagaagt tcgtcctagg cgtcaccggc aacatcgcct ccggcaagag
     3721 cacggtggtc cgtcgcctcg aggagcacgg ggcggtcagc atcgacgccg atctcgtcta
     3781 ccgggaactc gtcggtcccg gacaaccgct ccttcgcaag ctcgccgacc acttcggcga
     3841 gcagatcatc gcccctgacg gcaacctcga ccgcaccgcg ctcggccgga tcgttttcag
     3901 cgaccccgcc aagctcgccg agctggaccg cattagccat cccgccgtga tcgccgagat
     3961 cgatcgccgc gtcgaggcgg tcgaggaggg tgtagtcgtg atcgatgcgg tcaaactcat
     4021 cgaaagcggc cacgccgatc gctgcgacga ggtatgggtg gtgacgatcg atacgcacgt
     4081 gcaaatgaca cggctcaagg cgcggaatac actctctgaa gcggaagcac gccgccgcat
     4141 cgaggctcaa ccgccaacgg cgctgaaact cgagcgtgcg gaccgtgtca tcgacaacag
     4201 tgggacattg gaacaaacca tggagcaggt cgacgccggc tggcgggcga tcatgcgtgc
     4261 cacgggccag tgtgccggct gatattcacg aaagggtctc aagcttggcg gtttctcctg
     4321 agacgcgcgg cggcagctca acactgccag aaacgtgcaa gatcgatgtc tacttcgact
     4381 atgcatgccc gtttgcctgg gctgcccaga tgtggctcga ccaggtgcag gaggagttgg
     4441 acgaccgact ctccatcact tggcggatgt tccctctgca gcaggtgaac caccccaacc
     4501 cggacttcaa aatctgggaa caaccgaacg acggctcaag cagcacactg cgctccttcc
     4561 aagcattcca cgccgccagt cagcagggta gcgaaccgtt ccgccgtttc cacgaggcga
     4621 tgttctgcaa gcgccacgag gagggtcgca acctcggccg tcagcaggtg cttgaggcag
     4681 tcgccgaaga ggccggtctc gatctcgacc gctttcggca ggatctcaag tcggacgctg
     4741 tgttcgcgga tgtcgagcgc gactggatcg agggacgcaa cgagcacgag gtcttcggca
     4801 cgccgacgat cgtcttcgag aatggcgagg ctgcttacct caagctgaac tacatggaga
     4861 tgccggaaga cccggtggag ttcttccacg aattcgtgag cattgtgcgt gaccgtcccg
     4921 aggtcatcga gatcaagcgc ccctccccga tgggtgggta ggcgaggctt gatcctatcg
     4981 ttcgctcctg tcatttcgag ccggagcccg gcgtagcccg attcgtggcg agaaatctct
     5041 tccgcccaga acatcgactc ctgccaggca agagatttct cgactgcagg ctcgcgagcg
     5101 gtcaccttcc gctcgaaaat gacagccttc cacatcggtt cgagccgtga accatgcctt
     5161 tcccaagtga cattgcccaa tgagggaatc cgcgctgcca cctgccaaag agattgcgtc
     5221 gttccgcgag gccctgctcg gctggtggga gg
//
LOCUS       DASYWL010000249         7351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_219155, whole genome shotgun sequence.
ACCESSION   DASYWL010000249 DASYWL010000000
VERSION     DASYWL010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7351
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219155"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..702)
                     /locus_tag="VGR22_09100"
     CDS             complement(37..702)
                     /locus_tag="VGR22_09100"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEV2128762.1"
                     /translation="MTVVILASVVAGILFDALFGQLISLRPDAIGDWLDDLGPWAPLM
                     FIILLAAAVVVSPIPSVPLDIAAGLTFGLLWGTVYVLIGAELGAIIAFATARRIGRPR
                     LGRRLPAVAMTQIDAFASRCGVRALLLMRLLPVFNFDWVSYAAGLTLISFPAFAFATF
                     VGMVPPVVAIVAVGATCSVSPVLAGTIFTLLVLIVVIPLALPWFFLARRDRNEAWYDG
                     VAQ"
     gene            complement(952..2391)
                     /locus_tag="VGR22_09105"
     CDS             complement(952..2391)
                     /locus_tag="VGR22_09105"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HEV2128763.1"
                     /translation="MNRQDTFDVIVIGAGQGGGPIASTFARDGRETALIEREYAGGSC
                     VNWGCTPTKTMIASGRVAHLARRAESYGVHTGEIAIDMTTVRQRKWDIVEMFRQGSRS
                     GIDSTEGLEYIEGEARFTGEKTIEVTLGDGGRRSLCAGTIVLDVGDRSRPLGIDNPGG
                     VEILDNRSVMELGGVPDHLVVVGGGYVGLEFGQLFRRLGAEVTIVHRSEQLLSREDPD
                     IAEAVAEILREDGLALELNASPVAATRDGDGIIVAIERKDGSRREIHASHLLNAAGRM
                     PNTDALDIENTGLSLDEKGYIRTNDQLETAVPGIYAIGDIRPGPKFTHISYDDYRILQ
                     ANLVDGGDRSIDDRQIPYTAFIDPQLGRIGFTEGQAKEQGIPYLVGKLPMSSVARAIE
                     TDETRGLMKVLVHAETERILGAAVLGIEGGEIAAMLQIAMMGNLPYTALRDGVFAHPV
                     DPVGGWHIVRPAIERRRGKPALDTGDASC"
     gene            complement(2443..2682)
                     /locus_tag="VGR22_09110"
     CDS             complement(2443..2682)
                     /locus_tag="VGR22_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128764.1"
                     /translation="MSITQIAEITAIIKPSRSLFVPHPFGLTFGTVNDAATQRAVLAA
                     LLDTAATMPAAGMLDSGFTWEWDDLRTRQLRKQRH"
     gene            complement(2746..3276)
                     /locus_tag="VGR22_09115"
     CDS             complement(2746..3276)
                     /locus_tag="VGR22_09115"
                     /inference="COORDINATES: protein motif:HMM:NF019003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HEV2128765.1"
                     /translation="MAQEPHHSEVNDSERGGVNFADIERSWVQGRLYPDFGWRAFDRF
                     SPVNPMRVPLEESRVAFVTTAGAHLADQPPFNLGTTGDPSFRAFASNTPLDDLVLTHG
                     GYNTRRTTEDKNAVLLLDHLRHAAATGVIGSLAATVYSFMGYVANTAPLMQESGPAVA
                     RRLIDDQVDLVLLAPT"
     gene            complement(3396..3935)
                     /locus_tag="VGR22_09120"
     CDS             complement(3396..3935)
                     /locus_tag="VGR22_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128766.1"
                     /translation="MNKGGTLLWGFVATVVLTGLMAATQRLGVTRMNIPFMLGTMVTP
                     NRDRAKLIGFGMHFMNGWLFASIYTAAFRSWRRSTWWLGAGIGLVHGLFVLVAGMPLL
                     PSMHPRMASEEQGPTPTRMLEPPGVLALNYGKRTPVSVLVAHILYGGILGAFIRVGSP
                     GWLARRGVSKTVRRLPPQR"
     gene            complement(3932..5815)
                     /locus_tag="VGR22_09125"
     CDS             complement(3932..5815)
                     /locus_tag="VGR22_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEV2128767.1"
                     /translation="MISTRTTERYPPIGDYAIIGNCRSAALVSREGSMDWLCWPRFDS
                     PSIFAALLDAEQGGCFSIRPTGAFRTERRYLPDTNVIETVFHTAHGSLALRDVMVVMA
                     EGDKHNALGAEHEILRELEGLTGEVEVELHYEPRPDYARRAVSLESRDQLGVWCHAGA
                     AALVLRGDVSLAIAADKRSANSTIGMRAGERRYLSLSYSSDGPAVIPALGSVADARFA
                     QSIQWWQSWANRCTYDGPYREAVVRSLLALKLMSFAPSGAVIAAPTTSLPEKIGGQRN
                     WDYRYCWLRDASFTVRALLELGYQDEAGAFCGWLLHATSLTWPELQVVYDVFGKARLP
                     EDELDHFEGYAGSRPVRIGNGAHDQIQMDVYGEVLDAISRLANRPDFFDSDTLRFLNG
                     LGRTVCDQWREPDAGIWEGRGEPQHYTHSKVLCWIALDRLIKLHETHGVDIDVDRFVV
                     EREAIRAEVERRGYNEQLGAYTQVFDGDALDASLLTLPWYGYIDGTHPRMLATLERIR
                     ERLGHAALIYRYADDTDDGFPSGEGAFGVCGFWAVDCQVRCGHIAEATEAFEQLIGYA
                     NDVGLYAEETDPGTGAALGNFPQAFTHLGLINAALTLAGDGRTPGAIGGAERQVSGKA
                     DTR"
     gene            complement(5812..6639)
                     /locus_tag="VGR22_09130"
     CDS             complement(5812..6639)
                     /locus_tag="VGR22_09130"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV2128768.1"
                     /translation="MTTETDPQIVVVTGASSGVGRAIAHAFGQRGAHVVPIAPATRRL
                     CRQPLRRSVRPEAMPSCCPWTWRTALRRMRPRSQETIIQIGSALAYRSIPLQSAYCAA
                     KAAIRGFTDSLRTELIHEGSNIRVTMLQLPAVNTPQSLRQRNKLPQLAQPVPPLFAPE
                     AIAGVVVWAADHAPREMLIGRPSLQTVWAQKFFPSLLDLYLGKTGYTPQMVDDANNQN
                     GRDILFETLSEDPGARGPYNDKEQGPDLQMRLRPHARPLSAAFSIASSAFKAIGRRA"
     gene            7018..7311
                     /locus_tag="VGR22_09135"
     CDS             7018..7311
                     /locus_tag="VGR22_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128769.1"
                     /translation="METATMPCTLTHRSLDIYTAEACFGCDRARELAAQVRGWRLPHV
                     TVTLRDLGDPGTDRPDRVFAVPTYLLDGRVISLGNPDVSWLYQLLTSEAEDGA"
BASE COUNT         1296 a         2403 c         2342 g         1310 t
ORIGIN      
        1 gttccaccac cgatcacgat gagatcgaag gtcgagctat tgagcaacgc cgtcatacca
       61 cgcctcgttt cgatcacgcc ttgcgaggaa aaaccatggc agagccaagg gaatcaccac
      121 gatcaggaca aggagggtga agatcgtccc agccagaact ggactcacgg aacaggtggc
      181 cccgaccgcg acaatcgcga ccaccggggg aaccatgccc acgaacgtcg cgaacgcgaa
      241 tgcggggaac gagattaatg tcagccctgc ggcatagctg acccaatcaa aattgaagac
      301 cgggagcagt cgcatcaaaa gcagcgctcg tactccacat cgcgacgcga acgcgtctat
      361 ctgggtcatc gccactgctg gtagccggcg gccgagccga ggacggccga tcctccgtgc
      421 agtggcaaag gcgataatgg ctcccagctc ggcaccgatc aggacataca cggtgcccca
      481 aagaagaccg aacgtgagtc cggcggcaat atccaggggc acggagggaa ttggcgagac
      541 cacaaccgcc gctgccagca ggatgataaa catcagcggc gcccacgggc cgagatcgtc
      601 gagccagtca ccgatggcgt ccggccgaag tgagatcagc tgcccgaaga gtgcatcgaa
      661 gagaattccg gcgacgacgc ttgccaggat aacgacggtc acccacgttc gtcattgcca
      721 ccgggtaacg gtttgcacga cacggccgct gacggacgtt tcctcgatca tcgcgatttc
      781 tccaagagcg gcgcggcacg gttcggtgtg gacgtgtccc cgatctcgcg cttcacgaaa
      841 gccaacacga gcactcgctg ccggtgatcc cgtctctttc ttcgcgtgat gacatacaga
      901 agccggtctc gtggtcgacc atcgccccga aggtgctgcc ctgtcaccgc ctcaacagct
      961 tgcatcaccc gtatcgagtg cgggcttacc acgccggcgt tcgatcgctg gtcgcactat
     1021 atgccacccg ccaaccgggt ctaccgggtg ggcgaagaca ccgtcgcgca gggcggtgta
     1081 cggaagattg cccatcatcg cgatctggag catcgccgcg atctcgcccc cctcgatgcc
     1141 gagcaccgcc gcgccgagga tccgctcggt ctcggcgtgg acgagcacct tcatcagccc
     1201 gcgcgtctcg tcggtctcga tcgcccgggc gacgctgctc atcggcagct tcccgacgag
     1261 ataggggatg ccctgctcct tcgcctgccc ctcggtgaag ccgatgcggc cgagctgcgg
     1321 gtcgatgaac gcggtgtacg ggatctggcg gtcgtcgatc gagcggtcgc cgccgtcgac
     1381 caggttcgcc tgcaggatgc gatagtcgtc gtaggagatg tgcgtgaact tcgggccggg
     1441 ccggatgtcg ccgatcgcgt agatgcccgg cacggcggtt tcgagctgat cgttcgtccg
     1501 gatatagccc ttctcgtcga gggagagccc ggtgttctcg atgtcgagcg cgtcggtgtt
     1561 aggcatacgg ccggcggcgt tgagcagatg ggaggcatgg atctcgcgcc gggagccatc
     1621 cttccgctcg atggcgacga taatgccgtc gccatccctc gtggccgcca cgggcgaggc
     1681 gttcagttca agcgcgaggc cgtcctcccg caggatctcc gcgacggcct ccgcgatgtc
     1741 cgggtcctcg cgcgagagca gttgttcgct gcggtggacg attgttacct ctgcgccgag
     1801 ccgccggaac agctgtccga actcgagccc aacgtagccg ccaccgacca ccaccaagtg
     1861 gtccgggacc ccgcccagct ccatgaccga gcggttgtcg aggatctcga cgccgcccgg
     1921 gttgtcgatg cccagcgggc gcgaccggtc cccgacatcg aggacgatcg tccccgcgca
     1981 cagcgaccgc ctgcccccgt caccgagcgt cacctcgatc gtcttctcgc cggtgaagcg
     2041 ggcttcgccc tcgatgtact cgagtccctc cgtgctgtcg atgccagagc ggctgccctg
     2101 tcggaacatc tcgacgatat cccacttgcg ctggcgaacc gtggtcatgt cgatggcgat
     2161 ctcgccggtg tgcacgccgt agctctcggc gcgacgggca aggtgggcga cgcggccgga
     2221 ggcgatcatc gtcttggtcg gggtgcagcc ccagttgacg cagctgccgc cggcatattc
     2281 gcgttcgatc agcgcggtct ctcggccgtc gcgggcgaag gtgctggcga tgggcccgcc
     2341 gccttggccc gctccgataa cgatgacgtc gaaggtgtcc tgccggttca ttggcgtgct
     2401 cctgtcattc cgggtggatt cccgatcggc tcctcgagag tgctagtgcc gttgcttgcg
     2461 gagctgtcgg gtgcggaggt catcccactc ccaagtgaag ccgctgtcca gcattcccgc
     2521 tgctggcatg gtcgccgccg tgtcgagcag ggccgccagg accgcgcgtt gcgtggccgc
     2581 atcgttgaca gtgccgaagg tcaggccaaa cggatgcggg acgaagaggc tccgtgacgg
     2641 cttgatgatc gccgtgatct ccgcgatttg cgtaatcgac atcgtgcgga gccccgcgtc
     2701 gtccaggatc cgttggagca ggcccacgga ctgctggcag acgggtcatg tcggcgccag
     2761 gaggaccagg tcgacctggt catcgatgag gcgccgagcc accgccgggc cgctctcctg
     2821 catgagcgga gccgtgttcg ccacataccc catgaaggag tagaccgtcg ccgccaatga
     2881 accgatcact ccggttgctg ctgcgtggcg caggtggtcg agcagcagca ctgcgttctt
     2941 gtcctcggtg gtgcgtcggg tgttgtatcc gccgtgggtc aagaccagat cgtcaagcgg
     3001 tgtgttactc gcaaacgcgc ggaagcttgg atctccggtc gtgcccagat tgaatggcgg
     3061 ctggtcggcg aggtgtgcgc ccgccgtggt gacgaatgcg acgcggcttt cctcgagtgg
     3121 gacgcgcatc ggattgaccg gtgagaaccg gtcgaacgcg cgccagccaa agtcggggta
     3181 cagacgcccc tgaacccagg atcgctcgat gtcggcgaag ttgaccccac cgcgctcgct
     3241 gtcattcact tccgaatgat gaggctcctg tgccacgcat acgctcctta tcgatggtca
     3301 gataacgagg cggccaggga ctgcgcatgc gctcgcgact gccaccgcac gattgcgccg
     3361 catcctcgat tcttttgcgg gccggccgca gcgggttacc gctgcggcgg gagtcgccgc
     3421 acggtcttgc taactcccct gcgtgcgagc cacccgggcg atcccacccg gatgaatgcc
     3481 cccaggatgc cgccgtagag tatgtgggca acgagcaccg acacgggtgt tcgcttgccg
     3541 tagttgagcg ccagaacgcc gggcggttcc agcatgcggg tcggggtcgg tccctgctcc
     3601 tcgctggcca tgcgcgggtg catgctcggc agcagcggca tccctgccac gaggacgaag
     3661 aggccgtgca ccaggccgat ccccgcgccg agccaccagg tgctgcgccg ccagctgcga
     3721 aacgcggcgg tgtagatgct ggcgaaaagc cagccgttca tgaagtgcat cccgaagccg
     3781 atcagcttcg cccggtcacg gttcggggtc accatggtgc ccagcatgaa ggggatgttc
     3841 atcctggtca cgccgagtcg ctgggttgcc gccatcaggc cggtcaggac caccgtggcc
     3901 acgaatcccc agaggagtgt gccgcccttg ttcatcgggt gtctgccttt ccggatacct
     3961 gacgctcagc cccaccgatc gcgccagggg tacgaccgtc gccggcgagg gtcagcgcgg
     4021 cattgatcaa tcccagatgc gtgaaggcct gcgggaagtt cccgagggcc gccccggttc
     4081 ctgggtcagt ctcttccgcg taaagcccga catcgttcgc gtagccgatg agttgctcga
     4141 aggcctctgt cgcctcggcg atgtgcccgc accgcacttg gcaatctacc gcccagaagc
     4201 cacagacgcc gaacgccccc tcgcccgagg ggaacccgtc gtcggtatcg tcagcatacc
     4261 gatagatcaa cgccgcatgg ccgagtcgct cgcggatccg ctccagagtc gccagcatgc
     4321 gtggatgtgt gccatcgata tagccatacc aaggcaaggt gagcaggctg gcatccagcg
     4381 cgtccccgtc aaacacctgc gtgtaggcgc caagctgctc gttgtatccc cggcgctcca
     4441 cctcggcccg gatcgcctcc cgctcgacca cgaaccgatc aacatcaatg tcaactccgt
     4501 gcgtctcatg cagcttaatg aggcgatcaa gcgcgatcca gcagagcact ttcgaatgcg
     4561 tgtagtgctg cggctcgcct cggccctccc agatcccggc atccggctcg cgccattggt
     4621 cgcaaaccgt ccggcccagc ccgttcagga agcgaagggt gtcgctgtcg aaaaagtcag
     4681 gacgattcgc tagtcgggag atggcgtcga gcacttctcc gtagacgtcc atctggattt
     4741 ggtcatgggc gccgttccca atacggaccg ggcgtgaccc ggcatacccc tcgaagtgat
     4801 ccagttcatc ctccggcagc cgcgccttgc cgaaaacgtc atagaccacc tggagctcgg
     4861 gccaggtcaa acttgtcgca tggagcagcc acccgcagaa agccccggct tcgtcctggt
     4921 aaccgagttc gagcagggcg cggacggtaa acgacgcgtc acgcagccag caataccggt
     4981 aatcccagtt tcgctgaccg ccgatctttt caggaagtga tgtcgttggt gcggcgatca
     5041 ccgcgccgga cggggcgaag ctcatcagtt tcagcgcaag gaggctccgc acgaccgcct
     5101 cgcggtaggg cccgtcgtac gtgcagcggt ttgcccagga ttgccaccat tggatcgatt
     5161 gcgcgaatcg tgcatcggcc accgatccga gtgcggggat cactgccgga ccgtcgctgc
     5221 tgtaggagag cgaaagatac cggcgctccc cggcgcgcat gccaatagtg gagttggccg
     5281 accgcttgtc tgcggcgatg gcgagtgaca cgtcaccgcg caggaccagg gccgctgcgc
     5341 cagcgtggca ccacacccca agttgatcac gtgactcaag ggaaacggcc cgccgggcgt
     5401 agtcgggacg cggctcgtag tgaagctcta cctcaacctc gccggtcaag ccttcaagct
     5461 cgcgcaggat ctcgtgctcg gcaccgagcg cgttgtgctt atccccctcg gccatcacca
     5521 ccatcacatc acgcagggca agtgaaccgt gagcggtatg gaagacggtt tcgatcacgt
     5581 tcgtatccgg cagatagcgc cgttccgtgc ggaacgcgcc tgtcggtcgt atggagaagc
     5641 aacctccctg ctcggcgtcg agcagcgcgg cgaagatgga cgggctgtcg aagcgcggcc
     5701 agcagagcca gtccattgag ccctcgcgtg acacgagggc agcgctccgg cagttcccga
     5761 tgatggcgta gtcgccaatg ggtgggtagc gttcggtagt tcgcgtggag atcatgcacg
     5821 tcgtccaatt gctttgaatg ccgatgaggc tatgctaaat gccgcactga gtggtcgcgc
     5881 gtgtgggcgc aggcgcatct ggagatccgg tccttgttcc ttgtcgttat agggaccgcg
     5941 tgcccctgga tcctccgaca gcgtctcgaa caggatgtct cgcccgttct gattgtttgc
     6001 gtcgtccacc atctgcggcg tgtagcccgt cttccccaga tagaggtcca gcagagacgg
     6061 gaagaacttt tgcgcccaga ccgtctgcag ggacgggcgg ccaatcaaca tttcgcgagg
     6121 ggcgtgatcg gccgcccaga cgacgacacc cgcgatggcc tcaggcgcaa acagcggcgg
     6181 cacaggctgc gccagttgtg gcagcttgtt gcgctgccgg agggactgcg gcgtgttgac
     6241 cgccggcaac tgcagcatgg tgacccggat attcgaccct tcatggatga gctccgtgcg
     6301 cagcgagtcc gtgaacccgc ggatagccgc cttggccgca cagtaggcgc tctggagcgg
     6361 gatcgagcgg taggccagcg ccgagccgat ctggatgatt gtctcttggc ttcgcggccg
     6421 catgcgccgc agcgccgtcc gccacgtcca ggggcagcac gagggcatcg cttccggcct
     6481 cacggatctc cgtagcggct gcctgcagag tctcctcgtt gcgggcgcga tcggcacgac
     6541 gtgggcaccc cgctgcccga acgcgtgggc aatggcgcgc ccgacacccg aggacgcccc
     6601 ggtgacgacg acaatctgtg gatctgtctc tgtagtcata cacaacccca aaactctcta
     6661 tctctccttg caggcaccgg tctgtccacg caggaacggc atccggcgcc cgccaacggc
     6721 gtgcatcgca gactcacagg gcagagcact cattttgctg tgtcgcttgt cctgtctcgc
     6781 tcggcatttg tggccatccc ctgtatccag ctagctctgc agatttcaag cagcggcggc
     6841 cgatcacatt cctcgcctga ctcatgggag gtgctcgaca ccgcacggtg agcggggtgc
     6901 atccccctgc cactgctctc cagtatcctc acgttctggt gtcgcgtatg taagcgggct
     6961 taccgccaat ccagcggggt ctcgctatac catgagcact acgaggagat ggaacggatg
     7021 gagactgcga ccatgccctg cacgctcact catcgatcgc tggacatcta cactgccgag
     7081 gcgtgctttg ggtgcgaccg ggcccgggag ctcgccgcgc aggtgcgcgg gtggcggctg
     7141 ccccatgtca ccgtgacgct gcgcgatctc ggcgaccccg gcacggaccg gcccgaccgc
     7201 gtctttgccg tgcccaccta cctgctggac ggccgcgtga tcagcctggg taacccggac
     7261 gtgtcatggc tctatcagct gttgacatcc gaagccgagg acggagcgta ggttcggtca
     7321 tatggaggta tgccatggcc aaacacgcac c
//
LOCUS       DASYWL010000250         1915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_312462, whole genome shotgun sequence.
ACCESSION   DASYWL010000250 DASYWL010000000
VERSION     DASYWL010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312462"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(388..>1915)
                     /locus_tag="VGR22_09140"
     CDS             complement(388..>1915)
                     /locus_tag="VGR22_09140"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HEV2128770.1"
                     /translation="RLDYLASLGVTCLWLLPFFPTPNHDNGYDIIDYYGVDERHGSLG
                     DCVAFLHDARARGLEVIIDLVLHHTSDQHPWFQQSREGGPYRDFYVWTDDPPDETAAE
                     VIFPGVEQGIWARDEQAGAWYLHHFYSFEPDLNIEHPAVRREIQKIVGMWLQLGVSGF
                     RVDAASRMLGREALLPQAEQQPHEFLRYLRSIVSARDGRAALIAETDVEVERLATYFG
                     DGDQMQLLLNFWLDNHLFLALAREEAEPIVRAFRAMPEKPSVGQWANFVRNLDELDLE
                     RLSVEEQEEVYAVFAPDEEMRIYRRGIRRRLAPMLGGDRRRLELVYSLLFSLPGTPVL
                     VYGDEIGMGDDLALPERWSVRTPMQWSCDANAGFSDAPEDAVVRPVVSEGEFGYQHVN
                     VVDQRRDPDSLLNWMQRVIRMRRQNPEIGWGELTLIETDQPNVLAHRCDSEGGTLMTV
                     HNLSGASCTATLSPGDLDLAAAVEIFGDHDYGPVQEELPSIRLDAYGYRWFRCGGVQL
                     "
BASE COUNT          357 a          629 c          613 g          316 t
ORIGIN      
        1 gatgaccgga ccgcaggcct cctatctgca gacattgctg cagcaggccg gcaagtcgga
       61 ggatgacctt aatcccaaca tgaccaagcg cgaagcctcc gagctgatcg acaggctgca
      121 ggaggagacg gggcgcggcc agggctccgg cggcgacaat tcctgatccc caacggtgcg
      181 caggttgact ggttggccgg gtaggggccg acctcccgca gcgagttgtc ggctctgccg
      241 cccccagata atgcccccgg cgaaccactc cctatggagg cccgcttgtc tgcgtcaacg
      301 tcactcactg caaaccgctc tgacggtgtc gcacgtcatc tctgggcaac gatcatggag
      361 acgacgagct gtccgtcccc acgtgaccta cagctgcacg ccaccacatc ggaaccagcg
      421 gtatccgtac gcatcgagcc ggatcgacgg caactcctcc tgcactggcc cgtaatcgtg
      481 atcgccgaaa atctcgacgg cggcggcaag gtctagatcg ccaggcgaga gcgtcgccgt
      541 gcaggatgcc ccggagaggt tgtggaccgt catcaatgtg ccaccttccg agtcacaccg
      601 atgggcgagc acgttgggct ggtcagtctc gatcagcgtc agctcgcccc agccgatctc
      661 cgggttctgc cggcgcatcc ggatcacgcg ctgcatccag ttgagcaacg agtcggggtc
      721 ccgccgctgg tcgacgacat tgacgtgctg atagccgaac tccccctcgc tgacgaccgg
      781 ccgcacgacc gcgtcctccg gtgcatccga gaagccagcg tttgcatcgc acgaccattg
      841 catgggtgtg cgaacgctcc accgctccgg cagggcgagg tcatcgccca tgccgatctc
      901 gtcgccgtag accagcaccg gcgtgccggg caggctgaac agcaggctgt agacgagctc
      961 cagccggcga cggtcgccac ccagcatcgg ggcgagtcga cgccggatgc cgcgacggta
     1021 gatccgcatc tcctcgtcag gtgcgaacac cgcgtagacc tcctcctgct cctccactga
     1081 caggcgctcg aggtcgagct cgtcgaggtt acgcacgaag ttggcccatt gaccgacgga
     1141 gggcttctcc ggcatggcgc ggaacgctcg cacgatcggc tcggcctcct ctcgggcgag
     1201 cgcgaggaag agatggttgt cgagccagaa gttgagcaga agctgcatct ggtcgccatc
     1261 accgaagtag gtggcgagcc gctcgacctc gacgtccgtc tcggcaatga gtgccgccct
     1321 gccatcacgc gcgctgacga tgctgcgcag gtagcggagg aactcgtgcg gctgctgctc
     1381 tgcctgcggc agaagggctt cccggcccag catgcgtgac gctgcatcca cccgaaaccc
     1441 ggacacgcca agctgcagcc acatgccgac gatcttttgg atctctcgcc gcacggcggg
     1501 gtgctcgata ttgaggtcag gttcaaagct gtagaagtgg tgcaggtacc aagcgccggc
     1561 ctgctcatct cgcgcccaga taccctgctc aacgcctggg aagatcacct cggccgccgt
     1621 ctcgtcgggc ggatcatcgg tccagacgta gaagtcccgg tacggtccgc cctcgcgcga
     1681 ctgctggaac catgggtgct ggtccgacgt gtggtggagg acgagatcga tgatcacctc
     1741 aaggccgcgc gcccgggcgt cgtgcaggaa cgcgacacag tctcccagcg agccatggcg
     1801 ctcgtccacc ccgtagtagt caatgatgtc atagccgttg tcgtggttgg gggtgggaaa
     1861 aaaggggagg agccagaggc aggtgacacc aagagaggcg aggtagtcga gccgt
//
LOCUS       DASYWL010000251         2312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_537782, whole genome shotgun sequence.
ACCESSION   DASYWL010000251 DASYWL010000000
VERSION     DASYWL010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537782"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VGR22_09145"
     CDS             <1..353
                     /locus_tag="VGR22_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128771.1"
                     /translation="DDSTADDADDTTDDDTDDNADDTTDEGTDDADDGTEDDGTEDTD
                     DGTDDDADDDTTESTGTATGTGTSTALPDTGVGAGMAPSSGGLMAAMLSVVGFLFMAA
                     AIIARRRAAAPSVR"
     gene            1139..1516
                     /locus_tag="VGR22_09150"
     CDS             1139..1516
                     /locus_tag="VGR22_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128772.1"
                     /translation="MAERFRRRIAHGQYRELFDVSLARILAQAAERRDLDDEVGAVRF
                     ALARLIAEEPNPTRLALAVARLTHAAVATSRERRECSQGQPDGLTEAMVRILAELDEK
                     EKEAGAKATQAFQPLTYNGRESS"
     gene            complement(1595..1837)
                     /locus_tag="VGR22_09155"
     CDS             complement(1595..1837)
                     /locus_tag="VGR22_09155"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEV2128773.1"
                     /translation="MDYRDIITIESGRRGGKPTIRGMRITVQAALDYLAGGMSEAEIL
                     ADFPELSPEDIRACLALAAAGTDPGRTDEDGGAARH"
     gene            complement(1862..>2312)
                     /locus_tag="VGR22_09160"
     CDS             complement(1862..>2312)
                     /locus_tag="VGR22_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128774.1"
                     /translation="GTPTSVVTRGINNPRSGGWDQTQVMIDYQDASAQVDGGMMMPES
                     YPFSSTFQVLCERGAIEYHFRAGGRSVEMAGDVNELVLYPAEGDPERLSVGQRDPYVA
                     EIAYFTECVTTGQPASRATPEDARLALKVALAARSSLDYGEMTPVPV"
BASE COUNT          408 a          808 c          733 g          363 t
ORIGIN      
        1 ccgatgacag cacggccgat gacgccgacg acaccaccga tgacgacacg gacgataacg
       61 ccgatgacac caccgacgaa ggcacggacg atgccgacga cggcaccgag gacgatggca
      121 ccgaagatac tgacgatggc acggatgacg acgccgacga cgacacgacc gagtcgacgg
      181 gcacggcgac cggtacgggc acgagcacgg cgttgccgga taccggtgtc ggcgcgggaa
      241 tggccccgag cagcggtggt ctaatggccg cgatgctgag cgtggtcggg ttcctcttca
      301 tggccgcggc gatcatcgcc cgccgtcgag cggcggctcc ttccgtgcgc tagcggaatc
      361 gcgaccaaag cggatccggc tgagtgagat ggccgggtta ccgctgaaga agaggccctg
      421 gcatcacgcc ggggcctctt ttggttgctg ccttcgacgc gcttcggatg catgctgttg
      481 gaatagatca tcctggcacc ccacgcggcc gcccccgtga gtccacacca ctccctctcc
      541 ccgccccctc ccctggccgt cttgccgtct tccgtctctc gtgttgccct gtaggtcgtc
      601 gacactgccc cacccaccac ctactatcag caacaagcgg tcaccggcgg cgtgcgggcg
      661 ggcccgggca ggcacgtagc gcgccccggc tggccccggt caccaccgcc accgcttccc
      721 cgttcgccac cctccgcact cggcagaccg gctcgacatc gcttcgccat cggtgaggct
      781 atcggagccg gtcagacccc atggagaccg gcggggcagc ggcgcgtcct cccacccgca
      841 cttggccgtg cgcccgggaa cgtgacgtac cacctgagca cggtcacatc aggcgaaatt
      901 cagagaggcg tcgcctgcta cgtcgttttg tgaagaccat cacatgagag gagcatcaaa
      961 ggtcatcatg gcagaacacc gcctctgcgt cgttccagca tgccgccgtt atgcggccac
     1021 cggatctacc gtctgctggc cccatcgtaa gagtcccact ggtcgtgact gggacaccgc
     1081 catccggcag gtggcgcacg atagcagcga ggccgcttcc gaaggcaacg agtcagcggt
     1141 ggcagagcgc ttccggcgcc ggattgcaca cggccagtat cgtgagctct tcgacgtgtc
     1201 gctcgcgcgc atcctggccc aagccgccga gcgccgcgac ctcgatgatg aggtcggcgc
     1261 cgttcggttc gcgctcgccc gcctcatcgc cgaggaacca aacccgaccc gcctcgcact
     1321 cgccgtagcg cggctcaccc acgccgccgt cgccacgtcg cgagagcgtc gcgagtgttc
     1381 acagggccaa ccggacgggc tgacggaagc gatggtccga atcctcgcgg aactcgacga
     1441 gaaggagaag gaggcagggg ccaaagcaac gcaagcgttc cagccactca cgtacaatgg
     1501 ccgggagtca tcctaatcgg ggatgagtct tccaggccca atgcctagcc ggtcttgaac
     1561 cggcttcgtt ccatgagagc ggagcgcggc accatcagtg ccgtgcggcg ccaccatctt
     1621 catccgtcct acccggatcg gtaccagcgg cggccaaggc caggcaggct cggatgtcct
     1681 ccggggaaag ttccgggaaa tccgcgagga tctccgcttc gctcatgccg ccagcgaggt
     1741 agtccagcgc ggcctgcacg gtgatgcgca tgccgcgaat agtgggcttg ccgccgcgcc
     1801 tgccggactc gatggtgatg atgtcgcggt agtccacggc agaccctctc accgcgcggc
     1861 gctagaccgg aaccggtgtc atttcgccgt aatccaacga cgaccgcgcg gccagtgcga
     1921 ccttgagcgc caggcgcgcg tcctccggcg tggcgcggct cgccggctgg ccagtggtga
     1981 cgcactcggt gaagtaggcg atctccgcga catagggatc gcgctgcccg acgctaagcc
     2041 gttccggatc gccctccgcc gggtacagca cgagctcgtt gacatccccc gccatctcaa
     2101 cgctccgccc gcccgcccgg aagtggtact cgatcgcacc gcgctcacag aggacctgga
     2161 acgtcgagct aaagggatag gactcgggca tcatcatgcc accgtctacc tgggctgagg
     2221 cgtcttggta gtcgatcatc acttgggtct gatcccagcc gccggagcgt gggttgttga
     2281 tgccgcgggt aaccaccgat gtcggcgtgc cg
//
LOCUS       DASYWL010000252         9504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_463382, whole genome shotgun sequence.
ACCESSION   DASYWL010000252 DASYWL010000000
VERSION     DASYWL010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9504
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463382"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /locus_tag="VGR22_09165"
     CDS             complement(<1..1139)
                     /locus_tag="VGR22_09165"
                     /inference="COORDINATES: protein motif:HMM:NF024598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEV2128775.1"
                     /translation="MPRIPDDLTRTHPSRPLPWLPWAASILFLIGIIALVGTLIAAMG
                     GDDDADARLQVLDAQTNGPIANAAVVIDGETYSTDENGWVYLPEGSGDVAIAVAHDGY
                     VGREGTVAITSDQRWSVTLRPDLETQVALEATVETAAEQEQTLETGDASAPEIVVAPS
                     AGSAQQTVAVATPSLPPAPHTELAGRITDAAGEPVQGALLTDGQNVEITGQDGLFTVK
                     AQEFASETLRVSAPGFADTALSFKQAGQPIELQMELQPVKAIYYNPNISSTPEDVDRL
                     IEITNTTEVNAIVVDVKEELIFYDSQVEFFRDVDVVTPVIDVPALLARFDEHNIYTIA
                     RHVVFKDSLVAERRPELAVRHVATGEVWRDMNGVAWVNPMLHDLW"
     gene            complement(1401..2366)
                     /locus_tag="VGR22_09170"
     CDS             complement(1401..2366)
                     /locus_tag="VGR22_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEV2128776.1"
                     /translation="MQPLKIVLCSHEGEQHFHLLDEVEDTTVVNCPPEAPDELLAAAA
                     DMDVFFGKPSVELLRQAPKLKWIQAPSAGVEFVASIPELANADVLLTNTRGAHGPSIG
                     EHAIALLLAMTRYIPDSVRQQDQRIWDRSILYRTCREIGGMTMGLIGFGALGRGVAQR
                     ALGMEMEVIAVDAQAVDGTGLIDEVWPASRLDDLLEQSDVVVVTTPYTELTADLIDAD
                     RLAKMKSDAYLIVVSRGGIVNEEALVDALKAGRLAGAALDVTLPEPPEPDSPLWDCPK
                     LLLTPHTAGASARKEQRVVEIFVDNIRRFQNGEPLVNLVDKTRGY"
     gene            complement(2438..3277)
                     /locus_tag="VGR22_09175"
     CDS             complement(2438..3277)
                     /locus_tag="VGR22_09175"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEV2128777.1"
                     /translation="MRVNHVRRRLEAGEPSIGTWLSLPSPEAAEYVAELGLDWLVVDT
                     EHNAIDIATLARMFMAMRASGVAPMVRIPWNTAENVKRVLDAGAWGIVFPMVNSREEA
                     ELAVEAARYHPAGNRSVGGGRHGMSFGSTSKEYYENANAEVLVVVQIEHIRGVEHADE
                     ILSVPGVDACFIGPNDLAASMGHGLGVSLETDITEVVSAIEAIRDTCRKHGVTPGIHT
                     SGAAAVQRRIGEGFQFCAMASELKYMVGSMASDLQNLDWSPSDRTQQSVGTAAEAGTL
                     VRY"
     gene            3695..5089
                     /locus_tag="VGR22_09180"
     CDS             3695..5089
                     /locus_tag="VGR22_09180"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV2128778.1"
                     /translation="MSKDRSAQLIAEAKAMKYSRRTVMKRASAMGVSASALGGVLSAT
                     GHTVSAAPNRAPAFLQGGSLNFLISTAFVPAAEPFFQQQAQSWGEENGVEVTVDFINW
                     PDLQPRITSAVQAGAGPDIVELWPTIPYLFAENLVEVNDLAAAIDQQHGGFYDWVTNT
                     VAVNGEWRSLPHGTSSIAVAYRKSLFEQAGVEDPENNFPDTWEAFFEVGRRLKQDVGV
                     PLGQSLGQSLGDPPGFTYPYMWSFGSMEVEEDRTTVAFDTELFREGLEMFVQAWTDAF
                     DETGLGWDDSTNNSAYLSGQIAATLNGSSIYLAATDEEGENYNPELAEDTFHAPLPAG
                     PAGQFAELGSRSMGILGYSPNQEAAKAFLEWWFSPEPFQAWLEVYEGYIIPPGTTFVD
                     LPVFTEDPKLAPFLEIVEIGRNKGYAGPANLEAAQAASQYVVVNTFAQAVQTGDVGAA
                     IEQGARHLERIYGR"
     gene            5198..6136
                     /locus_tag="VGR22_09185"
     CDS             5198..6136
                     /locus_tag="VGR22_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV2128779.1"
                     /translation="MSSLTTEKPTRPVIPTVRESFWARLSRRESSLAWLFLLPGLLVL
                     VVFMAYPFAYGIYISMTDAFVGFAEKNFIWLENYRRLLDDPVFRQTVRNTFIYGVGTV
                     PFKLVLGLWLALVLNQSFRFSRMFRAFLLLPWIVPTAISALAFLMLYNGVMSPISWVL
                     EDWGVISGNINFLGDPVNAIISLCVANVWRGVPFFGISILAGLQAVPDDLNEAAALDG
                     ANTWQRFWTVTWPVIRDITLIATLFSIIWTFADFQLVYVLTKGGPANSTHIFGTYSYQ
                     TLGYTDIGTAAAISIYMVPILAVLAILLLRYVRREN"
     gene            6158..7009
                     /locus_tag="VGR22_09190"
     CDS             6158..7009
                     /locus_tag="VGR22_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV2128780.1"
                     /translation="MAMVGANVRRNRWLNLYLPLTFFMVFLLFPFYWMLIVSFKPNSE
                     LLDTNVNPFILLSATLDHYSYLFTDTSFLRYTWNSMVVTIGSTILALSASILIGYALG
                     RFRFKGGNLMGAVIFLAYLVPPTLLFIPLSQVVSNLGLYNNYWSLILTYPTFLIPFAS
                     WLLMGYFRTISIDLEHAALVDGATRLQAMRHIVLPLAMPGILSAGIFCFTLAWNEFLY
                     ALVFMGSGNMKTVPVGVVTDLIRADVYFWGSLMAAGILGSVPVAIAYSFFVDNYISGM
                     TAGATKG"
     gene            complement(7126..8688)
                     /locus_tag="VGR22_09195"
     CDS             complement(7126..8688)
                     /locus_tag="VGR22_09195"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEV2128781.1"
                     /translation="MSTSDDQRSSTPDHQDPGDRVSQIMEQRQLLVASNRGPVTFTQR
                     PDGSFSARQGSGGLVTAVSAVLQGQQAIWIAAAMTEGDRARAAAAEEAGETLITPHGS
                     GSNFRLRFVIPSQDTYNKYYNEISNPLLWFLQHYLWDTPRSPDITHETWSAWREGYVP
                     VNEMFAEEILHASDQLDAEPIIMLQDYHLYLCGKMIRQKCPDAIIQHFVHIPWPDPDY
                     WRLLPTEMRRAICEGLLGCDIVGFQTPDHARSFLNTCVAYVPDVDVSYGTGEITWQGR
                     CIKVAHYPISIDTEAVRRTAHSQEARAYERYLPTYFNEFTILRIDRAEPSKNIVRGFH
                     AFDRFLEKHPEYKNRVSFIAVTQPSRMDVEEYRDYLDDMSATIGRINAKYANVETGWQ
                     PITIIMGESYPRVLAAMRWYDVLLVNSIIDGMNLVAKEGALINERSGVLILSEGAGAA
                     LQLGDHALMVSPADVEGTADAIHNALTMPFSERQRRARALREAVERQDVRLWFQDQLA
                     DIADYVQLQPTA"
     gene            complement(8859..>9504)
                     /locus_tag="VGR22_09200"
     CDS             complement(8859..>9504)
                     /locus_tag="VGR22_09200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEV2128782.1"
                     /translation="WATRGAAVLSVIRGNEVIGIIALEDRVRTEAKVAVEALHRRGIH
                     TIMMTGDARNVAEHVAQQIGIDEVRAEVLPEHKSSEVAQLQRQGRKVVMVGDGVNDAP
                     ALTAADVGLAIGAGTDVAIESADVVLASSDPRAVESVIRLSMASYRKMIQNLWWAAGY
                     NVIAIPLAAGVLAGIGFVMPPAVGAVLMSLSTVIVAINALTLRRLDLTPSAAPA"
BASE COUNT         1612 a         2976 c         3071 g         1845 t
ORIGIN      
        1 ttccacaggt cgtgcagcat cgggttgacc cacgccacgc cgttcatgtc gcgccacact
       61 tcgccggtgg ccacatgccg cacggcgagc tcgggccgtc gttccgcgac caggctgtcc
      121 ttgaagacga catggcgagc gatcgtgtag atgttgtgct catcgaagcg cgcgagcagt
      181 gccggcacgt caatgaccgg agtgaccaca tcgacatcgc ggaagaattc gacttggctg
      241 tcgtagaaga tcaactcctc cttgacatca accacgatgg cgttgacctc cgtggtgttg
      301 gtgatctcga tcagccggtc gacatcttca ggcgtcgagg agatgttcgg gttgtagtag
      361 atcgccttga ccggctgcag ctccatctgg agctcaatgg gttgtccagc ctgcttgaac
      421 gagagtgcgg tgtcggcaaa ccccggcgcg gagacccgga gggtctcgct ggcgaactcc
      481 tgcgccttga cggtgaaaag accgtcctga ccggtgatct cgacgttctg tccatcggtg
      541 agcagcgcgc cctgaaccgg ctccccagcg gcgtcggtga tgcggccagc aagctcggtg
      601 tgaggagccg gtggcaggga cggtgttgcc actgccactg tctgctgcgc gctcccggcg
      661 gatggtgcga ccacgatctc cggtgcgctc gcatccccgg tctcgagcgt ttgctcctgc
      721 tctgcagccg tttcgaccgt cgcctcgagc gccacctggg tctcgaggtc gggccgcagc
      781 gtgaccgacc accgctggtc actggtgatg gcgacggttc cttcccgacc gacgtagcca
      841 tcgtgtgcga cggcgatcgc gacatcgccg gagccttccg gcaggtacac ccagccgttc
      901 tcatcagtgc tgtaggtctc gccgtcgatc acgacggcgg cgttggcgat cgggccgttc
      961 gtctgggcat cgagcacctg caggcgggcg tcggcgtcgt cgtccccgcc catcgcggcg
     1021 atgagggtcc caacgagggc gatgatgccg atcaggaaga ggatgctggc cgcccatggc
     1081 agccacggca gcggtcggga gggatgggta cgcgtgaggt cgtcggggat gcgaggcatg
     1141 cactctctcc ggcggccgcc cgggcggcgt cgagcgaatc gactggattg gaacgtcgga
     1201 acagactgtg gactcgagtg tccagcagtg atcgacagaa gcgtaccacg cggcatcgat
     1261 gtcgcgagtg tcaatcgatg ccggtcagtg tcctcgttag acgacgggat gatgacagat
     1321 cgcagcggga tcaatacgct cacgcacgca gcctgcaaac tgctgcaggg atggcgtgga
     1381 gccggtcctt ctcgctatgg ctagtagccg cgggtcttgt cgacgagatt cacgagcggc
     1441 tccccgttct ggaagcggcg gatattgtcg acgaagatct cgaccacccg ctgctccttg
     1501 cgtgccgagg caccggcggt gtgcggagtc agcagtagct tcgggcagtc ccacagcgga
     1561 ctgtcgggtt ccggcggctc gggcaaggtg acatcgagtg ccgcgccggc gagccgcccg
     1621 gccttgaggg cgtcgacgag ggcctcctcg ttgacgatac cgccacggga cacgacgatg
     1681 aggtacgcgt ccgacttcat cttcgccaac ctgtcggcgt cgatcaggtc ggcggtcagc
     1741 tcggtgtagg gtgtcgtgac aacgaccacg tcgctctgtt caagcagatc atcgagacgg
     1801 ctggctggcc agacttcgtc gatcaggccc gtgccgtcga ctgcttgggc gtcgacggca
     1861 atgacctcca tctccatgcc gagcgcgcgt tgggcgacgc cgcgaccaag cgcgccgaag
     1921 ccgatcaggc ccatagtcat gccgccgatc tcacggcagg tccggtagag gatcgagcgg
     1981 tcccagatgc gctggtcctg ctgtcggaca ctatccggga tgtaccgggt catggcgagc
     2041 agcagcgcga tggcgtgctc cccgatggac gggccgtgcg cgccgcgggt gttggtgagc
     2101 agcacgtcag cgttggcgag ttcgggaata ctggccacga actccacgcc ggcactcggc
     2161 gcctgaatcc acttgagctt cggggcctgc ctgagcagtt ccacagaggg cttgccgaag
     2221 aagacgtcca tgtcggctgc cgcggcaagc agttcgtctg gtgcttccgg cgggcagttg
     2281 acgacagtgg tgtcctcgac ctcgtcgagc agatggaaat gctgctcacc ttcatgggag
     2341 cagaggacga tcttgagcgg ctgcatcgtg atgcgggtct cctgtgtcag atggggcgat
     2401 ggcgggcttt aagcccgccc gcagtatcag ggcagtgcta gtaccgcacg agggtgccgg
     2461 cttcggccgc tgtgccgacc gattgctgcg tccggtcgga cggcgaccag tcgaggttct
     2521 gcaggtcgct cgccatgctg ccgaccatgt acttcagctc gctcgccatc gcacagaact
     2581 ggaatccctc gccgatgcgt cgctgaaccg ccgccgcgcc ggaggtatgg attcctggcg
     2641 tgacaccgtg cttcctgcag gtgtcacgga tcgcctcgat cgcggagacg acttccgtga
     2701 tatcggtctc cagcgagacg ccgagcccat gccccatcga cgccgcgagg tcgttcgggc
     2761 cgatgaagca ggcgtcgact ccgggcacgc tgaggatctc gtcggcatgc tcgacaccac
     2821 gaatgtgctc gatctgcacc accactaata cctcggcgtt ggcgttctcg tagtactcct
     2881 tcgaggtcga tccgaagctc atgccgtgcc ggccgccacc gaccgagcgg ttgccagcgg
     2941 ggtggtagcg tgctgcctcc acggccagct cggcctcctc gcggctgttg accatgggga
     3001 agacgatgcc ccacgctccg gcatcgagca cgcgcttgac gttctcggcc gtgttccacg
     3061 ggatgcggac catcggcgcg acgccggagg cgcgcatcgc catgaacatg cgtgcgaggg
     3121 tcgcgatgtc gatcgcattg tgctcggtgt cgacgacgag ccaatcgaga ccgagctcgg
     3181 cgacgtactc cgcggcttcc ggcgagggca gcgagagcca agtgccgatg gatggctctc
     3241 cagcctcgag acgtcggcga acatggttga cgcgcatgat ttcctccgaa cggtgacgac
     3301 aagtattcgc tgttgtggca gcctagcaga cccgccgata ccacggaaga gggtgcagcg
     3361 ccatgatgtt gggcttcgac atcgtgaaag attgcacgat attggcgatt cgcctcttcc
     3421 ctgtcacaat agggccacgt ttccttcaga cgaagaaaca caagggttga acgcccgagg
     3481 cggcccccac ccgggggaaa cccggaatcg ggcatgaccg gtgcagtggt gtttcgcgtc
     3541 gaggggaacc ctgatatcat cagtgtgcgt acgaaattga agagcgacga aggagggatg
     3601 cgcgaagacc gccgtttgaa gggatcggat atcaccgttc gatgcagggg gcgagcgaat
     3661 cgacgccgca ccgcatgagc aggagaagga tgctatgtcc aaggaccgtt ccgcacagct
     3721 gatcgccgaa gcgaaggcga tgaagtactc gcgcagaacc gtgatgaagc gggcatcggc
     3781 gatgggggtc tctgccagcg cgctgggcgg cgtgttgagt gccacgggcc atactgtttc
     3841 cgccgcaccg aaccgcgctc cggccttcct gcagggcggc agcctcaact tcctgatcag
     3901 cacggccttc gtgcccgccg ccgagccctt ctttcagcag caggcgcaat cctggggcga
     3961 ggagaacggc gtcgaggtca cggtcgactt tatcaactgg cccgacctgc agccgcgcat
     4021 cacttctgcc gtgcaggcgg gcgccggacc cgacatcgtc gagttgtggc cgaccattcc
     4081 ctacctcttc gcggagaatc tggtcgaggt gaacgatctt gcagcggcga tcgaccagca
     4141 gcacggcggg ttctatgact gggtgaccaa cacggtcgcc gtcaatgggg agtggcggtc
     4201 acttccgcac ggcacctcgt cgatcgccgt cgcctatcgc aagagcctct ttgagcaggc
     4261 gggcgtcgag gatccggaga acaacttccc cgatacgtgg gaggcgttct tcgaggtcgg
     4321 caggagactc aagcaggacg tcggcgttcc gctcggccag tcactcggcc agagcctcgg
     4381 tgacccgcca ggtttcacct acccgtacat gtggtcgttc ggctccatgg aagtcgagga
     4441 ggaccgcacc accgttgcct ttgacacaga gctgttccgc gagggactgg agatgttcgt
     4501 ccaggcgtgg acggacgcct tcgacgagac cggtcttggc tgggacgact cgaccaacaa
     4561 cagcgcctac ctctccggcc agatcgcggc gacgctcaac ggctccagca tctaccttgc
     4621 ggcaaccgat gaggagggcg agaactacaa tccggagctt gccgaggaca ccttccacgc
     4681 gccgctgccc gccgggcccg cggggcaatt cgccgagctg ggcagccgct cgatgggtat
     4741 ccttggctac tcgccaaatc aggaagccgc gaaggcgttc ctggagtggt ggttctcgcc
     4801 agagccgttc caggcctggc tggaggtata cgagggttac atcatcccgc cagggacgac
     4861 cttcgtcgac ctcccggtct tcaccgagga tccgaagctg gctccgttcc tcgagattgt
     4921 ggaaatcgga cgcaacaagg gttacgccgg cccggcgaac ctcgaggcgg cacaggcggc
     4981 gtcgcagtat gtggtcgtca atacctttgc ccaggcagta cagacgggcg atgttggagc
     5041 ggcgatcgag cagggcgccc ggcacctgga gcgcatctac ggtcggtagc gacgcggctg
     5101 tcgtcgatcc acgacatagc acgagtgagc gagccacggc tcgctcactc gtgtggtgaa
     5161 taccggttcc agtctctccc gcagtcagga agcagccatg agttcgttga ccaccgaaaa
     5221 acccacgcga ccggtcattc ccacggttcg cgagagcttc tgggcacgcc tgtcgcgccg
     5281 ggaatcgtcc ctcgcgtggc tgtttctcct tcccgggctg ctcgtcctgg tcgtgtttat
     5341 ggcctacccg ttcgcctacg gcatctacat ctcgatgacg gatgccttcg tcgggttcgc
     5401 tgagaagaac tttatctggc tcgagaacta tcggcggctt ctcgatgacc cagtcttccg
     5461 acagaccgtt cgcaacacgt tcatttacgg ggtgggcacg gttccgttca agctggtgct
     5521 gggattgtgg ctggcactgg ttttgaacca gtcgttcagg tttagccgga tgttccgagc
     5581 gttcctcctg ctgccgtgga tcgtgccgac tgcgatcagc gcactggcgt tcctcatgct
     5641 ctacaacggc gtgatgagcc cgatctcatg ggtactggag gattggggcg tcattagcgg
     5701 caacatcaac ttcctcggtg atcccgtgaa tgccatcatc tcgctgtgtg tggccaatgt
     5761 ctggcgtggc gtgccgttct tcggtatctc gatcctggct gggttacagg ccgtgccgga
     5821 tgacctgaac gaggcggcgg cgctggacgg tgcgaacacg tggcagcgtt tctggaccgt
     5881 gacgtggccg gtgatccggg atatcacgct gatcgcgacg ctgttctcga tcatctggac
     5941 gttcgccgac ttccagctcg tctacgtgct taccaaaggc gggcccgcaa actcgacgca
     6001 tatcttcggt acgtactcct accagacgct cggctatacc gacatcggca ccgcagccgc
     6061 catctccatc tacatggttc caattctggc cgtgctcgcg attctcctgc tgcgctacgt
     6121 gcgccgggaa aactagggca gaagggagac aacgctcatg gcaatggttg gcgcgaacgt
     6181 gcggcgaaac cggtggctca atctctatct gccgctcacg ttcttcatgg tgttcctgtt
     6241 gttcccgttc tactggatgc tcatcgtgtc cttcaagccg aattccgaac tgctggacac
     6301 gaacgtcaat ccgttcattc tcttgagcgc gacgctggac cactattcgt acctgttcac
     6361 ggacaccagt tttctccgct acacctggaa ctcaatggtg gtaaccatcg ggtcgacaat
     6421 ccttgcgctg agtgccagca tcctgattgg ctatgcattg ggccggttca ggttcaaggg
     6481 tggcaacctg atgggcgccg tgatcttcct ggcctatctt gtgccgccga cgctcttgtt
     6541 cattccgctc tcacaggtcg tctcgaatct gggtctctac aacaactact ggtcgcttat
     6601 cctgacctat cccacgttcc tgatcccatt cgccagctgg ctcctgatgg gctacttcag
     6661 gacgatctcg attgacctcg agcacgcggc gctcgtggac ggggcgactc gactgcaggc
     6721 aatgcggcat atcgtgctgc cgctggcgat gccgggcatc ctctcggcag gtatcttctg
     6781 tttcacgctg gcgtggaacg agttcctcta tgcgctcgtg ttcatgggct ccggaaacat
     6841 gaagacggtg ccggtcggtg tggtgaccga cctgatccgc gcggacgtct acttctgggg
     6901 ctccttgatg gcggcgggca tcctggggtc ggtgccggtg gcgatcgcgt attccttctt
     6961 cgtggacaac tacatctcgg gcatgaccgc gggcgccacc aaggggtagt gactcaagac
     7021 agttggagag cgcaaaatgc ggccccgctc gccgggcggg gccgcttcgg tcatgcttga
     7081 tgcggtcgtc ggtcgcacta ggcgacgttt ggggatgatg acccgtcagg cggtcggctg
     7141 cagctggacg tagtcggcga tgtcggcgag ctgatcctgg aaccacagcc gcacatcctg
     7201 ccgctcgacc gcctcccgca atgctcgcgc gcggcgctga cgctcgctga acggcatggt
     7261 cagcgcgttg tggatggcat cggccgtgcc ctcgacgtcg gccggggaga ccatcagtgc
     7321 atggtcgccc agctgcaggg cagctccggc tccctcggac aggatcagga caccgctccg
     7381 ctcgttgatg agcgcgccct ccttggcgac gaggttcata ccgtcgatga tcgagttgac
     7441 cagcaggacg tcgtaccagc gcatcgccgc cagcacgcgc gggtaactct cccccatgat
     7501 gatcgtgatc ggctgccagc cggtctcgac gttggcgtac ttcgcgttga tccgaccgat
     7561 ggtcgcgctc atgtcgtcga gatagtcccg gtactcctcg acgtccatac gtgacggctg
     7621 ggtgacggcg atgaagctga cccggttctt gtactccggg tgcttttcga ggaaacggtc
     7681 gaaggcgtgg aagccgcgga cgatgttctt gctcggctcc gcccgatcga tgcgcaggat
     7741 ggtgaactcg ttgaaatatg tcggcaggta tcgctcgtag gcacgcgcct cctgactgtg
     7801 cgcggtgcgg cggacggcct cggtatcgat cgagatgggg tagtgtgcta ccttgatgca
     7861 ccggccctgc cacgtaatct cgcctgtccc gtagctcaca tcgacatccg gcacatacgc
     7921 cacgcaggta ttcaggaagc tgcgggcatg gtccggtgtc tggaagccaa cgatgtcgca
     7981 gccgagcaac ccctcgcaga tggcacgtcg catctctgtc ggcaagaggc gccagtagtc
     8041 ggggtcgggc cacgggatgt gaacgaagtg ctggataatc gcgtccggac acttctgccg
     8101 gatcatcttg ccgcacaggt agaggtggta gtcctgcagc atgatgatcg gctctgcgtc
     8161 gagctggtcg ctggcgtgca ggatctcctc ggcgaacatc tcgttgaccg gcacgtaccc
     8221 ctcacgccat gccgaccaag tctcatgggt gatgtcgggg gagcgcgggg tatcccacag
     8281 gtagtgctgc aggaaccaca gcagcgggtt ggagatctcg ttgtagtact tgttgtaggt
     8341 gtcctgcgac gggatcacga accggagcct gaaattggag ccggagccgt gcggggtgat
     8401 gagcgtctcg cctgcctcct cggcggcggc ggcgcgggcg cggtcaccct cggtcatggc
     8461 ggcggcgatc cagatcgcct gctggccctg gaggacggcg ctgacggccg tgacgagacc
     8521 gccgctgccc tgccgcgcag agaagctgcc atccggacgc tgggtgaagg tgaccggacc
     8581 gcggttggag gcgaccagca gctgtcgctg ctccatgatc tggctcacgc gatcccctgg
     8641 gtcctggtgg tccggagtgc tgctgcgctg atcgtcgctc gtgctcatgc tggtgacctc
     8701 ctcatgcagg cgtgcagatc cgagtcggcc cagacccatc gccggtcggt acgacgcccg
     8761 gcacggttcc ccaccgaatg tcgcactgct gtggcctgat tatccccgat tacatcctgc
     8821 gtggcacgca cgagcgaacc ggctgcttcg cgggctactc atgccggtgc cgccgatggc
     8881 gtgagatcga gcctgcgcag cgtcagcgcg ttgatcgcga cgatgaccgt cgacaggctc
     8941 atcagcaccg cgccgaccgc tggcggcatc acgaagccga ttccggcgag cacgcccgcg
     9001 gccagcggga tggcgatgac gttgtagccg gccgcccacc agaggttctg gatcatcttg
     9061 cggtacgacg ccatcgagag ccggatgacg ctttccactg cacgtggatc gctcgatgcg
     9121 agcaccacgt cggccgactc gatcgccaca tcggtgcctg cgccaatcgc gagccccacg
     9181 tcggcggcgg tcagcgccgg agcgtcgtta acgccgtcac ccaccatcac cactttgcgg
     9241 ccctgccgct ggagctgcgc gacctcgctg gacttgtgtt ccggcagcac ctcggcgcgt
     9301 acctcgtcga tgccgatctg ctgcgcgacg tgctcagcca cattgcgggc gtcaccggtc
     9361 atcatgatgg tgtgaatgcc ccgccgatgg agcgcctcca cggcaacctt cgcctcggtc
     9421 cgaacccgat cctccagcgc gatgatcccg atcacctcgt tgccgcggat aaccgagagg
     9481 accgctgccc cccgagtggc ccac
//
LOCUS       DASYWL010000253         5776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_162800, whole genome shotgun sequence.
ACCESSION   DASYWL010000253 DASYWL010000000
VERSION     DASYWL010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5776
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162800"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="VGR22_09205"
     CDS             <1..1128
                     /locus_tag="VGR22_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2128783.1"
                     /translation="LVAIMPQHLWADVPPGDWGTDPGSTGQDPSRVVGSGPFRFVEWV
                     QEDHVTLEKYADYWDTNFVPNIDTFTYRVIPEQSSSTQALLTGEIDWTELSEAEGRQL
                     AEEPELNVHAFDTTGVNWYSANQDPSRTELFTDVRVRQALMLALDRQLLGDQVYEGYA
                     IPANGTQPVLSVAYRPEAITVDLSYDPERAMSLLEEAGWVDEDGDGIREKDGVKFSFE
                     CLFSEGVATYEQQLPYMQQAWAEIGIEMLPTAVPFTALLDATDTGEYQMAVYGFSWSI
                     DGGQLPMFGCEYLPPQGFNSMRYCNEEYDQLALAAEKELDPERRIEMLVEASNIVWEE
                     QAAGYIVFRQQILGSRKTLHNFFPNGYSEMWGVSFLWTEVQ"
     gene            1308..2279
                     /locus_tag="VGR22_09210"
     CDS             1308..2279
                     /locus_tag="VGR22_09210"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2128784.1"
                     /translation="MSRYLLRRLIGIIPLLLGISFVVYALLNLVPGSPTDQFEFNPNI
                     RPEDRERIEENLGLNAPWYARYFTWLANVLQGDLGNSFTNFAPVENAITAALPNTLIL
                     SVTALIFALVLSVPIGIISAVRLNSFFDRFVTMVSTAFFSIPSIWLGLLLIILFSVKF
                     QEWGMPFSLPTGGVRDLRGESGFWDRVEHLILPAFTLGVVQLAGWTRYVRSSMLEVIR
                     QDYIRTAEAKGLRQRAVLMGHAFRNAMLPLVTLVGLTIPDLFGGALIVENVFAYPGIG
                     RLTVESLNQNDYSVAMAAIMMLAFLTVLGNLIADVMYGVLDPRVRFD"
     gene            2311..3261
                     /locus_tag="VGR22_09215"
     CDS             2311..3261
                     /locus_tag="VGR22_09215"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2128785.1"
                     /translation="MAHQSETSSAATARLADEKSKLTALDRSHLRSTPGFYNRAWGRF
                     RKDKVAMVALVIAIAILIFSFGAPVVSELTGYGYETMDLRKKLLAPGEQGHLLGTDSN
                     GRDILTRLAYGGRVSMMVSVLAVISALVIGGAVGSIAGYYGGFIDSVLMRLVDVVISI
                     PGITILLLLSVWFRPGPVGLAFVIAGLAWTGISRLVRGEVLTLKNRDYIDAARVLGAS
                     NSRIIVRHIFPNVLSIIIVWMSLALPALILYEATLSYLGFGVRIPVPSWGNMLDEAKE
                     FYRSSWTYVFIPGFAIYLAALAFNLVGDGLRDALDPRLNE"
     gene            3358..3741
                     /locus_tag="VGR22_09220"
     CDS             3358..3741
                     /locus_tag="VGR22_09220"
                     /inference="COORDINATES: protein motif:HMM:NF024645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEV2128786.1"
                     /translation="MECPQCGHRNGPGDRFCSNCGTRLDGGASIPRQYEPPERSDGTP
                     PPPPPTPREDIPEDPADPEWRMSPVPDAEPPKRRTWLWVLAGLIIFCVLLFCGFSLFV
                     TSTDTGREWIDDLATRAAEVATPVD"
     gene            3769..4589
                     /locus_tag="VGR22_09225"
                     /pseudo
     CDS             3769..4589
                     /locus_tag="VGR22_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            4686..5531
                     /locus_tag="VGR22_09230"
                     /pseudo
     CDS             4686..5531
                     /locus_tag="VGR22_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012890904.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          959 a         1961 c         1820 g         1036 t
ORIGIN      
        1 ctcgtggcga tcatgccgca gcacctctgg gctgacgtac cgcccggtga ctggggcacc
       61 gatcccggct cgaccggtca ggatcccagc cgtgtggtcg gctcagggcc gttccgcttc
      121 gtcgagtggg tgcaagagga ccacgtcacc ctcgagaagt acgcggacta ctgggacacc
      181 aacttcgtcc cgaacatcga caccttcacc taccgcgtga tcccggagca gtcctcctcg
      241 acccaagcgc tgctcaccgg cgagatcgac tggactgagc tcagcgaggc cgaaggacgg
      301 caactcgccg aggagccgga gctcaacgtt cacgccttcg acaccaccgg tgtcaactgg
      361 tactcggcaa accaggatcc atcaaggacc gagctcttta cggacgtgcg cgtgcgccag
      421 gcgttgatgc tcgccctcga tcgccagctg ctgggcgacc aggtctatga gggctacgcg
      481 atcccggcga acggcaccca gccggtgctc tcggttgcgt accgacccga ggccatcacc
      541 gttgacctca gctacgaccc cgagcgggcc atgtcgctgc tggaggaggc cggctgggtc
      601 gacgaggacg gcgacggcat ccgcgagaag gacggcgtga agttcagctt cgagtgcctc
      661 ttctcagagg gtgtcgccac ctacgagcag cagctgccgt acatgcagca ggcatgggcg
      721 gagatcggca tcgagatgct gccgaccgcc gtgccgttca ctgcgctgct cgacgccacc
      781 gacaccggtg agtaccagat ggccgtctac ggcttcagct ggtcgatcga cggtggccag
      841 ctgccgatgt tcggctgtga gtacctgccg ccgcagggct tcaactcgat gcggtactgc
      901 aacgaggagt acgatcagct ggccctggcg gccgagaagg agctcgaccc cgagcggcgc
      961 atcgaaatgc tggtcgaggc gtccaacatc gtctgggagg agcaggcggc cggctacatc
     1021 gtcttccgcc agcaaatact cggcagccgc aagacgctgc acaacttctt cccgaacggg
     1081 tacagcgaga tgtggggcgt ttcgttcttg tggacggagg tccagtagca atcctcaatc
     1141 caagcctgca tcgcagcatt tggaatggag ggggccgggc ggccccctcc tcgcccgtca
     1201 cctccacagg tgcctcggct gccgatgtcc gtgaacaacg ccgtatgctt cgcgtggctg
     1261 ttgcacttcg agctcgcttt tgacagttca ttgtcaggga ccgcgcaatg tccaggtatc
     1321 tcctccgcag gctgatcgga ataattcccc tcctcctggg gatttcgttt gtggtgtacg
     1381 ccctgctcaa tctcgtgccg ggtagcccga ccgatcagtt cgaattcaac cccaatatca
     1441 gaccggaaga ccgggaacgt atcgaggaga atctcgggct caacgcgccg tggtacgcgc
     1501 gctacttcac atggctcgcg aatgtgctcc agggcgacct cggcaacagc ttcaccaact
     1561 tcgcccccgt cgagaacgcc atcaccgccg ccctgccgaa cacgctgatc ctcagcgtga
     1621 cagcgctgat tttcgcgctg gtcctgtccg tgccgatcgg catcatctcg gcggtcaggc
     1681 tcaacagctt cttcgaccgg ttcgtgacga tggtcagcac ggcgttcttc tcaatcccat
     1741 cgatctggct cgggctcctg ctgatcatcc tcttctccgt caagttccag gagtggggta
     1801 tgcccttctc gctcccgacc ggcggggtcc gagacctgcg tggcgagagc gggttctggg
     1861 accgcgtcga gcacctgatt ctgccggcct tcaccctcgg agtcgtccag ctcgccggct
     1921 ggacccggta cgtgcgatca tccatgctcg aggtcattcg tcaggactac atccgcaccg
     1981 ccgaggccaa gggcctccgc cagcgcgccg tgctgatggg ccacgccttc cggaacgcca
     2041 tgctgccgct ggtgaccctc gtcggcctca ccattcccga cctgttcggg ggtgccctga
     2101 tcgtcgagaa cgtcttcgcc taccccggca tcggtcgcct cacggtcgag tcactcaacc
     2161 agaatgacta ttccgtggcc atggcggcca tcatgatgct cgccttcctg acggtgctcg
     2221 gcaatctcat tgcggatgtc atgtacggtg tgctggaccc gcgcgtccga ttcgattgag
     2281 cccgtcagca tgtcgaaagg gagccgctag atggcacacc agtccgaaac ctccagtgcc
     2341 gccaccgcca gactggcaga cgagaagtcg aagttgaccg cgctcgaccg cagccatctc
     2401 aggtcgacgc cggggttcta caaccgagcc tggggccgct tccggaagga caaggtggcc
     2461 atggttgcgc tcgtcatcgc catcgccatc ctgatcttct cgttcggcgc cccggtcgtc
     2521 tccgagctca cggggtacgg ctacgagacc atggatcttc gcaagaagct cctcgccccg
     2581 ggcgagcagg gtcacttgct gggcaccgat tccaacgggc gcgacatcct caccaggctc
     2641 gcctacggcg gtcgcgtgtc gatgatggtc tcggtgctgg ccgtcatatc cgcgctggtc
     2701 atcggcggcg cggtcggctc catcgccggg tactacggcg ggttcattga ctcggtgctc
     2761 atgcgcctcg tcgatgtcgt catttccatc ccgggcatca ccatcctgct gctgctcagc
     2821 gtctggttcc ggcccggccc cgtcggcctc gccttcgtca tcgccgggct ggcgtggacg
     2881 ggcatctcgc gcctcgtccg gggcgaggtg ttgacgctca agaaccggga ctacatcgac
     2941 gctgcccgcg tgctcggcgc atccaactcg cgcatcatcg tccggcacat cttcccgaac
     3001 gtcctctcaa tcatcatcgt ctggatgtcg ctggcgctcc cggcgctcat cctctacgag
     3061 gcgaccctct cctacctggg gttcggcgtg cgcatcccgg taccgtcctg gggcaatatg
     3121 ctcgacgagg cgaaggagtt ctaccggtcg tcctggacgt acgtgttcat ccccggcttt
     3181 gcaatttacc tcgccgcgct ggccttcaac ctcgtcggcg acggcctgcg agatgcgcta
     3241 gacccgcgcc tcaacgaata ggattccaac ctcgtatcga aagacgatcg acgcgacggc
     3301 ggaggctgcc acacacgctc cgccgtcgct gttcgcaggg aacagggagc gcgcctcatg
     3361 gaatgtcctc aatgtggcca tcggaacggt ccgggagacc ggttctgcag caattgcggc
     3421 acccggctgg acggcggcgc gtccatcccc aggcagtacg agccacccga gcgaagcgac
     3481 ggcacgccac ctcccccgcc ccccacaccg cgcgaggaca tcccggagga cccggccgac
     3541 cccgagtggc gcatgtcgcc ggtgcccgac gcggagccgc cgaagcgccg gacctggctc
     3601 tgggtgctgg ccggcctcat catcttctgc gtcctgctct tttgcgggtt cagcctcttc
     3661 gtcacctcca cggacaccgg gcgggagtgg atagacgacc tcgctacacg tgccgcggag
     3721 gtcgcaacac ccgtagacta agagtgcctt tcaaaaccgg ggaacgccat ggtagacctg
     3781 tggcatgagc aaaccgctct ccccgacgat ctgtgggcca tcgtcgagcc gctactcccg
     3841 cccgagccac ccaagcccaa gggcggccga ccccgtgttc cccatcgtgc agccatgact
     3901 ggcatcctct tcgtcgtacg ctccggcatc ccttgggagc tgctgcccca ggaaatgggg
     3961 tgtggctcgg gcgtgacctg ctggcggcgc ttgcggggct ggccggtggc cggcgtctgg
     4021 gatcgcgtgc accagacctt gctggaccgg ctcggcacgg cgaaccgcat cgactgggag
     4081 cgggcgagcc tggacagcgc cagcatcccg gccaaagggg ggggcgaggc ggtgggaccg
     4141 aacccaaccg atcgcggcaa accgggcacc aagcgccacc tgatcgttga gcgccagggc
     4201 attccgctgg ccgccatgct caccggggcc aaccgccatg accgccatgg tcttcgagga
     4261 cctgctcgat gccgtgccgc ccctgtgccg tccttcgggg cagcggcgca cccggcccgc
     4321 caagctgcat gccgacacgg acggatgaca tcccgcgctg tcggcaggcg ctgtcgcgtc
     4381 gccacattcg ggtccggatc gcacgcaggg gtatcgattc cagcgagaag ctgggtcggc
     4441 atcgctgggt ggtggagcgc accctggcgt ggctcaatcg ctattgccgg ctggcgatcc
     4501 ggtacgagcg gcgcaccgat atccaccagg cgttcctcac gttgggctgt gcgctcatct
     4561 gcttcaacca cctctcggag gagttttgaa agccactcta agagcgaccg gcactaggtg
     4621 acagacaggc acggcaccga gcatcatggc ggtggttgcc gtcgctgtga tcctcggagg
     4681 ggaccgtgcc tgccgtgcca gtttcgctga tcgagcccat ctgggcccag ttcgccgtgc
     4741 tcttgcccga ccgtccggcg gtggacccga cgcatcccct cggtcgccac cgacgccgag
     4801 tgccggaccg cgtggtgttt gagcacgtcg ttggcgccat ggtgcagggc tccggctacg
     4861 agcgaatcgc ctcaccggac tgttcggatc ggacgattcg acgccggata cacgaatggg
     4921 ccgcggcggg gctgacccag cagttccacg cgctggtgct ggcgcaatat gaccggatga
     4981 tcgggcttga gcttgaggat gtggcgattg acggctgcat cacgaaagcg ccgggtgggg
     5041 gcgagatggc gggccgcagc cccgtggatc gcggcaaaca ggggctgaaa cgcgcgacgg
     5101 ccgtggatgc caccggggtg ccgctccaca tggtggccgc cggggccaac cgccatgacg
     5161 ggccgttgct cggtcccacg ctggcggggc tggagccgct cgggccgctg ccggagacca
     5221 ccgtgcatct ggaccgcggc tacgacagcg gcccgagccg agccctgctg gacgcgttgg
     5281 gcttcactgg cgtgattgcc cgcaagggcg tgccggcacc gctgcaagtc ggtaaacgat
     5341 gggtagtgga gcgcacccaa tcgtgaatga acggctacgg caagctgcgg cgctgcaccg
     5401 agaaaaccaa gaaggtggtg gacttctacc tatatttggc ggcggggtcc gtggtcacgc
     5461 gctgcctgct ccagcgcgcc cgcacgcgct accgctggcc cggccgaccc accacgcggc
     5521 ttctgacgtg atgcctattg ccggtcgctc taagcgcctg cgccgatagg gagagcgctg
     5581 tagtggtgtc gtccgcaaca ctcccatcac cactggctcc aggctgtcag ccatacaaac
     5641 cgccagcacc aattggcgca agcttcaagg tgaatgtaga gaaacccgat cgggtccgtg
     5701 tgaatccgta ggggacgact tggatgcgct tgccccgtcg tcatttccaa cgtcgcgcgt
     5761 atccaatgtc ctcccg
//
LOCUS       DASYWL010000254         3265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_575564, whole genome shotgun sequence.
ACCESSION   DASYWL010000254 DASYWL010000000
VERSION     DASYWL010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575564"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1592)
                     /gene="topA"
                     /locus_tag="VGR22_09235"
     CDS             complement(<1..1592)
                     /gene="topA"
                     /locus_tag="VGR22_09235"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEV2128787.1"
                     /translation="MATTKTTRAGSANGTKRKTTRKKPTNSTPRGKLVIVESPAKAKT
                     IEKYLGRGYKVKASMGHIRDLPKSKMGVDVDHDFEPQYLIPRDKSKVVKELRESVQSA
                     KEIYLATDPDREGEAIAWHLIYATEAHDKPLRRVVFHEITPEAVSAAMEHPRDIDMDL
                     VEAQQARRILDRLVGYGISPLLWKKVKRGLSAGRVQTAALRIVVEREREIDAFVSEEY
                     WSLDADLAHHTGQKPTNADRFRASLQKINGKKAELKAEADATGVVHAVEHADWKVHEV
                     VRRASQRRPQAPFTTSTLQQEAARKLRMNVRRTMSLAQELYEGVNLGPDSTQGLITYM
                     RTDSTNIALSAQQAARAVISTRYGVEYVPAKTPVYSKRSKSAQEAHEAIRPTAPQRDP
                     ESVKQYLSRPQFRLYQLIWQRFIASQMAPAKLDNTRIDVAAIPQGTSADGKTAPYIFR
                     ATGSIVTFPGWMKVYQMGRDDGEKDELDKAALPEVQQGDPIDLEKLIPEQHFTQPPPR
                     YSEATLVKALEEQGIGRPSTYAP"
     gene            complement(2134..>3265)
                     /locus_tag="VGR22_09240"
     CDS             complement(2134..>3265)
                     /locus_tag="VGR22_09240"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV2128788.1"
                     /translation="GKTEREARRKAKQTLRDYEDGLSRRDERLTVRDYLTDWLETTAA
                     NRVRPSTLKNYRFHINNHILPAIGGIQLRELTATDVNRMLAGIVNKGVSPATANLIRA
                     TLVNALSSAVKAQMVRSNAAALSDARREKRTRIVPLTPEQARRLIEATKDDRMGPLIA
                     VAIATGLRQGELLALRWQDVDLERRVLDVHRTLTWQKADEGSHRKRTPAFADPKTAHS
                     RRRVPLTQLAVEAFHRQREVVREMEQAATVASWRPIPGEDLVFVTTVGTPLDGTEVTH
                     RLHRLLEGAGLPQKRFHDLRHSTASFLLAEGADIFTVKEILGHSGISMTADIYGHLTE
                     KLAEDAAARLDRVFADAKTGRSAPKPTPDAIDSDDTETGGDR"
BASE COUNT          506 a         1056 c         1021 g          682 t
ORIGIN      
        1 ggcgcgtagg tgctcggccg cccgatcccc tgctcctcca gcgccttcac gagcgtcgcc
       61 tcactgtagc gcggcggcgg ctgggtgaag tgctgctccg gaatcagctt ctcgaggtcg
      121 atcggatcac cctgctgcac ctcgggaagc gcggccttgt ccagctcgtc tttttcgccg
      181 tcgtcacggc ccatctggta gaccttcatc cagccgggga aggtcacgat cgagccggtc
      241 gcacgaaaga tgtacggcgc ggtcttgccg tcagcagagg taccctgcgg gattgcggcg
      301 acatcgatac gggtgttgtc cagcttcgcc ggagccatct gactcgcgat gaagcgctgc
      361 cagatgagct ggtacagcct gaactggggc ctggagaggt actgcttgac gctctcgggg
      421 tcgcgctgcg gcgcggtcgg tcggatcgcc tcgtgggcct cctgtgccga cttcgagcgc
      481 ttgctgtaga ccggcgtctt cgccggcacg tactccacgc catatctggt actgataacg
      541 gctcgggcag cctgttgtgc agacaacgcg atgttcgtcg aatcggtccg catgtaggtg
      601 atcaggccct gcgtgctatc cgggcccaga ttgacgccct cgtagagctc ctgcgcgagc
      661 gacatcgtgc gccgcacatt catccggagc tttcgggcgg cctcctgctg cagcgtgctc
      721 gtcgtgaagg gcgcctgcgg tcgtcgctgg ctggcccgcc ggaccacctc gtgcaccttc
      781 cagtcggcat gctcgacagc atgcacgacg ccggtggcgt ccgcttcggc cttgagctcg
      841 gccttcttgc cgttgatctt ctggagggac gcgcggaagc gatccgcatt ggtcggcttc
      901 tggcccgtgt ggtgcgcaag gtctgcgtcg agcgaccagt actcctcaga gacgaacgca
      961 tcgatctccc gctcgcgctc gacgacgatg cggagcgccg ctgtctgcac ccggccggcg
     1021 gagaggcctc gcttgacctt cttccagagg agcgggctga tgccgtagcc gacaagccgg
     1081 tcaaggatgc gacgggcctg ctgggcctca acgaggtcca tgtcgatgtc acgcgggtgc
     1141 tccatcgccg cgctcaccgc ctccggcgtg atctcgtgga acaccacacg gcgcagcggc
     1201 ttgtcatggg cctcggtggc gtagatgagg tgccacgcga tcgcctcgcc ctcacggtcc
     1261 gggtcggtcg cgagatagat ctccttcgcg ctctggacgc tctcgcgcag ctccttgacg
     1321 accttcgact tgtccctcgg gatgaggtac tgcggttcga agtcgtgatc gacatccacg
     1381 cccatcttgc tcttgggcag gtcgcggatg tggcccatcg aggccttcac cttgtaccca
     1441 cggccgaggt acttctcgat cgtcttcgcc ttcgccggcg actcgacgat caccagctta
     1501 ccgcgaggtg tcgagttcgt gggtttcttg cgggtggtct tgcgcttggt gccgttcgcg
     1561 ctgccggcac gcgtcgtttt cgtggttgcc atgggagttc acctggtctg gttccgcttc
     1621 ggaggatgca gattcgtcaa catttcgatg tggcactcgc aggccgggtg ccaaccggcg
     1681 ctgttcttcc tatattcatc gtcgcgccag ctgcacaatg gtcgacttcg acccgcgaca
     1741 cacccctgtt ttcagcgtac cggggccgtc aaccacgttg ccgtgcgttg cgacgaatat
     1801 tgacagtagg gattcggcac cgagattgtc aagggctagc gtcacactca catctgctcg
     1861 tcgacggcgc acccggtgct cgtgtgtccg ctatcgtcac gatacatctg cgcgcccccg
     1921 ctaatatgga tcggacggct gatggcacgg tatgcacaca gcgatgatcg gtggagcatc
     1981 gcgaaggacg aaaccgacgg gtcacaccac gcgacttagc cggaccgggc gtagtattgc
     2041 gcaccgacgt tacgaacata cgattgccag ctggtaaaac cgattcaagg ccgatcagcg
     2101 gtgtctgaaa tatcgccgaa atcaaagggc acgtcaccga tcaccacccg tctctgtatc
     2161 gtctgagtca atcgcgtctg gcgtcggttt tggcgcactt cgccccgttt tggcgtcagc
     2221 gaacacccgg tccaggcgcg ctgcagcgtc ctcggcgagc ttctccgtca agtggccata
     2281 gatgtcagcc gtcatggaga tcccgctgtg cccgaggatt tctttcaccg tgaagatgtc
     2341 tgcgccctcg gcaagcagga acgacgccgt cgagtgccgg aggtcatgga accgcttctg
     2401 aggcaagccg gcaccctcta ggagacggtg caggcggtgg gtgacttcgg tcccatcaag
     2461 cggcgtccct accgtcgtca caaacaccag gtcctcgccg ggaatcggac gccagctggc
     2521 aacggtcgct gcctgctcca tctcgcgcac gacctcgcgc tgccggtgga acgcctcgac
     2581 ggcgagctgc gtcagcggta ctcgccggcg cgagtgggcg gtcttcggat cggcaaacgc
     2641 tggcgttcgt ttccgatggc tcccctcgtc ggccttctgc catgtcagcg tccgatgcac
     2701 atccaggact cggcgctcaa gatcgacatc ctgccagcgc aatgccagta actctccctg
     2761 ccggagaccc gttgctatgg cgaccgcgat cagcggcccc atacggtcgt ctttggtcgc
     2821 ctcgatcagc cggcgagctt gctccggtgt cagtggcacg attcgcgtgc gcttctctcg
     2881 ccgggcgtca gagagggccg cggcgttgct gcggaccatc tgcgccttga cggctgagct
     2941 cagggcattc accagcgtgg cgcggatgag gttcgccgtc gctggcgata cgcccttgtt
     3001 cacgatccca gcgagcatcc ggttgacgtc ggtcgccgtc agttcgcgaa gctgaatccc
     3061 accgatcgcc ggcaggatgt gattgttgat gtgaaaccgg tagttcttga gcgttgacgg
     3121 tcgcactctg ttcgccgctg tcgtctcaag ccagtcggtg aggtaatccc gcacagtcag
     3181 acgctcatca cgtcgtgaca gcccatcctc atagtcgcga agggtttgct tggctttccg
     3241 ccgggcctcg cgttcagtct tgccg
//
LOCUS       DASYWL010000255         3112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_575580, whole genome shotgun sequence.
ACCESSION   DASYWL010000255 DASYWL010000000
VERSION     DASYWL010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575580"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..520)
                     /locus_tag="VGR22_09245"
     CDS             complement(134..520)
                     /locus_tag="VGR22_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEV2128789.1"
                     /translation="MTTKTVVESDQAPSAIGPYSQAVRVGNMLYTSGVVPLDPQAMEI
                     VGSTIEEQTERVMESMKALLEDAGTGLGNVVKTTCFLDDLGNFAAFNEVYARYFGDSK
                     PARSTVEVAGLPKAAMVEIEAIALMG"
     gene            1020..2783
                     /gene="glmS"
                     /locus_tag="VGR22_09250"
     CDS             1020..2783
                     /gene="glmS"
                     /locus_tag="VGR22_09250"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEV2128790.1"
                     /translation="MCGIFGYVGQPVKVGPVVLEALRSMEYRGYDSWGVAYTTGEQPG
                     GIIKAVGRVEVGAAAGAEATAAIGHTRWATHGEVCERNAHPHLSCGGRIAVVHNGIIE
                     NADELRAGLEDVLLASDTDSEIMAHLVAHNVGRSHSLTEAVQTAFSSIEGNNAFVVLD
                     RATGEVVVVTRRLPIRLSRHDDGVVLASDPAALAGMVETAIALPDDTAVSLGTPTHVA
                     EAVVRLIAEGRGVPVPDRGERIEGGPGISMRSEIAEQPAVLRRVAADIEPVLAAVALA
                     RQAERVVFTGCGSASHAAMYAADLLVEAEVGIDGVAIPASEILGQPGRIRPGDVMVAL
                     TQSGETADVIDAVTIARERGAAVVGLINAEGSSVERLVDVAVPLLAGHERSVLATKSY
                     LAMLARGAQLVRGMTGAADGALGIWLLGEADDVQEVLGSRRTQHWIAGLAEQLAHGSS
                     ALMLTSGGHMPVAREAALKVKEGSYVHAEAVLSGELKHGVIALVEDHFPCVMLTPDEA
                     IASRLRIAAEELRSRGAAVYWLGPGAPHGLIQEQAGIVVETPSPFAQTVLMQMVALET
                     ALMRGVDPDFPRNLAKSVTVR"
BASE COUNT          508 a          919 c         1136 g          549 t
ORIGIN      
        1 ggacttgaac ctgtgacctc acggatgtga accgtgcgct ctggccagct gagctacgcg
       61 cccgtctcta cagcgaaacg agtatacacg cggggtccct ggcacgccaa cccgcgtgta
      121 tactcgtttc gcctcagccc atgagcgcga tcgcctcgat ctcgaccatc gccgccttcg
      181 gcaggccggc cacctcgacc gtggagcgtg cgggttttga gtcgccgaaa tagcgggcgt
      241 agacctcgtt gaaggccgcg aagttgccga ggtcgtcaag gaagcaggtg gtcttgacga
      301 cgttgccgag gccggtgcct gcgtcctcca gcagcgcctt catgctctcc atgacccgct
      361 cggtctgctc ctcgatggtg gatccgacga tctccattgc ctggggatcc agtggcacca
      421 caccggaggt gtagagcatg ttgccgacac gaactgcttg cgagtaggga ccgatcgccg
      481 agggtgcctg gtccgactcc acaacggttt tggtggtcat gggatctcct gcctctgaac
      541 gagagtgcgg cctgctggcc gccgaatatg ccaatcgtaa ccggtgagga tggtaccaag
      601 cctttcacct tcgagtgctt tttgtccggt gaaaacgact ggacggcggc ccgaaagagg
      661 actacgatgt acgtacgacg tggtgacggg gacagtatcc gaatcccagc gccaggactc
      721 ggcaggagac cgagttgacg aggcggaagc cgatcgaacc attcggcgga tggcttcccg
      781 gcgtggcaca gtcgtgacga gcgaggcaaa accgcgcggc gacgcgcgaa caagactccg
      841 ttcggtgtgc cggtggtcag ttcgggccac cacaccccct ccacacgtca cccgacaaac
      901 cgctgtattc gcgatgtgcc cggtataccc cgggtgtgct gcgcattgtc tggccgtttc
      961 gggcgggttc gctgcgctag cggtggggca gtgttggtca tgtccttggg aggtggaacg
     1021 tgtgtggaat ctttggatac gttggccaac cggtgaaagt cgggccggtg gtgctcgagg
     1081 cactacgttc gatggagtac cgagggtacg actcgtgggg tgtggcctac accaccggcg
     1141 agcagccggg aggcatcatc aaggcggtag gacgtgtcga ggtgggcgcc gcagcgggtg
     1201 ccgaggccac cgctgccatc gggcacaccc ggtgggcgac gcacggtgag gtgtgcgagc
     1261 gcaacgcaca cccgcacctc agttgtggcg gccgcatcgc ggtggtgcac aacggcatca
     1321 tcgagaacgc cgacgaactc cgggccgggt tggaagatgt gctgctggca tcggatacgg
     1381 actccgagat catggcccat cttgtggccc acaatgttgg ccggagtcat tcccttacgg
     1441 aggcggtaca aaccgccttc tcctctatag aaggaaacaa tgcgttcgtg gtgctcgatc
     1501 gcgcgaccgg cgaggtcgtc gtggtaacgc ggcgcctgcc gatccgcctg agccgtcacg
     1561 acgatggcgt ggtgctcgcg tcggaccccg ctgccttggc agggatggtc gagacggcga
     1621 tcgcgctgcc ggatgatacg gcagtgagcc tgggaacgcc gacgcatgtc gcggaggctg
     1681 tcgttcggct catcgccgag ggacgcgggg tcccggttcc ggatcgcggc gagcggatcg
     1741 agggcggtcc ggggatctcc atgcgttcgg agatcgccga gcagccggct gtgctgcgtc
     1801 gcgtcgcggc tgacattgag ccagtgctgg cagcagtggc gctcgcccgg caagccgagc
     1861 gtgtcgtctt caccgggtgt ggcagcgcct cgcatgcagc gatgtacgcg gcagacctgt
     1921 tggttgaagc ggaggtcggt attgacgggg ttgcgatccc agcctctgag atccttggtc
     1981 agcccggtcg catccgtccg ggcgatgtga tggtcgcttt gacgcagagt ggcgagaccg
     2041 cggacgtgat cgacgcggtg accatcgcac gcgagcgcgg tgctgccgtt gtcgggctca
     2101 tcaacgccga gggcagctcg gtcgagcgac tcgttgacgt cgccgtgccg ctgttggccg
     2161 gtcatgaacg cagcgtgctg gcgaccaagt cctatctggc gatgttggcc cgcggcgcac
     2221 aattggtccg tgggatgacc ggcgcagccg acggggcgct gggcatatgg ctgttgggag
     2281 aggcggacga tgtccaggag gttcttggca gccgtcgtac gcagcactgg atcgccggtc
     2341 tcgccgagca gctcgcgcac ggatcgagcg cgctcatgct gacgagcggt gggcacatgc
     2401 cggtcgcgcg ggaggcggcg ctcaaggtga aggaggggag ctacgtccac gccgaggcgg
     2461 tgctttccgg cgagctcaaa cacggagtga tcgccttggt ggaggatcac ttcccctgcg
     2521 tcatgttgac cccggatgaa gcgatcgcca gtcgtcttcg catcgccgcc gaggagttgc
     2581 ggagccgcgg cgcggccgtg tactggctcg ggccgggggc gccgcatgga ctcatccagg
     2641 aacaggcggg gattgtggtg gagacaccgt ccccgttcgc ccagacagtc ctgatgcaga
     2701 tggtggcgct ggagacggcg ctgatgcggg gggtcgaccc cgatttcccg cgcaatctgg
     2761 ccaagagcgt gacggtgcgg tagcgatgga gcatcaggcg tggggcgttg cttgacatcg
     2821 gggtggagcg cggctagagt tgctgggcgt tcgggcacaa gagcccgagc gccgacggtg
     2881 tccgaccggg tgcggggccg agacgctcgg catcccgcga acggatgcga cacaggccct
     2941 tgacggggcg aggcgagcgg tggtacactg gtcgccgcgc ctgaagaggg cggctcgcct
     3001 ggcggggcgc ggctcgggga tcgcacggcg gtcaccgagg cgggcttgac aggcggggag
     3061 cgagggggta gaattccttc tcggcgctgc ggttcggccg cggcgagcac ct
//
LOCUS       DASYWL010000256        10384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_13244, whole genome shotgun sequence.
ACCESSION   DASYWL010000256 DASYWL010000000
VERSION     DASYWL010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10384
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13244"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..366
                     /locus_tag="VGR22_09255"
     CDS             133..366
                     /locus_tag="VGR22_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128791.1"
                     /translation="MQHPDLIVLIQHSKQVDASRRQQTSMSHHLPPKAPGELRAAIGR
                     ALIALGTRIAPESRPAARREPLGTLPIAGCADI"
     gene            516..734
                     /locus_tag="VGR22_09260"
     CDS             516..734
                     /locus_tag="VGR22_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128792.1"
                     /translation="MQPIAPPEVILLMHREMIQAELARQRRGRPAVASRPPAQPLRVV
                     RARLGHALVAIGMRIDPSARQLPRPEAR"
     gene            790..1623
                     /locus_tag="VGR22_09265"
     CDS             790..1623
                     /locus_tag="VGR22_09265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2128793.1"
                     /translation="MTTHKHDLALDLQSVGKTFKKKRNEYAHALNGVSLELRRGECVG
                     ILGPNGSGKSTLVRVISTLTTQDTGSVRIFGHDAVKEAKTVQRYMNRVSVEASFFKKM
                     SSLENLLFGAKLYGVSDAESRPRIREILEGVGFDYKRINDPMEHLSRGMQQKIALARA
                     LLTSPVLMLLDEPTTGLDPRSKKDVQALVQRIRADHDASILLCTHDMDEAEQLCDRIG
                     ILLNGELVAIDTVQGLKQHYQKDGVLPSLEEVFMAATGSTLEEAEFADTLDDEPIAQG
                     A"
     gene            1627..2469
                     /locus_tag="VGR22_09270"
     CDS             1627..2469
                     /locus_tag="VGR22_09270"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2128794.1"
                     /translation="MQLAPVLHPKPHWKREAIAIWGYVQRNYFLTKRYFMWELVWLVY
                     SVMNALTIGFIGVGFGETLADTNRITTYLLIGTLIWSYLSMIFDLLSETVSWERWEGT
                     IEYTFMSPASRAAQLIGMGLYAVVYGIVRMIIMLGVISLFFDMSLGEANYLGALGMLA
                     ICSISLVGFGMVAAVMPLLSPEKGQQVTSIVTAVLLLVSGIYYEVGVLPGWLQPLAVI
                     SPVTYALDGIREMLLDGTSFASQWDNVWPLLIMGAIFPPLGLWLFHAGEMFAKRTGRL
                     KRSG"
     gene            complement(2509..3396)
                     /gene="thiD"
                     /locus_tag="VGR22_09275"
     CDS             complement(2509..3396)
                     /gene="thiD"
                     /locus_tag="VGR22_09275"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV2128795.1"
                     /translation="MSTNQASDPVPVMLTVAGSDPSGGAGVQADLKTATTLGVYGGAV
                     ITSLTAQNTTGVSGVLPVSASFVVEQYVSVVTDLNVSAVKIGMLGTADVAQALAEALR
                     QHPVPAVVLDPVMVATSGDRLVPDDTVDAIRQHLLPLATLITPNLPEAAALLERELSH
                     DEKAMAAAALALQEAGANAVLVKGGHLAGDTSNDVLADSDGLTWFRSHRIETANTHGT
                     GCTLSTAIASFLTYPLPLRDAVRHAKNYLTEALEAGSRQRLGSGRGPVDHLWQLRTTS
                     GIPSNGRYLQQAAPHRPVF"
     gene            complement(3393..4064)
                     /gene="thiE"
                     /locus_tag="VGR22_09280"
     CDS             complement(3393..4064)
                     /gene="thiE"
                     /locus_tag="VGR22_09280"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV2128796.1"
                     /translation="MTRREPVSLDLRVYLVTGEATSATAVETIISAAASGGVTAVQLR
                     DKDGSTTARTSAARQLASVLEPLAVPLLLNDDVEAARMAGVAGVHVGPDDLHPTQARA
                     ILGPDAVIGWSVHHIRQLSDTEAVDACDYLAAGPVWPTLTKRDTAPPLGLDGVRALRA
                     AMPRRLPLIGIGGITAENAGDLIRAGADGIAVVSAIWSAPNPAHATQRLREIVDTALA
                     ERNRR"
     gene            complement(4061..4408)
                     /locus_tag="VGR22_09285"
     CDS             complement(4061..4408)
                     /locus_tag="VGR22_09285"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF014198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV2128797.1"
                     /translation="MQTPTPSSTSRALREHQPLVQCITNYVSIDIAANVLNISDASSA
                     VVHGAREAGEFANIASAVVVNIGTPSPPWGDGMLAAVAAAGERAGERAEGAGSLRWRL
                     LDALSTLPPEACA"
     regulatory      complement(4397..4494)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4640..5575
                     /locus_tag="VGR22_09290"
     CDS             4640..5575
                     /locus_tag="VGR22_09290"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEV2128798.1"
                     /translation="MRIAAFDGGKPGVVASDDTIVEIGDLLEQYDPPGPEHYLPDLIV
                     HFDELRPELERRTEAGGGTPLSNAKLDAPFARPGKIICLMGNYREGTDRPLQRIDLFF
                     KSPESIVGDGDTVVLPPHQADIFHHEAEIAVVIGREAKDLSDDEAMDAVFGYVAFNDV
                     SARGLGRSGINSFLGKSFDTFGGFGPWIVTKDELPDPQNLHITVDVDGERRQDYSTSD
                     MERPIRELMTYISSITKLHPGDVICCGTNHQGLGSMQHGDAVTTTVEGIGSFTLHVKD
                     EHSRAWQRGVDRETAERVKASAVHPDATPTGGSGS"
     gene            5572..6465
                     /locus_tag="VGR22_09295"
     CDS             5572..6465
                     /locus_tag="VGR22_09295"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEV2128799.1"
                     /translation="MRLVLTQDDRIGVLTEGGMVDVSAAFDDIRYRTAADRMPRVMAA
                     LKDRRHRIEGVAHSGSAEAVPALQAPVPRPPKLIAAFGNYREGWDRERQPQDMFLESP
                     DSVTGPDSTVVLPRHPADIFHHEAELALVIGQRAKDLPADASTLDAVAGYTCAMDVSA
                     RGIGRVGPSRIGKSFDTFTPLGPAVVTTDEVPDPQNLRVTLTVNGEERQNYTTADMEY
                     SVVEVLAFISSYMTLVPGDVILCGTQHQGLGALQDGDHAEMTIERVGTLAVDVRDPLK
                     REWARGIDEEMATRQRAVGQS"
     gene            6554..8215
                     /locus_tag="VGR22_09300"
     CDS             6554..8215
                     /locus_tag="VGR22_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2128800.1"
                     /translation="MSTRKTNPTLKRIQSRRMNRRRAVQAGMAGAAGMAAAGIVSPAV
                     ASRRSSQFARQDPEPQRGGRLRIASTGQPAGLDMHIFNNRTVTLIAWNMYEALFTFDA
                     DYSTVPMLAEGIETSEDGLTSTIPLRQGVLFHNGEEMKAADVVASFERWMPVSSLGLA
                     IQPYLESITTVDDYTVEIVLTSPLVALPSMLARQSQGLSIHPASVLEGAGSDPLPHES
                     YIGTGPYQFVEMQADRFTLLERFDDYASVDREHSGYAGAKPAYLDELEFIPVPDEAAR
                     LAGLQSGEYHYLEEIIPDQIEVIGSDPNVTIEILPPRSYGVIIMNTVGGLTSDVTIRR
                     AAQAAISVEELAQASHGAGYFEPGPGIMLPQTIWNSDVGREHYDQGDPEKCQQLLNEA
                     GYDGKPVRILTTQEDLGDYNAAIVAQQQLEEGGFTVELEVFDEATLDEHLENDDRWEM
                     TTDSYVFRPDPVLIAAFASCTATGKWCTDEKTTLMEQLQTLQDGEERFAAFENFQQLF
                     YEQVPAIKITNQFGVAALAFDVRGLLNEMHFEIEPEFANAWLAQD"
     gene            8363..9313
                     /locus_tag="VGR22_09305"
     CDS             8363..9313
                     /locus_tag="VGR22_09305"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2128801.1"
                     /translation="MTAYILRRLLLLVPVLLIVGVVVFSLVHLTPGDPTAAMLGREAT
                     IEQKEALREQLGLNEPIPVQFVEWVAGVVRLDFGESLFMGIPVTEALLERAEPTGLLT
                     LYSLTFAILLAIPSGVIAAMRPNSLIDRLLMVTSISGAAIPSFFFGILLMLFFAVRLG
                     WLPSGGYADVAEGFWEHFRYMIMPSIALGFSGAGLLARIVRSTMLDVLNQEYIRTAYA
                     KGLREQRVVTGHALRNAMIPAMTVIGILLANMLGGAVVIETVFNIPGMGRLLVQSVVR
                     RDFPVVQAAVLVVAVIEVLVMLMIDILYVFIDPRIRYGDE"
     gene            9303..10220
                     /locus_tag="VGR22_09310"
     CDS             9303..10220
                     /locus_tag="VGR22_09310"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2128802.1"
                     /translation="MTSRAELAGSAGGTRRGRRFWFSAISPNSKYYWVRLVAENPAAV
                     VSAIVLLVIVLGAIFAPMIAPFDPNYVNPADRLSEPSGTYWFGTDDLGRDIYSRVVYG
                     ARISLLVGIAVMIGATAIGAALGLVAGYYPRVDTPLMRVMDGLEAFPSILLAIAIMAA
                     FGAAIENVILALSFVYAPNIVRLVRGTTLMNSRELYVESARVIGMGDWLIMWKYILRN
                     SLSPLIVQATFVVAFAILAEASLSFLGAGVPQDVPTWGGMVSAGQARLQQAWWASVFP
                     GIFLFITVLAINLIGDALRDALDPRTRKR"
BASE COUNT         1846 a         3280 c         3410 g         1848 t
ORIGIN      
        1 gctcttgatc agcaacacga ttgcatatcg gggccggaca cgaaccggca cgaccggaat
       61 cagcaaccgc tacaagccac cgttacccga accagatacc gcggcgcagc gccgcatgag
      121 gaccaagcag ccatgcaaca cccagacctc atcgtcctga tccagcactc gaagcaggtc
      181 gacgccagcc gtcggcagca gacgtcgatg tcacatcacc taccgccgaa agcgcctggc
      241 gagctccgcg ccgccattgg gcgagcactc atcgccctcg gaacccggat cgctcccgag
      301 agccgcccgg cagcacgtcg tgagccgctc gggacgctcc cgatcgccgg atgtgccgat
      361 atctgagtct gagtccggag cgcagcatgc cgcgctccac cacgggaccc aacgccccgt
      421 ggccgtctca tgtgatcggc cgggaggcgc ttcaaccagc gggacattga gtgttccgct
      481 gcatcgcaca gcgttgaacc aacggaggct caactatgca acccatcgca ccccctgaag
      541 tcatcctgct catgcaccgc gagatgatcc aggccgagct ggcgcgtcag cgccgaggac
      601 gaccggcagt ggcgtcacgg ccgcccgcgc agcccttgcg cgtggtacgc gcccgactcg
      661 ggcatgcgct cgtcgcaatt ggcatgcgca tcgacccgag cgcgcgacag ctgccccggc
      721 cagaggccag atgaccatcc gagccgatcc ggaagtgctc cggaccggcc acccaggaga
      781 acgacgacgg tgaccacgca caaacacgat ctcgcccttg acctgcagtc cgtcggcaag
      841 acgttcaaga aaaagcgaaa cgagtatgcc cacgcgctga acggcgtctc gcttgagctg
      901 cgacgtggtg agtgcgtcgg cattctcggg ccgaacggat cgggcaagag cacgctggtg
      961 cgcgtgatct cgacgctgac tacccaggac accgggtcgg tccggatctt cggtcatgac
     1021 gccgtgaagg aggccaagac ggttcagcgg tacatgaacc gcgtgagcgt cgaggcgagc
     1081 ttcttcaaga agatgtcgtc gctggagaat ctgctcttcg gcgcgaagct ctacggcgtc
     1141 tccgatgccg aaagtcgccc acgcatccgg gagatcctgg agggtgtcgg attcgactac
     1201 aagcgcatca acgacccaat ggagcatctc tctcgcggca tgcagcagaa gatcgcgctg
     1261 gcacgggcgc tgctcaccag cccggtgctg atgctgctcg acgagccgac gacgggtctc
     1321 gacccgcgca gcaagaagga tgtgcaggcg ctggtccagc gcatccgggc ggaccatgac
     1381 gcctcgatcc tgctctgcac ccacgacatg gacgaggctg agcagctctg cgaccgcatc
     1441 ggcatcctgc tcaacggcga gctggtggca attgacacgg tgcaggggct caagcagcac
     1501 taccagaagg acggcgtgct gccgtccctt gaggaggtct tcatggcggc gaccgggtcg
     1561 acgctggagg aggccgagtt cgcggacacg ctcgatgacg agccgattgc gcaaggagcc
     1621 tgatcgatgc aactcgcacc cgtgttgcac ccgaagcctc attggaagcg cgaggcaatc
     1681 gcgatctggg gctacgtaca gcgcaactac ttcctgacga agcgctactt catgtgggag
     1741 ctcgtctggc tggtctactc ggtgatgaac gccctgacga tcgggttcat tggtgtcggc
     1801 ttcggcgaaa ccctagcgga tacgaaccga atcaccacct acctcctgat cggcacgctg
     1861 atctggagct acctctcgat gatcttcgac ctgctgtcgg agactgtgag ctgggagcgg
     1921 tgggaaggca ccatcgagta cacgttcatg tcgccggcga gccgcgcggc gcagctgatc
     1981 ggcatggggc tctacgcggt cgtctacggg atcgtccgca tgatcatcat gctgggcgtc
     2041 atctcgctct tcttcgacat gagcttggga gaagcaaact acctcggcgc gctgggcatg
     2101 ctggcgatct gcagtatctc cctggtcggt ttcggtatgg tggctgctgt catgccgctg
     2161 ctctcgccgg agaagggcca gcaggtaacg agcatcgtca cggcggtgtt gctactcgtc
     2221 tcgggcatct actacgaggt gggtgtcctg ccgggatggc tgcagccgct cgcggtgatc
     2281 tcgccagtca cctacgcgct ggacggcatc cgcgagatgc tgctggacgg cacctcgttc
     2341 gccagccagt gggacaacgt ctggccgctg ctgatcatgg gcgccatctt cccgccgctg
     2401 ggcctctggc tcttccacgc gggcgagatg ttcgccaagc ggaccggccg gctcaagcgc
     2461 agcgggtagc ataacgcgcg aggcaatgat cttccgcccg gtgtgtgctc aaaacaccgg
     2521 gcggtgcggc gcagcctgtt gcaggtatcg gccattgctc gggatgccgg acgtggtcct
     2581 gagctgccag agatgatcga ccgggccacg acccgagccg aggcgctggc gtgatccggc
     2641 ctcaagcgct tcggtgaggt agttcttggc gtgccggacc gcgtcgcgaa gcggcaaggg
     2701 ataggtcagg aagctggcga tcgctgtcga gagcgtgcac cccgtgccgt gcgtgttcgc
     2761 agtctcgatg cgatgcgagc ggaaccaggt cagcccgtcg gagtcggcga gcacgtcgtt
     2821 gctggtgtcc ccggcaagat ggccgccctt gaccagcacg gcattcgccc cggcctcctg
     2881 gagcgccagc gccgcggctg ccatcgcttt ctcgtcgtgc gagagctccc tctcgagaag
     2941 cgcggcggct tcggggaggt tgggcgtgat cagcgtcgca aggggcagca ggtgctggcg
     3001 aatggcatcg accgtatcat cggggaccag gcggtccccg gaggtggcca ccatgaccgg
     3061 gtcgaggacc actgccggca ccggatgctg acggagtgcc tcggccagtg cctgggcaac
     3121 gtcggcagtg ccgagcatcc cgatcttcac cgcgctcacg ttcaagtcgg tgacgaccga
     3181 gacatactgc tccacgacga acgatgcgga gacgggcaag accccgctga cccctgtggt
     3241 gttctgggca gtgagcgagg tgatgaccgc accaccatag acgcccaatg ttgtggcggt
     3301 cttgaggtcg gcctggacgc cagcgcctcc ggaggggtcc gatcccgcca cggtcagcat
     3361 gacgggcacg gggtcggatg cctggttggt gctcatcggc gattcctttc ggcgagggcg
     3421 gtatcgacga tttcgcgcag cctttgggtg gcatgagcgg gattgggcgc gctccaaatc
     3481 gcggacacga ccgcgatacc gtcggcgccc gcccggatga gatcccccgc attctcggct
     3541 gtgatgccgc cgataccgat cagcggaagc cgacgaggca ttgccgcgcg caaggcgcgc
     3601 accccgtcga gacccagcgg cggggccgtg tcacgcttgg tcagggtggg ccagaccggg
     3661 cccgcagcca gatagtcgca ggcatcaacg gcttccgtgt cggagagctg tcggatgtgg
     3721 tggaccgacc agccgatcac cgcgtccggt cccaggatcg ctcgggcctg tgtcgggtgc
     3781 agatcatcag ggcccacgtg aacgccggca accccggcca tccgagctgc ttcgacgtcg
     3841 tcgttcagca acaacggcac cgccaacggc tccagcaccg acgccagttg tcgcgcggcg
     3901 gatgtccgtg cggttgtgct cccatccttg tcgcgcaact gcaccgctgt aaccccaccg
     3961 gaagcggctg cggagatgat tgtctcgact gcggtggcag aggtggcctc gccggtgacc
     4021 agatagacgc gcagatcgag gctcaccggc tctctacgtg tcaagcgcac gcctccggtg
     4081 ggagcgtgga cagtgcgtcg agcagccgcc agcgcagcga tcccgccccc tcggcacgct
     4141 cgcccgcccg ctccccggcg gcggcaacgg cggccagcat gccatcgccc catggcggcg
     4201 agggagtgcc gatgttgacg accacggcgg acgcgatgtt ggcgaactcg ccggcttcac
     4261 gggcgccgtg caccactgcg gaagacgcat cggagatatt caacacgttc gcggcgatgt
     4321 cgatggaaac atagttggtg atgcactgca ccaggggctg gtgctcgcgc aacgcacgag
     4381 aggtggaaga gggggttggg gtctgcatgc gggctccctt gcgccggtat gatccggatc
     4441 aggttcggcg ggtctcatct cagctccctc gcgagagcac cccggccgcg ttgtcggcaa
     4501 gtatagggca tgctgcggag tttggcgtgt tccagcgctc gatagcgaaa ccctgttgac
     4561 agcgaatcgg cgttgtgtga ctatctcgcc acttgcacaa ctgatctgct cgccgaggcg
     4621 aggagcgaga ggaggcgtga tgcgtattgc agcgttcgac ggcggaaagc ccggcgttgt
     4681 tgcgtccgac gatacgatcg tcgaaatcgg ggacctgctc gaacagtacg atcccccggg
     4741 acctgagcat tacttgcccg acctcatcgt ccacttcgac gaactccgcc ctgagctgga
     4801 gcgccgcacc gaagcgggtg gtggcacacc gctgagcaat gcgaagctcg atgcgccctt
     4861 tgcgcgcccg ggcaagatca tctgcctgat gggcaactat cgcgagggga ccgatcgacc
     4921 gcttcagagg atcgacctct tcttcaagtc accggagagc atcgtcgggg acggcgacac
     4981 ggtggtcctg ccgccgcatc aggccgacat cttccaccac gaagcggaga tcgccgtggt
     5041 gattggccgc gaggcgaagg acctctccga cgatgaggcg atggatgccg tgttcggcta
     5101 cgtggcgttc aacgacgtct cagcgcgtgg gctcgggcgc tccggcatca acagcttcct
     5161 tggaaagtcg ttcgatacct tcggcggatt cggtccgtgg atcgtgacga aggacgagct
     5221 gccggatccg cagaacctgc acatcactgt cgacgtcgac ggtgagcgcc gacaggatta
     5281 ttccacaagt gacatggagc ggccgatcag ggaactcatg acctacatct cctcgatcac
     5341 caagctgcac cccggcgatg tcatctgctg cggcacgaac caccaggggc tcggctccat
     5401 gcagcacggt gacgccgtga cgaccacggt cgaagggatc ggctcgttca cgctgcacgt
     5461 gaaggacgag cacagtcgcg cttggcagcg gggggtcgac agggagacgg cggagcgggt
     5521 gaaagcctcg gctgtgcatc cggatgcgac cccgaccgga ggttctggct catgagactc
     5581 gtgctgacac aagacgatcg catcggggtc ctgacggaag gtggtatggt cgatgtctcc
     5641 gccgccttcg acgacatccg gtatcgcacc gccgccgacc gcatgcctcg ggtgatggcg
     5701 gcactgaagg atcgtcgcca ccggatcgaa ggtgtggcac acagcggatc ggccgaagcg
     5761 gttcccgcgt tgcaggcccc ggtacctcgt ccaccgaagt tgatcgcggc gttcggaaac
     5821 taccgcgagg gatgggatcg tgaacgccag ccccaggaca tgttcctgga gagcccggac
     5881 tccgtgacgg ggcccgacag cactgtagtg ctgcccagac atcccgccga catcttccac
     5941 catgaagctg aactggcgct ggtgatcggc cagcgcgcga aggacctgcc ggccgacgcg
     6001 tcgaccctcg atgccgttgc cggttacacc tgcgcgatgg acgtctccgc tcgcggcatc
     6061 ggtcgcgtcg gcccgagccg catcggcaag tcgttcgata ccttcacgcc gctcgggcca
     6121 gcggtcgtga cgacagatga ggtccccgac ccgcagaacc tgcgggtgac actcacggtc
     6181 aacggcgagg aacgccagaa ctacacgacc gccgacatgg aatacagcgt ggtggaggta
     6241 ctggccttca tcagctcgta catgacgctg gtgccgggag acgtgattct ctgtggcacc
     6301 cagcaccagg ggcttggtgc cctgcaggat ggggaccacg ccgagatgac catcgagcgg
     6361 gtcggcacac tggcggtcga tgtccgcgac ccgctcaagc gcgagtgggc gcgaggcatc
     6421 gatgaggaga tggccacacg ccagcgggcg gtcggccagt cgtagggagg gtgtaccgag
     6481 cagagcagag accgtatcgt cgcgtattgc cgaagatttc tcaaacgaca ccagctcacg
     6541 agcaggaggc accatgagca cgcgcaagac gaacccaacg ctgaaacgca tccagtcgcg
     6601 tcgcatgaac cgccgccggg cagtgcaggc aggcatggcc ggtgcagccg gaatggctgc
     6661 agccggcata gtgagtcccg ctgttgcgag caggcgatct tctcaattcg ctcgacagga
     6721 ccccgagccg cagcgcggtg gacgattgcg gatcgcctcc actgggcaac cggccggtct
     6781 tgacatgcac atcttcaaca accgcaccgt gacgctcatc gcctggaaca tgtacgaggc
     6841 gctcttcacg ttcgacgccg actactccac cgtgccgatg ctcgccgagg gtatcgagac
     6901 gagcgaagat ggcctgacca gcacgatccc gctccgccag ggtgtgctct tccacaacgg
     6961 tgaggagatg aaggccgccg atgtcgtcgc ctcgttcgaa cgctggatgc cggtgagcag
     7021 tcttggtctc gcgatccagc cgtacctcga gtcgatcacc actgttgatg actacaccgt
     7081 cgagatcgtc ctgacgagtc cgctggtggc gctgcccagc atgctggcga ggcagtccca
     7141 gggactctcg atccatccgg catcggtact ggaaggcgcc ggctcggatc cgttgccgca
     7201 cgagagctac attggcaccg gtccgtatca gttcgtcgag atgcaggcag accgattcac
     7261 cctgctggag cgcttcgacg attatgccag cgtggatcgc gagcacagcg gttacgcggg
     7321 agccaagcct gcatacctcg acgagctcga gttcattccg gtgccggacg aggcagcccg
     7381 cctggctggc ctgcagtctg gggagtacca ctatctggag gagatcatcc cggaccagat
     7441 cgaggtcatc gggagcgatc ccaacgtgac catcgagatc ctgcccccgc gcagttacgg
     7501 cgtcatcatc atgaacacgg tgggtggtct cacgagcgat gtgacaatcc gccgggctgc
     7561 acaggcggcg atttctgtgg aggagctcgc acaggcgtca catggtgcag ggtacttcga
     7621 gcctggccct ggcattatgc tgccgcagac catctggaac tcggacgtcg gtcgagagca
     7681 ctacgaccag ggtgaccctg aaaaatgcca gcagctcctc aatgaggcgg ggtacgacgg
     7741 caagcctgtc cggatcctga ccacgcagga ggatctcggt gactacaacg ccgccatcgt
     7801 cgctcagcaa cagctcgagg agggtgggtt cactgttgag ctggaggtct tcgacgaggc
     7861 gacgctcgat gagcatctcg aaaatgacga ccggtgggag atgacgactg actcgtatgt
     7921 cttccggccc gatccggttc tcatcgcggc gttcgcgagc tgcaccgcca caggcaagtg
     7981 gtgcacggac gagaagacca ctttgatgga gcagctgcag actctgcagg acggcgaaga
     8041 gcgcttcgcg gcctttgaga acttccagca gctcttctac gagcaggtgc cagccatcaa
     8101 gatcacgaat cagttcggcg tggccgccct ggcattcgac gtcagggggc tgttgaacga
     8161 gatgcacttc gagatcgagc cggagttcgc gaacgcctgg ctcgcgcagg actgatgcct
     8221 gaggggcgcg gtgtttccag ccgtgcccct cgccgtgatg tgccgatgtt cgaggtgtac
     8281 gagccggtca cgtacggcat gtcgtacgtg accggcgcat tggctagcga acactgatct
     8341 tgtacgcgtc gggagactcc gcgtgaccgc ttatatcttg cgtcgcctgc tcctcctcgt
     8401 gccggttctc ctgatcgtcg gggtggtcgt cttcagcctg gtgcacctga caccgggcga
     8461 tccgacggca gcgatgctcg ggcgcgaggc gacgatcgag cagaaggagg cgctgcggga
     8521 gcaactcggc ctcaacgagc cgatcccggt ccagttcgtc gaatgggtcg cgggggtcgt
     8581 tcgcctcgac ttcggtgagt cgctcttcat gggtataccg gtgaccgagg cgttgctcga
     8641 gcgtgccgag ccgacagggc tgcttacgct ctactcgctg acgttcgcga tcctgctggc
     8701 gattccctcc ggtgtcattg cggcaatgcg gccgaactcg ctgatcgacc gactgctgat
     8761 ggtgacgtcg atcagcggcg cagctatccc gagcttcttc ttcggcatcc tgctcatgct
     8821 cttcttcgcg gtccggctcg gctggctgcc gtcaggtggg tatgccgatg tcgctgaggg
     8881 gttctgggag cattttcgct acatgatcat gccgtcgatc gcgctcgggt tctccggtgc
     8941 cggtctgttg gcacgtatcg ttcgctctac gatgctcgac gtgctcaacc aggagtacat
     9001 ccgcaccgcc tacgcgaagg gactgcgcga gcagcgggtg gtgaccgggc atgccctgcg
     9061 caacgcgatg atcccggcga tgaccgtcat cggcatcctg ctcgccaaca tgctgggcgg
     9121 cgcggtcgtg atcgagacgg tgttcaacat ccccggtatg ggacggctac tggtccagag
     9181 cgtcgtccgt cgcgattttc cggttgtcca ggcggcggtg ctcgtcgtgg cggtcatcga
     9241 ggtgctggtg atgctgatga tcgatattct ctacgtcttc atcgatccga ggatccggta
     9301 tggtgacgag tagggcggag ctggccggca gtgccggtgg aacacggcgt ggacggcgat
     9361 tctggttctc ggcgatctcg cccaactcca agtactactg ggtccgtctg gtggcggaga
     9421 acccggcggc agttgtcagc gcgatcgtcc tgctcgtcat tgtgctcgga gcgatcttcg
     9481 caccaatgat tgcgccattc gatccaaact acgtcaaccc tgccgaccgc ctcagcgagc
     9541 cgtccggtac ctactggttc gggacggatg acctcggccg cgatatctac agccgcgtcg
     9601 tgtatggcgc gcgaatctcg ctgctcgtcg gaatcgccgt gatgatcgga gcgaccgcga
     9661 tcggcgccgc gctgggcctc gtggcgggct actacccgcg cgtcgacaca ccgttgatgc
     9721 gtgtcatgga cggccttgag gcgtttccca gcatcctgct ggccatcgcg atcatggcgg
     9781 cgttcggcgc agcgatcgag aacgtcatcc tcgcgctgtc gttcgtctac gcccccaaca
     9841 tcgtccgact cgtgcgtggc acgaccctga tgaacagtcg cgagctctac gtcgagtcgg
     9901 cccgtgtgat cggcatgggc gactggttga tcatgtggaa gtacatcctg cggaactcgc
     9961 tctcgccgct gatcgtgcag gcgacattcg tcgtcgcgtt cgccatcctc gccgaagcct
    10021 cgttgtcgtt tctcggcgcg ggcgtgccac aggacgtccc gacgtggggc ggcatggtca
    10081 gcgcagggca ggcaaggctg cagcaggcct ggtgggcatc cgtcttcccg ggcatcttcc
    10141 tcttcatcac ggtgctggcg atcaacctca tcggtgacgc cctccgcgat gccctggatc
    10201 cccgcacccg caagcggtag agcgatcagg aagctcccct gatctgtcac gggtggcttc
    10261 cggtcagcgg tgctgcggtc gatcgatggt gagcaggtag gcgaggccgt ccggagtgac
    10321 gatcgggtcg gccgtgggat cgatgatggc catctcccct tgccggagct ggtcgatggt
    10381 ccgc
//
LOCUS       DASYWL010000257         5957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_463548, whole genome shotgun sequence.
ACCESSION   DASYWL010000257 DASYWL010000000
VERSION     DASYWL010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5957
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463548"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VGR22_09315"
     CDS             complement(<1..261)
                     /locus_tag="VGR22_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128803.1"
                     /translation="MAKSTVPPQSTTAGGQPADNRAIMRANKTRIIGAQALQNAGDQV
                     VKASTVLTWLLAALGAPTWTIGLLVPVRESGSMLPQAALTPWV"
     gene            452..1396
                     /locus_tag="VGR22_09320"
     CDS             452..1396
                     /locus_tag="VGR22_09320"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV2128804.1"
                     /translation="MSLEPSALYGVIPPISTPLTPERTLDVASFRRLIDRQVGAGIHG
                     IFVLGSTSEAPLLPNAMQDAVIGTAVEHVSGRIPVIAGCIDLTTARVIDRGTRARTLG
                     ADGIVVCPPFYINPAQQEIIRHYRMVHEAVGLPILAYDIPSATHVKLQRPVLKALADD
                     GTIMGLKDSSGQDANFRGVILDNTQNQDFRIFTGSELTADNAMFMGADGLVPGLGNVD
                     PAGYVRLYDACVTGNCVAARAEQERLYRLSGISAVAMHGDYGMSAAAHGAFKAAIWLQ
                     GHIDHPLTMYPHTELTRDHHRQIAAILADCGLEVTREA"
     gene            1499..1948
                     /locus_tag="VGR22_09325"
     CDS             1499..1948
                     /locus_tag="VGR22_09325"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HEV2128805.1"
                     /translation="MELSGEHRFSAPRERVYDFLLDPDTLQACLPGCEKLEQVGEDEY
                     SATMKIGIGMIRGTFTGKVKISDKQEPSSYKMLVEGRGPQGQVSGEGSLELTEDGDGT
                     IVHWSGDANVRGTLARIGSRVIKPAATTIVGQFFDCLESRATATQQA"
     gene            2003..2521
                     /locus_tag="VGR22_09330"
     CDS             2003..2521
                     /locus_tag="VGR22_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEV2128806.1"
                     /translation="MSSETISVSTKVNGTEQTIDVEPRTLLVHMLRENLNLTGTHTGC
                     ETGQCGACTVLMDGRAVKSCMVLAAQAEGREVTTIEALAESEHNLHPLQEAFWEEHGV
                     QCGYCTPGMILSTLALLSDTPHPDEGQIRHAIEGNICRCTGYNSIVKAVQVASERLGA
                     DDLAVAGTKAEG"
     gene            2524..3381
                     /locus_tag="VGR22_09335"
     CDS             2524..3381
                     /locus_tag="VGR22_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEV2128807.1"
                     /translation="MYPAPFTYHRATSVSEALSLLQEHADTGKLLAGGHSLLPIMKLR
                     LAQPEHLIDISGLEELRQITFGEDVVTIGALVTHRELATNAELAARVPLFAEMANVIG
                     DQQVRNLGTIGGVVAHADPAADYPAGMLVLDVTVLATGSNGEREIPIGEFFQGFLTTA
                     LAPDELLTGVRVPLPASGTNSAYEKLANPASGYATVGVAAVVSRGDDGTIGDIRVGIT
                     GASDTAWRATSVEDALRGTSPDPAAIKAAAASTTEGVEMLEDKHAPADYRARVTRNLV
                     RRAIERALA"
     gene            3650..4552
                     /locus_tag="VGR22_09340"
     CDS             3650..4552
                     /locus_tag="VGR22_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128808.1"
                     /translation="MHANTSINDRRRHRGANPLVWIAGAIGLIIVALALVWLIAGTDT
                     VLGWFGLADDAQSVGDESGVNLSEVSDNPDAMYGHTVSVSGGVQEVVSPHAITIGSDQ
                     IIVGDTVLVVSAGDLPALLEAEVGDEPDDIALLVTGILRPFDREAIAEEVGVALDDEE
                     LDYDGEAVIVAQEIEVNPPNLGPGDTERQGPSAGFDVGTDISDIVENTEAYVGETVTV
                     SGEVERVINPHAFLFGDAMVLAISAEERDNIFVEPTAYIRGTVQMFDQVEIEEQLGLD
                     LDDEQFAEFDGKPVVIVEALDLVK"
     gene            complement(4621..5127)
                     /locus_tag="VGR22_09345"
     CDS             complement(4621..5127)
                     /locus_tag="VGR22_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128809.1"
                     /translation="MADDRTRDRVEGVVPGRGFPQTPHELPIDRIRVDALLDRVRQGA
                     RISLIDEFLNCVDWRGAFAGNDGKPLDTEEVLRLMAYYREKFADIGPIYLAELLSTEF
                     MTELRAQGDIQFSERLLEIGRNEPELWKEIRLFFRRKEFATALLVHAEMEESDKIEPT
                     WEGAEPNA"
     gene            5346..>5957
                     /locus_tag="VGR22_09350"
     CDS             5346..>5957
                     /locus_tag="VGR22_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEV2128810.1"
                     /translation="MANGRISRSNQRHAAARRRLSRYGNSRRGQHHRKLPPNLLRHSS
                     GRNGNGKKGAAWAPRIAIALGALGLFSVLAFVVITVITSVLGVTGTVAAYRQVNGELP
                     NAAEIAVETFQTTRIYDRNGTLLQEVDNPDYGWRTFVSLDQVSPYLVDATVAAEDATF
                     WTNPGIEPAGIARAAVINVSGTGSSGASTITQQLVRSIYPEKIG"
BASE COUNT         1064 a         2022 c         1922 g          949 t
ORIGIN      
        1 cacccacggc gtcagggcgg cctgcggcag catcgagccc gattcgcgga ctggcacgag
       61 caggccgatg gtccacgtcg gcgcgccgag tgccgccagc agccaggtga gcacggtgga
      121 ggccttgacc acctggtcgc cggcgttctg gagcgcctgc gcgccgatga tacgcgtctt
      181 gttggcgcgc atgatggctc ggttgtcggc gggctggccg ccagctgtgg tggattgtgg
      241 cggtacggtc gacttggcca tgatgcccct ccgggtttcc ccctcgtggg gaagtatcag
      301 acaggatcgc catcatgatc gaccctgtgg agcgcctggc agtggcgaca acccgagacg
      361 gacatggcac agtgcctggg cgtgcttcgc atatacgcag cgcgcgccgg caccggtgtc
      421 gacctcccga ccactatcag aaagggcact catgtcactc gaaccctcgg cgctgtacgg
      481 cgtcatcccg ccgatcagca cgcccctcac tccggagcgc acgttggatg tcgccagctt
      541 ccgtcgattg atcgaccgcc aggtcggcgc cggtatccat gggatcttcg tgctcggctc
      601 gaccagcgag gcccccctgc tgcccaacgc catgcaggat gcagtgatcg gcactgccgt
      661 cgagcacgtc agcggccgca tcccggtcat cgccgggtgc atcgacctca ccaccgcacg
      721 cgtgatcgat cgcggcaccc gggcacgcac gctcggcgcc gacggtatcg tggtctgtcc
      781 accgttctac atcaatcccg ctcaacaaga gatcatccgg cactaccgga tggtccacga
      841 ggcagtcggg ctgccgatcc tcgcctacga catcccttcg gcgacccacg tcaagcttca
      901 gcgtccagtg ctgaaggcgc tcgctgacga cggcacgatc atgggactca aggacagcag
      961 cgggcaggac gccaacttcc gtggggtgat cctcgacaac acccagaacc aggacttccg
     1021 gatcttcacg ggttcggaac tgactgccga caacgcgatg ttcatgggcg cggacggtct
     1081 cgtgccgggt ctcggcaatg tcgaccccgc cggctacgtc cgcctctacg acgcctgcgt
     1141 cactggcaac tgcgtcgccg cacgcgctga gcaggagcgc ctgtaccggc tctcgggaat
     1201 cagcgccgtg gcgatgcacg gtgactatgg catgtcggca gctgcccatg gcgcgttcaa
     1261 ggccgcgatc tggctgcagg gacacatcga ccaccctctc accatgtacc cgcacaccga
     1321 gctgacccgc gaccaccacc gccagatcgc ggcaatcctc gccgattgcg ggcttgaggt
     1381 cactcgggag gcgtagcggt tcggcaccgt ttgtgcgacc atgctgcgca gttagggcca
     1441 cggcatctcg accgatacag cggtcgcgtg ccgtccgcac gaaaccagga agggaaccat
     1501 ggaactatcc ggcgagcacc gcttctcggc cccgagggag cgcgtctacg attttctgct
     1561 cgaccccgac acgctgcagg catgcctgcc gggttgcgag aagctcgagc aagtcggcga
     1621 ggacgagtac agtgcgacga tgaagatcgg gatcggcatg atccgcggca ccttcaccgg
     1681 caaggtgaag atcagcgaca agcaggagcc cagcagctac aagatgctgg tcgagggccg
     1741 cggcccgcag ggccaggtct cgggcgaggg gtcgctggag ctcaccgagg acggcgacgg
     1801 cacgattgtg cactggagcg gcgacgctaa cgtgcgcggc acgctcgctc gcatcggcag
     1861 ccgcgtcatc aagccggccg ccacgaccat cgtcgggcaa ttcttcgact gtctggaatc
     1921 cagggcaacg gccacgcagc aggcctgagg tttgtccggc acgccggaca gcctgccgcc
     1981 actcacatga ggagctacgt acgtgtcatc cgagaccatc tccgtctcta cgaaggtcaa
     2041 cgggacggaa cagactattg atgtggaacc ccggaccctg ctggtgcaca tgcttcgtga
     2101 gaatctcaat ctcactggca cgcacaccgg ctgcgaaacc ggccagtgtg gcgcctgtac
     2161 cgtgctgatg gacggcaggg ccgtgaagag ttgcatggtg cttgccgcgc aggccgaggg
     2221 ccgcgaggtg acgaccatcg aggcgctcgc agagtccgag cacaacctcc acccgctgca
     2281 ggaggccttt tgggaggagc acggggtgca gtgcggctac tgcaccccgg gcatgatcct
     2341 ctccactctg gcgctgctga gcgatacccc gcacccggac gaggggcaga tccgtcatgc
     2401 gatcgagggc aatatctgcc gctgtaccgg ctacaacagc atcgtcaagg ccgtgcaggt
     2461 cgcctccgag cgactgggcg cggatgacct ggccgtcgcg ggcacaaagg cggagggctg
     2521 accatgtacc cggcaccatt cacctaccac cgcgcgacat cggtgagcga ggcgctctcg
     2581 ctgctgcagg agcacgccga caccggcaag ttgctggccg gcgggcacag cttgctcccc
     2641 atcatgaagt tgcggctggc ccagcccgag cacctcatcg acatctcggg actcgaagag
     2701 ctcaggcaga tcacgttcgg cgaggacgtc gtgacgatcg gcgcgctcgt cacccatcgg
     2761 gaactcgcca ccaacgccga gctggcagcg cgcgtgccgc tgttcgccga gatggcgaac
     2821 gtcatcggcg accagcaggt gcgcaatctc ggcacgatcg gtggcgtcgt ggcgcatgcc
     2881 gatcctgccg ccgactaccc ggccggcatg ctcgtactcg acgtgacggt gctcgccact
     2941 gggtcgaacg gcgagcggga gatccccatc ggcgagttct tccaggggtt cctgaccacc
     3001 gcgctcgcgc cggacgaact gctcaccggg gtgcgcgtgc ccctgccggc ctccggcacc
     3061 aacagcgcct acgagaagct cgcgaacccg gcatctggct acgcgacggt cggggtggcc
     3121 gcggtggtga gccgcggcga cgacggcacg atcggcgaca tccgtgtcgg catcaccggc
     3181 gccagcgata cagcttggcg agcaaccagc gtcgaagatg ccctccgagg cacctctccc
     3241 gacccagcgg caatcaaggc ggccgcggcg tcaacgacgg agggagtgga gatgctggag
     3301 gacaaacatg ccccggccga ttaccgggcg cgggtcaccc gcaacctcgt ccgtcgcgcc
     3361 atcgagcgtg ccctggcgta gcgatcgaac ccgaacggca ctccagcagc catgcgaccc
     3421 ccacgcgaga cgtgggggtc cggttttcgg cttgggcacc agtggtcagc gctcggccat
     3481 gcccagatgg tcgccaggcg tcggcgcggt tcaccaccga gtgagtcgag gtgtgacgga
     3541 cggcggttga agagtgtact ggcacgtatc gtgttccaat ccgcgtcgtg ccgggggagc
     3601 gccgccttcc ccggtcgttt catgatttat gatggccaga gggcaggaca tgcacgcaaa
     3661 caccagcatc aatgatcgcc gccgacatcg cggggcgaac ccgctcgtgt ggatcgcggg
     3721 tgccatcggt ctcatcatcg tcgccctcgc gctggtctgg ctgatcgccg gaaccgacac
     3781 ggtcctgggc tggttcgggc tggccgacga cgcgcaatca gtcggtgacg aatccggcgt
     3841 gaacctcagc gaggtctcgg acaacccgga cgcgatgtac ggacatacgg tctccgtcag
     3901 cggcggggtg caggaagtcg tgtcgcccca cgcgatcacg atcggtagcg accagatcat
     3961 cgtcggcgac acggtgctgg tcgtcagcgc cggggacctt ccggcgctgc tggaggccga
     4021 ggtcggcgac gagccggacg acatcgcgct gctcgtcacc ggcatcttgc gaccgttcga
     4081 ccgcgaggca atcgcggagg aggtcggtgt tgccctggac gacgaggagc tcgactacga
     4141 cggcgaggcg gtgatcgtag cccaggagat cgaggtcaac ccgccgaatc tcgggccggg
     4201 tgacaccgag cggcagggcc cgtcggccgg gttcgacgtc ggcacggaca tcagcgacat
     4261 cgtcgagaac accgaggcct acgtcggcga gaccgtgact gtcagcgggg aggtcgagcg
     4321 ggtgatcaac ccgcacgcct tcctgttcgg agacgcgatg gtgctcgcaa tcagtgccga
     4381 ggaacgtgac aacatcttcg tcgagccaac ggcctacatc cgggggaccg tgcagatgtt
     4441 cgaccaggtc gagatcgagg agcaactcgg gcttgacctc gacgacgagc agttcgccga
     4501 gttcgatgga aagcccgtgg tcatcgtcga ggcgctcgat ctggtgaaat agtcccctcc
     4561 gttctcggca agctcagcgc tgtcgggcac ggagcacaac gccgcaggac cggcaccgag
     4621 ctacgcgttc ggttccgcgc cctcccaggt cggctcgatc ttgtccgatt cctccatctc
     4681 ggcgtggacg agcaatgccg tcgcgaattc cttgcggcgg aagaagagac gaatctcctt
     4741 ccacagctcc ggctcattgc ggccgatctc cagcagccgc tcagagaact gaatatcgcc
     4801 ctgcgcgcgc agctcggtca tgaactcggt cgagagcagc tcggcgagat agatggggcc
     4861 aatgtcggcg aacttctccc ggtagtaggc catcaagcgc agcacctcct cggtgtcgag
     4921 cggcttgccg tcgttgcccg cgaacgcgcc acgccagtcc acgcagttca gaaactcgtc
     4981 gatgagggag atgcgcgcgc cctgccgcac gcggtcgagc agcgcgtcga cccggatgcg
     5041 gtcgattggc agctcgtgcg gggtctgtgg gaatccccgc ccgggcacca cgccctcaac
     5101 gcggtcgcgt gtcctgtcgt cagccattgc atctcctcgg tatcgctaca ggcacttatg
     5161 atctcagcgg gtggttgcac accgcgatga ccctaccacg ccaccaagcg gcaccggtcg
     5221 ctctggtacc attcgagggt gtatgctcgg aacctccggg attccgcgtg catccagaca
     5281 tgacacccgg cacctgcgtg ccaccttcct cacacctgca ctgcccgata tcgcccggag
     5341 gatacatggc gaacggacgc atcagccgat ccaaccagcg acacgccgcc gcccggcgac
     5401 gcctctcacg gtatggcaac agccgccgcg ggcagcatca ccgcaagctg ccgcccaacc
     5461 tcctgcgtca cagcagcggt cggaacggca atggcaagaa gggcgcggcg tgggccccac
     5521 gcatcgcgat cgcgctgggc gcgctcgggc tcttctccgt actcgccttc gtggtgatca
     5581 cggtcatcac ctcggtcctc ggtgtcaccg gcaccgtcgc tgcctaccgc caagtcaacg
     5641 gcgagctgcc gaacgccgcc gagatcgcag tcgagacctt ccagaccaca cgcatctacg
     5701 accgcaacgg taccttgctg caggaggtcg acaacccgga ctacggatgg cgcaccttcg
     5761 tctcgctcga ccaggtttcg ccctacctcg tcgacgccac ggttgccgcc gaggatgcga
     5821 ccttctggac caatcccggc atcgagccgg ccggcatcgc ccgcgccgcg gtcatcaacg
     5881 tgagtggcac cgggtcatcc ggcgcgtcca cgatcacgca gcagctggtt cgctccatct
     5941 accccgagaa gatcggc
//
LOCUS       DASYWL010000258         3170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_538112, whole genome shotgun sequence.
ACCESSION   DASYWL010000258 DASYWL010000000
VERSION     DASYWL010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538112"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..1789)
                     /locus_tag="VGR22_09355"
     CDS             complement(86..1789)
                     /locus_tag="VGR22_09355"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HEV2128811.1"
                     /translation="MNGMPLNLATPAVSGPPDTMPDISRSLRWRDWSDGRMEARQRGV
                     PILAFAEPRWSNSAQRLAYVLERDDELQDVLQARVVPVLVDWTERPDLAARWRWAAVV
                     LTGTSGPPLTILLTPDGEPMLVYPGMQYEGDDLLPSLGSLITATADAYAENAAAFDAE
                     ARSLGQADARNAGDVAGFPAFWAGLQPDLDMVRGGLHESPRHPHPQLLWTALDHVVSV
                     AAKEVVGWVTATLRHMADGGINDHLDRGFHRCSRDERWVVPHFEKPVPLNAQLTAVYA
                     RAGHELDHPEFTELAQALATFCMTALRENVDCVGSNSPYYTWTSKELLDGLNVDEVQP
                     MSLHFGIGPSPLRQVVHHAVPVERLDQYSREDLEVLRARVERGRKHMLTLRQQRPAPE
                     LVTMGGWSWRAETLRWLFVASEWLPGLELGRLQELLAIALDSGMDPQRGYVRADGNAW
                     LEDQAALLGAMVAAGRKVNHDEWHQRARALADAVVQAYRVEDGWCDRAGDDVTSKAIV
                     DDILPAPIATLTASLVALDAPDNRYTTIARTQASPYRSAASMVGPRGAAFWRSWATTA
                     L"
     gene            complement(1878..>3170)
                     /locus_tag="VGR22_09360"
     CDS             complement(1878..>3170)
                     /locus_tag="VGR22_09360"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEV2128812.1"
                     /translation="EYYIGVGGFPGNIQGEIRPVNPEEGNPFEAPRERDYPFRPLRDN
                     ATDLTFREGALELGLNPFHVPTAITTEPYTNPDGVSRPACSYCTFCTGHGCWNHSKSS
                     TLSALLPTAEETGNFELRTNAHVLRINHQDGRAVSVEYIDLVTGEHHVQPGDVFYLGA
                     YTFQNVRLLLFSGITGDGQVGKYFINRSGPSVSAIFDDRYLNGYNGPSVQRQGLDDYN
                     GENNAEEKMQMSDEDFFVRGAFIGSPSQRFPLESYRSHPPEVPDWGREYKEYFTKYLN
                     RFITLQLLLEPLPYESSYIDLDPNYTDRFGVPAARVHRQAKQNEIRMSRYIFQKGKEI
                     LEAAGASQVWGRETPNATATMTHDLGGLRMGDDPSASVTNRYGQMWEVPNILVGGGAV
                     APTMSGHNPTETIWALAYWTADAVLQNKIDLEDSQAFS"
BASE COUNT          537 a         1043 c         1024 g          566 t
ORIGIN      
        1 cccggtgata tcagccagcc gacaatcaac tctgagtgaa accccagaat ctcctgcagc
       61 tcgccaccgt cggtctctgc aagggctaga gcgccgtcgt tgcccaggat cgccagaagg
      121 cggcgccgcg aggaccgacc atcgacgctg ccgagcggta cggcgaggcc tgagtccggg
      181 caatagtggt gtatcgatta tcaggtgcat cgagcgctac gagcgatgcg gtcagcgtcg
      241 cgatgggagc aggcaagata tcatcgacga tcgccttgct ggtcacgtcg tcccccgccc
      301 ggtcgcacca gccgtcctca acgcgatacg cctgcaccac agcgtcagcc agcgctcgtg
      361 cgcgctggtg ccactcgtcg tggttgacct ttctgccggc ggcgaccatc gcacccagta
      421 gtgccgcctg atcctcgagc caagcgttac catctgcccg aacgtagccg cgctgcgggt
      481 ccatgccgga gtcgagcgcg atggcgagca actcctggag gcgccccagt tccagaccgg
      541 gcagccactc cgacgcgaca aacagccagc gtagcgtctc ggcccgccag ctccaaccgc
      601 ccatggtcac cagctcgggc gctggtcgct gttgccggag cgtgagcatg tgcttccggc
      661 cacgctcgac tcgcgcccgc agtacctcca ggtcctcccg actgtactga tccagtcgct
      721 caacggggac tgcatggtgc acgacctggc gcagcgggct cggcccgatg ccgaagtgca
      781 gactcatggg ctggacttca tccacgttca agccgtccag caactcctta gaggtccatg
      841 tgtagtatgg gctgttactg cccacacagt ccacgttttc tcgcaatgcc gtcatgcaga
      901 aggtggccag cgcctgcgcg agctcggtga attcggggtg atcgagctca tgcccggcgc
      961 gcgcgtacac ggcggtgagc tgagcgttca gcgggacggg cttctcgaaa tgtggcacta
     1021 cccatcgctc atcacgcgag catcggtgga agccgcgatc taagtgatcg ttgatcccgc
     1081 cgtcggccat gtgccgcaac gttgcggtca cccagccgac aacctccttt gctgcgacag
     1141 acacgacgtg gtccagcgcg gtccagagca gttgcgggtg cggatgacgc ggcgactcgt
     1201 gcaggccgcc ccgaaccatg tccaggtcgg gctggagacc tgcccagaac gccgggaagc
     1261 ccgcaacgtc ccccgcgttc cgcgcatccg cttgcccgag cgatcgcgcc tcggcatcga
     1321 acgccgcggc gttctcggca taggcgtcgg ctgtcgccgt gattagcgac ccgagcgagg
     1381 gcagaaggtc gtccccctcg tactgcatgc cgggatagac gagcatcggc tccccatctg
     1441 gcgtcagcag gatcgtcaac ggaggtccgc tcgtgccggt gagcaccacg gctgcccacc
     1501 gccaccgggc agcgaggtcg ggccgctcgg tccagtcgac gaggaccggc accacccgcg
     1561 cctgcagcac gtcctgcagc tcgtcgtccc gctcgaggac atatgccaac cgttgggcgc
     1621 tgttcgacca ccgcggctcg gcaaaggcga ggatcggcac tccacgctgt cgtgcctcca
     1681 tgcgtccatc gctccagtcc cgccaacgca gcgaacggga gatatccggc atggtgtcag
     1741 gcggcccgga gacggcaggt gtggcgaggt tgaggggcat tccgttcata aagctcccat
     1801 tgcagatagg aaagatggca gaacgcggga gtggacgtgg tccacccccg cgatgcgaaa
     1861 ccgagtcttc aggcagccta gctgaaggct tgggaatcct ccaggtcgat cttgttctgg
     1921 agcacggcgt cggccgtcca gtaggccagt gcccagatcg tctccgtcgg gttgtgccca
     1981 gacatggtgg gtgccaccgc gccgccaccc acgaggatgt tgggcacctc ccacatctgg
     2041 ccatacctgt tggtcacgga tgcggaggga tcgtcgccca tgcgcagccc accgaggtca
     2101 tgtgtcatgg tcgcggtcgc gttcggtgtc tcccggcccc agacctggct ggcacccgcc
     2161 gcctcgagga tttccttccc cttctggaag atgtagcggg acatccggat ctcgttctgc
     2221 ttggcttggc gatgcacgcg cgcagccggc accccgaacc gatcggtgta gttcggatcg
     2281 aggtcgatgt agctgctctc gtaggggagc ggctccagca gcagctgcag agtgatgaac
     2341 cggttcaggt acttggtgaa gtactccttg tactcgcgac cccagtccgg cacctcaggt
     2401 gggtggctcc ggtagctctc caacgggaac cgctggctcg gtgagccgat gaacgcgccc
     2461 cggacgaaga aatcctcgtc cgacatctgc atcttctctt cggcgttgtt ctcgccgttg
     2521 tagtcgtcca gcccttggcg ctgcacggaa ggaccgttgt agccgttgag gtagcgatcg
     2581 tcgaagatcg ccgacaccga cgggcccgag cggttgatga agtacttgcc cacctgaccg
     2641 tccccggtga tgcccgagaa gagcagcagg cgcacgttct ggaacgtgta ggcgccgaga
     2701 tagaagacgt cgcccggctg cacgtgatgc tcgcccgtga cgaggtcgat gtactcgacg
     2761 ctcacggcac gtccgtcctg atggttgatg cgcagcacgt gggcattggt gcgcagctcg
     2821 aagttgccgg tttcctcggc ggtcggcagc aacgccgaga gcgtgctgct cttggagtgg
     2881 ttccagcagc catgccctgt gcagaaggtg cagtagctgc aggccgggcg tgagacgccg
     2941 tctggattgg tgtacggctc ggtggtgatc gccgtcggca catggaaggg attcagcccc
     3001 aactccaggg caccctcccg gaacgtcagg tccgtggcgt tgtcgcgcag tgggcggaac
     3061 gggtagtccc gctcgcgcgg cgcctcgaag gggttcccct cctccgggtt gaccgggcgg
     3121 atctcgccct ggatgttgcc agggaagccg ccgactccga tgtagtactc
//
LOCUS       DASYWL010000259         2589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_463623, whole genome shotgun sequence.
ACCESSION   DASYWL010000259 DASYWL010000000
VERSION     DASYWL010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463623"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VGR22_09365"
     CDS             <1..557
                     /locus_tag="VGR22_09365"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEV2128813.1"
                     /translation="TKYLSGHSNVIGGVIAGKATIIAAMRNRLAQLGGILSPQSGWVL
                     LNGVKTLALRMARHCGNGLAVAKAMAEHPAVETVHYPGLPSHRGHELAARMTGGRFSG
                     MVSVRLVEHGRARNIFLDALAIPLKAVSLGDVASLVWPFDEDGVIRLSVGIEDTEDLV
                     SDITQALDAVVAHLNTNAEASAQA"
     gene            complement(859..1551)
                     /gene="moaD"
                     /locus_tag="VGR22_09370"
     CDS             complement(859..1551)
                     /gene="moaD"
                     /locus_tag="VGR22_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HEV2128814.1"
                     /translation="MNITVRFFAMFREQLGTGERTIALPAGATAREGFDTALTDAPSL
                     QRLADTVMLMVNQEYADPDHVLQDGDELAVIPPVSGGDHAKRFAVTEQELDPRAVEAL
                     VAGPGEGAIVTFIGTVRNHARGRTVTLLEYEAYEAAAEKMLARVGAEACERWPDVTMA
                     ITHRTGALKPGEASVVIGCASPHRDAAYQASTFAISRIKEIVPIWKKEHYEDGSTWVG
                     SETEYQRLTGRL"
     gene            complement(1629..1856)
                     /locus_tag="VGR22_09375"
     CDS             complement(1629..1856)
                     /locus_tag="VGR22_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128815.1"
                     /translation="MSDHNHEFSVSDVEYNVITTLSNLLQSEEVLMKYATDAQEAGDS
                     ELEQMFHDLRRQNGEVAERLYRKLGQLASQS"
     gene            complement(2055..>2589)
                     /locus_tag="VGR22_09380"
     CDS             complement(2055..>2589)
                     /locus_tag="VGR22_09380"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEV2128816.1"
                     /translation="VSGAWGEADTPDQEALLGHVLSEGGWDRGRLGVTGGSYGGFMTN
                     WLLGHTDHFRTGVTDRSISNMASMYGTDDIALVSLDPELGAPWEHHERYCELSPIRAV
                     ANITAPLLIVHSEEDYRCPIEQAEQLFIALKRLGREVEFVRYKGEHHGLSRNGKPKNR
                     VDRLERTLGWFERHLAG"
BASE COUNT          391 a          863 c          863 g          472 t
ORIGIN      
        1 ccaccaagta cctctccggg cacagcaacg tcatcggggg tgtgatcgcg ggcaaggcca
       61 caatcatcgc ggcaatgcgt aaccggctgg cccagctcgg cgggatcctg tcaccacagt
      121 ccggctgggt cctgctcaac ggtgtcaaga cgctcgcact gcggatggca cggcactgcg
      181 ggaacggttt ggcggtggcg aaggccatgg ccgagcaccc ggcggtcgag acggtgcact
      241 atccagggct gccgtcgcac cggggtcacg aactcgccgc caggatgacc ggtggccggt
      301 tcagcggcat ggtgtcggtg cggctggtcg agcatgggcg cgcccgcaac atcttcctgg
      361 atgccctggc gattccgctg aaggcggtca gcctcgggga tgtggcctcg ctcgtctggc
      421 cctttgacga ggacggcgtg atccggctct ccgtcggcat cgaggataca gaggacctgg
      481 tttccgatat cacccaggca ctcgatgccg ttgtcgcaca cctgaacacg aacgccgagg
      541 cttcagccca ggcgtaggga tcacggcctg ctgcgatccc tcgagggcac gttcctgtag
      601 cctcggttcg agcctgtcct gagcagtgtc gtaaggatgc ccgagacccg tccacagggc
      661 ggcaacgcgg tgtggaacca gcatcggttg tcgatatgcg ggaatagcgt gctcatccat
      721 tgcgacgctc gcggctggca ccgcttctcg ctcagtggcg tgtcgagctt tgcgtggcaa
      781 cgcgataggg gccgatctgc gcagcagaga tcgcgctccg actcgggatg acggcaggga
      841 gggcaacctc atgggtctct acagccgacc ggtgaggcgc tggtactccg tctcgctgcc
      901 cacccaggtg gagccgtcct cgtagtgctc cttcttccag atcggcacga tctccttgat
      961 ccgcgagatc gcgaaggtgc tcgcctggta ggcggcatcc cggtggggcg acgcgcagcc
     1021 gatgacgacg ctcgcctcgc cgggcttcag cgcgccggtg cggtgcgtga tcgccatcgt
     1081 cacgtccggc caccgctcac acgcctcggc tcccacccgc gccagcatct tctccgccgc
     1141 ggcttcgtac gcctcgtact ccagcagcgt cacggtccgg ccgcgcgcgt ggttgcgcac
     1201 cgtgccgatg aaggtgacga tcgctccctc gccgggacca gcgaccagcg cctcgacggc
     1261 tcgcgggtcg agctcctgct cggtgacggc gaaacgcttc gcgtgatcgc cgccgctcac
     1321 cggcgggatg accgccagct cgtcgccatc ctgcagcacg tggtcggggt cggcgtactc
     1381 ctggttgacc atcagcatca ctgtgtcggc cagcctttgc agggacggag catcggtgag
     1441 cgcagtgtcg aagccctcgc gggcggtcgc gcccgccgga agggcgatcg tcctctcgcc
     1501 ggtgccgagc tgctcacgga acatggcaaa aaaccggacg gtgatgttca cggtacgggc
     1561 gcctctcgtg ctgttcgtgc tgttggatgc gggggcgacc cgccggtcgc ccccgcgctg
     1621 tcgtcagcct acgactgcga cgccagctga ccgagcttcc ggtagagccg ctcggcaacc
     1681 tccccattct ggcggcgcaa gtcgtggaac atctgttcca gttccgagtc gcccgcctcc
     1741 tgggcgtcgg tggcgtactt catgagcacc tcctcgctct ggagcaggtt gctcagcgtc
     1801 gtgatgacat tgtattcgac atcgctgacg gaaaactcgt ggttgtggtc gctcatgtgg
     1861 tgttcctccg atcgtctaat cgcgatggac ctggaggaac gttcgcaaga gccgggccag
     1921 acggtccgac ggtatccgat tgcatgctcc gcgatcgtct atgggagggt cgcgtgtccc
     1981 ctccctcgtc agggcgaggc gtgtcccggc ctgtacacga atggagatca ccgagtgatc
     2041 tcgcggcctt ggtactaccc cgccaggtgg cgttcgaacc agccgagggt gcgctccagg
     2101 cgatcgacgc ggttcttcgg tttgccgttg cgcgagagcc cgtggtgctc gcccttgtac
     2161 cgcacgaact cgacttcccg cccgagccgc ttgagcgcga tgaagagctg ctcggcctgc
     2221 tctatcgggc agcggtagtc ctcctcgctg tggacgatca gcagcggcgc ggtgatgttc
     2281 gcgaccgcgc ggatgggcga gagctcgcag tagcgctcgt ggtgctccca cggcgcgccc
     2341 agctccggat cgaggctgac cagcgcgatg tcgtccgtcc cgtacatgct cgccatgttc
     2401 gagatcgagc gatccgtgac gccggtccgg aaatgatcgg tgtgtccgag cagccagttg
     2461 gtcatgaagc cgccgtagga gccaccggtg acgccgaggc ggccgcggtc ccagccgccc
     2521 tcggagagaa catgccccag caacgcttcc tggtccgggg tgtcggcctc gccccaggcg
     2581 ccactgacc
//
LOCUS       DASYWL010000260         3534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_163154, whole genome shotgun sequence.
ACCESSION   DASYWL010000260 DASYWL010000000
VERSION     DASYWL010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163154"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..922)
                     /locus_tag="VGR22_09385"
     CDS             complement(68..922)
                     /locus_tag="VGR22_09385"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEV2128817.1"
                     /translation="MVSSLGVIAVGVISAVIGGPVFAAVMTAIGLGVYYETSRITPMI
                     GLGYPLGWPGYAVIFLGASVSLLSQEPWSFALFVSMAVILPAVMILRLPISNESFASW
                     IATAAMGAYVGIAVYAAIALRQEIGILEVDWANDLGAWFSLSDESRALGMAWTAIAII
                     ATWLGDTFALLVGRSMGRTPLIPHISPRKTLEGAVGGILGAVIGTVALTLLFGIPDIT
                     GAQAVIIGVVLSVVGVLGDLTESFIKRSGGVKDSGTLIPGHGGVFDRVDAMLPIFLVT
                     WVIVRILH"
     gene            complement(947..1669)
                     /locus_tag="VGR22_09390"
     CDS             complement(947..1669)
                     /locus_tag="VGR22_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEV2128818.1"
                     /translation="MPIDPTRIPRHVAIIMDGNGRWARQRGLARIEGHEAGTENIRRI
                     THAAGQLGIRYLTLWAFSAENWRRPREEVEGILNILGRAIETETEELHRQGAQLRHIG
                     DLGSLSSDLQRAIEGAIERTKHNDKLVLTLAFNYGGRQELLRAVQDIVASGVSPEHID
                     ESTVERHLYTHDLPDPDLIIRTSGEYRLSNFLCWQGAYSELHFSPTLWPDFSPSDLET
                     AVRDFAQRERRFGAISSSSTTG"
     gene            complement(1729..2286)
                     /gene="frr"
                     /locus_tag="VGR22_09395"
     CDS             complement(1729..2286)
                     /gene="frr"
                     /locus_tag="VGR22_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEV2128819.1"
                     /translation="MTAEILKDAESRMTKAMDALHHNLATIRTGRASPALLDRIHVDY
                     YGASTPLNQLAGISAPEPRLLVIQPWDRGSIGPIEKAIQTSDLGLTPNNDGQMIRISI
                     PALTEERRTQLVKVVHQTVEESKVAVRNIRRDAVSHLRKQLSDKTISEDDERRAIEAI
                     DAMSRKFVDRADEIGKEKEQEVLVV"
     gene            complement(2341..3078)
                     /gene="pyrH"
                     /locus_tag="VGR22_09400"
     CDS             complement(2341..3078)
                     /gene="pyrH"
                     /locus_tag="VGR22_09400"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEV2128820.1"
                     /translation="MPEPYKRILLKLSGEALMGDQQFGIDPLVAQKMAKEIRGVVGTG
                     AQIGIVIGGGNIWRGLSAASKGMDRATGDYMGMIATVLNALALQDALENEGVQTRVMT
                     AIEMRDVAEPYIRRRAIRHMEKERVVILAAGTGNPYFTTDTAAALRALELDADALLMA
                     KNRVDGVYSSDPRTDSNAQRYDHITHQQALEKNLRVMDASALALCRDNNLPIVVFDMT
                     VAGTICRIVEGESVGTLVSSTTNDTHR"
     gene            complement(3171..>3534)
                     /locus_tag="VGR22_09405"
     CDS             complement(3171..>3534)
                     /locus_tag="VGR22_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128821.1"
                     /translation="EVNCETDFVARTDDFRTLAHDIAMQVAATAPRYVSSDDIPEGDY
                     VGLESEFGSREDAIVQASLMDQVFIKNPKATIREHVKAHIGKLGENIIVSRFSRFELG
                     GESRFAAMTTQPTGTDEG"
BASE COUNT          575 a         1099 c         1211 g          649 t
ORIGIN      
        1 gagcagggcg atccgtgtgg tcatgtgata gtctccaggc agccagggtg gtgcatcagc
       61 agcgaaatca atgcaggatg cggacgatca cccaagtcac gaggaagatc ggcagcatgg
      121 cgtccacgcg atcgaagacc ccgccgtggc cggggatgag cgtgccgctg tccttcacgc
      181 caccactgcg cttgatgaac gactcggtga ggtcgccgag cacgccgacg accgagagca
      241 ccacgccgat gatgacggcc tgagccccgg tgatgtccgg aatcccgaac agcagggtga
      301 gggccaccgt gccgatgacg gcgccgagga tgccgccgac cgcaccttcc agcgtctttc
      361 tggggctgat gtgcggaatc agcggggtgc gacccatgct ccgcccgacc aggagcgcga
      421 aggtatcacc cagccaggtg gcgatgatgg cgattgccgt ccaggccatg ccgagcgcgc
      481 gggactcatc ggagagcgag aaccaggctc cgaggtcgtt ggcccagtcg acctcgagga
      541 taccgatctc ctgccggagc gcgatagcgg cgtagacggc gatgccgacg taggcgccca
      601 tcgcggccgt ggcgatccac gaggcgaagc tttcattgct gatcggcagg cgcaggatca
      661 tcaccgcagg caggatcacc gccattgata cgaacagcgc gaacgaccat ggctcctgcg
      721 agagcagcga gacggacgcg ccgagaaaga taacggcata gccgggccag ccaagcgggt
      781 accccagacc gatcatcggt gtgatccggg acgtttcata gtagacaccg agcccaatcg
      841 cggtcatcac cgccgcgaac acgggcccac cgataacggc tgatatcaca ccgaccgcga
      901 tcacaccgag tgaggaaacc aatctggtac gcagggcgcc ctcccgtcag ccagtcgtgg
      961 atgagctcga tatcgcgccg aatcgtcgct cccgctgcgc gaagtcccgc acggcggttt
     1021 cgagatcgct cgggctgaag tctggccaaa gggtggggga gaagtggagc tcggagtacg
     1081 cgccctgcca gcagaggaaa ttgctcagcc gatattcccc ggacgtccgg atgatcaggt
     1141 cgggatccgg caggtcatgg gtgtagaggt gacgctcgac cgtcgactcg tcgatatgct
     1201 ctggtgagac gccggaggcg acgatgtcct gcaccgcccg cagcagctcc tggcggccac
     1261 cgtagttgaa cgcaagggtg agcacgagct tgtcgttgtg cttcgtacgc tcgatcgctc
     1321 cctcgatcgc ccgctggagg tcgctggaga gcgaaccgag gtcgccgatg tgccggagtt
     1381 gcgctccctg gcggtggagc tcctcggtct cggtctcgat cgcccggccg aggatgttga
     1441 ggatgccttc gacctcctcg cgggggcgtc gccagttctc ggcggagaag gcccagagag
     1501 tcaggtagcg tattccgagc tgccctgcgg catgggtgat tcgccggata ttctccgtcc
     1561 ccgcctcgtg cccttcgatg cgcgccagcc ctcgctgccg tgcccagcgc ccattgccat
     1621 ccatgatgat ggcaacgtgc cgagggatgc gagtggggtc gatgggcatc gggtgctcga
     1681 gcggtgcggg cgcggatgac gcggggcgag ctgggtgtgc tgtcatccct agaccacgag
     1741 gacttcctgt tccttttcct tgccgatctc gtcggcccgg tcgacgaatt tcctgctcat
     1801 ggcgtcgatc gcctcgatgg ctcgccgctc gtcatcctcg gagatcgtct tgtcggacag
     1861 ctgctttcgc aggtggctga cggcatcccg ccggatgttg cggacggcga ccttgctctc
     1921 ctccacggtc tggtgaacga ccttgacgag ctgcgtgcgc cgctcctcgg tgagcgccgg
     1981 gatggagatg cggatcatct ggccgtcatt gttcggcgtc agcccgagat cggaggtctg
     2041 gatcgccttc tcgatcgggc cgatgctgcc gcggtcccac ggctggatca cgagcaggcg
     2101 cggctcgggg gcggagatgc ccgcgagttg gttgagcggc gtcgatgcgc catagtagtc
     2161 gacatggata cggtccagca gcgccggtga cgcccggccg gtgcggatcg tggcgaggtt
     2221 gtggtgcagc gcgtccatcg ccttggtcat ccgcgactcc gcgtccttca ggatttccgc
     2281 tgtcatgcag gtcctatcct ttcctggctc agggtcgaag cggggcacag aagcgatggc
     2341 tcaacggtgc gtgtcgttcg tggttgagga taccagagtc cccaccgatt cgccctcgac
     2401 gatgcggcag atggtaccgg cgacggtcat gtcgaagacg acgatcggca ggttgttgtc
     2461 tcggcagagc gccagcgcgg aggcgtccat cacgcggagg ttcttttcca gtgcctgctg
     2521 gtgcgtgatg tggtcgtatc gctgcgcatt gctgtcggtg cgcgggtcgg aggagtacac
     2581 gccatcgacg cggttcttgg ccatcagcag ggcatcggca tcgagctcca gcgcgcgcag
     2641 ggcggctgcg gtgtcggtcg tgaagtaggg attgccggtg ccggccgcga ggatgacgac
     2701 gcgctccttc tccatgtgac ggatcgcgcg gcgccggatg tacggctcgg cgacgtcccg
     2761 catctcgatc gccgtcatca cgcgcgtctg gaccccctcg ttctcgaggg catcctggag
     2821 cgccagcgcg ttcagcacgg tggcgatcat gcccatgtag tcgcccgtgg cgcgatccat
     2881 gcccttgctg gcggcggaga gtccgcgcca gatgttgccg ccgccgatca cgatgccgat
     2941 ctgcgcgccg gtaccgacga cgcccctgat ctctttcgcc atcttctggg ccacgagcgg
     3001 gtcgatgccg aactgctgat cgcccatcag ggcttcaccg ctgagtttga ggaggatgcg
     3061 cttgtacggc tctggcatga ggctggctcc gtggaggtgc agggcgctga atcggccgga
     3121 tatggaacga cgccgccttt ccggggaaag acggcgtcgc gttcggtgcc ttagccctcg
     3181 tcggtgccgg tgggctgcgt cgtcatcgcg gcgaagcggc tctcgccgcc gagctcgaag
     3241 cggctgaagc ggctgacgat gatgttctcg ccgagcttgc cgatgtgcgc cttgacatgc
     3301 tcccggatcg tggccttcgg gttcttgatg aacacctggt ccatcagcga ggcttgcacg
     3361 atggcgtcct cgcggctgcc gaactcggac tcgaggccga cgtagtcccc ctcggggatg
     3421 tcgtcgctcg acacgtagcg cggtgcagtg gccgccacct gcatggcgat gtcgtgcgcg
     3481 agtgtccgga aatcgtcggt gcgagcgacg aagtcggtct cacagttcac ctca
//
LOCUS       DASYWL010000261         4498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_125471, whole genome shotgun sequence.
ACCESSION   DASYWL010000261 DASYWL010000000
VERSION     DASYWL010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125471"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VGR22_09410"
     CDS             complement(<1..261)
                     /locus_tag="VGR22_09410"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV2128822.1"
                     /translation="MSTRNRPEGCCTVPNTPAPIAEGDRANLVMVFKALGDPTRLEIF
                     RLIAAQDAPICVCDVVDRFDLRQPTISHHLKVLRDAGLVTVSR"
     gene            complement(345..692)
                     /locus_tag="VGR22_09415"
     CDS             complement(345..692)
                     /locus_tag="VGR22_09415"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002016.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEV2128823.1"
                     /translation="MTLTSETCEACRADSPKVTEEEIRELKPQIPEWEMTEVRSVPHL
                     RRTFRFDGWMPAVRFVDALAELVEEQDHHPVIRLEWGKLTVSWWTHAIDGLHRNDFIM
                     AARTDEIYGKHSE"
     gene            1057..2127
                     /locus_tag="VGR22_09420"
     CDS             1057..2127
                     /locus_tag="VGR22_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV2128824.1"
                     /translation="MNENGMARKMVPLAGAIGAAVAVREVIGRSREADLRGEVALVTG
                     ASRGLGLALARELAAQGCRLVICARDEAELERAREDLEGQGAEVLAVPCDVGDKDDVD
                     RMVAQVRERFGRIDLLVCNAGVIQVGQVRSSELEDYRQAMDIMYWGVLYPILAVLPEM
                     RERQAGRIAIISSIGGKISVPRLVPYNASKFAAVGLAEGLRAELADEGITVTNIVPGL
                     MRTGSFLNAYFSGDEEGRTAQYKLFAPMSSLPLLTVSAESAARTYVRAIRRGQAEIIY
                     PPQYALVARLHGIAPATVARLLGVADRLIAPSGGGETTERGMAIDERVESRPWRWLTA
                     LGRRGAERLYQQPGPTDVPEPD"
     gene            complement(2199..2357)
                     /locus_tag="VGR22_09425"
                     /pseudo
     CDS             complement(2199..2357)
                     /locus_tag="VGR22_09425"
                     /inference="COORDINATES: protein motif:HMM:NF022101.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2385..2756)
                     /locus_tag="VGR22_09430"
     CDS             complement(2385..2756)
                     /locus_tag="VGR22_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128825.1"
                     /translation="MTEQTTTPPMKPSEESTKEQLMLAKKQGDAFQAAVKAMDQESGA
                     DITRAGEYEVGLVVEDAEGMYHLEGTGNLVWKEPENENAHIEVVVRDAADGRFIPGLT
                     VHVTVTDANGTEVGTHQQPML"
     gene            3448..4134
                     /locus_tag="VGR22_09435"
     CDS             3448..4134
                     /locus_tag="VGR22_09435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEV2128826.1"
                     /translation="MAVHSAYTLDALAQDDPALTGRQFFTSGQAETIEALAEQFWPTT
                     EDSPGGRDAGVMYYIDNALAGAYQEYKEVYRTGIDWLDDAAQREYGSVFRQLSSEDQT
                     ALITAIFEADDEEQAAPGLATPEQIEVEGIPLVDVTPVVVEPEPEGTGRTQVAGLQAP
                     TIASLPEFMSLVLTHTMEGLFSDPVYGGNRDFAGWRAVGYPGAHYLYTEEEQQSFEPL
                     NKPIQSLADL"
     gene            4165..>4498
                     /locus_tag="VGR22_09440"
     CDS             4165..>4498
                     /locus_tag="VGR22_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128827.1"
                     /translation="MAVQPKQDVVIIGYGAAAGPMSVELAKAGYSVVTLERGPYRGLE
                     EFSGGRAFDTLRYRVRDELNPKLPEVPLTFREDPNETAQPTNYIMANTVGGASVHWSG
                     QSWRYYVED"
BASE COUNT          764 a         1359 c         1531 g          844 t
ORIGIN      
        1 gcgagagacc gtgacgaggc ccgcgtcgcg cagcaccttg aggtggtgcg agatcgttgg
       61 ctgccgcagg tcgaatcgat cgaccacatc acagacacag atcggggcgt cctgcgcggc
      121 gatcagccga aagatctcga gccgcgtcgg gtccccgagc gccttgaaga ccatgaccag
      181 gttggcgcgg tcgccctcgg cgatcggtgc gggggtgttc gggacggtgc agcagccctc
      241 aggccggttt ctcgtactca tgtgaccatt gtacaggacg catagacgaa tgtcaataga
      301 tgcgccgacc ggttacgacg attcgccggg attctcagcc agggctactc gctgtgcttg
      361 ccgtagatct cgtcggttct ggcggccatg atgaagtcgt tgcggtggag gccgtcgatc
      421 gcgtgggtcc accaactgac cgtcagcttg ccccactcca agcggatgac ggggtggtgg
      481 tcctgttcct cgacgagctc ggcgagggcg tcgacgaacc gcaccgccgg catccagccg
      541 tcgaagcgga aggtccggcg caggtgcggc acgctccgca cctccgtcat ctcccactcg
      601 gggatctgcg gcttgagctc gcggatctcc tcctcggtca ctttcgggga gtcggcacgg
      661 caggcttcac acgtctcgga tgtcaacgtc atggtggctt cctttcaata gaggagatcg
      721 caggaggggt gcttcacgcg gtatcgatcc gctaggaccg gcacagtcga ttcgttcagc
      781 acaggctcgt gcgtcaggcc aaacgcgggt agacacgagg tctgccccta tgggctcaat
      841 cagtgatagg tgtaccctcc tgcacggtgc tccaatagag caacaggtag ctcgtggaac
      901 tgtgcagcaa gtggcgttcc gcggcgccaa ggcgtcgtca acgttacctg gaccggtacc
      961 cgggcctgag gcaggaccga tcccggtcgc acactggcat gacgcttgca tgcctccagt
     1021 caatccggaa gaacggtcac acgatgggag cgcagcatga acgagaacgg gatggcacgg
     1081 aagatggtgc cgctggcggg ggcgatcggg gcggctgtgg cggtgcgcga ggtgatcggt
     1141 cggagccggg aggccgatct gagaggcgag gtggcgcttg tgaccggggc ctcccgcggg
     1201 ctcgggctgg ccctcgcccg ggagctggcc gcacagggct gccggctggt gatctgtgcc
     1261 cgcgacgagg ccgagctgga gcgtgcgcgc gaggacctgg aaggacaggg cgccgaggtg
     1321 ctggcggtgc cgtgcgacgt cggcgacaag gacgatgtcg atcggatggt cgcgcaggta
     1381 cgtgagcgct tcgggcggat cgacctgctg gtctgcaacg cgggggtgat ccaggtcggg
     1441 caggtgcgga gctccgaact ggaggactac cggcaggcga tggacatcat gtactggggg
     1501 gtgctctacc cgattctcgc cgttctgccg gagatgcggg agcggcaggc ggggcggatc
     1561 gccatcatct cctcaatcgg cgggaagatc agtgtgccgc gcctggtgcc ctacaacgca
     1621 tccaagttcg ctgcggtcgg acttgcggaa ggcttgcggg cagagctggc cgacgagggg
     1681 atcaccgtga cgaacattgt gcccggcctc atgcgcaccg gctccttcct caacgcctac
     1741 ttcagcggcg acgaggaggg gcgcacggca cagtacaagc tcttcgcgcc gatgtcgagc
     1801 ctgccgctcc ttactgtgag cgcggagtcg gcggcgcgga cgtacgtccg ggcaatccgt
     1861 cgtggccagg cggagatcat ctatccgccc cagtacgcac tggtggcgcg tcttcacggg
     1921 atcgccccgg cgacggtagc ccgactgctc ggtgtcgctg accgcctgat cgcgcccagc
     1981 ggcgggggag agacgaccga gcgcgggatg gcgatcgacg agcgggtgga gtcgaggccg
     2041 tggcggtggc tgacggcgct cgggcgccgt ggcgcagagc ggctctacca gcagcccggc
     2101 ccgacggacg tgcccgagcc ggactgacga catacccagc cccggcccgg ttacagggcg
     2161 gggtgctgtc gcatcgtggc catgttgagg gtctgcagct acgcggcgcg cttctggccc
     2221 ggttcgatgt gccgcatgaa ctcgacctcg acatcctcgg cgaagcgctt gccgttctcg
     2281 tggtcgtggt gcatgaaggt cggcgcatcg atcttcacgc cgatggtgta gttgccctcg
     2341 cccggcacct cccaattgcg gccgtagtgg tagagccagg gatgctagag catcggctgc
     2401 tggtgcgtac cgacctcggt gccgttcgcg tcggtgacgg tgacgtggac ggtcaatccg
     2461 gggatgaagc gcccgtcggc ggcgtcgcgc acgacgacct cgatgtgggc attctcgttc
     2521 tcgggctcct tccagacgag gttgcccgtg ccttcgaggt ggtacatgcc ctcggcgtcc
     2581 tcgaccacga ggccgacctc gtactcaccg gcccgggtga tgtcggcacc ggactcctgg
     2641 tccatcgcct tgacggcggc ctggaacgcg tcgccctgtt tcttcgcaag catcagctgc
     2701 tccttcgtgg attcctcact gggcttcatc ggtggtgtgg tggtctgttc ggtcatcatg
     2761 tctcctttgc tctctagagc cggatagcct gtcgtcatgc gatctctgct tgggtcgctg
     2821 cccacaaaga gtgccagccg atcccgttgg ttgccggtca gctcatagtg tcggagactc
     2881 atgcctcaac ggatcattcg caaggcccag catcatctgt gccgctactt ctgtgatgtt
     2941 ggagaaccct cctcgagtgg gaaggtacat ctgtttagcc gtcacgtatg gtaagaattt
     3001 tttcgtgtct cgaatcagtg ctagcgtacc gagccgatgt tcgtgggcaa ggggttgact
     3061 agctggggaa gttggagttt cattgtggta cgactgcaca gaggccagaa cgcgcgcccc
     3121 caatgtgccg gacccgccgg gaaactcgac cagcattccc cgagtatttc attgaagctt
     3181 ccggagacat gctggccttt atgtctgttc agccgccact gagtcctgtt ccagtctctt
     3241 gagtgttttg agtcgtatgg aagcaaggga ggtgaacctg ggagaggtcg cggtagtgct
     3301 gatctgagat tacgcgctgc tcacatatgt cagttgccac cagtaacgag gtgcatcatg
     3361 gaccaacgaa gagaaccccg aggcgcccgc agcttctcag tggatcgccg gtcgttcctg
     3421 aaggtatcag ctggttctat ggccgcgatg gcggtccatt cggcctacac gctcgatgcg
     3481 cttgctcaag acgatcccgc gctgaccggc cggcagttct ttaccagcgg gcaggcggaa
     3541 acgatcgagg cccttgccga gcagttctgg ccgaccaccg aggacagccc cggtgggcgc
     3601 gatgccggtg tcatgtacta catcgataac gccctggctg gcgcctatca ggagtacaag
     3661 gaggtctacc gcaccgggat cgactggctg gacgacgccg cgcagcggga gtacggctcg
     3721 gtattccggc agctctcctc ggaggatcag accgccctca tcacggcgat cttcgaggcc
     3781 gatgatgagg agcaagccgc tcctgggcta gctacccccg aacagatcga ggtcgagggc
     3841 atcccgcttg tggatgtgac ccctgtcgtg gtcgagcccg aaccggaggg cacggggcgg
     3901 acccaggtgg cggggctcca ggcaccgacc atcgcctcgc tgccagagtt catgagcttg
     3961 gtcctcaccc acacgatgga ggggctcttc tcggatcccg tctacggtgg aaatcgtgac
     4021 ttcgccgggt ggcgggctgt tgggtacccg ggcgcgcact atctctacac cgaggaggag
     4081 cagcagtcgt tcgagccgct gaacaagccg atccagagcc tcgcggacct ctaggacgcg
     4141 caacgcatca gggagcataa aaccatggca gttcaaccta aacaggatgt cgtcatcatc
     4201 ggttatggcg cggcggccgg tccgatgtcc gtggagctcg ccaaggccgg ttattcggtc
     4261 gtgacactcg agcgaggccc atatcgcggt ctggaggagt tctcgggcgg ccgggccttc
     4321 gataccctgc gctatcgggt ccgcgatgag ctgaacccca agctgccgga ggtgccgctc
     4381 acgttccgcg aagacccaaa cgagacggcg cagccgacca actacatcat ggcgaacacg
     4441 gtcgggggcg cgtcggtgca ctggtcgggt cagtcctggc gctactacgt tgaggatt
//
LOCUS       DASYWL010000262         4806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_219875, whole genome shotgun sequence.
ACCESSION   DASYWL010000262 DASYWL010000000
VERSION     DASYWL010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219875"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VGR22_09445"
     CDS             <1..238
                     /locus_tag="VGR22_09445"
                     /inference="COORDINATES: protein motif:HMM:NF017880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HEV2128828.1"
                     /translation="CTSGLEPLRNVGNMFYTGDTRDYLVLVIDVTKITSEVRYDDPER
                     IYPHIYGPLNAETVIGLLDVERSADGTFIKFTER"
     gene            297..2507
                     /locus_tag="VGR22_09450"
     CDS             297..2507
                     /locus_tag="VGR22_09450"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEV2128829.1"
                     /translation="MGVRHKWLVAVFWIALVLVSVPFALNAQEPLEVGGFSSDETEAS
                     RTIRAVQDRLGYSPSTLIVVYESETLAADDPAFLSQVETSLEGIRELPFVESVVTPEL
                     DASLVSESREIAYALIGVDLPPEEAQRDVPAFEATLVPQGDIDFLVAGGPAFYADIET
                     ASQRDLRRAEIIALPVALIALLVVFGTVVSALVPLVTGSAGVAVVLMAIYHLATVVDL
                     SIFVLNLATMLGLGLAVDYALFVTSRYREELRKNGGDVAVAIERALETAGRAVFFSGL
                     SVLVGLSGLIIFPMMFLRSVGIAGVVVVAISTLTSLTLLPAVLAIIGTRIESLSIGRL
                     GGVSVGQGGTGRWYGIAHTVMRRPVIVALLTFVLLATLGLPFRHANISSPDATILPQD
                     LPSRQGFDLLSEEFSGGEISPFVILLNFDESVEERERVAVVARLETLLAEDERIERAK
                     GPTTFLEGLGALSPQTRFDLRQQAEQLGTETQLDRFWAEDSAVILAYPVEPANQPENK
                     ELLEQLRSIPDTERVQVLVGGGTAGIVDVVDEIYERFPVAAAIVVFTTYVILLVLFRS
                     VVLPIKAIFLNVMSILASYGALVFVFQEGHFHALLNFTPQGFVEASLPVIMFCVLFGL
                     SMDYEVFLLTRVREEWERTGNNREGVALGLDRSGRIITSAALIVVVVTGSFVSADVVL
                     VKALGLGIAIAVAVDATVVRGLLVPATMVLLGNVNWWMPDWLERALPGTAMREG"
     gene            2734..3102
                     /locus_tag="VGR22_09455"
     CDS             2734..3102
                     /locus_tag="VGR22_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128830.1"
                     /translation="MASYDVAINRAGRLARLGGWLQALRGMLMVVHTAEEDDTFVWRR
                     GRDVVRLRREGEGWLVSYQTGGRLLGPQRSVYEARHRIAKHAAWDMMARVINASHDEN
                     EGVKAASMAAKWMRRISSAG"
     gene            3111..3965
                     /locus_tag="VGR22_09460"
     CDS             3111..3965
                     /locus_tag="VGR22_09460"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEV2128831.1"
                     /translation="MPELPEVETIRRIVEAHLTGHSLRSFEVSLPKVLRQSAISNPET
                     LVGRTVISASRRGKVLDIAFDGDLHLLVHFKLAGQLALDLPNGERLVAGHPVPKPEGP
                     YPHKATHARLGFDDGRVAWYSDVRQFGWMNILPAADVWSTLDALALGPEATAPIDVDR
                     LAGLFERRSVPVKALLLDQHVLAGLGNIYVDEVLFRVGVHPATPAKAIPREAIQRIAA
                     NIPPVLAEGIRQGGATIIHGKAYPENDFPAVHGREGEACTRCGDTILKTRVTGRGTYL
                     CPVCQPTP"
     gene            complement(4077..>4806)
                     /locus_tag="VGR22_09465"
     CDS             complement(4077..>4806)
                     /locus_tag="VGR22_09465"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HEV2128832.1"
                     /translation="TYIDDDGFVRVDGIGGWDDQVVVGQRVVIIGRHGPVKGVVGKKA
                     THLLKSTDRKKVEKLSDLWIDIGATNREDAAARIEIGDPAVIDVAPLQLTDDLLVSRG
                     LDNRIGAFVVMETLREVGTDTPHRITALAASQEEITMAGAINAAHGLQPDVVIALDVT
                     HATDYPGADKKADNRVSLGGGPVLTRGAAVNDSVHRALREAAGEVGVTVGIQSAGRST
                     GTDADGFRRSGIASAIGLVSIPNR"
BASE COUNT          783 a         1514 c         1582 g          927 t
ORIGIN      
        1 ctgtaccagt gggctcgaac cgctccgcaa cgtcggcaac atgttctaca cgggtgacac
       61 tcgcgactac ctcgtactgg tgatcgacgt gacgaaaatc acctccgagg tgcggtatga
      121 cgatcccgag cgaatctatc cgcatatcta tggcccgctc aacgccgaaa ccgtgattgg
      181 cctgctggac gttgagcgga gtgccgacgg caccttcatc aaattcactg agcggtaggg
      241 ccggaaggtg caggggccga gaatgagtac cgctcgtgtt tagacggttc gcatgcatgg
      301 gcgttcggca caagtggctg gtagcagtct tctggatcgc gctcgtgctc gtgtcggtgc
      361 cgttcgccct caacgcccag gagccgctcg aggttggcgg attctcatct gacgagaccg
      421 aggccagccg aacgatccgt gccgtgcaag accggttggg ctacagcccg tccacgctca
      481 tcgtcgtcta tgagagcgag acgttggcgg cggacgatcc agcgtttctg tcgcaggtgg
      541 aaacctcgct cgagggcatc cgcgagctgc cattcgtcga atcggtggtc acgccggaac
      601 ttgacgcttc gctggtctcc gagagcaggg aaatcgccta cgcactgatt ggggtcgacc
      661 ttccgcctga agaggcccag cgcgatgtgc ctgcctttga agcgactctc gtcccacagg
      721 gcgatatcga cttcctggtg gccggcgggc ctgccttcta cgctgatatc gagacagcca
      781 gccagcgcga cctgcgccgc gcggagatca tcgccttgcc ggtcgcgctg atcgcgttgc
      841 tggtggtctt cggaaccgtc gtgtccgccc tcgtgccgct ggtcaccggt tcggcaggcg
      901 tggcggtggt gctgatggcg atctaccacc tcgccacggt cgtcgacctc agcatcttcg
      961 tcctcaacct cgcaacgatg ctggggctcg ggctcgccgt cgattacgcc ctcttcgtga
     1021 ccagtcgata ccgagaggag ctccggaaga acggcggcga tgtcgcggtg gcgatcgagc
     1081 gcgcgcttga gacggccggc cgggcggtct tcttctcagg cctctccgtg cttgtcgggc
     1141 tttccgggct gatcatcttc ccgatgatgt tcctgcgatc ggtcggcatc gccggcgtcg
     1201 tggtcgtcgc gatctcgacg ctgacgtcac tgacgctgct gccggcggtg cttgccatca
     1261 tcggcacgcg catcgagtcg ctgtcgatcg gtcgcctcgg gggtgtgtca gtcgggcagg
     1321 gcggaaccgg acgctggtac ggtattgccc acacggtgat gcggcgtccg gtgatcgtgg
     1381 cgctactgac attcgtattg ctcgccacgc tcggcctgcc attccgtcat gccaacatct
     1441 cttcacccga tgcaacgatc ctcccccagg acctgccgag ccgccaggga tttgacctgc
     1501 tgagtgagga gttctcgggt ggtgaaatct caccgtttgt catcttgctg aacttcgatg
     1561 agtcggttga agagcgagag cgcgtggccg tggtcgcgag gctcgaaacg ctgctagcgg
     1621 aagacgagcg gatcgagcgg gccaagggac cgaccacgtt cctggaggga ctgggcgccc
     1681 tgtcgccgca gacgaggttc gatctgcgac agcaggcgga gcaactcggc accgaaaccc
     1741 agctggatcg attttgggcc gaagacagcg ccgtgatctt ggcgtatccc gtggagccgg
     1801 cgaaccagcc ggagaacaag gagctgcttg aacagctgcg ctctatcccg gatacggagc
     1861 gcgtgcaagt gctcgtggga ggcggcacgg cggggatcgt cgatgtggtc gacgagatct
     1921 acgagcgctt tccggtggcg gcagccatcg tcgtcttcac gacatacgtc atcctgttgg
     1981 tgctcttccg gtcggtggtg ctgccaatca aggcgatctt cctcaacgtg atgtcgatcc
     2041 tggcttcgta tggcgcgctc gtcttcgtgt tccaggaagg gcatttccac gcgttgctca
     2101 acttcacgcc gcaggggttc gtcgaggctt cgctgcccgt catcatgttt tgtgtgctct
     2161 tcgggctctc gatggactac gaggtctttc tcctcacgcg cgtacgcgag gagtgggagc
     2221 gaaccggcaa caaccgggaa ggggtcgcgc tgggcctgga tcgcagcggt cggatcatca
     2281 ccagcgcggc gctgatcgtc gtggtggtga caggctcgtt cgtgtcggcc gatgtcgtcc
     2341 tcgtgaaggc gctcgggctg gggatcgcga tcgccgtggc agtggatgcg accgtcgttc
     2401 gcggcctcct ggtacctgca accatggtat tgctcggcaa cgtgaactgg tggatgcccg
     2461 attggctcga gcgtgcgctt cccggaacgg cgatgcgcga ggggtaggac ggccccgggc
     2521 atgaagcgtg gagtacactg aggatggctc gcgggatgga gggtcccgcg catcggctca
     2581 ggctcccgtc aggacccgcg aagggtgcgc tcgtgcgcgc cgcgaaccct gagcgcgacg
     2641 ccccgcaact cgcatgtctt gccaaacgtc agtctgctgc tgcggtggtg gcccctctcg
     2701 atcctgcaac ctgacacctg gtgggggaaa atcttggctt cttacgatgt cgcaatcaac
     2761 cgtgccggac gccttgcgcg gctcggcggt tggctccagg cgttgcgagg catgctcatg
     2821 gtcgtgcaca cggcggaaga ggacgatacg ttcgtttggc gtcgcggacg ggacgtggta
     2881 cgcctccgtc gggaaggaga gggctggctg gtgtcgtacc agaccggagg ccgcctgctc
     2941 ggtccgcagc gaagcgtcta cgaggcccgg catcggatcg ccaagcatgc ggcgtgggac
     3001 atgatggcgc gtgtcatcaa cgcttcccac gacgagaacg aaggggtcaa ggcggccagc
     3061 atggccgcca agtggatgcg gcgcatcagc tccgccggct agggcgggcc gtgccagagc
     3121 tcccagaagt cgagaccatc cgccggatcg tcgaagctca tctcaccggt cactcgctcc
     3181 gctcgttcga ggtttcgctg ccgaaggtgc tccgacaatc ggcgatctcc aacccagaaa
     3241 cactggtcgg ccgcactgtc atcagcgcct cgcgccgggg caaggtgctg gacatcgcct
     3301 tcgacgggga tctgcacctg ttggtgcact tcaagctggc cgggcagctt gcgctcgacc
     3361 tgcccaacgg cgaacggctc gtcgccggac atcccgtgcc gaaaccagag gggccctatc
     3421 cgcacaaggc aacgcatgcg aggcttggct tcgacgacgg tcgcgtagca tggtacagcg
     3481 acgtccgcca gtttggctgg atgaatatcc tgccggcagc cgacgtttgg tcgaccctgg
     3541 atgcgctggc cctcggaccc gaagcgaccg ctccgattga tgtcgaccgc ctcgctgggc
     3601 tgttcgagcg gcggtcggtg ccggtcaagg cgctgctgct cgaccagcac gtgctcgccg
     3661 ggctcggcaa catctacgtg gatgaggtgc tgttccgcgt cggggtccat ccggccaccc
     3721 cggcaaaggc gattcctcgc gaggccatcc agcgcatcgc ggccaacatc ccgccggtgc
     3781 tggccgaggg cattcggcag ggcggggcga caatcattca cggcaaggcg taccccgaaa
     3841 atgacttccc ggctgtccat ggacgggaag gagaggcgtg cactcgctgt ggcgatacca
     3901 tcttgaagac acgcgtcacc gggcgtggca cctacctttg cccggtctgc cagccaacgc
     3961 cttgagcctc agtcccgaaa atcaacgccc tcaggcaggc tccgtatgaa cgcagcgata
     4021 gtcgcgatgg cgccctcgac gtcgcgcagg ctgaccactt cgtttgggct atgcatctag
     4081 cggttcggaa tcgagacgag cccgattgcg ctcgcgatac ccgaccggcg gaacccatcc
     4141 gcgtccgttc cggtgctcct tccggcgctc tggatgccca cggtcacgcc cacctcgcca
     4201 gcagcttcgc ggagggcgcg gtggaccgaa tcgttgaccg cggctccgcg cgtgagcacc
     4261 ggtcccccac cgagtgagac gcggttgtct gctttcttgt cggcgccggg gtagtctgta
     4321 gcgtgggtga cgtcgagcgc aatcacgacg tccggttgca acccgtgtgc cgcgttgatc
     4381 gcgccggcca tcgtgatctc ctcctggctt gcggcaagcg cggtgatgcg gtgcggagtg
     4441 tcggttccga cctcgcgcag tgtctccatc acgacgaagg cgccgatacg gttatcgaga
     4501 ccgcgagata cgagcaggtc atccgtgagc tgcagtggcg cgacatcgat cacggctggg
     4561 tcgccgatct cgatccgcgc cgcagcatcc tcccggttcg ttgcaccgat atcgatccag
     4621 agatcggaga gcttctcgac cttcttccga tcggtggact tgagcaagtg cgtggccttc
     4681 tttccgacca cgcccttgac cggaccgtgt cggccgatga tgacgacgcg ctgcccgacg
     4741 acgacctggt cgtcccagcc gccgatccca tcgaccctga caaagccatc gtcgtcgatg
     4801 taggtg
//
LOCUS       DASYWL010000263        14315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_407569, whole genome shotgun sequence.
ACCESSION   DASYWL010000263 DASYWL010000000
VERSION     DASYWL010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 14315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14315
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407569"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VGR22_09470"
     CDS             complement(<1..221)
                     /locus_tag="VGR22_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2128833.1"
                     /translation="MSAFDVSIRQLYKRFGNLVAVDYIDLEVPSGAFVTFLGPSGCGK
                     TTTLRMIGGFEHPDSGQILIKGEEMGNRPP"
     gene            complement(391..1503)
                     /locus_tag="VGR22_09475"
     CDS             complement(391..1503)
                     /locus_tag="VGR22_09475"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV2128834.1"
                     /translation="MGKKDMQPKGLNRRSLIKGAAGAAAGGSALSTAIAQASTGNPGF
                     NINLRQDNGELVIYNWYQPWIAEVVPIFEEEAGINVTQLGTYSSNDEWWARLNAGENF
                     DFFIPSTDWVQRAMAANLLHPLDEGQIPNIDNLYEEFQENEIYQQDGNTYAVPFSRVY
                     YSLTYNTEEFPEAPTSWGVTWDAQYDGRITMQDQAYARVGTTALYIGDDPIQPTEWDR
                     IRELLLEQKELVFKYWTDYQNGMEMFINEEALVGQLTAGRTRMGAAEGAPIDWTVPEE
                     GVLTFIDTFAIPSTSENPENGHKFINFLHRADIMAMEMRMMYYDTLNRAAREELEPEL
                     AEQFAVPEESILVLTTDLDPQVRQRIDELWTEVKLS"
     gene            1718..2272
                     /locus_tag="VGR22_09480"
     CDS             1718..2272
                     /locus_tag="VGR22_09480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2128835.1"
                     /translation="MHPQITFRPLVDADLPAMKRWLEDPDVAWWYQAGSTAVEALREH
                     YRDEIAGTSRTRAFIIGLDGRDAGYIQCYAIDDEPKYAQQIKVDPGAVGIDLFIGESW
                     ARNRGYGRQVLRAFLAQIVFVKMGAEVAIIAPEPGNTRAVRSYERAGFVGWKIVYVVD
                     KDAPQNTGQEYIMRLTREAFESGE"
     gene            complement(2425..3264)
                     /locus_tag="VGR22_09485"
     CDS             complement(2425..3264)
                     /locus_tag="VGR22_09485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2128836.1"
                     /translation="MTDGTGPPSTYPPSRTISRSAWGIIALGLVALAALTLPTISLLA
                     RTLDANPSIDDERTRETLIAALRLSLMTSTIAIVVIVAIGTPVAWMLARYRLPAHRLL
                     DSLIDLPIILPPAVAGIALLSAFGRRGLLGDELDALGISVGFSTAAVVIAQVFVGAPF
                     YIRTARTGFGRVDVGMEEAAADLGAAPSRVFWRVVLPMTRSSLAAGAALAWARALGEF
                     GATILFAGNRQGVTQTMPLAIYERFQAGDLPTALLLSVVLLAGSAMLLVVVRLLGEPG
                     TPR"
     gene            complement(3291..4178)
                     /gene="modA"
                     /locus_tag="VGR22_09490"
     CDS             complement(3291..4178)
                     /gene="modA"
                     /locus_tag="VGR22_09490"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV2128837.1"
                     /translation="MHHRPGALTIALVLVMLMTSLASAPSVGAIELTCPPPATPPAAG
                     TPSKREAQNAAFPDDEIELTVFAAASLTDAFVDIADEIEPRHPNVSISVETAGSQTLV
                     TQLGEGAEADVLATASLATMRQAQESGLVNGEPVLFAANRLVIVTPPDNPAGIEGIDD
                     LAGDDIRLVIAAEDVPAGRYARQAICDWAGADDEAVAAIGANVVSEETDVRSALTRVQ
                     LDEADAGIVYASDAAAAELAGGEVKVVEFPSDVPTEATYPIAPTVGGNTEAARAFIKF
                     VLSDAGQRILADYGFAPVP"
     gene            complement(4334..5509)
                     /locus_tag="VGR22_09495"
     CDS             complement(4334..5509)
                     /locus_tag="VGR22_09495"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HEV2128838.1"
                     /translation="MTTALTEIWFRQRPVVLSPDLDPASPLPISPIEFHRRLPDYQPT
                     PIHDLHDLAGEIKVEQVLVKDETSRLGLPAFKMLGASWAIYRALLARWPDIDHNWATI
                     DDLRAQVQAREPISFAAATDGNHGRAVARMAAMLGCEATIVVPSSMVPARIEAIEGEG
                     ATVEIVEGTYGDAVARSAELASDSCLVISDTAWLGYDLIPRWVIEGYSTMFHELQEQC
                     RLTPSHTLVPMGVGALAAAAIVAARHRMPPATVIGVEPDGAPCVLKSLQRGAPVAIPG
                     PHESIMSGLNCDQPSPIAWPVLAAGLTAAISIPDDAAKEAMRILARKGVVAGETGAAS
                     LAGLLAIASASDATNLRSRLHLDKRASVLILATEGATDPEAWEKITRRSLQKTLAKR"
     gene            complement(5506..6090)
                     /locus_tag="VGR22_09500"
     CDS             complement(5506..6090)
                     /locus_tag="VGR22_09500"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV2128839.1"
                     /translation="MTDRPRSASRPRRALRLGPAEHSLLGLLAGAGEEGIHGYDLARQ
                     FSHGALGEIIRLEPGMLYHHLKKLDRYRLITTEIERQVDRPDRRIHTLTLDGREALDQ
                     WLQEPVHATREIRLDFLLKLFFTRRLAPARLAHLIGQQRAVLTDLLRSLTAQMEVGTA
                     APDAERRMVLRLRLAQTEAALEWLDSLDTTENNA"
     gene            complement(6117..7691)
                     /locus_tag="VGR22_09505"
     CDS             complement(6117..7691)
                     /locus_tag="VGR22_09505"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEV2128840.1"
                     /translation="MPIGTVASICSHRPPFGLEDPISTAFGLIAIIVLVLLNGFFVAT
                     EFALVSVRRTRIQQLSSEGDSRATRVLDRIDHLDTYIAATQLGITMASLALGWIGEPA
                     LAHVLHPLIERLPFEVSSTVMHTISFVVAFSIVTALHIVLGELAPKSLALQRPEETSL
                     LVGEPIHWFLIVFRPVINALNWVGNQVVRMLGFEPAQGHELVQSAEELMIALDASREA
                     GLVTQTAHDILDRAFSFTDLQAREVMVPRTEVTALPADASLDDVVRMTVDTSYTRLPV
                     YEGDSDNIIGIIKAKRMLPLFLQWAAQRAPQTALPNGSAPHIATVESSDRAANGTADI
                     TGLTPHAVTRDPNGTTVADTFRLSDYMVEPTLVPETLAVSEVLTRMQETHVQMAVVID
                     EYGGTAGIVTLQDIVNRLLGRLTEEEDEASGSEGLAADGTIHLDGLTSLSELKDEYGI
                     DLADDGLDVDTIGGYVFFRLGRAAQIDDEVQTSSGERIVVEALDGLRVARVRLLGRAA
                     LLEEDQGARDTLRIPA"
     gene            7867..8481
                     /locus_tag="VGR22_09510"
     CDS             7867..8481
                     /locus_tag="VGR22_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2128841.1"
                     /translation="MSGTGRPASERLDHLAALLSSQVRAEVLVHAVTRADTGHSLTEL
                     AHGLGLAISSVQHEAYKLQDLGLFSLKREGASRRYSLRLEEPVVQALRELVVATVGAP
                     RVVEMALTDLPGLAAGSLLGAIPPRQGETALVLIGDLELETVEQAHWRASWLLDAPAE
                     AVELAFFRPADWLARRDAGHPLVRRLLDLPVRAAFGDVRVDEQP"
     gene            8728..9918
                     /locus_tag="VGR22_09515"
     CDS             8728..9918
                     /locus_tag="VGR22_09515"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: protein motif:HMM:NF010675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HEV2128842.1"
                     /translation="MTEQQAPPTMVIGQSGGATAVINASLAGALEVGLASDEVGRIFG
                     MRHGIEGLLNSDLVDLGNIDAGRRDVLKRTPSAALGTGRYKLKDDDLDPILDICAREN
                     IRWILYIGGNDSADTSSRLARHAEARGQELSVIAIPKTVDNDLPETHHCPGYGSVARY
                     LANTARDAIYDTFASPQLYAVKFIEVMGRDAGWVTAACALATHGDEADLSPLLLLPER
                     PVEDVDAIIALIRADIARRGWSVVVVPETLQDREGRHLGGDEPEFVDAFGHPYYPSPG
                     AALSRVLTSAGLRARYDKPGTAARMSNAMASPVDLAEAEALGAEAARRAIVGETARMT
                     ALRETDEGRMEIVAVPVEQIANRVRLLDDAFIGKDGHSTTQAFLDYALPLLGPDPFPP
                     YARI"
     gene            10327..10755
                     /locus_tag="VGR22_09520"
     CDS             10327..10755
                     /locus_tag="VGR22_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128843.1"
                     /translation="MSKRDDQKRLPRYRNPDAGLTDLIQQSHALSRPLAGIIRYGEAD
                     EETGEVDEREIDVGADVLSHDGDKLGEVVNVMDDYLVVEQGFFNPHDVYIPISAIAWH
                     GDDCLYLRMTRDEFEASDWTIEPAPKADHLRNPHDTGEER"
     gene            10780..13032
                     /locus_tag="VGR22_09525"
     CDS             10780..13032
                     /locus_tag="VGR22_09525"
                     /inference="COORDINATES: protein motif:HMM:NF025011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HEV2128844.1"
                     /translation="MERGVVRNLLGRLNARPPGDILRIAERWQVPLSGTDTRRHVGAL
                     YRTMTDVRAARTFFDNLSDDIRAVVQTMALADGRLYTIAELARSIDRPEKAARDAAVW
                     LFYAGVLAREGDRQELPVGATPRLFLPRELEQVFTRAIDEIEVGDMRQESLRSLLALL
                     DDSDVEEAASDWGIQVIPGLRSRDDLAEDLLRLMDEPDRIRRVAGALGRDAKSLWEVV
                     REASEADGGMPYHDALVAAGLLGQDAQRPRNAREAGHILETLQEAEGKLLVWHSYDNE
                     GNRWLFVPQEIRHPGQRVRTLELKPLTPVPEKEVTPSEPHHPHAVAWDLLTMLRELIS
                     HRSPGWKPGEPLARGWQRQIGGHLWLSDDGVPEEGYPGFLLSLAHRVGLVEPGEKPAR
                     TGAEKGAFRPKVTDAIRGWRGQSFSSQTGQLRAAWLDHEDWIEGRERDEVEIWGASWP
                     DVRRRLLEVLGSLEPGTWYRQRELALHLAQQHPGLLGSTFTVASARGGDADEGREGRI
                     AKVAQVIGIELESAFHWFGLVEIGVTGTGTKRMRTARLTEAGELARERAEVVPGDDAA
                     LPGAPELDADAPVITLDEAGEVTLNRPSPLHIWSLSAFSDPVDLTPMARYVLTKASLG
                     RALGAGFDLDQVVQYLERQSGEPVPEPLMQHLREWTVGYRRVRLRRLAAVQPDDTELL
                     DDLARVLEEAGFEIADRLPEGDGLRVWLPETGDDAATAEDALQRALRANGFVGQWPRR
                     AEPLPRRPRD"
     gene            13365..>14315
                     /gene="gltX"
                     /locus_tag="VGR22_09530"
     CDS             13365..>14315
                     /gene="gltX"
                     /locus_tag="VGR22_09530"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEV2128845.1"
                     /translation="MTDEQKIIATEDRPRAIPVGLDEVRVRFAPSPTGSLHIGGAKLV
                     MYNVLFAHGQAKREGKKGTFVLRIEDTDQKRFVEGAAENLMDELRWLGLEWDEGPDVG
                     GPYAPYVQSERQHHYREAAERLVEEGHAYRCFCSPERLAQVREDLQKQGLPPGYDRHC
                     RNLPQEEIQAKLDAGEGYVVRFKMPLEGETRFTDLLRGEIVYENEKLEDLVILKSDGF
                     PTYHLAVVVDDHEMAITHVTRGPEWIPTAPLHVQLYHALGWEMPIFAHMPLILAPTGG
                     KLSKRHGGSSVREFREAGYLPEALLNYLALLGWSLDDHTEI"
BASE COUNT         2365 a         4624 c         4989 g         2337 t
ORIGIN      
        1 ggcgggcggt tgcccatctc ctcgcccttg atgaggatct ggccgctgtc cggatgctcg
       61 aacccgccga tcatgcgaag ggtggtggtc ttgccgcagc ccgaggggcc caggaaggtc
      121 acgaacgcac cgctcggcac ctcgaggtca atgtagtcga cggcgaccag gttgccaaat
      181 cgcttgtaga gctgacggat gctgacatcg aatgcggaca tgtgccgtga ccccttgtcg
      241 cgccgaaatc gatcactgcg tgagaagagt gtagctcccc tcgcccgtgt tccgcagcat
      301 tcgaagcatc ggcataagcg atccgtgcag acgaatcacc ggtcgggaca ctgtcccgac
      361 cggtgtcgac cagcagcgag tgggccgctc ctaggagagc ttgacttcgg tccagagctc
      421 gtcgatccgc tgccggactt ggggatcgag gtcggtggtc agcaccagga tcgactcttc
      481 cggcaccgcg aactgctcgg ccagctcggg ctccagctcc tcccgggccg ccctgttgag
      541 cgtgtcgtag tacatcatcc gcatctccat cgccatgata tcggcgcgat gcaggaagtt
      601 gatgaacttg tgaccgttct ccgggttctc cgatgtgctc gggatggcga aggtgtcgat
      661 gaaggtgagg acaccctcct ctggaacggt ccagtcgatc ggcgcgccct cggccgcgcc
      721 catgcgggtg cggccggcgg tgagctgtcc gacgagcgct tcctcgttga tgaacatctc
      781 catgccgttc tggtagtcgg tccagtactt gaacacgagc tccttctgct cgaggagcag
      841 ctcacggatc cggtcccact cggtcggctg aatcggatcg tcaccgatat acagcgccgt
      901 cgtgccgacg cgggcgtagg cctggtcctg catggtgatg cgaccgtcgt actgcgcgtc
      961 ccacgtgacc ccccaagagg tcggcgcctc gggaaattcc tcggtgttgt aggtcaggct
     1021 gtagtacacg cgggagaacg gcacggcgta ggtgtttccg tcctgctggt agatctcgtt
     1081 ctcctggaac tcctcgtaca ggttgtcgat gttgggaatc tggccctcgt cgagcggatg
     1141 aagcagattc gccgccatcg cgcgctgcac ccagtctgtc gaggggatga agaagtcgaa
     1201 gttctcaccc gcgttgaggc gtgcccacca ctcatcgttg ctggagtaag taccgagctg
     1261 ggtgacattg atgccggcct cctcctcgaa gatcggcacg acctcggcga tccacggctg
     1321 gtaccagttg tagatgacga gctcgccatt gtcctgacgc agattgatgt tgaagccggg
     1381 gttcccggtg gaggcctggg cgatcgctgt cgagagtgcg gaaccaccgg cggcggctcc
     1441 ggcggcgccc ttgatgagcg agcgacggtt caggcccttc ggttgcatat ccttcttgcc
     1501 catggtgcaa tcctttccat atgtgacacg tgccggtgcg ggtcctgcgc ggattggctt
     1561 gcgggagctc ggcgggtggc agacccggca ttgtcggtgc cgtcgtatca gcaatggtgc
     1621 tgaccatgga acgtcctgca agattcaggc cgcccgaacg ggcccggtga gccttggcgg
     1681 cgcacctaca gctccctgat ggagtgacgt cgtgcagatg cacccccaga tcacgttccg
     1741 gccgctcgtc gatgccgatc ttccggccat gaagcgctgg ctggaggatc cggacgtcgc
     1801 gtggtggtac caagcgggct ccacagcagt cgaggcgctg cgcgagcact accgggacga
     1861 gatcgccgga acgagccgga cgcgggcgtt catcatcggg ctcgacggcc gcgacgccgg
     1921 gtatattcag tgctacgcga tcgacgacga gccgaagtat gcccagcaga tcaaggtcga
     1981 tcccggcgca gtcggaatcg atctcttcat cggcgagtcg tgggcgcgca accggggcta
     2041 cggccgtcag gtgttgcggg cgttcctggc gcagatcgtc ttcgtgaaga tgggggcgga
     2101 ggtggcgatc atcgcgccgg aaccgggcaa cacccgtgcc gtgcggtcgt acgagcgggc
     2161 cgggttcgtg gggtggaaga tcgtctacgt cgtcgacaaa gacgcgccac aaaacaccgg
     2221 acaagagtac atcatgcgcc tcacacggga agcgttcgag tcgggggagt gaggagatgc
     2281 cttccctcgt ctcacttcgt agggcccgac ctcgtgtcat cccgaagtcg caggcccagc
     2341 ctgttctgga aagcaggaac gacggaagcg cctcgatttt gaagctggtg tgacgaaggg
     2401 ccccgatgcc ccgatccaat agccttaccg gggcgtgccg ggctcgccga gcaggcggac
     2461 gacgaccagc agcatcgcgg agccggccag cagcaccacc gagagcagca gtgccgtggg
     2521 caggtcgcct gcctggaaac gctcgtagat cgccaggggc atcgtctggg tcacaccctg
     2581 gcggttgccg gcgaacagga tcgttgcccc gaactcaccc agtgcccgag cccacgccag
     2641 cgccgctccc gccgcgaggc tggagcgggt catcggcagc accactcgcc agaagacccg
     2701 cgacggcgct gcccccagat cggccgctgc ctcctccatc cccacatcga cccggccgaa
     2761 gccggtccgc gccgtacgga tgtagaacgg cgcgccgacg aagacctgcg cgatcaccac
     2821 ggccgccgtg ctgaacccga ccgagatgcc gagtgcgtcg agctcgtcac cgagcaggcc
     2881 ccggcggccg aacgcggaca ggagcgcgat gccggcgacg gccggcggca ggatgatggg
     2941 gaggtcgatg agggagtcca gcagccggtg ggccggcagg cggtagcgcg ccagcatcca
     3001 tgccacgggc gttccgatcg cgacaatcac cacgatcgcg atggtcgagg tcatcaggct
     3061 aagtcgcaga gcggcgatca gcgtctcccg ggtacgctcg tcgtcgatgg aggggttcgc
     3121 atcgagcgtg cgcgccagca gcgagatggt cggcagcgtg agcgcggcaa gagcaacgag
     3181 cccgagcgcg atgattcccc atgccgaccg ggagatggta cgggatgggg gataggtgct
     3241 gggtggcccg gtaccgtctg tcatgcgttc ccttcggttt cgcgcgaggc tcacgggacc
     3301 ggcgcgaatc cgtaatccgc gagaatccgc tgccccgcgt cactgagcac gaacttgatg
     3361 aacgctcggg cagcttcggt gttcccgccg acggtcggcg cgatcggata ggtggcctcc
     3421 gtcggaacat cgctcgggaa ctcgaccact ttcacctctc ccccggcgag ctcggcagcc
     3481 gcggcatcgc tggcgtagac gatgccggcg tccgcctcgt cgagctggac cctggtgagg
     3541 gctgagcgga catcggtctc ctccgacaca acgttggcgc cgatggcggc aaccgcctcg
     3601 tcatcggcgc cagcccagtc acagatcgcc tgccgggcgt agcgaccagc ggggacgtcc
     3661 tcggccgcaa tcaccagccg gatgtcgtcc cctgcgaggt cgtcgatgcc ctcgatgccg
     3721 gcagggttgt ccggcggggt gacgatcacc agccggttgg cggcgaagag cacgggctca
     3781 ccgttgacca gccctgattc ctgtgcctgc cgcatggtgg cgaggctggc ggtcgcaagc
     3841 acatcggcct cggcaccctc gccgagctgg gtcacgagcg tctgggatcc cgccgtttcg
     3901 acggagatcg acacattcgg atggcggggc tcgatctcgt cagcgatatc gacgaacgcg
     3961 tcggtcaggg aagcggccgc gaagacggtg agttcgattt cgtcgtcggg aaacgcagcg
     4021 ttctgtgcct cccgcttgga tggcgtgccg gccgcaggag gcgttgccgg gggcgggcag
     4081 gtgagttcga tggcgcccac tgacggagca ctggccaagg aggtcatgag catgacgagt
     4141 accagagcta tcgtgagcgc gcctggacga tggtgcatcg aagcttgcct ttgcgaagga
     4201 atggccctag tcagcctctg aataggtatc gtagtgcccg gcagggctgg tcgcaacccg
     4261 gcccatcctc acccggcgga acggctgcac gagaggcgac ctgcaacgtg cactcggtgg
     4321 cattgcgctg gagctagcgc ttcgcaagtg tcttctgcag cgagcgtcgc gtgatcttct
     4381 cccacgcctc gggatccgtc gcgccctcag tcgcgaggat cagcacgctg gcgcgcttgt
     4441 caaggtgcag gcggctgcgg agattggtgg catccgaggc gctggcgatg gcgagcagcc
     4501 ctgcgaggct ggcggcgccc gtttcgccag ccaccacccc tttccgcgcg aggatacgca
     4561 tcgcctcctt ggcggcgtcg tccgggatcg agatcgcggc ggtcagcccg gcagccagca
     4621 ccggccacgc gatcggcgag ggctggtcgc agttcaatcc cgacatgatc gactcgtgcg
     4681 ggccggggat ggcgaccggg gcaccgcgct ggagtgactt cagcacgcag ggcgcgccat
     4741 ccggctcgac cccgatcacg gtcgcgggag gcatgcggtg gcgcgcggcc acgatcgcgg
     4801 cggcggcgag cgcgccgaca cccatcggta cgagggtgtg cgatggcgtc agccggcact
     4861 gctcctgcag ttcgtggaac atggtgctgt agccctcgat cacccagcgc ggtatcaggt
     4921 cgtacccgag ccacgccgtg tcggagatca cgaggcagct gtccgaggcg agctcggcgg
     4981 accgagctac ggcatcgccg taggtgccct cgacgatctc gactgtcgcg ccctcaccct
     5041 cgattgcctc gatgcgggcc ggcaccatgc tggacggtac gacgatcgtg gcctcgcagc
     5101 cgagcatggc ggccatacgg gccacggcgc gcccgtgatt gccgtcggtg gcggcggcga
     5161 agcttatcgg ctcgcgggcc tgcacctgcg cgcgcaggtc gtcgatggtg gcccagttgt
     5221 ggtcgatatc cggccagcga gccagcaacg cccggtagat cgcccagctc gcgcccagca
     5281 tcttgaaggc cgggagcccg aggcgggacg tctcatcctt gacaagcacc tgctcgacct
     5341 tgatctcgcc ggcgaggtcg tgaaggtcat ggattggcgt cggctggtag tccggcaggc
     5401 ggcggtggaa ctcgatcggg ctgatgggga ggggtgacgc ggggtcgaga tccgggctca
     5461 gcaccacggg tcgctgccgg aaccagattt cggtgagcgc agtggtcatg cattgttctc
     5521 cgtggtgtcg agtgaatcca gccattcgag tgccgcctcg gtctgtgcga gcctgagccg
     5581 cagcaccatc cggcgctcgg cgtccggagc ggcggtcccg acctccatct gggccgtcag
     5641 cgatcgcagc aggtcggtca ggaccgcccg ctgctggccg atcaggtggg cgagccgggc
     5701 cggtgccagc cgccgggtga agaagagctt gaggagaaag tccagccgaa tctcgcgggt
     5761 ggcgtgcacc ggctcctgca gccactggtc gagtgcctcg cgaccgtcca gagtcagggt
     5821 gtggatgcgc cggtccgggc ggtcgacctg ccgctcgatc tcagtggtga tcagcctgta
     5881 gcggtcgagt ttcttgagat ggtggtagag catccccggc tccagccgga taatctctcc
     5941 caatgccccg tgggagaact gccgggcgag atcgtagcca tggatgccct cttcccccgc
     6001 gccggcgagc agcccgagca ggctgtgctc ggcggggccg agtcgcagcg cgcgacgcgg
     6061 tctgctggcg gatcgtgggc gatcggtcat gagccatgtc cttcgtcgtg tgggtgtcag
     6121 gccgggatcc gtagcgtgtc cctagcgccc tggtcctcct caagcagtgc cgcacggccg
     6181 agcaggcgca cgcgcgccac gcgcaagcca tccagcgcct cgacgacgat tcgctcgccg
     6241 ctggaggtct ggacctcgtc atcgatctgc gcagcgcgcc cgagtcggaa aaagacgtag
     6301 ccaccgatcg tatcgacatc gaggccgtcg tcagccaggt cgatgccgta ctcgtccttc
     6361 aactcggaga gcgatgtcag gccgtcgagg tggatcgtac cgtcggcggc gagcccttca
     6421 gatccgctgg cctcgtcctc ctcctcggtc aggcggccga gtagccggtt gacgatatcc
     6481 tgcagggtga cgatgccggc ggtgccgcca tactcgtcga tcaccacggc catctggacg
     6541 tgggtctcct gcatgcgagt gagcacctcg gagacggcga gcgtttccgg caccagcgtt
     6601 ggctccacca tgtagtcact caggcggaag gtatcggcga cggtggtgcc gttaggatcg
     6661 cgcgtgaccg catgcggcgt caggccagtg atgtcagcgg tgccgttcgc ggcgcggtcg
     6721 ctggactcga cggtggcgat gtgcggcgcc gagccgttcg gcagcgccgt ctggggcgca
     6781 cgctgcgccg cccactgcag gaagagcggc agcatgcgtt tggccttgat gatgccgatg
     6841 atgttgtcgc tgtcaccctc gtagaccggc aagcgggtgt acgacgtatc gaccgtcatg
     6901 cgaaccacgt cgtcgagcga ggcgtcagcc ggcagggcag tcacctcggt gcgcggcacc
     6961 atcacctcac gcgcctggag gtccgtgaag ctgaaagcgc ggtcgagaat gtcgtgggcg
     7021 gtttgcgtca cgaggccagc ctcgcggctg gcatcgagcg cgatcataag ctcctcggcc
     7081 gactgcacca gctcgtgccc ctgcgccggc tcgaagccga gcatgcgcac cacctggttg
     7141 ccgacccagt tcagcgcgtt gatgaccggc cggaagacga tcaggaacca gtggatcggt
     7201 tcgccgacga gcagcgaggt ctcctccggg cgctgaaggg ccaggctctt gggggccaac
     7261 tcaccgagca cgatatggag tgccgtgacg atcgagaagg cgactacgaa ggagatggtg
     7321 tgcatcaccg tcgagctgac ctcgaacggc agccgctcga tcagcgggtg caggacgtgc
     7381 gcgagggcgg gctcgccgat ccagcccaag gcgaggctgg ccatggtgat gccgagctgg
     7441 gtggcggcga tatacgtgtc gaggtggtcg atacggtcga gcaccctggt ggcgcgggag
     7501 tcgccttccg aggagagttg ctggatgcgg gtgcgtcgga cggagacgag ggcgaattcg
     7561 gtcgcgacga agaaaccgtt cagcagaacg agcacaataa tggcgatgag cccgaacgca
     7621 gtactaatgg ggtcctccag accaaagggt gggcggtgtg aacagatgct cgcgaccgtg
     7681 ccgatcggca cggcacaacc acggttcatc caagggcaag cgcaccgctg acgttatggt
     7741 cgattctagt cgattctagc cgttatctca acggaatccg ggaaccgccg gggctgcccg
     7801 agatgtgcat gctacgatca tccgggtggc agctcaccgg gcgcgcagga gggtgtgggt
     7861 cagcgcatga gcgggacagg tcgaccggca tccgagcgtc tcgatcacct cgccgcgctg
     7921 ctgtcgtcgc aggttcgcgc cgaggtgctg gtgcatgccg tcacgcgagc cgataccggt
     7981 cactcgctga ccgagttggc ccacgggctg gggctggcga tcagcagcgt ccagcacgag
     8041 gcgtacaagc tgcaggattt aggcctcttc tcgctgaagc gggagggggc gtcgcggcgg
     8101 tactcgctcc ggctggagga gccggtggtg caggcgctgc gggagttggt ggtcgcgact
     8161 gtcggcgcgc cgcgggtggt cgaaatggcc ctgaccgatc tccccgggct cgcggcggga
     8221 tcgctgctcg gggccatccc gccgcggcag ggagagaccg cgctggtgct gatcggcgat
     8281 ctggagctgg agacggtcga gcaggcccac tggcgggcgt cgtggctgct ggatgcgcca
     8341 gcggaggcgg tcgagctggc cttctttcgc ccggcggact ggctcgcgcg gcgagacgcc
     8401 ggtcacccac tggtgcgacg gctgctggac ctgcccgtcc gcgccgcctt cggcgacgtg
     8461 cgggttgacg agcaaccata gcaggctcaa cgtcatgctg agcgagggcc gcaggtccga
     8521 agagaaagca tccccgcgcg gagcgcagcg tggcgggcgt cgggtatcga acctgcgtaa
     8581 cgacgtgcgg aggcggcagc aaagcttcgg gcgcgtggcg tggggacctc gcttcgctgc
     8641 ctcggcacgc ggactccgta cgcggctcgg agtgatggtg aattgggcta cggcgagtga
     8701 cacatagccg agaggggagc acacgtaatg actgagcagc aggcaccgcc gacaatggtg
     8761 atcgggcagt ccggcggggc gacagcggtg atcaacgcca gcctcgccgg cgcgctggag
     8821 gtcgggctgg ccagcgacga ggtcggccgc atcttcggca tgcgccacgg catcgagggc
     8881 ctgctcaaca gtgacctcgt cgacctgggc aacatcgacg ccggcaggcg cgacgtcctg
     8941 aagcgcacgc cctccgccgc gctcggcacc gggcgctaca agctcaagga cgacgacctc
     9001 gatcccatcc tcgacatctg cgcgcgcgag aacatccgct ggatcctcta catcggcggc
     9061 aacgactcgg ccgatacctc ctcccggctc gcgcgccatg ccgaggcgcg tgggcaggag
     9121 ttgagcgtga tcgcgattcc gaagacggtc gacaacgacc tgccggaaac acaccactgc
     9181 cctggctatg gctcggtcgc ccgctacctc gccaacaccg cccgcgacgc gatctacgac
     9241 acgttcgcct cgccgcagct ctacgcggtc aagttcattg aggtgatggg gcgcgacgcc
     9301 gggtgggtca ccgccgcctg cgcgctcgcc acccatggtg acgaggctga cctctcgccg
     9361 ctgttgctgc tgccggagcg cccggtagag gacgtcgacg ccatcatcgc cctgatcagg
     9421 gccgatatcg caaggcgggg ctggtcggtt gtggtggtgc cggagacgtt gcaggaccgc
     9481 gaaggacggc acctcggcgg ggacgagccg gagttcgtcg atgcgttcgg ccatccgtac
     9541 taccccagtc ccggcgcggc cctgagccgt gtgctgacca gtgccgggct tcgggcgcga
     9601 tacgacaagc caggaacggc ggcccggatg tcgaacgcga tggcatcgcc ggttgatctc
     9661 gccgaagccg aggcgctggg agcagaggcg gcgcggcgcg ccattgtcgg tgagacggcg
     9721 cggatgacag cgttgcgtga gaccgatgaa ggccggatgg agatcgtggc ggtgccggtc
     9781 gagcagattg ccaaccgggt gcgcctgctc gacgacgcct tcattggcaa ggatggccac
     9841 agcaccaccc aggcgttcct cgactatgcg ctgccgctcc tcggcccgga ccccttcccg
     9901 ccctacgcgc gcatctgacc gttactgcct gcccgcaggg gcggatcccc gacggcattg
     9961 tccacctgtc catggtcgtg ggcgccagca tttgcggggg gtgctgtcat cctgagccgc
    10021 gactgccttg ggcgggcgtg tgtcgaagga tctcttgcct gctgtgcagc agtgctcgaa
    10081 cggaggcacc accttcgcaa gagatccttc tccgaacctt cgatcaggct gaaaccgcca
    10141 gcttgctgca aggagcgggc gttgatctcc caaacgaaga gccggcaggc atccgctacg
    10201 gtctgcctct atggatggtt cgctcgcatc agggatcacc ggtcgcctcg ccgtatactc
    10261 aaggcagtgc gaatccgcat gcacccgtcc gggcgtgccc cggatggtgc cagggagccg
    10321 aacgccatgt ccaaacgcga cgaccagaag cggctgccac gctaccgcaa tcccgacgcc
    10381 ggcctgaccg atctgatcca gcagagccac gccctctcga ggccgctcgc cggcatcatc
    10441 cgttatggtg aggccgatga ggagaccggc gaggtcgacg agcgggagat cgatgtcggc
    10501 gccgacgtct tgagccacga tggcgacaaa ctgggcgagg ttgtcaacgt gatggacgac
    10561 tacctcgtcg tcgagcaagg gttcttcaac ccccacgacg tctacattcc gatcagtgcg
    10621 attgcctggc acggcgatga ctgcctgtac ctgcgcatga cccgcgacga gttcgaggcc
    10681 tccgactgga ccatcgagcc ggctccgaaa gcggaccacc tgaggaaccc gcatgacacc
    10741 ggcgaggaac ggtagaccgc catccgggga ggggagcttg tggagcgtgg cgttgtccgg
    10801 aacctgctcg gtcgcctcaa cgcccggccg ccaggcgata tcctgcgcat cgccgagcgc
    10861 tggcaggtac ccctgagcgg cactgatact cgccggcacg tcggcgcgct ctaccgcacg
    10921 atgaccgatg tccgcgccgc ccggaccttc ttcgacaacc tctccgacga catccgcgcc
    10981 gtcgtgcaga ccatggcgct cgccgatggg cggctgtaca cgattgccga gctggccagg
    11041 agcatcgacc gccccgaaaa ggcggcacgt gacgctgccg tctggctctt ctacgccggg
    11101 gtgctcgctc gcgagggcga ccggcaggaa ctccccgtcg gtgcgactcc acgcctcttc
    11161 ctgccgcgtg agcttgagca ggtgttcact cgcgcgatcg acgagatcga ggttggcgac
    11221 atgcggcagg agtcgctgcg ctcactgctg gcgctcctcg acgacagtga tgtcgaggaa
    11281 gccgcgagcg actggggcat ccaggtgatc cccgggctgc gcagccgcga cgaccttgcc
    11341 gaggacctgc tgcgtctgat ggacgagccc gaccgcatcc gtcgcgtcgc cggagcgctc
    11401 ggtcgcgatg cgaagtcgct ctgggaggtc gtgcgcgagg catccgaggc ggacgggggc
    11461 atgccctacc acgacgcgct ggtcgccgct gggctgttgg gacaggatgc acagcggccg
    11521 cgcaacgcgc gtgaggcggg gcacattctt gagacgctgc aggaggccga aggcaagctg
    11581 ctcgtctggc actcctacga caatgagggc aaccgctggc tcttcgtgcc ccaggagatc
    11641 cgtcaccccg gccagcgcgt ccgcacgctg gagctcaagc cgctgacccc cgtgccggag
    11701 aaggaggtga caccttccga accgcatcac ccgcacgccg tcgcgtggga tctgctcacc
    11761 atgctacggg agctaatcag ccatcggtct ccgggctgga agccggggga gccgctggcg
    11821 cggggctggc agcgacagat cggcggccac ctctggctga gcgacgacgg tgtccccgag
    11881 gaggggtatc ctggctttct gctctcgctg gcacaccgag tcgggctggt cgagccgggc
    11941 gagaagccgg cccgcaccgg cgccgagaag ggcgcgtttc gacccaaggt gaccgacgcc
    12001 atccgtggtt ggcgcgggca gtcattcagc tcccagaccg ggcagctccg cgcggcgtgg
    12061 ctcgaccatg aggactggat cgaggggcgc gagcgcgacg aggtcgagat ctggggcgca
    12121 tcgtggccgg acgtccgccg ccggttgttg gaggtgctcg gctcgctgga gcccggcacg
    12181 tggtaccggc agcgtgagct cgccctccat ctggcacagc agcacccggg gttgctcggc
    12241 tcgaccttca ccgtggcctc cgcgcgtggt ggcgacgccg atgagggccg cgagggccgc
    12301 attgccaagg tcgcgcaggt gatcggcatc gagctggagt cggcgttcca ctggttcggg
    12361 ctggtggaga tcggtgttac gggtaccggc acgaaacgca tgcgcacggc ccgcctcacc
    12421 gaggcgggag agcttgccag ggagcgcgcc gaagtggtgc cgggcgacga cgcggccctg
    12481 cctggtgccc cggagctcga cgccgatgcc ccggtgatca ccctcgacga ggcaggcgaa
    12541 gtcaccctca accggccctc accgctgcat atctggtcac tctcggcctt cagcgacccg
    12601 gtcgatctca ccccgatggc gcggtatgta ctcaccaagg cgtcgctcgg gcgggcgctc
    12661 ggggcagggt tcgacctcga tcaggtcgtg cagtatctgg agcggcagtc cggcgagccg
    12721 gtgccggagc cgctgatgca gcacctgcgc gagtggacgg ttggctaccg ccgcgtgcgg
    12781 ctgcggcgat tggccgcggt gcagccggac gacaccgagc tgctcgacga tctggcgagg
    12841 gtgctggagg aggcgggctt cgagatcgcc gaccggctcc cggaagggga tggtctgcgg
    12901 gtctggctgc cggagacggg cgatgatgcc gccacggccg aggacgccct ccagcgcgcc
    12961 ctccgcgcca atggcttcgt cggccagtgg ccccgccgcg ccgaacccct tccccgtcgc
    13021 ccgcgagact gaccgaaaac cggcgcgggg tatactttcc gctgatttgc atggccatgc
    13081 gggttgatcg gcctgatcaa ttcgtagggg ccgacatcca caggagtcgt cggtccagct
    13141 gtccatagcg cggcaaggca acgatgtcgc cataatgcgt gcttcgcgca cgccgggccg
    13201 ggtggacaac tgcaggatgg ctgccaggtg tgcgccgtag ggacaggcct tgtgtctgta
    13261 cgggcatgtg tcgccctgcg gaggtcgggc cgactccatg tccgctctgg tcgttacggc
    13321 caatatccca ttgcatcgaa cgaaaacgag aggaaaccgc ctccatgact gacgagcaga
    13381 agatcattgc gaccgaggac cggccgaggg cgatcccggt gggcctcgac gaggtgcgtg
    13441 tacggttcgc gccgagcccg accggctcgc tgcacatcgg cggcgccaag ctggtgatgt
    13501 acaacgtgct cttcgcccac gggcaggcga agcgcgaggg caagaagggc accttcgtcc
    13561 tccgcatcga ggacaccgac cagaagcgct tcgtcgaggg tgccgccgag aacctgatgg
    13621 acgagctgcg gtggctcggg ctcgagtggg acgaggggcc ggacgtaggc ggaccgtatg
    13681 cgccctacgt ccagagcgag cggcagcacc attaccgcga ggcggccgag cgcctggtcg
    13741 aggaggggca cgcgtaccgc tgcttctgct ctcccgagcg cctggcgcag gtccgagagg
    13801 atctgcagaa gcagggcctc ccaccgggct acgaccgcca ctgtcgcaac ctcccgcagg
    13861 aggagatcca ggcgaaactc gacgctggcg agggctacgt tgtgcgattc aagatgccgc
    13921 tcgaaggcga gacgcgcttc accgacctgc tgcgcggcga gatcgtctac gagaacgaga
    13981 agctcgagga cctggtgatc ctcaagtcgg acggcttccc gacctaccac ctcgccgtcg
    14041 tcgtcgacga ccacgagatg gccatcactc acgtcactcg cggccccgaa tggattccga
    14101 cggcacccct gcacgtgcag ctctaccatg cgctcggctg ggagatgccg atcttcgcgc
    14161 acatgccgct catcctagcg ccgaccggcg gcaagctctc caagcggcac ggcggctcat
    14221 ccgtacggga gttccgcgag gccggctacc tgccggaggc gctgctcaac tatctcgcgc
    14281 tgctcggctg gagtctcgac gaccacaccg agatc
//
LOCUS       DASYWL010000264         2081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_237725, whole genome shotgun sequence.
ACCESSION   DASYWL010000264 DASYWL010000000
VERSION     DASYWL010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237725"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VGR22_09535"
     CDS             complement(<1..531)
                     /locus_tag="VGR22_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2128846.1"
                     /translation="MNAMLELRDVHTYYGQIHALKGISLTVNQGEIVTLIGSNGAGKS
                     TTLKTISGLLRPRSGQVLLEDNVISGRPAHTVAELGVAQSPEGRRVFPRMSVLENLQM
                     GAFALGGPRREDLERVFDLFPRLKERVSQKAGTLSGGEQQMLAIGRALMARPRVLLLD
                     EPSMGLAPILVEQIFRI"
     gene            complement(528..1442)
                     /locus_tag="VGR22_09540"
     CDS             complement(528..1442)
                     /locus_tag="VGR22_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2128847.1"
                     /translation="MVAEATSRTVTAVEATGTPLLETRDLTQRFGGLVAVDSVSLAIP
                     HGRIASVIGPNGAGKTTLFNVIAGIYPPTEGEVLVDGDVIMRSGGAGRRRSRRPDQVM
                     ELGIARTFQNIRLFANMTAIDNVAIGMHSRMHATPLGAILRTRSVREEERRVRERAIE
                     LLDYVGLGRHADAVAKNLSYGDQRRLEIARALASDPKLLLLDEPTAGMNPQETAGMTT
                     FIRRLRDDLNLTVLLIEHDMKVVMGISDQIAVLDHGIKIADGTPEEIRSDPQVIEAYL
                     GAGAAETADRLAQRQTDIEPITSETDNR"
     gene            complement(1457..>2081)
                     /locus_tag="VGR22_09545"
     CDS             complement(1457..>2081)
                     /locus_tag="VGR22_09545"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV2128848.1"
                     /translation="EITFTTGDQRNWFWLILVVGLVSLFMITRLYDSRLGRAWQAVRE
                     DEIAAASMGVNLVRTKLWAFALGASFSGFAGAVYSGYVRVVYPDQFQFSVSVIILCMV
                     ILGGLGNIYGVIAGAMIIGAFDRILAEELSRPVNWLGEQLEWQPLIEHNVTQDRFLIF
                     GLALVILMLTRPGGLFPQRARAEELEPDSTHISEQERDSLADASGRI"
BASE COUNT          367 a          678 c          668 g          368 t
ORIGIN      
        1 aattcggaag atctgctcca cgaggatcgg ggcgagcccc atcgacggct cgtcgaggag
       61 cagcacgcgc ggccgcgcca tcagcgcccg accgatggcc agcatctgct gctcgccacc
      121 ggagagcgta cccgccttct ggctcacacg ctccttgagc cgtgggaaca gatcgaagac
      181 gcgctccagg tcctctctgc gagggccgcc gagggcgaag gcgcccattt ggaggttctc
      241 cagaaccgac atgcgcggaa agacgcgccg gccctcgggc gactgcgcca ctcccagctc
      301 ggcgacggtg tgcgcggggc gaccgctgat gacgttgtct tccagcaaca cctgaccgct
      361 tcgaggacgc agaaggccgg agatcgtctt gagggtcgtg ctcttgcccg ccccgttgga
      421 accgatcagc gtgacgatct cgccctggtt cacagtgagc gagatgccct tgagggcgtg
      481 gatttgcccg tagtaggtgt gcacatcgcg aagttcgagc attgcgttca tctgttgtcc
      541 gtctcgctgg taattggctc gatgtccgtc tgccgctgag cgagcctgtc ggccgtctcc
      601 gcagctccgg cgccgaggta ggcctcgatc acctgtgggt cgctgcggat ttcctcgggc
      661 gtgccgtcgg caatcttgat gccgtggtcg agcacggcga tctggtccga aatgcccatg
      721 accaccttca tgtcgtgctc aatcagcagt acggtcaggt tgagatcatc gcgaagccga
      781 cggatgaacg ttgtcatccc tgccgtctcc tgcgggttca tgccggcagt cggctcgtcg
      841 agcaagagca gcttcggatc gctggccaga gcgcgcgcga tctcgagccg ccgctgatcg
      901 ccataggaga ggttcttggc gactgcgtcg gcgtgccggc cgaggccgac gtagtcgagc
      961 aactcgattg cgcgttcgcg cacgcggcgc tcctcctccc gaacggagcg ggtgcggagg
     1021 atggcgccga gaggcgtggc gtgcatacgg ctatgcatgc cgatcgcgac gttgtcgatc
     1081 gccgtcatgt tggcgaagag ccggatgttc tggaacgtcc gcgcgatgcc cagctccatc
     1141 acctgatccg gccgtcgaga cctgcgtctg cccgcccctc cggatcgcat gattacgtcg
     1201 ccgtcgacga gcacctcgcc ctcggtcggt gggtagatgc cggcaatgac attgaacagc
     1261 gtcgtcttgc cggctccgtt cggtccgatc acgctcgcga tgcggccatg cgggatcgcc
     1321 agcgagaccg agtcaaccgc gacgagcccg ccgaagcgct gggtcagatc tcgcgtttcc
     1381 agcagcggtg taccagttgc ctcaacggcc gtcacggtac gcgaggtagc ctctgcaacc
     1441 atcgtcacca atccttctaa atccggccac tagcgtcggc caggctgtcg cgttcctgct
     1501 cggagatgtg cgtgctgtcg ggctccaact cctcggcacg cgcccgctgc gggaagagac
     1561 cacctggtcg ggtgagcatg agtatgacca gggccagacc gaagatcagg aagcgatcct
     1621 gcgtcacgtt gtgttcgatc aagggctgcc actcgagctg ctccccgagc caattgaccg
     1681 gcctgctcag ctcttccgcg aggatgcggt cgaacgcgcc gatgatcatc gcaccggcaa
     1741 tcacaccgta gatgttgccc aagccgccga ggatcaccat gcagagaatg atgaccgaca
     1801 cgctgaactg gaactggtcg gggtagacga cgcggacgta cccggagtag accgcgccgg
     1861 cgaaaccgga gaacgacgcg ccgagcgcga aggcccacag cttcgtccgc acgaggttga
     1921 cgcccatgct cgcggctgcg atctcgtcct cccggacagc ctgccaggca cggcccaggc
     1981 gcgagtcgta gagacgggta atcatgaaga gcgacaccag cccgaccacg aggatcagcc
     2041 agaaccagtt gcgctgatca cccgtcgtga acgtgatctc g
//
LOCUS       DASYWL010000265         4353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_464062, whole genome shotgun sequence.
ACCESSION   DASYWL010000265 DASYWL010000000
VERSION     DASYWL010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464062"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            381..1415
                     /locus_tag="VGR22_09550"
     CDS             381..1415
                     /locus_tag="VGR22_09550"
                     /inference="COORDINATES: protein motif:HMM:NF033206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HEV2128849.1"
                     /translation="MLHVALAGSGGETPGSPAFEPPFGPVLGGPTAAVAQIDDQGCPD
                     AVVTGLPSYLDGTGGVIGVADVIFIDGQLYFLEAAGTEVYANPDTTTGLFRATDDGTG
                     ELVADYAAWLEENPPEEAGWVQPPDIPEEFPNPGNPYAMAFVGDELWMVDAVNGLVVS
                     MGLDGTIELVADLSRRHPVPTGIAPAPEGGAYVGTLTAVPYPDEVAKVVHIAPDGMVT
                     DAWTGLTMVTAVAVDASGTLYAVEMSTDNMEEPPFVVPDSGRVVRQTGPASLEPVAED
                     LLFPIALSVGPDDALYVALPAVGADTGSGMIVRLSDSDGHPTMDETSCAPLAETVNAP
                     QGPVEATPAT"
     gene            1645..2616
                     /locus_tag="VGR22_09555"
     CDS             1645..2616
                     /locus_tag="VGR22_09555"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEV2128850.1"
                     /translation="MDRRMKWARRLAVITAVGMFLVLLMGANVTATGSGEGCGRSWPL
                     CNGSVLPAQTLESFIEYSHRIVTSIEGIFVAATSIVAWPLRKRFPQFKALVPAVAGTL
                     ILQSLMGAAAVMWPQTPEVMASHFGISLVCLAAAALIARIVTEARDNDEHLEVRRITA
                     PTGYRLLMGVTFIFSIVVAYSGAYVRHTGAELACTSWPTCNGELIPEFGGLAGNQTLH
                     RLAALGISLMIVTLLVWSYRFRAEREDLHRNALLAFILVIAQSIAGAIVVFSGLQLLA
                     ALTHAALMALLFVVLSDGLRLVLPPPERQIDVETVQCGSVPRMATGD"
     gene            complement(2833..4047)
                     /locus_tag="VGR22_09560"
     CDS             complement(2833..4047)
                     /locus_tag="VGR22_09560"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HEV2128851.1"
                     /translation="MTLLAVIADDLTGAGDAGGAFATAGLRTVILFGNERSEDADVIV
                     RSTDSRGLDTTDAAEVNRRAVRGLRCPPPRQPPRWVYKKIDSALRGHPCEELLAVMRE
                     LGERRALVAPALPAQGRTTAGSRQLVDGIPLDQTPFGVPASGADLISVFGCERQVAIQ
                     PITLATVRHGTGHVAHAIEGLEEGILVADAETDRDLMTIAGAALDNGIRVMAGSAGLA
                     RQLATTLPAPVSARRAATPDCRTGPILVVAGSRHPSAAAQVAALVEAGVPLVHPSQEM
                     LDGTAPVDTTVAALAPHLFESRFVALTTTGLAASPKGPEFVVRQLAAIVAALAERHLV
                     GGLVLTGGDVAAGVLARLKATEIMLGGEVQAAIPWGILRSRLLPEVPIVTKAGSFGDR
                     GTILRCLKHLER"
     gene            complement(4044..>4353)
                     /locus_tag="VGR22_09565"
     CDS             complement(4044..>4353)
                     /locus_tag="VGR22_09565"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEV2128852.1"
                     /translation="VSDPQPPDTVFLRATKGEWDIVVAMYHDQGHIPMKLLAFDSGVN
                     VSIGLPILRTSVDHGTAFDIAGTGKASESSMLAAIEVAVQLAASRSASQEAPSRGSRR
                     "
BASE COUNT          685 a         1437 c         1472 g          759 t
ORIGIN      
        1 cgacatcttt ctcgggatca acccggctgc cgtgtcgtcg gaaacgctgg accgctaccg
       61 ggagctcggc gtgacgtggc ttgggtctta cctgctgaaa gcggagtacg ggcacgaggg
      121 caacctcgaa cggattcgtc aagggccgcc ggccgcctga tcggtgtcgt gcctagagtg
      181 gccgagccga caattctcga catccgaagg agattcgaca tgttcctatc gagacgattg
      241 ctgacactca tcaccgttct cttccttgcc acgccgctga tcagccaggc tcatgaggcg
      301 accccggcgc ccggcagcga cgtggaagtg gtcgcgagcg gcctcaccaa tccccgtggc
      361 atggcctgcg gccggacggc atgctccacg tcgccctcgc tggcagcggc ggtgagacgc
      421 caggcagccc agcgttcgag ccaccgtttg ggccggtcct cggcgggccg accgcagcgg
      481 tggcccagat cgatgaccag ggctgtcctg atgccgtcgt caccggcctg ccgtcctacc
      541 tcgacggcac gggcggggtg atcggcgtgg ctgacgtgat cttcatcgac gggcagctct
      601 acttcctgga agccgccggt acggaggtct atgccaatcc cgatacgacg accggccttt
      661 tccgcgcgac tgacgatggc actggtgagc tcgtcgccga ctacgccgcc tggctggagg
      721 agaacccgcc cgaggaggcc ggctgggttc agccgccaga catccccgaa gagttcccga
      781 acccgggcaa cccctacgcg atggctttcg tgggtgatga gctgtggatg gtcgatgcgg
      841 tcaacggcct cgtggtgtcg atgggtctgg atggcacgat cgagctggtc gccgatctct
      901 cgcggagaca tccggtgccg acgggcatcg cgccggcccc ggagggtggc gcgtatgtcg
      961 gcacgctgac cgccgtgcca tatcctgacg aggtcgcgaa ggtcgtccac atcgcacctg
     1021 acgggatggt caccgacgcc tggacggggc tgacgatggt caccgccgtg gccgtcgatg
     1081 ccagcgggac actctatgcc gtggagatgt cgacagacaa catggaggag ccgccgttcg
     1141 tggtgccaga cagcggtcgg gtagtgcgcc agaccggacc agccagtctt gagcccgtgg
     1201 cggaagacct gctcttcccg atcgcgctca gcgttgggcc ggacgatgcg ctctacgttg
     1261 ccctgccggc agtcggagcc gataccggta gtggaatgat cgtgaggctg tcggacagtg
     1321 atggccatcc gacgatggac gagacgtcct gcgcgccgct ggccgagacg gtgaacgcgc
     1381 ctcaaggccc ggtggaggcg acaccggcaa cctagcgccc actcgctgat ggagcgctcc
     1441 ccggcaccct gccggggagc gctttctcgt gctcgggtcc gttgtctcgg tcgggcagca
     1501 cagccatgag attcgtgtcg gagctccctg ctcatcaggg aggtgtcggg taccataatg
     1561 cccagcaacg cagccgccgc ccgggccact ggagtgcgtt cgtcatatgc cgggcagcag
     1621 aggggaccag aggatcggag cattatggat cgacgcatga agtgggcgag acggctggcc
     1681 gtgatcaccg ccgtgggcat gtttctcgtc ctgctgatgg gcgcgaatgt gaccgcaacc
     1741 ggctccggcg aagggtgcgg tcgctcatgg cccctctgca acggcagtgt gctgcccgcc
     1801 cagacgctcg agtccttcat tgaatacagc caccgcattg tgaccagcat cgaggggatt
     1861 ttcgtggcgg cgacctcgat cgtcgcctgg ccgctgcgca agcggttccc gcagttcaag
     1921 gcgctggtgc ccgccgttgc cggcacgctg atcctgcagt cgctgatggg cgccgccgcc
     1981 gtgatgtggc cccagacacc ggaggtgatg gcgagtcact tcggcatctc gctcgtttgt
     2041 ctcgccgcgg cggcgctgat cgcacggatc gtgaccgagg cgcgcgacaa cgacgagcac
     2101 ctcgaggtcc ggcgcatcac cgcgccgacc ggctaccgcc tgctgatggg cgtcaccttc
     2161 atcttctcga tcgtggtggc ctactcgggc gcctacgtgc gccacaccgg tgccgagttg
     2221 gcctgcacca gctggcccac ctgcaatggc gagctgatcc cggagttcgg cgggctggcg
     2281 ggcaaccaga cgctgcatcg gctggcggcc ctgggcatca gcctgatgat tgtcacgctc
     2341 ctcgtctggt cgtatcgctt ccgggccgag cgggaggacc tgcaccggaa cgcactgctg
     2401 gccttcatcc tcgtcatcgc gcagtcgatc gccggggcga tcgtcgtctt ctctggcctg
     2461 cagctgctgg cagccctcac gcacgcggcg ttgatggcgc tgctcttcgt ggtgctcagc
     2521 gacggcctgc ggctggtgct gccaccaccc gagcggcaga ttgatgtcga gacggtgcag
     2581 tgcgggtcgg tcccgcgcat ggccaccggc gactgacggc tgggatcacc ggacacgatc
     2641 cattttgtag cgtcgcctcc tgtgggcgac gcttttcatt gagcgggttg gcagacattg
     2701 cggtccgagt gctgaagacg gatcaacata gagttgaccc gtacgggcgg actccatgtc
     2761 cgccctgatc tggtgtgcaa agactattgc gaacctgctt ggtgtgactt ttcggatggg
     2821 ttgtccgtca cgtcatcgtt caagatgctt caggcatcgc aggatcgtgc cgcgatcccc
     2881 gaaactcccg gccttggtca cgatcggtac ctcggggagc aagcgggagc gcaggatgcc
     2941 ccatggtatt gctgcctgca cttcgccgcc cagcatgatc tcggttgctt tcagcctggc
     3001 aagcacaccc gccgccacat cgcctccggt gagcaccaac ccgccgacca agtggcgctc
     3061 ggccagagcc gcgacaatcg ccgccagttg ccggacgacg aactccggtc ccttcgggga
     3121 cgccgcgagg cccgtggtgg tgagcgccac gaaacggctc tcgaaaagat gaggggcgag
     3181 tgcggcgact gtagtatcca ccggcgccgt tccgtcgagc atctcctgcg atggatggac
     3241 caggggcacg cccgcctcga ccagcgccgc gacctgtgcc gcggcggatg gatgcctgct
     3301 gccggcgacg acgaggatgg ggccggtgcg gcagtcgggc gtcgccgcac gtcgcgcgga
     3361 taccggggca ggcagggtgg tggcgagctg ccgggcgagg ccggccgagc ctgccatgac
     3421 ccggattcca ttgtcgagtg cagcgccggc gatcgtcatc aggtcacgat ccgtctccgc
     3481 gtcagcgacc aggatgccct cctcgagccc ctcgatggca tgtgcgacat gcccggtgcc
     3541 gtggcgaaca gtcgcgagcg tgatcggctg gatcgctacc tgccgctcgc agccgaacac
     3601 cgagatcagg tcggccccgc tggcgggaac gccgaatggc gtctggtcga gcggaattcc
     3661 gtcgacgagc tgacgcgacc cagcggtcgt ccgaccctgc gccgggagcg ccggcgctac
     3721 gagcgctcgc cgctcgccca actcccgcat caccgccagc agctcctcac aggggtggcc
     3781 gcggagtgcc gagtcgatct tcttgtagac ccaccgcggt ggttgccttg gtggcgggca
     3841 ccgaagaccg cgcaccgcac ggcgattgac ctccgcggcg tccgtcgtgt ccagccctcg
     3901 gctgtcggtc gaccgaacga tgacatcggc gtcctcgcta cgctcgttgc cgaacaggat
     3961 cacggtacgc agaccagcgg tcgcgaacgc gccaccggcg tcgccggcac cggtgaggtc
     4021 gtcagcgatg acggcgagca gtgtcaccgt ctgctgcctc tgctcggggc ttcctgcgat
     4081 gccgaacgtg atgcggccag ttgcaccgca acttctatgg ctgccagcat gctggactcg
     4141 ctggccttgc cggtgccggc gatatcgaag gccgtgccgt ggtccaccga ggtccgcagg
     4201 atcggcaagc cgatgctgac gttgacccca gagtcgaacg cgagcagctt catcgggatg
     4261 tggccctggt catgatacat cgccacgacg atgtcccact cccccttcgt ggcgcggaga
     4321 aagacagtgt ccggcggctg cggatcgctg acg
//
LOCUS       DASYWL010000266         4232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_426502, whole genome shotgun sequence.
ACCESSION   DASYWL010000266 DASYWL010000000
VERSION     DASYWL010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426502"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..897)
                     /locus_tag="VGR22_09570"
     CDS             complement(34..897)
                     /locus_tag="VGR22_09570"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2128853.1"
                     /translation="MSTHTVSAPTTTPGSHVRAPDLDFVRRSKPASVIADALVLARRH
                     LIRIPRQLDWLIGVTIMPIMFLLLFRYIFADTVQATLPPGVNAVNYLVAGIIVQGLVF
                     GSMNTGLGLATDSKEGLMDRFASLPMSRSAVVLGRILADMGIAVFTTTITILVGLLVG
                     FRPTTDVLDWVAAIGLMLLMAFVLAWLGAIIGLWLKTVEAVNSIGFSLIFPLTFVSST
                     FINPNFLPSWLQGFAKNQPFTLLLDATRGLLTGYPEVGNKGWMVTLYLLIALVVFVPV
                     ALRMYERRISG"
     gene            complement(894..1910)
                     /locus_tag="VGR22_09575"
     CDS             complement(894..1910)
                     /locus_tag="VGR22_09575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2128854.1"
                     /translation="MNRSRTGQQDAAIIAEGLVKTFGKTRALDGIDLHVPRGTVFGLL
                     GPNGAGKTTAVRILATLLKPDAGRAVVAGVDVVAHPDEARSKFGLTGQYAAVDEVLTG
                     RENLIMFGQLYRLSQRSARKRADELLQRFDLTDAANCSVKTYSGGMRRRLDLAASLIV
                     SPPVIFLDEPTTGLDPRSRNAMWEVIEDLVNDGITVLLTTQYLEEADKLADEIAVVDH
                     GRVIAEGTADQLKSQLGGERLAIGVASGTDLALAERTLLAIAGTSGDVDGEVQIDTVS
                     NQLTVPFAAGAQGLPEAVRQLDAAGLELTDLALRRPTLDDVFLALTGHATEENDDAAE
                     EHAA"
     regulatory      2134..2247
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2238..3059
                     /locus_tag="VGR22_09580"
     CDS             2238..3059
                     /locus_tag="VGR22_09580"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2128855.1"
                     /translation="MPDFHRTSTPRVELRDIHATYVEDGVTLEALRDISFNVHDAEFV
                     ALVGPSGSGKSTLLDIIAGLYAPSGGEVLLDGAALSTPERLGRSSYMHQRDLLLPWRS
                     ALDNAAVALEIHGVPKREARRRARERFPEFGLEGFEDAWPAQLSGGMRQRVAFLRTVL
                     AECPLLLLDEPFGALDALNRVVLQTWLLELWEKERQAVLFVTHDVDEAVYMADRVVVL
                     TARPGVITHIEDITLPRPRTRDVLTSECFHQHRVALLEALGLLETPTLQPIGDES"
     gene            3056..3829
                     /locus_tag="VGR22_09585"
     CDS             3056..3829
                     /locus_tag="VGR22_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2128856.1"
                     /translation="MIALTRRWSLPLLLLALFFVAWEIAVRLSDTPAWMLPRPSAIWE
                     AFRDNQALLWRHTRVTLLEVLFGFGVALTAGVTIGIAIDSSITLERALYPLIIASQTI
                     PMVVLAPLLLIWFGYGLTPKIIVAALIAFFPIAVSTVDGLRAADREMMAMLRSLGASR
                     WTRFRMAKLPAALPSIFSGARIGIAVSVIAAVFGELVGASEGLGYLMQRDSSQFLTAR
                     VFASIFILSLMGVGLFGLIGVLERLLLPWRRYVTDRPNG"
BASE COUNT          718 a         1396 c         1400 g          718 t
ORIGIN      
        1 tctcccagcc actcggcagc tctcctgagc cggctagccg gagatccggc gctcgtacat
       61 ccgcagcgcc accggcacga agacgacgag ggcgatcagg aggtaaagcg tcaccatcca
      121 gcccttgttg ccgacctccg ggtacccggt gagaagtccg cgagtcgcat ccagcagcaa
      181 ggtgaacggc tggttcttgg cgaatccctg cagccagctg ggcaggaaat tcggattgat
      241 gaaagtgctg ctgacgaagg taagcgggaa gatcagcgag aaaccaatcg agttcacggc
      301 ctccaccgtc ttcagccaca gcccgataat ggcgccgagc caagccagca cgaaggccat
      361 caacagcatc aggccgatgg ccgcgaccca gtcgagcacg tccgtggtcg gccggaaccc
      421 gaccagcagg ccgacgagga tcgtgatcgt ggtggtgaat accgcgatcc ccatgtccgc
      481 cagaatgcgt cccagcacca ccgccgagcg tgacatgggc agcgacgcga accggtccat
      541 caggccttcc ttggaatccg tggccaaccc gagtccggta ttcatgctgc cgaagacgag
      601 gccctgaacg atgatgccgg cgaccagata gttcaccgcg ttcacgccgg gcggcaacgt
      661 cgcctgtacg gtgtcggcaa agatgtaccg gaacagcagg agaaacatga tcggcatgat
      721 cgtgactccg atcagccagt cgagctgccg cggaatgcgg atcaggtgac gcctggccag
      781 cacaagcgcg tcggcaatga cgctggccgg cttgcttcga cggacgaaat cgagatctgg
      841 agccctgacg tgggagcccg gtgtcgtggt cggcgcagaa actgtgtggg tgctcatgcg
      901 gcatgctcct cggcagcgtc atcattctct tcagtcgcgt gcccggtcag ggccaggaag
      961 acatcatcga gggtggggcg acgcagtgcc aaatcggtca actccagacc cgccgcgtcg
     1021 agctggcgta cggcctccgg cagcccctga gctccagccg cgaacggaac ggtgagctga
     1081 ttggagacgg tgtcgatctg aacctcgccg tccacgtcgc cggaggtgcc ggcgattgcg
     1141 agcagcgtgc gctccgccag ggcgagatct gtaccgctgg cgacaccgat ggccagccgc
     1201 tcgccaccga gctgtgactt gagctgatcg gcggtgccct cggcgatcac tcgaccgtgg
     1261 tccacaaccg caatctcatc agccagcttg tcggcctcct cgagatactg ggtcgtcaac
     1321 agcacggtga tgccgtcgtt gacgagatcc tcgatcacct cccacatcgc gttgcggctg
     1381 cgcggatcga gaccggtcgt cggctcgtcg aggaagatca ccggaggcga cacgatcagg
     1441 ctggcggcga ggtcgagccg ccggcgcatg ccgcccgagt acgtcttgac ggaacagttg
     1501 gcggcgtcgg tgaggtcgaa gcgctgcagg agttcgtcgg cgcgcttccg ggccgagcgc
     1561 tgcgacagcc ggtagagctg gccgaacatg atcaggttct cacggccggt cagcacctcg
     1621 tcgacggcag catactgccc ggtgagcccg aacttcgagc gcgcttcgtc gggatgagcg
     1681 acgacgtcga cacccgccac cacggcacgg ccggcatccg gcttcaacag cgtcgcgagg
     1741 atacgcacag cggtcgtctt gccagcgccg ttcggcccta gcaggccgaa cactgtccct
     1801 cgtggcacgt gcaggtcgat cccgtcaagc gcccgggtct tgccaaaggt cttgacgagc
     1861 ccctcggcga tgatcgcggc gtcctgctgg ccggtacgtg atctgttcat acgctcccct
     1921 tcggcatgtc cggacactta ctccggtggc ggacaatccg cgggtggatt gttgcatcgg
     1981 cgtgcccaag agtcaacccc cggcaatgct ggagggaccg ggcggtacat ccatcctcac
     2041 gtgattctgg gggctcaccg gtatcccggc ttatccggtc ttgcgaacgg ccgcgctata
     2101 ctcttccgaa ctccccgcgt cgcccgacgc gggtgacacc gctacggggg ctcggggcag
     2161 ccgggctgag agggcgaccg catccgtcgc cgaccgtttg gacctgatcc gggtaatgcc
     2221 ggcgcaggaa ggcaccgatg cccgatttcc atcgcaccag cacgccacgt gtcgagctgc
     2281 gcgacatcca cgcgacctat gttgaggatg gcgtcacgct ggaggcgctg cgcgacatca
     2341 gcttcaatgt tcacgacgcc gagttcgtcg cgctcgtcgg gccatccggc tccgggaaga
     2401 gcacattgct cgacatcatt gccgggctct acgcgccctc cggtggcgag gtgctgctcg
     2461 acggcgcggc gctctccacc cccgagcggc tcgggcgctc ctcctacatg caccagcgtg
     2521 acctgctgct gccgtggcgg tcggcgctcg acaacgcggc ggtcgcactc gagatccacg
     2581 gcgtgccgaa gcgcgaggcg cgacgccgcg cccgcgagcg cttccccgag ttcggcctag
     2641 agggattcga ggacgcctgg ccggcacagc tctccggcgg catgcggcag cgtgttgcct
     2701 tcctgcgaac ggttctcgcc gagtgccctc tgcttctgct cgacgagccg ttcggcgcgc
     2761 tcgatgcgct caaccgcgtg gtgctgcaga cctggctgct ggagctctgg gagaaggaac
     2821 ggcaggcggt gctcttcgtg acgcacgacg tcgacgaagc ggtctacatg gccgatcgcg
     2881 tcgtggtgct gacggcgcgc ccgggcgtga tcacccacat cgaggacatt accctgcccc
     2941 ggccccgcac gcgggacgtg ctcaccagcg agtgtttcca ccagcaccga gtggcgctgc
     3001 tggaggcgct ggggttgctg gagaccccga cactgcaacc gatcggggat gagtcgtgat
     3061 cgcgctcacg cggcgctggt cgctgcccct gctgctgctc gccctcttct tcgtggcctg
     3121 ggagatcgcg gtgcggctct ccgacacccc ggcgtggatg ctgccgaggc cctctgccat
     3181 ctgggaggcg ttccgcgaca accaggcgct gctctggcgg cacacccgcg tgaccctgct
     3241 tgaggtgctg ttcgggtttg gggtcgccct gacggcgggc gtgacgatcg ggatcgcaat
     3301 cgacagctca atcacgctgg agcgggcact ctacccgctg atcatcgcca gccagacgat
     3361 cccgatggtg gtactcgcgc cgctgttgct gatctggttc ggctacggcc tgacgccgaa
     3421 gatcatcgtc gcggcgctga tcgccttctt ccccatcgca gtcagcacgg tcgacggact
     3481 gcgggcagcc gaccgggaga tgatggcgat gctgcgctcg ctcggagcga gccgttggac
     3541 gcggttccgg atggcgaagc taccggcggc gctgccctcg atattctccg gcgcacggat
     3601 cgggatcgcg gtgagcgtga tcgcggcggt cttcggagag ctggtcgggg cgagcgaggg
     3661 gctgggctac ctgatgcagc gggacagctc gcagttcctg acggcgcggg tcttcgcctc
     3721 gatcttcatt ctctcgctga tgggcgtcgg gctcttcgga ctgatcggcg tcctggagcg
     3781 cctgctgctg ccatggcgcc gctacgtcac cgaccgccca aacgggtagc tggcagggga
     3841 gaacttggcc gcgcttgttg atgacgagga gaggaatgcg tccgcagtca atagcctgac
     3901 cttgccctga cgctgagtga agcgaagggg aatggatcct cccgcacaac cgtgacgcca
     3961 cgtaatggga cgacattggc tgcgctgcac ggtggctcgc ctgcgccctg gtggcgcagc
     4021 caagtcgccc cctacggaaa cctgaggaga catcgtaacc atgagtgaca cagcgactat
     4081 gtccgcaccc gaacgattcc ggcaggccgc cgacgcgatc tggcaggcac agcacgacca
     4141 cccgttcgtg cgcggcatcg gcgacggctc gctcgacccg gagaagttcg gctactggct
     4201 ccggcaggac tacctcttcc tggtcgaata ca
//
LOCUS       DASYWL010000267         1787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_557775, whole genome shotgun sequence.
ACCESSION   DASYWL010000267 DASYWL010000000
VERSION     DASYWL010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557775"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..957
                     /locus_tag="VGR22_09590"
     CDS             262..957
                     /locus_tag="VGR22_09590"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV2128857.1"
                     /translation="METAHRDFKGLLYDQFARVTKAVANPHRLELIDLLAQGERRVDE
                     LAREAGLTMANASQHLQTLRQGGLVESRRDGTAIYYRLADEGVFRLWQAIREVGEARL
                     AEIDRLTRTYLHDRTQLEAIDVTTLERRLAEDDVVVLDVRPDLEYRAAHIAGARSIPV
                     TELASRLDEIPRDREIVAYCRGPYCVFSDEAVALLREHGYQARRLDIGLPDWHAAGMP
                     TESSDSPSEGRIS"
     gene            954..>1787
                     /locus_tag="VGR22_09595"
     CDS             954..>1787
                     /locus_tag="VGR22_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV2128858.1"
                     /translation="MNKSDTRTTVDREELRRMLSTGQPVTVLDVRNDADRAEWSIPGS
                     THVDAYAALKTGDTTPLDNLDLPKDRPVVTICGAGKTSKLAAERLRERGYDAHSLAGG
                     MRAWSLAWNTAEVPVPDSPTTVLQVRRTGKGCLSYLIGSEGEAVVVDAALDPEVFISL
                     AEERGWRIAHVLDTHIHADHLSRSRALAARTGATLHLPRQGRIAFPFESLEDGDAITV
                     GRATLSTLATPGHTMESMAYLLDGTALLTGDTLFLSAVGRPDLEASADEFRTKAHLLY
                     RS"
BASE COUNT          323 a          621 c          573 g          270 t
ORIGIN      
        1 tggcacatcc aagtcgagta ctcgggagtg atcccataca gccaacacgg gctaaagagt
       61 agcagtgaac gtcccccagc taatcccggc aaacggctcg cttccacgag cgggatgccc
      121 cgacaaccac cacgcaaatg aggggcccga ggaccacgat ccgtgtcttc cctctcacaa
      181 acgctgtggc agggcgcctg tcgagcccct cattgcggag agcgacttga cacacgcgaa
      241 tattcaagta ttatattgat catggaaacg gcgcaccgag acttcaaggg tctcctctat
      301 gaccagttcg cccgcgtgac caaggccgtc gccaacccgc accgcctgga gctcatcgac
      361 ctcctcgcgc agggcgagcg acgcgttgat gagctcgcgc gggaggcggg actgaccatg
      421 gccaacgcct cgcagcacct gcagacgctg cggcagggcg ggctcgtgga atcgcgccgg
      481 gacggcaccg cgatctacta tcggctcgcc gacgagggcg tgtttcgcct ttggcaggcg
      541 atccgcgagg taggcgaggc ccgcctggcc gagatcgacc gcctgacccg gacgtacttg
      601 cacgaccgca cccaactgga agcaatcgat gtcaccacgc tcgagcgccg cctggccgag
      661 gatgacgttg tcgtgctcga cgtccgccct gatctggagt accgcgccgc gcacatcgcc
      721 ggtgcccgtt ccatccccgt caccgagctc gcgagccggc tggatgagat cccgcgggat
      781 cgcgaaatcg tggcctactg ccggggaccc tactgcgtct tttccgacga ggcggtcgcg
      841 ctgctgcgcg agcacggcta tcaggcgcga cgcctcgata tcggcctgcc cgattggcac
      901 gccgccggga tgccgaccga gagcagcgat tcacccagcg aagggaggat ttcatgaaca
      961 agagcgatac gcgaaccaca gtcgatcgcg aggagctgcg ccgcatgctg agcacggggc
     1021 agcccgtgac ggtgcttgat gtgcgcaacg atgcggaccg ggccgagtgg tcgatccccg
     1081 gcagcaccca cgtcgatgcc tatgccgcgc tcaagaccgg cgacacgacg cccctcgata
     1141 acctcgacct ccccaaggac cggccggtcg tcaccatctg cggggccggc aagaccagca
     1201 agctcgcggc agagcgcctg cgcgagcggg gctacgatgc acactccctg gccggcggca
     1261 tgagggcctg gagtctggcc tggaacacgg ccgaggtgcc ggtccccgac agcccgacca
     1321 cggtgctgca ggtccggcgg accgggaagg gctgcctgtc ctacctcatc ggcagcgagg
     1381 gggaggccgt ggtcgtcgac gcggctctcg acccggaggt cttcatcagt ctcgccgagg
     1441 agcgggggtg gcggattgca cacgtcctcg atacccacat ccatgccgat cacctctccc
     1501 gctcgcgcgc gctggctgcc cggaccggag cgacgctcca cctgcccagg cagggtcgta
     1561 tcgccttccc gttcgagtcg cttgaggatg gtgacgcgat caccgttggg cgagccacgc
     1621 tgtccacgct ggccacgccg ggccacacga tggagtcgat ggcgtacctg ctcgacggga
     1681 cggcgctgct caccggcgac acgctcttcc tgagcgccgt gggccgcccg gatctggaag
     1741 ccagcgcgga tgaatttcgc accaaggcac acctcctcta ccgatca
//
LOCUS       DASYWL010000268         3554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_576542, whole genome shotgun sequence.
ACCESSION   DASYWL010000268 DASYWL010000000
VERSION     DASYWL010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576542"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..853)
                     /locus_tag="VGR22_09600"
     CDS             complement(23..853)
                     /locus_tag="VGR22_09600"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEV2128859.1"
                     /translation="MQLAYQTNTWGGVVGHPAGVTSVKDLYYLANGSTEEALTDIAGV
                     GYQGFELFDGNLMQYAGREDDLRGLMERLRLELVGVYSGANFIYPEILGEELSKIEEV
                     AGLAARLGATHLVVGGGAIRASGTEDRDYQLLAKGLDQVVALADRVGLTASFHPHLGT
                     CAQSPEQIASVFEQTSIGFCPDTAHLEAGGGDPADLIRTYAGRIPYVHLKDYADGAFL
                     PLGQGRQDFPQIMSGLRAAGYDGWLTVELDAYAGHPADAAAVSKTFLDEMLAGRGAKQ
                     "
     gene            complement(858..2525)
                     /locus_tag="VGR22_09605"
     CDS             complement(858..2525)
                     /locus_tag="VGR22_09605"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEV2128860.1"
                     /translation="MSMVGKRKPERVDVCIIGAGASGGTAAKVLTEGGARVVALERGP
                     WRAHESFGGDELANVNRYNLWPDPLLNPRTYRETEQDEPRVELFCPVPQMVGGGTVHW
                     QGWLPRLTENDFRLRTIAGDVPGASLVDWPISYADLEPYYTKVEWTFGVSGQGGANKY
                     ESFRSKGYPCPPMPTTRYARKFYEGCRTLGYNAFPTPMAALSQPYNGRPATVLSAFSQ
                     QHGDPTGTRSSALSVWIPGALRTGRFEVRPDCYVREIVVDEHGRAARAVYQDADGDLV
                     EQEADVFILACGAVESARLMLLSRSGKYPNGLANGSDLVGRNATFHEYSAAVGVFDDP
                     IYAWAGGGYVAASSFEFYEHDDARGFVGGCHIAAAGVGIPLPINWSVPDRPLWGPQAK
                     QMDREWFNHTFAVGMVVHDMPQHESRVELDERVTDAWGLPVARITNTIHPNDLAMGRW
                     VIDRNAEILEAADAKQVYRVYPTRITGNCSHQHGTTRMGDDPDTSVLSKDCQAHEVDN
                     LYVVDGGPFPTATGANPTMTIMANAWRVADGILARRGAPATAVDSRR"
     gene            complement(2509..3180)
                     /locus_tag="VGR22_09610"
     CDS             complement(2509..3180)
                     /locus_tag="VGR22_09610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEV2128861.1"
                     /translation="MTSRPGTFFDEHQRRTIEAAMARIIPTDSEPGAREAGTIEFLDR
                     YLSGIDYVYAKPDGSGFERLDGRRAEAWRQRIDIVRAKYIEGVTELDRRSQVRFGEIF
                     VHLTGEQQDGILTEMELPATWTEHNLELAQVTDGYGAPVEPAMQQTSAEVEMEFFPLL
                     ALHTRQGFYADPIYGGNKDHVGWRLIGFEGPHSLADAHAGRYTSRPFFAEDQIDWEQG
                     DEYGG"
BASE COUNT          601 a         1240 c         1068 g          645 t
ORIGIN      
        1 ggctgaaggg ccggtgtgta ttctattgct tggctcccct ccccgccagc atctcgtcga
       61 gaaacgtctt gctgaccgct gccgcatcgg ccggatgccc tgcatatgcg tcgagctcaa
      121 ccgtgagcca gccatcgtac ccggcggccc gaagcccgct catgatctgt gggaagtcct
      181 ggcgtccctg ccctaatggc aagaaggcgc cgtctgcgta gtccttgaga tggacatagg
      241 ggatgcggcc ggcgtacgtc cggatgaggt cagccgggtc gccgcctcca gcctctaggt
      301 gtgcggtgtc tgggcagaac ccgatagatg tttgctcgaa cacgctcgca atctgctcgg
      361 gcgactgcgc acacgtaccg aggtgaggat ggaagcttgc ggtcaatccg acccgatcgg
      421 ccaatgcgac cacctgatcg agccccttcg ccagcagctg gtaatcgcgg tcctcggtcc
      481 ccgaggcacg gatcgcccca ccgccaacaa ccagatgcgt cgcccccaac cgggccgcga
      541 gcccggctac ctcctcgatc ttgctcagct cttcgcccag gatctcggga tagatgaaat
      601 ttgcacccga gtagacgccg accagctcga gccgcagccg ctccatcagc cctcgcaggt
      661 cgtcctcccg cccggcatac tgcatcaggt tgccgtcgaa gagctcgaac ccctgatacc
      721 ccaccccagc gatgtccgtg agcgcctctt ccgtggagcc gttggcgagg tagtacaggt
      781 ccttgacgct cgtgacaccg gcggggtgcc cgaccacgcc gccccacgtg ttcgtctggt
      841 aggccagttg cacgcgatta cctcctgctg tccacggccg tcgccggcgc cccccgccgc
      901 gcgagaatgc cgtcggcgac ccgccacgcg ttggccatga tcgtcatcgt cggattggcg
      961 cctgtcgcgg tgggaaacgg gccaccgtcc accacgtaga ggttgtcgac ctcgtgcgcc
     1021 tgacaatctt tgctcagcac agacgtgtcg ggatcgtcgc ccatccgcgt ggtgccgtgc
     1081 tggtgtgagc agttgccggt gatgcgcgtc gggtagacgc ggtacacctg cttggcgtcg
     1141 gccgcttcca gaatctcagc attgcggtcg atgacccagc ggcccatcgc cagatcattc
     1201 ggatggattg tgttcgtgat cctggcgacg ggcaaccccc acgcgtccgt cacccgctcg
     1261 tcgagctcga cgcggctctc gtgctgcggc atgtcgtgca cgaccatccc cacggcgaag
     1321 gtgtggttga accactcccg gtccatctgc tttgcttggg gcccccacag cggccgatcg
     1381 ggcacgctcc aattgatcgg cagcgggatg ccaacgcccg cggcggcgat gtggcagcct
     1441 ccgacgaagc cccgcgcgtc atcgtgctcg tagaactcga agctgctggc agcaacgtac
     1501 ccgccaccag cccaggcgta gatcgggtcg tcgaagacgc cgaccgcagc actgtactcg
     1561 tgaaacgtcg cgttccgccc gaccaggtcg ctcccgttgg ccagaccatt ggggtacttc
     1621 cccgagcgcg acagcagcat gagtcgcgcg gactcgacgg cgccgcaggc gaggatgaag
     1681 acgtccgcct cctgctcgac gaggtcgcca tcggcgtcct ggtacacggc cctcgcggcg
     1741 cggccgtgct catcgaccac gatctctcgc acgtagcagt cgggccgcac ctcgaaccgt
     1801 ccggtccgca acgcgcccgg gatccagacc gagagcgcgc tcgaacgcgt gccggtcggg
     1861 tcgccgtgct gctgcgagaa ggcgctcaac accgtcgccg gtcgaccgtt gtacggctgg
     1921 gagagcgccg ccatcggcgt cgggaacgcg ttgtagccga gggtgcggca gccttcgtaa
     1981 aattttcgtg cataccgagt ggtgggcatc ggtgggcagg ggtagccctt gctgcggaac
     2041 gactcatact tgttcgcgcc accctgaccg gaaacgccga acgtccattc gaccttggtg
     2101 tagtagggct ccaaatcggc gtagctgatc ggccagtcaa ccagcgaggc accgggcacg
     2161 tcgccggcga tggttcggag ccgaaaatcg ttctccgtga ggcgcggcag ccagccctgc
     2221 caatgcaccg tgccgccccc aaccatctgc ggcaccgggc aaaacagctc gacccgcggc
     2281 tcatcctgct cggtctcacg atacgtgcgg ggattcaaca acggatcagg ccagaggttg
     2341 tagcggttca cgttggcgag ctcgtcgcca ccgaagctct cgtgcgcgcg ccacggcccg
     2401 cgctcgagcg ccaccacgcg cgccccgccc tcggtgagca ccttcgccgc cgtgccccct
     2461 gaagcccccg caccgatgat gcagacgtca acgcgctccg gctttcgctt acccaccata
     2521 ctcatccccc tgctcccaat cgatctggtc ctcggcgaag aacggccgcg acgtgtacct
     2581 cccggcgtgc gcgtcggcca gcgaatgggg accctcgaag cctatcagcc gccatccgac
     2641 atggtccttg ttcccgccgt agatcggatc ggcatagaac ccttggcgcg tgtgcagtgc
     2701 caacagcggg aagaactcca tctcaacctc agcgctcgtc tgctgcatcg ccggttcgac
     2761 cggcgccccg tagccgtcag tgacctgggc cagctccaga ttgtgctcag tccacgtggc
     2821 gggtaactcc atctcggtga ggatgccgtc ctgctgctcg cccgtgaggt gcacgaagat
     2881 ctccccgaac cggacctggc tgcgacgatc gagctccgtg acaccttcga tgtatttcgc
     2941 gcgaacgata tcgatgcgct gtcgccaggc ctccgcacgc ctgccgtcga gccgttcgaa
     3001 tccgctccca tccggtttcg catagacata gtcgatcccg gacaggtagc gatcgagaaa
     3061 ctcaatcgtc ccggcctctc tggcaccggg ctcactgtcc gtcggaatga tgcgcgccat
     3121 cgcggcctcg attgtccgcc gctgatgctc atcaaaaaag gttcctggcc tgctcgtcat
     3181 cgacgcctcc cgccatggtg ccttcagccc gcgttacagc aatccgtagc ccggtcactc
     3241 ggtggttggc caagttcttc cggcggcacg cagatgccca gggggcttca ttgaatggga
     3301 cgatcattgc cattgatgat cagcacagat cgcccccagc cagatggcca taggtcgcaa
     3361 acgcaccgcc caccgaaccg ttgctgactg attcctcgtt gactcctaga ctccgtatcg
     3421 atctagtact cctaggctcc gtgtcgatct agtgtactcg gtcgaataat gggttgggag
     3481 gcgccggtcg accaattgat tgcccacgac cggcatggcg gtgaacgcca tcagtgaagc
     3541 cactgcgcac gcac
//
LOCUS       DASYWL010000269         6013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_576622, whole genome shotgun sequence.
ACCESSION   DASYWL010000269 DASYWL010000000
VERSION     DASYWL010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6013
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576622"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..419)
                     /locus_tag="VGR22_09615"
     CDS             complement(114..419)
                     /locus_tag="VGR22_09615"
                     /inference="COORDINATES: protein motif:HMM:NF021188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HEV2128862.1"
                     /translation="MPKMSMQIPHSLSKEEAQSRVQGMITNLKEQYGDRISDLNEEWH
                     GDTGQFSFRAMGYKLAGTLQVTESDVRVNGDIPWAAKPFQGTIEATIRERTERLLRP"
     gene            603..1769
                     /locus_tag="VGR22_09620"
     CDS             603..1769
                     /locus_tag="VGR22_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEV2128863.1"
                     /translation="MHATEAPSWLNAANLVEPVATITGGETVAIDTWSCQSLDGGAGE
                     ALGVWRVTGEATVDGVVTSFTVILKGWPAMTGPGNPTDWNWSQREVRAYTSGELEALP
                     GGIRAPRCLAEINRPDGSTWTWLSSMSDGALETWELEHFVLVARHLGQFNGAYLTGQP
                     MPDTPWLSQQWTRQWVALSDQAIIEFSELPSHPIVDRVLAPAVKAEFQRIWDERHAWL
                     DTIEALPQTFCHLDAFKRNIFLRPGPTGNLEPGLIDWGFTGTAAVGEEITPLVAASLY
                     FGEARGIQPQVLDVAVFDSYVQGLREAGWNGDETLVRTAYVGSAILRYGVGVMRFLPA
                     LLDEAAHPMLEHIFRLPLDDLAQLHGGLNLWLVDLADEFRHLQAALPPPLPDAA"
     gene            1919..3079
                     /locus_tag="VGR22_09625"
     CDS             1919..3079
                     /locus_tag="VGR22_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128864.1"
                     /translation="MVPPPLDWLNVDELHEPVGRMLGAPVDAIVGWSTTQMVGGSGEA
                     IGVWEVSGIAFAEGTPREWMIVLKGWGAGSATISPDAWEWPRREGCVYTSTLLDELPP
                     GLAAPQYYGRTERPDGTEWLWLERVFDTDRDPWPLERFAEVARQLGRFNGHWLATGTL
                     PSDPWLSRDWVMKWAERVAPEIGMLDRADGHPLVDRAFPAEVRRGYRRIWKERARLYS
                     ALSGLPKTVCHLDAFRRNVSRRETQTGSEMALLDWSFTGIAAVGEEMTPLIAASLYFG
                     DVPPSQAAALESVVLDAYVAGLDDSGWHGDPELARLGYLIATVLRYGVGCVPQMVRVL
                     SGEAKGVTGPDGKIPPLEQVAGRFGVLDEWLVERSNEVLARLAPSSPGRRIS"
     gene            3079..4341
                     /locus_tag="VGR22_09630"
     CDS             3079..4341
                     /locus_tag="VGR22_09630"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2128865.1"
                     /translation="MSGATPAGSRPRDHSAPEDRNPAGTWLVPVLTLSTFVTMLQAMA
                     LGPLLPDISEDLDTSVSLLGQIPAATMFLAALIGFVAGPLADRLGHHRALLGGLVAVA
                     VSSACMALAPQFALLLAAALIGSLGRAVVQPVAVVIAGDRFNGDRQRRAVSWVMAGVT
                     GAVIAGVPALTTVAEAFGWRAALAGLAGLSAALVPFVRLGLGSAEPPSAPGATLTRVF
                     SAYHPLLAHRPTLALIIATLLGSAGIWVMATYLGAFYDEQHGYTTRQIGWVYFVPGVT
                     LFLGSLAAGSRIGALPLRPIFVIARVVTGLAIAGVLALPIPAIMGVGLLAVQGLSTGL
                     SGVAVVLLLMRESPAGHATTLTLNTAALSLGTGLGSTLGGLLLSIGDFELLGLAALLL
                     SVASGALVWAVPVRAEHMSRVPSISRPT"
     gene            4356..5324
                     /locus_tag="VGR22_09635"
     CDS             4356..5324
                     /locus_tag="VGR22_09635"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV2128866.1"
                     /translation="MAEVAGTTKRPIEVGLFLIAADIPYGGCVPSWQDLVAVATRADV
                     LGFDSLWLADHLLADFGWESEPRISVRECWSLISALAAVTQRIQLGPLVSCTAYRNPA
                     MLAATADTVQEISKGRLFLGLGAGWHKPEFQSFGFPYDHRASRFEEAVQIISGLLGGE
                     RVTFEGKFYQARDCELRPRGQQPPKPPIVIGAKGPRMLELTARYADGWDSDFIRNPAD
                     YTQVREKLVQVDAACHSVGRDPATLHRSVTIQVNMPGHSRPEDHPFAAGRASSPPASG
                     TPEELAELLLAYAAEGVSRVQVSLDPSTVEAVEALAPVLDILDESA"
     gene            complement(5331..>6013)
                     /locus_tag="VGR22_09640"
     CDS             complement(5331..>6013)
                     /locus_tag="VGR22_09640"
                     /inference="COORDINATES: protein motif:HMM:NF015764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2128867.1"
                     /translation="HAAYLALALMVVFRLPNLAERRTAPIFEGLRAVLADRPVALLLV
                     VSYLLSIGAVIIGIYLGVRIEEIGGTARQVGLAFSVASASELPVIALGGWLLARLGSH
                     RLIAVAAVVFAIRFLAFSTIATPEWLLPIQVLHGLSYAAFTLASITLIHQLVRRQYAA
                     TAQALLTAVSLGFGSITGSFVGGILLDVIGTEGLFLVAAALMVIALLVLVAGDRLVGL
                     TSRARDAT"
BASE COUNT          963 a         2013 c         1954 g         1083 t
ORIGIN      
        1 tccttcgact ccggcgcttc gggcctccgc tcaggatgac ggtgcaagtt gggtaacgac
       61 taacccgccc gcagaggggc gggtctcccg acaagacgtt tgctgtgttc gcgctatggt
      121 cgcagcagcc gctcggtgcg ctcgcggatg gtcgcctcga tcgtcccttg gaacggcttg
      181 gccgcccacg gaatgtcgcc attgacccga acgtcgcttt cggtcacctg cagcgtcccg
      241 gcgagcttgt agcccatcgc ccggaacgag aactggccgg tgtcgccgtg ccactcctcg
      301 ttcaggtcac tgatcctgtc gccgtactgc tccttcaggt tggtgatcat cccctgcacg
      361 cggctctgcg cctcctcctt cgagaggctg tgcgggattt gcatcgacat cttcggcatc
      421 tcgcgctcct tgggtgggtc gtggccgcgt cggtcggccc tcgccggtta taccccatcg
      481 gcagcagccc aggaaatgca ccagtgattg acgccgccgg ctcgcggatg tacgctgcct
      541 gcagggactc caaatatgtc aatatctacg ggcaatgccg gcaccttcga aaggaaagac
      601 ccatgcacgc caccgaggcg ccctcctggt tgaacgccgc gaatctcgtc gagcctgtcg
      661 cgaccatcac cggcggggaa acggtcgcca tcgatacatg gtcgtgccag agcctagatg
      721 gcggcgcggg agaggcgctc ggcgtctggc gggtgaccgg cgaggcgaca gtcgatgggg
      781 tcgtcacgtc gttcacggtc atcctcaagg gctggcccgc catgacgggg cctgggaacc
      841 ccaccgactg gaattggtca cagcgcgaag tgcgcgccta cacctcgggc gagttggagg
      901 cgctcccggg tggaatccgt gcgccgcgtt gtctcgccga gatcaatcgg cccgacggct
      961 cgacctggac atggctcagc tcgatgtcgg acggcgcgtt ggagacctgg gagctggaac
     1021 acttcgtcct cgtcgcccgg catctcggac agttcaacgg cgcgtacctc accggacagc
     1081 caatgcccga caccccctgg ctgagccaac agtggacccg ccagtgggtc gcactctccg
     1141 accaggcgat catcgagttc agcgaactcc cgagccatcc gatcgtcgat cgtgtcctcg
     1201 cgcccgcggt caaggccgag tttcagcgca tctgggacga gcggcatgcc tggctcgaca
     1261 cgatcgaggc gctgccgcaa accttctgcc accttgacgc cttcaagcgg aacatcttcc
     1321 tgcgccccgg tccgaccggc aatctggagc ccggcctgat tgactgggga ttcaccggca
     1381 ccgccgccgt cggcgaggag attaccccac tcgtggcggc gagtctctac ttcggcgagg
     1441 cccgcggtat ccagccgcag gtgctcgacg tcgccgtgtt tgacagctac gtgcagggat
     1501 tgcgggaggc cggctggaac ggcgacgaaa cgctcgtgcg aaccgcctac gtcgggtccg
     1561 ccattctgcg gtatggcgtc ggcgtgatgc gattcctgcc ggcgctgctg gacgaggcgg
     1621 cccaccccat gctcgagcac atcttccgac tgccgctgga tgacctcgcc caactccatg
     1681 gagggctcaa cctctggctc gtcgacctgg cggatgagtt ccggcatctg caggccgcac
     1741 tgcccccgcc cctgccggat gcggcatgag gctggccgcc gcctcaccgc ccgggcacgc
     1801 tcggacattg ccagttgcac acgctcaggt tgaacctgtc ctgcgcgggg cgtatggtgc
     1861 gacgaatatc ttccgctgcc cctggttccc ggaaccatcc aagcgacgga gtgccgtcat
     1921 ggtcccaccg cccctcgact ggctcaacgt tgacgaattg cacgaaccgg taggccggat
     1981 gctcggcgct ccggtcgatg ccatcgttgg gtggagcacg acacaaatgg tcgggggctc
     2041 cggcgaagcg atcggggtct gggaggtatc gggcattgcc tttgccgagg ggacgccacg
     2101 cgaatggatg atcgtcctca agggttgggg cgccggatcg gccacgatct cgcctgatgc
     2161 gtgggaatgg ccccgacggg aggggtgcgt ctatacctcc acgttgctcg acgagctccc
     2221 accaggtctc gctgctccgc aatattatgg tcgcaccgag cggccggacg gcactgagtg
     2281 gctctggctg gagcgcgtgt ttgatacgga tcgcgacccg tggccactcg agcgctttgc
     2341 cgaggtcgcc cgccagctcg gccggttcaa tggccactgg ctggccaccg gcaccctccc
     2401 cagcgatccc tggctgagcc gtgactgggt catgaagtgg gctgagcgag ttgcacctga
     2461 aatcggcatg ctcgaccgcg ccgatgggca cccgctcgtc gaccgcgcgt ttcccgccga
     2521 ggtgcgccgc gggtatcggc gcatctggaa ggagcgagcc cggctctact cggcgctgtc
     2581 cggcttgccg aaaaccgtct gccatctgga tgcgttccgc cgaaacgtct cgcgccgcga
     2641 aacccaaacc ggctcggaaa tggccctgct cgactggtcc ttcaccggga tcgccgccgt
     2701 cggcgaggag atgaccccac tgatcgccgc cagcctctac ttcggtgatg tcccacctag
     2761 ccaggcggcg gcgctggagt ccgtcgtgct cgacgcctat gtcgccgggc tggatgacag
     2821 cgggtggcac ggtgacccgg agctcgcgcg gctcggctac ctgatcgcga cggtgcttcg
     2881 ctatggggtc gggtgtgtac cgcagatggt gcgtgtgctc agcggggagg ccaagggcgt
     2941 gacaggccca gacggcaaga tcccgccatt ggaacaggtc gccgggcgat tcggcgtgct
     3001 ggacgagtgg ctggtggagc ggagcaatga ggtgctcgcc cgactcgctc cctcgtcccc
     3061 gggcaggaga atatcctgat gtccggcgca acacctgctg ggagccgccc gcgtgaccac
     3121 tccgcgccgg aggatcgcaa ccctgccggt acgtggctcg tgcccgtcct gaccttgtcg
     3181 accttcgtga ccatgctcca ggcgatggca ctcggccccc tgctgcctga catctcggag
     3241 gatctggaca cgagtgtctc gctgctcggg cagattcccg ccgcgacgat gttcctggcc
     3301 gcgctgatcg ggttcgttgc aggcccgctc gccgaccgtc tcgggcacca ccgcgccctg
     3361 cttggcggtc tcgtcgccgt ggccgtcagc tcggcgtgca tggccctggc gccccagttt
     3421 gcactgctgc tcgctgccgc cctcatcggg tcgctcgggc gtgccgtcgt acaacccgtc
     3481 gccgtcgtga tcgccggcga ccgcttcaac ggcgacaggc agcggcgcgc cgtcagctgg
     3541 gtgatggcgg gtgtcaccgg agcggtcatt gccggcgtcc cggcgctgac gaccgttgct
     3601 gaggcgttcg gctggcgcgc agcccttgcg ggactggccg ggctctccgc tgcgcttgtg
     3661 cccttcgttc gactcggtct cggttcagcc gagccaccct ccgcgcccgg tgcgacactc
     3721 acgcgcgtgt tttccgccta ccatcccctc ctggcacatc ggcccacact cgcgctgatc
     3781 atcgcgacgc tccttggtag cgcggggatc tgggtgatgg cgacctatct cggggccttc
     3841 tacgatgagc agcacggcta caccacccgc cagatcggct gggtctactt tgtgcccggt
     3901 gtcaccctgt ttctcggcag tctggctgcg ggtagtcgca tcggcgccct tccgctccgg
     3961 ccgatcttcg tgatcgccag agtggtcacc ggtctcgcca tcgccggtgt gctggcactc
     4021 cccatcccgg caatcatggg cgtcgggttg ctcgcggtgc agggcctctc aaccgggttg
     4081 tcgggggtgg cggtggtgct cctgctgatg cgggagtcac cggcgggcca cgccacgacg
     4141 ctgacactca acacagccgc actcagcctc ggcacagggc tcgggagcac cttgggtggg
     4201 ttgctgctct cgatcgggga tttcgagctg ctgggccttg ccgcgctgtt gctgtccgtg
     4261 gcatcgggag cgctcgtctg ggccgtccca gtgagggcag agcacatgtc gagagttccg
     4321 tcgatcagcc ggccgacgta ggtaggagat cagagatggc agaggtcgca ggcacgacga
     4381 aacgcccgat cgaggtcggg ctctttctca tcgctgccga tatcccctac ggggggtgtg
     4441 ttccctcctg gcaggatctg gtggctgtcg ccacccgagc agatgtgctc ggcttcgatt
     4501 cgctctggct cgctgatcac ctgcttgcag acttcggttg ggaatccgag ccgcggatca
     4561 gtgtgcggga gtgctggtca ctcatctcgg ccctggcggc ggtaacccag cgcatccagc
     4621 ttgggcccct tgtcagctgc acggcgtatc gcaaccccgc gatgctcgct gccacggcgg
     4681 atacagtcca ggagatcagc aaaggccgac tgttcctcgg cttgggcgcc gggtggcaca
     4741 agccagaatt ccaatccttc ggcttcccgt atgatcaccg cgcgagtcgc tttgaggagg
     4801 cggtccagat catctccggc ctgctgggcg gggagcgagt aacgttcgag ggcaagttct
     4861 accaggcgcg cgattgcgaa ctgcgtcctc gaggtcagca accgcccaaa ccaccgatcg
     4921 tgatcggagc gaagggcccc cgcatgctgg agttgaccgc tcgttacgcc gatgggtggg
     4981 attccgattt catcaggaac ccagccgact acacccaagt gcgcgagaag ttggtccagg
     5041 tggacgcagc ctgccactcc gtcggccgtg atcccgccac gcttcacagg agcgtaacga
     5101 tccaggtgaa tatgcctggg cacagtagac cagaggatca ccccttcgcg gcggggagag
     5161 catcgtcacc tcccgcgtct ggaaccccgg aggagttggc agagttgctg ctggcctatg
     5221 ccgccgaggg tgtcagccga gtgcaggtct cgctcgatcc gagcaccgtc gaggccgtcg
     5281 aagcgctcgc cccggtcctc gatatccttg atgaaagcgc ttgagttgat tcatgttgcg
     5341 tccctcgcgc ggctggtcaa tcccaccagg cgatcgccgg ccaccagcac gaggagcgca
     5401 atcaccatca gtgccgctgc gacgagaaag agtccctcgg tgccgatcac gtcgagcaga
     5461 atgcctccga cgaacgatcc cgtgatgctt ccgaatccca gcgacactgc cgtgagcagc
     5521 gcctgcgcgg tcgccgcata ttggcgtctg accagttggt gaatcaacgt tatcgaggca
     5581 agggtgaagg cagcgtacga aaggccatgc agcacctgga tcggcaacag ccactctggc
     5641 gtggcaatgg tactgaacgc caagaaccgg atggcgaaga ccacagccgc cacggcaatg
     5701 agccggtgcg acccaagcct agcaagaagc cagccaccga gcgcgatgac cggaagttcg
     5761 ctggcggatg ctaccgagaa tgccagcccg acctgccggg ccgtccctcc gatctcctcg
     5821 atgcgaacac cgagatagat gccgataatc accgcgccga tggagagcag atacgaaacc
     5881 acgagcagca gagcgaccgg tcgatccgcc agcacggccc gcagcccttc gaagatcggc
     5941 gccgtgcgac gctcagcgag gttcggaagt cggaacacca ccatcagcgc cagtgcgaga
     6001 tacgcggcat gcg
//
LOCUS       DASYWL010000270        11629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_426746, whole genome shotgun sequence.
ACCESSION   DASYWL010000270 DASYWL010000000
VERSION     DASYWL010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 11629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11629
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426746"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="VGR22_09645"
     CDS             <1..1108
                     /locus_tag="VGR22_09645"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEV2128868.1"
                     /translation="VRSAIDEAHAAGVRVLMITGDHPRTAARIASDLGINAPGDPTLV
                     GADLETLSDSELEEAVRTTDVYARVAPEHKLRIVDALQANDNIVSMTGDGVNDAPALK
                     SADIGVAMGITGTDVTKEAADMILADDNFATIIAAVREGRGIFANIRKFIRFLLSSNV
                     GEVLTMFLGVVLAGFLGLDQAGEAIAVPLLATQILWINLLTDTGPALAMGVDRPPDDV
                     MRRPPRPLTERVINREMWAGIIWIGIVMAVVTLFALDLRLPGGLVGDAGEIVTARTMA
                     FTTLVLAQLFNTFNARSDRASAFSHMFTNRWLWGAITLSLLLQVAVVQLEVLNDAFGT
                     TPLTLDEWAICAALASVVLWAEEARKLLKQMAGR"
     gene            complement(1105..3531)
                     /locus_tag="VGR22_09650"
     CDS             complement(1105..3531)
                     /locus_tag="VGR22_09650"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEV2128869.1"
                     /translation="MIERDVFEVDPWTLVEPRLRLDHLGQTESIFALSNGHIGLRGNL
                     DEGEPHVTPGTYLNGFFEHVPLPYAETGYGYPELGQSIIDVTNGKLLRLLVDDEPFDV
                     RYGTLRSHRRVLDLRAGVLRRELEWVSPAGKAVRVRSTRLVSFSQRAIAAIDYEVEPV
                     DEPARIVVQSSLIANEPVPEQAHDPRTAAALRAPLVGEFHWHHDLNVSLGHRTKLSNL
                     GMAAALDHVIEGPADTITESESETDLARVTISTDLAPGEPLRVTKLMAYGWSSVRSMT
                     ALRDQVDAALSGARRTGWDGLVAEQRKWLDRVWDHSDIEIDGNPRLQQAVRFAVFQVV
                     QAAARVEERAIPAKGLTGRGYDGHTFWDMESYVLAALTYSSPRAARQALLWRHATLDL
                     ARARARELRLAGAAFPWRTIRGEESSGYWPAGTAGFHINADIADAVHRYISASGDTEF
                     EQGPGLELLVETARLWRSLGHHDPEGRFRIDGVTGPDEYTALVDNNTYTNLMAARNLR
                     IAAGVAERHPEQAAELGVDDAEIASWWRAADNIRIPYDSELGVTMQSEGFTRYRKWDF
                     TNTGEDEYPLLLHYPYYLLYSSQVVKQADLVFALYTCSEHFPPDQKRRDFNYYEAITV
                     RDSSLSAAVQAIVAAEVGHLALAYDYLRETAYVDLWDLAENTADGLHLAALAGAAMVV
                     AAGFGGMRNYGESLTFSPRLPRQLERIAFRMLYRGRRLRVEITADEARYTLAEGEALD
                     LHHHGKPFRLATGATESLPMPWEPNVPAVVPPPGREACRQGIGANGIDGREAYRESHE
                     PSIEDRLLVR"
     gene            complement(3528..4325)
                     /locus_tag="VGR22_09655"
     CDS             complement(3528..4325)
                     /locus_tag="VGR22_09655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HEV2128870.1"
                     /translation="MASDGSTEQIGTSRRKGSLPAVPEHIAACLFDLDGVLTHTATVH
                     SAAWKEMFDAFLRERAEAGGEPFRPFEIATDYARYVDGRLRLDGVRGFLASRGIELPE
                     GTPDDPPETDSVHGLGTRKNTRVLEIIDERGVDIFPGSVRFVDVARDAGLRRAVVSAS
                     KNCQTVLEAAGIAHLFDVRVDGIYAAEHELPGKPAPDTFLAAAAELGVEPARCAVFED
                     AVAGVEAGQAGEFGWVVGVDRVGQAEALCAHGADTVVGDLEELLEER"
     gene            complement(4365..5279)
                     /locus_tag="VGR22_09660"
     CDS             complement(4365..5279)
                     /locus_tag="VGR22_09660"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEV2128871.1"
                     /translation="MRNLPFDQPGQFWRGNTHAHSTNSDGTMTPKEVVAVYRDRGYDF
                     VAVTDHFMERFDFPVTDTRDLRTSDFTTLIGAELHGQGLENGGLWHIIALGLPVDFDP
                     QRPGEDGAEIAARARAEGAFISLPHPQWTGVTPKDARKIEDFDAIEIHNEGHQNDSDR
                     GNGWFLADLMATAGYRFSCTAADDAHFNVRPDSFGGWICVKAEALEPEALLEAIKLGR
                     FYASTGPEIHNVAFTEDEIVVECSPAEVVMAGGHGSTCRYKRESGMTRAVFPIAPFGN
                     AFARITVIDRDGKKAWTSPLWLDELTIT"
     gene            complement(5333..6154)
                     /locus_tag="VGR22_09665"
     CDS             complement(5333..6154)
                     /locus_tag="VGR22_09665"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV2128872.1"
                     /translation="MNASGEQPRRVLITGSSGLIGRTLTDAWRRSGRFVPIGLARSEG
                     PYTDIIGDITDLDALVASMDGIDAVVHLAATSAVSSEWDAVRDSNLIGTYNVFEAARR
                     AGVNKVVFASSNHAVGTFETSNRPELYDLDDGRMIDHTVEIQPDSLYGVSKAYGEAMA
                     RFYVDHHGVSAVCLRIGSWTGADSPMTPQQLWDPAKDDNPEAIANRRRYRATWLSDRD
                     GVHLIECALATRKRWFLCYGISNNPRRFWDIEHAKRGIGFTPQDAAPEEILPGGW"
     gene            6288..7307
                     /locus_tag="VGR22_09670"
     CDS             6288..7307
                     /locus_tag="VGR22_09670"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEV2128873.1"
                     /translation="MPLIALARRLLPGLALAAGITLLAIGIAGLETLVFGYPLLEPLV
                     LALILGVVVRALWQPPAVFEPGIAFGAKQVLELAIVLIGVSLALGTLVDAGARLAVAI
                     LLLVSMTIALGVTLGRAAGLPVKLAVLIGVGNAICGNSAIAAVAPVIRARKQDVASAI
                     ALTAVLGIGVVLTLPLLVPLLAISDPEYGVVAGLSVYAVPQVLAATFPVSATAGQVAS
                     LVKLSRVLLLGPTVAILAILFRERDAEAGSGLTFSKLVPWFVIGFGIAVALRSAGLVP
                     EAVSDAARESSRVLTAVAMAGLGLSVDIRSVRETGSRVALVVLGLTLVLVTSAVAITL
                     VLDLP"
     gene            7428..7679
                     /locus_tag="VGR22_09675"
     CDS             7428..7679
                     /locus_tag="VGR22_09675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEV2128874.1"
                     /translation="MPTYVYTCDACEAVFDRERSVDERDEPLPCPVCDTPSRRKPVSP
                     KLLFKGDPAENRPYWHNHDGYSHSHAPRRGRHRTPEEDH"
     gene            complement(7960..9288)
                     /locus_tag="VGR22_09680"
     CDS             complement(7960..9288)
                     /locus_tag="VGR22_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEV2128875.1"
                     /translation="MRQDGESASTALGHDELVARHRESLFPSVGLFFDEPIELRSGEG
                     QLVRDADGREYLDFFGGIATVSSGHAIREINEPIKEQLDRIAHSSTLFLIRSQIELAE
                     RLREMTPERLNKVFFTNSGTEANEAAFLITTLNRNSNELLALRHSYHGRSFATINASG
                     QTPWRSSTLSPLHVHYIGNPYCYRCPWGKEYPSCDLFCAEDVEAVIQTTTSGNPAAFI
                     AEPIQGVGGFVTPPPEYFVRVGEILRRYGIPFISDEVQTAWGRIGVADFGFQAYGIEP
                     DVVVFAKGLANGIPIGGVIATDELANSIRSLSLSTFGGNPIATTAALANLNYIATNNL
                     RENARDIGGYMQDRLRELAERWPVIGDVRGLGLMIGVELVHDRQTKEPAADVVLRVQD
                     EAKRRGLIVGRGGLHANTLRICPPLIVSRSDVDAAIEILDRSFETVLDTA"
     gene            9468..9668
                     /locus_tag="VGR22_09685"
     CDS             9468..9668
                     /locus_tag="VGR22_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128876.1"
                     /translation="MILISATVMRASRRDRAMGAVGIAMGTLALIAYLARSDEALPEP
                     QAAPWVVESTTPDTPASPTIGP"
     gene            9682..9903
                     /locus_tag="VGR22_09690"
     CDS             9682..9903
                     /locus_tag="VGR22_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128877.1"
                     /translation="MSETTGAVVIIVGLAISAVGFLLMVRSQREVLGFAIIVVGFMLA
                     GTGMLTIEDTVVEERTPVTLPTSAATPAS"
     gene            complement(10207..>11629)
                     /locus_tag="VGR22_09695"
     CDS             complement(10207..>11629)
                     /locus_tag="VGR22_09695"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEV2128878.1"
                     /translation="LATGQLLGEHALYDVRSIGELQDRVRQAVAGVAPGTWVLGRGWD
                     ETLLAEGRMPTRHDLDAVAPDHPVVLQRVWNKLVVNTRALRELGIDATTPDPPVDVNY
                     AGGFDREEDGHPTGVFRDRAKELVLHGMPQPTEDDLVAALERACRAYNDVGLTAVVDP
                     GLPPHQLDAYMRAAELGVMSVRTDLLMATWGFVPATEEPELEDRIAGMAWRWPDRHPM
                     ARLTGVKLLPDGGIGDRTARVSEPYVGEPDNYGVWAVPEDELPERIRWVHAQGWPMDI
                     HTCGDEAQRVSVKAFADAQEANPRPELRHRVHHAYLPTPDTLERMARHRIPAVISTPF
                     IRSLGESFVQSLGEERAQRIMPFRTYLGHGVPLAGSSDSTVADHNPWVGMATALTRQT
                     VTGRVLGADQVLTPEEALALYTSWAAFAMGREGELGRLAPGYRADLAVLERDVFSEGV
                     DPAEIAATRPARVMLDGEWVVAG"
BASE COUNT         1908 a         3965 c         3754 g         2002 t
ORIGIN      
        1 ggtgcgttcc gccatcgatg aggctcacgc cgccggagtt cgcgtactca tgatcaccgg
       61 cgatcaccct cggaccgcgg cgcgcatcgc cagtgatctc gggatcaacg cgcccggtga
      121 cccgacactc gtcggggcgg acctcgaaac cctgagcgat tcggaactcg aagaggcggt
      181 gcgcacgact gatgtctacg cgagggtggc tccggagcac aagctccgga tcgtcgatgc
      241 gcttcaggcc aacgacaaca tcgtgtccat gaccggtgac ggcgtgaacg acgcgccagc
      301 gttgaaatcc gccgatatcg gtgtcgcgat gggcatcacg ggcacggacg tgacgaagga
      361 ggcggcggac atgatccttg ccgacgacaa cttcgccacg atcatcgccg ccgtgcggga
      421 ggggcgcggc atcttcgcga acatccgcaa gttcatccgg ttcctgcttt cgtcgaacgt
      481 cggcgaggtg ctcaccatgt tcctcggcgt tgtgctggcc gggttcctcg ggctcgatca
      541 ggccggtgag gcgatagcgg taccgctcct tgccacgcag atcctctgga tcaacctgct
      601 caccgacacc gggcccgcac tggcgatggg cgtcgaccgg ccaccagacg acgtcatgcg
      661 ccgaccgccg cgcccgctca cggagcgggt gatcaatcgc gagatgtggg cggggatcat
      721 ctggatcggg atcgtgatgg cagtggtcac cctgttcgca ctcgacctgc gcctgccggg
      781 cggcctcgtc ggtgatgccg gcgaaatcgt caccgccagg acgatggcct tcaccaccct
      841 ggtgctggcc cagctcttca acacgttcaa cgcccgctcc gaccgcgcca gtgcgttcag
      901 ccacatgttc accaaccgct ggctgtgggg tgccatcacg ctgtcactgc tgttgcaggt
      961 ggcagtggtg cagctcgagg tcctgaacga cgctttcggc acaacccctc tcactctgga
     1021 cgagtgggcg atctgcgccg ctctggcgag cgtggtgctg tgggcggagg aagccaggaa
     1081 gctgctcaag caaatggcgg gccgctagcg caccagcagc ctgtcttcga tcgatggctc
     1141 atgggactcg cgataggcct cccgcccatc gatcccgttc gcgccgatgc cctggcggca
     1201 cgcctcccgg ccgggcggcg gcaccactgc cgggacattc ggttcccaag gcatcggcag
     1261 ggactcagtc gctcctgtcg ccagccggaa cggcttgccg tgatgatgca ggtcgagcgc
     1321 ctcgccctcc gccagtgtgt atcgtgcttc gtcggcagtg atctccacgc ggagccggcg
     1381 tccccggtag agcatgcgaa atgcgattcg ctccaactgc ctgggaagtc gcggcgagaa
     1441 ggtcagcgac tcgccatagt tccgcatgcc accgaaaccg gccgcgacga ccatcgccgc
     1501 accggccagc gctgccaggt ggagcccgtc ggcagtgttc tccgccaggt cccacagatc
     1561 gacgtaggcg gtctcccgaa ggtagtcgta cgccagggcc agatgcccga cctcggcggc
     1621 cacgattgcc tggaccgcgg cggagagcga ggagtctcgc accgtgatgg cctcgtagta
     1681 gttgaagtcg cgccgcttct ggtccggtgg aaagtgctct gagcaggtgt agagcgcgaa
     1741 gaccagatcg gcctgcttga caacctggct ggagtagagc aggtagtagg ggtaatggag
     1801 cagcaacggg tactcatcct caccggtgtt ggtgaagtcc cacttgcgat acctggtgaa
     1861 cccctcggac tgcatcgtga cgccgagttc actgtcgtac gggatccgaa tattgtcagc
     1921 cgccctccac caggaggcga tctcggcgtc atcgaccccc agctcggccg cctgctcggg
     1981 atggcgctcg gccacgccgg ccgcgatacg aaggttccgg gcggccatca ggttggtata
     2041 ggtgttgttg tcgaccagcg cggtgtactc gtcaggcccg gtcacgccat cgatgcggaa
     2101 ccgtccctca ggatcatgat ggccgaggga gcgccaaagc cgcgccgttt ccaccagtag
     2161 ctcgaggccg ggtccctgct cgaactccgt gtcgccggag gcgctgatgt agcgatgcac
     2221 ggcatcggcg atgtcggcgt tgatgtggaa ccccgccgtg cccgccggcc agtatccgga
     2281 ggactcctcg ccgcggatgg tccgccacgg gaacgccgcg ccggcgaggc gcagctcacg
     2341 agcgcgtgca cgggcgaggt caagggtggc atgccgccag agcaacgcct ggcgagccgc
     2401 cctcggcgac gaataggtca acgccgccag cacgtacgac tccatgtccc agaatgtatg
     2461 gccgtcgtag ccgcgaccgg tcagcccctt ggcagggatc gcccgctcct cgacccgcgc
     2521 cgccgcctgc acgacctgga agaccgcaaa tcgcaccgcc tgctgcaggc gcggattgcc
     2581 gtcaatctcg atgtcggagt gatcccagac gcgatccagc cacttgcgct gctcggcaac
     2641 tagtccatcc cagccagtcc ggcgggcgcc cgaaagcgct gcgtccacct ggtcccgcag
     2701 cgcagtcatc gagcgcacac tcgaccagcc gtacgccatc agcttggtga ctcgtagcgg
     2761 ctcgccgggg gccaggtcgg tactgatcgt cactcgggcg aggtcggttt cgctctcgga
     2821 ttccgtgatg gtgtctgccg gcccttcgat cacatgatcg agcgcggctg ccatgccgag
     2881 attgctcagc ttggtacggt gcccgaggga gacgttcagg tcgtggtgcc agtggaactc
     2941 gcccacgagc ggggcgcgca gcgccgcggc ggtacgtggg tcgtgcgcct gctcgggaac
     3001 tggctcgttg gcgatgagac tcgactgcac cacgatacgg gccggctcgt cgaccggctc
     3061 gacctcatag tcgatggcgg cgatggcccg ctgcgaaaac gaaacgaggc gcgtggaccg
     3121 gacgcgaacc gccttgccag ccggcgagac ccattccaac tctcgccgga gcaccccggc
     3181 gcggaggtcc agcacgcgcc gatgggatcg caacgttccg tagcgcacat cgaacggctc
     3241 gtcgtcaacc agcagccgca ggagcttccc gttggtcacg tcgatgatcg actgacccag
     3301 ctccgggtag ccataccccg tctcggcgta gggcagcggc acgtgctcaa agaagccgtt
     3361 caggtaggtg ccgggtgtga cgtgcggctc gccctcgtcc aagttgccgc gcaacccgat
     3421 gtgcccgttc gagagggcga agatggactc ggtctggccg agatggtcca ggcgaagccg
     3481 tggctccacc agtgtccacg gatccacctc aaagacgtct cgctcgatca tcgctcctcc
     3541 agcaactcct cgagatcccc gaccacggta tcggcaccgt gcgcgcatag cgcctcggct
     3601 tgaccaacgc ggtcaacgcc aaccacccag ccgaactctc ctgcctggcc ggcttccacc
     3661 cctgcaaccg catcctcgaa gacagcgcac ctggccggct ccactccaag ctcggcggcc
     3721 gcagccagaa acgtgtcagg cgcgggcttt cccggcagct cgtgctcggc ggcatagatg
     3781 ccgtccaccc gcacgtcgaa caggtgtgca atcccggcgg cctccagcac cgtttggcag
     3841 ttcttgctcg ccgagacgac cgcgcgacgc agcccggcgt cccttgcaac gtcaacgaag
     3901 cgcaccgaac cgggaaagat gtcaacgccc cgctcgtcaa tgatctcgag gacgcgcgtg
     3961 ttcttgcggg tgccgaggcc gtgcacgctg tcggtctcgg gcggatcgtc gggcgtcccc
     4021 tccggcagct cgatgccacg ggatgccagg aagccgcgca cgccatcgag ccgcaggcgg
     4081 ccatcgacgt accgcgcgta gtccgtggcg atctcgaacg gccggaacgg ctcgccgccc
     4141 gcttcggccc gctcgcgcag gaaggcgtcg aacatctcct tccaggcggc cgagtggacc
     4201 gtcgccgtgt gcgtcagcac accgtccagg tcaaacaggc atgcggcgat gtgctccggc
     4261 accgccggca gcgacccctt ccgccgcgac gttccaatct gctccgtgct gccgtcactg
     4321 gccatgcgcc atggtctccc tggccccgtc gtcgacgggg tacgctacgt gatcgtcagc
     4381 tcgtccagcc agagcgggct cgtccacgcc ttcttgccgt cgcgatcgat gaccgtaatc
     4441 cgcgcgaagg cgttgccgaa gggcgctatc gggaacactg cgcgcgtcat acccgattcg
     4501 cgcttgtagc ggcaggtgga gccatgaccc cctgccatca ccacctccgc cggcgagcac
     4561 tcgaccacga tctcgtcctc ggtaaaggcc acgttgtgga tctccgggcc ggtgctcgcg
     4621 tagaagcgcc cgagcttgat cgcctccagc agcgcctcgg gctccagcgc ctcggccttg
     4681 acgcagatcc agccgccgaa actgtcgggc cgcacattga agtgcgcgtc atcggcggcg
     4741 gtacaggaga atcgataccc cgccgtggcc atcaggtcgg cgaggaacca gccattgccg
     4801 cggtcgctgt cattctggtg gccctcgttg tggatttcga tcgcgtcaaa gtcctcgatc
     4861 ttgcgggcat cctttggcgt gacgccggtc cactgcggat gcggcagcga gatgaaggca
     4921 ccttccgctc tggcacgggc ggcgatctcg gcaccgtcct cgcccggtcg ctgcgggtcg
     4981 aagtccacgg gcaggccgag cgcgatgatg tgccacagcc caccgttttc gagcccctgc
     5041 ccgtgcagct cggcgccgat cagtgtggtg aagtcggagg tacgcaggtc gcgcgtatcg
     5101 gtgacaggaa agtcgaaccg ctccatgaaa tggtcggtga cggcgacgaa gtcgtagccg
     5161 cggtcccgat agacagcaac gacctccttc ggcgtcattg ttccatcgga attggtactg
     5221 tgggcgtggg tgttgccacg ccagaactgt ccaggctggt cgaaaggcag gttgcgcacg
     5281 tggtttcccc aggagttgga cacaagcgcg cacgcagcgc gcaccgtcac gatcaccatc
     5341 cgccgggaag aatctcctcc ggggcggcgt cctgcggggt gaacccgatt cctctcttgg
     5401 cgtgctcgat atcccagaac cgtcgcgggt tgttggatat accatagcaa agaaaccagc
     5461 gtttgcgcgt tgcgagcgcg cactcgatca ggtgaacgcc gtcgcgatcg ctcagccacg
     5521 tcgctcgata gcgtcggcga ttggcgatcg cctctggatt atcatccttc gcgggatccc
     5581 agagctgctg cggcgtcatg ggcgagtcgg cgcccgtcca ggaaccgatc cgcaggcaga
     5641 cggcactgac accatgatgg tcgacgtaga agcgagccat tgcctccccg taggccttgg
     5701 agacgccgta gagcgaatcc ggctggatct cgacggtgtg atcgatcatg cggccgtcgt
     5761 cgagatcgta gagctccggg cgattgctgg tctcgaaggt cccgaccgca tgattggatg
     5821 acgcgaagac gaccttgttg actcccgcac ggcgcgccgc ctcgaagacg ttgtaggtgc
     5881 caatcaggtt gctgtcacgc acggcatccc actcggaaga aactgccgag gtcgccgcga
     5941 ggtggaccac cgcatcgatc ccatccatcg acgcgacgag cgcgtcgagg tcggtaatgt
     6001 cgccgatgat gtcggtgtag ggcccctcgg accgggcgag cccgatcggc acgaagcgcc
     6061 cgctccgccg ccaagcatcg gtcagcgtgc ggccgatgag gccactggag ccggtaatca
     6121 gcacccggcg cggttgctct ccgctcgcat tcatgcgacg ttgttctcca ctgtctcgtg
     6181 cggcacgttg cgcaggcacc gaccagtgcc agcatccatg caaactgtcg cacaccccgg
     6241 ccgccgcgtg gatggcccac ccgttcccgc cctggagtgc aaacgctgtg cccctgatcg
     6301 ccctcgcccg tcgcctgctg cccggcttgg cgctcgccgc cgggatcacc ctgctggcga
     6361 tcgggatcgc cgggctcgaa acactggtct ttggctaccc actcctggag ccgctggtcc
     6421 tggcgctgat cctcggcgtg gtcgtccgcg cactgtggca accaccggca gtgttcgagc
     6481 cgggcattgc cttcggggcg aagcaggtgc tcgagctcgc gatcgtgctg atcggcgtct
     6541 cgctggcctt gggaacgctg gttgacgccg gggcacggct cgccgtggcg atcctgctgc
     6601 tggtatccat gacgatcgcg ctcggtgtga cgctcggtcg cgccgccggc ctgccggtga
     6661 agctcgcagt gctgatcggg gtcggtaacg cgatctgcgg caactcggcg attgcggcgg
     6721 tcgccccggt gatccgcgcg cgcaagcagg atgtcgccag cgcaatcgca ctgaccgccg
     6781 tgctgggcat cggcgtggtg ctgacgctgc cactgctcgt gccgctgctg gccatctccg
     6841 atccggagta cggtgtggtg gccgggctct ctgtctacgc ggtaccgcag gtcctggcgg
     6901 cgacattccc ggtgagcgcc acggcggggc aggtcgccag cctcgtcaag ctcagccgcg
     6961 tgctgctcct cggcccgacc gtcgcgatcc tggcgatcct cttccgcgag cgggatgccg
     7021 aggccggcag cggcctcacc ttctcgaagc tggtgccgtg gttcgtgatc gggtttggca
     7081 tcgcggtcgc actgcggtcc gccggtctcg tacccgaagc ggtttccgac gcggcacgcg
     7141 agagcagccg agtgctgacg gcagtggcga tggcaggact gggactcagc gtcgacatcc
     7201 gctcggtgcg ggagaccgga tcgcgcgtcg cgctggtggt gctcgggttg acactggtgc
     7261 tggtcacgtc ggcggtcgcc atcaccctcg tcctcgacct gccgtgaggg aagtcagaca
     7321 attggcaacg cggggcagtg aactggggcg gacatggagt ccgcccgtac gggtcggttc
     7381 catgtccgcc cctgtgggtt ctcccgtgga gtgaaggaac cagaaagatg cccacatacg
     7441 tctacacctg cgatgcctgc gaggcagtgt tcgaccggga gcgctcggtc gacgagcgcg
     7501 acgagccgct cccctgcccg gtctgtgaca cgccttcgcg gcgcaagccg gtctcaccga
     7561 agctgctctt caagggcgac ccggccgaga accgtcccta ctggcacaac cacgacggct
     7621 acagccacag ccatgcgccc cgccggggcc gccaccgaac gccggaagag gatcactgac
     7681 caccgaatac acagatgagg acgggcgtca ccgcccgtcc tcatctagcc ttccccctcc
     7741 cctccaaccg ctgtcaacca gcggctgtgg ggccctcccg gagtattcga gctggttccg
     7801 tccaagtgag agcgtattct caacctacac tacagcgaca ggtcgacctt cgcaacatgc
     7861 acctgaaccg ggagtagcac gtcgttatac gtacaatccc tacacccact atagcggata
     7921 tttccgtaaa gtccataccc ctcggcgatt ttccttcgct cacgccgtgt ccagaaccgt
     7981 ctcgaacgat ctatcgagga tctcgatcgc ggcgtcgacg tcgctccggc tcacgatcag
     8041 cggcgggcag atacggagtg tgtttgcgtg cagcccgccc cgcccgacga tcagcccgcg
     8101 ccgcttcgct tcatcctgca cccgcagcac cacgtcggcc gccggctcct tcgtctgccg
     8161 gtcatgcacc agctccaccc cgatcatcag cccgaggccg cgcacgtcgc cgatcaccgg
     8221 ccaccgctcc gcgagctcgc gcagccggtc ctgcatgtac ccgccgatgt cccgtgcgtt
     8281 ctcacgcagg ttgttggtgg cgatgtagtt cagattggca agcgcggccg tcgtggcgat
     8341 cgggttgccg ccgaaggtcg agaggctgag cgatcggatc gagttggcga gctcgtcggt
     8401 cgcgatcacg cctccgatgg gaatgccgtt ggcgagtccc ttggcaaaaa ccacgacgtc
     8461 cggctcgatg ccatacgcct ggaagccgaa gtcggcaacg ccgattcgcc cccaagcggt
     8521 ctgcacctca tccgagatga acgggatgcc gtaccggcgc aggatctcgc ccactcgcac
     8581 gaagtactcg ggcggcgggg taacgaagcc gccgactccc tggatcggtt ccgcgatgaa
     8641 cgctgccggg ttgccgctgg tcgtcgtctg gatcacggcc tcgacgtcct cggcacagaa
     8701 gagatcgcag gaggggtact ccttgcccca cgggcagcgg tagcagtacg ggttgccgat
     8761 gtaatggaca tggagcggcg agagggtcga gctgcgccat ggggtctgcc cactagcgtt
     8821 gatagtcgcg aaggagcgcc cgtggtagga gtggcgcagc gcgagcagct cattgctgtt
     8881 gcggttgagg gtggtgatga gaaacgcggc ctcgttggct tcggtgccgc tgttggtgaa
     8941 gaagaccttg ttgagccgct ccggcgtcat ctcccgcagc cgctcggcca gctcgatctg
     9001 cgaccgaatc aggaagagcg tgctgctgtg cgcgatgcga tcgagctgct ccttgattgg
     9061 ttcgttgatc tcccggatcg cgtgcccgct ggagacagtt gcgatgccac cgaaaaagtc
     9121 gaggtactcg cggccgtcgg cgtcacgaac cagctgcccc tcgccggagc gcagctcgat
     9181 cggctcatcg aagaaaagcc cgaccgaggg gaagaggctc tcccgatgcc gcgcgaccaa
     9241 ctcgtcgtgt cccaacgcgg tggatgcgct ctcgccatcc tgacgcatgc tgctgctccc
     9301 tgtgaggtcg tgatctcgtg cacaggcagg ataccgcagc agcgagccgt tcgcggtagg
     9361 ctatccgatg tgatcgaccg acgcggtccc gccggctcat gccggcatcc cacacagggc
     9421 ctccatggat tcgagagacc tcgtcattat ttggaatgtg cgccgtcatg atcctgatca
     9481 gcgcaacggt gatgcgcgca tcgagacggg accgtgcgat gggtgcggtc ggcattgcaa
     9541 tgggcacgct cgcgctcatc gcctacctcg cccgtagcga tgaggcattg cccgagccac
     9601 aggcggcgcc gtgggtcgtt gaatcaacca caccggacac accagccagc ccgaccattg
     9661 ggccatgagg atcaggactg catgtcggaa accacaggag ctgtcgtcat catcgtcggc
     9721 ctggcaatct ctgccgtcgg ctttctgctg atggttcgga gccagcgaga ggtgctcggc
     9781 ttcgcaatca tcgtggtcgg gttcatgctc gccgggaccg gcatgctaac gatcgaggac
     9841 accgtcgtcg aggagcgcac cccggtcacc ctccccacct ccgcggctac cccggcctca
     9901 taggcgggac gccgatctgt ctccggaagc aaccccgttc ggttaaccca ggcaactacg
     9961 ctgtcattcc gagccgcgcc atgcccgtgg cagggtgcgg ctcgaagaat ctcttgcatg
    10021 acaggcctcg gcgttcgcat ggtgagtcct gttgtgcttg aggtccttcc actccggctg
    10081 ctggccttcg ctcagatagc agcgtgtgat atgtgttgcc acttccggtg gcgggcgggc
    10141 aactccctcc gggaggtccg cccctacggg tccccagcga cgtcgacgac cccgcatcct
    10201 cctgatttat ccggctacga cccactcccc atcgagcatg acgcgagccg ggcgggtggc
    10261 agcgatctcc gctggatcaa cgccctccga gaagacatcc cgctccagca ccgcgagatc
    10321 ggcacggtac cccggcgcga gccgcccgag ctcgccttcg cgtcccatcg cgaacgccgc
    10381 ccaactcgtg tagagagcca gggcctcctc cggcgtcagc acctgatccg caccgagcac
    10441 gcgcccggtg accgtctgcc gcgtgagggc ggtcgccatg cccacccatg ggttgtggtc
    10501 ggccaccgtc gagtcagatg atcctgccaa cggcacccca tggccgaggt aggtgcgaaa
    10561 cggcatgatc cgctgcgccc gttcctcgcc gagcgactgc acaaaactct cgcccagcga
    10621 tcgaatgaac ggcgtgctga tcaccgccgg gatgcggtgc cgagccatgc gctcgagcgt
    10681 gtcgggtgtt ggcaggtagg cgtggtgtac gcggtgccgc aactccggcc tcgggttggc
    10741 ttcctgtgca tcggcaaacg ccttgacgct tacccgctgg gcctcgtcgc cacaggtgtg
    10801 gatgtccatc ggccagccct gagcatgtac ccagcggatg cgctccggca actcgtcctc
    10861 ggggaccgcc cagacgccgt agttgtcagg ctcaccgacg tagggctccg aaacgcgggc
    10921 ggtacgatcg ccgatgccgc cgtccggcag cagcttgacg ccggtgagcc gcgccatggg
    10981 atgacggtcc ggccagcgcc aggccatgcc ggcgatgcga tcctccagct ccggctcctc
    11041 ggtcgccggg acgaaccccc atgtcgccat cagcaaatcg gtccggaccg acatgacacc
    11101 caattccgcg gcgcgcatgt aggcgtcgag ctggtgcggc ggcaaaccgg ggtcgacgac
    11161 cgccgtgagc ccgacgtcgt tgtaggcgcg gcacgctcgc tccaaagcgg cgacgagatc
    11221 gtcctccgtc ggttgcggca tcccgtgcag caccagctcc ttggcccggt cccggaagac
    11281 gccggtcgga tggccgtcct cctcccggtc gaagccgccg gcgtagttga cgtcgacggg
    11341 gggatcgggc gtggtcgcat cgatgccgag ctcgcgcaac gcccgtgtgt tcacgaccag
    11401 tttgttccag acgcgctgca gcacgacggg gtggtccggc gcgacggcgt ccagatcgtg
    11461 acgagtcggc atgcgcccct cggcgagcag cgtctcgtcc cagccgcgcc ccagcaccca
    11521 cgtgcccgga gcgacgcctg ccaccgcctg tcgcacgcga tcctgcagct cgccgatcga
    11581 ccgcacgtca tacagggcat gctcgccgag cagttgcccg gtcgccagc
//
LOCUS       DASYWL010000271         3564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_164059, whole genome shotgun sequence.
ACCESSION   DASYWL010000271 DASYWL010000000
VERSION     DASYWL010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164059"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VGR22_09700"
     CDS             complement(<1..330)
                     /locus_tag="VGR22_09700"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2128879.1"
                     /translation="MSRHTHILSNRVSRRAAVRGGLGLSAGLALTGRIAPGALAQQPT
                     EVTLALDWYPNANHAGLYLAKDRGYFEDAGLGVEIYTPADPAAALQTVGAGRDTFGIT
                     YHSEILFA"
     regulatory      complement(326..437)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            542..1345
                     /locus_tag="VGR22_09705"
     CDS             542..1345
                     /locus_tag="VGR22_09705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02569 family protein"
                     /protein_id="HEV2128880.1"
                     /translation="MTTFPRVSVRSAFGCTEMPVPLAGGQGEAFRCGDIVLKPCHDAE
                     EGVWLARLHLDITPDGFRLPRPVRARSGEWIVNGWQAWTRIEGEHDTNRWSEVIATCR
                     AFHRATAAIARPAFIDSRCSRFARADRIAWDEAPTDCVPQIRSTIEQLQSLLKPVHLP
                     GQLIHGDFTENVLFADGVPPAMIDISPCWRPAAYAQAIVMVDALDWCGADASILELVT
                     DIPEIDQLLARAEIFRIAILDGFHQQGADTLGVLSGHLRTVAMLTDRLG"
     gene            complement(1448..2005)
                     /locus_tag="VGR22_09710"
     CDS             complement(1448..2005)
                     /locus_tag="VGR22_09710"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEV2128881.1"
                     /translation="MAQRAAPPPFLTVDEYLELEEGSSIKHEYVEGALYALAGSSLRH
                     NRIAINILRVLDAAADGTPCQVYISDVRCKVGNVFYYPDVMVACGEPDTDNPVYRSDP
                     CLLVEVASPSTASNDRREKLLIYRQIPTVHAYLIVDQDTRRVERHFRDADGVWQRADH
                     LNDGVIPVPCPEAELTMDGIYRDIS"
     gene            2106..2237
                     /locus_tag="VGR22_09715"
     CDS             2106..2237
                     /locus_tag="VGR22_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128882.1"
                     /translation="MGTTHQPDILTSMLGYGGMVLALADAAGIVGLSGASRLLWHRP"
     gene            complement(3065..3310)
                     /locus_tag="VGR22_09720"
     CDS             complement(3065..3310)
                     /locus_tag="VGR22_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128883.1"
                     /translation="MPGGDHQLVALVEPILLDEGVAAGVAAGVASCALAFLPSVSTSP
                     VPMPLPTTPRRLQRRASTSCIVSPRPSVESTTSRSPS"
     gene            3293..>3564
                     /locus_tag="VGR22_09725"
     CDS             3293..>3564
                     /locus_tag="VGR22_09725"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HEV2128884.1"
                     /translation="MVAAGHTAVPFLRWGDPIMAGAPEWDIENQSAEAQEQQFGYNCD
                     YIAYLPLPQGSDSSDHGLLWVNHEYTNPELMFPGYLSPNPEFDPTEA"
BASE COUNT          625 a         1116 c         1220 g          603 t
ORIGIN      
        1 ggcgaagagg atctcggagt ggtaggtgat gccgaaggtg tcacggccgg caccgacggt
       61 ctgcagcgcg gcggccgggt cggcgggggt gtagatctcg acgccgaggc cggcgtcctc
      121 gaagtagcca cggtccttcg cgagatagag gccggcatgg ttggcgttgg ggtaccagtc
      181 gagcgcgagg gtgacctcgg tcggctgctg cgcgagcgcg ccgggggcga tgcggccggt
      241 cagcgcgagt ccggcgctga gaccgagacc gccccgcacg gcggcgcggc ggctgacgcg
      301 attggacagg atatgggtat ggcgggacat ggttcctccg attcctgcgc gggcattacc
      361 ccgatcaggt tctacggtcg gtcgcgcctt gggcgaccct ctcagcccgg cacctccggg
      421 ctcccgttac ggcagaagtc ttcgatttgc cggcatgcta ccacagagac gaagggggat
      481 ttgctccggt tgaggcagac attggctgcg acagacattc gcaggtcgcc tggaagcgac
      541 aatgaccact tttccacgcg tgagcgttcg atcggcgttt ggctgtacag agatgcctgt
      601 accactggct ggaggtcaag gcgaggcgtt ccggtgtgga gatattgtgc tgaagccctg
      661 ccacgatgcc gaggagggag tctggctcgc acggctgcat ctcgacatca ctccggacgg
      721 ctttcgacta ccgcggccgg tacgcgcgag gtcgggcgaa tggatcgtga acggttggca
      781 agcatggacg cgcatcgagg gtgagcacga cacgaatcgc tggtctgaag tgatcgcgac
      841 ctgccgtgca tttcaccggg cgacagcggc gatcgcgcga ccggcattta tcgacagtcg
      901 atgctcacgg ttcgcgcggg ccgaccggat cgcatgggac gaggcgccga cggattgcgt
      961 tccgcagatc cggtcgacca tcgaacagct gcaatcgctg ctgaagcctg tccatctgcc
     1021 gggccagctg atccatggtg acttcaccga aaacgtcctg ttcgccgacg gagtcccacc
     1081 ggccatgatc gacatcagcc catgctggcg accggctgcg tacgcccagg ccatcgtcat
     1141 ggtggacgcg ctcgactggt gtggtgcgga cgcgtccatc cttgagctgg tcaccgacat
     1201 tcccgagatt gatcagctct tggcgcgggc cgagatcttc cggatcgcca tcctcgacgg
     1261 cttccaccag cagggcgcag acacactggg cgtgctgtcc ggtcacctgc ggacggtcgc
     1321 catgctgacg gaccggctcg gatagtcgat gcagggcgtc cgtggcgcga caaagggagg
     1381 cgtctggacg gatgctcggc tggggggcgc tgatcgggga gtcatgccga caagtgacgt
     1441 cgtcccgtca cgagatgtcc cggtagatgc cgtccatggt cagctcggcc tcggggcagg
     1501 gcacggggat gacgccgtcg ttcagatggt cagcgcgctg ccagacgccg tcggcgtcac
     1561 ggaaatggcg ctcgacacgg cgggtgtcct ggtccacgat gaggtacgcg tgcacggtgg
     1621 ggatttggcg gtagatcagc agcttctcgc gccggtcgtt cgacgcggtg ctcggggagg
     1681 cgacctcgac gagcaggcag ggatcgctcc ggtagaccgg attgtcggtg tcgggttccc
     1741 cgcaggccac catcacgtcc gggtagtaga agacgttgcc gaccttgcac cgtacatcac
     1801 tgatgtagac ctggcatggt gtgccgtccg cggcggcatc cagcacccga aggatgttga
     1861 tggcgatgcg attgtgccgg aggctgctgc cggcgagggc gtacagcgcg ccctcgacgt
     1921 actcgtgctt gatgctggag ccctcttcga gctccaggta ctcatcgacg gtcagaaacg
     1981 gtggcggtgc cgctcgttgc gccatgccgt cagtatatcc caaaccatgc gcacccgatg
     2041 ccgatcgtcc cgctgccgag ccgccagact gaatccagaa ccggacaacg caaggaggtg
     2101 ttcagatggg cacgacgcat caaccagaca tcttgacctc catgctgggc tacggtggga
     2161 tggtgctggc gctcgccgat gccgcgggaa tcgtcgggct cagtggcgcg tcacgcttgt
     2221 tgtggcatcg tccgtagggg ccgacctgcg cagcagtggg cggcccggcc aacggtaggg
     2281 gggcaactgt ggttcgggcg gacacaaggt ccgcccctac accacggcca ccgggcggac
     2341 ctcgggcaca agacccgagg ctacagcggg aggtccgtcc ctacgaggca gattgacctt
     2401 gacgcaccac tcagcgaggg cttgtccggt gtggccagct tgcccgcggc tgcggcgatc
     2461 ggattgatgg catcattcct ggtcgcgacg gtactgctgg tgatcccgga gacgcgcgac
     2521 aacccgctga tctgcccgct ggcgcacgtc aggtcggggc tcctcgaggc gctgcggaac
     2581 agggttacac gccaaagtca gtcgacgaaa cctcacccac tgacgggcag gtccctgcgg
     2641 tcattgtcag ggccgtacgg agcccggccc gccaaccaca cattggcccc attgaggtca
     2701 tgaacaatta acgacaccgc cgggatacgc cgtggtataa agcggatact cgtcaaccca
     2761 cagggcgatg cccgggtgcc actgtcccgg ctggcaagcc actggtcatg acctcgttgt
     2821 ctcccgcgag acggcgagcc gatgcatcct catatggaaa ggagggctcc ccaccactcc
     2881 atcgggtccc tcggcggact ctcgcgttca tgattgttga accacacgtt agcaccgcgc
     2941 tcatgattcc atgctggcca ggcgctctac ttggaccaag cgcacaccac aggcgccagc
     3001 gggacgtgtc tccagcttct ggggacgatc gggaaggaac caccatgaag cgaagcgaag
     3061 agttctacga aggcgatcgt gatgtcgtgc tctcgaccga gggtcgcggc gagacgatgc
     3121 aggaggtgct cgcgcggcgc tggagccgcc gcggcgtggt tggcagtggc atcggcaccg
     3181 gactggtgct gacgctcggc aggaacgcga gcgcacagga cgcaacgcca gccgccaccc
     3241 ctgccgcgac gccctcgtcg agcaggatcg gctcgacgag ggcgacgagc tggtggtcgc
     3301 cgccgggcac acggcggtgc cgttcctgcg ctggggcgat cccatcatgg cgggagcgcc
     3361 ggagtgggac atcgagaacc agtcggccga ggcccaggag cagcagttcg gctacaactg
     3421 cgactacatc gcctacctgc cgctgcccca aggttccgac agctccgacc atggcctgct
     3481 ctgggtcaac cacgaataca ccaacccgga gctgatgttc ccgggatacc tgtcgcccaa
     3541 ccccgagttc gaccccaccg aggc
//
LOCUS       DASYWL010000272        14632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_576904, whole genome shotgun sequence.
ACCESSION   DASYWL010000272 DASYWL010000000
VERSION     DASYWL010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 14632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14632
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576904"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..491
                     /locus_tag="VGR22_09730"
     CDS             66..491
                     /locus_tag="VGR22_09730"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEV2128885.1"
                     /translation="MISVELWPQPAVRFWVLAQRFRKFLTVGAFGLLVNQAVLAMLHD
                     GLEIAVVMASPVAIFLSMMVTFALNEHWTWHDRGTGRVISRAMTYVPINLGGLLINWG
                     VLYYLHEDHGMHYLAANLIGAGIAAIWNFLLNHSITWRR"
     gene            603..2084
                     /locus_tag="VGR22_09735"
     CDS             603..2084
                     /locus_tag="VGR22_09735"
                     /inference="COORDINATES: protein motif:HMM:NF005548.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2128886.1"
                     /translation="MQNGHNRETARPSTIIMGAGPAGLCSAYVLSKAGYPATVVEAAP
                     FVGGLARTIKRQTDHGEFKFDIGGHRWFTKNDELNDLFREVVADELLWVKRISRIYFD
                     GKFVDYPLKLTNALKAVGPVTAARAVFDYGLIQAKRKMNPQDVVSMEDAYIAQYGQTL
                     YELFFQAYSEKVWGMKCSELSGDWVSQRTKGMSLVTAIKDAVVPSRGKVVSLIDEFMY
                     PRYGFGRFSERMADAIEAAGNEIKLKSPVEKILLEGNRVKGVQVRTEDGSTVIEGDNF
                     ISSIPMTVLVKITDPLAPQEVIEAANSLVFRDIITVNMMFKKRQVTSDTWLYVHDRNI
                     LFGRFHEPKNWSPDMVPGEEYTSLVVEYFCTRGDYIWGMTDEQLVNQTVKHLVEDLGF
                     VEPSEVIGGFVVRATKAYPTYDIGYQEPLRLMKDYIASIENLQYIGRGGSFRYNNTDH
                     SIETGLLAAKNILGEHHDLEMVNADAEYHEIKRRREAAAAASS"
     gene            2169..2573
                     /locus_tag="VGR22_09740"
     CDS             2169..2573
                     /locus_tag="VGR22_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128887.1"
                     /translation="MSENAAEKLPAEQPDEQLAARADPGRSGASMARVGVWTFAALAL
                     LVYLAVGRVDAVWYRYNIATQRMSRQVDERNIVNVWMSWLDNLPESPDDWVLNALFVG
                     SLVVVVVVMMLGAWLLLVSSGDLNPRASRSRR"
     gene            2570..4594
                     /locus_tag="VGR22_09745"
     CDS             2570..4594
                     /locus_tag="VGR22_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128888.1"
                     /translation="MRSEQRIETRPFLLRRQAIPALWVVMFATLAGWVIWRLELFPLA
                     QRVLIDGTSISEPRAFFTVDHPFHTARASLIARSWEEFEALRWVGSHQGGYPAEFFPF
                     GVPAVAALIALLTGGVLEIASAWAVTVAALFLLPAVGYLLFGRHDRLSPAFAFVASAG
                     HVTIASDWTHGGYSELVEWGLATNAAGATYALLALPLLLSAARPDGVRWIPVAAVVIT
                     LCMVSNPRSLIAVAVGAIAVIAQRSLVGDRRQAILRVGAVAVLAIGLSAPVILPLLRY
                     SDLYFFLSYQEYDSAGEFLAATVDSVTWPVLVAAVAGSALAFRRSEHRSTQAAAIALA
                     FYVALTALAAALPELRQLIPQLELPRLMPFQRLLTLWLAAYGIMRLVSCIPVGKRTWR
                     DAGAAMAVAAVLAVIFTTSLGPFVPDAHGLREVPRIEGEEAVELIQFREAIEEADAVA
                     LPDAAILVLGSRLSWHEQLWAPMVTEGRRFLYNDWLWYWHRLHEGPYDYRGGHFYPNP
                     GDALEPEYLATHGIGAVVVMDVGDASLRASRSPDLEPVSTIGAWDVYQVTSPTGIATL
                     DGAAPDSVAVSDDFERIIVAFEDADPGTLFVHQNWFPRWGATANGESVPVDRGESGYI
                     AVPIMEGGAVEIELTYNVTTLDAIARGVAVASGVVALFLILRPISRWARR"
     gene            complement(4563..5498)
                     /locus_tag="VGR22_09750"
     CDS             complement(4563..5498)
                     /locus_tag="VGR22_09750"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEV2128889.1"
                     /translation="MLRIAERLNAPVVTSIQGKGSVRDSHPNTLGSPWRRGNAVDELV
                     RQSDALLVIGSRLGVQATQRFTFPVPEKLVRIDIDPEEMTRNATPAIGIEADARHAAG
                     LLADALSAQDSASVWNSEQFEVARTEARKTALGAARMPYLDAIRSAIPAEGIIAWDMT
                     MMSYVATYSFPVERPRTFLFPTGHGTLGFSVPAAIGAKIGVGDRPVAAVVGDGGFQFT
                     MQEVATAIQFELGIPIIIFNDSTYSAVKEEQQSSRGGRFAAVDLVNPDYVKLADAYSI
                     PGVRANSPDQLRAVITDALGRNVPTIIDVPIEKWV"
     gene            complement(5462..6202)
                     /locus_tag="VGR22_09755"
     CDS             complement(5462..6202)
                     /locus_tag="VGR22_09755"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEV2128890.1"
                     /translation="MTDVLPAERAAQAASDMTAAQAAIHQLEACGATTVFGIPGVHTL
                     ALYDALLDSSMTHILARHEQGAGFMADGFARATGQPAVALVITGPGVTNVATPIGEAY
                     TDSSPVFVLSSNVEKAHLDAMRGNLHDLKDQRAVMRAVTKWHDRATEAGDVPRLVAEG
                     WQAMTSGRRLPVHLEVPLDVLDEPIGDAAIEPCEDQTTSADIANEVEQAAEMLSGAKR
                     PVIYAGRGRPPQGRARPSSASQSDSMRR"
     gene            6353..7564
                     /locus_tag="VGR22_09760"
     CDS             6353..7564
                     /locus_tag="VGR22_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128891.1"
                     /translation="MTTGATRTDLDYHGSHLPRGSFARSLFDNQLIRMSDRGGASQLI
                     GDAWSEVVARELEDWVGVAIPSERPALASSRVAAVHRLDTMPGVAARASKIGLKNPDF
                     VVFALREGHPLIVAVDAKFLVETARAEQVSAETMRLLFEHDPHLIELLPDRDPEARYA
                     DGLFLAPDYSLTHAMFQRKVGHRRMTVSPHDVVLTDVGAREMFGRAASEAVMDRLIAI
                     DDAAFPVWESLLAAQYYFRLERAVAGLDVESRKPLLGAAEIVVSEDALLTQIGERALG
                     VSSAWELVLEWDRDVEIVRRQRQALHQVIGSPLSSAELRELSDDLLDRLEIEPRPSRN
                     RVRKALGARFTADVLALVDVIEPPVADFSTELQRVAEVAREVGSRYESDIREIVGQII
                     LDLAGEGQVAE"
     gene            complement(7697..8959)
                     /locus_tag="VGR22_09765"
     CDS             complement(7697..8959)
                     /locus_tag="VGR22_09765"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2128892.1"
                     /translation="MKATPETTIRDGQQPRPRIAVPRTFQSLVDYPMFRQLWLAMMAT
                     SISQWTAQVTFGWLALVLTDSARFVGIVSFMSGLPFLVIALPAGVYIDRLDRRKILLT
                     AQSSAALLGIVMAIVVLAGWVQPWHLLVAAFLNGSFLSVLNPTMQSVIPGIVGKDGLT
                     NSVGLMSAGQNMTRIVGPSMAGVTIGFSSTGFAFLLIAVTLLVAVLLISKVRVPDRAA
                     SVTAKGWQAAFSGLQIIWNRGDLRVLFVLACLPPLLIFPYIQFLNIFARDILEIGAQG
                     LGVLTAASGVGAVIGSLMMASRTFRRGDGIRQIRIILLYALGVIGVAFSTTLWITLPL
                     LVMGGMLGGTYMSTNNALLQLRIEDEVRGRVMATYMMTFGLLPLGALPMGLVADALGI
                     QLAVALGSTSCTALILVIALHSKSLRKI"
     gene            9199..9699
                     /locus_tag="VGR22_09770"
     CDS             9199..9699
                     /locus_tag="VGR22_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEV2128893.1"
                     /translation="MTDTQTMQKTRIRMTVNGQERTGEVESRTLLVHFLREQLGLTGT
                     HVGCDTSQCGACTISVNGRSTKSCTMFAVQADGAEVLTIEGLAQDGGYHPVQEGFWEE
                     HGLQCGYCTPGMIMSTVALLKRNPDPTDEEIRHELEGNICRCTGYENIVKSVRYAAER
                     LRESGA"
     gene            9746..10594
                     /locus_tag="VGR22_09775"
     CDS             9746..10594
                     /locus_tag="VGR22_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEV2128894.1"
                     /translation="MFPGSFTYSRPGSVAEAVAALQANPDAKIIAGGHSLIPAMKLRL
                     AMPGELVDLRDIGELRGVEVSDDAVAVRAMTTYNELRDNDEVAGLMPILPEAIQIIGD
                     AQVRERGTLVGSLVHNDPAADLTAVALALGGSVHAVGSDGERDIPLDEFFVDLWTTTL
                     ELDEVVTHASLNRHDGAHMAYRKYAHPASGYAIVGVAVVVEKDGDTVSRARVAVTGAT
                     NTATRLTSIEEALAGKPLTEDNVQAAVENAAEELEINGDTFASAEYRAHLVRVLARRA
                     LLGTQQ"
     gene            complement(10715..11503)
                     /locus_tag="VGR22_09780"
     CDS             complement(10715..11503)
                     /locus_tag="VGR22_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128895.1"
                     /translation="MRQESPPSGRRAAAPRYRPEQPPYRVPRAETEYIYEEDDFDAYE
                     EADAVDDQVIEDERGYAPPPRQRRTSRPAARRRVAHETYADPRYEADYLDDEYDDDEG
                     YEDSFIDEDDWYEEEAAAGAYSPSRQRAPRQRRPMPRPTIPRPVMPARIKQAALVQDQ
                     TALILIGALLLSLAAMATLTFNRADTLAPGFATHISASGVREEFRSETALWQLPLMAG
                     TLLLMNAVLAWALAGYSRFSSRFLLGASIIVHILIWVALIRIAF"
     gene            complement(11737..13182)
                     /locus_tag="VGR22_09785"
     CDS             complement(11737..13182)
                     /locus_tag="VGR22_09785"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV2128896.1"
                     /translation="MNSSFGRLSVWARIRRSLGIGEPTTSAGRRLSRRDREQQNTRML
                     AATLAIVAVLTVISLAAGLAFENVVKPKAVLATVGDEEITRREYWQYHTVQLYQQANE
                     YEEFAQQIDGPQRTQFLSYASSFRAQADDIWGTTEVSEPTLAQMVEDQLYLAAADESD
                     IDLSESAVEAYALQQFAPEGAELTTPFPEPTLIPQRAEWATETAEAQATQVAEELEAL
                     GTPVAGTPVATPVAEADTTADDDVAIHEAATDFAQFRDRALDDANMSLDEYYEMVVRP
                     ELAREAVNAAVVAEVPQSAEQVRARHILVSTEDLARELYERAIGGADFAQLARANSID
                     TATAPTGGDLGWFTFDQMVEPFAEVAFALDPGSISEPVETQYGWHIIMVEDRDEDRAL
                     TELQYQQAQQRAVQDRLEELRASTEIEAEVDVIPTPSPTPETFFPPADAPTPIPATPV
                     PGTPSADVNSVVATPVIEGPVFATPSDGSVD"
     gene            complement(13318..13707)
                     /gene="rpsR"
                     /locus_tag="VGR22_09790"
     CDS             complement(13318..13707)
                     /gene="rpsR"
                     /locus_tag="VGR22_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEV2128897.1"
                     /translation="MTDNQERNETGTEERSDRPQRSRFGGPQGERREGAPRGGGGGRS
                     RVRRVSSGGGRFSPRRKVCQFCVDRIEHVDYKDISRLRRHVGERGKIEPRRKIGTCAR
                     HQRSVTVAIKRARYIGLLPFASSAPRS"
     gene            complement(13749..14195)
                     /locus_tag="VGR22_09795"
     CDS             complement(13749..14195)
                     /locus_tag="VGR22_09795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV2128898.1"
                     /translation="MASLAKITLIGNLGGDPETRYTPQGTLVVNFSLAVNNRRRDASG
                     NQQENTNWYRVSAFGRLAETMVNLSERGYLTKGKQVYIDGNFEAREWTGNDGQTRTSL
                     DVTAREMQLLGQRGDEAGGEHMGGGGGRQAQQPAGNYDSDMDDVPF"
     gene            complement(14201..>14632)
                     /locus_tag="VGR22_09800"
     CDS             complement(14201..>14632)
                     /locus_tag="VGR22_09800"
                     /inference="COORDINATES: protein motif:HMM:NF013419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEV2128899.1"
                     /translation="TDYRDGYYTVYHFDSAPSNLTELERDLKLDTNVMRYLLVHDDPK
                     AGEKPTEGEDEAAEAEGDTEATTAPAEQAAATTETAQVTERAEEQAQPTEAAPAEAES
                     TPEAAVATGALAGTNESEQTGGAEAAAVAAESDTEETTDKE"
BASE COUNT         2421 a         4579 c         4930 g         2702 t
ORIGIN      
        1 cctgatgtca ggagcttctc atgggcgtgc tatactgact ggttgcgaga gatctgggag
       61 cagggatgat ctcagttgaa ttatggccac aacccgctgt ccggttctgg gtacttgccc
      121 agcgattcag gaagtttctg acggtcggcg cgtttgggct cttggtcaac caggccgttc
      181 tcgccatgct ccacgatggg ctcgagattg cggtcgtgat ggcgtcgccg gtcgctatct
      241 tcctctccat gatggttacc ttcgcgctca atgagcactg gacatggcac gaccggggaa
      301 ccggtcgggt gatctcgcgc gcaatgacct acgtcccgat caatctcggc ggcctgctca
      361 tcaactgggg cgtgctgtac tatctgcatg aagatcatgg catgcactat ctcgctgcca
      421 acctgattgg cgcgggtatt gccgcgattt ggaatttcct gctgaaccac tcaatcactt
      481 ggcgacgcta agaccagtgc gccgtgcaag aatcgccacc gggcaagcag ctcggccctg
      541 ataggtaggt gacgtggcgt ggcatgatct cggcgatgga atgaagggaa ggaattcgct
      601 cgatgcaaaa cggtcacaac cgcgaaacgg cgcgcccctc cacaatcatc atgggagcgg
      661 gaccggcggg tctgtgctcg gcctacgtac tcagcaaggc cgggtatccc gccactgtcg
      721 tggaggcggc tcccttcgtc ggtggcctcg cccggacgat caagcgtcag acggatcatg
      781 gagagttcaa gttcgacatc gggggtcacc ggtggttcac caagaatgac gagctcaacg
      841 acctctttcg ggaggtggtc gccgatgagc tgctgtgggt gaaacgcatc agccgcatct
      901 acttcgacgg caagttcgtc gactaccccc tcaagctcac gaacgccctt aaggcggtcg
      961 ggccggttac ggcggctcgc gcggtcttcg actacggact gatccaggcc aagcgcaaga
     1021 tgaatccgca ggacgtggtg tccatggagg acgcctacat cgcgcagtac ggccagacgc
     1081 tctacgagct cttcttccag gcctacagcg agaaagtctg gggcatgaag tgcagtgagc
     1141 tctcggggga ctgggtcagc cagcgaacca agggcatgtc gctcgttacg gcaatcaagg
     1201 atgcagtggt cccgtccagg ggcaaggtcg tcagcctgat cgacgagttc atgtaccccc
     1261 ggtacggatt cggtcggttc tcggagcgga tggcagacgc catcgaggcg gcgggcaacg
     1321 agatcaagct caagagcccg gtcgagaaga tcctgctgga gggcaaccgg gtcaaggggg
     1381 tgcaggtccg taccgaggac ggcagcaccg tgatcgaggg ggacaacttc atctcctcga
     1441 ttcccatgac cgtgctcgtc aagatcaccg atccactcgc gccgcaggag gtgatcgaag
     1501 cggccaactc gctcgtcttc cgcgacatca tcaccgtcaa catgatgttc aagaagcgcc
     1561 aggtgacgag cgatacgtgg ctctatgtcc acgaccgcaa catcctgttc ggtcgcttcc
     1621 atgagcccaa gaactggagt cccgacatgg tgccgggcga ggagtacacg tcgctggtcg
     1681 tcgagtactt ctgcacgcgg ggcgactata tctggggcat gaccgacgag cagctcgtca
     1741 accagaccgt caagcatctg gtcgaggatc tcggcttcgt cgagcctagc gaggtgatcg
     1801 gcggtttcgt ggtccgcgcg accaaggcgt acccgaccta cgacattggc tatcaggagc
     1861 cgctgcggct gatgaaggac tacatcgcca gcatcgagaa cctgcagtac atcgggcgcg
     1921 gcgggtcgtt ccgctacaac aatacggacc actcgatcga gaccggcctg ctggccgcca
     1981 agaatatcct tggcgagcac cacgatctcg aaatggtcaa tgcggacgcc gagtaccacg
     2041 agatcaagcg ccggcgggag gccgcagcgg cagcgtcgtc gtaagaccac gccgtgcgtc
     2101 atggaggggc cgccgccgat caccggcggg cggccctctt tgcctgcgca gctacgaggt
     2161 gcaaggccat gagcgagaac gctgcggaga agctaccggc ggagcagcct gacgagcagc
     2221 tggcggccag agccgatccg ggtcgatccg gcgccagcat ggctcgcgtt ggcgtgtgga
     2281 ccttcgcggc gctcgcgcta ctggtctacc tcgccgtcgg tcgggtcgac gctgtctggt
     2341 atcggtacaa catagccacg cagcgcatga gccgacaggt agacgaaaga aatatcgtca
     2401 atgtctggat gagctggctc gacaacctgc cggaatcgcc tgacgactgg gtgctcaatg
     2461 cgctcttcgt cgggtcactc gtcgtcgtgg tcgttgtgat gatgctgggc gcctggctgc
     2521 tgctcgtctc ctccggcgat ctcaatccac gtgcgtcccg ctcgcgacgg tgaggagcga
     2581 gcagcgcatc gagactaggc cattcctcct ccggcggcag gcgataccgg ccctgtgggt
     2641 ggtgatgttt gccacgctgg ccggatgggt gatctggcgc ctggagctct ttccgctcgc
     2701 ccagcgcgtg ctgattgatg gaacgagcat ctccgagccg cgcgcctttt tcacggttga
     2761 tcatccgttc cacaccgccc gggcctcctt gatcgcacgc tcatgggagg agttcgaagc
     2821 actccggtgg gttggcagcc accaaggcgg ctatccggcg gagttctttc cctttggcgt
     2881 gccagcggtg gcggcgctga ttgcactgct gactggcggg gtgctggaga tcgcgtcggc
     2941 gtgggccgtg accgtggctg ccctcttcct gctgccggct gtcggttacc tgctgttcgg
     3001 tcggcacgat cggctctcgc cggcgttcgc gttcgtggca agtgctgggc acgtcacgat
     3061 cgcgagcgac tggactcacg gtgggtacag cgaactggtg gagtggggct tggcgaccaa
     3121 cgctgccggg gcgacctacg cgctcctggc gctgccgctg ctgctcagcg ccgcccgccc
     3181 ggatggcgtg cgatggatac cggtcgccgc cgtagttatt accttgtgta tggtcagcaa
     3241 tccgcgctcg ctgatcgccg tggcggtcgg ggcgatcgct gtcatcgcgc agcgctcgct
     3301 ggtgggcgac cgcagacaag caattctgcg cgttggcgct gtagccgttc tcgccatcgg
     3361 cctaagcgcg ccagtcatcc tgccgttgct ccgctacagc gatctctact tctttctcag
     3421 ctatcaggag tacgactcgg ctggcgagtt tctggccgcc acggtcgaca gcgtcacctg
     3481 gccggtactg gtcgctgcgg ttgccggctc ggccctggcc ttccggcgat cggagcaccg
     3541 atcgacacag gcggcggcca tcgccctggc cttctacgtg gcactcaccg cgctcgccgc
     3601 ggccctgccg gagctccgcc agttgatccc gcagctcgag ctgccgcgcc tgatgccgtt
     3661 ccagcggctt ctgacacttt ggcttgccgc ctatgggatc atgcgtttgg tgagctgcat
     3721 tcccgtcggc aagcggacat ggagagacgc cggcgcagcc atggcagtcg ctgccgtgct
     3781 ggccgtcatt ttcaccacga gcctcggacc cttcgtcccg gatgcgcatg gcctgcggga
     3841 ggtgccgcgg atcgagggcg aggaggcagt cgagctgatc cagttccggg aggcgatcga
     3901 ggaggccgac gcggttgccc tgccggatgc ggcgatcctc gtgctcggct cgcggctcag
     3961 ctggcacgag cagctgtggg cgccgatggt gaccgagggc cgccggttcc tctacaacga
     4021 ctggctctgg tactggcacc gactgcatga gggcccctac gactatcgcg gcgggcactt
     4081 ttatcccaat ccgggtgatg cgcttgaacc ggaatatctg gctacccacg gcatcggcgc
     4141 cgtggtggtg atggatgtcg gcgacgcgag tctccgcgcc tcccgaagcc cggaccttga
     4201 gcccgtctcc acgatcggcg cgtgggatgt ctaccaggtt acgtcgccaa caggtatcgc
     4261 gacgcttgac ggcgcggcgc cggactccgt ggcggtctcc gatgatttcg agcgcatcat
     4321 tgtcgcattc gaggacgccg acccgggcac attgtttgtg caccagaact ggttcccgcg
     4381 ctggggggcg accgccaacg gcgaatcggt gccagtggac cgcggtgaga gtggctacat
     4441 cgccgttccc atcatggagg gcggtgccgt ggagatcgag ctcacttaca acgtcaccac
     4501 actcgacgcc atcgcgcggg gggtggcggt ggcgtcggga gtcgttgcgt tgttcctgat
     4561 tctcagaccc atttctcgat gggcacgtcg atgatcgtcg gcacgttgcg gccaagggca
     4621 tcggtgatga cagcgcgaag ctggtccgga ctgttcgcgc gaacgccggg aatgctgtag
     4681 gcatcggcaa gcttcacgta atccgggttg accagatcga ccgctgcaaa gcggccaccg
     4741 cggctcgact gctgctcttc cttgacggca gagtaggtgg agtcgttgaa gatgatgatt
     4801 gggatgccca gctcgaactg gatggccgtc gcgacttcct gcattgtgaa ctggaagccg
     4861 ccatcgccga ccaccgccgc caccggcctg tcgccaacgc caatcttcgc gccgattgcg
     4921 gccggcacgg agaaaccgag ggtgccgtgg ccggttggga agaggaaggt gcgcggccgt
     4981 tccaccggga aggagtaggt ggcgacgtag ctcatcatcg tcatgtccca ggcgatgatg
     5041 ccctcggctg gaatcgctga gcggatggcg tcgaggtagg gcatccgagc agcgccgagc
     5101 gcggtctttc gagcctctgt ccgagccact tcaaactgct cgctgttcca cacggacgct
     5161 gaatcctgcg cgctcagcgc atcggccagc agtccggcag cgtgccgcgc gtccgcttcg
     5221 atgccgatag caggagtcgc gtttcgcgtc atctcctcgg gatcgatgtc gatgcgcacg
     5281 agcttctccg ggacagggaa ggtgaagcgc tgggtggcct ggacgccgag gcgcgagccg
     5341 atcacgagca gggcatcact ctgccggaca agctcgtcga cggcattgcc ccgcctccag
     5401 ggcgagccaa gggtattggg gtgggaatcg cgaaccgagc ccttgccctg aattgacgtc
     5461 actaccggcg cattgagtcg ctctgcgatg cggaggatgg tctcgcccgc ccctgaggag
     5521 gtcgccccct accggcgtag atcaccggac gcttggcgcc cgagagcatc tcggccgcct
     5581 gctcgacctc gttggcgatg tccgccgatg tcgtctgatc ctcgcacggt tcgatggcgg
     5641 catcgccgat cggctcgtcg agcacatcga gcggtacctc cagatggacc ggaaggcggc
     5701 gtccgctcgt catcgcctgc caaccttcgg cgaccaagcg tggcacatca cctgcttcgg
     5761 tggcacggtc gtgccacttg gtgacggccc gcatgacggc gcgctggtcc ttgaggtcgt
     5821 ggaggttgcc ccgcattgcg tccagatggg ccttctcgac attgctggag aggacgaaca
     5881 cgggcgagga gtcggtatag gcctcgccga tcggtgtggc gacgttcgtg acgcccggcc
     5941 cggtgattac cagcgcgacc gccggttggc cggtggcccg agcgaagcca tccgccatga
     6001 agccagcgcc ctgctcgtgg cgtgcgagga tatgggtcat ggaagagtcg agcagcgcgt
     6061 cgtagagcgc gagcgtatgg acacccggga tgccgaacac cgtcgtggcg ccgcacgcct
     6121 cgagctggtg gatggctgcc tgggcggcgg tcatgtcgct cgcggcttgg gcggctcgct
     6181 cggcagggag aacgtcggtc atgcaggtgc ctccatgggg tgatcctgtc gcctgctcat
     6241 gcatcatggg gtagtcgcgg tgatgcgacg gctctcatgg tggcatactc cccgcagcga
     6301 ggcaaaccac ggctggcctg gtgaacgtgg tcggcgatcg aggatgacct gcatgacgac
     6361 cggagccacc cgaaccgatc tcgactatca cggcagccat ctgccgcgcg gtagcttcgc
     6421 gcggtcgctt ttcgacaacc agctcatccg catgtcggat cgcggcggtg ccagccagct
     6481 cattggcgat gcgtggtcgg aagtcgtcgc gcgcgaactc gaggactggg tgggtgtggc
     6541 catcccgtcg gagcgcccag ctctcgccag cagtcgggtg gcagcggtgc atcggctcga
     6601 taccatgccc ggcgttgccg ctcgcgcctc caagatcggg ctgaagaacc cggacttcgt
     6661 cgttttcgcc ctgcgtgagg ggcacccgct gatcgtcgca gtcgacgcca agttcttggt
     6721 ggagacggcg cgcgccgagc aggtgagcgc ggagacgatg cggctgctct tcgagcacga
     6781 tccacacctg atcgagttgc tgccggaccg ggacccagag gcccgctatg ccgacgggtt
     6841 gttcctcgcg ccggactaca gcctgaccca tgcgatgttc cagcgcaagg tcgggcaccg
     6901 gcgcatgact gtctcgcccc acgacgttgt gctcacagac gtgggggcgc gcgagatgtt
     6961 cggtcgggcc gccagcgagg ctgttatgga tcgactgatt gcaatcgacg acgcggcctt
     7021 tcccgtctgg gaaagcctgc ttgccgctca gtattacttt cggcttgaac gggcggtggc
     7081 aggactcgat gtcgagtccc gcaagccgtt gctcggtgca gccgagatcg tcgtgtccga
     7141 ggacgccctg ctgacacaaa tcggcgagcg tgcgctcggc gtcagcagcg cttgggagct
     7201 ggttctggag tgggatcggg atgtcgagat tgttcggcgg cagcgccagg cgctgcacca
     7261 ggtgatcggc tcgccgctct ccagcgcgga gctgcgtgaa ctctctgatg accttcttga
     7321 tcggcttgag atcgagccgc gtccgtcccg caatcgtgta cgcaaggcac tcggcgcgcg
     7381 cttcaccgca gatgtcctgg cgctggtgga cgtgatcgag ccgccggtcg ccgacttcag
     7441 cactgagctc cagcgggtcg cggaggtagc gcgcgaggtc ggcagtcgct acgagtcgga
     7501 cattcgcgag atcgtcgggc agatcatcct tgaccttgcc ggtgagggcc aggtggcgga
     7561 gtagactcgg cgcctgaaac atagttgtag cggcagacct tgtgtctgcc cgcctgtggc
     7621 accaacatca tgccggtgat acgggctgtg gctggcaagc cgccagcgcc ccaaggttcc
     7681 aattcattcg cacacttcaa atcttgcgaa gtgacttcga gtgcagcgcg atgacgagaa
     7741 tgagggcggt acagctggtg gagccgagcg ccaccgcgag ctggatgccg agcgcgtcgg
     7801 cgaccaggcc catcggcagc gcgcccagcg gcagaaggcc gaacgtcatc atgtacgtcg
     7861 ccatcacgcg tccgcgcacc tcgtcctcga tcctcagctg gagcagcgcg ttattcgtgc
     7921 tcatgtaggt gccaccgagc atgccaccca tcacgagcag tggcagcgtg atccacagcg
     7981 tggtcgagaa cgccacgccg ataacaccga gcgcgtagag caggatgata cgaatctgcc
     8041 ggatgccgtc accgcgccgg aaggtccggg acgccatcat cagcgatccg atcactgccc
     8101 cgactccgga tgccgcggtg agcacgccga gtccttgagc gccgatctcg aggatgtccc
     8161 gcgcgaagat gttcaggaac tggatgtagg ggaagatcag caatggtggc aggcacgcaa
     8221 gcacgaagag cacgcgcagg tcaccccggt tccagatgat ctggaggccc gagaatgccg
     8281 cctgccaacc tttggctgtc acactcgccg cgcggtctgg cacacggacc ttgctgatca
     8341 gcaggactgc aacgagcagc gtcacggcaa tcaggaggaa ggcgaacccc gtcgagctga
     8401 agccgatggt tacccctgcc atgctgggtc cgacgatccg cgtcatgttc tgcccggcgc
     8461 tcatcagtcc cacggagttg gtcaggccat ccttgccgac gatccccggg atgaccgact
     8521 gcatggtcgg gttgaggact gacaggaacg agccgttcag gaatgcggcc accagcaggt
     8581 gccacggttg cacccagccg gcgagcacga cgatcgccat cacgatgccc agcagcgcgg
     8641 cgctcgactg cgcggtgagc aggatcttcc gccggtcgag ccggtcgatg taaacgccgg
     8701 cggggagggc gatgacgagg aaggggaggc ccgacatgaa gctcacgatg ccgacgaacc
     8761 gggcggagtc cgtgagcacg agcgccagcc acccgaaggt gacctgcgcc gtccattgcg
     8821 agatggacgt tgccatcatc gcgagccaga gctgccgaaa catcgggtag tcgaccagcg
     8881 actggaacgt gcgcggaacg gcgatccggg gtctcggctg ctgaccatcg cggatggtgg
     8941 tctcgggcgt tgctttcacg aacggagtat agcggtaggg gagaacatgg gaaacggcac
     9001 gacgagatcc ggtgttgtgc aagcgcagca ggtttgcgag tattgtcgca gtcgatgacg
     9061 gggtgaacga gacgtcaatc ctcgccgaac gtcggggtgg cgataccaca gcatagcgat
     9121 gcgggtttgg tacgagacga cggctgtccg gcatcatcga tggccgacgt gcgttggccg
     9181 ctcgagaggg gtacgagtat gactgacacg caaacgatgc agaagacacg aatccggatg
     9241 accgtgaacg ggcaggagcg gaccggcgag gtcgagtcgc gaacgctgct cgtgcatttc
     9301 ctgcgcgagc agcttgggct gaccggcacg catgtcggct gcgataccag ccaatgcggc
     9361 gcctgcacga tcagcgtgaa cggcaggagc accaagagct gcaccatgtt cgccgtgcag
     9421 gccgatggcg ccgaggtcct gaccatcgag ggattggccc aggatggcgg ataccatccc
     9481 gtccaggagg gcttttggga agagcacggt ctccagtgcg gctactgcac gcccggcatg
     9541 atcatgtcca ccgtggcgtt gctcaagcgc aacccggacc cgactgacga ggagatccgc
     9601 cacgagctcg agggcaacat ctgcaggtgc accgggtacg agaacatcgt caagtccgtc
     9661 cgctacgctg ccgagaggct gcgtgagagc ggcgcctgac agcggatttc cgactgtacg
     9721 acgctgaagg caagaggtgt ctcccatgtt tccaggatcc ttcacctaca gccggccggg
     9781 atcggtcgct gaagccgttg ccgcgctgca ggccaacccc gatgccaaga tcattgccgg
     9841 cgggcactcg ctcatacccg ccatgaagtt gcggctcgcg atgccgggcg agctcgtcga
     9901 tctgcgcgac atcggcgagc tgcgcggcgt cgaggtcagc gatgatgcgg tcgccgtccg
     9961 cgccatgacg acgtacaacg agctgcgcga caacgatgag gttgccggcc tgatgccgat
    10021 tctccccgag gcgatccaga tcatcggtga cgcgcaggtg cgcgagcgcg gcacattggt
    10081 gggctcgctg gtccacaatg acccagctgc cgatctcacg gcagtcgcgt tggcgcttgg
    10141 cggctcggta cacgccgtcg gttcagacgg tgagcgggac atcccattgg acgagttctt
    10201 cgtcgatctc tggacaacga cgctggagct ggacgaggtg gtgacccatg cctcgctgaa
    10261 ccgtcatgac ggcgcgcata tggcctaccg gaagtacgcg catcccgcta gcggctatgc
    10321 catcgtcggt gtcgccgtcg tggtcgagaa ggacggcgac accgtctccc gtgcccgtgt
    10381 ggccgtcacc ggcgcgacca acaccgccac ccggctgacg tccattgagg aggcgcttgc
    10441 cggcaagccg ctgaccgagg acaacgtgca agccgcggta gagaacgccg ccgaggagct
    10501 ggagatcaac ggggacacct tcgcgtcggc ggaatatcga gcccacttgg ttcgtgtgct
    10561 tgcccgccgc gcgctgctcg gcacgcagca gtagtttcgg ggcaagggtc gaagatgcag
    10621 gaaaccggga gggtaatcgc cctcccggtt cgccattgcg agccgatgtt ggtcgatacc
    10681 gactcagggt gatggattcg gctgggttcg atggctaaaa cgcgatgcgg atgagggcga
    10741 cccagatcaa gatgtgcacg atgatcgacg cgccgagcag gaagcgcgat gagaagcgcg
    10801 agtatcctgc caaggcccag gccagtaccg cgttcatcag cagcagggtg ccggccatca
    10861 agggtagctg ccagagcgcc gtctccgatc ggaactcctc gcggacgccg gatgcgctga
    10921 tgtgcgtggc gaaccccggc gccagggtgt cggcccggtt gaaggtgagg gtggccatgg
    10981 cggccaggct cagcagcaag gcaccgatca ggatcaacgc ggtctggtcc tgcactagtg
    11041 ctgcctgctt gatgcgtgcc ggcatgaccg gtctggggat ggttggccgc ggcatggggc
    11101 ggcgctgtct gggagcgcgc tggcgcgacg gactgtaggc accggcagcg gcttcctcct
    11161 cgtaccagtc atcctcgtcg atgaacgagt cctcgtatcc ctcatcgtcg tcgtactcgt
    11221 catcaaggta gtccgcttca tatcgggggt cggcgtacgt ctcatgcgcc acccgtcgac
    11281 gtgccgctgg ccggctcgtc ctccgctgtc gaggcggtgg agcgtacccc cgctcgtcct
    11341 cgatcacctg gtcatcgacg gcatcggcct cctcgtaggc atcgaagtcg tcctcctcgt
    11401 agatgtactc cgtctctgct ctggggacac gatagggagg ctgctccggg cgatatcggg
    11461 gtgctgccgc ccggcgcccg ctcggtgggc tttcctgtcg catgggagac cgcttcggct
    11521 gcggcgcatc ctgtctcggg acgtatgcgg ttcgccggcg gggatctcgc cggggctgtc
    11581 gcggctcctc gaactcgtct tcagctggac gtcgtcgccg cggagcgccg ggtcggggtc
    11641 gtcgatcgga tgacatgcag cctccttcgg tcctgccgtg tgccggatat gcaacagcat
    11701 atcgcaggcg ggcaacaggg tgtcgcccgc ctgccgctag tcaacgctgc cgtctgacgg
    11761 ggtcgcgaag accgggccct cgatgaccgg ggtcgccacc acgctgttca catccgcact
    11821 gggcgtgccg ggaaccgggg tggccggaat cggcgtcggg gcgtcggccg gcgggaagaa
    11881 ggtctccggc gtcggcgatg gagttgggat gacatccacc tcagcctcga tctccgtcga
    11941 ggcccgaagc tcctcgagcc tgtcctgtac cgcgcgctgc tgcgcctgct ggtactgcag
    12001 ctcggtcaag gcgcggtcct cgtcgcggtc ctccaccatg atgatgtgcc acccgtattg
    12061 cgtctctacg ggctcgctga tgctaccagg atcgagggcg aatgccacct ccgcaaacgg
    12121 ctcgaccatc tgatcgaacg tgaaccatcc aagatcgcct ccggtcggcg cggttgccgt
    12181 gtcgatcgag ttggctcgcg cgagctgagc gaagtccgcg ccgccgatgg ctcgctcgta
    12241 gagttcccgg gcgaggtctt cggtgctgac gaggatgtgg cgggctctga cctgctcagc
    12301 cgactgcggc acttcggcca cgacagcggc gttcacggcc tctcgggcga gctcgggccg
    12361 cacgaccatc tcgtaatact cgtccagcga catgttggcg tcatccagcg cacgatcgcg
    12421 gaactgcgcg aagtcggtcg ccgcctcgtg gatggccacg tcgtcgtcag ccgtcgtgtc
    12481 cgcctcggca accggggtcg cgacgggcgt gccggcaacc ggagtaccca gcgcctccag
    12541 ttcctccgcc acctgggtgg cttgtgcctc ggcggtctcg gttgcccact cggcgcgctg
    12601 cgggatcaac gtcggctcgg ggaacggggt ggtcagttcg gcaccctccg gcgcgaactg
    12661 ctgcagggcg taggcttcga cggccgactc gctgaggtcg atgtccgatt cgtcggcggc
    12721 agcaaggtaa agttgatctt cgaccatctg tgcaagcgtg ggctcgctca cctcggtcgt
    12781 gccccagatg tcgtctgcct gcgcccggaa ggaagaggca taggagagaa actgggttcg
    12841 ctgcggacca tcgatctgct gtgcgaattc ctcgtactcg ttcgcctgct ggtagagctg
    12901 aacggtgtgg tactgccagt actccctgcg ggtgatctcc tcatcaccga ccgtggccag
    12961 cacggccttg ggcttgacga cattctcgaa ggccaggcca gcggcgaggc tgatcacggt
    13021 cagcaccgcc acgatggcga gtgttgcggc gagcatgcgg gtattctgct gctcccggtc
    13081 tcgccggctg agccgccgtc cggcgctggt ggtgggctct ccaatgccga gcgagcggcg
    13141 gatacgcgcc cagaccgaca atcgtccgaa agatgagttc atgcgacttc cgatcctccg
    13201 aaggcacgcc ggtccgtcgg cgcaaagtaa ggctgcggta cagcaaaggc gcgctgccac
    13261 ccatcatcga gcgggagcgc gcctttagcg gggatgaccg gactgccagc gcgagtccta
    13321 gctccgcggg gcgctgctgg cgaacggcag gaggccgatg tagcgagcgc gcttgatggc
    13381 caccgtgacc gagcgctggt gcctggcaca cgtgccgatc ttgcggcgtg gctcgatctt
    13441 gccgcgctcg cccacgtgcc ggcgcaggcg ggagatgtcc ttgtaatcga cgtgctcgat
    13501 acgatcgaca cagaactggc agaccttgcg gcgcggggaa aagcggccac cgccactgct
    13561 cacgcggcgt acacggctgc gcccgccacc gccaccgcgt ggcgcgccct cacgccgctc
    13621 gccttggggg ccgccgaatc gcgagcgctg tgggcggtcg ctgcgctctt ccgttccggt
    13681 ttcgttgcgt tcctgattgt cagtcattga tgtttgcctc tgttcgggac tgcgcgccgc
    13741 ttcgtgatct agaagggcac gtcgtccata tcgctgtcgt agttgcctgc gggctgctgg
    13801 gcctggcggc cgccgccacc gcccatgtgc tcgccgccgg cctcgtcgcc acgctgaccg
    13861 agcagttgca tctcgcgggc cgtcacatcg agcgaggtgc gcgtctggcc gtcgttgccg
    13921 gtccactcgc gcgcctcgaa gttgccgtcg atgtagacct gcttgccctt ggtgaggtag
    13981 ccgcgctcgc tcaggttgac catggtctcc gcgagccgcc cgaaggcgct gacacggtac
    14041 cagttggtgt tttcctgctg gtttccgctg gcatcccggc gtcgattgtt cactgcaagg
    14101 ctgaagttta cgaccaaggt gccttgaggg gtgtaccggg tctcagggtc accaccgagg
    14161 ttgccaataa gggtgatctt cgcgagggat gccatggagg ctactccttg tcggtcgtct
    14221 cttcggtgtc ggattcagcg gctactgcag ccgcctcagc cccgccggtc tgctcgctct
    14281 cgttcgtgcc ggccagagca ccggtggcca ccgcggcctc gggggtcgac tcggcttcgg
    14341 ccggggcggc ctcggttggc tgggcctgct cctcggcgcg ctccgtcacc tgcgcggtct
    14401 cggtggtcgc tgccgcctgc tcggctggag cggtggttgc ctcggtgtcg ccctcggcct
    14461 cggctgcttc gtcctcaccc tcggtcggtt tctcgccggc cttcgggtcg tcatggacca
    14521 gcaggtaacg catcacgttg gtgtcgagct tgagatcgcg ctcgagttct gtgaggttcg
    14581 acggtgcgga gtcgaaatgg tagacggtgt agtacccgtc gcggtagtcg gt
//
LOCUS       DASYWL010000273         4679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_238544, whole genome shotgun sequence.
ACCESSION   DASYWL010000273 DASYWL010000000
VERSION     DASYWL010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238544"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VGR22_09805"
     CDS             complement(<1..599)
                     /locus_tag="VGR22_09805"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV2128900.1"
                     /translation="MTGSGEARAIFLGGMGEVGKNVLLVETDDDLAVIDFGLGFPDET
                     QHGVDVVLPDVGYLRERREKVRGIYITHGHEDHIGALPYLWEAIGAPIYATRLSAGLI
                     QGKLQEARLDKKTEVKVFDPDERPVLDAGSLRIEPFRVTHSIPDAVGFGIQTPAGLIV
                     HTSDFKFDPTPVDGKPTDYGAIEAFGKRGVELLISDCVH"
     gene            complement(622..1251)
                     /locus_tag="VGR22_09810"
     CDS             complement(622..1251)
                     /locus_tag="VGR22_09810"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEV2128901.1"
                     /translation="MAEPRSAEKQLAVIAQEVKDCTRCELFRAATHGVPGEGSPNARV
                     MLIGEGPGWHEDRQGRPFVGNSGKFLDTLIEKADLKREDVFITNIVKHRPPGNRDPLP
                     DEISACSVWLDQQIEAIDPEVIVTLGRHSMNRYFPGERIGKVHGTSKRIGTRVVVPMY
                     HPAAALHNSSLKGTIEEDFGKLPRYLAEVEQQRSKQQEAEQKPEQGSLF"
     gene            complement(1379..2194)
                     /locus_tag="VGR22_09815"
     CDS             complement(1379..2194)
                     /locus_tag="VGR22_09815"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV2128902.1"
                     /translation="MVRRLPIYLLLTLGVLVAVVPFLVTILASLKTSVELVQGVLSLP
                     EEPQWGNYRRAWVDGNFARFFANSLLVAFGVLVPSIVFSCMAGYAFARYRFHAKSVIF
                     IYLLLGLVVPLQALVIPLFYLMREIGLLDSLWALILPQIAMSMSFGTLLMREAFSSVP
                     RPIVEAAVLDGAGSWRVLWEVMFPLARPFVGTLALMFFIWTWNEFLLPLVLNVDPAYQ
                     TLPVGLLYFQQRWTSDIPVIAAGATIIFLPLAIVFLLFQRQMIEGLTQGALKG"
     gene            complement(2187..3125)
                     /locus_tag="VGR22_09820"
     CDS             complement(2187..3125)
                     /locus_tag="VGR22_09820"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV2128903.1"
                     /translation="MDQATQTHAGGETTAFQPAASRRSLISRLEPFLYLLPALLVFTM
                     FMALPVAGTVLISLTNWDGVALNSATFTGVENYVDAFVSEAFWNAFWHNIVLIPFFVL
                     LPALLGLVPAVLVHQLKLRGARFFQAGLFLPYIMPGVLIGVVWRWLLNPVFGPVNDAL
                     KRMGFDPPTWLGDFTWALPTVGLIGAWAAYGFCYVLFIAGIQKIPTELYEAARLDGAG
                     AWEEVKVVTLPGLRREMAVVFSVNLILALRAFDVIKATTDGGPGDETEVLALYMINSA
                     FNANRVGYGTAIAVMLAVLTLVLSVLVLRLFRESDG"
     gene            complement(3259..4581)
                     /locus_tag="VGR22_09825"
     CDS             complement(3259..4581)
                     /locus_tag="VGR22_09825"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV2128904.1"
                     /translation="MQRRTLLKRTGAAAGVAALGPAVLAYSPATVAAQDGATLEFWDT
                     LNADPRKAIVESLAEAFANESDQVAAVEHRGWPLEELQETLPRVVDSNQGPAVAQVNN
                     GESLMGPMIRAGQLVDISGYVQQYGWAEDQPDNLLTRNMYNEDGTQFGIGQLWGVSAE
                     AEIVGIYYNRAILEEGGIEIPETLAGLEAAFEALADAGQTSLVFGNLDGWTAIHLFGN
                     IHGTMTTRDYLDGIIYRWGGYSFEDESIINAAAKLQDWVSRGFLLEGFEGVSADDANA
                     LFEAGSGAMLMQGSWQAGSIEENLGGEAAGFFLTPPLEEGGTVLHVGGVGIPYSITSN
                     AENPDLAAQLIDTLVSEEAFAAFIEAGLLPAGEIPGDVITEGTVSGELYTAWNLVRSK
                     DAVGHYLDWAAPGFYDVLVSQLQQLLAIEVTPEEFASNLQDYYEDSFE"
BASE COUNT          906 a         1551 c         1421 g          801 t
ORIGIN      
        1 atgtggacac aatcggagat cagcagctcg acgccacgct tgccgaaggc ctcgatcgcg
       61 ccgtagtcgg tcggcttgcc gtccaccggc gtcgggtcga acttgaaatc gctcgtatgg
      121 acaatcagac cggcaggcgt ctggatgccg aacccgacgg catcggggat gctgtgggtg
      181 acgcggaacg gctcgatacg caggctacca gcatcgagca ccggccgctc gtcaggatcg
      241 aagaccttca cctcagtttt cttgtcgagc ctggcttcct gcagcttgcc ctggatgaga
      301 ccagcactga gtctggtggc gtagatcggt gcaccgatcg cctcccagag atatggcagc
      361 gcgccgatgt ggtcctcatg accgtgagta atgtagatgc cgcgaacctt ctcgcgccgc
      421 tcgcgcaagt agccgacatc cggcaacacc acgtcgactc cgtgttgcgt ctcatcggga
      481 aacccgagcc cgaagtcgat gaccgcgagg tcatcatcag tctccaccag caggacgttc
      541 ttgccgacct ctcccatccc gccgagaaag attgccctcg cctcgccgct gcccgtcaca
      601 catcatcctt ccgttgctgg gctagaagag cgatccctgc tccggtttct gctcggcttc
      661 ctgctgtttg gaccgctgct gctcgacctc cgcaaggtag cgcggcagct tgccgaagtc
      721 ctcctcaatg gtgcctttga gggagctgtt gtggagcgcg gccgccggat ggtacatcgg
      781 cacgacaacc cgtgtgccga tcctcttcga cgtgccgtgg accttgccga ttcgctcacc
      841 ggggaagtag cggttcatcg agtgccgccc gagcgtgacg atcacctcgg ggtcaatcgc
      901 ctcaatctgc tggtccagcc aaaccgagca ggcgctgatc tcgtcgggca gcgggtcgcg
      961 gttgcctggc gggcggtgct tgacgatgtt ggtgatgaag acgtcctcgc gcttgagatc
     1021 tgccttctcg atcagcgtgt ccaggaactt gccggagttc ccgacgaagg gtcggccctg
     1081 ccggtcctcg tgccaaccag gcccctctcc gataagcatg acccgcgcgt tggggctgcc
     1141 ctcgccggga actccatgcg tggccgcgcg gaagagctcg caccgcgtgc agtccttcac
     1201 ctcttgggcg atcacggcca gttgtttctc ggcactcctc ggctcggcca ccggtccctc
     1261 ctcctgctgc acgctgcacg tcactcgtga cctgatgagt atacgcgagc gcctctcgca
     1321 cgttcccggc cccggcagcg ctcatgaacg accgtcgata cgcagtcgtc ttcggcacct
     1381 agcctttgag cgcgccctgg gtgagaccct cgatcatctg tcgctggaag agcagaaaga
     1441 caatcgcgag cggaaggaaa atgatcgtcg ctccggcggc gatcacgggg atgtcggatg
     1501 tccagcgctg ctggaagtag agcagcccga ccggcagcgt ctggtaggcg gggtccacat
     1561 tgaggaccag cggcagcagg aactcgttcc aggtccagat aaagaacatc agcgccaagg
     1621 tgccgacgaa cggccgtgcc agcgggaaca tgacctccca cagcacgcgc cacgaccccg
     1681 caccgtcgag gacagctgcc tcaacgatcg ggcgcggcac gctggagaac gcctcgcgca
     1741 tcaacagcgt accgaacgac atgctcatcg cgatctgcgg caggatcagc gcccacaggc
     1801 tgtcgagtag accgatctcc cgcatcaggt agaagagcgg gatcaccagt gcctgcagcg
     1861 gcacgaccag gccgagcaga agatagatga agatcactga cttggcatgg aaccggtagc
     1921 gcgcgaaggc gtagccggcc atgcaggaaa aaacaatgga tggcacgagc acgccgaagg
     1981 caaccagcag gctgttggca aagaaccgcg cgaagttgcc atccacccag gcgcgacggt
     2041 agttgcccca ctgcggctcc tccggcagcg acaacactcc ctgcacgagc tcgactgagg
     2101 tcttgagtga cgcgaggatc gtgaccagaa acggcacgac cgcgaccagc acgccaaggg
     2161 tcaggaggag atagatcggc aaccgcctaa ccatcgctct ccctgaacag gcgaagcacc
     2221 agcaccgaca gcaccagcgt cagcaccgca agcatcactg cgatcgccgt gccgtacccc
     2281 acgcggttcg cattgaacgc cgagttgatc atgtagaggg caagaacctc ggtctcatcg
     2341 cccggaccgc cgtccgtggt ggccttgatc acgtcgaacg cccggagagc gaggatgaga
     2401 ttgacggaga agacgacggc catctcccgg cggaggccgg ggagcgtgac aaccttgacc
     2461 tcttcccagg cgccggctcc gtcaagccgc gccgcctcgt acagctcggt cggaattttc
     2521 tgtattcctg cgatgaagag gacgtaacag aagccatagg ctgcccaagc gccgatgagt
     2581 cccactgtcg gcagcgccca ggtaaaatcc ccgagccagg tgggaggatc gaaacccatc
     2641 cgcttcaacg cgtcgttgac cgggccgaac accgggttga gaagccaccg ccagaccaca
     2701 ccgatgagca caccgggcat gatgtacggc aggaagagcc cggcctggaa gaaccgagcg
     2761 ccacgcagct tcagctggtg caccagcacg gcgggcacga gacccagcag cgcgggcagg
     2821 agcacgaaga acgggatcag gacgatgttg tgccagaagg cgttccagaa tgcctcgctg
     2881 acgaacgcat ccacgtaatt ctcgacgccg gtgaacgtcg ccgagttcag cgcaacgcca
     2941 tcccagttcg tcagactaat gagtacggtc cctgcaacgg gaagcgccat gaacatcgtg
     3001 aagaccagca gcgccggcag caagtagaga aacggttcga gccgcgagat cagcgaccgc
     3061 cgcgacgcag ccggctggaa tgcggtggtc tcacccccgg cgtgggtctg cgtcgcctgg
     3121 tccacggagt ccctcctcgc tcgcttgcga agcgatggtc gggcgagtgt cgtgcataca
     3181 agccatgata cgacgaacgg ggaggcacat gtggcctccc cgttcgtcgt aggtttgctc
     3241 gcctggctgg ctacgcgcct actcgaatga gtcctcgtag taatcctgca agttcgacgc
     3301 gaactcctcc ggtgtcacct cgatcgcgag cagctgctgc agctgggaga caaggacgtc
     3361 gtagaagccc ggtgctgccc aatcgaggta gtgaccgacc gcatccttcg accgaaccag
     3421 gttccacgcc gtgtagagct cgccggacac cgtaccttcc gtaatcacgt ccccgggaat
     3481 ctcgcccgcc gggagcaaac cagcctcgat gaacgcggcg aacgcctcct cggacaccag
     3541 cgtgtcgatc agctgggcag cgagatccgg attctcggca ttggacgtga tcgagtaggg
     3601 aatgccgacg ccgccgacgt gcagcaccgt accaccctct tccagcggtg gcgtcaggaa
     3661 gaatccggcc gcctcgcctc cgagattctc ttcgatcgag cctgcctgcc agctgccctg
     3721 catcagcatc gcgccggagc ccgcctcgaa gagggcgttc gcgtcatccg cgctgacacc
     3781 ctcgaagcct tcgagcagga agccgcggct cacccaatcc tgcagcttcg cggccgcgtt
     3841 gatgatcgac tcgtcctcaa aggagtaccc gccccatcgg tagatgatcc catcgaggta
     3901 gtcgcgcgtc gtcatcgtcc cgtgaatgtt gccgaagaga tggatcgccg tccatccgtc
     3961 aaggttgccg aagacgagcg acgtctggcc cgcgtcagcg agggcctcga aggccgcctc
     4021 cagtccagcc agcgtctcgg ggatctcgat cccaccttcc tcgaggatcg cgcggttgta
     4081 gtagatcccg acgatctccg cctcggcgga gacaccccat agttggccga tgccgaactg
     4141 cgtgccgtcc tcgttgtaca tgttccgcgt gagcaggttg tcgggctgat cttcggccca
     4201 accgtactgc tggacgtagc cactgatatc gaccagctgg ccggcgcgaa tcatcgggcc
     4261 catcagcgac tcgccgttgt tgacctgcgc gaccgccggc ccctggttgc tgtcgacgac
     4321 acgcggcagc gtctcctgaa gctcctcgag cggccagccc cggtgctcaa ctgccgccac
     4381 ctgatcgctc tcgttcgcga acgcctccgc cagcgactcg acgatcgcct tgcgcggatc
     4441 agcattcagc gtgtcccaga actcgagggt ggcgccgtcc tgcgcggcaa ccgtcgcagg
     4501 gctgtatgca aggacggccg gccccagagc ggctacaccc gcagccgcac ccgtccgctt
     4561 gagcagtgtt cgacgttgca tggtcatgag ctcattgcct ttcagcttgc tgccgcgaca
     4621 ctatcagtcg ggacttcgag gcagcgccga ttgtgattcc tgtgcgggca tctttacac
//
LOCUS       DASYWL010000274         2397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_558366, whole genome shotgun sequence.
ACCESSION   DASYWL010000274 DASYWL010000000
VERSION     DASYWL010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558366"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..1497)
                     /locus_tag="VGR22_09830"
     CDS             complement(157..1497)
                     /locus_tag="VGR22_09830"
                     /inference="COORDINATES: protein motif:HMM:NF016104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HEV2128905.1"
                     /translation="MVTETADQTTISAPRRRYSRRRPPADRWTFPLLVFAVHWVVAQV
                     PATLTFLYGTIRPSAPGTRSGESWAYGDPLGSMSGLAHWLVEPMRQWDGTWYRLVAVS
                     GYEPMGSFPAKAAFWPLYPWLMDLGSSLTGISPESVGYIISNVAFAGALVLLYHLVTF
                     DFDVPVARRTLWALALFPTAFFFNAVYTESLFLLFTVATLLAARARNWWLAGILGLLA
                     ALTRSAGVMLLAPMGILFLQQYGSNIRRWFPSIFPAALPALGPLIFGWRLTTSGMQFL
                     DWINVQWQWNRFRAMPWETFECATVGCLAEVPNLGAFEARGVDWSWVEMLLASPTWRT
                     ITSYEFRDAFARGDWLELLVTIGAFALLLIGVRRVPLYYFALVLPPLIVPLFSPSSVH
                     PLMSMPRFVLPMFPLFVIIALLVSNRAVGITLGVLSTVLMMALSAQFATWYWVS"
     gene            1694..2254
                     /locus_tag="VGR22_09835"
     CDS             1694..2254
                     /locus_tag="VGR22_09835"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEV2128906.1"
                     /translation="MNAKSLIGMTVFAVDAGKNLGSVDRLLFSTERMRVTGLVIAPAT
                     GLMDEPRPQKLLPTDKVKAVGHDAITVDSESLLEVLADGELPAGAVAFDQIERERVLT
                     ESGEEIGEVSSVEFDELTFQLDYLEIRRGFLSGSSMIAVESVVSVGEDVIVVRDTALE
                     DAAGRDRAEEDRVEHEDDDGMIIERR"
BASE COUNT          484 a          689 c          860 g          364 t
ORIGIN      
        1 gtggaacagt gttcggtcgg cggtacccgt atgcgacccg gctcggccca cggcattgta
       61 cccgcaagat ggcagggtgg ataggggcgg acggcagagg gccgatgcaa ggtcgcctcg
      121 tggcggctgg cttcgtgttc gccctgcact gaggtgtcag gagacccagt accaggtggc
      181 gaattgggcg gagagcgcca tcatgagtac ggtcgaaagc acgcccagag tgatgccaac
      241 cgcgcgattg ctcacgagta gcgcaatgat cacgaagagc gggaacatcg gcagcacgaa
      301 gcggggcatc gacatcaggg ggtgtaccga actcgggctg aagagcggca cgatcagggg
      361 gggcagcacc agcgcgaagt agtagagcgg gacgcggcgg acgccgatga ggagaagtgc
      421 gaatgcgccg atcgtcacga gcagctccag ccagtcgccc cttgcgaagg cgtcacggaa
      481 ctcgtatgag gttatggtgc gccaggttgg cgaggcgagg agcatctcca cccagctcca
      541 gtcgacgccg cgcgcctcga atgcgccgag attggggact tctgcgaggc agccgacagt
      601 ggcgcactcg aacgtctccc atggcatggc gcggaatctg ttccattgcc actggacgtt
      661 gatccagtca aggaactgca tgccggacgt ggtgagacgc caaccgaaga tgagcggacc
      721 gagcgccggc agcgccgccg ggaagatcga cgggaaccag cggcggatgt tgctgccgta
      781 ctgctgcagg aagaggattc ccatcggggc gagcagcatc acgccggccg agcgggtgag
      841 tgccgccagc aggccgagga tacccgccag ccaccagttg cgtgcgcggg cggcaagcag
      901 cgtggccacg gtgaacaaca ggaagaggga ttccgtgtag accgcgttga agaaaaacgc
      961 ggtcgggaag agtgccaggg cccagagggt ccgccgggcg acagggacat cgaaatcaaa
     1021 tgtgaccagg tggtagagca gcaccagtgc cccggcgaag gcgacgttcg agatgatgta
     1081 tccgacgctc tccggcgaga taccggtgag gctgctgccc aggtccatca gccatggata
     1141 gagcggccag aaggctgctt tagcggggaa cgaccccatc ggctcgtacc cgctgacagc
     1201 gacaaggcga taccaggtgc catcccactg gcgcatcggc tccacaagcc agtgcgcgag
     1261 accagacatc gagccgagcg ggtcgccata ggcccagctc tcgccactgc gggtgccagg
     1321 agcgctcggg cggatggtgc cgtagaggaa cgtgagggta gccggaacct gggcgacgac
     1381 ccagtgcacc gcgaagacga ggaggggaaa ggtccagcgg tccgccggcg gtctgcgccg
     1441 ggagtagcgg cgtcgcgggg cggatatcgt ggtctggtcg gccgtttcgg tcaccatgca
     1501 gtactctcgg tggggctact ccggtcggtc acaggccgcg ggtccagcgg ggaccgcgac
     1561 gcacgcccaa tgatgccaca agccggtttg cagccttgcg ttgcccttgg catgcctgat
     1621 gcgtctacgc aacaatagca accaacggtt gagcagtcga atcatccgcc gcgatggtgg
     1681 aggagggtgt tggatgaacg cgaagtcgct gatcggcatg accgtgttcg cggtcgatgc
     1741 cggcaagaac ctgggttcgg tggatcgctt gctcttctcg actgagcgga tgcgggtgac
     1801 tggtctcgtc atcgcccccg ccaccggact catggatgag ccacgaccgc agaagctgct
     1861 cccgaccgac aaggtcaagg ccgtcgggca tgacgccatc acggtcgact cggagtcgct
     1921 gctcgaggtg ctcgccgatg gcgagctgcc ggcaggtgcc gtcgcgttcg atcagatcga
     1981 gcgcgagcga gtgctcaccg agagcggtga ggagatcggt gaggtctcct cggtggaatt
     2041 cgacgagctg acgttccagc tggactatct tgagatccgt cgggggttcc tgagcggcag
     2101 cagcatgatc gcggtggaga gcgtcgtatc ggtcggcgag gacgtcatcg tcgtacgcga
     2161 tacggcgctt gaggacgccg ctggcaggga cagggccgag gaggatcgcg tcgaacacga
     2221 ggatgacgac gggatgatca tcgagcgcag gtaacgactg cccgcagcga acgccgaaaa
     2281 atgagaaaga cccgaggctc ggcggtgaac cacgggtctt tcctgtgcgc acgtgacggg
     2341 tcgggtccac atcagtcaac cagcatggcg tgtgtatctc ccgcaccgac gttgacc
//
LOCUS       DASYWL010000275         2495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_408705, whole genome shotgun sequence.
ACCESSION   DASYWL010000275 DASYWL010000000
VERSION     DASYWL010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408705"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VGR22_09840"
     CDS             <1..401
                     /locus_tag="VGR22_09840"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV2128907.1"
                     /translation="IEKGAAMIDDEFYENHGAMLTRRQRQVLRLQAYGLTHQEIADYL
                     GLSIWTVKNHHKQAVRTLAASLRSVPRERVRTSLACYILGLLDGGWTPRDVAKHLKEV
                     SPPLQNPSPNNHPLGDVTEDELAPAAPAVQ"
     gene            complement(436..1359)
                     /locus_tag="VGR22_09845"
     CDS             complement(436..1359)
                     /locus_tag="VGR22_09845"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEV2128908.1"
                     /translation="MMQLMRQVVLGVADNQYVSRFFQTSRLTRSLVDRFVAGETLDEA
                     LVACRAIAASGMALTLDQLGENVHTREAAEVACDIYVDILCRLHAEGLERNISVKLTM
                     LGLDLGDEVAHDAMVRVLETAREVGGFVRIDMEGSAYTERTMRMFTKLHDRFPDEVGI
                     VIQAYLHRAEQDVRDMIARNARVRLVKGAYAEPASVAYQDSGKIDENYVRLMKLLLDE
                     GNFPALATHDPALIQAAQAHARERQIPLDRFEFQMLYGVRGDEQERLTKAGYGMRVYV
                     PYGTEWYPYFTRRIAERPANALFVLRQLVNR"
     gene            complement(1584..>2495)
                     /locus_tag="VGR22_09850"
     CDS             complement(1584..>2495)
                     /locus_tag="VGR22_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128909.1"
                     /translation="EYRVDAAYQTLPFRMKDVFMSAILVAANEALLRLASIADAILEQ
                     VEIIESWIERGRLGLAEQWNDERVMCCDRDLVAGRNVTVRTLASLAPVIAGGLTSERR
                     DALLEMWRSEAMVGHPNLRWPLPPSTSPLEPEFSPRTYWRGPVWPVMNWLFAWSLRRS
                     GAPNEPTSLPVPGWRRCAQSASRSMPSRSPGSRSARWTSRGRRLSRSTGSLTASMPDR
                     LRRCGRAIGIWLMAAEKTTWLCDSIGRFARRCGRIETARMLGRLGSLRKAIAAGFPVW
                     HGASALRTHRVGDNGAFWARRCDRAGT"
BASE COUNT          446 a          848 c          787 g          414 t
ORIGIN      
        1 gtatcgagaa gggagcagcc atgatcgacg acgagtttta cgagaatcac ggcgcgatgc
       61 tgactcgtcg gcagcgtcag gtgctgcggc tgcaggccta tggactgacc caccaggaga
      121 tcgcggacta tctcggtctc agcatctgga cggtgaagaa ccatcacaag caggccgtgc
      181 gcacgttggc cgcctcgctg cgatcggtgc cgcgcgagcg ggtgcgaacc tcactggcct
      241 gctacatcct tgggctactc gacggcgggt ggaccccgcg tgacgtcgcg aagcatctca
      301 aggaggtctc gccgccgctg cagaacccgt cgcccaacaa ccatccactc ggcgacgtca
      361 ccgaggacga gttggcgccg gcagcaccgg ccgtgcagtg accggatggc gccgaccgag
      421 tgggcgtctc cacatctagc ggttgacgag ctggcgcagc acgaacaacg cgttggcggg
      481 tcgctcggcg atccgccgcg tgaagtacgg gtaccactcc gtgccgtagg gaacgtagac
      541 ccgcatgccg tagccggcct ttgtcagccg ctcctgctca tccccgcgca cgccgtagag
      601 catttggaac tcgaaacggt ccagcgggat ttgccgctct cgggcgtgcg cctgagcggc
      661 ctggattagc gccgggtcgt gggtcgccag cgccgggaag ttcccttcat cgagcagcag
      721 cttcatcagc cggacatagt tctcgtcaat cttgccgcta tcctgataag ccaccgacgc
      781 cggctcggcg taggcgccct tgaccagccg cacccgcgcg ttgcgcgcga tcatgtcccg
      841 gacgtcctgc tccgcacggt ggagataggc ctggatgacg atccccacct cgtctgggaa
      901 gcggtcgtgc aatttggtga acatgcgcat ggtccgctcg gtgtaggctg agccctccat
      961 gtcgatccgg acaaagcctc cgacctcgcg cgcggtctcc agcacgcgca ccatcgcgtc
     1021 gtgggcaacc tcgtcgccca gatcgagccc gagcatggtc agcttgacgg agatgttgcg
     1081 ctccagaccc tctgcatgga gtcgacacag gatgtcgaca tagatatcgc acgcgacctc
     1141 ggccgcctca cgggtgtgca cgttctcgcc gagctggtcg agagtgaggg ccatgcccga
     1201 tgcggcgatg gcgcgacagg ccacgagggc ctcgtcaagc gtctcgccgg cgacgaatcg
     1261 gtcgaccagc gagcgtgtca gtctgctggt ctgaaagaag cgggagacgt actggttgtc
     1321 ggcgacgccg agcaccacct gccgcatcaa ctgcatcata accctccgcc gcaactctgc
     1381 gggaccggcg catcacgact gatgtacgtc tggattatcg caccgcgtgg cgcccccgta
     1441 gcggcttccg ccggccaacc tgccacccaa tcatcgacgg tccctcgccg ccaaggagca
     1501 atgtcatcct cagcggaggc tcgtgaggcc ggagttgaag gatctcttgt gaggtgggtc
     1561 gccaggttgg aggatgacat catctatgtg ccggcccggt cgcaccgcct cgcccagaat
     1621 gcgccattgt ctcccactcg atgcgttcgc aaggcagagg ctccgtgcca cacaggaaaa
     1681 ccagcagcga tcgccttgcg aaggctgccg agtcgcccga gcatcctggc agtctcgata
     1741 cgtccgcaac gacgggcaaa tcggccgata ctgtcacaca gccaggttgt tttctcggca
     1801 gccatcaacc agatgccgat cgcacgcccg catcgacgca gccgatcagg catcgacgcc
     1861 gtgagcgagc cagtcgagcg cgacagccgc cgtccacgac tggtccagcg agctgagcgg
     1921 ctccccggtg aacggctcgg catactccgc gaagccgatt gcgcgcacct gcgccaaccc
     1981 ggcacgggca agctggtcgg ctcgttcggc gcgccagacc ggcgtagcga ccaggcgaag
     2041 agccagttca tcactggcca gaccggccct cgccagtagg tgcgcggcga aaactccggc
     2101 tccagtgggc tggtgctcgg tgggagtggc catcgaaggt ttggatgtcc gaccatcgcc
     2161 tccgagcgcc acatctccag cagcgcatca cgccgctctg acgtgaggcc accggcaatc
     2221 acgggcgcga ggctcgccag cgtgcgcacc gtgacattcc gcccggcgac gaggtcgcgg
     2281 tcgcagcaca tcactcgctc gtcgttccat tgctctgcga ggccgagccg accccgctcg
     2341 atccagctct cgatgatctc cacctgctcg aggatcgcgt cggcgatgct cgccagccgc
     2401 agcagcgcct cgttggcggc caccaggatc gcgctcatga agacgtcctt catacggaac
     2461 ggaagcgtct ggtaggcggc gtcgacccgg tactc
//
LOCUS       DASYWL010000276         2778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_652336, whole genome shotgun sequence.
ACCESSION   DASYWL010000276 DASYWL010000000
VERSION     DASYWL010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652336"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1745)
                     /locus_tag="VGR22_09855"
     CDS             complement(<1..1745)
                     /locus_tag="VGR22_09855"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEV2128910.1"
                     /translation="MVPESVLETIKTVSTELHGRTLTLESGRIAEQAMGAVVVRYGDS
                     MVLSTVVGEREPSSLPFFPLTVDYEERMYAAGKIPGGFIKREGRPTERAILAARLTDR
                     PLRPLFPKGYRSEVQVITTVLSADQENDPALLSIIGASAALAISPIPWDGPVGAVRMG
                     MVEDELVVNPTFNEIEAGMLDMVVAGTDDAIMMVEGESDQIPEEKLLEGIERAHEAIR
                     EIVRIQNELVQAVGAEKWEFDAPEPNTELREDIKAYLGDALRSAVRQADKVLRVEAMG
                     DLEKRVIEHFTTGDDEDSLYTTSEVSGVYQELLKEEIRSGIVEGGIRPDGRRPDQIRP
                     IWIETGYLPRAHGSAIFTRGQTQVLTTVTLGSSAEEQRLDSISPEETKRYIHHYNFPP
                     YSVGEVRRMRGASRRDIGHGALAERALITVVPNEDEFPYTMRLVSEVVSSNGSTSMAS
                     VCGSSLALMDAGVPISDPVAGVAMGLVIDKETGSFTVLTDIQGVEDAYGDMDFKVAGT
                     AEGVTAIQMDIKVKGITVEIMRQALQQANTGRQHILGKMTEAIAAPRAELAKHAPRVT
                     RVKVNPDKIGAIIGPG"
     gene            complement(2059..2328)
                     /gene="rpsO"
                     /locus_tag="VGR22_09860"
     CDS             complement(2059..2328)
                     /gene="rpsO"
                     /locus_tag="VGR22_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEV2128911.1"
                     /translation="MALQKEQKTDIIEQTRTHDGDTGSPEVQIALLTERINGLAAHLR
                     AHRHDHHTRRGLLKLVGRRRRLLAYLSKNDLERYRAVIARHGLRR"
     gene            2566..>2778
                     /locus_tag="VGR22_09865"
     CDS             2566..>2778
                     /locus_tag="VGR22_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEV2128912.1"
                     /translation="MLGVIGGSGLYDLPGLQNVQEHRPQTPFGEPSAPILQGMLGALD
                     LCFLPRHGKGHAHNPSEVPYRANVYAL"
BASE COUNT          445 a          944 c          868 g          521 t
ORIGIN      
        1 cccgggccga tgatcgcgcc gatcttgtcc ggattgacct tgacgcgagt cacccgcggc
       61 gcatgcttgg cgagctctgc ccgcggcgcg gcgatcgcct cggtcatctt gccgaggatg
      121 tgctgtcggc cggtgttggc ctgctgcagc gcctgccgca tgatctcgac cgtgatgccc
      181 ttgaccttga tgtccatctg gatcgccgtc acgccctcgg cagttccggc caccttgaag
      241 tccatgtcac cgtaggcgtc ctccacgccc tggatatcgg tcaggacggt aaaggatccg
      301 gtttccttgt cgatcacgag gcccatcgcg acacctgcga ccgggtcgct gatcggcacg
      361 ccggcatcca tgagcgccag cgaggagccg cagacgctgg ccatcgaggt cgagccgttc
      421 gagctcacga cctcggagac gagccgcatc gtgtacggaa actcgtcctc gttcgggacg
      481 acggtgatga gcgcgcgctc ggcaagggcc ccgtgtccga tgtcacggcg gctggcaccg
      541 cgcatccgac ggacctcccc cacactgtac ggcgggaaat tgtagtggtg aatgtaccgc
      601 ttcgtctctt ccggactgat actgtccagc cgctgctctt cggcgctcga gccaagggtg
      661 accgtggtga gcacctgcgt ctggcctcgc gtgaagatcg cagagccgtg cgcgcgcggc
      721 aggtagccgg tctcgatcca gatcgggcgg atctggtccg gacggcgacc gtccggacgg
      781 attcccccct cgacgatgcc gctgcggatc tcctccttca gcaactcttg atagacgccg
      841 gagacctctg aggtcgtgta gagcgagtct tcatcgtcgc ccgtcgtgaa gtgctcgatg
      901 acccgcttct cgaggtcacc catcgcttcc acgcggagca ccttgtcggc ttggcggaca
      961 gcagagcgaa gcgcatcccc gaggtacgcc ttgatatcct cccggagctc ggtgttcggc
     1021 tccggagcgt cgaactccca cttctcggca ccgacagctt gcaccagctc gttctggatc
     1081 cggacgatct cgcggatcgc ctcgtgcgcc cgctcaatgc cctcgaggag cttttcctcg
     1141 gggatctggt cggactcgcc ctccaccatc atgatggcgt catccgtgcc ggcgaccacc
     1201 atgtcgagca tgccagcctc gatctcgttg aaggtcgggt tgaccaccag ctcgtcctcg
     1261 accatgccca tgcgcacagc gccaaccggg ccgtcccacg ggatcggcga gatcgccagc
     1321 gccgccgatg caccgatgat cgagagcagc gccggatcgt tctcttggtc ggcggagagg
     1381 acggtcgtga tgacctgcac ctccgaccgg tagcccttcg ggaagagtgg tcgcagcggc
     1441 cggtccgtga gccgcgctgc gaggatggcg cgctccgtcg gccgcccctc gcgcttgata
     1501 aatccacccg ggatcttacc ggcggcatac atccgctctt cgtagtccac cgtgagcggg
     1561 aagaatggca ggctcgacgg ctcgcgctcg cctaccactg tggagagcac catcgaatcg
     1621 ccgtagcgta cgacaaccgc gcccatcgcc tgctcggcga tccggccgct ctccagcgtc
     1681 agtgtccggc cgtgaagctc cgtgctgacg gtcttgattg tttcgagtac ggattctgga
     1741 accatgtgta tcctccagac cttgcgtctg ttgattcgac aggaccggcc ggcgccggtg
     1801 tctcgacgct ggcgatccct gatcatggca agggcggaag gtcttgaggg actggcgcag
     1861 ggcgacgcac ccgacgcctc gcactgcccc aatacctcaa aagattccgc ccgccggcgg
     1921 gtgaaacgcc tcctctctcg atacggcaaa tgcccagaat cccggacgga tcctgggcat
     1981 tgctctcacc gttcgcccgt gcagccgaag acctccgcac tcattgtctg gatccggaag
     2041 ccaccgacca ttcggccact agcggcgcag gccgtgccga gcgatgacgg cgcggtagcg
     2101 ctcgagatcg ttcttgctca ggtaggccag cagccggcgg cggcggccga cgagtttgag
     2161 cagaccacga cgggtgtgat gatcgtgccg gtgcgcgcgc aggtgggcgg cgaggccgtt
     2221 gatacgctcg gtcagcagcg cgatctgcac ttccggcgag ccggtgtccc catcatgggt
     2281 ccgggtctgc tcgatgatgt cggtcttctg ttccttctgc aatgccatgc ttcgggcgcc
     2341 tcctcggaga gtgcctgatc gtgctcaggc cgatgtcgtc aggttcagtg tttgcacaca
     2401 cagaaaaacg cccgtggcaa cggcgcttac cgagcgagta taccacgtcg gcccgaacgg
     2461 ttgtcacccc gcctcgctgc cggcaggtgc cgggtacact tggcacagcg tcacggtccg
     2521 cctgcggcac ggcgggctct cgtactgccc acgaaaggga ttcgcatgct cggagtgatc
     2581 ggtggtagcg gcctctatga cctccccggt ctgcagaatg tccaggaaca tcgccctcag
     2641 acgccattcg gggagcccag cgcgccgatc ctgcagggca tgctcggtgc actcgacctc
     2701 tgcttcttgc cacgccacgg caagggccac gcccacaacc cgagtgaggt gccgtaccgc
     2761 gccaacgtct atgccctg
//
LOCUS       DASYWL010000277         3330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_615035, whole genome shotgun sequence.
ACCESSION   DASYWL010000277 DASYWL010000000
VERSION     DASYWL010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615035"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..302
                     /locus_tag="VGR22_09870"
     CDS             60..302
                     /locus_tag="VGR22_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128913.1"
                     /translation="MAKLVYVGTHGPDDPTKACMPIHLAVNGAAEAGIETEINLAGDA
                     AVLIQDTITDAVLPIGFPPLKDLLAKAREKGVHVFI"
     gene            complement(548..1450)
                     /locus_tag="VGR22_09875"
     CDS             complement(548..1450)
                     /locus_tag="VGR22_09875"
                     /inference="COORDINATES: protein motif:HMM:NF019307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HEV2128914.1"
                     /translation="MKAVRGEPPLGVKFNIEGEEEISSPSVIDFALEHRGLCQADACI
                     WEFGGRDIAGRPQVHLGLKGICYVELRVKGARLDWHSSVAASVPNPARRLIWALNAIK
                     GEDGRVKIPGFYDSIKPPTQEEIEALEQMPNYEAKRLEYLGIEEFPDGRTSLDLNLQD
                     YFQPTCTVSGFVSGYTGEGAKTVLHSRAMAKLDMRLVADQDPFNVYESLRALLDENGF
                     DDIETELLGPSTRHSLQSTRRSSRSLRRRTPTSTGRSLWLVYATSQGSSGPWYQLCGQ
                     FGIDACTAGVGHAKSRAHAPNENI"
     gene            complement(1586..1843)
                     /locus_tag="VGR22_09880"
     CDS             complement(1586..1843)
                     /locus_tag="VGR22_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128915.1"
                     /translation="MSEDLRNIYAYIDEHADESIEELQRLLRQPSVAAQNHGMRETAD
                     MVEEMVRGVGFDPRQVPTAGFPVVYAEQRGKSDKTLSFYNH"
     gene            complement(1850..3082)
                     /locus_tag="VGR22_09885"
     CDS             complement(1850..3082)
                     /locus_tag="VGR22_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEV2128916.1"
                     /translation="MTKTTDSARFRELEERVLAQIDQEEVIAFHRSLVQERTVNPPGD
                     VREAFAICEETVRKDGFATKQVGDHDLMPNLVAIWGDAGGPTLCFNSHYDVVPTGEES
                     AWNHDPWAAGIVEGRIYGRGAGDAKASVAAQVMAGIAVARAGIPVRGALVVNEVADEE
                     VGGVHGAELIVREGHVRPDWVIIGEQTGNRVAVGEKGAAGTEVMLHGRTAHGALPWEG
                     ANAIEAMAEVIVALRRELWPQLGNRTHEFFHPSSASVNLLEGGVKANVVPDHASFFID
                     RRIVPGEQPEECVAEIRRIAEAAVAEMPGIRVEVRPAGKGWESNPVSVQDPLVQSMVG
                     ANARLGLSTEPTGFSMGTDGRFFAAAGYPTIIYGPGDPATAHVPDEWVGIDEIMDATR
                     AYAIAAVRLIGKHEEEKG"
BASE COUNT          596 a         1092 c         1025 g          617 t
ORIGIN      
        1 acagttcgct tacggtacgg ctcccgccta ccgggaggct acgatgcagg gaggcggcaa
       61 tggcgaagct cgtctatgta ggcacgcatg gtccggacga tccgaccaag gcgtgcatgc
      121 ctatccactt ggcggtgaac ggtgcggctg aagcgggaat cgagacggaa atcaacctgg
      181 caggtgacgc agcagtcctg attcaggata caatcaccga tgcggtgctt ccgataggtt
      241 ttccgccgct gaaggatttg ctggcgaaag cgagggagaa gggcgtccac gttttcatat
      301 gaggtggctg tgcgcagccc cgtggggtca gcgacgccga tatcgaaggc aagaacgcat
      361 ccttctcgac gccaaccgag ttcgcgcagc gttgcgccga agccaccaac gtcctggtgt
      421 tctagcttgc ggcgacccgg gcggcgcggg tgatggccgt ccgatcgccc ttctatccct
      481 cccccacccg cgcgaagcgg cccacaatcg cgcagatgtg cttgatgccc aagatgaagt
      541 cgtcgactca gatgttctcg ttcggcgcat gcgcccgcga ctttgcgtgc ccgacaccgg
      601 cagtgcacgc gtcgatgccg aactgaccgc agagctggta ccacggccct gaagaaccct
      661 gtgacgtcgc gtagaccaac cacaggctcc tgccagtaga ggtcggtgta cgtctcctca
      721 aggaccttga cgatcggcgc gtcgactgga gcgagtgacg ggtactgggc ccgagcaact
      781 cggtctcgat gtcgtcgaaa ccgttctcgt cgagaagggc ccgcagcgac tcataaacgt
      841 tgaatggatc ctgatcggcg accagccgca tgtcgagttt ggccatggcg cgtgagtgta
      901 gcaccgtctt ggcgccctcg ccggtgtacc cgctcacgaa gccggacacg gtacacgtcg
      961 gctggaagta gtcttggaga ttgagatcta ggctagtgcg tccatcgggg aactcctcga
     1021 ttccaagata ctcgagccgc ttcgcttcgt agttgggcat ctgttccagc gcctcgatct
     1081 cctcctgggt aggtggcttg atactgtcgt agaatcccgg gattttgacg cgcccgtcct
     1141 cgcccttgat cgcgttcaat gcccaaatga ggcgccgagc cgggttcggt acgctagctg
     1201 ccaccgagga gtgccagtca agccgtgcac cctttacacg cagctcgacg tagcagatgc
     1261 ccttgaggcc gaggtgaacc tgcgggcgac cggcgatgtc acgcccgccg aactcccaaa
     1321 tgcaggcgtc cgcctggcag agtcccctgt gttcgagcgc gaagtcgatc acgctcggtg
     1381 agctgatttc ctcctcaccc tcgatgttga acttgacgcc cagcggcggc tcgccccgaa
     1441 cggccttcaa gcgcggatcg ccgagacgcg cgctgcaatg ttgcccttat tgtctgcgac
     1501 gccacgggcg tagatgcgcc cgtggcgcat atccgcccca gggatccgaa ccccactcgt
     1561 ccagcgggtc agctggttgc acatcctagt ggttgtagaa gctcagcgtt ttgtcggact
     1621 tgccgcgctg ttccgcgtag acaaccggga agccggcagt gggtacctgg cgcggatcga
     1681 agccgacgcc gcggaccatc tcttcgacca tgtcggcggt ctccctcatc ccgtggttct
     1741 gtgccgcgac actcggctgc cgcagcaggc gctgcagctc ttcgatcgat tcgtccgcat
     1801 gttcatcgat gtaggcgtag atgttccgca gatcctcgct cacgaatcct taccctttct
     1861 cttcctcatg cttgccgatc agacggaccg ccgcgatggc gtaggcccgg gtggcgtcca
     1921 tgatctcgtc gatgccgacc cactcgtcgg ggacgtgcgc cgtcgcggga tcacccggac
     1981 catagatgat cgtcggatag ccggcagctg cgaagaatcg gccgtcagtt cccatgctga
     2041 agccggtcgg ctccgtcgaa agcccgagac gcgcgttcgc gcccaccatc gactgcacca
     2101 gcgggtcctg gacggacacg ggattggact cccacccctt cccggcagga cgcacctcca
     2161 cgcgtatgcc tggcatctcg gccacggccg cctcggcaat ccgccgaatc tcagcgacgc
     2221 actcctccgg ctgctcaccc ggcacgatgc gccggtcaat gaagaacgag gcgtgatccg
     2281 gcaccacgtt ggccttgacg ccgccctcca gaaggttgac ggatgccgac gacgggtgaa
     2341 agaactcgtg ggtgcgattc ccgagctgtg gccagagctc gcgacgcagg gcaacgatca
     2401 cctcggccat cgcctcgatc gcgttggcgc cctcccatgg caacgcgccg tgcgccgtcc
     2461 ggccgtggag catcacctcc gtgccggccg ctcccttttc accgaccgcc acccggttac
     2521 ccgtctgctc accgatgatg acccagtcag ggcggacatg tccctcgcga acgatgagct
     2581 ccgcgccgtg gacgccacca acctcctcgt cggcaacctc attcacgacc agagcgcccc
     2641 ggacaggaat gcccgctcgg gccacggcaa tgcccgccat cacctgtgcc gcgacgctcg
     2701 ccttggcgtc tccggcgccc cgcccgtaga tccggccttc aacgatcccc gccgcccagg
     2761 ggtcgtgatt ccaggccgac tcctcgccgg ttggcacaac atcgtagtga gaattgaagc
     2821 agagcgtcgg cccgccggcg tctccccaga ttgccaccag attcggcatg aggtcgtggt
     2881 cgccgacctg tttggtcgcg aacccgtcct tgcgcaccgt ttcctcgcag attgcgaatg
     2941 cttcccgcac gtcgccggga gggttgacgg ttcgctcctg tacgagcgag cgatgaaagg
     3001 cgatcacctc ttcctggtcg atctgagcca gcacccgctc ctcgagctcg cgaaaccgcg
     3061 cggagtctgt tgtcttggtc atcattccct cttgtgcatt aaggacggtg gcaggcgaga
     3121 tgcgggtatc gagataggag agggacggtc atggggatcg ccccgaggaa ctgcagaagg
     3181 aggatgcagc agatggacaa ttctgggaag aaggtagcga tggttctcgc acccaacttc
     3241 gaggatgtcg aggcgaccga cccgaagcag gcgcttgagc aggccggggc gcaggtcacc
     3301 atcatcggga tcgagaaggg cgagatcgag
//
LOCUS       DASYWL010000278         6241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_183434, whole genome shotgun sequence.
ACCESSION   DASYWL010000278 DASYWL010000000
VERSION     DASYWL010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6241
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183434"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VGR22_09890"
     CDS             <1..529
                     /locus_tag="VGR22_09890"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2128917.1"
                     /translation="AGWSYGAHVTLAFALEYPERIRTLTLIEPPAVWIMRETGWNPPE
                     RARIEAFDRGMTGRELTADDLKAFLVRAGFGPPETDFEALPTWPSWLAHRQALAINGT
                     IWDYRDTLDRLRELRVPVLAILRTESPDSFKSIVETIVTTAPSATLLELPGNHACHLQ
                     NPDRLLFELEAHLDT"
     gene            complement(699..1193)
                     /locus_tag="VGR22_09895"
     CDS             complement(699..1193)
                     /locus_tag="VGR22_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128918.1"
                     /translation="MISRFVLLLSILAAMFVSIQATSAQGEDQYTAAIVSGRCIDIGE
                     AVLVLDTPQYETGEWRGTEGAPVVLESDNDDIGEVPASVLFDTPHSVVVLEDDDPVAC
                     GEFGGLVGVEEDQDLIFGLSPVNDSGYVGVVVVNGVSRDDEDDEFEVDVYVIDPESLA
                     IDEG"
     gene            1449..2723
                     /locus_tag="VGR22_09900"
     CDS             1449..2723
                     /locus_tag="VGR22_09900"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2128919.1"
                     /translation="MSVAGSLSRHLYYGWVQVLTLSVTETISWGILYYAFSVFILPME
                     ADLGWTRSQITGAFSLALIVSGIAGIPIGRWLDRHGPRALMTAGSVAATLLVLVWSQV
                     GNLTTWYLLWVGIGVTMAATFYEPAFAMIAVWFRRKRSRALTILTFFGGFASVIFIPL
                     SERLVQAFDWRTALVILAAILAVGTIPAHALLLRRHPADLGLRPDGELAPEPSLNRSP
                     ATADNDDEPALTWQDALRGATFWWIGGAFMLVMLAMVAVTVHLIPTLVNRGFSSGFAA
                     SAAGLVGLFALPGRLVFTPLGGRLPRQVVTALIFALEAAAIAVLAIADTRAEVIAFVV
                     LFGSGFGAITPARAALVADVYGSRSFGTIAGVLAMLVTTARGAGPILASLLIAWWDGP
                     TGMLWIMTALCAVGALAILFSGTGQPGRAQTR"
     gene            complement(2927..4609)
                     /locus_tag="VGR22_09905"
     CDS             complement(2927..4609)
                     /locus_tag="VGR22_09905"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEV2128920.1"
                     /translation="MPWATRLGRLIPRLLLVLALALLVAAIALFTFRAIYVDKVYPAI
                     AVGDVSVGGMTVEQAVATVEQRAAELERGTVTFTYNGQTWTPTLAELGASIDVESSVD
                     AAWELGRDESAVDRLGFTRQLLHGDQRVPLRTMVDLTVLAGWFEQVNADIDQRAVNAD
                     MVVDGTEVSISPEKSGIIVDEDAATELILGALESLEPVSTEMPTTTEVPKIFADDLEG
                     LRSDLAIRLDDPMVAVFEGQEWEIPPAELTEFLTVETTYENGATRVDVGFERDGLTAY
                     LRNTFSGKVNRSPVDARIAWSAADGGLISLEQSVDGAALRSNAFADAVAESFLSDQAL
                     IEIPAVVTKPEVDSNNLGALGIDGRISRATSNFAGGSESRDTNISVGTRLLNGELIAP
                     GEEFSFNGAVGEITYDKGFVDGSVIEAGIIGRSVGGGICQVTTTVFRAALLSGMPITE
                     WWPHSLRLLGYERNGWTAGYDASILQSGSDPALWADFKFKNDTDGYMIVHAWNEYPYN
                     IVEIYGSDDGRTMEVGDAQFSTPNGKYAAWFTRMITYPDGTRKECTFESVYK"
     gene            5409..5732
                     /locus_tag="VGR22_09910"
     CDS             5409..5732
                     /locus_tag="VGR22_09910"
                     /inference="COORDINATES: protein motif:HMM:NF013089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEV2128921.1"
                     /translation="MGFSRHRHRRGGRRHTALRYSNGFTVVLPSIVTVAGYALAFFML
                     SQALRTVDLGIAYAVWAGIGTAAIALISMVVFGEPGTMGKMWKTIGLVLIIAGVVLVN
                     LQGAH"
     gene            6006..>6241
                     /locus_tag="VGR22_09915"
     CDS             6006..>6241
                     /locus_tag="VGR22_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128922.1"
                     /translation="MSTRPTSHPDWQDPAITNRNREPARPLLIPYADDASALTGDRTQ
                     SPWYRSLNGDWLFHYAESVELVPPGAADPDTDDS"
BASE COUNT         1051 a         2235 c         1799 g         1156 t
ORIGIN      
        1 cgcgggctgg tcctatggcg cgcacgtcac cctcgcattc gcgctggagt atccggaacg
       61 tatccgcacc ctcaccctca tcgagccgcc cgctgtctgg atcatgcgcg agaccggttg
      121 gaacccgcca gaacgggccc ggattgaggc attcgaccgt gggatgacgg gtcgcgagct
      181 cactgccgac gacctcaagg cgtttctggt tcgcgccggg ttcggcccgc cggaaaccga
      241 cttcgaggcg ctgcccacgt ggccgtcatg gctcgcgcac cgccaggcac tggcgatcaa
      301 cggcacgatc tgggactaca gggacacgct cgatcgtctg cgcgagctgc gcgtgcccgt
      361 gctcgccata ctcaggaccg agagccctga cagcttcaag agtatcgtgg agacgatcgt
      421 gacgaccgcc ccgagcgcga cgctgctcga gcttcccggc aaccacgcct gccaccttca
      481 gaatcccgac cgcctccttt tcgagctgga agcccacctc gacacctgat gatctgcccc
      541 tcgatgtgag tcgacagtca gcacgcgacg accgccggtt gatgtgccat caaccggcgg
      601 tcatgcagag gcacatcacc atgccttgaa caggcatttc tcggcgatgt gcctgaaacg
      661 ctgacacacg caatcgccct gcggcggtcg gcgaccgctt acccttcgtc gatagccaaa
      721 ctctccgggt cgatgacgta cacgtcaacc tcgaactcgt catcttcatc atcgcggctg
      781 accccattga caaccacaac accgacgtac cctgagtcat tgacgggcga caggccgaaa
      841 ataaggtctt ggtcctcttc aacgccgacc agcccaccaa actctccaca ggcaaccggg
      901 tcatcgtctt caagcacgac gaccgaatgt ggtgtgtcga aaagcacgct ggcagggact
      961 tcgccgatgt cgtcgttgtc cgattccagc accacgggcg cgccttccgt gcctctccac
     1021 tcgcccgttt cgtactgcgg cgtgtcgagc acgaggactg cttcaccaat atcgatacat
     1081 cgtccactga cgattgcggc agtgtactgg tcttcgcctt gggcagaagt cgcctggatc
     1141 gagacgaaca ttgcggccag gatggacagg aggagaacga accgtgagat catcaggcaa
     1201 ctccctgcgc tacgccaata cactcaacac gccgcccctg cacggatggc gtggcattgg
     1261 tggggtgagc ggtgcaagaa ccgttccatc tatggagtgg ctccgcacac gaaagctggc
     1321 ggagccactc ctggacgacg ccgatcgtct cccccgccgt cagcgccgcc gcgtctcgcc
     1381 attgccaccc gccactcgcc ctgcgatcat gccgctagac tgccacgatc caatcgctgg
     1441 agcgcttcat gtccgttgcc ggtagcctgt cccgccacct ctattacggc tgggttcagg
     1501 tgctgaccct ctcggtcacc gagacgatat cgtggggcat cctctactac gccttctcgg
     1561 tcttcatcct gccgatggag gccgatctgg gctggacccg cagccagatc accggcgcct
     1621 tctcactggc actgatcgtc tccggcatcg ccggcatccc cataggccgc tggctcgacc
     1681 gccacggccc ccgcgcgctg atgaccgccg gctcggtcgc cgcgacactg ctcgtgctgg
     1741 tctggtccca ggtgggaaac ctcaccacct ggtacctgct ctgggtcggc atcggcgtca
     1801 ccatggcagc gaccttctac gagcccgcct tcgcaatgat cgccgtctgg ttccgccgca
     1861 agcgcagtcg cgcgctgacc atcctcacct tcttcggtgg cttcgccagc gtcatcttca
     1921 tccccctctc cgagcgcctc gtgcaggcgt tcgactggcg cacagcgctg gtgatccttg
     1981 ccgcgatcct cgcggtcggc accattcccg cccacgccct gctgctccgt cgccaccccg
     2041 ccgacctcgg tctccgcccg gacggcgaac tcgctcccga gccatcattg aaccgcagcc
     2101 ccgcgaccgc cgacaatgac gacgagcctg ctctcacctg gcaggacgcc ctgcgcggcg
     2161 cgaccttctg gtggatcggc ggagccttca tgctggtgat gctggcgatg gtcgccgtca
     2221 ccgtccatct cattccaacg ctggtcaacc ggggcttcag cagcgggttc gccgccagcg
     2281 cggccgggct ggtcggcctc ttcgcgctgc cggggcgcct cgtcttcacc ccgctcggcg
     2341 gcaggctccc ccggcaagtc gttaccgccc tcatcttcgc gctcgaggcc gccgcgatcg
     2401 ccgtgctcgc catcgccgac acccgcgccg aggtgatcgc cttcgtggtg ctctttggct
     2461 cgggcttcgg cgcgatcacc cctgcgcgcg cggcgctggt cgccgacgtc tacggctcac
     2521 gctccttcgg caccatcgcc ggggtgctcg cgatgctcgt caccaccgca cgcggggcag
     2581 gcccgatcct cgccagtctg ctcatcgcct ggtgggatgg ccccaccggt atgctctgga
     2641 tcatgaccgc tctctgcgcc gtcggcgccc tcgccatcct cttcagcggc accgggcagc
     2701 caggccgcgc ccaaacgcga taggcaccgt cgataccacg tcatttcgac tggagtaccc
     2761 gaaaggcgtg gaatggcgaa atctcttgcc tagtgagggc cgatatccat cgcgcaagag
     2821 atttctcaac tccgcgacta ctccgcggag tctgccctgg gcgagcgctg caagcccgag
     2881 tcgaatgggc tcgaattgac ggtcggtgcc gacatttcta tctcgcctac ttgtacacgc
     2941 tctcgaaggt gcactcctta cgcgtgccat cggggtaggt gatcatgcgg gtgaaccagg
     3001 cggcgtactt gccattcggt gtcgagaact gcgcgtcgcc cacctccatg gtgcggccat
     3061 cgtcgctgcc gtagatctcg acgatgttgt acgggtactc gttccaggca tggacgatca
     3121 tgtacccgtc ggtatcgttc ttgaacttga aatcggccca cagcgccgga tccgagccgg
     3181 actgcaggat cgaggcatcg tagccggcgg tccacccgtt gcgctcatac ccgagcagac
     3241 gcaggctgtg cggccaccac tcggtgatcg gcattcccga gagcagcgcg gcccggaaaa
     3301 cggtcgtcgt gacctggcag atgccgccac caacgctgcg cccgatgata ccggcctcaa
     3361 tcacgctgcc gtcgacgaag cctttgtcgt aggtgatctc gccgaccgcg ccgttgaagg
     3421 agaattcctc gccgggggcg atcagttcac cgttgagcaa tctagtgccg acgctgatgt
     3481 tcgtgtcgcg tgattccgag ccgcccgcga agttggacgt cgcccgtgag atgcggccat
     3541 cgatgccgag cgcgccgagg ttattggagt cgacctccgg cttggtgacg acggccggga
     3601 tctctatcag tgcctggtcg ctcaggaaac tctcggcgac cgcgtcggcg aacgcgttcg
     3661 accgcagcgc ggcgccatcg acgctttgct cgagggagat cagcccgcca tcggcagcac
     3721 tccaagcgat gcgggcgtcg accggtgacc ggttgacctt accggaaaac gtgttccgca
     3781 ggtaggcggt gagcccgtcc cgctcgaacc cgacgtcgac acgcgtcgcg ccgttctcgt
     3841 aggtggtctc gacggttagg aactcggtca gctcggcggg cgggatttcc cactcctgac
     3901 cctcgaacac cgccaccatc gggtcgtcca gccttatcgc gagatcgctc cgaagtccct
     3961 cgagatcatc ggcaaagatc ttcggcactt cggtcgtcgt cggcatctcg gtcgacaccg
     4021 gctccagcga ctccagcgcg ccaaggatca gctcggttgc cgcgtcctcg tcgacaatga
     4081 tgccgctctt ctcgggcgag atgctgacct cggtgccatc cacgaccata tccgcattca
     4141 ccgcgcgctg gtcgatatcg gcgttgacct gttcgaacca gcccgccaat acggtaaggt
     4201 cgaccatggt gcgcagcggt acccgctggt cgccatggag aagctgtcgg gtgaatccga
     4261 gacgatcgac tgcactctcg tcgcgcccga gctcccaggc ggcgtcaacc gacgactcca
     4321 cgtcaatcga tgctccgagc tcggccagcg tcggagtcca tgtctgcccg ttgtaggtga
     4381 aggtgacggt gccgcgctcg agctcggcag cgcgctgctc gacggtggcg accgcctgct
     4441 caacggtcat gccgccgaca ctgacgtcgc cgacagcgat ggcggggtag accttatcga
     4501 catagatggc acggaacgtg aagagcgcga tcgccgccac aagcagcgcc aaggcgagca
     4561 ccagcagcag cctcgggatc agccgcccca gacgggtcgc ccacggcatc ccctcatcca
     4621 gtccatcact gcgatgcgag gccattccga acgaccacga caccttcgca ggcatcacgg
     4681 tgtcgcgcag gaagcctgtt gttcgcccga acctggacgt cgtgtccgcc gcatcgcgcg
     4741 ccccagccgt cccaggcaat gttcgttgca ccatcgatct gctttcttcg cctgttctcg
     4801 cgagcgcccc gtggcgactg accaacagca aaacgcctcc cctacacgtc cgattactcg
     4861 ccgtgttttc gagacgcgaa cgcccgctcc cacccttgcg gttccgtcag tttgcgttgc
     4921 ggaatgatgg tggctcgcgc tgccctccga agaccagacg cacgaccatc gctatcgggt
     4981 caaggatacc attccagcca gccattacag catcgtactc acgcccttct ccggctggat
     5041 ccgtcccctg tcctctcggt ttcgtcgatg gtacccgcgg ctcgagaagt cccgcaccgt
     5101 tgctggcaat agcggccgct ctctcatgac tatacgcacg cttgcgcgat ccggttgcct
     5161 ggccgcacga gcctctcgtg tccaccgaat gctccctcgc acgtccgatg ccgcgctctg
     5221 cgacaacgcg aagctgtgac agcaggtcca tacccatggg tcttgcctga tccggcattc
     5281 catcaccggc cacgcccgac agctcgcacc tctggttgga ccgatgatgc cgaccccctc
     5341 tggtacatcc gtacaggttt tgctcgtccg cgcgtgttgc gttcccacac agaggtaacc
     5401 atgatgggat gggtttttct cgccatcgcc atcgccgcgg aggtcgccgc cacacggcac
     5461 tgcgctactc caacggcttc accgtcgtgc tgccctcgat cgtgaccgtc gccggctacg
     5521 ccctcgcctt cttcatgctc tcccaggcgc tgcgcacagt cgatctcggc atcgcctacg
     5581 ccgtctgggc aggcatcggc acggcggcga tcgccctgat cagcatggtc gtgttcggcg
     5641 agccggggac aatggggaaa atgtggaaga cgatcggtct ggtgctgatc atcgccggcg
     5701 tggtgctggt caacctccag ggtgcccact gacatcgcgg gagcgccaat ccgtgccatc
     5761 aatagccaac ctgtcatttc gaccggagca cacgtcgtat gcggggtgca gaaacttctg
     5821 ccgcacatac cctaggcatc cggtttgcag cacccttctg cgctccacct tcgctaccct
     5881 cggactgcgc gcgaagcgac aggatgactt cccctcgccc gccgttcgtg gggaacgaat
     5941 cgtgcctcgc acggctacaa taccgagaaa tcgtgactga ccgcgcgaaa ggacaccccc
     6001 tccacatgag cacccgcccg acctcgcacc ccgactggca ggaccccgcc atcaccaacc
     6061 gcaaccgcga gccggcccgc cccctgctga tcccctacgc cgatgacgcc agcgccctga
     6121 ccggtgaccg cacgcaatcg ccgtggtacc gatcgctcaa cggtgactgg ctcttccact
     6181 acgccgagtc cgtcgagctc gtgcctcccg gcgcggccga tcccgatact gacgacagcg
     6241 a
//
LOCUS       DASYWL010000279         3331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_333503, whole genome shotgun sequence.
ACCESSION   DASYWL010000279 DASYWL010000000
VERSION     DASYWL010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333503"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VGR22_09920"
     CDS             complement(<1..206)
                     /locus_tag="VGR22_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128923.1"
                     /translation="MIRLLDRLRPSPTPQWLEPDSPPGDAPLTDIHESGLLARVLWHR
                     GIRDGAAARAFLTSGPAAAPSPWGL"
     gene            complement(203..664)
                     /locus_tag="VGR22_09925"
     CDS             complement(203..664)
                     /locus_tag="VGR22_09925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV2128924.1"
                     /translation="MPLPYDPTCTFCRITSGNVDRGNVFWEDDIALGPLDHRPLKPGH
                     ALLIPKGHYATMQHLPADLIGPLFQRAAILNRAVEVAFGADGSFSGYNTNVSQSIGHF
                     HLHVIPRQFGDKLFSGGKWIRQPVDDIDQHYARRDALGSVMRDLLVETGVV"
     gene            complement(885..>3331)
                     /locus_tag="VGR22_09930"
     CDS             complement(885..>3331)
                     /locus_tag="VGR22_09930"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HEV2128925.1"
                     /translation="LPQDALAACDTACAVIASGLERIRKRYPPIGKLLLSGHAHIDLA
                     WLWPVAETRRKVRRTWSSVLRLMDKYPDFTFNQSSAQAYKWMEEDDPELLERIKTRVA
                     EGRWEPIGGSWAEPDCQVTGGEAFARQLFYGQRFFEQTFGQRNTVAWLPDVFGFSGGL
                     PQLLQQAAITGFFTTKLIWNENNAFPYDLFWWQGLDGSRVLAHTFLNPNGGYNGHINP
                     LDTYQTWQNFIGKRIHDESLLAFGWGDGGGGPSEDMLQNYDRIKDYPVLPKLEMGMVE
                     QFYDSLPREGLPTWVGEMYLELHRATLTTQALAKQLNRQGEHRLVEAEAFATLASLDG
                     ADYPKAELDAAWQDLLYNQFHDILPGSSIREVYRDTHPQLLEVIATAITVRDEAIAAR
                     TGSGMDTFAITNPDLNARPLTALLPEGSDAGNLPSQRVEDGVLVHAPATTLGGFASII
                     VSSAAEDASKTGGVSATLRDSGAVTLKNGELTVEVGADGTLHRVFDRRVNREALSDRG
                     NQLWAYVDRPRAWDAWDIDETYENAGMEITDVDSMEMIEEGPHRAAVRVTRSWRSSRF
                     VQTYRLLSGSSRIDIDTQIDWHERLMLIRALFPTGVQSHEATFETMFGVQRRATHQNT
                     SWERARFEAGAHRFVDLSEPAYGVALLNDAKYGHSAHGSTLGVSLVRGPLHPDPFADE
                     GEHRFTYSLFPHIGDWVDGGVTREARALNAPLVVTAAASDATGREAFVASTGIELGLG
                     ALKRAHDQDGIVLRVYEPHGARGQAQLTFTETPASVTRVTILEEPQKEREVTLNGNTA
                     AFEIGPFEVVSLLIGA"
BASE COUNT          518 a         1041 c         1158 g          614 t
ORIGIN      
        1 aggccccacg gcgagggtgc cgctgccggg ccgctggtga ggaaggcgcg ggcagccgcg
       61 ccgtcgcgga tgcctcgatg ccagagcacg cgagcgagca acccgctctc gtggatgtcg
      121 gtgagcggtg catcgccggg cgggctgtcc ggctcgagcc actgtggagt cggcgagggc
      181 cgcaatctgt cgaggaggcg gatcatacga cgcctgtctc caccaacaga tcgcgcatga
      241 cactgccgag cgcgtcccga cgggcgtagt gctggtcgat atcgtcgacc ggctgccgga
      301 tccacttgcc tccggagaag agcttgtccc cgaactggcg gggtatcacg tgcaggtgga
      361 agtgaccgat gctctggctg acgttggtgt tgtagccgct gaacgagccg tccgcgccga
      421 aggcgacctc caccgcccgg ttgaggatcg ctgcccgctg gaagagcggg ccgatgaggt
      481 cagctggcag gtgttgcatg gtggcgtagt gcccctttgg gatcagcagc gcgtgcccgg
      541 gtttcagcgg gcggtgatcc agtggtccga gcgcgatgtc gtcctcccag aaaacgtttc
      601 cgcgatccac attcccgctg gtgatgcggc agaaggtgca ggttggatcg tatggtagcg
      661 gcatgttggc agttcccggg cagtgagcgt gtagagccgg atacttcccc gatcgtgctg
      721 ttccgggtca gcacaggctt tggtgtccac ctggtcggcc ggagggcgac acacggtccg
      781 gtggtcgcga agcgcccgta ctgggtttga agcagggccg acatagagcc ggcctgtacg
      841 gacgggctcc atgtccgccc ctgctacggg cgccaccacg tgcgtcaggc cccgatcagc
      901 agcgacacga cctcgaacgg gccgatctcg aacgcggccg tgttgccatt gagtgtaacc
      961 tcgcgctctt tctgcggctc ttccagaatc gtgacccgcg tcaccgatgc cggcgtctca
     1021 gtgaaggtga gttgcgcctg gccgcgagcg ccatgaggct cgtagacgcg cagcacgatc
     1081 ccgtcctggt catgcgcccg cttcagcgcg ccgagaccga gctcgatccc ggtgctggca
     1141 acgaacgcct cgcggcccgt cgcgtcgctc gccgcggcag tgaccacaag cggcgcgttg
     1201 agcgctctcg cctcgcgggt gaccccaccg tcgacccagt ccccgatgtg cggaaagagg
     1261 ctgtaggtga agcgatgctc gccctcatct gcgaacggat ccgggtgcag cggtccgcgc
     1321 acgaggctga cgcctagcgt gctgccgtgc gcgctgtggc cgtacttggc gtcgttgagc
     1381 agcgcgacac cgtacgctgg ctcggagaga tcgacgaagc ggtgggcgcc ggcctcgaat
     1441 cgtgctcgtt cccagctcgt gttctggtgt gtggcacggc gctggacacc gaacatcgtc
     1501 tcgaaggttg cctcatggct ctgcacgccc gtcgggaaga gcgcgcggat gagcatgagc
     1561 cgctcgtgcc agtcgatctg cgtgtcgatg tcgatccggc tcgatccgct cagcagtcgg
     1621 taggtctgca cgaagcggga gctgcgccag gagcgcgtga ctcgcacggc ggcccggtgc
     1681 ggtccctcct cgatcatctc catgctgtcg acatcggtga tctccatacc ggcgttctcg
     1741 taggtctcgt cgatgtccca ggcatcccat gcgcggggcc ggtcgacata ggcccagagc
     1801 tggttgcccc gatccgagag cgcttcgcgg ttgacgcgcc tgtcgaagac acggtgtagc
     1861 gtgccgtcgg ccccgacctc gaccgtgagc tcgccattct tgagcgtgac cgcgccgctg
     1921 tcccgcaatg tcgccgagac gccgccagtc ttgctcgcgt cctctgcggc gctggagacg
     1981 atgatcgatg cgaaaccgcc gagggtggtc gccggggcgt ggaccagcac tccgtcttcc
     2041 acgcgttgtg acggcaggtt gccggcgtcg gacccctccg gaagcaacgc cgtgagtggc
     2101 cgggcgttga ggtcggggtt ggtgatcgcg aacgtgtcca tgcctgagcc ggtacgcgcg
     2161 gcaatcgcct cgtcgcggac tgtgatcgcc gtcgcgatca cctcgagaag ctggggatgg
     2221 gtgtcccggt agacctccct gatggaggag ccgggcagga tgtcatggaa ttgattgtag
     2281 agcaggtcct gccaggccgc gtcgagctcc gctttcgggt agtcggcgcc gtcgagcgat
     2341 gccaatgtgg cgaatgcctc cgcctcgacc agccggtgct cgccttggcg gttgagctgc
     2401 ttcgccagcg cctgtgtcgt cagcgtggcg cggtggagct cgaggtacat ctcgccgacc
     2461 caggtcggca ggccctcacg cggcagcgaa tcgtagaact gctcgaccat gcccatctcg
     2521 agctttggca gtacgggata gtccttgatg cgatcgtagt tctggagcat gtcttcgctc
     2581 gggccgccgc cgccgtcgcc ccagccgaag gccagcaggg actcgtcgtg gatgcgtttg
     2641 ccgatgaagt tctgccaggt ctggtaggta tccagcggat tgatgtgtcc gttgtagccc
     2701 ccgttcggat tgaggaatgt gtgggcgagg acgcggctgc catcgaggcc ctgccaccag
     2761 aagaggtcgt agggaaacgc gttgttctcg ttccagatga gcttggtcgt gaagaacccg
     2821 gtgatcgctg cctgctgcag cagctgggga aggccgccgg agaagccgaa gacgtccggc
     2881 agccaggcaa ccgtgttgcg ctggccgaag gtctgctcga aaaagcgctg tccgtagaag
     2941 agctggcggg cgaatgcttc accaccagtg acctggcagt cgggctcggc ccaggagccg
     3001 ccgatcggtt cccaccggcc ttcggcaaca cgggttttga tccgctctag cagctcggga
     3061 tcgtcctcct ccatccattt gtacgcctgt gccgacgact ggttgaaggt gaagtccggg
     3121 tatttgtcca tcaggcggag cacgctcgac cacgtccggc ggaccttgcg gcgcgtttcg
     3181 gcaaccggcc acagccacgc gaggtcgatg tgtgcatgcc cgctgagcag gagtttgccg
     3241 atgggtgggt agcgctttcg gatgcgctcg agaccggagg caatcaccgc gcacgctgtg
     3301 tcacaggcag cgagtgcatc ctggggaagc g
//
LOCUS       DASYWL010000280         6818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_483796, whole genome shotgun sequence.
ACCESSION   DASYWL010000280 DASYWL010000000
VERSION     DASYWL010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6818
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483796"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /gene="hemG"
                     /locus_tag="VGR22_09935"
     CDS             <1..1120
                     /gene="hemG"
                     /locus_tag="VGR22_09935"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEV2128926.1"
                     /translation="PTRLAPIARSGLISPVGRLRMLGDYVRRPRKDEDDETLARFMTR
                     RLGGEVYSNLIEPLMAGIYAGDGSVLSMEAAFPQLRTAEREHGGLIRGVLAQRAKAQK
                     ANGATPKPGFVSFETGLHELVDALGDDIRARGGEIRTGVAVSLLERSSRGFYRVTIDR
                     DDGPETVRADGVVVATQAWAAAPLLESVDRHATQALEQIPHVSSAIVALGFDDPSVAP
                     LLNGYGYVVPRTERRPVMAMTWISSKWPNRAPEGKALIRAFVGRAGQEEVLECPDDRL
                     VEIVRDELREVLGITAAPELVQVIRWDGGMPQYTMGHLERVATLETVTGEHSGMALAG
                     NMFRGVGLPDCIASGERAADKVAADLAARGASDIAAKV"
     gene            1337..2395
                     /locus_tag="VGR22_09940"
     CDS             1337..2395
                     /locus_tag="VGR22_09940"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEV2128927.1"
                     /translation="MRQAPRSAQLLPRFLIGALALMTVLVVAAPQMGAAAPGVAWASL
                     DQRVSQLAPEHNLLVAEFTGNACRPIHSVNAGDQLAIASTFKIYVLGELARQVQAGEI
                     SWDERITLTDQLRSMPSGDYAWVPAGQQVSVRDLAQAMIWNSDNTATDHLIDRLGREE
                     VERTFAAYGHANPVANQPLLLTRELFAIKMWQPAEWMARFTAAADEEQLQLLTSEIDG
                     IAINPSGGWGYWNGPTAIDGIEWFASAEDLCRAMVGLWTMGAQPGLEPVREILSGNRG
                     GVGDTAVWPRVGFKAGYEAGVVNSTYLLERSDGRVFFVSVGFNHPQWVVDQNAPGALL
                     GPVFSCLAAYQQSGDCDT"
     gene            2759..3223
                     /locus_tag="VGR22_09945"
     CDS             2759..3223
                     /locus_tag="VGR22_09945"
                     /inference="COORDINATES: protein motif:HMM:NF037033.4"
                     /note="Related to clavulanate biosynthesis protein Cpe,
                     which has an isomerase-like N-terminal domain and a
                     beta-lactamase-like C-terminal domain; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cpe/LpqF family protein"
                     /protein_id="HEV2128928.1"
                     /translation="MLIRSLLTMLALPLFLLAPCAAQTPEVTPDSGDAVADHVDWAIS
                     LFDGGADDLAVEDVEVRFDSNFLRLVPPEEFTTTIRPLAETLGPLELVEKRATEENEH
                     VAVYRAESGDVVMIGLAVDPVTGLMAGFFVIPTQLAGSDATPAASPAASPIV"
     gene            3296..4456
                     /locus_tag="VGR22_09950"
     CDS             3296..4456
                     /locus_tag="VGR22_09950"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEV2128929.1"
                     /translation="MQVSVLRSFFVMFLVAALTPPVTAAETAQATVALEAWMVNAFEQ
                     PTYVTHAGDERLFVTEQAGTVQVVPDVADPSTGAQLFLDITDRVGSSGSEQGLLSMAF
                     PPESHAGSPVYVSYTALDGGTVVSRLDVAEDGLSADPDSERVILTHPQPYPNHNGGLI
                     LFGPDDLLYIGLGDGGSGGDPEGNAQNPDTWLGSILRIDVNPDVVGDAGYAVPAENPF
                     VGDADAQPEVWMYGLRNPWRFAFDAAGTMIAVADVGQNAVEEATILPLEAARGANLGW
                     PILEGTFCYEVADCDSTGTILPSLEYTHEEGGCSVTGGEFIEAGYLYADYCSGLLWLA
                     TQTGDGWEAGMPLETRLSISSFGRGSDGAVYVVDRAGGSIYHVVVPSAHFGA"
     gene            4530..5564
                     /locus_tag="VGR22_09955"
     CDS             4530..5564
                     /locus_tag="VGR22_09955"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2128930.1"
                     /translation="MTSRRFTSHRRSFLTSSVGIATVAAVGPPVRALGQQATPAATPV
                     AVEPPDMTGATYEFQLGSFTCWSVSDGANAGPFVKLIVFGATPDEEEEAIIRETGIDL
                     SQLVSQHTSTVIDTGSELVLVDTGAGAPGLPETGILADNLRRMGIQPGEIDMVVLTHG
                     HPDHIGGTVTATGNPLYSNARYVMAQEEWEFWTDPVRVEEEAVPADFLETFLATVDRA
                     LLPLEGAIELVGYGEELLPGITTVDARGHTPGHMGLMVESEGEQLWVMGDAALNPIDL
                     PYPEVIGAPDINPEQVIETRRRLFAEIAGSGNMAVFNHFHPFPSLGLIESEGDAWAWL
                     PIEVEDDAEG"
     gene            complement(5779..6228)
                     /locus_tag="VGR22_09960"
     CDS             complement(5779..6228)
                     /locus_tag="VGR22_09960"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEV2128931.1"
                     /translation="MDAVLRALAIYVILMLIFRIAGKRALAQITTFDFVLLLVIGEAT
                     QQALLGNDYSIANAALVITTLIGIDVGLSLLQQRFPAIGPLTEDVPLVLVNEGEVLRE
                     RLDKSRISESEILEAARDKQGLERMDQIKFAVLERAGTISIIPKQQG"
     gene            complement(6267..6614)
                     /locus_tag="VGR22_09965"
     CDS             complement(6267..6614)
                     /locus_tag="VGR22_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128932.1"
                     /translation="MGDGALVLEYERFVRHGARTTFSIAADSSQVENGEIELWMSSEY
                     FDAIDLQNISPQPNEVRDAGDRRIMVFLVEDPSTTLTVELSARPREIIGWISAEAGVV
                     DGPSIAFDQLSYP"
BASE COUNT         1147 a         2107 c         2346 g         1218 t
ORIGIN      
        1 cccgacccgt ctcgcgccga tcgcgagatc agggctgatc tcccccgtcg gtaggctgcg
       61 aatgctcggc gactacgtgc gtcggccgcg caaggacgag gacgacgaga cgctcgcgag
      121 attcatgacc cggcggctcg gtggcgaggt ctacagcaac ctcatcgagc ccctgatggc
      181 ggggatctac gcgggtgacg gaagcgtgct cagcatggag gcggccttcc cccaactgcg
      241 tacggcagag cgcgagcacg gcgggctgat caggggcgta ctcgcccagc gggcgaaggc
      301 gcagaaggcg aacggcgcca cgccgaagcc ggggttcgtc agcttcgaga ctggcctcca
      361 cgagctcgtc gacgcgctcg gcgacgatat ccgggcgcga ggcggcgaga tccgcaccgg
      421 ggtggcggtg tcgctgctgg agcgatccag ccggggtttc taccgcgtga cgatcgaccg
      481 cgacgatggc ccggaaacgg tacgcgccga cggtgtggtc gtcgcaacgc aggcatgggc
      541 ggccgccccg ctgctcgaga gcgttgaccg gcacgccacg caggcgctgg agcagatccc
      601 ccacgtctcc agcgcgatcg ttgcgctcgg gttcgacgat ccgtcggtgg cacctttgct
      661 caacggatac ggctatgtgg tgccgcgcac ggagaggcgg ccggtgatgg cgatgacgtg
      721 gatctcgtcg aagtggccga accgggctcc tgagggcaag gcgctcattc gcgccttcgt
      781 cggccgcgcc gggcaggaag aggtgctcga gtgcccggac gaccgactgg tcgagatcgt
      841 tcgcgacgag ctgcgcgagg tgctggggat caccgccgcg ccggagctgg tccaggtgat
      901 ccggtgggat ggcgggatgc cgcagtacac gatggggcac ctggagcggg tggcgacgtt
      961 ggagaccgtc acgggcgagc actcggggat ggcgctggcc ggaaacatgt tccggggcgt
     1021 cgggttgccg gactgcatcg cctccgggga gcgcgcggcg gacaaggtcg cggctgatct
     1081 tgccgcccgt ggtgcgagcg atatcgccgc gaaagtatag ggttgcctgc ttgacatgtg
     1141 atgtcagatg gggacgccgt acccgggcgg tgtgtgtcaa cgctgccggg atggccatat
     1201 agcgatagcg gctgacaatc tggcacatct tgttgcccaa cgatcctcgg gctagcatgg
     1261 agtgtacgga cgcgctgtac agacagtggc cgaacgctga cgatcgtcgt tgccatgccg
     1321 ggggagactc caccagatgc gacaagcacc tcgatccgcc cagctgctgc cgcgcttcct
     1381 gatcggtgcc ctcgcgctca tgaccgtgct cgtcgtcgct gccccgcaga tgggcgccgc
     1441 cgcgccaggg gtggcctggg cgagcctcga ccagcgggtc agccagctgg cgcccgagca
     1501 caacctcctc gtcgccgagt tcaccggaaa tgcctgccga cccatccaca gcgtcaacgc
     1561 cggtgaccag ctcgccatcg cgtcgacctt caagatctat gttctgggcg agctcgcccg
     1621 gcaggtgcag gccggcgaga tcagctggga cgaacgcatc acgctgaccg accagttgcg
     1681 ctcgatgcct tccggggact acgcctgggt tcccgccggg cagcaggtca gcgtgcggga
     1741 tctggctcag gccatgatat ggaacagcga caatactgcc acggaccatc tgatcgaccg
     1801 gctcggtcgc gaggaggtcg agcgcacctt cgccgcctat ggccacgcca accctgtcgc
     1861 caaccagccg ctgctgctca cgcgcgagct cttcgcgatc aagatgtggc agccggccga
     1921 gtggatggcg cggtttaccg ccgccgccga tgaggagcaa ctccagctcc tgacgagtga
     1981 gatcgatggg atcgccatca atccctctgg cggctggggg tactggaacg ggccgacagc
     2041 gatcgatggc atcgagtggt tcgcctccgc cgaggacctc tgccgggcca tggtgggtct
     2101 gtggaccatg ggggcccagc cagggctgga gccggtgcga gagattctga gtgggaatcg
     2161 cggcggggtg ggggacaccg ctgtctggcc gagggtcggc ttcaaggccg gctacgaggc
     2221 cggggtggtg aactccacct acctgctgga gcgcagcgac ggacgggtct ttttcgtctc
     2281 ggtcgggttc aatcacccgc agtgggtggt cgaccagaac gcgcccggtg cgttgctggg
     2341 gccggtgttc agctgccttg ccgcctacca gcagtccggc gactgcgaca cctgacaagc
     2401 cagtcttccc atcgatgtca tgttgtcagc cttgtccgga tcgggatacg gtcggaggtt
     2461 acagtagtct cctctgcagg cttcgctggc gagtggagca gtctcttcgg caatcattac
     2521 ttaacgccag gcaggcaaga gatttctcga cagcaggccc gctcacgctc gccttgcgct
     2581 cggaatgaaa ggttcggtgc aggcttcggt ttcaccatgc tggcagcttc cgacggtttg
     2641 cgtcttcggc gggagtcgcc gtacctccta aagattcgtg acgtgagctc actatttggt
     2701 gagagcaccg tgcgagggag gataggagcg atgctcgcga aaggttcctg tgctcaccat
     2761 gctgattcgc tccctgctga ccatgctcgc gcttccactg ttcctcttag caccatgcgc
     2821 agcacagacg cccgaggtga caccagactc cggtgatgcg gtcgccgatc atgtcgactg
     2881 ggcgatctcg ctcttcgatg ggggtgccga cgaccttgcc gtggaggatg tcgaagtccg
     2941 cttcgacagc aacttcctgc gcctcgtgcc gccggaggag ttcactacga cgatacggcc
     3001 gcttgccgaa actctcgggc cgctggagct ggtcgagaag cgggccactg aggagaacga
     3061 gcatgtcgcg gtctaccggg ccgagtcggg cgacgtcgtg atgattggtc tcgcggtcga
     3121 tccggtgacg gggctcatgg ccgggttctt cgtcatcccg acgcagttgg ccggcagcga
     3181 cgcgacacct gccgccagtc cggctgcctc gcccatagtg taaggacaag gggcggtgat
     3241 ggactttcaa ttcaatacgg cttcattgtt cgggaaggat gattgagagg tcctggtgca
     3301 ggtttcggtg ctccgctcgt ttttcgtgat gtttctggtc gctgccctca cgccaccggt
     3361 gactgccgcc gagacggcgc aggcaacggt ggcgctcgag gcgtggatgg tcaatgcctt
     3421 cgagcagccg acctacgtaa cgcatgccgg cgacgagcgg ctcttcgtca cggagcaggc
     3481 gggcaccgtg caggtggtgc cggatgtggc cgacccctcc accggcgcac aactctttct
     3541 cgatatcacc gatcgtgttg gctcctccgg cagtgagcag gggctgctga gcatggcctt
     3601 cccgcccgag tcgcatgcgg ggagccccgt ctacgtctcc tacaccgctc tggacggggg
     3661 gacggtggtc agccggttgg atgtggcgga ggacggcctc agtgccgatc cggacagcga
     3721 gcgagtgatc ctcacgcatc cccaaccgta tccgaaccac aacggcggac tgattctttt
     3781 cggtccggac gacctgctct atattgggtt gggcgatggc ggctcggggg gtgacccgga
     3841 gggcaatgcg cagaatcccg acacgtggct cgggagcatc ctgcggatcg acgtgaaccc
     3901 ggacgtagtc ggtgacgcgg ggtacgccgt tccggcagag aatcccttcg tcggcgatgc
     3961 ggatgcccag ccggaagtct ggatgtacgg cctgcgcaac ccctggcgct tcgccttcga
     4021 tgcggcgggc acgatgatcg ccgtggccga cgtcgggcag aacgcggtcg aggaggccac
     4081 gattctcccg ctggaggcgg cgcgcggtgc caatctcggc tggccgatcc ttgaaggcac
     4141 cttctgctat gaggtggcgg actgcgattc tactggcacg atactgccgt cgctggaata
     4201 cacccatgag gagggcggct gctcggtgac cggcggggag ttcatcgaag caggctacct
     4261 ctatgccgac tactgctccg gcctgctctg gctggctacg cagacggggg atggctggga
     4321 ggcgggcatg ccgctggaga cccggctctc gatctcgtcc ttcgggcggg ggagtgacgg
     4381 tgctgtctac gttgtcgatc gggccggcgg gtcgatttac cacgtcgtgg tcccgtcggc
     4441 gcattttggg gcatagccct tgcggcgcgt gctgctgagg ctcatggtga gtttctttct
     4501 catcaaggcg tgcaacagga ggtgcgtcca tgacatcacg gcgtttcacc agccatcgac
     4561 ggagcttcct caccagttcg gtcggcatcg cgactgtggc ggccgtcggc ccgccggttc
     4621 gagcgctcgg ccagcaggcg acgccggccg ccacaccggt cgccgtcgag ccgccggaca
     4681 tgaccggagc cacctacgag ttccagttgg gctcgttcac ctgctggtca gtcagtgacg
     4741 gcgcgaatgc cggtccgttc gtcaagctca ttgtcttcgg cgccactcct gacgaagagg
     4801 aggaggcgat catccgggag acgggcattg acctgagcca actcgtctcg cagcacacct
     4861 cgaccgtgat cgacaccggg tccgagctgg tgctggttga caccggggcc ggagcgcccg
     4921 ggctgccgga aaccggtatc ttggctgaca acctgcgccg tatgggcatc cagccgggtg
     4981 aaatcgatat ggtggtgctg acccacggtc accccgacca catcggaggt accgtgaccg
     5041 ccactggcaa cccgctctac agcaacgcgc gctatgtgat ggcgcaggag gagtgggagt
     5101 tctggaccga tccggtccga gtcgaagagg aggcggtacc ggccgacttc ctggaaacct
     5161 tcctggcgac agtcgaccgt gccctgctgc cactggaggg ggccatcgag ttggtgggct
     5221 acggcgagga gctcctgccg gggatcacca cagtcgacgc gcgtgggcac acgccggggc
     5281 acatgggcct gatggtcgaa tccgagggtg agcagctctg ggtgatgggg gatgctgcgc
     5341 tgaacccgat cgatctgcca tatccagagg tcatcggcgc gccagacatc aacccggagc
     5401 aggtgatcga gacacgccga cggttgttcg cggaaatcgc cggcagcggc aacatggcgg
     5461 tgttcaacca ctttcaccca ttcccgagtc tcggcctgat cgagagcgag ggcgatgcct
     5521 gggcttggct accgatcgaa gtggaagacg acgcggaggg gtagccggga ccagttgatg
     5581 ggtggtaccg aacccgggtg tgtatgcttc accttcccgt cggcgcagcc ctggggcggg
     5641 tgtcatcctg ggagcggtca ggcggacatg aggtgcgccc atacgagtgg acgggttcac
     5701 ctactgttca cccggattcc gcatggatct ccccggtcgc tggcaagcgg ccggggagat
     5761 cttgccgtga tgaaacggct atccctgctg cttgggaatg atcgagatcg ttccggcacg
     5821 ctccagcacc gcaaacttga tctgatccat ccgttcgagt ccctgtttgt cccgtgcagc
     5881 ttcgaggatc tccgactcgg aaatgcggct cttgtccagc cgttccctaa gcacctcacc
     5941 ctcattgacg agcaccagtg ggacgtcctc cgtcaacggc ccgatcgccg ggaagcgctg
     6001 ctgcagcagc gagaggccga cgtcgatgcc gatcagcgtc gtgatcacca gcgcggcgtt
     6061 ggcgatcgag tagtcgttgc ccagcagggc ctgctgggtc gcctcgccga tgaccagcag
     6121 cagcacgaag tcgaaggtcg tgatctgggc gagggcgcgc ttgccggcga tccggaagat
     6181 cagcatcagg atgacgtaga tggcaagcgc tcggaggacg gcgtccatgg gatctctcct
     6241 ccagtggtgt cagggcaggc gtgcgcctag gggtagctga gttggtcgaa agcgatggag
     6301 ggcccgtcga caacgccggc ctcggccgag atccacccga tgatctcccg gggacgcgcg
     6361 gagagttcga cggtgagcgt tgtggaggga tcctccacca gaaaaaccat gatgcgacgg
     6421 tcaccggcat cacggacttc attgggctgc ggggagatgt tctgcaggtc gattgcgtcg
     6481 aaatactccg acgacatcca cagctcgatc tcgccgttct cgacctggct gctgtcggca
     6541 gcgattgaga aggtcgtgcg cgcaccgtgg cggacgaagc gctcgtactc aagcaccagg
     6601 gccccgtcgc ccaccccacg gcgctcagga gccaggtgct gaagaacccg accagcgcga
     6661 gcacgatgag aaggagcatc agggcccagc cgatccgctg caccttccac tggcgctcct
     6721 ggaagggaat gttctcgctg acctccagcg cgccgacgcg gtggggttcc gacatcaaaa
     6781 gctcctcccg gtgatcgcct tgccaagctg ccagatgg
//
LOCUS       DASYWL010000281         2563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_295721, whole genome shotgun sequence.
ACCESSION   DASYWL010000281 DASYWL010000000
VERSION     DASYWL010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295721"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..619)
                     /locus_tag="VGR22_09970"
     CDS             complement(191..619)
                     /locus_tag="VGR22_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128933.1"
                     /translation="MSDRRRESSEGQAQGKEGRSPAEWTTLAISIGIIAGFLGVVTWL
                     YFRGSEEPPLITVEVQMESLREEDGAWYLPIEVRNDGDRTVENTQVEGTLRTGEGEPQ
                     EASITVAFLAGGERVEGTLVFSDDPRNGELEVGPTSYQIP"
     gene            complement(616..1458)
                     /locus_tag="VGR22_09975"
     CDS             complement(616..1458)
                     /locus_tag="VGR22_09975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02587 family membrane protein"
                     /protein_id="HEV2128934.1"
                     /translation="MATAGGQTWGQEARSFARAFAGAYIFAVPLLFTMEMWWIGQYAT
                     RWKLLAFLLVALLANFGLTYVAGFKRESSLFATIGQTLDAVAVGIVAATVMLLVLNRI
                     GPNQPIDSTIGMILVQAVPLSIGASVANEVFGGRRDNGRQGHADPPDLPSWQELASDV
                     GATAIGGVFVGFSIAPTEEVPMLAAGLEYPHLLALIGFTLLLSYGIVFASGFDRSRAQ
                     GLFQSPITETALAYVASLVVAFIALYLLDQIDIGQPLLSIVEQVLVLGVPTTVGGAAG
                     RLVV"
     gene            complement(1580..2068)
                     /locus_tag="VGR22_09980"
     CDS             complement(1580..2068)
                     /locus_tag="VGR22_09980"
                     /inference="COORDINATES: protein motif:HMM:NF016874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HEV2128935.1"
                     /translation="MRTIYLASPLGFAESTRPFMDHLTARLRQHVVVNNPWDIPESVD
                     PFSDALSIDSVRERDHRLAAINRELGRRNREAIDASDGLFAVLDGVDVDSGTAAEIGY
                     AFAKGKYICGLRTDLRLAGDNPGSIVNLQVEYFIFESGGRIVTTAEELVELVEEFAAN
                     GT"
     gene            complement(2088..2483)
                     /locus_tag="VGR22_09985"
     CDS             complement(2088..2483)
                     /locus_tag="VGR22_09985"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEV2128936.1"
                     /translation="MTAVYDIIFDGGSKGNPGLGYGSYQITRDRAIVAHTQLDYGDNV
                     TNNQAEYWTLLNALRWLADDLGDEARDASVTVHGDSQLVIKQLKGEWKIKDAKLRGIA
                     METRAQMDRFKDVSLAWHRRDVSVQQLGH"
BASE COUNT          441 a          838 c          838 g          446 t
ORIGIN      
        1 acccgatcct gctcggcccg accggcgtac cgtcgccgct acatggacga ggggggcagc
       61 cggagcctat ccccgccctt cggccgttgc acgcgttctt ccacttcccg catctgacga
      121 ccttgctgtc gaagccgcta ttcgagctcg ctggttgaca ccaaggaggc agggccgtta
      181 ggtagggcca ttatgggatc tggtagctcg tcggccccac ctcgagctcg ccgtttcgag
      241 ggtcgtcgct gaagacgagg gtcccctcca cccgctcccc gccggcgagg aacgctaccg
      301 tgatggacgc ctcctgaggc tccccctcgc cggtgcggag cgttccctcg acctgggtgt
      361 tctcgacggt gcggtcaccg tcgttgcgta cctcgatcgg caggtaccat gccccatcct
      421 cctcgcgcag gctctccatc tgcacctcga cggtgatgag cggcggctcc tcgctgccgc
      481 ggaaatagag ccaggtgacg accccgagaa aaccagcgat gataccgata ctgattgcga
      541 gagtcgtcca ctcagccggt gagcgcccct cctttccctg cgcctggccc tccgatgatt
      601 cgcgcctgcg gtcactcata ccaccaatct tcctgctgcg ccgccgaccg tggtgggcac
      661 gcccagtacg aggacctgct cgacgatgga gagcagcggt tgcccgatat cgatctggtc
      721 aaggagatag agcgcgatga aggcaacgac gagcgacgca acgtaggcca gtgccgtctc
      781 ggtgatcggt gattggaaaa gaccctgcgc ccgagaccgg tcgaagccgc tggcgaagac
      841 gatgccgtag ctgagcagga gcgtgaagcc gatgagcgcc agcaggtgcg gatactccag
      901 cccggcggcc agcatcggca cttcctcggt cggcgcgatc gagaaaccga cgaagacgcc
      961 gccaatggcg gtcgcgccga catccgacgc gagctcctgc caggaaggga ggtcgggtgg
     1021 atccgcgtgg ccctgccggc cattgtctcg cctgccgccg aaaacctcgt tggcgacgga
     1081 tgcgccgatg ctgagcggca ccgcctggac gagaatcatg ccgatagtgc tgtcgatcgg
     1141 ttggttcgga ccgatgcggt tcagcacgag cagcatcacc gtggcggcga ctataccgac
     1201 cgccacggca tcgagcgtct gcccgatggt cgcgaacagc gagctctcgc gcttgaagcc
     1261 ggcgacgtag gtcaatccga agtttgccag gagcgccacg agaaggaacg cgagcagctt
     1321 ccatcgggtc gcatattgcc cgatccacca catctccatg gtgaacagga gcgggacggc
     1381 gaagatgtag gcgccagcga acgcgcgcgc gaagctgcgc gcctcctgac cccatgtctg
     1441 ccctccagct gtcgccacgt tgccactccc tctcgcgcca ggatggtgcg gtgcgaagtc
     1501 gatgaacgtg ctgcacgttc cctgccgcgc ggcaaaccga gccggcggcg agcacggcgt
     1561 cgccgggagc ggaaagccgt caggtgccgt tcgcggcgaa ctcctcgacg agctcgacga
     1621 gctcctccgc cgtcgtcaca atgcggccgc cgctctcgaa gatgaagtac tcgacctgca
     1681 gattgacgat cgatccgggg ttgtctccgg caaggcgcaa gtcggtccga agcccgcaga
     1741 tgtacttgcc cttcgcgaac gcatagccga tctcggcggc ggtcccggag tcgacgtcga
     1801 cgccgtccag caccgcgaag aggccgtcgc tggcgtcgat cgcctcgcgg ttcctgcggc
     1861 caagctcacg gttgatcgct gcgagccggt gatcccgctc ccgcaccgag tcgatggaca
     1921 gcgcgtcgct gaacgggtca acgctctccg ggatatccca gggattgttg acgacgacat
     1981 gctgacggag ccgagcggtg aggtggtcca tgaacgggcg ggtggactcg gcgaaaccaa
     2041 ggggggaggc gaggtagatc gttcgcatcg aatgtctgcc tccgggctca atggccgagt
     2101 tgttggacag agacatcacg ccggtgccat gcgagcgaca catccttgaa gcggtccatc
     2161 tgcgccctcg tctccatcgc gatgccgcgc agtttggcgt ccttgatctt ccactcgccc
     2221 ttgagctgct tgatgacgag ctggctgtcg ccgtgcaccg taacagacgc gtcgcgggcc
     2281 tcgtcaccga ggtcgtcggc gagccagcgc agcgcgttga gcagcgtcca gtattccgcc
     2341 tggttgttgg tgacgttgtc tccgtagtcg agctgagtgt gcgcgacgat cgcgcggtcg
     2401 cgggtgatct ggtagctacc gtagccgagg cccgggttcc ccttggagcc accgtcgaag
     2461 atgatgtcgt agactgcggt cacatgtcca tccttgcgaa atggcggccg atcattccgc
     2521 aaggatggta cacggtctcg ggggtgcccg gagcgggcgg ggc
//
LOCUS       DASYWL010000282         7172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_277491, whole genome shotgun sequence.
ACCESSION   DASYWL010000282 DASYWL010000000
VERSION     DASYWL010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7172
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277491"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="VGR22_09990"
     CDS             complement(<1..846)
                     /locus_tag="VGR22_09990"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEV2128937.1"
                     /translation="MHSPRRPRVFACAVLLAVWLGISGLLAPSATLADTDLVIGGEAV
                     VAYAQGDNVRVRDDATYDAGIITSVPEGTTVSVLDGPFSDGDGNTWYYVSAVGSTGYM
                     VNDYLTNAGGGAPSGGTAGGTVTATDNVHLRSGPSVGYASIGKIPTGTSVAVLDSMDD
                     WLRVSWNGFEGWSYGEFFIDGGSAAPAPTEDPAPAPEPEPEQEPEITWIDAGTRYTND
                     SVNFRSGPSLDSSVYELLPAGIAVELTGKSQNGFAEGVANGYTGWVSLDFLTATAPED
                     PEPDVE"
     gene            complement(1197..2441)
                     /gene="glp"
                     /locus_tag="VGR22_09995"
     CDS             complement(1197..2441)
                     /gene="glp"
                     /locus_tag="VGR22_09995"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEV2128938.1"
                     /translation="MSARSSDPITLSVDEARERILAEFAPLELTAAPLDDALGLVTTE
                     PVVALGDLPPFDNSAMDGYALRSADTAAASDATAVTLQVIGQAPAGYRAPTAVPPGAA
                     LRIMTGAPIPDGADAVVRFEHVAMLAADHIAIHRPVQQGENIRPAGEDARWGEVILTV
                     GTRIRANEIALLASSGHASVAVHRRPRVGVLVTGDEVIAPGEPLGPGQIWNSNGPMVA
                     AQVRQSGGVPVVLGVTTDSPAAVRGRLAEIDSLDLLLTTGGISVGDFDVVKDVLREEG
                     HIELWSLRIKPGKPLAFGQVGQTPVIGLPGNPVAAAVAFWQFGYPAVRAMLGHREVQL
                     PQIEARLLDRAENRGDRRQFVRVRVEQDAQGYTARFAGGQGSAMLTSLAASNGLLVVP
                     EACDVAGPGDLMTVQMPGWDLG"
     gene            complement(2709..3287)
                     /locus_tag="VGR22_10000"
     CDS             complement(2709..3287)
                     /locus_tag="VGR22_10000"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEV2128939.1"
                     /translation="MLIGGRSRRMGGPKALLRLAPGEPTLLELVAGAMSEVASDVTLV
                     GRASWPIPESLAGLHRVRDEGNGAADGVVAALRASRFDECLVVGCDMPFLNVALLREM
                     GTLSKSEGRGVIVSESAGLHPLHAVYRRKNLARIESVVATGERSLVAIVQALGMAAID
                     LDVPERDSAVRWSVFNVNTPEDLETARAHAGV"
     gene            complement(3314..4906)
                     /gene="metG"
                     /locus_tag="VGR22_10005"
     CDS             complement(3314..4906)
                     /gene="metG"
                     /locus_tag="VGR22_10005"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF020892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEV2128940.1"
                     /translation="MTDASDKRFYITTSIPYVNAMPHIGFALEAIQCDVLARYHRQNG
                     DDTRYLSGTDENALTNVQAAEREGVSVRELVDRNAPRFAALQEPLRLSWDDFIRTSTD
                     PRHEPGAQKLWRTMANNDDIYSQHYAGLYCVRCERFYKPEELIDEKCPIHHIYPDRVD
                     EENYFFRLSVYENRLRDLITSNEMLVQPEHRREEMLSFINQGLEDFSISRQAGRGRGW
                     GVPVPDDPNQVMYVWVDALSNYITALNYAEDGALFDRYWLNNPNRVHVVGKDIIRFHV
                     IYWPAMLMSAGLPVPSKVNVHEFLTHEGEKMSKSRGNVVDPIVLVNQYGADAVRWWML
                     RDMPRTSDGDFSYQRLEDRYNMDLANDLGNLINRSVGMLHRYRGGVVPSALAAAEADG
                     VLHNIADTIPARIERALDRFDFREALAPLQELVTAANRYVDDRKPWELNKAAKNGDEE
                     AARQIDTVLANLIESLRVLAVHLRPFLPDGADRISGQLNLGSEEAKGDTASPEWGDAL
                     AGVTLPKGAPVFPRIEREEDVA"
     gene            complement(5440..6567)
                     /locus_tag="VGR22_10010"
     CDS             complement(5440..6567)
                     /locus_tag="VGR22_10010"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2128941.1"
                     /translation="MNSPAQRSLRIGILGSGNIARHHARGWSTYPDRAEIVGFADIAT
                     ARAQQMSNTYTGGSAIAFTSGNEMLAEADLDVVDICLPHHLHTDAIIAAAEAGKAILC
                     EKPLCTTLDDATRIRDVIQKTRVTFMGAHNQLFQPSLLEARRLMAGGFLGTPYLLRSI
                     ETFQARKWEPFGKAETDQRDWGWRSDIAQAGGGELIDTGYHGTYRLLTLADSRPVEVT
                     AILSKFFQKGLATEDTAMVIVRFESGAVGEILTSWALDVVGNKHFEVQGELGALSGGP
                     GSLEHQLYGWADSATKTFEPVHSFTLEIEHFIEVVTGEAANPADIDETARTLQVIKAA
                     YLSADLGRTVQLPEDPTAPATADGHAASTPTNVPVLDAAKA"
     gene            complement(6736..>7172)
                     /locus_tag="VGR22_10015"
     CDS             complement(6736..>7172)
                     /locus_tag="VGR22_10015"
                     /inference="COORDINATES: protein motif:HMM:NF038065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEV2128942.1"
                     /translation="AAVMYMVTLFFVYGFLVSSFDNPFDTTRHWTHTVVHQAIPVAVV
                     LDFILRPFANRLSWPTAFLWTVYPIAYLAWSLIRGEIDGWYPYDFINPGEVGGWETVG
                     LNVIGVTIGFVALGLFLVWVSHWYHRLATASPPIRPQAASPR"
BASE COUNT         1172 a         2349 c         2383 g         1268 t
ORIGIN      
        1 ctccacgtcg ggttccggat cttcaggagc agttgccgtc aggaaatcga gtgataccca
       61 gccggtgtat ccgtttgcga cgccctcagc gaagccgttc tggctcttgc cggtgagctc
      121 gaccgcgata ccggccggca gtagttcgta gacgctgctg tcgagcgacg gtcccgagcg
      181 gaagttcacc gaatcgttgg tgtatcgcgt gccggcgtcg atccaggtga tctctggctc
      241 ttgttccggt tctggttcag gcgcaggggc cgggtcctcg gtcggggcag gtgcagccga
      301 gccaccgtcg atgaagaact cgccgtagga ccagccctcg aacccattcc agctcacgcg
      361 tagccagtca tccatgctgt ccagcacggc gactgacgtg cccgtcggaa tcttgccgat
      421 gctggcgtac cccacgctcg gaccgctgcg caggtgcacg ttgtcggttg cggtcacggt
      481 gccacctgca gtcccgccgc tcggcgcacc accaccggcg ttcgtgaggt agtcattcac
      541 catgtagccg gtgcttccca cggcggagac gtagtaccac gtgttcccgt cgccgtcgct
      601 gaatggcccg tcgagcacgc tgaccgtggt gccctctggc actgaggtga tgatgccggc
      661 gtcgtaggtg gcgtcgtcgc gaacgcggac attgtccccc tgcgcgtagg cgacgaccgc
      721 ctcaccaccg atcacgaggt cggtgtcggc tagggttgcc gatggcgcca gcaggccgga
      781 gattccgagc cagacagcga ggagtacggc gcacgcgaaa acgcggggcc tgcggggcga
      841 gtgcattcct tccctccgat acaatcccgc aatcgctccc gagacgaccc ctctgccggg
      901 tggtcgccac tcaggtgcga tcggttgaac accatgccgc cgtccggatg gacgtgctgc
      961 gtaggtcgac atccttcggc gtgatggcac ccgcaggccc agccagccgg ggcgagtacc
     1021 tggccgttct tggatgtccc gctgattgtc tgtgtccgta cgcgacccga tcaacgtgta
     1081 cgaacgggat attcagcacg aacgttgacc agaggggtat acggacgcac ctcaattggg
     1141 tcaggatggg gagaacgggg agattaggcg ctgtcggcca gtcggatgtt ggccacctat
     1201 cccaggtccc agccgggcat ctggacggtc atcaggtcgc cgggtccggc aacatcgcag
     1261 gcctccggta cgaccagcaa cccgttcgac gctgccagcg acgtcagcat cgccgatccc
     1321 tgtccgccgg cgaaccgcgc ggtgtacccc tgcgcatcct gttcgacacg tacccggacg
     1381 aactgccgcc gatcgcctcg attctcggcc cgatcgagca ggcgcgcctc tatctgcggc
     1441 aactgaacct cccgatgccc cagcatcgca cgtaccgccg gatacccgaa ctgccagaag
     1501 gcgaccgccg ccgcgaccgg attcccgggc aacccgatca cgggcgtctg ccccacctgg
     1561 ccgaaggcga gcggtttgcc cggcttgatg cgcaggctcc agagttcgat gtgtccctcc
     1621 tcacggagca catcctttac cacgtcgaag tcgccgacgg agatgccgcc ggtggtcagc
     1681 agcaggtcga ggctgtcgat ctcggccagc ctcccccgga cggcggcggg agaatcggtc
     1741 gtcacgccga gtaccaccgg caccccgccc gactgccgca cctgcgcggc gaccatcggg
     1801 ccgttgctgt tccagatctg ccctggtccg agcggctcgc cgggcgcgat cacctcgtca
     1861 ccggtcacca gcaccccgac gcgaggccgg cgatgcaccg ccacgctggc gtgaccgctg
     1921 ctggccagca gcgcgatctc gttcgcccgg atgcgggtgc cgacggtgag gatcacttcg
     1981 ccccaccggg cgtcctcacc ggcgggacgg atgttctcgc cctgttgcac cggacgatgg
     2041 atcgcgatgt ggtcagcagc gagcatcgcg acatgttcga agcgaaccac ggcgtcggct
     2101 ccgtcaggga tcggcgcgcc ggtcatgatg cgcagggcgg cgcccggcgg cacggcggta
     2161 ggggcgcggt acccggccgg cgcctggccg atcacctgaa gcgtcacggc tgtggcgtcc
     2221 gatgcagctg ccgtgtcggc ggatcgaagg gcgtagccat ccatcgccga gttgtcgaac
     2281 ggcggaaggt caccaagagc gaccactggc tcggtggtca cgagcccgag cgcgtcgtcg
     2341 agtggtgctg cggtcagctc cagcggcgcg aactcggcga gaatacgctc ccgcgcctcg
     2401 tcgacactca aggtgatggg atcggacgaa cgagcggaca tggccgaggc tttctgatga
     2461 tgacaaccgg atgaactgtc gtgacaggag catagcctga ttgcctgcta acatcccgcc
     2521 atttcgaccg aagcggcgaa atctcttgcg tgatctgcat cattcccggt tcgggagaga
     2581 tttgtccact ccaccctcgc tgcgctcggt tgcgctcgat atgacggttg ggaggatcga
     2641 cgtcgaagtc agttgcgagc gggtgttggc cactggcaac cggatggact cgctccgcag
     2701 caccgtccct acacgccggc gtgggctctc gcagtctcaa ggtcctcggg cgtgttgacg
     2761 ttgaagacgg accagcgcac ggcgctgtcc cgctctggga cgtcgaggtc gatggctgcc
     2821 atgccgagcg cctggacgat ggccaccagc gatcgctcgc cagtggcaac caccgactcg
     2881 atccgcgcga gattcttccg ccggtagacg gcatggagcg ggtgcaggcc ggctgattcg
     2941 ctcacgatca cgcccctgcc ctcgctcttc gaaagcgtgc ccatctcgcg cagcagtgcg
     3001 acattcagaa acggcatgtc gcacccgacg acgagacact cgtcgaagcg cgaggcacgc
     3061 agcgcggcga ccacaccatc ggccgcaccg ttgccctcgt ctcgcaccct atgcagcccg
     3121 gcgagcgact ccggaatcgg ccacgatgct cgcccgacga gcgtgacatc actcgcgact
     3181 tcgctcatgg caccggccac gagttccagc agcgtcggct cgccgggagc gagacgcagc
     3241 agcgcctttg gcccgcccat cctccggctg cgcccgccga tcagcaccgc tgccgagaca
     3301 tccattggcg cgactatgcg acatcctcct cgcgctcgat gcgtgggaag acgggcgctc
     3361 ccttcggcaa ggtgacgccc gcgagcgcgt caccccactc cggactggcc gtgtcgccct
     3421 tcgcctcctc ggagcccaga ttgagctggc ccgagatgcg gtcggcaccg tccggcagga
     3481 agggtcgcag gtgcaccgcc agcacgcgca gcgactcgat caggttggcc agcacggtgt
     3541 cgatctgccg ggcggcctcc tcgtcaccgt ttttcgccgc cttgttgagc tcccacggct
     3601 tgcggtcgtc gacgtagcga tttgcggcgg tgaccagttc ttgcagtggc gctagagcct
     3661 cccggaagtc gaagcgatcg agcgctcgct cgattcgggc agggatcgta tcggcgatgt
     3721 tgtgcagcac cccgtccgcc tccgccgcgg cgagcgcgga cggcaccacg ccacctcgat
     3781 agcggtgcag catgccaacg gagcggttaa tgaggttgcc gaggtcgttg gcgagatcca
     3841 tgttgtagcg gtcttcgagg cgctggtagg agaagtcgcc gtcgctcgtt cgcggcatgt
     3901 cgcgcagcat ccaccagcgc accgcgtcgg cgccgtactg gttcacgagc acgatcgggt
     3961 cgaccacgtt gccccggctc ttgctcatct tctcgccctc gtgggtgagg aattcgtgga
     4021 cattgacctt cgacggcacc ggcagcccgg cggacatcag cattgccggc cagtagataa
     4081 cgtggaagcg gatgatgtcc ttgccgacca cgtgcacccg gttcgggttg ttgagccagt
     4141 agcggtcgaa gagcgcgccg tcctcggcgt agttcagggc ggtgatgtag ttgctgagcg
     4201 cgtcgaccca gacgtacatc acctggttgg ggtcgtccgg caccggcacg ccccagccgc
     4261 gtccgcgccc ggcctgccga gagatgctga agtcctcaag tccctgattg atgaaggaga
     4321 gcatctcctc gcggcggtgc tccggctgga cgagcatctc gttgctggtg atcagatcgc
     4381 gcaacctgtt ctcatagacc gagaggcgga agaagtagtt ctcctcatcg acgcgatctg
     4441 gatagatgtg gtggatcggg cacttctcgt cgatcagctc ctccggcttg tagaagcgct
     4501 cacagcggac gcagtagagc cccgcgtagt gctgggagta gatgtcgtcg ttgttggcca
     4561 tcgtccgcca gagcttctgc gcgccgggct cgtgccgagg atcggtgctg gtgcgaatga
     4621 agtcatccca gctgaggcgc agtggctcct gcagtgcggc gaaacgcggc gcgttgcggt
     4681 cgaccagctc acgcacgctg acgccctcgc gctcggccgc ctgcacgttg gtgagcgcgt
     4741 tctcgtcggt gccggagaga tagcgggtgt catcgccgtt ctgccggtgg tagcgggcca
     4801 gcacatcgca ctggatcgcc tctagcgcga agccgatgtg gggcatggcg ttcacatatg
     4861 ggatagaggt ggtgatgtag aagcgcttgt cggaagcgtc ggtcatggtg gtcctcctcc
     4921 agacacaaca atcgcgtcac ctgccgcccg agggacgagg gacgcgatcg gtctcgtggt
     4981 accaccctgg ttcgcctgca tctcgcgata cagaccttgg tcactgcgga agaggaccct
     5041 cgaggtgtgt ctggtccgag ggagtgcagt gatcgcccgg taacgggggc gtccggcatc
     5101 gcctactggc gaccgggtgg gtcgggtttg ggatgcggct cggaggtcat ctcggaacgg
     5161 ttccggggcc gtctcgcagc aacacggctc tctgggcccg aaggggcgtt cctacccgtc
     5221 ctcgtcaacg ccggtgttcg gttgtgcgtg tggccgatgg ccacaccaag actctattct
     5281 gccggctcac cgagagtctg tccagttatg gtcgatagcg acgttacgca gaggcctcac
     5341 ctggcccagc cccttgaact gacatcggcc tgcgggcccg ggcagctcac ccggagcctg
     5401 caggccagcg gtacatgttc tggccgcccg atcgaagcgt caggccttgg cggcgtcgag
     5461 gaccgggacg ttcgtcgggg tcgaggccgc gtggccatca gctgtcgccg gcgccgtcgg
     5521 gtcctccgga agctggaccg tccgcccgag atcggctgag aggtacgccg ccttgatgac
     5581 ctggagcgtg cgcgccgtct cgtcgatgtc ggccgggttc gccgcctcgc cggtgacgac
     5641 ctcgatgaaa tgctcgatct ccagcgtgaa gctgtgtacc ggctcgaacg tcttggtcgc
     5701 cgaatccgcc cagccgtaga gctggtgctc caagctgccc ggcccgccac tgagcgcgcc
     5761 cagctcgccc tgcacctcga agtgcttgtt gccgaccacg tccagcgccc agctcgtgag
     5821 gatctccccg accgcgcccg attcgaaccg gacgatcacc atcgcggtgt cctctgtcgc
     5881 cagccccttc tggaagaact tgctgaggat cgctgtcacc tcgaccggac gggagtccgc
     5941 cagcgtgagc agccggtagg tcccgtggta gccggtgtcg atcagctcac cgccgccggc
     6001 ctgcgcgatg tcggaacgcc acccccagtc gcgctgatcg gtctcggcct tgccgaatgg
     6061 ctcccacttc cgcgcttgga aggtctcgat cgagcggagc aggtaggggg tgccaaggaa
     6121 gccgccagcc atcagccgcc gcgcctccag cagggagggc tggaagagtt ggttgtgtgc
     6181 gcccatgaag gtgacccggg tcttctggat cacgtcccgg atgcgggtcg catcgtcgag
     6241 ggtcgtgcag agcggcttct cgcaaagaat cgccttgcct gcctcggccg cggcgatgat
     6301 cgcatcagtg tgcaggtggt gcggcaggca gatgtcgaca acatcgagat cggcctcggc
     6361 gagcatctcg ttgccgctgg tgaaggcgat ggcgctgccg ccggtgtagg tgttgctcat
     6421 ctgctgggcg cgggcggtgg cgatatcggc aaacccgacg atctcggcgc ggtcggggta
     6481 ggtgctccat ccgcgggcgt ggtgccgcgc gatattcccc gatcccagaa tgccgatccg
     6541 aaggcttcgc tgtgccggtg agttcacgtg ggatgctcct gtcaggtgcc gcgcgaggag
     6601 gcgaccggtt cggcgagccc acgcccgccg agccttcgca cgcgccacca tgatttgcgg
     6661 cattgtagcg agtgggtgga tcggtacaag aggaagcggc gccggcgcgc cgggctgcgt
     6721 gatgccgatt ggcgttcagc gaggcgatgc cgcttgcgga cgaatcggcg gtgaagcggt
     6781 tgccagcctg tggtaccagt ggctgaccca gaccaggaac agccccaggg ccacgaagcc
     6841 aatcgtcacg ccgatcacat tcagccccac ggtttcccag ccaccgacct cgccaggatt
     6901 gatgaaatcg tacgggtacc agccgtcgat ctccccacga atgaggctcc acgcgagata
     6961 ggcaattggg tagaccgtcc agaggaacgc ggtcggccaa ctcaggcggt tcgcgaaggg
     7021 gcggaggatg aagtcaagca cgactgctac cgggatcgcc tgatggacca ccgtgtgggt
     7081 ccagtgacgt gtggtgtcga acgggttgtc gaagctgctg accaggaacc cgtaaacgaa
     7141 gaagagggtc accatgtaca tgaccgcggc ac
//
LOCUS       DASYWL010000283         8642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_427729, whole genome shotgun sequence.
ACCESSION   DASYWL010000283 DASYWL010000000
VERSION     DASYWL010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8642
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427729"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..616)
                     /locus_tag="VGR22_10020"
     CDS             complement(101..616)
                     /locus_tag="VGR22_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128943.1"
                     /translation="MAPAGERHRFVNRHDPKGPVYTAGVDFTPKTYADLIEAYDRHPE
                     RKFTGPDGRACQDQTRGLLQDLPLVVSSIRHVGKEGNELEAHRAGVIAEAERQLEYQD
                     SAFVDLVAALQQIPTKVIAEATGYNPRTVRRLKQGEFRPSANRLSQIPTLASRHGIIP
                     RYDGIHSVSGN"
     gene            complement(705..3674)
                     /locus_tag="VGR22_10025"
     CDS             complement(705..3674)
                     /locus_tag="VGR22_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV2128944.1"
                     /translation="MSEPRVTTDIPITHDVETDTDPMVQPQTGEVDPAVTVRIDQFEA
                     LYPFPLDPFQRTAIEILERGDSVMVAAPTGSGKTVVAEYGVYASFRGTGRVLYTAPIK
                     ALSNQKYRDLRGIYGDDVGLLTGDITENREARVLVMTTEILRNMLLQSPWELDDVETI
                     IFDEIHYLADPDRGTTWEESIILCPEHIQLICLSATVTNADEIAGWISRTHRTTRLIT
                     HTARPVPLALYFFHESELHSVVDHTGEHVGDFSRVGGELRRQQGRGRRGRAQMDALEP
                     QPHEIIEQLEKRDLLPTIYFLFSRNDCQAYAERLAALYPLQISEEHKAQIEFIVNTYI
                     DSLREEDRQLEQVSVIRALATKSIGFHHAGLLPVLKQLVEVLFARGLMQVVFATDTLA
                     LGVNMPARTVVVGRMSKWDGRQKRTLIPNEFQQMAGRAGRRGMDIVGHVVMLYSPWIS
                     FRDMLEVATGPMLPVRSAFAIRYNTVLNLWDPPQGERVRQMLQRSLAQYQASQRTRLI
                     EHDILEVESDLQAIPAGCLIGYPEGDALLDDYSARSRLMQSLQTQERRAKRDLRDVQK
                     ATETSSPWPRPARQAIRRTFRTAEPGLVIHHETKGWLVFLGRGEGDAGVGIALRMDDA
                     SLVHMSEYREVDYLTDTIVDIPETLHYPPTAADANAALQETDTGALLATFVNIDVPDL
                     DRLAREHREAEWERRQVEIRGLEEAYEEARSELEGVTAGRESHPCHTCPVRKEHRRYQ
                     RQRDTLEKERAQLIEGLERQSLAEDLRMRNIIRGIRDVLHRFGYLRQGYPTEKADMLA
                     DIFDNDGLILCELIDRGLLDNLSPDELAELFSWFSFDREYRYPNHFLLSNRLLRAREK
                     LEDIEHEVIGEERDHRLFISEGHNPGFYGAAMAWCQGATMAEIGESLELSEGDLVITF
                     NKTIDLLRQVREMLTDVQPDHPLRTRLGEAIERLRKGIVAQSLALGFTPIAEFEDEAD
                     DDEEIVGTEDEDVLDEA"
     gene            complement(4035..4532)
                     /locus_tag="VGR22_10030"
     CDS             complement(4035..4532)
                     /locus_tag="VGR22_10030"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEV2128945.1"
                     /translation="MQRPQPLPVTVGQESVWDYPRPPRMEPDSRRVIVRFNDEVVADS
                     TRTHRVLETSHPPLFYIPPEDVRVELLNANGEQTWCEFKGDAHYYDLAVGDRVSRSAA
                     WCYPEPAKGYEAIQYAIAFYPSRVDECTVDGEIVEAQAGDFYGGWITSEIIGPFKGGP
                     GTQGW"
     gene            4737..5759
                     /gene="thiL"
                     /locus_tag="VGR22_10035"
     CDS             4737..5759
                     /gene="thiL"
                     /locus_tag="VGR22_10035"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEV2128946.1"
                     /translation="MNEQTVRDVGEFGLIDRLVAALPEDVRGTREDVLVAAGDDTAMI
                     RPAAGQVQILTTDTMVEGIHFRLDWTDWRSLGHKALAVNLSDIASMGALPTVAVVTLG
                     LRGDERVSDLEAMYQGLGALARRHGAVVVGGDIVRVNSERLVSVTALGRAGEGEALLR
                     SGARSGDVIGVTGSIGASGAGLAILEDRERYRDSTAGDRLVRAHLTPEPRVDAGQLLV
                     QHGASAAMDLSDGLAGDLPKILAASQVSAEIDAERLPVIAAVRALFSDRWRDLALRGG
                     EDFELLFTIDPKRWDAMASDAKERGITVTRIGTVLPVEGPPELFLIQPSGSRERLPVG
                     AFDHFG"
     gene            complement(5801..7639)
                     /locus_tag="VGR22_10040"
     CDS             complement(5801..7639)
                     /locus_tag="VGR22_10040"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEV2128947.1"
                     /translation="MAAADTIAQHLPRLKGRSYLFGWMGNADRDETGVPPTPPEHCPR
                     CNTILPRTTRSLQLRVCPDCGLHLRLPVRDRIAALVDDGTFRERDAQHASGDPLTFHD
                     ERAYRARLLHQFQLHEHRDAIVTGTAKIGEIPVVIGVLDFAFMGGSMGIVVGEKVAAA
                     AELASEERRPLVTVVASGGARMQEGMFALWQMGRTAAAIKRLRAKGIPYISVLTDPTT
                     GGVYASFASLGDVILAEPEALIGFAGPRVAETMMGEPLPEGSHRAEYLLGHGHVDAVV
                     PRAALRPTISSILGVWQHATRLDDEEVRPQEQWQAKPARQLPDPWALTQDVRLSNRPS
                     SSTYIRQIVSGFVPLAGDRISADDPAVIGGFGRISGLPVMVVGLDRGHDRYPDEAPLR
                     PRPAGFRKANRLMTLAARWRLPLVLFIDTPGAWPGIESEQEGLAGAIAQNLALLSDLP
                     TPTVSVVIGEGGSGGALAFAVADRMLMQERATFSVIAPEGAASILYRDADRAEELARS
                     LRMTARDLEAFGMIDGIIAEPEEGSATDPELAAQMVERAVVSQLDELRRYDTRQLIKY
                     RYERIRRLGDEHIEAPSRRRRVARWLRNKVRSDGPGTPSTVSASSP"
     gene            7909..8481
                     /locus_tag="VGR22_10045"
     CDS             7909..8481
                     /locus_tag="VGR22_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEV2128948.1"
                     /translation="MEDEFLLTALKNRRSISKVSDEEPPEQLIRTMLEAATWAPNHFM
                     TEPWRFFVLRGDARRRLGEVMGEASAAREDDPEKAAASRERMAGKPLRAPYVIAVAVV
                     PDPREKVLEIEEIAATSAAIQNMLLAAEALGLGAIWRTGWVTYTPQVREFLGLSERAT
                     VLGFVYVGYIAKPAEARVRRPADEVTRWLD"
BASE COUNT         1397 a         2726 c         2959 g         1560 t
ORIGIN      
        1 gaagcgagca ggctgaagtt ggacgaggtc gtccgccagc cgtgacggcc gttacaggta
       61 ttggttcact cagttttgcg ggtgcttatc gttcacttaa tcaattcccg gaaactgagt
      121 gaatgccatc ataacgcggt atgatcccgt gccgacttgc tagcgtgggt atctgggaca
      181 gccgattggc acttggccgg aattcgcctt gcttcaatcg ccggacggtc cgtgggttgt
      241 agcccgtcgc ctcggcaatc accttggtcg ggatctgctg gagcgcggcg accagatcca
      301 cgaaggccga gtcctggtac tccagctgcc gctcggcctc ggcgatcacc cccgcccggt
      361 gtgcctcgag ctcgttgcct tccttgccca catgccggat gctggacacg acgagtggca
      421 gatcctgcag caggccccgt gtctgatcct ggcatgcccg tccgtcgggt ccggtgaact
      481 tgcgctccgg atgccggtcg taggcctcga tcagatcggc ataggtcttc ggcgtgaagt
      541 cgacacctgc cgtgtagacc ggccccttag ggtcgtgccg gttgacaaac cgatgacgct
      601 ccccggccgg agccaccaga cagaactgat ccgggcttac gccatccggt cgctgggcgt
      661 atgcattcga gacggccagc ggtgaagacc cgctcgctca tccgctatgc ctcgtcgaga
      721 acatcctcgt cctcagtccc gacgatctcc tcgtcgtcat cggcttcgtc ttcgaactcg
      781 gcgatcggcg tgaacccgag cgcgagggat tgcgccacga tacccttcct gagccgctcg
      841 atcgcctcgc ccagccgggt gcgcagcggg tggtccggct ggacgtcggt gagcatctcc
      901 cgcacctgcc gcagcaggtc gatcgtcttg ttgaaggtga tgaccaggtc gccctcggag
      961 agctcgagcg actcgccgat ctccgccatc gtcgccccct ggcaccatgc catggccgca
     1021 ccgtagaagc cggggttgtg gccctcggag atgaagagcc ggtggtcgcg ctcctcgccg
     1081 atcacctcgt gttcgatgtc ctccagcttc tctcgagcac ggagcagccg gttgctgagc
     1141 aggaagtggt tggggtagcg gtactcacgg tcaaagctga accacgagaa gagctcggcg
     1201 agctcgtcgg gcgagaggtt gtcgaggagg ccccggtcga tcagctcgca gaggatcagc
     1261 ccgtcgttgt cgaagatgtc agccagcatg tccgccttct cggtcggata gccctgccgc
     1321 aggtagccga agcgatgcag cacgtcccgg atgcctcgga tgatgtttcg catccgcagg
     1381 tcctcggcga gtgactgccg ttcgagaccc tcgatcagct gggcgcgctc cttctcgagc
     1441 gtgtctcgct ggcgctgata tcgccggtgc tccttgcgca ccgggcaggt gtggcacggg
     1501 tggctctcac gaccggcggt gaccccctcc agctcggagc gtgcctcctc gtacgcctcc
     1561 tccagtccgc ggatctccac ctgccggcgc tcccactcgg cctcgcggtg ctcgcgcgcg
     1621 aggcgatcga gatcaggcac gtcgatgttg acgaacgtcg cgagaagggc ccccgtgtcg
     1681 gtctcctgca gcgccgcatt ggcgtcggcc gccgtcgggg ggtagtggag ggtctcgggg
     1741 atatcgacga tggtgtcggt gaggtagtcg acctcgcggt actcgctcat gtgcaccagc
     1801 gacgcgtcgt ccatgcgcag cgctatgccg acgccggcat caccctcgcc gcgaccgagg
     1861 aagacgagcc agcccttcgt ctcgtggtgg atgacgaggc ccggctcggc cgtgcgaaag
     1921 gtgcggcgga ttgcctgccg ggccggccgt ggccatggcg acgaggtttc ggttgccttc
     1981 tgcacgtcgc gaaggtcgcg cttcgcgcgc cgctcctgtg tctggaggga ctgcatgagg
     2041 cgcgagcggg cggaatagtc gtcgagcagt gcatccccct cgggatagcc gatcaggcac
     2101 ccggcgggga tcgcctggag gtcgctctcg acctcgagga tgtcgtgctc gatcaggcgg
     2161 gtgcgctggc tcgcctggta ctgcgcgagg ctccgctgga gcatctggcg cacgcgctcg
     2221 ccctgcggcg ggtcccacag gttgaggacc gtgttgtacc ggatcgcgaa ggcggatcgc
     2281 accggcagca tcggtccggt cgcgacctcc agcatgtcgc ggaaggagat ccacggcgag
     2341 tagagcatga ccacatgccc gacgatgtcc atgccgcggc ggccagcccg accggccatc
     2401 tgctggaact cgttcgggat gagcgtgcgc ttctggcggc cgtcccactt cgacatgcgt
     2461 ccgaccacca cggtgcgggc gggcatgttg acgccgagcg cgagcgtgtc ggtcgcgaac
     2521 accacctgca tcaggccgcg cgcgaacagc acctcgacaa gctgcttcaa caccggcaac
     2581 aggccggcgt ggtggaagcc gatactcttc gttgcgaggg cgcggatgac gctcacttgc
     2641 tccagctggc ggtcctcctc ccgcaggctg tcgatgtagg tgttgacgat gaactcgatc
     2701 tgcgccttat gctcctcgct gatctggagc gggtagaggg cagcgagccg ttcggcgtag
     2761 gcctggcagt cgttgcggct gaagaggaag tagatcgtcg gcagcagatc gcgcttttcg
     2821 agctgctcga tgatctcgtg cggctgcggt tcgagtgcgt ccatctgcgc ccgccctcgc
     2881 cggccgcgtc cctgctggcg ccgcaactcg ccgccgacgc gcgagaagtc gccgacgtgc
     2941 tcgccggtgt ggtcgacgac cgagtgcagc tcactctcgt ggaagaagta gagggcaagt
     3001 ggcacgggtc gcgccgtgtg ggtgatcagg cgggtggtgc ggtgtgttcg gctgatccag
     3061 cccgcaatct cgtcagcgtt cgtgactgtc gccgagaggc agatcagctg gatgtgctcc
     3121 gggcagagga tgatcgactc ttcccatgtc gtgccccggt cggggtcggc gaggtagtgg
     3181 atctcgtcga agatgatcgt ctcgacgtcg tccagctccc acgggctctg gagcagcatg
     3241 ttccgcagga tctcggtggt catcaccagc acgcgcgcct cgcggttctc ggtgatgtcg
     3301 ccggtgagga ggccgacatc gtcgccgtag atgcccctca gatcgcggta tttctggttg
     3361 gagagcgcct tgatcggcgc ggtgtagagc acgcgtccgg tcccgcggaa ggaggcgtag
     3421 acgccgtact cggcgaccac cgtcttgccg gagcccgtcg gcgcggccac catcacggag
     3481 tcgccgcgct ccaggatctc aatggcagtg cgctggaagg gatccagcgg gaaggggtac
     3541 agcgcctcga actggtcgat gcgtacggtg acggcgggat cgacctcgcc ggtctggggc
     3601 tggaccatcg ggtcggtgtc cgtctcgacg tcgtgggtga tggggatgtc ggtggtaacg
     3661 cgaggttcgc tcatgttgag tctttctgca ccaaaaccgg atccaatcgc ccgtgtccgc
     3721 gcaagaagcg gaccccgcgg agtgggtgat ccgtagcaga ggggattctt gtcggtatct
     3781 gatcaccggt catggcctgt catcctgagc gaagccccgc aggggcgaag ccgaaggatc
     3841 tcttgccgcc gccatatcat ttccgtatcc caagaagtcc ttcggcctcc gcgcctccgt
     3901 gtcatcttgg ccgcgactcc ggagcatggt cgaagcctga catcagtcct ggaagagatt
     3961 tctcgactcc gcggctgcgc cgctccgctt gaaatgacgg gctggtgtgg agcgaggtcg
     4021 cgacctgaaa ggacctacca gccctgcgtg cccggaccgc ccttgaacgg gccgatgatc
     4081 tcggaggtga tccacccgcc gtagaagtca cctgcctgcg cctcgacgat ctcgccgtcg
     4141 accgtgcact cgtccacgcg gctcgggtag aacgcgatgg cgtactgtat cgcctcgtag
     4201 cctttggctg gctccggata acaccacgcc gccgatcggc tcacgcggtc gccgacggcc
     4261 aggtcataat agtgggcgtc gcccttgaac tcgcaccatg tctgctcgcc gttcgcgttc
     4321 agcagctcga cccggacatc ctccggcggg atgtagaaaa gcggcgggtg gcttgtctcc
     4381 agcacgcggt gggtgcgggt actatcggcc acaacctcgt cgttgaatcg aacgatcacg
     4441 cggcggctgt ccggctccat gcgcggaggg cgcggatagt cccagacgga ttcctggcct
     4501 acggtgacgg ggagcggttg cggacgttgc acgctgcgtt agcctttcgt gagatcgcgg
     4561 aagacgctct ggcctcgaag gatgacaggc aagacaggcg gcttcaacat ggcaagcggg
     4621 tccagagcgg tgagatccgg cagatcagta ggcggtgtgt tttccccgta cacgcctggc
     4681 tttccacagg cgcacacggc agggaccacg cggggcatca accaggagac aaccgcgtga
     4741 acgagcagac ggtgcgcgat gtcggcgagt tcgggctgat cgatcggctg gtcgccgccc
     4801 tgccggagga cgtccgtgga acgcgcgagg atgtgctcgt cgctgcgggt gacgacacag
     4861 caatgatccg cccggcagca ggccaggtac agattctcac aaccgacacg atggtcgagg
     4921 gcatccactt ccggctcgac tggactgact ggcggtcgct ggggcacaag gcgctggcgg
     4981 tcaacctgtc cgacattgcc tcaatgggtg cgttgccgac tgtcgcggtc gtcacgcttg
     5041 gcttgcgggg cgacgagcgc gtgagcgacc tggaggcgat gtatcagggg ctgggcgcgt
     5101 tggcgaggcg acacggcgct gtggtcgttg gtggcgatat cgtcagggtg aacagtgagc
     5161 ggctggtgtc ggtcacagcg ctggggcgag ccggggaggg agaggcgctg ctgcgatccg
     5221 gtgctcgatc gggcgatgtc atcggtgtga ccggctccat cggtgcctcg ggagcggggt
     5281 tggccatcct ggaggatcgc gagcggtacc gggatagcac cgctggcgac cgcctcgttc
     5341 gagcgcatct cacgcctgag ccgagggtcg atgccggcca gctgctcgtc cagcacggcg
     5401 cttcggccgc catggacctc agcgacggcc tggcgggtga tttgccgaag atcctggctg
     5461 cgagccaggt gtcggcggag atcgacgccg agcggctgcc ggtgatcgca gcggtgcgtg
     5521 cgctcttttc cgatcgctgg cgcgacctcg ccctgcgcgg cggggaggat ttcgaactgc
     5581 tcttcaccat cgacccgaaa cgctgggatg caatggcgtc cgacgcgaaa gagcggggca
     5641 tcacggtgac gcgaatcggc acagtgctac ctgtcgaggg cccgccggag ctgttcctga
     5701 tccaaccctc cggaagccga gagcgcttac cggtgggtgc gttcgaccac ttcgggtgac
     5761 ggccggtatc gtcccggttc gagccctgga cggtgagcaa tcagggggaa ctcgctgaaa
     5821 ccgtgctcgg tgtgcctggg ccgtcgcttc ggactttgtt ccgcagccat cgggcgacgc
     5881 gcctccggcg tgacggcgcc tctatatgct cgtctccgag ccgccggatg cgctcatagc
     5941 ggtacttgat cagctgacgg gtgtcgtacc ggcgcagctc atcgagttgg gagacgaccg
     6001 cgcgctcgac catctgcgcg gcgagctcgg gatcggtcgc agagccctcc tccggctcgg
     6061 cgatgatacc gtcgatcatg ccgaacgcct ccaggtcccg tgcggtcatc ctgagcgatc
     6121 gcgccagctc ctcggcgcga tccgcgtccc ggtagaggat cgacgcagcg ccctcaggcg
     6181 cgatcaccga gaacgtcgca cgctcctgca tcagcatccg gtcggccaca gcgaacgcaa
     6241 gagctccacc actgccgccc tcaccgatga cgacgctgac cgtcggtgtc ggtaggtcgg
     6301 ataggagtgc caagttctgc gcgatcgcgc cggccagacc ctcctgctcc gattcgatgc
     6361 cgggccaggc gccgggcgtg tcgatgaaga gcaccagcgg cagccgccat cgagccgcga
     6421 gcgtcatcag ccggttcgcc ttgcggaagc cggcgggccg ggggcgcaac ggcgcctcgt
     6481 cggggtaccg gtcgtgccct cgatcgagcc cgaccaccat caccggtagt ccggagattc
     6541 gcccgaagcc gccgatcacg gccgggtcat ccgcagagat gcgatcgcct gcgagcggga
     6601 cgaagccgct cacgatctgc ctgatatagg tgctgctgga tgggcgattg gaaaggcgga
     6661 catcctgggt cagcgcccat gggtcgggaa gctgccgagc cggctttgcc tgccattgct
     6721 cctgaggtcg tacctcctcg tcgtcgagcc gggtcgcatg ctgccagaca ccgaggatcg
     6781 agctgatcgt cggtcgcaag gctgcccgcg gcacgacggc gtcgacgtgg ccgtgtccaa
     6841 gcagatactc ggcgcggtgg ctgccctctg gcaggggttc gcccatcatt gtctctgcga
     6901 cgcgtggacc ggcgaaaccg atgagcgcct ccggctcggc caggatcaca tcgccgaggc
     6961 tggcgaagga ggcgtaaact ccgccggtcg tcgggtcggt cagcacgctg atgtagggaa
     7021 tccccttggc gcgcaggcgc ttgatggctg cagcggtgcg gcccatttgc cagagcgcga
     7081 acatgccctc ctgcatgcgg gcaccgccgc ttgcgacgac cgtgacgagc ggtcgtcgct
     7141 cctcgctggc gagctcggcg gccgcggcaa ctttctcgcc gacgacgata cccatgctgc
     7201 ctcccatgaa ggcaaagtcg agcaccccga tgacgaccgg tatctcgcct atcttcgcgg
     7261 tgccggtcac gatcgcatcg cggtgctcgt ggagctggaa ctgatggagg agtctcgccc
     7321 gataggcgcg ctcgtcgtga aacgtgagcg gatcacccga tgcgtgctgg gcatcgcgct
     7381 cgcgaaacgt gccatcgtcg accagcgccg cgatgcggtc acgaacgggc aagcggaggt
     7441 gcagcccgca atcggggcac acgcgcagct ggaggctgcg cgtcgttcgc ggcaggatag
     7501 tgttgcaccg ggggcagtgc tccggtgggg tcggtgggac gccggtctcg tcgcgatcag
     7561 cattgcccat ccagccgaag agataggatc ggcccttgag gcgcggaagg tgttgggcga
     7621 tggtgtcggc ggcggccatt cttccagtcc tctacgtgca gccggtgcat gtatacccca
     7681 tggtacggca attcgtggcg aagtttgttc ccgaacgggg atgattcgcc gcaccttcgg
     7741 tacgccgtgc gcgaagaggg gagcgatcag catgaggcgc gcggataaat ctgataggat
     7801 tggtcgggtt tgaggcgggt aagccggtcg cctcctgatg ttccacagaa aaccgggtaa
     7861 agcgtgaggt tgatcttgca gaatccgcat gttagcacag agctggtgat ggaggacgag
     7921 tttctcctga ccgcactcaa gaaccgccgc agcatcagca aggtctccga cgaggagccg
     7981 cccgagcaac tgatccggac gatgctcgag gcggcgacgt gggctcccaa ccatttcatg
     8041 accgagccgt ggcgcttctt cgtgctgcgc ggcgacgccc gcaggcgtct tggtgaggtg
     8101 atgggcgagg ccagtgccgc acgcgaggat gacccggaaa aggcggcagc gagccgcgag
     8161 cgtatggctg gcaaaccgtt gcgcgcaccc tatgtgatcg ccgtggccgt cgtgccggac
     8221 ccgagggaaa aggtgctcga gatcgaggag atcgccgcca cctctgcagc catccagaac
     8281 atgctcctcg ccgccgaagc gctcggactc ggtgccatat ggcgaaccgg atgggtgacc
     8341 tacaccccgc aggtacgcga gttcctcggg ttgagcgagc gtgccaccgt gctcggcttc
     8401 gtctatgtcg gctacatcgc caaacccgcc gaggcgcgcg tgcgcaggcc cgcagatgag
     8461 gttacccgct ggctcgatta gggagcgatg tgcacgagtt cccagaccac gccttgacgg
     8521 atcgtcgggc aggtgcctat gctctcaata ccgactatac cagtcggaat tggatacaag
     8581 cccggtgggc agccccgggc ggtcgagcat gaggagttct ttcacgatga cccacgtgca
     8641 ga
//
LOCUS       DASYWL010000284         6144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_33107, whole genome shotgun sequence.
ACCESSION   DASYWL010000284 DASYWL010000000
VERSION     DASYWL010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6144
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33107"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VGR22_10050"
     CDS             <1..263
                     /locus_tag="VGR22_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128949.1"
                     /translation="GPNAVVLSTPTPPSVETLRDTVTCLRAIETSGTEPLIGYGGAVF
                     EEQPELRDTVDALYLGKDARRAQERLEQALHTRYQEVAYSRA"
     gene            841..1761
                     /gene="murQ"
                     /locus_tag="VGR22_10055"
     CDS             841..1761
                     /gene="murQ"
                     /locus_tag="VGR22_10055"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF009222.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEV2128950.1"
                     /translation="MTDQRTGKDLGKLPTEQRNPNTEDIDALPTIDLVRRLNAEDATV
                     AAAVERTLPAIAEAIDRITERIRSGGRLIYIGAGTSGRLGVLDASECPPTFKTPPGLV
                     IGLIAGGDHALRHSIENIEDRTEEGAKALDEIGVSDQDAVVGIAASGRTPFVVGALTH
                     AQTTGATTIALSNVENSAIARHADTEIAAVTGPEPITGSTRMKAGTAQKMVLNMLSTG
                     TMIRLGKTFGNFMVDVQPSNHKLRDRAVRIVAAATETDRGEAADLLLRADNDVKTAIL
                     MGLLDIPADDARRKLESANGVIRDALATAG"
     gene            1758..2672
                     /locus_tag="VGR22_10060"
     CDS             1758..2672
                     /locus_tag="VGR22_10060"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEV2128951.1"
                     /translation="MSTFIGIDGGGTKTDLALIDDGGRLLARLQGDSSNQAAVGFDAA
                     IGVLADLVDRARSEAGIDGPVTAGWIGMAGSDRAEDRIAFRTALGDRFRELRITNDAE
                     LVLSGTPNGSGVALIAGTGSIAFARNEQGITGRSGGWGHVFGDEGSAYTIAVEGLRAI
                     AAETDGRGPASRLTRDLLRWWQAETPPELISRVYRPEVRKADIAASAPVVVAAAEAGD
                     FVACSILDRSGDALASLVTSLLDRIPFAELPTVAGTGGLLLQTTIVRQRLSDRLDAGR
                     VNPKIQLIDDIAASVAHALRRFHAEEEP"
     gene            2669..3820
                     /gene="nagA"
                     /locus_tag="VGR22_10065"
     CDS             2669..3820
                     /gene="nagA"
                     /locus_tag="VGR22_10065"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEV2128952.1"
                     /translation="MNRVRVLRGKALLGLHMEPATVVIDDGHIAAIHRGHVSTDTFAE
                     DVRVDDAHYISPGLIDLQVNGGNGAEIGDSSDAVDHVSAWLPETGVTAWLPTVVTALS
                     DFYAGVFDAWSRVDAEKGAVPLGYHLEGPFISPEKKGAHQLRYIESASEALIERWTAQ
                     DCVRIVTLAPERHGALDHIRQLVEAGVVVSLGHTNATYEEYVAGIDAGATKATHLFNT
                     MTSIHHRTPGAMVAALTDDRVTAGVIPDGIHTHPAMVRLAIDAKGVDRMLIVTDMMAA
                     AGLEPGTYGLGGQEVSVDETTARLDDGTLAGSVITMDAAIRNILGWSHASLAETLYMA
                     TAVPARVIGERDRGLLHAGALADIIVWDRDLNVQETIIGGQSIYRRGTN"
     gene            4026..4349
                     /gene="gatC"
                     /locus_tag="VGR22_10070"
     CDS             4026..4349
                     /gene="gatC"
                     /locus_tag="VGR22_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEV2128953.1"
                     /translation="MGLSLKDVQHVAALARLGLSDEEMEILRDQLSSILEHIEVLNRL
                     DTENISPTAQVIALDNVMRDDTVAPSLDQDVVTTLAPRSHNGFIAVPAVLGTVDGVEG
                     DSACE"
     gene            4342..5802
                     /gene="gatA"
                     /locus_tag="VGR22_10075"
     CDS             4342..5802
                     /gene="gatA"
                     /locus_tag="VGR22_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEV2128954.1"
                     /translation="MSELTRITAAEARQLLDSKTITAAELTEAHLARIEQIDGIVHGY
                     LHVMDQISREQARQADERIASDAAGPLTGIPVALKDILVTTDAPTTAASRILEGYVSP
                     YDATVVRRLREAGAVFLGKTNTDEFAMGSSTENSAFGSTRNPWKSTTVPGGSSGGSSA
                     VVAAQEATLSLGSDTGGSIRQPAGFCGVVGMKPTYGRVSRYGLIAFASSLDQIGPFSR
                     TVSDAATLLTAIAGHDPSDSTSITIDVPNFAADLPYELKGKKIGIAKEYTVDGMEPGV
                     QAAVDRAIKQLGELGAEMVEVSLPHTEYALATYYITAPAEASANLARYDGIKYGNRKE
                     GETLQETYLRTRGEGFGPEVKRRIMLGTYALSSGYYDAYYVKAQKVRTLIKRDFDEAF
                     EKVDAIVAPTSPTVAFNIGERVDDPYAMYLADVFTIPANMAGIPGISVPCGFSDGLPV
                     GLQFLGKAFDERTVLEAAHAYEQATDWHAKEPPILG"
     gene            complement(5944..>6144)
                     /locus_tag="VGR22_10080"
     CDS             complement(5944..>6144)
                     /locus_tag="VGR22_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128955.1"
                     /translation="VEALGKAGIAAGADGLIIEVHPNPDQAMSDGAQSVDFAQFGAMM
                     PKLARVAEAVERVAPRMTAEVA"
BASE COUNT         1182 a         2075 c         1929 g          958 t
ORIGIN      
        1 ttggaccgaa tgctgtcgtg ctctccacac cgacaccgcc ttcggtcgag acgctccggg
       61 acaccgtcac ctgcctgcgc gcgatcgaaa catccggcac cgagcccctg atcggctacg
      121 gcggggcggt gtttgaggaa cagccggagc ttcgggatac ggtggatgcg ctatatctcg
      181 ggaaggacgc gaggcgggca caggagcggc tcgagcaggc gcttcacacc cggtaccagg
      241 aagtggcgta tagcagggcg tgacaccgac cgcagggctc aaaccgtttc tccgtaagcc
      301 gcctgctgca tggggccggg tcaaagcgcc ggcttgcccg acgcttgacg ccttgcacct
      361 ccaccgccac ttgcctggca aggaacaacc tgttccgaag ggcgcggtac cgtcgttcca
      421 agaacatcgg cgtgccacga atcaccaatc ctgcctccaa acagaatgaa catcggcgga
      481 tcagtcggct acctgttcca accaatgacc ttcttgccat ccttttccac gcacgagggc
      541 agaatctgca gcggagccga gacccgtccc gtgagcgccc accacccgag catcagaatg
      601 ccgggcccga aggtgctggc gatagcttgg gaggtcgcag cgcgagctct tcctgaacac
      661 gacttggaga agagcgcttc atggatctcc tcggatgcac ccctaacctg aacgaaacaa
      721 tcgataatgt cggcaacact gacgcctcgt cgattgctgg cgcatgcaaa gctgcgcgtt
      781 cgtcaattcc gtgggcggtg gcctcatcta ccgcccgttg acccagcagg agaaagcaag
      841 gtgaccgacc agcggactgg taaggacctc ggcaagcttc caaccgagca gcggaaccct
      901 aacacggaag atatcgatgc cctgcccacc atcgatctcg tccgccgact caacgcggag
      961 gacgcaaccg tcgccgcggc cgtcgagcgg acgctcccgg cgatcgctga ggcgattgac
     1021 cgcatcaccg agcgaatccg cagcggaggc cggctcatct acatcggcgc cgggacgtcg
     1081 ggccgtctcg gcgtgctgga cgcatccgaa tgcccaccga ccttcaaaac cccgcctgga
     1141 ctggtcatcg ggctcatcgc cggtggcgac cacgcattgc ggcactccat cgagaacatc
     1201 gaggaccgta ccgaggaggg cgcaaaggcg ctcgacgaga tcggcgtcag cgatcaggac
     1261 gcggtcgtcg gaatcgcggc gagcggccgt acaccattcg tcgtcggcgc cttgacgcac
     1321 gcgcagacga ccggggcgac gacgattgca ctcagcaatg tcgaaaacag cgccatcgca
     1381 cgacatgccg ataccgagat cgccgcggtg accggcccgg aaccgatcac cggctcgacg
     1441 cgtatgaagg ctggcacggc gcagaaaatg gtgctcaaca tgctctcaac gggcacgatg
     1501 attcgattgg gaaagacctt cgggaacttc atggtcgacg tgcagccctc gaatcacaag
     1561 ctgcgggacc gcgccgtccg gatcgtcgcg gccgcaacag agaccgaccg tggcgaggcg
     1621 gccgacctgc tgctccgcgc cgacaacgat gtcaagacag cgattctgat gggcctgctc
     1681 gatatcccgg ccgacgacgc gcgcaggaag ctggaatcag ccaacggcgt gatccgcgac
     1741 gcactggcta ccgccggatg agcacattca tcggcatcga cggcggaggc accaagacgg
     1801 acctcgccct catcgacgac ggcggccgcc tgctcgcacg gctccagggc gatagctcca
     1861 atcaggcggc cgtaggtttc gacgctgcaa ttggcgtgct ggccgacctc gtcgaccgcg
     1921 cccgcagtga ggccggcatc gatggacctg ttacggccgg ttggatcggc atggctggca
     1981 gcgatcgcgc cgaagaccga atcgccttca ggaccgcact cggcgatcgc ttccgtgaac
     2041 tgcgaattac caatgatgcc gagctggtac tctccggcac gccgaacggg agcggcgtcg
     2101 cgctgatcgc aggcacaggc tcgatcgcct tcgcgcgcaa cgagcagggc atcacagggc
     2161 gctcgggcgg ctgggggcat gtcttcggtg acgagggcag cgcctacacg atcgccgtag
     2221 aggggctccg agcaatcgcc gccgagacag acggtcgcgg gccggcaagc cggctcacgc
     2281 gcgacctgct ccggtggtgg caagccgaga cccctcccga attgatctcg cgcgtgtaca
     2341 ggccggaggt gcggaaagcg gacatcgccg cctcagcgcc ggtggtcgtg gcggccgccg
     2401 aggccggcga tttcgtcgca tgctcgatcc tcgaccgatc cggggacgcc ctcgcctcgc
     2461 tggtcacctc gctgctcgac cgcatcccgt tcgccgaact cccgaccgta gccggaacgg
     2521 gaggcctgct gttgcagaca acaatcgtgc ggcagcgcct ctcggaccgg ctcgacgctg
     2581 gccgggtcaa tccgaagatc caactcatcg acgacatcgc cgcgagcgtc gctcacgccc
     2641 tgcggcgctt ccacgcggag gaagagccat gaatcgtgtc cgtgtgcttc gaggcaaggc
     2701 cctgctcggt cttcacatgg agccggccac tgtggtcatc gatgacggtc acatcgccgc
     2761 catccaccgg ggtcacgtct cgaccgatac cttcgctgag gacgtgcgag tcgacgacgc
     2821 acactacatc tcccctggat tgatcgacct ccaggtcaac ggcgggaacg gcgcagagat
     2881 cggagatagc agtgatgctg tcgaccacgt ctcggcttgg ctgccggaaa caggggtcac
     2941 cgcttggctg ccgacagtag tcacggcctt gagcgacttc tacgccggag tctttgacgc
     3001 atggtcgcgc gtcgacgccg aaaagggcgc ggtgccgctc ggctaccacc tggaaggccc
     3061 cttcatctcg cctgagaaga aaggtgccca tcagcttcgc tatatcgagt cggcgagcga
     3121 ggcgctgatc gagcgctgga cagcacagga ctgcgtccgc atcgtcacgc tcgcgcccga
     3181 gcggcacggc gccctcgacc acattcgcca actggtggag gccggagtcg tcgtcagcct
     3241 cgggcacacc aatgccacct atgaggagta cgtggcgggg atcgacgccg gtgcgaccaa
     3301 ggccacccat ctgttcaaca ccatgacgag catccatcat cggacaccgg gtgcgatggt
     3361 agccgcgctg accgacgatc gagtcactgc aggcgtcatc ccggacggta tacacaccca
     3421 tccggcaatg gtccgcctcg cgatcgacgc caagggcgtc gaccggatgc tgatcgtcac
     3481 cgacatgatg gccgccgcgg ggctcgagcc gggcacctac ggccttggcg ggcaggaggt
     3541 ctcggtcgac gagacaaccg cgcgcctgga tgacggcacg ctcgccggaa gcgtcatcac
     3601 gatggacgcc gccattcgga acattctcgg atggtcgcac gccagcctgg cggagacgct
     3661 ctatatggcg acggcagtac cggcgcgagt gatcggtgag cgggaccgcg gactcctgca
     3721 cgccggcgcg ctcgccgaca tcatcgtctg ggaccgcgac ctgaatgttc aggaaacgat
     3781 catcggcggg cagtcgatat accggcgtgg cacgaactga acgacagcac acgagccgct
     3841 gtcatccgga tccggaaagg caggagcggg acgcatcggc ggctgcgatc gcgcaccaag
     3901 catgatcacg gtgaagcctc ctgccggagg cttcaccgtg atcatgctag actccggcac
     3961 gaagacacgg ggccgccgcg cgccggggct ccggctgttc tgtgcgcaac gcaatgggag
     4021 gatccatggg cctatcgctc aaggacgtac agcatgtggc cgccctcgcc cgactcggcc
     4081 tcagcgacga ggagatggag attctccggg accagctctc ctcgatactg gaacacatcg
     4141 aggtactcaa ccgcctagac accgagaaca tctcacccac cgcgcaggtg attgccctcg
     4201 acaatgtcat gcgcgacgac acggtcgcgc catcgcttga ccaggacgtc gtgacgacac
     4261 tggccccgcg cagccataac ggtttcatcg ccgtgccggc agtactcggt accgttgacg
     4321 gagtcgaagg ggacagcgcg tgtgagtgag ctaacccgga tcactgccgc cgaggcccgg
     4381 cagctgctcg actcgaagac catcaccgcc gccgagctga cagaagcgca tctggcgcgc
     4441 atcgaacaga tcgacggcat cgtccacggc tatcttcacg tgatggacca gatttcccgc
     4501 gagcaggcca ggcaagccga cgaacgcatt gccagcgacg ctgccggtcc gctgaccggt
     4561 ataccggtcg cgctcaagga tattctcgtt accactgacg cgccgacgac cgccgcgtcc
     4621 agaattcttg agggatatgt cagcccgtac gacgccaccg tggtacgcag gctccgggag
     4681 gccggagcgg tcttcctcgg caagacgaac acggatgagt tcgcgatggg ctcctccacc
     4741 gagaactcgg ccttcggctc gacccgcaac ccatggaaat cgaccaccgt ccctggtggc
     4801 tccagtggcg gctcctcggc ggtggtcgcg gcgcaggagg ccaccctctc gcttggctcc
     4861 gacaccggcg gctcgatccg ccagccggcc ggattctgcg gggtggtcgg aatgaagccg
     4921 acatacggcc gcgtctctcg ttacggcctg atcgcgttcg cgagcagtct ggaccagatc
     4981 ggcccattca gccggaccgt ctccgatgcc gccaccctcc tgaccgccat cgccggccat
     5041 gatccttccg actccacatc gatcacgatc gacgtgccca acttcgccgc cgaccttccc
     5101 tatgagctga agggcaagaa aatcggcatc gccaaggagt acacggtcga cggcatggag
     5161 ccgggcgtgc aggcagcagt cgacagggcg atcaagcaac tcggtgagct cggcgcggag
     5221 atggtggagg tctctctccc gcacactgag tacgcgctcg caacctacta catcaccgca
     5281 cccgcggagg cgagtgccaa cctcgcgcgc tacgacggca tcaaatacgg caaccgcaag
     5341 gaaggcgaaa cgctgcagga gacctatctc cgcacgcgag gcgaaggatt cgggcctgag
     5401 gtgaagcgcc gcatcatgct tggcacctac gcgctgtcga gcggctacta cgacgcctac
     5461 tacgtgaagg cgcagaaggt acgcacgctc atcaagcgcg actttgacga ggcgttcgag
     5521 aaggtcgacg ctatcgtcgc gccgacctcg ccgacggtcg ccttcaatat cggtgagcgc
     5581 gtcgatgatc cgtacgcgat gtaccttgcg gatgtgttta cgatccctgc caacatggcg
     5641 ggcatccccg gaatctccgt gccgtgtggc ttcagcgatg gcctgccggt cgggctgcag
     5701 ttcctcggca aggcgttcga cgagcggacc gtcctcgaag ccgcccacgc ctacgagcag
     5761 gcaaccgatt ggcacgcgaa ggaaccaccg atcctaggct gatcaccgcc cctgcgcttc
     5821 ccacaggata atggcaggcg caccagtggc aacggcggca taacgctgcc acgaccgtgc
     5881 tatatggtcg tggcagcccc ggcggtggtg acgacgtctg tcctgggatt cggggcgcta
     5941 cggtcaggcg acctcggccg tcatgcgggg ggcgactcgc tcgaccgcct cggccacccg
     6001 cgccagcttc ggcatcatcg ccccgaactg ggcgaagtcc accgactgcg ccccgtccga
     6061 catcgcctgg tccgggttcg ggtgcacctc gatgatcagc ccgtccgccc cggccgcgat
     6121 ccccgccttg cccagcgcct cgac
//
LOCUS       DASYWL010000285         2154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_239306, whole genome shotgun sequence.
ACCESSION   DASYWL010000285 DASYWL010000000
VERSION     DASYWL010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239306"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1892
                     /locus_tag="VGR22_10085"
     CDS             <1..1892
                     /locus_tag="VGR22_10085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEV2128956.1"
                     /translation="LGGHQIMRAGLASLRARRVDMNVLMTVSVFGAAILGEWSEGALV
                     VVLFSIGTTLQAVAFDRTRGAIRALLDLTPQEARVVRHGIETTVAVASLERGDIVRVR
                     PGERLPADGEVLTGFSAVDQAPITGESMPVERGEGDEVFAGSLNGSGVLDIRVTKRSS
                     QSMLANIVHLVEGAQSSRAPSQQLVDRFASVYTPIVVALAAAIALAGGGIAGDWGTWV
                     YRSLVLLVIACPCALVISTPVSIVSAIGAATRHGMLVKGGAALEEAGRSRLVAFDKTG
                     TLTLGRPTVTRIVVPEIGSGTYRQSEQDLLALAAAVERDSEHPLAGAVVAKALHDRVP
                     ELPATNFEAFPGRGASSLVDGREIAVGNKRLMIELGVPAGDLVALDVIAEGSLARAGE
                     TALLVAERMPDAWHVLGVIAVADRVRPGAKEAVQRLRDLGIERVVMLTGDRRTVAETI
                     GAQVGVDEIRAELLPHEKAEAIASLQADGWHVTQVGDGVNDAPALATADVGIAMGMGG
                     TDLALESADLALMRDDLWVVGSVIALSRRTVAIIRQNITLSMVTKLIALLLGVLGFVN
                     LWIAVLADVGTSVLVTLNGLRLAHMSDVPAPTASPAEAAVCSCADCGSAERDTAREEL
                     SLPAD"
BASE COUNT          333 a          638 c          779 g          404 t
ORIGIN      
        1 tgctcggcgg ccaccagatc atgcgcgccg gcctggcctc gcttcgcgct cgtcgggtcg
       61 acatgaacgt gctcatgacc gtctccgtgt tcggcgcggc gatcctcggc gagtggtccg
      121 agggcgcgct ggtcgtcgtg ctcttctcga tcggcaccac attgcaggcg gtcgccttcg
      181 accgcacgcg cggcgcgatc cgggcgttgc tcgacctcac gccgcaggag gcccgggtgg
      241 tccgccatgg gatcgagacc accgtcgcgg tggcctcgct ggagcgtggc gatatcgtgc
      301 gcgtccgccc gggcgagcgc ctgccggccg acggcgaggt gctcaccggc ttctcggccg
      361 tcgaccaggc gccgatcact ggtgagtcga tgccggtcga gcgcggtgag ggtgacgagg
      421 tctttgccgg gtcgctcaac ggctccggtg tgctcgatat ccgcgtcacg aagcgatcga
      481 gccagtcgat gctggcgaac atcgtccatc tcgtcgaggg agcgcaatcg agccgggcgc
      541 cgtcgcagca gctcgtggat cggttcgcca gcgtctacac gccgatcgtc gtcgccctcg
      601 cggcggccat cgcgctggcc ggcggcggca tcgccggcga ctgggggaca tgggtctacc
      661 gttcgctcgt gctgctggtg atcgcctgcc cgtgcgcgct ggtgatctcc acgccggttt
      721 cgatcgtctc agctatcggc gcagcaaccc gtcacggcat gctcgtcaag ggaggcgcgg
      781 cccttgagga ggccggacgg tcacgccttg ttgcattcga caagaccggc acgctgacgc
      841 tcggccggcc aacggtgacc cggatcgtgg tgccagagat aggctcaggg acctatcgac
      901 aatccgagca ggatctgctg gcgcttgcgg ctgcagtgga gcgggattcg gagcatccgc
      961 ttgcaggggc agtagtggca aaggcactcc atgatcgggt accggaactg ccagccacga
     1021 acttcgaggc atttccggga cgcggtgcgt cctccctcgt ggatgggcga gagatcgcgg
     1081 tcggtaacaa acgactgatg attgaactgg gagttccggc cggggatctg gttgccctgg
     1141 atgtgatcgc tgaaggatcg cttgccaggg ccggtgagac ggcgttgctc gtggccgagc
     1201 ggatgcctga tgcctggcat gttcttgggg tgatcgccgt cgcggatcgc gtgcgtcctg
     1261 gtgcgaagga ggctgtccag cggttgcgcg acctgggcat tgagcgcgtc gtgatgctga
     1321 cgggcgaccg tcggacggtg gcggagacga taggggctca ggtcggtgtc gatgagattc
     1381 gggcggagct gctgccccac gagaaagcag aggcgatcgc aagcctgcaa gccgacggtt
     1441 ggcacgtgac gcaggtgggt gatggcgtca acgatgcccc tgccctggcc acggccgatg
     1501 ttggtatcgc aatgggcatg ggcggtactg atctcgcact cgagagtgcc gatctcgcac
     1561 tgatgcgcga cgacctctgg gtcgtcgggt cggtgatcgc cctctcgcgg cgaacagtgg
     1621 ccatcatccg gcagaacatc accttgtcca tggtgaccaa actcatcgcg ctgctgcttg
     1681 gcgttctcgg cttcgtgaac ctctggatcg ccgtactggc agacgtcggg acgtcggtcc
     1741 tggtgacgct caacgggttg agacttgcgc acatgtcgga tgtacccgcc ccgaccgcct
     1801 ctccggccga agcagcggtc tgttcctgcg ctgattgtgg ttcagcggag cgtgacaccg
     1861 cgagggagga gctgtcgctc cctgcggatt agcaggtttg gtcgaacaac aagcggtggt
     1921 gtcaccacac agccgcacaa ggtcattcgc cgccgggctc gggactatgc aagtaggagg
     1981 ggtggcccgg aaaagggcat aacggtgacc cataatcgag gaaaatggcg ggctgagttt
     2041 gggagtggga gtacgctggg aattgttcgc ctacggctgc tcgtcgggta cggcacggcg
     2101 gtgtgcaatg ggtgtcgggg ctggagatga ggttggagtc gcgggtgcat attc
//
LOCUS       DASYWL010000286         6221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_71254, whole genome shotgun sequence.
ACCESSION   DASYWL010000286 DASYWL010000000
VERSION     DASYWL010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71254"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VGR22_10090"
     CDS             <1..550
                     /locus_tag="VGR22_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEV2128957.1"
                     /translation="GHEHNLNTRGAFLHVVGDMLGSVGAIVAAVIMLTTGWYLADPIL
                     SAGIGLLILWSSWRLLRESVEVLLEATPAHIDPSEVRAGLTDVDGVAGVHDLHIWTVT
                     SGFIALSAHVEVTRQRDWHDVLVELAGLLREHFGIQHVTLQPENERTGQAFRDCSLDT
                     PEGRAACLSRPVSRREPAHHAH"
     gene            1246..1890
                     /locus_tag="VGR22_10095"
     CDS             1246..1890
                     /locus_tag="VGR22_10095"
                     /inference="COORDINATES: protein motif:HMM:NF025255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4203 domain-containing protein"
                     /protein_id="HEV2128958.1"
                     /translation="MDILIGILTLLLGLAVATMGLRLWFWMLPILGFMVGFSLGTILV
                     YQLAGDGILETVLSWIVGFIAGVAFALASWLWWYLGVIIAAGTAGAALATALAASFGA
                     EANWLLLIIAITGAAVFSIAALLLRLPIYLVIVNTAIAGGLAVVTGLLLIFDRLDLRF
                     LGSGYAFAIIDNSPGWWLLWAIVAGVGIYLQLRETAVVGLPDDRFVPASHAIRR"
     gene            1997..2887
                     /locus_tag="VGR22_10100"
     CDS             1997..2887
                     /locus_tag="VGR22_10100"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV2128959.1"
                     /translation="MSVQPVTPRIDSHHHVWDPATRDYPWLQDLDGINRPFTVDDFRP
                     LLEANGFNGSVLVQTVSDMGETNLFLELATATPFILGVVGWVDLEKLDVDADIRLLRE
                     SPGGNFLVGIRHQVHDEEDPDWLLRAEVLRGLEVVADHELVYDLLVRPRELPAAITVA
                     RDYPHLRFVVDHIAKPPIASGEIEGWASLMHEFAGLENVACKLSGMVTEAGDAGWSVD
                     DLLPYVRTVVEIFGHDRLMFGSDWPVCLLAASYEEVVRATREALERLEVLTPEADAAI
                     FGGTAAHWYNLDIQHLVETA"
     gene            complement(3033..3908)
                     /locus_tag="VGR22_10105"
     CDS             complement(3033..3908)
                     /locus_tag="VGR22_10105"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEV2128960.1"
                     /translation="MNQGIIPGAIGLKDLNLQESVSFAGRHGFDTVMINIREVHEIVQ
                     QHGADYVVDLLAEYGVRPGAWSVPVRWRDDEHRNDDLASLPELMETAHAIGCPRATSG
                     VQPGGDDISLDEQFTWMVDRLRPVAELLETGGIKVGLEFIGTPSYRKERWKNPEFIHD
                     LAGIRALIDNVGRSSLGILFDVWHHWVSGGTPEQIDDLSADEVVLVHVNDAPEGRSLD
                     EQIDNQRTLPMETGVIPVPEMLRRLQAIGFDGPVMPEPFSAGLEALAAENPDDAGTKV
                     KESMDALWKAAGFAV"
     gene            complement(4100..5158)
                     /locus_tag="VGR22_10110"
     CDS             complement(4100..5158)
                     /locus_tag="VGR22_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEV2128961.1"
                     /translation="MTQAEQTQGTADDTVTATGTRTIGGASRTPDLAALPVTLDDVIA
                     ARERIMPHLHRTPALNTATLGGMTGTDLALKCETFQRTGSFKARGAINAILQLTPEQR
                     ERGVVTLSAGNHGQGLAFAASKVGTRAVVFMPETAVPAKVDAIRGYGAEAMFAPTMET
                     LFAAMTAYKEEHGLTFVPPFSDPAIVAGQGVVGLEILEDVPDVETIVVPAGGGGLLAG
                     ILVAVKSQRPNVRIVGVEPTGANIVFRSREEGRLVTPEKIETIADGLAAPFAGEVTQP
                     IVDTLVDDMVLVEDEQILRALQLIIERCKIVVEPAGAAATAALLTGKIPDARGTRTVT
                     LLSGGNIDLNKLAHFLTA"
     gene            5289..5660
                     /locus_tag="VGR22_10115"
     CDS             5289..5660
                     /locus_tag="VGR22_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128962.1"
                     /translation="MPDWQPTDTADHPKAATVRTVPLDEGALNAITETLGGLCELADF
                     RLPASRVWRITVSGADDRPAAMLTIWPGIGRVDAVNGMATVVFSGVQTVALVPGVEAQ
                     FRRGSREVLIVARGGKVIVRA"
BASE COUNT          988 a         2105 c         2001 g         1127 t
ORIGIN      
        1 cggccacgag cacaacctca acacacgcgg cgccttcctg cacgtcgtcg gcgacatgct
       61 cggctccgtg ggggcgatcg tcgcggcggt catcatgctc acgacgggct ggtacctcgc
      121 cgacccgatc ctctcggccg gtatcggcct gctcatcctg tggagctcgt ggcggctgct
      181 gcgggaatcg gttgaggtgt tgctggaggc gacgccggcc catatcgacc cgtccgaggt
      241 tcgtgccggg ttaaccgatg tcgatggcgt cgccggcgtg catgacctgc acatctggac
      301 ggtgacgtcc gggttcattg cattgagcgc gcacgtcgag gtcaccaggc agcgcgactg
      361 gcacgacgtg ctggtggagt tggccgggct gctgcgggag cacttcggca tccagcacgt
      421 cacgctgcag ccagagaacg agcggaccgg tcaggcgttc cgcgactgct cgctcgacac
      481 ccccgagggc cgtgccgcct gcctctcccg gcctgtctca cggcgggaac cggcccacca
      541 cgcacactga tccgatggaa ctggccagac cctcgcagcc cttgtcattt caaccggagc
      601 gaggggaaag cgcatgtagc gggaaaatcc ttcctcgcgg acacaggcat tccgaacgca
      661 gagcattgct cgacgcggcc ctgcgccccg gctcgaacca gcacagctga gatgcgttgc
      721 cctcactgca gcccggtatg ggcgcattct ggacagccga tgcctcggca ggccgggcag
      781 tcggcgccga gcatgcggac gagcgtgaca ggcaaccggt gccgaggggt gagcgaggtt
      841 cggtcgctga gctgccgagt cggcaccggg tcgatcgata ctgagggaag gcaagcggcg
      901 gacctgtgcc gtgtggcgac atcggtgcgg tcgagagtgc ctcgcagcag tcgccgcaac
      961 tgggtgatgg ccggcaccgt tcgctccttt ccgtggtctg gttccgggga gccggatggg
     1021 tccggcccca cgtccatgat gcgtcgtcca taagccggta tcgacgggca catcgcacaa
     1081 aatgcacggc ccgagtggtg tgccgtgcat agtggcaatg tctggctatc gccatcggtt
     1141 tcaggtgcag tggccggcga cgccgatcgt cgcaggaaat ggcacgaacc ctgctaccat
     1201 gcttgcgccc gactcggtcg tcgcccgtgc ctgaaaggtc cgtgcatgga tatcctgatc
     1261 ggcatcctga ccttgctcct cggcctcgcc gtcgcgacga tggggctgcg gctctggttc
     1321 tggatgctac cgatactcgg gttcatggtc ggcttttcgc tcggcaccat cctcgtctac
     1381 cagctcgccg gcgacggcat cctcgaaacg gtcctcagct ggatcgtcgg attcattgcc
     1441 ggggtcgctt tcgccctggc ctcctggctc tggtggtacc tcggcgtgat catcgctgcc
     1501 gggacggccg gagccgcgct cgcaaccgcc ctcgctgcat ccttcggcgc ggaggcgaac
     1561 tggctgctct tgatcattgc gatcactggc gcggccgtgt tctcgatcgc ggcgctcctg
     1621 ctccgcctgc cgatctacct cgtcatcgtc aacacggcca tcgccggggg acttgcggtg
     1681 gtcaccgggc tgctgctgat cttcgatcgg ctcgacctcc gcttcctcgg cagcgggtat
     1741 gccttcgcca tcatcgacaa ctcgcccggc tggtggctgc tctgggccat cgtcgccggc
     1801 gtcggcatct acctccaact ccgcgaaacc gctgtcgtgg gcctgcccga cgaccggttc
     1861 gtcccggcga gccatgcgat ccgccgctga ccgtcaggaa tccggacttt acgagccggg
     1921 tggattcgct atgctgaagg ttccggatcg atccctggct tgacggggat ctttttctcg
     1981 ccaccgggag gagcgtatga gcgtccaacc tgtcacccct cgcatcgaca gccaccacca
     2041 cgtctgggat cccgccacgc gcgactaccc atggttgcag gatctcgatg gcatcaaccg
     2101 cccattcacg gtcgacgact tccgcccgct gctggaggcc aacggcttca acggcagcgt
     2161 gctcgtccag accgtctccg acatggggga gaccaaccta ttcctcgagc tagccacggc
     2221 gacgcccttc atcctcggcg tcgtcgggtg ggtcgacctc gagaagctcg acgtcgacgc
     2281 cgacatcagg ctgcttcggg agagtcccgg cgggaacttc ctcgtcggca tccggcatca
     2341 ggtccacgat gaggaggatc ccgactggct gctgcgcgcg gaggtattgc gtggcctcga
     2401 ggtggtcgct gatcacgagc tggtctacga cctgctggtg cgcccacgcg agctgcccgc
     2461 cgcaatcacc gtcgcgcggg actatccgca cctgcgcttc gtcgttgacc acatcgccaa
     2521 gccgccgatc gccagtggcg agatcgaggg gtgggcatcg ctgatgcacg agtttgccgg
     2581 gctggagaat gtcgcctgca agctctccgg catggtcacg gaggcaggcg acgcgggctg
     2641 gagcgtcgac gacctgctgc cctacgtgcg gacggtggtc gagatcttcg gtcacgatcg
     2701 gctgatgttc ggctcagact ggccggtctg cctgctcgcc gccagctacg aggaggtcgt
     2761 ccgcgccaca cgggaggcgc tggagcggct ggaggtgctc accccggaag ccgacgccgc
     2821 catcttcggc ggcaccgctg cccactggta caacctcgac atccagcacc tcgtcgagac
     2881 cgcctgacac ggaagtatgg cttagagcac cagtgtccgt gtatcgttcg ttgtcgtcaa
     2941 ctggatagcc gctggtcgag caggaaaacg ggcggacaca aggtccgccc ctaggagtcg
     3001 gcgggtttgc ccttgttcat ccggatcctg tatcagacgg cgaagcccgc cgccttccaa
     3061 agggcatcca tcgattcctt gaccttcgtc cccgcgtcgt cggggttctc agcagcgagc
     3121 gcctcgaggc cggcgctgaa cggctccggc atcaccggac cgtcgaaccc gatcgcctgc
     3181 agccgccgga gcatctccgg caccgggatc acccccgtct ccatcggcag cgtgcgctgg
     3241 ttgtcgatct gctcgtccag cgagcgcccc tccggcgcgt cgttgacgtg caccagcacc
     3301 acctcgtcgg cggagagatc gtcaatctgc tcaggggtgc cgccagagac ccagtggtgc
     3361 cagacgtcga agagaatgcc gaggctgctg cgaccgacgt tgtcgatcag cgcgcggatg
     3421 ccggcgaggt cgtggatgaa ttcagggttc ttccatcgct ccttgcggta gctcggcgta
     3481 ccgatgaact cgagcccgac cttgatgcca ccggtttcca gcaactcggc caccggtcgc
     3541 agacggtcga ccatccaggt gaattgctcg tccagggaga tatcgtcgcc ccccggctgg
     3601 acaccggagg tcgcacgagg gcagccgatc gcgtgggcag tctccatcaa ctccgggagg
     3661 gaagcgagat catcattgcg atgctcgtcg tcccgccagc ggacaggcac actccatgcg
     3721 ccaggtctga cgccatactc ggctaagaga tcgaccacat agtcggcacc gtgctgctgg
     3781 acgatctcgt ggacctcgcg aatgttgatc atcaccgtat cgaagccgtg tcgaccggcg
     3841 aaggagacgc tctcctgaag attgaggtcc ttgagaccga tcgcgccggg gatgatgcct
     3901 tggttcatgt actgcgaatc ctttcgagat gatcgtcaca atgggtgctg gccggccctt
     3961 cgtcatgcaa gcagggccgg acgcacgccc ccgatcgcct gggtgagcgt gtgtcgtgga
     4021 cagggtctgc gaactgacac gcgcgcgcaa gaggcagcga gcggtgcccc cggctgatcc
     4081 gtccagagag ctcttgcgat caggctgtca ggaagtgcgc gagcttgttg aggtcgatgt
     4141 tgccgccact caggagcgta accgtccgcg tgcccctggc atccgggatc ttgcccgtca
     4201 gcagtgccgc cgtcgcagcc gcccctgccg gttcgacgac gatcttgcag cgctcgatga
     4261 tcagctgcag cgcccgcagg atctgctcat cctccaccag caccatatcg tcgacgagtg
     4321 tgtcgacgat cggctgggtc acctcgcccg cgaatggcgc cgcgagccca tccgcgatgg
     4381 tctcgatctt ctccggcgtc accaggcggc cctcctcccg gctgcggaag acgatgttcg
     4441 ccccggtcgg ctcgacaccg acgatccgga cgttcggccg ctggctcttc accgccacga
     4501 ggatcccggc aagcagcccg ccgcccccgg ccggcacgac gatcgtctcg acgtccggca
     4561 cgtcttcgag gatctcgagc ccgaccactc cctgtccggc gacgatcgcc gggtcggaga
     4621 agggcggcac gaaggtgagc ccgtgctcct ccttgtaggc ggtcatcgct gcgaagagtg
     4681 tctccatggt cggcgcgaac atggcctcgg caccgtagcc gcggatcgcg tcgaccttgg
     4741 ccggtaccgc ggtctccggc atgaagacga ccgcccgcgt cccgaccttg gaggcggcga
     4801 aggccagtcc ctgcccgtgg ttgccggcag agagggtcac cacgccgcgc tcgcgctgtt
     4861 ccggggtgag ctggaggatg gcgttgatcg cgccacgcgc cttgaacgat cccgtgcgct
     4921 ggaaggtctc gcacttgagc gcgaggtccg tgcccgtcat gccgcccagc gtggcggtgt
     4981 tgagcgccgg ggtgcggtgc aggtgcggca tgatccgctc gcgggcagcg atgacgtcgt
     5041 cgagcgtgac cggcagcgca gcgaggtcgg gagttcgact ggcaccgccg atcgtgcggg
     5101 tgccggtcgc cgtcaccgtg tcgtctgccg tgccctgcgt ctgctccgcc tgcgtcatgc
     5161 cagtatcctt ggtcgtccct gatcgatgat gcacccctcc cctgtggaag ggcgcctaca
     5221 ctggcgaaga gtataccgga gggctgggca cgcgccctcg acatgagatc gacccgagga
     5281 gtcaccccat gcccgactgg cagccaaccg acaccgccga tcacccgaag gccgccactg
     5341 tacggaccgt cccgctcgac gagggggcgc tgaacgcgat cacggagacg ctcggcggct
     5401 tgtgcgagct ggcagatttt cgactgccgg cgtcgcgcgt ctggcgcatt acggtctccg
     5461 gcgcagacga ccgacccgcg gccatgctga cgatctggcc gggcatcggc cgggtcgatg
     5521 ccgtcaacgg catggcgacg gtcgtcttca gtggcgtgca gacggtcgct ctggtacccg
     5581 gcgtcgaggc gcagttccga cgaggcagcc gagaggtgct gatcgtcgcc cgtggcggca
     5641 aggtcatcgt tcgcgcgtaa cgcgacggtg acgatcgcct ctcaatctcc ctggcagctg
     5701 acccgagcgt gggcaacgcc gttcctgagg ggacgcgcgc attgccaata tcgcctgtga
     5761 cagtgtgaag gctgctaccg atccagtggt ctcgccccgc tgctggcaca ggccttgcac
     5821 cctttagcgt gaggtcggcc cacagctatc gtcctggcac ggcctccata tcacaccctc
     5881 atcctgaaat ggaggccacc atgaccctct cctacccctt gttcgaactc ggaattgtca
     5941 cagccctgac ggccggatgg gtcatctgga gcttcctgac ccactcccac cggcgccagc
     6001 ttgtccgcca accctcctac gcacgcagag cggcacgtcc acgccggccg aaccaggctt
     6061 gaccatcaca ctgcatgcgg ccagcggtag cgatcgttga ccgggttcct ggagacggcc
     6121 agttctcgat caccaccgaa ttcagttcgg caccgcaaca aggtcttctt gagtcaattg
     6181 catccggtgc atgcagtttc tgcaggtaca acccaggaca t
//
LOCUS       DASYWL010000287        14071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_653007, whole genome shotgun sequence.
ACCESSION   DASYWL010000287 DASYWL010000000
VERSION     DASYWL010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 14071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14071
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653007"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            385..1098
                     /locus_tag="VGR22_10120"
     CDS             385..1098
                     /locus_tag="VGR22_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128963.1"
                     /translation="MVFLFSWRTTSMILGLLPLAFVLALPVTEAQASQATPPATSTSA
                     TPTPMPTEELTTPEPPITGDPNACVDGRLHISDLANADLHLQEGLHRVSKRAEMWQSD
                     AQLVELRLACPLLKTGLQWEGTFFSPSAQANFSTDTFDVEPSEEAPDEVPYLDVANIS
                     FQAVHRSLLRAGYSDDLRLAAASSVTIRRSTEQTPFGPPIAPPGVVYVHVAVEERGVI
                     KDVWVNAEDGTIYRYDLGG"
     gene            1103..1945
                     /gene="otsB"
                     /locus_tag="VGR22_10125"
     CDS             1103..1945
                     /gene="otsB"
                     /locus_tag="VGR22_10125"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEV2128964.1"
                     /translation="MANRPIEADVASAVDHTVAVLAKRPAGLLFDYDGTLSELVNDPT
                     LAAIERRSEAAIHELTDLVDVVAIVTGRAARDVQTRLDTSDLVVVGNHGFEWVVQDVH
                     TEHDAGVDAANAVENALAEIEERLTRDNLVEGVIFENKRLSGSIHYRQAPNQRKVGSV
                     LIPLARKIAAAHNLRPTEGKMIVELRPIAEVNKGTAMYHIRDEFNLAGMAFIGDDLTD
                     VDGFESLRDIRNAGVADTVAVGVLGPDTHPSIALTADVAVTGVLGVADYLERLVARLR
                     EVVS"
     gene            complement(2034..2672)
                     /locus_tag="VGR22_10130"
     CDS             complement(2034..2672)
                     /locus_tag="VGR22_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HEV2128965.1"
                     /translation="MSTNDTALLVAGIIDEFESINAAREKPISQSRHVIRASADAIRA
                     AHRGEFDDARSGAGEASGILQEIVDHVTDRPSPAGAGYVQDAMKEVAEAHISIAILSG
                     SDLPAAGDLGVSSSAWLNGLAEAASELRRDALDALRYSDVPRAESLLQQMDTIYSSLV
                     TVVFPDAITGGIRRTTDQLRAVLERTRGDITFDLRQDRLERMLRQTESRLRR"
     gene            2983..3058
                     /locus_tag="VGR22_10135"
     tRNA            2983..3058
                     /locus_tag="VGR22_10135"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3016..3018,aa:Ala,seq:ggc)
     gene            3184..5670
                     /gene="leuS"
                     /locus_tag="VGR22_10140"
     CDS             3184..5670
                     /gene="leuS"
                     /locus_tag="VGR22_10140"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEV2128966.1"
                     /translation="MATTTTDTRRDIERTWNPAEIEPKWRERWQEAAIYQVDDNAPEP
                     KWYSLTMYPYPSGTLHVGHWYAFVGPDIYARAKRMQGYKVLFPMGFDAFGLPAENAAI
                     RHNIHPATWTYENIEQMIAQFDLMGPMIDWSRKLITSDPEYYRWNQWIFLQMLERGLA
                     YRAAGSVWWCPNDQTVLANEQVVEGNICERCGAEVYKRDLEQWYFRITEYAEELLAEL
                     DNLDWPEPVKVMQRNWIGRSEGARLSFRLETGNELEVFTTRPDTVFGATFMVLAPEHP
                     LVDKITTDEHREAVGAYRGEARRKSEIERQSTDEAKEKTGVFTGASAVNPVTDERIPV
                     WIADYVLMSYGTGAIMAVPAHDERDFAFARAFDLPIRVVIQPEGEDALDPRTMEAPYT
                     GAGVMVNSGQFDGTPTPEQLPKVIAWLNEQGRGQAEVTWRLRDWLISRQRYWGTPFPI
                     VYCDDCGMVPVPEDQLPVELPLDAEFTPTGQSPLVNHERFLNTTCPSCAGTARRETDT
                     MDTFVDSSWYWFRYTNPNFDDAPIDEERTKLWTPVDIYTGGIEHAILHLLYARFFAKV
                     LRDLELVDHGEPFKTLRNQGIILAAEGTKMSKSRGTQVGPDELVAAHGADALRLHLMF
                     LGPWEQGGPWNERGITGMERFIRRAFQVVTETAGNRMDVKADAADLRRLTHKTIARVT
                     RDLGQFQFNTAIAALIEMTNELMKLKETEVAETTAWRDAITALTLLMAPLTPYTAEEM
                     WEMLGNEFSVHQQDWPVYDESLAAVETVEIPVQVNGKVRERVAVAVDASEEDVTAVAR
                     ETGKIPDYLQGKTLKRTIYVPGRLINFLVG"
     gene            complement(5926..7401)
                     /gene="gcvPB"
                     /locus_tag="VGR22_10145"
     CDS             complement(5926..7401)
                     /gene="gcvPB"
                     /locus_tag="VGR22_10145"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEV2128967.1"
                     /translation="MAVESLIFELSSPGRRAVRIPEPDVPETEIPASLRRNELNWPEV
                     SEIDVVRHFTRVSQKNHAIDINMYPLGSCTMKYNPKVNERVARLPGFANIHPKAPEET
                     VQGALELMHELQNILAEISGFEAVTLQPAAGALGELTGCLMIKAWHKDHGGENRTKML
                     IPDSAHGTNPATARMAGFDVVTVPSDNRGNTDIDAVRELVGPDTAGLMITNPNTLGLW
                     DEHIQEMIQIVHEAGGLVYNDGANFNAILGIASPGDMGIDVMHFNLHKTFSTPHGGGG
                     PGSGPVGVTKELAEYLPGPIVTRVDEEDGPRFAWKQPERSIGKLHAFNGNFGMHIRAY
                     TYIRMHGEDGLRQVSVDAVLNANYLRARLHEAYHVPYDRTCMHEFVATASHQKKNGVR
                     TVDIAKRLLDFGVHPPTTYFPLIVPEALMIEPTETESRESLDYFVDAMLQIAEEAESD
                     PEKVKHAPHTTEYKRLDEVGANRNLNLRWIPERQKEAVAAD"
     gene            complement(7738..8940)
                     /locus_tag="VGR22_10150"
     CDS             complement(7738..8940)
                     /locus_tag="VGR22_10150"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2128968.1"
                     /translation="MRLRWDVGLGRDLGLAFWAMTFFEATFGAYMGVWPLWIEELGAP
                     IALVGLVLGSAGFIRPLVIGPSSWLTERVDRKPLMIAARISGIAGMVVAALAQAWPIL
                     FITVVLNAIGEMVFPLLQTYVAERAHEDRARAFSMIINVGPSVALMITPLLSGALIAQ
                     WGMRSAFVLGAITGLASILILSRMDLSLPRDEDDGEAAQPNYGDTFANSSIRPLFAVH
                     GVTILSLAIGISLIPVFLRDTRGLDPALIATLGAGSALGTALFGLAVARTPTLSNSPM
                     RAATISCAAIVAGLFVFTFAEALPLIALAFFLRGGMFATWAMILAEMGNIAPTRLRSR
                     AFAVLEMLGGSAMSFGPIIASQLYGISPSVPIFTGAVLGGIMAVVMGWMQWRRSDHTS
                     ADTTAIPV"
     gene            9057..9722
                     /locus_tag="VGR22_10155"
     CDS             9057..9722
                     /locus_tag="VGR22_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128969.1"
                     /translation="MGRGVRSRVFPTLPRGTVVLPVDAYLLTGLAMCAVLLVALAGTA
                     YLAVFFNRRAKDDLTAAFTPLAKRMDGSFELDNAEIRGAYQNYPVFARMANATEGPGR
                     VFQVDVLDAAGGDLWQYTSNPPTKREPERTVEFEGDDEIRQIVKPAIETESRRFLDPD
                     IERFRVEYLPREGVVRLVRAMRSRRDIPDAGTLASELDMLTGIAGRNRAWVESRNGGE
                     VRA"
     gene            9719..10492
                     /locus_tag="VGR22_10160"
     CDS             9719..10492
                     /locus_tag="VGR22_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128970.1"
                     /translation="MTRAIVYVDSAEEQVAYLIARFGPQGAWSVREQRVATLDNGASV
                     ERASLQTSQGDVEVEFRDATPASISLTGEVTEVERTGTMDDVMERAAAFAAANPPHHP
                     GSLARFPVPVEHYGVAVGVPFPILAVDALGRRGLFAPPRMAVVSWDTCEPVGAREFED
                     FDPDNWPPRRLSDWPAPQAARLTHEQLQATIQRFSACWSRVIDAWFKDRDWRDDVVFA
                     DAREALRWRGVLDPEAMQGVYRQMNQRFDAWVQALASAR"
     gene            complement(10692..11735)
                     /locus_tag="VGR22_10165"
     CDS             complement(10692..11735)
                     /locus_tag="VGR22_10165"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV2128971.1"
                     /translation="MTTNEPTRDQRQTREQPDGLSWHQRARHAVTNPTRGSVIRVVLA
                     IVIVLASLVIDRQFLGWGLFATLAILVVPAGRARSFAASFGPYAAVWFIFTFLRSLAD
                     ETRWAQIINTRASAIERWIFNGELPSIQLQARFYDPHDLSWWDFYLTFIHWSYFVIPH
                     AVAVFLWWKRPSRFLQYLRAMTLTLALGLVLYFALPSNPPWMAPESVNTPGAPTVLRI
                     MEPIAMELGGGLYQAGYRVVGESNPIAAMPSIHMAITFLLVPASYYYGRVWQILAWIY
                     AFSMGLALVYLGEHYIIDVVIGSVIAGYSWRAARTWMLDVFPAIRRLLSFRSRAPESR
                     RVRPQRRPAGSNV"
     gene            complement(11790..12317)
                     /locus_tag="VGR22_10170"
     CDS             complement(11790..12317)
                     /locus_tag="VGR22_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEV2128972.1"
                     /translation="MYWTKRHVLVCTSQHCNQKGAMDVLGRLRLEIIRKRLDAEIFVN
                     NCGTIDLCDTGPNMVIYPDNIIYGGVTASDIPDIVTFLQGGPVVERLHLQAGKRFEGK
                     RRDFYAEIVERGIESEDEMREVARKHDLDEVMVDEQFRRGFMARKPVEGDEEAIRVHP
                     TKKALTRYGLLSKAT"
     gene            complement(12350..12679)
                     /locus_tag="VGR22_10175"
     CDS             complement(12350..12679)
                     /locus_tag="VGR22_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEV2128973.1"
                     /translation="MTVNIPLDTRNTILDPAAIERKLALNTYNVDSDRAHIRIIDHQV
                     CLSCVRQQCINCCPANCYVPQYDGTVLFSYEGCVECGTCRIVCNEFDNIEWTYPRGGF
                     GIQYRLG"
     gene            complement(12780..13914)
                     /gene="prfB"
                     /locus_tag="VGR22_10180"
                     /pseudo
     CDS             complement(12780..13914)
                     /gene="prfB"
                     /locus_tag="VGR22_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922581.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
BASE COUNT         2462 a         4359 c         4710 g         2540 t
ORIGIN      
        1 caggcgcacc aggatatcga aggtcaggaa ggtgaaggcg tgcccgatgt gagtggtgtc
       61 atacggcgtc acgccgcaga cgtagattcc gacgtgtccg tctctcggga cgaactcgtc
      121 aatcgagcgg ctctgtgagt tgtacagacg catacgcgcg gttgcccttt cgccgtcatc
      181 ggcttggtga agacacgtcg ccgcaccgac gtctgcgttg cggcacctcg atgttcggca
      241 gtatatcgca ggattttcgc tgtgctccgc ggccccggac agcgccacgg tggctgttgg
      301 cagggccgga gggtgtatga tcgagaggtc gtcactggga gcatccggaa tcgattcgct
      361 gatcttcatc actacggatt cgtcatggtc ttcctgttct cctggcgcac aacatcgatg
      421 atcctcgggc tgctgccact cgcgttcgtc ctggcgctgc cggtcacgga ggcgcaggca
      481 agccaagcca cacccccggc aacttcgacc tctgcgacgc cgacgccgat gccgaccgaa
      541 gagctgacga cgcccgagcc gccaatcacg ggagacccga acgcctgcgt cgacgggcgg
      601 cttcacatca gtgacctcgc caatgctgac ctgcacctgc aggaaggact ccatcgggta
      661 agtaagcgcg ccgaaatgtg gcaatccgac gcccagctag tcgagctccg gctggcgtgc
      721 cccctgctca agactggtct tcagtgggag ggtaccttct tctccccatc ggcccaggcc
      781 aacttttcca ctgacacctt tgatgtcgag ccgtccgagg aggcgccaga tgaggtgccg
      841 tatctcgatg tcgcgaacat ctcgttccag gcagtgcacc gctcgctgct gcgagccgga
      901 tattccgacg acctgcggct cgccgccgcc agcagcgtca ccatccgccg cagtaccgag
      961 cagacgccct tcgggccacc gatcgcaccg ccgggggtcg tctacgtgca tgtcgccgtg
     1021 gaggagcgcg gcgtcatcaa ggatgtctgg gtcaacgccg aagacggaac catttatcgc
     1081 tacgatcttg gagggtagag ccatggcgaa cagaccaatc gaggccgatg ttgcgagcgc
     1141 ggtcgaccat accgtcgcgg tactggcgaa gcgtcctgcc ggtctgctct tcgactatga
     1201 cggcacgctc agcgagcttg tcaacgaccc gacgctggcc gccattgagc ggcggtccga
     1261 ggcggcgatc cacgagttga cggatttggt cgacgtggtg gcgatcgtca cggggcgtgc
     1321 ggcgagggac gttcagacgc gactcgacac gagtgacctg gtggtggtcg gcaaccacgg
     1381 gtttgagtgg gtggtgcagg acgtccacac ggagcatgat gctggcgtcg acgctgccaa
     1441 cgccgtcgag aacgcgctgg ccgagatcga ggagcggcta acgcgggaca atctcgtcga
     1501 gggcgtgatc ttcgagaaca agcgtctctc cggcagcatt cactatcggc aggcgcccaa
     1561 ccagcggaag gtcggcagcg tgctcattcc cctcgcgcgg aagatcgccg cagcgcacaa
     1621 cctgcggccg accgagggca agatgatcgt ggagctgcgc ccgattgcag aggtcaacaa
     1681 gggcaccgcg atgtaccaca tccgggatga gttcaacctc gccggcatgg ctttcattgg
     1741 cgacgacctg acggatgtcg atgggtttga atcgctgcgc gacattcgca acgccggggt
     1801 ggctgacact gtcgcggtgg gtgtgctcgg cccggacacc catccgagca tcgcgctgac
     1861 cgccgatgtc gccgtaacgg gtgttctggg tgttgccgac tatctcgagc ggctcgttgc
     1921 gcgcctccgg gaggttgttt cctgatggag agggggcagt aggcggagaa atgaccgcag
     1981 gaggtacccg accggggccg ggacggagga ggctgcctct ggcacggggg tgatcatctc
     2041 ctcagccggg attcagtctg cctgagcatc cgctcgaggc gatcctgccg gaggtcgaag
     2101 gtgatgtcgc cgcgggttcg ttccagcacg gcccggagct ggtcggttgt acggcggata
     2161 ccgccggtga ttgcgtctgg gaagacgacc gtcaccaggg agctgtagat ggtgtccatc
     2221 tgttgcagca gtgactccgc ccgcggcaca tcgctgtagc gaagcgcgtc gagcgcgtcc
     2281 cgccgaagct cactggcggc ctctgccagc ccgttcagcc atgccgacga cgagacaccg
     2341 agatcgccgg ccgccggcag gtcactgccg ctcagaatgg cgatgctgat atgggcctcg
     2401 gcgacctcct tcatcgcgtc ctgcacgtat ccagcgccgg ccggggacgg gcgatcggtt
     2461 acatggtcga cgatctcctg cagtatcccc gaggcctccc cggcaccgga ccgggcgtca
     2521 tcgaactcgc cgcgatgagc ggcacggatc gcatccgcgg acgcgcgaat gacatgccgg
     2581 ctctgcgaga tgggcttctc gcgcgcggcg ttgatcgact cgaactcgtc gataatacct
     2641 gcgaccagca gggccgtgtc gttcgtactc atggatgctc ctccgcgtgt ggcaccgtgc
     2701 tcggtgccgc gttgacactg gagagtctag ccccgtatac tctcaactgc tgccgcgcga
     2761 tacggcggcg cggccggtat ggccgaaaca taacatggcg atgatcggaa gcagtaggcg
     2821 cggctggccg agagagatgt cggtgctgcg agacatcgac cgagcgctga actcgtccgt
     2881 gagccggaac cgcgaaagca gtagtggttc ccgggagctc ccgatacagg gcaatgagcg
     2941 ggcttcgaga aacggcccga acgcgacaat ccagacgatc atggggccgt agctcagctg
     3001 ggagagcgca acactggcag tgttgaggtc aggggttcga gccccctcgg ctccaccaat
     3061 ctggtccgcg tctacagccg tcgacgaagt caaagtgggt ggtaccgcgg atggcaagcg
     3121 cctttcgtcc caacacgggg cgggggcgtt ttttcgtatt ccgtttcgaa caggagcagc
     3181 atgatggcga ccacgacgac agatacccgg cgcgacatcg agcgtacctg gaacccggcc
     3241 gagatcgagc cgaaatggcg cgagcggtgg caggaagcgg cgatctatca ggtcgacgac
     3301 aacgcgccgg aaccgaagtg gtactcgctg acgatgtacc cgtatccgtc ggggacgctc
     3361 cacgttggtc actggtacgc gtttgtggga ccggacattt acgctcgcgc caagcgcatg
     3421 cagggctaca aggtgctgtt ccccatgggg tttgacgcct tcggccttcc cgccgagaat
     3481 gcggcgatcc ggcacaacat ccacccagcc acgtggacct acgagaatat cgagcagatg
     3541 atcgcccagt tcgatctgat ggggccgatg atcgactggt cgcgcaagct gatcacgtcc
     3601 gaccccgagt actatcgctg gaaccagtgg attttccttc agatgctgga gcgggggctc
     3661 gcctaccggg ccgctggctc ggtctggtgg tgcccgaatg accaaaccgt gctggccaac
     3721 gagcaggtgg tcgagggcaa catctgcgag cgttgtggtg ccgaggtcta caagcgtgac
     3781 ctggagcagt ggtacttccg aatcaccgag tatgcggagg agctgctggc cgagctggac
     3841 aaccttgatt ggcccgagcc ggtcaaggtc atgcagcgga actggatcgg tcgtagcgag
     3901 ggcgcccgcc tgtccttccg gttggagacc ggcaacgagc tggaggtctt cacgacccgg
     3961 cccgacaccg tcttcggcgc aaccttcatg gtgctggctc ccgagcaccc gctggtcgac
     4021 aagatcacca ccgatgagca tcgtgaggct gtcggcgcgt accgcggtga ggctcgccgc
     4081 aagtcagaga tcgagcgcca gtcgaccgac gaggcgaagg agaagaccgg cgtcttcacc
     4141 ggagcctccg cggtcaatcc ggtcacggat gagcggattc cggtctggat tgccgactac
     4201 gtgctgatga gctatggaac gggcgcgatc atggcagttc cggcgcatga cgagcgcgac
     4261 ttcgccttcg cgcgggcctt cgatctgccg atccgcgtcg tgatccagcc ggagggggag
     4321 gacgctcttg acccgcgcac catggaggcc ccctacacgg gcgccggagt gatggtcaat
     4381 tcgggacagt tcgacggcac cccgacgccc gagcagctgc cgaaggtcat cgcctggctg
     4441 aatgagcagg gtcgcgggca ggcggaggtg acctggcggc tgcgggactg gctgatatcc
     4501 cgtcaacgtt actggggcac gccgtttcca atcgtctact gcgacgactg cggcatggtt
     4561 cccgtgcccg aggaccagtt gccggttgag ctgccgctgg acgccgagtt cacacccact
     4621 ggacagagcc ccctagtgaa ccacgaacgg ttcctcaaca ccacgtgccc atcctgtgcg
     4681 ggaaccgctc ggcgtgaaac cgacacgatg gacacgttcg tcgactcctc gtggtactgg
     4741 ttccgctata ccaacccgaa cttcgatgac gcaccgatcg atgaggagcg aacgaagctg
     4801 tggacgccgg tggatatcta caccggcggc atcgagcacg ccatcctgca tctgctctac
     4861 gcccgcttct tcgccaaggt cctgcgcgac ctggagctgg tcgatcacgg ggagcccttc
     4921 aagactctgc gcaaccaggg catcatcctg gcggcggaag ggaccaagat gagcaagagc
     4981 cggggcacgc aggttggccc ggatgagctg gtggccgccc acggcgccga tgcgctgcgg
     5041 ctgcacctga tgttcctggg tccatgggag caggggggac cgtggaacga gcgcgggatc
     5101 accggcatgg agcggttcat ccggcgcgcg tttcaggtgg tcacggaaac tgccggcaac
     5161 cggatggacg tgaaggccga cgcggcagac ctgcggcgcc tgacgcacaa gacgatcgca
     5221 cgcgttaccc gggatctcgg acagttccag ttcaacacgg cgatcgcagc gctgatcgag
     5281 atgacgaacg agctgatgaa gctcaaggag accgaggtcg ccgagacgac ggcatggcgg
     5341 gacgccatca ccgcgctgac gctgctgatg gcgccgctaa cgccatacac ggccgaggag
     5401 atgtgggaga tgctcggcaa cgagttctcc gtgcatcagc aggactggcc ggtctacgac
     5461 gagtcgctgg cggcggtgga gacggtcgag attcccgtgc aggtcaacgg caaggtgcgc
     5521 gagcgggtgg cggtcgcggt cgacgcgagc gaggaagatg tcacagcggt tgctcgcgaa
     5581 accggcaaga tcccggacta cctgcagggc aagaccctga agcgcacgat ctacgtgccg
     5641 ggtcggctga tcaacttctt ggtcgggtag gcgaggatcg ccacgtgagt tctctaccgt
     5701 cacaccgagc ctcgggcggc gaagtcgcgc caacttcgca cggagattct ccgctgcgct
     5761 cagaatggcg attggtcggt gtcgtcgcgt ctcgttgctg aatgtggtgg attcagggcg
     5821 aaccggagtg aagtcgctgc tcccgcgcag cgcgcgagac ggtacaagaa cacccccgga
     5881 ttgtgcgatc cgggggtgtt cgttttgtgt ctcatctgag cggtgctagt ccgcagcgac
     5941 agcttccttc tggcgctccg ggatccagcg gagattcagg tttcggttcg cgccgacctc
     6001 gtcgagccgc ttgtactccg tcgtgtgcgg cgcgtgcttg accttctccg ggtcgctctc
     6061 cgcctcctcc gcgatctgca gcatcgcgtc gacgaagtag tcgagggact ctcgcgactc
     6121 ggtctccgtc ggctcgatca tcagtgcctc ggggacgatc agcgggaagt aggtggtcgg
     6181 cgggtggacg ccgaagtcca acaggcgctt ggcaatgtcc accgtacgga cgccgttttt
     6241 cttctggtgc gatgccgtgg ccacgaactc gtgcatgcag gtgcggtcgt acggcacgtg
     6301 gtaggcctcg tgcagccggg cgcgcagata gttcgcattc agtaccgcgt cgacgcttac
     6361 ctgccgcagt ccgtcctcac cgtgcattcg gatgtaggtg taggcgcgga tgtgcatgcc
     6421 gaagttgccg ttgaaggcat ggagcttgcc gatgctgcgc tccggttgct tccaggcgaa
     6481 acgcggtccg tcctcctcgt cgacgcgggt gacgatcggc cccgggaggt actccgcgag
     6541 ctccttcgtc acgccgaccg ggcctgatcc cggaccgccg ccgccatgcg gtgtcgagaa
     6601 ggtcttgtgc aggttgaagt gcatcacgtc gatgcccatg tcgcccgggc tcgcgatgcc
     6661 gaggatcgcg ttgaaattgg cgccgtcgtt gtagacgagc ccacccgcct cgtggacgat
     6721 ctggatcatc tcctggatgt gctcgtccca gagtccgagc gtgttcgggt tggtgatcat
     6781 cagcccggcg gtgtccggtc cgaccagctc acgtactgcg tcgatgtcgg tattgccccg
     6841 gttgtccgac ggcacggtga caacgtcgaa cccggccatg cgtgccgtcg ccgggttggt
     6901 gccgtgcgcc gagtccggaa tgagcatctt ggtgcggttt tcgccgccgt ggtccttgtg
     6961 ccacgccttg atcatcaggc agccggtgag ttcgccgagg gcccccgcgg ccggctgcag
     7021 cgtgaccgcc tcgaagcccg atatctccgc gaggatgttc tggagctcgt gcatgagctc
     7081 cagcgctccc tgaacggttt cctccggcgc cttcggatgg atgtttgcga agcccggcag
     7141 ccgtgcgacc ctctcgttga ccttcgggtt gtacttcatt gtgcacgacc cgagggggta
     7201 catattgatg tcgatggcgt ggttcttctg gctgaccctc gtgaagtgcc gtaccacgtc
     7261 gatctcgctg acctcgggcc agttgagctc gttccgccgc aacgatgcgg gaatttccgt
     7321 ctccggcacg tcgggttccg ggatccggac cgctcgacga ccggggctgc tgagctcgaa
     7381 aatcagggat tctaccgcca ttcagacaga ctccgtgggg gtgcgcctcc ggggccattt
     7441 gtggctctgt ggcgacgatt agcatgtcat cgtggatggt agcacggtga cagagcacgg
     7501 caacatgcag acgcgacgct gcgatcacgt cttgctgggg tgcctgactc cgacgaaatg
     7561 accgcgtcga cggtcacccc gacggagaca cggccccggt ggcgctccca gatcgatggt
     7621 ggtgggctcg gcccgatgtc ctcgcgatca tgggagcgat tcttcgcctt gcttgcgatg
     7681 aggcattcgc cgagaggagg ggggtggaac gcgcagcgtc acgtcaccaa ctacatgcta
     7741 cacgggtatt gcggtggtat ccgccgatgt atggtcgctg cgcctccact gcatccagcc
     7801 catcacgact gccatgatcc cgcccagcac cgctcccgtg aaaatgggca cgctcggcga
     7861 gatgccgtag agctgcgatg cgatgatcgg tccgaacgac atcgcactcc cgccgagcat
     7921 ctccaacacc gcgaatgcgc gtgagcggag ccgtgtcggc gcgatattcc ccatctcggc
     7981 gaggatcatc gcccaggtcg cgaacatgcc gccccgcagg aagaacgcca gcgcgatgag
     8041 cggtagcgcc tcggcgaacg tgaagacgaa gagtccggcc acgattgccg cgcaggagat
     8101 ggtcgcggcg cgcatcgggg agttcgagag tgtcggcgtt cgcgccaccg caagcccgaa
     8161 gagagccgtg ccgagggccg agccggcgcc gagggtggcg atcagcgccg gatcaagccc
     8221 gcgggtgtcg cgcaggaaga cggggatcag cgagatacca atggccaggg agaggatcgt
     8281 cacgccatgg acggcgaaga gcgggcggat cgagctgttg gcgaaggtgt ccccatagtt
     8341 tggttgagca gcctcgccgt cgtcctcgtc tctggggaga gagaggtcca tccggctcag
     8401 aatgaggatc gatgcgagcc ctgtgatggc acccagcacg aaggccgacc gcatgcccca
     8461 ctgcgcgata agtgcgccgg agaggagtgg ggtgatcatg agggccacgg acggcccgac
     8521 attgatgatc atcgagaagg ctcgcgcacg gtcttcgtgt gcccgttcgg cgacataggt
     8581 ttgcaggagc ggaaagacca tctcgccgat cgcgttcagc accacagtga tgaagaggat
     8641 cggccatgcc tgagcgaggg cggcaacgac catgccggcg atgcccgaga tgcgggcggc
     8701 gatcatgagg ggcttccggt cgacccgctc cgtcagccag gacgaggggc cgatgaccag
     8761 tgggcggatg aaccctgcgg agccgagcac gagacctacc agcgcgatcg gtgcaccgag
     8821 ttcctcgatc cacagcggcc acacgcccat gtaggcgccg aacgtcgcct cgaagaacgt
     8881 catcgcccag aaggcaagtc ccaggtctcg accgagaccc acatcccaac gcagtcgcat
     8941 tccagtcctc agcacatggg gtcgcggcgg ccatcattcg agcccgagcc attgtagccg
     9001 gtggaatgaa ggtaggttag cggaagcgcc gggccctgct cggcaggggc agtacaatgg
     9061 gtcgtggcgt gcgctcgcgc gtcttcccga ccctaccgcg tggaacggtg gtgctccctg
     9121 tggatgccta tctgctgacc ggcctcgcca tgtgcgctgt actgctcgtc gcgctagcgg
     9181 gcacggccta cctggccgtc tttttcaatc ggcgcgccaa ggacgatctc acggctgcct
     9241 tcacgccgct tgccaagcgc atggatggct cgttcgagct cgacaacgcc gagatcaggg
     9301 gggcgtacca gaactatccg gtcttcgccc gcatggcgaa cgcgaccgag gggcccggcc
     9361 gcgtttttca ggtcgacgtg ctcgatgccg ccggtggtga tctgtggcag tacacctcga
     9421 atccgccgac gaagcgggag ccggagcgga cggtcgagtt cgagggcgac gacgagatcc
     9481 ggcagatcgt gaagccagcg atcgaaaccg agtcgcgccg ctttctcgat cccgacattg
     9541 agcggtttcg ggtggagtac cttccgaggg agggggtcgt gcggctcgtc cgggcgatgc
     9601 ggtcccggcg ggacatcccc gatgccggca cgctagcctc cgagctcgac atgctgaccg
     9661 gtatcgccgg gcgcaaccgc gcgtgggtcg agtcccggaa cggcggggag gtgcgggcgt
     9721 gacgcgcgct atcgtctacg tcgattccgc tgaggagcag gtcgcgtacc tcatcgctcg
     9781 gttcggccca caaggcgcct ggtcggtgcg ggagcagcgc gtcgccacgc tcgacaacgg
     9841 cgcctcggtc gagcgagcgt ccctgcagac gtcgcagggc gatgttgagg tcgagttccg
     9901 ggatgcgacg ccggccagca tctcacttac cggtgaggtc accgaggtcg agcggacggg
     9961 caccatggac gatgtgatgg agcgagctgc ggccttcgct gctgcgaacc cgccgcatca
    10021 ccccggcagt ctcgcgcgct ttccagtgcc cgtggagcac tatggagtgg cggttggggt
    10081 gccgtttccg atcctcgcgg tcgacgctct cggtcgtcgg ggactcttcg cgccgccgcg
    10141 catggcagtg gtttcctggg atacctgcga gcctgtcggt gcccgcgagt tcgaagattt
    10201 cgatccggac aactggccgc cgcggcggct cagcgattgg ccggcgccgc aggcggctcg
    10261 tctcacccat gagcagctgc aggcgacgat ccagcggttc agcgcatgct ggtcgcgggt
    10321 gatcgatgcc tggttcaagg accgagactg gcgggatgac gtggttttcg cggacgctcg
    10381 cgaggcgctg cggtggcggg gagtgctcga tcccgaggcg atgcaggggg tctaccgcca
    10441 gatgaaccag cgcttcgacg catgggttca ggcgctggca agcgcccggt aaccgcatca
    10501 cgcagcgttc gatggttcgg gccactgcct acacaccctt ccgcctcgcg gtaacaccgg
    10561 cccgaccccc gatcagtcgc gcctacaggt tcctgtcgac gttgagacag ccgatttgat
    10621 ttcaccctgt ctctctatcg tcgtgactcc tgacgtgcaa ggggaggcca tcaggtcgat
    10681 cgcgggcgga gtcagacatt cgagccggcc gggcggcgtt gcggacggac gcggcgggac
    10741 tccggcgcac gcgatcggaa cgacagcaac cggcggatgg ctgggaagac atccagcatc
    10801 caggtgcgag cggcgcgcca gctgtacccg gcaatcacac tgccgatcac cacgtcaatg
    10861 atgtagtgct caccaagata gaccagcgcg agtcccatcg agaaggcata gatccacgct
    10921 agaatctgcc agacgcggcc gtagtagtac gaggccggca cgagcaggaa cgtgattgcc
    10981 atgtggatcg agggcatcgc tgcgatgggg ttggactcgc cgaccaccct gtaccccgcc
    11041 tggtagagcc cgccgccgag ctccatcgcg atcggttcca tgatgcggag cacggtcggg
    11101 gcaccaggcg tgttgacgga ctccggtgcc atccatggcg ggttcgaggg cagtgcgaag
    11161 tagagcacca acccgagtgc cagcgtgagc gtcattgccc tcagatactg caggaaacgg
    11221 gacgggcgct tccaccagag aaacaccgct acggcatgcg ggatgacgaa gtacgaccag
    11281 tgaatgaagg tgagatagaa gtcccaccag ctcaggtcgt gcgggtcgta gaaccgcgcc
    11341 tgcagttgga tcgagggcag ctcgccattg aaaatccagc gctcaatcgc gctggccctg
    11401 gtgttgatga tctgcgccca gcgcgtttcg tcggccagcg atcgcaggaa agtgaagatg
    11461 aaccagacgg cggcgtatgg accgaacgag gcagcgaacg accgtgcgcg acctgcggga
    11521 acgacgagga tcgccagcgt tgcgaagaga ccccagccga ggaactgccg gtcgatgacc
    11581 agcgacgcaa gcacgatgac gatcgccagg acgacgcgga tcaccgagcc gcgtgtcggg
    11641 ttggtgaccg cgtggcgagc ccgctggtgc cagctcaggc cgtcgggttg ctcccgcgtc
    11701 tggcgttgat ccctggtcgg ctcgttggtt gtcaccttac gccctttcaa cgggagtcgg
    11761 ccgatcgata ctgcttgcgc aggcgaggat caggttgctt tggacaacag accgtagcgg
    11821 gtcaatgcct tcttcgtcgg atgaacgcga atggcctcct cgtcgccctc caccggcttc
    11881 cgagccatga acccacgccg gaactgctca tccaccatca cctcgtcgag gtcgtgcttc
    11941 cgcgcgacct cgcgcatctc gtcctccgac tcaatgccgc gctcgacgat ctcggcgtag
    12001 aagtcgcgcc gcttgccctc gaaacgcttt ccggcctgaa ggtgcaggcg ctcgacgacc
    12061 ggtccaccct gcaggaacgt gacgatgtcc gggatgtcgc tggcggtgac accgccatag
    12121 atgatgttgt cggggtagat caccatgttc gggccggtgt cgcagagatc gatggtgcca
    12181 cagttgttga cgaagatctc ggcgtcgagt ctcttgcgga tgatctcgag gcggagccgc
    12241 ccgaggacat ccatggcgcc cttctggttg cagtgctggg atgtgcagac cagcacgtgt
    12301 cgcttggtcc agtacatgtc atgttgcctt tcgccggcgc aggcccaggc taaccgaggc
    12361 ggtactggat accgaagccg ccgcgcgggt aggtccactc gatgttgtcg aactcattgc
    12421 agacgatccg acaggtgccg cactcgacgc acccctcgta ggagaagagc accgtgccgt
    12481 catactgcgg cacgtagcag ttcgccgggc agcagttgat gcactgctgg cgcacgcagg
    12541 agaggcacac ctggtggtcg ataatgcgga tgtgggcacg gtcggagtcg acgttgtagg
    12601 tgttgagcgc cagcttccgc tcgatagcgg cgggatcgag gatcgtgttg cgtgtgtcca
    12661 gcgggatgtt gacggtcatg acgcatctcc tggcacgatc accgctcccc gccgggtgcg
    12721 gtgatcggct cgtctgtttc ccggcttgtg ccgaaaagtc tagcacctgc ccggtcgagt
    12781 caggcggttg cgctggcttc ggtcgcgctc tcgtccgggc tgattagcat cttcatatag
    12841 ccctcgatga gcgggtcgat cgcgccgttg agcacggcgt tggcgtcgcc gatggtgacg
    12901 ccggtacggt gatccgtcac ctgtgtgtat gggtgcagca cgtatgaccg aatccggctg
    12961 ccgaagccgg cggccgtgag gctgcccttg aggcgatttc gctcctccgc ctgctcttcg
    13021 agtttccgct ccagcaaccg agcgcgcagg atcttgaagg ccgcatcgcg gttctgctgc
    13081 tggctgcgct cgttctggca cgagaccacg atgctcgtcg gcaaatgcgt gagccggacc
    13141 gcagagtccg tcttgttgac gtgctgtccg ccggcgcctg aagagcggaa ggtgtcaacg
    13201 cggacgtcgt ccggccgcag gtcgacctcg ctgtcgctct cgatctccgg gatcacctcg
    13261 accagcgcga acgccgtatg tcgacggtgt gcggaatcga agggggagag ccgtaccagt
    13321 cggtgtggtc cgccctcgcc cttgagaagg ccataggcgt gcgaaccgcg aaactcgatc
    13381 gtcgcgttct tgacgccggc ctcctcaccc tcggtcgtgt cgagcagctc ggccttcagt
    13441 tcgttacgat ccgcccagcg caggtacatg cggctgagca tctctgcgaa gtcctgcgca
    13501 tcgacgccgc cctcacctga gtggacgatc atgatcgcgt tgttgtcgtc gtacggaccg
    13561 ctcagagcca gttgcagctc caggtcatca acacgctccg tcaaggcatc gcgctcggct
    13621 gagatctcgt cgaggaactc gtcctcgccg gcggccatct cgaccatctc gcgcagatca
    13681 ctgctggcgg tgcgcaactc attccactgg ctgacccggg ccttgagctc accgaggcgg
    13741 cgcatctcgc gctgggcggt gtcggggtcg tcccaatagc cttccgcgcc gctgcggcgc
    13801 tcgaggtcgt ctatctctcg ctcatcgctg gcgaggtcaa agacgcaccc cgagttcctc
    13861 aacgagctcg ttgatctcgg caagactgcg ttgcagttcg tctcgtgatg ccatactggc
    13921 tgtctcctga aacagagttc gggtggtgtc ggtcagcttg gcgatgaacg ccacggtgga
    13981 gtatacgatg ctgctgtagc ggggcctgtc gccggttggc gctggggcga caccctctgc
    14041 gacactggcc ggagcgatac caggattgtt g
//
LOCUS       DASYWL010000288         5975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_446644, whole genome shotgun sequence.
ACCESSION   DASYWL010000288 DASYWL010000000
VERSION     DASYWL010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5975
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446644"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VGR22_10185"
     CDS             complement(<1..234)
                     /locus_tag="VGR22_10185"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2128974.1"
                     /translation="MLDEPTSGLDPLVRDTFLDILLGARQRGQTVFFSSHNLAEIERV
                     ADRVGIIREGEMVAVETPRDLTARAFRHGRISFA"
     gene            complement(237..626)
                     /locus_tag="VGR22_10190"
     CDS             complement(237..626)
                     /locus_tag="VGR22_10190"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2128975.1"
                     /translation="MVDLDVWEGEIFGFLGPNGAGKSTTMRTLLGFMAPTSGTGRIFG
                     LDIVDDSVAIRQHIGNLPDTFAMEDRLTGRQLVELFARLRGVTDLSQMEALARRLDAD
                     LDRPTRKLSSGNKRKIGIITALFHQPA"
     gene            1203..2231
                     /locus_tag="VGR22_10195"
     CDS             1203..2231
                     /locus_tag="VGR22_10195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2128976.1"
                     /translation="MRAVDDVTFEVADGELCVLIGPSGSGKTTTMRMINRLESISRGT
                     ISIDGKDVMPMNVAELRRNIGYVIQQGGLFPHFTVAENVSVVPKLLGWSKQRRRERAE
                     DLLELVGLNPRLHADRYPSQLSGGQQQRVGVARALAADADIILMDEPFGAVDPITRNQ
                     LQRWLRQIQAEVHKTIVFVTHDINEAFLLGDRIVLMNNGAIEQNGTPEELLREPASDF
                     VREFIGDEFALQALEHTYIEELARTGGPRPPKNALILPGKLSLAEASRALREAEGVGG
                     EGFWVATEEGEPEGYVTYHEFAAMLGEGVASATMVSDENVDGDAGEIIADRSEAHGKE
                     DAGAVHPR"
     gene            2212..2850
                     /locus_tag="VGR22_10200"
     CDS             2212..2850
                     /locus_tag="VGR22_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2128977.1"
                     /translation="MQYILDNPEIVWQRTLEHFHLVGIAVLMATVVAVPLGILISKVR
                     WLAIPVLNLTGVLYTVPSLAFFAILIPYTGIGRSTAIYALAIYSLLAIVRNTAAGMDE
                     VPNATIDAARGMGMTSWQILRMVQLPLALPYILTGIRIASVAAIGIAAIAAVIGAGGL
                     GRLIFDGIRRLDSDLIVAGSIMAILLALLVDFGLSRLALLARRDLRAPAQGG"
     gene            2857..3519
                     /locus_tag="VGR22_10205"
     CDS             2857..3519
                     /locus_tag="VGR22_10205"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2128978.1"
                     /translation="MFGSNDPLDFIDWIRDNPDQFWELLREHAELVFYAVGIALVIAV
                     PLAVVTSRIKVLERPMTWVANIGQAIPNLAVLGLVLPWLGIGFRPSLFALTILAILPI
                     FLNTLVGIVSVDPAVVDAARGMGTTDFQLLRMVQLPLAMPFMFAGLQTAVVQSIGLAT
                     LAAYIGGGGFGDWILQGLAMNSYPLLLAGAVPVAVMAMSAEILLGLARRTVVPKGLTV
                     AD"
     gene            3629..4633
                     /locus_tag="VGR22_10210"
     CDS             3629..4633
                     /locus_tag="VGR22_10210"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV2128979.1"
                     /translation="MGRFIRTISVVLIAFGLIVGSLTGATGATAQDEDKPTVTIGSKD
                     FTESILLAEMAAALLEDNGYTVERQLNLGGTAVVHQALVNGEIDMYVEYTGTGLLAIL
                     GRDLPETDATPEAGAAPEEGATPAVSEEAAYTGCLGAVYDIVAEEYPEQFQVEWLQPW
                     GFNNTYALAVTQETAEEYGLETVSDLEGVAGELTFGATQEFLVRPDGLPGLEEHYGID
                     FGDERGLDPGLLYEAVDQGQVDVISAFATDGRIPALDLVVLEDDQNFFPPYCAAPVVR
                     QDLLEESPEVRDILNQLAGQIDDETMADLNFQIDEGGTEPSEVAQNFLEEQGLIGGDS
                     "
     gene            4898..5413
                     /locus_tag="VGR22_10215"
     CDS             4898..5413
                     /locus_tag="VGR22_10215"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEV2128980.1"
                     /translation="MSQQQNIDLVRLSESNLTLGDENEDIRGRKVIDRAGDEIGEVED
                     LMIGREDARVFFLLVGSGGFLGIGEKTFAIPVDAITRIDEQHVHVDRTREHVVGGPEY
                     DPSIVREGSASGSSNSFWEDTYAHYDYGPFWAPGYTYPAYPYYETGGPGRPDAPGVPD
                     DYGTSGRRTGV"
BASE COUNT         1017 a         1856 c         2031 g         1071 t
ORIGIN      
        1 cgcgaagctg atgcgcccgt gccggaaggc gcgcgccgtg aggtcacgag gcgtctcgac
       61 ggcgaccatc tcaccctctc ggatgatgcc gacgcggtcg gcaacccgct cgatctcggc
      121 caggttatgt gaggagaaga agaccgtctg tccccgctgt ctcgctccga gcaggatgtc
      181 gaggaaggtg tcccgcacga gcgggtcgag gccgctggtc ggctcgtcca gcatgatcag
      241 gcgggttggt ggaagagtgc ggtgatgatg ccgatcttcc gcttgttgcc acttgagagc
      301 ttgcgcgtcg ggcggtcgag gtcggcgtcg agccgccgcg cgagcgcctc catctgcgag
      361 aggtcggtca cgccgcgaag gcgggcgaag agttcgacca gctgtcggcc ggtgaggcgg
      421 tcctccatgg cgaacgtgtc cggcagattg ccgatgtgct ggcggatcgc gacgctgtcg
      481 tcgacgatat ccaggccaaa gatccgcccc gtgccgctgg tcggcgccat gaagccgagc
      541 agggtgcgca tggtggttga cttgccggcc ccgtttgggc cgaggaagcc gaaaatctcg
      601 ccctcccaga cgtcgaggtc gaccacgatg gcggggtggt catgtagtct ccgattcgtc
      661 cgacgtccat caggacctga ggaagtgatc aacggcagtg ccctgtagcc ctgcgtgacg
      721 ttggctgcga cacgctgccg acggacggcg aattggtcga tagccgttgt tgcccgctgc
      781 cgagttccgc gagcgcctcc cggccgtcat cggcacgtcg cactcatgat cgcctgctcg
      841 tgacgtccgt tcaacggtga gacatggccc ttccgggggt gcggaacggt gttccttgtg
      901 aggcaatcat aatcggtgga aatgtccggg ggcacgggat gtgcagcacc gccggggagt
      961 cgaacccgcc ccgcgcgcag ttggtcctgg ccgggtgccg tgccgaggtg ttctacactg
     1021 atcccctgag cgtctggaat ctgcatggtc ggtggtgcat cgctggcatg cagggacaag
     1081 actgcgcgct cacccaagac accacgtcgc catgtgggtg agcgggagcg agctcccaag
     1141 ggaggacacc gttgatcaca ttggatcatg tgtcgaaaat tttcgggtca ggggatcagg
     1201 aggtgcgcgc cgttgacgat gtcaccttcg aggtcgctga cggcgagttg tgcgtcctca
     1261 tcggaccgtc gggttcgggc aagacgacaa cgatgcggat gatcaatcgc ctggagagca
     1321 tctccagggg cacgatcagc atcgacggca aggacgtcat gcccatgaac gtcgccgaac
     1381 tgcgccgcaa catcgggtac gtcattcagc agggcggcct gttcccacat ttcacggtcg
     1441 cggagaacgt ctcggtcgtg ccgaagttgt tggggtggtc caagcagcgc cggcgggagc
     1501 gcgccgagga tctgctggag ctcgtcggtc tcaacccacg gctccacgcc gaccgctacc
     1561 caagccagct ctcgggtggc cagcagcaac gcgtcggtgt ggcgcgcgcg ctggcggccg
     1621 acgccgacat catcctgatg gacgagccgt tcggcgcggt ggaccccatc acacgcaacc
     1681 agctgcagcg ctggctgcgc caaatccagg cggaggtcca caagaccatc gtcttcgtga
     1741 cccatgacat caatgaggca ttcctgcttg gcgaccgcat cgtgctgatg aacaatgggg
     1801 cgatcgagca aaacgggact cccgaggagc tgctgcgcga gcctgccagc gatttcgtac
     1861 gcgagttcat cggtgatgag tttgccctgc aggcgctcga gcacacctac atcgaagagc
     1921 tcgcccggac gggcgggccg cgcccgccga agaacgcgct gatcctgccg ggcaagcttt
     1981 ctctggccga ggcgagccgt gcgctccggg aggccgaggg ggtcggcggt gaggggttct
     2041 gggttgccac cgaggagggt gagccggagg gctacgtcac ctaccacgag ttcgccgcca
     2101 tgctcggtga gggtgttgcc tcggcgacga tggtgagtga cgagaacgtc gacggtgacg
     2161 ctggcgagat catcgccgac aggtcggagg cgcacggcaa ggaggatgcc ggtgcagtac
     2221 atcctcgata atccggagat cgtctggcag cgcacgctcg agcacttcca cctcgtcggc
     2281 atcgcggtcc tgatggcgac cgtcgtcgcc gtgccgctgg gcattctcat ttcgaaggtg
     2341 cgctggctgg cgatcccggt gctcaacctc accggcgtgc tctacaccgt gccgagtctc
     2401 gccttcttcg ccatcctgat tccctacacc gggatcgggc ggtcgacggc aatctacgcg
     2461 ctggcgattt actcactgct ggcgatcgtg cgcaacacgg ccgccggcat ggacgaggtc
     2521 cccaatgcga cgatcgacgc tgcgcggggc atgggcatga cgagttggca gattctgcgc
     2581 atggtgcagc tgccgctcgc gctgccctac atcctcacgg gcatccgcat cgcctccgtg
     2641 gcggccatcg gtatcgctgc aatcgcggcg gtcatcggtg cgggggggtt ggggcgcctg
     2701 atcttcgatg gtattcgccg gctcgacagc gacctcatcg tggcagggtc catcatggcc
     2761 atcctgctgg cgctgctggt cgacttcggg ctgtcgcgcc tcgcgctgct ggcacggcgt
     2821 gatctgcgcg cgccggcgca gggagggtag acctggatgt tcggaagcaa cgacccgctc
     2881 gatttcatcg actggattcg cgacaaccct gatcagttct gggagctctt gcgcgagcat
     2941 gccgaattgg tgttctacgc ggtgggaatc gccctcgtca tcgcggtgcc gctggcggtg
     3001 gtcacgagcc gcatcaaggt cctcgagcgt ccgatgacgt gggtggccaa tatcggacag
     3061 gccattccga acctggcggt gctcggcctc gtgctgccgt ggctgggcat cggcttccgc
     3121 ccgtcgctct tcgccctgac catcctggcg atcctgccga tcttcctgaa cacgctcgtc
     3181 ggcatcgtca gtgtcgatcc cgcggtggtc gatgcggcca gggggatggg cacgaccgac
     3241 ttccagctgc tgcggatggt gcagctgccg ctcgcgatgc cgttcatgtt cgccgggctg
     3301 cagactgcgg tggtccagag cattggcttg gccacgctgg ccgcctacat cggtggcggg
     3361 ggcttcggtg actggattct gcaggggctg gcgatgaaca gctacccgct gctgctggcc
     3421 ggtgcggtgc cggtcgcggt catggcgatg tcggccgaga ttctgctcgg gctggcacgc
     3481 cgcacggtcg tgccgaaagg gctgacggtc gccgactaac catggccagg cagggggggc
     3541 gccctgggca ggttgcaccg atgcggtaag gttaggtggt gcacatcgac agacgcgaca
     3601 tgtgtcgaat caggaaagga gcgttccgat gggacgattc atccggacaa tatccgtggt
     3661 cttgatcgcc ttcgggctca ttgtgggctc gctgacggga gccacgggag ctaccgcgca
     3721 ggacgaggac aagccgaccg tcacgatcgg ctcaaaggac ttcaccgagt cgatcctcct
     3781 cgccgagatg gctgcggcgc tgctggaaga caacggctac accgttgagc gtcagctcaa
     3841 cctcggtggt accgccgtcg tccatcaggc cttggtcaat ggcgagatcg acatgtacgt
     3901 cgagtacacc gggacagggc tgctggcaat tctcggccga gacttgcctg aaacagatgc
     3961 cactccggag gctggtgcag caccagagga gggcgcgacg ccagcggtga gcgaagaggc
     4021 agcttacaca ggctgtctgg gcgcggtcta cgacattgtt gcagaggagt acccggagca
     4081 gttccaggtc gagtggttgc agccgtgggg gttcaacaac acctacgcgc tcgccgtgac
     4141 gcaggagact gccgaggagt atggccttga gacggtctcc gaccttgaag gcgtcgctgg
     4201 cgagctgacc tttggcgcga cgcaggagtt cctagtgcgg ccggatggcc tgccgggcct
     4261 cgaagagcac tacggcatcg acttcggtga cgagcgggga ctcgatcccg gactgctcta
     4321 cgaggcagtc gaccaggggc aggtggacgt gatctcggcc ttcgccaccg acggtcgcat
     4381 tccggcgctc gatctcgttg tgctcgaaga tgaccagaac ttcttcccgc cgtactgcgc
     4441 ggcgccggtc gtgcgccaag atctgctcga agagagcccg gaggtccggg acatcctcaa
     4501 ccagctcgcc ggccagatcg atgacgagac gatggccgac ctgaacttcc aaatcgatga
     4561 gggcggcacg gagccgagcg aggtggccca gaacttcctg gaggagcagg gcctgatcgg
     4621 gggcgacagc tagcccccat tccggcacga gcattcgctt ctgacaggca gcaccccgtc
     4681 ccttctcgga tggggtgctg tcatatgcgt aaatcgggac gccgatgtct gcggcgatac
     4741 cggacatgcc gccgtcccca cgtgcatggc acggactctg cgcgtggaga gggcagaggc
     4801 gagtgcacgc cctattgccg gtctcccctc ggagattcat acccgtatgt caggggcgag
     4861 cggctcgcgc cgatatcgac accaaggagg caccatcatg agccagcagc agaacatcga
     4921 tcttgtgagg ctcagcgaaa gtaacctgac cctcggcgac gagaacgagg acatccgcgg
     4981 ccgcaaggtg atcgaccgcg ccggcgacga gatcggcgag gtcgaggatc tcatgatcgg
     5041 ccgtgaagac gcgcgggtct tcttcctgct ggtcgggtcc ggcgggttcc tcggaatcgg
     5101 cgagaagacg ttcgcgatcc cggtggacgc catcacgcgg atcgacgagc agcacgtgca
     5161 tgtcgaccgc acccgcgagc atgtcgtcgg tgggcccgaa tacgatccga gcatcgtgcg
     5221 cgagggcagc gccagcggaa gcagcaattc gttctgggag gacacctacg cgcactatga
     5281 ctacgggccg ttctgggcac ctggctacac ctatccggca tatccctact acgagacggg
     5341 cggaccgggc aggcccgacg cgccgggcgt cccggatgac tacggcacgt ccggccggcg
     5401 caccggcgtc tgacaccctc aacaatcccg tacgcatagc cgacagaggg cgacgccctg
     5461 tcgagcgtcg ccctcttggc gtccgcggcg tggctctctc gtactacagc ggttggacac
     5521 tggaggtccg ccatggatca tcacctcgct cctcgcatgc actcgctcgg gcagacgtca
     5581 catctggtct tcgcctatct gtagggctcg gcctagcctg cgtcgggttg acgacctgct
     5641 cctgcaccga agtgcccctg gccccacggt cagcgcatca gctacaacac tctgccgctt
     5701 actgtgccga accgcgagcg gatgcgcgac gcgcgtggag ggattcggcg ggtcttgggt
     5761 gaggtcgtcg gccagcaagc agccgggctt tcagctccga ccgcccacca tggtcatggc
     5821 tggctcctct ggcacgcttt tcgcgacacc cgtcaaaagt gaggattcgt ccgtcgagtc
     5881 ggaagcgagg cgcgtgtgtc tagacccaag agcaatacaa cggcagggcg gttactgctg
     5941 caatcgttcg tcgtgatcgc cctgctcggc gcgct
//
LOCUS       DASYWL010000289        17692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_615685, whole genome shotgun sequence.
ACCESSION   DASYWL010000289 DASYWL010000000
VERSION     DASYWL010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 17692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17692
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615685"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(374..1615)
                     /locus_tag="VGR22_10220"
     CDS             complement(374..1615)
                     /locus_tag="VGR22_10220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEV2128981.1"
                     /translation="MPADTVQQRVDAIYRADSRRVYATLVRLFGGDFELAEDAMHEAF
                     AVAVERWSQEGMPDNPYAWLVSTGRFKAIDTIRRRNRFGEMMPELHREQEAIADIVVD
                     GESIEDDRLRLIFTCCHPALPEDARVALTLREVCGLTTEEIARAYLTAPSTLAQRIVR
                     AKTRIRKEGIPYQVPEPEELPERLDAVLRVVYLVFNEGYAASFGPSLTRPDLSDEAIR
                     LGRLLKALLGDPEATGLLALMLLNEARRPARTSPEGDLVPLEEQDRALWDHKLISEGR
                     QLVDEALATRRIGPYTLQAAIAAEHAKAPSFEATDWSRIVALYELLGMLAPSPVVELN
                     RAAAVAMRDDPDTGLTAMDAILDRGDLADYHLAHAARADLLRRLGRVPEARDAYERAL
                     QLEPQERERRLLKRRLAELEG"
     gene            complement(1615..1884)
                     /locus_tag="VGR22_10225"
     CDS             complement(1615..1884)
                     /locus_tag="VGR22_10225"
                     /inference="COORDINATES: protein motif:HMM:NF021552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEV2128982.1"
                     /translation="MCRNIRQLFNFEPPATDDEVRAAALQYVRKVSGFTKPSQTNTVA
                     FDVAVDEIAEATHRLLAALETAAPPTDRDVEAAKAGARSARRFAS"
     gene            complement(2077..2322)
                     /locus_tag="VGR22_10230"
     CDS             complement(2077..2322)
                     /locus_tag="VGR22_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128983.1"
                     /translation="MRDRRSSHTVTSRSTLTWLLIAGVLLAILAPVAGLVAAVLVGIV
                     TAVRPDLRRDLVWASLVASIISALAVITVLTAGDIFG"
     gene            complement(2501..3613)
                     /locus_tag="VGR22_10235"
     CDS             complement(2501..3613)
                     /locus_tag="VGR22_10235"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEV2128984.1"
                     /translation="MISTRRRITALTLPLAAAIAPSAVVLAQGSTSRPMAWPDETWPV
                     SGPEAHGLPADLGDRIDQQVTVTTPLLTSLLVIRGGDLVISRHYNGFAPDQTFHIWSV
                     TKSVSSIATGIAFREGLLNDVNQTLGELIPDRIPAGADPRVANITLEHLLTSTSGWAW
                     DGRINFSRHGETDDLDMMLARPMMCDSGACFEYDSTNSNLLSYIIQVQSGVTMAEYLQ
                     PRLFDPLGIPEPEWVTMYDGFNRGAGGLFLTPGDMAKLGLLYLHRGEWDGQRIVDEEW
                     VAASTSRQASGTSTITGVNIGGGGAYGYQWWVQDLFGYPAFYGNGYGDQTLYVVPDFN
                     LVVVTGVASTDVNKPENQQPVLPIIEETIVPAALEG"
     gene            complement(3792..5573)
                     /locus_tag="VGR22_10240"
     CDS             complement(3792..5573)
                     /locus_tag="VGR22_10240"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEV2128985.1"
                     /translation="MHRYRPDSSLLALRQASTRRQLLGGAATAMAGGLALHAPGITAA
                     QQPSPGSHVQAVVSSHREATAAGMAILEQGGTAADAAIAMAAVLSVVEPWFSSVLGGG
                     TWALYYDAATHSVTSMDGVGPVGGLATLEGYGERADTPGIHQAIVPGAWDGWMLWLQE
                     YGRLELGIVLDPAIRLAREGHEASAEMVTWLGRREEEVLDAPDTAAIYAPDGELIGEG
                     DLVQNPNFADSLEALARAFAQAAGEGREAAVQAARDYFYRGPLAEAIVAFSEENDGYF
                     TLEDFTTFESALLPPIAIDYGGGITVVENPPNSQGITMLLALNILKGMNISQYPHHSA
                     DAIHLQVEALKLAFADRYEYIGDPDRIDMPIDALLSDAHAEEQRQRIDMDRAMEWPID
                     GRLARVPHHTTTFHVTDREGNGAAVTTSLGAQFLVVGDTGIHINERMEFLSLEEGNAN
                     ELMPGFKVRHTSCPYMAFRGNRLWILGGNTGVDTQPQGQTQQFISVAEYGMGAQEAVN
                     QPRFVTTAFPETRYPSPIENTLQMQAGFRGSVVAELRERGHEVDLGEGTFGSANMIVI
                     TPDGTDAETGAEPDMTLSAGEVVPAGS"
     gene            5868..7151
                     /locus_tag="VGR22_10245"
     CDS             5868..7151
                     /locus_tag="VGR22_10245"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEV2128986.1"
                     /translation="MAHDRSRAMIEAAARVIPGGVSSAHRRVEPALAFTRAEGACIVG
                     ADGNPYIDYHAASGPAMLGHCHPEVNRRVAETIERIDLVGSGPGELEIALAEKIVHHV
                     PSAERVFFCNSGTEATYHAIRLARAVTGRRKLVKFQGCYHGWHDAVLMNVASPADQVG
                     EKDPLSAGMSPAVVEDTLVLRYNDVDQVRETIEREEKEIAAVILEPIAHNIGAVLPRA
                     GFLRKVRRLTAERGIVLIFDEVVTGFRHGLGGYQEVAGVTPDLTTFGKAIANGFPLAA
                     LCGRADLMDRYGPDGDVFFAGTFNAHRGSVAAGLATLGILERSGSYEHLFGLGERMRS
                     GLRHIVEERDIEATVAGFGSIFVLCFLTGPIDSYDDLLRNDADRDVRCRREMIARGVY
                     LLPVPLKRSHISLAHTAEDIDRTLDAAEAALATIA"
     gene            7285..7521
                     /locus_tag="VGR22_10250"
     CDS             7285..7521
                     /locus_tag="VGR22_10250"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEV2128987.1"
                     /translation="MAEASVKDVRNNLRAYLDRAEAGEEIVVLRRGKEVARIVPPRRE
                     PGKFPDLTEFRKSVKLRGESASETLIRMRRESRY"
     gene            7518..7952
                     /locus_tag="VGR22_10255"
     CDS             7518..7952
                     /locus_tag="VGR22_10255"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEV2128988.1"
                     /translation="MSEPVYLDSSVVVAYYVPKPNSGRVQRLYEDVEWPVISELVDLE
                     ITSALSIRSRISDLERTQARRIVNLFNEHLDAGLYARLRLQADQYRWARDATARFDLP
                     LKVPDALHLAAAQADGLQLITADRQLARNAEALDIAFELIEA"
     gene            8131..9012
                     /locus_tag="VGR22_10260"
     CDS             8131..9012
                     /locus_tag="VGR22_10260"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEV2128989.1"
                     /translation="MKVATASVNWNNADLGEGNRVPFPEILDLARAAGYTAMEYNPLF
                     GDDVATLQREAETRGITWCGTYQPIDLVSGPLHDEQLDEVALLAGRLAAIDCHDMVVA
                     EPGRPERIAIAGQVPADGSSSSLPRHAYVEIAANLHALGAVAREQGVNMHFHNHVGTW
                     IETPSEVDALLQHLDTGLVDLCFDTGHYAYGGGDALTFMQENVSRIGYLHLKDVDAAV
                     LVAARDKGWSFLEALEHVVFSPLGEGSADIPAILDTLVDNAFDGWVVVEQDTCHGDPT
                     EVARRNREFIEQQVANV"
     gene            9270..10331
                     /gene="iolG"
                     /locus_tag="VGR22_10265"
     CDS             9270..10331
                     /gene="iolG"
                     /locus_tag="VGR22_10265"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR04380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HEV2128990.1"
                     /translation="MTAKPRIALIGAGRIGVVHGRTLARISHLGTFAMIVDPREDLAR
                     QLADDYAVPKVATDFGAAIDDADIDAVVIASPTDTHAPCILAAAAADKDIFTEKPIAL
                     DLGETDEVIAAVERAGVRMQVGFQRHFDRGYQAAWEYIQSGALGQIEMLHDAMRDPEP
                     PTTEYLARSGGFWRDMVVHNFDCVRWLMGEEPVEVFAIGSTLVSNQIREIGDIDTSVV
                     TLTFASGAMATIENSRRSSFGYDVRTEVFGSTGAIFVDGARATPVRRYHREGVTEDHH
                     WFFLNRFGDAYVAELESFVSAVAHDREVAVTGADGRAALLLAYAAEMAYRDDKPVALA
                     DVEARVPDWPRLFEAPAHP"
     gene            complement(10428..11126)
                     /locus_tag="VGR22_10270"
     CDS             complement(10428..11126)
                     /locus_tag="VGR22_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128991.1"
                     /translation="MRLTTHPGRALMFLLIAIMMLPGVTAAQGTPVATPGSPEQQQAV
                     WERLDGVQQVVMRTWGDVTEDTPAPGEAPVQRFVAGLIAEFETEEQAAAALEPIRDWM
                     VASLQVNLVDVDLTAEDAEIADLGDRSMALTATGTTRDMPLTIAAALVQEGDRVLAVG
                     GSVMDEQDLLPLLEGIVDAMLEREPGGEESRDNVGRFSGGLWDIFPEQDAEVLDGMRR
                     QGDLPIYQGGTPTS"
     gene            11470..12765
                     /locus_tag="VGR22_10275"
     CDS             11470..12765
                     /locus_tag="VGR22_10275"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2128992.1"
                     /translation="MFPRFMHRAPRAAVPVYLLLSGGGALLYMSVFTVNLLYHVETVG
                     LTPLQLVLVGTILETVIMLFEIPTGVVADMVSRRLSVLIGLALIGAGFVIEGAIPAFT
                     AILASQVLWGIGYTFTSGATEAWITDEVGEAHIGPVLLRGTQAELAGTFAGIILATAL
                     GLVSIRLPIVFGGVGFIVLAVVLLRIMPEKNFRPAPKEDRSTVRHMLAAARDGFMVAR
                     SRPVVRVLLATSLLLGLASEAFDRLWAKHLIDNVGFPALPGDGDLVLWFGGIALVGTV
                     LSLVATEALKRLHPQSLEPGTPTRMLALLAGGRVVTTLGFAIAGSLPLALAMFWTRDL
                     LGSLTSPIGQAWMNRNIEPRTRATVLSIQEQVGSLGEISGGPPMGWVGNRFGVRAAIA
                     AAALVYSPVVAIYARASRLRDHVAEPAVAAEEAPPGELA"
     gene            complement(12783..13100)
                     /locus_tag="VGR22_10280"
     CDS             complement(12783..13100)
                     /locus_tag="VGR22_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128993.1"
                     /translation="MHRRHLLKTIAAGMLAPFGARISTATANEPPPLTRAVRVETGPV
                     VLRAEPSLRGHVIATIPSGCGGTTPDTPFEKADGYHWAPLTLGDAGLVGWVPTWHLSA
                     IDG"
     gene            complement(13333..13599)
                     /locus_tag="VGR22_10285"
     CDS             complement(13333..13599)
                     /locus_tag="VGR22_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128994.1"
                     /translation="MPVQYPNGHDPLGDTPEPEHDPDPKWALEDMGEDEEDDLGGGAQ
                     IPSWVIILAVLIALALLLQLGWPLVMDFLERNQDSPGFPTPGPV"
     gene            complement(13618..14181)
                     /locus_tag="VGR22_10290"
     CDS             complement(13618..14181)
                     /locus_tag="VGR22_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128995.1"
                     /translation="MAADGDHYYTQLSVNLHDDRNGIWRIPVKGGASELILPGSELAD
                     VPAPYIADVDPDCGVAVVYSYFLVGSFGPQIDRSLFWTLDLATGEKRPLEPEDMGDGS
                     SDIPARVVAASYSPDGEEVVAWVSMPSGEYDLAVIDPLTEVAMDLALPVQQEPIPPVQ
                     PQWAANDTVMLPGATGSYLLTLERIDD"
     gene            complement(14076..14816)
                     /locus_tag="VGR22_10295"
     CDS             complement(14076..14816)
                     /locus_tag="VGR22_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128996.1"
                     /translation="MRQVVGFVIMLSCLLVLPAAPGTGQVPQATPGTSPVASQGSWRI
                     SGINAIAMDGRVAAMAPDGAMLAGVSADNIFCVWETTTLLASCADEELPIRLESIVWS
                     PDGTAVVFSLDAVRMGYESDLYIYEVDAETLTNLTDDGVEGSIVSVPADTPIGDVPVW
                     SPDSLQPAFTRSVRGEEAGSTMIMRIHRASGEPEAVLRLPLLEPFAIWLPMAWLRTVI
                     TTTRSYRSTCTTTATGFGGSPSRVGPAN"
     gene            complement(15023..16945)
                     /locus_tag="VGR22_10300"
     CDS             complement(15023..16945)
                     /locus_tag="VGR22_10300"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HEV2128997.1"
                     /translation="MRGIFRFRGRRDDAGDTAVILSGRQQELLGEESRLLDRLTTALD
                     AFPVTDEDREELRRAKEQLGDLFLLVIVGEFNAGKSAFINALLAAEVMPEGVTPTTSV
                     INLLRYGDQSSETMLPDGIIQRTWPSAMLDEITIVDTPGTNAIIREHEQLTQQFVPRA
                     DMVLFVTSADRPFSESEREFMATIREWGKKIVIILNKVDLLRDPAQREQVVAFVRDNV
                     ERLLGFKPEIFPVSALLAQQAKALGDRNPGERDRLWEASEFEALEDYIIETLDEEGRI
                     RLKLLNPLGVAEHLTDRYLTATRDRLTVLEADMATIRKIERQLELYREDMREQFRYYL
                     TRIENIIHKMNDRGDEFFEETIRIGRVFDLMNTDKVRGEFERKVSGDTEREIDRTIDE
                     LIDWMVEQDLHLWETVNDFIDRRRLNKYADELIGEVGGQFRYDRREKLDNVSRRAQQE
                     VERYDAEAHARHLSMSVRNAVAAVAVAEAGAVGLGALVVAAASTAAVDVTGILAASVL
                     AGVGLVVLPIKRRQARREFYARSTELQERLLTVMREQFDSELNRSISRIEEAVAPYTR
                     FVRASQEDMEAMRRDLTSIASGLRTLRHRIEGASTDEPQLERTSSTRESTSEDGPRAL
                     ADGEPVALPEEATRDA"
     gene            17129..17593
                     /locus_tag="VGR22_10305"
     CDS             17129..17593
                     /locus_tag="VGR22_10305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2128998.1"
                     /translation="MTAQDRPSSPNGNGQHRPAHILVINDTKEILELFRELLEDEGYR
                     VSLSSFAVNDLDEVVALAPDLVILDFIIGGEEQGWQMLQKMRMDRRTARVPIIVCSAA
                     RNLVREIEGHLFAKNIGIVLKPFDIDELVAEVRRRLERDDTSQPHVATDDSA"
BASE COUNT         2875 a         5867 c         5870 g         3080 t
ORIGIN      
        1 tccgtccggc accgatccga tgtgtttcga tcccacggtg tcaccccgtg gtacctggta
       61 gtcgaactgg tcgaccggaa atcgacatca tccccggtcg atttcgaaga ttggtgttga
      121 gactcctggc tcccacgcaa cctggcgttt cgagcggagg gcgagcgata gcgagcccag
      181 agtcgagcaa tcgcgtcgat tgagaccatg ccctggctag catgacgcga tttttccacg
      241 cggcgcgctt cgcacgcctt ggtcgaaatg acggacagcc tctggggtga ttgcccttcc
      301 gacgaggcga taactccacg tcgtacgctt catgtgctcc agtcgaattg ccaagttgag
      361 ctgtgatgcc gattcacccc tccaactccg cgaggcggcg tttgaggagc cggcgttcgc
      421 gctcctgcgg ctccagctga agcgcccgct cataggcgtc tcgcgcctct ggcacccggc
      481 cgaggcgccg cagcaggtcg gcccgcgccg cgtgcgccag atggtagtcg gccagatcac
      541 cacgatcaag aatcgcgtcc atggcggtga gtcccgtatc gggatcgtca cgcatcgcga
      601 ccgccgccgc ccggttgagc tcgaccaccg gcgacggcgc cagcattccc agcagttcgt
      661 agagcgcgac gatgcgcgac cagtcggtcg cctcgaagct cggggccttc gcgtgctcgg
      721 ctgcgatcgc cgcctggagc gtgtacgggc cgatccggcg cgtcgccagc gcctcgtcga
      781 ccagctgccg tccctcagag atcagcttgt ggtcccacag cgcgcggtcc tgctcctcca
      841 gcggcaccag gtcgccctct ggcgaggtgc gcgccggacg ccgcgcctcg ttgagcagca
      901 tcaaggccaa caggcccgtc gcctcggggt cgcccagcag cgccttgagg agcctcccga
      961 gccggatggc ctcgtcggag agatcaggcc gggtgagcga tggaccgaac gaggccgcgt
     1021 agccctcgtt gaacacgagg tagaccacgc gcagcactgc gtcgagccgc tccggcagct
     1081 cctccggctc gggaacttgg taggggatgc cctccttgcg gatcctggtc tttgcgcgga
     1141 cgatccgctg ggcgagggtg gatggtgcgg tgaggtaggc gcgggcgatc tcctcggtag
     1201 tgaggccgca gacctcacgg agggtcagcg ccacgcgagc gtcttccggg agtgccgggt
     1261 ggcagcaggt gaagatcaat cgcagccgat cgtcctcgat ggattcaccg tcgacgacga
     1321 tgtctgcgat ggcctcctgc tcccggtgga gctccggcat catctcaccg aaccggttgc
     1381 gccgacggat cgtgtcgatc gccttgaagc gcccagtcga gacgagccac gcgtacgggt
     1441 tgtccggcat gccctcctgc gaccaccgct ccaccgcgac ggcgaacgcc tcgtgcatcg
     1501 cgtcctcggc cagctcgaag tcgccgccga acaggcgcac cagggtggcg tagacgcgcc
     1561 gcgagtccgc ccggtagatc gcgtcgacac gttgctgcac cgtgtcagcc ggcatcagga
     1621 ggcgaaccgc cgcgccgacc gggcgccagc cttggccgcc tccacatcgc ggtccgtcgg
     1681 cggcgctgcg gtctccagcg cggcgagcag gcgatgtgtc gcctcggcga tctcgtcgac
     1741 ggccacgtcg aacgcgaccg tgttcgtctg cgacggcttg gtgaagccgc tcaccttgcg
     1801 cacgtactgc agggccgccg cccgcacctc gtcgtcggtc gctggcggtt cgaagttgaa
     1861 cagctggcga atattcctgc acatcggtga tacctccgag caccctgcgg gtgccccggt
     1921 cgcaatgaca gatgggagct cacgccaagc tcagctgccg tgagccgccg aattcggcat
     1981 ggactcccca cgctctacgc acgcagtgaa atgagggatt cgccggtgtt gcggatgccg
     2041 ctggacgcct tcttcatggg atcgaacgga gggacatcag ccgaagatgt cgccggcagt
     2101 caggacagtg atgaccgcca gtgcgctgat gatcgaggcg accaggctgg cccagacgag
     2161 gtcgcgacgg agatctggcc gcacggcagt gacgatgccg accagcactg cggcgacgag
     2221 cccggcgacg ggcgcgagaa tcgcgaggag cacgccggcg atcagcagcc aagtaagcgt
     2281 tgaccggctg gtgactgtgt gggatgaccg ccgatccctc atggtgcaac tcctgggcga
     2341 cgatggcttc tatgcaagtc aagccaaccg tatcagaaag tcgtgaaagg tacccgcctc
     2401 gcacatgcgt ccgggaagac tctcgtgcct gaagacaaaa cggaccgaca ccaagccggt
     2461 ccgttcgcac ggcttgcgcc gatatgggtt gagtttcgtg tcagccctcg agggcagcgg
     2521 ggacgatcgt ctcttcgatg atcggtaaca ctggctgctg gttctccggc ttgttgacgt
     2581 cggtactggc cacacccgta acgaccacga ggttgaagtc cggcaccacg tagagggtct
     2641 ggtcaccgta gccgttgccg tagaaggcgg ggtagccgaa gagatcctgc acccaccact
     2701 ggtatccgta ggctcccccg ccgccgatgt tgacgcccgt gatcgtgctc gtgccgctgg
     2761 cctgtcggct cgtcgaggcc gcgacccact cctcgtcgac gatgcgctgc ccgtcccact
     2821 cgccgcgatg gaggtacaac aggccaagct tcgccatatc gcctggggtg aggaagaggc
     2881 cacccgcgcc ccggttgaac ccgtcgtaca tggttaccca ctccggctcg ggaatgccga
     2941 gcggatcaaa gagccggggc tgtaggtatt ccgccatggt gacgccactc tgtacctgaa
     3001 tgatgtacga gagcaggttg ctgttggtgg agtcgtactc aaagcaggcg ccggagtcgc
     3061 acatcatcgg cctagcaagc atcatatcga ggtcgtccgt ttcgccgtgg cggctgaagt
     3121 tgatgcgtcc gtcccacgcc cagccgctgg tcgaggtgag caggtgctcg agcgtgatgt
     3181 tcgcgacccg tgggtcggcc ccagccggga ttcgatccgg gatcagctcg ccgagggtct
     3241 ggttcacgtc gttgaggagc ccctctcgga aggcgattcc agtcgcgatc gagctgacgc
     3301 ttttggtcac agaccagata tggaaggtct ggtcgggtgc gaagccgttg tagtgccggc
     3361 taatgacaag atccccgcct cgaatcacga gcagcgaggt gagtagcggt gtcgtgactg
     3421 tgacctgctg gtcgatccgg tcgccgaggt cggccgggag cccatgcgcc tctgggcccg
     3481 agacaggcca cgtctcatcg ggccaggcca tcggcctgga ggtagagcct tgtgccagca
     3541 cgacggcgga tggggcgatc gctgcggcca gtggcaaggt cagggcggtg atgcgtcgtc
     3601 gtgtggaaat catgaactga atcttctcca tgggcctttg tgctgatgat tgtgtgggcc
     3661 gatcgcgcct gctcctgctc gcaaccctga ggtcgagagg taccgtggcg cgcggcagca
     3721 tcaccgacgc gtcggccatg gcacaacgcg tcggtcctgg tttgcggtga cgggagcggg
     3781 gtgcgacgga gctacgaccc ggcggggacg acctccccgg ccgagagggt catgtccggc
     3841 tcagcgccgg tttcggcgtc ggtgccgtcc ggcgtgatga cgatcatgtt ggcggagccg
     3901 aaggtgccct cgccgaggtc gacctcgtgc ccgcgctctc gcaactcggc gacaacgctg
     3961 ccccggaagc cggcctgcat ctgcaacgtg ttctcgatcg gcgaggggta gcgcgtctcc
     4021 gggaaggcgg tggtcacgaa gcgtggctgg ttgactgcct cctgcgcgcc catgccgtac
     4081 tccgccacgc tgatgaactg ctgcgtctgc ccttgcggct gcgtgtcaac gccggtattg
     4141 ccgccgagga tccagagccg gttgccccgg aaggccatgt acgggcagga ggtatgccga
     4201 accttgaagc ccggcatgag ctcgttggca ttgccctcct ccagcgagag gaactccatg
     4261 cgctcgttga tatggatgcc ggtgtcgccg acgacgagga actgcgcgcc gagactcgtg
     4321 gtgaccgccg cgccgttgcc ctctctatcg gtgacgtgga aggtggtggt gtggtgcggc
     4381 acccgggcca acctaccgtc gatcggccac tccatcgcgc ggtccatgtc gatgcgctgt
     4441 cgttgctcct cagcgtgggc gtccgagagg agcgcatcga tcggcatgtc gatgcgatcg
     4501 ggatcgccga tgtactcgta gcggtcggcg aaggcgagct tgagcgcctc gacctgcagg
     4561 tggatggcat cggcgctgtg gtgcggatac tggctgatgt tcatcccctt caggatgttg
     4621 agggccagca gcatggtgat gccctggctg ttgggcgggt tctcgacgac cgtgatgccg
     4681 ccgccgtaat cgatcgcgat gggcggcagg agcgcagact cgaaggtggt gaaatcctcg
     4741 agggtgaagt agccgtcgtt ctcctcggag aaggcgacga tcgcctcggc aagcggacca
     4801 cggtagaagt agtcgcgggc agcctgcacc gccgcctcgc gcccctcgcc ggcggcttgg
     4861 gcgaaggcgc gggcgagcgc ctcgagggag tcggcgaaat tggggttttg tacgagatcc
     4921 ccctcgccga tcagctcgcc gtccggggcg tagatcgcgg cggtgtcggg ggcgtcgagc
     4981 acctcctcct cgcggcgtcc gagccaggtg accatctccg cgctggcctc gtgcccctcg
     5041 cgcgcgagcc ggatggcggg gtcgaggacg atgccgagct cgaggcgacc gtactcctga
     5101 agccagagca tccagccgtc ccacgcgccg ggcacgatcg cctggtggat gccgggggtg
     5161 tcggcgcgct ccccgtaacc ctctagggtc gccaagccgc cgaccgggcc gacgccatcc
     5221 atactggtga cgctgtgagt ggccgcatcg tagtagagcg cccaggtgcc gccgccgagg
     5281 acgctggaga accacggttc gaccacgctg agcaccgcgg ccatggcgat ggcggcatcg
     5341 gcggcggtgc cgccctgttc gaggatcgcc atcccggcag cggtggcttc tcggtgggag
     5401 gagacgaccg cctggacgtg cgagccgggg ctcggctgct gggcggcggt gatgccgggt
     5461 gcatggagcg cgaggccgcc ggccatggca gtcgcggcgc cgccgagcaa ctggcgccgg
     5521 gtgctggcct gacggagggc cagcagggac gaatcggggc gatacctgtg catgctgcag
     5581 ggctccctgt ggggcaatca cgatcaacgt agcaagtata cgggccaagc tctcaatcga
     5641 agggagacac gggggatcac gagcggcgaa cgctcgtggt gaagcggcct gccaccctga
     5701 ccgcccgggc ggctgccgtt tccaggccac cggtcgcttg cagcacgatc accgtcaggc
     5761 acgcgcctcg cggagtttgg ttggacgggc tcggttgccc ttgcgtttcg ccgacccatt
     5821 cctgatactg ccgccattca gtgggtgtgc agtgatgggg agacgccgtg gcgcatgatc
     5881 ggtcccgagc gatgatcgag gcggcggcga gggtgatccc cggcggggtg agcagcgccc
     5941 accggcgcgt ggagccggcg ctggccttca cccgcgccga gggcgcctgc atcgtcggcg
     6001 ctgacggcaa cccgtacatc gactaccatg cggcgtccgg cccggcgatg ctcgggcact
     6061 gccaccccga ggtcaaccgg cgggtcgccg agacgatcga gcgcatcgac ctcgtcggct
     6121 cggggccggg cgagctggag atcgcgctgg cagagaagat tgttcaccac gtgccctccg
     6181 ccgagcgggt gttcttctgc aactcgggca ctgaggcgac ctaccacgcg atccgcctcg
     6241 cccgtgccgt caccggccgt cgcaagctgg tcaagttcca ggggtgctac cacgggtggc
     6301 acgacgccgt gctgatgaac gtcgccagtc ccgccgatca ggtgggggag aaggacccgc
     6361 tctcggcggg gatgtccccg gccgtcgtcg aggacacgct ggtgctgcgg tacaacgatg
     6421 ttgaccaggt gcgcgagacg atcgaacggg aggagaagga gatcgcggcg gtgatcctcg
     6481 agccgatcgc ccacaacatc ggcgcggtgc tgccccgcgc cggcttccta cgcaaggtcc
     6541 gccggctgac ggccgaacgc ggcatcgtgc tgatcttcga cgaggtcgtg accgggttcc
     6601 ggcacgggtt gggcggctac caggaggtgg cgggggtgac acccgacctg acgaccttcg
     6661 gcaaggcgat cgcgaacggc ttcccgctcg cggcgctttg cggccgcgcc gacctgatgg
     6721 accgctacgg ccccgacggc gacgtcttct ttgccggcac cttcaatgcc caccggggca
     6781 gcgtcgccgc cggtctcgcg acgctcggga tcctggagcg ctccggcagc tacgagcacc
     6841 tgttcggcct cggcgagcgg atgcggtccg ggctccgtca catcgtcgag gagcgcgaca
     6901 tcgaggcgac ggtggccggg ttcgggtcga tcttcgtgct ctgtttcctc accggcccga
     6961 tcgacagcta cgacgatctg ctccgcaacg acgccgaccg cgacgtccgg tgccgccgtg
     7021 agatgatcgc gcggggcgtt tacctgctgc cggtgccgct caagcgcagc cacatcagcc
     7081 tcgcccatac tgctgaggat atcgaccgca ccctcgacgc cgccgaggcc gccctggcga
     7141 ctatcgcctg agcaaagctg ggtacctcgg aggcgaccca gatgtcgccc cgtgctccgg
     7201 cggatccgta cgatgatacc gatccgctct caaccctcag gtgtgcactt cgtgtgtacg
     7261 ctggtgtcga aggaggagtg agccatggca gaggcgagcg tgaaggacgt gcgcaacaac
     7321 ttgcgggcct atctcgaccg tgccgaggcc ggcgaggaga tcgtcgtcct gcgccgcggc
     7381 aaggaggtcg cgcggattgt gccgccgcga cgggagcccg ggaagtttcc ggatctcacg
     7441 gagttccgca agtcggtcaa gctccggggc gagtcggcga gcgagaccct aattcggatg
     7501 cggcgggagt cgcggtattg agtgaaccgg tctatctcga ttccagtgtc gtggtcgcct
     7561 actacgtgcc caagccaaac agtgggcgcg tgcaacggct gtacgaggat gtcgagtggc
     7621 ccgtcatcag tgagctggtg gacctggaga tcacctccgc gctctcgatc aggagccgaa
     7681 tcagtgacct ggagcgcacg caggctcgtc ggatcgtgaa cctcttcaac gagcatctgg
     7741 acgccgggct ctacgcgcgg cttcgtctgc aagccgacca gtaccggtgg gcgcgagacg
     7801 ccaccgcgcg cttcgacctg ccgctgaaag tgccagacgc cctgcacctg gccgccgcgc
     7861 aggccgatgg tcttcagctg atcaccgcgg atcggcagct tgcccggaac gccgaagccc
     7921 tcgatatcgc gttcgagcta atcgaagcct aatgcgaccg ctccactcgt cgctcgcacc
     7981 agttgcgctc cggacacatg ttcccgatac tgaccgcatt ccatcgggag gcatgaggca
     8041 cccacgcgat gccaagagtg ctgggtgata cgttccgacc atgacggcgc ctcacctcat
     8101 gtgacacgtc ggctctggag gctgctcata gtgaaggtcg cgaccgcctc ggtgaactgg
     8161 aacaacgctg atctcggcga ggggaaccgc gttcccttcc ccgagatcct cgaccttgcc
     8221 cgcgcggcgg gctacaccgc catggagtac aacccactct tcggcgacga tgtcgccacg
     8281 ctgcaacgcg aggccgaaac gcggggcatc acctggtgcg gcacctacca gccgatcgac
     8341 ctcgtctccg gtccgctcca tgacgagcag ctcgacgagg tcgcgctgct ggccggtcga
     8401 ctggcggcaa tcgactgcca tgacatggtc gttgccgagc caggtcgacc cgagcggatc
     8461 gcgattgctg ggcaggtccc ggcggacggc tcgtcctcct ccctgccgag gcacgcctac
     8521 gtggagattg ccgccaacct gcacgcgctt ggggccgtcg cgcgggagca gggggtcaat
     8581 atgcattttc acaaccatgt cggcacctgg atcgagacgc cgtccgaggt cgacgccctg
     8641 ctgcaacacc tggacaccgg gctggtcgat ctctgtttcg acaccgggca ctatgcctac
     8701 ggcggcggtg acgcgctgac cttcatgcag gagaacgtca gccggatcgg ctacctgcac
     8761 ctgaaggatg tcgacgccgc ggtgctggtg gcggcccggg acaagggatg gagctttctc
     8821 gaggcgttgg agcacgtggt tttctcgccg ctgggcgagg ggtcggcgga cattcccgcg
     8881 atcctcgaca cgttggtgga caatgcgttc gacggctggg tggtggtcga gcaggacacc
     8941 tgtcatggtg atcccactga ggttgcccgg cggaaccggg agttcatcga gcagcaggtc
     9001 gctaatgtgt aaagtagtaa cgctgcagcc tgccgagaca acccgaaatg gaagtcatcc
     9061 tgagcggagg ccgaaggccg aagtcgaaga atctcccgtc cctgtcaacg gcagggacga
     9121 tccgcgctct cacgcagtcg cccttgggtc tcgatggaga gcggatcgcg ggaagtaccg
     9181 gggcaagcgc cggggattct tcgcttcgct cagaattaca ccttcttgcg taatgccaaa
     9241 ttggtgactc tgaggagggc cgcgaagaga tgacagccaa acccaggatc gcactcatcg
     9301 gagcgggccg gatcggggtg gtgcatggac gcacgctcgc gcgtatcagt cacctcggga
     9361 cgttcgcgat gatcgtcgat cctcgcgagg atctggcgcg ccagttggcc gatgactacg
     9421 ctgtgccgaa ggtcgccacc gactttggcg cggcgatcga cgacgccgac atcgatgccg
     9481 tggtgatcgc ctcgccaacc gacacccacg cgccttgcat tctcgcagct gccgcagccg
     9541 acaaggatat cttcaccgag aaaccgatcg cgctcgacct cggcgagacg gacgaggtga
     9601 ttgctgccgt ggagcgagcc ggggtgcgga tgcaggtcgg gttccagcgg cacttcgacc
     9661 gcggctacca ggcggcgtgg gagtacatcc agagcggcgc gctcggacag atcgagatgc
     9721 tccacgacgc gatgcgcgac cccgagccgc cgacgacaga gtacctcgcc cgctcgggcg
     9781 ggttctggcg cgacatggtg gtccacaact tcgactgcgt gcgctggctg atgggcgagg
     9841 aaccggtcga ggtctttgcg atcggctcga cactcgtcag caaccagatc cgcgagattg
     9901 gggacatcga cacctcggtc gtgacgctta ccttcgcctc cggcgcgatg gccacgatcg
     9961 aaaacagccg acggtcgtcg ttcggatacg acgtgcgcac ggaggtgttc ggctcaacgg
    10021 gcgcgatctt cgtcgacggt gcccgggcga cgccagtgcg gcgctaccac cgggagggcg
    10081 tgacggagga ccaccactgg ttcttcctga accgcttcgg ggacgcctat gtggccgagc
    10141 tggaatcgtt cgtctcggcc gtcgcgcacg accgggaggt cgcggtgacc ggcgcggacg
    10201 gccgggcggc gctgctgctg gcctacgcgg ccgagatggc ctatcgggac gacaagccag
    10261 tggcgctggc cgacgtcgag gcccgcgtcc ccgactggcc caggctgttc gaggcaccgg
    10321 cccatccata ggggaggacg ccggcgtcct cccctatgga tgggccggat ttggtgctga
    10381 ctccaatgga tgcttggatg ccgtggcggt tgccgggacc gtcgggatca gcttgtcggc
    10441 gtgccgccct ggtagatcgg caggtcaccc tgccggcgca tgccgtccag cacctcggcg
    10501 tcctgctctg ggaagatgtc ccagagacca ccgctgaagc gcccaacgtt gtcgcgcgac
    10561 tcctcgccgc ccggctcgcg ctcgagcatc gcatccacga tgccctccag caatggcagc
    10621 aggtcctgct cgtccattac cgagccgcca acggccagca cgcggtcacc ctcctgcacc
    10681 aacgccgcgg cgatcgtgag cggcatgtcg cgcgtggtgc ctgtggctgt caacgccatc
    10741 gacctgtcac caaggtcggc gatctcggcg tcttcggccg tcaggtcgac gtcgaccagg
    10801 ttcacctgca gtgacgcgac catccagtcg cggatcggct ccagtgccgc ggccgcctgc
    10861 tcctccgtct cgaactcggc gatgaggccg gccacgaacc gctgcaccgg cgcctcgccc
    10921 ggcgcgggag tgtcttccgt cacgtcaccc cacgtgcgca tcaccacctg ctggacgccg
    10981 tcgagccgct cccagaccgc ctgctgctgc tcgggcgatc ccggtgtggc gaccggggtt
    11041 ccctgagcag cggtcactcc tggaagcatc atgatcgcga tcaggaggaa catcagtgcc
    11101 cgccccggat gggttgtcaa acgcatgctt catctccttc cgcccaagat gcgcaccagg
    11161 cgatcctgtc ccagtgaaat gtacggacag tatctacacc tatcgtagca ggtttttgcc
    11221 cggatcggca cgcgcaccgg ttcccatcgc atccacaccg ccctatactg ggcaggagat
    11281 cgttcggaac acgggcagag aggtatatcg atgccagtcc tcaccatgca cgtgtcccgc
    11341 cgcaccgccg gctagcgact ccgctccggc ctgttgccag ggaccgtccc accatcccga
    11401 tgtcgccgcg ggtatcgccc gctggcggtc tggttgtgtc cctgacactg ttccggagtg
    11461 taccaacccg tgtttccccg attcatgcac cgcgcgcccc gcgcggctgt gcctgtctac
    11521 ctgctcctga gcggcggtgg cgcgttgctc tacatgagcg tcttcaccgt caacctgctc
    11581 tatcacgtcg agaccgtcgg cctcacaccg ctccagctgg tgctcgtcgg caccatcctg
    11641 gagaccgtca tcatgctctt cgagattccc accggcgtgg tcgccgatat ggtgagccgg
    11701 cggctgtcgg tactgatcgg cctcgcgctc atcggtgccg gcttcgtcat cgagggagcg
    11761 atccctgcct tcaccgccat tctcgcctcc caggtgctgt ggggcatcgg ctacaccttt
    11821 accagcggcg ccaccgaggc gtggatcacc gatgaggtgg gggaggcgca catcgggccg
    11881 gtgctgctgc gcggtacgca ggccgagctg gccggcacgt tcgccggcat catcctcgcc
    11941 accgcgctcg ggctcgtctc gatccggctc ccgatcgtgt tcggcggtgt cgggttcatc
    12001 gtgctcgctg tcgtgctgct ccggatcatg ccggagaaga acttccgtcc tgccccgaaa
    12061 gaggatcgct ccactgtccg gcacatgctg gcggccgccc gcgacggatt catggtggcc
    12121 cgcagccggc cggtcgtgcg cgtcctgctg gcgacatccc tgctgcttgg gctcgccagc
    12181 gaggcgttcg accgcctctg ggccaagcac ctgatcgaca acgtcggctt cccggccctg
    12241 cctggcgatg gcgacctcgt gctctggttc ggcgggatcg cactcgtcgg tacggtcctc
    12301 tcgctcgtcg ccaccgaagc tctcaagcgg ctccacccgc agtcgcttga gcccggcacg
    12361 ccgacgcgca tgctcgccct gctcgccggt gggcgcgtgg tcaccacact cggcttcgca
    12421 attgcggggt cgctgccgct ggcgctcgcc atgttctgga cccgcgacct gctgggcagc
    12481 ctgacctctc cgatcggcca agcgtggatg aaccggaaca ttgagccccg cacccgtgcg
    12541 acggtgctct ctatccagga gcaggtcggg tcgctcggcg aaatcagcgg cgggccgccg
    12601 atgggatggg tcggcaaccg cttcggcgtg cgagcggcga tcgctgcggc cgcgctggtg
    12661 tactcgccag tcgtcgccat ctacgcgcgc gcctctcggt tgcgcgacca tgtggccgag
    12721 cctgcggtcg ccgccgagga ggcgccgccc ggcgagttgg cgtaagtcct gaccggtggg
    12781 ggtcagccgt cgatggcgga gaggtgccag gtcggcaccc agccaaccag cccggcatcg
    12841 ccgagggtga gcggtgccca gtgatagccg tcagcctttt cgaacggggt gtccggcgtg
    12901 gttccgccgc agccggaggg gatcgtggcg attacatgcc cccgcagcga tggctctgcg
    12961 cgcagcacca ctggtccggt ctccacgcga acagcgcgcg tcaacggtgg gggctcattg
    13021 gcggtcgccg tgctgatccg tgcgccaaag ggcgcgagca taccggccgc gatggttttc
    13081 aggagatggc gtcggtgcat cgattggtcc gggggcgtct gtatcgcaat gcaaggcgag
    13141 gtgtccaaat atgctttcca cagcgtagca tccccgagcc gctcgccgag tgcattgcgg
    13201 cacaccccat tcagctcatt cgtccggggc cagagcagat gcaggggccg accactccct
    13261 acgggagatc ggcccctgga cgtggcatcc gttgcaacag gcccgttcgc gttaggcaca
    13321 gcatggccgt cgtcatacgg gtccgggtgt agggaagccc ggcgagtcct ggttgcgttc
    13381 gaggaaatcc atcaccaacg gccagccgag ctgcagcaac agcgccagcg cgatgagcac
    13441 cgccaggatg atcacccacg acgggatctg cgctccgccg ccgaggtcgt cttcctcgtc
    13501 ctcgcccatg tcctccagcg cccacttcgg gtccggatcg tgctctggtt ccggcgtatc
    13561 gccgagcgga tcgtggccgt ttggatattg gacgggcatc cacgctcctt cgttctgcta
    13621 gtcgtcgatg cgctccagcg tcagcagata tgaaccggtc gcgcccggca gcatcaccgt
    13681 atcattcgcg gcccattgcg gctgcaccgg cgggatcggt tcctgctgca ccggcaacgc
    13741 aaggtccatc gccacttcgg tcagtgggtc gatcactgcc agatcgtact caccggacgg
    13801 catcgacacc cacgcgacca cttcctcacc gtccggtgag taactggcgg cgacaacgcg
    13861 cgctggtata tccgacgagc catcgcccat gtcctccggc tcgagcggcc tcttctcgcc
    13921 tgtggccaga tccagagtcc agaagagcga ccgatcgatc tgcggcccga agctacccac
    13981 caggaaatac gagtagacga cggcaacacc acaatctggg tcgacatcgg cgatgtaggg
    14041 agcgggcaca tcggccagct ccgagccggg caggatcagt tcgctggccc cacccttgac
    14101 ggggatccgc caaatcccgt tgcggtcgtc gtgcaggttg accgatagct gcgtgtagta
    14161 gtgatcaccg tccgcagcca cgccatcggc agccagatcg cgaacggctc caacagcggc
    14221 aggcgcagga ccgcctcggg ctcgccactg gcacgatgga tgcgcatgat catcgtcgag
    14281 ccggcttcct cgccgcgcac cgatcgcgtg aaggccggct gcaggctgtc cggcgaccac
    14341 actggcacat cgccgatcgg cgtgtcggcc gggacactga ctatcgagcc ctcaacccca
    14401 tcgtcggtca ggttcgtcag cgtttccgca tcgacctcgt agatgtagag atcgctctcg
    14461 tagcccatgc ggacggcgtc cagcgagaac accacggcgg tgccatccgg cgaccagaca
    14521 atcgactcca gtcggatcgg cagctcctcg tcggcgcaac tcgccaggag cgtagtggtc
    14581 tcccagacgc agaagatgtt gtcggcgctc acccccgcca gcatggcgcc gtccggcgcc
    14641 atcgcggcca cccggccgtc catcgcgatg gcgttgatcc cggatattcg ccacgaccct
    14701 tgactggcga caggagacgt ccccggcgtc gcctggggaa cctgcccggt tcccggcgcg
    14761 gcaggcagca ccaggaggca agacagcata atcacgaaac cgacaacttg tcgcatccat
    14821 ggctcctgga ttatcggaca atccagccgg tgtgatactg gcccattggc gcacgtagtc
    14881 gacgttcaac acggcaaccg gtcatttcgg ccctgttcga ctctggcctg cgggcggacg
    14941 ggcctcatgt ccgccctgta ggagtaggct ctgctcacga aggggacgga aaggaatcat
    15001 catcctccca gcacaaacgg catcacgcat cgcgcgtcgc ctcctccggc agggcgaccg
    15061 gttcgccgtc ggcaagcgcg cgcgggccat cctccgatgt cgactccctt gtcgatgacg
    15121 tgcgctccaa ctggggctcg tctgtggatg ccccttcgat ccggtgccgc agcgtcctca
    15181 acccactggc gatgctggtc aggtcgcgcc gcatggcctc catgtcctct tgtgaggcac
    15241 ggacgaagcg cgtgtagggc gcaaccgcct cctcgatgcg actgatcgaa cggttcagct
    15301 cgctgtcgaa ctgctcccgc atcacggtca gcagccgctc ctgcagctcc gtggagcgcg
    15361 cgtagaactc ccgccgcgcc tgccgccgct tgatcggcag caccaccagc ccgactccgg
    15421 cgaggacgct ggcagcgagg ataccggtga catcgaccgc ggccgtgctt gccgcggcga
    15481 ccaccagcgc cccgaggccg accgcgcccg cctccgcgac ggcgaccgcc gccacggcgt
    15541 tgcggaccga catggacaga tgccgcgcgt gcgcctccgc gtcgtagcgc tcaacctcct
    15601 gctgcgcgcg ccgggagacg ttatccagct tctcccggcg gtcgtaacgg aactgcccgc
    15661 cgacctcgcc gatcagctcg tcggcgtact tgttgagtcg tcgccggtcg atgaagtcgt
    15721 tgacggtctc ccagaggtgg agatcctgct cgaccatcca gtcgatcagc tcgtcgatgg
    15781 tccggtcgat ttcccgctcg gtatcgccgg aaaccttgcg ctcgaactcg ccgcgaacct
    15841 tgtcggtgtt catcaggtcg aagacccgcc cgatacggat cgtctcctcg aagaactcgt
    15901 cgccgcggtc gttcatcttg tggatgatgt tttcgatgcg ggtcaggtag tagcggaact
    15961 gctcgcgcat gtcctcccgg tagagctcga gctggcgctc gatcttgcgg atcgtcgcca
    16021 tgtctgcctc gagcacggtg aggcggtccc tcgtggcggt gagatagcgg tcggtgaggt
    16081 gctcggccac gccgaggggg ttgagcagct tgaggcgaat gcgcccttcc tcgtcgagcg
    16141 tctcgatgat gtagtcctcc agcgcctcga actcgctcgc ctcccaaaga cggtcacgct
    16201 cgccggggtt gcggtcgccg agcgccttgg cctgttgcgc aaggagggca gagaccggga
    16261 agatctcggg cttgaagccc agcagccgct cgacgttatc ccgcacgaac gcgacgacct
    16321 gctcgcgctg ggcgggatcg cgcaggagat cgaccttgtt gaggatgatg acaatcttct
    16381 tgccccactc gcggatcgtc gccatgaact cccgctcgct ctcgctgaac gggcggtcgg
    16441 cgctggtcac gaagagcacc atgtcggcgc gggggacgaa ctgctgcgtg agctgctcgt
    16501 gctcgcggat gatcgcgttg gtgccagggg tgtcgacgat ggtgatctcg tcgagcatcg
    16561 ccgacggcca cgtccgctgg atgatgccgt ccggcagcat cgtctcgctc gactggtcgc
    16621 cgtagcggag caggttgatc accgaggtcg tgggagtgac cccctccggc atgacctcgg
    16681 cggcgaggag cgcattgatg aaggccgact tgccggcgtt gaactcgccg acgatcacga
    16741 gcaggaagag gtccccgagc tgctccttgg cgcgccgcag ctcctcgcga tcctcgtcgg
    16801 ttacggggaa ggcatcgagt gccgtggtga ggcggtcgag caggcgcgac tcctccccca
    16861 gcagctcctg ctgccgaccg gagagaatta ccgccgtgtc gccggcgtcg tctcgccgtc
    16921 cccgaaagcg aaagatccct cgcatcaagc cgttgctcct cgtggcccgc gccgccattc
    16981 gactgagaaa ttggccgaaa ctgcgcggaa tcactcgatg tctagagtac aatgatccca
    17041 cacaccaatg tgcgttcggg tcatgcacgt catgatccgc attccggctg ccacgatcgt
    17101 gacgccgccg caaggagcct cactgcccat gaccgctcag gatcgcccga gttccccgaa
    17161 tgggaacggt cagcaccgtc cagcgcatat attggtgatc aatgacacga aggagatcct
    17221 cgagctcttc cgcgaactgc tggaggacga gggctaccgc gtgtcgcttt cctccttcgc
    17281 ggtgaacgac ctcgacgagg tggtggcgct ggcgccggac ctggtgattc tcgacttcat
    17341 catcggtggt gaagaacagg gctggcagat gctccagaag atgcggatgg accgccgtac
    17401 cgccagggtc cccatcatcg tctgcagtgc tgcacgcaat ctggtgcggg agatcgaagg
    17461 gcacctgttc gcgaagaaca tcggcatcgt gctgaagccc ttcgacatcg acgaactcgt
    17521 cgccgaggtg cgaaggcggc tagagcgcga tgacacatct cagcctcatg tcgcgaccga
    17581 cgactccgca tgatgacgcg atcgggagcg ctgcgctcat gactttcccc actgtgcagg
    17641 ccgaccgccg cagcgacgtg ctcatcgtcg gaggcggtgt cactggctgt tc
//
LOCUS       DASYWL010000290         3683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_222014, whole genome shotgun sequence.
ACCESSION   DASYWL010000290 DASYWL010000000
VERSION     DASYWL010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222014"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VGR22_10310"
     CDS             <1..297
                     /locus_tag="VGR22_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2128999.1"
                     /translation="LAQAVTYDLNGIRAKLIDVRMRGPQLGPSGLHEKFNALLDSVDG
                     ADYAPPQQAREVYAELAGQLDEVIGQLKDIVEGKVSELNNAVQAIGLPVAGRAG"
     gene            450..1919
                     /gene="pruA"
                     /locus_tag="VGR22_10315"
     CDS             450..1919
                     /gene="pruA"
                     /locus_tag="VGR22_10315"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR01237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HEV2129000.1"
                     /translation="MRGRTARTAIPAHHRREPRETGQWIRSINPGNLDQVVGEVAKAR
                     PADVEDAIAAAEEAFASWRKMPAEGRASVLLRMAGILRRRRLELASWLVYELDKAWDE
                     AEGEVAEAVDFLEWYARQALELDAPVDLARLPDEATDYRYQPIGVGVVIPPWNFPCAI
                     FTGMTMAPVAAGNTVIVKPASNTPVIGYKMFEIMVEAGVPAGVVNFLPGEGSEIGDAL
                     VDHPRTRFVAFTGSKDVGIRIYERAAKVQPGQRWLKRVTAEMGGKDAIIVDADADLEA
                     AIEGIITSTFGFQGQKCSACSRAIVHADVYDEVVAGIVERTRQSVKSGAGLSGEATVG
                     AIADSKQYESIKQYIEIGKSEGRLVFSGETPDANGYYIPPTIFADVAPDARIAREEIF
                     GPVLAIVKARDFDEALEIVNDSEYGLTGSVYSRNRDHLERARDEFDVGNLYFNRKCTG
                     AMMGVHPFAGIKLSGTNSKAGGPEYLYNFVEAKAIGERL"
     gene            1958..2902
                     /locus_tag="VGR22_10320"
     CDS             1958..2902
                     /locus_tag="VGR22_10320"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEV2129001.1"
                     /translation="MSSRAALALNPVFRKGILLATSNPVVSRSVHRYGMRLGASRFVA
                     GETLDECIPVLKGLNAKGLRINTTILGEDVGDEAAAAAVADAYVEVLDRLAVEQINGN
                     VALKLTHLGLNLGEEIAYQNVARLVEYADTLNNFIRIDMEESAWVDATLRIYRRLREQ
                     GHDNVGTVLQAYLYRTEQDLTDLLPLEPNLRLVKGAYLEPDSIAFARKSDVDANYLRL
                     AERMLAGGGFTAIATQDERIIEHVINLTEREAIPRDRFEFQMMYGIRPQLQLDLVQQG
                     YRVLVATPYGPDWYHYLMRRLAERPANLLFFARNLVRR"
     gene            complement(2959..3150)
                     /locus_tag="VGR22_10325"
     CDS             complement(2959..3150)
                     /locus_tag="VGR22_10325"
                     /inference="COORDINATES: protein motif:HMM:NF024776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2129002.1"
                     /translation="MKQYSVDLRERLLVATDAGLSQAEAARIFGVGTSTISRWRWPPS
                     PPPTPGAGSPTAATRSQPI"
BASE COUNT          712 a         1104 c         1239 g          628 t
ORIGIN      
        1 cttgcacagg cggtgacgta cgacttgaac gggattcggg ccaagctgat cgatgtgagg
       61 atgcgagggc cgcaactcgg gccgagcggc ctgcacgaga agttcaacgc cctgctcgat
      121 tcggtcgacg gcgccgacta cgctccgcct cagcaggcgc gggaggtcta cgcggagctt
      181 gccggccaac tcgatgaggt aatcggccaa ctcaaggaca ttgtcgaggg caaggtatcc
      241 gagctcaaca atgcggtgca ggccatcggc ctgccggtgg caggacgggc cggatagctg
      301 ctcaacgccg gctctgtcaa cgcttgactg gctgcggcct gcagcgcagc ggaaaggaca
      361 aggatcagga tgcgcactcc attcacaaac gaaccctcgc tcgacttctc gcttgaggag
      421 acccgggcgg cgatgcggga tgccctgaaa tgcgtggaag gacagctcgg acagcgatac
      481 ccgctcatca tcggcgggag ccgcgcgaga ccgggcaatg gatccgctcc atcaatccgg
      541 gcaacctcga tcaggtcgtc ggggaagtcg ccaaagcccg tccggccgat gtcgaggatg
      601 cgatcgcggc cgcagaggag gcatttgcgt cgtggcggaa gatgccggcg gagggacggg
      661 cgagcgtcct gctccgcatg gcgggaattc tgcggcgacg gcggctcgaa ctggcgagct
      721 ggctggtgta tgagctggac aaagcctggg atgaagccga gggcgaggtc gccgaggcag
      781 tcgacttcct ggagtggtat gcccgccagg cactggagct cgacgcgccg gtcgacctcg
      841 cccgcctgcc ggacgaggcg accgactatc gctaccagcc gatcggcgtg ggcgtcgtca
      901 tccccccatg gaattttccg tgcgcgatct tcaccggcat gaccatggcc cctgttgccg
      961 ccggcaacac ggtcattgtg aaaccggcaa gcaacacacc ggtcatcggc tacaagatgt
     1021 tcgagatcat ggtggaggcg ggagtgcccg ccggtgtcgt caacttcctt cccggggagg
     1081 ggagcgagat cggcgacgcg ctggttgacc acccacgaac gcggtttgtc gccttcacgg
     1141 ggtcgaagga cgttgggatt cggatctacg agcgggccgc gaaggtccag cctggtcagc
     1201 gctggctgaa gcgtgtcacg gcagaaatgg gtggcaagga cgcgatcatc gttgacgccg
     1261 atgccgacct cgaggcagcc atcgagggga tcatcacctc gacctttggc ttccagggcc
     1321 agaaatgctc ggcctgctcc cgcgcgatcg tccacgctga cgtctacgat gaggtcgtcg
     1381 ccggtatcgt cgagaggacc cggcaatcgg tcaagagtgg ggcagggctc agtggtgagg
     1441 caaccgtcgg tgctatagcc gacagcaagc agtacgagtc gatcaagcag tacatcgaga
     1501 tcggcaagag cgaagggcgc ctggtattca gcggcgagac cccggatgcc aacgggtact
     1561 acatcccacc caccattttc gccgatgtgg ctcccgacgc gcgcatcgcc cgtgaggaga
     1621 tcttcggtcc ggtgctcgcg atcgtgaagg cgcgcgactt cgacgaggcg ctggagattg
     1681 tcaacgattc ggagtacggg ttgaccggat cggtctattc gcgcaatcgg gaccatctcg
     1741 aacgggcacg ggacgagttt gacgtgggta atctctactt caaccggaag tgcaccggtg
     1801 ccatgatggg ggtccatccc ttcgccggca tcaagttgag tgggacgaac tcgaaggctg
     1861 ggggaccaga gtacctgtat aactttgttg aggcgaaagc gattggggaa cgcctgtgag
     1921 caccggcagt ctggcagcga cgagcgcggc ccaaacgatg agctcgcgtg ccgcgctggc
     1981 cctcaatccc gtcttccgaa aaggaatcct gctggcgacg agcaaccctg tggtgtcgcg
     2041 ctcggtgcat cggtatggca tgcggctcgg ggcatcgcgg ttcgtggcgg gcgaaacgct
     2101 cgatgagtgc attccggtcc tgaagggcct gaacgcgaag gggctacgca tcaacacgac
     2161 gatccttggt gaggatgtgg gcgacgaggc cgctgcggcc gcagtagctg atgcctatgt
     2221 cgaagtgctc gaccggctgg cggtcgaaca gatcaatggc aacgtcgcgc tcaagctcac
     2281 gcacctggga ctcaatcttg gcgaggaaat cgcctaccag aatgttgcgc gcctcgtcga
     2341 atacgccgac acgctgaaca acttcatccg gatcgacatg gaggagtcgg cgtgggtcga
     2401 cgcgacgctg cgcatctacc gacggctgcg tgagcagggc cacgacaatg tcggaacggt
     2461 cctccaggcc tacctctacc gcaccgagca ggacctgacg gatctgctgc cgctggagcc
     2521 caatctcagg ctggtcaagg gggcgtatct ggagcccgac agcatcgcgt tcgccaggaa
     2581 atccgatgtc gatgcgaact acctgcggct cgccgagcgc atgctcgccg gtggcgggtt
     2641 cacggcgatt gccacgcagg acgagcgcat catcgagcac gtgatcaatc tcacggaacg
     2701 cgaggcgatc ccgcgtgatc gattcgagtt ccagatgatg tatggcattc ggccgcaact
     2761 gcaactcgac ctcgtccagc aggggtatcg ggtgctggtc gcgacgccat acgggccaga
     2821 ctggtatcac tacctgatgc gccgtctggc cgaacgcccc gccaacctgc tgttctttgc
     2881 caggaacctg gttcgccggt agcgagcgtg caagagcttt cgagttcaac tgtagcgggt
     2941 cttagagcgg ttgtcagatc agatgggctg ggagcgggta gccgcagtgg gcgaaccagc
     3001 cccgggcgtc ggcggcggtg atggtggcca gcgccaccgt gagatcgtgc tcgttccgac
     3061 gccgaagatg cgggcggcct cggcctgact gaggccggcg tcggtggcca ccagcagccg
     3121 ctcgcggagg tcgaccgagt actgcttcat gtcgccagcc tcgcacgggt ccaactattc
     3181 cgctacccgc tctaagacac ggacgacata gttggatggg cgttcgacaa cttgccggac
     3241 ggaatgtcag tgggaggtcc gtacccagtg ctggtgtcgc gggagacaga aatacgaatc
     3301 ctgtcgaatc aatcatcatc gccggggacc aagtcgccaa tagaagcggg tgcgcgcgtg
     3361 gatggtggtc tgaggggtac gccgcagttg gcagcggacc accagcacct cggccaagtc
     3421 ttctagcttg gggcaggctc ggttattacc agcgcgtcca gcagctccca cagccgctcc
     3481 accggctaca gcctagggga tgccgagggg tggctccagt tcggttctat cggagggcca
     3541 gggtagcaca cgagccggcc ccatcccaga ccagcactgc cgggtggttc gagtcagtac
     3601 ccgcagcact gctaaaggct ggcagcacca actcatggtc accgccgctg ctggcagcag
     3661 acaccaccgg ctctgcccag cgg
//
LOCUS       DASYWL010000291         3229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_239586, whole genome shotgun sequence.
ACCESSION   DASYWL010000291 DASYWL010000000
VERSION     DASYWL010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239586"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VGR22_10330"
     CDS             complement(<1..522)
                     /locus_tag="VGR22_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129003.1"
                     /translation="MNQQAEQNDHGMNPDEMMRQHRVSTLWMYFANIALGLWLISSAA
                     AFDYQDTALVWSDILSGTLIVLLGVVALFPKGDFWGRWGICLIGIWLLFAPIVFWAPT
                     AFVYANDSLIGGLVISLAILAPMMPGKAHHMAMMQPGPDIPPGWTYNPSSWWQRGPVI
                     ALGLVSFLISRYMA"
     gene            complement(640..885)
                     /locus_tag="VGR22_10335"
     CDS             complement(640..885)
                     /locus_tag="VGR22_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129004.1"
                     /translation="MDELFQRRRHHHLSMSFSNIALGLWLLSSPALFDYQNTALARSE
                     ALNGVAVMVLGILAPSKCGDGIDCARIAYIGRHSGGC"
     gene            complement(1077..2279)
                     /locus_tag="VGR22_10340"
     CDS             complement(1077..2279)
                     /locus_tag="VGR22_10340"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2129005.1"
                     /translation="MSTKRTGFSLLVLVTFLAFVSIGFPDAVLGVAWPSIRATFDRAL
                     SDVGFILVSSGSGYFLSGVLAGAAIERLGIGRLLVLSTTAVAVGLFSYAATPNFWLLL
                     GAAVLIGFGSGAIDTGLNVYASEHFSVTVMNWLHAFFGIGAMLGPFIMASVLAAGGSW
                     RIGYIIVASAIAAMAAVFVVTARQWDGDAHHVDAEPAARISARLVVRMPLVWVQVVLF
                     FVMPGLEASAGLWTATMMLGRFDASAGEAGLWAGIFWGAMALGRLVLAPLSGDLNPAR
                     LVQLGTIGVLLGSILMIPDQQWVFQAGLIVFGLAMAPLFPTLMSLTPRRFGSQVAMHA
                     IGFQVSAATAGIIGVPVIAGYLAERTTLSTIPWVMSIGAVVVIALETLLRTRADRQSP
                     ATATPTPA"
     gene            complement(2357..>3229)
                     /gene="aroF"
                     /locus_tag="VGR22_10345"
     CDS             complement(2357..>3229)
                     /gene="aroF"
                     /locus_tag="VGR22_10345"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV2129006.1"
                     /translation="GVDSVVRITKKFKLTGWDFHPQKTVIDLGDGVTVGGNEISIMAG
                     PCSVESEAQTLETAKAVAASGATILRGGAFKPRTSPYEFRGLGERGLEILAQAREATG
                     LKIITEVMTPADVETVGQYTDIFQIGARNSQNYLLLEEVGKAKKPVMLKRGLSMLLEE
                     WLLAAEYVMAQGNPDVMLCERGIRTFETMTRNTLDVAAVPVLQSLSHLPVIIDPSHAA
                     GKRAYVEALGKAGIAAGADGLIIEVHPNPDQAMSDGAQSVDFAQFRAMVPKLARVAEA
                     VERVAPRVAVPTPV"
BASE COUNT          583 a         1063 c         1089 g          494 t
ORIGIN      
        1 cgccatgtag cgagagatca ggaagctgac gagaccgagc gcgatgaccg ggccgcgctg
       61 ccaccagctg gaggggttgt aggtccagcc cggcgggata tccggcccgg gctgcatcat
      121 cgccatgtgg tgagccttgc ctggcatcat cggggcgagg atggcgagcg agatgacgag
      181 tccgccgatg agcgagtcgt tggcgtagac gaaggcggtc ggcgcccaga agacgatcgg
      241 ggcaaacagg agccagatgc ctatcagaca gataccccac cggccccaga aatcgccctt
      301 ggggaacagg gcgacgacgc cgagcagcac gatcagcgtg ccgctcagga tgtcactcca
      361 gacgagcgcg gtatcctggt agtcgaaggc agcggcgctg ctgatcagcc agagcccgag
      421 cgcgatgttg gcgaagtaca tccacagggt gctgacgcgg tgttgccgca tcatctcgtc
      481 ggggttcatt ccgtgatcgt tttgctcggc ctgctgattc atcgggtaca ccctccctgg
      541 cttcgctgtg cacgctggaa gccggcgcat tgccccgtca tgcaacagcg gcagcggccg
      601 gcacgtgtac gtggatccac tggccgagta ccgcagcgtt cagcatccac cggagtggcg
      661 cccgatgtag gctatccggg cacaatcaat gccatcaccg cacttggatg gcgccaggat
      721 gcccagcacc atgacggcta ccccgttcag agcctcgctt cgggcgaggg cggtgttctg
      781 gtagtcgaac aatgccggac tggaaagcag ccacagcccg agcgcgatat tggagaagga
      841 catcgagagg tgatgatgcc tgcgccgctg gaacagttcg tccacgcagg ggggtaacga
      901 accatacctc cgccctgtcc attcgcatcg gaagcccgga tctttgctct cttctggtcg
      961 ggctggtgcg acatggggca cctctcccct gcgatgcatg acgggaatcg ttgaggagag
     1021 gtgtcgcaat ggtggtgcca gtggtggcgg ggagcggcag gtatccccgc cgtcgctcag
     1081 gcgggcgtgg gcgtcgctgt tgcgggagac tgacgatcgg cgcgggtgcg caggagggtc
     1141 tccagcgcga tcacgacgac ggcgcctatg gacatcaccc atgggatcgt ggacaacgtc
     1201 gttcgctcgg cgaggtatcc cgcgatgacg ggaaccccga tgatcccggc ggtggcagcg
     1261 ctgacctgaa agccgattgc gtgcatcgcg acctgcgagc cgaagcgcct cggggtgagc
     1321 gacatcaggg tggggaagag cggggccatc gccaggccga agacgatcag gccggcctgg
     1381 aacacccact gctggtccgg aatcatcagg atcgacccga ggagcacacc gatcgtgccg
     1441 agctgtacca gccgtgccgg gttgagatcg cccgacagcg gcgcgagcac gaggcggccg
     1501 agggccattg cgccccagaa gatacccgcc cagagcccgg cttcgccggc actcgcatcg
     1561 aaacgaccca gcatcatcgt cgcggtccac agcccggcgc ttgcctccag accaggcatc
     1621 acgaagaaga gcaccacctg cacccagacc agtggcattc gcacgaccag ccgcgcggag
     1681 atacgcgcgg ctggctccgc atcgacgtgg tgggcgtcgc cgtcccactg ccgagcggtc
     1741 actacgaaga cagccgccat cgccgcgatc gcgctggcga caatgatgta gccgatccgc
     1801 cagctgccgc cagcggcgag cacactcgcc atgatgaacg gacccagcat ggcgccgatg
     1861 ccgaagaatg cgtgaagcca gttcatcacg gtgacggaga agtgctcgga cgcgtagacg
     1921 ttgagtcctg tgtcaatggc gccggagccg aacccgatga ggacggcggc gccgagcagg
     1981 agccagaagt tcggtgtggc ggcgtaggag aagagcccta cggcgacggc agtggtgctg
     2041 agcaccagca ggcggccgat gccgagacgc tcgattgctg ccccggcaag gacgccggag
     2101 aggaagtacc cggatcccga cgagacgagg atgaagccga cgtccgacag cgcgcggtcg
     2161 aaggtcgcac gtatcgaggg ccacgcgacc cccagcaccg cgtcgggaaa gccgatgctg
     2221 acaaaggcga ggaaggtgac cagcaccagc aggctaaagc cggtccgttt cgtgctcatg
     2281 gttcctgccg tctaacgaaa accgccgggg gcagccccgg cggtggtgac gacgtctgtc
     2341 ctaggcggtg gctcggctag acaggggtcg gcaccgcgac gcggggggcg actcgctcga
     2401 ccgcctcggc cacccgcgcc agcttcggca ccatcgcccg gaactgggcg aagtccaccg
     2461 actgcgcccc gtccgacatc gcctggtccg ggttcgggtg cacctcgatg atcagcccgt
     2521 ccgccccggc cgcgatcccc gccttgccca gcgcctcgac gtaggcccgc ttgccggcgg
     2581 cgtgggacgg gtcgatgatc accggcaggt gcgacaggct ctgcagcacc ggcaccgccg
     2641 ccacatcgag cgtgttgcgc gtcatcgtct cgaaggtgcg aatcccccgc tcgcacagca
     2701 tcacgtcggg gttgccctgg gccatcacgt actccgcggc cagcagccac tcctccagca
     2761 gcatcgacag cccccgcttg agcatcaccg gctttttcgc cttgccgacc tcctccagca
     2821 gcaggtagtt ctggctgttg cgcgccccga tctggaagat gtcggtgtac tgccccaccg
     2881 tctccacatc ggccggggtc atcacctcgg tgatgatctt cagccccgtg gcctcgcgcg
     2941 cctgggccag gatctccagg ccgcgctcac ccagcccccg gaactcgtag ggcgaggtgc
     3001 gcggcttgaa ggctccgccg cggaggatgg tggcgccgct ggccgcaacc gccttggccg
     3061 tctccagcgt ctgggcctcg ctctcgaccg agcacggccc ggccatgatc gagatctcgt
     3121 tcccgccgac ggtgacgcca tcgccgaggt cgatgacggt tttctggggg tggaagtccc
     3181 agccggtgag cttgaacttc ttggtgatgc gcaccacgct gtcgacgcc
//
LOCUS       DASYWL010000292         1881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_202596, whole genome shotgun sequence.
ACCESSION   DASYWL010000292 DASYWL010000000
VERSION     DASYWL010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202596"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VGR22_10350"
     CDS             <1..367
                     /locus_tag="VGR22_10350"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEV2129007.1"
                     /translation="AMDVQVTSPVYENATFGEVPLLDAIATFDEANEELTIFAVNRSQ
                     DGPLPIEGDLRALAGSRVLEHLVLTHEDPKAVNTADAPETVVPHAKGDAVHNGGALTA
                     TLPALSWNVIRLAEGDARG"
     gene            695..>1881
                     /locus_tag="VGR22_10355"
     CDS             695..>1881
                     /locus_tag="VGR22_10355"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2129008.1"
                     /translation="MVRLLDDPSVRLLTLTGPGGVGKTRLAIEVAHRIRADFADGACF
                     VPLAPVGDPTLVLPTIAHVLGIPTHPTQPLWDTLVGTLRDGHLLLVLDNFEHLLIEPP
                     AWLAELAATCPRLTVLVTSQVALHIGGEQRFEVPPLRLSSRSGERAGFSEAMALFEQR
                     AQAVRPDFAVNSKNQAAVERICHEVDGLPLAIELAASRILFLHPAEIAAHLSHRLRLL
                     TGGQRDMPPRLQSMHSAITWSHDLLDADGRRLFRRLAVFVDGFTLDAAEAVCAGDEGG
                     RFDVLGGLASLLDHSLIRRDDESDHARFMMLEVIREFGVEQLEASGEINVIRDRHAGW
                     CLAISRRVAPGPQHRSQPELSEIARFEVPPLRLSSRSGERAGFSEAMALFEQRAQAVR
                     PDFA"
BASE COUNT          303 a          616 c          621 g          341 t
ORIGIN      
        1 ggcgatggat gtgcaggtca cctcgccggt ctacgagaac gcgaccttcg gcgaagtgcc
       61 gctgctcgac gcgatcgcga cgttcgacga ggcgaacgag gagttgacga tcttcgccgt
      121 gaaccgcagt caggacggcc cgctgccgat cgagggtgat ctccgcgcgc tggcaggctc
      181 ccgcgtcctt gagcacctgg tcctgacgca tgaggatccg aaggcggtca acaccgccga
      241 cgcgccggag accgtcgtgc cccacgccaa gggtgatgca gttcacaacg gcggcgccct
      301 caccgcgacg cttccggcgc tctcctggaa cgtcatccgc cttgccgagg gcgatgcccg
      361 agggtgagtg gtggcgccgg catgatgtcc tcagagagtg aactgggctg aacccgatga
      421 tcgtgagagg gctccagccg cagatctccc cctgcacggc acgacgcgct tcctctcgca
      481 cctagcccta acttgtcatt tacgtaacag atcatcagtt gtaagatacc ggtgaggtct
      541 gcccccgaca ccgagggatt ccggcgtggc cacgcaacgt gtacctttcg actttaccga
      601 cctcaatatg acctctgagc ccggcgagcg aatcgatagc gggcttcctt agccgctcac
      661 gtcctttctc ggccgcgatg aggtgatctc gcgggtggtt cggctgcttg acgatccgtc
      721 ggtccgactg ctgacgctga ccggaccggg tggggtcggc aagacccgcc ttgccattga
      781 ggtggcacat cgaatcaggg cagactttgc cgacggagcc tgcttcgtgc cgctggcccc
      841 ggttggagat ccgacgctcg ttctcccgac catcgcgcac gtgctgggca ttcccacgca
      901 tccgacccaa ccgctgtggg ataccctcgt cggcacgctg cgggacggcc acctgctgct
      961 cgtgctcgac aacttcgagc atctgctgat tgagccaccg gcatggctcg ccgagctggc
     1021 cgccacctgt ccgcgcctca ctgtgctcgt cacgtcgcag gtagccctgc acatcggcgg
     1081 cgagcagcgg ttcgaggtgc ccccgttgcg gctgtcgagc cgcagcgggg agagggccgg
     1141 gttctccgaa gccatggcat tgttcgagca acgcgcgcag gcggtcaggc cagacttcgc
     1201 ggtgaacagc aagaaccagg cggctgtcga gcgtatctgt cacgaggtgg acggtctgcc
     1261 gctggcgatc gagctggcgg cgtcgcgaat cctctttctt catccggccg agattgctgc
     1321 ccacctgtcg catcggctgc gcctgctcac cggcgggcag cgcgacatgc cgccgcgtct
     1381 ccagtcgatg cacagcgcca tcacttggag ccatgatctg ctcgatgccg acgggcgccg
     1441 actctttcgg cgacttgccg tcttcgtcga cgggtttacc ctcgatgcgg cggaagctgt
     1501 ctgtgccggc gacgagggcg gcaggttcga tgtgctcggg ggactggcat cgctgctgga
     1561 tcacagcctc attcgacgcg acgacgagag cgaccacgcg cgattcatga tgctggaggt
     1621 gatccgggag ttcggcgtcg agcagctgga agcgagcggt gagatcaatg tcatccgcga
     1681 tcgccatgcc ggctggtgcc tcgccatttc caggcgagtg gcaccggggc cgcagcaccg
     1741 gagccagccg gaactttccg agatcgcgcg gttcgaggtg cccccgttgc ggctgtcgag
     1801 ccgcagcggg gagagggccg ggttctccga agccatggca ttgttcgagc aacgcgcgca
     1861 ggcggtcagg ccagacttcg c
//
LOCUS       DASYWL010000293         3168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_202605, whole genome shotgun sequence.
ACCESSION   DASYWL010000293 DASYWL010000000
VERSION     DASYWL010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202605"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(249..2603)
                     /locus_tag="VGR22_10360"
     CDS             complement(249..2603)
                     /locus_tag="VGR22_10360"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEV2129009.1"
                     /translation="MATQSVPVDFTDLNMTSEPGERIDSGLPSPLTSFLGRDEVISRV
                     VRLLDDPSVRLLTLTGPGGVGKTRLAIEVAHRIRADFADGACFVPLAPVGDPTLVLPA
                     IAHVLGIPTHPTQPLWDTLVDTLRDGHLLLLLDNFEHLLFEPPAWLAELIATCPRLTV
                     LVTSQVALHVGGEQRFEVPPLRLSSRSGERAGFSEAVALFEQRAQAVRPDFAVNSKNQ
                     AAVERICRAVDGLPLAIELAASRILFLHPAEIAAHLSHRLRLLTGGQRDMPPRLQSMH
                     NAMTWSHDLLDSNERRLFRRLAVFVDGFTLDAAEEVCAGGEGTAFDVLGGLASLLDHS
                     LIRRDDESDHARFMMLEVIREFGVEQLEASGEIDVIRDRHADQCLAIASRVAPGPQHR
                     SQPELSEIARFEADYGNVRAALAWLDESGRHGDLAQLAMYLRSFWYLTDRYEEALRWL
                     ERLLQLNPEPSIDVLCTAGQMAQLLGTPDAAHYLERALARAEPSGDAYQEAEARFHLA
                     IHLEDSGAYAQAERLFVEAQERYERAGNALGMRQSDYHLGVVAFGLGRLPQAKRRLEE
                     VIVAAQAAGDPLLPVWSYNYLVLIACEERNAPRAVELLRRQAQVLAAPALRHHVPDFL
                     AAAGVVAVARCQFDRAGRLLGASGRSPRALMLPERAAFERAFDTAREHLGEAELEREF
                     AIGRHLTEAEIGGELTDLLDGDDTPGSQANRTSAPRDVALDLSSRQLDVLRLVADGLT
                     NQEIADALFISPRTVATHVDHILAKLDVRSRTAAVACAIRNGLA"
     gene            complement(2802..>3168)
                     /locus_tag="VGR22_10365"
     CDS             complement(2802..>3168)
                     /locus_tag="VGR22_10365"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEV2129010.1"
                     /translation="AMDVQVTSPVYENATFGEVPLLDAIATFDEANEELTIFAVNRSQ
                     DGPLPIEGDLRALAGSRVLEHLVLTHEDPKAANTADAPETVVPHAKGDAVHNGGTLTA
                     TLPTLSWNVIRLAEDDARG"
BASE COUNT          573 a         1040 c         1044 g          511 t
ORIGIN      
        1 cgaaccggct cgccggaggg ggtgtctggg catgaacggc atcctgtgct gcacaccagc
       61 gattgcacag gctccagtat cgacgatcgc actgctggtc gcgagcagat catctgtctt
      121 cccaccacca cgagcgacca aaatcgaata gcaccgctca atgctccgac tgtggacggc
      181 cagagtgtag ctcgccggtc acacccgcct gagaggggcc gtgagccctg tgaccgagaa
      241 ggccgagctc aggcgagtcc gttgcggatg gcacatgcga cggccgccgt gcgcgatcgg
      301 acatcgagct tggcgaggat gtgatcgacg tgcgtggcaa ccgttcgtgg gctgatgaag
      361 agcgcgtcgg cgatctcctg attggtcagg ccatccgcca ccagccgcag cacgtccaac
      421 tgccgggagg agaggtccaa cgcgacgtcg cgaggcgctg atgtccggtt cgcttgtgag
      481 cctggggtgt cgtcaccgtc gaggaggtcc gtcagttcgc cgccaatctc agcctccgtc
      541 aggtgacggc cgatcgcgaa ctcgcgctcg agctccgcct cccccagatg ctcccgtgcg
      601 gtatcgaacg cgcgctcgaa tgccgcgcgc tcgggcagca tgagcgcacg tggactccga
      661 cccgaggcgc ccagcaagcg cccggcgcga tcgaactggc agcgagcgac tgctaccaca
      721 cccgctgcgg cgagaaagtc cggcacgtga tgccgcagtg ccggcgcggc gagcacttgc
      781 gcctgccgcc ggagcaactc gacggcacgg ggagcgtttc gctcctcgca ggcaatgagc
      841 accagatagt tgtatgacca gacggggagc agcggatcgc cggccgcctg cgccgcaaca
      901 atgacctcct cgagtcgacg ctttgcttgg gggaggcgtc ccagcccgaa ggcgacaaca
      961 ccgaggtggt aatcgctctg tcgcatcccc agcgcattgc cggcccgctc gtatcgctcc
     1021 tgcgcctcca cgaacaagcg ttctgcctgt gcgtaggccc ctgagtcctc aaggtgaatg
     1081 gcgagatgga agcgagcttc cgcctcctga taggcatccc ccgatggttc cgcgcgcgcg
     1141 agcgcccgct ccagatagtg agccgcatct ggagtgccga gcagttgtgc catctggccg
     1201 gcggtgcaca gcacgtcgat ggacggctcg gggttgagct ggagcagccg ctccagccag
     1261 cgcaacgcct cctcgtaccg atccgtgagg taccagaacg accgcaggta catggccaat
     1321 tgcgcaaggt caccatgccg tccggactcg tcgagccatg ccagcgcggc gcgcacgttg
     1381 ccgtagtctg cctcgaaccg cgcgatctcg gaaagttccg gctggctccg gtgctgcggc
     1441 cccggtgcca ctcggctggc aatggcgagg cactggtcgg catggcggtc gcggatgaca
     1501 tcgatctcgc cactcgcttc cagctgctcg acaccgaact cccggatcac ctccagcatc
     1561 atgaatcgcg cgtggtcgct ctcgtcgtcg cgtcgaatga ggctgtgatc cagcagcgat
     1621 gccagcccgc cgagcacatc gaaggcagtc ccctcaccac cggcacagac ctcttccgct
     1681 gcatcgaggg tgaacccatc gacgaagacg gcaagccgcc ggaagagccg gcgttcgttg
     1741 gaatcgagca gatcgtggct ccaggtcatg gcattgtgca tcgactggag ccgcggcggc
     1801 atgtcgcgtt gcccaccggt gagcaggcgc agccgatgcg acaggtgggc ggcaatctcg
     1861 gccggatgaa gaaagaggat tcgcgacgcc gccagctcga ttgccagtgg caggccgtcc
     1921 accgcacgac agatgcgctc gacggcagcc tggttcttgc tgttcaccgc gaagtctggc
     1981 ctgaccgcct gcgcacgttg ctcgaacagc gccacggctt cggagaaccc ggccctctcc
     2041 ccgctgcggc tcgacagccg caacgggggc acctcaaacc gctgctcgcc gccgacgtgc
     2101 agggccacct gcgacgtgac gagcacagtg aggcgcggac aggtggcgat cagctcggcg
     2161 agccaagcgg gcggctcgaa cagaagatgc tcgaaattgt cgagcaagag cagcaggtgg
     2221 ccgtcccgca gcgtgtcgac gagggtatcc cacagcggtt gggtcggatg cgtgggaatg
     2281 cccagcacgt gcgcgatggc cgggagaacg agcgtcggat ctccgaccgg agccagcggc
     2341 acgaagcagg ctccgtcggc aaagtctgcc ctgatccgat gtgccacctc aatggcaagg
     2401 cgggtcttgc cgaccccacc cggtccggtc agcgtcagca gtcgaaccga cggatcgtca
     2461 agcagccgaa ccacccgcga gatcacctca tcgcggccga gaaaggacgt gagcggcgaa
     2521 ggaagcccgc tatcgattcg ctcgccgggc tcagaggtca tattgaggtc ggtgaagtcg
     2581 acaggtacag actgcgtggc catgccaaaa tccctcggtg tcggaggcag tcctcgccgg
     2641 tatcttacta ctgatgatct gttacgtaaa tgacaagatg gggctaggtg cgaggggaag
     2701 cgcgtcgtgc cgtgcagggg gagatctgcg gctggggccg tctcacgatc attgggttca
     2761 gcccagttca ctcgccgagg acatcatgcc gacgccacca ctcaccctcg ggcatcgtcc
     2821 tcggcgagcc gaatgacgtt ccaggagagc gtcggaagcg tcgcggtgag ggtgccgccg
     2881 ttgtgaactg cgtcaccctt ggcgtggggc acgacggtct ccggcgcatc ggcggtgttg
     2941 gccgccttcg gatcctcatg cgtcaggacc aggtgctcaa ggacgcggga tcctgccagc
     3001 gcgcggagat caccctcgat cggcagtggg ccgtcctgac tgcggttcac ggcgaagatc
     3061 gtcaactcct cgttcgcctc gtcgaacgtc gcgatcgcgt cgagcagcgg cacttcgccg
     3121 aaggtcgcgt tctcgtagac cggcgaggtg acctgcacat ccatcgcc
//
LOCUS       DASYWL010000294         5515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_484508, whole genome shotgun sequence.
ACCESSION   DASYWL010000294 DASYWL010000000
VERSION     DASYWL010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5515
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484508"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..618)
                     /locus_tag="VGR22_10370"
     CDS             complement(22..618)
                     /locus_tag="VGR22_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129011.1"
                     /translation="MQVRAAMPSGMFAIVLVLIATMVVGVVTVTAQDAATPAVPDEQL
                     PYQASINAGSCDALGDVVFELNEVTQADDAGTPAAPVNGAAVASPAVEGGEIGGDVAE
                     STTEVDASLEELVAEERVIVVYQGTDGTDAYLACGEITGSPTSGALQVELLEDSESGV
                     SGTAMLLTGGEDDDSTTVTILITEGEIETRDQEATPAS"
     gene            complement(1530..2009)
                     /locus_tag="VGR22_10375"
     CDS             complement(1530..2009)
                     /locus_tag="VGR22_10375"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEV2129012.1"
                     /translation="MSQQTFDRRQLLGASVIIGAAGTALGPRAVLGHEGESHEGTPAA
                     ATPGATPGATPSAGATHIVEMTGELRFVPEYLTLRAGDTVVWRNVSTAPHTSTCDEEL
                     VKQRENVARPADAEPWHSGPLGEGEEYSRAFPVAGEYTYFCKPHEAAGMVGHLTVEE"
     gene            complement(2169..2726)
                     /locus_tag="VGR22_10380"
     CDS             complement(2169..2726)
                     /locus_tag="VGR22_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129013.1"
                     /translation="MRRNQFAPLAALVLALALVLGAWTPGTAHAVLTAHLHEGTCADS
                     GEQPVDLGELGLATSLLDSSQATPAGPYDLVGAGGAFPTVLGHASIDRSLDDLLASPH
                     VIDIHVVNDEEGTDVILACGAVGGVLANGELVFGLQSLPSEGIDTTGIAWLQANDDGT
                     TTVRLIVAQGLAPGRVGAASSTPAS"
     gene            complement(2829..3308)
                     /locus_tag="VGR22_10385"
     CDS             complement(2829..3308)
                     /locus_tag="VGR22_10385"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEV2129014.1"
                     /translation="MSQRTFDRRKLLGAGVIVGATGTALGFRAVLANYHEDEEEATET
                     VSIETQAVGSTPAAATPGASPEVAGMVQVELTEFEIRMPRELPAGQTTFEVTNVGSIE
                     HNFEVEGQGIEREFEQNLQPSETKTMTVDLEPGTYEVYCPVGNHAEQGMRLELTVTD"
     gene            complement(3688..4449)
                     /locus_tag="VGR22_10390"
     CDS             complement(3688..4449)
                     /locus_tag="VGR22_10390"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEV2129015.1"
                     /translation="MSDGTAHRPSSRPDPDDAVAAYALGVLDPEERQEFEAHLAGCPT
                     CRAALSRHEEVVGAVGSSVTPVAPRPGLRDALLAEIRAPAPPAALPAPRRLRVLAIGS
                     GIAASIALVSVAVLSLLLARALDERDEARHSAQEIAEYISDGGTLSALLPAAEAPPVA
                     APGHGSLVVAPDQSQAMLVVHDLTPSGDDRRYIAWAERDGERVELGVLPVNDEGVGWL
                     LLSGREPMSAYETVGITRYSEQAPEGEPFLVAPIE"
     gene            complement(4446..5030)
                     /locus_tag="VGR22_10395"
     CDS             complement(4446..5030)
                     /locus_tag="VGR22_10395"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2129016.1"
                     /translation="MRRVRGHDEDAFATLYDRYAAQVNGLALSILRNPALSEEATHDV
                     FLRLWQEPESYDSSRGSFAGWLLRAVRNRAIDLLRRRREHSIGGMEVDLASWIPDPDP
                     GPEEQTMIQQHRQEVRQALDELVPDQRHLLELAYFTGLSQRQIADHLERPLGTVKSQI
                     RVAMRRLADRMGEPGQGSADSATRIPPSRKRDLE"
BASE COUNT          915 a         1818 c         1811 g          971 t
ORIGIN      
        1 gttcgggcct tcgcctcggg atcaggaggc gggtgtcgct tcctgatccc gagtctcgat
       61 ttctccctcg gtgatgagaa tagtgacggt ggtgctgtca tcatcttcac cgccagtcag
      121 cagcatggcg gtgcccgata cacccgactc gctgtcttcc aacaactcca cctgcagcgc
      181 cccgctggtg ggcgagcccg tgatttcacc gcaggcgagg taagcatccg tgccatcggt
      241 gccttggtag acgacgatca cccgctcctc ggcgacgagc tcctcaagtg aggcgtcgac
      301 ctccgtggta ctttccgcaa catcgccacc gatctcgccc ccctcgacag cgggagatgc
      361 aaccgcagca ccgttgacgg gggccgccgg ggttcctgcg tcatcggcct gtgtgacctc
      421 attgagctcg aagacgacgt cgcccaatgc gtcacaactg cccgcgttga tgctcgcctg
      481 gtacggcaat tgctcgtcgg ggacggccgg ggtcgctgca tcctgtgccg tcacagtcac
      541 cacccctacc accatcgtgg cgataagcac gagaactatc gcgaacattc cagacggcat
      601 cgctgctcga acctgcatgg ctgctactct ccctcagtaa acaaacacct tgcggcatca
      661 gtggccgcgt tgcaatcacc gcactagccg tgccatgacc aactgcgcgt cgagtgcctg
      721 acatgcaagt cgtagatcgg actgcggttg agagcggtcg cattaccgcg gcatgctgcc
      781 tcgccgatct agaggatggg tggctgctcg gcgaaggggc gctattcctc atgagaacaa
      841 ccgtgagccg ttgagcacgg ctggtcggca gggaagccac tacacggcca ggagcagttc
      901 cagcaagatc atcccaatgt gcgccaagga gttgacggag ccgctccgtt agcagtcgca
      961 ggctttcgga ctggtgctgg gcggtgtgcc acacctcgta cactgcccgc tcgaggacca
     1021 gcgcgcccag catggcgatg cagtcgtccc tcgtttccgg caacatggcg tccccctccg
     1081 cctcattatt gatgtacccc gttaccagcg cggcgctggc gtgccggaac cacgtgtcga
     1141 gcagcgcgcc gacgacgccg agcaccacca ccccgtctgc cacggcaatc gccccactca
     1201 ccgagtcctc gaggctgtac agccagagtt gcgcgccgct gcccagcgca ggcgtggagg
     1261 acgccaccaa tgccggcgcc tcacctgggc cggtccgatg cctgctccgc gtccgcgctt
     1321 gcggttggtg tcgcgccatg attctcgccc tcgtgagcca ctcacacggt gctgctgaag
     1381 atcaggctca acagtgctga cgcgattccg acgacacgca gtaggttccg gatctgttgc
     1441 acatggtaac ctcctgtgat tcctgtctgg acaggttctc tgatgattgg ggaggacgag
     1501 cagcggccct gcgtgcaggg cacggatgct tactcctcaa cggtcaaatg gccgaccatg
     1561 ccggccgctt cgtgaggctt gcagaagtac gtgtactcgc ccgccactgg gaaggctcgc
     1621 gagtactcct caccctcgcc gagaggaccg gagtgccatg gttcggcatc cgcgggccgt
     1681 gcgacattct cccgttgttt caccagctcc tcgtcacagg tcgatgtgtg cggggccgtg
     1741 ctgacgttgc gccacaccac ggtatccccc gccctgagcg tgaggtactc gggcacgaac
     1801 cgcagttcac cggtcatctc aacgatgtgc gtggctcctg cagagggtgt cgcgccgggc
     1861 gtcgctcccg gcgtggctgc ggctggcgta ccctcgtggc tctcgccttc gtggccgagc
     1921 accgcgcggg ggccgagggc agtgccggcc gcgccaataa tcacactcgc tccgagcaac
     1981 tgccgtcgat cgaacgtttg ttgagacatt ttgatcctcc tgtcgttaca caaggcaaat
     2041 gtttcttccc tgaccactgc ttctccatga agtatcaagg ctctctgccg gcacggcgga
     2101 accggcacct tatccgaagc accgggagtt gaggagccct tgatcgggcc cgtgcgtcga
     2161 gcgtggggct acgaggccgg ggtgctgctg gcagccccga ccctgccggg ggcgagcccc
     2221 tgtgcgacga tgaggcggac ggtcgtcgtt ccatcatcgt tcgcctgcag ccaggcgatc
     2281 cccgtcgtgt cgatcccttc gctcgggagc gattgcagcc cgaagacgag ctcgccgttg
     2341 gccagcacgc ccccgacggc gccacaggcg aggatgacgt cggtgccttc ctcgtcattc
     2401 accacatgga tgtcgatcac atgcggagac gcgaggagat catcgagcga tcggtcgatg
     2461 ctggcgtggc cgagcaccgt tgggaacgcc ccgcccgcgc ccaccagatc gtacggcccc
     2521 gcaggcgtgg cctgcgacga gtcgaggagc gaggtggcga gtccgagctc gcccagatcg
     2581 accggctgct cgccggagtc ggcacatgtg ccctcgtgca ggtgggcggt caggacggca
     2641 tgggcggtcc cgggcgtcca ggcaccgaga acgagcgcca gcgccaggac gagtgcagcg
     2701 aggggggcga actggttgcg cctcatgatc gagtcccttt cccggcaggc atgcagctgg
     2761 tggagcgcgg cccgagccaa acccctggct caggccacgg caccatgacg tccaccatgt
     2821 tctcggggtc agtcagtaac tgtcagctcc aaccgcatgc cctgctcggc gtggttgccc
     2881 accgggcagt agacctcgta ggtgccgggc tccaggtcca ccgtcatcgt cttggtctcg
     2941 ctcggctgca ggttctgctc gaactcgcgc tcgatgccct ggccctcgac ctcgaagttg
     3001 tgttcgatgg acccgacgtt ggtcacctcg aacgtcgtct ggccggccgg cagctcgcgg
     3061 ggcatgcgga tctcgaactc cgtcagctcc acctgcacca ttcccgccac ctcagggctg
     3121 gcgcccgggg tcgcggctgc tggggtgctg ccaacagctt gggtctcgat gctgaccgtc
     3181 tcggtcgctt cctcctcgtc ctcgtggtag ttggcgagca ccgcccggaa accgagggcc
     3241 gtgccggtcg cgccaacaat cacgccggca ccgagcagct tgcgtcgatc gaatgttcgc
     3301 tgagacatcc tgatcctcct catctgaggc atcggcggca tcatcgtggc cgcctaccgt
     3361 gctcatcgcg ccatcgggaa ccacggtccg gtggcgccac gcaccctgtg cgtaccctcc
     3421 ctacgcgcga gagacgggtt cggatgagga tgccgtcgct aggggcgctg ctggcaggtt
     3481 gttcgtccgg tcggaggact ggcgaatcgg aggtcgattc ggtgccgaac tcgggacggg
     3541 tcgataacgg gcaggaggct gctgactgca agggcggcgt ccaggaggac aggtgccgtc
     3601 gctgatggcg tgcctccgcg ccaacagcag accaacgaag atcgcgtatc ccgcgacgag
     3661 gccaggcgtc atgccctgat tgtggaccta ctcgatgggc gcgaccagga acggctcacc
     3721 ctcgggagcc tgctcggaat agcgggtgat cccgaccgtc tcgtaggccg acatcggctc
     3781 tcgaccggag agcaggagcc agccgacgcc ctcgtcgttg acgggcagta cccccaactc
     3841 cactcgctcg ccatcgcgct cagcccaggc aatgtagcgc cggtcatcac cggagggagt
     3901 gaggtcgtgg acgacgagca tcgcctgcga ctggtcagga gcgacgacga gagagccgtg
     3961 gccgggcgcg gccacgggcg gcgcctcggc ggccgggagt agcgcggaga gcgttccccc
     4021 gtcgctgatg tattcggcaa tttcctgcgc gctatggcgg gcctcgtcgc gctcatccag
     4081 cgcacgggcg aggaggaggc tgaggacggc gacggagacc agcgcgatcg aggctgcaat
     4141 ccccgagccg atcgcgagca cccgaaggcg gcgcggggcg ggaagcgccg ccggcggagc
     4201 gggagcgcgg atctccgcga gcaacgcgtc gcggagtcct ggccgcggcg cgaccggggt
     4261 gacgctggac ccgacggcgc cgacaacttc ctcatgacgg gaaagtgccg ctcggcaggt
     4321 ggggcagccg gccaggtggg cttcgaactc ctggcgctcc tcggggtcca gcaccccgag
     4381 ggcataggcg gcaaccgcat catccggatc ggggcgggag gatggtcggt gagctgtgcc
     4441 gtcgctcatt cgagatctcg cttgcgagat ggagggattc gggtcgccga gtcggcagac
     4501 ccttggccgg gctcccccat gcgatccgcc aagcgccgca tggccacccg aatctgggac
     4561 ttgacggtac cgagcgggcg ttccaggtgg tcggcgattt ggcgctggct caggccggtg
     4621 aagtacgcca gttccagcag gtggcgctga tcgggcacca gttcgtcgag tgcctgtcgc
     4681 acctcctggc ggtgctgctg gatcatcgtc tgctcctctg ggccagggtc cgggtcggga
     4741 atccaggaag cgagatcgac ttccatccca ccgatcgagt gctcgcgtcg ccgccgcagg
     4801 agatcgatcg cccggttccg gacagcccgc agcagccagc cagcaaagga gccccgggag
     4861 gagtcgtacg attccggctc ctgccaaagc cggagaaaga catcgtgcgt cgcttcctcg
     4921 gacaaggccg ggttgcggag gatcgacaac gccaggccgt tgacctgagc tgcatagcga
     4981 tcgtagagcg tggcgaaagc atcctcgtcg tgcccgcgaa cacgacgcat cagcacgaga
     5041 tcgctctcct ggcgctccaa ttcaaggggg agctctgcgg cgtagcgcct cgtcggcggg
     5101 gccacatggg gtggcacagc cggtttgacc gacctccgag ttcgctcagg ttccgtctgc
     5161 cctgcatcac tcggtcctgc cggagcgccg tcttcgtcgt ggcgccgcgt tttgggcgcg
     5221 tctcggatcg gccaacgacg gcggctgccg ttctcactcc tcactacgca gttccgggcc
     5281 gagcggatga gatcgacagg ttgtccagcg tgatctggag tgtggacacc ggacaagctc
     5341 ctgatccatg ggcaatctcg acatgcttct cggaattatc ctaccagccc gactacgggc
     5401 gtgcgccaat ggtcgaggcg tgatggtcag tggcacggcc ttcttgacga acgcgtgtaa
     5461 tgatgtgatg ataattacac accatgtagg gacgggcacg gttacaggag agaag
//
LOCUS       DASYWL010000295         7658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_222220, whole genome shotgun sequence.
ACCESSION   DASYWL010000295 DASYWL010000000
VERSION     DASYWL010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7658
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222220"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /gene="coaBC"
                     /locus_tag="VGR22_10400"
     CDS             complement(<1..818)
                     /gene="coaBC"
                     /locus_tag="VGR22_10400"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEV2129017.1"
                     /translation="MPLTDGFEVPPSLLHGKRVVFGVTGGIAAYKAADLVSWLVQQGV
                     QVRVTMTKAATEFVAPMTFEVITRQPVYIDVFDGWGGDEAGHVTLASMADAVLVVPAT
                     ANTIAEMAHGFTGDMLAAVLLSTTAPIVVAPAMEHHMWHHPATQANVRLLQERGVTIV
                     GPERGHLASGASGDGRLASRETVVAALRRVLARSGPLKGRTVVVSAGGTYEAIDPVRF
                     IGNRSSGQMGVAIVEAAHDAGATVRSVVGPTVVVPPVTGHVERVKSALEMAVAV"
     gene            complement(805..1905)
                     /gene="ltaE"
                     /locus_tag="VGR22_10405"
     CDS             complement(805..1905)
                     /gene="ltaE"
                     /locus_tag="VGR22_10405"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HEV2129018.1"
                     /translation="MTDDMIQHHPNATLIDLRSDTVTRPTPAMRQAMAVAIVGDDQYG
                     EDPTVDRLERLGAEKLGKEAAVFVPSGTMGNVSALMAWCGRGDEVILGDESHIYWFES
                     GSPAVLGGITQFVLPTELDGTLDVDRIAKAVRPERAGYPRTSVISIENTHNRRSGRVL
                     PMVYLRELRAVSQRLGVPVHMDGARIFNAAAALGVTAAEIAQHVDSVQFCLSKGLGAP
                     VGSMVAGSATFIDKVRRQRKLLGGAMRQLGLIAAAGIVALEQMVDRLPEDHARAKQLA
                     EGMAGVDGISADPAIVETNIIVIGVDPRIDQAKVVDRLKANGLLVSNYGAVGLRMVTH
                     HDVDDATIDRALDIVGKTVPPMMEGEATRAAD"
     gene            complement(1940..3376)
                     /locus_tag="VGR22_10410"
     CDS             complement(1940..3376)
                     /locus_tag="VGR22_10410"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV2129019.1"
                     /translation="MTYDNETRWRRGARRPWIPEVIADRYEVERELGAGAAAVTVAAR
                     DRRLGRQVAVKILRPGDEFDSAFSQRFTREARAAASINHPNVVDVYDVGQEGDLLYLV
                     MQFVDGTDLKRVIEREGALPWQRAVEIARGVLAGLTPIHAAGIVHRDVKPQNVLIGTN
                     GEIKVTDFGVAHVERDASLTAAGTTVGTAVYMAPEQAQGNAPSPAADVYAVGVMLYEM
                     VTGRLPFDEPTALATMLAHIQQEPDPPVAPRGREPIPEGIMQVIRQALAKDPRARFRS
                     AAAMQRALEHPGLAMKAPRSSGDTGSPTRVVPAAMPPRSRARSTGAWGIPAAPRYEAP
                     APESAGRGFGATFAMLLLVLAVVFAAVAGAMWAINERPDLFGGSDGGDGVPTSVPQPT
                     DTPEPTPTPTDEPEQPVVIDPIDEEPAPDPTPTPTPRPPTPTPQPPTPTPQPPTQVIV
                     EQEPTPASTDEEVPQIIEPIDNSTEPAN"
     gene            3489..4775
                     /locus_tag="VGR22_10415"
     CDS             3489..4775
                     /locus_tag="VGR22_10415"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2129020.1"
                     /translation="MASSRGIGFDRERWRILIWMCVLITANQFGFGAIVPVVPLFAQQ
                     FGVSQSAIGLTIAVYGLARFLVNVPASRVADAHGRRNALAVGGLITVIGTAASAMAPN
                     YELFLIARFMAGAGAAFVLTGGQIVVADISTSANRGRVMAIYQGVFLVSVGAGAFPGG
                     WLAEHISLPAPFWVAAGLAGIVSLLAWAFVPETRQYAHRSAARAGAAAPLSSREQLAA
                     VMAVPGFVLISIVGFATVAARTGALFNVIPVFAEERIGLGADQIGIGLGMVSIIGLLF
                     VYPSGLLVDRFGRKAIIVPSTLFSSLGMMLFGLATSFELYMVASVAWATASGISGATP
                     AAYAADIAPPGMAAPAMGLFRAISDSGYVIGPLALGALADATSSVTALRVTAATLLVI
                     GTLFALRAPESLKRDRGDVIEARSRRGVPNAPPPAR"
     gene            complement(4720..6564)
                     /locus_tag="VGR22_10420"
     CDS             complement(4720..6564)
                     /locus_tag="VGR22_10420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2129021.1"
                     /translation="MFPPLPGEQEIEHPWYERVMPASLRLRLVLPFVVLMALVLFVLT
                     LVFGNRARDIYTERLAESLVAQARTIADVAVLASDEAGTELDLAAIVQRLGTSGDRRV
                     SLIGPDGVVLADTAVEDPDNLENHNTREEVIEARADGTGLAERRSASVGVQYLYAATL
                     LDDGSDRVLRLAVPLDDVNAVVDRVQRYLLVAAAVALALTAAVALFIGVRLARPLEKL
                     RHHAQRVARGDLETEVEPSSTLEIDEVGYAFNMMTHALRTSLADLDRARTRLEAVLAG
                     LNDGVVLTDINGQVLRLNRAAERLLGINQDQAAGRPFIQIARDHELDAQLRAALRGEP
                     TRRVAVEHGLQRRILQMTATTVSGKAEQLGLVVLRDITELRRLEGVRRDFVANVSHEL
                     RTPLTSIRALVETLQGGAVDDQELKQEFLERIIREVDRLTALVEDLMDLARLEAGRTP
                     LNPEPAPATEVLHAAGERLREQVARAQLRLEYDLSESLPEVLVDRRRIEQVVLNLVHN
                     AIKFTPAGGNITIAGRTQGDMVVVEVRDTGVGIPKEEQERLFERFYKSDKARRTEGSG
                     LGLAIAKHIVETHGGDIAVESEVGEGAIFRFTLPVVERSARLGATALR"
     gene            complement(6566..7354)
                     /locus_tag="VGR22_10425"
     CDS             complement(6566..7354)
                     /locus_tag="VGR22_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2129022.1"
                     /translation="MASDASHAPKKARILVVDDEPTVREMVGLNLRADGYEVVFAEDG
                     NEALAVARSTRPNLIVLDVMLPGRDGFEVCRTLRAESSVPILLLSARGEEFDRVLGLE
                     IGADDYLAKPFAMRELVARVRAMLRRQQFSREYASEAAEPMEIPPDEPAKDDIVTVGD
                     IELSEARHEAWIRGQPLNLTSKEFALLRYFVRHPGIVLTRDALLREVWGYDYPIGTRT
                     VDVHVSWLRQKIEEDPHNPRRIRTVRGHGYRLVPIGATTAKDRD"
BASE COUNT         1213 a         2743 c         2359 g         1343 t
ORIGIN      
        1 tcgacggcca cggccatctc cagcgccgat ttgacccgct cgacgtggcc tgtaaccggc
       61 ggcacgacca cagtcggccc aaccaccgag cgcaccgtcg ccccggcgtc atgggcggcc
      121 tcgacaatcg cgacacccat ctgccccgag gagcggttgc cgatgaagcg caccgggtcg
      181 atcgcctcgt aggttccacc ggctgagacg acaactgtcc gccccttgag tggcccggaa
      241 cgcgccaaca cgcggcgcag cgcggcgaca actgtttcac ggctcgccag ccgtccatcc
      301 cccgacgctc ccgacgccaa atgcccgcgc tcggggccca caatcgtcac cccgcgctcc
      361 tgcagcagcc gcacgttggc ctgggtcgcc gggtggtgcc acatgtgatg ctccatagct
      421 ggcgcgacca cgatcggcgc tgtcgtggac agcagtaccg cagcgagcat gtctccagtg
      481 aacccgtgcg ccatttcggc gatggtgttc gcggtcgccg ggacgaccag caccgcgtcg
      541 gccatcgagg caagcgtcac atggcccgcc tcgtcaccgc cccacccatc gaagacatcg
      601 atgtagaccg gctgacgggt gatcacctca aaggtcatcg gcgcgacgaa ctccgtcgcc
      661 gccttcgtca tcgtcacccg cacctgcaca ccctgctgga caagccagct gaccaggtcg
      721 gccgccttgt aggcagcgat gcccccggtc acgccgaaca cgacccgctt gccatggagc
      781 agcgacggcg gcacctcgaa tccgtcagtc agcggcacgg gtcgcctctc cctccatcat
      841 cggcggaacc gttttgccca cgatgtcgag tgcccggtcg atggtggcgt catcgacgtc
      901 atggtgggtg accatccgca gcccgaccgc accgtagttc gataccagca gcccatttgc
      961 cttgagccgg tcgacgacct ttgcctggtc gatccgcggg tcgaccccga tcacgatgat
     1021 gttcgtctcg acgatcgccg gatcggccga gatcccgtcc acgcccgcca tcccctccgc
     1081 gagctgcttc gccctcgcgt gatcctccgg cagccggtcg accatctgct cgagcgcgac
     1141 gatgccggcc gccgcgatca ggcccaactg ccgcatcgcg cccccgagca acttgcgctg
     1201 ccgccgcacc ttgtcaatga acgtcgccga accggcgacc atcgatccga cgggcgcccc
     1261 aagccccttg ctcaggcaga actgcacact gtcgacgtgc tgcgcgatct ccgccgccgt
     1321 cacaccgagc gccgccgcag cgttgaagat ccgggcgccg tccatatgca ccggcacgcc
     1381 gagccgctgc gacaccgcac gcaactcgcg caggtagacc atcggcaaca ccctgccgga
     1441 gcgccggtta tgggtgttct cgatcgaaat gacgcttgtc cgcgggtagc ccgcccgctc
     1501 cgggcggacg gccttcgcga tacgatcgac gtcgagtgtg ccgtccagct ccgtcggcag
     1561 cacgaactgg gtgatgccgc cgagcaccgc cgggctgccg ctctcgaacc agtagatgtg
     1621 cgactcgtcg ccgagaatca cctcatcgcc gcgaccacac catgccatca gcgcggaaac
     1681 gttccccatc gtgccgctcg gcacgaagac agccgcctcc ttgcccagct tctcggcccc
     1741 gaggcgctcc agccgatcaa cggttggatc ctcgccgtat tggtcgtcgc caacgatggc
     1801 cactgccatg gcctgacgca tcgcgggggt cggacgggtg actgtatcgg agcgaagatc
     1861 gatgagggtg gcgttgggat ggtgctgaat catgtcatct gtcatctttc ctggtcctga
     1921 tgctggatgg tcgtcctcgt caattcgcgg gttcggtgct gttatcgatc ggctcgatga
     1981 tctgcggaac ctcctcgtct gtcgaggctg gcgtcggctc ctgctcaacg atcacttggg
     2041 tgggtggttg cggcgtaggc gtcggcggtt gcggtgtcgg tgtgggcggc cgtggtgtgg
     2101 gagtcggtgt cggatcaggc gctggctcct cgtcgatcgg gtcaatcaca accggctgct
     2161 ccggctcgtc ggtcggcgtc ggcgttggct ccggcgtgtc ggtcggctgc gggacgctcg
     2221 tcggcacgcc atcgccaccg tcgctcccgc cgaacagatc cgggcgctcg ttgattgccc
     2281 acatcgcccc ggcgaccgcc gcaaagacga ccgccagcac caggagcagc atcgcgaagg
     2341 tcgccccaaa ccctcgtccg gcactctcgg gagccggcgc ctcgtatcgt ggtgctgcag
     2401 ggatgcccca cgcccccgtc gacctggccc tcgaccgtgg tggcatggca gcggggacca
     2461 cccgggttgg gctgccagta tcgcctgaag atctgggcgc cttcatggcg aggcccgggt
     2521 gctcgagggc gcgctgcatc gcagcggcgc tccggaaccg tgctcgcgga tccttggcga
     2581 gcgcctgccg aatcacctgc atgatccctt ccgggatcgg ctcccgcccc cgcggtgcga
     2641 ccggcgggtc cggctcctgc tggatgtgcg ccagcattgt cgcgagcgcg gtcggttcgt
     2701 cgaacggcag gcgcccggtc accatctcgt acagcatcac cccgaccgcg taaacgtctg
     2761 cggccggtga tggcgcgttc ccttgcgcct gctccggcgc catgtagaca gccgtaccga
     2821 cagtggtgcc cgccgccgtg agcgacgcgt cccgctccac gtgcgcgacg ccgaaatcgg
     2881 tgaccttgat ctcaccgttg gtgccgatca gcacgttctg cggcttgacg tcgcgatgga
     2941 cgatgcccgc cgcatggatc ggagtgagcc ccgcgagcac ccccctcgcg atttcgaccg
     3001 cccgctgcca cggtaacgcg ccctcacgct cgattacccg cttcagatcg gtgccgtcga
     3061 cgaactgcat caccagatag agcagatcgc cctcctggcc gacatcgtag acatcaacca
     3121 cgttcggatg gttgatcgaa gccgccgctc tcgcctcacg cgtaaaccgc tgcgaaaacg
     3181 ccgaatcgaa ctcgtccccg ggccgcagga tcttgaccgc cacctgccgc ccgagccgcc
     3241 ggtcacgagc ggccaccgtc accgctgccg cgccggctcc cagttcccgc tctacctcgt
     3301 agcgatcggc gatcacctcc ggaatccacg gccgccgcgc tccccgccgc caccgagtct
     3361 cattgtcgta cgtcatgcaa tgtccctcgc cggtgccaac gtcggcccat tcccgtaccg
     3421 gtcgatcccg gcatcttgcg ccaaacgttg cttccaacga tgctgtgcat acgatgggag
     3481 gggcaacggt ggcgtccagc cggggtatcg ggttcgatcg ggagcggtgg cgcatcctca
     3541 tctggatgtg cgtgctgatc accgccaacc aattcggctt cggggcaatc gtaccagtcg
     3601 tgcccctctt cgctcagcag tttggcgtct cccagtcggc aatcggtctc accatcgccg
     3661 tgtacggtct cgcgcgcttt ctcgtcaacg tcccggccag ccgcgtggcc gatgcccacg
     3721 gccgccgcaa cgcgcttgcc gtcggcggcc tcatcaccgt catcggcacc gcggcgagcg
     3781 ccatggcgcc gaactacgaa ctcttcctga tcgcacgctt catggcgggc gcaggggctg
     3841 ccttcgtgct caccggcggg cagatcgtcg tggcggacat ctccaccagc gccaaccgtg
     3901 gccgtgtaat ggccatctac cagggggtct tcctcgtcag cgtcggtgcc ggtgccttcc
     3961 ccggcggctg gctggccgag cacatcagcc tgcccgcacc gttttgggtg gccgctggtc
     4021 ttgccgggat cgtctcgctg ttagcttggg ccttcgtgcc ggagacccgc cagtatgccc
     4081 accggagcgc cgctcgcgcc ggggctgccg caccgctctc ctcgcgagag cagctcgccg
     4141 ccgtgatggc cgtccctggc ttcgttctga ttagcatcgt cggcttcgcg accgtcgccg
     4201 cacgcaccgg cgcgctcttc aacgtgattc ctgtcttcgc cgaggagcgg atcggcctcg
     4261 gcgctgacca gatcggtatc gggctcggca tggtgagtat catcggcctg ctcttcgtct
     4321 acccctccgg gttgctggtc gaccgcttcg ggcgcaaggc gatcatcgtg ccgagcacgc
     4381 tcttctcctc tctggggatg atgctctttg gcctcgcgac cagcttcgag ctctacatgg
     4441 tggcatccgt ggcgtgggcg accgcctccg gaatcagcgg agcgacgcca gccgcctacg
     4501 ctgccgacat cgcacctcct ggcatggccg ctccggcgat ggggctcttt cgcgcaatct
     4561 ccgactctgg ttacgtgatc ggcccactgg cgctcggcgc gctcgccgac gcgacctcgt
     4621 ccgtgaccgc cctccgggtt acggcagcga cactgctcgt catcggcaca ctcttcgcgc
     4681 tgcgcgcgcc ggagagcctc aagcgggacc ggggcgacgt catcgaagcg cggtcgcgcc
     4741 gaggcgtgcc gaacgctcca ccaccggcaa ggtgaagcgg aagatggctc cctcgccgac
     4801 ttcgctctcg accgcgatat cgccaccatg ggtctccact atgtgcttgg cgatcgcgag
     4861 cccgagcccg ctgccctccg tccggcgtgc cttgtcagac ttgtagaacc gctcgaagag
     4921 ccgctcctgc tcctccttcg gaatgcccac ccccgtatcg cgcacctcga cgacgaccat
     4981 gtcgccctgc gtcctgccgg cgatcgtgat gttgccgccc gccggcgtga acttgatggc
     5041 gttgtggacc agattgagga ctacctgctc gatgcggcgc cgatcaacca gcacctctgg
     5101 gagcgactcc gacaggtcgt actccagtct gagttgggcg cgagccacct gctcgcgcag
     5161 gcgctctcct gcggcgtgaa gcacttccgt ggccggagcg ggctcggggt tcagcggcgt
     5221 acgccccgcc tccagccgag ccagatccat gaggtcttcg acgagcgccg tcagccggtc
     5281 gacctcgcgg atgatccgct ccaggaattc ctgcttcagc tcctgatcat ctacggcgcc
     5341 gccctgcaac gtctccacaa gcgcccggat ggaggtcagt ggcgtgcgaa gctcgtggga
     5401 gacattggcg acaaagtccc gtcgcacccc ttccaaccgg cgcagctcgg tgatgtcacg
     5461 aagaaccacg agccccagct gctcggcctt gccgctcaca gtcgtggctg tcatctgcag
     5521 aattcgacgc tgaaggccat gctcgacggc cacccgcctg gtcggctcac cccgcaatgc
     5581 ggcccgcagc tgggcgtcca gctcgtgatc acgcgcgatt tggataaagg ggcgcccggc
     5641 agcctgatcc tgattgatgc cgagcagacg ctccgccgcc cggttgagtc tcagcacctg
     5701 gccgttgata tccgtgagca ccaccccatc attcaggcca gcgagcacag cttcgagccg
     5761 cgtgcgggcc cggtcgagat cggccagcga tgttcggagg gcgtgcgtca tcatgttgaa
     5821 ggcgtagccc acctcatcga tctcgagcgt cgaagacggc tcaacctcgg tctcgagatc
     5881 accccgagcg acccgctgcg catggtgccg cagcttctcc agcggccttg ccagccggac
     5941 accgatgaag agcgcgaccg cagccgtcaa tgccaacgcc acggcagccg ctacaagcaa
     6001 gtaacgctgc acacggtcca ccacggcatt gacgtcgtcg agcggcacgg cgagacgcaa
     6061 cacgcggtcg gatccgtcgt cgaggagtgt cgcggcgtag aggtactgca cgccaacaga
     6121 ggcactccgt cgctcggcca gtccggtacc atctgcccgc gcctcgatga cttcctcacg
     6181 ggtgttatgg ttttccagat tgtccggatc ttccactgcc gtatccgcga gcaccacgcc
     6241 gtcgggcccg atgaggctga cccggcgatc gcccgacgtt ccgagccgct ggacgatcgc
     6301 cgcgagatcc agttccgtgc cagcctcgtc cgaggcgagc acggcgacat cagcgatggt
     6361 tctcgcttgg gcaacaagcg attcggcgag ccgctcggtg tagatgtctc gagcgcgatt
     6421 gccgaacacg agcgtcagca cgaagagcac gagcgccatc agcacgacga agggcaggac
     6481 caaacggagt cgcagcgaag ctggcatgac ccgctcgtac cacggatgtt cgatctcctg
     6541 ctcaccgggt agtggcggga acacgtcagt cgcgatcctt cgctgtggtg gcaccgatcg
     6601 ggacgaggcg atacccgtgc ccgcggaccg tccgaatccg ccgtgggttg tgcggatcct
     6661 cctcgatctt ttgccggagc cagctgacat gcacgtcgac agtacgcgtg ccgatcggat
     6721 agtcgtagcc ccacacctcg cgcagcagcg cgtcacgagt aagcacgatg cccgggtggc
     6781 gcacgaagta gcgcagcagg gcgaactcct ttgaggtgag gttgagtggc tggcctcgga
     6841 tccacgcctc gtggcgggcc tcgctcagct cgatatcgcc aaccgtgacg atgtcatctt
     6901 tggctggctc atcgggcgga atctccatcg gttcggcagc ttcactcgca tactcccggg
     6961 agaactgttg ccggcgcagc atcgcgcgaa ctcgcgccac caactcgcgc atggcgaacg
     7021 gcttggcgag gtagtcatcc gcaccgatct cgaggccgag cacgcgatcg aactcctcgc
     7081 cgcgggctga aagcaacagg atcggcacgc tcgactcggc ccgcaaggtt cggcagacct
     7141 caaaaccatc ccgcccgggc agcatcacat ccagcacgat cagattcggt cgggtactcc
     7201 gggccactgc gagcgcctca ttgccatctt cggcgaaaac gacttcgtac ccgtctgcgc
     7261 gcaggttcaa gccgaccatc tctcggacgg tcggctcgtc atctaccacg aggatacggg
     7321 ctttcttcgg cgcgtggctg gcgtccgacg ccatgcttgc tcctggtgct cgcgcagcga
     7381 cccgccatcg agcgtcggtg gcgtggtttc acactcagca taccgggcgc catcggctct
     7441 cagccagcct gctgcttctg cgagcgtttg ctcggccgcc agttccatcg accgagcagc
     7501 accacgtgcc cgggacaatg acggcagcga gcagcgccca gaccgcagta acgagccatt
     7561 tgccttggac gaagacgcga agtcggtgat gcggctcagc acctggcagc tactcctgat
     7621 gaggccaccg ggctcgaatc gtcatggatt ggcatgtg
//
LOCUS       DASYWL010000296         2436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_259800, whole genome shotgun sequence.
ACCESSION   DASYWL010000296 DASYWL010000000
VERSION     DASYWL010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259800"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VGR22_10430"
     CDS             complement(<1..522)
                     /locus_tag="VGR22_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129023.1"
                     /translation="MAIELGLDFYVFRGDLAGAGGWLQRAHRLLDGIAPSPERGWLEL
                     WEAHIALLEHNDVAFAKRACSEILALAESLDVVDLEILALALSGLALVNEGRVDDGMR
                     RLDEATTAALAGEMRDVDAVVTACCYHIYACEHVRDVPRAIAWCERAEDVSRRWSYRS
                     MFAFCRCHYAAVLI"
     gene            985..1896
                     /locus_tag="VGR22_10435"
     CDS             985..1896
                     /locus_tag="VGR22_10435"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV2129024.1"
                     /translation="MRPLQIGVQLPEVEYEYDWPQLKEMALLAERIGLDSIWLGDHLM
                     YPPSGSKPARGPFEAWSTLSALAAATERVQLGPLVASLGFHTPPMIAKKAATIDRISN
                     GRFILGVGSGWNEAEYRGYGFPYDRRVSRFEEAFAIIRELFATGESTFHGGFYDIEGA
                     LLFPRPVQPGGPPLMIGSFGDRMLRITLPHVQMWNAWNHDYGNTRDGLAALLARIDAA
                     CEAAGRDPAALERTVCPLVQMTGGIGRPSGSGEVVPPIDGTDPAALAAELTALAEMGI
                     SHVMLVLDPITPNAIAELEPVLRLLDT"
     gene            1935..>2436
                     /locus_tag="VGR22_10440"
     CDS             1935..>2436
                     /locus_tag="VGR22_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129025.1"
                     /translation="MTERDPIRRAALLATPPLLLMTTAAAFRGFQRQFGDRLGYLAGF
                     LVYWIGWCMLLPLWVLGPRGLRELFRPARHSRKDVALLSAPPALGMVAAFPSAAREAT
                     PAVIAGSVAIAVVNGTLEEVLWRGTYQAVFPDAPVTGLYPAVGFAVWHYAPQTIHPTH
                     PHSNPTS"
BASE COUNT          436 a          829 c          768 g          403 t
ORIGIN      
        1 gatcaacaca gccgcgtaat ggcagcgaca gaaggcaaac atcgagcggt acgaccagcg
       61 cctggaaacg tcttccgccc gttcacacca tgcaatggcg cgagggacgt cccgaacgtg
      121 ctcacatgca tagatgtgat agcaacaggc ggtgacgaca gcgtcgacgt cgcgcatctc
      181 acccgccaac gcggccgttg tcgcctcatc cagtcggcgc atgccgtcgt ccacccgccc
      241 ttcattcacg agtgccaggc cactcagggc gagggcgaga atctccagat cgacaacgtc
      301 gagggattcc gcaagcgcca gaatctctga acaggcacgc ttcgcgaacg caacatcgtt
      361 gtgttccagg agcgcgatat gcgcctccca gagctcgagc cagccgcgct ctggcgaggg
      421 ggcgatgccg tcgagcaatc gatgtgcccg ctgcaaccag ccgccggcac cggccagatc
      481 gccacgaaag acgtagaaat ctagccccaa ctcgatggcc atgcgggccg cacccaggcg
      541 atcgccagcc tgtcgataga gccgatatgc tcgttctacg tgcagggaag agagttggga
      601 cgtcgtcgag ccaccaggct gctgtcgcga gtccctccag ggcctcgggc gattcctcgg
      661 tgtcaaccgc acgctggaag gcttccagcc ccttctgcca ctccccctgt gcgagtgcct
      721 ggttcccggc acgaagcagc gcacccgaag gttcaggcac gctcctggcg gttactggaa
      781 caccgagttg cctgcgtcgt cgatcgctgg acatggtggt ccatctccgg aagaccctca
      841 tgatacctga acctctggtg ccgcatctga gataccggcg gcgcaaggat gtaccatccc
      901 gcaagtgctg tgatgccgct atactgcgcc attcgaatcc cgcatgccgg gacagggcat
      961 cgtcaccaag cgaggaacct accagtgcga ccactccaga tcggcgtgca gctccccgaa
     1021 gtcgaatacg agtacgactg gccgcaactg aaggagatgg cgctgctggc cgaacggatt
     1081 gggctcgact cgatctggct cggcgaccac ctgatgtacc ccccgagtgg gtcgaagccg
     1141 gcacgcggcc cgttcgaggc gtggagcacc ctctcggcgc tggctgcggc gaccgaacgt
     1201 gtgcagctcg gcccgctggt cgcgagtctc ggcttccaca ccccaccgat gatcgccaag
     1261 aaggccgcga ccatcgaccg aatcagcaat ggccgcttca tcctcggcgt cggctcaggc
     1321 tggaacgagg cagagtaccg aggctatggg tttccgtatg accggagggt gagccgattc
     1381 gaggaggcgt tcgcgatcat ccgcgagctc ttcgcgaccg gcgagagtac cttccacggt
     1441 ggcttctacg acatcgaggg agcgctgctc ttcccgaggc cagtgcagcc cggcggcccg
     1501 ccgctgatga tcgggtcctt cggcgatcgg atgcttcgaa tcacgctgcc acatgtgcag
     1561 atgtggaacg cctggaacca cgactacggc aacacccgcg atgggcttgc cgcgctgctg
     1621 gcgaggatcg acgccgcctg cgaggcggcc gggcgcgacc cggcagcgct ggagcgcacc
     1681 gtctgtccgc tcgtgcagat gaccggtggc attggccggc cgtcgggatc gggcgaggtc
     1741 gtgccaccga tcgacggaac ggacccagcg gcgctcgccg cggagctgac cgcgctggcg
     1801 gagatgggta tcagccatgt gatgctggtc ctcgacccga tcacgccgaa cgccatcgcc
     1861 gagctcgaac cggtgttgcg cttgctcgac acctagcacg attcaggctg tccaccgcag
     1921 caaggagcgc cgacatgacc gagcgagatc ccattcggcg agccgcgctg ctggcaaccc
     1981 cacctctgct tctgatgacc accgccgcag catttcgcgg gttccagcgg cagttcgggg
     2041 atcgactcgg ctacctcgcc ggattcctcg tctactggat cggctggtgc atgctgctac
     2101 cgctgtgggt gcttgggcct cgagggctgc gcgagctctt ccgtccggct cggcactctc
     2161 gaaaggacgt cgcgctgctc tccgccccac cggcgctcgg tatggtggcc gcgttcccga
     2221 gcgcagcacg cgaggcgacc ccggcggtga ttgccgggtc ggtcgccatc gccgtcgtga
     2281 atggcacact ggaggaggtg ctgtggcgcg ggacctacca agcggtcttc cccgatgcgc
     2341 cggtgaccgg cctctaccca gcggtcgggt tcgctgtttg gcactacgct ccgcagacga
     2401 tccacccgac ccatccccac agcaacccca caagcc
//
LOCUS       DASYWL010000297         7318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_540899, whole genome shotgun sequence.
ACCESSION   DASYWL010000297 DASYWL010000000
VERSION     DASYWL010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7318
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540899"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VGR22_10445"
     CDS             complement(<1..676)
                     /locus_tag="VGR22_10445"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEV2129026.1"
                     /translation="MAGDYTPQKRDKFTFGLWTVGNPGRDPFGDPVRGPLDPHRSVAK
                     LAELGAWGVTLHDNDLVPFDATPKQRDSIVVDFKRVVDENGLVVPMMTTNLFTHPIFR
                     DGAFTSSDASVRAFALQKTMRSIDISAELGAETYVFWGGREGTETDAYRDPRDAMRWF
                     REAINYLTEYVRDQGYGLRFALEPKPNEPRGDIFLPTIGHALAFIETLDQPDMVGVNP
                     EVAHDVM"
     gene            complement(757..1929)
                     /locus_tag="VGR22_10450"
     CDS             complement(757..1929)
                     /locus_tag="VGR22_10450"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2129027.1"
                     /translation="MAEIGVGLVGYKFMGRAHSNAYRQVPHFFDVDPKPRMVAIAGRN
                     EEACREAAEQLGWEGYETDYHRLVARDDIQLVDVSTPGYNHHEIVLAALAAGKHVLCE
                     KPLANTLEEAQEMLTAARKAGTVNMVNFNYRRVPAVQLAKRLVDEGKLGEIRHFRARY
                     LQDWILDPQFPLVWRMQKELAGSGALGDIGAHITDLAMYLAGPVTEVVGSMETYIRER
                     PIEVESGGGSGLTAQSGQEVGEVTVDDATSYLARFESGAMGTFDASRLAPGRRNQNTF
                     EINGSKGSVTFDLERLNELQVYFVDDEEGLQGFRTIDVTEPAHPYMENWWPAGHIIGY
                     EHTFVHVVRDLMEGIGKGENPQPDFEVGFRNQAVLDAVIRSTETSTWEKPAQPDLG"
     gene            complement(1942..2943)
                     /locus_tag="VGR22_10455"
     CDS             complement(1942..2943)
                     /locus_tag="VGR22_10455"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEV2129028.1"
                     /translation="MTRPVTLFTGQWADLPLEELAPKAKEMGFDGLELCCWGDHFEVQ
                     RAVSEPDYVDSRWDILKKNNLKCFAIGNHLVGQAVCDAVIDQRHKGIVPPHVWGDGDP
                     EGVRQRAAKEMQDTALAAAKFGVPVVTGFTGSAIWHVLAMFPPVPPQLIEDGYRDFAD
                     RWNPILDVFDEVGVKFALEVHPSEIAYDFWTTKRAMDAIDHREAFGINYDPSHLYWQM
                     LDPVAFIYEFGDRIFHAHVKESILVLDGRNGILGSHLLFGDHRRGWDFVSPGRGGVPF
                     ERVFRALNDVGYDGPLSVEWEDSGMNREQGARESVELVRRLDLVPSDVAFDAAFQRS"
     gene            3197..4750
                     /locus_tag="VGR22_10460"
     CDS             3197..4750
                     /locus_tag="VGR22_10460"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEV2129029.1"
                     /translation="MGQSAEHGGSRVIIGNRYSVDLDHPVGIGGMAMVYQGFDLQSRR
                     TIALKTLRTEYQRDPNSRARFRREARAMAFVNHPNLVTIYDLYEEQDGSWVVMEYVDG
                     ETLKELVQREGPLPPESVALILQQVANALGHIHERNMVHLDIKPQNIMITASGEVKII
                     DFGLAQPPGARIEAGGGIAFGTVAYLAPEQARGDAVGVETDVYSVGCVVYELLTGQLP
                     FDIPEGPEQKRQLMYAHLEELPVAPSNVCPELELPAWVDDVVGWTLVKNPSDRIHDVR
                     TVARLFRAGLEGEEIDDAGRTTVLEHRAGRQRIEPSHRLRLRWRERVAELDGADEDAI
                     PELREDRSGPLGRLYRRGGRLARRSRRTRRFLWRLFLVMLTGTLLLGLILFLRRGPEA
                     LVENFLSVAPGTETTVVVDRLNIRGGPTIDARPLGVVPEGAEVRVIGLSETRGEYRFW
                     PIETEIDGEPVSGWAWDGGLQPNEWTGRLSWVQGIVERVQSAQDSASDAWDRVTGWPL
                     GGVIVPSEL"
     gene            5005..6264
                     /gene="nadA"
                     /locus_tag="VGR22_10465"
     CDS             5005..6264
                     /gene="nadA"
                     /locus_tag="VGR22_10465"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006883.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEV2129030.1"
                     /translation="MANNMNPGTATLWPETKPAEERKFKTLWLQDTQLEPAACVPEEG
                     IGAYDRLAAYDVHPNHQEKIRREYFRMPVEERDARIFALKEQLGDRLTILGHHYQRDE
                     VVQFADFQGDSYKLSQQAASQSKADYTLFCGVHFMAESADILGSPHQQVILPNLEAGC
                     SMADMANGDDVAAAWATLERLGITGAVPVTYMNSSAEIKAFCGRNGGIVCTSSNAERV
                     YDWAFERGDKLLFFPDEHLGRNTALKKGIPEEEMVVWDPFQPLGGNTEEELKAARIFL
                     WKGYCSVHARFSVEQIEKARAEHPDATVIVHPECRAEVVQAADMDGSTEYIIDIIRNA
                     PSGTTWAVGTEINLVRRLQSQMPDKTIFCLDPVICPCSTMYRIHSAYILWALEHLVEG
                     RVVNEISVEPDTAEAARVALDRMLAVP"
     gene            6300..6755
                     /locus_tag="VGR22_10470"
     CDS             6300..6755
                     /locus_tag="VGR22_10470"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:6435..6437,aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEV2129031.1"
                     /translation="MLIQVGEQAPDFELEDSSGQAFRLSDMQGLANVLLIFYPKDMTS
                     GURDQLVAVRDNFTSLQRNEIQPVGVNHGDAESHRSFREKYNFPFELLVDERLKVAAA
                     YGALNEQGNGISRSVVVVGKDGNVVFSESGAPAMPRVINAVKATAVADD"
     gene            complement(6849..>7318)
                     /locus_tag="VGR22_10475"
     CDS             complement(6849..>7318)
                     /locus_tag="VGR22_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEV2129032.1"
                     /translation="GPMAGGPVVITDAVAPLRNLDEPDQHARFVRSIRGRTVVSRMGR
                     PVGKVADVLYETKLSEDHVTGIESRRIIPRYIVVQTGSALKFGKRLTAVPAELVDADQ
                     DPVLLRITRAELCDAPRYDTSQPLSRQDEMALRRYFHVRNYWMTPRYDDEWVH"
BASE COUNT         1290 a         2297 c         2412 g         1319 t
ORIGIN      
        1 ccatcacgtc gtgcgcgacc tcggggttca cgccgaccat gtccggctgg tcgagcgtct
       61 cgatgaacgc cagcgcgtgg ccgatggtcg gcaggaagat gtcgccgcgc ggctcgttcg
      121 gcttcggctc gagcgcgaag cggagcccgt agccctgatc gcgcacgtat tccgtgaggt
      181 agttgatcgc ctcgcggaac cagcgcatcg cgtcgcgcgg atcgcggtag gcatcggttt
      241 cggtgccctc gcggccgccc cagaagacgt aggtctcggc gccgagctct gcgctgatgt
      301 cgatcgaacg cattgtcttc tgaagggcga aggcacgcac cgaggcgtcg gaggaggtga
      361 aggcaccgtc gcggaagatc gggtgcgtga agaggttggt ggtcatcatc ggcacgacca
      421 agccattctc gtcgaccacc cgcttgaagt cgacgacgat gctgtcccgc tgcttcggcg
      481 tagcgtcgaa ggggacgagg tcgttgtcgt gaagggtgac gccccaggcg ccgagctcgg
      541 cgagcttcgc gacgctgcgg tgggggtcca gcggcccgcg gacaggatca ccgaatggat
      601 cgcgcccggg gttgccgacc gtccacaagc cgaaggtgaa cttgtcgcgc ttctgtgggg
      661 tgtagtcgcc tgccatgcat cgatcctcct gctaacgctg cgtgttcctg ccacatcgcg
      721 ccgctgcaat tcgagaagag ttttcgggct atggggtcaa cccaaatcgg gctgggcagg
      781 tttctcccac gtgctggtct ccgtgctgcg gatgacggcg tcgaggaccg cctggttgcg
      841 gaatccaacc tcgaagtccg gctgcgggtt ctcgcctttg ccgatgccct ccatcaggtc
      901 acggacgacg tgcacgaacg tgtgctcgta gccgatgatg tggcctgccg gccaccagtt
      961 ctccatgtac gggtgtgccg gctccgtgac atcgatcgtc cggaagccct ggaggccctc
     1021 ctcgtcatcg acaaagtaga cctgcagctc gttgagccgc tcgagatcga aggtgaccga
     1081 tcccttcgag ccgtttattt caaaggtgtt ctggttgcgg cgccccggag cgagccggga
     1141 ggcgtcgaat gtccccatcg caccgctctc gaagcgagcc aggtagctgg tcgcgtcatc
     1201 gacggtgacc tcacccacct cctgaccgct ctgggcagtc agtccggaac ccccgccgct
     1261 ctcgacctcg atcggccgct ccctgatgta ggtctccatc gagcccacca cctcagtgac
     1321 cggaccggca aggtacattg cgaggtcggt gatgtgcgcg ccgatatcac cgagggcgcc
     1381 ggagccggcc agctccttct gcatgcgcca cacgagcggg aactgtggat cgaggatcca
     1441 gtcctgcagg tagcgcgcgc ggaagtgtcg gatctcgccg agcttgccct cgtcgacgag
     1501 gcgcttggcc agttggaccg ccggcacacg ccggtagttg aagttgacca tgttgaccgt
     1561 gccggccttg cgggcggcgg tcagcatctc ctgtgcctct tccagcgtgt tggccagcgg
     1621 cttctcacac aagacatgct tgccagccgc cagcgcggcg agcacgatct cgtggtggtt
     1681 gtagccgggg gtggagacgt ccacgagctg gatgtcatcc cgcgccacga gccggtggta
     1741 gtccgtctcg tagccttccc agccgagctg ctccgcagct tcgcgacacg cctcctcgtt
     1801 ccgcccggcg atcgccacca tgcgtggctt tggatccacg tcgaagaagt gcggtacctg
     1861 gcggtaggcg tttgagtgtg cacgacccat gaacttgtag ccgacgagac cgacaccgat
     1921 ctctgccatg tctctttccc cctagctccg ctgaaacgcg gcgtcgaacg cgacatcact
     1981 cgggacgagg tcgaggcgcc taaccagttc gaccgactcg cgtgctccct gctcccggtt
     2041 cattcctgag tcctcccatt cgactgagag cgggccatcg tagccgacgt cgttgagcgc
     2101 ccggaagacg cgctcgaatg gcacgccgcc gcgtcccggc gagacgaagt cccatccgcg
     2161 gcggtgatcg ccgaacaaga gatgtgaccc gaggatgccg ttccggccgt cgagcacaag
     2221 gatcgattcc ttcacgtggg cgtggaagat gcggtcaccg aactcataga tgaacgcgac
     2281 tggatcgagc atctgccagt agaggtgact cgggtcgtag ttgatgccga acgcctcgcg
     2341 gtggtcgatg gcgtccatgg cgcgcttggt cgtccagaag tcataggcga tctcggatgg
     2401 gtggacctcg agcgcgaact tcacccccac ctcgtcgaag acgtccagga tcgggttcca
     2461 gcggtcggcg aagtcccggt agccgtcctc gatcagctga ggtggtaccg ggggaaacat
     2521 cgccaacaca tgccagatgg cggacccggt aaacccggtg acaaccggca cgccgaactt
     2581 cgctgcggcc agggccgtgt cctgcatctc cttggcggcg cgctgccgga cgccctcggg
     2641 atcaccgtcg ccccagacgt gcggcggcac gatgcccttg tggcgttgat cgatcacggc
     2701 atcgcagacg gcctgcccca cgaggtggtt gccgatggcg aagcacttca ggttgttctt
     2761 cttgaggatg tcccaccggg agtcgacgta gtcgggctcg ctgacggccc gctggacttc
     2821 gaagtggtcg ccccaacagc agagctcgag gccgtcgaag cccatctcct tcgccttggg
     2881 cgcgagctcc tccagcggca gatcggccca ctgcccggtg aacaaggtga ctggtctcgt
     2941 catggattcc tcctttgcgc tccgggaact ttgggtgatc gctctgcttg tcgtttccag
     3001 gcactctccc gcggtgcgct tgtttggtta ttggagtgtg caatccccac ccgtgggtgt
     3061 caacaacccc atgtcgagcc agagtgagag cggtctggtg gtagagttcc ccaaggagac
     3121 ggcgacggga ccgggcctgc ggacgaaagc agcaggacga gcgagcctgg acggccgacg
     3181 gggtcagcag cgcgcgatgg gtcaatccgc agaacacggt ggcagccggg tcatcatcgg
     3241 caaccgatat agcgtggatc tcgatcaccc ggtcggcatc ggcggcatgg cgatggtgta
     3301 ccagggtttt gacctgcagt cccgtcgcac gatcgccctc aagaccctcc gcaccgagta
     3361 ccagcgtgat cccaattcgc gcgcccggtt ccggcgcgag gcgcgcgcga tggccttcgt
     3421 caatcacccc aacctcgtga ccatctacga tctctacgag gagcaggacg gctcctgggt
     3481 cgtgatggag tacgtcgatg gcgagacgct gaaggaactg gtgcagcggg aggggccgtt
     3541 gccgccggag tcggtcgcgc tcatcctgca gcaggtcgcc aatgcgcttg ggcacatcca
     3601 cgagcgcaac atggtccacc tcgacatcaa gccgcagaac atcatgatca ccgcatctgg
     3661 cgaggtgaag atcattgact tcggtctcgc gcagccgcca ggggcccgca tcgaggcagg
     3721 tggcggcatc gccttcggca cggtcgcgta cctggcgccg gagcaggcga ggggcgacgc
     3781 ggttggcgtc gagacggacg tctactcggt gggctgcgtc gtctacgaac tgctgaccgg
     3841 ccaactgccg ttcgacatcc cggagggtcc tgagcagaag cggcagctga tgtatgccca
     3901 tctcgaggag ttgccggtcg ccccgtcgaa cgtctgcccc gagctggagt tgcccgcatg
     3961 ggtcgacgat gtggtgggct ggacactggt caagaatccc agcgatcgaa tccacgatgt
     4021 gcgaacagtc gcgcgcctct tccgcgccgg acttgaggga gaggagatcg atgatgcagg
     4081 acgcaccacc gtcctcgagc accgtgctgg tcgacagcgc atcgagccct cacaccgact
     4141 ccggctccgt tggcgtgagc gcgtagcgga actggacgga gccgacgagg acgcgattcc
     4201 cgaattgagg gaggataggt ccgggcccct agggcgtctc taccggcgtg gggggcgact
     4261 ggcgcgtcgt tcccgacgaa cgcgtcggtt cctgtggcgt ctctttctcg tgatgctgac
     4321 cggcacgctg ttgctcgggc tgatcctctt cctgcggcgc ggtccggagg cacttgtcga
     4381 gaatttcctg agcgtcgcgc cgggcaccga gacgacagtt gtggtcgacc ggctcaacat
     4441 ccgcggcgga ccgacaatcg acgcacgacc gctcggcgtg gtgccggagg gcgcggaggt
     4501 gcgggtcatc gggctctccg agacgagggg cgagtaccgg ttctggccca tcgaaaccga
     4561 gatcgacggt gagccggtgt ccggatgggc atgggatggc gggctgcagc cgaacgagtg
     4621 gactggcagg ttgtcgtggg tccaggggat cgttgagcgg gtgcagagcg cgcaggatag
     4681 cgccagcgac gcctgggatc gtgtgacggg ctggccgcta ggcggggtga ttgtgccgtc
     4741 cgagttgtag gcgaggtgaa cccgaatcgg gacgaaatcc gcccgtcacg tccaactcag
     4801 tttccccctt gcttggcagg gctcccgaga ccagcacccc acaggacagc ttcatggcgt
     4861 accggctgta actaactgac aacatggact tagtgtcatg ttgacaatta ctcccaaacc
     4921 ccctaggctt tgcacagtac ttgtgagcga agagcgttcg cgaacggccg gagccagttc
     4981 attccggcgg gagagctcct ctacatggcg aacaacatga atcccggaac cgccaccctc
     5041 tggcctgaga cgaagccggc cgaggagcgg aagttcaaga ccctgtggct gcaggacacg
     5101 cagctggagc cggcggcctg cgtgcccgag gagggtatcg gcgcctacga ccggctggca
     5161 gcgtacgatg tgcatccgaa tcaccaggag aagatccgga gggagtattt caggatgccg
     5221 gtggaggagc gggacgcgcg aatctttgcg ctcaaggaac agctcggtga ccgcctgacg
     5281 atcctcggcc accactacca gcgtgacgag gttgtccagt tcgccgactt ccagggcgac
     5341 tcctacaagc tttcgcagca ggcagcgagc caatcgaagg cagactacac gctcttctgt
     5401 ggcgtccact tcatggccga gagcgccgac atcctcggct cgccgcatca gcaggtgatc
     5461 ctgccaaatc tcgaggcagg ctgctcaatg gccgacatgg ccaacggcga cgacgttgct
     5521 gcggcgtggg cgacgctgga gcgtctcggc atcactggcg ccgtgccggt cacctacatg
     5581 aactccagtg cggagatcaa ggcgttctgc gggcgcaacg gcggcattgt gtgcacctcc
     5641 tcgaatgcgg aacgggtgta cgactgggcg ttcgagcgcg gcgacaagct gttgttcttc
     5701 ccggacgagc atctgggccg gaacacggcg ctcaagaagg gcattcccga ggaggagatg
     5761 gtcgtctggg acccgttcca gccgctcggc ggcaacaccg aggaggagct caaggcggcg
     5821 cgcattttcc tctggaaggg ctactgcagc gtgcacgcgc gattctcggt cgagcagatc
     5881 gagaaggcgc gggccgagca cccggacgcc accgtgattg tccacccgga atgccgcgct
     5941 gaagtggttc aggctgccga catggacgga tccacggagt acatcattga catcatccgc
     6001 aacgcgccgt ctggcaccac gtgggcggtt ggtaccgaga tcaacctcgt tcgccgtctt
     6061 cagagccaga tgccggacaa gacgatcttc tgcctcgacc cggtcatctg cccgtgctcg
     6121 accatgtatc ggatccactc ggcgtacatc ctctgggcgt tggagcacct ggtggagggg
     6181 cgcgtggtca acgagatctc tgtggaaccg gacaccgcag aggcagcacg agtcgcgctg
     6241 gaccggatgc ttgcggtacc ctaacgatca tatccgtcga ttcgtgcagg gagcacgcaa
     6301 tgctgattca ggttggtgag caggcacccg acttcgagtt ggaggactcc tccggccaag
     6361 cgttccggct gtcggacatg cagggtctcg caaatgtcct gctgattttc tacccgaagg
     6421 acatgacgtc cggctgacgc gaccagcttg tcgccgtgcg cgacaacttc acgtcactgc
     6481 agcgcaatga gattcagccg gttggcgtca accatggcga cgccgagagc caccgctcct
     6541 tccgcgagaa gtacaacttt cccttcgaac tgcttgtcga cgagaggctc aaggtcgctg
     6601 cggcctacgg cgcactcaac gagcagggca acggaatctc tcggtcagtc gtggtggtcg
     6661 gcaaggatgg caatgtcgtc ttctcggaat cgggcgcgcc ggcgatgccg agggtaatca
     6721 atgccgtcaa ggcgacggct gtcgcggacg actagcatca gggcaaacag cagcgaatca
     6781 cagaacgggc ggtccatcac ggaccgccca tttggctgtt ggcagtggcc ccctcgcggg
     6841 ccaacttcct aatgtaccca ctcgtcgtcg tagcggggtg tcatccagta gttgcggaca
     6901 tggaagtagc gacgcagtgc catctcgtcc tgccggctca gtggctgcga ggtgtcgtac
     6961 ctgggtgcgt cacacagctc cgcccgcgtg atgcggagca gtaccgggtc ctgatcggcg
     7021 tcgaccaact ccgcgggcac ggccgtcagg cgcttgccga acttcagtgc cgagccggtc
     7081 tgcacgacga tatagcgcgg gatgatgcgc cgcgactcga tccccgtcac gtgatcctcc
     7141 gaaagcttcg tttcatagag cacgtcggcg accttgccca ccggacggcc catcctgctc
     7201 accaccgtgc ggcctcgaat cgagcggacg aagcgcgcgt gctggtcagg ctcgtcgaga
     7261 ttgcgcagcg gcgcgaccgc gtcggtgatc accacagggc cgccggccat cgggccgg
//
LOCUS       DASYWL010000298         2148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_409925, whole genome shotgun sequence.
ACCESSION   DASYWL010000298 DASYWL010000000
VERSION     DASYWL010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409925"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(399..1019)
                     /locus_tag="VGR22_10480"
     CDS             complement(399..1019)
                     /locus_tag="VGR22_10480"
                     /inference="COORDINATES: protein motif:HMM:NF020908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HEV2129033.1"
                     /translation="MPLRREGDQIEEIESFDEWVHERLEEAIARGDFDNLPHKGKPIR
                     IERNPFQPELDLAFSRLKNAGMAPAWIELDREIRRMQETLDRWLRASADRLAAEAARI
                     RALEEVPVEAQPSPWQPWYRRWWPFRLFVVDPEGPGEDDLIVEWRRWDFERRRDREQY
                     LERAKQLDAKIQLFNNSVPRNLWHLEKLRFLPAKASQRFDTAVPKR"
     gene            complement(1130..>2148)
                     /locus_tag="VGR22_10485"
     CDS             complement(1130..>2148)
                     /locus_tag="VGR22_10485"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEV2129034.1"
                     /translation="ALQDVLASLEGGAWGMAYASGLAATQNLAYLLRPGDHVLLSSDS
                     YGGTFRLFDKVLRNYGIEMSLADLSNLDEARDAVQENTKMVWVESPTNPYLRIVDIAG
                     WAEALKGRDTWLVVDNTFTSPYLQRPLSLGADFVLHSTTKYLGGHSDVVGGAAVGNDP
                     EVQQILAFHQNAAGAVPGPMDCWLTLRGIKTLGVRMDRHSANAAKVAEWLQAQDEVET
                     VYYPGLPSHPNHEIAVRQMSGFSGMVSFTLKGGYEVAEKLVENTRVFTLAESLGGVES
                     LIEHPAKMTHASTAGTGAEVEDSLVRLSVGIEHVDDQIADLEQAMQAATRRQLIGASS
                     RHAD"
BASE COUNT          366 a          735 c          666 g          381 t
ORIGIN      
        1 ccacggcaac ttcgatctcg ccgcctacca gaactacctg gctggcaagc tgaccgacta
       61 cgaacatcct caggaggcga tcgacgaggc actccagcac ctccccgacg tctccggcgt
      121 cgctgcggac tagccgtcat cgcgtatgaa ggcccgggtg ggttcctcca ctcgggcctt
      181 cttggttgca tccactcgca gcccggtgcc tcaaatggcg ggttcgaatg gacgtcggcc
      241 agtgggccgc acggcttgtc tgacgatccg gtcgaagcac ctatgctgcc accaagtgag
      301 ccggccccgg cgagaccaag atcttacagg acaatgcgac atgtcagtcc cggtctacac
      361 gttgagttga gcagcgattg cttccagcca gcatcaggtc atcgcttcgg gaccgcggtg
      421 tcgaagcgct gcgaggcctt ggcgggcagg aagcgcagtt tctccagatg ccagagattg
      481 cgcggaacgg agttgttgaa gagctgaatc ttggcatcga gctgcttcgc ccgctcgagg
      541 tactgctccc gatcccgtcg gcgctcaaag tcccaccgac gccactcgac gatcaggtca
      601 tcctcgccgg ggccctcggg gtcaaccacg aaaagccgga acggccacca gcgtcggtac
      661 cacggctgcc agggggaggg ctgcgcctcc accgggactt cctcaagcgc ccggatccgc
      721 gccgcctccg ccgccagacg gtccgcgctc gctcgcagcc accgatccag cgtctcctgc
      781 atgcgccgga tctcgcggtc gagctcgatc cacgccggcg ccatcccggc gttcttcagc
      841 cggctgaacg ccagatccag ctccggctgg aacgggttgc gctcgatcct gatcggcttg
      901 cccttatgcg gcaggttgtc gaaatcaccg cgggcgatcg cctcctcgag ccgttcgtgc
      961 acccactcgt cgaagctctc gatctcctcg atctgatcgc cttctcgccg gagcggcata
     1021 gtccttatcc tcgtacatgc gcctcaataa tcaccgacgc tcagcatagc aagtgcccct
     1081 gtcccgacag acaggggcac ggtgacgacg gttacgctcg tcgggaatgc tagtcggcgt
     1141 gccgggacga cgcaccgatc agctgccggc gggtcgccgc ctgcatcgcc tgctcgagat
     1201 cggcgatctg atcgtcaaca tgctcgatgc cgaccgagag tcggacgagc gagtcctcga
     1261 cctcggcgcc ggtccccgcc gtgctggcat gagtcatctt cgcggggtgc tcgatcagcg
     1321 actccacacc cccgaggctc tctgcgagcg tgaagacgcg cgtgttctcg accagcttct
     1381 cggcaacctc gtacccgccc ttcagtgtga aggagaccat gccggagaag ccggacatct
     1441 gccgcacggc gatctcgtgg ttcggatgcg acgggagacc ggggtagtag accgtttcca
     1501 cctcatcctg cgcctgcagc cattcggcaa ccttggctgc gttcgcgctg tggcggtcca
     1561 tgcgcacgcc cagcgtcttg atgccgcgca gcgtcaacca gcagtccatc ggtcccggca
     1621 ccgcaccggc cgcgttctga tggaaagcga gaatctgctg cacctcgggg tcgttgccaa
     1681 ccgccgcgcc accgacgacg tcggagtgcc cgccgaggta cttggtcgtg ctgtggagca
     1741 cgaagtccgc accgaggctg agcggccgct gtaggtaggg cgaggtgaag gtgttgtcaa
     1801 ccaccagcca ggtgtctcgg cccttcagcg cctctgccca gccggcaatg tcgacgatgc
     1861 gcaggtacgg gttagtcggg ctctcgaccc agaccatctt cgtgttctcc tgcacagcgt
     1921 cgcgcgcctc gtcgaggttg gagaggtcgg ccagcgacat ctcgatgccg tagttgcgga
     1981 gtaccttgtc gaagaggcgg aaggtaccac cgtagctgtc gctggagagc aacacgtgat
     2041 cgcccgggcg cagcaggtag gcaagattct gcgtcgcggc gagcccgctg gcgtaggcca
     2101 tgccccacgc gccgccctcg agcgaggcga gcacatcctg caatgcag
//
LOCUS       DASYWL010000299         2802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_616191, whole genome shotgun sequence.
ACCESSION   DASYWL010000299 DASYWL010000000
VERSION     DASYWL010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616191"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1258)
                     /locus_tag="VGR22_10490"
     CDS             complement(<1..1258)
                     /locus_tag="VGR22_10490"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2129035.1"
                     /translation="METEPSGFGERLRRRRLAAGLTQEELAARAGLTAKGVAALESGR
                     RRRPYPQTVRALATALGLDDTERAALVGAPQPATPPPLADPGVPVLPAPPTSTIGRDA
                     EVEEVLALLGQDDRRLVTLTGPGGVGKTRLALVVARQAADLFPDGVVFVPLAALTDPG
                     LVVPTVMAHLNLAERMDHRLIDDLAAYLRGRHLLLVLDNLEHLLPAAADISTLLTTTP
                     ELTILATSRAPLRLQGEREVPVAPLTLPRLVHLPTREEVAAAEAVQLFVDRARDAAPT
                     FALSQENAAAVAAIARRLDGLPLALELAAARLRILSPTELLARLDQSLPVLTGGARDL
                     PDRQRTIRQAIEWSYRLLAPEEQALFRRLSVFAGGWDAAAAEAVGGQDAATLGVLSAL
                     VEHSLVIAEASGDGSTRYRMLETIRQF"
     gene            1567..1956
                     /locus_tag="VGR22_10495"
     CDS             1567..1956
                     /locus_tag="VGR22_10495"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV2129036.1"
                     /translation="MAVFAVMERRGAGPPMHRHSRQDELIVVLEGRVTFYRAGERIDG
                     REGVCIVLPRGTEHGYVVESNAARLLIVVAPAERDFEACLAEMSRCTDEPVADLEQGE
                     LAIERLISTAAGFGVEITGPRPVGAGN"
BASE COUNT          507 a          883 c          975 g          437 t
ORIGIN      
        1 cgaactgccg gatcgtctcc agcatgcggt accgcgtgct gccatcgcct gacgcctcag
       61 cgatgacgag cgagtgttcc acgagcgcgg acaggacccc cagtgtggcg gcgtcttgtc
      121 cgccaacagc ctcggccgcc gcggcatccc agcccccggc gaagaccgac aaccgccgga
      181 acaacgcctg ctcctctgga gcgaggagcc ggtagctcca ttcgatcgcc tgccgaatgg
      241 tgcgctggcg gtcgggcagg tcccgagcac cgcccgtcaa cacgggaagg gattgatcga
      301 gccgggccaa cagttcggtg ggactgagga tccgcagccg agctgccgcg agctccagcg
      361 ccagcggcaa cccgtccagg cgccgagcga tggcagcaac ggccgccgcg ttctcctggg
      421 agagggcaaa ggtgggcgct gcatctcgtg cccgatcgac gaagagctgc actgcctcgg
      481 ccgcggccac ctcctcgcga gtcggcagat gcacgagccg gggcagggtg agcggtgcca
      541 ccgggacctc acgctccccc tgaagccgca agggcgcccg gctcgtggcg aggatcgtga
      601 gttcaggagt ggtggtgagc agggtggaga tatccgcggc ggcgggcagc aggtgctcga
      661 ggttgtcgag gaccagcagc aggtgccgcc cccgcaggta ggcagcgagg tcatcgatca
      721 gccggtggtc catccgctcg gcgagattga gatgcgccat cacggtcggg acgaccaggc
      781 ccgggtcggt gagcgcggcc agcggcacga agacgacccc gtcggggaag aggtcagcgg
      841 cttggcgggc gacgacgagc gccaggcgcg tcttgccgac acctccgggg ccggtgagcg
      901 tgacgagccg gcgatcgtcc tgcccgagga gagccagcac ctcctccact tccgcgtcgc
      961 ggccaatggt ggaggtgggc ggagcgggca gcacgggtac cccaggatcg gccaaaggtg
     1021 gcggggtggc cggctgggga gcaccgacca gcgcagcgcg ctccgtgtcg tccaggccga
     1081 gggcggtagc gagcgcgcgg acggtctggg ggtaggggcg gcgacggcgc ccgctctcca
     1141 gtgcggcgac acccttagct gtcagcccgg cccgggctgc aagctcctcc tgggtcaggc
     1201 cggccgcaag ccgtcgccgc cgcagccgct ccccaaaccc agatggttct gtctccatca
     1261 cggccgccat tcctccgtcc ggattgtgcc agccttgaga catcatctta caccgtttac
     1321 accccatcaa cgatgcagaa ctgtgagggt gaatgtgagg gtactgtcgg ttccattcgc
     1381 tcagcgcggc atccaggctg tagtacggat ccggtacgtg gatccaccac acgcattact
     1441 ggagaggtat ggacgatgca atcacagatt ggcacacacc aatcgatcga taatactccg
     1501 acagacagag agagtccggc aacgggcgtc agggacttgt ccatagctcg agcgacggag
     1561 tggcggatgg cggtcttcgc ggtgatggag cggcgaggcg ccgggccgcc gatgcaccgg
     1621 catagccgac aagacgagtt gatcgtcgtc ctggagggcc gggtgacctt ctatcgtgcg
     1681 ggcgagcgca tcgacggccg agagggcgtc tgtatcgtgc tgccgcgagg gaccgagcac
     1741 gggtacgtcg tcgaatccaa cgcggcgcga ctgctcatcg tagtagcgcc ggccgagcgg
     1801 gatttcgagg cgtgtctggc agagatgagc cgctgcaccg atgaacctgt cgcagacctg
     1861 gaacaaggcg agctggcgat cgagcggttg atctctactg ccgcggggtt tggtgtcgag
     1921 atcacggggc ctcgaccggt cggcgctgga aactgagacc gtcagtggtt ggggcgccca
     1981 tcgttgccag agccatggac tcgacgggga agccgcttcc ccccgcaccg gcgcctccca
     2041 cttggtgttt ctcagacatc caataggacg cgagccagta tccatcgcct tctgccgtca
     2101 gtggccgtcg ctggtcccaa catgatagga ccggcaacgt ttgacttgct cgacaccacc
     2161 tcggtccagg tcagtgcctg cattcaaggc acgctgctca tgcccgagtc gtagccattg
     2221 ctcaaccttg ccgggcggga gcgggccggc tggtaacacc gcgcgcctgg tagcaggtat
     2281 ccagcctcct ccgactggac gtccgtccct ggtcgtgtgc ggtatttctc gaaccagtca
     2341 cattcacatg acgccgacga tcaccaccga gatcatcgag cgaggccgcg gaaggccagc
     2401 ctcgtgcaca tgcgttccag ggatcaccat gcccgaccgg agcgtgaatg gagggggaag
     2461 aggccacgct ccggtgggca ggtgcgggga tgagggagcg ccacgccagg caccatttgt
     2521 ggctaagcca tggcggggaa gttggattcg tcgccagtgg tttcggtcca gcgtcggtgc
     2581 tgggtgcttt cacaccttca ctctgccgag cgacgcactg tcacgcatcg gtagatctgc
     2641 gtaaaaacca cgtcgaagcg aagggatggt gagcgacggg atgcggtagg cccattaggc
     2701 agattcaccg atgtgcggga cgagcggcat cgacagaatg ggatcagtgg cacgggccac
     2761 gactcacccg gcaccagtgc cggaaccaat gcagaaagcg ag
//
LOCUS       DASYWL010000300        18127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_653515, whole genome shotgun sequence.
ACCESSION   DASYWL010000300 DASYWL010000000
VERSION     DASYWL010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 18127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18127
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653515"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VGR22_10500"
     CDS             complement(<1..894)
                     /locus_tag="VGR22_10500"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV2129037.1"
                     /translation="MATETATWHERVSRDAIVDTARSLIAIPSVSGNEKDVMDFVVRW
                     CEERGINTTVTAKDPDRPNVIAWVGDPESGPVIAMNGHLDIVPVSDPKSWNSDPFDPV
                     VSEDGKKLYGRGASDMKSSVGVMLTMLDLFRNASLEGALMAHIVSDEELSANYGTLHV
                     LDEIDAGHLPRPDYVFIGEGSQLKVRNAERGILAFRVTVIGKASHTAVARVSGINAIA
                     KAARAVLELEGDINKFHPSIGHPVISINTIEGGVAHNVVPGDCTFTIDRRLIPGETRE
                     SVTEEIRQILDAIAAEDPNFRY"
     gene            complement(1006..1335)
                     /locus_tag="VGR22_10505"
     CDS             complement(1006..1335)
                     /locus_tag="VGR22_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEV2129038.1"
                     /translation="MKLIIAIVQDEDTDAVTEALIEAGYRFTKISTTGSFLRTGNTSL
                     MIGVEDEGVQGVMNILRRTARRRTQIAVPYSPALEPGLLYMPENFEVEVGGAVVFVAN
                     VSRFERI"
     gene            complement(1507..2325)
                     /locus_tag="VGR22_10510"
     CDS             complement(1507..2325)
                     /locus_tag="VGR22_10510"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEV2129039.1"
                     /translation="MEINLIVEAHAFAVDLARETGAMVRQRFSRARDIEHKGTIDIVT
                     DADRAAETMIATAIRDRFPEHRLLAEEGTTAAEDGSSPYRWVVDPLDGTTNFAHHYPH
                     FAVCIGLERENELLVGAVYEPMRDEMFEAAKGRGARLNGKPIGVTQRSELITSLLASG
                     FPYDLSAREVSNRLWIRFNDQCQGVRRDGAAALDIVYVAAGRLDGYWERPVMPWDKTA
                     ACLVAMEAGATVTGLAGEPFDVDDLGVTVANPELHAVMVSEIVAELEEIEASVQ"
     gene            complement(2577..3701)
                     /gene="tsaD"
                     /locus_tag="VGR22_10515"
     CDS             complement(2577..3701)
                     /gene="tsaD"
                     /locus_tag="VGR22_10515"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEV2129040.1"
                     /translation="MTIALPDNPVILGLETSCDETAAAVVVDGREVRSNVISSQIALH
                     QSHGGVVPELAARGQITAISPVVDAALREAEVSPSDLDAIAVTQGPGLAGSLLVGLNA
                     AKALAMGWKLPLIPVNHLEAHVYASWLQQPDADAIEPAFPSVALLVSGGHTALILMKS
                     HSEGKLLGQTIDDAAGEAFDKGARLLGLGYPGGPAIQRAAEGGDRNTIPLPRAWLDRS
                     LDFSFSGLKTSLLRHAQPWRLPDPPRELPPGTIFPPHQPPVFKDDFPVADFAASFEEA
                     IVDVLTIKAVWALERTGARSVVIAGGVAANRALRERLRAMVAETFGERHPTVSWPDLS
                     FCTDNAAMIAGLARWREPLTGGAAWSLDAYPNLAFGEKRG"
     gene            complement(3698..4345)
                     /gene="rimI"
                     /locus_tag="VGR22_10520"
     CDS             complement(3698..4345)
                     /gene="rimI"
                     /locus_tag="VGR22_10520"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEV2129041.1"
                     /translation="MYVIDEMTLDDIEEVHAIETRSFPSAWPLSAYRRELRNRRQSYY
                     VVLREDPDAPTELTTVARRFETDDEDGGKLQFLPFLRRRGGPPRGTIRGFAGMWILLD
                     EAHITTIGVDPDHQGQGLGELLFVALMDEAIQRGATWVTLEVRVSNQAAQALYRKYGF
                     TVQGRRPRYYSDNNEDAYIMWSESLKDDAYRERMKGLRAEALRRVSERMRLELAE"
     gene            4462..4545
                     /locus_tag="VGR22_10525"
     tRNA            4462..4545
                     /locus_tag="VGR22_10525"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4495..4497,aa:Leu,seq:caa)
     gene            complement(4553..5254)
                     /locus_tag="VGR22_10530"
     CDS             complement(4553..5254)
                     /locus_tag="VGR22_10530"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEV2129042.1"
                     /translation="MMDSGDTQPLAPIHSHPTSPTRARIKPRSPARRRHGARWFVSIA
                     LTLMMVIVLGFCALVTLIYWQARTDEARPVDAIVVMGAAQYNGRPSPVFRARLQHALD
                     LYNAGLAPLIVVTGGKAPDDEFTEGAAAQAWLAERGVPPEVILVENEGRSTWESVQGI
                     PSVFSSERHTRVLAVSDGFHLFRSELMLRELGYSAYSSAAPGSPIDPWSPVEFWYVIR
                     EAAAVLAFLPETLFD"
     gene            complement(5487..6644)
                     /locus_tag="VGR22_10535"
     CDS             complement(5487..6644)
                     /locus_tag="VGR22_10535"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HEV2129043.1"
                     /translation="MDERLRREQLHGTEMTEAEARIPERRSKERSQYALECGLPSAMS
                     VRDRTIPLFNRSYRTWAHHGTYMNVPFQEDMRELGLPDVAFVGAPLDAGTTFRSGTRF
                     GPQAIRQMSALPSGYNVEMGVDLHESLDMVDVGDVNIIPANIEKSFDQIDKAVAYLHE
                     HAIFPVVLGGDHSIGYPDIRAMSPYIDGNIGIIHFDRHSDISEYNIDERMHGTPFFHA
                     TNIPNAPATNLVQIGIGGWLGVHGGVRVARERMASCITLTDIDRYGIDRTCELALELA
                     WQDAEAVFLSFDVDSIDPAFAPGTGTPEAGGLLPREALAMLRLLCREGIAGMEVVEVS
                     PPYDHADITSVLAVRIINDVLGILVSEGKLGRMPETDDEEGVEGTRPDADA"
     gene            complement(6864..8861)
                     /locus_tag="VGR22_10540"
     CDS             complement(6864..8861)
                     /locus_tag="VGR22_10540"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV2129044.1"
                     /translation="MSDEAQPQQLAITAEEAIAGIAWRPGATTLQRSVLQRLIERTQS
                     GTYQSLLGRIAADPEWYWREVLADLDLAWQREPERIWNLDRGPAWPEWFPGGRFNLVE
                     SAVDRWVEREDGHRTAVIAEAEGGSVEKLTYRELRDEVIRVASGLRWAGVQPGDRVGI
                     YLPMITETVVAMLALARIQAVSVPLFSGYAEEAIVTRLRDSGATALVTADACQRRGKT
                     VPLKSVADDALIHVPGVQLCVVVRHEGDACRMVEGRDRWWHELADVADTDPSVPETPA
                     DTPFMIMYTSGTTGRPKGALHIHAGFPIKATHDLAYCFELQQDDVLFWLTDLGWMMGP
                     WLILGGLMRGSTIVLLEGVPDYPGPGRLWEMVERHRITVVGVAPTAIRALIPAGTRPV
                     EATDRSSLRALGSTGETWNPDPWWWFFDVVGERRCPIINYSGGTETSGGIVSSISILP
                     QRSCSFNGPVPGMVADVVNEHGESMRAGVGELVLRAPWVGMTNGFWQDPARYEETYWA
                     RFPGMWVHGDWAEVDEDGFWYIRGRSDDTMNIAGKRIGPAEIESAIVAEGSAREAAAI
                     AIPHPVKGSAIAVVAVLRTGVENSVECRSAMRDAVAERLGKPFRPEHVVCVEALPRTR
                     NAKIMRRMIRAAWLGEDPGDASALENPEAVDAIREAASRAT"
     gene            8996..9895
                     /locus_tag="VGR22_10545"
     CDS             8996..9895
                     /locus_tag="VGR22_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129045.1"
                     /translation="MVWRRLFHRQPNDEQRPAAQPDERTLPPHLKEQLKQRPRRTGDA
                     ADLRRRLAALRKRRTDALYDIEQGELALEDDNPWKQRIELLGETLETVEADYAEAEEV
                     EPGPYHPLPETPIEDMRVAIENDVATVSFRVGDEPFAYEEPLDWAERGHQIARTELRR
                     TAGDPSTVVPDNVPDELRDELHSHLENSLFVLATVLRDATLDEEPVPEKVTLADLARP
                     CSTCGGWMDVRGRCQHCARRKLRLQELFRERDRLLSERAAEIEEQHRMAERLPLARRR
                     LNDVEAEIAQIEEQLAAADQDAR"
     gene            complement(9997..10182)
                     /gene="rpsU"
                     /locus_tag="VGR22_10550"
     CDS             complement(9997..10182)
                     /gene="rpsU"
                     /locus_tag="VGR22_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEV2129046.1"
                     /translation="MRVEIRDNEGFEQLLRRFNKGIERSGVLREHRRGLRFISVQEEN
                     RAKRRKAERRRRRNQNK"
     gene            10333..11433
                     /locus_tag="VGR22_10555"
     CDS             10333..11433
                     /locus_tag="VGR22_10555"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEV2129047.1"
                     /translation="MSLPTRSRGIAVYPDRPGAIHRTEVEVPTVGPNDVLIETVRVGV
                     CGTDREIIHGTIGAPQEGADELVLGHELLGRVRAIGDAVHDLAEGSFVTATVRRPDSC
                     PACRAGQPDMCVWGKYEERGIVGLHGFMQEHIVERRDDVIPVPDELEPVGVLVEPLTV
                     IEKSLRHVDLVQSARMPWWEAKTALVLGAGPIGILGTMLLRSRGVEVITAARSEQPNL
                     SASVIEGAGATYLSTREASLDSAAKRLGQIDVIIDCSGASGVGFDAMRLLGVNGALVL
                     LSINAGGHRVEIPADAINNGIVAGNKTIIGIVNAGQEDFASAVQHLGRFEELWPGLLS
                     RMFTARIPFDGDVESIIEKSPGSIKTLIEFGG"
     gene            complement(11574..12707)
                     /locus_tag="VGR22_10560"
     CDS             complement(11574..12707)
                     /locus_tag="VGR22_10560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2129048.1"
                     /translation="MDRIVVLGGGVLGASAAFHLAGEGVDVTLIDRHDRGHATAAGAG
                     IIAPGTSLRDLPAFYSLAADAVRYYPELIGVLNDLEAGDTRYEVVGYLFPALTDDEHA
                     RLDEIQVMIERRYRAGMPNLGEVRRIDAREARERFPAIGDVAGAIFIPEAARVDGDYL
                     RRALMNAAARRGAHVIDGEARLVSATETSVEIEVSGDRLNVDRVIVAPGAWSNSVLEP
                     LGVSLPLHPQKGQIMHLVMEGADTSAWPIIGGFGSQYILTFGPDRVVCGATRETGSGF
                     DLRVTAGGLHSVTTEALRVAPGLAGATLVEVRVGLRPLSDDGYPFIDRVPGHEAVVVS
                     TGHGPSGLQLGPWSGRLAAELATGKAPSSDISAFRIGREMPVV"
     gene            13020..14999
                     /locus_tag="VGR22_10565"
     CDS             13020..14999
                     /locus_tag="VGR22_10565"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2129049.1"
                     /translation="MLRWSLRTRFLLFAVATLIPFAAVLIYFIDRDFDQRVAQSYASD
                     STLASSVTRSTTSFLSANAEALEGIAQLPSVVALDPENAEIQIGAARRLRPEFSGIFL
                     LDADQNLVTFSGTDPADLAAYISNRVDQAVSQNTVTFSQPIPLGGDATGIVMIVPVVT
                     IQTTETAVTESAPEGETETDEPAAPEPTPAAAGDQTTQPPGQTLGVIGGIIQTDRIAQ
                     SILPSTRGNAEIALVSADNIIVATGGIDPAASQLIQENMDAILLALEGELTQFDVVDA
                     NGNERTAAAAPVTFESAPWAAVVTRPMPELFDGGATIEVVIVVSLSLVVVFALAVVLS
                     ELTARPIRTMARRAYELNQGMLREPIVPVGGGEIRALSEEFAEMADHTANQVAWLEEH
                     RHDRERQAAQMRDLLRRTNRLQEGERRRIAGEIHDAVSPLITGALYQARALLMSNGST
                     SEEEIDSSLKSVSELLERATNELHDVIFELRPPDLDDLGLVAAIEAYVQTIQRTGLSC
                     RLEASGDPPNFTPEVRLSFYRIAQEALHNVVRHAGADEAVVRLETTDELVRMTVRDNG
                     AGFDPAQAVQPASLGLLSMRERAESIGATFTILTRPGGGTAIVVERPNSGDVMSDEVL
                     ESMMLMSSGDQPEEPTDKAGSEVAAMEPETTEPVR"
     gene            15013..15648
                     /locus_tag="VGR22_10570"
     CDS             15013..15648
                     /locus_tag="VGR22_10570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2129050.1"
                     /translation="MISVLLVDDHQIVIEGLRKLLGAAGDIEVTAEAHDAASALELTR
                     KFNPDVVLLDLRMPGATGIQATRRLRQQEYGGAIIVLTSYGDKAYVRQALEAGADGYL
                     LKNTPSTELIASIRSAARGRRQLSPELLDGVLEEFGGLAREKTVRDADLSDEDLEILS
                     RASQGATNREIGMAMNRSEIAIKKRLQSIFQKLEVNDRAHAVAEALRRGLI"
     gene            15802..18108
                     /locus_tag="VGR22_10575"
     CDS             15802..18108
                     /locus_tag="VGR22_10575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HEV2129051.1"
                     /translation="MDWFLPTLTWYLATTIAAFAVAPVLALLFRHVTDHGASLLRPIS
                     LLLVAWPVWLLASLGDGIVAFDGPALWITTAILALICWPIGRLTGVWRRDTLLHYGIA
                     EAGYVALFSIFVLLRGFVPAVGGPSTIDQEKPMDLMMLASSMRAESMPPLDTWLAGET
                     INYYYLGYTLWASVGEMIGTTPAVTYNLAVVSIIAMTIVAVVGTVANMLSRFAGMIAS
                     RIGGLLAAVLVIGIGTPWAAWQIAQDPSGQWQRFPFDGVMWNATRIIPVEGTGFAISE
                     FPAFTFLFADLHPHMMAIPFAITALGITWMLASLPPHQPSILRAAQLALAGLVGAGLY
                     AINSWDFPTWFAVIAGGILISSGFRTVTSRLAAIATALVAGLVAWSPFIVSFEAPVRT
                     PNTDFAEVVSGIPVIGDVLASLAAYSGDRTTVQDWLSIFGFFYPVLVAAIIVALLRSD
                     EQESDGMITTLVLLSGGLAVAIGVLTPVPLLILLCPPLLAGLLVILRSTTITLELLIV
                     GLASLSLLLALVPEFFYLYDIFGNRMNTIFKLYLQSWLMAGVATALALVWLWQRARAW
                     LPSRAIVMVSTVVIVLAGLAYPVVGASQWVQFKNPDTDWEGMDALTWLETSAAADPAT
                     ANALAWLWEHGTNDDVLLAAGGCAYRAPIGFPAAASGIPAIIGWDNHERQWRLSDETI
                     GAEIGGRVTDVIDLFARPTDDLLDEYGVTLLYIGRPETEGVPGVDPGPTCAPGPFPEA
                     TSPEFPGPGWAEVFSEDGVRILRRDTDA"
BASE COUNT         3192 a         6236 c         5622 g         3077 t
ORIGIN      
        1 gtaccggaag ttcgggtcct ccgccgcgat tgcgtcgaga atctggcgga tctcctccgt
       61 gacgctctcc cgggtttcgc cggggatcag gcgccggtcg atggtgaatg tgcagtcacc
      121 gggcacgacg ttgtgcgcga cgccgccctc gatggtgttg atgctgatta ccgggtgccc
      181 gatcgaggga tggaacttgt tgatgtcgcc ctcgagctcc agcaccgccc gtgcggcctt
      241 ggcgatggcg ttgatgcccg acacgcgcgc gaccgccgtg tgcgacgcct tgccaatcac
      301 cgtcacgcgg aatgccaaga tgccacgctc cgcattgcgc accttcagct ggctgccctc
      361 cccgatgaag acgtagtccg gtcgcggcag gtggcccgca tcgatctcat cgagcacgtg
      421 cagcgtgccg tagttggcgc tcaactcctc gtcgctgacg atgtgcgcca ttagcgcccc
      481 ctccaggctg gcgttcctga agaggtccag catcgtcagc atcacgccga cagagctctt
      541 catgtccgag gccccgcgcc cgtagagctt cttgccgtcc tcggaaacca cgggatcgaa
      601 gggatcgctg ttccagctct tgggatcgga caccggtacg atgtcgaggt ggccattcat
      661 cgcgatcacc ggtccgctct ccggatcacc gacccaggcg atcacgttcg gccggtccgg
      721 gtccttcgcg gtcacggtgg tattgatgcc gcgctcctca caccagcgga cgacgaagtc
      781 catcacatcc ttctcgttgc cgctcacgct cgggatcgcg atcagcgatc gggcagtatc
      841 gacaatcgca tcgcgggaga cgcgctcgtg ccaggttgcg gtttccgtcg ccatgggtct
      901 gtcctctcgc ggtaccttgc cagcatcacg ccagcgctta caacaacgct gccccgagca
      961 cacccggggc agcgccactg ttcatcagat cgtgagatcg ggaagtcaaa tgcgttcgaa
     1021 ccgggacacg ttcgccacga agaccaccgc accgcccacc tcgacctcga agttctcggg
     1081 catgtagagc aacccgggct cgagggcggg agagtagggc accgcgatct gtgtccgtcg
     1141 ccgggccgtg cgccgcagaa tgttcatcac tccctgcacc ccctcatcct cgaccccgat
     1201 catcaggctc gtgttgcccg ttcgcaggaa cgacccggtc gtgctgatct tcgtgaagcg
     1261 gtaccctgcc tcgatcagtg cctcggtgac tgcgtcggta tcctcatctt ggacgatggc
     1321 aatgatgagc ttcatcggcg ctatcctcct cccactcgcc ccggacaaga cgccggaagg
     1381 atgtcggggt cgcgtctcga gccgtttcat cgacttcaac tcgccgaatc ttccgagcaa
     1441 taacgtggca acagcgatcg caaacggcaa acgaatgtgc gatgagtcta ccagaagcgg
     1501 cggacgttac tggacgctcg cctcgatctc ctcgagctcg gcgacgatct ccgacaccat
     1561 cacggcgtgc agctcggggt tcgccaccgt cacccccaga tcgtcgacgt cgaacggctc
     1621 gccggccagg ccagtcacgg ttgccccagc ttccattgcc acgagacagg cggcggtctt
     1681 gtcccacggc atcaccggcc gctcccagta gccgtcgagc cgcccagcgg cgacgtagac
     1741 aatgtcgagc gcagctgccc catcccgccg gaccccctga cactgatcgt tgaagcgaat
     1801 ccagaggcgg ttgctcacct cgcgcgcgct gagatcgtag gggaatccgc tggcgagcag
     1861 cgacgtgatc agctcgctcc gctgcgtgac accgatcggt ttcccgttga gccgcgcacc
     1921 ccgtcccttc gccgcctcga acatctcgtc ccgcatcggc tcgtatacag cgccgaccag
     1981 cagctcgttc tcgcgctcca gcccaatgca gaccgcgaag tgcgggtagt ggtgcgcgaa
     2041 gttggtagtg ccatcgagcg ggtcgaccac ccagcgatag gggctgctgc cgtcctcggc
     2101 ggccgttgtg ccctcctcgg ccagcaggcg gtgttccggg aagcgatccc gaatagctgt
     2161 ggcgatcatc gtctcggcgg ccctgtcggc gtcggtcacg atatcgatcg tgcccttgtg
     2221 ctcgatgtcc ctcgcccggg agaagcgctg tcgcaccatg gcgccggtct cccgcgccag
     2281 atcgacggca aatgcatgtg cctcgactat tagattgatc tccaacgatc gctccctact
     2341 ctgcacagtc ggtgtcattc tgagcgtagc gaagaatctc ccggcgcgca gcgccgctcc
     2401 attccgaaaa gccggcaatc ctgacgagcc ttggccagac caactgggga gaactcaacc
     2461 gcccagcaag cagggcgggg aatgctttgc ggcgctttag ggctacctac acccctcgtc
     2521 tgctcaggat gacacctcct gacctcagat gtctccaacc cagaagcacg tcgtcatcac
     2581 ccccgcttct caccaaacgc caagttcggg taggcgtcaa gggaccatgc cgcaccacca
     2641 gtcagcggtt cgcgccacct ggcaagtccg gcgatcatcg ccgcgttgtc ggtgcaaaac
     2701 gagaggtcgg gccacgacac cgttgggtgg cgctcgccga aggtctccgc aaccatcgcc
     2761 cgcaaccgct cgcgcagcgc ccggttggcc gccacgcccc ccgcgatcac caccgacctc
     2821 gcccccgtcc gctccagcgc ccagacagcc tttatcgtca ggacatcgac gatcgcctcc
     2881 tcaaacgatg cggcaaagtc agcgaccgga aagtcgtctt tgaagaccgg gggctggtgc
     2941 ggcggaaaga tcgtgcccgg cggcagctcg cgcggcgggt cgggtagccg ccacggctgc
     3001 gcgtgccgca gcaatgaggt cttgaggccg gaaaagctga agtccaacga gcggtcgagc
     3061 caggcgcgcg gaagcggaat cgtgtttcga tcaccgccct ccgccgccct ctggattgcc
     3121 ggaccaccag ggtaaccgag tccgagcagg cgtgcgccct tgtcgaacgc ctcgccggcg
     3181 gcatcgtcga tcgtctgccc cagcagcttg ccctccgagt ggctcttcat caggatcagg
     3241 gccgtgtgcc cgccagagac gagcaacgcc acactcggga aggccggctc gatggcgtcc
     3301 gcatcgggct gctggagcca gctcgcgtag acgtgggctt cgaggtggtt gaccggaatc
     3361 agcggcagct tccaccccat cgccagcgcc ttggcggcgt tgagtccgac caggagcgac
     3421 cctgccaggc ccggcccctg ggtcacggcg atcgcgtcga ggtcactggg cgacacttca
     3481 gcttcacgga gggcagcgtc gaccacgggc gagatcgccg tgatctgccc gcgcgctgca
     3541 agctccggca cgacgccacc gtgcgactgg tgcagcgcga tctgggatga gatgacgttt
     3601 gagcgcacct cgcgcccgtc gacaaccacg gcggccgccg tctcgtcgca cgaggtctcg
     3661 aggccgagaa tgaccgggtt gtccggcagc gcgatcgtca ctcagccagc tccagccgca
     3721 tccgctccga gacccggcgc agcgcttctg cccgcaatcc cttcatgcgc tcacggtagg
     3781 cgtcgtcctt gagcgactcc gaccacatga tgtaggcatc ctcgttgttg tcgctgtagt
     3841 agcgtgggcg ccgcccctga accgtgaagc cgtacttgcg atagagcgcc tgcgccgcct
     3901 ggttgctgac ccgcacctcg agcgtcaccc atgtcgcgcc ccgctggatt gcctcgtcca
     3961 tcagcgcgac aaagagcagc tcgccaagtc cctgcccctg atggtccggg tcgacgccga
     4021 tcgtggtaat atgcgcctca tcaagcagga tccacatacc ggcgaacccg cgaatcgtgc
     4081 cacgcggggg gccaccccgg cgccgcagaa agggcaggaa ctgcaacttg ccgccatcct
     4141 cgtcatccgt ctcgaagcgg cgggcaacgg tggtcagttc tgtcggggcg tccgggtctt
     4201 cccgcagcac gacgtagtag ctctggcgcc ggttgcgcaa ctcacgacga taggctgaga
     4261 gcggccatgc cgagggaaaa ctgcgagttt cgatcgcgtg gacctcttcg atatcgtcaa
     4321 gggtcatttc atcgatgacg tacacggctc ggaaacacct tgcgcgagcg acagatcgcg
     4381 gcatacccat gcgggcggtg cgtggcaggt ctcaaagcac ggacgatgcc acgtcggtgc
     4441 ggtacactct cctccggacc ggccggagtg gcggaacggc agacgcagcg gactcaaaat
     4501 ccgccggggc aacccgtggg agttcgaatc tcccctccgg caccacccag aatcaatcga
     4561 agagcgtctc gggaagaaag gcaagcaccg cagcggcctc ccgaatcacg taccagaatt
     4621 ccacgggaga ccacgggtcg atcggactgc cgggtgccgc tgaactgtag gccgaatagc
     4681 ccagctcgcg caacatcagc tccgaccgga agagatggaa gccatcgctg acggcgagta
     4741 cccgcgtgtg tctctcggac gaaaagacgc tggggatgcc ctgcacgctc tcccaggtcg
     4801 accgcccctc gttctcgacg agaatcacct ccggcgggac gccgcgctcc gccaaccatg
     4861 cctgcgccgc cgccccctcg gtgaactcgt catccggcgc tttgccaccg gtgacgacga
     4921 tcagcggagc aagaccggca ttgtagagat cgagcgcgtg ctgaagccgg gcgcggaaca
     4981 cgggcgatgg tcgaccattg tactgggcgg cgcccatcac cacgatcgca tcgaccgggc
     5041 gcgcctcgtc ggtgcgtgcc tgccagtaga taagggtcac gagtgcgcag aacccgagga
     5101 cgatgaccat catcagcgtc agcgcgatcg agacgaacca gcgggcacca tggcgacgcc
     5161 gagcgggcga ccggggcttg attcgagcgc gggttggcga ggtgggatga gagtggattg
     5221 gagccaacgg ctgggtgtcg cccgagtcca tcatgccgga agcatgacac cctcacgctt
     5281 cgctgtgcaa cgtctccagc gtgcagcgat cgcgcttggc cgtctcgcgg tgcaccccct
     5341 cggctccata gacaagccca ccgcagagaa ctcccccggt accgcacgag caactgaagt
     5401 tgatgtcgct ccaactgagg acccaaagcc ccatgtcgga aattctgact gcggctcagt
     5461 atgacgacag gtagatcaat cctgaatcag gcgtctgcat cagggcgggt gccttcgacg
     5521 ccctcctcgt cgtcggtctc cggcatccgg cccagcttgc cttcggacac caggattccc
     5581 agtacgtcgt tgatgatccg taccgccagc acagaggtga tatccgcgtg gtcatagggc
     5641 ggcgacacct cgaccacctc catccccgcg atgccctccc ggcagagcag ccgcagcatg
     5701 gccagcgcct cgcgtggcag caacccgccc gcctccggtg tgccggtgcc cggcgcgaac
     5761 gccggatcga tgctgtcgac gtcgaacgag aggaagaccg cctcggcgtc ctgccacgcg
     5821 agttccagcg ccagctcaca ggtccgatcg ataccgtagc ggtcgatgtc ggtcagggta
     5881 atgcaggagg ccatccgctc ccgcgcgacc cgcaccccac catgcacgcc cagccacccg
     5941 ccaatcccga tctgcacgag attggtcgcc ggcgcgttcg ggatgttggt cgcgtggaag
     6001 aagggcgtgc cgtgcatgcg ctcgtcgatg ttgtactccg aaatatcact gtgccgatcg
     6061 aagtggatga taccgatatt gccgtcgatg tagggtgaca tcgcgcggat atccgggtag
     6121 ccgatcgaat ggtcgccgcc gagcaccacc ggaaagatcg cgtgctcgtg caggtaggcg
     6181 accgccttgt cgatctggtc gaagctcttc tcgatgttcg ccgggatgat gttcacatcg
     6241 ccgacatcca ccatgtccag tgactcgtgg agatcgacgc ccatctcgac gttgtatccg
     6301 gaaggcagcg cggacatctg gcgtatcgcc tgcggaccga agcgggtgcc ggaacggaag
     6361 gtggtgccgg catcgagtgg tgctccgacg aacgcgacgt ccggcaaccc gagctcacgc
     6421 atatcctcct gaaatggaac attcatgtag gtaccgtggt gcgcccaagt gcggtaggag
     6481 cggttgaata gcggaatggt ccggtcgcgc acgctcatcg ccgagggcag cccgcactcg
     6541 agagcgtact gactacgctc cttcgaccgt cgctcgggaa tgcgcgcctc cgcctcggtc
     6601 atctcggtgc cgtgaagctg ttcccgccgc agtcgctcgt ccatgatgtc tctccagacc
     6661 ttgggccgtt ttccacgtcg ccgctgccac acagcctgtc atttcgaccc cttcgactcg
     6721 gctcagggca ggctcgcgcc cagagggtgc gaagtggaga aatctcttgc ccgccgaacg
     6781 cgggcaccat cgccgcgaga gatttcttga cacagctgca acgctggcac gcctttgcgg
     6841 ctcgaaatga cgggttcgcg accttacgtc gcgcgactcg ctgcttcccg aatggcgtca
     6901 accgcctccg gattctccaa ggcactggcg tcgccaggat cctcaccgag ccatgctgcc
     6961 cggatcatcc gccgcatgat cttcgcattc cgagtgcgcg gcaaggcctc cacgcacacg
     7021 acatgctcgg gccggaatgg cttgccgagc cgctcggcca ccgcgtcccg catggcgctg
     7081 cggcactcca cactgttctc gacgcccgtc cgcagcacgg cgaccaccgc aatggcgctg
     7141 cccttcaccg gatgcgggat cgcgatcgcg gcagcctcac gcgcgctgcc ctcggcgacg
     7201 atggcactct cgatctctgc cggaccgatg cgcttgccgg cgatgttcat ggtgtcgtcg
     7261 gatcggcccc ggatgtacca gaagccgtcc tcatcgacct cggcccagtc accgtggacc
     7321 cacattccgg ggaagcgcgc ccagtaggtc tcctcgtagc gcgccggatc ctgccagaag
     7381 ccgttcgtca tacccaccca cggggcgcgc agcaccagct caccaacccc ggcccgcatc
     7441 gactcgccgt gctcattgac gacatccgcc accatgcccg gcaccgggcc gttgaacgag
     7501 caggatcgtt gcggcaggat ggagatactg ctgacgatgc cgccggatgt ctcggtgcca
     7561 ccggagtagt tgatgatcgg gcaacgccgc tcgcccacga cgtcgaagaa ccaccaccac
     7621 ggatcaggat tccacgtctc cccggttgac ccgagcgcgc gcagcgaaga ccggtcggtg
     7681 gcctcgaccg gcctggtgcc agcagggatc agcgcccgga tcgcggtggg cgccacaccg
     7741 accacggtga tccggtgccg ctcgaccatc tcccagaggc gtccgggacc ggggtaatcc
     7801 ggcacgccct ccagcagcac gatcgtgctg ccgcgcatca gcccgccgag gatcaaccac
     7861 ggtcccatca tccagccgag atcggtcagc cagaagagga catcgtcctg ctgcagctca
     7921 aagcagtagg cgagatcgtg cgtcgcctta atcggaaatc cggcgtggat gtggagtgcc
     7981 cccttcggtc ggccggttgt gcccgaggtg tacatgatca tgaagggcgt atcagcaggc
     8041 gtctcgggga ccgatgggtc ggtatcggca acatcggcca gctcgtgcca ccagcggtcg
     8101 cgcccctcca ccatccggca agcgtcgccc tcatgccgca cgaccacgca aagctgcacg
     8161 ccgggcacgt gaatcagggc atcgtcggca acgctcttga gtggaacggt cttcccgcgc
     8221 cgctgacagg cgtcggcggt aacgagtgcc gtcgcgccgc tgtcccgcag ccgggtgacg
     8281 atcgcctcct cggcatagcc cgagaagagc ggcacgctga ccgcctggat gcgtgccaga
     8341 gccaacatcg cgaccaccgt ctcggtgatc atcggcaggt agataccgac tcgatcaccg
     8401 ggctgcactc cagcccaccg caggccactc gcgacacgga tgacttcgtc acgcagctcc
     8461 cggtaggtga gcttctcgac gctgccgcct tccgcctcgg cgatcacggc ggtgcgatga
     8521 ccgtcctccc gctccaccca gcggtcaacg gcgctctcca cgaggttgaa ccggccaccc
     8581 gggaaccatt cgggccatgc aggtccccgg tcgaggttcc agatccgctc cggctcccgc
     8641 tgccaggcaa ggtcgaggtc ggccagcacc tcgcgccagt accactccgg gtcagcggcg
     8701 atccggccta ggaggctctg gtaggtcccg ctctgcgtgc gttcgatcag gcgctggagc
     8761 acgctccgct gcagggtagt ggcacccggc cgccacgcga tgccagcaat cgcctcctcc
     8821 gcggtgatcg cgagttgctg cggttgtgcc tcgtcgctca caggccctcc aggacgctcc
     8881 ctaacagata acagctggtt gtccgtgatc tgggcttcat tgtggccaac cgccgggaca
     8941 gggtcaaggc gggcactgtg cgacacttgc cggggagacg agataaggag gacggatggt
     9001 ctggcgacga ctctttcacc gacagccaaa cgacgagcag cgcccggccg cacagcccga
     9061 cgagcggacg ctgccgcccc acctcaagga gcagctcaaa cagcgtcccc gccgtaccgg
     9121 tgacgcggct gatcttcgcc gtcgtcttgc cgcgctgcgg aagcgccgca ccgacgccct
     9181 ctacgacatc gagcagggcg agctcgcgct tgaggatgac aacccttgga agcagcgcat
     9241 cgagctactc ggcgagacat tggagaccgt cgaggccgac tacgcggagg ctgaggaggt
     9301 cgaacccgga ccgtaccatc cgctaccgga aacgccgatc gaggacatgc gcgtggcgat
     9361 cgagaacgat gtcgcgacgg tttccttccg agtcggcgat gagccgttcg cgtacgaaga
     9421 accgctcgac tgggccgagc ggggccacca gatcgcccgg accgagctac ggcgcactgc
     9481 tggcgatcct tcgacggtgg tcccggataa cgtaccggac gagttgcgcg acgaactgca
     9541 cagccacctg gagaactcgc ttttcgtgct ggcgaccgtg ttgcgggatg cgacgctcga
     9601 cgaggagcca gtgccggaga aggtcacgct ggccgatttg gctcgtccct gttcgacctg
     9661 cggtggctgg atggacgtcc gcgggcgctg ccagcactgt gcgcgccgca aacttcggct
     9721 gcaggagctc ttccgggagc gtgatcgcct gctgagcgag cgcgcagccg aaatcgagga
     9781 gcagcaccgc atggccgagc gcctgccgct ggcccgccgc cgcctcaacg acgtcgaagc
     9841 cgagattgcc cagatcgagg agcagctggc cgctgccgat caggacgcca ggtagggacg
     9901 gacctccacg cggctcaggc acaacgaaac aaccgccgcg ggcttccccg ggcggttgcg
     9961 tgcatgattc agcaggaaga gttgctggtc agcagattac ttgttctgat tgcggcggcg
    10021 gcggcgctca gccttacggc gctttgcgcg gttctcctct tggacagaga tgaaccggag
    10081 accccggcgg tgctcacgga gcacgccaga ccgctcaatt cccttgttga aacgacggag
    10141 cagctgctcg aagccttcgt tgtctcgaat ctcaacacgc atgttcgctc atcaccacct
    10201 ttatgcgcac gatggcagca caggcacagc ccaaaccgcc tacgaatgag gcatcgctag
    10261 tatacgctat gcagagtgtc gaattccatc ggctccggta tcgtcccaaa cccagcagga
    10321 gcattctcca caatgtctct cccgacccga tcccgcggca tcgccgtcta ccccgaccgc
    10381 cccggtgcca tccaccgaac cgaggtcgag gtgcccacgg ttgggccgaa tgacgtgctg
    10441 atcgagacag tccgtgttgg cgtctgcggc accgaccgcg agattatcca cggcaccatc
    10501 ggtgccccac aggagggcgc ggacgagctc gtcctcggcc acgagctgct gggacgggtg
    10561 cgggcgatcg gcgacgcggt ccacgacctt gccgagggca gcttcgtcac ggcaaccgtt
    10621 cgccggccgg atagctgccc agcctgccga gccggacagc ccgacatgtg cgtgtggggc
    10681 aagtacgagg agcgcggcat cgtcggcctg cacggcttca tgcaggaaca catcgtcgag
    10741 cgccgcgacg acgtgatccc cgtgcccgac gagctggaac cggtcggcgt gctggtcgaa
    10801 ccactcacag tgatcgagaa gtcactgcgc cacgtcgacc ttgtgcagag tgcgcgaatg
    10861 ccctggtggg aggcaaaaac ggcactcgtg ctcggcgccg ggccgatcgg tattctcggc
    10921 acaatgctcc tgcgatcacg cggagtcgag gtcatcaccg cggcccgctc ggaacagccg
    10981 aacctctcag caagcgtgat cgagggagct ggcgcaacct atctctccac ccgcgaagca
    11041 tcactcgatt ccgcggcgaa gcgccttggc cagatcgacg tcatcatcga ctgctccggt
    11101 gccagcggcg tcggcttcga cgcgatgcgg ctgcttggcg tcaacggcgc actggtgctg
    11161 ctgagcatca acgccggtgg gcacagggtc gagatcccgg cagacgcgat caacaacggc
    11221 atcgtcgcgg ggaacaagac gatcatcggc atcgtcaatg cgggccagga ggacttcgca
    11281 agcgccgtgc aacatctggg ccgcttcgag gagctttggc cggggctgct gtcgcggatg
    11341 ttcaccgccc gcattccgtt cgacggggac gtcgagtcga tcatcgagaa gtcgccgggc
    11401 agcatcaaga cgctgatcga gttcggcggc tgatatcgag cttgccgcgc cgcccgctta
    11461 cgcaggggct ggcacgagtg cctgtcctgt gcgccgaccg gaaacccgtg cgagtctacc
    11521 cctccgtgat gccagcaggc ccgctgattc agtgccgggc gccggagccg gcgctacacg
    11581 acgggcatct ctcggccgat gcgaaacgcg gagatgtccg acgaaggcgc cttgccggtg
    11641 gccagctccg ccgcgagcct gccagaccat ggtccgagct gcagccctga cggcccatgc
    11701 ccggtcgaga cgacgaccgc ctcatgtccc ggcacgcggt cgatgaacgg atacccgtca
    11761 tccgagagcg gccggagccc aacacgcacc tcgaccagcg tggcgccggc gagccccggc
    11821 gccacccgta gcgcctcggt cgtgaccgag tgcagcccgc ccgccgtgac gcgcagatcg
    11881 aacccggaac ccgtctcgcg cgtggccccg cagaccaccc ggtccggccc gaacgtgagg
    11941 atgtactggc tgccgaaccc accgatgatc ggccacgcgg acgtatcagc gccctccatg
    12001 acgaggtgca taatctggcc cttttgtggg tggaggggca gcgaaacacc gagcggctcc
    12061 agtaccgaat tggaccaggc accaggcgcc acgatcaccc gatccacgtt cagccggtcc
    12121 ccactgactt cgatctccac cgatgtctcc gtcgccgata cgagtcgcgc ctcaccgtcg
    12181 atcacgtgtg cgccccggcg tgccgccgcg ttcatcaacg cccgccgcag gtagtccccg
    12241 tccacgcgcg cagcctcggg gatgaaaatg gcgccggcga catcaccgat cgccgggaag
    12301 cgctcccgcg cttcgcgcgc atcgatgcga cgcacctcgc cgagattcgg catccccgca
    12361 cggtatcgtc gctcgatcat gacctggatc tcgtcgagcc gtgcatgctc atcatcggtc
    12421 agggccggaa agaggtaccc gaccacctcg tagcgtgtgt cgccggcctc gagatcattc
    12481 aggacaccga tcaactctgg gtagtagcgc accgcatccg ccgcaagcga gtaaaacgcc
    12541 ggaagatcac gcaagctcgt gccgggcgcg atgatacctg cccctgcggc cgtggcgtgg
    12601 ccgcgatcat gccgatcgat gagcgtgaca tcaacgcctt cgcccgctag gtgaaacgcg
    12661 gcgctggcgc ccagcacgcc gcccccgaga accacgatcc ggtccatgtc gctcctcctt
    12721 tcggtgcctc ttcgggcccg tccagctcaa gccgcaaggt cattcggata cccgcagggg
    12781 ttccgttgta tagggaaact gtgtgctcag agtggcagac tagatcggac ccgcgtcccg
    12841 tatgggccgg gtttgccctg caaagtattc acgtttctcc agaatggggc atactgtgcg
    12901 cacgaattct tatttcccga tcgcatcgcg ccggactcga ggtcatatgg acatcggcat
    12961 gtgcgcgcac ctactcaaga tcatcccgcg tccctcgctg ctggccgagg ggtaacggca
    13021 tgctgcgctg gtccctccgc accagattcc tgctgtttgc agtcgcgaca ctgattccgt
    13081 tcgcggccgt cctcatctat ttcatcgacc gcgatttcga ccaacgtgta gcgcaatcct
    13141 acgcctcgga cagcaccctg gcgagcagcg tgacccgttc aaccacctcg ttcctctccg
    13201 ccaatgccga ggcactcgag ggcatcgctc agttgccgag cgtcgtcgca ctcgatccag
    13261 aaaacgccga gatacagatc ggagccgcca gaagactgcg accggagttt tccggcatct
    13321 tcctgctcga tgccgatcag aacctcgtta ccttctccgg caccgatccc gcggatctgg
    13381 cagcttatat cagcaatcgg gtcgaccagg cggtcagtca gaacacggtc accttttccc
    13441 agcccatccc actcggaggt gacgcgaccg gtatcgtgat gatcgtgccc gtggtgacca
    13501 tccagacaac cgagacggcg gtcaccgaga gcgcgcccga gggcgagacg gagaccgacg
    13561 aaccggccgc cccggagccc acacctgccg ccgcgggaga tcaaaccacg cagccgcccg
    13621 gacaaacact cggcgtgatc gggggcatca tccagaccga tcgtatcgcc cagagcatcc
    13681 tcccctcgac ccgtggcaac gcggagatcg ctctggtcag cgccgacaac atcatcgtcg
    13741 cgaccggtgg gattgatccg gctgcctcac agctgatcca ggaaaacatg gatgcgattc
    13801 tgctggctct ggaaggcgag ctgacgcagt ttgacgtggt tgacgccaac ggcaacgagc
    13861 gaacggccgc tgcagcaccc gtcaccttcg agtcggcccc atgggctgcg gtcgtcacca
    13921 ggccgatgcc ggagctattc gacggtggtg ccaccatcga ggtggtcatc gtggttagcc
    13981 tcagcctggt cgtggtcttc gcgctcgccg tggtgctgag cgagctcacc gcacggccga
    14041 tccggaccat ggcgcgccga gcatatgagc tcaaccaagg gatgctcagg gagccaatcg
    14101 tgcccgtcgg cggcggcgag atccgggcac tcagcgaaga gttcgccgag atggccgacc
    14161 acaccgccaa tcaggtggcg tggctcgagg agcaccgcca cgatcgcgaa cgccaggcgg
    14221 cccagatgcg tgacctgcta cgccgcacga accgcctgca ggagggcgaa cgccgccgca
    14281 tcgctggcga gatccacgat gccgtctcgc cgctcatcac cggtgccctc taccaggctc
    14341 gcgccctgtt gatgagtaac ggctccacct cagaggagga gatcgattca tcactgaagt
    14401 cagtcagtga gctgctcgag cgcgccacca acgagctgca tgatgtcatc ttcgagctgc
    14461 gcccacctga cctcgacgat ctcggactcg tcgcggcgat cgaagcctac gtccagacca
    14521 tccagcggac cggtctctcc tgccggctgg aggcgagtgg cgacccgccg aactttacac
    14581 ccgaggtccg cctcagcttc taccgcatcg ctcaggaagc gctgcacaac gtggtgcgtc
    14641 atgccggcgc agatgaagca gtagtgcgac tggagacaac ggatgaactg gtgcgcatga
    14701 ccgtgcgcga caacggcgcc ggcttcgacc cggcgcaggc ggtgcaaccg gcgtcgctcg
    14761 gcctgctcag catgcgcgag cgagctgagt cgatcggcgc gacgttcacc atcctgaccc
    14821 ggcccggcgg tggcaccgcg atcgtcgtcg agcgcccgaa ctcgggcgat gtcatgtccg
    14881 acgaggtgct ggagagtatg atgttgatgt cgagtggcga tcaaccggag gagccaaccg
    14941 acaaggccgg cagcgaggtt gcagccatgg agccggaaac gaccgaaccg gtccgctaag
    15001 gaataagcat acgtgatttc agttctactc gtcgatgatc accagatcgt gatcgaaggc
    15061 ctgcgcaaac tgctcggcgc cgccggtgat atcgaggtca ccgccgaggc gcacgacgcc
    15121 gcgtccgccc tcgaactcac ccggaagttc aacccggacg tggtgctcct cgacctgcgc
    15181 atgccgggcg caaccggcat ccaggcaacc cgccgtctgc gccagcagga gtacggcggt
    15241 gcgatcatcg tactcacctc ctatggagac aaggcgtacg tccgccaggc gctcgaggcc
    15301 ggggcggacg gctacctgct caagaacacg ccgtcgaccg agttgattgc gtcgatccgc
    15361 tccgcggcgc gcggtcggcg gcaactcagt cccgaactgc tcgacggcgt gctggaggag
    15421 ttcggtgggc tcgccaggga gaaaaccgtg cgcgacgccg acctgagtga cgaggatctc
    15481 gagatcctct cccgggcgag ccaaggcgcc accaaccgcg agatcggcat ggcgatgaac
    15541 cgcagcgaga tcgccatcaa gaagcgcctg cagtcgatct tccagaaact ggaggtcaac
    15601 gaccgcgctc acgccgtcgc ggaggccctt cgtcgcggac tcatctagcc accggacccg
    15661 atcgacacac catcggaatg gcgccagccc gtcagcacct gcggtagcat tgcagatggg
    15721 ccgctgagcg ttccgtcacg caccgccgtt ctgccatgcc tatcgctcca gcctgatgcc
    15781 catccctgag gaagcccgat cttggactgg ttcctgccca cgcttacgtg gtacctggcc
    15841 acgactatcg ccgcgttcgc cgtcgccccg gtgctcgccc tgctcttccg gcacgtcacc
    15901 gaccatggcg ccagccttct ccgcccgatc agcctgctgc tcgtcgcctg gccggtctgg
    15961 ctgctcgcca gtcttgggga cggcatcgtc gcgttcgacg gaccggcgct gtggataacc
    16021 accgccatcc tcgcgctcat ctgctggccg atcggccgcc tcactggcgt gtggcgacgc
    16081 gacaccctgc tccactacgg catcgccgag gccgggtacg tggccctctt ctccatcttc
    16141 gtgctgctgc gtggattcgt cccagccgtc ggtgggccga gcaccatcga ccaggaaaag
    16201 ccgatggacc tgatgatgct cgcctcctcg atgcgggcgg agtcgatgcc tccactggac
    16261 acctggctcg ctggcgagac catcaactac tactacctcg gctacacgct ctgggcgagt
    16321 gtcggcgaga tgatcgggac cacccctgct gtcacctaca atctcgccgt cgtctcgatc
    16381 atcgcaatga ccatcgtggc agtggtcggc accgtggcaa atatgctttc acgcttcgcc
    16441 ggcatgatcg cctctcgtat cggcggtctg ctcgcagctg tgctggtgat cggcatcggc
    16501 acgccatggg cggcatggca gatcgcgcag gatccatccg gacaatggca gcgcttcccg
    16561 ttcgacggcg tgatgtggaa tgcgacgcgc atcatcccgg tggagggcac cgggttcgcg
    16621 atcagcgagt ttccggcctt cacctttctc ttcgccgacc tgcatccgca catgatggcg
    16681 atccccttcg cgatcaccgc gctcggcatc acctggatgc ttgccagcct gccgccacac
    16741 cagcccagta tcctccgcgc tgcgcaactg gcgctggcag gactcgtcgg agctggtctc
    16801 tacgccatca actcgtggga cttcccaacc tggttcgcgg tgatcgccgg cggcatcctg
    16861 atctcgtccg gcttccgaac ggtcacttcc cgactcgccg cgatcgcgac tgctctggtc
    16921 gccggcctcg ttgcctggtc gccattcatt gtcagcttcg aggcgccggt gcgcacgccg
    16981 aacaccgact tcgccgaagt tgtcagcggc atcccggtga tcggcgatgt gctggcctca
    17041 ttggctgcct attcaggtga tcggacgacg gtgcaggact ggctctccat cttcggcttc
    17101 ttctaccccg tgctggtcgc ggcgatcatt gtcgcgctgc tgagatccga cgagcaggag
    17161 tctgacggca tgatcacgac gctcgtcctg ctctccggag ggctcgccgt agcaataggg
    17221 gtgctcacac cggtgccgct gctgatcctg ctctgcccgc ccctgcttgc aggcttgctg
    17281 gtcatcctcc gttcgaccac catcacgctg gaactactga tcgtcggcct cgcctcactc
    17341 tcgttgctgc tggccctggt ccccgagttc ttctacctct acgacatctt cggcaaccgc
    17401 atgaacacga tcttcaagct ctatctgcag agctggctga tggccggagt cgccaccgca
    17461 ctggcgctcg tctggctctg gcagcgggcg cgggcatggc tcccgtcgcg tgcgatcgtc
    17521 atggtctcga ccgtggtgat cgtcctggcc gggctggcct acccggttgt cggtgcgagc
    17581 cagtgggtgc agttcaagaa tccggacacc gattgggagg gcatggacgc gctgacgtgg
    17641 ctggagacat cggccgccgc ggacccggcg accgccaacg cgctggcgtg gctctgggag
    17701 catgggacga acgacgatgt gctgttggca gcgggaggat gcgcctaccg ggcaccgatc
    17761 ggtttccccg cggcggcgag cgggatcccg gcgatcatcg ggtgggacaa ccacgagcgg
    17821 cagtggcgtc tgagtgacga gaccatcggc gccgaaatcg gggggcgtgt gacggacgtg
    17881 atcgacctct tcgcccgccc gaccgatgac ctgctggacg agtacggcgt gacgttgctc
    17941 tacatcggcc ggccagagac agagggcgtc ccaggtgtcg atccggggcc gacctgcgcg
    18001 cccggcccat tccccgaggc gacctccccg gagttccccg gccccggctg ggccgaggtc
    18061 ttcagcgagg acggcgtccg gatcctgagg cgggacacgg atgcgtgagg gcgcaacggc
    18121 cctgtgg
//
LOCUS       DASYWL010000301        20620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_447179, whole genome shotgun sequence.
ACCESSION   DASYWL010000301 DASYWL010000000
VERSION     DASYWL010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 20620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20620
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447179"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..2108)
                     /locus_tag="VGR22_10580"
     CDS             complement(180..2108)
                     /locus_tag="VGR22_10580"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEV2129052.1"
                     /translation="MPTPEPISFDRLADRQYPREPRISPDGTAVAMTVMYASKKDDRA
                     TSQIWLSRNHGEPFPFTGGKAGAADPGWSPDGSCLVFTSSRDDKDERAGLFVIPADGG
                     EAQQIGDVRGEIAKPRWSPDGSCIAFLMRDPETEAEKKDKEAKRDQVVVEEEPKHQRL
                     WVIDPDTGKRRCLTTGNRSVRDYAWAPDGQELVVITTELPTANSLFMPSRVARVPVAG
                     GLEHEITTFPTAPSTPVVRDVRGEQVVAVIDSDHRADPSPSIWIAPWDGGERRNILPE
                     LRGAVYDIVADPTTPDRVLAVIVEGVHGRLYSVSLETDRRELLSPGTMREHGSISAGI
                     SASRDGNHVAFVWTASDEPEEVWTIDRNGSTEKVTSFGKPFEGGLAPGEVVRWQSFDG
                     VEIEGVLVRPARAEGPLPLFVEVHGGPSWQWEDRVMLDWHDWAQMLATRGWAVLMPNP
                     RGSTGYGSAFEKLLQDDVGGGESKDLIAGAEAMVERGIADRDRLAIAGWSWGGYLTAR
                     TITQTRRFRAAVMGAGIGNLASDHGAGDIPHANTLYYPSHPYDEGTWEFYAKGTPIRE
                     ASQVATPTLILHGDSDDRVHPTQGQEFFRALQHAGVPVQFVRYPREEHSIREREHQID
                     LMERIVTWLNKWVLSPKR"
     gene            complement(2095..2667)
                     /locus_tag="VGR22_10585"
     CDS             complement(2095..2667)
                     /locus_tag="VGR22_10585"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2129053.1"
                     /translation="MSDDHVLRRSQASDAPAIHRIRAHPVTRRYQPLEQRSVEDLRLI
                     LDERGHAEVTPWQNGKLEWTVVVNGDVAGWVTVSVTSRSHHIGTIGYSIAPRFQARGI
                     ATRAVRDVAATALDPSGLALERLEAVAAVENIASRRVLEKCGFAFEGIARSYLIISGA
                     RIDHARYARLHTDVQGDDRTRDDGAPHAHA"
     gene            2886..5639
                     /gene="ppc"
                     /locus_tag="VGR22_10590"
     CDS             2886..5639
                     /gene="ppc"
                     /locus_tag="VGR22_10590"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF000584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEV2129054.1"
                     /translation="MAKDRGAAARPGDRRLSDDVYLLAGLLGEVITSMAGEDAFELEE
                     AARALAKRLRRGDESAGQQLEMLISESNTDDLRILIRAFTNYFQLINLAEDNERIRRV
                     RSRELASPNEPRRGSIRQAIQMMVDGGVDAEQMQHLLDHAQVRLVLTAHPTEARRRTV
                     IAKLARIFAIIRELDERHALPHEVTRARGLLASTIAELWSSNELRSVSPTVLDEVRGV
                     LVYFGSTLVEVIPLIYRDLEEALKDAYPDAEITVPPFITFGSWIGGDRDGNPFVTPDI
                     TEATLHIMRAAALALLDHRLTELAGRLSLSDAMVRPSDHLQPLMTRYARRFPELAAQL
                     RQQNADEPYRQLVILMRERLRATRDGGEHGYARGSELLDDLKHVAAALREQSAGTILG
                     GELHDVIRLVEVFGFEFVTLDIREHSKRHGQAFDEIFRAVRVEEAYLALPEEEKQALL
                     VREINDPRPLVPVNLEVLPETARQVIETFRMIWRVRHHGYPDAIETYIISGTDAPSDM
                     LEVLLFMKETRLASDGGHHASLRIAPLFEEGATLAASRETMATVLGYPEYRAALESAG
                     GVQEIMIGYSDSNKDVGYLASSWRLHQAQRRLAHFLEEQGIEYLFFHGRGGSIGRGGG
                     PTNVAIMALPSGTLNGRIKITEQGEVISARYSMAQIAHRELELTVGAILAHAVGGDRI
                     TDPGRRERYESVMERMAEVSAEFYRDLVYGDPDFGTFFHQATPIDAIARLQLGSRPAK
                     RVQSDRIEDLRAIPWVFSWTQSRIILPAWYGLGTALEDAIERHGLDLLQEMARGWSFF
                     TALLSNAEMGLAKADLTVGHRYVELVEDAVVRDRIWGRISDEFARSVQHLTAVRGEEL
                     LLDHDSVLQRSVERRNPYVDPLSYIQVELLERLREQPIDEDVLQTLHLAVNGIAGGLK
                     NTG"
     gene            5636..8098
                     /gene="priA"
                     /locus_tag="VGR22_10595"
     CDS             5636..8098
                     /gene="priA"
                     /locus_tag="VGR22_10595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEV2129055.1"
                     /translation="MIATATTLHADVAVDGAGHATNGGVLTYTVPECLAGLLEPRQLV
                     WVPLRSKLVLGIVSRVHDESPRFDSRELHSRVEPVMALTEQQWELACWLARETASSVY
                     TAASLYLPRGIANRVVEWYRLAPDHEDRDDGELSNIQRGVVDVLRAESPLKLDTLRAR
                     TGRSLTKILPALEERRWVERVLQVDQRRAELPTTRYIRLLEPTAELPASASRQREVLE
                     SLRQRARMRRSGESDLIEESSLRQRLGVTRGIITALRDKGIIEEIELSDDATPKPAPA
                     PAPRLTASQERSWTAFRAALEAGDSTSHLLFGVTGSGKTEIYLRAVGWALTQGKTAIV
                     LVPEIGLATQVVRRFVERFPGRVAVLHSALKDAERLEVWEGIAEGRCDVVVGPRSALF
                     APMDRLGVIIMDEEHDSAYKQDTEPRYHARSVAIYLARQMGAGLIMGSATPAVESAWH
                     ADQHDYRKLLLPDRVSPSVVDAASGTAGALALPKVEIVDLRLELRQGNTSLLSSVLQG
                     YVRESLERGEQAILLLNRRGQSTVVLCRSCGSRLICPFCEIPLVFHRDQSKLICHRCN
                     FHERPPKVCPSCEGPLDYFGAGTQRVEEEARRLFPGVSVLRWDQDSVRRQGGYEAMLR
                     RVERREADIVVGTQMVAKGFDLPGVTTIGVINADTMIHLPDFRGAERTFQLLTQVAGR
                     AGRRGPGGRVAIQTYTPRHYAVQAAAEHDYGTFYAAEIAFREQHRFPPFTRLVRYVTR
                     HKNERQAALDAEKMARAVAKHARHADVEIDILGPTPAFAAKVRGEYQWQFVIRSHELD
                     ALLDGIPTAPGWVVDIDPQSMM"
     gene            8447..9280
                     /locus_tag="VGR22_10600"
     CDS             8447..9280
                     /locus_tag="VGR22_10600"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HEV2129056.1"
                     /translation="MSSSVTTITGEKEIRLADVDPAATDGLTEQEADRQFQELQNELR
                     ELHDLMMAAETHGLLLILQGMDAAGKDVTIENVAGALNPQAFRVKSFDKPTEEERMHH
                     FLWRADVATPMVGEAVVFDRSYYEQAMPEELQGDVFGERRARRFEHIKAFERILADEG
                     IIVIKVFLHIGKVTQEQRLENRQNEIDQAWKLSPSDWKKHRQWDVLIEAYETVLNGTA
                     TPDIPWHVVPADHRWYHNPAVARIVVERLREHREQWEASRREIGEQNRKEAQEARAEG
                     V"
     gene            9401..9940
                     /gene="def"
                     /locus_tag="VGR22_10605"
     CDS             9401..9940
                     /gene="def"
                     /locus_tag="VGR22_10605"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV2129057.1"
                     /translation="MAVMEILIDGDSRLRQKATKVRQVDTSLRKIADDMWETMEEADG
                     VGLAGPQVGVMRRIITVHVPENMDEEGDPEYRYTLVNPEIVRGFGNATAVEGCLSIPG
                     WAGEVARQESVTVKAMDLDNKPVRIKAKGYLARVFQHEIDHLDGILFTDRMAPEAQLF
                     RVEDEEHELNEEAAMLSHS"
     gene            complement(10195..10887)
                     /gene="gmk"
                     /locus_tag="VGR22_10610"
     CDS             complement(10195..10887)
                     /gene="gmk"
                     /locus_tag="VGR22_10610"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEV2129058.1"
                     /translation="MMNARLSDMRTLEAELNARFDHGDRHIERLRDTTAPRVFIISGP
                     SGVGKDTVLEQLRLTYPDAVYVVTATSRPKRRGEIEGVHYHFIDREEFEQQVANGEFI
                     ENAVVYNNLYGVPRTPIVDGIAAGKDVIIKVDVKGAATLRQKISNTVSIFLAPESMRE
                     LLNRLRLRKTDDYDALKRRFTTASDELERVEEFDYVVFNESEALDAAVRQITNIIEAE
                     KARVGPPVVTVE"
     gene            11006..12874
                     /locus_tag="VGR22_10615"
     CDS             11006..12874
                     /locus_tag="VGR22_10615"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HEV2129059.1"
                     /translation="MYDVLTTAAMVDELREHLLDGRVQKLGMVDPLTIAAEVYANGRR
                     QALVASADASHPRLYRASSLPSFDTSIVTPFSLQIRKYVRGGFIIDITQPLLERVVEL
                     TIARRLPDAAARRGAGPVEDDDNDDVTDEEDIWGRPDVVRTRLVVEIMGRHSNLILVG
                     EDGLVMESAKRVTPDMSRVRPVLPKREYTPPPPPETADPRRVTGPGIELVLAEEKPSR
                     KLQQALVRGLRAMSPQMAREVAYNVTGNADSVVGEPGMLDSQEIAREVRRLFEPMMLG
                     GWQPAVYRRGHVVSGYSAVAMAHLAVDADEERVETMSEAVEGTLEGARQEAPRDHEQL
                     RRRLVGRIGDARERLASRLRSLRNQQKRAEEADRFRHAGEMIYAYIWMIEPGQTVLEI
                     EGEEPIALDPSLDANGNAQAYFERYRKAQRGLEQVPVRIEAADREDAYLRQLATQAEQ
                     SQGFNTIEALTREFEEYLEEHPSGRAPDQRGQPQKKRKPSGQGSRPDQFRTPGGHLIY
                     VGHSGRQNDQVTFSIGGPDDTWLHARGVAGSHVIIRWDTAAEEQDSATIETAAALAGW
                     YSAARASGSVDVDVAERRHVRKISGAGPGMVTYRNEHTIVVAPQGESALREAGRIV"
     gene            complement(13227..14126)
                     /gene="tatC"
                     /locus_tag="VGR22_10620"
     CDS             complement(13227..14126)
                     /gene="tatC"
                     /locus_tag="VGR22_10620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEV2129060.1"
                     /translation="MARMIKVPAWKLQIPRLPKYDPNEPDIFEEMTLQEHLTELANRI
                     KKMVYGIVLGFIVGAILVNPILDQIREAAQVEQGLDIRSPSDPVIIFFRVALYVAVGI
                     TLPNIIYQLVAFLAPGLTGKEKRVLFSSLPFMSLLFVGGVAYAYFFAIPRALDFLSTF
                     LGDQLAWEIDAQETLSFYLALMMGLGIAFQLPVIMFVLAKIGIVTPANMRKWRKFAFL
                     GIVVAAAIITPTTDPINLGLVVMPLMILYEAGIIVSMVFAKTSLRSAADESNEVAELA
                     RPIPAKARTTSKAKMQSETPDDV"
     gene            complement(14347..14586)
                     /locus_tag="VGR22_10625"
     CDS             complement(14347..14586)
                     /locus_tag="VGR22_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV2129061.1"
                     /translation="MATVLERVQGIVAERLGVEESQVTEDAAFIDDLNADSLDLVEVI
                     MAMEQEFDLEISDEEAENIRTVGDAVSYIKEHQSA"
     gene            complement(14786..15376)
                     /gene="nusB"
                     /locus_tag="VGR22_10630"
     CDS             complement(14786..15376)
                     /gene="nusB"
                     /locus_tag="VGR22_10630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEV2129062.1"
                     /translation="MTDQHHDDTPAQPETDAVPEEQAASPADEAGKPRKRRNRPRNRK
                     RLMADRGPVTGTSTRHQARTLALQSLYEYDLTDHEGDDLRERLNADEDVPPSVREYAS
                     TLFGGVLEHLGPIDAQIGEAAPAFPVSQLAAVDRNVLRIAIYELMHQRKTVPVRVAIN
                     EAIEIAKHYGSERSGKFVHGVLGTVSRKLPDGEAKP"
     gene            complement(15430..15837)
                     /locus_tag="VGR22_10635"
     CDS             complement(15430..15837)
                     /locus_tag="VGR22_10635"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV2129063.1"
                     /translation="MSEQPATDAATTDVVTEGRSGSGTVRGTIRVAPEVLIELIELTV
                     RDIDGVAELRSRPRRQKAISETAGKCYDDGKVRVVVLGDQIETDIAVAMRAGTNVAEL
                     TAAIQKQVARAVERMLGMTASAVNIYIDDVVRA"
     gene            complement(15834..17258)
                     /gene="accC"
                     /locus_tag="VGR22_10640"
     CDS             complement(15834..17258)
                     /gene="accC"
                     /locus_tag="VGR22_10640"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEV2129064.1"
                     /translation="MFQKILIANRGEIALRVLRACRDLGVPAVVAYSEVDRDSLPVRL
                     ADEAVCIGPAPAPRSYNNIPAVISAALVTGCDAIHPGYGFLAENAYLADICRQVGLTF
                     IGPQAEVIERMGNKAEARRVMKKAGIPLLPGTEGVIRNHAEGRKIAKDIGFPVVIKAV
                     AGGGGRGMRVVRSEAEVARGISMAQSEAEAAFGNGDVYIERFLERPRHVEVQVIADHH
                     GKVLAVGERDCSLQRRHQKVVEEAPAPNLPKKVRDNLLKAATRGADAAGYTSAGTLEF
                     LLDQQGRFYFMEMNTRIQVEHPITEETAGIDLVAWQIRVAAGEKLPFRESDFEPRGHS
                     IECRITAEDAMNDFQPSVGTVRTYVAPGGPGVRVDSHLFGGYTIPPNYDSLLGKIITW
                     GKDRDEAINRMQRALHETVITGVQHTVPFHRAIIGDPEFRAGDVHTGFIPAFMERSFE
                     TLQTHAREEREADRHESMVKGTAR"
     gene            complement(17417..18157)
                     /gene="fabG"
                     /locus_tag="VGR22_10645"
     CDS             complement(17417..18157)
                     /gene="fabG"
                     /locus_tag="VGR22_10645"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEV2129065.1"
                     /translation="MANKGAIVTGGTRGIGLAIAKRLATDGYDLLITYRSDAEAAKSA
                     EAELSRTSRNVKVLAGDIANADDVGRIVEEGLGHLGQIDVLVNNAGITRDTLMMRMSE
                     QDWDDVISTNLKGAFLVSKAAMRPMLRARTGRIINLTSVVGLVGNTGQANYAAAKAGL
                     VGLTKSMAREVGSRGITVNAVAPGFIETRLTEVLPDKIKTQLLEQTPLGRFGTVDDVA
                     GAVAFLASDDASFITGQVLTVDGGLFMA"
     gene            complement(18296..19309)
                     /locus_tag="VGR22_10650"
     CDS             complement(18296..19309)
                     /locus_tag="VGR22_10650"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEV2129066.1"
                     /translation="MPFAAITGWGASVPERVLTNHDLEKMVDTSDEWIVSRTGIRERR
                     VVGEQDTTTSLAAAAGLQALERAGLAAEDLDLIVVGTATPDDFLVAQAAMVQAEIGGC
                     AGAFDVGAACAGFVTALSVGTQFIRCGTYERVLVVGVDTLTRYIDYTDRSTCVLFGDG
                     AGAVVLEPSDEERGLLSTVLGADGAGHKHLYVPGMASLVPESADLFPEYRPYLQMNGK
                     EVFRFAVQVMGEASAEAIERAGLGYDDVDMLIPHQANLRIIDAAARRLSLPRDKVWVN
                     VDRYGNTSAASVPIALAEAVDAGKIAEGDNVVLVAFGAGLAWAAGLVRWGTGGVARLS
                     GST"
     gene            complement(19531..19722)
                     /gene="rpmF"
                     /locus_tag="VGR22_10655"
     CDS             complement(19531..19722)
                     /gene="rpmF"
                     /locus_tag="VGR22_10655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEV2129067.1"
                     /translation="MGALPKQKISRHRQGNRRRHHHLAMPQLVRCRNCRSLKRAHHVC
                     PNCGTYRGRQVIEIEETRS"
     gene            complement(19797..20399)
                     /locus_tag="VGR22_10660"
     CDS             complement(19797..20399)
                     /locus_tag="VGR22_10660"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEV2129068.1"
                     /translation="MATGDHTARELVNETRINVASLLQEPVGSIRSIHLGMDAFLLDD
                     DLFANDLDADVRLIRLRHQVLADGKVRANVALDCVRCLTTFEQRVETTFSEPFLQSHD
                     VRSGADLSDHRERRDDEDADESDEESFVIDEGHEMDLAEALRQNLVLALPMVPSCGDV
                     CPGPPEEHLADKADERGGQFDLLSSLLDDDTDDEESAGSR"
BASE COUNT         3439 a         6441 c         7126 g         3614 t
ORIGIN      
        1 ccgcatgcat tgtgtgtggc aggagatcct tcggcatgga ctgcggcacc tgcggttcct
       61 tgccggctac ggatgacgtt atttcgtttg tcgtggtgaa ctgcatggcg atcggctcgg
      121 tcgcagggcg gggcctgtgg caagccgggg ccagttggca cggggccagc gccgtgggtt
      181 catcgcttgg gcgatagcac ccatttgttc agccacgtga cgatgcgctc catcaggtcg
      241 atctggtgct cgcgctcgcg gatagagtgc tcctcgcgcg ggtaccggac gaattgcacc
      301 ggcaccccgg cgtgctgcag cgctcggaag aactcctgcc cctgcgtcgg gtggacgcgg
      361 tcgtcgctgt cgccgtggag aatcagagtc ggggtggcaa cctgcgacgc ctcccggatc
      421 ggcgttccct tcgcgtagaa ctcccacgtc ccttcatcgt agggatggct ggggtaatag
      481 agcgtattgg cgtgcggaat gtccccggcg ccatgatccg atgcgaggtt gccgatgccg
      541 gcgcccatca cagcggctct gaaacggcgc gtctgggtga tggtgcgggc ggtgagatac
      601 ccaccccaac tccagccggc gatcgcgagc cgatcccggt ccgcgatgcc tcgctcgacc
      661 atcgcctccg ctccggcaat aagatccttc gactcgccac caccaacgtc gtcctgcagc
      721 agtttctcga acgccgagcc atacccggta cttccacgcg ggttcggcat cagcaccgcc
      781 catccccggg tggcgagcat ctgcgcccag tcgtgccagt cgagcatgac ccggtcttcc
      841 cactgccagg acggtccacc gtggacctcg acgaagaggg gcaatggtcc ctccgcgcgt
      901 gctggtcgca ccagcacgcc ctcgatctcg acaccgtcaa agctctgcca gcgaaccacc
      961 tcgccgggcg ccagcccgcc ctcgaatggt ttgccgaacg atgtgacctt ctctgtcgag
     1021 ccgttgcggt cgatggtcca gacttcctcc ggctcatcgc tcgctgtcca gacgaaggcg
     1081 acatggttgc cgtcccgtga cgcggagata ccagccgaga tcgacccatg ttcgcgcatc
     1141 gtgcccggcg agagcagctc gcgacggtcg gtctcgagtg acacgctgta gagccggccg
     1201 tgcacgccct cgacgatcac cgcgaggacg cgatctggtg tggtcgggtc ggcaacgatg
     1261 tcgtagaccg cgccgcgcag ctcagggagg atgttgcgcc gctcgccacc gtcccacggc
     1321 gcgatccaga tcgacggcga gggatcggcg cggtggtcgc tgtcgatcac cgcgactacc
     1381 tgctcgccac ggacgtcacg gacaaccggc gtgctgggag ccgtggggaa ggtcgtgatt
     1441 tcgtgttcca ggccaccggc gacgggtacg cgggcgaccc ggctcggcat gaaaaggctg
     1501 ttcgcggtcg gcagctccgt cgtgatcacg accagttcct gaccgtcggg tgcccaggcg
     1561 tagtcgcgaa cgcttcggtt gccggtggtc aggcatcggc gcttgccagt gtcggggtcg
     1621 atcacccaga ggcgctggtg cttcggctcc tcctccacga caacctgatc gcgtttcgcc
     1681 tccttgtcct tcttctcggc ctccgtctcg ggatccctca tgaggaacgc gatgcaggag
     1741 ccgtcgggtg accatcgcgg cttggcgatc tccccgcgca catcgccgat ctgctgggcc
     1801 tcaccgccgt cggccgggat gacgaagagg ccagcccgct catccttgtc atcacgggac
     1861 gacgtgaaga cgaggcagga gccatccggc gaccagccgg gatcggcagc gccggccttg
     1921 cctcccgtga aggggaaggg ttcgccgtga ttgcgtgaca gccagatctg cgaggttgcg
     1981 cggtcatcct ttttcgacgc atacatgacc gtcatcgcca ctgccgtgcc atctggagag
     2041 atgcgaggct cgcgcgggta ctggcggtcg gcgaggcggt cgaacgagat cggttcaggc
     2101 gtgggcatgc ggagctccat catcgcgagt tcggtcgtca ccctgcacgt ccgtatgtaa
     2161 gcgggcgtag cgtgcatggt cgatacgtgc tcccgagatt atcaggtagc tgcgtgcgat
     2221 gccctcgaac gcgaacccgc acttctcgag cacccggcgg gaggcgatgt tctccacggc
     2281 tgcgaccgct tccagccgct ccagagcgag accgctcgga tcgagggcag ttgccgccac
     2341 gtcgcgcacg gcacgggtcg caatgcctcg tgcctgaaag cgtggggcga tcgagtagcc
     2401 gatcgtgccg atgtggtgac tccgggaggt gacggacacc gtgacccagc cagcgacgtc
     2461 gccattgacg accaccgtcc attccagctt tccgttttgc cacggcgtca cctccgcatg
     2521 acctcgctcg tcgagaatca gtctcaggtc ctcgaccgaa cgctgttcga gcggctggta
     2581 gcgcctcgtc acgggatgcg cgcggatgcg gtggatcgct ggcgcatcgc tcgcttgtga
     2641 gcgtcgcagc acgtgatcgt cactcattcc tgctttcctg ttgcgcgacc gtccaggcgg
     2701 cgaggtcgtt ccatgtcatc gcgcggtgcc cgggcacctg ttcgccgcag gcgtgggtgg
     2761 atgtatcgac ggaaccgctc gtgtctgccc gagttgtgtg ctgatgcggt caaaataggt
     2821 gcgagtgtgg cactgtatag agagcacgta cgcgtgttgg ctcggttacg aatcaaggat
     2881 acgcaatggc gaaagatcgt ggcgcggcgg cccgacccgg tgaccgtcga ctctccgacg
     2941 atgtctatct gctcgcaggg ctgcttggcg aggtcattac ctcgatggcc ggggaggatg
     3001 ccttcgaact ggaggaggcg gcgcgcgcgc tggccaagcg gttgcggcgc ggcgacgaat
     3061 cggcaggcca gcagctggag atgctgatca gcgagtcgaa caccgacgac ctgcgcattc
     3121 tcattcgggc gttcaccaac tacttccagc tcatcaacct cgctgaggac aacgagcgta
     3181 tccgtcgtgt gcgatcccgg gagctcgcca gtcccaatga gccgcgtcgc ggctcgattc
     3241 ggcaggcgat tcagatgatg gtcgacggcg gggtggacgc cgagcagatg cagcatctgc
     3301 tcgaccatgc gcaggtcagg ctggtgctta ccgcacatcc gacggaggcg cggcggcgca
     3361 cggtgatcgc caagctcgcc cgcatcttcg cgatcatccg ggagctcgac gagcgtcacg
     3421 ccctcccgca cgaggtcacc cgggcgcgag ggctgctcgc gtcgaccatc gctgaactgt
     3481 ggagctccaa cgagctgcgg agcgtctcgc cgaccgtgct cgacgaggtg cggggtgtgc
     3541 tcgtctactt cgggtcgacc ctggtggagg tcatcccgct gatctaccgc gatctcgagg
     3601 aggcgctgaa ggacgcctat cccgatgccg agatcaccgt gccgccgttc atcacctttg
     3661 gttcgtggat cggtggcgat cgcgacggca atccgttcgt gaccccggat atcaccgagg
     3721 cgaccctgca tatcatgcgt gccgcggcgt tggcactgct cgaccatcgc ctcaccgagc
     3781 tcgccgggcg gctctcgctc tcggatgcga tggtgcggcc gagcgatcac ctccagccgc
     3841 tcatgacccg ttacgcgcgc cgcttcccgg agctggccgc gcagctccgg cagcaaaacg
     3901 cggacgagcc atatcgccag cttgtcatcc tgatgcggga gcggctgcgg gcgactcgtg
     3961 acggcggtga gcatggctat gctcgtggca gcgagctgct ggacgacctg aagcacgtgg
     4021 ctgcggcact gcgcgagcag tctgccggga cgatcctggg tggcgagctg cacgacgtca
     4081 tacggctggt cgaggtgttc gggttcgagt tcgtcacgct cgacattcgc gagcacagca
     4141 agcgtcatgg gcaggcattc gacgagatct ttcgggcggt tcgggtcgag gaggcctacc
     4201 tcgcgctccc cgaggaggag aagcaggcgc tgctggtgcg ggagatcaac gatccccgac
     4261 cactggtacc ggtcaacctc gaggtgctgc ccgagacggc gcggcaggtg atcgagactt
     4321 tccgcatgat ctggcgggtc cggcaccacg gatacccgga cgccatcgag acctacatca
     4381 tctccggcac cgacgcgccg tcggacatgc tggaggtgct gctgttcatg aaggagacgc
     4441 gcttggcgtc cgatggcggg caccatgcgt cgctgcggat cgcaccgctc ttcgaggagg
     4501 gcgcgacgct ggcggcatcc cgcgagacga tggcgaccgt gctcggctac ccggagtacc
     4561 gcgccgcgct ggaaagcgct ggcggtgtgc aggagatcat gatcggctac tccgactcca
     4621 acaaggatgt tggctacctg gcctccagtt ggcggctgca ccaggcgcag cgacgactgg
     4681 cccacttcct cgaggagcag gggatcgagt acctcttttt ccatggtcgc ggcggatcga
     4741 tcggacgcgg cggtgggccg accaacgttg cgatcatggc gttgccgagc ggcaccctga
     4801 acggtcgcat caagatcacc gagcagggcg aggtcatctc tgcacggtac agtatggcgc
     4861 agatcgccca ccgcgagctg gagctgaccg tcggggcgat cctcgcgcat gcggtgggcg
     4921 gcgaccggat taccgatccc gggcggcgcg agcgctacga gtcggtgatg gagcgcatgg
     4981 cagaggtctc cgccgagttc taccgcgatc tcgtctacgg cgaccccgac ttcggcacat
     5041 ttttccacca ggcgacgccg atcgacgcga tcgcgcggct ccagctcgga tcgcgccctg
     5101 cgaagcgcgt gcagtcggac cggatcgagg acctgcgcgc cataccgtgg gtcttctcgt
     5161 ggacacagtc gcgcatcatc ctcccggcat ggtacggcct cggcacggcg ttggaggatg
     5221 cgatcgagcg gcacggactc gacctgctgc aggagatggc acgcggctgg tcattcttca
     5281 cggcgttgct ctccaatgcc gagatggggc tcgccaaggc cgacctgact gttgggcacc
     5341 gctacgtcga gctggtcgag gatgcggtgg tccgcgaccg catctggggg cgaatcagcg
     5401 acgagtttgc gcggtcggta caacatctca ccgctgtgcg cggagaggag ctactgctcg
     5461 accatgactc tgtgctccag cgcagtgtcg agcggcgcaa tccctacgtc gatccgctct
     5521 cctacatcca agtggagctg ctggagcggc tgcgggagca acccatcgat gaggatgtcc
     5581 tgcagacgtt gcatctcgcc gtcaacggca tcgccggggg cctgaagaac accggatgat
     5641 cgcaacagcc actaccctcc atgccgatgt cgccgtcgat ggcgccggtc acgcaacgaa
     5701 cggcggcgtg ctgacgtaca ccgtgcccga gtgcctggca ggtttgctcg agcctcgcca
     5761 gctcgtctgg gtgcccctgc gttcgaagct ggtgctcggc atcgtctcgc gtgtccatga
     5821 cgaatcgccg cggttcgact cccgcgagct gcattcccgg gtggaaccgg tgatggcgct
     5881 cacggagcag cagtgggagc tggcctgctg gctggcgcgg gagacggcga gcagtgtcta
     5941 tacagcagcc tcgctctacc tgccgcgagg catcgccaac cgcgtggtgg agtggtatcg
     6001 gctcgcgccc gatcacgaag atcgcgacga cggcgagctc tcgaacatcc agcgcggcgt
     6061 ggtcgacgtg ctgcgggccg agagtccact caagctggac acgctgcgcg cacggaccgg
     6121 gcggtcgctg acgaagatcc tgccggctct cgaggagagg cgttgggtcg agcgcgtgct
     6181 gcaggtcgat cagcgtcgtg ccgagttacc caccacccgg tacattcggc tgctggagcc
     6241 gaccgccgag ttgcctgctt ccgcgagtcg ccagcgcgag gtactcgaat cgcttcggca
     6301 gcgcgctcgg atgcgccgat ccggagagtc cgacctgatc gaggagagca gcctccgcca
     6361 gcgactgggt gtgacgcgcg ggatcatcac ggcgctgcgc gacaagggca tcatcgagga
     6421 gatcgagctc tccgacgacg ccaccccgaa gccggcaccg gcccctgcac cgcgcctgac
     6481 cgcctctcag gagcggtcgt ggacggcatt ccgggcggcg ctggaggcag gcgattcgac
     6541 gtcgcacctg ctctttggcg tgacgggcag cggcaagacg gagatctacc ttcgcgccgt
     6601 cggctgggca ctgacgcagg gcaagaccgc gatcgtgctg gtgccggaga ttggattggc
     6661 gacacaggtc gtgcgccgct tcgtcgaacg cttccccggt cgggtggccg tgctgcactc
     6721 cgcactgaag gacgccgagc gactcgaggt ctgggaagga atcgccgagg ggcgctgcga
     6781 cgtcgtggtc gggccacggt cggcgctctt cgcgccgatg gaccggctcg gcgtgatcat
     6841 tatggacgag gagcacgaca gcgcctacaa gcaagacacg gagccgcgct accacgcgcg
     6901 cagtgtcgcg atctacctcg cccggcagat gggtgccggg ctcatcatgg ggagcgcgac
     6961 gccggccgtg gagtcggcct ggcatgccga ccagcatgat taccgcaagc tgctgctgcc
     7021 cgatcgggtg agcccgagcg tggtcgatgc cgccagcggc accgcaggtg cgcttgcact
     7081 gccgaaggtg gagattgtcg atttgcggct ggagctgcgg cagggcaaca cctcgttgct
     7141 cagctccgtg ttgcaggggt atgtgcgcga gagcctggaa cggggcgagc aggcgattct
     7201 gctcctgaac cgccgcgggc aatcgacggt cgtgctgtgc cgctcctgcg ggagccggct
     7261 gatctgcccg ttctgcgaga ttccgctggt ctttcatcgt gaccagtcga agctgatctg
     7321 ccatcgctgc aacttccatg agcgcccacc gaaggtctgt ccgagctgtg agggcccgct
     7381 cgattacttc ggcgccggca cgcagcgcgt cgaggaggag gcgcggcggc tcttccccgg
     7441 cgtctccgtg ctccgatggg atcaggacag cgtccggcgg cagggggggt acgaggcgat
     7501 gctgcgccgg gtcgagcggc gggaggcgga catcgtggtc gggacgcaga tggtcgccaa
     7561 aggatttgac ctgcccggtg tgacaacgat cggcgtgatc aacgccgaca cgatgatcca
     7621 tctgcccgat ttccgcggag cggagcggac cttccagctg ctgacgcagg tggcggggcg
     7681 tgccggacga cgtgggccgg gcggtcgggt tgcaatccag acgtacacgc ctcgccacta
     7741 cgccgtccag gcagccgccg agcacgacta cggcaccttc tacgccgccg agatcgcgtt
     7801 ccgtgagcag caccggtttc cgccgttcac gcggctggtg cgctacgtca cccggcacaa
     7861 gaacgagcgt caggcggcgc tggacgcgga gaagatggcg cgtgcggtcg caaagcatgc
     7921 ccggcatgcc gatgtggaga tcgacatcct cggcccgacg ccggcgttcg cggccaaggt
     7981 gcgcggcgag taccagtggc agttcgtcat ccgctcgcac gagcttgatg cgctgctcga
     8041 cggtattccc actgcacccg gctgggtggt ggacatcgac ccacagagca tgatgtagtt
     8101 cggcgaaggc gtaacgatgt catttcgacc gaagccccca cagggtgcgg agtgcaaaaa
     8161 ttccttgcct gccaaccatc gaccctcggt atgcaagaga tttctcgatt acacggattc
     8221 gccgctccgc tcgaaatgac tgcgttggct ggattgtcga ctgcaggcgc gcttcgctcg
     8281 ccgttccctg aagagggccg cggggtggca ttgcctggca tcctgcttgc aacagcaaca
     8341 ggctgaaccg ggcacgtagc ggcgggccct gcgcctgtgg gtcccgaccg tgctcacgtc
     8401 ttccgaacag tcggcgcatg caccgccgcc aggcacagga acattcatga gctcttcagt
     8461 gacaactatc accggcgaga aggagatcag gctcgccgac gtcgacccgg cggccactga
     8521 tggcttgaca gagcaggagg ccgacaggca gtttcaggag ctccagaatg agcttcggga
     8581 gctgcatgat ctcatgatgg ctgccgagac gcatgggttg ctgctgatcc tgcaggggat
     8641 ggatgccgcc ggcaaggatg tcaccatcga gaacgtcgcg ggcgcgttga acccgcaggc
     8701 gtttcgggtc aagtcgttcg acaagccgac ggaggaggag cgaatgcacc acttcctgtg
     8761 gcgggcggat gtcgccacgc cgatggtcgg cgaggccgtc gtcttcgacc gatcctacta
     8821 tgagcaggcg atgccggagg agttgcaggg tgatgttttc ggcgagcgca gggcgcggcg
     8881 gttcgagcac atcaaggcgt ttgagcgcat cctggccgac gagggcatta ttgtcatcaa
     8941 ggtcttcctg cacatcggca aggtgacgca ggagcagagg ctcgagaatc gtcagaacga
     9001 aatcgatcag gcctggaagc tctcgcccag cgattggaaa aagcatcgac agtgggatgt
     9061 cctcatcgag gcgtatgaga cggtcctcaa cggcaccgca acgccggaca tcccttggca
     9121 cgttgtgccg gctgatcacc gctggtacca caaccccgcc gtcgcccgaa ttgtcgtcga
     9181 gcggcttcgt gagcacaggg agcagtggga agcatcgcga cgcgagatcg gcgagcagaa
     9241 ccggaaagag gcacaggagg cccgtgccga gggagtgtag ggggctcgcg tgctccggga
     9301 agtgtcctgt atccagcgag agtcgaccca gagggtatga tagggggaaa gagattcgtg
     9361 agccgtatgc ggcggtttca cgctggagag tgcgtgcgac atggcagtta tggagattct
     9421 gatcgacggt gactcgcgcc tccgccaaaa ggcgacgaag gtgcggcagg tggatacgag
     9481 cctgcggaag atcgccgacg acatgtggga gacgatggag gaggctgacg gtgttggcct
     9541 cgccggccca caggtcgggg tgatgcgacg gatcatcacc gtccatgttc ccgagaacat
     9601 ggacgaggag ggcgaccccg agtaccggta cacgctggtc aacccggaga tcgtgcgagg
     9661 ctttggcaat gccaccgccg tcgagggctg cctgagcatc ccgggctggg cgggtgaggt
     9721 ggcccgtcag gagtcggtga cggtcaaggc gatggacctc gacaacaagc cggttcgcat
     9781 caaggcgaag gggtacctcg cgcgggtgtt tcagcacgag atcgaccacc tggacggcat
     9841 cctgtttacc gatcggatgg cgccggaggc gcagctcttc cgcgtcgaag acgaggagca
     9901 tgagctgaac gaagaggcgg cgatgctgtc gcactcgtag gctggtgtgg cgcgcgatgg
     9961 atttggagcc agcaacactg ctgtcatcct gagctgaagg cgcgtagcgc cgccgtgtcg
    10021 aaggattccc cgccattgca tttggggcag tggtggtctc ttgccagact gcgtgccggg
    10081 tatcgccaaa aggtcaggac gcgacatcaa gacaagagtc agtcggcgct tgggcgccga
    10141 ctgggggatc cctcgctccg ctcaggatga cagtcctctt ggaatgtccg atttctactc
    10201 caccgtcaca accggcgggc cgacacgggc cttttcggcc tcgataatgt tcgtgatctg
    10261 acggacggcc gcgtccagcg cctccgactc gttgaagacc acgtagtcga actcttccac
    10321 ccgctccagt tcgtcactcg ccgtggtgaa ccgtcgcttg agggcgtcgt agtcgtccgt
    10381 ctttcgcaag cgcaggcggt tcagcagctc gcgcatcgac tcgggcgcga gaaagatcga
    10441 gacggtgttg ctgatcttct ggcggagggt ggctgcaccc ttgacgtcca ccttgatgat
    10501 gacgtccttg ccggcggcga tgccgtcgac gatcggcgtg cgcggaaccc cgtagaggtt
    10561 gttgtagacg acggcgttct cgatgaattc gccgttggca acctgctgct cgaactcctc
    10621 acgatcgatg aagtggtagt ggacgccctc aatctcaccg cgacgcttgg ggcgagaggt
    10681 ggcggtgacc acgtagacgg cgtcggggta ggtgagccgc agctgctcga gcacggtgtc
    10741 cttgcccacg ccggatggtc ccgagatgat gaacacgcgc ggagcggtgg tgtcgcggag
    10801 cctctcgatg tggcgatcgc cgtggtcgaa ccgggcgttc aactcggcct ccagggttcg
    10861 catatcgctc agtcgcgcgt tcatcatcga ttggtcgatc cttccgggac aacatcgccg
    10921 attggcgatt gcggggtcga gttgtgtcac gatagtacga tgaattgccg ctttggtatg
    10981 gaacacatga ggactagcct gcaacatgta tgacgtgctc accaccgccg ccatggtcga
    11041 tgaactgcgc gagcatctgc tcgacgggcg ggtgcagaag ctcggcatgg ttgacccgct
    11101 caccatcgcc gctgaggtct acgcgaacgg ccgtcggcaa gcgctggtcg ccagcgctga
    11161 tgcatcgcat ccccgcctct atcgtgcctc gtcactgccc tcgttcgata cctcgatcgt
    11221 tacgccgttc agcctccaga tccgcaagta cgtccgggga ggcttcatca tcgatatcac
    11281 ccagccgctc ctggaacgcg tggtcgagct gaccattgcc cggcgattgc cggacgcggc
    11341 ggcgaggcgc ggcgccggtc cggtcgagga cgatgacaac gatgacgtga ccgacgaaga
    11401 ggacatctgg ggtcgtccgg acgtcgtgcg aaccaggctt gtggtcgaga tcatggggcg
    11461 gcacagcaac ctgatcctcg tcggcgagga cgggctcgtg atggagagcg cgaagcgcgt
    11521 gacgcccgac atgagtcgcg tgcggccagt gctgccgaag cgggaataca cgccgcctcc
    11581 tcctccagag acggccgatc ctcgtcgtgt gaccggaccc ggcatcgagc tggtgctggc
    11641 ggaggagaag ccgagccgca agctgcagca ggcgctggtg cgcggactgc gcgccatgag
    11701 cccgcagatg gcacgggagg ttgcctacaa cgtgaccggg aacgctgaca gcgtcgtggg
    11761 tgagccgggc atgctcgatt cgcaggagat cgcgcgggag gtgcggcggt tgttcgagcc
    11821 gatgatgctc ggcggctggc aaccggcggt ctaccggcgc ggccacgtcg tttccggcta
    11881 cagcgcggtg gcgatggccc accttgccgt ggacgcggat gaggagcggg tcgagacgat
    11941 gtccgaggca gtcgagggga ccctggaggg tgcccggcag gaggcgccgc gggaccacga
    12001 gcagcttcgg cggcggctgg tcgggcgaat cggcgacgcg cgggagcggc tcgcgagccg
    12061 actccgctca ctccgcaacc agcagaagcg cgccgaagag gcggatcggt tccggcacgc
    12121 cggcgagatg atctatgcgt acatatggat gatcgagcca gggcagacag tgttggagat
    12181 cgagggtgag gagccgatcg cgctcgatcc ctcgctggat gccaacggca acgcgcaggc
    12241 gtacttcgag cgttaccgaa aggcacagcg cgggctggag caggtgccgg tgcggatcga
    12301 ggcggccgat cgggaggatg cctacctgcg gcaactcgcc acgcaggcgg agcagtcgca
    12361 ggggttcaac acgatcgagg cgctgacgcg ggagttcgag gagtacctcg aggagcaccc
    12421 gagtggccgc gcaccggatc agcgcggtca gccgcagaag aagcgcaaac cgtccgggca
    12481 ggggagccgc cccgaccagt tccgtacacc gggcgggcat ctgatctatg tcggccactc
    12541 cgggcggcag aacgaccagg tgacgttctc gatcggcgga ccggacgaca cctggctgca
    12601 cgcgcggggc gtcgcgggct cgcacgtgat cattcggtgg gacacggcgg cggaggagca
    12661 ggactcggcg acgatcgaga cggcggcggc gctggctgga tggtacagcg ccgcccgcgc
    12721 gagcgggtcg gtcgacgtcg atgttgctga gcgacggcat gtgcgcaaga tctcgggcgc
    12781 ggggccgggc atggtgacgt atcgcaacga gcacaccatc gtggtcgcgc cgcagggcga
    12841 gagcgccctc cgcgaggcgg gccggatcgt ctgagcggcg aaggcgtgaa tcgcggatgg
    12901 tgccatagcc tgtgcccctg gctgaggtgc ccggatgtcg ccgtggttgt caatacaggg
    12961 ggtcagagaa agcagcggag cggtatcagc ctgagcggag gtgcgaagca ccggagtcga
    13021 aggatctcct gcgtgccgct ctcaggggct tggggggaga cacctgcggc gcaggggatc
    13081 cttcgacatg gcatcggcac cgtcggtgcc ttgttggctc aggatgacat cgtttcgttg
    13141 agcttcagtg agccgaaccg ggcaccaacg gaaaaccccg gccactggcg gaccggggct
    13201 ccctgtgtgc acgttgtctt tcgatgctac acgtcgtcgg gggtttccga ctgcatcttg
    13261 gccttcgagg tcgtcctggc cttcgccggg atcggtcgcg ccagctcggc aacctcgttt
    13321 gactcgtcgg cggcactgcg cagcgaggtc ttggcgaaaa ccatcgagac gatgatgccg
    13381 gcctcgtaga ggatcatcag cggcatcacc accagcccga ggttgatcgg gtcggtcgtc
    13441 ggggtgatga tcgccgccgc gaccacgatg ccgaggaagg cgaacttccg ccacttgcgc
    13501 atgttggccg gcgtgacgat gccgatcttg gccagcacga acatgatgac cgggagctgg
    13561 aaggcgatgc cgagtcccat catcagggcg agatagaagc tgagggtctc ctgcgcgtcg
    13621 atctcccagg caagctggtc acccaggaag gtcgagagga agtcgagggc gcgcgggatc
    13681 gcgaagaaat aggcgtaggc cacgccaccg acgaagagca gcgacatgaa cgggagcgag
    13741 ctgaagagca cccgtttttc cttgcccgtc aaaccgggcg cgaggaaggc caccaactgg
    13801 tagatgatgt tcggcagggt gatgccaacg gcgacataga gggcgacacg gaagaagatg
    13861 atgacggggt cggacggcga gcggatgtcg agtccttgct cgacctgagc cgcctcgcgg
    13921 atttggtcga gaatggggtt gacaagaata gcgccgacga tgaagcccag tacgataccg
    13981 tagaccatct tcttgatacg gttcgcgagc tccgtcagat gctcctggag cgtcatctcc
    14041 tcgaagatgt cgggctcgtt gggatcgtat ttcggaagcc gtggaatctg caacttccac
    14101 gccggaacct tgatcatgcg tgccatgcga tggttcgccc tttccaggcc tgagtcagtg
    14161 ggtacgccaa cgcgggaaag cggcgccttc gggcgcgcgg ctgggccatg taacgtcagc
    14221 gacggaagtg acgaccagaa tgggacacac ggttcgtcgg ggatgtccag gaagggtgga
    14281 cgagcaaggg agcacggtct tcactcgcgc tcccccgtgc tgccggaggt cgcacgcgtt
    14341 cgtgcgtcag gcgctctgat gctccttgat gtaggaaacc gcatcaccga cggtgcggat
    14401 gttctcggct tcctcatcgc tgatctcgag gtcgaactcc tgctccatgg ccatgatgac
    14461 ctcgaccagg tcgagcgaat ccgcgttgag atcgtcgatg aacgccgcat cctcggtcac
    14521 ctgcgactcc tcgacaccga ggcgctccgc gacgattccc tgaacacgtt cgagtacagt
    14581 agccaaccct gttcctccct gttgctggcg gttccaacga accggtcccc cgacatgtcc
    14641 ggttggacac tcgtacttcc gtcaatcata cccgttgcgg cacagcaaaa ccgccgcgca
    14701 cggcaagcct ctggccgctt ccagtgtacg cgaggattct acacgaccgg cgaggggcga
    14761 ggtaggcgcg agagacgctg aatgttcagg gcttcgcttc gccgtcgggc agcttgcgcg
    14821 agaccgtgcc gaggacgcca tgcacgaact tgccggaccg ctccgagccg tagtgcttgg
    14881 cgatctcgat cgcctcgttg atcgcgacgc gcaccggcac ggtcttgcgc tggtgcatca
    14941 gctcgtagat ggcgatgcgc agcacgttgc ggtcgaccgc ggccagctgg ctcactggga
    15001 aggccggcgc ggcctcgccg atttgtgcgt caatcggccc caggtgctcc agcacgccgc
    15061 cgaagagggt ggaggcgtac tcccgcacgg aggggggcac gtcctcgtcc gcattgagcc
    15121 gctcccgcag gtcatcgcct tcgtggtcgg tcaggtcgta ttcgtagagc gactgcaggg
    15181 ccagcgtgcg ggcctggtgg cgtgtcgagg tgcccgtcac cggccctcgg tcggccatga
    15241 ggcgcttgcg attgcgaggc cggttgcgcc gctttcgggg cttgccggcc tcgtcggcag
    15301 gtgaggctgc ctgctcctcc ggaaccgcat cggtctccgg ctgggcaggg gtgtcgtcgt
    15361 ggtgctgatc agtcatggag atgcgggatg tccttgtgcg ggtaatcgag taggcggcgc
    15421 ggcgccgtgt caggcgcgga cgacgtcgtc gatgtaaatg ttgaccgcgc ttgctgtcat
    15481 gccgagcatg cgctcgacgg cgcgagcgac ctgcttctgg atcgctgccg tcaactccgc
    15541 aacgttggtg ccggcgcgca tggcgacggc gatatcggtc tcgatctggt cgccgagcac
    15601 gaccacgcgc accttgccgt cgtcatagca tttgccggcc gtctcgctga tggccttctg
    15661 ccggcggggg cgggagcgca gctcggcgac cccgtcgata tcgcgcacgg tcaactcgat
    15721 caactcgatg aggacctccg gtgcaacgcg gatggtgccg cgcacggtgc cggagccgct
    15781 gcgtccttcg gtgacgacgt cggtcgtggc ggcgtcggtt gccggttgct cgctcatcgc
    15841 gcggttcctt tcaccatgct ctcgtgtcgg tcagcctcgc gctcctcgcg ggcatgggtc
    15901 tgcagcgttt cgaacgaccg ctccatgaac gccgggatga acccggtgtg gacatcaccc
    15961 gcccggaact cgggatcacc gatgatggcg cggtgaaagg gcacggtgtg ctggaccccg
    16021 gtgatcaccg tctcgtgcaa cgctcgttgc atgcggttga tcgcttcgtc gcggtccttg
    16081 ccccacgtga tgatcttgcc aagcagcgag tcgtagttgg gcgggatggt gtagccaccg
    16141 aacagatggg agtcgacccg cacgccgggc ccaccgggag cgacataggt gcgcacggtg
    16201 ccgaccgagg gctgaaagtc gttcatcgca tcctcggcgg tgatgcggca ctcgatggag
    16261 tggccgcgcg gctcgaagtc actctccctg aacgggagct tctctccggc ggcgacgcgg
    16321 atctgccacg cgacgaggtc gatgcccgcg gtttcctcgg tgatgggatg ctcgacctgg
    16381 atacgcgtgt tcatctccat gaaatagaag cggccctgct ggtcgagcag aaactccagc
    16441 gtgccggcac tggtgtagcc ggccgcatca gcaccgcgcg tggcggcctt gagcaggttg
    16501 tcgcgcactt ttttcggcag gttcggcgcg ggagcctcct cgacgacctt ctggtgtcgg
    16561 cgctggaggc tgcagtcgcg ctcgccgacg gccagaacct tgccgtggtg gtcggcgatc
    16621 acctggacct cgacgtggcg cgggcgctcc agaaagcgct cgatgtagac atcgccgttg
    16681 ccgaaggcgg cctccgcctc ggactgtgcc atggagatgc cccgcgcgac ttcggcctcc
    16741 gagcggacga ctcgcatgcc acgaccgccg ccgccggcga cggccttgat gacgaccggg
    16801 aagccgatgt ccttcgcgat cttgcggccc tcggcatggt tacggatcac cccctcggtg
    16861 cccggcagca gggggatgcc cgccttcttc atcacgcgtc gcgcctcggc tttgttgccc
    16921 atccgctcga tcacctcggc ttgtggaccg atgaaggtga ggccgacctg cctgcagatg
    16981 tcggcgaggt aggcgttctc ggcgaggaag ccgtagccgg ggtggatggc atcgcaaccg
    17041 gtgacgagcg cggcgctgat caccgccggg atgttgttgt aggagcgagg ggcgggggcc
    17101 gggccgatgc agaccgcctc gtcggcgagc cggaccggca gcgagtcgcg gtcgacctcg
    17161 ctgtaggcga ccaccgcggg gacgccgagg tcgcggcagg cccgcagcac gcgcagggcg
    17221 atctcgccgc ggttggcaat caggattttc tggaacatgc agcgctctcc tgccgagttg
    17281 ccggacgcaa cgcgcgttca agtgtaccgt gatggagacc gagacccgct catgcgaaat
    17341 ggccggaccc ggcgagaact agggttgacg tggagtcgac ccgtatggat ggcccgccat
    17401 accggccttg tcccggctac gccatgaaca ggccgccgtc gacggtgagc acctggccgg
    17461 tgatgaacga tgcgtcgtcc gaggcgagga aggcgaccgc accggcgacg tcgtccacgg
    17521 tcccgaagcg gccgagcgga gtctgctcca gcagctgggt cttgatcttg tccggcagca
    17581 cctcggtgag acgggtttcg ataaagcccg gcgcgaccgc gttcacggtg atgcctcgcg
    17641 agccgacctc cctggccatc gacttggtca ggccgacgag tcccgccttg gcggcggcgt
    17701 agttggcctg tccggtgttg ccgaccaggc cgacgaccga ggtgagattg atgatacggc
    17761 ccgtccgggc gcgcagcatc ggtcgcatgg ccgccttgct gacgagaaag gcgcctttga
    17821 ggttggtcga gatgacgtcg tcccagtcct gctcgctcat gcgcatcatc agcgtgtcgc
    17881 gggtgatgcc ggcgttgttg acgagcacgt cgatctggcc gaggtggccg agcccctcct
    17941 cgacgatgcg cccaacgtca tcggcattgg cgatgtcacc ggcaaggacc ttgacgttgc
    18001 ggctcgtccg ggagagctcc gcctcggcgg acttcgcggc ttctgcgtcg ctgcggtagg
    18061 tgatcaggag gtcgtagccg tcggtggcga gccgcttcgc gatagcgagg ccgatgcccc
    18121 gtgtcccgcc ggtaacgatc gcgcccttgt tcgccataag atgtcgtcct ttcctcacct
    18181 caggatcctg gatcccgtgg cagaagggtg gacatggagt ccgcccgtac gggttgactc
    18241 catgtccacc ccctcgtgtt ctgggtccga tggggcagcc cctgtggaca ccgtgtcagg
    18301 tcgatcccga gagccgcgcg acgccgcccg tgccccaacg cacgaggcca gccgcccacg
    18361 ccagcccagc gccgaacgcc accagcacaa cgttgtcccc ctcggcgatc ttgccggcgt
    18421 cgaccgcctc ggcgagtgcg atcggcacgg aggccgccga ggtgttgccg tagcggtcga
    18481 cgttgaccca caccttgtcc cgcggcagcg agagccgccg tgcagccgcg tcgatgatgc
    18541 gcaggttggc ctggtgcggg atgagcatgt cgacgtcgtc gtagccaagc ccggcgcgct
    18601 cgatcgcctc ggcggaggct tcgcccatga cctgcacggc gaagcggaag acctccttgc
    18661 cgttcatctg gagatacggt cggtactcgg ggaagagatc ggccgattcc ggcacgaggc
    18721 ttgccatgcc gggcacgtag agatgcttgt ggccggcccc gtccgcgccg agcacggtcg
    18781 agagcaggcc gcgctcctcg tcggacggtt ccagcaccac agcgccagcg ccatcgccga
    18841 agaggacgca ggtggagcgg tcggtgtagt cgatgtagcg ggtgagggta tcgacgccga
    18901 cgaccagtac gcgctcgtag gtgccgcagc ggatgaactg ggtgccgacg ctgagggcgg
    18961 tgacgaagcc cgcgcaggcc gccccgacgt cgaaggcgcc ggcgcagccg ccgatctcgg
    19021 cttggaccat ggcggcctgg gcgacgagga agtcgtccgg cgtggcggtg ccgaccacga
    19081 tcaggtcgag atcctccgcg gcgagtccgg cgcgctcgag cgcctgcagc ccggcggcgg
    19141 cggcgaggga ggtggttgtg tcctgctcgc cgacgacacg acgctcgcgg ataccggtgc
    19201 gcgacacgat ccactcgtct gaggtgtcga ccatcttctc gagatcgtga ttcgtgagca
    19261 cacgctcggg cacggaggcg ccccaaccgg ttatggccgc gaatggcatg gcgagtcctg
    19321 acgtgattcg ggtggcgacg aacgaatcgt ttcgctatcg ccgggaattg taaagcggaa
    19381 cggtgctcgt atgcccggcc tgtgcgggcc accgggctgc ggcatggcgg gcacgaaaaa
    19441 aggcggggaa cacagttcac ccgccttctc gtggttatgg tggagccggt cgggcggcgc
    19501 gatggtcgcc cagagtggtg cctgtggtgt ctaggaccgg gtttcctcga tctcgattac
    19561 ctgacgaccg cggtaggtgc cgcaattcgg gcagacgtgg tgcgcgcgct tgaggctgcg
    19621 gcagttgcga cagcgtacga gctgcggcat cgcgaggtgg tggtggcggc ggcggttgcc
    19681 ctgccggtga cggctgatct tctgcttcgg aagcgcgccc atggcggttc tccttttctg
    19741 tgtctgccga ggatcccgaa gggcgggctc cggcggcgtg ctggtgcggg ctatagctag
    19801 cggctgcccg cggactcctc atcgtcggtg tcgtcgtcga gcaggctgga cagcagatcg
    19861 aactgccccc cgcgctcgtc ggccttgtcg gccaggtgct cctccggcgg accggggcaa
    19921 acatccccgc agctcggaac catcggcagg gcgagcacga ggttctggcg gagggcctcc
    19981 gccaggtcca tctcgtggcc ctcgtcgatc acgaacgact cctcgtcgga ctcgtccgcg
    20041 tcctcgtcgt cgcggcgctc gcgatgatcg ctgagatccg cgccgctgcg gacgtcgtga
    20101 gactgcagga acggctcact gaacgtggtc tcgacccgtt gctcgaacgt ggtgagacag
    20161 cgaacgcaat ccagcgcgac attcgcgcgc accttgccgt cggcgagaac ctgatggcgc
    20221 aagcggatca gccgcacgtc agcgtcgaga tcgttcgcga agaggtcgtc gtcgagcagg
    20281 aatgcatcca tgcccaggtg aatcgatcga atgctgccca ccggttcctg gagcagcgac
    20341 gcgacgttga ttcgggtttc gttgacgagc tctctcgcgg tgtggtcgcc tgttgccatc
    20401 tgtatctaac cgacgcgcat tcgctgaccc gacccgctgg tgcggggcaa cgacgcgcac
    20461 gaagacgccg ggcgcaaatc ccggcgtagc cgtcaaagta tagggaacgg cgggtgcttc
    20521 gtcaatcagg gagagggata ggttataggc cccgattctg tgcaccctct aggctctccc
    20581 aagtgtgaac ctacatcgcc accgccctac gaaggtttcc
//
LOCUS       DASYWL010000302         3457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_671996, whole genome shotgun sequence.
ACCESSION   DASYWL010000302 DASYWL010000000
VERSION     DASYWL010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671996"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1737)
                     /gene="ggt"
                     /locus_tag="VGR22_10665"
     CDS             complement(91..1737)
                     /gene="ggt"
                     /locus_tag="VGR22_10665"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEV2129069.1"
                     /translation="MSRTREHVMTSELQSELAQQAGPPRDLTMSRRTMAASVNQLVSQ
                     TAIDVLRRGGNAVDAAIAGAAMLMVVEPRNGHLGGDTFMLFHDARQDCVIALNGSGAA
                     PARATLEHYREIGGIPEDGLLTCTVPGTVACWGHANERYGSVPLAELLAPAIAHADEG
                     IPVTTRLHHMLTLDAPTYRTSPDSAAVFLPDGRVPSVGAIWRQPGLAASLRRVAEHGV
                     DDFYRGELARELVDYSDRHSGLFTLDDLARHETQERPPLSMDYRGYTVHEQPPVSQGI
                     LVLLALNILAQFDLQQYGPGTAETLHLEIEALKLAFEDQAAYLGDPAFVDISVGHLLS
                     EEHAREQASHIDLYSARSGLAPAPSHPDTTYMCTADESGSMVSYIHSLYAGAGVVMGD
                     TGVLMNSRLQGFNLDEGHPDCLAPGKRPLHTLNNYVVHKDGKPILVGGTPGAHWQVQT
                     NLQILVNILEFGMDVQQAVEAPRFTMGDQLSLGSQTVRIESRVGQDAADALTDRGQQV
                     EVVGPWDAPGAVQLIMRDPETGIYRGATEVRRAGSTVLGF"
     gene            complement(1875..2345)
                     /locus_tag="VGR22_10670"
     CDS             complement(1875..2345)
                     /locus_tag="VGR22_10670"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEV2129070.1"
                     /translation="MPASGYSSHSQLTRTEVAHIVEQRVQQNHLTDQGDILWLATELK
                     ASGEMVGSVHLAWLSQEHAQGEVGYRLHPDHWGQGYASEAVRTMLAYGFRDLDLHRII
                     GTCDDHNEPSRRLLERIGMRQEAHFREIEWIKGEWRSQLVYAILRGEWESSVNP"
     gene            2722..3027
                     /locus_tag="VGR22_10675"
     CDS             2722..3027
                     /locus_tag="VGR22_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129071.1"
                     /translation="MRRVALRRILLVLLSTVLVLGTIGMPAAAQDAPDPDPTTVTTQS
                     DDESVTELPATGRGQGLSASDTERFVAEQMLVTVLVLITAATLSLGAIALAWRYDRQ"
     gene            complement(3098..>3457)
                     /locus_tag="VGR22_10680"
     CDS             complement(3098..>3457)
                     /locus_tag="VGR22_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129072.1"
                     /translation="DRYGAESLEATLVANRPNKRILQLDRTVNTGRPVMLTEYGGISY
                     APREGERWYGYGTVHSADAYLEKVRELTHAIRGCPGISGFCYTQLTDTEQETNGLLTE
                     HRESKLPVESLREIFGE"
BASE COUNT          556 a         1109 c         1137 g          655 t
ORIGIN      
        1 atcagggact gctggcgatc ggctctcacg ccggtgtcac aatcgtcacc gcgcgtcagt
       61 tcctggagat cttggacact gagtgaggtg ctagaagccc agcaccgtgc ttcccgcccg
      121 acgtacctct gtcgctcctc ggtagatgcc ggtctccgga tcgcgcatga tcagctggac
      181 cgccccggga gcgtcccacg gtcccacaac ctcgacttgc tggccccgat cggtgagcgc
      241 gtctgcggca tcctgaccga ctcgtgactc gatccgcact gtctggcttc cgagcgagag
      301 ctggtcgccc atcgtgaagc gcggcgcttc caccgcctgc tgcacgtcca tcccgaactc
      361 gaggatgttg acgaggatct ggaggttcgt ctgcacctgc cagtgcgcgc ccggcgtccc
      421 gccgacgagg atgggcttgc cgtccttgtg tacgacgtag ttgttgaggg tatggagcgg
      481 gcgcttccct ggcgccaggc agtccggatg tccctcgtcc aggttgaagc cctgtagtcg
      541 actgttcatc aggacaccag tatcccccat gaccacgcca gcgccggcat agaggctgtg
      601 gatgtacgaa accatcgatc cggactcgtc cgccgtgcac atgtaggtcg tatccgggtg
      661 cgaaggcgcg ggcgcgagcc cactcctcgc actgtacagg tcgatgtggc tcgcttgctc
      721 gcgcgcatgt tcctcggaca gcaggtgccc caccgagata tcgacgaacg ctgggtcgcc
      781 aagatacgcc gcctgatcct cgaaggcgag tttgagcgct tcgatctcga gatggagcgt
      841 ctcggcggtg ccggggccgt attgctggag atcgaactgc gcgaggatgt tcagcgcgag
      901 caggacgagg atgccctgcg agaccggcgg ctgctcgtgg acggtgtagc cgcgatagtc
      961 catcgagagc ggggggcgct cttgcgtctc atgtcgggcg aggtcgtcga gcgtgaacag
     1021 accgctatgc cgatcggagt agtcgaccag ctcccgggcc agttcgcccc ggtagaagtc
     1081 gtccacgccg tgctcggcga cgcgtcggag cgacgccgcg agccccggct ggcgccagat
     1141 agcgccgacg gacggcaccc ggccgtccgg caggaagacg gcagcgctgt cgggggacgt
     1201 gcggtaggtt ggcgcatcca gcgtcagcat gtggtgcagg cgtgtcgtga ccgggatgcc
     1261 ctcatccgcg tgcgcgatcg ctggcgcgag tagctcggcg aggggcactg agccgtagcg
     1321 ctcgttggcg tgaccccagc acgccacggt gccggggacc gtgcaggtga gtagtccatc
     1381 ctccgggatc ccgccgatct cgcgatagtg ctcaagtgtt gccctggctg gcgcagcgcc
     1441 actgccgttg agcgcgatca cgcagtcctg ccgcgcgtcg tggaacagca tgaaggtgtc
     1501 accgccgaga tgaccgttcc gcggttcaac gaccatgagc atcgccgctc cggcaatcgc
     1561 cgcgtcaacc gcgttcccgc cgcgccgcag cacgtcgata gccgtctggc tgacgagctg
     1621 gttgacggag gcagccatgg tgcgccggct catggtgagg tcgcgcggag gacctgcctg
     1681 ctgcgcgagc tcgctctgca gttcgctggt catcacgtgc tctcgagtac ggctcactgg
     1741 cgctgccttt cgctttggga tactgcacgg aattgggccg tcgcctgatc ttccctgcct
     1801 gatcgagatg ggtgggatgg cagggccgaa actccggccg atcggcatgg ggggtggaga
     1861 acagtcctac ggagttacgg attaacagac gattcccact cgccgcggag tatggcgtag
     1921 accaactggc tgcgccattc gcccttgatc cactcgatct cgcggaagtg agcctcctgg
     1981 cgcatgccga tacgctcgag caggcgtcgt gaaggctcgt tgtggtcgtc acaggttccg
     2041 atgatgcggt gcagatcgag atcgcggaag ccgtaggcga gcattgtccg caccgcctca
     2101 ctcgcgtacc cctgtcccca gtgatccgga tggagccggt agccgacttc cccttgggcg
     2161 tgctcctggc tcagccaggc gagatgcacg gagccgacca tctcgccgga cgccttcagc
     2221 tcggttgcca accacaagat gtcgccctga tcggtcaggt ggttctgctg aacgcgctgc
     2281 tcaacgatgt gggctacctc ggtgcgggtg agttggctgt gcgaactata ccccgatgcg
     2341 ggcatacata ttgctaccag acggcgatcg ccctcaccgg aggcagcccg cgaggaggtt
     2401 gccgccagtg taccggcggg tgacaatgtc gacactctcg atatcgatgg tatcgctggc
     2461 ggggataatg acgacgcatt gtggtcccgg atgatcgcat ttgtcgtcga gcccatgcag
     2521 cctgaggact ggagccggcg cgatatgcat cgtattgtgg tccatgcacg ccgcgcctac
     2581 ccgaaaccct gtagtgttgc tgcactgatc tgcatggaac cgaacagcgt ccccgggcgt
     2641 agagcatgac acagggacat ttcccccggg cgcggatgcg cttcggtgtg ggattgtcgg
     2701 atcaggtggt tggggagagc gatgcgacgt gtagcgctca gacgaatctt gctggtgctg
     2761 ctcagcacgg tgctggtgct gggcaccatc ggtatgcccg ccgccgcgca ggacgcgcca
     2821 gaccctgacc caaccaccgt cacgacgcag agcgatgacg agagcgtcac ggagttgccc
     2881 gccacggggc gcggtcaagg actctcggcg agcgacaccg agcgattcgt cgccgagcag
     2941 atgctggtca ccgtgctcgt gctgatcacc gctgccaccc tctcgctcgg ggcgattgcg
     3001 ctcgcctggc gctacgaccg gcagtgaccg tcagtcagcg aacgtggcca tctgctgcat
     3061 cagttgctgc ctgctcccct gtcagcggta tagggctcta ctccccgaag atctcgcgca
     3121 gtgactccac cggcagcttg gactcccgat gttcggtcag caagccgttg gtttcctgct
     3181 cggtatcggt cagttgcgta tagcagaatc cgctgatgcc ggggcagccg cggatcgcgt
     3241 gggtcaactc ccgcaccttc tccagatagg cgtcggccga gtgcaccgtg ccgtacccgt
     3301 accagcgctc gccctccctg ggcgcgtagc tgatgccccc gtactccgtc agcatcaccg
     3361 gacggcctgt gttcaccgtc cggtcgagtt gcaggatccg cttgttcggc cggtttgcga
     3421 ccagtgtggc ctccaagctc tccgcgccgt acctgtc
//
LOCUS       DASYWL010000303        11458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_484993, whole genome shotgun sequence.
ACCESSION   DASYWL010000303 DASYWL010000000
VERSION     DASYWL010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 11458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11458
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484993"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VGR22_10685"
     CDS             <1..340
                     /locus_tag="VGR22_10685"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEV2129073.1"
                     /translation="WEHHPVWFHGDVAVGNLLLGDGRLCAVIDFGTSGVGDPACDLAV
                     AWTLFTGESREAFRAALPFDEATWARGRAWALWKALILVAGIAETNAREAERSRQIID
                     EILADYRRVG"
     gene            complement(687..4178)
                     /gene="metH"
                     /locus_tag="VGR22_10690"
     CDS             complement(687..4178)
                     /gene="metH"
                     /locus_tag="VGR22_10690"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEV2129074.1"
                     /translation="MSRFLDLVNTRPVFYDGSMGATILNLGLTPEDYGGQATEGYNDY
                     LSVVKPEVIQQIHASFLEIGADVIETNTFGSSVIKAEEYGLGAQTYDINLASAKLARR
                     VADEYSTKGKPRFVAGSIGPSGLLPASEDPMLGKHHFREVVELFVPQVRGLMDGGVDV
                     LIIETVQDILELKAAIFAAGQVFEESGRKVPIQASITLDTSGRMLLGTDIAAALSILE
                     HLNVDVIGLNCSTGPDYMREPARYLGEYSTKPVSIIPNAGLPINVDGKAVFPMEPEPF
                     ARDLRDMVEGFGVGIVGGCCGTTPDHIGCIIQEVETRPVTRRPAKPRDQVASMIKAVD
                     LAQDPAPTIVGERVNTLGSRKVKRLVIADDLDGVLNVAVGQMEEGAHLLDVSVALTER
                     SDEADTMRKLVKKLALTVDGPLVIDTTEADVVQEALEQYPGRAVINSINMENGRERIE
                     KVMPLAMAHGAAVIALTIDEEGMAKTCERKLEVAKVIHRIVTEEHGLAPEDLIFDALT
                     FPLTTGDPEFVNSAVETIKGIRMIEEALPGVKSVLGISNVSFGINPAARKVINAVFLY
                     HCIKAGLDLAIIHPSHVVPFSEIPEEERKLAEDLVFNRTPDALQHVIEFYEGKTEEST
                     AGPDPMLEMEVRERLHYRIVHRKKEGVEADIDQAVEESGDPVDVLNNVLLPAMKEVGD
                     KFGAGELILPFVLQSAETMKKAVAQLENYLEKQEGATKGTVVLATVYGDVHDIGKNLV
                     NTILTNNGYTVHDLGKQVPISRIIDAAVEHNATAIGLSALLVSTSKQMPLCVQELHHQ
                     GHMFPVMIGGAAINPAYAHRSLFVDEDTTYEPGVFYAKDAFEGLSLMDRIIVPEARAQ
                     LIEETLEKARKTLNKPSRDSFIAPADAADTERSATPLVEPPAPPFWGVREMTDFTLAD
                     VWPHLDLKTLFRLHWGGKGVKGEAWERLQEEEFLPRLKAMQEDAEQSGWLQPCVRYGF
                     FPAKSAGNDLVIFGPEDHDREIERFTFPRQPARERLCLADYFQPIDSGKKDVVAFQAV
                     TMGKAASQRTEETQARGDYAESYFTHGLSVSSAEGLAEMVHQRVRAMLGIGEETGKRY
                     SWGYPSCPDLEQHVIVDRLLEMDEIGVEVTDGYQFNPEQTTAAIVVHHPDAKYFALLR
                     TGGDPAAEPASAAIAGD"
     gene            4404..4853
                     /locus_tag="VGR22_10695"
     CDS             4404..4853
                     /locus_tag="VGR22_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129075.1"
                     /translation="MNAGMRDLLSHIVARYATTEDERRIELDANAEESGADIEADKAA
                     AVERERQLASEQGDAFARGLTLAWERARRGGKEITFDDRQPDQNAMASALVDYLVRFD
                     LASSRSREVGDHHYLYDIAVDWDRLRDVAEENGQRLDDVFDTSHGAA"
     gene            4857..5696
                     /gene="rnc"
                     /locus_tag="VGR22_10700"
     CDS             4857..5696
                     /gene="rnc"
                     /locus_tag="VGR22_10700"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEV2129076.1"
                     /translation="MSDTAGISGQPMSNPSGTGRPTPFRLPEPEVEAALAARLGVTFE
                     NPLLLRQALTHRSVLHDWQQIEDIPVILQSNERLEFLGDAMLGAITAEYLYNRDPDAD
                     EGQLTRHRVAAVRAETLVRWARELHVPEALYLGTGEQITHSARDRMLAGAFEALVGAI
                     HLDRGREAAERFVLRFLEHDVDVILRQEALANPKGRLQEVLQERERVGPEYETIATAG
                     PDHAREFTVVVKVREELLGEGRGRSKREAQQMAAREALAHLGVVESPRVRIDRSGAGS
                     RLE"
     gene            5693..6715
                     /locus_tag="VGR22_10705"
     CDS             5693..6715
                     /locus_tag="VGR22_10705"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEV2129077.1"
                     /translation="MSRSYGEMNIFSGNSNPQLVEQICDYLGLRPGRADVFKFSNDNT
                     FVRIGESVRQHDVFVVQSFSTPVNDAIMEMLIMIDTLRRASAGRITAVIPYYGYGRTD
                     KKDQPRVPITARLVAKLIVQAGADRVLTIDLHAGQIQGFFDIPVDELSAMFLGARYFV
                     EKRLEKPVVVSPDLGNTKRSRNFAEILDAPLAIIEKRRIGNQDKAEMLNLIGSVEGRQ
                     VVIVDDEIDTAGSITQAAEVCLERGAEEVYATCTHPVLSGPAIERLRASAIREVVVTN
                     TVDVPPEKRFDRLTVISVAELLGEAIQRIHAGASVSSVYRSEGPYQRRMPFTVSSSAS
                     DDGVGD"
     gene            6830..8029
                     /locus_tag="VGR22_10710"
     CDS             6830..8029
                     /locus_tag="VGR22_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129078.1"
                     /translation="MTASAMRLSRRGFIIAGAVLATTTRLGIARTLGQASDIDLVSRL
                     GELLAAVPARDLTVDTPISFYYADLAGQLDALGIRQLPADRVPDDLPEGFVDATYALP
                     LASRAYQFAMTPAWLETFGFSPLAVEQILEIATPPGIITLFAGGYERDRVGAALASSG
                     YTEVLQEIGVSYWTFGDDVDLTTSVGQLGGGTMNQAVVSDDLLIFARQESDIQRVTWT
                     RAGYEPSMLEQGIWTGMMSVFSPDTVGLIPLAPTELNPAPASGIPLATPVTVEGRDSG
                     IEYLALGIRPGGRTAGLALVGEGTPEPIETPEGTAVPTSVEVRIRYTDPALAEREAAA
                     IPDRWASMSSLLTGQPYTDLMTVVDARVHEEDAQVVAIDFTADVPNVWIRLIHTLDLL
                     PFIPAEG"
     gene            complement(8067..8762)
                     /locus_tag="VGR22_10715"
     CDS             complement(8067..8762)
                     /locus_tag="VGR22_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129079.1"
                     /translation="MAAAPSSENQETSIYTADRETAAKPEMESRQEETSDDAAEQIET
                     EQPAASTEPSDTDLSGWPAPETSETRHDDINPESSHTGWDTTEPESDHGLHDSGPSEW
                     EGWSPTPSPESAVHPYDDNESLQEIRELVGTLQQRLDRLADPLPVGARGIDADELADQ
                     LSGWASGGPDSGELLEVIREARANPRDIDAVTRLAARAEELERLVERYGTICSDAQRW
                     VARLRYPDSSNGA"
     gene            9083..9739
                     /locus_tag="VGR22_10720"
     CDS             9083..9739
                     /locus_tag="VGR22_10720"
                     /inference="COORDINATES: protein motif:HMM:NF020905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HEV2129080.1"
                     /translation="MTNVLVGQRAVAAKQLASGAGAAFEVTAGQLLHIIDLAGGQVAS
                     MIAYSGRGHNETLSPTATMTINASLTLKQGSQLYSDACEPMFELVEDPVGRHDLLTMP
                     QPSDGTTTKARETSTTRDALSEAAESVGVYRPGLPSPVNFFKHVVVKQRGEIEVRDPF
                     SERNDAILLRVLKDAVVIIANAHKEKKPGISQAPGRNEKPGMMLVRVYQSAFSENGSA
                     "
     gene            complement(9867..9989)
                     /locus_tag="VGR22_10725"
     CDS             complement(9867..9989)
                     /locus_tag="VGR22_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129081.1"
                     /translation="MERIDRSMWRKHIRPQDLSALALVLAIALAVVVIVSVVLF"
     gene            10136..11098
                     /gene="hemH"
                     /locus_tag="VGR22_10730"
     CDS             10136..11098
                     /gene="hemH"
                     /locus_tag="VGR22_10730"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEV2129082.1"
                     /translation="MSYTPVAADEATHHLLIMAYGGPDKLEDVRPYLMDVRNYRPTSD
                     EIVHEITERYEQIGGRSPILELTRAEADGIEKSLNDLAGEGERWKVWVAMRHWHPFIK
                     DVLNEMEGAGVERAVGLVMAPHYSSMSIGAYFKRAEAAGSSVELAPVKSWNLNPGYLD
                     ALHDRIADALEKFPPEVRNDVPIIFTAHSLPERILQHNDPYPDELRATVDALAERLPG
                     REWHWAYQSAAMTADPWLGPDVGEVIEELAAQGRKHVLICPVGFICEHVEILFDIDIE
                     YQQLADKLGVHLERIEMLNDHPDMLAGLAQVARGRAVDAGWLNG"
     gene            11103..>11458
                     /locus_tag="VGR22_10735"
     CDS             11103..>11458
                     /locus_tag="VGR22_10735"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2129083.1"
                     /translation="MTCRVVVIGGGISGLAAAHHLLVNHDGLAVTVIERDVQLGGKVI
                     TYQRDGFVIEGGPDSMLSAKPRGVGLSRELGLGDRLVTTLERNRRSFVLRKGRLHRIP
                     EGLTGLVPTRLAPIARS"
BASE COUNT         1877 a         3691 c         3840 g         2050 t
ORIGIN      
        1 ctgggaacac catccggtct ggttccacgg cgacgtcgcc gtcggcaacc tgctgctcgg
       61 agatggtcgg ctgtgtgctg tcatcgactt cggcacctcc ggcgtgggcg accctgcgtg
      121 cgacctggca gtcgcatgga cgctcttcac gggcgagagc cgggaggcgt tccgcgccgc
      181 gctaccgttc gacgaggcaa cctgggcgcg cggccgggcc tgggcattgt ggaaggcgct
      241 catcctcgtc gccggcatcg ccgagaccaa cgcccgtgag gcggagaggt cccggcagat
      301 tatcgacgaa attcttgccg actaccggcg cgtcgggtag acaacgtcga gtagcccagc
      361 gtcgctagtg tgcgcagggt gtgtcgacag ggttcggttc caaggactgg tagcggatgg
      421 cttggccgct ccccgcgttg tgtcagactc gttcgcgcgg ccaatgtcgt cccttccgga
      481 ccggtttcct gccccgaacc tggccaaata cgctctgtgc ccaagacctg atagtttggc
      541 agccttgagt ggcaccactg gtggtcaagg ctgccaagat ggccagcggc caagttgctt
      601 cgtttgacag cctaacaata actagagttc gaaacgacga gcgggagagc gttggcaccc
      661 tcccgcttga tttgcaatga ccactgctag tcgcctgcga tggcggccga tgcgggctcg
      721 gccgccgggt cgccgccggt acggagcagg gcgaagtact tggcgtccgg atggtgcacc
      781 acgatcgcgg ctgttgtctg ctcggggttg aattggtagc cgtcggtcac ctcgaccccg
      841 atctcgtcca tctccagcag ccggtcgacg atcacgtgct gctccagatc cgggcaggac
      901 gggtagcccc aggagtagcg cttgcccgtc tcctcgccga tgccgagcat cgcccgcacc
      961 cgctggtgga ccatctccgc cagcccttcg gcactggaga cgctcaagcc gtgcgtgaag
     1021 tagctctcgg cgtagtcgcc gcgcgcctgg gtctcctcgg tgcgctgcga ggccgccttg
     1081 cccatcgtca cggcttggaa ggcgacgacg tccttcttgc cgctgtcgat cggctggaag
     1141 tagtccgcca ggcagagccg ctcgcgcgcc ggttgccgtg ggaaggtgaa ccgctcgatc
     1201 tcccggtcgt ggtcctcagg gccgaagatg accaggtcgt tgcccgcact tttggccggg
     1261 aagaagccgt agcgcacgca gggctgcagc cagccactct gctccgcgtc ctcctgcatc
     1321 gccttcaggc gcggcaggaa ctcctcctcc tgcagccgct cccacgcctc gcccttgacc
     1381 cccttgccac cccagtgcag gcggaagagc gtcttgaggt cgaggtgcgg ccaaacatcg
     1441 gcgagggtga agtcggtcat ctcgcgcacg ccccagaacg gcggcgccgg cggctcgacc
     1501 agcggcgtcg cactccgctc ggtgtctgcc gcatcagccg gggcgatgaa cgagtcgcgc
     1561 gacggcttgt tgagcgtctt gcgggccttc tccagcgtct cctcaatgag ctgagcacga
     1621 gcctccggca cgatgatcct gtccatgagg gagagcccct cgaaggcgtc tttggcgtag
     1681 aagacgcccg gctcgtaggt ggtgtcctca tcgacgaaga gcgagcggtg cgcgtaggcc
     1741 gggttgatcg ccgcgccgcc gatcatcacc gggaacatgt gcccctggtg gtgaagctcc
     1801 tgcacgcaga gcggcatctg cttgctggtc gaaaccagca gcgcggagag gccgatcgcg
     1861 gtggcattgt gctcgaccgc agcgtcgatg atccggctga tcggcacctg cttgccaagg
     1921 tcgtgcaccg tgtagccgtt gttggtgagg atcgtgttga cgaggttctt gccgatgtcg
     1981 tggacgtcgc cgtagaccgt ggccagcacc accgtgccct tggtcgcgcc ttcctgcttc
     2041 tcgagatagt tctcgagctg ggccaccgcc ttcttcatcg tctcggccga ctgcagcacg
     2101 aacggcagga tcagctcgcc ggctccgaac ttgtcgccga cctccttcat ggccggcagc
     2161 agcacgttgt tgagcacgtc gaccgggtcg ccgctctcct cgaccgcctg gtcgatgtcc
     2221 gcctcgacac cctccttctt gcggtggacg atgcggtagt gcagccgctc gcgaacctcc
     2281 atctccagca tcgggtccgg cccggcagta ctctcctcgg tcttgccttc gtagaactcg
     2341 atcacgtgct gcagcgcgtc cggcgtgcgg ttgaagacca ggtcctcggc gagcttgcgt
     2401 tcctcctctg gaatctcgct gaacggcacg acgtgcgagg gatggatgat cgccaggtcg
     2461 agccccgcct tgatgcagtg gtagaggaag acggcgttga tcaccttgcg ggccgccggg
     2521 ttgatgccga aggagacgtt ggagatgccg agcacgctct tcacgcctgg cagcgcctcc
     2581 tcgatcatgc ggatgccctt gatcgtttcc accgccgagt tgacgaactc cggatcgccg
     2641 gtggtgagcg ggaaggtgag ggcgtcgaaa atgaggtcct ccggcgcgag gccgtgctcc
     2701 tccgtcacga tgcggtggat gacctttgcc acctcgagct tgcgctcgca cgtcttggcc
     2761 atgccctcct cgtcgatcgt gagggcgatc acggcggcgc cgtgggccat cgccagcggc
     2821 atgactttct cgatgcgctc gcgaccgttc tccatgttga tggaattgat taccgcgcgg
     2881 cccgggtact gctccaacgc ctcctgcacc acgtcggcct cggtggtgtc gatcaccagc
     2941 gggccgtcga ccgtcagcgc gagcttcttg acgagcttgc gcatcgtgtc ggcctcgtcg
     3001 ctgcgctcgg tgagtgccac gctgacgtcg agcaggtgtg cgccctcctc catctgcccg
     3061 acagcgacgt tgagcacgcc gtcgagatcg tcggcgatca cgagccgctt gaccttgcgc
     3121 gagccaaggg tgttgacgcg ctcgccgacg atcgttgggg ccgggtcctg cgcgaggtcc
     3181 acggccttga tcatgctggc gacctggtcg cgtggcttcg ccggtcgcct ggtgactggt
     3241 cgcgtctcga cctcttggat gatgcacccg atgtggtcgg gcgtggtgcc gcaacagccg
     3301 ccgacgatgc ccacgccgaa gccctccacc atgtcgcgca ggtcgcgcgc gaacggctcc
     3361 ggctccatcg ggaagaccgc cttgccgtcg acgttgatcg gcagcccggc gttggggatg
     3421 atcgagacgg gcttcgtgct gtactccccg agataccggg ccggctcgcg catgtagtcc
     3481 ggaccggtgg agcagttgag accgatgaca tcgacgttga gatgctcgag gatgctgagc
     3541 gccgcggcga tatcggtgcc gagcagcatg cgtccggagg tgtcgagcgt gatggacgcc
     3601 tggatgggga ccttcctgcc ggactcctcg aagacctgcc ccgctgcgaa gatggcggcc
     3661 ttgagctcga ggatgtcctg caccgtctcg atgatgagca cgtcgacgcc gccatccatc
     3721 aggccgcgga cctgcgggac gaagagctcg accacctctc ggaagtggtg cttgccgagc
     3781 atcgggtcct cggaggcggg cagcaggccg gaagggccga tcgacccggc gacgaagcgc
     3841 ggcttgccct tcgtgctgta ctcgtccgca acacggcggg cgagcttcgc ggaggcaagg
     3901 ttgatatcgt acgtctgtgc gccgaggccg tactcctcgg ccttgatcac gctgctgccg
     3961 aaggtgttgg tctcgatcac atccgcgccg atctcgagga acgaggcgtg gatctgctgg
     4021 atcacctccg gcttcacgac cgagagatag tcgttgtagc cctcggtcgc ctgcccgccg
     4081 tagtcctctg gcgtgagccc gaggttgagg atggtggcgc ccatcgagcc gtcgtagaag
     4141 acggggcgag tgttgaccag gtcgagaaat cggctcatgt gtcgggaact cccggggtgt
     4201 gccgcagcgg cgcggggtcg gtcacgttcg gaaatggtac caactcgggg tggtagcccg
     4261 gtttatgtcc gggttcagga tagtatgaag cccactgatc cgtcggtact gggacacacg
     4321 gcggcagccc ggggtcctgc gccgtatact ttggcgggtg tgccatcgcc catgaccccc
     4381 gcagtactgc ctggaggcct ctcttgaacg ctggaatgcg cgatcttctc tcacacattg
     4441 ttgcgcgcta cgccactacc gaggacgagc gccggatcga gctcgacgcc aacgccgagg
     4501 agtccggcgc cgatatcgag gccgacaagg cagcggcggt cgaacgcgag cgccaactgg
     4561 ccagcgagca gggtgatgcc tttgccaggg ggctgacgct cgcatgggag cgcgcgcgcc
     4621 gcggcggcaa ggagatcacg ttcgatgatc gccagccgga tcagaatgcg atggccagcg
     4681 cgctcgtcga ctacctggtg cgcttcgacc ttgcgtcatc gcgttcccgc gaggtcggcg
     4741 atcaccacta cctctacgat atcgcagtgg actgggatcg cctgcgcgat gtcgcggagg
     4801 agaatggcca gcggctggac gacgtattcg acacctccca cggagcagcc tgagacgtga
     4861 gcgacacggc gggaatatcc ggccagccca tgtcgaatcc ctccgggacc ggtcgcccga
     4921 ccccctttcg gcttccggag cccgaggtcg aagcggccct tgccgcacgt ctgggtgtca
     4981 cctttgagaa cccgctgctg ctccggcagg cgctgaccca ccgctccgtg ctccatgact
     5041 ggcagcagat cgaggatatc cccgtgatcc tgcagagtaa cgagcgcctt gagttcctcg
     5101 gtgacgccat gctcggggcg atcaccgccg agtacctcta caatcgcgac cctgacgccg
     5161 acgaggggca gcttacccgg catcgggtgg cggccgtccg cgccgagaca ctcgtgcgat
     5221 gggcgcgcga gctgcacgtg ccggaggcgc tctatctcgg caccggcgag cagatcaccc
     5281 acagcgcgcg cgaccgtatg ctggccggtg cgttcgaggc gctcgtaggg gccatccatc
     5341 tcgatcgggg gcgcgaggcg gcggaacggt tcgtgcttcg tttcctcgag cacgatgtcg
     5401 acgtgattct ccgacaggag gcgctcgcga accccaaggg gcggttgcag gaggtgctcc
     5461 aggagcgcga gcgtgtcggg ccggagtatg agacgatcgc gacggccggt cccgaccacg
     5521 cgcgcgagtt cacggtcgtc gtcaaggtcc gtgaagagct ccttggcgag ggacgcgggc
     5581 ggagcaagcg ggaggcacag cagatggcgg cccgcgaggc gctggcacac cttggggttg
     5641 tcgagtcacc gcgagtccgg attgaccggt caggagcagg gagcagactg gagtgagccg
     5701 atcatatggc gagatgaaca ttttcagcgg gaactccaat ccgcagctcg tggagcagat
     5761 ctgtgactat ctcggtctcc ggccgggccg ggcggacgtc ttcaagttct ccaacgacaa
     5821 caccttcgtg cgaatcggcg aaagcgtgcg ccagcatgac gtcttcgtgg ttcagtcatt
     5881 ctccacccca gtgaacgatg cgatcatgga gatgctgatc atgatcgata cgctgaggcg
     5941 tgcatctgcc ggtcgcatca ccgccgtgat cccgtattac ggttacggcc gcaccgacaa
     6001 gaaggaccag ccccgcgtgc cgatcacggc gcggctcgtt gccaagctga tcgtgcaggc
     6061 gggcgctgac cgcgtgctga cgatcgacct ccatgccggg cagattcagg gctttttcga
     6121 catccccgtg gacgagttga gcgcgatgtt cctcggtgcg cgctacttcg tggagaagcg
     6181 gctggagaag ccggtggtgg tctcgccgga cctcggcaac accaagcgct cgcgcaactt
     6241 cgccgagatt ctcgacgcgc cgctggcaat catcgagaag cggcgtatcg gcaatcagga
     6301 caaggccgag atgctcaatc tgatcggctc cgtcgagggg cggcaggtgg tgatcgtcga
     6361 cgacgagatc gacaccgccg ggtcgatcac ccaggcggcg gaggtctgtc tcgagcgtgg
     6421 tgcagaggag gtctacgcga cctgcacgca cccggtactg agtggtccgg cgatcgaacg
     6481 gctcagggcg agcgcgatcc gggaggtcgt ggtgacgaac acggtcgatg tgccgcccga
     6541 gaagcggttc gatcgcctga cggtgatctc ggtggcggag ttgcttggtg aggcgatcca
     6601 gcgcattcac gcgggcgcca gcgtctcctc cgtctaccgc tcggaggggc catatcagcg
     6661 tcgcatgccg ttcaccgtca gcagcagcgc cagcgacgac ggcgtggggg actgacgcaa
     6721 gcggctgcgg agtagcgcac gcctgatctt gacgggctcc ggtgacacgg tggatgcttc
     6781 gcccaggtcc cagtatgttg cccgattcac ccagctctca ggaggctcca tgactgcctc
     6841 ggcaatgcgc ctctcccgac gcggattcat catcgccggc gccgtcctcg ccaccaccac
     6901 ccggctcggg atcgcccgaa cactcggaca ggccagtgat atcgacctcg tcagccgcct
     6961 gggcgagttg ctggccgccg tcccggctcg tgatctcacg gttgacacac cgatctcctt
     7021 ttactatgcc gatctcgccg ggcaactcga cgcgctcggc attcggcaat tgcccgccga
     7081 ccgggtcccg gacgacctgc ccgagggctt cgtcgatgcc acctacgcgc ttccgctcgc
     7141 ctcccgggca taccagtttg ccatgacgcc ggcgtggctc gagaccttcg gcttcagtcc
     7201 gctcgcggtt gagcagatac tggagatcgc caccccgccg ggaatcatca cgctctttgc
     7261 cggcgggtac gagcgggatc gtgtcggggc cgccctcgcg tccagcgggt acacggaagt
     7321 gctgcaggaa atcggtgtta gctattggac cttcggcgat gacgtcgatc tgacgacatc
     7381 ggtcggacag ctggggggcg ggacgatgaa ccaagcggtt gtcagcgacg accttctgat
     7441 cttcgcccgg caggagagtg acatccagcg ggtgacctgg acccgggcgg ggtacgagcc
     7501 atcgatgctg gaacagggga tctggaccgg gatgatgtcg gtcttctcgc ccgacaccgt
     7561 gggactgatc ccgctggccc ccaccgaact caacccggcc cctgcgtctg ggatcccgtt
     7621 ggcgactccg gtcaccgtag agggccgcga cagcggcatc gagtacctcg cgctcgggat
     7681 tcgccccggg ggacgaacgg ccgggctcgc attggtcgga gagggaaccc cggagccgat
     7741 cgagacgccg gagggcacgg cggttccgac gtcggtggaa gtgcgcatcc ggtacaccga
     7801 tcccgcactg gccgagcgag aggcagcagc aatccccgat cgatgggcct cgatgtcatc
     7861 gctgctgacc gggcagccct acaccgacct gatgaccgtc gtggatgccc gcgtgcacga
     7921 ggaggacgcg caggtagtgg cgatcgactt caccgccgac gtgccgaacg tctggatccg
     7981 gctgatccac acgctcgacc tgctaccgtt catccccgcc gagggatgag acagcgacca
     8041 gtcctagtgt cggctgggcg gcccggtcag gcgccgttgg agctgtctgg gtagcggaga
     8101 cgggcgaccc agcgctgggc gtcgctgcag atcgtgccgt agcgctccac caaccgctcc
     8161 agctcctccg cacgggcagc gagacgcgtc actgcgtcga tatcccgcgg gttggcccgc
     8221 gcctcgcgga tcacctccag cagctcgcca ctgtcaggtc ccccactggc ccagccgctg
     8281 agctgatcgg caagctcatc agcgtcgatc ccacgcgcac cgaccggcaa ggggtcagcc
     8341 agccggtcga gtcgctgctg cagcgtgccg accagctcac ggatctcctg cagagactcg
     8401 ttgtcgtcgt atgggtgcac cgctgactct ggtgacggcg tcggcgacca gccctcccat
     8461 tcggatggtc cggagtcatg caggccatgg tccgactcgg gttcggtggt gtcccagccg
     8521 gtgtggctgc tctccggatt gatgtcatca tgccgtgttt cagacgtctc gggtgctggc
     8581 cagccggaga gatcggtgtc gctcggctca gtgctggcgg ctggctgctc ggtctcgatc
     8641 tgttcggccg cgtcgtccga ggtctcttcc tgccgggact ccatctcagg cttagccgcg
     8701 gtctcgcggt ccgcagtgta gatggaggtc tcctggttct cactggatgg tgccgcggcc
     8761 atggtctctc caggctccag ccgaacccca caggaagtgc agaatttggc gcccccgcgg
     8821 acggccgcgc cacagttcgt gcagtactgc attccggaac tcctcttccc gcgagtcgtg
     8881 cgtcacggtg ccgtgtggac tggtggcgac acggcgtttt ccgcgagtgt acgcgatcgg
     8941 atgacgtgcc ggggctgcgc cggttggact gcctgccttg catcctgcga caatggatgg
     9001 caattgcgtg ggcccgaaga ggacgacatc acggacatcg ggtgtcgctg gatgggccat
     9061 ggagtgagga agtaccaacg tcatgacgaa tgttctggtg ggacagcgcg ccgtggccgc
     9121 gaaacagctc gcgagcggcg cgggtgccgc cttcgaggtc accgccggcc agctgctgca
     9181 catcatcgat ctcgccggcg ggcaggtggc cagcatgatc gcctattccg gccgggggca
     9241 caacgaaacc ctctcgccga cggcgacgat gaccatcaac gcctccctga cgctcaagca
     9301 gggcagccaa ctctacagtg acgcgtgcga gccaatgttc gagctggtcg aggatccggt
     9361 cgggcgccac gatctgctga ctatgccgca gccatcggac ggcaccacaa ccaaggcgcg
     9421 ggagacctcc accacgcggg acgcgctcag cgaggcggcg gaatcggtcg gtgtctaccg
     9481 gccagggctg ccgtcgccgg tcaacttctt caagcacgtg gtggtcaagc agcgcggtga
     9541 gatcgaggtg cgggatccgt tttccgagcg gaacgacgcg atcctgctgc gggtgctgaa
     9601 ggatgcagtg gtgattatcg ccaacgccca caaggagaaa aagccgggca tctcgcaggc
     9661 gccgggccgc aacgagaagc cggggatgat gctcgtgcgg gtctaccaga gcgccttcag
     9721 tgagaacgga agcgcctgat tggccaaagc tggtttcccg tcatgccccg acacgtctcg
     9781 gcgcttgagc gcctcaccgg ctcagcatga cagtggggtt ggcggccacg ggtagcttct
     9841 cacgttgcgc tgcgctgcgg tcttcgttag aacagcacaa cgctgacgat cacgacgacg
     9901 gcgagggcga tggccagcac cagcgccagc gccgagagat cctgtggccg aatgtgcttt
     9961 cgccacatcg agcggtcgat ccgttccatc ccgatctgtc tcctcaggcc agacagccga
    10021 acggtgtcag gacgttcccg acggtatgat gctccgggat atggtagcca tacggatgga
    10081 acacatccgt cggctgccgc ctgtcggcag ctgtcgatga aaggccctga ttcgcatgtc
    10141 ctacacacct gtcgccgcag acgaggcgac gcaccacctg ctcattatgg cgtacggggg
    10201 tcccgacaag ctcgaggatg tccgcccgta cctgatggac gttcgcaact accggccgac
    10261 gtcggacgag atcgtccacg agatcaccga gcgctacgag cagatcggtg ggcgatcgcc
    10321 gatcctcgag ctgacccggg ccgaggcaga cggcatcgag aagtcgctca acgacctcgc
    10381 tggcgaaggc gagcggtgga aggtctgggt ggcgatgcgt cactggcatc cattcatcaa
    10441 ggacgtgctc aatgagatgg aaggggctgg tgtggagcgc gcggtcggtc tggtgatggc
    10501 cccgcactac tcctcgatga gcatcggcgc ctacttcaag cgtgccgagg cggccggcag
    10561 ctcggttgag ctggcgccag tgaagagctg gaatctcaat cccggctatc tcgacgccct
    10621 gcacgatcgc atcgccgacg cactggagaa gttcccgcca gaggtgcgga acgacgtacc
    10681 catcatcttc actgcgcaca gcctgcccga gcgcatcctg cagcacaatg atccgtatcc
    10741 ggacgagctg cgggccacgg tcgacgcgct ggcagagcgg ctgccgggcc gtgagtggca
    10801 ttgggcctac cagagcgcgg caatgaccgc cgacccctgg ctggggccgg atgtcggcga
    10861 ggtgatcgag gagctcgcgg cgcagggcag gaagcacgtg ctgatctgtc cggttgggtt
    10921 catctgcgag cacgtcgaga tcctcttcga catcgacatc gaataccagc agcttgccga
    10981 caagctcggt gtgcatctcg agcggatcga gatgctcaac gaccatcccg acatgctggc
    11041 cgggctcgcg caggtcgcgc gtggccgtgc cgtcgatgcc ggctggttga acggatagca
    11101 ccatgacctg tcgtgtggtc gtcatcggtg gaggtatttc gggactggcg gcggcgcatc
    11161 acctgctcgt caaccacgac ggccttgcgg tcaccgtgat cgagcgcgac gtgcagctcg
    11221 gtggcaaggt catcacctac cagcgggacg gctttgtcat cgagggcggg ccggactcca
    11281 tgctgagcgc caagccgcgt ggcgtcgggc tcagccggga gctggggttg ggagaccggc
    11341 tcgtcacgac actggagcgc aatcggcgct ccttcgtgct ccgcaagggc aggctccacc
    11401 gcattcccga ggggctgacc ggtctggtcc cgacccgtct cgcgccgatc gcgagatc
//
LOCUS       DASYWL010000304         6003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_428683, whole genome shotgun sequence.
ACCESSION   DASYWL010000304 DASYWL010000000
VERSION     DASYWL010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6003
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428683"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="VGR22_10740"
     CDS             <1..1018
                     /locus_tag="VGR22_10740"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEV2129084.1"
                     /translation="DQRPGEYASVILAAAIKEGHEGPVMIQGDHYQASAKDFAEDPEK
                     EIGAVRDLAIEAMKAGYGNIDVDASTLVDLSLDTLREQQRENAVQTAAITRAIRDVEP
                     EDITVSIGGEIGEVGKENSTVEDLDAFMTGYLEELRRHSDEVGRDLVGISKISVQTGT
                     SHGGVVLPDGSIADVSVDFETLAALSTATKDRYDMGGAVQHGASTLPEEAFGKFAEAD
                     AVEVHLATAFQNVLLDHAQFPDGLRQRLHAWLDENRASERKPDQTDAQFYYTTRKRVF
                     GPFKKEFWSLPDEVKAAIGRDLRERFELIMRELKVAGNANLVDQHIKRVDVPLTAPEA
                     LTQR"
     gene            complement(1342..2046)
                     /locus_tag="VGR22_10745"
     CDS             complement(1342..2046)
                     /locus_tag="VGR22_10745"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV2129085.1"
                     /translation="MRDRIDQLTLTFTYPNPGEETPLITLHDHTGHALGCISTRDALP
                     CLPLKDESVRRVIALDVLEHVHDEQAWLAELARVLARGGEVAVRVPLENWMAWADALN
                     VSRYVADTTGIGAEPFETIPTGWHRHYAPGDLPALLKLAGFEVEAISTEGTPVLEIPH
                     LAALLVGNVIGDHKPQQRVSRWLKRFRTGPRLPLPAKIAQTITVRARKQRQGFRPDPD
                     LDHTHRPEHESTEPLE"
     gene            2207..2944
                     /locus_tag="VGR22_10750"
     CDS             2207..2944
                     /locus_tag="VGR22_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129086.1"
                     /translation="MSLHPIDDSVVTRFMAAVTGDAPPEAAWQTWWPDRIPVLLERMR
                     AGDERAANEITLRLAYALGTEHPTFAAPGFGLSLWEARIDRGVGMYLRPPSRLFRDFG
                     LELDVLRTMPIRIDTNLGLMGGAFIPAHLVPKVKELLETREQRMARRLREAEYDPVVA
                     LTLFNEAIDYALEHGMGLYEALDAVGPEGQAAFNGEVVIGSRDRVPKAERKRIEELSK
                     PPKKPGLIQRMLGRSGSESANGHMPGE"
     gene            3093..4142
                     /gene="hrcA"
                     /locus_tag="VGR22_10755"
     CDS             3093..4142
                     /gene="hrcA"
                     /locus_tag="VGR22_10755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEV2129087.1"
                     /translation="MVSTTPDPTTSILTERQRTILKLVVKEYVASGRPVGSKTLTERY
                     DVGVSPATIRNEMAELERLGFVQQLHTSGGRVPTDRGYRFFVHHLMGAPELPSGDQIM
                     IRHQFRQVEVQLDQWVDFAASVLAETAGSVSIVTAPRTVIARLRHFELISLQPQLALL
                     ILVTQESAVRQMMLHLPSEYDQAQLSRLADALVPSVRGLSSDEIADRLQGASDLARYV
                     LEHVVTGLRGLDSVDRTQIRHSGIENILGEPEFAGADAWSVLELLRGGTLLSAVLPAL
                     TSGDDVQVFIGNENPADALQRFGVVMASYGVDGEVTGVLGVLGPTRMPYWRSISSVRY
                     MARLMSDLMEDLYSA"
     gene            4198..4797
                     /locus_tag="VGR22_10760"
     CDS             4198..4797
                     /locus_tag="VGR22_10760"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEV2129088.1"
                     /translation="MTNNNKDQQRVAAEEQELNGQHTEALETDEPGAEPAVDEQKELE
                     TVRAERDRYLDQLQRSVAEFTNYRRRSEQERAQLVPIVRRDVLLQFLPVMDDLERAMA
                     QIPEDKWSDGWVTGLVMIESKFRNLLERADVTPIDPVGEPFDPSRHEAVATEPGTDGS
                     TVVEVYQKGYAIGDVLIRPAMVKTGAPQDEDGETPRAEA"
     gene            4928..>6003
                     /locus_tag="VGR22_10765"
     CDS             4928..>6003
                     /locus_tag="VGR22_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEV2129089.1"
                     /translation="MARTIGIDLGTTNSVMATIEGGEPVVIPNAEGERITPSVVAVTS
                     NGERLVGRFARRQAVTNPDNTVASIKRFMGRKFDDPAVARDKELVTYELRKADNGDVQ
                     VKMGEKWYSPQEISAMILQKLKTDAEAYLGDTVDKAVITVPAYFDNAQRDATKDAGRI
                     AGLDVQRIINEPTASALAYGLDKKEDEKVAVYDLGGGTYDISILDLSEGVFQVMATNG
                     DTHLGGDDFDQVIIDWLAEEFKKQEGIDLRNDRMALQRLREAAEKAKIELSSTQQTDI
                     NLPFITADASGPKHLNVTLSRSKLESLVGGLIDRTVPPMEGALKDAGISKGEVDEALL
                     VGGMTRMPAVQRKVKEFFGKEPHKG"
BASE COUNT         1093 a         1868 c         2044 g          998 t
ORIGIN      
        1 cgaccagcgt cccggcgagt atgccagcgt gatcctcgcg gccgcgatca aggaggggca
       61 cgaggggccg gtgatgatcc agggcgacca ttaccaggcc agtgccaagg acttcgccga
      121 ggaccccgag aaggagatcg gcgcggttcg cgacctggcg atcgaggcga tgaaggccgg
      181 atacgggaac atcgacgtcg acgcctcgac gctcgttgac ctgagcctgg acacactgcg
      241 cgagcagcag cgcgagaatg ccgtccagac cgccgcgatc acccgtgcca tccgtgatgt
      301 ggagccggag gacatcaccg tttccatcgg gggcgagatc ggcgaggtcg gcaaggaaaa
      361 cagcaccgtc gaggacctcg acgccttcat gaccgggtat ctcgaggagt tgcggcgcca
      421 cagcgacgag gtcgggcgcg atctggtcgg catcagcaag atcagcgtgc agaccggcac
      481 cagccacggc ggcgtcgtgt tgccagacgg ctccattgcc gacgtcagcg tcgacttcga
      541 gacgcttgcc gcgctttcga cggccaccaa ggatcgctac gacatgggcg gcgcggtgca
      601 gcatggcgcc tcgacgctgc ccgaggaggc cttcggcaag ttcgccgagg cggatgcggt
      661 cgaggtccat ctcgccaccg ccttccagaa cgtgctcctc gaccatgcgc agttccctga
      721 tggcttgcgc caacgcctgc acgcctggct ggacgagaac cgcgccagcg agcgcaagcc
      781 ggatcagacg gacgcgcagt tctactacac gacgcggaag cgggtctttg ggccattcaa
      841 gaaggaattt tggtcgctgc cggacgaggt gaaggcggcg atcggcagag acctgcgcga
      901 gcggttcgag ctgatcatgc gcgagctcaa ggtggccgga aacgctaacc tggtcgacca
      961 gcacatcaag cgggttgacg tcccgctgac cgcgcccgag gcgttgacgc aacgctagtg
     1021 ctacggtttg aggaccggcc gggggacgtg tcgacacgtg acccgctatt ggcagcggtg
     1081 atagacctgc cccagcagcc cgccacgccg ccgtttgggg atcgtcatgt tatgtcgatc
     1141 atgttcggca aacgccgacc atgcggaaca agcgcccgca gcatgtatca tccgcattgt
     1201 ctgcggagtg cgtgagttgt ggatatcaag ggacaaacgc cgggcacagg gccaggcgct
     1261 acaggaccgc tccgatacat gccctgtagc gccacctcct gtgggcggcg ctggccatca
     1321 gcccactctg attgcctgtg gctactccag cggctcggtg ctttcgtgtt ccgggcggtg
     1381 ggtgtgatca agatccgggt cgggacggaa tccttgccgt tgcttcctcg cccggacggt
     1441 gattgtctgg gcaatcttcg ctggcagcgg cagacgtggt cccgtccgga atcgcttcag
     1501 ccacctgctc actcgttgct gtggcttgtg gtcgccgatc acgttgccga ccagcagtgc
     1561 cgcaagatgc ggaatctcca gcactggcgt tccctccgtg gagattgcct ccacctcgaa
     1621 tcctgccagc ttcagcaggg ctggcagatc gcctggcgcg tagtgccggt gccagccagt
     1681 gggaatggtc tcgaacggct ccgcgccgat tccggtcgtg tcggcaacgt agcgggacac
     1741 gttgagggcg tcggcccatg ccatccagtt ttccagcggc acgcgaacgg ctacctctcc
     1801 cccgcgcgcc agcacccgcg ccagctcggc cagccacgcc tgctcgtcgt gcacgtgctc
     1861 cagcacgtcg agcgcgatca cacgccgcac cgactcgtcc ttgagcggca ggcatgggag
     1921 agcatcgcgt gtcgagatgc atccgagggc atgccctgta tggtcgtgca gggtgatgag
     1981 cggcgtctcc tcacccgggt tggggtaggt gaaggtcagc gtcaactggt cgattcgatc
     2041 gcgcatacgg tgtcctcacg agcggtgggg cggccaccca gaccacgttg gcggcgaccg
     2101 ccacacgtat aatgccacca cccggagaga cgaggtcgcc gatcggccgc cgcatgcgcg
     2161 aagttgtcgc ggcccaccac gatgaaggtc cgttgcgatg gagatcatgt ccctgcaccc
     2221 aatcgacgac tcggtggtga cccgcttcat ggccgccgtc accggtgacg caccccccga
     2281 ggctgcctgg caaacgtggt ggcccgaccg gattccggtg ctgctggagc gcatgcgcgc
     2341 gggtgacgag cgtgctgcga acgagataac cctccggctt gcctacgcgc tcggcacgga
     2401 gcatccgacg tttgccgcgc ctgggttcgg cctcagtctc tgggaggcgc ggatcgaccg
     2461 cggcgtcggg atgtacctcc gaccaccgtc acgcctcttc cgtgacttcg gacttgagct
     2521 cgacgtgctg cgcacgatgc cgattcgcat cgacaccaac ctcgggctga tgggcggcgc
     2581 cttcatcccg gcgcacttgg taccgaaggt caaggagctg ctggagacac gcgagcagcg
     2641 catggctcgc cggctgcggg aggcggagta tgacccggtg gtcgccctga cgctcttcaa
     2701 cgaggcgatc gactacgcgc tggagcatgg tatggggctg tacgaggcgc tcgatgctgt
     2761 tgggcccgag ggacaggcgg cgttcaacgg cgaggtggtg atcggcagtc gcgatcgcgt
     2821 gccgaaggcc gagcgcaagc gcatcgagga gctcagcaag ccgcctaaga agccgggcct
     2881 catccagcgg atgctgggac gcagcggttc cgagagtgcc aacggacaca tgccgggcga
     2941 gtaggtcgac acacccgaaa aacccgagaa accggcgagc tggagccatt gagccccctc
     3001 gcaaccttga caaggggcgt atcagacggc tatgctctcg tcggttagca ctctcctgtc
     3061 gggagtgcta atgtatggca ggaggtgcga ggatggtgag tacgacgcca gatccgacaa
     3121 ccagcatttt gactgagcgt cagcggacga ttttgaagct ggtcgtgaag gagtatgtcg
     3181 cgtcgggccg accggtcggg tcgaagacgc tgacggagcg ctacgacgtg ggcgtctcgc
     3241 cggcaacgat ccggaacgag atggccgagc tggagcggct gggcttcgtg cagcagctgc
     3301 acacctccgg cgggcgggtt ccgaccgacc ggggctaccg ctttttcgtc catcacctga
     3361 tgggtgcccc ggagttgccg tcgggcgatc agatcatgat tcgccaccag ttccgccaag
     3421 tcgaggtgca gctcgatcag tgggtcgact tcgctgcctc ggttctcgcg gaaacggcgg
     3481 gctcggtctc gatcgtgacc gcgccgcgaa ccgtgatcgc gcggctgcgc catttcgagc
     3541 tgatctcgct gcagccgcag ctggcgctgc tcatcctcgt cacgcaggag agcgcggtgc
     3601 gccagatgat gctgcatctg ccatccgagt atgaccaggc gcagctgagc cggttggccg
     3661 acgccctagt gcccagcgtg cgcggtctga gttccgatga gatcgcggac cgcctgcagg
     3721 gtgcgtcgga cctggcccga tacgtgcttg agcatgttgt cacgggtctg cgcgggctcg
     3781 actcggtcga ccggacgcag attcgccact ccggcatcga gaacatcctc ggtgagccgg
     3841 agttcgccgg cgctgacgcc tggtcggtgc tggagctact gcgcgggggc accctgttga
     3901 gcgcggtgct gccggcgctg accagcggcg acgacgtgca ggtgttcatc ggcaacgaga
     3961 acccggccga cgcgctgcag cgtttcggcg tcgtgatggc cagctacggg gtcgacggcg
     4021 aggtcactgg cgtcctcggg gtgctgggcc cgacccgcat gccctactgg cgctcgatct
     4081 cgtcggttcg ctatatggct cgattgatga gtgacttgat ggaagatctg tattcggcct
     4141 gattcacagg caacgcattc gttgcgacgg aaggcgtggt ggcgaatgga gaacgatgtg
     4201 acgaacaaca acaaggatca gcagcgggtg gccgccgagg agcaggagct caacggccag
     4261 cacaccgagg cactcgagac ggacgaaccc ggggcggagc ctgcggttga cgagcagaag
     4321 gagctggaga cggtccgcgc ggagcgtgac cggtatctcg atcagcttca gcggtcggtg
     4381 gccgagttca ccaattaccg ccgtcgttcc gagcaggagc gggcacagtt ggtgcccatc
     4441 gttcggcggg atgtgctgtt gcagttcctg cccgtaatgg acgacctgga gcgcgcgatg
     4501 gcgcagattc cggaggacaa gtggtctgac ggctgggtca ccggcttggt gatgatcgag
     4561 tccaagtttc gcaacctgct ggagcgggcc gacgtgactc caatcgatcc ggtcggcgag
     4621 ccgttcgatc cgagccggca tgaggcggtc gcgaccgagc cgggcaccga tggctccacc
     4681 gtcgtcgagg tctaccagaa gggctatgcg atcggcgatg tgctgattcg cccggcgatg
     4741 gtcaagaccg gcgcgcctca ggatgaggac ggggagacgc cccgcgcgga ggcctgacag
     4801 caggttaaat cccagcgctc tgccggcggc gcgcggcgag cgcggtcacg aacatcttgc
     4861 ggtcgcgcca tttgatgagc cgaagtcgac atcggcacac acgtgaatag atcaggagta
     4921 tcgagacatg gcacggacaa tcggtattga cctcggaacg accaactcgg tcatggccac
     4981 catcgagggt ggcgagccgg tcgtcatccc gaacgctgag ggcgagcgca tcactccctc
     5041 ggttgtcgcc gtcacgagca acggcgagcg gctggtgggg cgcttcgcgc ggcgccaggc
     5101 ggtgaccaat ccggacaaca cggttgcgtc gatcaagcgg ttcatgggac gcaagttcga
     5161 tgatccggca gtcgcacgcg acaaggaact ggtgacctac gagctgcgca aggccgacaa
     5221 cggcgacgtg caggtcaaga tgggcgagaa gtggtacagc ccgcaggaga tctccgcgat
     5281 gatcctgcag aagctgaaga cggacgccga ggcgtacctc ggtgacacgg tcgacaaggc
     5341 cgtgatcaca gtcccggcct acttcgacaa cgcccagcgc gacgccacca aggacgccgg
     5401 tcgtatcgct ggcctggatg tgcagcgcat catcaacgag ccgactgcct ccgccctggc
     5461 ctacggtctg gacaagaagg aggacgagaa ggttgccgtc tacgacctcg gtggcggtac
     5521 ctacgacatc tcgatcctcg acctgtccga gggcgtcttc caagtgatgg ccaccaacgg
     5581 tgatacgcac ctcggtggtg atgacttcga ccaggtgatc atcgactggc ttgccgagga
     5641 gttcaagaag caggagggca tcgacctccg caacgatcgc atggccctcc agcgcctccg
     5701 cgaggccgcc gagaaggcca agatcgagct ctccagcacg caacagaccg acatcaacct
     5761 gccgttcatc acggcggatg ccagcgggcc gaagcacctc aacgtcacgc tcagccgctc
     5821 gaagctggag tcgctggtcg gcggtctgat cgaccgcacc gtcccgccga tggagggcgc
     5881 gcttaaggac gccggcatct ccaagggcga ggtcgacgag gcgctgctcg tcggtggcat
     5941 gacgcgcatg ccggccgtgc agcgcaaggt caaggagttc ttcggcaagg agccgcacaa
     6001 ggg
//
LOCUS       DASYWL010000305        16558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_353682, whole genome shotgun sequence.
ACCESSION   DASYWL010000305 DASYWL010000000
VERSION     DASYWL010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 16558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16558
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353682"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="VGR22_10770"
     CDS             <1..1160
                     /locus_tag="VGR22_10770"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEV2129090.1"
                     /translation="PTIVGSRLMYRSSPEYFAQFAQRAVEAGATIVGGCCGTTPEHIA
                     AMRTALDTVPKETTGDGATAAPTLQPVRTRHPADGQAAEPTQLKQMLGKKFLKCVEID
                     PPKGFNPQKAIDGARLLKDAGVHAINVADSPMARVRMSAMTLCYLIQHEVDVETIIHF
                     TTRDRSLMGLQSELLGAHAAGIRNILALTGDPPSLGDYPDSSAVYDVDSIGLIRIINR
                     LNSGEDSAGASIGRGANFTIACAIDPTRDDLEQEARRLHEKIEAGVDFVMTQPIFDVK
                     VWQRLLKIYGADRLPVPVMIGVLPLQSSRHAEFLHNEVPGITLTDAARDRMRRAGSEG
                     RREGIRMAQELLTELVPYADGTYLMPSFGRYEVAAEVISILEDEPVLATTH"
     gene            1281..1643
                     /gene="rplS"
                     /locus_tag="VGR22_10775"
     CDS             1281..1643
                     /gene="rplS"
                     /locus_tag="VGR22_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEV2129091.1"
                     /translation="MAVDVIRSIEQEQVKEMPEFGPGDTVRVHAKVVEGNRERVQVFE
                     GVVIRRRNGGLNENFTVRRIAAHGIGVERTFLLHSPRLDRVEVVRHGRVRRAKLYYLR
                     GRTGRAARIVERRRDLNR"
     gene            1731..2378
                     /locus_tag="VGR22_10780"
     CDS             1731..2378
                     /locus_tag="VGR22_10780"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEV2129092.1"
                     /translation="MTDSPNLLAWETCLGKAGYQTVVGLDEVGRGALAGPIIAAAVVL
                     PPDVDLTSGMWSDICDSKVISSARRETLAAWIAERAARCAVAALEAPVIDEIGIGPAN
                     RLVMEMALGKIERMCEVDFLLLDALTIDHAAPQSGIIDGDALSLSIAAASIVAKVHRD
                     AIMTELSATWKAYGWDSNKGYGVARHLQALRQHGPCEHHRFSYRPVRDAGAPSYA"
     gene            2371..2751
                     /locus_tag="VGR22_10785"
     CDS             2371..2751
                     /locus_tag="VGR22_10785"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEV2129093.1"
                     /translation="MPDPSIRKRRGQTGESHARAWLEGHGYRRVASNWHCRSGELDLV
                     MIDGTELVFVEVKTRTGERAGRASEAVSRDKRRKIPVSAEWFIATRPACQDMIWRCDI
                     AAVTINPDTGTAHIDHFENAVVSG"
     gene            2793..3251
                     /locus_tag="VGR22_10790"
     CDS             2793..3251
                     /locus_tag="VGR22_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129094.1"
                     /translation="MIDRPPSVSRTTDDIGIGDELARSGDWRSAIAVWQSAAARDATC
                     RESVERRLTWFLEQTRGSPKPLESRSVRLVIAAAMSGLLGTMLVLAAGEPGSPYSNLL
                     AAVAWVLFIVSATMAVIAARRRQQPELDTMLRRARATADRLDTSHKDCTA"
     gene            3248..3787
                     /locus_tag="VGR22_10795"
     CDS             3248..3787
                     /locus_tag="VGR22_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEV2129095.1"
                     /translation="MNDPSNRLPQSPADEHRDEAPDNACIAVLTMSDTRTREEDHSGE
                     IVRQGLIWRGFTVAAYEIVPDDPNQIREQIAAWLADGTVDAIITNGGTGISSRDSTFE
                     VVDALLEKRLDGFGELFRMLSWQEIGAAAMLSRAIAGSVGSKAIFCLPGSSNGVKLAM
                     EKLIGPELGHVIHELRKHR"
     gene            3827..5179
                     /gene="hisS"
                     /locus_tag="VGR22_10800"
     CDS             3827..5179
                     /gene="hisS"
                     /locus_tag="VGR22_10800"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEV2129096.1"
                     /translation="MAKQDARILKGFRDYLPDDMILRQKLTEIFRGVFEKHGFEPIDT
                     PTLEYLDVLTGKAGENETLMYHFEDHGGRKVGLRYDLTVPLARFIAMHENEVVLPFKR
                     YHIAPVWRAEKPQRGRFREFIQCDADIVGSSSMLADAEGISVLADAIYAVGLPQAVIS
                     INHRKLLEAVARLAGVPAEHAGGVFRAVDKLDKIGPEGVRAELVRIGVIEAAADQVLA
                     LVAEQGEPDELLDNAGRRLADIDGGPQAVQDLRDLVRHLADLGVHRHSWKIDLSLARG
                     IDYYTGPVCEARVESPRVGSIAGTGRYDGLVGNFLGRQIPATGISLGFERILEVVREH
                     DLLDVPEAIADAFMVYLPECIGEASRIVRELREEGLRIDQSIIDNKGIGAQLKYADRR
                     GIPYAIIPGSEELDQGEVSLKNLRNGDQTRVAISVLAEELRHRYAEPAPDSGRKAGRT
                     "
     gene            5176..5877
                     /locus_tag="VGR22_10805"
     CDS             5176..5877
                     /locus_tag="VGR22_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEV2129097.1"
                     /translation="MTEDQETYQRYLWIVAHPDDAEFSSAGTIHKIASEGKDVVIVQA
                     TSGDRGTDNPEWTPETLGAAREKEELEAAKRLGVKKVEFLHEGDGHVMPTVEFREKVV
                     RMIRTYKPDVLVTHDPFRPYALHPDHRGVGITAHDCVYPLARDPLYFPEHSEAGLEPH
                     KTAEIWYFGSEAPDMVVDITDHFEAKIEALKAHETQLGNAEGLSERLRERAQELAKDE
                     PFELGEAFKTVQMRR"
     gene            5892..7088
                     /locus_tag="VGR22_10810"
     CDS             5892..7088
                     /locus_tag="VGR22_10810"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HEV2129098.1"
                     /translation="MDAALTSFAHAGKQVFGQQWQPSRVAIAPGRVELLGNHVDYNGG
                     LVLAGAIDRVIHAVGGRADVDEDMLLLHAPDVSPSIAEVSISHPVIEDPEAPTTPGDY
                     LAGIVQALREARVPARGGQAIVVAGDVPVGFGMSSSAALCVSLTLLVAAAPLEDASIV
                     TVARHAEHLTGAPVGAMDQSASVAGGVILFDGSTNDVVQLDPDLGDHVFAVADSGVHH
                     ALNESQYPQRVRESQEALELLRANAGVDIQFLGELTPEQWGAIVAGDPYWLSPTPRKR
                     VQHVVSEVERVREGVKAIQSGDWPRFGELMTASGRSSAEEYEISHPAVEELVSLLRDQ
                     PGVLGARMMGGGEGGPALALLRRDAVESVREALTREFFDPHHMDAVAAFEICSFGPGA
                     RSMDLP"
     gene            complement(7139..8104)
                     /locus_tag="VGR22_10815"
     CDS             complement(7139..8104)
                     /locus_tag="VGR22_10815"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEV2129099.1"
                     /translation="MREPIRSLVISLPAPDDYVENLRREFPQIEITRVEPDELDDALT
                     EADAVLAWRVPEEAIQNAPKLKWIHRGAAGVEDLITPTLRESSIVLTNSSGVHAPNMA
                     EHVLACMLAFARRLPKLLTMQRDHTWKGQRIVGVFELTDQTVTLAGLGDIGQAVAVRA
                     KAFGMNTLGVRRDTALPRPEGVDTIMSTDRLHEALQSADHVVNSLPLTESTRGMFDAD
                     AFAAMRPGTYFYNVGRGKTVDTAALIDALERGRIAGAALDVTDPEPLPEDSPLWDMPE
                     VIITGHTAGKTPHMWDRVFRLFAQNLQRYEAGDPMLNVVDIHAGY"
     gene            complement(8295..8996)
                     /locus_tag="VGR22_10820"
     CDS             complement(8295..8996)
                     /locus_tag="VGR22_10820"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2129100.1"
                     /translation="MSWDEAFADRYEDWSAHMTADIAFYVELAGAADGPLVELAVGNG
                     RVAIPVAQATGQRVIGIHFSPAMLAKARIRAAEAGVELDLREGDMRDIALDEPAALIY
                     CPFRALLHLPTWSDRRRTFEHVAASLRPGGRFVWNAFAFDHGVAARLDGERQDEPVPH
                     TVRYAVGDNRIDLVLDSGATSSLWWATKNEWLGLIDVAGLEVEALYGGFAREPFSDES
                     REYVFVVRRVSGATL"
     gene            complement(9024..10442)
                     /locus_tag="VGR22_10825"
     CDS             complement(9024..10442)
                     /locus_tag="VGR22_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEV2129101.1"
                     /translation="MATAQAQGGWFDNQFRMSERGTNIRTELMAGLTTFMVMSYIIAV
                     NPAILSFNGELSVTQVATVTCLVAGVMTILMGLYANIAIAIAPGLGLNAIVAFTLVGS
                     MGLTFPQAMGVVVAEGIIITILVLTGVRRYVMDVVPMALKQAISVGIGFFILFIGLRN
                     AGLVTFISGTDAPVGTTTGLLELTPLDTWPIFVVMVGLLVTIAFIARGHQAALLLGII
                     AATVTAFVVDVAEWPAEPMGGPDFELLGNFSFGFIGELGVLTALLVVLSLMLADFFDT
                     MGTLIGVGSQAGYLDERGNLPNAQKPLLVDSLAAVAGGAASSSSATSYIESSAGVGVG
                     GRTGLVSVTTGVLFLLALPFVELVNAIPGVATAPALIVVGVLMVGVLGSEAVSSAADS
                     AWERRGINFSDVEESLPVVMTMLIMPLTFNITNGIGAGFVTWVLMKVAAGKGGQVHPA
                     LYAIAAAFLLYFLRWALFDATF"
     gene            complement(10649..11674)
                     /gene="moaA"
                     /locus_tag="VGR22_10830"
     CDS             complement(10649..11674)
                     /gene="moaA"
                     /locus_tag="VGR22_10830"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEV2129102.1"
                     /translation="MAVPVQIHPLVKASQGPRDSYGRAMNYLRISLTDRCNFRCVYCM
                     PAVGMQFQPREELLTDDELIRLVGLFARLGVNRLRLTGGEPTVRPHLVDLIREMKRFD
                     GIDEISMTTNALILNRIAGDLKEAGLDRINVSIDTLDPVKFRMMTRGGRLDLVWEGIK
                     AADEAGMKPIKLNTVVLKNHNEHDVHEMAALTLEHDWHIRFLEIMPMEGVGIVYDEGL
                     VTSAETQANLERRFGPLEPIETHPSDPARLWKIPGAQGSIGFISPISAPFCDACNRVR
                     LTADGKLRLCLIRPDEVDFRDMMRAGASDEEILQTMRAGIWRKPWGHRVHEGERNTIR
                     GMSQIGG"
     gene            complement(11788..13410)
                     /locus_tag="VGR22_10835"
     CDS             complement(11788..13410)
                     /locus_tag="VGR22_10835"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEV2129103.1"
                     /translation="MDAVELRTLDGPNLFLLRPAIKLELRASDEERSALDPEILGLVR
                     ERVDVPQDASGRIDIAKQLAGVVAWLHAELDMGLAEIVVRELEEEGHAAVAFSWDRRR
                     ASKVLGHLAWRLVAGEAADLEAELGEIRALLTAEPDASDLPEMWPDSKRTVPTIGITG
                     TNGKTTTTRLVASILRHAGNKVGWSSSSGVLIDGEMVLSGDYTGPSGAARVFEEPGID
                     YAVVETARGGILLRGLGYEHNDVSVMTNISADHMGLHGVYSLDVLGEVKAVVARVTRE
                     DGFAVLNADDLRVLRVRDVIVARPFLFTRNPEHADVRRHVGVGGWALVVENNAIVWYH
                     DGEHEVLAELDAVPVTFGGRAQHMVENALAAAAACLGVRLSAEQVREGLAAFHNRPDQ
                     NRGRLNVYEVDGATVVVDFAHNEAGLEHLLAFARGFCDSSGKLYAVIGTAGDRDEAAI
                     TGIARIAATRADRVIIKDSERYLRGREAGEMLDLMRPVVGEVLVDETGNEREGFFSGL
                     RRLEPGDVLAVMCVEDYETILPYLDERATSLS"
     gene            complement(13445..14368)
                     /locus_tag="VGR22_10840"
     CDS             complement(13445..14368)
                     /locus_tag="VGR22_10840"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEV2129104.1"
                     /translation="MDFLLTLSVLLIILAAVYGVLLWARKAETDRSANVGLYLLYGIP
                     GLLLTVTGVALLANGHEDGWFVLAFGLAFSLPLIRGFRKLLSYVTPLDPASPIDMMGL
                     GVIFALFAYFSYAMSLGPADEPIEGVTVGAQLINVLFFVAVAYVAVGTGNYRNVDEVT
                     ARLGFTRPTLQDFAVGAAAVVPTFGLSILGSMLTLWFQPDRFEDLGETMEEMASGTDF
                     TIVSLLIFASAGIGEEILFRGAIQPRFGIVITAALWALVHTQYQLTFVVLGLFLAGIL
                     FGLIRKYMSTTPAIIAHALYNAAVVALQGVG"
     gene            14552..15493
                     /locus_tag="VGR22_10845"
     CDS             14552..15493
                     /locus_tag="VGR22_10845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEV2129105.1"
                     /translation="MAADRRETGKRQQRARRGGLGKGLDALIPARQEPEVARPDGSGA
                     AEQRSGSTELPIDSIAPNPWQPRSHLDHQHLEELTASIRTHGIVQPLIITPADEPDRY
                     HLIAGERRWRAAQRAGLTSVPAIIKSATPQAMLELALVENIVRADLNPIEEAEAFRQL
                     IEEFGMSQAELAGRVGRSRVAVTNTLRLLNAPDAIREALLRGHVTEGHARALLGLPGA
                     ADQTAALQTVIEQQMSVRQTEQLVRRWNTSDRKPQEPRERDPEERRVEEEFRQALGTN
                     VAFRRRTGGNGGTLTIQVYSDEEMNALYSRLIGDDSW"
     gene            complement(15677..16393)
                     /locus_tag="VGR22_10850"
     CDS             complement(15677..16393)
                     /locus_tag="VGR22_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129106.1"
                     /translation="MTEQTTESTLTAAERTLVALFFIVVIVVSVIVVGRLGTEPTTVI
                     GPVEERPAVDAEVPRPVPVRDTVDIASDWAEEWDDEAWPILVSAQFSYPPDPGNATPV
                     ADEGTVLVTFAAPKEDGEWPRLTLAVGRQSGVIYHEDEITSTVEPPEPIGNLIADLPI
                     SAEQAFRVAQEVVGEEYRSGCELSRRHVQVVLDTTDREEPHWVVVYYDERERTTNDIV
                     VRIDAQTGATETEIRDDLSC"
BASE COUNT         3088 a         5547 c         5218 g         2705 t
ORIGIN      
        1 acccgaccat cgtcggctcc cgcctgatgt accgctcatc gcccgagtac ttcgcccaat
       61 tcgcgcagcg tgccgtcgag gccggtgcga ccatcgtcgg cggctgctgc ggcacaacgc
      121 cggagcacat cgccgccatg cgaacggcgc tcgacaccgt gccgaaggag accaccgggg
      181 acggtgcgac ggcagctccc acccttcagc cggtgcgaac cagacacccg gcggacggcc
      241 aggctgcaga gccgacgcag ctcaagcaaa tgctcggcaa gaagtttctc aagtgcgtgg
      301 agatcgaccc gcccaagggg ttcaacccgc agaaggccat cgacggcgca aggctgctca
      361 aggacgcggg tgtccatgcg atcaatgtcg cggacagccc gatggcccgg gtgcggatga
      421 gcgcgatgac cctctgctac ctgatccagc atgaggtcga cgtcgagacg atcatccatt
      481 tcaccacccg cgaccggtcc ctcatggggc tccagtctga gctgctcggt gcgcacgccg
      541 ccggtatccg caacatcctg gcgctgaccg gcgatccgcc cagcctgggc gactaccctg
      601 actcctcggc ggtctacgac gtcgactcga tcggcctgat ccggatcatc aaccggctca
      661 acagcggcga ggattccgct ggagcgtcga tcggccgtgg cgccaacttc accattgcct
      721 gtgcgatcga cccgacgcgt gatgatctcg aacaggaggc gcgccgcctc cacgaaaaga
      781 tcgaggcagg cgtcgacttc gtcatgaccc agcccatctt cgacgtgaag gtgtggcagc
      841 ggctcctgaa gatctatggc gcggatcgcc tgccggtccc cgtcatgatc ggcgtgctgc
      901 cgctgcagtc ctcccgccat gccgagttcc tgcacaacga ggtgccaggc atcacgctca
      961 ccgacgcagc acgtgaccgg atgcgccgcg ccgggtcgga gggccgccgc gagggcatcc
     1021 gcatggctca ggagttgctg accgaattgg tgccatatgc ggacggcacc tacctgatgc
     1081 cgtcgttcgg ccgctacgag gtcgccgccg aagtgatcag catcctcgag gacgagccgg
     1141 tactggcaac aacacactga cgtgctacaa tgcctcggtg cccgccgcag tcgggatgtg
     1201 tggcgtgtgc gcatttcgcg cgagagacca acgaattcgg ggcgcgacaa tccccggcag
     1261 caggcaagga acggcgacag atggcagtgg acgtgattcg ctcgatcgag caggaacaag
     1321 tcaaggagat gccggagttc ggacccggtg acaccgtgcg tgtacacgcg aaggtggtcg
     1381 agggcaaccg tgaacgggtg caggtgttcg agggcgtggt gatccgccgg cgcaacggcg
     1441 gcctcaacga gaacttcacc gttcgccgca tcgctgccca cggaatcggt gtcgagcgca
     1501 ccttcctcct ccactccccg cgccttgaca gggtcgaggt ggtccgtcac gggcgcgttc
     1561 gccgcgccaa gctctactac ctgcgaggtc gcaccggacg cgcggcccgc atcgtcgagc
     1621 gtcgccgcga cctcaacagg tagcgcagcg cggctcgcag atcgccaaga aaccgtggtc
     1681 gggtacagtt gtacccgacc acggtttctt gtttgcggag gcgccacatc gtgacggaca
     1741 gccccaatct gctcgcctgg gaaacctgtc tcggcaaggc cggctaccaa acggttgtcg
     1801 ggctcgacga agtaggccgc ggggcgctcg ccgggccgat catcgccgcc gcggtcgtcc
     1861 tgccgcctga cgtcgacctc acctccggga tgtggagcga catctgcgat tccaaagtca
     1921 tcagctcggc tcgccgggag acgctggctg cgtggattgc cgagcgggca gcacgctgcg
     1981 ccgtcgccgc cttggaggcg ccagtgatcg acgagatcgg cataggacca gccaaccgac
     2041 tcgtgatgga gatggccctc ggcaagatcg agcgcatgtg cgaggtggat ttccttctcc
     2101 tcgacgccct caccatcgat cacgctgctc cacagagtgg gatcattgac ggcgatgccc
     2161 tctccctctc catcgcggca gcatcgatcg tcgccaaggt tcaccgtgac gcgatcatga
     2221 ccgagctatc tgctacctgg aaggcgtacg ggtgggacag caacaagggg tatggcgtcg
     2281 ccagacacct gcaagcgctt cgccagcacg gcccctgcga gcaccatcgg ttctcctacc
     2341 gtccggtacg cgatgcggga gctccgagtt atgcctgacc cgagcatacg caagcgccgt
     2401 gggcagaccg gcgagtcgca cgcacgcgcc tggcttgagg gccacggcta ccggcgtgta
     2461 gcgagcaact ggcactgccg gtcgggagag ctcgacctcg tcatgatcga cggcactgag
     2521 ctggtctttg tcgaagtcaa gacgcgcacc ggcgagcggg ctgggcgggc ttcggaggcg
     2581 gtctcacggg acaagcggcg gaagatccca gtctcagctg agtggttcat cgccacccgt
     2641 cctgcatgtc aggacatgat ctggcgctgc gacatcgctg ccgtaaccat caaccctgac
     2701 accggcaccg cgcatatcga tcatttcgag aacgcggtcg tcagcggctg atcggttagg
     2761 ccggctgctc cgaatccggt accattgcat gtatgatcga tcggccacca tctgtatcgc
     2821 ggacaactga cgacatcgga atcggtgacg agcttgcgcg tagcggcgac tggcgcagcg
     2881 cgatcgccgt gtggcagagc gccgcggcgc gcgatgcgac ctgccgggaa tcagtcgaac
     2941 gccgcctgac gtggtttctg gagcagacgc gaggatcgcc gaagcccctg gaaagccgct
     3001 cggtgcggct ggtgatcgct gcagctatga gtgggctgct cgggactatg ctggtgctgg
     3061 ccgcgggcga acccggttcg ccgtacagta acctgctcgc cgcggtcgcc tgggtgctct
     3121 tcatcgtgtc agcgacaatg gcggtgatcg ccgcgcgacg acggcagcag cccgaactgg
     3181 acacgatgct gcgacgggcc cgtgccaccg ccgaccggct ggatacctcc cacaaggact
     3241 gcacggcatg aacgatcctt ccaatcggct accccagtcg cccgccgacg aacaccgtga
     3301 cgaggcgccg gataacgctt gcatcgccgt gctcaccatg agcgatacgc gcacccgcga
     3361 ggaggaccac agcggcgaga tcgtgcgcca aggtctcatc tggcggggct tcaccgtcgc
     3421 cgcctacgag atcgtgccgg acgaccccaa ccagatccgt gagcagatcg cggcgtggct
     3481 cgctgatggc accgttgacg caattatcac caatggcggc accggcatct ccagtcgcga
     3541 ctccacattt gaggtcgtcg acgcgctgct ggagaagcgc ctcgacggct tcggcgaact
     3601 cttccgcatg ctctcgtggc aggagatcgg ggcggcggcg atgctctccc gggcaatcgc
     3661 cgggagcgtc gggtcgaaag cgatcttctg cctgcccggg tcctccaacg gcgtcaagct
     3721 ggcgatggaa aagctgatcg gtccggaact tgggcacgtg atccacgagc tgcggaagca
     3781 tcgctaagat gaacagcacc gccgaacacc ggcgcgagga gcaccaatgg cgaaacagga
     3841 cgcgcgcatt ctgaagggtt tccgggacta cctaccggac gacatgatcc tgcggcagaa
     3901 gctcacggag atcttcaggg gcgtcttcga gaagcacggg ttcgaaccca tcgacacgcc
     3961 cacactcgag taccttgatg tgctcacggg caaggccggc gagaacgaaa ccctgatgta
     4021 ccacttcgag gaccacggcg ggcgcaaggt cgggctccgc tacgacctca ccgtgccgct
     4081 ggctcgcttc atcgccatgc acgagaacga ggtcgtgctg ccgttcaagc gctaccacat
     4141 cgctccggtc tggcgggccg agaaaccgca gcggggtcga ttccgcgagt tcatccagtg
     4201 cgacgccgac atcgttggct cctcgtcgat gctggccgat gcggagggca tcagtgtact
     4261 cgccgacgcg atctacgccg tcgggctgcc gcaagccgtc atctcgatca accaccgcaa
     4321 gctgctggag gcagtggccc ggctcgccgg tgtgccggcg gagcacgccg gcggggtctt
     4381 ccgggccgtc gacaagctgg acaagatcgg tcccgagggg gtgcgcgccg aactggtcag
     4441 gatcggggtg attgaagcgg cggccgatca ggtgctcgcc ctcgtcgccg agcagggtga
     4501 accggatgag cttctggaca atgccggtcg gcgactcgcc gacatcgacg gcggcccgca
     4561 ggcggtgcag gacctccgag acctcgttcg ccatctcgcc gatctcgggg tgcatcggca
     4621 cagctggaag atcgatctct ccctcgcccg cggcatcgac tactacacgg gacccgtctg
     4681 cgaggcgcgc gtcgaatcgc ccagggttgg ctccatcgcc ggcaccggcc gctacgacgg
     4741 tctcgtcggt aacttcctcg gccgccagat ccccgcgacc ggcatctcgc tcggcttcga
     4801 gcgcatcctc gaggtcgtcc gcgagcacga tctgctcgat gttccggaag ctatcgccga
     4861 cgcgttcatg gtctacctgc cagagtgcat cggcgaggcc tcgcgcatcg tgcgcgagct
     4921 ccgtgaggag ggcctgcgga tcgaccagtc gatcatcgac aacaagggaa tcggcgcaca
     4981 gctcaagtac gccgaccgcc gaggcatccc ctacgcaatc attccgggca gcgaagagct
     5041 tgaccagggg gaggtctcac tcaagaatct tcggaatggt gaccagacac gcgtcgcgat
     5101 ctccgtactg gccgaagagc ttcggcaccg gtatgcggag cccgcgcccg attcaggcag
     5161 gaaagcaggc cgcacgtgac agaggatcag gaaacctacc agcgatacct gtggatcgtc
     5221 gcccatccgg atgacgccga gttctcatct gcaggcacaa ttcacaaaat cgcctccgag
     5281 ggcaaggacg tggtcatcgt gcaggcgacg tcaggcgatc gcggcaccga caacccggaa
     5341 tggacaccgg agacgcttgg agcggcccgc gagaaggagg aactggaggc ggcgaagcgt
     5401 ctcggcgtga agaaggtcga gttcctccat gaaggcgacg gacatgtcat gcccaccgtc
     5461 gagttccgcg aaaaagttgt gcggatgatt cgcacctaca agccggacgt gctcgtgacc
     5521 cacgacccct tccggcccta cgcgctccac cctgaccacc gcggcgtcgg catcaccgcc
     5581 cacgactgcg tctacccgct ggcacgcgac ccgctctact tcccagagca ctccgaggca
     5641 gggctggagc ctcacaagac cgccgagatc tggtatttcg gctcagaggc cccagacatg
     5701 gtcgtcgaca tcacggatca cttcgaggcc aagattgagg cgctcaaggc ccacgaaacg
     5761 cagctcggca atgccgaggg actttccgag cgacttcgcg agcgcgcaca ggaactggcg
     5821 aaagacgagc ccttcgaact tggcgaggcg ttcaagaccg tccagatgcg acgctagcga
     5881 ggagatccgc gatggatgct gccctcacct ccttcgccca cgccgggaag caggttttcg
     5941 gacagcaatg gcagccatca cgtgtcgcca ttgctcctgg ccgtgtcgag ctcctgggca
     6001 accatgtcga ctacaacggc ggcctcgtgc tggccggggc gatcgaccgc gtgattcatg
     6061 ccgttggtgg tcgcgccgat gtcgatgagg acatgctcct gttgcacgcg cccgatgtgt
     6121 ccccgagcat cgcggaagtt tccatcagcc atccggtgat cgaggatccg gaagcaccca
     6181 ctacaccggg cgactacctc gccgggatcg tccaggctct gcgagaggct cgcgtgccag
     6241 cccgcggcgg acaggcaatc gtcgtcgccg gcgatgtccc ggtcggcttc ggcatgagct
     6301 catcggcggc gctgtgcgtc tcgctcaccc tcctcgtcgc cgctgcaccg ctcgaggatg
     6361 cctcgatcgt caccgtggcg cgtcacgccg agcacctgac cggtgccccg gttggcgcga
     6421 tggatcagtc cgcctccgtg gccggaggcg tcatcctttt cgacggctcg accaatgatg
     6481 ttgtacagct cgatcccgat ctcggcgacc acgtcttcgc cgtcgccgac agcggggtgc
     6541 accatgcgct gaacgagtcg cagtacccgc agcgagtccg cgagagccag gaagcgctgg
     6601 aactgctccg cgccaatgcc ggcgtcgata tccagttcct cggcgagctg acaccggagc
     6661 aatggggagc gatcgttgca ggcgaccctt attggctctc gcccacgcca cgaaagcggg
     6721 tgcagcatgt ggtgagtgag gtggagcggg tgcgtgaggg cgtcaaggcc atccagtccg
     6781 gtgactggcc gaggttcggc gagctgatga ccgcatctgg gcgctcctcg gcggaggagt
     6841 acgagatcag ccatcccgcg gtcgaggagc tcgtgtcgct gctgcgcgat cagccaggcg
     6901 tgctcggcgc gcggatgatg ggcgggggcg agggcggacc ggcgctggca ctgctccgtc
     6961 gcgacgccgt cgaaagcgtg cgcgaggcgc tcacccgtga attcttcgat ccgcaccaca
     7021 tggacgcggt cgccgcgttt gagatttgct ccttcggccc cggtgcacgg agcatggatc
     7081 tgccctgacg cagtagcggg cgcaacactt tcgtcctgca gcttctcgcc tgctgcgatc
     7141 agtaccctgc atggatgtcc acgacattga gcatcggatc tccggcctcg taacgctgca
     7201 gattctgcgc gaacaaccgg aaaacccgat cccacatgtg cggcgtcttt ccagccgtgt
     7261 ggccggtaat gatcacttcc ggcatgtccc acaggggcga gtcctccggc agcggctctg
     7321 gatcggtcac gtcgagggcg gcgcccgcga tgcgaccgcg ctccagcgca tcgataagcg
     7381 ccgccgtgtc gaccgtcttg cctcgcccaa cgttgtagaa ataggttccc gggcgcatcg
     7441 ccgcaaaggc gtcggcgtcg aacatccccc gcgtcgattc ggtcagcgga aggctgttga
     7501 cgacatggtc ggcgctttgg agcgcctcgt ggagccggtc cgtggacatg atcgtgtcga
     7561 caccctcggg acgcggcagg gcggtgtccc gccggacgcc gagcgtgttc atcccgaagg
     7621 ccttcgcgcg aaccgcgacc gcctggccga tgtcaccgag ccccgcgagg gtaacggtct
     7681 gatccgtgag ctcgaagacg ccgacaatgc gctggccctt ccaagtgtga tcacgctgca
     7741 tggtcagcag cttcggcagt cggcgagcga aggccagcat gcaggccagc acatgctcgg
     7801 ccatgttcgg cgcgtgcacc cccgagctgt tggtgaggac gatgctgctc tcgcgcagcg
     7861 tcggcgtgat caggtcctcg acccccgccg cgccgcggtg gatccacttc agcttcggcg
     7921 cgttctggat tgcctcttcc ggcacccgcc aggcaagcac cgcatccgcc tcggtcaggg
     7981 cgtcatcgag ctcgtccggc tcgacccggg tgatctcgat ctgcggaaac tcgcgccgca
     8041 ggttttcgac gtagtcatcg ggtgcaggca gcgaaatgac gagggagcga atgggctctc
     8101 gcatctggtc gcctttctta tcagacggac gcggggatcc cggcggggcc ccgcatcgtc
     8161 aaagcgtcgg gtgtcactgc cgaccagcat accagcccga tacaccggcc ctggcatggc
     8221 tggcaatggc tcgcgaagag gtatcaggcc catcaggtcc gcggccgccc cgacaagcgc
     8281 caacgcgggc agtatcagag agtggcgccc gatactcggc gaacgacgaa cacatattcg
     8341 cggctctcgt cggagaacgg ctcgcgggcg aagccaccgt agagtgcctc cacctcaagt
     8401 ccggcgacgt caataagccc aagccactcg ttctttgtgg cccaccaaag cgaactcgtc
     8461 gcaccgctat ctaggactaa atcgatccgg ttatcaccca cagcgtaccg gacagtatgc
     8521 ggaaccggct cgtcctgacg ctcgccatcg aggcgcgcgg cgacgccgtg gtcgaaggcg
     8581 aaggcgttcc agacgaaccg cccgcccggc cggagcgagg cggcaacatg ctcgaaggtg
     8641 cggcgccggt cggaccaagt aggtaggtgc aagagggctc ggaagggaca gtagatcagg
     8701 gccgctggct cgtcgagggc gatgtcgcgc atgtcaccct cgcgcaggtc gagttccacg
     8761 cccgcttcag ctgccctgat acgggccttc gctagcatgg cgggcgagaa gtggataccg
     8821 atcactcgct ggccggttgc ctgcgcgacc ggtatcgcca cccgcccgtt cccgacggcg
     8881 agctcgacca gcggcccgtc tgccgcacct gcgagttcaa cgtagaaggc gatgtcggcc
     8941 gtcatatgag ccgaccagtc ttcgtaacgg tccgcaaatg cctcgtccca actcatccct
     9001 cgtactccca ccctgtgacg agactagaac gtcgcgtcga agagggccca gcgcaggaag
     9061 tacagcagga atgccgctgc gatcgcgtag agcgccgggt gcacctgtcc gcccttgcca
     9121 gcggctacct tcatcagcac ccaggtcacg aacccagcgc cgatgccgtt ggtgatgttg
     9181 aaggtcagcg gcatgatcaa catggtcatg accacgggca gcgactcctc gacgtcggag
     9241 aagttgatgc cgcggcgctc ccatgcgctg tccgccgccg aggagaccgc ctcgctcccg
     9301 agcacgccaa ccatgagcac gccgacaacg atcagcgccg gtgccgtcgc cacacccgga
     9361 atcgcgttca ccagttcgac gaacggcagc gccagcagga agagcacgcc ggtggtgacc
     9421 gacaccaatc cggtgcggcc accgacacca acgcctgccg acgattcgat gtagctcgtc
     9481 gccgacgagg aactggccgc gccgccagcc acggcagcca gcgagtcgac aagcagcggc
     9541 ttctgcgcgt tcggcaggtt cccgcgttcg tcgaggtagc cagcctggct cccgacgccg
     9601 atcagcgtgc ccatggtgtc gaagaagtcg gcgagcatga ggctgaggac caccaacaac
     9661 gccgtgagaa cgcccagttc cccaatgaag ccgaagctga agttgccgag cagctcgaaa
     9721 tccggacctc ccattggctc agccggccac tcggcgacat cgacgacgaa agccgtcacg
     9781 gtcgccgcga taatcccgag gagcagtgcc gcctgatggc ctcgcgcgat gaacgcaatg
     9841 gtgaccaaca gtccgaccat caccacgaag atcggccatg tatccagtgg cgtgagctcg
     9901 agcaagccgg tggtcgttcc gactggagcg tcagtgccgc taatgaatgt caccaatccg
     9961 gcgttcctga gcccaatgaa caggataaag aagccgatac cgacactgat cgcctgcttc
    10021 agtgccattg gcacgacatc catcacatac ctgcgcacgc ctgtgagcac aaggatcgtg
    10081 atgatgatgc cttccgccac caccacgccc atcgcctgag gaaaggtcaa ccccatgctg
    10141 cccaccaacg tgaaggcgac gatcgcattg agaccgagcc ctggcgcgat ggcgatcgca
    10201 atgttggcat agagccccat caggatcgtc atgactcccg ccaccaggca ggtgaccgta
    10261 gcgacctgcg tcacggagag ctcgccgttg aacgacagga tcgcagggtt cacggcgatg
    10321 atgtaggaca tcaccataaa ggtcgtgagc ccggccatca gctcggtgcg gatgttggtt
    10381 ccccgctcgg acatccggaa ctgattgtcg aaccagcccc cttgcgcttg tgctgttgcc
    10441 acgtctcgcc ttcccttcag tctcttggtt caatccgtgc tgcacagagc gccgggctcc
    10501 ccacccggca tgtacgtaca ctttacccca gaaaggggca cgacgaaacc gcagtcactc
    10561 ccggtttgcg acgctccacg caccggaatg cccccgaccg gctcgccgga ggcattcaca
    10621 tacattctcg cctgctcacg gctacgcgct agccgccgat ctgagacatg ccccggatgg
    10681 tgttgcgctc gccctcgtgg acccgatggc cccacggctt acgccagatt ccagcacgca
    10741 tcgtttggag gatctcctcg tcggaggcac cggcgcgcat catgtcgcgg aagtcgacct
    10801 catcgggtcg gatcaggcag agccgcagct tcccgtcggc tgtgagccgg actcggttgc
    10861 aggcgtcgca gaacggtgcc gagatcggcg agatgaaccc gatggatccc tgcgcgccgg
    10921 ggatcttcca gaggcgcgcc ggatcggaag ggtgggtctc gatcggctcc agtggcccga
    10981 atcggcgctc gaggtttgcc tgcgtctctg cggaggtcac gagcccttcg tcgtagacga
    11041 tgcccacgcc ctccatcggc atgatctcga ggaagcggat gtgccagtcg tgctccagcg
    11101 tcagcgcggc catctcatgg acatcatgct cattgtggtt cttgagcacc acggtgttga
    11161 gtttgatcgg cttcatgccg gcctcatcgg ctgccttgat gccctcccag acgagatcga
    11221 gccgccctcc tcgggtcatc atcctgaact tgacgggatc gagcgtgtcg atgctgacgt
    11281 tgatgcgatc gaggccggcc tccttcaggt cgccggcgat ccggttgagg atcaacgcat
    11341 tggtggtcat cgagatttca tcgataccat cgaagcgctt catctctcga atgaggtcga
    11401 cgagatgcgg gcgcacggtc ggctcgccgc cggtcagccg gagccgattg acgccgagtc
    11461 tcgcaaagag cccgacgagg cggatcaact cgtcgtcggt gagcagttcc tcgcgcggct
    11521 ggaactgcat gccgaccgcg ggcatgcagt agacgcagcg gaagttgcag cggtccgtga
    11581 gtgaaatccg caggtagttc atggcccgtc cgtaggaatc acgcggaccc tggctggctt
    11641 tcacgagcgg atggatctga accgggacgg ccatgcacct gatatcccct gccgaccgaa
    11701 cgcggttcaa ccggcctagt caaagtctga atatacgaat ccagcctacc aacgggggac
    11761 cgaagcgtca acaactgacg ccgcctttca gctcagggag gttgcgcgct cgtcgaggta
    11821 ggggaggata gtctcgtagt cctcaacaca catcacagca agcacgtcgc cgggctcgag
    11881 ccttcgcagg cccgaaaaga acccctcgcg ctcgttgccg gtctcgtcca ccaggacctc
    11941 accgacgacc ggccgcatca ggtcgagcat ctcgcctgcc tcgcggcccc gcaagtagcg
    12001 ctcggagtcc ttgatgatga ccctgtcggc ccgtgtggcg gcaatccggg cgatgccggt
    12061 aatagcggcc tcgtcgcggt caccggctgt cccgatcacc gcatagagct tcccgctcga
    12121 atcacagaac ccgcgcgcga aggccagcag gtgctccagc cccgcctcgt tgtgggcgaa
    12181 atcgaccacg acagtggcgc catcgacctc gtagacgttg aggcggcccc gattttggtc
    12241 aggacggttg tggaatgccg ccaagccctc ccgaacctgc tcggcagaca atctgacgcc
    12301 gagacacgcc gccgccgccg ccagcgcgtt ctccaccatg tgctgcgctc ggccaccgaa
    12361 ggtaaccggc actgcgtcga gctcggccag gacctcgtgc tctccatcgt ggtaccagac
    12421 gatcgcattg ttctcgacca ccagagccca gccccccacg ccaacgtggc gccgcacatc
    12481 cgcgtgctcg gggttgcgag tgaaaaggaa tggccgagca acgatcacat cccgcacccg
    12541 cagcacgcga agatcgtcag cgttcaggac ggcgaaccca tcctcacggg tgacacgcgc
    12601 gaccaccgcc ttgacctcgc caagcacgtc gagcgaataa accccgtgga gccccatatg
    12661 atcggccgag atattggtca tcacactcac gtcgttgtgt tcgtacccga gcccgcgcag
    12721 gaggatgccg ccgcgcgcgg tctccaccac cgcgtagtcg attcccggct cctcgaagac
    12781 gcgcgcggca ccggaagggc cggtgtaatc cccggacaac accatctccc cgtcgatcag
    12841 gaccccggat gaggacgacc acccgacctt gttgcccgcg tgacgcagga tcgacgcgac
    12901 cagccgcgtc gtggtggtct tgccattggt gccggtgatg ccgatcgtcg gcacggttcg
    12961 cttcgaatcc ggccacatct cgggcagatc gctggcgtcg ggctctgctg tcaggagggc
    13021 acggatctca ccgagctcag cctccaagtc agctgcctca ccggcgacca ggcgccacgc
    13081 caggtgccca agcaccttgc tggcgcgacg gcgatcccag gagaaggcga cggccgcatg
    13141 cccctcttcc tcgagctcgc gcaccacgat ctccgccaga cccatgtcga gctcggcgtg
    13201 cagccaggca accacccccg cgagctgctt cgcgatgtcg atccggccgc tcgcgtcttg
    13261 gggaacatcg acgcgctcgc gcaccaggcc aagaatctcg gggtcgagcg ccgaccgctc
    13321 ctcgtcgctg gcgcgcaact ccagcttgat cgcggggcgc agcaggaaca ggttcgggcc
    13381 atcgagcgta cggagttcta cggcatccac gacttctctc ccgttccgtt caggtggtac
    13441 gccgctaacc gacgccctgc agggcgacaa cagcggcgtt gtagagcgca tgcgcgatga
    13501 tcgctggtgt ggtgctcata tacttgcgaa tcagtccgaa caggatacca gcgaggaaga
    13561 gcccgagcac aacaaaggtc aactggtact gcgtgtgcac cagcgcccag agcgcggcgg
    13621 tgatcacgat gccgaagcgt ggctgaatcg cgccacggaa gaggatctcc tcaccgatgc
    13681 cggccgacgc gaagatcagc aggctgacga tcgtgaagtc cgtgccggat gccatctcct
    13741 ccatcgtttc gccgagatcc tcgaagcggt ccggctggaa ccagagcgtc agcatcgagc
    13801 cgagaatgct cagaccaaag gtggggacca ccgccgcggc cccaaccgcg aagtcctgca
    13861 gcgtgggacg cgtgaagccg agccgagcag tcacttcgtc aacgttgcgg taatttcccg
    13921 tgccgacggc gacataggca accgccacga agaacaggac gttgatgagc tgcgccccga
    13981 cggttacccc ctcgatcggc tcatcggccg ggccgagtga catcgcatag ctgaagtagg
    14041 cgaacagcgc gaagatgaca ccgagtccca tcatgtcgat gggggatgcc gggtccaacg
    14101 gcgtcacata cgagaggagt ttcctgaagc cacgaatcaa cggcaggctg aatgcgaggc
    14161 cgaacgccag cacgaaccag ccgtcctcgt gaccattcgc gagcagtgcg acgccggtga
    14221 cggtcagcag caggcctggt atgccgtaga gcaggtagag gcccacgttc gccgatcgat
    14281 ctgtctccgc ctttcgcgcc cacagcagga ccccgtagac ggcggcgagg atgatcaaca
    14341 gaacgctgag ggtcagcagg aagtccatga acaggcgtat ctccggaggg caacagtgag
    14401 ggcacccgga tcgggcacaa ccgtggcaag tatacggctc acctgcgacc gtccggtgcc
    14461 aagggcgatg ctagacttgg gcagtcttga gcaccggctc ccggagcaag ttgggagcgc
    14521 agcgcaccgt cctcggagta ccgatccgac catggcagca gaccgccggg aaaccggcaa
    14581 acgccagcaa cgtgcccgca ggggcggcct cggcaagggg ctcgacgccc tgattcctgc
    14641 ccgccaggag ccggaggtgg ctcgaccaga tggttccggc gcagcggaac agcgctccgg
    14701 ctcgacggag ttgccgatcg acagcatcgc cccaaacccg tggcaaccac gctcacacct
    14761 cgaccaccag caccttgagg aactgacggc ctcgatccgc actcacggca tcgtgcagcc
    14821 gctcatcatc actccagctg acgagccgga ccgctaccac cttatcgccg gtgagcgtcg
    14881 ctggcgagca gcgcaacgcg ctgggctaac ctccgtgccg gcgatcatca agtccgctac
    14941 gccccaggcg atgcttgagc tggcgctcgt cgagaacatc gttcgcgccg acctcaaccc
    15001 gattgaggaa gcggaggcct tccggcaatt gatcgaggaa ttcggcatgt cgcaagccga
    15061 actcgctggc cgcgttggcc gctcccgggt ggcggtaacg aacacgctgc gcctgctgaa
    15121 cgcaccagat gccatccggg aagcgctgct gcgcggccac gtcaccgagg gccacgcgcg
    15181 ggcgctgctg ggactgcccg gggcggcgga ccagaccgct gccctgcaga cggtgatcga
    15241 gcagcagatg agcgttcggc agacggagca gctggtccgt cgctggaaca cgagcgaccg
    15301 caagccacag gagccgcgcg agcgcgaccc cgaggagcgc cgtgtcgagg aggagttccg
    15361 gcaggcgttg gggacgaacg tcgccttccg ccggcggaca ggcggcaacg gtggcacgct
    15421 gacgatccag gtctactccg acgaggagat gaacgcgctc tacagccgcc tcatcggcga
    15481 cgacagttgg taacggtcca catagagacg ggccgtgtac cggctcattt gtcgtgttgc
    15541 ggcacttgca gacccgcact ttgatgcgcc ggttcgccat gaggaagacc agtagccgag
    15601 aactcaggac tgcaaagcgg ctgtccgcct gagcgcaggc cggagaaggt ggctgttgcc
    15661 aaccctgatg cggggctcag cagctgagat cgtcccgaat ctccgtttcg gttgcgcccg
    15721 tctgtgcgtc gatgcggacc acgatatcgt tcgtcgtgcg ctcgcgctcg tcgtagtaca
    15781 cgaccaccca atgcggctcc tcgcggtccg ttgtgtccag cacgacctgg acatggcgcc
    15841 gggatagctc gcaccccgat cggtactcct cccccaccac ctcctgcgcc acgcggaatg
    15901 cctgctccgc cgagatcggc agatcagcta tcaggttccc gatcggctcc ggtggctcta
    15961 ccgtcgaggt gatctcgtcc tcgtggtaga tcacaccgct ttgcctccct accgccaagg
    16021 tcagccgtgg ccactccccg tcctcctttg gcgcagcgaa ggtaacgagc accgtgccct
    16081 cgtcggcaac gggagtcgca ttcccggggt ccggcgggta ggagaactgg gccgacacga
    16141 ggatcggcca cgcctcgtcg tcccactcct ccgcccaatc gctggcgatg tcaacggtgt
    16201 cgcggaccgg taccggtcgc ggcacctctg cgtcgaccgc cggtcgctct tcgaccggtc
    16261 cgatgaccgt tgtcggctcg gtacctaacc gcccaacgac aatgaccgac acgacgatca
    16321 cgacgatgaa gaacagcgcc acgagcgtcc gctcggcggc cgtcagcgtc gattccgttg
    16381 tctgctcggt catccagcgc ctcaaggggt caacaatcct ggtatcgctc cggccagtgt
    16441 cgcagagggt gtcgccccag cgccaaccgg cgacaggcct cgctacagcg gcatcgtata
    16501 ctccaccgtg gcgttcatcg ccaagctgac cgacaccacc cgaactctgt ttcaggag
//
LOCUS       DASYWL010000306         1909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_447410, whole genome shotgun sequence.
ACCESSION   DASYWL010000306 DASYWL010000000
VERSION     DASYWL010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447410"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="VGR22_10855"
     CDS             <1..824
                     /locus_tag="VGR22_10855"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEV2129107.1"
                     /translation="EEISQPAITAQIKALERYFGTQLLERGGRGARLTPSGQLVADYG
                     QRVLALVDELGRAVADLEQLAAGQLVIGASSTVGEQLLPAYLGRFHHQYPGVALEVRI
                     GNTDEIIERVLHREIDLAFVGRTPSNDNLTAEPVFADDIVAFVAPGDPRLASAPLSPS
                     ALNGQQFVMREEGSATRELAERCLRSAGCSPGHIVELGSNEAVKRAVEAGLGVGLLST
                     HAIEAERLAELLVDLPLSPWSCHRSFWLIARRDRLLTRAEQAFLALIRAGGTRPG"
     gene            1375..>1909
                     /locus_tag="VGR22_10860"
     CDS             1375..>1909
                     /locus_tag="VGR22_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129108.1"
                     /translation="MLEIGNRYRRRDLHRHYGGQWQGGISTPSAHPLVFLFTGETGEQ
                     YGYRDGWEPDGTFRYTGEGQLGDMAFVRGNAAIRDHAAEGKDLHLFEVSGRGFVRYVG
                     QMVCAGYDIVPGIPDWEGSLRAAIVFRLVEADAVASAGQEEDAAVPGTTSTDASGSLW
                     TRPLDEVRALALRPPPAS"
BASE COUNT          336 a          640 c          601 g          332 t
ORIGIN      
        1 ccgaagagat tagccagccg gcgatcaccg cccagatcaa ggcccttgag cgctacttcg
       61 gcacgcaact attggagcgc ggcgggcgcg gcgcccgctt aacaccctcg gggcaactgg
      121 tcgccgacta tggccagcgc gtcttggcgc tcgtcgacga gctcggccgg gcagttgccg
      181 acctggagca gttagccgcg ggacaactcg tgattggggc cagcagcacc gtcggggagc
      241 agttgctccc agcgtatctt ggtcgcttcc accaccaata cccaggcgtg gcgctcgagg
      301 tcagaattgg gaatacggac gagatcatcg agcgcgtgct gcaccgcgag attgaccttg
      361 cattcgtagg caggacgcca tcgaacgaca acctcaccgc cgagccggtg ttcgcggacg
      421 acatcgtggc atttgtggca cccggcgatc ctcgcctggc aagcgcgccg ctgtccccgt
      481 cggcgcttaa tgggcagcag ttcgtcatga gagaggaggg ctctgccacg cgcgagctcg
      541 cggagcgatg tctccgttcc gccggctgct cgcccgggca catcgttgaa ttggggagca
      601 atgaggcagt caagcgagcc gtcgaagcgg gcctaggcgt tggtttgctc tccactcacg
      661 cgatcgaagc agagcgactt gcggagctcc tggtcgacct accgttgagc ccgtggtcat
      721 gccaccgctc gttttggctc atagcccgtc gcgacagact gctcacacgc gccgaacagg
      781 ccttcctggc cctgatccga gcgggcggca ctcgtcccgg ctgatggtcg ctgctccagg
      841 cgatggcaat gcgaccacgt cgcactacaa atccccgagc ggcaagcaga ttcgcccatg
      901 caaggtccta tctccatcta tagagctctc gttcaccgaa tgggagcgcg gtatcgccat
      961 gcgggggcag ggcgatattt tcagcgatgt agagggatgc gcctcctcaa tgcggaaatg
     1021 ccatgccgcc gttgctggtt cgcatatgaa gcgcgccgca gatcaccgcg cacctcgacg
     1081 agtgggcgcg cacaatccgc cccaccgtcc cgggtccagt cctgcagctg ggcggccagc
     1141 tccattcgcc gggcagaacc tccacgatct ccaccagcag cgtgtcgccc ggctccgctc
     1201 ccgcaatgaa gaccggtccg gtaagggcgt gccccccatg cctcggtgcc cggccccgtg
     1261 gcaggctcat gacgtgctgc ggcagcggtt ccacatccac cggaccaatt cccatccaaa
     1321 tgccgccaaa ccaagcgagg cgtgatccga ggcgaattat cccgaggtgg acgcatgtta
     1381 gagatcggta atcgataccg tcgtcgcgac ctgcaccgac actacggcgg tcagtggcag
     1441 ggcgggatca gcactccgtc tgcccacccg ctcgtcttcc tcttcaccgg cgagacaggc
     1501 gagcagtacg gctaccgtga cggctgggaa cccgacggca ccttccgcta taccggagag
     1561 ggtcagctcg gcgacatggc ttttgtgcgg ggcaacgccg cgatccgtga ccacgcggca
     1621 gagggcaagg acctgcacct ctttgaggta tcgggcaggg gtttcgtccg ctacgtcgga
     1681 cagatggtct gtgccggtta cgatattgtt ccaggtatcc ccgattggga gggaagcctc
     1741 cgcgcggcca ttgtcttccg actggtcgag gctgacgccg tcgcttctgc cggccaggag
     1801 gaggacgcgg ctgtccccgg cacaacgagc accgacgctt ccggctcctt atggactcgg
     1861 ccactcgacg aggtgcgggc cctggcgctc cgacccccac ccgctagcc
//
LOCUS       DASYWL010000307         9384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_90697, whole genome shotgun sequence.
ACCESSION   DASYWL010000307 DASYWL010000000
VERSION     DASYWL010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9384
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90697"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(257..1255)
                     /locus_tag="VGR22_10865"
     CDS             complement(257..1255)
                     /locus_tag="VGR22_10865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000970946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEV2129109.1"
                     /translation="MQTTAVHRSYETDRGIAYCGDSLNLLDNLAPESVDLILTSPPFP
                     LLRKKPYGNEEQVDYVDWLMRFGQRALPVLKSTGSFVLDLGGAYQKGKPVRSLYQYHV
                     LIRFVEELGYHLAEEFFWHNPSKLPSPTEWVNKRKIRAKDTVNTVWWFSKTEWPQANV
                     RNVRTEYSERMKKLLKDSSKYYTPKERPSGHDISERFGKNNGGALPSNMLSIPNTESN
                     STYLRYCRSVGAVEHPAKFPAKLPEFFVRFLTGPGDVVLDIFAGSNTTGEVAESLQRR
                     WIACYLDQAYVAASALRFVSSEEGANDVYQRIMAGEAVSIESPYSASIDEQAKVVD"
     gene            2046..2987
                     /locus_tag="VGR22_10870"
     CDS             2046..2987
                     /locus_tag="VGR22_10870"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEV2129110.1"
                     /translation="MNWWILVIALLAVAALLVKVFDRKAPGTLYKTALLVWAIGPLVG
                     TVWAMVLLWQEWISWTDLALFLGFYVATGMGITLGFHRLLTHSSFETPAPVRAIFLIL
                     GSMAGQGRPIDWASNHLKHHAFSDQEGDPHSPVEGLFHAHLGWFFTGEPADRARYGRK
                     FEQDRVIAFVDRTTEVWLVLGLVIPFLIGGWSGFLWGGLVRMAFLNHATFAVNSICHA
                     FGARPFDTKDESRNNWIIAMLTLGEGWHNNHHAFPAMAYHGMSWRQFDPTAVMIRLLV
                     WLRLAKNVKLPRPELVDRRLTKPAEAPATVQPTTTGD"
     gene            complement(3147..5117)
                     /locus_tag="VGR22_10875"
     CDS             complement(3147..5117)
                     /locus_tag="VGR22_10875"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HEV2129111.1"
                     /translation="MPDPTTDRIPYATPVDVRFSPNTAHRPVPIGSVILDDQIWAPRI
                     WLNRQRTIPAQYTLLEETGRLNNFRRVAGPYESGHVGREFNDTDVYKWIEAASWALVH
                     ASDQERAEIEGLIDAAIEIVAAAQQDDGYLNTYFALEREGQRWSNLRDLHELYCAGHL
                     IQAAVAHARVTRKTRLLEVATRFADLICATFGSASEGKREETDGHPEIEVALIELYRT
                     TGETRYLDQARFFIDVRGRGTIGGREYHQDATPLRQQTEMVGHAVRAVYLNAGAADLL
                     AEERDPALRSALDAMWENMTTRRSYISGGVGSRWEGEAFGEDYELPNTRAYAESCAAI
                     GSVMWAWRMMLLVDEDDTRWVDWIERTLLNAMLPGISLDGEQYFYQNPLEDDGDHRRQ
                     PWFGVACCPPNIARMLGMVPGMVATVTTRRAVGEELGPLHDTVWLHLLAQGTVQVELP
                     GGGSVTLAMRSRYPWDGDIEIEVTELVDAGTFMLNVRVPGWCDGASGEVNGEALVGAE
                     TAPGQYATLNRTWQVGDTVRLRLPLRIRRMEAHPRVTEDAGRAALMRGPLLYCVEAAD
                     NERGDVRDLVLGSARDLHPGWRPDVLGGCVVVSGTAELEPVDAGWRDRLYREIGPTAP
                     PAPRETVTLQAIPYHLWANRGRGPMTVWLRRE"
     gene            5289..5867
                     /locus_tag="VGR22_10880"
     CDS             5289..5867
                     /locus_tag="VGR22_10880"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HEV2129112.1"
                     /translation="MSTEKQNHKVIADWIGDIVALEGHIEEALDHQIKLKSDSPQLTS
                     TIQRFHDTVRDSKRRAVSYEKEYGTEASKGIIERGSELLGKAAGIIDRLRADSISKAL
                     RDDFTAFNHAAIAYSTLHATSMALGDSATEAFSGEGLRTYAALSQDVNQVISEAVVRD
                     LKSNPDYGHVDTAVAEQCRQFINQTWRATSDT"
     gene            6109..6381
                     /locus_tag="VGR22_10885"
     CDS             6109..6381
                     /locus_tag="VGR22_10885"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEV2129113.1"
                     /translation="MEFEWDEAKRLSNIDKHWIDFLDARRLFDGRPVFRTISAYRGEE
                     RYLTVGFLGGQFVTAVWTRRNGTIRFISVRHARRNEIASILATHEY"
     gene            6485..6757
                     /locus_tag="VGR22_10890"
     CDS             6485..6757
                     /locus_tag="VGR22_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129114.1"
                     /translation="MSNKNHEERIVRYTAEEIDEMLARGESKTDWERVRNMTEEELEA
                     SIDVEEEGEFDWDTVYVGMPPVPAKKRVTLWYDFDVIEWFQNQGPD"
     gene            6930..8141
                     /locus_tag="VGR22_10895"
     CDS             6930..8141
                     /locus_tag="VGR22_10895"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2129115.1"
                     /translation="MSATSRANGSPIRGIRKTQAVLLALVVLAFNLRAPILAVPPVLE
                     TIQRDLGLSGVAAGLITTLPVLCFGLVSPLAPVLARRLGLDTTLMLAMTAIATGVIVR
                     MGQPALALYLGTLGLGTGIALLNVLIPAFVKREAPNRVGTMTSLYTMLLNAGAGVGSA
                     LTVPVMAATGWGWRPALGIWAIPAAIGIAVMVPWVLHQRRGAAGLRTLTRRRGLWNSP
                     LAWQVTIAMGTQSMIFFSVAAWLPTLLQDAGMGEARSGAMLSVMTLSGLGGSFITPIL
                     ANRTRSQSWLSPVTTTLLAGPLVALIVAPLWGTVLWVAMLGFGLGMIIALMLTLIILR
                     APDAQRASDLGGMAQGVGYLFASMGPIILGAIHDRTDGWTLPLIFCVALLVPYSINAW
                     LAGRPRLVSAH"
     gene            complement(8087..8419)
                     /locus_tag="VGR22_10900"
     CDS             complement(8087..8419)
                     /locus_tag="VGR22_10900"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEV2129116.1"
                     /translation="MQQANPITCGSCGAENPPDAEYCENCGKPLTRSAQEGSAEQGET
                     QVGGGILGIGEGDADVDAPGLEEGPRRRGTRTGVADTSGECPGTIAPPDQCAETSRGR
                     PASQALME"
     gene            8577..9209
                     /locus_tag="VGR22_10905"
     CDS             8577..9209
                     /locus_tag="VGR22_10905"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2129117.1"
                     /translation="MGTPTARHDKTTRFASWQSIFAATYQDKLAGRFPTMKAALNLFL
                     QHDGTTIVETGTLRWVESLEHGWEGDGNATYIFGEVLQEFGGHLWTCDADPVAIAESR
                     EGTKRFADHITYVNQDSVAFLDSFDHPIDLLYLDSMDCPREGDASAAQAHNLRELQAA
                     LPKLSRNGVTLIDDNGYPNGGKALKSKQHLAEQGWLCLYDAQQTLWMRRC"
BASE COUNT         1638 a         3090 c         2957 g         1699 t
ORIGIN      
        1 gtttctcacc gtactggcgc tgaacgtcct tggcgatggc atgcgtgatg cctttgaccc
       61 ccgctcccat cgccgctagc ggcgaccaca ccccggtcat cctgagcgca gtcgaagagc
      121 ctgtcctgag cgtagccgaa ggaattcccc ggccctgcca atttgttctc gtctgtgcgt
      181 ccacggagca gtttacggaa gaatcccacg tcgcctccaa atccattgat gctatacaac
      241 ggcgagcgag ctcaattcag tcaaccacct tggcctgctc atctatagac gctgaatagg
      301 gcgattctat tgatacagcc tcgccagcca tgattcgctg gtatacgtca tttgcccctt
      361 cctctgaact gacgaagcga agggccgaag cagcgacata ggcttgatcc aaatagcaag
      421 cgatccacct gcgctgaagg ctctccgcca cttcgccggt ggtgttcgag ccagcgaaga
      481 tatccagcac tacatcgccc ggcccggtaa ggaaacgaac aaagaactcg ggcagtttgg
      541 ctggaaactt ggcgggatgc tcaactgctc cgacgctcct gcagtagcgg agatacgtcg
      601 agttgctctc cgtattcgga atagaaagca tgtttgacgg aagcgcgccc ccgttatttt
      661 tgccgaatct ttcggagata tcgtgaccag agggtcgctc tttgggcgtg taatacttgc
      721 tgctatcttt cagcagcttt ttcatgcgct cggaatactc tgtgcggaca tttcgcacat
      781 tcgcttgcgg ccactcggtc ttcgagaacc accatactgt gttgactgtg tccttggccc
      841 tgatcttccg tttatttacc cattccgtcg gagacggtag tttagaggga ttgtgccaaa
      901 agaactcttc cgcgagatgg tagcccaact cttccacgaa tcggattaac acatggtact
      961 gatagaggga acgtaccggt ttacccttct gataagcacc acccaaatcg agcacaaaag
     1021 aaccggtcga tttaagcacc ggtagtgcac gctggccaaa ccgcatcaac cagtccacat
     1081 aatctacttg ttcttcgttc ccatatggtt tcttccgtag aagtgggaac gggggagagg
     1141 tgaggatcaa atcgactgat tcaggggcga gattatcgag cagattcagg gagtcaccgc
     1201 agtaggcgat gccacgatcg gtttcataac tccgatgcac agccgttgtc tgcacgacca
     1261 ctcccacttg ctaattatcc ctgcattgac gttttccgag tatacgccat cgcatgactt
     1321 gcagcagcaa tcacagcttt gccaattgct cgtcgatgat gtcgctggat cgccggtggg
     1381 attgcaatat ggatgaatag ggtgccgacg tgcctaaggc aggattctag tagcacgtct
     1441 cccagcggct agcgagcaga ccttgcacgt ttggaagtcg aggctaccaa ccagtccctg
     1501 tatccggaat tctggcagtg tggccgtctt cgaaggttgc cagaatgggc ggggatttct
     1561 ggcagggttg gtttgcccgc cgtttcgccg aaatcggaag gctgccacac ggccacatat
     1621 gtcgatttct ggcagccttg atcgtcgtag gggaggagct tgtctcctcc ctttggcgtg
     1681 ggcagatatt gaggaaggga gcacacgagg tgctccccta cgggttggtg ggttcgcggc
     1741 tcacccgtac gagtggggcc aggcggcgtg gttactcccg gtgcagccag acggccatcg
     1801 ggccgcggcc ggggttcgcc cggaggtggt gcggggccgc ctggacggtg actggctccc
     1861 tacctcacga ccgctccggt agcgctcgat ggtgcccggc acggcgggcg ggtacacttc
     1921 ctcctggttg tgacacccca tgaaccggtg aatcgactca ccattgaggt ggagtttctt
     1981 gatcctggct ccgcgctcga catgcccaag gggcgcaggg atcacaggat caggagacac
     2041 cccgcatgaa ttggtggatt cttgtcatcg cgctgctcgc cgtcgcggcg ctgctcgtca
     2101 aggtgttcga ccggaaggct ccgggcacgc tctacaagac agccctgctc gtctgggcga
     2161 tcggcccgct ggtcggcacg gtctgggcca tggtcctcct ctggcaggag tggatcagct
     2221 ggaccgacct cgccctcttt ctcggcttct acgtcgcgac cgggatgggc atcacccttg
     2281 gcttccaccg gctgctgacc cacagcagct tcgaaacccc ggcgccggtg cgggcgatct
     2341 tcctcattct cggcagcatg gccgggcagg gccggccgat cgactgggcc tccaaccatc
     2401 tcaagcacca cgccttctcc gaccaggaag gcgatccgca cagcccggtc gaaggcctct
     2461 ttcacgcgca tctcggctgg ttcttcaccg gcgagccggc cgaccgcgca cgctacggtc
     2521 ggaagttcga gcaggaccgt gtgatcgcct tcgtcgatcg caccaccgag gtctggctgg
     2581 tgctcggcct ggtcatcccg ttcctcatcg gcggctggtc gggcttcctc tggggcggtc
     2641 tagtccggat ggccttcctc aaccacgcca ccttcgccgt caactcgatc tgccatgcct
     2701 tcggcgcccg tccgttcgac accaaagacg agagccgcaa caactggatc atcgccatgc
     2761 tgaccctggg cgagggctgg cacaacaacc accacgcctt cccggccatg gcctaccacg
     2821 gcatgagctg gcgccagttc gacccgaccg ccgtcatgat ccggctgctc gtgtggctgc
     2881 gactggcgaa gaatgtcaag ctgccgcgcc cggagctggt cgaccgtcgc ctcaccaagc
     2941 cggcggaagc cccggcgacc gttcagccca cgaccaccgg cgactaggcg cacccgccaa
     3001 ggcgtttgtt tgcaccatcg gccaccacac gatgaagggt agcggacctg ccccgccctt
     3061 catccggcac tccggggtcc gtgggccatc catcggcaac ccggaggagc cgcagaaccg
     3121 acccggctgc ggacgggagc gatccactac tcccggcgca gccagaccgt catggggccg
     3181 cgcccgcggt tcgcccagag gtggtacggg atcgcctgca gcgtcaccgt ctcccgcgga
     3241 gcaggcggcg cggtcgggcc gatctcccgg tagagcctgt ccctccagcc ggcatcgacc
     3301 ggctccagct cggcggtgcc ggagaccacc acgcagccgc ccagcacatc cggccgccag
     3361 ccagggtgaa gatcccgcgc cgagcccagc acgaggtcgc ggacatcgcc gcgctcgttg
     3421 tcagccgcct ccacgcaata gagcagcggc ccacgcatga gcgccgcccg gccggcatcc
     3481 tccgtgacgc gcgggtgggc ctccatccgc ctgatgcgca gcggcagccg cagccgcacg
     3541 gtgtccccca cctgccacgt tcggttcagg gtggcgtact gtcccggcgc ggtctcggcc
     3601 ccgacgagcg cctcgccgtt gacctcgccg ctggcgccgt cgcaccagcc gggcacgcgc
     3661 acgttgagca tgaaggtgcc agcgtcgacc aactccgtca cctcgatctc gatgtcgccg
     3721 tcccatggat agcgggaacg catcgccagc gtcaccgacc cgccgccggg cagttcgacc
     3781 tgaacggtgc cctgcgccag caggtgcagc cagaccgtgt cgtgcagcgg ccccagctcc
     3841 tcgccaacag cccggcgcgt ggtcacggtg gcgaccatcc ccggcaccat gccgagcatg
     3901 cgggcgatgt tcggcgggca gcaggcgaca ccgaaccacg gctgccgccg gtggtcaccg
     3961 tcgtcctcca gcggattctg gtagaagtac tgctcgccgt cgagcgagat gccgggcagc
     4021 atcgcgttga gcagcgtgcg ctcgatccag tcgacccagc gggtgtcgtc ctcgtcgacg
     4081 agcagcatca tccgccacgc ccacatcaca ctgccgatcg ccgcgcatga ctcggcgtag
     4141 gcgcgggtgt tgggcagctc gtaatcctcg ccgaacgcct cgccctccca gcgggagccg
     4201 acgccgccgc tgatgtagct gcggcgggtc gtcatgttct cccacatcgc gtcgagcgcc
     4261 gagcgcagcg ccgggtcgcg ctcctcggcg agcaggtcgg cggccccagc gttgaggtag
     4321 accgcgcgga ctgcgtgccc gaccatctcc gtctgttgcc gcagcggggt ggcgtcctgg
     4381 tggtactccc tgcccccgat ggtgccgcgc ccgcggacgt cgatgaagaa gcgcgcctgg
     4441 tcgaggtagc gcgtctcgcc ggtggtccgg tagagctcga tcagcgccac ctcgatctcc
     4501 gggtggccgt cggtctcctc gcgcttgccc tcgctggcgg acccgaaggt cgcgcagatg
     4561 aggtcggcga aacgggtcgc gacctccagc aggcgcgtct tgcgggtcac ccgtgcatgg
     4621 gcgactgcag cctggatgag gtgcccggcg cagtagagct cgtggagatc gcgcaggttg
     4681 ctccagcgct ggccctcgcg ctccagtgcg aaatatgtgt tcaggtagcc gtcgtcctgc
     4741 tgcgccgcgg cgacgatctc gatcgcggcg tcgatcagcc cctcgatctc cgcccgttcc
     4801 tgatcggacg cgtgcaccag cgcccagctc gcggcctcga tccacttgta gacgtcggtg
     4861 tcgttgaact cgcgtccgac gtgcccactc tcgtatgggc ctgcgacgcg ccgaaaattg
     4921 ttgaggcgtc cggtctcctc gagcagtgtg tactgtgcag ggatggtgcg ctggcggttg
     4981 agccagatgc gtggcgccca gatctggtcg tcgaggataa cgctgccgat cggcaccggt
     5041 cggtgcgcgg tattcgggct gaatcggaca tcgacgggtg tcgcgtaggg aatgcggtcg
     5101 gtggtcgggt ctggcatcgg tgctgctccg gggatcggtg cggatgtgcc ggcgttctgg
     5161 cgccattgtc ccagatcgtg gctgttgcac gagcagggaa ccggcgtggc agggattctg
     5221 cgtgaccttc gcgtagcggg gcgccaccct agcgccgttt ggataatcgc gcaagcaaag
     5281 gagcacgcat gagcaccgag aagcaaaacc acaaggtcat cgctgactgg atcggtgaca
     5341 ttgtcgcgct ggaggggcat atcgaggagg cgctcgacca ccagatcaag ctgaagagcg
     5401 acagccccca attgacgtcg acgattcagc gattccatga caccgtgcgg gacagcaaac
     5461 gccgcgccgt atcgtatgag aaggagtacg gcacagaggc cagcaagggg atcatcgagc
     5521 ggggctccga gctgctcggc aaggcggccg ggatcatcga ccggttgcgc gccgacagca
     5581 tctccaaggc gctgcgcgac gatttcaccg ccttcaacca tgcagcgata gcctactcga
     5641 cgctgcatgc cacatcgatg gcattgggcg acagcgcgac cgaggcgttc tccggcgagg
     5701 ggctgcgcac ctatgccgca ctatcacagg acgtcaacca ggtgatctcc gaggcggtcg
     5761 tgcgcgatct caagtcgaac ccggactacg ggcacgtcga cacggcggtg gccgagcagt
     5821 gccgccagtt catcaaccag acctggaggg ccaccagcga cacgtagtcg ccgctcgaca
     5881 gcacgagacg acaacggcag cccggccggt gggagcgttc ccggccgccg ggtttgtcgt
     5941 gccgcgggga gctgatcgca accgtgtcgc cccctctcca tctgggcggg gccggtgcgc
     6001 cgacagatgg ttggttcccg gagggccggg ctacctgttc cggccacctc atgcgaacct
     6061 gagcacatcg gccgagtcgt cgacggcggg tgcgagggag aagcgctggt ggagttcgag
     6121 tgggacgagg cgaagcggct gagcaacatc gacaaacatt ggatcgactt ccttgatgcc
     6181 aggcgtctgt tcgacggaag accggtgttt cgaacaatca gtgcctatcg aggcgaagag
     6241 cgctacctca cggtgggctt tctcggtggc caattcgtga cagcggtctg gaccaggcgg
     6301 aacggcacga ttcgcttcat ttcggtaagg catgcccgca ggaatgagat cgcctccatt
     6361 ctcgccacac acgagtattg atggctcata ccgacacgat ttctggcgct cgccgtactt
     6421 gacagaatgc actccgtgtt ttgctacact cgtagctacg ttgcagagaa cgtgaggcga
     6481 tgatatgagc aacaagaacc acgaagagcg catcgtccgc tacaccgctg aagagatcga
     6541 cgagatgctc gcccgcggcg aaagcaagac tgactgggaa cgggtcagga acatgaccga
     6601 ggaggagctt gaggcctcga tcgacgtcga ggaggagggc gagttcgact gggataccgt
     6661 ctacgtcggt atgcccccgg tcccagccaa gaaacgagtc accctctggt acgacttcga
     6721 tgtgatcgaa tggttccaga atcaggggcc tgactaacga gacccgcatg aaccaggtcc
     6781 tgcgcgccta cgtcgaggcg cagcgcaaca agaaagcctc ctgaccccac agcccatact
     6841 cccgatccct gcggtaaagt gaagccacca cccgtccacg cggcgggtgg tcgtcatgtc
     6901 tgtcgatcac ccaccggaac cctgcaccca tgtccgccac cagccgggcg aacggctcgc
     6961 ccatccgcgg catcagaaaa acccaagcgg tgctgctggc actcgtcgtc ctcgccttca
     7021 acctgcgcgc gccgatcctg gccgtgccac ccgtgctgga gacaatccag cgcgatctcg
     7081 gcctctccgg cgtcgccgcc gggctgatca ccactctgcc cgtgctctgc ttcgggctcg
     7141 tctcgccgct ggcaccggtg ctcgcccgca ggctcggcct cgataccacg ctgatgctcg
     7201 cgatgaccgc gatcgcgacc ggcgtgatcg tccgcatggg gcagccggcg ctcgcactct
     7261 acctcggcac gctcgggctc ggcaccggca tcgcgctgct gaacgtgctg atccccgcct
     7321 tcgtcaagcg cgaggcgccg aacagggtcg ggacgatgac ctcgctctac acgatgctgc
     7381 tcaacgccgg tgcgggcgtc ggctcggcgc tgacggtgcc ggtgatggcg gcgaccggat
     7441 ggggctggcg accggccctc ggcatttggg cgatcccagc cgcgatcggc atcgcggtga
     7501 tggtgccatg ggtgctgcac cagcgacgcg gggctgccgg gctccggacg ctgacccgcc
     7561 gccggggcct ctggaacagc ccgctggcat ggcaggtgac catcgcgatg ggcacgcaat
     7621 cgatgatctt cttctcagtg gccgcgtggc tgccgacgct gctgcaggat gcgggcatgg
     7681 gcgaggcgcg ctccggggcg atgctctcgg tgatgacgct ctccggcctc gggggctcgt
     7741 tcatcacgcc gatcctcgcc aaccggaccc gctcccagag ctggctgagc ccggtgacga
     7801 cgacgctgct ggccgggccg ctggtggcac tcatcgtggc accgctgtgg gggacggtgt
     7861 tgtgggtggc gatgctcggc ttcgggttgg ggatgatcat cgcgctgatg ctgacgctca
     7921 tcatcctccg cgcgccggac gcgcaacggg catcggacct gggcggaatg gcgcagggcg
     7981 tcggctacct cttcgcgagc atgggcccga tcatcctcgg cgcgatccac gaccgcaccg
     8041 acggctggac actgccgctg atcttctgcg tggcgctgct ggtcccctac tccatcaacg
     8101 cctggctcgc cggtcgcccg cggctcgttt cagcgcactg atcaggtgga gcgatcgtgc
     8161 cggggcactc gccgctggta tcagccaccc ctgtgcgggt tccgcggcga cggggcccct
     8221 cttcgagacc aggcgcgtcg acatcggcat cgccctcacc aatgcccagg atcccgccgc
     8281 ccacttgcgt ctccccctgc tcggccgacc cttcctgggc cgaacgggtc agcggcttgc
     8341 cacagttctc gcagtactcg gcatccggcg ggttttcggc tccacaactg ccacaggtga
     8401 tcgggttcgc ttgctgcatg gttgcctcct gttcgtgtca ccaccgcctc ggctggcgat
     8461 gcggtgcccc gtacgcgcaa gtcacaggcc atccaacact ggaagccatc ttgcaggtgc
     8521 atacccacta ccgcgcgccg cccactggcg aatatcaacg aaccaaggag gcgatcatgg
     8581 ggacaccgac agcgcgacac gacaagacca cgcgcttcgc atcctggcag agcatatttg
     8641 cggcaacgta tcaggacaag ctggccgggc gctttccgac gatgaaggcc gcgctcaacc
     8701 tgtttctcca gcacgacggt accaccatcg tcgagaccgg cacgctgcgc tgggtcgaaa
     8761 gcctggagca tggctgggag ggtgacggta acgcaaccta catcttcggc gaggtgctgc
     8821 aggagttcgg cggccacctg tggacgtgcg atgccgatcc ggtggcgatt gcggagtcgc
     8881 gcgaaggcac gaagcggttc gccgaccaca tcacttacgt gaatcaggat tcggtcgcct
     8941 tcctggacag cttcgatcac ccgatcgacc tgctgtactt ggacagcatg gactgtccca
     9001 gggaggggga cgcctcggcc gcacaggcgc acaacctgcg cgagctgcag gcggcgctgc
     9061 cgaagctgag ccggaacggg gttaccctga tcgacgacaa tggctacccc aacggcggca
     9121 aggcgttgaa gtccaagcag catctcgcgg agcaggggtg gctgtgtctc tacgacgctc
     9181 agcagaccct gtggatgcgg cgatgctagc cagttcgaga acgaatcgcc gataccccag
     9241 cccctcgtga acgcactctc gctacccgtg tattgttgag gcacccctcg cctattcagg
     9301 gagactggcc tggatattgt gccttcgaga ggctgcggta cactcgtcag gtgatgcgca
     9361 gcgccgatcg atcagcctcc accc
//
LOCUS       DASYWL010000308         1744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_503520, whole genome shotgun sequence.
ACCESSION   DASYWL010000308 DASYWL010000000
VERSION     DASYWL010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503520"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(503..1288)
                     /locus_tag="VGR22_10910"
     CDS             complement(503..1288)
                     /locus_tag="VGR22_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2129118.1"
                     /translation="MDDSQATATATGIHERGEDPAAERPNPLLRVDSIDAAYGKVQIL
                     HDVSMVVHPAEVVSIIGPNGAGKSTVLKSVMGLLNPSAGEVRFAGEAITGMRPDQVVR
                     QGIAFVPQGRIVFKGMTVTENLEMGAYTLGDKRKQRELMNQVFTIFPRLGERPRPQAG
                     TMSGGEQQMLAMGRGMMSEPKMILMDEPSLGLAPLFVEQVFERIRELKAQGMTLLLVE
                     QNAVKSLSISDRAYVLELGRNRFTGTGEALLDDDRVRKLYLGG"
     gene            complement(1278..>1744)
                     /locus_tag="VGR22_10915"
     CDS             complement(1278..>1744)
                     /locus_tag="VGR22_10915"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2129119.1"
                     /translation="MGRTFQLTRLFGRMTVMENLTAISREAQKRAEARALSLLESIGL
                     EGLRDEYAENLSYGQQKLVEFLRLSMIESSLILLDEPFAGVNPVMEQNLIAQLKAWIA
                     EGRTVLLTDHEMAIMMELCQEIYVMDYGEVIAHGIPAEVREDERVMEAYFGR"
BASE COUNT          294 a          627 c          518 g          305 t
ORIGIN      
        1 atggcgggat agcgctcgcc atcatcgccg tggccggtcg gcgcgctcac cggacggccc
       61 ctgccgggga gcgaaacatc gacgatgccg gtgcagccga ggaccgaggt tccatggtcc
      121 tctcagtata gatgcacccc cgccgccaac ggcgcacgcg cacgctcgtg aacccgacag
      181 caggcacgtc gagagcgtgc cccagagttt ccgtagggat cgaccttgtg ttcgcccagg
      241 cctcggaccg acacacggtc ggtccctacg tgtctacggt gacgaagacg gtttcacttc
      301 ctaattgttg caccgtgatc gtcatttcga gccggcgagg cgccatcggc gccaaagagc
      361 ctgcactgag cgaagcgaat gtgtgtcgag aaatcccacg cgaaactgca gacaagagcg
      421 aatcgtgctg ccacttgcgc aagagatttc tccactccgc acgcttcgcg tcctgcggtc
      481 gaaatgacgc cttgggagcc gatcacccgc cgaggtagag tttgcgaacc cgatcgtcgt
      541 cgaggagcgc ctcgccggtg ccggtgaagc ggttgcgccc cagctccagc acataggcgc
      601 gatcggagat cgagagcgac ttgaccgcgt tctgctcgac cagcagcagc gtcatcccct
      661 gcgccttcag ctcccggatc cgctcgaaga cctgctcgac gaacagcggc gcgagcccca
      721 gcgacggctc gtccatcagg atcatcttcg gctcgctcat catcccccgg cccatcgcca
      781 gcatctgctg ctcgccgcca ctcatcgtgc ccgcttgcgg ccgcggacgc tcaccaagcc
      841 gcgggaagat cgtgaacacc tggttcatca gctcccgctg cttgcgcttg tcccccagcg
      901 tgtaggcgcc catctccagg ttctcggtga cggtcatgcc cttgaagacg atccgcccct
      961 gcggcacgaa ggcgatcccc tgccgcacca cctggtccgg ccgcatgccg gtgatcgcct
     1021 cgccggcgaa gcgcacctcc cccgccgacg gattgagcag ccccatcacc gacttgagca
     1081 cggtcgactt gcccgcaccg ttcggcccga tgatgctgac cacctccgcc gggtggacca
     1141 ccatcgagac atcgtgcagg atctgcacct tgccgtaagc cgcgtcgatg ctgtcgacgc
     1201 gcagcaatgg attcggacgc tcggcggcgg ggtcctcccc gcgctcgtgg atgccggtcg
     1261 cggtggcggt tgcttggcta tcgtccaaag taggcctcca tcacacgctc gtcctcgcgc
     1321 acctcggccg ggatgccgtg tgcgatcacc tcgccgtagt ccatgacgta gatctcctgg
     1381 cagagctcca tcatgatcgc catctcgtgg tcggtcagca gcaccgtccg cccctcggcg
     1441 atccacgcct tgagttgggc gatcaggttc tgctccatca ccgggttgac gccggcgaac
     1501 ggctcgtcca gcaggatcag cgacgactcg atcatgctca gccgcaggaa ctcgaccagc
     1561 ttctgctggc cgtaggagag gttctcggca tactcgtcgc gcagcccctc cagcccgatc
     1621 gactccaaca gcgacagcgc gcgcgcctcg gcgcgtttct gggcctcgcg ggagatcgcg
     1681 gtcaggttct ccatcaccgt catccggccg aagagccgcg tcagctggaa ggtccgcccc
     1741 atcc
//
LOCUS       DASYWL010000309         3047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_372313, whole genome shotgun sequence.
ACCESSION   DASYWL010000309 DASYWL010000000
VERSION     DASYWL010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372313"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="VGR22_10920"
     CDS             complement(<1..866)
                     /locus_tag="VGR22_10920"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEV2129120.1"
                     /translation="MNESAPHRDPSANDRTPVVTFGEAMVRLTPPNHERLERTLSLDV
                     TVGGAELNTAVGLVCLGRPASWVSALPGNGLGRMIVRHARTAGVDTSSIHWADERQGR
                     AGVYFLEEGVDPRPSAITYDRKDTAIARVTPGTFNWSSLLSGAAALHVSGITLAISDG
                     ARSEAMEAVRVANAAGIPVAFDLNYRSKLWSEAEAREAFTEIVPKVDILFASRSALRT
                     FFDIDGSHEEVLREALNKLGAAAVTITRKRAKGARRLKIMSMALGQSGVLTTTDWRDI
                     EVVDRLGGGDAYA"
     gene            970..1716
                     /locus_tag="VGR22_10925"
     CDS             970..1716
                     /locus_tag="VGR22_10925"
                     /inference="COORDINATES: protein motif:HMM:NF021788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV2129121.1"
                     /translation="MFAPFDPAHDTDVASAIERLRASLAHTGQLETERVQIPGTRTLV
                     EITRPIDTNRLLDQVVDDPEQNLPYWSEIWPSGIALAGDIIHHPDRVHGKRVIELGTG
                     VGITAAVAIAHGAELTVIDYAEEALTLTRMTCLRHAGAEPADARRVNWRLPDVAALVK
                     HGHFDVVLAADALYERRDIEPLIVAVETLVASDGAVWLAEPGRKPAEVFLSGMAARGW
                     TGPSTQWNGPWPDPKDAGIDVRTHWLQRET"
     gene            1906..3030
                     /gene="ugpC"
                     /locus_tag="VGR22_10930"
     CDS             1906..3030
                     /gene="ugpC"
                     /locus_tag="VGR22_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEV2129122.1"
                     /translation="MSRVIFDNVTKVYGHGEEPAVSNLNLEVQDEEFLVLVGPSGCGK
                     STAMRMVAGLEEISDGRIIIGDRVVNDLPPKDRNVAMVFQSYALYPHMSVRDNLAYPL
                     KLRKVPKDEREERVREAARILDIEAYLDRKPKALSGGQRQRVALGRAIVRNADLFLMD
                     EPLSNLDAKLRVQTRAELVKLHDRVRTTTIYVTHDQTEAMTMGDRIAVLSLGVLQQLA
                     TPQELYDEPANKFVAGFIGSPAMNFLDIKIESNGERLVAVHPGFKMTIPEERVGNLRD
                     YIGKSVTMGIRPEHLVERGMAAGEIPDTALIPATVDVVELLGNEIFVYLDVRGQVITA
                     RMDPSLRLQRGQQIEVVAVPEQLHFFDNETEQTITPFGSR"
BASE COUNT          568 a         1025 c          966 g          488 t
ORIGIN      
        1 gcgtaggcat caccgccgcc gagccggtcc accacctcga tatcccgcca gtcggtcgtc
       61 gtcagcaccc ctgactgccc gagcgccatg ctcatgatct tgagccgccg cgcccccttg
      121 gctcgcttcc gggtgatggt caccgcggcg gcaccgagct tgttcagagc ctcgcgcagc
      181 acctcctcat gagatccatc aatgtcgaaa aaggtcctca gcgcgctgcg ggaggcgaag
      241 aggatatcga ccttcgggac gatctcggtg aaggcttcgc gcgcctcagc ttcactccag
      301 agcttggagc ggtagttgag gtcgaacgcc accggaatgc cggccgcgtt cgccacgcgc
      361 actgcctcca tcgcctccga acgcgcaccg tccgagatcg ccagcgtgat cccggagacg
      421 tgcaacgccg ccgcgccaga gagcagtgag gaccagttga aggtccccgg cgtgaccctg
      481 gcgattgcgg tgtccttgcg atcatacgta atcgccgagg ggcgcggatc gaccccctcc
      541 tccagaaagt agacgcccgc ccggccctgt cgctcgtcgg cccaatggat gctggacgtg
      601 tcgacaccgg cagtgcgcgc atgccggacg atcatgcgac cgaggccgtt gcctggcagc
      661 gcgctcaccc agcttgccgg gcgcccgaga catacgagcc cgaccgccgt gttgagctcg
      721 gcaccgccga cggtcacatc gaggctgagc gtgcgctcga gccgctcgtg gttcggcggc
      781 gtcagccgga ccatcgcctc accaaacgta acgacgggcg tgcgatcgtt ggctgagggg
      841 tcgcgatgcg gcgcggattc gttcatggga acgcctcact cgagtcaaac agcaagaaca
      901 tgtcacactg gaggtactct accgcaggtt ctgccgctgc agaatccgca tgcaccggga
      961 gccgcgccca tgttcgcgcc gttcgatccc gctcacgata ccgacgtcgc ctcggcaatt
     1021 gagcggctgc gcgcgtcact tgcacacacg ggccagctgg aaaccgagcg cgtccagatt
     1081 cccggcacac gcacgctcgt ggagatcacc cggccgatcg acaccaatcg cctgctcgac
     1141 caggtcgtcg atgacccgga gcagaacctg ccctactggt ccgagatctg gccatcaggc
     1201 atcgcgctcg ccggagatat cattcaccac cccgatcgcg tgcatggcaa gcgggtgatc
     1261 gagctcggga ccggcgtggg gatcacagca gcggtcgcca tcgcgcacgg cgccgagctg
     1321 accgtgatcg actacgccga ggaggcactc acgctgaccc gcatgacctg cctccgccat
     1381 gccggcgcgg agccggccga cgcgcgccgg gtcaactggc gattacccga cgtggccgcg
     1441 ctggtcaagc atgggcactt cgatgtcgtg ttggccgccg acgcactcta cgagcggcgc
     1501 gacatcgaac cactcatcgt cgcggtggag acgctggtgg cgtcagacgg tgcggtgtgg
     1561 ctggccgagc cgggacgaaa gccggccgag gtgtttctga gcgggatggc ggcgcggggc
     1621 tggacaggcc cgtctaccca gtggaacggc ccctggccgg accccaagga cgccggaatc
     1681 gacgttcgca cgcactggct gcaaagggag acttgacaat ctcgacgatc cggtgcacaa
     1741 aatgcctagc gaatcataaa gcgccgtggt ttgcggagcg aaagacgccg atggtggcca
     1801 cacctcggga gagcgtggtc atggaatcgt agggcgcttc tggatcacgt tgccggacgt
     1861 accggtcagc ggcgatcgct gtaacatcgg cggaggagta cagatatgtc gcgagttatc
     1921 ttcgacaacg tcaccaaggt ctacggccac ggtgaggaac ctgcggtctc caacctgaac
     1981 ctcgaggtcc aggacgagga gttcctggtg ctcgtcgggc cctccggctg cggcaagtcc
     2041 accgccatgc gcatggtggc aggcctcgag gagatcagcg acggccggat catcattggt
     2101 gatcgcgtcg tgaacgacct tccgccgaag gaccgcaacg tggcgatggt gttccagagc
     2161 tacgcactct acccgcacat gagcgtccgc gacaacctcg cctacccgct caagctgcgc
     2221 aaggtcccca aggacgagcg tgaggagcgc gtccgcgagg ctgcccgcat tctcgacatc
     2281 gaagcgtacc ttgaccgcaa accgaaggcg ctttccggtg gtcagcggca gcgcgtcgcc
     2341 ctcggccgcg cgatcgtccg caacgccgac ctcttcctga tggacgagcc gctctccaac
     2401 ctcgacgcca agctgcgcgt gcaaacacgc gccgagctgg tcaagctgca cgaccgtgtc
     2461 cgcacgacga cgatctacgt gacgcatgac cagaccgagg ccatgacgat gggcgaccgc
     2521 atcgcggtgc tcagccttgg tgtgctccag cagctggcga ccccgcagga gctctacgac
     2581 gagcctgcca acaagttcgt ggctggcttc atcgggtccc cggcgatgaa cttcctcgac
     2641 atcaagatcg agagcaacgg tgagcgcctc gttgcggtcc atcccggctt caagatgacg
     2701 attccggaag agcgcgtggg caacctgcgc gactacatcg gcaagtcggt gacgatgggt
     2761 atccggcctg agcacctcgt cgagcgcgga atggctgccg gggagattcc cgacacggcc
     2821 ctcatcccgg cgacggtgga tgtggtggaa ctcctcggca acgagatctt cgtctacctg
     2881 gacgttcgcg gccaagtgat caccgcccgt atggacccga gcctgagact ccagcgcggc
     2941 cagcagatcg aggttgtggc ggtgccagag cagctccact tcttcgacaa cgagaccgag
     3001 cagacaatca cgccattcgg ttcacggtaa caagcacctc cccggcc
//
LOCUS       DASYWL010000310         4089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_90875, whole genome shotgun sequence.
ACCESSION   DASYWL010000310 DASYWL010000000
VERSION     DASYWL010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90875"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VGR22_10935"
     CDS             complement(<1..497)
                     /locus_tag="VGR22_10935"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129123.1"
                     /translation="MNWTLFFQVIIIGLATGSLVALIALGYTLVYGIIELINFAHGEV
                     FMMGAFFASTLIVLTGVTDQSSGLMIVAVMLLALLASMAFSAALNTGIDRVAYRRLRN
                     APRLAPLIAAIGMSFILLNIGIYWKGSNPITAPSLIPFEYRTYNVLQEWPILRDWFEG
                     STLRLG"
     gene            complement(621..1979)
                     /locus_tag="VGR22_10940"
     CDS             complement(621..1979)
                     /locus_tag="VGR22_10940"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV2129124.1"
                     /translation="MIDARYAGLVEKLRLGEIDRRTFMIRAAALGVSAFVAQASIWSI
                     AAQESDAELRPENLGVEGVEHSTDTSKGTINIYSSWPMTGASEQIGGDSAAAVRFAFE
                     IFGNAAGGFALNYEALDDGIAANNGAWDAAKETENASMAVNDPDCMAYIATYNSGAAE
                     AAIPVTNEAGLAMISPANTAVALTKESPVNPEGYPDVLYASGKRNYMRLVPADDIQGG
                     ASANYAFNSLGTRAYVLHDNQTYGLGVAQVFSDTFEALGGEVLGFEAFDPNAPEYQAL
                     MTKIASEGPDLVYLGAIVNLNASKLLQDMRIQMPADQVAFMGPDGLINQAFIDGAGGA
                     AEGAYITFGGLPPSELESQGGLAKAWYDGMVERVGHVPDSYSVYSFECGVVVAQTIDM
                     VGEKDRAAILDAMFGIEGFRGLIGDWSFTETGDTTLSTISLNTVQDGQITFQEVITPP
                     EE"
     gene            2309..2800
                     /locus_tag="VGR22_10945"
     CDS             2309..2800
                     /locus_tag="VGR22_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129125.1"
                     /translation="MSDQYPFTNAESPEPDRLEEDQQTLEPVRQALRQAGFWAYGTID
                     ESNRWSIAVDDELGRVDVRIGRDGLEIQMRADSPGLYAEEDSPWRRQSRARLARLQIP
                     RIARGFLESHQTAEWDETIEGVVVVETVEMPFQRADDIPAFVKERLPRIEDLVTLIER
                     QLG"
     gene            2824..>4089
                     /locus_tag="VGR22_10950"
     CDS             2824..>4089
                     /locus_tag="VGR22_10950"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV2129126.1"
                     /translation="MAGKEVYREWRRRVRVAQRVEPADTVISGARVLNVFTQQLRTAD
                     IAISDGIIAGVGQYPEARERIELLGAVVAPSFIDAHVHIESSMLWVPQFARATVPRGT
                     GAVLADPHEIANVAGFAGVKAMRKAAQDLPIRVHWMAPSSVPASPLESPGATFDLDDI
                     KRMLAWPECAGLGEMMNFPALLAGEREIFDKIVAANGLPRDGHSPGLRGSAVQAYAAA
                     GIRSDHESTELDEAHDKLAAGMMLVLRQGSSEKNLLDLLPLVEDATWRRCTFGSDDRD
                     AHDLLHNGHVDDVLRTVIAAGLDPVRALTMASWNAAQYMQEWTIGAIAPGFEADLVVL
                     DDDLRNLTVRETWFAGRCVARDGQYLVEEREAPLDTSLTHSVNIAPVHLSDLQLSWED
                     GTRAVGLVPDQIVTRTVEIDPPVRDGQVAA"
BASE COUNT          729 a         1349 c         1307 g          704 t
ORIGIN      
        1 ccgagacgca gcgtggagcc ctcgaaccag tcgcggagga tgggccactc ctgaagcacg
       61 ttataggtgc ggtactcgaa cgggatgagc gatggcgccg tgatcgggtt ggagcccttc
      121 cagtagatac cgatgtttaa caggatgaag ctcatgccga tcgcggcgat cagcggcgcc
      181 agcctcggcg cgtttcgcaa ccttcgatac gcgactcggt cgattccggt gttgagtgcg
      241 gcactgaaag ccatcgatgc gagcagcgcc agcagcatca cggcgacgat catcagaccc
      301 gagctctggt ccgtcactcc ggtgagcacg atgagtgtcg acgcgaagaa ggcgcccatc
      361 atgaagacct cgccgtgcgc gaagttgatc agctcgatga tgccgtagac gagcgtgtag
      421 ccgagcgcga tcagcgccac aagcgagccg gttgccagcc cgatgatgat cacctgaaaa
      481 aagagcgtcc agttcacgac gtttccgtcc cccgtccggt tagcctcgcg accgggtctc
      541 acctcgcccc gtccggttcg cggccagaac agcaggaagg gcgggccggg acagagcccc
      601 gacccgcccg ttgcgacgac ttactcctct ggcggcgtaa tcacttcctg gaaggtgatc
      661 tggccatcct gcaccgtgtt gagcgaaatc gtcgacagcg tggtgtcgcc ggtttcggtg
      721 aagctccagt cgccgatcag gccgcggaag ccctcgatcc cgaacatcgc atcgagaatg
      781 gccgcacggt ccttctcgcc aaccatgtcg atggtctgcg cgaccacgac gccgcactcg
      841 aacgagtaga cggagtagga gtccggcaca tgtccgaccc gctcgaccat cccgtcgtac
      901 caggccttgg cgagaccgcc ctgcgactca agctcagacg gggggagacc accgaaggta
      961 atgtaggcgc cctcagcggc accacccgca ccgtcgatga acgcctggtt gatgaggccg
     1021 tcaggcccca tgaaggccac ctggtcggcc ggcatctgga tgcgcatgtc ctgcagcagc
     1081 ttcgaggcgt tgaggttcac gatcgcgccg aggtagacca gatccggccc ctcggaagcg
     1141 atcttggtca tcagcgcctg gtactccggt gcgttgggat cgaacgcctc gaagccaagc
     1201 acctcgccgc caagcgcctc gaatgtgtcg ctgaagacct gcgcgacgcc gaggccgtag
     1261 gtctgattgt catggagcac gtacgcgcgc gtgcccagcg agttgaacgc gtagttggcc
     1321 gacgcgccac cctgaatatc gtccgccggg accaggcgca tgtagttgcg cttaccgctg
     1381 gcgtacagca catccgggta gccctcggga ttgaccgggc tctccttcgt cagcgccacc
     1441 gccgtgttcg ccggagagat catcgcgaga ccggcctcgt tggtgaccgg gatcgccgcc
     1501 tcggcggctc cggagttgta ggtggcgatg taggccatgc agtccggatc gttgaccgcc
     1561 atcgaggcgt tctcggtctc cttcgccgcg tcccaggcgc cgttgtttgc cgcgatgccg
     1621 tcgtcgagcg cctcgtaatt cagggcgaag ccaccggctg cgttgccgaa gatctcgaac
     1681 gcgaaccgca ccgccgcagc agagtcgccg ccaatctgct ccgacgcgcc cgtcatcggc
     1741 catgaagagt agatgttgat ggtgcccttg ctggtgtccg tgctgtgctc gaccccctcc
     1801 acgccaaggt tctcaggcct gagctcggca tctgactcct gagcagcgat gctccaaatc
     1861 gaggcctgtg cgacgaaggc agaaacgccg agcgccgcag cgcgtatcat gaacgttcgt
     1921 cggtcgatct cgccaagccg gagcttctcg accaaccctg cataccgcgc gtcgatcatg
     1981 tcgtgagtcc tccttgcccg atcgtctcgg gccacactct gcaccggggc cgtccccggt
     2041 accggatgcg ctcgggtaca gcgcaattac cacctcggac ccgatctcac cggcttcagg
     2101 tgccgatcgg accctacctt tttggggttg cggggatagt agcacagccg gcggtcatcg
     2161 taaaaacggg tcgccccagg agcggcggct tgattggagc atcgcgtcgc atgcctgccg
     2221 catctgcggc acactatggg gacaacgcgg cgccggggaa cggcgccggc acagagctgc
     2281 gcgtctattg tccgggagta acttttgcgt gtccgatcag tacccgttca ccaacgccga
     2341 atcacccgag cctgaccgtc tcgaagagga ccagcagacc ctcgagccgg ttcgccaggc
     2401 tctacgccag gccggtttct gggcctatgg caccatcgac gagagcaacc gctggtcaat
     2461 cgcggttgac gacgaactgg gtcgggtcga cgtccgcatc ggccgtgacg gtctcgagat
     2521 ccagatgcgg gccgactctc ccggtctgta cgccgaggag gattcgccgt ggcgccggca
     2581 gtcgcgggct cggctcgcgc gcctgcaaat ccctcgcatc gcgcggggat tccttgaatc
     2641 gcaccagact gccgagtggg acgagacgat cgagggggtg gtggtggtcg agacagtcga
     2701 gatgccgttc cagcgtgcgg acgacattcc cgcgttcgtg aaggagcgcc tcccgcggat
     2761 cgaggatctc gtcacgctca tcgagcggca gcttggctag tccgggccag caggagaagc
     2821 gacatggcgg gcaaagaggt gtaccgggag tggcggaggc gcgtgcgagt cgcccagcgc
     2881 gtggaacccg ccgacaccgt gatctccggc gcgcgcgtac tcaacgtctt cacgcaacag
     2941 ctgaggacgg cggacatcgc gatcagcgac ggcatcatcg ctggcgtcgg ccagtatccc
     3001 gaggcgcgag agcgtatcga gctgctcggt gccgtggttg ctccctcctt catcgacgcg
     3061 cacgtccata tcgagagctc gatgctctgg gtgccgcagt tcgcccgagc caccgtgcca
     3121 agaggaactg gggcggtgct cgctgatccg cacgagatcg ccaacgtcgc cggcttcgcc
     3181 ggtgtcaagg cgatgcgcaa ggcagcgcag gatctgccaa tccgggtcca ctggatggct
     3241 ccatcctccg tgcccgcgag ccccctggaa agccccggcg cgacgttcga cctcgacgac
     3301 atcaagcgca tgctcgcatg gccggaatgt gccgggctcg gcgagatgat gaacttcccc
     3361 gccctgctcg ccggagagcg cgagatcttc gacaagatcg tcgctgccaa cgggcttccc
     3421 cgggacggac acagccccgg acttcgtggc agcgcggtgc aggcctacgc cgccgcgggg
     3481 atccgctccg accacgaatc gacggagttg gacgaggccc acgacaagct ggcagctggc
     3541 atgatgctgg tattgcggca gggttcatcc gaaaaaaacc tcctcgactt gctcccgctg
     3601 gtcgaggatg ccacgtggcg gcgatgcacc ttcggttccg atgaccggga cgcccacgac
     3661 ctgctgcaca acggccacgt cgatgatgtc ctgcgcactg tgatcgctgc cgggctcgac
     3721 ccggttcgcg cgctcaccat ggcaagctgg aacgccgctc agtacatgca agagtggacc
     3781 atcggcgcta tcgctcccgg cttcgaggcc gatctcgtcg ttctcgatga cgatctgcga
     3841 aacctgaccg tgcgggagac atggttcgcg ggccgatgcg tggcccgcga cgggcagtac
     3901 ctggtcgagg agcgcgaagc tccactcgat acctccctca cccactcggt caacatcgcg
     3961 ccggtccatc tctccgatct gcaactttcg tgggaggatg gcacccgcgc agtcggactc
     4021 gtcccggacc agatcgtgac tcgcacagtg gagatcgacc ctccggtccg tgatggccag
     4081 gtcgcggcc
//
LOCUS       DASYWL010000311        11867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_410382, whole genome shotgun sequence.
ACCESSION   DASYWL010000311 DASYWL010000000
VERSION     DASYWL010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 11867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11867
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410382"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VGR22_10955"
     CDS             <1..437
                     /locus_tag="VGR22_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129127.1"
                     /translation="TMLSTWFTRWLNARIEPFLVLPIGAGIVALTALFLALVRDEPWQ
                     MMLAIGVFGLHRDNFRRHAGAHHQGGAAPGDRQRDRAERRTALHRRIDRQRGEHRAPQ
                     QLYPVGARLPTNHGYTIAFIAAAVACLGAVVASVILTPRRMS"
     gene            496..1404
                     /locus_tag="VGR22_10960"
     CDS             496..1404
                     /locus_tag="VGR22_10960"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV2129128.1"
                     /translation="MPTGAFRGVYAIPPTPFHDDGSIDETSLARCIEFCLNAGANGIV
                     APVVASEAAALLDDERMRVAELVTEQVRGRVPVVIGVSGASEPASVRFTRHAGSIGAD
                     AVITMPPGGGQARSEEILAFYTAVARAAAPLPVWIQHYPGLCGTSMAPELLAQLVTEV
                     EGVDWLKEESVHAPQVMTRVRELAGDSLNGVMGGMGGRYLLNEAARGACGTMPACEVV
                     DVHVRIWQALERGDREEARHVHTRLLPLLNFEAMYGCPVWKEVLVRRGVIASARTRIP
                     EAAVLDARSQQELDRLLADLGDLLTV"
     gene            complement(1430..1873)
                     /locus_tag="VGR22_10965"
     CDS             complement(1430..1873)
                     /locus_tag="VGR22_10965"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEV2129129.1"
                     /translation="MQIGWKTVPGNRIAVATLVLVLLLAGAFTVSAQDTATPTAPEVA
                     EPETAEATPVATPILPPLLGGEYPVGTALVTTADSLRLRTQPTTDAETLMTLPAGTEV
                     QVIGGPEQAESFTWYEVEVLDTDEPLTGWIATSADFVDPAQDAVT"
     gene            complement(2147..3394)
                     /gene="clpX"
                     /locus_tag="VGR22_10970"
     CDS             complement(2147..3394)
                     /gene="clpX"
                     /locus_tag="VGR22_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEV2129130.1"
                     /translation="MHYRCSFCNKGQEEVRRLIAGPNSVYICDECVQLCREIIEEEEP
                     ATSSPEPGPSKIPTPRELYDQLNQYVIGQDQAKKILSVAVYNHYKRINAGLQSDSDVE
                     LQKSNILMVGPTGSGKTLLAQTLAKLLDVPFSIADATALTEAGYVGEDVENILLRLIQ
                     SAGDVARAQHGIIYIDEIDKIARKSDNPSITRDVSGEGVQQALLKIIEGTVANVPPQA
                     GRKHPHQEYIQIDTRNILFIVGGAFEGLEDVVRKRTGKQAFMGFGGEGGKATEEIVNP
                     LQHLSHDDLLKYGLIPEFVGRLPVAVALNHLTRQDLMRILTEPKNAAVRQYQRFFALD
                     DVELIFTNDALEATARQAEERKTGARGLRTAIEEVLLDVMYEIPSLEGVKKCIVDANV
                     INNHSRPILLTVNEQEIDYAKTA"
     gene            complement(3452..4090)
                     /locus_tag="VGR22_10975"
     CDS             complement(3452..4090)
                     /locus_tag="VGR22_10975"
                     /inference="COORDINATES: protein motif:HMM:NF009205.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEV2129131.1"
                     /translation="MEIPTIENLIPTVIESTNRGERAFDIYSRLLKDRIIFLGTPIDD
                     QIANLIIAQMLFLEHEDPEQDIRLYINSPGGQVYAGLAIYDTMRMVRPDVQTLCVGMG
                     ASMAAVLLAAGASGKRFALPNSRIMIHQGSAGFRGNVPDIEIAARETMELTKTLTQIM
                     ADHTGKPFDRVREDTQRDYYMTGEQAKEYGIVDQVMMPTAPIGVNGSGKDKA"
     gene            complement(4299..5492)
                     /locus_tag="VGR22_10980"
     CDS             complement(4299..5492)
                     /locus_tag="VGR22_10980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2129132.1"
                     /translation="MRITLDQLDALRMPDGETLLGTIAERDPSPAETLALVSGLRERF
                     DPELVAAAMTIHTLRQRARGRFQDADRLWLTGEGLEQASGDLVADYRAARFAGCIRVA
                     DLGCGIGGDLMALARRDIGSLLAIDRDPLHAAMAALNARTVRPDVDLEIRVADALDVD
                     LTGIDGVFLDPARRRGGQRVGAGGTSPPLDWAIGLVEHVPKVGITTAPGIAHTQVPEG
                     WEFEAIAIGTDLKEGMLWSPALETAHWRATVIDPSSGEAHTLVERGDEPLPITEPLPG
                     MTVIDPNPAVTRAGLVQTLGHQLGARMIDTQIAFLVTEEPVTTAFARSLQIVDALPWH
                     ERRVKMRLRELGAGAVGIRRRGLAGDVDRIARRLRGDGSRRLTVLMTRHRDNPWAIIA
                     EDPPG"
     gene            complement(5497..6651)
                     /locus_tag="VGR22_10985"
     CDS             complement(5497..6651)
                     /locus_tag="VGR22_10985"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV2129133.1"
                     /translation="MTTAPNDVVIVGGGVIGCSIAWHLASRGAGVTLLEQNALASAAS
                     GASAGGVRQQGRDLRELPLAICAIGRWEHLEAELDADLHYHREGHLTLIEDETDLPAV
                     ELSVAQQRAAGLDLHTVVGDDLRDLAPGIADHVTAGTYSEHDGHANPILTTHAFARAA
                     EQAGARIRTGTTVTGIHQQGGRVIGVDTSAGPVSCDHLVLAAGLWIPRLVSGIGINLP
                     IVGFAPQMLATRPMEPRLHQVLGARSRKLSLKQIPTGNYVIGGGWPGDIDLAAGVATP
                     REESIVGSIRDATAVFPELANATLERVWVGVEALTIDEVPILGPLPGVDNLTVAAGFS
                     GHGFALGPIVGQLIAEQILDGTSSLSIDALGLDRFAGAQSGPPKITPKAG"
     gene            complement(6648..7808)
                     /locus_tag="VGR22_10990"
     CDS             complement(6648..7808)
                     /locus_tag="VGR22_10990"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2129134.1"
                     /translation="MTRKPVVVVGAGPAGIAAALAAAASTHCILLDEQSEPGGYALWR
                     TKSAEQTPDVEVIPRLREAGIDYRPSTSVWALFDGPTVAAYASDHAYTIAAAAVVIAT
                     GTVDCVWPVPGWHLNGVFTPRPTLADLRRGAIAPGTRFGIVGNSPQVRSLVEMIKAFG
                     GEISKVTADLTTTRIAGETRVERLGDGRGSATVDHVVLALGRRPDHALALQARAASAF
                     DRQALVEMPLLTGDGATSIPGVFVAGEAAAVRGGDAIRAHGKLGGNSAARVADGTAAP
                     TIPESAPPTKPDVATWPIPSDPETIICREQLVDLATLQQAIRDGAHDVNDLRRRTRAG
                     MGSGQDILPALAALLLHQDPAIPDERLIARVRPPIRPLPFRAVLETLGGRTA"
     gene            complement(7828..8901)
                     /locus_tag="VGR22_10995"
     CDS             complement(7828..8901)
                     /locus_tag="VGR22_10995"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEV2129135.1"
                     /translation="MPARTIRIAVIPGDGIGKEVVPEGLRVLDHLAERSGGSLAFEYE
                     SFPWGCEYYVETGRMMDADGLARLAPFDAIYFGAVGWPGVPDHVSLWGLRLAICQGFD
                     QYACIRPVQLLPGVTSPLRHATEEAMDWVVVRENSEGEYAGIGGRNLAERGEDREVAV
                     QSALFTAQGCERILRYAFELARTRKRKKVTSVTKSNAQQYAMTLWDDTFARVAGDYPD
                     VDADQGLVDAMAARFVTHPDSLEVVVGSNLFGDILSDLGSALAGSLGIAASANIDPER
                     RHPSMFEPVHGSAPDIAGQGIANPIAAIWTVALMLEHLELKDEAATVMDAIRATTASG
                     VLTRDLGGTATTVEVGDSVLSNL"
     gene            9024..10118
                     /locus_tag="VGR22_11000"
     CDS             9024..10118
                     /locus_tag="VGR22_11000"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2129136.1"
                     /translation="MAQSDGIQGIHHITLVSADAQRTVDFYTDVLGLRLVKQTVNFDD
                     PGAYHLYFGDAVGSPGSAITFFEWKNTPQGHPGIGGTHHYALETENRDTLLRWKRRLT
                     DHGLSVSGPLDRHYFTSIYFQDPDGVIIEIATRGPGWTVDEEPDHIGEEHRPPPEEMM
                     VANRDSARIEAETWPEPVDRIDAEMALRHGMHHITAIGTNIERTHGFYSDLLGMRRLK
                     MTDNFDDPGSAHWYWGVGNGAPGTVVTYFERDPKKERRVQMGRGQTHHFALVVPDEES
                     QLALRQRLVDAGFECSPVIDRSYFLSVYTHDPDGHIIELATAGPGFLTDEDEASLGTA
                     LRLPPQLEARRAELEQRLTPLNVSKWRAPD"
     gene            10170..10577
                     /locus_tag="VGR22_11005"
     CDS             10170..10577
                     /locus_tag="VGR22_11005"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2129137.1"
                     /translation="MSKGMRLRMELFVEDMDRTVDFYRDVLGFELARREEGYASVRRG
                     DVILGIGPIAKLPAEGGYFTERRLAQDRGLGVEIVLEVEDVAAEYEQVVATGWPIVEE
                     LQGRPWGLTDFRITDPDGYYLRVTDMTTDGDAR"
     gene            complement(10587..11660)
                     /locus_tag="VGR22_11010"
     CDS             complement(10587..11660)
                     /locus_tag="VGR22_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129138.1"
                     /translation="MRLRTPPARLITLLAALVSLSAAVLPSDAAAHVRWFTPYGPYWD
                     PEWDRFLTLPVLLAVLAVGIVVATLAVAQRLIGDPLWPRPPFLQRMELSAPAILGVQA
                     AIAIIYAATQLDIFVPNIELPRNPLGFAIAGVAIVAGFTYITGVLTRVGALLTVGLFI
                     LALFYGAWYEALEQVIFVGIALYLMAVGRGVVRYGGVQEEDRSPISDWLRPHALRILR
                     WSAGLSVLILGLTEKLLAPELGVAFLQEYPNFNVLGELGIEWFTDRRFVFAAGIVEVT
                     AGAALLSGYLPRVVILVLWIPFNLGIPFLPPTELIGHLPILATMYVLLVRGTEGIPPR
                     EARAWPAQHRAEEIFRRPQPARA"
BASE COUNT         1949 a         3782 c         4069 g         2067 t
ORIGIN      
        1 ggaccatgct gtcgacatgg ttcacacggt ggctgaacgc acgcatcgag ccgttcctgg
       61 tgctgccgat cggtgccggc atcgtcgcgc tgaccgccct gttcctcgcc ctggtgcgtg
      121 acgaaccgtg gcagatgatg ctcgcaattg gcgttttcgg ccttcatcgg gacaactttc
      181 gccgccatgc cggggctcat catcagggcg gtgccgcccc aggagaccgg cagcgcgacc
      241 gggctgaacg ccgtactgcg ctccaccggc ggatcgatcg gcagcgcggc gagcatcgcg
      301 ctcctcagca gctatacccc gttggggcgc ggttgcccac caaccacggc tacacgatcg
      361 ccttcatcgc cgcggcagtt gcctgcctcg gcgcggtcgt ggcgagcgtg atcctcacac
      421 cgcgccggat gtcgtgatcc gtcatgccgc gattgacccg agtcatgcgc cgtcaatgtc
      481 gagagggaga gttgcatgcc gaccggagcg tttcgaggtg tctatgctat cccgccgacc
      541 ccatttcacg acgacggctc gatcgacgag acgtcactcg cgcgctgcat cgagttctgt
      601 ctgaatgccg gtgccaacgg catcgtcgcg ccggtggtcg ccagcgaggc cgccgcactg
      661 ctcgacgacg agcggatgcg cgtggcggaa ctggtgaccg agcaggtgag ggggcgcgtg
      721 ccggtggtga tcggcgtctc gggcgcgagc gaaccggcct cggttcgctt cacgcgtcat
      781 gctggcagca tcggcgccga cgccgtcatc acgatgccgc cgggcggcgg acaggctcga
      841 tcggaggaga tcctcgcctt ctacacggca gtggcccgag ccgcagcgcc gctgccggtc
      901 tggatccagc actatccagg actctgcggc acgtcgatgg cacccgagct cctcgcgcag
      961 ctggtgacag aggtcgaggg tgtcgactgg ctcaaggagg agtctgtcca cgcgccgcag
     1021 gtgatgacgc gggtgaggga gctcgccggg gacagcttaa acggcgtgat gggcggcatg
     1081 ggcgggcgct acctgctcaa tgaggcagcc cggggagcgt gtggcaccat gcccgcgtgt
     1141 gaggtcgtcg atgttcacgt ccgcatctgg caggcgctcg agcggggaga tcgcgaggag
     1201 gcgcgtcatg tccacacgcg cctgctaccg ctgctcaact tcgaagcgat gtatggctgc
     1261 cccgtctgga aggaggtcct ggtgcgccgc ggtgtgatcg cttcggcgcg gacgcgcatc
     1321 cccgaagcag ccgtcctgga tgcgcggagc cagcaggaac tggaccgtct cctggctgat
     1381 cttggcgatc tgctcaccgt ctagcccctg ctacccatcc ttccgaggtt taggtgacgg
     1441 cgtcctgagc cggatcgacg aagtcggcgc tggtcgcgat ccagcccgtg agtggctcat
     1501 cggtgtcgag cacctcaacc tcgtaccaag tgaacgactc agcctgttcg ggaccgccga
     1561 tcacctgcac ctctgtgcct gctggcaacg tcattagtgt ctcggcgtca gtcgtgggct
     1621 gcgtgcgaag gcggaggctg tctgccgtgg tgacgagggc tgtcccgacc gggtactcgc
     1681 cgcccagcaa tggagggaga atcggggtcg cgacaggcgt ggcttctgcg gtttcgggct
     1741 cggcaacctc cggtgcggta ggtgttgctg tgtcctgagc gctcaccgta aaagcgccag
     1801 ccagcagcag gacaagtacc agcgtggcga cggcaatccg gttgcccggc acggttttcc
     1861 atccgatctg catggctctg gctcctggtt tcgaccgact ggttgaccac tcgtggctca
     1921 gtgatagctt cgttagcaga cgccatgcca cgaacaagcc gatgccatct catacaacgg
     1981 tccatggcgc ggagtgcttc gtcggtcctg gccggcacga aggttccaag tggcctccgg
     2041 tgtgggaggt gtgccgggga ggttctgctc gggcaagtga taaacctgaa ccgcccaggg
     2101 ccggatgacc ccgggcggtc ttgtgtcgaa acggtagtgc cgccgactac gccgtcttgg
     2161 cgtagtcgat ctcctgctcg ttcacggtca gcaggatggg gcgcgagtgg ttgttgatca
     2221 cgttcgcgtc gacgatgcac ttcttgacac cctcgagcga gggaatctcg tacatcacgt
     2281 cgagcagcac ctcctcgatg gcggtgcgca gcccgcgagc acctgtcttc cgctcctcgg
     2341 cctgtcgcgc ggtcgcctcg agcgcgtcgt tggtgaagat cagctcgacg tcatcaagcg
     2401 cgaaaaaccg ctggtactgc cgcaccgccg cattcttcgg ctcggtgagg atccgcatca
     2461 ggtcctgccg cgtgaggtgg ttgagcgcca ccgcgaccgg cagacgcccg acgaactcgg
     2521 ggatcagacc gtacttgagc aggtcgtcat gcgagaggtg ctgcagcggg ttgacgatct
     2581 cctcggtggc cttgccaccc tcgccgccga agcccatgaa cgcctgcttg ccggtgcgct
     2641 tgcgcactac atcctcgagg ccctcgaagg cgccaccgac gatgaacagg atgttccggg
     2701 tgtcaatctg gatgtactcc tggtgcgggt gcttgcggcc cgcctgcggc ggcacgttgg
     2761 cgacggtgcc ctcgatgatc ttgagcagcg cctgctgcac gccctcgccg gacacgtcgc
     2821 gggtgatgct cgggttgtcc gacttgcgag cgatcttgtc gatctcgtca atatagatga
     2881 tgccgtgctg cgcgcgggcg acatcgccgg ccgactgaat cagccgcagc aggatgttct
     2941 cgacgtcctc gccgacgtat cccgcctcgg tgagcgcggt ggcatccgca atggagaagg
     3001 gaacgtcgag cagcttggcg agcgtctgcg cgagcagggt cttgccggac ccggtcggcc
     3061 cgaccatcag gatgttgctc ttctggagct cgacatcgct gtcggactgc aggccagcat
     3121 tgatccgctt gtagtggttg tagaccgcca cggagaggat ctttttcgcc tggtcctggc
     3181 cgatgacgta ctgattgagc tggtcgtaga gctcgcgagg tgtggggatt ttcgaggggc
     3241 ccggctcagg actcgacgtg gccggctcct cctcttcaat gatctcgcgg cagagctgga
     3301 cgcactcgtc gcagatgtag accgagttgg gtcccgctat gagccgccga acttcttcct
     3361 gtcctttgtt gcagaaggaa caccgataat gcaccccaac acctcgtttt gctgattacg
     3421 tgagcgttgc ctcttcgcgc cgagcgactg gctacgcctt gtccttccca gacccgttca
     3481 cgccgattgg agcggtgggc atcatcacct ggtcgacgat gccgtactcc ttggcctgct
     3541 cgccggtcat gtagtagtcg cgctgtgtgt cctcgcggac ccggtcgaag ggcttcccgg
     3601 tgtggtcggc catgatctgc gtcagggtct tcgtcaactc catggtctcg cgggcggcaa
     3661 tctcgatgtc gggcacgtta ccgcggaagc ccgccgagcc ctggtgaatc atgatgcgcg
     3721 agttcggcag cgcgaaccgc ttgcccgatg cgccagccgc cagcagcacc gcggccatgc
     3781 tggcgcccat gccaacgcag agtgtctgca cgtccgggcg gaccatgcgc atggtgtcgt
     3841 agatggcgag accggcgtaa acctgaccgc ccggggagtt gatgtagagg cggatgtcct
     3901 gctcggggtc ctcgtgctca aggaagagca tctgggcaat gatcaggttg gcgatctggt
     3961 cgtcgatcgg ggtgcccagg aagatgatgc ggtccttgag cagccgcgag tagatgtcga
     4021 aggcgcgctc gccacggttc gtgctctcga tgaccgtggg gatgaggttc tcgatcgtgg
     4081 ggatttccat agtcgtgtgc atgtcctctg agtaatggag tcgcatcccg ctcgtctctc
     4141 aacaccgcgc agcgacaccc gtaaaaaccg gtgcgttctg cggacaatca tacgcctgcg
     4201 cggcgaggac ctcacctgtc catttggcga ggcggagggg attcgtttgg ccggattcta
     4261 ccatcctggc ggagactccc cctgtagtgc gcgtgttctc acccgggcgg gtcttcggca
     4321 atgatcgccc atgggttatc gcggtgccgg gtcatcagca ccgtcaatcg tcgcgatcca
     4381 tcgccccgta gccgccgggc gattcggtcg acgtccccgg caagtccgcg ccgacggatg
     4441 cctaccgcgc ctgcgccgag ctcccgcaac cgcatcttca cccgccgctc gtgccatgga
     4501 agcgcgtcga cgatttgcaa ggaccgtgcg aacgcagtgg taaccggttc ctccgtgacg
     4561 agaaaggcga tttgcgtgtc gatcatgcgc gcgccgagct ggtgaccgag cgtttggacg
     4621 agccccgcgc gagtgaccgc ggggttgggg tcgatcacgg tcatgccggg cagaggctcg
     4681 gtgatcggca gtggttcgtc accgcgttcg accagggtat gcgcctcgcc gctggaggga
     4741 tcgatcactg tcgcgcgcca atgggcagtc tccagcgccg gcgaccagag catcccttcc
     4801 ttgaggtccg taccgatggc gatggcctcg aactcccacc cctcgggaac ctgcgtgtgg
     4861 gcgatgcccg gcgcggttgt gatgccgacc ttcggtacgt gctcgacgag gccgattgcc
     4921 cagtctagcg ggggagaagt cccgccggcg ccgacgcgct gtccgccgcg gcgccgtgcc
     4981 gggtcgagga agacgccgtc gatgccagtc aggtcgacgt ccagggcgtc ggcgacgcgg
     5041 atctccaaat cgacatctgg ccggacggtg cgggcattga gcgccgccat cgcggcgtgg
     5101 agtgggtcgc gatcaatcgc gagcaggctg ccgatgtccc gtcgcgcgag tgccatcaag
     5161 tcgccgccga tgccgcagcc gaggtcggcc acccgtatgc acccggcgaa gcgagcggcc
     5221 cggtagtcgg cgaccaagtc cccggacgcc tgttcaagtc cctcaccggt cagccagagg
     5281 cgatcggcgt cctggaagcg cccccgggcg cgctggcgga gggtgtggat cgtcattgcg
     5341 gccgcgacca gttcggggtc gaatcgttcg cgcagtccgg agacgagcgc cagcgtctcg
     5401 gcgggggagg ggtcgcgctc ggcgatcgtc cccagcagcg tctcgccgtc tggcatgcgc
     5461 aacgcgtcca actggtcgag ggtgatgcgc atcgcgctag ccggccttcg gggtgatctt
     5521 cggaggtccg gattgcgcac ccgcgaagcg gtcgaggccg agtgcgtcga tcgagagtga
     5581 tgaggtacca tcgaggattt gctcggcgat gagctggccg acgatcgggc cgagggcgaa
     5641 gccgtgaccg gagaagccgg ctgcgactgt gagattgtcg acgcccggaa gtgggccgag
     5701 gatcggcacc tcgtcgatgg tgagcgcttc gacaccaacc cacacccgct cgagggtcgc
     5761 gttggccagc tccgggaaca cggctgtcgc gtcgcggatg ctgccaacga tgctttcctc
     5821 gcgcggggtg gcaacgcctg cggccagatc gatgtctccc ggccacccac cgccgatcac
     5881 gtagttcccc gtgggaatct gcttgaggct caacttgcgt gagcgtgcgc ccagtacctg
     5941 atgcaggcgt ggctccatag ggcgggtcgc cagcatctgt ggcgcgaacc cgacgatcgg
     6001 taggttgatc ccgatccctg acaccagccg cgggatccag agaccggcgg cgagcaccag
     6061 atggtcgcag gacacagggc cagcgctcgt gtcgacgccg atgacgcgcc cgccctgctg
     6121 gtggataccg gtgacggtgg ttccggtgcg aatcctggcg ccggcttgct cggcagctcg
     6181 tgcgaaggcg tgggtggtga ggatgggatt ggcgtgtccg tcgtgttccg agtatgtccc
     6241 ggcggtcaca tgatcagcga tgcccggtgc aagatcgcga agatcatcgc caacgacagt
     6301 gtgcaggtcg agaccggccg cacgctgctg ggcaacgctc agttcgacgg cgggaagatc
     6361 cgtctcgtcc tcgatgagcg tcaggtgtcc ctctcggtgg tagtgcaggt cggcgtccag
     6421 ttcggcctcc aggtgttccc atcggccgat cgcgcagatc gcaagcggca gctcgcgcaa
     6481 atcccgtccc tgctgccgga caccacccgc gctcgcgccg gacgcggccg atgcgagtgc
     6541 gttctgctcg agcagcgtga cgccggcccc ccgcgacgcg aggtgccagg cgatggagca
     6601 gccgatcacg ccgcccccga cgatcacgac gtcgttcggt gcggtcgtca tgcggtccgc
     6661 cctcccaacg tttcgagcac ggcgcggaag ggtagagggc ggatcggtgg ccgcacccgc
     6721 gcgatcagcc gctcgtccgg aatcgccggg tcctgatgca agagcagcgc cgcgagagcc
     6781 ggcaggatgt cttgacctga acccatgcca gcccgcgtac ggcgccgcag atcattgacg
     6841 tcgtgagcgc catcgcgtat cgcctgttgg agtgttgcga ggtcgaccag ctgctcacgg
     6901 cagatgatgg tctcgggatc actcgggatc ggccaagtcg cgacgtcggg tttggtcggt
     6961 ggggcgctct ccgggatggt cggggctgcc gtgccgtccg cgacgcgcgc agcggaattg
     7021 ccccccagct tgccgtgggc gcggatcgcg tccccacccc tgacggcggc agcctcgccc
     7081 gcgacgaaga cccctgggat cgatgtcgcg ccatcgccgg tgagcagggg catctcgacg
     7141 agcgcctgcc ggtcgaacgc actcgctgct cgtgcctgca gcgccagcgc atggtcgggc
     7201 cgtcgcccca gagcgagcac gacatgatca actgtggcgc tccctcggcc gtcgcccagg
     7261 cgctcgacgc gtgtctcccc tgcgatacgc gtggtggtaa ggtcagctgt taccttggat
     7321 atctccccgc cgaacgcttt gatcatctcg accagtgacc ggacttgggg actgttgcca
     7381 acgatgccga accgcgtacc cggcgcgatc gcccctctgc gcagatccgc cagtgtcggg
     7441 cgtggtgtga agacgccatt gaggtgccag cccgggacag gccagacaca gtctacagtt
     7501 ccggtagcga tgacgaccgc cgctgcagcg atcgtatatg catgatcgct ggcgtacgcg
     7561 gcgacggttg ggccgtcgaa gagcgcccaa accgaggtgc tcggtcgata gtcgatcccc
     7621 gcttcgcgta ggcgggggat gacctccacg tcgggtgtct gctcggccga cttggtcctc
     7681 cagagtgcgt agccgcccgg ttccgactgc tcatcgagca ggatgcagtg agtcgatgcg
     7741 gcggcggcca gcgcagcggc gatgccggcg ggtccggcgc cgaccacgac gacaggtttc
     7801 cgagtcaatt gccaccctcc cgattcgtca gaggttggag agcaccgagt cgccgacctc
     7861 gacggttgtt gccgtgccgc cgaggtcacg ggtgagcacg ccggacgccg tcgtcgcgcg
     7921 gatcgcgtcc atcaccgtgg ccgcctcgtc cttcagctcc aagtgctcga gcatcagcgc
     7981 gactgtccag atcgccgcga tcgggttggc gatgccctgc ccggcaatgt ccggtgccga
     8041 gccgtgcacc ggctcgaaca tgctcggatg ccgccgctcc gggtcgatgt tggcgctcgc
     8101 cgcgatgcca agcgatcccg ccagcgcgct gccgaggtcg gagaggatgt cgccgaagag
     8161 gttcgagccg acgacgacct ccagcgaatc cgggtgcgtg acgaaccgcg ccgccatcgc
     8221 atcgacgagc ccctgatcgg cgtcgacgtc ggggtagtcc ccggcgacgc gggcgaaggt
     8281 gtcgtcccag agcgtcatcg catactgctg ggcgttgctc ttggtgacac tcgtgacctt
     8341 cttgcgcttg cgcgtgcgtg cgagctcgaa ggcgtagcgg aggatgcgtt cgcacccttg
     8401 ggcggtgaag agggcggact ggactgccac ctcgcgatcc tcgccgcgct cggcgaggtt
     8461 gcgaccgccg atccccgcgt actcgccctc gctgttctcc cgcaccacga cccagtccat
     8521 ggcctcctcg gtcgcatggc ggagggggct ggtgacgccg ggcagcagtt gcaccgggcg
     8581 gatgcaggcg tattggtcga agccctgaca gatggcgagc cgcagtcccc agagggagac
     8641 gtggtccggc acgccgggcc agccgaccgc accgaagtag atcgcgtcga agggcgcgag
     8701 ccgcgcgagg ccgtcggcgt ccatcattcg ccccgtctcg acgtagtact cgcatcccca
     8761 cgggaacgac tcgtactcga acgcgagcga cccgccggag cgctcagcga ggtggtccag
     8821 cacccggagc ccctcgggga cgacttcctt gccgatgccg tcccccggga tgacggcgat
     8881 gcgaatggtc cgagcgggca tgatggagac gctcctcatg tgagatcgaa tcgtcgggcg
     8941 ctaccatgat ggcacacgtc accgacccgc gagcagcgtt cttgcggacg cgtcggcatg
     9001 atagaccagg aaggagtgta tccatggccc agtctgatgg tattcaggga atccaccaca
     9061 tcacgctcgt cagtgccgac gcgcagcgca cggtcgattt ctacacggac gtgctcggct
     9121 tgcggctcgt gaagcagacc gtcaacttcg acgatcccgg cgcctaccac ctctatttcg
     9181 gcgatgctgt cggctccccc ggctcggcaa tcacgttctt cgagtggaag aacacgccgc
     9241 aggggcatcc gggcatcggc gggacgcatc actacgcgct ggagaccgaa aaccgcgaca
     9301 cattgcttcg ctggaagcga cggctgaccg accacgggct ctcggtgagc ggcccgctcg
     9361 atcggcacta cttcacctcg atctacttcc aggatccgga cggcgtgatc atcgagatcg
     9421 ccacccgggg accggggtgg accgtggacg aggagcctga ccacatcggc gaggagcacc
     9481 ggccgccacc tgaggagatg atggtcgcca accgcgattc tgcccggatc gaggcggaga
     9541 cgtggccgga gccggtcgat cggatcgatg ctgagatggc actgcggcat ggcatgcacc
     9601 atatcacggc gatcggcacg aacatcgagc gtacccatgg cttctacagc gatctgctcg
     9661 gcatgcgacg cctcaagatg accgacaact tcgatgatcc gggttcggca cactggtatt
     9721 ggggcgtcgg taacggtgcg ccgggcacgg ttgtgaccta ctttgagcgg gacccgaaaa
     9781 aggagcgtcg cgtgcagatg ggtcgcggcc agacacatca cttcgcgctg gtggtgcctg
     9841 atgaggagag ccaactcgcc cttcggcagc ggctggtcga tgccgggttc gagtgcagcc
     9901 cggtcattga tcgctcgtac ttcctcagtg tctacaccca cgaccctgat gggcatatca
     9961 tcgagctcgc gacggccggt cccggattcc tgaccgacga ggatgaggcg tcgctgggca
    10021 ccgcgctcag gctgccgccg cagctcgagg cccggcgggc agagcttgag cagcggctga
    10081 caccgctcaa cgtctcgaag tggcgtgcgc ctgactagcg gtagctcaat cggtccatgc
    10141 gttcgaggtg agtgccggag gggcgaacga tgtcgaaggg catgcggctc cgtatggagc
    10201 tcttcgtcga ggatatggac cgcaccgttg acttctatcg cgacgtgctc ggcttcgagc
    10261 tggcgcggcg agaggagggg tacgcgagcg tccggcgcgg ggatgtcatc ctcggcatcg
    10321 ggccgatcgc gaagctgccg gcggagggtg ggtacttcac ggagcgccgg ctggcgcagg
    10381 accgtggtct cggcgtggag atcgtccttg aggtcgagga cgtcgccgcc gagtacgagc
    10441 aggtagtggc caccggctgg ccgatcgtag aggagctcca gggtcggcca tggggactca
    10501 ccgacttccg catcacggac ccggacggtt actacctgcg ggttacggac atgacgacgg
    10561 acggtgatgc acggtgaggt ggtcgatcat gcccgcgcgg gctgcgggcg ccgaaagatc
    10621 tcctcggctc ggtgctgcgc gggccatgcc ctcgcctctc gcggcgggat gccctccgta
    10681 ccgcggacga gcagcacgta catcgtcgcg aggatcggca ggtgcccgat cagctcggtg
    10741 ggcggcagga acgggatgcc gaggttgaac gggatccaga gcacgagaat caccacacgg
    10801 gggaggtacc cgctcagcaa ggcggcgccg gcggtcacct cgacgatacc ggctgcgaag
    10861 acgaagcgac ggtcggtgaa ccactcgatc cccaactccc cgaggacgtt gaagttcggg
    10921 tactcctgca ggaaggccac accaagctcc ggcgcgagga gcttttcggt gaggccgagg
    10981 atgagcaccg acaagccggc gctccagcgc aggatgcgga gggcgtgtgg ccgaagccag
    11041 tcggaaatcg gggatcggtc ctcctcctgc acaccaccat accgcacaac gccacgtccg
    11101 accgccatca ggtagagcgc gattccaaca aaaatgacct gctccagtgc ctcgtaccag
    11161 gcgccgtaga agagggcaag gatgaagagc ccgacggtaa gcagggcccc tacgcgggtg
    11221 agcacgccgg tgatgtacgt gaagccggcg acaatcgcca cgccagcgat tgcgaatcca
    11281 agcggattcc gtggcagttc gatgtttgga acgaaaatgt ccagctgggt ggcggcatag
    11341 atgatcgcga tcgcggcttg cacgccgagg attgccggcg ccgacagttc catccgctgc
    11401 aggaagggtg gacgcggcca gagcgggtcg ccgatcaggc gttgcgcgac tgccagagtt
    11461 gccacgacga tgccgacggc gagcacggcg aggagtaccg gcagcgtgag gaagcgatcc
    11521 cattccgggt cccagtaagg cccgtaaggg gtgaaccatc ggacgtgagc ggcggcatca
    11581 ctcgggagca ccgccgcgga gagtgagacc aacgcggcga gcagagtgat caggcgggca
    11641 ggaggggtgc gtagacgcac ggcatggcct tcatgctgag aacgggtagt gtgcggcgtc
    11701 cgccgcgaat cggcgatagc cgtctcactg agcgcatgat ggatgccaga gcgtgggaag
    11761 ccggaggggc gctgacaaag agtggctggc cccggatccg gggccagcca ctctttgtca
    11821 gcgcccctcc ggcttcccac gctctggcat ccatcatgcg ctcagtg
//
LOCUS       DASYWL010000312        15951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_16674, whole genome shotgun sequence.
ACCESSION   DASYWL010000312 DASYWL010000000
VERSION     DASYWL010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 15951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15951
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16674"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..388)
                     /locus_tag="VGR22_11015"
     CDS             complement(8..388)
                     /locus_tag="VGR22_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129139.1"
                     /translation="MSHQSGDTQPSVAVLTRDLFFGMRIRSVLKQLGYAALLKKTETE
                     FAEATGNTGCALGLIDFNGEVNWDAISPAIEARPDLAVIAFGAHTDTEAFRMARKARV
                     TRVISNGAFSQQLPDLVERYAQRR"
     gene            529..2817
                     /locus_tag="VGR22_11020"
     CDS             529..2817
                     /locus_tag="VGR22_11020"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEV2129140.1"
                     /translation="MASWFSTEGIAGASARRPWLVIAVWLVLLTLSAFYTAENLSSAV
                     STSITFASDPESAVGLEQIDEAGLSEEASIGETIVIASQTNVTVDDPAFQARAEAVVG
                     EVRSLLAEWREMAGLDPEPEVSLGSTPNPVNYYELLQFGIPEVEELVSEDRRYLIVPV
                     SFPPAFFSEVEIGELLDALEALSDDEFEVVTAGTLSINERYSVIAEEDLVRGELIGVP
                     IALLVLVGVLGAIVAPILPILLAVFSIGIALGIVTLLGGVLNLNLFVQNMITMLGLAV
                     GIDYALFVVARYREERQIGYSQQRAIERAGATSSKAVVFSGITVVLALAGVILIPTNL
                     FRSLGLGAVIVVIVAVLANLTLLPAMLGVLGDRINWPRRQRPDDVDREHASEQHAYSG
                     FWGAVTRAVMARPLVSAVVTTTALLALALPVTDIETGFVGPGSLPESDVRTAYDLLRQ
                     EFSAGLLSPVEIVVEGEREEVESALAELVALMETTGDFAPITEAPVWSEDEETVLLDT
                     TLAVPGDSEEAYDVIRWLRHEALPETVGEVGADAWVTGDPAFNLDFITMVNGYTPIVF
                     AFVLGLSFVLLLLAFRSIVVPIKAILMNLLSVGAAYGLMVLIFQKGFMGDTLGFTQTP
                     TIEAWIPIFLFCILFGLSMDYNVFLLSRIREHYDITGRNRESVAVGLRTTARIITGAA
                     MIMVVVFSAFAAGRLVMLQQIGFGLAVAVFIDATIIRSVLVPSAMVLLGDRNWYLPSW
                     LGWLPDVRIESEAPAPSAAADD"
     gene            complement(2950..5253)
                     /gene="serA"
                     /locus_tag="VGR22_11025"
     CDS             complement(2950..5253)
                     /gene="serA"
                     /locus_tag="VGR22_11025"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEV2129141.1"
                     /translation="MTHAQDIDGPRPTSGPARIAIDLDGVLTEHPAPLATAANEKFDA
                     SLPERAFVDSAGLNVPLEIREWVYSDHGPASTLRPAEGAQDFLRKIIQAVGEGNVLII
                     TARPESAAMMTISWLKRNGFPTVNVLFTDDKMTLARRQGCDYAVEDSERHAGNYAAGG
                     ITCFLITRDGGHLSHLDPLIQPVRTFWQILQAMAPVASVRLRRQTVSADLPPMQVDGH
                     HRRPRIVVSDTIHAAARAELEAAADIVDVDGTDVPALLTAVREADALVVRSETQVTSE
                     VLAAAPKLRVVARAGVGVDNINLEQATRAGVLVLNAPGANANSAAEHSVALLLAVTRQ
                     IPFADRTTHAGEWARKLIKPVDLRDRTVGIVGLGRVGGAVAKRLKAFEMRVIAHDPHI
                     PDARFEQLDVEPVSLDEIWDQSDIVTFHVPSTHETRHMLNAETVELLRPGAIVINAAR
                     GDVVDQDALADALRSGRIAGAAVDVFPCEPCHESPLFGLPNVVLTPHTGGSSAEALEA
                     VGRVISSSTLAALRGESVSNGVNLPNAALDAPELKRLSTAATASGKLLAVLTSDVPGD
                     FTLHVRGQVSSSLAELVLGTALAAALQQWLGRRVTPVNARVIATEIGIDVGIVLGDDD
                     PDLMPRFVFSAGGPTSHSVTVSWDRTRAGIIEVDRFSLDRPLSGHVLITHHHDQPGVI
                     GRLGTILGRHNVNIAGMQVGREAPLGEALMVTNVDEAIPEAAMDEIYATEGIEEAYVV
                     ALPTMDENPIEFSAIKEEKRMPAVVGS"
     gene            5503..6621
                     /locus_tag="VGR22_11030"
     CDS             5503..6621
                     /locus_tag="VGR22_11030"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEV2129142.1"
                     /translation="MATAIPTIPRSSHEDRLHRVQQRLAEEGLDWLFLGPSADLFYLT
                     GFDAHVSERLNLLMVPRSGDPTLIVPLLESPNVGEAGQLVSMVTWEDHESPIAAAANV
                     AGSGASGIAVGNHLYSAFLLRLQDAIGADRWVEASPLMRDLRMAKDVVEIELMEIAGR
                     NNDVAWEQFIAAGPISGLTERQALARLLDLTMAQGTGSCWGICASGPNAAAPHYSGSD
                     RVIQPGDSVIFDWGGPIGGYYSDVTRTVHVGEPEEEFRQVYDIVLQANQATLDAVKPG
                     VPCQELDRAARKLITDAGYGEAFLHRVGHGLGLEVHEEPYLVEGNDLALAEGFVFSDE
                     PGIYLEGRFGVRIEDAVVCTADGGRRLNESTRELVVME"
     gene            6633..7880
                     /locus_tag="VGR22_11035"
     CDS             6633..7880
                     /locus_tag="VGR22_11035"
                     /inference="COORDINATES: protein motif:HMM:NF006771.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HEV2129143.1"
                     /translation="MAAKPEVDRMRMLKTLASYAAIGASGNGGVTRLSLTEEDRLARE
                     RFAADAQAAGMSLRVDDMGNMVAHRPGVTDLPPLQLGSHLDTVRRGGRFDGALGVLGG
                     LEVIRALDDAGVTTRHPLELINWTNEEGVRFEPAMMGSGVAYGVFDRAWADDRIDRDS
                     VRFGDALDAIGYRGDVENRPAPGAALLELHVEQGPVLDDADIAVGGVEGVLGITWFNL
                     VIEGQADHAGPSPMPLRRDALATAAEVVTMVERLAIEAGSPAVGTVGRLAPEPNLINV
                     VPGRVEMSVDLRHQSHDELEAFVTRFEAGTRAIGRQRGVSIDIDRFWTSEPTAFHEHI
                     VTAIEGAAAETAPSTMRLWSGAGHDAKYASDAGPAGMIFVRSCGGLSHCEEELSEDDD
                     VIAGVATLLETVLRLDASLDPDG"
     gene            7896..8396
                     /locus_tag="VGR22_11040"
     CDS             7896..8396
                     /locus_tag="VGR22_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129144.1"
                     /translation="MTTEDSYCRQPAFQAEIDHGHDEHTDEELLEQSQLNAQAFILSV
                     FDTVMDDSAQLDRLTAGVARTFARAWDTDRGWEPAEILDALLTNFRSLGGEVDAYEPE
                     EVSPNAVVVELPNADLLEQLGVPVTSFRPMLLVSERLVNLLGCELEWSHDAAEGRIRL
                     QVNSRS"
     gene            8393..8692
                     /locus_tag="VGR22_11045"
     CDS             8393..8692
                     /locus_tag="VGR22_11045"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEV2129145.1"
                     /translation="MTAWIGRARLTLYLHGASSLKDKRSEVKSVIHRLQRRFNVAVAE
                     IEDLDDVRVATIGIVVLSTSAPHADEMLSTILTAAEGMLDMSVPGEVDTELIPFG"
     gene            complement(8791..11751)
                     /locus_tag="VGR22_11050"
     CDS             complement(8791..11751)
                     /locus_tag="VGR22_11050"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HEV2129146.1"
                     /translation="MTGDSLDMANRATQPSDPPYDAHATVAIGRDDLLDRRAIAGGVS
                     WWAVWAGIAMLGAVLFRIVALDLYALSPEEGQLAFQGWAVFRGEGAFEPGELPDTNPF
                     VQLAVALSLFLSGATDVTARLAPVEAGIGMLLLIFLLRPVARPAGVLGLLLTVGLSPS
                     LVYFSRTIDPAIFLAFFIMLWIVAVVRASVARTSGRVVAWAALVGVAMAGLIASGPVG
                     ISAIVAGSIAEAIAIVAPSNERYPDAFGVGGKRVITSPRAIGALVIALLITLGLFFTR
                     LLSDPGAITGIIDTFSEWARIIGTRPTTTPTQFFPWSTLLYEPVAVVLAVVALLAVPV
                     GRLHDGDLLLRGSTFVTWFGVALVLQSLSSGRAPEQFVLISAPLVVAAGIGLGRLLER
                     VERYRLLSSIASLVPFAVLALFVGLIAMLVAVARGNDSAAPGGDTTVQTIIIVLLLVM
                     VPFSAILARAMGGPHGRPIAQWSVLLVLVTLLGIFGIRNASMLAFERAEEGTELLAQN
                     TSTSGVTALVDQITRLSRDVSVGEATAIDPTGRFSIEIALDPDTADPFRWYFREFPEV
                     SVTGPAGWDDADVVIAPATASMAEQGYIVQTRSLFNRVPPAYQTPDTGEILSYLVSPS
                     EWYDGIRFLLFREEVATPPAEQIAVGYTAELTNQINPALGPFNLTDSPGRGSALGQLD
                     TPVGVAHSPDGNIIYVVNSNNLRVERYTEEGEFIGVWSVETDPNLAFASAFGTGPTGI
                     TTGDEGLVYVADTWNHRVVVIDSNGQMVREIGQRGGQVNLNDSPDPDVETGFFFGPRD
                     IAVHDSEIYVTDTGNERVQVFASDGTFLRAFGGFGSGPGQLVEPVGIAVGSDGTVYVA
                     DSGNNRLSIFESDGTPIMQVPVPSWEGQGQLVNYLAIGPDRLLYMTSPSDGTLDVYDP
                     ERNEIILSTSGPETAELEQPIGVAVMPDGEVLVTDTALHGVVRFYPEIPEMPEVPAAV
                     IASPAASPAATPTD"
     gene            complement(11754..14195)
                     /locus_tag="VGR22_11055"
     CDS             complement(11754..14195)
                     /locus_tag="VGR22_11055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase activity-associated protein Agl23"
                     /protein_id="HEV2129147.1"
                     /translation="MTEAPAGTTGRVAETTLPPSTPAATGDGRHAPGEDLSAPAFRIS
                     LTIEHLLWLGLLVIAALTRFWDLGYRTQHHDESIHTTFSWDFATGEAPYVHNPLSHGP
                     FLFHANALIYQLFGGTDATSRFLPALTGVVIVMTPWLLRHRAFLGRWGALATGVFLLF
                     SPAFLYYTRFIRHDPYTALGALLMVVAVFRYLQSPHRKWIILAFVDVAFLLTNHEIAF
                     AILLAFVIVLWTALVLTRLRMLIPVHLAAAALVFLLLAMNRLLEWEPLPEIPWQSGAP
                     EMQRDYYSALLSHPLVIGFIVIGIAFIAGCAWAMRHAARRVAKNGGVIEPLLGDAPQG
                     SVAKGVYDAWNDPIAVGLGALLCIWLYLAFFTTLFTNMTGIATGTYATNGTLLYWLGQ
                     HDVQRGEQPWFYFITEGVQYEWLVIFLGLTGLVALAARLVQRAVRRDFSHDLALLFRM
                     LIAFWMVFMFVVLSWAGEKMPWLIMHIVLPAAVLSGTVVEDFAQGMRRWVHRRLSAHP
                     GGSRPMAAPAALAATLVVLAGSFFLIAANLTWGGYGADLSATARSVPDASLNSWWQLL
                     VPIGLSILAIAVVWAFRGLRPAIYGAVTGALTVMLLFQVHAGFRLSFLEGDVSIDTLI
                     YNTVGPDAKQLALDVEATSELYLGDDSITVSNDGSTNWPHEWYFKDMPNHRFMTSVNT
                     ADPDLPMFIIGVPSEWDSRSSLPMEIPGYTSQTYVFRWHEPEALIYRNFALAPEIPVG
                     RSALQSATQDTGVAAVLESIWSSLWSTTDPDQQQKLWRLLMYRELPGQMSEYRFKVYV
                     DNDALPYYNEVRYGQ"
     gene            14448..14744
                     /locus_tag="VGR22_11060"
     CDS             14448..14744
                     /locus_tag="VGR22_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129148.1"
                     /translation="MNNHDHDKNGTKEAEQIQIARQIGADVTDVFVGFVRGRVPFDEM
                     AFGVFDAMHDLAAVASGDYELETDEDHEHQHYHDHEAAEEQEDLAQEPSRNADA"
     gene            14731..15525
                     /locus_tag="VGR22_11065"
     CDS             14731..15525
                     /locus_tag="VGR22_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129149.1"
                     /translation="MPTPERLEHSDSRSYLHRSLQRRVALIVAGLALVLVIALIPVIT
                     NAELRLEIFRTLGWVPGAEGELVAPADSGATMIVLPIVLELESTALPKRRFLAAYITR
                     PTGDGMWLAPINDGEPFTVPLDDYDLVSSSPDGTEMYVRGEERAVLVDVTEHRVVEML
                     PAGEVPDVAWDWQTATWETGVGRCDLVSNTLTWIGCFERPVLATYLAGDWHLSILRYG
                     GSEEEHDVVRGLGFRPLLGFSADDEWIYLYNENGIRRFSVDEAVED"
BASE COUNT         2836 a         4954 c         5435 g         2726 t
ORIGIN      
        1 caatgtccta cctccgctgc gcgtagcgct ctaccagatc gggcaactgt tgactgaagg
       61 cgccattgga gataacgcgc gttactctag ccttgcgggc catccggaat gcctcggtat
      121 cggtgtgggc accgaacgcg atcacggcga ggtccggccg cgcctcgatt gccgggctga
      181 tcgcgtccca gttgacctcg ccgttgaaat cgatgagccc aagcgcacag cctgtattgc
      241 ctgtcgcctc agcgaactcg gtctcggttt tcttcagcag agcggcgtac cccagttgct
      301 tgagcacact gcgaatccgc atgccgaaga agagatcgcg tgtcaggacc gcgacggacg
      361 gctgtgtatc gccggactga tggctcaccg agatcctcta tggggcggct cagttcctgc
      421 gaaccttccg cttttgacgg tgcttcggcg ctgcaactat catccgcggg gcgcctgtac
      481 cagtatcgcc cgatctccgt ctctcccgca tgatcggagc accgattcat ggcatcctgg
      541 ttctcaaccg agggcatcgc cggggccagc gcccggcgtc cgtggctggt tatcgccgtc
      601 tggctggtcc tgctcaccct ctccgccttc tacacggccg aaaacctgtc cagcgcggtc
      661 tcgacgtcga tcaccttcgc cagcgatccc gagtccgcag ttggcctgga gcagatcgac
      721 gaagctggcc tctccgagga ggcgtcgatc ggcgaaacga tcgtcatcgc atcccaaacc
      781 aatgtcaccg tcgacgaccc ggcgttccaa gcgcgggccg aagcggtggt cggcgaggtc
      841 cgcagcttgc tcgccgagtg gcgggagatg gccggtctcg acccggagcc agaggtgagc
      901 ctgggctcca cgccgaaccc ggtcaactac tatgaactcc tccagttcgg cattcccgaa
      961 gtggaggagt tggtctcgga ggaccggcgc tacctgattg tcccggtctc gttcccgccg
     1021 gcgtttttca gcgaggttga gatcggcgag ctgctcgacg ctctggaggc gctcagcgac
     1081 gacgagttcg aggtggtcac tgccgggacg ttgagcatca acgagcgcta cagcgtgatc
     1141 gccgaggaag atctggtcag gggcgagttg atcggcgttc cgatcgcgct cctggtgctc
     1201 gttggcgtgc tcggggcgat tgtcgcaccc atcttgccga tcctgctggc ggtcttctcg
     1261 atcggcatcg ccctcggcat cgtcacgctg ctcggcggcg tgctcaacct caacctcttc
     1321 gtccagaaca tgattaccat gctcggactc gcggtcggta tcgactacgc gctcttcgtg
     1381 gtagcgcgat accgagagga gcggcagatc ggctactcgc agcagcgggc gatcgagcga
     1441 gcaggggcga cgagcagcaa ggctgtggtc ttctccggca tcaccgtggt cctggcgctg
     1501 gcgggggtca ttctcattcc gacgaacctc ttccggtccc tcgggctcgg cgccgtgatc
     1561 gtggtcatcg ttgccgtgtt ggcaaacctc accctgctgc cggccatgct cggcgtgctc
     1621 ggggaccgga tcaactggcc gcgcaggcag aggccggacg atgtcgatcg cgagcatgcc
     1681 agcgagcagc acgcctacag tggcttctgg ggtgccgtca ccagagccgt gatggcgcgt
     1741 ccgctggtgt cggcggtcgt aacgacaacg gcgctgcttg cccttgcgtt gcccgtaacg
     1801 gacatcgaga cggggttcgt cggcccgggg tccctgccgg agagtgatgt ccggacggca
     1861 tacgatctcc tgcggcagga gttctccgcc gggttgctgt caccggtcga gatcgtcgtc
     1921 gagggcgaac gggaggaggt ggagtcggct ctcgcggagc ttgtcgcgct gatggagacc
     1981 accggcgact tcgcaccgat caccgaggcg ccggtgtggt cggaggacga ggagacggta
     2041 ctgctcgata cgacgctggc ggttcccggc gacagcgagg aggcctacga cgtcatccgc
     2101 tggctgcggc acgaggcatt gccggagacg gtcggggagg tgggcgctga tgcgtgggtg
     2161 acgggtgacc ccgcgttcaa cctcgacttc atcacaatgg tgaacggcta cacgccgatc
     2221 gtcttcgcct tcgtgcttgg cctcagcttc gtgctgctgc tgctcgcttt ccggtcgatc
     2281 gtggtgccaa tcaaggcgat cctgatgaat ctgctctcgg tcggtgccgc ctacggcttg
     2341 atggtcctga tcttccagaa gggattcatg ggcgacaccc tcgggtttac ccagacgccc
     2401 acgatcgagg cgtggattcc gatcttcctc ttctgcatcc tgttcgggct cagcatggac
     2461 tacaacgtct tcctgctgag ccgcatccgc gagcactacg acatcacggg ccgcaaccgt
     2521 gaatccgtcg ccgtcggcct gcgcacaacc gcgcggatta tcaccggcgc agcgatgatt
     2581 atggtggtcg tcttctcggc cttcgccgcc ggtcgcctgg tgatgctgca gcagataggc
     2641 ttcgggctgg cggtggcagt tttcatcgac gcaacgatca tccggtcggt gctcgtgcca
     2701 tcggcgatgg tgctgctcgg cgaccgcaac tggtacctgc cgtcatggct cgggtggctg
     2761 ccggatgtgc gcatcgagag cgaggcccca gctcccagtg ccgctgctga cgactgagta
     2821 gccaattgcc gcgagcgaat cgcacgcccg gagagatcac agatagaccg tccctgcctt
     2881 tgacaggggc gggggatcct tcgctccgga tgacgatgaa ttgggcttgg ataagcggac
     2941 gtttccgggc tatgacccaa ccacggccgg catgcgtttc tcctccttga tcgccgagaa
     3001 ttcgatcggg ttctcgtcca tcgtcgggag cgcgaccacg tacgcctcct caatcccctc
     3061 ggtggcatag atctcgtcca tagctgcctc cggaattgcc tcatcgacat tggtgaccat
     3121 cagcgcttcg ccgagcggtg cctcgcgtcc cacctgcatt ccggcgatgt tgacgttgtg
     3181 gcggccgagg atggtcccga gccgaccgat gacaccgggc tggtcgtggt ggtgggtgat
     3241 cagcacgtgg ccggagagcg gccggtcgag cgagaagcgg tcgacctcaa tgatgcccgc
     3301 ccgcgtgcgg tcccacgaga ccgtcaccga gtggctggtc ggaccgccgg ccgagaagac
     3361 gaagcgcggc atcaggtcag ggtcatcgtc gcccaagacg atgccgacgt cgatgccgat
     3421 ctccgtcgcg atcacccggg cgttgacggg ggtgacgcgc cggccgagcc actgctggag
     3481 cgcggcggcc agcgcggtcc cgagtaccag ctccgcgagg ctgctggaaa cctgcccgcg
     3541 cacgtgcagg gtgaagtcac ccggcacatc ggaggtcagc acggccagca gcttgcccga
     3601 tgcggtggcg gcggtcgaga gccgtttcag ttcaggtgca tcgagggccg cgttcggcag
     3661 gttgacaccg ttcgatacgg actcaccgcg cagggcggca agcgtcgatg agctgatcac
     3721 ccgcccgacc gcctcgagcg cctcggcgct cgacccgccg gtgtgcggcg tcagcaccac
     3781 attggggagg ccgaagaggg ggctctcgtg gcacggctcg cacggaaaca cgtcgacggc
     3841 tgcaccggcg atccggccgc tgcgcaacgc gtcggcgagc gcgtcctggt cgacgacgtc
     3901 gccccgggcc gcgttgatca cgatcgcgcc aggcctgagg agctcgaccg tctcggcgtt
     3961 gagcatgtgc cgggtctcat gcgtcgacgg cacgtggaac gtgacgatgt ccgactgatc
     4021 ccaaatctcg tcgagcgaga ctggctcgac atcgagctgc tcgaatcggg cgtcagggat
     4081 gtgcgggtcg tgggcgatga cgcgcatctc gaacgccttg agtcgcttcg cgaccgctcc
     4141 cccgacacgt ccgagcccca cgatgccgac cgtgcggtcg cggagatcga cgggcttgat
     4201 cagcttgcgg gcccactctc cggcatgggt ggtgcggtca gcgaagggga tttgccgggt
     4261 gacggcgagc agcagcgcga cggaatgctc ggccgccgag ttcgcgttgg cgcccggtgc
     4321 gttcagtacc agcacgcccg cacgggtcgc ctgttccaga ttgatgttgt cgacgcccac
     4381 gccggcgcgc gcgaccacgc gcagcttcgg tgcagctgcg agcacctcgc tggtgacctg
     4441 cgtttcactg cgaacaacga gcgcatcggc ctcgcgtact gccgtgagta gtgcgggcac
     4501 atcagtgccg tctacgtcga cgatatcggc ggccgcctcc agttcggcgc gagcagcggc
     4561 atggatggtg tcactgacga cgatgcgcgg ccgccggtgg tggccgtcga cctgcatcgg
     4621 cgggaggtcg gccgagaccg tctgccggcg tagccggaca ctcgcgactg gcgccatcgc
     4681 ctgcaggatt tgccagaatg tgcggaccgg ctggatcagc gggtcgaggt gggataagtg
     4741 gccaccgtca cgcgtgatga ggaagcaggt gatgccgcca gcagcatagt tgccggcgtg
     4801 gcgctcgctg tcttcaacag catagtcaca gccttgccgt cgagcgagcg tcatcttgtc
     4861 gtcagtgaag aggacgttga ccgtcggaaa accattgcgc ttgagccatg agattgtcat
     4921 catcgcggcg gattccggac gagcggtgat gatcagcacg ttgccctcgc cgaccgcctg
     4981 gatgatcttc cgcaggaagt cctgggcgcc ctcggctggc cgcagcgtcg aagcgggtcc
     5041 gtggtcggag tagacccact cgcggatctc gaggggcacg ttgagaccgg cggagtcgac
     5101 gaaggcgcgc tcgggcaggg aggcgtcgaa cttctcgttg gcggcggtcg ccagcggggc
     5161 cgggtgttcg gtgagaacac catctaggtc aatggcgatg cgtgcgggcc cgcttgtggg
     5221 ccggggtccg tcgatatcct gcgcgtgggt cactgcgcac tgtccttctg atgctcggat
     5281 gcgctcggtg tggatgtcgg cgagggccga cgttgaacac tgtcgaacgg tcaagacgtg
     5341 atgcgaccag ccgcgctcac tcgttccagc ctagcatgac aaaccataaa actgcgactg
     5401 tcggggtcgg agctttacgc ctgtcgccac gtttgcgata aacagcctgt gaacagtgat
     5461 cattgtcctc gtgaatgcga tttgaggcac cggagcacga ccatggccac cgccattccc
     5521 acgattcctc gctcgtccca tgaagatcgg ctgcaccggg tgcagcaacg gctcgccgag
     5581 gaggggctgg actggctctt cctcggaccg tccgcggacc tcttctactt gaccggattc
     5641 gatgcccatg tcagcgagcg gctcaacctg ctgatggtgc cgaggtcggg cgaccccacc
     5701 ctgatcgtgc ccctgctgga gtccccgaac gtcggcgagg cgggccagct tgtcagcatg
     5761 gtgacctggg aggatcacga gtcaccgatc gccgcagccg caaacgttgc agggtcgggc
     5821 gcgagtggga tcgcggtcgg gaatcatctc tacagcgcat tcctgctgcg cttgcaggac
     5881 gcgatcgggg ctgatcgctg ggtcgaagcg agccccttga tgcgcgacct gcggatggcg
     5941 aaggacgtcg tcgagatcga gctgatggag attgccggcc gcaacaacga cgtcgcctgg
     6001 gagcagttca ttgctgccgg gccgatctca ggactgacgg agcggcaggc gctagcccgc
     6061 ctgctggatc tgacaatggc gcagggcacc ggcagctgct ggggtatctg cgccagcggt
     6121 ccgaacgctg ccgcgccgca ctactcgggc tccgatcggg ttatccagcc aggcgacagc
     6181 gtgatcttcg attggggtgg tccgattggc ggctactact ccgacgtcac gcgcactgtc
     6241 cacgtcggtg agccggagga ggagttccgc caggtctacg acatcgtcct gcaggccaat
     6301 caggcaaccc tcgacgccgt aaagccaggt gtcccgtgcc aggagctcga ccgtgctgcc
     6361 cgaaagctga ttaccgacgc aggctatggc gaggcgttcc tgcatcgggt gggacatggt
     6421 ctcggtctcg aggtccacga ggagccctat ctcgtcgagg gaaacgacct ggcgctggcg
     6481 gaaggcttcg tcttcagcga cgagccaggc atctatcttg aaggccgatt cggcgtgcgc
     6541 atcgaggatg cggtcgtgtg caccgccgat gggggccggc ggctcaacga gtcaacgcgc
     6601 gaactggtcg tgatggagta ggggacagca gcatggcggc gaagcccgaa gtcgatcgga
     6661 tgcgcatgct gaagacgctc gcgagctacg cggcgatcgg ggcgagcggc aacggaggcg
     6721 tcacgcgact ctcgctgacg gaggaggacc ggctggcccg tgagcgcttc gctgccgatg
     6781 cgcaagcagc ggggatgtcg ctccgagtgg acgacatggg gaacatggtc gcgcaccgtc
     6841 ctggcgtgac cgatctcccg ccgctgcagc ttggatcgca tctcgacacc gtgcggcgcg
     6901 gtgggcgctt cgacggcgcg ctgggcgtgc tcggtgggct ggaggtcatc cgcgcgctgg
     6961 atgatgccgg cgtcactacc cgccatccgc ttgagctgat caactggacc aacgaggagg
     7021 gggtgcggtt tgagccggcg atgatgggct ccggcgtcgc ctacggcgtc tttgaccgtg
     7081 cttgggcgga cgaccgcatc gaccgtgata gcgttcgctt cggcgatgcg ctcgatgcca
     7141 tcggctatcg gggcgacgtt gaaaaccggc cagcaccggg tgctgccctc ctggagctgc
     7201 acgtggagca gggaccggtg ctcgacgatg cggatatagc ggtcggcggt gtcgaagggg
     7261 tgctcggcat cacgtggttc aacctagtga tcgaggggca ggccgaccat gctggaccaa
     7321 gcccgatgcc gcttcgccgg gacgcgctcg cgacggctgc ggaggtcgtc accatggtgg
     7381 agcgcctcgc catcgaggcc ggctcgccgg ccgtcggcac ggttggacgg ctggcgcccg
     7441 agccgaacct gatcaatgtc gtgccgggca gggtcgagat gagcgtcgat ctgcgacatc
     7501 agtcgcacga cgagctggag gcgttcgtca cgcggtttga agcagggacg cgagcgatcg
     7561 gccgccagcg tggtgtcagt atcgacatcg accggttctg gacaagcgag ccgactgcct
     7621 tccacgagca catcgtcacc gccattgagg gagcggctgc cgaaacggct ccgtcgacca
     7681 tgcgcctgtg gtcgggtgcc ggccatgatg cgaagtacgc ctccgacgcc ggtccggccg
     7741 ggatgatctt cgtgcgcagc tgcggcgggc tcagccactg cgaggaggag ctgagcgagg
     7801 acgacgatgt catcgcgggc gtggcaacat tgctggaaac ggtgcttcgc ctcgacgcat
     7861 cgcttgatcc cgacggttga ccgtttggag ggtacatgac caccgaggac agctactgca
     7921 gacagccggc gtttcaggcc gaaatcgacc acggccatga cgagcacacc gatgaggagc
     7981 tgctcgagca gagccagctg aacgctcagg cgttcatcct gagcgtcttc gataccgtga
     8041 tggatgattc cgcccagctc gaccggttga ccgccggagt cgcgcggacg ttcgcacgag
     8101 cctgggacac cgatcgcggc tgggagccgg ccgagatcct cgacgcactg ctcaccaact
     8161 ttcgctccct aggtggcgag gtggacgcct acgagccaga agaggtctcg ccgaacgctg
     8221 tggtggtcga gttgccgaac gccgacctgc ttgagcaact cggcgtgccg gtgacgtcgt
     8281 tccgcccgat gctcctggtg agcgaacggc ttgtcaacct gctcggctgc gagctggagt
     8341 ggagccacga tgccgccgag gggcgcatcc ggctccaggt caatagccga tcatgacggc
     8401 ctggatcggg cgggcccgcc tgacgctcta cctccatggt gcgtcgtcgc tcaaggacaa
     8461 gcggtcggag gtaaagtcgg tcatccaccg gctgcagcgc cgcttcaatg tcgccgtcgc
     8521 cgagatcgag gaccttgacg atgttcgggt cgcgacgatc ggcatcgtcg tgctgtcgac
     8581 gtcggctccg cacgcggacg agatgctctc gacgatcctc acggccgccg aagggatgct
     8641 cgacatgtcg gttcctggcg aggtcgacac cgagctgatc ccgtttggtt gattcaagct
     8701 aaaccgccgc aatccgccat aggcacgggt gggccatcct gccgacggtc ggatgcagga
     8761 ggacctattc ccgcggctga atcacggtgt tcagtcggtg ggggtggcgg caggcgaggc
     8821 tgcgggtgag gcgatgactg ccgccggaac ctccggcatc tcgggaatct ccgggtagaa
     8881 acgcacgacg ccatgcaagg ccgtgtccgt caccagcacc tcgccgtcgg gcatcacggc
     8941 caccccgatc ggctgttcca gctccgctgt ctccgggccg ctcgtgctga ggatgatctc
     9001 gttgcgctcg gggtcataca cgtcgagcgt tccatcggac ggtgacgtca tatagagcag
     9061 cctgtccggt ccgatggcaa ggtagttgac cagctgtccc tggccctccc acgatgggac
     9121 cggcacctgc atgattggcg tcccatcgct ttcgaagatg ctcaaccgat tgttgccgga
     9181 gtcggcgacg tagacggtgc catccgaacc gacggcgatg ccgaccggct cgaccagctg
     9241 gccgggccca ctgccgaagc cgccgaacgc tcgcaggaag gtaccgtcgc ttgcgaagac
     9301 ctggacacgc tcgttgccag tgtcggtgac gtagatttcg ctgtcatgaa ccgcgatgtc
     9361 gcgcggaccg aagaagaatc cggtctccac atcgggatca gggctgtcgt tcaggttgac
     9421 ctgcccgccg cgctggccga tctcgcggac catctggccg ttgctgtcga tcaccaccac
     9481 gcgatgattc caggtatcgg ccacatagac gagaccttcg tcgccggttg tgatgcccgt
     9541 cgggccagta ccgaaggcgc tggcgaacgc caagttcggg tcggtttcca cgctccagac
     9601 gccaatgaac tcgccctctt cggtgtaccg ctcaacgcgg agattgttgc tgttcacgac
     9661 gtagatgatg ttgccgtcgg gcgagtgggc aacaccgacc ggagtgtcca actgaccgag
     9721 cgcgctgcct cgaccgggcg agtccgtgag gttgaacgga ccgagggcag ggttgatctg
     9781 gttggtcagc tcggcggtgt agccgacggc gatctgctcc gccggcgggg tcgccacctc
     9841 ctcgcggaag agtaggaacc ggatgccgtc gtaccattct gagggggaga ccaggtacga
     9901 taggatctca ccggtatccg gcgtctggta ggcgggcggg acgcggttga acagggaacg
     9961 ggtctggaca atgtagccct gctcggccat tgacgcggtg gcgggggcga tcaccacatc
    10021 agcgtcatcc cagccagccg ggccggtgac acttacctca gggaactcgc ggaagtacca
    10081 ccggaatggg tcggcggtgt caggatcgag agcgatctcg atactgaacc ggccggtcgg
    10141 atcgattgcc gtggcctcgc cgacggagac atcacgcgag agacgggtga tctggtcgac
    10201 gagcgcagtg acgcccgatg tgctggtgtt ctgggcgagc agctcggtgc cctcttcggc
    10261 gcgctcgaag gcgagcatcg aggcgttgcg aatgccgaag atgccgagca gggtcaccag
    10321 caccagcagt accgaccact gcgcgatcgg cctaccgtgc gggccgccca tggcccgagc
    10381 gaggatcgcg gaaaagggca ccatcaccag caagagcacg ataataattg tctgcacggt
    10441 ggtgtcgccg cctggagccg cggagtcgtt gccgcgggca accgccacga gcatggcgat
    10501 gaggccgacg aacaaggcaa gcacggcgaa gggaacgagg ctggcgatgg agctcagcag
    10561 ccggtagcgc tcgactcgtt cgagcaggcg accgagcccg atgccggcgg caaccaccag
    10621 cggcgcgctg atcagcacga actgctctgg agcgcgcccc gatgacaggc tttgcagcac
    10681 gagcgcaacg ccgaaccacg tgacgaaggt cgaaccgcgc aggaggagat cgccgtcgtg
    10741 caggcggccg acaggcaccg cgagcagcgc cacgacggcc agaaccaccg cgaccggctc
    10801 gtagagcagc gtgctccacg ggaagaactg cgttggcgtg gtggtcgggc gcgtgccgat
    10861 aatccgggcc cattcactga aggtgtcaat gatgcccgtg attgcgcctg gatcagagag
    10921 cagccgcgtg aagaagagtc cgagggtgat gagcagcgcg atgacgagag cgccgatcgc
    10981 gcgcggcgag gtgatgacgc gtttcccacc gactccgaac gcatcgggat agcgctcgtt
    11041 tgacggcgcg acgatggcga ttgcctcggc gatcgagcct gcaacgatcg ccgagatccc
    11101 gaccggcccg gaagcgatga gcccggccat tgcgacgccg accagcgcgg cccaggccac
    11161 gacgcggccc gaggtccggg cgacgctggc ccgaacgacc gcgacgatcc acagcatgat
    11221 gaagaacgcg agaaagatcg ccgggtcgat ggtgcgcgag aagtagacga gcgatggtga
    11281 caagccgacg gtgagcagca agccgagcac gccggcgggg cgagccaccg gtcgcagcag
    11341 gaagatcaac agcagcatgc cgattccggc ctccacgggg gcgaggcgcg cggtgacatc
    11401 ggtggcaccc gagagaaaga gcgagagcgc aacggcgagc tgcacgaacg ggttggtatc
    11461 cggcaactcg cccggctcga atgcgccctc gccccgaaag actgcccagc cctggaaagc
    11521 gagttggccc tcctccgggc tcaatgcata gagatccagc gcgacgatcc ggaagagcac
    11581 cgcgccgagc atcgcaatac cggcccacac cgcccaccag gagacaccgc cggcgatggc
    11641 acgccggtcg agcaggtcat cgcgtccgat ggctacggtg gcgtgggcgt catacggcgg
    11701 gtcggagggc tgggttgcgc ggttcgccat gtcgagggag tcacctgtca tcgttactgt
    11761 ccgtaacgga cttcgttgta gtagggcagg gcgtcgttgt cgacgtagac tttgaatcgg
    11821 tattcagaca tctggcccgg caactcgcga tacatcagca ggcgccacag cttctgctgc
    11881 tggtcaggat ccgtggtcga ccagaggctc gaccagatcg actcgagcac ggcggccacg
    11941 cctgtgtcct gcgtggccga ctgcagcgcc gaccgcccga ccggaatctc cggcgcgagc
    12001 gcgaagttgc ggtagatcag cgcctccggt tcgtgccagc ggaagacata cgtctgtgac
    12061 gtatagccgg gaatctccat gggcaagctg gagcggctgt cccactcgga gggcacaccg
    12121 atgatgaaca tcggcaaatc cgggtcggcc gtgttcacgc tggtcatgaa gcggtggttc
    12181 ggcatgtcct tgaagtacca ctcgtgtggc cagttcgtgc tgccgtcatt gctgaccgtt
    12241 atggaatcgt caccgagata cagctccgac gtggcctcga catcgagcgc aagttgcttg
    12301 gcgtccggac cgacggtgtt gtagatgagc gtatcgatcg agacatcgcc ctccagaaac
    12361 gacagacgaa agccggcatg gacctggaag agcagcatga ccgtgagtgc accggtgaca
    12421 gccccataga tggcgggacg caggccacgg aacgcccaga cgaccgcgat ggcgaggatc
    12481 gagagcccga tgggcacaag cagctgccac caggagttga gcgaggcgtc gggcacgctc
    12541 cgcgcggtcg ccgagaggtc ggccccgtat ccaccccaag tgaggttcgc agcgatcagg
    12601 aagaacgagc cggcgagcac caccagcgtc gcggcgagcg cggccggggc cgccatcggc
    12661 cgactgccgc ccgggtgggc ggacaaccgg cggtgaaccc accgtcgcat gccctgtgcg
    12721 aagtcctcca ccaccgtgcc gctcaacacg gcggctggca gcacgatgtg catgatgagc
    12781 catggcatct tctcgccggc ccacgagagg accacgaaca tgaagaccat ccagaaggcg
    12841 atcagcatgc ggaagagcag ggcgaggtcg tgggagaagt cgcgtcgcac cgcccgctgc
    12901 accagccggg cggcgagggc gacgagcccg gtcagcccga gaaagatgac gagccactcg
    12961 tactggacac cctctgtgat gaagtagaac cacggttgct cgccgcgctg gacatcgtgc
    13021 tggcccagcc agtagagcag cgtgccattg gtggcgtagg tacccgtggc gatgccggtc
    13081 atgttggtga agagcgtggt gaagaatgct aagtagagcc agatgcagag cagggcgccg
    13141 agcccgacgg cgatcgggtc gttccaggcg tcataaaccc ccttcgcaac ggagccctgc
    13201 ggggcatcac cgagcagtgg ttcgatcaca ccgccattct tcgccacgcg ccgcgcggca
    13261 tggcgcatcg cccaggcgca cccggcgata aacgcgatgc cgatcacgat gaaaccgatc
    13321 acgagcgggt gggagaggag cgcgctgtag tagtcgcgtt gcatctccgg cgcacccgat
    13381 tgccacggaa tctcgggcag tggttcccac tccagcagcc ggttcatcgc caggaggaga
    13441 aacacgagcg cggcggccgc gaggtggacc gggatcagca tgcgcagccg ggtgagcacc
    13501 agcgcggtcc acagcacgat cacgaaggcc agcaaaatgg cgaaggcgat ctcgtggttg
    13561 gtcagcagaa acgcgacatc aacgaaggcg aggataatcc acttgcgatg tggcgactgc
    13621 aggtagcgaa acaccgcgac caccatcagc agcgcgccca gcgcggtgta gggatcgtgg
    13681 cggatgaagc gggtgtagta gaggaatgcg ggcgaaaaga gcaggaacac cccggtcgcc
    13741 agcgcgcccc agcggccgag aaacgcgcga tgccgcagca gccaaggcgt catcacgatc
    13801 acgacgcccg tcagtgccgg caggaagcgc gaggtcgcgt ctgttccgcc gaagagctgg
    13861 tagatcagcg cgttggcgtg gaagaggaac ggcccgtgcg acagtgggtt gtggacgtat
    13921 ggcgcctcac cggtcgcgaa atcccaggag aaggtggtgt ggatgctctc gtcgtggtgc
    13981 tgcgtccggt accccagatc ccagaatcgg gtgagtgccg cgatgaccag cagcccgagc
    14041 cacagcaggt gctcgatggt cagcgagatt cgaaaggccg gggcggagag gtcttctcct
    14101 ggcgcgtgtc ggccatcacc ggtcgcggcg ggtgttgatg gcggcagcgt ggtctcagcc
    14161 acgcggccgg tcgtcccggc cggtgcttca gtcacggatt tcctccagcg tcatgagatc
    14221 ggggcgcgta tcgcgatatg cgggcacggt agcccgcctc gtggaaccgg gccacaatat
    14281 ggcccggcag aggatacatg ctcgccgata ggatacagtg gaacgccgta gaggtgcagg
    14341 ccagactgta ggccaagacc tcacccagac ggatccgcct ttcttcactt caacgcgggg
    14401 gagccgcgtg gcgttacggc ggaacagacg aattgcgagg agtaacgatg aacaaccacg
    14461 accacgacaa gaacgggacg aaggaagcgg agcagattca gatcgcgcgg cagatcggcg
    14521 cggacgtgac agatgtcttc gtcgggttcg tgcgcgggcg cgtcccgttc gacgagatgg
    14581 cgttcggcgt cttcgatgcg atgcacgatc tcgcggccgt agcgtccggt gattacgaac
    14641 tcgaaaccga cgaggatcat gagcatcagc actaccatga tcacgaggcg gcggaggaac
    14701 aggaggatct cgctcaggaa ccgtcccgga atgccgacgc ctgagaggtt ggagcactcg
    14761 gactctcgca gctacctgca ccgctcgctg caacggcgcg ttgcgctcat cgtcgccggg
    14821 ctggcactcg tcctggtgat cgccctcata ccggtcataa ccaatgcgga gctgcggctc
    14881 gagatcttcc gcacgctggg ctgggttccc ggcgccgagg gcgaactcgt cgcgccggct
    14941 gactccggtg cgacgatgat cgtgctgccg atcgttctcg aactggagtc gaccgccctg
    15001 ccgaagcggc ggttcctggc ggcatacata acccgcccga ccggtgacgg catgtggctg
    15061 gcgccgatca acgatggcga gccatttacg gtcccgctcg acgactatga tctcgtctcg
    15121 agctcaccgg atggaacgga gatgtatgtg cggggcgaag agcgcgccgt gctggtggat
    15181 gtgacggagc atcgcgtggt cgagatgctt ccggcagggg aagtgcccga cgttgcctgg
    15241 gactggcaga cggcaacctg ggagaccggt gtggggcgct gcgaccttgt ctccaacacc
    15301 ctgacgtgga ttggctgctt cgagcggccg gtgctggcca cctaccttgc cggtgactgg
    15361 cacctgagca tcctccgcta cggcggatcg gaggaggaac acgacgtcgt gcgggggctt
    15421 ggctttcgtc cactgctcgg cttttccgca gatgacgagt ggatctacct ctacaatgag
    15481 aacggcattc gtcggttcag cgtcgatgag gccgtcgagg actgacatga acaccgggtg
    15541 atgggtgggc agcccagcca cctgaagtgg gtgcaccttc ggagggtccg caactccaag
    15601 acccgtcttc atgcagccgc cctgatgctg cccgttatgt acccgtgcgg agcgtccaag
    15661 gggtcacaac agagattcac gatcttctca cacgagtggg aatttcccga cagactgcgg
    15721 tgccggccag cggtaaacta tgagtggcgg gtggtgccgg atcaacggcc atcctcgccg
    15781 gtttcagggt ccctgttgac ctgtcttgcg gtacggacac cccggccgca ccatgacgac
    15841 atcacccgcg atgtcaccat cgctcctgtg tcgcactcga agcgtccatg atctcaacac
    15901 tgctgctctc catgctcatc atcgccacct ggctccgcgt ggtccggccc g
//
LOCUS       DASYWL010000313         1828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_185305, whole genome shotgun sequence.
ACCESSION   DASYWL010000313 DASYWL010000000
VERSION     DASYWL010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185305"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..1406
                     /locus_tag="VGR22_11070"
     CDS             228..1406
                     /locus_tag="VGR22_11070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2129150.1"
                     /translation="MQTDREGQLRSHLEAQLTTEWTRLVRLCARLSGNADVAEDLAQE
                     TVTEAWRHAQELRNADALRPWLSGIARNVCLRWWRGQVRDAATLDRFAQTNHEQAASS
                     PAPTSRHELALEQNDLVALLDRALRLLPATTRDVLVQHYINERPHGEIAARLGVSDGA
                     VAVRIHRGKLALRQALARPELSADAVAYGLVDPDDVGWQETHIWCPFCGRHRLAVRIN
                     QKAGVISSRCTGPCFREGTIIGGKWMTAGTSALTSVKSILRRELIDLHECYRKILAAR
                     GGRCESCGHPAALDYWSADVPSSPATPYAHGILLTCRTCGDENSASLSHLLLDMPQVQ
                     QFWRRHPRMQVLPVREIEVHGQAALVSGFASIDASARIEIVSAADTYEILHVDGAVER
                     "
     gene            1403..>1828
                     /locus_tag="VGR22_11075"
     CDS             1403..>1828
                     /locus_tag="VGR22_11075"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2129151.1"
                     /translation="MIATLRQRDFRLAWLGGLISMMGDWVLFIALPIYIYQLTDSTLA
                     TSGMFVAGLLPALLLGSVAGVFVDRWDRKRTMIVCNLILAVILLPLLALRSADWVWIA
                     YVVSFVRSVVSLFFRPAENAFLPRLVAEEDLLAANSLNAF"
BASE COUNT          317 a          568 c          601 g          342 t
ORIGIN      
        1 atctggatgt atgcgagttt acggaagcat ccaacgatgc ttgagcatga agtgatgatg
       61 attgatgtga cgcaaggaaa ggtgatcgtg tcgttccagg gcccacgctt tcctcctcgg
      121 gagatcgaaa aaaacgtgta agaattccgg ctgatccagc gactctaagg tgggacacgc
      181 accgtgtcca ggcgcatcga tgcagtagca gtaatggaga acgggcggtg cagacagacc
      241 gggaagggca actccgttca cacctcgagg cacagctcac cacggagtgg acccggctgg
      301 tgcgtctgtg tgcaaggctc tcgggaaatg ccgacgtggc ggaagatctg gcgcaggaga
      361 ccgtgaccga ggcatggcgc catgcgcagg aacttcgtaa cgcggacgcc ctgcggccgt
      421 ggctctcggg catcgcgcgc aacgtctgtc tccgctggtg gcgcggccag gtccgggacg
      481 ccgcgacgct tgatcgattc gcacagacta accatgaaca ggcagcgagc agcccggcac
      541 caacgagccg gcacgagctc gctctggagc agaacgactt ggtggccctg ctcgaccgcg
      601 ccctgaggct gctcccggcc accacccgcg acgtgctggt ccagcactac atcaatgagc
      661 gcccgcatgg cgagatcgcc gcgaggctcg gcgtcagcga cggcgcggtt gcggtgcgca
      721 tccatcgcgg caagctggcg ctgcgacagg ctctggcgcg gccggaactg agtgccgacg
      781 cggtagccta cgggctcgtc gatccggacg acgttggctg gcaggagact cacatctggt
      841 gccccttctg cggtcggcat cggctcgcag tgcgcatcaa tcagaaggct ggggttatct
      901 cctcccgctg caccggccca tgcttccgcg aggggaccat tatcgggggc aagtggatga
      961 cggcggggac gagcgcgctg accagtgtca agtcgatcct ccggcgggag ctgatcgacc
     1021 tgcacgagtg ttaccggaag atcctggcgg cgcgcggtgg tcgctgcgaa agctgcgggc
     1081 atcctgccgc cctcgactac tggtccgccg acgtgccgtc ctcgccggca actccgtatg
     1141 cccacggcat tctgctgaca tgtcgcacat gcggcgacga gaatagtgcg tcgctcagtc
     1201 atctgctgct cgacatgcca caggttcaac aattctggcg ccggcatcca cgcatgcagg
     1261 tgttgccggt gcgcgagatc gaggtgcatg ggcaggccgc gctggtaagc ggctttgcca
     1321 gcatcgacgc gagcgcaagg atagagatcg tctcagccgc cgacacgtac gagatcttgc
     1381 acgtcgatgg ggctgtcgag cgatgatcgc gacactccgg cagcgtgatt tccgcctcgc
     1441 ctggctcggc gggctcatct cgatgatggg tgactgggtg ctcttcatcg ccctgcccat
     1501 ctacatttac cagttgaccg actcgaccct ggcaacgagc ggcatgttcg tcgccggtct
     1561 tctcccggcg ttgctgctgg ggtcggtcgc gggcgtcttc gttgaccggt gggaccggaa
     1621 gcgcacgatg atcgtgtgca acctcatcct cgcggtcatc ctgcttccgc tgctggcgtt
     1681 gcgctccgcc gactgggtct ggatcgccta cgtggtgagt ttcgtccggt cggtggtttc
     1741 cctgttcttc cgaccagccg agaacgcgtt cctgccgcga ctggtggcag aggaggactt
     1801 gctcgccgcc aattccctca acgcattc
//
LOCUS       DASYWL010000314         3487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_598397, whole genome shotgun sequence.
ACCESSION   DASYWL010000314 DASYWL010000000
VERSION     DASYWL010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598397"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1783)
                     /locus_tag="VGR22_11080"
     CDS             complement(<1..1783)
                     /locus_tag="VGR22_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129152.1"
                     /translation="MTDRLRHVLSIQTLLMLTLVGLGGLAAEAFAPSDVDVRLWAIAV
                     AGGLLGVVLGLLRTPDSVAHLVAIVSGVVLAVGLAALRMPPELSEASYIDRVTSVATV
                     VRDWYLGVGDYADTETMLIVVLLQVITWLVSYLAAWSLMRYGWITVALLLPAGVVAAA
                     RVSKENLPPYLLEIAIVVGIVLLARTTFLRRMGAARPASGIASLLTAIVVALLVLSVG
                     MSTPQDFSANTVEPTVRYATDTVFDAQEQFSDWIADTLGISSNRPPDSNDYPRYTAFD
                     DAFTIGGDLDLTDQPEVLVRTGGDAPYLAAQSYDRYTGRGWESTVEEDFRADGPDGVR
                     YSPELTFRPDQSVPYSDAVNGERVPAEMEITSLLPEAERIYSTGMFLNSSDPASVRFS
                     WRQLDNEIFPLREMEFSALPPDLTGIAALLLRADELAVEGDDGLLYPVSERSREQLAS
                     VRNQLDRRFVDVSWTTGDDGRVESLMVTGQLPVYDDNVAVKPAGDAGEETYRVTCLRT
                     TVDADQLRAASTSYPAWVSGRYLELPDTVTSRTIDLAANLTSGIDNPYDRAKAIELFL
                     RNHIVYDLEVGVAPEDRDIVDYVVFENR"
     gene            complement(1817..3007)
                     /locus_tag="VGR22_11085"
     CDS             complement(1817..3007)
                     /locus_tag="VGR22_11085"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEV2129153.1"
                     /translation="MMNGLKLGLLLLALLVAVTISEWRELEWIVYSGLALLFVAWVWS
                     RASLIGVGFQRTLDADRVFAGTDVREQFRLTNRSHIPKTWVAVTDRSTLPGHAFVRST
                     SIGRRSATCWEVSTQAHQRGRYRLGPVELQSGDPFGLFTNRSQVELSHELLVYPRLVP
                     LPKGERFGAELSGATRVAPGRSAGSPVIRSLRQYVVGDPVNRIAWRSSARHGLLLIKE
                     LEPDPVSDEWILLDLVPQHGRERRATELAVSLGASFAIERLDAARDVGLLVNRCIPAI
                     VDADAGDRQRLRILETLAVVEPYGDADPSSLISRFGARFSRSTNVVVVSTGSGRRHVQ
                     SLRHLLQRGVPVLAIAVGVTENADDGWTEVERLGVEVIRYSASAEPLDGIPARRHVPG
                     TVTA"
     gene            complement(3004..>3487)
                     /locus_tag="VGR22_11090"
     CDS             complement(3004..>3487)
                     /locus_tag="VGR22_11090"
                     /inference="COORDINATES: protein motif:HMM:NF036831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEV2129154.1"
                     /translation="AIGYPGRENEIALLERQHREQPLSRLSSVTTPEEVMRAVQAVRE
                     VHVAAELRTYIVTIVETTRAHTDVFLGASPRGSLALYNLSRGLAWIRGRDYVTPDDIK
                     ALSIPALAHRIVLHPSARMAGHDGREVVSEIVRRVPVPGGATDNGSRRGRVAALRGEG
                     "
BASE COUNT          675 a         1171 c         1097 g          544 t
ORIGIN      
        1 gccggttctc gaagaccaca taatcgacga tgtcccggtc ctctggcgcg acgccgacct
       61 ccaggtcata gacgatgtgg tttcgcagaa agagctcaat ggctttcgca cggtcgtacg
      121 gattgtcgat gccggaggtg aggttggctg ccagatcaat cgtgcggctc gtcacggtat
      181 ccggcagctc gagatagcga ccgctgaccc atgccggata gctcgtggag gcagcccgca
      241 gctgatccgc gtcaaccgtc gtcctcaagc aggtcacgcg ataggtttcc tccccggcat
      301 cccctgccgg cttgacagcc acgttgtcgt catagaccgg caattgcccg gtcaccatca
      361 gcgactcgac tcgaccgtcg tcgccggttg tccagctgac gtcgacgaag cgtcggtcga
      421 gctggttgcg gaccgaggcg agttgctccc gcgaccgctc gctcacgggg tagagcaggc
      481 cgtcgtcgcc ctcaaccgcc agttcatcag cgcgcagcag cagcgcggcg attccggtga
      541 ggtccggagg caaggctgaa aactccatct cccgcagcgg aaaaatctca ttgtcgagtt
      601 gccgccatga gaaccgaact gacgctggat cactgctgtt gaggaacatg ccggtcgagt
      661 agatgcgctc ggcttcgggc aacagcgacg tgatttccat ctcggcgggc acccgctcgc
      721 cgtttactgc gtccgagtac ggcaccgact gatccggcct gaaggtcagc tcgggcgagt
      781 aacgcacgcc atccggcccg tcggcgcgga agtcctcctc aaccgtcgac tcccacccgc
      841 gcccggtata tcggtcgtag ctctgcgccg cgagatacgg cgcgtcaccc ccagttcgta
      901 ccagcacctc cggctggtcg gtcaggtcaa gatcgccacc gatggtgaag gcatcatcga
      961 aggcagtgta ccgcgggtag tcgttggaat ccggtggccg attgctggag atcccgaggg
     1021 tatccgcgat ccaatcggaa aactgctcct gtgcatcaaa gaccgtatcc gtggcatacc
     1081 ggaccgtcgg ttccacggtg ttggcagaga aatcctgggg cgtcgacatg ccaaccgaga
     1141 gcaccagcag ggccaccacg atcgccgtca acagcgaagc gatcccgctt gccggccggg
     1201 cagcccccat gcgacggagg aatgtcgtgc gcgccagcaa cacgatcccc accacgatgg
     1261 cgatctccaa aaggtatggg ggcaggttct ccttgcttac ccgcgccgcc gcgaccaccc
     1321 cagccggcag cagcaacgcc acggtgatcc agccgtaccg catgagtgac cacgctgcca
     1381 ggtaggaaac cagccaggtg atcacctgca acaacaccac gatgagcatc gtctcggtgt
     1441 cggcatagtc cccaacgcct aggtaccagt cgcgaaccac ggtcgcaaca ctcgtgaccc
     1501 ggtcgatata cgatgcttcc gagagctccg gcggcatgcg cagggcggcc aaccccaccg
     1561 cgaggacgac gcccgagacg atagcgacca ggtgggcaac ggagtccggc gtgcgcagga
     1621 gccccagcac gacgccgagc aagcctcccg cgacggcgat ggcccacagt cgcacatcga
     1681 catctgacgg cgcgaacgcc tccgccgcca ggccgccgag gccgaccagc gtgagcatga
     1741 gcagggtctg aatgctgagg acatgtcgca ggcggtcggt cacgggcatt ccaaagcgat
     1801 catgggacga aagtcgttac gcagtcaccg tgccagggac atgccggcga gccggtatgc
     1861 cgtcaagggg ctcggcggac gcagagtacc ggatcacctc gacgccgagg cgttcgacct
     1921 cggtccagcc atcatcggcg ttttcggtca ccccgacggc gatcgcgagc actggcaccc
     1981 cgcgctgcag caagtgtcgc aaggactgca cgtgccgccg gccggagccg gtggagacca
     2041 ccaccacgtt ggtcgagcgg gagaaccggg cgccgaatct ggaaatcagg ctagatgggt
     2101 cagcgtcgcc atagggttct acgacagcga gcgtctccag gattctcaac cgctgccgat
     2161 caccggcatc ggcatcgacg attgccggga tgcaacggtt gacgagcaga ccgacatctc
     2221 gagcggcgtc caggcgttca atggcaaacg acgctccgag cgacacggcc aactccgtcg
     2281 cgcgccgctc gcggccatgc tggggcacga gatcgagcag gatccattca tcggaaacag
     2341 gatccggctc gagctccttg atgagcagca atccatgccg cgccgaggat cgccatgcga
     2401 tacggttcac cgggtctccg accacatatt gccgcaaact ccggatcact ggtgagccgg
     2461 cgctacgccc cggcgccacg cgggtcgcgc cactcagctc cgccccgaac cgctctccct
     2521 tgggcagtgg caccagccgg gggtagacga gcagctcatg gctcagctcc acttggctgc
     2581 ggttggtgaa gagtccgaac ggatcgccgg attgcagctc aacggggccg agccgatacc
     2641 gaccgcgctg gtgggcttgg gttgatactt cccagcaagt cgcagagcgt cgcccgatcg
     2701 acgtcgagcg aacgaaggcg tgtcccggca gggtcgagcg gtccgtcacg gcgacccagg
     2761 tcttcggaat atgcgaccgg ttagtcagcc ggaactgctc gcgcacatcg gtcccggcga
     2821 acacccgatc ggcatcgagg gtacgctgga acccaacccc gatgagggag gcgcggctcc
     2881 agacccaggc gacgaacagc aacgccaggc cgctgtagac gatccattcg agctcccgcc
     2941 actccgagat agtgacggcc acgagcagcg caagcagcag caggcccaac ttgagcccgt
     3001 tcatcatccc tcgcctcgca gcgccgctac cctgccccgg cgcgatccgt tgtcagtggc
     3061 gccgccaggc accgggacgc ggcgcacgat ctcgctgaca acctcccggc cgtcgtgccc
     3121 cgccatgcgt gctgatggat gcaacacgat gcggtgcgcg agcgcgggaa tcgaaagcgc
     3181 cttgatgtcg tcgggcgtta cgtagtcgcg accgcgaatc caggccagcc ctcggctcag
     3241 gttatagagc gcgagcgaac cacgtgggct cgccccgagg aagacatccg tgtgcgcccg
     3301 tgtcgtctcg acaatggtga caatataggt gcgcaactcg gcggctacgt gcacctctcg
     3361 gacggcctgc accgcccgca tgacctcctc aggcgtggtg actgaggaca gtcgcgagag
     3421 tggctgctcc cgatgctggc gctcgagcag cgcgatctcg ttctcgcggc ccggatagcc
     3481 gatggca
//
LOCUS       DASYWL010000315         3371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_672734, whole genome shotgun sequence.
ACCESSION   DASYWL010000315 DASYWL010000000
VERSION     DASYWL010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672734"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1204
                     /locus_tag="VGR22_11095"
     CDS             <1..1204
                     /locus_tag="VGR22_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129155.1"
                     /translation="SGFGCDTTQGPHRDVEYVVARLPMPSVEPEDPMGWLNWRGRWGE
                     RQAWEFDGPVGPRRTRRWTDPVGWHEQLRDSSIAVPGARVFGPAPADVFCSATRYGSI
                     VLIRLMGAPWYFLAMLLAPVIAVGGLLWLAWSTFVSTLRLYLRYLPAFAVLGLPIIPI
                     GIVANAVYDLAIRYSAIRTLVELMEFTPVSYYVAALPFGSIQQLASLLIVVPAVMEIY
                     RSIERGEPITPRTILRGMHAHFRPMLQALPKPLGKVVLAQLTVIGLPWAIERAVRWSF
                     VAPAVLLDDTPPHQAPARSAIAVKGRWWRTAATLLVVTVLGTVPGPLLGIVLLVTESR
                     AIDEINAWSSAIYAVTLPFAILSTAVLYRQDLGQQLPQSAQIPLGEHVPGGKRSLGVL
                     LDESGQPE"
     gene            1279..1545
                     /locus_tag="VGR22_11100"
     CDS             1279..1545
                     /locus_tag="VGR22_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129156.1"
                     /translation="MAAALGAIADPVVEQGEANLRQQVVPGTEIRRRSALLRSFVTSQ
                     LTEGMRSLLVVGAVYEAISGDAAQTRMQLHRDGLLRWCQRGRGR"
     gene            complement(1641..1919)
                     /locus_tag="VGR22_11105"
     CDS             complement(1641..1919)
                     /locus_tag="VGR22_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129157.1"
                     /translation="MYIVAQHSIRDLQKTMEAGQSLFTPPPGYTLHLFLPNAEGGKAI
                     CLWEAESVEAVRELVDGAIGAYSDNQYFAVDEDAAVGLQTITAGTVGD"
     gene            complement(2287..2841)
                     /locus_tag="VGR22_11110"
     CDS             complement(2287..2841)
                     /locus_tag="VGR22_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129158.1"
                     /translation="MRVSFRSLFAILCTVAIALALLPAWGVNAQEATPEAGGTDAIAV
                     VDLAPGITAEVFSGVPTDLAPGQTLYTVRFVFQPGSEIFAHGHPGTTSLVVESGTFGW
                     TLRKGTAHVVRGAASGELTGTEEVTEPGTEVILNPGDAIYYEDDVIHTARGAGDAPTV
                     ILGSMLLESGAPLLHEAAEATPAS"
     gene            complement(2986..3159)
                     /locus_tag="VGR22_11115"
     CDS             complement(2986..3159)
                     /locus_tag="VGR22_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129159.1"
                     /translation="MITETNVMGVPEVRSYQREVIEGDVVICYEVRQAGERDWQPVRL
                     FRAMAGVVQQGRP"
BASE COUNT          551 a         1075 c         1149 g          596 t
ORIGIN      
        1 gtctggcttc ggctgcgaca cgacgcaagg accgcatcgc gacgtggagt atgtcgtggc
       61 gcgactcccc atgccttccg tcgaaccgga ggaccccatg ggatggctga actggcgtgg
      121 acggtggggt gagcggcagg catgggagtt cgacggaccg gttgggccgc ggcgaacacg
      181 gcggtggacc gatccggtgg gctggcacga gcagcttcgg gacagcagca tcgccgtgcc
      241 cggcgccagg gttttcggtc cggcaccggc cgatgtgttc tgctccgcga cccgatacgg
      301 gtcgatcgtg ctgatacggc tcatgggggc gccatggtac ttcctggcga tgctcctggc
      361 accggtgatc gccgtcggcg gtctgctttg gctggcgtgg tcgacgtttg tctcgaccct
      421 gcggctgtac ctgcggtatc tcccggcgtt cgcggtgctt gggctgccga tcataccgat
      481 cggtatcgtg gccaatgccg tgtacgactt ggcaatccgg tattctgcga tccgaacgct
      541 cgtcgagctg atggagttca cgccagtgtc ctattatgtg gcagcgctcc cgttcggcag
      601 catccagcag ctggccagcc tgctgatcgt cgttcccgcc gtaatggaga tttatcggtc
      661 gatcgagcga ggggagccga tcacgccaag aacgatactg cgcggcatgc acgcgcactt
      721 cagaccgatg ctgcaagcgc tgccaaagcc gctcggcaag gttgtactgg cgcagctgac
      781 ggtgatcggg ctgccatggg cgatcgagcg ggcggtgcgg tggagtttcg tggccccagc
      841 ggtcttgctg gacgatacgc caccgcatca agcgccagcc cgaagcgcta ttgccgtcaa
      901 ggggcgttgg tggcgaacgg cagcgacctt gctggtggtt accgtgctcg ggacagtgcc
      961 cggaccactg ctcggaatcg tgctgctggt cacggagtcg cgggcgatcg acgagatcaa
     1021 cgcctggagc agcgcgatct acgcggtgac gctgccgttc gcgattctga gcacggcggt
     1081 gctgtatcgg caggacctgg gacagcaatt accgcaatcg gcgcagatac cgctcggcga
     1141 gcatgtgccg ggagggaaga ggtcattggg ggtactgctg gatgagtctg gacaacctga
     1201 gtagcacgac gtgccagcgg tctcccttgg tggccacccg accctgggta agcactcggc
     1261 gaggagcggg cgcgcgagtt ggccgccgcc ctcggggcga tcgccgatcc ggtggtggag
     1321 cagggcgagg ccaatcttcg gcagcaggtc gtaccgggca ccgaaatccg ccgcaggagt
     1381 gcgctgctcc gctccttcgt gacgagccag ctgaccgagg ggatgcgctc gctgctcgtt
     1441 gtcggcgcgg tttatgaggc catatcggga gatgccgcgc agacccggat gcagctgcac
     1501 cgcgacggct tgcttcgctg gtgccaacga ggccgggggc gatgacagcg cgccggcctc
     1561 gttggcacct ggggccggcg actccatgcc ctacacgttc gacatgaaag cggcccaggt
     1621 gcaccccggg ccgccccgca ctaatcgccg accgtgccgg cggtgatggt ctgcagcccg
     1681 acggctgcgt cctcatccac ggcgaagtac tggttgtcgc tgtaggcgcc gatggcgccg
     1741 tcgaccagct cccgcacggc ctcgaccgac tcggcctccc acaggcagat ggccttgccc
     1801 ccctcggcgt tgggcaggaa caggtggagc gtgtagcccg gcggcggcgt gaacagtgac
     1861 tggcccgcct ccattgtctt ctgcaggtcc cgaatgctgt gctgcgcgac gatgtacatc
     1921 gtggtcctcc tgctttcgga gcgggccacc cggccggccg cggcgtcgct cccggtctgc
     1981 cctcgccccg ggtgagccgg atggcgagct gacgagtcgt tagggttccc atggccagca
     2041 tacacccagc gccgcccatg tcaacggcac gagcagatgg aggcagagca cgatcccctc
     2101 ggcgatcggg atagtggtcg ggatcattgt ggtgatctca aggacgggga acacaagcgg
     2161 ccggccccag gtgaacccga ggccggcgtc gtgtcgatcg gttgtcggtg gatccgtctt
     2221 tgagggagac cgatccgcat gcgggcactg gaggggaagc ccaggcctcc tagccaccgt
     2281 cacgtcttac gaagcggggg tcgcctcagc ggcttcatga aggagcggcg ccccgctttc
     2341 gagcaacatc gatcccagga tcaccgtcgg cgcgtctccg gctccacgcg ccgtgtggat
     2401 aacgtcatcc tcgtagtaaa tcgcgtcgcc ggggttcaag atcacttccg tccctggctc
     2461 ggtcacctcc tcggtcccgg tgagttcgcc actcgccgcg ccgcgcacga catgcgccgt
     2521 gcctttgcgc agggtccacc cgaaggtccc cgactccacg acgagcgagg tggtcccagg
     2581 gtgaccgtgg gcaaaaatct cgctaccggg ctggaagaca aagcggacgg tatagagcgt
     2641 ctggcccggt gccaggtcgg tgggcacgcc ggagaagacc tcggcggtta tgccgggcgc
     2701 gaggtcgacg acggcaatgg cgtcggtgcc gccggcctca ggcgtggcct cctgggcatt
     2761 caccccccag gctggcagca aggccagggc gatggcaacg gtgcagagaa tcgcgaacag
     2821 ggagcggaag gaaacgcgca tgatggaacc tcctgtggat gcgtacctgg ataaacacgc
     2881 atcgataacg gtgtagaccc atgcgacgga tgacggtatt ggctcggtaa cctgggatcc
     2941 ctcagatgag tatacacaaa ctgccgcatg acgaggcgcc tctggtcagg gccggccttg
     3001 ctgtaccacc cctgccatcg cccggaagag ccgcaccggc tgccagtcgc gctcaccggc
     3061 ctggcgcacc tcgtagcaga tcaccacgtc gccctcgatc acctcgcgct ggtagctccg
     3121 cacctccggc acgcccatga cgttcgtctc cgtgatcatg tcgccaccag tgcagcacgc
     3181 cgttgcgggg cagttgccgc agctatccta tgtcaatgtt ggcacgcgtt ctgcttctgg
     3241 tcattcaaga ccggtgggga tcgtcgtcaa ggcgcgcaca cgttcgtcgg gcttgcgtga
     3301 tcggaggcat gggtcagcac cagtcccccg ccgtagcgtt ctcactctct caggacagca
     3361 taccgctcag c
//
LOCUS       DASYWL010000316         8012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_203786, whole genome shotgun sequence.
ACCESSION   DASYWL010000316 DASYWL010000000
VERSION     DASYWL010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8012
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203786"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..491)
                     /locus_tag="VGR22_11120"
     CDS             complement(105..491)
                     /locus_tag="VGR22_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129160.1"
                     /translation="MAVDVLEDLRQRLLPMLQAAALQYANRVPNGYPVVIDSPQTGAV
                     GIEIDPSYALYFTQDADGVSARMYRRSPRTDNTASSGREKFGGAPYSDVRPLGAEVSD
                     QTMRNLIAELMSWYNYQPGVLYITDD"
     gene            complement(640..1224)
                     /gene="dcd"
                     /locus_tag="VGR22_11125"
     CDS             complement(640..1224)
                     /gene="dcd"
                     /locus_tag="VGR22_11125"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEV2129161.1"
                     /translation="MVLSDRDIRKALECGRIRIDPFPDLEKQLGSISVDFRLGRTFMV
                     FEHSRFSYIDPRHPQSIGDAMRTIVAENDEPFIMQPGDFALASTMESLELPDDLLGRL
                     EGRSSIARLGITVHSTAALFEPGWAGTATMELSNLGRMAVALYPGMRICAFSFEPVSS
                     PVSMPYRLKKNNKYAGQRDPKASLLSEENARERE"
     gene            1736..1906
                     /gene="rpmH"
                     /locus_tag="VGR22_11130"
     CDS             1736..1906
                     /gene="rpmH"
                     /locus_tag="VGR22_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEV2129162.1"
                     /translation="MVPKRTYQPKRIPRIREHGFLKRMKTKGGRAVLAARRRKGRHKL
                     TVADEKKFTQNK"
     gene            2006..2449
                     /gene="rnpA"
                     /locus_tag="VGR22_11135"
     CDS             2006..2449
                     /gene="rnpA"
                     /locus_tag="VGR22_11135"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEV2129163.1"
                     /translation="MIARPFRLQKEGDVRRVRSRGKAWAHGAIVARVLPNDLDPPQNR
                     YTVVAGKRTGKAVQRNRLKRLTREALRGYHPWLKPGHDIVFILRGTVEELPDLATAQE
                     ALKRILDKGDLWIVPPGTSGGPPSPGEPVTSGWTRPIPATHEPPS"
     gene            2446..2655
                     /gene="yidD"
                     /locus_tag="VGR22_11140"
     CDS             2446..2655
                     /gene="yidD"
                     /locus_tag="VGR22_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEV2129164.1"
                     /translation="MKRFVLAMLRFYKVAISPSLPPSCRYYPTCSEYAYEAVDKYGIL
                     KGGRLAIWRILRCNPFGGSGYDPVD"
     gene            2792..3919
                     /locus_tag="VGR22_11145"
     CDS             2792..3919
                     /locus_tag="VGR22_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HEV2129165.1"
                     /translation="MLVDVLTTLAPGIPVWDQFREFLEWSLDSIASFTGSAGLAIIVF
                     TIIIKTLLLPLTIKATRSSKAMQEVAPKIKEIQKKYGKDRARASQETMALYQQHGVNP
                     MAGCLPMLIQIPIFFGLYRAIYNLSGDNIGEWGEGFLWMESLAEPDPWHVLPILAGLF
                     QFVQTRMMRPANQPKSDDPQQQIMNVMMNFMPLMVVLFGWTFAAGPVIYWATQSVYSV
                     IQQWFITGWGSLGEWFPWLPELPEHRRLGYREPKPLDEVVVVGGAAAQQKTGFQGWMQ
                     RKMEEAQERQRQVQEQQPTKASTQKTDADDAVAVEADGEQANGEASSPFAAKRRDTST
                     SYRDRVDAAANFAPKSRKPDNGNGSAAPAPPRKSRKKSRKR"
     gene            4006..4803
                     /gene="jag"
                     /locus_tag="VGR22_11150"
     CDS             4006..4803
                     /gene="jag"
                     /locus_tag="VGR22_11150"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HEV2129166.1"
                     /translation="MQRRTSVEIQAYSTEEAIRLALEELQLSRDEVEVEVLVEASDDH
                     EDEALVRVTAKGQASQPEPRAVQRAVGRPGGQNDRLRGRRSPHDRPSRRGTMPAPAVA
                     ERVDAEDEAVAKQVVRDLLDHMGVDADVMAVDNPSSVPLDPDDPPTIFIDVLGSDLGM
                     LIGRRGEHLSHLQHLVNLIVNRKLDTWSRVIIDIEGYRSRREESLIGLAERVARQVSR
                     SHRPVTLEPMPPNERRIIHLTLRAHPDVGTESSGEGTERRITVLPKA"
     gene            4839..5825
                     /locus_tag="VGR22_11155"
     CDS             4839..5825
                     /locus_tag="VGR22_11155"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEV2129167.1"
                     /translation="MSQEPRRDAPESSRRTPNRHERKPFRPGGGRSVTQRMPTPDYLV
                     IGHITVDLLPGGDSVLGGTALYSAMTAARLGARVAVLTRGVFGQEVAGMQIPSLEPYL
                     DERINIIVQDAEVPTAFINEYRADRRVQTMPHWAGPIDLNGLPPHWRNAKIVHLGPIA
                     DELDPRACTGLTSQFLGATPQGWMREWPHSTGGPVKHIPLKLPGALVDRLDSVIVSDE
                     EIVYTREIVNRVGERRLSVMTRGPEGAQVIAHGQKLELPGHRVKVKDLTGAGDVFAAA
                     FFLQAAERGVSAQKAGQFANAVAALSVTEVGPFGVPTRKQVQHLLETGRVRS"
     gene            complement(6050..6436)
                     /locus_tag="VGR22_11160"
     CDS             complement(6050..6436)
                     /locus_tag="VGR22_11160"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2129168.1"
                     /translation="MSFRPAGLDEQSRPPTILIADDEPAIVDLLAQFLEDEGYAVERA
                     GDGQAALELARRLQPDLIIADVMMPRMSGFELVDELTSSDSRMPVILMSAAVISRRDE
                     VAFIAKPFDLGELLDLVESNLQRRVV"
     gene            6611..7258
                     /locus_tag="VGR22_11165"
     CDS             6611..7258
                     /locus_tag="VGR22_11165"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2129169.1"
                     /translation="MPTSLRIQQIPGGPLQTNAFLVVDSNSREALLIDAPPGTHDAIT
                     GAARDAGVTVKAIVLTHTHWDHIVDANALRDALGAPVLAHPKADARLAKPGSAVMQLP
                     FTIAPVTPDGHLEEGDQVMLGSHTFDVLFLPGHDPSHIALYSEAQQALLGGDVLFPGG
                     HGRTDLPDADHRAMLRSLARLLDLPDDIIVYPGHGETMTIGDERNWMTRMTAGIA"
     gene            7279..7764
                     /locus_tag="VGR22_11170"
     CDS             7279..7764
                     /locus_tag="VGR22_11170"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEV2129170.1"
                     /translation="MTPIGIAIERSIRTHVDALLETLQGIPQEDFNTWRPAAAREGNH
                     EMNTFAALAVHAVSAAEFHALHMVGRKAIDRDRDSEFQATAAVPDIEARFAAFLEELH
                     AVLDGMAEADYGGDPAPERPDLEGWTNADWLMHAIDHIALHTGHLQIHRQLWEYETQA
                     G"
BASE COUNT         1484 a         2751 c         2503 g         1274 t
ORIGIN      
        1 gaatctgtac gagataaacg ccgcgaagcc ctccaagccc aggagagagg ccggggccca
       61 cagcgcgggc ctcggcctct tcgtacccca gctggcaccg aggttcaatc gtccgtgatg
      121 tagagaacac cgggctggta gttgtaccag gacatcagct cggcgatcag gttgcgcatc
      181 gtctgatcgc tcacctcggc acccaacggc cggacgtcgc tgtagggcgc gccgccgaat
      241 ttctcccgcc ctgaggatgc cgtgttgtcg gtgcgcgggg agcggcgata catccgcgcc
      301 gagacaccgt cagcatcctg cgtgaagtag agcgcgtaac tcgggtcaat ctcgataccg
      361 accgcccccg tctgcggcga atcgatcacc accgggtagc cgttcggcac gcggttggca
      421 tactgcagcg ccgccgcctg cagcatcggc aagagtcgct gtcgcaaatc ctcaagcaca
      481 tccactgcca cgttgggaac tccttggaac gtgcgagacc gaacgacggc cccacctctc
      541 ggcctggcgg tgtcagaccg attgctcccg agattatggc gcccctccat cgcccttgtc
      601 cacgtggaga gatgtttcac gtgaaacacc gccccggcgt cactcgcgct cgcgagcgtt
      661 ctcctccgac aacaggctgg ccttcgggtc gcgttgcccc gcgtacttgt tgttcttctt
      721 cagtcgatag ggcatcgaga ccggcgagct gactggctcg aacgagaagg cgcagatccg
      781 catgccgggg tagagcgcga cggccatccg tccgaggttg ctgagctcca tggtcgccgt
      841 cccggcccag cccggctcga agagcgccgc cgttgagtga accgtgatgc ccaggcgggc
      901 gatgctggag cgcccctcca gcctgcccag caggtcgtcc ggcagctcca gtgattccat
      961 cgtgctcgcc agcgcgaaat cccccggctg catgatgaac ggctcgtcgt tctcggccac
     1021 gatcgtgcgc atcgcgtcgc cgatcgactg tgggtggcgc gggtcgatgt agctgaagcg
     1081 gctgtgctcg aagaccatga aggtcctgcc gagccggaag tcgaccgaaa tcgatccgag
     1141 ctgcttctcc aggtcgggga acggatcgat gcgaatgcgc ccgcactcca gcgccttgcg
     1201 gatatctcgg tcggaaagaa ccatggtgat tccccacgtc aggtccggca gtcggccagt
     1261 cctcggtcgt cgcagcgccg cccccgctgc ccacaaacgg gtcagggcgg cgagacggac
     1321 tagtcgtacg tacagtaccc aaccggcagc ccgacgtcaa cgcacccggt gcgcatgcca
     1381 acgtccgcga gcgaagcatt cggcagcgct cgacgacaga cgagccacca caaccccact
     1441 caactccgac tcagataatc gcgccaaagc tactgaacgt ggcgcacttg gccagcacat
     1501 tgacgcagcg gccccggatt tcctatactc tcctagcctg tgcatgcggg ttgtgttggt
     1561 atgtccggtg atccccacga tggtgatacc tccagcggac cgacgaccgt gcatgcatgc
     1621 atccctcgtc atcccgcaat cgatgaccag gacacagaat gcgaacgacc gcgcccgtcg
     1681 ccgggcgcag cacatgtgat cggcagcgcc ccgccatcac gcgacaagga gttccatcgt
     1741 gccgaagcgc acctaccagc cgaagcgaat tccgcgcatc cgggagcacg gcttcctcaa
     1801 gcgcatgaag accaagggcg gccgtgccgt gctggcagcc cgccgtcgca agggacggca
     1861 caagctgacg gtggcggacg agaagaagtt cacgcagaac aagtagacgc ttgcactgcg
     1921 gtgtccgggc gagggcgggg gaggccgatg cgctcccgcc ctcgttctgt ttcctgactg
     1981 ggcagcaaca ggggcagcgc atcccgtgat cgcacgtccg ttccggctcc agaaggaagg
     2041 tgatgttcgg cgggtccgat cccgcggcaa ggcgtgggca cacggcgcca tcgtcgcccg
     2101 cgtgctgccg aacgatctcg acccgccaca gaatcgctac accgtggtcg ccggcaagcg
     2161 caccggcaag gcggtccagc gcaaccggct caagcgcctc acgcgcgagg cactgcgcgg
     2221 ctaccacccg tggctcaagc cgggccacga tatcgtcttc atcctgcgcg gaacggtaga
     2281 ggaactgccc gatcttgcca ccgcccaaga ggcgctcaag cgtatccttg acaagggcga
     2341 tctgtggatc gtgccacccg gcacctcagg cggcccgcca tcgcccggtg aacctgtcac
     2401 atccggctgg acgcgaccaa ttccggccac ccatgagcca ccatcatgaa acgcttcgtc
     2461 cttgccatgc tccgcttcta caaggtcgcc atctcgccaa gcctgccacc gagctgccgg
     2521 tactacccaa catgctccga atacgcctac gaggcagtgg ataaatacgg tatcctcaag
     2581 ggcggacggc tcgcgatctg gcgaatcctg cgctgcaatc cgttcggtgg cagcgggtac
     2641 gacccagtgg actgatctga acggcgccga gcgcgcgacc atcgctgtgc ccgcccgtca
     2701 cacctgtgcc gctatcacgc agaaacccgg ataccgggag actccaccgc acccatctca
     2761 cgccggcgtc atctagagga gaacgcacct cgtgctcgtt gatgtcctga ccaccctcgc
     2821 gcccggcatc cccgtctggg atcagttccg ggagttcctg gagtggtcac tcgattccat
     2881 cgccagtttc accggcagcg ctggcctcgc catcatcgtc ttcacgatca tcatcaagac
     2941 gctcctgctg ccgctgacca tcaaggccac gcgctccagc aaggcgatgc aggaggtcgc
     3001 gccgaagatc aaggagatcc agaaaaagta cggcaaggat cgtgcccgcg cctcgcagga
     3061 gacgatggcg ctttaccagc agcacggcgt caacccgatg gccggctgtc tgccgatgct
     3121 gatccagatt cccatcttct tcgggctcta ccgcgccatc tataacctct caggcgacaa
     3181 catcggcgag tggggcgagg gcttcctctg gatggagagc ctggccgagc cggacccatg
     3241 gcatgtgctg ccgatcctgg ccggtctctt ccagttcgtg cagacccgca tgatgcgccc
     3301 ggcaaaccag cccaagagcg atgacccgca gcagcagatc atgaacgtaa tgatgaattt
     3361 catgccgctc atggtcgtgc tcttcggctg gaccttcgcc gcgggcccgg tgatctactg
     3421 ggcgacccag agcgtctact ccgtcatcca gcagtggttc atcaccggct ggggcagcct
     3481 cggtgaatgg ttcccatggc tgccggagct gccggagcac cgccgccttg gctaccgtga
     3541 gccaaagcca ctcgatgagg tggtcgtggt cggtggcgcc gccgcacagc agaagaccgg
     3601 cttccagggc tggatgcagc gcaagatgga agaggctcag gaacgccagc gccaagtcca
     3661 ggagcagcag ccaaccaagg ccagtaccca aaagaccgac gctgacgacg ccgtcgcggt
     3721 cgaagccgac ggggagcagg cgaacggcga ggcgtcatct cccttcgcgg caaaacgacg
     3781 cgacacctcg acctcctacc gtgatcgggt ggacgccgcc gccaacttcg cgccaaaatc
     3841 tcggaagcca gacaacggca atggcagcgc cgcaccggct cccccgagga agtcgcggaa
     3901 gaaaagccgc aagcggtaga gcaagagagc accgggctgc tgctccggcg ggtgtggtct
     3961 attccgcacg gccgacagtg gcatcagaca gggtgagctg agcacatgca gcgacgaacc
     4021 agtgtggaga ttcaggccta ctcgaccgaa gaggccatcc gcctcgcact cgaggagttg
     4081 cagctctcgc gagacgaggt tgaggtcgag gtgctcgtcg aggcgagcga cgaccacgag
     4141 gacgaggcgc tggtccgcgt gaccgcgaag gggcaggcgt cccagccgga gccgcgcgct
     4201 gtccagcgcg ccgtggggcg tcccggcggg cagaacgacc gcctccgcgg tcgccgctcg
     4261 ccgcacgatc gtccatctcg gcgcggcacc atgcccgccc cggccgttgc tgagcgagtc
     4321 gacgccgagg acgaagccgt cgccaagcag gtggtgcgcg atctgctcga ccacatgggt
     4381 gtcgacgccg acgtgatggc ggtcgacaac ccgtcgtcag tgccgctgga ccccgatgat
     4441 ccccccacga tcttcatcga cgtgctcggc agcgacctcg gcatgctgat cggtcgccgc
     4501 ggtgagcatc tctcgcatct gcagcacctc gtcaatctga tcgtgaaccg aaagctcgat
     4561 acgtggtcgc gcgtcatcat cgacatcgag gggtaccgct cccgccgtga ggagtcgctc
     4621 atcggtctgg ccgagcgggt agcgcggcag gtgagccgca gccatcgccc ggtgaccctg
     4681 gagccgatgc cgcccaacga gcgccgcatc atccacctca cgttgcgcgc gcaccccgat
     4741 gtcggcaccg agagcagcgg tgagggcacc gagcgccgca tcactgtgtt gccgaaggcc
     4801 tgacccgccc cgtctccgtc acgactggag ccatcgccat gtcacaggaa cccaggcgcg
     4861 atgcgcccga gtcgtcccgt cgcaccccga atcgccacga gcgcaagccc ttccgccccg
     4921 gtgggggccg ctccgtcacc cagcggatgc ccacccccga ctatctggtc atcgggcaca
     4981 tcactgtgga cctcctgccg ggaggcgact ccgtgctcgg cggcaccgcg ctctactcgg
     5041 cgatgacggc tgcgcggctc ggcgcacggg tcgcggtgct gacccgtggc gtcttcgggc
     5101 aggaggtcgc ggggatgcag attccgtcgc tggagcccta cctcgacgag cgcatcaaca
     5161 tcatcgtcca ggacgccgag gtcccgaccg ccttcatcaa cgagtaccgg gccgaccgcc
     5221 gcgtgcagac gatgccgcac tgggccgggc cgatcgacct caacggcctg ccgccccatt
     5281 ggcgcaacgc gaagatcgtc cacctcggcc cgatcgccga cgagctcgat ccgcgtgcct
     5341 gcacgggcct gacctcgcag tttctcggcg ccaccccgca gggctggatg cgcgagtggc
     5401 cacactcgac cggtggtccg gtcaagcaca ttccgctcaa gctgccgggc gcgctggtgg
     5461 atcgtctcga cagcgtgatc gtcagtgacg aggagatcgt ctacacccgt gagatcgtca
     5521 accgggtggg ggagcgccgc ctgagcgtga tgacacgcgg gcctgaaggc gcacaggtga
     5581 tcgcacatgg ccagaagctc gagctcccgg gacacagggt gaaggtcaag gacctcaccg
     5641 gcgcagggga tgtctttgcg gcggcgttct tcctgcaggc ggccgagcgc ggggtctccg
     5701 cacagaaggc gggccagttc gccaatgcgg tggcagcgct gagtgtgacg gaggttggac
     5761 cgttcggcgt accgacccgc aagcaggtcc agcacctcct cgaaaccggc cgcgttcgct
     5821 cgtaggcacc gccatcacgg ccccacgggc tcgttgtccc tgtcgaactc tcgatggcac
     5881 agagcaatca actggccgtc atgctgaccg ccgactgaag cccgaagcga agggtgacgg
     5941 ctcccgtggg cgttcggcgc ctgccgagga actgtcaggt cgcacgcgaa aatgggcgat
     6001 cccttcgagg aagccgcccg tcgatgttct gttcccctgc ggatgggtcc tagaccacgc
     6061 gccgctgaag gttgctctcg accaagtcga gcagctcgcc gaggtcgaac ggtttggcga
     6121 tgaaagccac ctcgtcccgc cgcgagatga ccgccgcact catcagaatc accggcatcc
     6181 tgctgtcgct gctcgtcaac tcgtcgacca gctcgaatcc gctcatgcgc ggcatcatca
     6241 cgtccgcgat aatgagatcc ggctgcaggc gccgggcaag ctccaacgca gcctgaccat
     6301 cgccggcccg ctctactgcg tacccctcat cctcaaggaa ctgcgcgagc aggtcgacga
     6361 tcgccggttc gtcatcggct atcaagatgg tcggcgggcg cgattgctca tctagacccg
     6421 cgggacgaaa gctcataata gtcccgaatg gctggcgcag catcacgaac ggatgcacat
     6481 cgcacgatgg cgtccgttcc tgtcgctcgt cgcaagttgg gtgccgacgc gcacatgccg
     6541 caccagctac acatcgttct tccggccctc aatacactgc tggggccaca cagcacaggg
     6601 agacacgagc atgccaacat cgctccggat tcagcagatt cccggtggcc cgcttcagac
     6661 caacgcattt ctcgtggtgg actcgaacag ccgcgaggcg cttctgatcg acgcaccgcc
     6721 ggggacgcac gacgccatta caggggcagc ccgcgacgcc ggggtgacgg tgaaggcgat
     6781 cgtgctgacg cacacccact gggaccacat cgtcgacgcc aacgccctgc gggacgcgct
     6841 cggcgcgccg gtcctcgcgc atccgaaggc ggacgctcgc ctcgccaaac ccggcagcgc
     6901 ggtcatgcag ttgcccttca cgatcgcgcc ggtcacgccg gacggccatc tcgaggaggg
     6961 tgaccaagtt atgctcgggt cgcatacctt cgacgtcctt ttcctgccgg gacacgaccc
     7021 atcgcatatc gcgctctaca gcgaagcaca gcaggcactg ctcggcggcg atgtgctctt
     7081 ccccggcggg cacggccgca ctgacctgcc ggacgccgac cacagggcca tgctgcgaag
     7141 tctcgctcgc ctgctcgacc tgccggacga catcatcgtc tatcccggtc acggggaaac
     7201 gatgacgatc ggtgacgagc gcaactggat gacgcggatg accgctggga tcgcctgagc
     7261 cacagggagg gatgtgccat gaccccgatc ggaatcgcca tcgaacgctc catccgcacg
     7321 cacgtcgatg ctctgctgga gacgctgcag ggcatcccgc aggaggactt caacacctgg
     7381 aggccggcgg cagcacgcga gggcaatcac gagatgaata ccttcgccgc actcgcagtc
     7441 cacgccgtct ccgctgccga gttccacgcg ctgcacatgg ttggccgcaa ggcgatcgat
     7501 cgtgatcgcg acagcgagtt ccaggcgacg gcggcggtgc cggacatcga ggcgcgcttc
     7561 gccgccttcc tcgaggagct gcatgctgtg ctggacggga tggcggaggc cgactacggg
     7621 ggcgatccgg cgcccgagcg cccggatctc gagggctgga ccaacgccga ctggctgatg
     7681 cacgccatcg accacatcgc cctgcacacc ggtcacctgc agattcaccg ccagctctgg
     7741 gaatacgaga cccaagcggg gtaggggatc ccgtagaacc agtcattggg ggtgggttct
     7801 gtcgcgattc gagtctggcg acgatcaaga cggtcacagc gaccgtccgc atgcggtgca
     7861 agcccggatg tccagttctc ggtctcaccg tctgattccg ccaacaggta gacccgcact
     7921 ttgatgtgcg ggtttcatcg tggataccat cggcggattc tcgggatcac ctcgcaggca
     7981 acacagaccg ccatgcgcgc tgcgaacggc gg
//
LOCUS       DASYWL010000317         1828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_447992, whole genome shotgun sequence.
ACCESSION   DASYWL010000317 DASYWL010000000
VERSION     DASYWL010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447992"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VGR22_11175"
     CDS             complement(<1..657)
                     /locus_tag="VGR22_11175"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEV2129171.1"
                     /translation="MTRSLRIAHISDTHLGYRALPHQDPLTGRNQRSVDVERSFSAAV
                     DDILRQEVDLVLHAGDVFHQSRPNWHAVRHFVMEWRRIEAAGIPSVVIAGNHDTPRYR
                     AGGSVFSVLEVALPHTRISAGYEEEEITFDELGIRVQTVPHGALTNPDKPVGYAVPEF
                     RNILLIHGMLPGILPSGHVTEPGEQELDTELFEPDFAYIALGHYHIHTSPVSGAWYAG
                     S"
     gene            complement(654..>1828)
                     /locus_tag="VGR22_11180"
     CDS             complement(654..>1828)
                     /locus_tag="VGR22_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129172.1"
                     /translation="GHEPASRMGVVDLDTFGEHSRLNTQRCQPGETDNIYPILWSLKT
                     ALYYPHKVFGYGDLDDKLWGFIYELRERDIDSIDRLEQLFKEIDNHFSDEGTDYWEGH
                     HKATIRKAQNRFKGLQDKLGGLLARGHVQSRTIPTADSPFVDQELRVIDISQANSTAA
                     ELLVSSIINAVWKMAEKAELGVTKLIVFVDELNKYAPAGGEGGLRDTLVDIAARGRHL
                     NVVLFGAQQFRSRVDGEILGNCGTSLYGRVGDEEIINAAYRSISETSKSELLGLPKGR
                     LLVRHAHFREPLFGTFPMPPTIRGIDGQRIFGGEQSSVRKQPADGLHALLQSLLGDHA
                     PRRADVHTECEGIADEAISEVTMKIRREYSGGNHRGNAWGQTMKYLRVKRYGASNR"
BASE COUNT          288 a          573 c          620 g          347 t
ORIGIN      
        1 ggagccggcg taccaggcgc cggagaccgg gctggtgtgg atgtggtagt gtccaagcgc
       61 gatgtaggcg aagtccggct cgaagagctc ggtgtcgagc tcctgctcgc ccggctccgt
      121 cacatgcccc gatggaagga tgcccggcag catgccgtgg atgagcagga tgttccggaa
      181 ttccggaacc gcgtagccga ccggcttgtc cgggttggtg agcgcgccgt gcggcaccgt
      241 ctgcacgcgg atgccgagct catcgaaggt gatctcctcc tcctcgtagc cggccgagat
      301 gcgtgtgtgt ggcagggcca cctcgagcac gctgaacacc gatccgcccg cccggtagcg
      361 cggcgtgtca tggttgccgg cgatgacgac tgaagggatg cccgccgcct cgattcggcg
      421 ccactccatg acgaaatggc ggacggcatg ccagttcggc cggctttgat ggaacacatc
      481 cccggcatgc aggacgagat caacctcctg ccgaaggatg tcgtcgacag cggcgctgaa
      541 cgatcgctcg acgtcgacgg agcgctggtt gcggccggtg agcggatcct ggtggggcag
      601 ggcgcggtac ccgagatggg tatcgctgat gtgtgcgatt cggagcgatc gggtcatcga
      661 ttgctcgccc cgtagcgctt cacgcgcagg tacttcatgg tctgccccca ggcattgccg
      721 cgatgattgc caccggagta ctctcggcgg atcttcattg tgacctcgct gatcgcctcg
      781 tccgcgattc cctcacactc ggtgtggacg tctgcccgcc taggcgcgtg gtcgcccaac
      841 agcgactgca ggagcgcgtg gagcccatct gccggctgct tccgcaccga gctctgttcg
      901 ccgccgaaga tgcgctggcc gtcgatgccc cggatggtcg gtggcatcgg gaaggtaccg
      961 aagagcggtt cgcggaagtg agcgtggcgg accagcaggc gtcccttggg caggccgagg
     1021 agttccgact tcgaggtctc cgagatcgat cggtacgcgg cgttgatgat ctcctcgtcg
     1081 ccgactcgtc cgtagagcga ggtgccgcag ttgccgagga tttcgccgtc gacccgcgac
     1141 cggaactgct gcgcgccgaa gaggacgacg ttgaggtggc gaccacgcgc cgcgatgtcg
     1201 accagcgtgt cacgcaaccc gccctcgccc ccggcgggcg cgtacttgtt gagctcgtcg
     1261 acgaagacga tgagcttcgt gacgccgagc tcagccttct cggccatctt ccagaccgcg
     1321 ttgatgatgg aactcaccag cagctcggcc gcggtcgagt ttgcctgcga gatgtcgatc
     1381 acccgcaact cctggtcgac gaacggcgag tccgccgtcg ggatcgtcct ggattgtacg
     1441 tggccgcgcg caagcaggcc tccgagcttg tcctgcaggc ccttgaagcg gttctgcgcc
     1501 ttgcggatcg tcgccttgtg gtggccctcc cagtagtccg tcccctcgtc gctgaagtgg
     1561 ttatcgatct ccttgaagag ctgctccagg cggtcgatgg agtcgatgtc ccgctcgcga
     1621 agctcgtaga tgaatcccca cagcttgtcg tcgaggtcgc cgtagccgaa gaccttgtgc
     1681 ggatagtaga gcgcggtctt gagcgaccag aggatgggat agatgttgtc ggtctcgccc
     1741 ggctggcaac gctgcgtgtt gagccggctg tgctcgccga acgtatcgag atcgaccacg
     1801 cccattcggc tcgccggttc atgaccgg
//
LOCUS       DASYWL010000318         7081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_260846, whole genome shotgun sequence.
ACCESSION   DASYWL010000318 DASYWL010000000
VERSION     DASYWL010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7081
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260846"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VGR22_11185"
     CDS             complement(<1..247)
                     /locus_tag="VGR22_11185"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEV2129173.1"
                     /translation="MTDRQHAPHHRRVVVTGMGALCALGNTVEPIWNAAVSGASGVAP
                     ITHFDAEGYETTIAGEVKGFEPDEAVGRKGSRRMDRYT"
     gene            571..1578
                     /locus_tag="VGR22_11190"
     CDS             571..1578
                     /locus_tag="VGR22_11190"
                     /inference="COORDINATES: protein motif:HMM:NF017911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129174.1"
                     /translation="MIVIAVGPDASMVRHAVAKHARKADPDGQSTSEMDGNTASMSDV
                     INAIGSAGFFSAGRVVIVHNFISKMNKSGGRGKSAADWDALFSSVPEVSTLILADPTL
                     ASVPAGVRKSLPADAITVEGDPPRGPQLVTWIRKGAESLGSSIDDMAARHLAEQLYPQ
                     TWQRKGNNPAFDRPPDLELLRGEVTKLATAADPGPITHELIDELVARGEDDRIFGFID
                     AVVRGDVSDATTELDRLVAAGEDPHKVLAQLGQTIELTAILTIADGREPVQIGKEIGL
                     SNPNRMTAVARGLRSSSPGRARRAMRLMTTADRMMKTGELRDAIDVIHYVIAGMAPRS
                     R"
     gene            complement(1669..2229)
                     /locus_tag="VGR22_11195"
                     /pseudo
     CDS             complement(1669..2229)
                     /locus_tag="VGR22_11195"
                     /inference="COORDINATES: protein motif:HMM:NF016925.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
     gene            complement(2378..2623)
                     /locus_tag="VGR22_11200"
     CDS             complement(2378..2623)
                     /locus_tag="VGR22_11200"
                     /inference="COORDINATES: protein motif:HMM:NF016925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HEV2129175.1"
                     /translation="MFYHVKELQYEARPEWPDPLFANRLQELVGVKFGEMTVVMSYLF
                     QGWACRGPEKYRDMLLDVGTEEIAHVEMLAVHDRPVA"
     gene            complement(2818..3090)
                     /gene="rpsT"
                     /locus_tag="VGR22_11205"
     CDS             complement(2818..3090)
                     /gene="rpsT"
                     /locus_tag="VGR22_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEV2129176.1"
                     /translation="MANSKSAEKRVRVTARNAERNRRYRSSSRTLVKRAEIAIREGDQ
                     DAAQDAVQHAIAALDRTASKKVIHPNNAARRKSRLMAKFNAMGQQA"
     gene            3305..4981
                     /gene="murJ"
                     /locus_tag="VGR22_11210"
     CDS             3305..4981
                     /gene="murJ"
                     /locus_tag="VGR22_11210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEV2129177.1"
                     /translation="MSQARQLAGSTAPRERDTAAERSTEASPGARSLARSAIIVSLAF
                     VLSRVLGLVREVVLANQFGTTSEMDAYVSAFRIPDLLFLVVIAGSFGAAFIPIFGDFL
                     TRGDEERAWRLASAVLTWAGLVMLALSLLTFVLARPLMMVVAPGFDDETTDLAVELMR
                     ILLLSPIFLGTGIAFKGILEAQHQFTLPALAPLVYNASIIVGAVAFAPTYGIHAVGWA
                     VIAGAVGHMLIQLPGVLRSGLVFTPTISRQVEGLVDVARLLGPRIVGLAAFQVNFIAI
                     NAFASTTSDEHVSALNYAWQLLMLPHGVLALSISTVIFPTLAALYSQGRTEEFRATLD
                     RTLRPLLFLSIPAGIGLYLVREPIVRLIYQSGTFDENSTDLVATPLAFFALGLVGYAL
                     TEILTRVFYATRDTRTPVVTGVLTVALNLLLASLLIDSLSHAGLALALSVTTGAEAVI
                     LWLFLRQRAGGVVSSTFAPWLLRVLLATTVMALLIWLTIETVLEVQTDKEIPLLMREA
                     FFVYALGMYMLAFVVAAWLLRIPELLQITSKAAARLPAPLRGRLPGHIGP"
     gene            5112..6947
                     /gene="lepA"
                     /locus_tag="VGR22_11215"
     CDS             5112..6947
                     /gene="lepA"
                     /locus_tag="VGR22_11215"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEV2129178.1"
                     /translation="MSEQSATSHIRNFCIIAHIDHGKSTLADRLLQRTETVEDRKMKE
                     QLLDSMDLEREKGITIKARAVRMAYTAQDGQKYQFNLIDTPGHVDFSYEVSRSLAASE
                     GALVVVDAAQGIEAQTMANVYLALEHDLAMIAVINKIDLPNADPERVGKEVADFIGLL
                     GEEIIPASAKEGTGIDEILGAIVQTVPPPDNEGIDKPLRALIFDSHYDPYKGVIAYVK
                     IVDGKIDDHDEIRVMGTKKEAGVLELGFFNPDLQATASLNTGEVGYIATGLKEVKDVQ
                     VGDTITLVEKPAETPLKGYRPAQPMVFAGLYPTESDDFPVLRDALDRLQLNDASLTYE
                     PESSAALGFGFRCGFLGLLHMEIIQERLEREYDIDLLATAPSVQYHVRMLNTGEIRKI
                     HNPSDMPPTGEIDAILEPWMHLSIIAPSRYIGTIMELTTSRRAKFIEMIYLDEERVQL
                     KYDIPLAELIVDFYDALKSRTQGYASLDYSYAEMREGNLVKLDVLVNGQAVDALSIIT
                     HRDDAYYKGRELVQKLRGLIPRQMFDVPIQASIGSRVISRETIKAMRKNVLSKCYGGD
                     VSRKRKLLEKQKEGKARMKMVGSVEIPQDAFMAVLSIGSSSDKPQ"
BASE COUNT         1221 a         2374 c         2183 g         1303 t
ORIGIN      
        1 gggtgtagcg gtccatgcgc cgactcccct tccgcccgac ggcctcgtcc ggttcgaacc
       61 ccttcacctc gccggcgatg gtcgtctcgt acccctcggc atcgaagtgc gtgatcggtg
      121 cgacgccgga tgcacctgag acggcggcat tccagatcgg ctcgacggtg tttccgaggg
      181 cgcagagcgc gcccatgcct gtcaccacaa cccgacggtg atgcggtgcg tgctggcggt
      241 cggtcatgct gtcctcgttc cctgcgccga cgcctcggcg ggcgccattc gttcatcccg
      301 ctgattgtcg cacgcacggt agccagacgc attgcatcac ctcccttcca cttggtacac
      361 tgcgtatccg gtcggttcgg gagtgcggct ctgtcggatg tggtcttgag ccaacacttt
      421 cagagccggg agctgctccg cgtgggacgt gtcctgcggc aggctgcggc acccacctag
      481 gtaactaggt tcaaggctcc acatcgcatc cgccccgaac ctgaccatcc cttctcgttc
      541 ccctgacgat gaaacggagc atcgctcgcg atgatcgtga tagccgtcgg ccccgatgcc
      601 tcgatggtgc gccacgccgt ggccaagcac gccaggaagg cagaccccga cggacagtcg
      661 accagcgaga tggacggaaa caccgcctcc atgagcgacg tcatcaatgc gatcggcagt
      721 gccgggttct tctcagcggg ccgggtcgtc atcgtgcaca acttcatttc gaagatgaac
      781 aagtccggcg ggcgtggcaa gagcgcagcc gactgggatg ccctcttttc ttcggtgccg
      841 gaagtctcga cgctcatcct cgccgacccg acgctggcga gcgttcccgc cggcgtgcga
      901 aagtcgctgc ccgccgacgc catcacggtc gagggtgatc cacctcgggg accgcagctc
      961 gtcacttgga tccgcaaggg tgcggagtcg ctcggttcga gcatcgatga catggccgcc
     1021 cgccatctcg ccgagcagct ctatccacag acgtggcagc gcaagggcaa caatccggca
     1081 ttcgatcgac cgcccgacct ggagctgctg cgcggcgagg tgacgaagct tgcgactgct
     1141 gccgatcccg ggccgatcac acatgagctg atcgacgagc tggtcgcgcg cggcgaagac
     1201 gaccggatct tcggcttcat cgacgccgtc gttcggggtg atgtctccga tgcgaccacc
     1261 gagctggatc gcctcgttgc cgccggagaa gacccacaca aggtgctcgc gcagcttggc
     1321 cagacgatcg agctgaccgc aatcctgacc atcgctgacg gccgagagcc ggtccagatt
     1381 ggtaaggaga tcggtctcag caaccccaac cggatgacgg ccgtcgcacg cggactcagg
     1441 agttcgtcgc ccggacgcgc gcggcgggcg atgcgtctga tgaccacagc tgaccggatg
     1501 atgaagaccg gcgagctgcg ggatgcgatc gatgtcatcc actatgtgat tgccggcatg
     1561 gcccctcggt cccgttgaac caccctccgg tacttctgac gccacacctg aaactgccgc
     1621 gcgaatcatt gcccggaggt ttctcggtct ctagcggatc gtgccgtgtt atccggtcgc
     1681 agtcttcttc acgtcgccct tcttgatgtt ggagggaacc tcctgaccct cgcgtgggtt
     1741 cgggccctcg gtcaccgccg gcatcggttc ctttggcgtg ccgtgcaggc gcggatcgcc
     1801 cgtctggagc tctcccttgt cctgcgtcag cggcttggga tcggccaggt actcgaactc
     1861 gccatggccg tccggcgtgt ggcccttggc ccagcgtcct tcctggctct gcgtgccctc
     1921 ggagaggttc cagaacgtgt agagctgatc catctcgatg cgctcgtagg ggaacgggct
     1981 cgggatccgg aactggtgca ggccgtccga ctcgagatct tcgatggcag cctgccactg
     2041 gttctggtgc atgatgtcgc gcgcgaggtt gaacgagagc atgtcgcgca cgccggtgtc
     2101 ctcggtcatc gcgtagagcc gactcacttg caggttgccc tggctctcgg ccataaggtt
     2161 ccaccggaaa tcggccacca agttgccact cgcagcggcg aagtgaccgc tccacggatt
     2221 tccagcgctg tccgtcgcgg cggctcactg accggtcacg atctcatgct gtggattcat
     2281 tgccgcgaag acgacctgct caacgctcga gccgcccatc gccgcttcaa cgacagggtt
     2341 ccgaacagcg tcttcctgtt cgttcgccgg cgccttgtca agcaaccggg cgatcatgga
     2401 cggccagcat ctcgacgtgg gcgatctcct cggtgccgac gtcgagcagc atgtcccggt
     2461 acttctccgg tcctcggcaa gcccaccctt ggaagaggta ggacatcacc acggtcattt
     2521 ccccgaactt gacgccgacc agctcctgca gccggttggc gaagagtggg tcgggccact
     2581 ccggcctggc ttcgtactgc aattccttga cgtgatagaa catacgagaa cctccgcaat
     2641 ggtgcgttca ttggcgcgat cagtctgcgc agggatgtga gggagggagc atgttcggtg
     2701 tcacgagcag gctaacctga tccagcagtc acggactcac acatgaacac gcagaacagc
     2761 ggggcccggt cgttcgggcc ccgctgttct gcgtgttcat gtgattttcg ccaagcgcta
     2821 ggcctgctgg cccatcgcgt tgaacttcgc catcagccgg ctcttccgac gggcggcgtt
     2881 attcggatgg atcaccttct tcgaggcggt acggtccagc gcggcgatgg cgtgctgcac
     2941 ggcatcctgt gcggcgtcct ggtcaccctc gcggatcgca atctcggctc gcttgacgag
     3001 cgtgcgggac gacgaccggt aacggcggtt gcgctcggcg ttgcgggccg tgacccgaac
     3061 gcgcttctcg gcggatttgc tgtttgccac tggtaccctt cctgacgatt gtgtgcgtcg
     3121 aattctgtcc tgatgatcat cgacagcacg cgcgggcagg atcacggctg tcagcgtagc
     3181 ggacacagca agaaagagac ccaggacggg ccttcgcggt attctacctc atggccgcgc
     3241 ccatcctcaa gcgccatgcc gcgccgaggc gccgagaccg cagcaaccag cacacaggga
     3301 caccatgagc caggcacgtc aactcgccgg atcgaccgct ccgcgagaac gcgacactgc
     3361 ggccgagcgc tccactgagg catcgcccgg cgcgagatcg ctcgcgcgca gcgccatcat
     3421 cgtcagtctg gcgttcgtgc tttcgcgggt gctggggctc gtccgcgagg tggtgctggc
     3481 aaaccagttc ggtaccacca gcgaaatgga cgcgtacgtg agtgccttcc gcatccccga
     3541 cctgctcttt ctcgtcgtga tcgcggggtc gttcggcgcg gcgttcattc cgatcttcgg
     3601 tgacttcctc acccgaggcg acgaggagcg cgcctggcgg ctcgcgtcgg cggtgctgac
     3661 atgggcgggt ctcgtgatgc tcgccctgtc gctgctcacc ttcgtgctcg cccggccgct
     3721 gatgatggtc gtcgcacccg gcttcgacga cgagacgacc gacctcgccg tcgagctgat
     3781 gcgcatcctg ctcctctctc ccatcttcct gggaaccggc atcgccttca agggcatcct
     3841 cgaggcacag catcagttca cgctgccagc cctcgcgccg ctggtctaca acgcctcgat
     3901 catcgttggc gctgtcgcct tcgcgccgac ctacggcatc cacgctgtgg gctgggcggt
     3961 gatcgctggt gccgtcggcc acatgctgat ccagctgccc ggcgtgctgc gctccggtct
     4021 tgtcttcaca ccgacgatca gccggcaggt cgaggggctg gtcgatgtgg cccggctgct
     4081 ggggccgcgc atcgtcgggc tagccgcttt tcaggtcaac ttcattgcca tcaacgcctt
     4141 cgcctcgacg acctcggacg agcacgtctc ggcactcaac tacgcttggc aactgctgat
     4201 gctgccccac ggcgtgctgg cgctgagcat ctcgacggtc atcttcccga cgctcgccgc
     4261 gctttacagc caaggcagga cggaggagtt ccgagcgacg ctcgatcgaa cccttcggcc
     4321 gctgctcttt ctcagcatcc ctgccgggat cggactctac ctcgtccgcg agccgatcgt
     4381 gcggctcatc taccagagcg gtaccttcga cgagaactcg accgatctgg tggcaacacc
     4441 gctcgccttc tttgcgctcg gcctggtcgg ctacgcgctc accgagatcc tgacgcgcgt
     4501 cttctacgcc acacgggata cccgcacccc ggtcgtcact ggcgtgctca ctgtggcgct
     4561 gaacctgctg ctcgcatcgc tgctgatcga ctcactgagt cacgcagggc tggcgctggc
     4621 actgagcgtg accaccggtg ccgaggcggt gatcctctgg ctcttcctgc gccagcgagc
     4681 gggtggcgtg gtgagcagta cgttcgcacc atggctgctc cgcgtgctgc tggcaaccac
     4741 cgtcatggcc ctgctgatct ggctgacgat cgagaccgtc ttggaggtgc agaccgacaa
     4801 ggagatcccg ctgctgatgc gcgaggcgtt cttcgtctac gcgctgggca tgtacatgct
     4861 ggcgttcgtc gtcgccgcgt ggctcctgcg catcccggag ctgctgcaga tcaccagcaa
     4921 agccgccgcc cgccttcccg ctcccctcag gggtcggctg cccggtcaca tcggccccta
     4981 ggaacagtcg acacgcgcgc gaacagggcc gtcccaccct ctccgctata cttgccggga
     5041 acgcagccag tcccgaaacc ggcatgtctt cgcgccccca accccttgac agaacggatg
     5101 ccagtccacc catgagcgag cagtcagcga catcccatat ccgcaatttc tgcatcatcg
     5161 cccacatcga ccacggcaag tcgacgctgg ccgatcggct gctgcagcgg acggagacgg
     5221 tcgaagaccg caagatgaag gagcagctgc tcgactcgat ggacctcgag cgcgagaagg
     5281 ggatcacaat caaggcgcgg gcggtccgca tggcctacac ggcgcaggat gggcagaagt
     5341 accagttcaa cctgatcgac acgccgggcc acgtcgattt ctcctacgag gtctcccgct
     5401 cgctcgctgc cagtgagggc gcgctggtgg tggtcgacgc ggcgcagggt attgaggcgc
     5461 agacgatggc caatgtctac ctcgcgctcg aacacgacct ggcgatgatt gccgtgatca
     5521 acaagatcga cctcccaaat gccgacccgg aacgcgtcgg caaggaggtc gccgacttca
     5581 tcggcctgct cggcgaggag atcatccccg cctccgccaa ggaggggacg ggtatcgacg
     5641 agatcctcgg agcgatcgtg cagacggttc cgccgcccga caacgagggg atcgacaagc
     5701 cgctgcgcgc gttgatcttc gactcccact atgatccgta caagggcgtg atcgcctatg
     5761 tgaagatcgt cgacggcaag atcgacgacc acgacgagat ccgcgtgatg ggcaccaaga
     5821 aggaggccgg tgttctcgag ctcggattct tcaacccgga tctgcaggcg accgcctcgc
     5881 tcaataccgg cgaggtaggc tacatcgcca ccgggctgaa ggaggtcaag gacgttcaag
     5941 tcggcgacac aatcacgctc gtagagaaac ccgccgagac gccgctgaag ggctaccggc
     6001 cggcccagcc gatggtattc gccggcctct accccaccga gagcgatgat ttcccggtgc
     6061 tgcgcgacgc gctcgaccgg cttcagctca acgatgcctc cctcacctac gagcccgagt
     6121 cgtcggcggc gctgggtttc ggcttccgat gcggtttcct cgggctgctg cacatggaaa
     6181 tcatccagga gcggctggag cgcgagtacg acatcgacct gctcgccacc gcgcccagcg
     6241 tgcagtacca cgtccgcatg ctgaacacgg gcgagatccg caagatccac aacccgtcgg
     6301 acatgccgcc gaccggcgag atcgacgcga tcctcgagcc atggatgcac ctcagcatca
     6361 tcgcaccgtc acgctacatc ggcacgatca tggagctgac cacaagtcgc cgggccaagt
     6421 tcatcgagat gatctacctc gatgaggagc gcgtgcagct gaagtacgat atcccgctcg
     6481 ctgaattgat cgtggatttc tacgacgcgc tcaagagccg tacccagggc tacgcctcgc
     6541 tggactactc gtatgccgag atgcgcgagg ggaacctggt caagttggac gtgttggtca
     6601 acggccaggc ggtcgacgcg ctctcgatca tcacccaccg tgacgatgcc tactacaagg
     6661 gtcgcgagct ggtgcagaag ctgcgcgggc tgattccgcg ccagatgttc gacgtgccga
     6721 tccaggcgtc gatcggcagc cgcgtgatct cccgtgaaac gatcaaggcc atgcgcaaga
     6781 acgtgctctc caagtgctac ggcggtgacg tgagccgcaa gcgcaagctg ctcgagaagc
     6841 agaaggaggg caaggcacgc atgaagatgg tcggcagcgt cgaaatcccg caggacgcgt
     6901 tcatggccgt gctgagcatt ggctcgtcct ccgacaagcc gcaatgaccg ccgacatcac
     6961 gatcgtcgga ctcggacccg gcgatcccgc tcggcgcacc atgcccgtcc aggaggcaat
     7021 cagcgacacc gagtggctct tcctgcgcac cacccaccat cccgggattg aggacctcgc
     7081 g
//
LOCUS       DASYWL010000319         6372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_261063, whole genome shotgun sequence.
ACCESSION   DASYWL010000319 DASYWL010000000
VERSION     DASYWL010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6372
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261063"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3035
                     /gene="mfd"
                     /locus_tag="VGR22_11220"
     CDS             <1..3035
                     /gene="mfd"
                     /locus_tag="VGR22_11220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEV2129179.1"
                     /translation="LVPGDRLDLSEFQDWLGRIGYLRVPMVQEPGQFAIRGGIIDLFP
                     TANELPVRLDLFDTDLESIRTFDVHSQRSRDRVERVTLLPTLDLPLWRLRDALPQLRG
                     IDVSMLRAEVRDDWEHQLEVIASRSTPGAPDLFAGYVSMTRDTLLRFLDGRSTVIVDQ
                     PEAVDLAASEWEQQARDLEVSLIRNGEMSPGITSPVTRWPIVRDDLARHPRLLLGESR
                     AGKTLQAGMLGNPPVYAGRLDDMAIDLQSRIADGWRITIATDQVQRVTELLEERDLHP
                     RRERKTRSNRPLPLGPGMIEVRPSGLDAGWMWADGKYGVWTDLELFGFRKRTRRTTKR
                     SMADHRAFALNLVEGEHVVHVDHGIARFAGLMRMETGGVEREYLLLVFDRGDKLYVPI
                     DQSDRVTRYSGGGQTPTLNRLGSGEWQRTKRRVRRAVREMAFELIELYARRGAGSGHA
                     FPADTQWDYELAEAFPWKETRDQTAAIDAVTSDMESTNPMDRLVCGDVGFGKTEIAMR
                     AAFKTVNDGKQAAVLVPTTVLALQHYNTFRERLAAFPVRIEMRSRLRSRNQQRRILDD
                     LEKGKVDIVIGTHRLVQKDVKFKDLGLVVIDEEQRFGVRHKEFLKQVRIDTDVLTLSA
                     TPIPRTLHLSLAGVRDISMIETAPQSRLPIRTFVAPFSEQLVREAILREMDRGGQVYV
                     VHNRVHDIDVLAERIRRIVPEARIGIGHGQMDEKVLEEVMLGFYRKEFDILLATTIIE
                     SGVDNPDVNTIIIDNADTLGLTQLYQLRGRVGRGTNRAYAYLLYQSHKSLRPEARERL
                     EVIQEANELGAGVRVAMRDLEIRGAGNMLGAEQSGHIGAVGYDLYMRLLSQAIEEVRS
                     GKPALESEPITLDLPLTALIPEDYVQDTELRLGLYRRIAAVETIDEVDELRNELADRF
                     GDPPVEVEHLLALIRLRIRATALGLDSLIEREREIILRPVDTSQLDVRRLERRLGDVV
                     KVTRNTVRLRLMRLGDRWQDGLDTVLDALEDVQFAQTA"
     gene            complement(3148..5574)
                     /locus_tag="VGR22_11225"
     CDS             complement(3148..5574)
                     /locus_tag="VGR22_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEV2129180.1"
                     /translation="MINTSVLRKLEFHEVVRRLSVHCQYNVAAELARELQPTPDRRVA
                     AQWVATTAEAVDLLGNFPDFTVGGARDIRELIVKAEKGARLQPSELLLVADTLHAARR
                     LKRSFLKLPEHEERFPNLLDLVGGIENVHQLESGLERSIGPRGDVLDTASTELQRLRK
                     AVRVAHSRVTERLQNLRSSGRVGAALQENIITVREGRYVIPVRAEARSVVKGIVHGTS
                     ASGQTIFIEPFDVVELNNAWRERQADEQEEANRILDGLSEQIAELADVLRRMVQAVAE
                     GDLALAKARYARQLDAARPRFHDTGAGDSRVRAEDAAHPTHRIRLIEARHPLLDQQTV
                     VPLSFEIGDTFRVLLITGPNTGGKTVALKTVGLLTLMAQVGMFIPAAASSELSVFPQI
                     FVDIGDEQSIEQSLSTFSGHMANIVSMLREVRSNSLVLLDELGAGTDPQEGSALARAL
                     ISRLLESRAMVVATTHYSEVKAYAYATPGVENASVEFDVETLSPTYRLTVGIPGRSNA
                     FAIARRLGIPSQVIEEARSILHAGDQQADELINDIRRRREQADEELAAAERTRADVEE
                     LRRRAARALRDAELTRREARDRATEEVERELQEAREIVRRLQRPIRQGSAPGPSPDEA
                     REATSKLTTALEETRRVRRRRQPTAPQPQQKRPLRRGDRVRVHSLDMPGEVISVDGDH
                     AVVQMGALKSRMPLGDLERTSGGGGDGEERQEAHQELPKPEFNVSVEADFRGLRAVEV
                     EERLEQYVHDAYLAGLPWVRIIHGKGTGALRKVVRDGLDAHPVVERSETAKQNEGGDG
                     ATVAYLRGNS"
     gene            5726..6364
                     /gene="lexA"
                     /locus_tag="VGR22_11230"
     CDS             5726..6364
                     /gene="lexA"
                     /locus_tag="VGR22_11230"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEV2129181.1"
                     /translation="MRKLSERQQAILDSISNFLEENDYPPTIRDIQLALNISSTSVVD
                     YNLKVLEERNYIRRNRNISRGIEIIGRSSARSGTVEVPVIGSIAAGQPIPVPGDLEGS
                     DVADVIELSPGLAPDNGTDLFALRVRGYSMIDALIGDGDIVVLKRQETCNNGDTVAVW
                     LEDEEETTLKVFYLEGSQVRLQPKNTAMDPIYTPAENVRIQGKLVSVLRSVQ"
BASE COUNT         1121 a         2097 c         2024 g         1130 t
ORIGIN      
        1 cgcttgtgcc gggagaccgt ctcgacctct cggagtttca ggactggctg ggccgcatcg
       61 gctatctccg ggtgccgatg gtgcaggagc cgggccagtt cgcgatccga ggcggcatca
      121 tcgacctctt cccgacggcg aacgagctgc ccgtcaggct cgatctcttc gatacggatc
      181 tcgaaagcat ccgcaccttt gacgtgcact cgcagcgatc acgagaccgc gtcgagcggg
      241 tcacgctgct gccgacgctc gatctaccgc tctggcgcct gagagatgct ctgccacagc
      301 tgcgcggcat cgacgtctcg atgcttcgcg ccgaggtgcg cgacgactgg gagcaccagc
      361 tggaggtgat cgcgagccgc agcactcccg gtgcgccgga tctcttcgcc ggctacgtca
      421 gtatgacgag agacacgctg ctgcgctttc tcgacggccg ctcgacggtt attgtcgacc
      481 agccagaggc tgtcgatctc gctgccagcg agtgggagca gcaggcgcgc gatttggagg
      541 tgtcgttgat ccgcaacgga gagatgtcgc caggaatcac ctctcccgtg acccgatggc
      601 cgattgtccg cgatgatctc gcgcggcatc ctcgactgtt gctcggggag agccgtgcgg
      661 gcaaaacgct gcaggcgggc atgcttggca acccgcccgt ctacgcgggc cggctcgacg
      721 atatggcaat cgacctgcag agccggatcg cggatggctg gcggatcacg atcgcaaccg
      781 accaggtgca gcgagtcacc gagttgctcg aggagcgaga cctccaccct cgtcgagagc
      841 ggaagacccg ttcgaatcgg ccgctgccgc tcggaccggg catgatcgag gttcgaccat
      901 ctggtctcga cgctggctgg atgtgggcgg atggcaagta cggggtctgg accgacctgg
      961 agctcttcgg gttccggaag cgcacgcggc ggaccacaaa gcggtcgatg gccgatcacc
     1021 gcgccttcgc gctcaacctt gtcgagggtg agcacgtggt tcacgtcgat cacggcatcg
     1081 ccaggttcgc gggcctgatg cggatggaga ccggcggcgt cgagcgcgag tacctgctcc
     1141 ttgtcttcga ccggggcgac aagctctacg tgccgattga ccagagcgac cgggtcaccc
     1201 gctacagcgg cggggggcag acaccgaccc tcaaccgctt gggatccggt gagtggcagc
     1261 ggacgaagcg gcgtgtccgt cgtgccgtcc gggagatggc gttcgagctg atcgaactct
     1321 atgcccgccg cggggccggc agtggccatg cctttccggc ggatacgcag tgggactacg
     1381 agcttgccga ggccttcccg tggaaggaga cgcgtgacca gacggcggca atcgacgcag
     1441 tgacctccga catggagtcg accaacccta tggatcggct cgtctgcggc gatgttggct
     1501 ttggcaagac cgaaatcgcc atgcgcgcgg cgttcaagac ggtcaatgac ggcaagcagg
     1561 ccgcggtatt ggtgccgacg accgtcctcg cgctgcagca ctacaacacc ttccgagaac
     1621 gtctcgcagc cttcccggtc cggatcgaaa tgcgctcgcg cctgcgctca cgcaatcagc
     1681 agcggagaat tctcgacgac ctggagaagg gcaaggtcga catcgtcatc ggcacgcacc
     1741 ggctagtgca aaaggacgtg aagttcaagg acctcggtct agtggtgatc gacgaggagc
     1801 agcggttcgg tgttcgtcac aaggagttcc tcaagcaggt tcgcattgat acggacgtgc
     1861 tcaccctctc ggcgacgccg atcccgcgca cgctccacct ctccctggcc ggtgtccgtg
     1921 atatcagcat gatcgagaca gcgccgcagt cacgcctgcc gatccgcaca ttcgtcgctc
     1981 cgttctccga acagctcgtc cgcgaggcga tcctccggga aatggaccgc ggcggacagg
     2041 tctacgtcgt tcataaccgg gttcacgaca tcgatgtgct ggccgagcgc atccgccgca
     2101 tcgttccgga ggcacgcatc ggcatcggtc acggccagat ggacgagaag gtgctcgagg
     2161 aggtgatgct gggcttctat cggaaggagt tcgacatcct gctggcgacg acgatcatcg
     2221 agtccggcgt tgacaacccg gatgtcaaca cgatcatcat cgacaacgcc gacacgctcg
     2281 gcctgacgca gctctaccag ctacgcggcc gcgtgggacg cggcacgaac cgcgcctatg
     2341 cctacctgct ctaccaatcg cacaagagcc tccgaccgga agcgcgagag cggctcgaag
     2401 tgattcagga ggcgaacgag cttggagcgg gcgtccgcgt cgccatgcgt gacctcgaga
     2461 tccgcggtgc cggcaacatg cttggcgccg agcagagcgg acacatcgga gcggtcgggt
     2521 acgacctcta catgcgccta ctcagccagg caatcgagga agtccgctcc gggaagcctg
     2581 cgctggagtc cgaaccgatc acgctcgacc tgccgctcac tgcgctcatc cctgaggact
     2641 acgtgcagga caccgagttg cgtctcggac tctaccgtcg gatcgcagct gtggagacga
     2701 ttgacgaggt cgacgagctg cgcaacgagc tggctgatcg cttcggcgat ccgccggtcg
     2761 aggtcgagca cctgctggcg ctgattcgac tgcggatacg ggcaacggcg ctcggcctcg
     2821 actccctgat cgagcgcgag cgagagatca tcctgcgccc ggtcgatacc tcccagctcg
     2881 acgttcggcg tctcgagcgc cgactcggtg atgtcgtaaa ggtgacgcgc aacacggtcc
     2941 gcctgcgact gatgcgccta ggcgaccggt ggcaggacgg gctcgatacc gtgcttgacg
     3001 cgctggagga cgtccaattc gcccagacgg cctgactcct caggcggaga ttccataccc
     3061 cgcaagactc ccgatggtgt cattcttgcc ggaggatact tcgcttgaga tgccactgcg
     3121 ttgcaccggt ccgggcaccc tgcaagccta tgagttgcct cgaaggtagg caacggtcgc
     3181 gccgtcgcca ccctcgttct gcttggcggt ttcgctgcgc tcgaccaccg gatgggcatc
     3241 gagtccatcg cgcacgacct tgcgcagcgc ccctgtccct tttccatgga taatgcgcac
     3301 ccacggcagc ccggcgaggt aggcgtcgtg gacgtactgc tcgagccgct cctcaacctc
     3361 cacggcacgg agaccacgaa aatcggcctc aaccgagacg ttgaattccg gcttcggcag
     3421 ctcctgatgt gcctcctggc gctcctcccc gtctccacca ccgcccgacg tgcgctccag
     3481 gtcgccgagt ggcatgcgcg acttcaatgc gcccatctgt accaccgcgt ggtcgccatc
     3541 gacggagatc acctcgcccg gcatatccag tgagtgcacg cgcacccggt caccccgtcg
     3601 gagcggccgc ttctgctgtg gctggggcgc agtcggctgt cgacgtcggc gcacacgtcg
     3661 cgtctcctca agtgccgtgg tgagcttcga cgtcgcctcc cgggcctcgt cgggcgaggg
     3721 gcctggcgcg ctgccctgcc ggatcggtcg ctgcaggcgt cggacaattt ctcgcgcctc
     3781 ctgcagctcg cgctcgacct cctcggtggc gcggtcccgc gcctcccttc gcgtcagctc
     3841 ggcgtctcga agcgctcgag cagcacgccg ccgcagctcc tcgacgtcgg cgcgggttcg
     3901 ctcggcggcg gcgagctcct cgtcggcctg ctccctgcgg cggcgaatgt cgttgatcag
     3961 ctcgtcggcc tgctggtcac cggcgtgaag gatcgatcgc gcctcctcga tcacttgaga
     4021 cgggatgccg agccgccggg cgatggcgaa agcgttggac ctgccgggga tcccgactgt
     4081 caggcggtag gtcggcgaaa gcgtttcgac gtcgaactcg acgctggcgt tttccacgcc
     4141 cggcgtcgca taggcgtagg ccttcacctc cgagtagtgg gtggtcgcga ccaccatcgc
     4201 ccgcgactcg agcaggcggc tgatgagtgc ccgcgccagc gccgatcctt cctgagggtc
     4261 agtaccagca ccgagctcat ccaaaagcac caggctgttc gagcggacct cccgcagcat
     4321 cgagacgatg ttcgccatgt gtccggaaaa cgtcgacagg ctctgttcaa tgctctgctc
     4381 atcgccgata tcgacgaaga tctgggggaa gacgctgagt tcggagctgg cggcggccgg
     4441 aataaacatg ccgacctgtg ccatgagcgt cagcaaaccg accgtcttca gggcaacggt
     4501 cttcccgccg gtgttcggcc cggtgatgag gagcacccga aaggtgtcgc caatctcgaa
     4561 ggagagcggc acgaccgtct gctgatcgag caatgggtgc cgtgcttcga tcagccgaat
     4621 gcggtgggtg ggatgagcgg cgtcctcggc gcgaacccgc gaatcgccag caccggtgtc
     4681 gtgaaagcgg ggacgagctg catcgagttg ccgcgcatac cttgccttcg cgagcgcgag
     4741 atcgccttca gcgaccgcct gcaccatccg tcggaggaca tcggccagtt cggcgatctg
     4801 ttcgctcaga ccgtcgagaa tccggttggc ctcctcttgc tcgtccgcct ggcgctcccg
     4861 ccacgcgttg ttgagttcga cgacgtcgaa cggttcgatg aaaatcgtct gtcccgacgc
     4921 cgatgtccca tggacgatcc ccttcacgac actgcgcgcc tcggctcgca ccgggatcac
     4981 atatcgaccc tctcggacag tgatgatgtt ctcctggaga gcagcgccga ctcgaccgct
     5041 ggagcgcagg ttctggaggc gttccgttac ccgcgagtgc gccacgcgaa ccgccttgcg
     5101 caatcgctgg agctccgtgc tggccgtgtc gagcacgtcc ccgcgcggac cgatcgatcg
     5161 ctccagccct gattcgagct ggtggacatt ctcgatgccg ccgacgagat cgagcaggtt
     5221 cgggaatcgc tcctcgtgct cgggcagctt gaggaacgac cgcttgagcc gccgtgccgc
     5281 gtggagcgtg tcggccacca gcagcaactc cgaaggttgc agtcgcgcgc ccttctcggc
     5341 cttcacaatt agctcacgga tatctcgcgc gcctcccacg gtgaagtctg ggaagttgcc
     5401 gagcagatcg acggcttcgg ctgtcgtcgc gacccactgg gcggcaaccc gccggtccgg
     5461 cgtcggctgc agctcacgag cgagctcggc ggcgacgttg tactggcagt gcacggagag
     5521 ccgccgcacc acttcgtgga actcaagttt tctaaggacg ctggtgttga tcaccgttcc
     5581 accgtttccc ctgtcacgca ccgtcgcaga cggcgcgcgt agccggattg acacgaacaa
     5641 gtgttcgaat tatactcgga gcacagcgtc gggtcaggca cctttcctac ccgcacccgc
     5701 cagactcgta ctcaaaggat ggcccatgcg caagctgtct gagcgccaac aagcgatact
     5761 cgactccatc agcaactttc ttgaagagaa tgactacccg cccaccatca gggatatcca
     5821 gctcgccttg aacatctcat cgaccagcgt ggtggactac aacctcaagg tgctcgaaga
     5881 gcggaactat atccgtcgca accgcaacat ctcgcggggc atcgagatca tcgggcggag
     5941 cagcgcccgg tccggcactg ttgaggtgcc ggtcatcggc tccatcgccg ccggtcagcc
     6001 gatccccgtt cccggcgacc tggagggatc cgatgtcgct gacgtcatcg agctcagccc
     6061 gggcctggct ccggacaacg gaaccgatct cttcgccctt cgcgtgcgcg ggtactcgat
     6121 gatcgacgcg ctcatcggcg acggcgacat cgtcgtgctc aaacggcagg agacctgcaa
     6181 caacggcgat acggttgccg tctggctcga ggacgaggaa gagacgacac tgaaggtatt
     6241 ctaccttgag ggctcgcagg tccggctcca gccgaagaac acggccatgg acccgatcta
     6301 cacaccggcg gagaatgtcc gaattcaggg taagctcgtg agtgtcctgc gatcggtgca
     6361 gtagaccatc cg
//
LOCUS       DASYWL010000320        10545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_410878, whole genome shotgun sequence.
ACCESSION   DASYWL010000320 DASYWL010000000
VERSION     DASYWL010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10545
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410878"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..248
                     /locus_tag="VGR22_11235"
     CDS             21..248
                     /locus_tag="VGR22_11235"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEV2129182.1"
                     /translation="MTHGTIKTLLNRGFGFITPDSDKNQDVFFHSSTVADDGFERLRE
                     GMRVSYQQEADPRDASRQRAAQVTPLTGADE"
     gene            719..1279
                     /locus_tag="VGR22_11240"
     CDS             719..1279
                     /locus_tag="VGR22_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129183.1"
                     /translation="MIAPDTELGLLLTAMGEYQDQQHASTGDHTYPTMVCADWAFHEA
                     RTGLGRERICRLLPQLVGDGLARGLGAGMVWGYRVSDGYAWIAERGQLQRSQWRQPLL
                     APVSALGPEAEDQLRHLLESARASGVTTQEIGRLEALFSDIAEAPGLDGAERDANLGQ
                     VLREHAGLSAFMVHGVLPFLRHVVAA"
     gene            1310..1534
                     /locus_tag="VGR22_11245"
     CDS             1310..1534
                     /locus_tag="VGR22_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129184.1"
                     /translation="MDGATCQLVDRCEAQASEPVQESIARLLAVYDDASALQRRILYE
                     RALYLQHGLLLEAVRFDPDARQQLQLRPDP"
     gene            1735..1983
                     /locus_tag="VGR22_11250"
     CDS             1735..1983
                     /locus_tag="VGR22_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129185.1"
                     /translation="MSDWWWYAFLVVVIVASVYLVLRIRRSRAPRSDTDATSRDYTGD
                     REAHRVGEMSDEDREWEAASLRRSHEAEERDRTPPGRA"
     gene            2103..2354
                     /locus_tag="VGR22_11255"
     CDS             2103..2354
                     /locus_tag="VGR22_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEV2129186.1"
                     /translation="MQRWEYAIITMDARGNMFWVGYSAPVEVDTSDYPLSDFGTVLGE
                     LGSKGWELVSVTQSANTSGGLDEKLYFKRPHEDRRNLKH"
     gene            complement(2478..2708)
                     /locus_tag="VGR22_11260"
     CDS             complement(2478..2708)
                     /locus_tag="VGR22_11260"
                     /inference="COORDINATES: protein motif:HMM:NF041374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
                     /protein_id="HEV2129187.1"
                     /translation="MDVTCSATLTCPECGAQHRETMHDGACQFFYRCDQCGMTLRPVE
                     GDCCVFCSYADVPCPPIQRERAATAASEHSPT"
     gene            complement(3018..3953)
                     /locus_tag="VGR22_11265"
     CDS             complement(3018..3953)
                     /locus_tag="VGR22_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129188.1"
                     /translation="MRRVLCAFLMLAVLIGMSGAAVAAQDGTPAVTDPNLLADLGYPE
                     LRVTTDGTTNDFPTEVEAGRYHVVLDNQSDQMEIDLEFWQLPEGVTVDDVMAFFEEAQ
                     ETGDFGVPDFFFDMTFNGGPTTSPGQSDSVVLDLTPGEWYVNVYSYNPETDESTDTVA
                     TVTVTGEMPELPAPEADVTVELVDFDFAVSGSVSSGPQIWEVTGAGSQPHHLVLSRVP
                     EGTTEEDVIELAGTFFVPPPDPSATPAATPVIQPALAFEDVEEVFYTLIFTQGQYNLY
                     DLTLEPGTYAMICFLPAPDGTPHVLMGMVEIITVE"
     gene            4932..5351
                     /locus_tag="VGR22_11270"
     CDS             4932..5351
                     /locus_tag="VGR22_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129189.1"
                     /translation="MGRILVPVTIVVAMAAFAVAALTAGFSPYQPGWWTAAVRLAVLG
                     GIVPMIFAVTIRIVPVFSPRRWRSEARLRLQVAFALAGVWTTYVSQPPVQISAWYSAM
                     GSPSPEESPSRSISRGSFASRWGRPRRCRFPGLVRPP"
     gene            5300..6190
                     /locus_tag="VGR22_11275"
     CDS             5300..6190
                     /locus_tag="VGR22_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129190.1"
                     /translation="MGKTPALPLPWPGQAAIERLATGFTRLAGVYLLVGLAVGFVTSW
                     WRSTSGRWDLVWAHALLVGFFLSMAAGVCYHVLARWTGRRWHWPIRLHLMVVTVGLPF
                     MLIALATGWMPLFVIAGPLQAAGIALLLVNIAPMLGGLPALTRIAMASASACLAVGVG
                     LGAVFAVEPAVGARLRFVHVELNLFGWTGLLICGVSYYLVPRLLGRPLRWPRLAAVQI
                     ACLAIGTLLSTGFLAWRVSGGGSPKLVVSTHALVALGFVLFGTLIAFTIQGERAPGVV
                     VAVPLQSRRGSSAPSGANAG"
     gene            complement(6277..7239)
                     /locus_tag="VGR22_11280"
     CDS             complement(6277..7239)
                     /locus_tag="VGR22_11280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEV2129191.1"
                     /translation="MVQLGYALSSEEFRPNELVDNAVQAEETGFPYALISDHFHPWMD
                     SQGQSPFVWSTIGGIAQRTSQLRLGTGVTAPIIRIHPAIIAQAAATVADMMPGRFFLG
                     VGTGELLNEHITGEHWPPISIRQEMLAEAVEIIRQLWQGGYQTIDGDYYTVENARLYT
                     LPETLPDICVAASGPESATLADRIGDGLVCTSPQAETVQAFNGARGKGKPTYGQMTVC
                     WAKTEDEGLETALKYWAYTGLPGQLSQELALPQYFQEAAQLVTKETIGKSVVAGPDAE
                     KYHQKIQSYIDAGFTHVYLHQIGPDQAGFFDFARRELLPKYQGA"
     gene            7436..7945
                     /locus_tag="VGR22_11285"
     CDS             7436..7945
                     /locus_tag="VGR22_11285"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEV2129192.1"
                     /translation="MEDVATPQGLLAVEEYLELEASSAITHEYVGGMLYALAGDSDRH
                     NRIAGNTYIALRAAARRTRCRVYMSDMRLRVGANFYYPDVMVACEEPETDNPTFRNDA
                     CVVVEVLSVSTESIDRREKLLAYRELPTLQAYLIVHQDIRRVERFFHGEDAVWQRADR
                     VKVPCRSRA"
     gene            7987..8406
                     /locus_tag="VGR22_11290"
     CDS             7987..8406
                     /locus_tag="VGR22_11290"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEV2129193.1"
                     /translation="MAEALAPHPAVSVETYLELEKDADVRHDECVGGRLYAMTGTSRR
                     HNTIAFNIARKLADAAEGTPCRVYMSDVKVRTPDDAFYYPDVIAACEPEPDDPYVEHQ
                     PCLLVEVVSPSTESIDRREKLAAYKKIPPVKSYLIVD"
     gene            complement(8565..9506)
                     /locus_tag="VGR22_11295"
     CDS             complement(8565..9506)
                     /locus_tag="VGR22_11295"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV2129194.1"
                     /translation="MEMRRVGNSGLLVSAIGLGGNTFGATIDGEDAVRTIQHAIDQGI
                     TFIDTADVYSMGCSEELVGQAIAGRRETVVVATKCGIPLDSGPHAGGLSRRWVTQAVE
                     DSLRRLGTDYIDLFYAHRPDPSTPIEETLSAMDDLVRAGKVCYIGCSNFRAWEVAHSM
                     GSSRLQRLEHWMAVQNRWNLLDGLDSPELEQAAETLGLGIIPYTPLASGLLTGKYHPG
                     VEPPAGTRFGDLERIRERMTEDKLRRAERVKAWAEERGHTPAEAAIAWLLAHPVVPSV
                     IAGARSPEQIDANIKAAAWTMTPEERDELRAVSAASD"
     gene            complement(9579..9989)
                     /locus_tag="VGR22_11300"
     CDS             complement(9579..9989)
                     /locus_tag="VGR22_11300"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV2129195.1"
                     /translation="MSTATPVCTVVRPREQFDGKQGLTYVEGISAERGGSTGICMHLL
                     TLPPGARAKAHLHEAHETAIYIISGESEMWYGEGLKEHLTVRAGELLYIPANTPHLPM
                     NTSDEPCVAVLARTDPNEQESVVLLPALEAAVPR"
     gene            complement(10114..>10545)
                     /locus_tag="VGR22_11305"
     CDS             complement(10114..>10545)
                     /locus_tag="VGR22_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129196.1"
                     /translation="VVDTMDFANVRPQVHRYIARGLTEDTEGNATGIGMFDFALQRLV
                     DEMDPVPTYMNMITAKSPQGARVPITVENDRQALTLAVASGIKVDAPNARIMRIQSTK
                     HLEEMHVSAPVLEELRASGVPVEVLEDVHPIAFDADGMFAD"
BASE COUNT         1798 a         3487 c         3372 g         1888 t
ORIGIN      
        1 cgcgaaggat atgtacctct atgacacatg gaaccatcaa gactctgctc aaccgaggct
       61 tcggcttcat cacccccgac agcgacaaga accaggatgt gttcttccac agctccacgg
      121 tcgccgacga cgggttcgaa cgcctccgcg aggggatgcg cgtttcctat cagcaggaag
      181 ccgacccacg tgatgcctct cggcagcggg ctgcgcaggt cacgccgctc accggcgccg
      241 acgagtagca catcggcacg atcggcgcac ctgtcgcgat cgtcggaatc gtcaggagtg
      301 ttccgggccg acgcccggaa cctcttgaag ccgtcgtctc tggatgtgca gccacacgat
      361 cgtcgggcgt cggcctgttc atgacggaaa ggtcgcccca tgacgacatg tttcggccat
      421 atgagcgcgc tacgccgcga acggtcagcg gaccgtccac gttgccaacc ataccgcgcg
      481 ccggcggttc agccgtcggc gcggccgacc cccacgagat cgcgcctgcc gctggccatc
      541 acccgcatgc tcggggccga atgtgccctc tgcggaggag tcggctgcac gccgtgcgcc
      601 gattcgggcc ttcgatgagc cccgctgcgg gcatcgggtg ccgccttcgc gttcatcgct
      661 cgaacgtcca cggccgcgat gcggttgacc gggccaacat cacgcggtgg ggcaaccgat
      721 gatcgcgcct gacacggagc tcggtctcct gttgaccgca atgggcgaat atcaggatca
      781 gcaacatgcc agcacgggcg accacacgta cccaaccatg gtgtgtgccg actgggcctt
      841 ccacgaggcc cgcacggggc tcggtcgcga gcgaatctgc aggctcctgc cgcagcttgt
      901 cggcgacggc ctcgcacgcg ggctgggagc cgggatggtt tggggctatc gcgtgagcga
      961 tggctacgca tggatagcgg agcgcggcca gctgcagcgt tcgcagtggc ggcaacccct
     1021 gctggcgccc gtctccgcgc tcggcccgga ggccgaagac cagctgcggc acctgctcga
     1081 atcagcgcgt gcatcgggtg tgaccacgca ggaaatcggt cgcctcgagg cgctcttcag
     1141 cgatatcgcg gaggcgcccg ggctggacgg cgccgaacgg gatgccaacc tcggacaggt
     1201 tcttcgggaa catgcggggc tttccgcctt catggttcat ggtgttcttc cgttcttgcg
     1261 acatgtggtg gctgcctgag gcgtcatggc ggaatgatgg agacggggca tggatggagc
     1321 aacatgccag cttgtggacc gatgcgaggc gcaggcgagc gagcctgttc aggagtcgat
     1381 tgccaggctg ctggcggtct acgacgacgc ctcggctctc cagcgacgaa tcctgtatga
     1441 gcgggcgctc tacctgcaac acggtctgct gctcgaagcc gttcgcttcg atcctgatgc
     1501 tcgtcagcag cttcagctgc ggcccgatcc gtaggatggc ggccatcgca cgctgggcaa
     1561 acgggttcgt gatcccggtc tgccgatgcg tcgatagata ctgacggtcg cggtcaatga
     1621 tcatcaacgg aagcccattg acacattaag gagcggtacg tccgtcaccg agtgggtgag
     1681 ggcccgggat cacgcccgga gaacacgggc cgatcgatga aacgaggtcg aaccatgtcg
     1741 gactggtggt ggtacgcatt cctggtcgtg gtgattgtcg catcggtgta tctcgtcctc
     1801 cgcattcgtc gctcgcgcgc tcctcggagc gacacggatg ctacgtcgcg cgactacacc
     1861 ggcgaccgcg aggcccatcg ggtcggcgag atgagcgatg aggatcgcga atgggaggcg
     1921 gccagcctca gacggagcca cgaggccgag gagcgagatc ggacgccgcc gggccgggca
     1981 tgaccacgcc tccggggagc cgtaacggct gtacggagaa acgccgatag ccgtggcgcc
     2041 ggctggaccc cgaacggggt ccagccatat tgtgtgggaa catttggaaa ggcagtacca
     2101 tcgtgcagcg ctgggaatac gcgatcatta ctatggacgc tcgcgggaac atgttctggg
     2161 tcggctactc cgctccggtt gaggttgaca cgtcggacta cccgttgagc gacttcggca
     2221 cggtcctcgg cgaactcggg tccaagggct gggagctcgt cagtgtcacg cagagtgcca
     2281 acaccagtgg tggacttgac gagaaactct acttcaagcg cccccatgag gacaggcgaa
     2341 acctcaagca ctgactgctt ttcaaccccg gcctccgccg ctcgacgttg ggcacaaacg
     2401 gcgtgccgtg gtcgcggagg atatcaccct cgggcggctg ctggcgatga acaagcagcg
     2461 ggcggggcac tgacgcatta cgttggcgag tgctcgctcg cagcggttgc agcacgctcc
     2521 cgctggatcg gcggacatgg cacgtccgcg tagctgcaga acacgcagca gtccccctcc
     2581 accgggcgca gcgtcatccc acactggtca caccgataga agaactgaca ggcgccatca
     2641 tgcatggtct cgcgatgctg cgcgccgcac tcgggacagg tgagcgttgc agaacaggta
     2701 acatccataa ggtcctccca gcagggtgga tcgcatcgat cctagcgtgg ttgtcaggca
     2761 ccgtggtgac accgccatcg acgggacggt atgggacgcc gacaactggc cgaaggggga
     2821 ggtcccgaga ggtcgaggag gatgtcatca tcggccggtt gttggcacaa aaccaggagt
     2881 aggccaaacg gcaggagagg acacaaagcc ctcccctgcc cggtgtttgc gacatccaac
     2941 gcacgaaccc cgcgccgtca atgcaccggg ttcgcggtcg gggtcgacca atcgtttccg
     3001 agatcaatcc accgccgtta ctcgacggtg atgatctcga ccatgcccat cagcacgtgc
     3061 ggcgtgccgt ccggcgctgg caggaagcag atcatcgcgt aggtccccgg ctcgagggtg
     3121 aggtcgtaca ggttgtactg cccctgtgta aagatcaggg tgtagaagac ctcctcgaca
     3181 tcctcgaagg ccagggccgg ctggatgacc ggtgtggcag cgggcgtcgc gctcgggtcc
     3241 ggaggtggca cgaagaaggt gccggccaac tcaatgacat cctcctcggt ggtcccctcc
     3301 ggcacgcggg agaggacgag gtggtgcggc tgggatcccg cgccggtcac ctcccagatc
     3361 tgcggcccag agctgacgga gccggagacc gcgaagtcga aatcgaccag ctcgacggtg
     3421 acgtccgctt ccggagccgg aagctccggc atctccccgg tgacggtgac ggtggccacc
     3481 gtgtcggtgg actcgtcggt ctcggggttg tagctgtaga cgttgacgta ccactcgcct
     3541 ggcgtcagat ctaggacgac gctgtcgctc tggccggggg aggtggtggg gccgccgttg
     3601 aaggtcatgt cgaagaagaa gtcaggtact ccgaagtccc cggtctcctg ggcttcctcg
     3661 aaaaaggcca tcacatcgtc gacggtcacc ccctcgggca gctgccagaa ctccaggtcg
     3721 atctccatct ggtcgctctg gttgtcgagc acgacgtggt agcgcccggc ctccacctca
     3781 gtgggaaagt cattggtcgt gccatcggtc gtgacccgga gctccgggta gccgaggtcg
     3841 gccagcaggt tcgggtcggt cacagcgggg gtgccgtcct gggccgccac cgcagcaccg
     3901 gacatgccga tgagaacagc cagcatcaaa aaagcgcaga gaacgcgtcg catattcttt
     3961 ctcctccgca gacaagagtc gacaacgatc gaaaacgaat ggctgagccg aggcagttgt
     4021 accacaccca aatgccgcga tccggggtgc catcccgtgc tggcgccggg cctccaatcc
     4081 tacattatgt acatgttact cattatagat gacacaatag gaagagtcaa tacatcatga
     4141 cagaagatca ttcttcgaag cacagcgcac ccatccggcc gatctttgga gaggagctgt
     4201 ctcaagcaac ggcatggcaa catccagttg agcccaattc ttggggcctg tacgtctgtt
     4261 ccggcccgtg aggcgaacgc gacagccggc acatccggcc tgtccgacac cacggcacag
     4321 gaacctggcc ggcgagaccg ggaccatcgg ccgtggtggc tgcatgttgc cggtccccga
     4381 cgccccgcca atcaacagca cccgactagt cgttcatccc tcctcgatat gagattgtca
     4441 tgtttttgat acccctgccc tcccgctgca aggctcctgt aacgaacagt ccggtaggct
     4501 acgggccagc tcgcctgtgt gtggatcggg agcggagagg acgcccggct ccgggcatcg
     4561 catgccgttg gtcatcatgc aggacagccc accgaagatg gctatcagcg agcacggcca
     4621 gcgcagcggg tacgacccgc accgccaatc cacgcgcgcg ccgttcctcg gccactgttg
     4681 atcgaaagga aaagagagat gacaaggatg acaccgccat cgccccctcg cgacatcgcc
     4741 gaccggctga tccgggatct cgtcgatgag caccccgacg tcatgaccgt gctcgcgcca
     4801 tacggcctcg acctctgctg tggcgggggc acccgctcgg cgaggccctg gacctccacg
     4861 gcatcccaca cgagccagtg ctcagcgagg tcgcccggat cgtcgacggc cggcccaaga
     4921 aatcctgagc gatgggtcgt atcctggttc ccgtaaccat cgtcgtcgcc atggccgcat
     4981 tcgccgtggc ggcgctcacc gctggcttct ctccgtacca gccgggctgg tggacggctg
     5041 ccgtgcggct cgcggtcctc ggcggtatcg tgccgatgat tttcgcggtc acgatccgga
     5101 tcgttccggt cttctccccg cgaagatggc ggagcgaggc gcggttacgg cttcaggtcg
     5161 cgttcgcgct cgccggtgtc tggaccacct acgtgagcca gccgccggtt cagatatcgg
     5221 cctggtactc ggccatgggc tcgccctcgc cggaggaatc gccttcacgg tcaatatcgc
     5281 gcggctcttt cgccagccgg tggggaagac cccggcgctg ccgcttccct ggcctggtca
     5341 ggccgccata gagcgcctcg caaccggctt cacccgcttg gcgggcgtgt acctgctcgt
     5401 ggggctcgcc gtcgggttcg tgacaagctg gtggcgatca acgagcgggc gctgggatct
     5461 cgtctgggcc cacgcgctgc tagtcggatt cttcctctcg atggcggcag gggtgtgtta
     5521 ccacgtgctg gcccgctgga ccggccggcg ctggcactgg ccaatccgcc tccacctcat
     5581 ggtggtgacg gtcggcctgc cattcatgct gatcgcgctc gcgactgggt ggatgcccct
     5641 gttcgtcatc gcgggaccgt tgcaggcggc tggcatcgcg ctcctgctgg tcaacatcgc
     5701 gccgatgctt ggcggcctgc cggcactcac gaggatcgcg atggcgagcg cgagtgcctg
     5761 cctggcggtc ggtgttggcc tcggggccgt cttcgcggtc gagcctgcgg ttggggcgcg
     5821 gctccggttc gttcacgtgg aactcaacct cttcggatgg accggactcc tcatctgcgg
     5881 ggtcagctac tatctggttc cccgtttgct cggccgccca ctgcggtggc cacgactcgc
     5941 ggctgtccag atcgcgtgcc tcgccatcgg aacgctcctc agcacagggt tcctggcatg
     6001 gcgcgtctcc ggcggcggct cgcccaagct cgtcgtgagc acccacgccc tggttgcact
     6061 cgggttcgtg ctcttcggca cgctgatcgc ctttaccatc caaggcgaac gcgcaccggg
     6121 agtcgtcgtg gcggtgccac tgcaatcgag gcgtggttcc agtgccccgt ccggcgcgaa
     6181 cgccgggtag tcagctgacg cagcaatctg atcgtgaggg ccgggaggtc gcaggcatct
     6241 cccgggcctc acggttcgtg catcggcaat gccgacctac gctccctggt acttcggcag
     6301 cagctcccgc ctggcgaagt cgaagaaccc tgcctggtcc gggccaatct ggtgcagata
     6361 gacgtgcgtg aacccggcgt cgatgtagga ctggatcttc tggtggtact tctcggcgtc
     6421 cggccctgct accactgact tgccgatcgt ctccttcgtc accagttgcg ccgcctcctg
     6481 gaagtactgc ggcaacgcca gctcctgcga gagctggccg ggcagccccg tatacgccca
     6541 atacttcagc gccgtctcga gcccctcgtc ctcggtcttc gcccagcaga cagtcatctg
     6601 cccgtaggtc ggcttgccct tgccgcgggc gccgttgaac gcctgcaccg tctccgcctg
     6661 cggcgatgtg cacacgagac catctccgat cctgtcggcg agcgtcgccg actccggccc
     6721 cgacgcggcg acgcagatgt cgggcagcgt ctcggggagc gtatagagcc gagcgttctc
     6781 gaccgtataa tagtcgccgt cgatggtctg atacccgccc tgccagagct ggcggatgat
     6841 ctcgaccgcc tcggcgagca tctcctgccg gatcgagatc ggcggccagt gctcgccggt
     6901 gatgtgctcg ttgaggagct caccggtgcc gacaccgagg aagaagcgtc ccggcatcat
     6961 gtccgctacg gtagctgccg cctgggcgat gatcgccgga tggatgcgga tgattggcgc
     7021 cgtgacgccg gtgccgagcc ggagctggct ggttcgctgc gcgatgccgc cgatcgtgct
     7081 ccagacgaac gggctctgcc cctgcgagtc catccacgga tggaagtggt ccgagatcag
     7141 cgcatacgga aagccggtct cctcggcctg gacggcgttg tcgaccaact cgttcgggcg
     7201 aaactcctcg ctggaaagcg catatccgag ctgaaccatg gtggtaccca gtctcctgtg
     7261 tggggcgaat cgaatcgtcg cgggccgcat cggtcccgcg tgtccctctc cacagatcac
     7321 gttgcatgcc acgagacgtg ccggggccgg tctcgacacc gcgagttccg accagcttcc
     7381 gatccatggc gtccgctcgg acgggggttg ccgggactgc ctatatactg cccgtatgga
     7441 agatgtcgcc acgccgcagg gtctcctcgc ggtcgaggag tacctggagc tcgaggcaag
     7501 ctccgcaatc acgcacgagt acgtcggggg catgctgtac gcccttgccg gcgacagcga
     7561 ccggcacaat cggatcgccg gcaacaccta catcgcgctt cgtgcggccg cgaggcgcac
     7621 gcgttgtcgc gtctacatga gcgacatgcg gcttcgcgtc ggtgccaatt tctactatcc
     7681 ggatgtgatg gtcgcctgcg aggagccgga aacggacaac ccgaccttcc gcaacgacgc
     7741 ctgtgtggtt gtcgaagtgc tctcggtgag cacggaatcg attgatcggc gcgagaagct
     7801 tctcgcctac cgggaactcc cgaccctgca ggcgtacctc atcgttcacc aggacatccg
     7861 tcgcgtggag cgcttctttc acggcgagga tgcggtctgg cagcgcgccg accgtgtgaa
     7921 ggtgccgtgc cgatcccgtg cctgaacacg aaggtggcaa tggcagacat ctacgagggg
     7981 ctgtgaatgg ccgaagcact cgcaccgcac ccagccgtga gcgtcgagac ctacctcgag
     8041 ctcgagaaag atgctgacgt ccgccacgac gaatgcgtag gtggcagatt gtacgcaatg
     8101 actggcacct caaggcgcca taacaccatt gcgttcaaca tcgctcgaaa gctcgccgat
     8161 gccgcagagg ggacgccgtg ccgcgtctac atgagcgacg tcaaggtgcg gacgccggac
     8221 gatgcctttt actaccctga tgtcatcgcc gcctgcgagc ccgagcccga tgacccctac
     8281 gtcgagcacc agccctgcct gctcgtcgag gtcgtctcac ccagcaccga gtcgatcgac
     8341 cggcgggaga aacttgcggc gtacaagaag atcccgccgg tgaagtcgta cctgatcgtc
     8401 gattaggacc ggcaacgggt cgagcaccac ttccgtctcg agagcggcga gtggatacag
     8461 gccgatttca tcggcgaggg cgagatccgc accccctgcc ctgttacctt actgaccctg
     8521 gcacggattt acgccggtct ctagcccacg gtcacgccgg caggtcaatc gctggccgcc
     8581 gacaccgccc tcaactcgtc gcgctcctct ggggtcatcg tccacgccgc cgccttgatg
     8641 ttcgcgtcga tctgctccgg ggatctcgcg cccgcaatca ccgacggaac cactgggtgc
     8701 gccagcagcc atgcgatcgc cgcctccgcc ggggtgtggc cgcgttcctc cgcccacgcc
     8761 tttacccgct cggcacggcg cagcttgtct tcggtcatcc gctcgcgaat gcgctccaga
     8821 tcgccgaacc gcgttccggc tggcggctct accccgggat gatacttgcc ggtgagcagg
     8881 cctgacgcca acggggtgta cgggatgatg ccgagcccca gtgtctccgc cgcctgctcc
     8941 agctcagggc tgtcgagccc gtcgagcaga ttccaccggt tctgcaccgc catccagtgc
     9001 tccaggcgct gcagccgact gctccccatg ctgtgggcca cctcccacgc cctgaagttg
     9061 gagcagccga tgtagcatac cttcccggcg cgaacgaggt cgtccatcgc gctcagcgtc
     9121 tcctcaattg gcgtgctggg atcgggtcga tgcgcataga agagatcgat gtagtcggta
     9181 ccaagccgcc gcagcgagtc ctccaccgcc tgggttaccc agcggcgcga cagcccgccg
     9241 gcatgcggcc cgctgtccag tgggatgcca cacttggtcg ccaccacgac cgtctcccga
     9301 cgtccggcga ttgcctgtcc gaccaactct tccgagcaac ccatcgagta gacatcggcg
     9361 gtgtcgatga aggtgatgcc ttggtcgatc gcatgctgga tcgtccgcac ggcgtcctcg
     9421 ccatcgatcg tcgcaccgaa ggtgttgccg ccgagcccga tcgccgaaac cagcaggccg
     9481 ctgttgccga ctcgccgcat ttccatggaa aacccttctc ttggcaccag ccgctgcagt
     9541 cgacgccagc gtgcaggccc gatccggtcg atgtccggct atcggggcac agccgcctcc
     9601 agcgcgggca gcaacaccac gctctcctgc tcgttcggat cggtgcgggc cagcacggca
     9661 acacacggct cgtccgacgt gttcatcggc aggtgcggcg tgttggctgg aatgtaaagc
     9721 agctccccgg cccggacggt caggtgctcc ttcaacccct cgccgtacca catctcggac
     9781 tcgccgctga tgatatagat cgccgtctcg tgcgcctcgt gcaggtgcgc cttcgcccga
     9841 gcgcccggtg gcagggtcag caggtgcatg cagatgcccg tcgagccgcc gcgttcggcg
     9901 gagatgcctt ccacataggt cagtccctgc ttgccgtcga actgctccct cggacgtacc
     9961 acggtgcaga ctggggtggc ggtggacacg gacactcctt atcaattcaa gagccaagtt
    10021 aagagccagg tgaccggggc gaagccgcaa ttgcccgcag gggtgatctc ggcgccagtc
    10081 cgtccgcccc tccggattgg ccagcagtcc tcgtcagtcg gcgaacatgc cgtcggcgtc
    10141 gaacgcgatc ggatgaacgt cctccagcac ctcgactggc acgccgctcg ccctcagctc
    10201 ctccagcacc ggcgcggaga catgcatctc ctccagatgc ttggtgctct ggatgcgcat
    10261 gatcctggcg ttgggcgcgt cgaccttgat gccggatgcc accgcaagcg tgagcgcctg
    10321 ccggtcgttc tcgaccgtga tcggcacccg cgcgccctgc ggcgacttcg cggtgatcat
    10381 gttcatgtag gtcggcaccg ggtccatttc gtcgaccagc cgctgaaggg caaagtcgaa
    10441 catgccgatg ccggtggcat tgccctcggt gtcctcggtc aggccccgcg cgatgtagcg
    10501 gtgaacctgg ggccgtacgt tcgcgaagtc catcgtgtcg acgac
//
LOCUS       DASYWL010000321        10559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_223709, whole genome shotgun sequence.
ACCESSION   DASYWL010000321 DASYWL010000000
VERSION     DASYWL010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10559
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223709"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="VGR22_11310"
     CDS             complement(<1..1028)
                     /locus_tag="VGR22_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEV2129197.1"
                     /translation="MPTTITDLVALDIRFPTSKTFDGSDAMNPDPDYSAAYVILQTDH
                     PLGLEGHGLTFTIGRGNELCVAAIEALAPLVRGKILESFTADMGAFWQTITGDSQLRW
                     VGPEKGVIHLATAAVVNAVWDLWAKTEGKPLWKLLADLPPERLVACVPFRYITDAITP
                     EEALAILHNNAPTKADREAQLRRDGYPAYTTSAGWLGYSDEKLRRLCREGVTSGWSHF
                     KVKVGRDIEDNLRRAAIIREEIGPHRRLMMDANQVWDVGEAVAQMRQLARFDPWWIEE
                     PTSPDDILGHATIARAVAPIGVATGEHCHNRVMFKQLLQAEAIRFCQLDACRLGGVNE
                     VLAVLLLAA"
     gene            complement(1276..1351)
                     /locus_tag="VGR22_11315"
     tRNA            complement(1276..1351)
                     /locus_tag="VGR22_11315"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1316..1318),aa:Arg,seq:tct)
     gene            1527..3953
                     /locus_tag="VGR22_11320"
     CDS             1527..3953
                     /locus_tag="VGR22_11320"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2129198.1"
                     /translation="MSIEPDVALPVQPNRLIGREAICAHVGDLVLAEHHRLVTLIGPG
                     GVGKTRLAMCLAEDLAANFPDGTWFVPLEHLRTSELVMPAIARTMGVHEALGQVGLDL
                     LITTLRDRRALLVLDNLEQVAGAASEIAQLLAGCKYLRILGTSRASLRLRAEREVPVE
                     PLDTPAAGSVVAAADIAESAAVELFVERARAVRPTFNLSNFTAPIIAEICRRLDGLPL
                     AIELAASTINVLSPQELLDRLNNRLQLLSNGAVDLPARQRSLRSAIAWSHDLLDANER
                     RLFESLSVFAGEFGIEAAAVLLDENDETGGDSDVLPTLSSLVDKSLLNRIDVPDGEVR
                     FRMLSTIKEFGLERLADSGDLVVAQHRLVAWALDLAERAAPELTGPEQARWLSILERE
                     HDNLRAALIVARDVSPEEGLRVASALWRFWATRGYLTEGCSWLEQYVAASDGIEDRYL
                     ARALVSLGNLALDLGAYEDAIVSYDHGLRLFEKLGDQWGVADALNGTGLVDWYRGENL
                     KARDAHEQSLAIRRELGNRQGEANSLSNLANAVRDLGEIELAGELHRQALQIRMSRGD
                     RGATGFSLVNLGDISWRLGRADEAQSFFQQSLDTFREVHDMLGEAYALLGIGLAAAMA
                     NDAGEARRSIADSLAIRLRLGDRRGVIECVEGIAALAARGRQESKAARLFGAAQALRR
                     QIDAPLAEADRERQARLVAKLRPALADSAFRQEWDAGAALTMADAAEEALKAAVASGA
                     PREWPGGLSQREVEVLRLVAAGLTNGEVADRLFLSRRTVDAHMRRIYDKLSLASRLEA
                     IRYATQHGLA"
     gene            4098..5279
                     /locus_tag="VGR22_11325"
     CDS             4098..5279
                     /locus_tag="VGR22_11325"
                     /inference="COORDINATES: protein motif:HMM:NF039815.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129199.1"
                     /translation="MMQFVPGSMIVTLVLLAFAGFGMPTAAAQPAPALMSINSWLCRS
                     DYDRISDCEKIGGVIVSIRSDGQVVGEVTTVADSAVEIEVLPGQSVTASIVGGQPEGT
                     TLKNAGLSFTAAERQNPITLVFLERSGSGSIDSDSDGLSDEEEAAYRTGPDVADSDGD
                     GIQDGGEINIGADPLVADTDGDGFLDGEEFERATDPLDSSSFPVETEPNSIVISAFTC
                     PAGHGGKDLFTDCTIPAAGVAFAVYIPGSEWGETKTTDVSGNVAFTELGSGRFVVAED
                     LRTIDTPLQRYTMFCFGEPVSPNAPEPRQVVLEDLGNGEYAVDLTSGEEIACTWFNIP
                     AALEGEPIATTVPQAVTALPTTGTGDAVDRSNRAAKALLVGGAVLAMLALGATCQRRM
                     A"
     gene            complement(5348..5908)
                     /locus_tag="VGR22_11330"
                     /pseudo
     CDS             complement(5348..5908)
                     /locus_tag="VGR22_11330"
                     /inference="COORDINATES: protein motif:HMM:NF025170.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PCYCGC motif-containing (lipo)protein"
     gene            complement(6028..7089)
                     /locus_tag="VGR22_11335"
     CDS             complement(6028..7089)
                     /locus_tag="VGR22_11335"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEV2129200.1"
                     /translation="MSTDRRFASSIDRRAILRGGVAAGIALPFGIPSLVNAQDHGHGT
                     PEDGYVGSDPSAPTTGNEEPRATEITPFQRYDPFLEPVEPGPKTIETVAADRALYVAK
                     DVPFAAWTFDGTVPGNKIRARVGDEIDFTFRIEESASTAHSLDFHSAEGNPHENYNTI
                     LPGEEFSWTFTPRHAGAYMYHCGTPPVLLHIGAGMYGAMIVDPEDGWPTEAQEIVLVQ
                     SDFYFEEEPNEDGIHQHDYGKMLGGGMMDYVVFNGHATQYVDEPIKVKAGEPLRIFVV
                     NAGPNVYSTFHVVGGIFDAGYFNANPENKIVGLQSMMVGPGDGIAVELTLKEPGVYPA
                     VNHAFGHAAHGAIALLEAE"
     gene            complement(7278..7661)
                     /locus_tag="VGR22_11340"
     CDS             complement(7278..7661)
                     /locus_tag="VGR22_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129201.1"
                     /translation="MTEPNQRVQYNAEMERTAYNRKVVSRAPASEVFALAREWFSERG
                     YRVLPSGRPNQIFIMGGPEGGLPRVNADILAVANVGKGKVTMLTFNSVGENLSARVAE
                     FVEMLRAQARAARREPTQEEREASN"
     gene            complement(7676..8368)
                     /locus_tag="VGR22_11345"
     CDS             complement(7676..8368)
                     /locus_tag="VGR22_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEV2129202.1"
                     /translation="MKLTYFGHSAFLFDDGQQHTVLLDPFMSSNPTCGTSPSDVNPDA
                     ILLTHAHNDHVGDTVSIATRSEAKVFATVELANFLSSQGVENVVGANHGGTVTFPGGT
                     AKFVPAWHSSSYTTEDGTVVASGVPAGFVIRFGGKTIYAAGDTALFMDMQLIGDEEID
                     IALLPIGDHFTMGPEDARKAIDYLRPGTVVPCHCNTFPAIEQDRDAFRNRVEGETATR
                     VVVMDPDTSRDF"
     gene            complement(8443..10035)
                     /locus_tag="VGR22_11350"
     CDS             complement(8443..10035)
                     /locus_tag="VGR22_11350"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEV2129203.1"
                     /translation="MKSTLYTGGTILTMNPKQPRAEALLVSDHLVRAVGTRADLEALG
                     GSGIEVINLDGATMLPGFNDNHTHPIKFGESLFQVDASPRAVTRLDDILEAFRKQLVQ
                     QPGDGWLHGRGYDDTRLDVQRHPTRDELDAITPARPILLVRTCGHLGVANTAALNAAG
                     ITDETPDPQGGQIDRDAHGRATGLLRETALKLVQRVVPEPTVAEMRTHIAAAGNVFLS
                     QGITSVADASVTRADQLAAYHSLAREGRMPFRVYTMHLIDETLDTLRSLGLQTGFGNE
                     WMRIGPAKVFQDGSGGGRTAGMFDPYVGQPNNYGITYYSQEELNERFEAAHAAGFQMA
                     AHAIGDRAITMILDAYEHALGAAARSDHRARIEHCGICTPEILRRLKALGVFAAPQAT
                     FVHYLGDSYLRNFTERQLTLAYPLHDFQQAGIRFSMSSDVPVTPCNSVINLHAATTRK
                     TQDGNDMAAGQAITMQTALRAYTTVGAFGTFEERLKGTLEPGKLADMVVLSADPTPLA
                     PEDLLKVSIQRTVLHGETVYQA"
     gene            complement(10032..>10559)
                     /locus_tag="VGR22_11355"
     CDS             complement(10032..>10559)
                     /locus_tag="VGR22_11355"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV2129204.1"
                     /translation="CSIVDLPTIQRVARLGVVPIPGTSFLHYTRPVYEQNLGRERFRY
                     AYAMKTYAEHGVVAAASSDAPVVPVDPLIGIQTMVTRKDRLGKDAWAEERVSVEEAIR
                     AYTVNAAYASFAEKRRGRLVPGMVGDITVFDQDLREMDPDQITTARVDLTVTDGEVVW
                     DRANAATREATEVLA"
BASE COUNT         1778 a         3248 c         3505 g         2028 t
ORIGIN      
        1 gccgcgagca gcagcaccgc caggacctcg ttgaccccac ctaggcggca ggcatcgagc
       61 tggcaaaagc gaattgcctc ggcctgaagg agctgcttga acatcacgcg attgtggcag
      121 tgctcgcccg tcgcaacgcc gattggcgcg accgctctgg cgatggtggc gtggccgaga
      181 atatcatcgg gactcgtcgg ttcctcgatc caccagggat cgaaccgcgc cagctgtcgc
      241 atctgcgcga cggcctcgcc gacgtcccac acctggttgg catccatcat cagtcggcgg
      301 tgagggccga tctcttctct aatgatcgcc gctcgccgca ggttgtcctc gatatccctc
      361 cccaccttca ctttgaagtg ggaccagccg ctggtgaccc cttcgcggca gagacggcgc
      421 agcttctcat cagagtagcc gagccagccc gctgacgtgg tgtaggcggg gtagccgtcc
      481 ctccgcagct gtgcctcccg gtcggctttc gttggcgcgt tgttgtggag gatcgccaag
      541 gcctcctcgg gcgtgatggc gtcggtgatg tagcggaacg ggacacaggc gacgagccgc
      601 tccggtggca ggtcagccag gagcttccaa agcggcttgc cctccgtctt cgcccacagg
      661 tcccagacag cgttgacgac ggcggcggtg gcgaggtgga tgaccccctt ctccgggccc
      721 acccagcgga gctggctgtc gcccgtgatc gtctgccaga aggcccccat gtcagcagta
      781 aacgactcga ggatcttgcc tcggacaagc ggtgcaagtg cctcgatggc cgcaacgcac
      841 agctcgttgc cgcggccgat ggtgaaggtg aggccgtgcc cctcgaggcc cagcggatgg
      901 tccgtctgca agatgacgta ggccgcggag tagtcgggat cggggttcat cgcgtcggaa
      961 ccatcgaatg tcttggaggt gggaaagcgg atatcgagcg cgaccaggtc ggtgatggtg
     1021 gttggcatca cgcctccttc ggctggggct gagttcaccg ccgccgagaa cgagcgctgc
     1081 gggctcggag cacgcacccg gagatgccag gccgcattcc atcctgaacg agtcaaaagc
     1141 agcatggtgg gccgaggtgg cgatgatccg agagccgaaa cagttcgctg gaactggcga
     1201 gtggagcagc agagtgtgcc cctgcgcgct ggtcttgttt ccgatcgatg cggactcgcg
     1261 cgctgatttt gaacgtggta ccccaggcag gaatcgaacc tgcgaccttt cgcttagaag
     1321 gcgactgctc tatccgctga gctactgggg tgcgaatggg ttgcgctggt actgctgccc
     1381 accgcaaaca catggggaat tgtaccaaag acacccttgg ctcccatgag cggagggagg
     1441 atcttctgcg tctggtattg tccgaacgtc gtatactggc ggtggtacct gtcgtctgcg
     1501 tacgccatct cgccggtggt cttgtcatgt caatcgaacc cgacgtcgcg ctgccggtcc
     1561 agcccaatcg tctcatcggt cgcgaggcga tatgcgcgca tgtcggcgac ctcgtgctcg
     1621 cggagcatca ccgcctagtg acgctgatcg gccctggtgg ggtgggcaag acccggctcg
     1681 cgatgtgcct cgccgaagac ctcgcggcga actttcccga cggcacctgg tttgtccccc
     1741 tggagcacct tcggacctcg gagctcgtaa tgcccgcgat tgcgcgtacc atgggcgttc
     1801 acgaagccct cggacaggtg ggcctcgatc tcctgatcac aacactccga gaccgccgcg
     1861 ctttgctcgt gcttgacaat ctggagcaag tcgcgggtgc tgcgtccgaa atcgctcagc
     1921 tgctggcggg ctgcaagtat ctgcgaattc ttggcacaag tcgagcgtca cttcggctga
     1981 gggcggagcg ggaggttccg gtcgagcccc ttgacacgcc ggctgcaggt agcgtggtgg
     2041 ccgcggcgga tattgccgag agtgcggcgg tcgagctctt cgtcgagcgg gctcgagcgg
     2101 ttcggccgac gttcaatttg tccaacttca ccgcgcccat catcgcggag atctgccgac
     2161 gcctcgatgg tctgccgttg gcgatcgagc tcgctgcctc gacaatcaac gtgctgtccc
     2221 cgcaggagct cctggatcgt ctgaacaacc ggctccaatt gctctccaac ggagcggtcg
     2281 atctcccggc gaggcagcga tcgctgcgca gcgcgatcgc ttggagccac gatttactcg
     2341 acgccaacga gagacgcctc ttcgagagcc tctccgtctt tgcgggcgag ttcggcatcg
     2401 aggcagcagc cgtgctgctg gacgagaacg acgagactgg cggggattcg gacgtcttgc
     2461 caacgctgtc gtcactggtc gacaagagcc tgctcaaccg catcgatgtg cctgacggtg
     2521 aagtgcggtt tcggatgttg tcgacgatca aggagttcgg cctcgagcgc ctggcggact
     2581 cgggtgacct cgtcgtcgcg cagcaccggc tggtagcatg ggcgttggat ctggcagagc
     2641 gggcagcccc ggagctgacg ggccccgagc aggcgcgatg gctctcgata ctcgagcggg
     2701 aacatgacaa tcttcgtgcg gcgctgatcg ttgcacgtga cgtcagcccc gaagaggggt
     2761 tgcgagttgc gagtgcgctc tggcggttct gggcaacccg cgggtacctt accgagggct
     2821 gttcctggct cgagcagtac gttgccgcga gtgacggtat cgaagatcgc tacctggcaa
     2881 gagccttggt tagcctgggg aatctcgcac tcgatctcgg tgcgtatgaa gatgcgatcg
     2941 tcagctacga tcacggtctt cgcctcttcg aaaaactggg cgaccagtgg ggcgtagcgg
     3001 atgccctgaa cggcacgggc ctggtggact ggtatcgagg tgaaaacctc aaggcccgtg
     3061 acgcacacga gcagagtctg gcgatccgtc gcgagctcgg aaaccgccaa ggcgaggcca
     3121 actcgctgtc caacctcgcc aatgcggtac gggatctcgg tgagattgag cttgcgggcg
     3181 aactgcatcg gcaggcgctg cagatccgga tgtcgcgggg agatcgaggt gcgaccggat
     3241 tctctctcgt gaacctcggc gacatctcgt ggcggctcgg cagggcggac gaggctcagt
     3301 cgttcttcca gcaaagtctt gacacctttc gggaggtcca cgacatgctg ggcgaggcct
     3361 atgcgcttct cggcattggc cttgcggccg cgatggcgaa cgatgccggc gaagcccggc
     3421 gttccatcgc cgactcgctg gcgatccggc tccgcttggg ggatcggcga ggggttattg
     3481 aatgcgtcga aggtattgcc gcgctcgccg ctcgaggacg ccaggaatcc aaggccgccc
     3541 gcctcttcgg tgccgctcag gcgcttcgcc gacagatcga cgctcccttg gcggaagcgg
     3601 accgtgagcg ccaagcccgg ctcgtggcga agctcaggcc cgcgctcgcc gattcggcat
     3661 tccgccaaga gtgggatgcc ggtgctgcgc tgaccatggc cgacgctgcg gaggaggcac
     3721 tgaaggccgc agtggcgtcg ggcgcgccga gggagtggcc tggcggactg agccagcgcg
     3781 aagtcgaagt gttgcgcctg gtggcggccg gcctcaccaa cggcgaggtg gccgatcggc
     3841 tattcctgag ccggcgcacc gtcgacgccc atatgcgacg catttacgac aagctgagtc
     3901 ttgcctcgcg acttgaggca attcgctatg ccactcagca cggcctcgcc tgaccgcaag
     3961 cacgtggcgt tccggaatag gtgagggcgt taggtagagc taccgatgcc ataaggtctt
     4021 cgtgatcctc agactggaga cgtgacgggt ggcgctgtgc gcctgttgag ttcaccacgt
     4081 aaaaggatca gcgagtcatg atgcagttcg ttcccggatc catgattgtc acgctggtgc
     4141 tgctggcgtt cgccggtttc ggcatgccca ctgctgcggc acagcccgct ccagccctca
     4201 tgagcatcaa cagctggctc tgccgctcgg actacgaccg gatctctgac tgcgagaaga
     4261 tcggtggggt gatcgtcagc atcaggtcag atggacaggt tgtgggtgag gtaaccactg
     4321 tcgcggattc cgcggttgaa atcgaggtgc tgcccggcca gagcgtgacc gcgtcgatcg
     4381 tcggtggtca gcccgaaggc acaaccctga agaacgcggg gctgtccttc acagccgccg
     4441 agcgccagaa ccctatcacg ctcgtcttct tggagcgttc tgggagcggg tcgatcgaca
     4501 gtgattctga tggcctctcg gacgaggagg aagctgcgta caggaccggc ccggacgtcg
     4561 cagattcgga tggtgatggg attcaggacg gcggcgaaat caacatcggc gccgacccgc
     4621 tggtggcgga taccgacggg gacgggttcc tcgacggcga ggagttcgag cgtgcgacgg
     4681 atcccctcga ttcgagcagc ttcccagtcg agaccgaacc caactcgatc gtcatcagcg
     4741 cgttcacctg cccggcgggc cacggtggaa aggacctatt caccgactgc accatcccgg
     4801 cggccggagt ggcctttgcg gtctacattc cgggaagcga gtggggcgag acgaagacca
     4861 ccgatgtcag cggcaacgtt gccttcacgg agcttggttc cggccggttc gtcgtcgccg
     4921 aagatctccg aacgatcgac acaccgctcc agcgctacac gatgttctgt ttcggcgaac
     4981 cggtctcgcc gaacgcgcca gagcctcggc aggtcgttct cgaggatctc ggcaacggcg
     5041 agtacgccgt cgacctgacg agcggggagg agattgcgtg cacgtggttc aacattccgg
     5101 ctgctttgga gggcgagccg atcgcgacga cagtgccgca ggcggtgacg gcgctgccga
     5161 ccactggcac cggtgatgct gtcgacagga gtaatcgggc tgcaaaggcg ctcttggttg
     5221 gcggcgccgt cttggcgatg ctggcacttg gcgcaacgtg tcaacggcgc atggcctgac
     5281 acgtcgagaa tgggcgcgga cacgagtgag cgcttggtca gtcatgatca agggctcact
     5341 cgtcggctca tccccacgtc atctcccagg gcggatatgg cgtcggtgtc gcatagtcca
     5401 tctgggcagc aaaggtgcgg tcgatggctg tgcgaatatc gtacggctgc attccctcgt
     5461 cgagcatggc gatcacatca cgcgtgacct gaagacatgt gcctcaggta ggtgccatgg
     5521 gatcgaacgt gactgagccg tcagcgtgaa ccgcctgaat gtaacagtct cggttgttca
     5581 tgtgcccgcc actggcgatg cagccgcaga agcagggcat gtagcgcatc aggccaccgt
     5641 tggtgacgtg gaaggcgtag aggcgctgaa tctccgggtc cgatgtgagc acgaaatccg
     5701 gccagacgac gcggtcaagg tcgccttcag gctcctcggc aagcgccaaa tgggcgaaat
     5761 gtgcttcgaa gttggcagcc cgcccctgct ttccagctcg gtcgtccgtg agccgccatg
     5821 tggtgactgc tcccgtcgcc ccggctgctc ccgtgatggc aaggaaccgc cggcgggtga
     5881 accgcgtttg gcgatgtcgc cgcgtcatcg ttcctcctgg cctgacctgt gtgtcgccgt
     5941 cagcgaccgc agcgtggggt cagcgcgtca gcggcatgtg gcagggttgc cgccctgcca
     6001 catgccgcgt gtcactgcac gggtgtccta ctcggcctca agcagtgcga tcgcgccgtg
     6061 ggcggcatgg ccgaaggcgt ggttgaccgc tgggtagacg cccggctcct tgagcgtgag
     6121 ttcgaccgcg atgccgtcgc cgggcccgac catcatcgac tgcagcccga cgatcttgtt
     6181 ctcggggttg gcgttgaagt agccggcgtc gaagatgcca ccgaccacgt ggaatgttga
     6241 gtacacgttc gggccagcat tcacgacgaa gatgcggagc ggctcgcccg ctttcacctt
     6301 gatcggctcg tccacgtact gcgtggcatg cccgttgaag accacgtagt ccatcatgcc
     6361 gccaccgagc atcttgccgt agtcgtgctg gtggatgcca tcctcgttcg gctcttcctc
     6421 gaagtagaag tcgctttgga ccaggacgat ttcttgcgcc tcggtgggcc agccgtcctc
     6481 cgggtcaacg atcatggcgc catacatgcc ggcgccgatg tgcagcaaca ccgggggcgt
     6541 gccgcagtgg tacatgtagg cacctgcgtg ccggggcgta aatgtccagg agaactcctc
     6601 gccgggcaga atcgtattgt agttctcgtg ggggttgccc tcggcgctgt ggaagtcgag
     6661 cgagtgggcg gtcgacgcgc tctcctcgat gcggaacgtg aaatcgatct cgtcgccgac
     6721 ccgagcacga atcttgttgc ccggaacagt gccgtcgaag gtccaggcgg cgaacggcac
     6781 gtcctttgcc acgtagagcg ctcgatcggc tgcaacagtc tcgatggtct tcgggcctgg
     6841 ctcgactggc tcgaggaacg ggtcgtagcg ctggaacggg gtgatctccg ttgcgcgtgg
     6901 ttcctcgttg ccggtcgtgg gcgcgctggg gtcggagcca acgtagccgt cttccggcgt
     6961 gccgtggccg tgatcctgag cgttcacgag actcgggatg ccgaatggaa gggcaatccc
     7021 ggcggccacg ccgccgcgga ggatagcgcg tcgatcgatg gaggaggcaa atcgtcggtc
     7081 ggtgctcata acgtctccct tgcttcacca cctgcgccat tcgctgtggt gcgacaccat
     7141 ggtcgattcc atgtggcgcg atgcgccgtc tggaaccgaa cgtgatgcaa gtttgtcgga
     7201 aacgccgtag cacgcccgtt acaattcgca accggacatg tcagtttcgt gacaattcgg
     7261 accgcttggc acagatccta gttgctggcc tccctctctt cctgtgtcgg ttcgcgccgc
     7321 gccgccctgg cctgggcccg gagcatctcg acgaactccg ccacgcgcgc ggacaggttc
     7381 tcgccgaccg agttgaacgt cagcatcgtg accttaccct tgcccacgtt cgcaaccgcg
     7441 agaatatcgg cgttgacccg gggcaggcct ccctccggcc cgcccatgat gaagatctgg
     7501 ttcggccgtc ccgacggcag cacgcgatag ccgcgctcgc tgaaccattc cctagccaag
     7561 gcgaagactt cgctcgcggg tgcacggctg accaccttcc ggttgtaggc ggttcgttcc
     7621 atctcggcgt tgtactgtac gcgctggttc ggctcggtca tcgcggcact cggactcaga
     7681 aatcgcggct ggtatccggg tccatgacca ccactcgcgt cgcggtctcg ccctcgactc
     7741 ggttgcggaa cgcatctcgg tcctgctcga tagccgggaa ggtgttgcag tggcagggga
     7801 caacggttcc ggggcgaagg taatcgatcg ccttgcgcgc gtcctccggt cccatcgtga
     7861 agtggtcgcc gatcggcagc agagcgatgt cgatctcttc gtcgcctatg agctgcatgt
     7921 ccatgaagag cgcggtatcg ccggcggcgt agatcgtctt gccaccgaag cggatcacga
     7981 atccggctgg cacacccgag gcgacgaccg ttccgtcctc cgtggtgtag gacgagctgt
     8041 gccacgccgg gacgaacttg gcggtgccgc cggggaaggt caccgtcccc ccatgattcg
     8101 cgccaacgac gttctcgacg ccttgtgagg agaggaagtt ggcgagttca acggtagcga
     8161 aaactttcgc ttccgagcgc gttgcgatgc tgacggtgtc gccaacgtgg tcgttgtggg
     8221 cgtgcgtgag cagaatcgca tccggattga cgtcgctcgg cgacgtaccg cacgtcggat
     8281 tgctgctcat gaacgggtca agcagcaccg tatgttgttg cccgtcatcg aagagaaacg
     8341 ccgagtggcc gaagtaggtg agcttcatct ccttctcctg tcccttgcat gcacggtcat
     8401 cttgcctgtg cacttccctg caacttcggt gccggggcac gctcaggcct gatacaccgt
     8461 ttcaccgtgc agcacggtcc gctggatgct gaccttgagc aggtcctcgg gcgcgagggg
     8521 cgtcgggtcg gcgctcagga cgaccatgtc ggcgagcttg cccggctcca gggtgccctt
     8581 cagtcgctct tcaaatgtgc caaacgcgcc cacagtcgtg taggcccgca gcgcagtctg
     8641 catcgtgatc gcctgaccgg cggccatgtc attgccatct tgggtcttgc gcgtggtggc
     8701 cgcgtgcagg ttaatcaccg agttgcaggg cgtcacggga acatccgaac tcatgctgaa
     8761 ccggatgccg gcctgctgga agtcgtggag cgggtaggcg agggtgagct ggcgttccgt
     8821 gaagtttcgg agatatgagt cgccgaggta gtggacgaat gtcgcttgcg gcgcagcgaa
     8881 gacgccgagc gccttgagcc gacggaggat ctccggcgtg cagattccgc agtgctcgat
     8941 ccgcgcccgg tggtcggaac gtgcggcggc tccgagcgca tgctcgtagg catcgaggat
     9001 catcgtgatc gcacgatcgc cgatcgcgtg ggccgccatc tggaagcccg cggcatgcgc
     9061 ggcctcgaat cgctcgttga gctcctcctg tgaatagtac gtaatcccgt agttgttcgg
     9121 ctggccaacg tatggatcga acatgccggc ggtgcggccg ccgccgctgc cgtcctggaa
     9181 caccttggcg ggaccgattc gcatccactc gttgccgaat ccggtctgga gcccgagcga
     9241 gcgcagggta tcgagcgtct cgtcgatcag gtgcatggtg tagacgcgaa acggcattct
     9301 cccttcccga gcgagcgagt ggtatgccgc aagctggtct gccctggtga cggaggcgtc
     9361 agcgacactg gtaatgccct gactgaggaa gacgttgcct gcggcggcaa tgtgtgttcg
     9421 catctcggca acggtcggtt ccggcacgac acgctggacg agcttgagcg ccgtttcgcg
     9481 gagcaggccc gtcgcgcgtc cgtgcgcgtc gcggtcgatc tggccaccct gcgggtccgg
     9541 cgtctcgtcg gttatgccag cggcgttgag ggcggcggtg ttcgccacac cgaggtgccc
     9601 acatgtgcgc acgagcagga tcggtcgcgc cggcgtgatg gcgtccagct catcgcgcgt
     9661 tgggtgccgc tgaacgtcga gccgggtgtc gtcgtagcca cgaccgtgca gccagccatc
     9721 acccggctgc tgcacgagct gcttccggaa cgcctcaagg atgtcatcga ggcgcgttac
     9781 ggcgcgaggc gaggcatcga cctggaagag gctctcgccg aacttgatgg ggtgggtgtg
     9841 gttatcgttg aatccgggca gcatggtagc gccatcaagg ttgatgacct cgattcccga
     9901 tccgccgagt gcttcgagat cggctcgcgt tcccaccgct cgaacaaggt ggtcgctgac
     9961 cagcaacgcc tcggcacggg gctgcttcgg attcatcgtc agaattgttc cgccggtgta
    10021 aagggtactt ttcatgccaa cacctccgtc gcctcgcgag tcgccgcgtt ggcacgatcc
    10081 cagacgacct cgccgtccgt cactgttagg tcgacgcgag cggtcgtgat ctggtccggg
    10141 tccatctcgc gaagatcctg gtcgaagacg gtgatatccc cgaccatccc gggtaccaac
    10201 cggccccgcc gcttctccgc aaacgaggcg taggcggcat tgaccgtgta ggcgcggatc
    10261 gcctcctcca ccgaaaccct ctcctcggcc caggcgtcct ttccgagcct gtccttgcgg
    10321 gtgaccatgg tctgaatgcc gatcagcgga tcgaccggca cgacaggtgc atcgctggac
    10381 gctgccgcaa ccacgccgtg ctccgcgtag gtcttcatcg cataggcgta tcggaagcgc
    10441 tccctgccga gattttgttc atagaccggt cgcgtgtagt gcaggaagct ggtcccggga
    10501 atcggcacga ccccaagcct cgcgacgcgc tggatcgtgg ggagatcgac gatcgagca
//
LOCUS       DASYWL010000322         6575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_204340, whole genome shotgun sequence.
ACCESSION   DASYWL010000322 DASYWL010000000
VERSION     DASYWL010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6575
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204340"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1687)
                     /locus_tag="VGR22_11360"
     CDS             complement(<1..1687)
                     /locus_tag="VGR22_11360"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEV2129205.1"
                     /translation="MARSFDNASLASIVAQGIDRPFPAIRDWSRDTIRGLRSTVLPEL
                     GSGAEPQPDVVAAVTPPATERRPHRVLVRRVLLATIVALLLLAVGLVVFRGVYAGRVY
                     PAVVVGDVPVGGLTIDDAQSRLTKRASDLERGMFTFSHEGQTWTPSLTEMGVTVNLDL
                     SLTQAEQLGRTGNAASRLGFVAEALSADHVVPLQTQIDRRMLAAWFDSVDRDIDQLPV
                     DARIAIESGEVSVLPGASGIVVDREAATELVLSALSSLEPITVKLPTMVAEPQINSDM
                     LTPIKGQVSEAIGTPITATLFGEKWEITGDTIAPYLMVDVSMKAGEPKANLTVDRDGL
                     ASELRTRFSDKVSRKPVNAVVAWDDGLVALEPSVTGAALRSDAFADEVAESFFNGHHD
                     VEIPAVIIEPEIDSSNLAKLGIKELLGTGHSNFAGGVEARDENIRVATRLMNGTLVPP
                     GGVFSFNGAIGEITADKGYQEALVVSGDQVGRDVGGGVCQVSTTVFRAALNAGMPIVE
                     WYPHTYRLPDYERDGWGPGFDASILQFGPNPDEWPDFRFENYSDSWLLVESYIT"
     gene            complement(2008..3144)
                     /locus_tag="VGR22_11365"
     CDS             complement(2008..3144)
                     /locus_tag="VGR22_11365"
                     /inference="COORDINATES: protein motif:HMM:NF017691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HEV2129206.1"
                     /translation="MKQTRLETMRTATSRMAGRMAALAVIAALWLAFATLASAQVDPV
                     SCGQFETQEDAQAYFDENELEDPAVLDPDADGIACEFAFGGNEGPPAVVDPVSCGHFA
                     TQPDAQAALEARPELAPVLDANGNGVACEEAFPGVVVDPTSCGHFETREDAQAALDEG
                     AVPNPENLDGDGDGIACEDAFDGEDDGSSEVPQMETIGLGIAKYNCPSAAAYPDDLAS
                     CELGAGVTFEVATADGVILGSCTTEITMVQDSEVAFCAVDNVGYDTPLVITEDVATGP
                     VGFVPLENPQTMTIERPSPDAADFAPRVTFVNVPAVDEEPSTGGTDTGDIDRDTDTVL
                     ALPQTGTGDASATDAGRVAFMVLVAAACGWIGLVMRVRSARQGA"
     gene            complement(3381..5765)
                     /locus_tag="VGR22_11370"
     CDS             complement(3381..5765)
                     /locus_tag="VGR22_11370"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2129207.1"
                     /translation="MPPTRLIGRAHEVVEVGALLSQPHVRLLTLTGPGGVGKSRLGLE
                     VATAQDDAFPDGIHMVSLATIHDPALVLPTVVRALGIRGDGSQSLMASLVEQLGGRRL
                     LLILDNFEQVVDASITVAELLAACPGLKVLATSRVALEIRAEHEYPVGPLEPTGDSRG
                     VRGSTTRVAPAVALFVERARAVCPGFELTAENTPAVAEICSRVDGLPLAIELAGARIR
                     VLPPDAILERLESRLDLLSRGPRDLPDRHQTMRDAIAWSYSLLGPDERELFRRCAVFV
                     GGFSLEAIEAVARDAGVSSVVNRLASLVRHNMLNSETVGGQARFRMLETIREFALERL
                     RAEGELDGTRCRHARWYLALAERAEPELVREQQRRWLDRLESEQGNMRAALDWLHSSG
                     DVEMELRLAAALWRYWATRGDLREGYDRLDRALARDGGAHADVRAKAHQHIGNLAVDL
                     GDYGAGRAHYEASLAIRRPQGDLTKIAEPLNGLGLLAFYRGDYTEARRCHEESLALRR
                     ASGDAHGLSNSLVNLGDVASAQGDYQRAELLHEEALAARRNLGNANGVASCLLNLGEI
                     AWGRGDRPLARSRFEASLALFREVGDRLGVGNALYSLGRVAHAEGKAGQAAALYTEAL
                     PLRRDVGDRRGIIECMEGIAAIASPDDVTLAVTLCAAAETLRVESDAPLRPVDRALQE
                     RALAVIRSRVGKMAFGKAWSAGSSMSMALALTEASRLAQRIGARRENLPAGLSAREVE
                     VLRLVATGMTTAQIANELFLSTHTVNAHLRRIYGKIGVTSRSAATRFFLDSGLD"
     gene            complement(6122..>6575)
                     /locus_tag="VGR22_11375"
     CDS             complement(6122..>6575)
                     /locus_tag="VGR22_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129208.1"
                     /translation="CTADEMRERILAVNRPSAPFHIVDGSAEGVDLIAEWKIVDAQWY
                     EIFTKAGLEKVFRISMKLDEAEKQVRKDQEYSVSWEAGVPTLSLEMSKFKGQKQTFQF
                     GTAYAFTEELRPGQVYNYRFSTGEMKKPIQEAVTGCGWTYKGIAFGRL"
BASE COUNT         1142 a         2301 c         2074 g         1058 t
ORIGIN      
        1 ccgtgatgta cgactcgacc agcagccagc tgtcggagta attctcgaac ctgaagtccg
       61 gccactcgtc aggattcggc ccgaattgca ggatcgaggc gtcgaacccc ggaccccagc
      121 cgtcgcgctc gtagtcgggc agccggtagg tgtgcggata ccactccacg atcggcatgc
      181 cggcattgag cgccgcgcgg aagacggtgg tcgacacctg gcagaccccg ccgccgacat
      241 cgcgcccgac ctggtcaccg gaaacgacca gtgcctcctg gtagcccttg tcggcggtga
      301 tctcgccgat tgcgccgttg aacgagaaga cgccgcccgg cggcaccagc gtgccgttca
      361 tcagccgggt cgcaacccgg atgttctcgt cgcgcgcctc gaccccgccg gcgaagttgg
      421 agtgcccggt gccgagcagc tccttgatgc cgagcttggc gaggttgctg gagtcgatct
      481 ccggctcgat gattaccgcc gggatctcga catcatggtg accgttgaag aagctctctg
      541 ccacctcatc ggcgaaggca tccgaccgca atgccgctcc ggtcaccgag ggttccagcg
      601 cgacaagccc gtcgtcccat gccaccaccg cgttgaccgg cttgcggctc accttgtcgg
      661 agaaacgcgt ccgcagctcg ctggccagcc cgtcgcggtc gaccgtgagg ttcgcctttg
      721 gctcacccgc cttcatcgag acatcgacca tgaggtaggg cgcgatggtg tcaccggtga
      781 tctcccactt ctcgccgaag agggtcgcgg tgatcggcgt accgatcgcc tcggacacct
      841 gccccttgat cggggtgagc atgtccgagt tgatctgcgg ctcggcgacc atcgtgggga
      901 gcttcaccgt gatcggctcg agcgaggaga gcgcgctcag caccagctcg gtcgccgcct
      961 cgcggtcaac cacgatcccg gacgctccag gcagcacgga cacctcgccg ctttcgatcg
     1021 cgatccgcgc atccaccggg agctggtcga tgtcccggtc gaccgagtca aaccacgcgg
     1081 cgagcatgcg ccggtcgatc tgggtctgga gggggacaac gtgatcggcg ctcaaggcct
     1141 ccgccacgaa cccgagccgg gacgccgcgt tgccggtgcg cccgagctgc tccgcctgcg
     1201 tgagggagag gtcgaggttc accgtcaccc ccatctcggt cagcgagggc gtccacgtct
     1261 gtccctcgtg gctgaaggtg aacatcccgc gctcgaggtc agacgcgcgc ttggtgagcc
     1321 gtgactgggc gtcgtcaatc gtgaggccgc caaccgggac atccccgacg acgaccgcgg
     1381 ggtagacccg cccggcatag acgccgcgga acaccacgag gccgacggcg agcagcaaca
     1441 gcgccacgat ggtcgccagc aggacacggc gaaccagcac ccggtggggg cgccgctcgg
     1501 ttgcgggtgg cgtcacagcg gcaaccacat caggctgcgg ctcggcaccg ctccctagct
     1561 ccggcaggac ggtcgatcgc agaccgcgaa tcgtatcccg cgaccagtca cggattgccg
     1621 ggaagggacg gtcgatcccc tgcgcgacaa tactggccag ggacgcgttg tcgaaagaac
     1681 gcgccatgct ctcttctctc accccacacc cctggcgacc tcgatcaggc ggtcgtcaac
     1741 gcgcgatttc gatgctgccc gctccccgcg cacgtcaacg cgcctcggaa gcgcgtagtg
     1801 tctccgggag cggggtcggc gcctcttccg ggcccctgat gaatcgggat gggccggttc
     1861 ggtcgtggtg tcattctcac ataaggcgca ccggtgtgcc actatgtcac tcgctgtcag
     1921 cggcaccacg ccaatcgtca acacctcccg tcccgaaaac cgggacggga ggttgtcgtg
     1981 atcgaccggg aagggcgcct tctcgggtca ggcaccttgg cgggcggatc gaacccgcat
     2041 cacgagccca atccacccgc acgccgccgc aaccagcacc atgaacgcga cgcgtccggc
     2101 atccgtcgcc gaagcgtcac ccgtgcccgt ctgtggcagc gccagcaccg tgtcggtatc
     2161 acgatcgatg tcaccggtgt cggtaccacc agttgacggc tcctcgtcga ccgcagggac
     2221 attgacgaac gtcacgcggg gcgcaaagtc cgccgcatcg ggcgacggac gctcgatcgt
     2281 catcgtctgc gggttttcga gcgggacgaa cccgacgggg ccagttgcaa catcctcggt
     2341 gatgaccagc ggcgtgtcgt accccacatt gtcgaccgca cagaaggcga cctcgctgtc
     2401 ctgaaccatg gtgatctcgg ttgtgcaact gccaaggatg actccatcgg cggtagctac
     2461 ctcgaaggtg acgccggcac ccagctcgca ggaggccagg tcatccgggt acgctgctgc
     2521 gctcgggcag ttgtacttcg cgatccccag gccgatcgtt tccatctgtg gaacctccga
     2581 gctaccatcg tcttcgccgt caaacgcgtc ctcgcaggcg atcccgtcac cgtcgccgtc
     2641 gaggttttcg gggttcggca ccgctccctc gtcaagggca gcctgggcat cctcccgcgt
     2701 ctcgaaatgt ccgcagctcg tcgggtccac gacgacgccc gggaaggcct cctcgcacgc
     2761 gaccccgttg ccgttcgcat ccaggaccgg ggcaagctcg ggacgagcct cgagggcggc
     2821 ctgggcgtct ggttgtgtgg cgaagtgacc gcagctgacc ggatcgacca ccgcgggcgg
     2881 gccctcgttc ccgccaaagg cgaactcgca agcgatgcca tctgcatcag gatcgagcac
     2941 ggcaggatcc tcgagctcgt tctcgtcgaa gtaggcctgc gcatcttcct gggtctcgaa
     3001 ctgcccacaa ctcaccgggt cgacctgcgc ggaggccagt gtcgcgaacg ccaaccagag
     3061 cgcggcgatg accgcgagcg cggccatacg ccctgccatg cgggaggtgg cggtacgcat
     3121 cgtttccaac ctggtttgct tcatcgttct ctccttgcgc gcgtttacgg cgacgtctcg
     3181 aggtgtgact ggaggccaca cgccgatcct agcggcgtcc gccgcgcgtt ccatcgcaag
     3241 aactgactaa tcgccggtgg cacgctccca cgccggcaag cagagcccgc gctcacgcgc
     3301 ggacctcccg ctctgtatcc aggcacggag agtggggagc acgccggccc agccgttcgc
     3361 cttgccggcg tgcacccgta tcagtcgaga ccggagtcga ggaagaagcg ggtggcagca
     3421 ctgcgagagg tcacaccgat cttgccgtag attcgacgaa ggtgggcatt cacggtgtgc
     3481 gtgctcagga agagctcgtt ggcgatctgc gcagtggtca tgccggttgc caccaatcgc
     3541 agtacctcga cctcacgcgc gctcagaccg gcaggaaggt tttcccggcg tgcaccgatg
     3601 cgttgagcca gccgggacgc ctcggtcagc gccaacgcca tggacatgct cgacccggcc
     3661 gaccacgcct tgccgaaggc catcttgccg acacgcgacc ggatcaccgc caatgcccgc
     3721 tcctggagag cgcgatcgac gggtcgcagg ggcgcatcgg actcgacgcg cagggtttcc
     3781 gcggccgcgc agagggtcac cgcaagcgtc acgtcgtccg ggctcgcaat cgcggcgatc
     3841 ccctccatgc actcgatgat gccccgcctg tcgcccacat cccgccggag cggcaacgcc
     3901 tcggtataga gggccgctgc ttgcccagct ttcccctcgg cgtgggcgac gcgccccagg
     3961 ctgtacagcg cgttcccgac acccagacga tcgccaacct cccggaagag cgcgagactg
     4021 gcttcgaacc tgctgcgagc gagtggccgg tcaccacgtc cccacgcaat ctctcccaga
     4081 ttcaacaggc aggaggcaac tccattggca ttgcccagat tgcgacgagc cgccagcgcc
     4141 tcctcatgca gaagctcggc ccgctgatag tcgccctgcg cggaagctac atcaccgagg
     4201 ttcaccaggg aattgctcag gccgtgggca tcgcccgacg cacgccgcag cgccaggctc
     4261 tcctcatggc atcggcgcgc ttcggtgtag tccccacggt agaaggcgag cagcccgagc
     4321 ccgttgagcg gctccgcgat cttggtcaga tcgccctgcg gacgcctgat ggcgaggctc
     4381 gcctcatagt gggctcggcc ggctccgtag tcaccgaggt cgaccgccag gttgccgata
     4441 tgctggtgcg cctttgcgcg gacgtccgca tgcgcaccgc cgtccctggc cagcgcgcga
     4501 tccagccggt cgtacccctc acgcaggtca ccccgagttg cccagtagcg ccagagggca
     4561 gcggccaggc gcagctccat ctcaacatcg ccggacgagt gcagccagtc gagcgcagcg
     4621 cgcatgttgc cctgctcaga ttcaaggcga tccagccagc gccgttgctg ctccctgacc
     4681 agctccggct ccgcccgctc ggcaagcgcc agataccagc gcgcatgccg gcaccgcgtc
     4741 ccgtcgagct cgccctcggc ccgcagacgc tcgagcgcga actcgcggat ggtctcgagc
     4801 atccggaagc gggcttgtcc gcccacagtc tcgctgttga gcatgttgtg acgtacgagc
     4861 gatgcaagac ggttgaccac ggaggacacg cccgcgtccc ttgcgacagc ctcgatcgct
     4921 tcgagcgaga acccaccgac gaagaccgcg caccgccgga acagctcccg ctcgtccggt
     4981 ccgagaaggc tataactcca ggcgatggcg tcgcgcatgg tctggtggcg gtccggcaaa
     5041 tcgcgaggcc cccgagagag cagatccagg cgcgattcga gccgttccag tatcgcatca
     5101 ggtgggagga ccctgatacg ggccccggcc agctcgatcg ccagcggcaa gccgtccaca
     5161 cgcgagcaaa tctcggcgac ggccggcgtg ttctcggcgg taagctcgaa gccgggacag
     5221 accgcacggg cccgctcgac gaagagcgct acggcaggag cgacgcgggt cgtcgaccca
     5281 cggacacctc gcgagtctcc cgtcggctcc agcggaccga ccggatactc gtgctcggca
     5341 cgaatctcca gcgccacgcg acttgtcgcc aagaccttca atccggggca ggcggccagc
     5401 agctccgcaa cggtgatcga ggcatcgacc acctgctcga agttgtccag aatcaggagg
     5461 agccgccgtc cgccgagctg ctccaccagc gacgccatga gggactggct gccatctccg
     5521 cgaatgccga gcgctcgaac caccgtgggc agcaccaagg cagggtcgtg gatcgtggcg
     5581 aggctcacca tgtgaatgcc gtcgggaaag gcgtcatcct gcgcggtcgc cacctccagg
     5641 cccaagcgcg acttgccaac cccgcccgga ccggtcagtg tgagcagacg cacatggggc
     5701 tggctcagca gtgccccaac ctcgaccacc tcgtgagccc ggccgatcaa ccgtgtcgga
     5761 ggcacgacta tcccagcgcc cggcggagca ggatgacgcg gaggtgcaag cagacgaacc
     5821 gcttgatcgt gcgaagaggc atggctggtc aagggtgggc tcctcggggc ggcttcctgg
     5881 acggtaacag ccgggatgct gcccggaggc agcaccttgg ccaagcgaac gccgatgcca
     5941 atgaaacgcg gccagcgaat cgcacgtttc gggagcgact tctctggccg caccagggtg
     6001 attgccaaga gcatgactca tacgagccat caccgtcaca caaacaccaa cgggcacctc
     6061 tcaagcgggt gcccgtcgtc ttgtcacgga cgtgacgtgt gttgcccggg tgggtcggac
     6121 tctacagtcg gccgaaggcg atgcccttgt aggtccagcc gcaaccggta accgcctcct
     6181 ggatcggctt cttcatctcg ccggttgaga accgataatt gtagacctgc cccggccgga
     6241 gctcctcggt gaaggcatac gccgtcccga actggaacgt ttgcttctgg cccttgaact
     6301 tgctcatctc gagcgaaagg gtcggcacgc cggcctccca cgagaccgaa tactcctgat
     6361 cctttcgcac ctgcttctcg gcctcgtcga gcttcatgga gatcctgaac accttttcga
     6421 gcccggcctt ggtgaagatc tcgtaccact gcgcgtcgac gatcttccac tcggcgatca
     6481 ggtcgacccc ctcggcactg ccgtcaacga tgtgaaaggg cgcgctcggg cggttcaccg
     6541 ccaggattcg ctcgcgcatc tcgtctgccg tacac
//
LOCUS       DASYWL010000323        10635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_167170, whole genome shotgun sequence.
ACCESSION   DASYWL010000323 DASYWL010000000
VERSION     DASYWL010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10635
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167170"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(648..1460)
                     /locus_tag="VGR22_11380"
     CDS             complement(648..1460)
                     /locus_tag="VGR22_11380"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEV2129209.1"
                     /translation="MLRNPFRRVAVALILGIVLLFPPLASAQMADDVTPGITATGYGQ
                     ASAPADSATIQLSIASESFGPPQPMPSGGTPGAAERNTVAPIVKALVDAGIPEDTINV
                     LTGPYVTAVPSMYGPTMAMIRFEVPNPTANTISSAVDAAIIGAADARLIIGALSVQLE
                     SADCAALEHDARAAAIADAREQAEVLAELTGLTTGAVVAVRDVPTGPESGYMFAGPTF
                     LSGCSPFESDALNLDVYGGASYDPTQEPMVTAYAQVEMTFSTAAGPVATPAG"
     gene            complement(1650..2495)
                     /locus_tag="VGR22_11385"
     CDS             complement(1650..2495)
                     /locus_tag="VGR22_11385"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2129210.1"
                     /translation="MPSMLDHIVIVDRDLDTMVQQAESLGFTVVPGGQHAGGTTHNAL
                     IAFRDGSYIELIAFIDPEQRSTHRWWTRLWKGGGLVDFALFCSDLEADVAEIRDRGLE
                     IDEPVENGRLRPDGERLEWRQSFPQEIAGESGLPFLIEDITPRSLRVPSEEDQVTHRN
                     GVTGIAGLTLLVDDLGNAVTALEAITGNAAETIDPPFLGITAAQRITIGHELGQWVVV
                     ATPDMDQRESVGEEGLPAKYLEKYGLGPFSAVLTTGAAPADLAPDAGTEIDLQLLAAS
                     RLRIA"
     gene            2667..3554
                     /locus_tag="VGR22_11390"
     CDS             2667..3554
                     /locus_tag="VGR22_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129211.1"
                     /translation="MSITQKLSRRRFAGLSAAAIGATALGAPVASSTVAQSIDPFTYI
                     TTMTETETLGQVAGQPVEEVGPVEVDGALWTAMVQVPIKRGQDLHYTCEFDTAWSIMM
                     AYGLDVPLEEQLAAVGIDDRFEPYWEETARGVMIYGGDIGEYFCGDLDTNLVAKAKGS
                     AMAKAFEAMGLSVTPVYDRMAIEQSLNMGQPVFFKSTVDFLPWTPALWNTPEGKQYRV
                     VFTNDHALTVMAYNDREVVIRDPLGPTSTNYNRPYQYRVSWERFLEVFAAQENDGLAI
                     GPSAPAAPPVGDGTGGADL"
     gene            complement(3676..3948)
                     /locus_tag="VGR22_11395"
     CDS             complement(3676..3948)
                     /locus_tag="VGR22_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129212.1"
                     /translation="MRRLLISVSLAYLLVAFGFILLTMLNASNEGDFVAMAEADAGMI
                     VALSVFGVLFVIVLAIRILLDGKRRHDLERQLSEPRRKSQTAPRQE"
     gene            complement(3962..5473)
                     /locus_tag="VGR22_11400"
     CDS             complement(3962..5473)
                     /locus_tag="VGR22_11400"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEV2129213.1"
                     /translation="MDALGSLIDGFSVALTPENLLWALVGVFLGTFVGVLPGIGPALT
                     VALLLPVTYALDPVPAFIMFAGIYYGGMYGGSTTSILLNTPGESASIITALEGNMMAK
                     KGRGATALATAAIGSFVAGTIATLGITFAAPFMADIAIDYFQSPEYLALIILAFTTVA
                     TLLGRSMVRGLVSLFIGLALGLVGIDALTGQSRFTFGNQQLLGGIDVVTVAVGLFAVG
                     ETFWVASHMRKDTGEVTTVTDSKWMTKDDWKRSWKPWIRGALLGFPIGSMPAGGAEIP
                     TFLSYSVEKRLSKHPEEFGEGAIEGVAGPEAANNASAAGVLVPLLTLGLPTSATAAIL
                     LAAFQRYNLNPGPQLFDQNPELVWGLIASLYIGNLMLLVLNLPLVGMWVKLLSIPRPL
                     LYSGILVFAGLGTWAQSQSVTGLVVLYVIGALGYLMRRFDIPVGPAVVAVILGPMAEI
                     HFRRALQISQGDWSVFYNRPVSGTILVIAALAFIAPAVWRAVQARRQHPAATT"
     gene            complement(5553..6140)
                     /locus_tag="VGR22_11405"
     CDS             complement(5553..6140)
                     /locus_tag="VGR22_11405"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEV2129214.1"
                     /translation="MVEQAPVTDHSPQSTAPPDEPQHGQEGPQEPLSFWQRWGELFMA
                     VGVLVLGIVVLVESQDIRVRQGVVVSPRIIPQIVGAGLVLVAIWYVIDVIRGPHPGGA
                     GGEDAEDVDPEAKTDWSVIGIIAIGLVAYAALMEGVGFIVASAVLFVISAFAMGSRRI
                     VRDVAIGTVLAVGIFLLFDRWLGVRLPDGWVASQL"
     gene            complement(6222..7280)
                     /locus_tag="VGR22_11410"
     CDS             complement(6222..7280)
                     /locus_tag="VGR22_11410"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEV2129215.1"
                     /translation="MSTHTGRTGPLRIMAAVAVVLSFLLPGAVSVAQEVDCSEWASET
                     LEIMAPASPGGGWDTTAREIQRVLQEEGIVENVEVFNVPGAGGTIGLAELVNEHAGNE
                     HMLMMMGLVMVGGIVLNESPVDLTQVTPIARLTTEYEVIVVPADSEYQTIDELLTAFQ
                     EDPSSISWAGGSAGGTDHLLVGLLAEELGVDPAAINYVPFSGGGEALSAILGGETTAG
                     VSGLGEWQAQIESGELRALAVSGRGAAAPGSATPTAGEATPAAFGANIPTLQEAGYDV
                     ELANWRGFVAPPDISEEAETCMIQALEQAVQSEAWQTTLETYGWNDFFLAGDAFGAYL
                     QEEHERVTALLSDLGLTE"
     gene            complement(7532..8533)
                     /locus_tag="VGR22_11415"
     CDS             complement(7532..8533)
                     /locus_tag="VGR22_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129216.1"
                     /translation="MGLGPFTLISLIYVVIGIRSVSRMIANWGPVWDRRFSVQDRQLV
                     DEAAFFVLVPISVALHELGHAVAVWGYGGEVVDFGYYVFAGYVSFIPFGFSEVELFVV
                     AAAGTIVNFILSLGALAIVFFNPRPFRAAINELLVQFAIISAANAFIVYPLLDLVSGM
                     NGDWRQMYGSGVPLLTALIVAMQFGMVALGYLLYTNDAIKARLAMLTDVPAGMERGFL
                     GGVRPGKIDVRALTPRERVLREATERVQSGWPVRIETGIQRFDAGTAMTLAWQRNGQG
                     YAVAARAFTAGPADIVALNGRTAGAAPRLITRWPQVPDTDELTIALRVAMETVEQGS"
     gene            complement(8576..9913)
                     /locus_tag="VGR22_11420"
     CDS             complement(8576..9913)
                     /locus_tag="VGR22_11420"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2129217.1"
                     /translation="MPPDPPDTASPAAGSPRQHPGRRSRFSGLRKGFVALTYRNYRLF
                     WLGQLVSVTGTWMQSLALSWLVYEILGATPFQLGLVNVLQFLPVLLLGIPAGVIADRF
                     PKRRILLTTQSAMMVLSAILAVLVITDRVLLWQVYVLATGLGMARALDMPTRQAFVSE
                     MVEKDDLMNAIALNAALFNTGRIVGPALAGALLAVVGPGPLFAINAASFIAVLAGLLL
                     MRTRPITGTSEESPLQRLRDGLAYVRRDPLISRTVLMVGTIGIFGMNFNIWIPVLASD
                     EFAAGSSAYGTLFAAMGAGSLIGALSLAFFGQGPSRRRMIAAALLMGSAELVLALAAA
                     NGTAVMLGAAALALVGFASTNTMSTANTLVQTAASDELRGRVMAVYMTVFAGSIPIGA
                     LIAGWVTGQFGVATSLVMGGTLILAAAAFQILSTRHMRAAEPASHAPEAKPAD"
     gene            complement(10024..10479)
                     /locus_tag="VGR22_11425"
     CDS             complement(10024..10479)
                     /locus_tag="VGR22_11425"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV2129218.1"
                     /translation="MTSRILIPLDGSRTSEAALDEAIRQATAFDLPVHLVRVVDTHVL
                     EQVGGSAAAFNYTVLGEMFEQESAEAGSYLDEITKRLEGEGLTATREVLIGPIARSIL
                     DEVESGDMIVMGSHGRSGIRRWVLGSIAEDVLRDAYVPVLLVKHPHPQS"
BASE COUNT         1896 a         3369 c         3609 g         1761 t
ORIGIN      
        1 atcgacttcc tggtgccgtg gaacctgatc gtcatcacgc tgacgacggc aatcgtcgcg
       61 gcgctgctga tggcgtggat tcccgcccgg caggcgtcgc gcatcctccc cgccgaggcg
      121 ctccggtatg agtaggactc cagcgtaccg cgacacgatg ggtgcaacgt cagagccggg
      181 aaaatcccgg ctctgacact tggcgattgc cgctcggtag tgcgcgtcct gctgaacgtt
      241 gcgtatgcgg aactgaaccc gtcgcacaaa tggacatccc ttcgccgcgg ctcaggctgt
      301 cagagggtgt gttggcaaac cgatgggcgc ggagggtacc gcgcccagga cagcgagcac
      361 atcgctgccg ccaggttggc cgtcgatttg gtcgtcacgt ccgcgaggag gagtaaggaa
      421 tggaccaagg ccgagcggac gcagccacgt gttcgcatag tgctcgacgg ccagcgagat
      481 cctgtccttc acgaggtgtc cagacgattc gcctcgatgc ttgcgcgcct gccacagccg
      541 gataccggtt ttggcgtcga ggcgcccgaa ccggtcactg gcggcgagta ggtgcaaaga
      601 aacggccccg gatccatctg ggatccgggg ccaagctgtg tttctggcta tcctgccggg
      661 gttgccacgg gtcctgccgc agtcgagaag gtcatctcga cctgggcgta cgccgtgacc
      721 atcggctcct gagtcggatc gtagctcgcc ccgccgtaga catccaggtt cagtgcatca
      781 gactcgaacg gcgagcagcc gctcaggaac gtcggaccgg cgaacatgta gcccgattct
      841 ggtccggtgg gcacgtcacg cacggcgacc acagcaccgg tcgtgagccc ggtcagttcg
      901 gccaggacct cagcctgctc tcgtgcgtcg gcgatcgctg cggcgcgggc atcatgctcc
      961 aaggcggcac aatccgcgct ctcgagttgc acgcttagtg cgccgatgat cagccgggca
     1021 tcggcggcgc cgattatcgc ggcatcgacg gcgctgctga tcgtgttagc ggtcgggttg
     1081 ggcacttcga agcggatcat cgccatcgtg gggccgtaca tcgagggcac agcggtgaca
     1141 tacggtccgg tgagcacatt gatcgtgtct tcggggattc cggcgtcaac gagcgccttg
     1201 acgattgggg cgacagtgtt tcgctccgcg gcgcccgggg tgccgcccga aggcatgggc
     1261 tggggcgggc cgaagctttc tgaggcgatt gagagctgga tcgtggcgga gtccgccggg
     1321 gcgctggcct gcccgtaccc ggttgccgtt atgccgggtg tgacgtcgtc tgccatctgt
     1381 gccgaagcaa gtgggggaaa gagcagaacg atcccgagga tgagcgcgac ggctacccgc
     1441 cgaaaaggat tcctgagcat tatgcatgcc tcccggcttg tcccgattgg tcgtgttcca
     1501 atcgtactcc cttgatgctc gatccagaag atggcggctg aaacggtccg aatggcggcg
     1561 agatggaggc tcaggatgcg ccggagccta ggtctcacta cccgccggcg gtcacctgga
     1621 ggtcgaggcg caactggatc gaacaccgtt tacgcgatcc gcagacggga ggcggcgagc
     1681 agctggaggt cgatctccgt gccggcgtcc ggggcgaggt cggctggcgc cgcgccggtt
     1741 gtcagcaccg ccgagaaggg cccgagcccg tacttctcca ggtacttcgc cggcagaccc
     1801 tcctcgccga ctgactcgcg ctggtccatg tcgggcgtgg cgacgacgac ccactgaccg
     1861 agctcgtggc cgatcgtgat acgctgtgcc gccgttatgc cgaggaacgg cgggtcgatc
     1921 gtctccgccg cgtttccggt gatcgcttcc agtgccgtga cggcgttgcc gaggtcgtcc
     1981 accagcaggg tgagacccgc gatgccggtc acgccgttgc gatgggtgac ctgatcttcc
     2041 tcactgggca cgcggagcga tcggggggtg atgtcctcga tgaggaaggg cagaccgctc
     2101 tccccggcga tctcctgcgg gaacgactgc cgccactcca ggcgctcgcc gtccggtcgc
     2161 agccggccgt tctcgaccgg ctcgtcgatc tcaagcccgc ggtcgcgaat ctcggcgacg
     2221 tcggcttcca gatcgctgca gaagagcgcg aagtcgacca gcccgccgcc cttccaaagg
     2281 cgtgtccacc agcggtgcgt ggagcgctgt tcgggatcga tgaaggcgat cagctcgatg
     2341 tagctgccgt cccggaaggc gatcagggcg ttgtgcgtcg tgccgccggc gtgctggccg
     2401 cccggcacca cggtgaaacc gagcgattcg gcctgctgga ccatggtgtc gaggtcgcgg
     2461 tcgacgatta cgatgtggtc gagcatcgag ggcatggtgg atcctttcac gttgctcacg
     2521 cagcctggtt ttggcagtgt ggcacgggca cgtgagcgcg gcaatccatt ggcggcgctt
     2581 tatgtacaat gtcgacgtgg cacgtccggt atcgttctcg caagccagcc atccctgaaa
     2641 ggtccacgat gacccggagc actcccttgt cgatcacgca gaagctctcg cggcggcgtt
     2701 tcgccggact ctccgcggcc gccatcggcg ccacggcgct cggcgcgcct gtcgcctcat
     2761 cgaccgtagc gcagtcgatc gacccgttca cctatatcac gaccatgacc gagaccgaga
     2821 cactggggca ggtcgccggt cagccggtcg aggaggtcgg gccggtcgag gtcgatgggg
     2881 cgctctggac agcaatggtg caggtgccga tcaagcgcgg ccaggatctc cactatacct
     2941 gtgagttcga cacagcgtgg agcatcatga tggcctacgg actcgatgtg ccgctggagg
     3001 agcagctggc tgcggtcggg atcgacgacc ggttcgagcc ctattgggag gaaaccgccc
     3061 gaggcgtcat gatctacggc ggcgacattg gcgagtactt ctgcggcgac ctcgacacca
     3121 acctcgtggc caaggcgaag ggcagtgcca tggcgaaggc gttcgaggcc atggggttgt
     3181 cggtgacgcc ggtctatgac aggatggcga tcgagcagtc gctcaacatg gggcagccgg
     3241 tctttttcaa gtcgacggtg gatttcctgc cgtggacgcc ggcactgtgg aacacgccgg
     3301 agggcaagca gtatcgggtg gtctttacca acgaccacgc gctgacggtg atggcctaca
     3361 acgatcggga ggtcgtcatc cgtgatccgc tgggtccgac ctcgaccaac tacaaccggc
     3421 cttaccagta ccgggtctca tgggaacgat tcctcgaggt cttcgccgcg caggagaacg
     3481 atgggctggc gatcggcccg tcagctccgg cagcgcctcc cgtcggcgac ggcacgggcg
     3541 gcgctgatct atagggtcac atcctttgct gcctacaaca cgggctcccg tgtccgcccc
     3601 aaggggtcgg ttccccgccg atcacacaca tattctgtat aacgccttgc gccaatggtt
     3661 cacgcgggag ccgtatcact cctgacgggg cgccgtctgg ctcttgcggc gcggctcgct
     3721 gagctgccgc tcgagatcgt gccggcgctt gccgtccagc aggatgcgga tggcaagcac
     3781 gatcacaaac agcacgccga agacggagag ggccacgatc atgcccgcgt cggcttcggc
     3841 catcgcgacg aagtccccct cgttggatgc gttcagcatg gtcagcagga tgaacccgaa
     3901 agcgacgagc aggtaggcga gtgataccga gatcaacagg cggcgcacgg cggtctccgg
     3961 atcaggtagt ggcggctggg tgctggcgtc tcgcctgcac agctcgccag acggccggcg
     4021 caatgaaggc aagggcggcg atcaccagga tcgttccgga aaccggccgg ttgtagaaca
     4081 cgctccagtc accctggctg atctgcaatg cccgccggaa gtgaatctcc gccatggggc
     4141 cgagaatcac cgccaccacc gcggggccga ccgggatgtc gaagcgccgc atgagataac
     4201 ccagcgcgcc gataacgtac agtaccacca gcccggtgac ggattgactc tgggcccagg
     4261 tgccaagccc ggcgaagacg aggatgccgc tgtagagcag tgggcgcgga atcgacagca
     4321 gcttcaccca catgccgacc agcggcaggt tgagcaccag cagcatcagg ttgccgatgt
     4381 agaggctcgc gatcaggccc cagacgagct ctgggttctg gtcgaagagt tgcgggcccg
     4441 gattgaggtt gtagcgctgg aaggcggcca gcagaatcgc cgcggtcgcc gaggtcggta
     4501 gcccgagtgt cagcaggggc accagcacgc cggcggcgga ggcattgttg gccgcttcgg
     4561 gcccggccac gccctcgatg gccccctcgc cgaactcttc gggatgcttg ctgagtcgct
     4621 tttcgaccga gtacgagagg aaggtcggga tctccgctcc gccagccggc atcgagccga
     4681 tcgggaagcc gaggagggcg ccgcggatcc atggcttcca ggagcgcttc cagtcgtcct
     4741 tcgtcatcca cttggagtcg gtgacggtcg tcacctcgcc cgtatccttt cgcatgtggg
     4801 atgcgaccca gaaggtttcg ccgactgcga agaggccgac cgcgacggtc accacatcga
     4861 tcccgccaag cagctgctgg ttaccgaagg tgaagcggga ttggccggtc agcgcatcga
     4921 tgccgacgag gccgagcgcg agcccgatga acaggctgac gaggccgcgc accatcgatc
     4981 tgcccaggag ggtcgcgacg gtggtgaagg ccaggatgat cagcgccagg tattccggtg
     5041 actggaagta gtcgatggcg atgtcggcca tgaacggtgc cgcgaaggtg atcccgagcg
     5101 tcgcgatcgt cccggcaacg aaggagccga tcgccgccgt cgccaaagca gtggcgccgc
     5161 ggcccttctt ggccatcatg ttgccttcga gagcggtgat gatcgaagcg ctctcgccag
     5221 gggtgttgag cagaatggac gtcgtcgagc cgccgtacat gccgccgtag tagatgccgg
     5281 cgaacataat gaaggcgggg actggatcga gcgcataggt gaccggcagc agcagcgcga
     5341 cggttagcgc cgggccgata cccgggagca cgccgacgaa tgtgccgaga aagacgccga
     5401 ccagcgccca gaggagattc tccggtgtga gggcgacgct gaacccgtcg atgaggctgc
     5461 cgagtgcgtc cattcgcggg cctggctccc tgacttagcc tccgcggcca cgcgcacacg
     5521 gaggcgaagg atctatcgtt ggcatgatcg catcacagct ggcttgcgac ccagccgtct
     5581 ggcaggcgca cgcccagcca gcggtcgaaa agcaggaaga tgccgaccgc gagcacggtc
     5641 ccgattgcca cgtctcgaac gatccggcga ctgcccatcg caaacgcgga gatgacgaag
     5701 agcacggcac tggcgacgat gaagccgacc ccttccatca gtgcggcgta tgcgacgagc
     5761 ccgatcgcga tgataccgat cacggaccag tccgtcttgg cctcggggtc gacatcctcg
     5821 gcgtcctcgc caccggcacc gccaggatgc gggccgcgga ttacatcgat gacataccag
     5881 atggcgacca gcaccagccc tgccccgacg atctgcggga tgatgcgggg gctaacgaca
     5941 acgccctgcc tcacacgaat gtcttgagac tcgaccaaca cgacaattcc gagcaccagc
     6001 acgccgacgg ccatgaacag ctcgccccag cgctgccaga acgagagcgg ttcctgcggc
     6061 ccttcttggc catgctgtgg ctcatccggt ggggcggtgg attgaggcga gtggtccgtg
     6121 acaggtgcct gttcgaccat cgcgcgtctc cagtatatgg acaagccgcg ttttcacgcg
     6181 gcttgtccgt tgcatggcac gattgggcgc gccggtgaac actactcggt caggccgagg
     6241 tccgagagca gggcggtgac ccgctcgtgc tcttcctgaa ggtaggcgcc gaacgcgtcg
     6301 cccgcaagga agaagtcgtt ccagccgtag gtctccagcg ttgtctgcca agcctcggac
     6361 tgcaccgcct gctcaagcgc ctggatcatg cacgtttcgg cttcctcgga gatgtccggt
     6421 ggggcgacga agccacgcca gttggcaagc tcgacatcgt agccggcctc ctggagcgtc
     6481 gggatgttcg cgccgaatgc agcgggcgtc gcctcaccag cggtcggggt agccgagccg
     6541 ggtgctgccg cgccacgacc ggagaccgcg agcgcgcgca gctcaccgct ctcgatctgg
     6601 gcctgccact caccgagacc ggagacgccg gcggtcgtct cgcctccgag gatggcagag
     6661 agcgcctcgc caccaccgga gaacgggacg tagttgatcg cagccgggtc gacgccgagc
     6721 tcctcggcca aaaggccgac cagcaggtga tccgtcccac ccgcggagcc gccggcccag
     6781 ctgatgctgg acggatcctc ctggaaggcg gtgagcagct cgtcgatggt ctggtattcg
     6841 gagtcggccg gcaccacgat cacctcgtac tcggtggtga gtcgggcgat cggggtgacc
     6901 tgggtgagat cgaccggcga ctcgttgagc acgatgccac cgaccatcac gaggcccatc
     6961 atcatcagca tgtgctcgtt gccggcatgc tcgttcacca gttcggcgag accgatggtg
     7021 ccgcccgcgc cgggtacgtt gaagacctcg acgttctcga cgataccctc ttcctgcaac
     7081 acccgctgaa tctcgcgtgc cgtggtatcc cagccgccac cgggggaggc tggtgccatg
     7141 atctcgagcg tttccgacgc ccattcagag caatcgacct cctgcgcgac ggacacggcg
     7201 cctgggagca ggaatgagag cacgactgct actgccgcca tgatccgcaa tggaccagtt
     7261 cgaccggtat gcgttgacat acgacctcct tgcatgaaaa accgatgccg atagccgcat
     7321 gcatcccgaa tcaggataca cgtcaccacg ggacgaacgt gcgcgccgtt cgggtggtgc
     7381 cggtccggtc ccggacgccc acactcatca atggcgtagg ggtcggtcgc cgcatgcggc
     7441 gaccttcggc attcgcggcg cgtcgtcgtg tcgtggctta cgtccttgat gctagcagat
     7501 ggaccacgtc atgtcatgcg tctggcgtgg ctcagcttcc ctgctcgacg gtctccatcg
     7561 cgacgcggag cgcgatcgtg agctcatcag tatccgggac ttgaggccag cgggttatca
     7621 ggcggggtgc ggcaccggct gtgcggccgt ttagcgcgac gatatccgcc ggaccagccg
     7681 tgaatgcccg ggcagcgact gcgtatccct gcccattacg ctgccatgcc aatgtcatcg
     7741 ccgtgccggc gtcgaagcgc tggatcccgg tctcgatcct gacgggccag ccagactgca
     7801 ctcgctcggt cgcctcccgg agcacgcgct cacggggggt gagtgcccgc acgtcgatct
     7861 tgcccgggcg gacgccgccc aggaagccgc gctccatgcc ggcggggacg tccgtcagca
     7921 tggccagccg tgcctttatc gcgtcattgg tgtagagcag gtatccaagc gccaccatgc
     7981 cgaactgcat tgcgacgatg agcgccgtca gcagcggcac gcccgagccg tacatctggc
     8041 gccagtcacc gttcatgccg gaaacgaggt cgagcaatgg gtagacgatg aaggcgttgg
     8101 cggcgctgat gatcgcgaac tggaccagca gctcgttgat ggccgcgcgg aacggcctgg
     8161 ggttgaagaa gacgattgcc agggcgccga ggctgaggat gaagttgacg atcgtgcccg
     8221 cagcggctac gacgaagagc tcgacctcgc tgaagccgaa cgggatgaac gagacatatc
     8281 ccgcgaagac gtaatacccg aagtccacga cctcgccacc gtagccccag acggccacgg
     8341 cgtgcccgag ctcgtgcaac gcgaccgaga tcggcaccag cacgaagaag gccgcctcgt
     8401 cgaccagctg gcgatcctgc acgctgaagc ggcggtccca caccgggccc cagttggcga
     8461 tcatgcgcga gaccgagcgg atgccgatga cgacgtagat caggctgatc agggtgaacg
     8521 ggccaaggcc catgtgtcct ccagtttgcg cgtttcgcgt gctcagttcg cgcgctcagt
     8581 cggccggctt ggcttccggc gcgtgcgacg ccggctcggc ggcgcgcatg tggcgcgtcg
     8641 acaagatctg gaacgctgcg gcagcgagta tcagtgtgcc acccatgacg agggaggtcg
     8701 ccacgccgaa ctgcccggtt acccagccgg caatcagcgc gccgatcggg atcgagcccg
     8761 cgaagacggt catgtagacg gccatgacgc gaccgcgcag ctcgtcactg gcggcggtct
     8821 ggacgagcgt gttagcggtt gacatggtgt tggtcgaggc aaagccgacg agcgccagcg
     8881 ccgcggcacc gagcatcacg gcggtcccgt tggcggcggc gagcgcgagc acgagttccg
     8941 cgctgcccat gaggagagcg gcggcgatca tccgtcggcg gctcggaccc tgaccgaaga
     9001 aggcaagcga gagcgcgccg atcagcgacc ccgcgcccat cgccgcgaag agcgtgccgt
     9061 aggcgctcga gcccgcagcg aactcgtcgg atgcgagcac cgggatccaa atattgaagt
     9121 tcatgccgaa gatgccgatg gtgccgacca tcagcacggt acgcgagatg agggggtcac
     9181 gcctgacata cgcgagaccg tcccgcaggc gctggagcgg cgattcctcc gatgtgccgg
     9241 tgatcggcct ggtgcgcatg agcagcaggc cggcgagcac ggcgatgaag ctggctgcgt
     9301 tgatcgcgaa gaggggcccc ggtccgacca cagcgagcag ggccccggcg agtgccgggc
     9361 cgacgatgcg gccggtgttg aagagcgccg cgttgagcgc gatcgcgttc atcaggtcat
     9421 ccttctccac catctcggag acgaacgcct ggcgcgtggg catatcgagc gctctggcca
     9481 tgccgagccc ggttgcgagc acatagacct gccagaggag gacgcggtcg gtaatcacga
     9541 gcactgccag gatcgccgag agcaccatca tcgccgactg ggtggtcagc agaatccggc
     9601 gcttgggaaa gcggtcggcg atcacccctg ccgggatacc gagcagcagc acgggaagga
     9661 actggaggac gttgacgaga ccgagctgga acggagtcgc ccccaggatc tcgtagacga
     9721 gccacgatag cgcgaggctc tgcatccagg tgccggtcac ggaaaccagc tggccaagcc
     9781 aaaagagacg gtagttgcgg taggtgaggg caacaaaccc cttgcggagc ccgctgaagc
     9841 gcgagcggcg cccggggtgt tggcgtgggc tgcctgcggc tggcgatgcg gtgtccggtg
     9901 gatcgggcgg catggaacag ctttgcgacg aggggcgagg tccggcacgg cgaccctgtc
     9961 gatcggtcag ggcatgccga gtccaagtat cgcatgcact ggcaagcgcg atgtgatcgg
    10021 ggctcaggac tgcgggtggg gatgcttcac gagcagtacg ggtacgtagg cgtcccgcaa
    10081 cacgtcctcg gcaatgctgc cgagcaccca acgcctgatc ccgctgcggc cgtgcgagcc
    10141 catcacgatc atgtccccgg actcgacctc gtcgaggatg cttcgcgcga tcggtccgat
    10201 caggacctcg cgcgtggccg tcaagccttc gccctcgaga cgtttggtga tctcgtcgag
    10261 atagctcccc gcctcggcgg attcctgctc gaacatctcg ccgagcacgg tgtagttgaa
    10321 ggcggctgcc gagccaccga cctgctccag gacgtgggtg tcgacgaccc ggacgaggtg
    10381 gaccggcaga tcgaacgctg ttgcctgccg gatcgcctcg tcgagggccg cctcggaggt
    10441 cctggagccg tcgaggggga tcagaatacg actggtcatg acattgctcc tccacgtgcg
    10501 cgatgcgacg ccccggctgg ggcgggcatc gtatccttaa gtgcattctc gcgtgggcga
    10561 tgcgcgccgt cacccctgtt gaggtgcagg gtcgagtcgt tttgcgggat gcgtcgtatc
    10621 gtcatgcgac acccc
//
LOCUS       DASYWL010000324         4336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_316922, whole genome shotgun sequence.
ACCESSION   DASYWL010000324 DASYWL010000000
VERSION     DASYWL010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316922"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /gene="ffs"
                     /locus_tag="VGR22_11430"
     ncRNA           <1..212
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VGR22_11430"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(366..815)
                     /locus_tag="VGR22_11435"
     CDS             complement(366..815)
                     /locus_tag="VGR22_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129219.1"
                     /translation="MDNPFSWDYLTSTPASGEILEPWSMLILVLCAIAMLVGYALYTR
                     PGIMYSRHLLRASSTRRWGSILMWIAITGYFFFIIRWLQINPFTFGNRIWLFLVLLAL
                     ILAAVLMWLQVRSEAPVLNRQRRAQQEAVARGIHTRRPPRISRNRRR"
     gene            complement(859..1557)
                     /locus_tag="VGR22_11440"
     CDS             complement(859..1557)
                     /locus_tag="VGR22_11440"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEV2129220.1"
                     /translation="MDVTLRIKRFNPETDQKPHFEEYTVDVEGTDRVLDALNQIKWKH
                     DGTLTYRRSCAHGICGSDAMRINGRNRLACKVLMKDLGKKVTIEPLIGFKIEKDLLVD
                     METFFNQYKSIEPYLIADDAPSASERLQSPEDRARFDDTTKCILCASCTSSCPITWTS
                     DDYVGPAAIVNAHRFIFDSRDKGAGRRLQILNQQSGVWRCRTVFNCTDACPRDIKVTE
                     AIMQVQRALLNEQP"
     gene            complement(1593..3323)
                     /gene="sdhA"
                     /locus_tag="VGR22_11445"
     CDS             complement(1593..3323)
                     /gene="sdhA"
                     /locus_tag="VGR22_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEV2129221.1"
                     /translation="MAYHRYDAVIVGAGGAGLMAAIQMAGHANIAVVSKLYPPRSHTG
                     AAQGGISAALGNTEEDHWEWHMFDTVKGGDYLVDQDAAEVLAREAIDAVIELEHMGLP
                     FNRTPQGKIDQRRFGGHTRNFGEAAVRRACYAADRTGHMIIQTLYQQGIKHNVNFFDE
                     FHLLDVLFTDDGEACGVVVYEIKTGELHTVHAKTVLIATGGFGRVFKITSMALAGTGE
                     GVAIPYRRGIPLMDMEFFQFHPTGLYKLGILLSEAARGEGGILRNSEGEAFAERYAPT
                     LKDLAPRDMVSRFIYQEVKEGRGIEGKDYVLLDLSHLPPEVIDQKLPDVTDFARVYLG
                     VEPKEEGVPIQPTAHYAMGGIPTNVNGQVIHDPDGSIVPGFYSAGESACVSVHGANRL
                     GTNSLVDLVVFGRRAGRDMLRSVRQNDFVELPKNPEDYARSQVETLLSRNTGERAVNI
                     RAEMQEVMMDKVSVVRNEEFLLDAQAKIAELKDRYQHICIQDHGTTYNTDLLEALELG
                     AMLDLAETTVYGALARTESRGAHYREDYESRDDTEWLAHSMIYHTDAGLELKKKPVTI
                     TRFEPKERKY"
     gene            3566..>4336
                     /locus_tag="VGR22_11450"
     CDS             3566..>4336
                     /locus_tag="VGR22_11450"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV2129222.1"
                     /translation="MIYLTSGFRDSVASAAERAREQIAELTTRLTEVPAPTGDEGDRA
                     AVLARILEEREFDNVHIDDLHDVTAVIRGKRSDRCLLLAAHTDTVFPRSVSITVTREG
                     DRLKAPGVGDNTISVAALVQLKTVLAELGIEPDVDIMVTGNVGEEGLGDLRGMRAVMD
                     ANPDIGAAIAVEGHALGRITHQGVGSRRLEVTITGPGGHSWGDFGRPSAIHAAARLVA
                     HLDEIALPTNPKTALNVGILEGGISVNTIAPESRMVIDM"
BASE COUNT          749 a         1305 c         1443 g          839 t
ORIGIN      
        1 gaggttgaat tccctctcga ggtacggctt ttcaggactg cggccgtggc ggggtgttga
       61 agcccgggtc ccgcacggcg gggaactgcg aatcgggtca ggaccggaag gtagcagacc
      121 taagcaggat tccccgggtg atgtggggac acccgggtgg agccaacgca cggtcgcaag
      181 ctggggagtc cgtatcgaca gagggtgcac ggcattctct agcaatcagg acagaaacgg
      241 ggaggtgata tcacctcgcc cgtttttcgt gccaacgcgt tgtcgccggt taggccctcc
      301 ctggcgttgg gagatatcgg actgaccact actgcccaag tcgatcctgt ggtgacgaca
      361 actggctagc ggcggcggtt gcggctgatg cggggtggac ggcgggtgtg gatcccccta
      421 gccacagctt cctgctgcgc ccggcgctgc ctgttgagca cgggggcctc ggaccggacc
      481 tgcagccaca tcaggacggc cgcgaggatg agcgcgagca gcacgaggaa cagccagatt
      541 cggttgccga aagtaaacgg gttgatctgc agccaccgaa tgatgaagaa aaagtagccg
      601 gtgatggcaa tccacatcag gatcgatccc caccggcgtg tgctcgaagc gcgcagcaga
      661 tggcgggagt acatgatgcc gggtcgggtg tagagcgcgt atccaacgag catcgcgatt
      721 gcgcacagca cgagaatgag catcgaccag ggctcgagaa tctctcccga tgccggcgtg
      781 ctggtcaggt agtcccacga aaacggattg tccatgtcga cagcggcctc cgcacggtcg
      841 catctgccgg gtcagcgatc agggctgctc gttgagcaac gcgcgctgaa cctgcatgat
      901 cgcctcggtt accttgatat cgcgcggaca ggcgtccgtg cagttgaaca ctgtgcggca
      961 gcgccagacg cccgactgct ggttgaggat ctgcaaccgg cgcccggcac ccttatcgcg
     1021 gctgtcgaag atgaaccggt gcgcgtttac gatcgcggcg gggccgacgt agtcatcgct
     1081 ggtccaggtt atcgggcagg acgaggtgca tgatgcgcag aggatgcact tcgtcgtgtc
     1141 gtcgaatcgg gcccgatcct ctggcgactg gagtcgctcg ctggcgcttg gggcgtcatc
     1201 agcaatgaga tagggctcga tcgacttgta ctggttaaag aaggtctcca tgtcaacgag
     1261 caggtccttc tcgatcttga agccgatgag cggctcgatc gtgaccttct tgccgaggtc
     1321 cttcatcagc accttgcagg cgagccggtt gcggccattg atgcgcattg cgtccgagcc
     1381 gcagataccg tgggcgcagg accgccgata ggtgagggtg ccgtcgtgct tccacttgat
     1441 ctggttgagg gcgtccagca cgcggtcggt gccctcgacg tccacggtgt actcctcaaa
     1501 gtgcggcttc tggtccgtct ccgggttgaa gcgcttgatt cgcagtgtga catccacagt
     1561 gaagtctctc cttgccggcg gccggctgga ccctagtact tgcgttcctt cggctcgaac
     1621 cgggtgatcg tgaccggctt cttcttgagc tccagtccgg catcggtgtg gtagatcatg
     1681 ctgtgcgcga gccattcggt gtcatcgcga gactcgtagt cctcgcggta gtgcgccccg
     1741 cgactctcgg tgcgggccag cgcgccgtac acggtcgtct cggcaagatc gagcatggcg
     1801 ccaagctcca gcgcctcgag cagatccgtg ttgtaggtgg tgccgtgatc ctggatgcag
     1861 atgtgctggt agcgatcctt gagctcggcg attttcgcct gcgcatccag caggaactcc
     1921 tcgtttcgga cgacggaaac cttgtccatc atcacttcct gcatctcggc gcggatgttg
     1981 accgcacgtt cgccggtgtt gcggctgagg agcgtctcca cctgtgaccg ggcgtagtct
     2041 tcggggttct tcggaagctc gacgaagtcg ttctgcctga cggagcggag catgtcgcgg
     2101 ccggcacggc gcccgaacac gacaaggtca acgagcgagt tggtgccgag gcgattcgct
     2161 ccgtgcacgg agacgcaggc ggactcgccg gcggagtaga agccggggac gatgctgccg
     2221 tccggatcat ggattacctg tccgttgaca ttggtcggga tgccgcccat cgcgtagtgg
     2281 gcggttggtt ggatggggac accctcctcc ttcggctcca ctccgaggta gacgcgggcg
     2341 aagtcggtga cgtccggcag cttctggtca atcacctcag gcggaaggtg cgagagatca
     2401 agcagcacgt agtccttgcc ctcgattcct cgcccctcct tgacctcctg atagatgaac
     2461 cgcgagacca tgtcgcgcgg cgcgaggtcc ttcagggtcg gggcgtagcg ttcggcgaag
     2521 gcttcgccct cgctgtttcg caggatgccg ccctcaccgc gtgccgcctc ggagagcagg
     2581 atgccgagct tgtagagtcc cgttggatgg aactggaaga attccatatc catcagcggg
     2641 atgccgcgcc ggtatgggat ggcgacgccc tcgccggtgc cggccagcgc catcgaggtg
     2701 atcttgaaga ctcggccgaa gccaccggtg gcgatcagca ctgttttggc gtggacggtg
     2761 tgcaattcgc cggtcttgat ctcgtagacg acaacaccgc acgcctcgcc gtcgtcggtg
     2821 aagagcacat ccagcaggtg gaactcgtcg aagaagttga cgttgtgctt gattccctgc
     2881 tgatagaggg tctggatgat catgtggccg gtgcggtcgg ccgcgtagca ggcgcgccgc
     2941 acggccgctt ccccaaagtt acgcgtgtgg ccgccgaagc ggcgctgatc aatcttgcct
     3001 tggggtgtgc ggttgaacgg aagccccatg tgctcgagct cgatcaccgc gtcgatggcc
     3061 tcgcgtgcca acacctcggc cgcgtcctgg tccacgaggt agtcgccacc cttgacggta
     3121 tcgaacatgt gccattccca atggtcctcc tcggtgttcc cgagagcggc gctgattccg
     3181 ccttgggcgg cgcccgtgtg tgagcgtggc gggtacagct tgctgacaac agcgatgttg
     3241 gcatggccag ccatctggat cgcagccatc aggccggctc cgcccgcacc cacgatcacg
     3301 gcgtcatacc ggtgataggc catccatgaa ctccttcggt gtgtgtgcct gcgtggctgc
     3361 catgcaggag cacctgtcgg tgcgatgttt ccgtatcgtc gcaattgtaa cgcgcggtcc
     3421 tgccttcgtg gtggcgattg tgccgcaatc gctggcaccc cttgccgggc gagtctgaag
     3481 ggcgggcccg ctccttcggt atactcaacc atcgacccgg cgcggcgccg tgcggcgtcg
     3541 ccagctgaat ccggagcatc cttccgtgat ctacctgaca tcaggattcc gcgattctgt
     3601 cgcgtccgct gccgagcgtg ctcgtgaaca gatcgcggag ctcaccacac ggctcaccga
     3661 ggtgccggcg ccgacaggcg acgagggtga ccgcgcggca gtgctggctc gcatccttga
     3721 ggaacgtgaa ttcgacaacg tgcacatcga cgatctccac gacgtcacgg ccgtcatccg
     3781 tggcaagcga tccgatcgct gtctcctgct ggcggcgcac acagataccg tcttccctcg
     3841 ctcggtgagc atcacagtca cgcgcgaggg ggatcggctc aaggcgcccg gggtcggcga
     3901 caacacgatc agcgtggcgg ctctggttca gttaaagacg gtgctggcgg aactcggcat
     3961 cgaaccggat gtcgacatca tggtgacggg caatgtcggt gaggaaggac taggcgacct
     4021 gcgtggcatg cgtgcggtaa tggacgccaa cccggacatc ggcgctgcga tcgctgtcga
     4081 ggggcacgcg ctcgggcgaa tcacccatca gggggtgggc agccgccggc tcgaggtaac
     4141 gatcaccggt cccggtggcc acagctgggg tgacttcggg cgcccgagcg ccatccacgc
     4201 tgcagcgcgg ctggtggcac atctggacga gatagcgttg ccgacgaacc ccaagaccgc
     4261 cctcaatgtc ggtatccttg agggcggcat cagtgtcaac accatcgcgc ccgagtcgcg
     4321 catggtgatc gacatg
//
LOCUS       DASYWL010000325        10351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_373356, whole genome shotgun sequence.
ACCESSION   DASYWL010000325 DASYWL010000000
VERSION     DASYWL010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10351
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373356"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..839
                     /locus_tag="VGR22_11455"
     CDS             159..839
                     /locus_tag="VGR22_11455"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEV2129223.1"
                     /translation="MNTPYPGANGAFRSPGEVVALRWRRHTPADLIVPVRVVEHDASR
                     TILYLAAGSPIKVHADAEGNPLPRSMPLMERERKIASLHDSQWTRTHVLMIHQPHRLG
                     AVWLFWSEIDWSFRNYYVNLQAPLEPSVPGFDTEDFLLDIVVAPDLSFRWKDEDEFAI
                     AIEHELISPVLLHAVRAEGRRFIDEAQSRQWPFAQGLETWRPEPGWNVPDLPENWADG
                     LDLPGSGN"
     gene            1125..2384
                     /locus_tag="VGR22_11460"
     CDS             1125..2384
                     /locus_tag="VGR22_11460"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2129224.1"
                     /translation="MKRIGIVGYGSRIRRMAEELERFGRDIAVVAAVDPAEQDLRERY
                     PERMAGVIFYPDATAMLDEAGLDGVMIGTRCNIHTELAIQVLERDLPLYLEKPVSIEE
                     EQLLRLHAASQRREPKVVVGFPLRLSTLAREAKAILDSGTIGTIENVQAINNVPFYGS
                     NYYHGWMRDDSLTGGLWLQKATHDLDYLTHLVGQLPERIVAVESKTVFTGDMPAGLSC
                     VDCPHYTTCPESQYNLFTLQGIMDTLENEEDWWGGQWRCSFAVDTGNHDAATAILQYP
                     SGAHLTYTQNFYARRTAAKRGASFIGYKGTLEFDWYRGEIVVHHHLSGRVERHDYSGR
                     EIAHTGRPGGGSSGGGAHFGGDMELARDFLNLLFGEGESRATLEDGLISAQLCLMAKR
                     SCETNAFQTYAPLSMRQRELANAGVRR"
     gene            2502..3434
                     /locus_tag="VGR22_11465"
     CDS             2502..3434
                     /locus_tag="VGR22_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129225.1"
                     /translation="MGGLILPHGIRAQGDNQLPQATEPAPTESDTAETAPAQTASQPQ
                     GDGTGGIRMSEGLLPDYRLLFYYGFPENELMGILGEYSPEENLQLLREQVAEYEAVDS
                     TRPWKIGFELIASVAQGTPQGDNSYVADTDGDWLDLYTEFTAEHDMVLLLDVQMGRKE
                     PKEDYQGLERWLRYDHVHLGIDPEFKMWGDEIPGQDLGHVTAEQVTEAQQWLVDVADK
                     YGTSRKMLVVHQFHVYMIEDKDQIKPMAGVDLVIDEDGWGPPDMKMDTYEVVITQQPI
                     EYHGIKLFYQQDDPLMTPAEVMALEPTPDVVIYQ"
     gene            complement(3533..4246)
                     /gene="thpR"
                     /locus_tag="VGR22_11470"
     CDS             complement(3533..4246)
                     /gene="thpR"
                     /locus_tag="VGR22_11470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEV2129226.1"
                     /translation="MTSDEQRNQPRRGPSKRQQYRKERPAPETVDTDWRLFIAIPMPQ
                     EVRDRLRRLIDRLEPEGWPMRWVDPDNAHLTLHFLGDTPPEQAEILRLALGPIVGQHP
                     AFDLRTAGLGVFPKLKRPRVLWLGVWGPTHRLESIYNNLGDALDNLQFPIEERPFSPH
                     ITLGRGRGGSNVPIRDLPEKLRAAIDTMAAEGLADPQNGLPLPIEEVLLMRSHLNREG
                     PRYEVIERFPLAPRDADGD"
     gene            4346..5212
                     /gene="pheA"
                     /locus_tag="VGR22_11475"
     CDS             4346..5212
                     /gene="pheA"
                     /locus_tag="VGR22_11475"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV2129227.1"
                     /translation="MSIAFLGPRGTYSEEAAVTYGGLNAELIPSSSIPACAEAVERGE
                     TGAAVLPIENSIEGGVSTTLDLLIHETDLRICHEVVVPIRHVMVAKEEIPISEVRTVM
                     SHPQALGQCRRFLDREMPNAEQVAALSTAGAVQAVVEGSDLAVVAVGPAPAQQLYGGV
                     IIAENIQGNPSNLTRFVVLAREDAAPTGDDKTSIGLTADADEPGVLEKIIRPLAEAGI
                     NLTRLESRPTKGWLGTYVFLIDFEGHRADPNVREVLSAMEQYAETLKVFGSYPRFPIE
                     TLRDVMSVKSLH"
     gene            5385..6194
                     /locus_tag="VGR22_11480"
     CDS             5385..6194
                     /locus_tag="VGR22_11480"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HEV2129228.1"
                     /translation="MTILIDDTTATAFLTAPVREGWFEGDPPIEVRADLRAADVTEDD
                     VALLPLPEATLLTRTHVIDRSVAIVHDGVGMVAMWTPARPDEIEGPVVCLRDVGASGE
                     ALVRALLKPFFGIEATKIAPRDDPPADAQVIITEGAEALAPSESGFREDLARSWFIMT
                     GKPFVSHVTVIGVRALARDGDEQLAALKKAVETGWERRRDVRRMIREQSGIESEPLAE
                     VTNRMRFLMEPDDQEPARMLAERGTWGTDYGRTLPAYRDQLRRVESEGETA"
     gene            6191..7372
                     /locus_tag="VGR22_11485"
     CDS             6191..7372
                     /locus_tag="VGR22_11485"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HEV2129229.1"
                     /translation="MSDGTKRRGFGTRAVHAGEAPDPVTGAAGVPIYQNATFAFRSAG
                     QIDRFNNGEVPHYIYSRYGNPTVRCLELKLADLEGAEDVLATASGMAAVTTVAFHYVA
                     GGGHLVVSDSLYEATRDFFCRGIGDYGATATAVDMTDFDAVRAAITPDTRALHVEPFS
                     NPTLKIADIDTLAAIAHEAGVDLIVDNTFLSPALYNPIEHGADIVIHSATKYLSGHSN
                     VIGGAIAGKAGATVAMRERLAQLGGIMAPQSGWILLNGVKTLALRMAQHCENGLAVAK
                     VMAEHPAVETVHYPGLPSHPGHERAAEMTSGRFSGMVSVRLVEHERARNVFLDALTIP
                     LKAVSLGDVASLVWPFDEDGVIRLSVGIEEAGDLVADVTQALDAVSAQIVTNLGAPAS
                     S"
     gene            complement(7497..8189)
                     /gene="moaD"
                     /locus_tag="VGR22_11490"
     CDS             complement(7497..8189)
                     /gene="moaD"
                     /locus_tag="VGR22_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HEV2129230.1"
                     /translation="MNITVRFFAMFREQLGTGEKTITIPEGSTAREGFDTALADAPAL
                     QGLADSVMLMVNQEYVDPDHVLQGGDELAIIPPVSGGDHRKHFAVTEHELNPRAVEAL
                     VAGPGEGAIVTFIGAVRDHARGRIVTLLEYEAYDAAAEKMLARVGDEARARWPDVTMA
                     ITHRTGALKPGEASVVIACASPHRDAAYEASAFAISRIKEIVPIWKKEHYEDGSTWVG
                     SEADYQRLTGRL"
     gene            complement(8269..8499)
                     /locus_tag="VGR22_11495"
     CDS             complement(8269..8499)
                     /locus_tag="VGR22_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129231.1"
                     /translation="MANQNQEFAVSDVEYNVITTMSNLLQSEEVLMKYATDAREAGDS
                     ELEQMFHDLRRHNGEVAQKLYQKLGQLASKAS"
     gene            complement(8624..>10351)
                     /locus_tag="VGR22_11500"
     CDS             complement(8624..>10351)
                     /locus_tag="VGR22_11500"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEV2129232.1"
                     /translation="IAFSSKRGNDDAKTQLWVMPTDGGEPIRVTSFDNGVLEHDWALD
                     SQRLVVVSPVEMKPEHAETEADVRVITSEHYKSNGRGFVDHRYPQVFVVDRTEPDAEP
                     LQLTEGWFLHRSPAWSPDGREIAFATNRNPDWDTSRISDIWTVRAHGGEPRRLTDGTG
                     SYDSPVWSPDGTRLPFTGEAELDHVYRNTRLWMILAAGDDAIDLSGDINRSIGDSSMS
                     HPTGDLSGSPIRWTLDSNAVDALVDDRGSTRIVRYPLDKGDVTALTPLGMHVMAFDHL
                     GNHRLAITVADATTPAELATITRKQFARITDFYADWVADVAVAMPEELIVDVNGTAVQ
                     GWLLRPRNHTPERNTPLIINIHGGPHGQYASAFFHELQLYAARGCGLLYINARGSVGY
                     GEQFAAAVTGAWGEADTPDHEAMLEHVLAQDGWDRDRLGVTGGSYGGFMTNWLLGHTD
                     QFRAGVTDRSISNMASMYGTDDIAMVTLDPELGTPWDHPERYWKLSPLRTVANITAPL
                     LIVHSEEDYRCPMEQAEQLFIALKRLGREVEFVRYQGENHGLSRSGKPKNRVDRLQRT
                     LGWFERHLA"
BASE COUNT         1775 a         3324 c         3429 g         1823 t
ORIGIN      
        1 cgagctcggt gtaccgccgc tccagctcat tgagatttac gctgatactc tgcatcgtgg
       61 cagccatgtg ggatgcttct ccgcgaggaa acgaggcctg ctgggtccgg gaccgcaact
      121 cctgaccctc aagtatagcc cggagcaccc atccccttat gaacaccccg taccccggag
      181 cgaacggcgc ctttcggtcc cccggcgagg ttgtcgcgct tcgctggcgt cgccacaccc
      241 cggctgacct gatcgttccg gttcgcgtgg tcgagcacga cgccagccgc accatcctct
      301 acctcgccgc cggcagtccg atcaaggtcc acgccgatgc cgagggcaac ccactgcctc
      361 gctcgatgcc actgatggag cgagagcgga agatcgccag cctgcatgac tcgcagtgga
      421 cgcgcacgca cgtgctgatg atccaccagc cgcaccggct gggagcggtc tggctcttct
      481 ggagcgagat cgactggtcg ttcaggaact actatgtcaa ccttcaggcg ccgctggagc
      541 cctctgtacc gggattcgac accgaggatt tcctgcttga tatcgtcgtt gcgcccgacc
      601 tctcgttccg ctggaaggac gaggacgagt ttgctatcgc cattgagcac gagctgatct
      661 cgccggtgct gctgcacgct gtgcgggccg aggggaggcg cttcatcgac gaggcgcaga
      721 gccgccagtg gcccttcgca caggggctgg agacgtggag gccggagcct ggctggaacg
      781 tgcccgacct gccggaaaac tgggcggacg gcctggacct gcccgggtcc ggcaattgac
      841 agggcttctc tctccgggga gactccccgg ggacattgat ggattgtgcg cattccatcc
      901 tgtgtgagag gacttgcgcg cgctcacttc cgtcacggct gccaccgctg gcgcgggcaa
      961 gtcctcccgc tccagaaaac acatcatctc aacaaaccgg gacgacattg gatgcgctca
     1021 ccatggtgtg tggcggacaa tgatacgcgc atccaagcgc cccctacagt ccaccggatc
     1081 agtgttcacg ggttcagacc agcgcaccag aaggaaccac acttatgaaa cgcatcggaa
     1141 tcgtcggata cggtagccgc atcaggcgca tggccgagga gttggagagg ttcggccgtg
     1201 acatcgctgt ggtggcggcg gttgaccccg ccgagcagga cctgcgcgag cgctaccccg
     1261 agcggatggc cggtgtcatc ttctacccgg acgcgacggc catgctcgac gaggccgggc
     1321 tcgacggcgt tatgatcggc actcgctgca acatccatac cgaactcgcc atccaggtgc
     1381 tcgagcgcga cctgccgctc tacctcgaaa agccggtctc aatcgaggag gagcagctcc
     1441 tgcgtctcca cgccgcctca cagcggcgcg agccgaaggt ggtggtcggg tttccgctgc
     1501 gcctgtccac cctcgcgcgg gaggcgaagg caatcctcga cagcggcacg atcggcacga
     1561 tcgagaacgt tcaggcgatc aacaacgtgc cgttctacgg ctcgaactac taccacggct
     1621 ggatgcgcga cgactcgctc accggcgggc tgtggctgca gaaggcgact cacgatctcg
     1681 actacctgac gcacctcgtc gggcagttgc cggagcggat cgttgccgtc gagtcgaaaa
     1741 cggtcttcac cggtgacatg cccgccggtc tttcctgtgt tgactgtccg cactacacga
     1801 cctgcccgga gagccagtac aacctcttca ccctgcaggg catcatggac accctcgaga
     1861 acgaggaaga ctggtggggc ggtcaatggc ggtgcagttt cgcggtcgac accggcaacc
     1921 acgatgctgc gaccgcgatc ctgcagtacc cctccggcgc ccacctgacc tatacgcaga
     1981 acttctatgc tcggcgcacg gcggcgaagc gcggcgcgag cttcatcggg tacaagggca
     2041 cccttgagtt cgactggtac cgaggcgaga tcgtggtgca ccatcacctg agtggtcgcg
     2101 tggagcggca cgactattcc gggcgagaga tcgcccacac cggtcggcca gggggcggat
     2161 ccagcggtgg cggcgcccat ttcggcggcg acatggagct ggccagggat tttctcaacc
     2221 tgctcttcgg agagggcgag agtcgcgcta cgctcgagga tgggctgatc agtgcacaat
     2281 tgtgtctgat ggcgaaacgc tcgtgcgaaa cgaacgcgtt ccagacatac gcaccgctct
     2341 ccatgcgaca gcgggagctt gccaatgccg gcgtgcgccg gtaacggcct gacgcccagt
     2401 gaccgaggca gggtgacgtg aagcaacagc gaccagtcaa gcatccgatg ctacatcggc
     2461 cgatgcgtcg gcgccagttc ttgtccgcag gtgccgccgc catgggcgga ctgatcctgc
     2521 cgcacggtat ccgcgcccaa ggcgacaacc aactcccaca ggcgaccgaa cccgcgccca
     2581 cagagagcga tacagcggag acggctcccg cgcaaactgc ctcgcagccg cagggcgacg
     2641 gcaccggcgg cattcggatg agtgagggcc tcctgccgga ctaccggctg ctgttctact
     2701 acggattccc cgagaacgag ctgatgggca tcctcggcga gtactcaccc gaggagaacc
     2761 tgcagctgct gcgcgagcag gtggctgagt acgaggcggt cgacagcacc cgcccatgga
     2821 agatcggctt cgagctgatc gcctcggtcg cgcagggcac gccgcagggc gacaacagct
     2881 acgttgcgga cacggacggc gactggctcg atctctacac cgagttcacc gcagagcatg
     2941 acatggtgct gctgctcgat gtccagatgg gccgcaagga gccaaaggag gattaccagg
     3001 ggctcgagcg ctggctgcgg tacgaccacg tccacctcgg catcgacccc gagttcaaga
     3061 tgtggggcga cgagatcccg gggcaggacc tgggccacgt caccgccgag caggtgacgg
     3121 aggcacagca gtggctggtc gacgtcgccg acaagtacgg cacctcgcgg aagatgctgg
     3181 tggtgcacca gttccatgtc tacatgatcg aggacaagga ccagatcaag ccgatggccg
     3241 gtgtcgacct ggtgattgac gaggacggtt gggggccgcc ggatatgaag atggacacct
     3301 acgaggtcgt catcacccag caaccgatcg agtaccacgg catcaagctc ttctaccagc
     3361 aggacgaccc actgatgacc ccggcggagg tcatggcgct cgagcccact cccgacgtcg
     3421 tcatctacca gtaagccgat cgcccaccaa cctgtcattc tgagccgagc cctgccggaa
     3481 ggcggggctc gtgtcgaaga atcttcgcgc gaaacgcgct gtcgcagggc agtcagtccc
     3541 cgtcggcgtc tcgcggggcg aggggaaacc gctcgatgac ctcgtaccgc ggtccctccc
     3601 ggttcaggtg gcttcgcatg agcagcacct cctcgatcgg cagcggcagg ccgttctgcg
     3661 ggtcggccag gccctcggcg gccatcgtgt cgatcgccgc tcgcagcttc tctggaagat
     3721 cgcgaatcgg cacattgctg ccgccgcgcc cccgaccaag cgtgatgtgc gggctgaacg
     3781 gccgttcctc gatcgggaac tgcaggttgt ccagcgcgtc cccgaggttg ttgtagatcg
     3841 attccagccg gtgcgttggc ccccacaccc cgagccacag cacccgcggg cgcttcagct
     3901 tcggaaagac gccgagcccc gccgttcgca ggtcgaatgc cggatgctgg ccgacgatcg
     3961 gcccgagcgc cagccgcagg atctccgcct gctccggcgg cgtgtcgccg aggaagtgca
     4021 gggtgaggtg ggcgttgtcg ggatcgaccc accgcatggg ccagccctcc ggctcgagcc
     4081 ggtcaatcag cctgcgcagc cggtcgcgca cctcttgcgg catgggtatg gcgatgaaca
     4141 gtcgccagtc ggtgtcgacc gtttccggtg ccgggcgctc cttgcggtat tgctggcgct
     4201 tgcttgggcc gcgcctgggt tggttgcgtt gctcgtcgct cgtcatgggt ggagtatacg
     4261 gggcggggag tgcctgccac agccttgcat ctaggggtaa ggttaggccc ttgacacgag
     4321 cgctggcgag gtggggtagg gcagcatgtc gatagccttt ctgggaccgc ggggaaccta
     4381 cagcgaggag gcagccgtca cgtacggtgg tctcaatgcg gagctgatcc cgtcctccag
     4441 cataccggcg tgcgcggagg cggtcgaaag gggcgagact ggcgccgcgg tgctgccgat
     4501 cgagaactcg atcgagggcg gtgtgagcac cacgctcgac ctgctcatcc acgagaccga
     4561 tctccgcatc tgtcacgagg tggtggtgcc gatccgccat gtcatggtcg cgaaggagga
     4621 gattcccatc tcggaggtcc gcacggtgat gtcccaccca caggcgctcg gccagtgccg
     4681 ccggttcctg gaccgagaga tgccgaatgc ggaacaggtg gccgcgctct cgacggcggg
     4741 cgcggtgcag gcggtggtcg aggggagtga cttggcggtt gtggcggttg gcccggcccc
     4801 ggcgcagcag ctctacggtg gggtgatcat tgccgagaac atccagggca acccgagcaa
     4861 cctgacgcgc ttcgttgtgc tcgcgcggga ggacgctgcg ccgaccggtg acgacaagac
     4921 gtcgatcggg ctgaccgccg atgccgacga gccgggcgtg ctggagaaga tcatccggcc
     4981 gctcgccgag gccgggatca acctcacgcg cctggagtcg cgcccgacga agggatggct
     5041 ggggacctat gtcttcctga ttgacttcga agggcaccgt gccgatccga atgtgcgcga
     5101 ggtgctctcg gcgatggagc agtacgccga gacgctcaag gtcttcggct cctacccgcg
     5161 cttcccgatc gagacgcttc gcgatgtgat gagcgtgaag agcctgcact gagcgaagcg
     5221 aatgcgtatc gaagcatctc gtgttcactg accttcatcg tcgcactgag agtcctcgac
     5281 gattgcgatc cttcgactcc ggccttcttc gctcaggatg gcgccaagtg tgtgattatc
     5341 acctttggca tgacccgatg ggcaggtgag gagatactcg tttcatgacc atcctgatcg
     5401 acgacacgac cgcaaccgcg ttcctcaccg ctccggttcg agagggctgg ttcgagggcg
     5461 atcctcccat cgaggtccgc gcggacctgc gcgccgctga tgtcaccgag gacgatgtcg
     5521 cgctgctgcc gctgcccgag gcgaccctgc tcacccgtac ccacgtcatc gatcgctccg
     5581 tggcgatcgt ccatgacggc gtcggcatgg ttgcgatgtg gacgccggcc cgtccggacg
     5641 agatcgaggg cccggtggtt tgcctcaggg atgtgggggc gtccggcgag gcgctcgtgc
     5701 gtgcgttgct gaagcccttt ttcggcatcg aggcgaccaa gattgccccg agggacgacc
     5761 cgcccgccga tgcacaggtc attattaccg agggcgccga ggcgctcgct ccgtccgaga
     5821 gcgggttccg cgaggacctt gcccggagct ggttcatcat gaccggcaag ccgttcgtgt
     5881 cgcacgttac cgtaatcggc gtccgggcgc tcgcccggga cggcgacgag cagctcgccg
     5941 cgctgaagaa ggcggtcgag actggctggg agcgccggcg agatgtccgc cgcatgatcc
     6001 gcgagcagtc aggaatcgaa tccgagccgc tcgccgaggt gaccaatcgc atgcggttct
     6061 tgatggagcc ggacgaccag gagccagcgc gcatgctcgc cgagcgaggt acttggggca
     6121 ccgattacgg ccgcaccttg cccgcatatc gcgatcagct ccgacgcgtg gagagcgaag
     6181 gagagacagc gtgagcgacg ggacgaaacg acggggtttt gggaccaggg cggtgcatgc
     6241 gggcgaagcg ccggacccgg tcaccggtgc cgccggcgtg ccgatctatc agaacgcgac
     6301 cttcgccttt cgatccgcag gccagatcga ccggttcaat aacggcgagg tgccgcacta
     6361 catctactca cgctacggca accccaccgt gcgctgtctg gaactgaagc tggccgatct
     6421 ggaaggggca gaggatgtgc tggcaacggc gtccggcatg gctgcggtta ccaccgtcgc
     6481 gttccactat gtcgcgggcg gcggtcacct ggtggtgtcc gactccctct acgaggcaac
     6541 gcgagacttt ttctgccgcg gcatcggcga ctacggcgcg acggcgaccg ccgtcgacat
     6601 gacggacttc gatgccgtca gggcagccat cacgcccgat acccgcgcgc ttcacgtcga
     6661 gccgttctcc aacccgaccc tgaaaatcgc ggatatcgac acgctggcgg cgattgccca
     6721 tgaggctggt gtcgatctca tcgtcgacaa cacattcctc agcccggcgc tctacaaccc
     6781 gatcgagcat ggcgcggaca tcgtgatcca cagcgccacc aagtacctct ccgggcacag
     6841 caacgtcatc ggtggcgcga ttgcgggcaa ggccggggcc acagtggcga tgcgtgagcg
     6901 gctggcccag ctcggcggga tcatggcgcc gcagtcgggc tggatcctgc tcaatggtgt
     6961 caagacgctc gcactgcgga tggcgcagca ctgcgagaac ggcctagcgg tagcgaaagt
     7021 catggccgag cacccggcgg tcgaaacggt gcactatcca gggctgccat cccacccggg
     7081 tcacgagcgc gcggccgaga tgaccagtgg tcggttcagc ggcatggtgt cggtgcgcct
     7141 ggtcgagcat gagcgtgccc gcaacgtctt tctggatgcg ttgacgatcc cgctgaaggc
     7201 cgtcagtctc ggagatgtgg cgtcgctcgt ctggcccttc gatgaggacg gcgtgatccg
     7261 gctctccgtc ggtattgagg aggctggtga tcttgtcgcc gacgtgaccc aggcgctcga
     7321 tgcggtttct gcccagatcg tgacgaacct gggagcccca gccagctcgt agggacaggt
     7381 cttgccaagg tgtctgcccg ctccttcgaa cacgaccaac tacggccccg taagccagcc
     7441 gtcatgtcgg gcggctcgga atgacgattt ccggtgcccg gagatgcggt tgggttctac
     7501 agcctgccgg tgaggcgttg gtagtccgcc tcgctgccga cccacgtgga gccgtcctcg
     7561 tagtgctcct tcttccagat cggtacgatc tccttgattc gcgagatcgc gaaggcgctc
     7621 gcctcgtagg cggcatcccg gtggggtgac gcacaggcga tcaccacact cgcctcgccg
     7681 ggcttcaacg ccccggttcg gtgcgtgatc gccatcgtca catccggcca ccgcgcccgc
     7741 gcctcgtctc ccacccgtgc cagcatcttc tccgcggcgg cgtcgtaggc ctcgtactcc
     7801 agcagcgtca cgatccgacc gcgcgcgtgg tcgcgcacgg cgccgatgaa ggtgacgatc
     7861 gctccctcgc cgggaccggc gaccagcgcc tcgacagctc gcgggttgag ctcatgctcg
     7921 gtgacggcga agtgctttcg gtggtcgcca ccgctgaccg gtggaatgat cgccagttcg
     7981 tcaccaccct gcagcacgtg gtcggggtcg acgtactcct ggttcaccat gagcatcacg
     8041 gagtcagcca gtccctgcag cgcgggcgca tcagccagcg ctgtgtcgaa gccctcacgg
     8101 gcggtgctgc cttcgggaat ggtgatcgtc ttctcgcctg tgccgagctg ctcgcgaaac
     8161 atggcgaaaa accggacggt gatgttcacg gtacgggcgc cccctccagt atgggaaatg
     8221 gctgaggacg gggacgacct ctcgggtcac ccccgcatgt atggcaggct aggaagcctt
     8281 cgacgcgagt tggccgagct tctggtagag cttctgggcg acctcgccgt tgtggcggcg
     8341 cagatcgtgg aacatctgct ccagttccga gtcacccgcc tccctggcgt cggtggcgta
     8401 cttcatgagc acctcctcgc tctggagcag gttgctcatc gtcgtgatga cattgtattc
     8461 gacgtcgctg acggcgaact cctgattctg gttggccacg gtgaaatcct ccgatcgtca
     8521 aaatgcgaca agcttgggag tctatctcgc aagagccggg ccagactgtt gctcgcgatg
     8581 ttcggcgaca tgaactgtag aggcgaatta tccgccctac cactcatgcc aagtggcgct
     8641 cgaaccagcc gagggtgcgc tgcagtcggt cgacccggtt cttcggcttg ccgctgcgcg
     8701 agagcccgtg gttctcgccc tgataccgca cgaactcgac ctcccgcccg agccgcttga
     8761 gcgcgatgaa gagctgctcg gcctgctcca tcgggcagcg gtaatcctcc tcgctgtgga
     8821 cgatgagcag cggcgccgtg atgttggcga ccgtgcgaag tggcgagagc ttccagtacc
     8881 gctctggatg atcccatggt gttcccaact ccggatcgag ggtcaccatc gcgatgtcgt
     8941 ccgtcccgta catcgaggcc atgttcgata tcgagcgatc cgtgactccc gcccgaaatt
     9001 ggtcggtgtg ccccagcagc cagttggtca tgaacccgcc gtaggagccg ccggtgacgc
     9061 cgagccggtc gcgatcccag ccgtcctgcg cgagaacgtg ttccagcatc gcctcatggt
     9121 cgggcgtgtc cgcctcgccc caggcgccag tgaccgctgc cgcaaactgc tcgccgtagc
     9181 cgacgctgcc gcgcgcgttg atgtagagca gaccgcagcc tcgcgcggcg tagagctgca
     9241 gctcgtggaa gaaggcgctc gcgtactgcc cgtgcgggcc accgtggata ttgatgatca
     9301 gcggcgtgtt gcgctccggc gtgtggttgc gcggacgcaa cagccagccc tgcaccgccg
     9361 tgccgttgac gtcgacgatc agctcctccg gcatcgcgac ggccacatcg gccacccaat
     9421 cggcgtagaa gtcggtgatt cgggcgaact gctttcgggt gatggtcgcc agctcggctg
     9481 gcgttgttgc atcggcgacg gtgatcgcga gccggtgatt gccgagatgg tcgaaggcca
     9541 tcacatgcat gccgagcggc gtcaacgcgg tgacatcgcc cttgtcgagt ggatatcgga
     9601 cgatgcgagt gcttccgcgg tcgtcgacca gcgcgtcgac cgcattgctg tcgagggtcc
     9661 agcggattgg ggagccggag aggtcgccgg tcggatggct catggaagag tcgcctatcg
     9721 agcggttgat atcgccgctg agatcgatcg catcgtcgcc ggcagcgagg atcatccaga
     9781 ggcgagtgtt gcggtagaca tgatcgagtt cggcctcacc agtgaagggg aggcgggtgc
     9841 cgtccggcga ccagaccggc gaatcgtacg accctgtgcc gtcagtgagg cgccgcggct
     9901 caccgccatg cgcccggacg gtccagatgt ccgagatgcg ggaggtgtcc cagtcggggt
     9961 tgcggttggt ggcgaacgcg atctctcgcc cgtccggcga ccacgccggg gagcggtgca
    10021 ggaaccagcc ctcggtcagc tgcagcggtt cggcgtccgg ctcggtacgg tcgacgacga
    10081 agacttgcgg gtagcggtgg tcgacgaagc cgcgcccgtt ggacttgtag tgttcggagg
    10141 tgatcacccg gacgtcggct tcggtctccg cgtgctctgg cttcatctcg accgggctga
    10201 cgacgacgag gcgctggctg tccagcgccc agtcgtgctc gagcacgccg ttgtcgaatg
    10261 aggtcacccg gatcggttcg ccaccgtcgg tcggcattac ccagagctga gtcttcgcgt
    10321 cgtcgttacc gcgctttgag ctgaaggcga t
//
LOCUS       DASYWL010000326        20025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_448479, whole genome shotgun sequence.
ACCESSION   DASYWL010000326 DASYWL010000000
VERSION     DASYWL010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 20025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20025
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448479"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..1901)
                     /gene="ilvD"
                     /locus_tag="VGR22_11505"
     CDS             complement(171..1901)
                     /gene="ilvD"
                     /locus_tag="VGR22_11505"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEV2129233.1"
                     /translation="MSDNGKSSETNGEVDMRFNSRTLYDGNDRAGARAMMKAVGFTDE
                     DLGKPIIGIANTWIETMPCNYNLRRLAEAVKKGVRATGGTPMEFNTVAISDGVTMGTE
                     GMKTSLVSREVIADSVELVGRGHMFDGIIALGACDKTLPGLAMALTRLNVPSFCLYGG
                     TIMPGFWRGKEVTIRSVYEAIGANAAGKMSDEDLKDLENHACPGAGACGGQYTANTMA
                     MAIEFLGMSPLGSASPPAVDPRKEEVGEKGGKLIMDLLRRNVRPRDVLTRAAFENAIA
                     GVCATGGSTNSVLHLLALAREAGIDLDIDDFDRISRRTPLIADMMPGGKYAAADLDKA
                     GGIPLIAQRMAEAGLIDGAAITPTGRTLAQEAATATATEGQDVVHAVADPIKKTGGLH
                     ILKGNLAPDGAVVKLFGYERTYHKGPARVYNSEHEAHEAVLNGKINSGDVVVIRYEGP
                     KGGPGMQEMLAVTAAIVGEGHGDDVALITDGRFSGATKGLMIGHVAPEAQVGGPIAAL
                     QEGDQIVIDAENRVLAVELSDDEIAARLKDWKAPELRYKNGVLAKYAKLVRQADQGAT
                     TLLDEDVRWE"
     gene            2125..3024
                     /locus_tag="VGR22_11510"
     CDS             2125..3024
                     /locus_tag="VGR22_11510"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEV2129234.1"
                     /translation="MPGTPDRLLFGIHTIQTWTWPELRDCWKWFESLGFDSLWLPDHY
                     FPTAGRDKPMFEAWTLLSGLALVTERARIGILVSSNTFRHPALLAKQAVTVDHLSEGR
                     LELGIGAGWFVEEHEVFGLDFPETRTLVDRFAEAVQLIDQYLTHDQTSFEGEHYRLNG
                     AWNRPAPVQQPRPPLVMGAHGPRMLKLVARYADTWNSFAPPEELVRRNQQLTDYCEAI
                     GRDPRTVKRSMFYGVNQSVEEKPWSSTDAFEDYIGRYQAAGMQEFILQPPLPDQFGVV
                     ERVASDILPRLRDQFTPLAGGEV"
     gene            3021..3890
                     /locus_tag="VGR22_11515"
     CDS             3021..3890
                     /locus_tag="VGR22_11515"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV2129235.1"
                     /translation="MTPSPDGVRQFGIQTVQTWTWPEMHQRWAWFEEMGWESLWLPDH
                     FVPTANPPGPMFEAWTLLAALATHTKTARLGVLVSSNTFRHPALLAKQAVTIDHISNG
                     RLELGIGAGWFVEEHEMFGLEFPVARQLVEQYAEAVDLLDRYLGSDQMTFDGEYYHLK
                     DAWNRPAPVQKPRIPLMLGAHGLKMIDIAGQYADSWNSRGTPQEMCERNERMDEACRR
                     HDRDPKSIKRSMLYVVAQMPDERPWDSTDAFEDYVGRFSKAGVQEFIFQPPPADKLDI
                     VERVATDVLPKLR"
     gene            3965..4225
                     /locus_tag="VGR22_11520"
     CDS             3965..4225
                     /locus_tag="VGR22_11520"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEV2129236.1"
                     /translation="MGAELANLLLSIIQILSLLIFARAIFSWFDPGFSSQIGRVLFQI
                     TEPIIAPVRQVLPPMGMLDLSVLVTLILLMILQQLIRSALVG"
     gene            4379..4609
                     /locus_tag="VGR22_11525"
     CDS             4379..4609
                     /locus_tag="VGR22_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129237.1"
                     /translation="MIMRMLLLLVMIAQMALNTVDPSDEEEARENMGGEGFANIVMLL
                     ALLGALLLAVLIVLAILSLTGQFEMGLVEPEV"
     gene            complement(4866..5615)
                     /locus_tag="VGR22_11530"
     CDS             complement(4866..5615)
                     /locus_tag="VGR22_11530"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV2129238.1"
                     /translation="MATPDVPVRRVAIVTGAARGIGAATARQLAADGFSVAVADIDLD
                     AARETAAAIGDRTMGVQLDVTDSESWATAVETVRAALGTVTALVNNAGIAGRSAPAWE
                     LSLDEWHKVIDIDLNGVFYGCRTVLPTMMEEGYGRIVNIASIAGKEGNPNASPYSAAK
                     AGVIGLTKSLAKEVATKGVLINAVTPAVIATPILEQISQEHIDYMTSRIPMGRVGQPE
                     EVANLVSFLCSDRVSFSTGAVFDISGGRATY"
     gene            complement(5747..6982)
                     /gene="fabF"
                     /locus_tag="VGR22_11535"
     CDS             complement(5747..6982)
                     /gene="fabF"
                     /locus_tag="VGR22_11535"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEV2129239.1"
                     /translation="MTRAVVTGLGAVTPLGIGVDTFWEGLTAGRSGIRKIQSFDASGL
                     DVQIAGEVPDFNPADHMDIKKARRMDRFSQFGVAAAREAIEHACLEITDDNRERIGIM
                     MNTGGGGIPTIASEVRVMEERGPKRVGSLVIPLFAPNMASCQVAIMFDIHGPTVTSVA
                     ACAAGVQAFVDAVAMIERGDVEVVITGGTEAAIIPVAIASLSNMRALSRCNEAPERAS
                     RPFDADRDGFVFSEGAAAAILESEEHAARRGATPIAFAHGGYYTSDAYHITAPDPSGR
                     GAATAMRGALRRAELEPTDIDYVAAHATATTIGDIAETNMLKNVYGGHAYNLSISANK
                     SMVGHLLGAAGAVSALTCILAIRDGVIPPTINLDTPDPECDLDYTPNTKREREVRAAV
                     ANGFGFGGQNACAAFTAPE"
     gene            complement(7108..8049)
                     /gene="fabD"
                     /locus_tag="VGR22_11540"
     CDS             complement(7108..8049)
                     /gene="fabD"
                     /locus_tag="VGR22_11540"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEV2129240.1"
                     /translation="MALRDIGNTPIAYIFPGQGSQTVGMGRDLVEQSSEAHRIFQTAD
                     DILGFSLSTLCFEGPADELEDTYNAQPALLTTSTASLAVLRQRAAGGGVALNPIVAAG
                     HSLGQFTALVAADAIDFADALRIVRERGRLMKEAGEKQPGGMAAILGMADAELEGVVR
                     DASSEGVIRVANANCPGQTVISGEIPALERATALAQERGARRVTRLGVSIASHSPLMS
                     AAAEQLSGQLAGVAFRDPEVPVIDNSTTEPLATGDAIRGALGTHMESPVNWTDSVRRM
                     IEMGTETFVELGAGSMLAGLNRRIDRNVPTLGLRDLQ"
     gene            complement(8232..9005)
                     /locus_tag="VGR22_11545"
     CDS             complement(8232..9005)
                     /locus_tag="VGR22_11545"
                     /inference="COORDINATES: protein motif:HMM:NF009389.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV2129241.1"
                     /translation="MTKNLVGRVALVTGATRSIGIGAAICRALAARGAKVAFTSFTAY
                     DAEMYDSDVSEPDRLEAELREIGADATNIPWDLTPADKLPALLDVVEETLGPLSILVN
                     NAAYSTRDGWEALTAEELDRHYQVNARATAMLSVEFCRRFQGESGGRIINFSSGQHLG
                     PMSGELAYAMSKAAIITFTQSLAPAVIGKSITVNAVNPGVTDTGWITDEIREWLLPMM
                     PAGRFGTPEDAARVVAWLASDEAAWLTGQVINSEGGMLR"
     gene            complement(9199..9633)
                     /locus_tag="VGR22_11550"
     CDS             complement(9199..9633)
                     /locus_tag="VGR22_11550"
                     /inference="COORDINATES: protein motif:HMM:NF022279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrdB family protein"
                     /protein_id="HEV2129242.1"
                     /translation="MTDDQAPIACPVPTHDDGAPPMQPALMLAFRFLLELVAFWAIGL
                     AGWQLGDGGLPGGVLTAAMAITAMALWGALTVPDDPARNPTPVIVVPGWARLLVELAI
                     FGVAAWSLWVFVSRTASETFLTAAGIVYIVGWDRIWWMLRQR"
     gene            complement(9639..10919)
                     /locus_tag="VGR22_11555"
     CDS             complement(9639..10919)
                     /locus_tag="VGR22_11555"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEV2129243.1"
                     /translation="MSTASPQRRVVVTGIGAVSSLAPTAEESWQRIRDGETGIRRSEL
                     LDPDKHTCLLHGTVDNNAVQNRFLDRKALRNTSRYSRWAVEAAGEAMLQGGLLDGDLQ
                     TNMDLTTGGAALGTCIGGTYDDLLPAYETALKRDSYARVPPHLHVMFPHNLAAYAIQH
                     RFGLGGPSSTVVTACATGTQAVGDGYRAIRDGHAPMMLAGAAESTLHPLFVAGFEAMR
                     ALVTDSNDNPEKASRPFDATRAGFVLGEGAAMFVLEDLEHAQARSAIVLAEIAGYATS
                     NDAYHPIAPEPHGFGAARAMRAALKDAGMEPDEIDHVNAHAASTTAGDKAEAAAIHEV
                     FGERAKRMPVTSIKGAIGHCMAAAGALEFMSAVRTLTDNCIPPTRNHRNPDPEIGLDV
                     VSEARKAEVDAISKHSFGLGGQNAVVILRRWTGT"
     gene            complement(11029..11829)
                     /locus_tag="VGR22_11560"
     CDS             complement(11029..11829)
                     /locus_tag="VGR22_11560"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV2129244.1"
                     /translation="MTANAITDLLVSVDWLAEHHDDANLLIVDVRPEDQYAQGHIPGA
                     VSLDLYPIKILGSDPEKIEGWVREIEHAFRQIGIDRERHVVFYEDISGTTAARGVWLM
                     HALSIGEGAMLDGGLRAWLAAGNEISTEPVTASQSDVTATLDPALFATADDVLSASND
                     DTDTTIIDTRADLEWMQGTIPGARHVEWIHHLNRDGTIKPIDELRALYTDMGLAPDDD
                     AITFCASGYRAAHTWLVLRMLGHDRVQNYAPSWGEWGLRDDLPVTSTQ"
     gene            complement(11857..12495)
                     /locus_tag="VGR22_11565"
     CDS             complement(11857..12495)
                     /locus_tag="VGR22_11565"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2129245.1"
                     /translation="MPQQQKPDPDGMLFDEIYATARGGDPRRIPWAHGVPHPLLLPWL
                     EQADRPPAGRSRALVVGSGLGDDAEALAARGWVVVGFDISEAAIAWARERFPDSRVTY
                     EVRSLFELPADWQGVFDLIVEIHTVQSLPVTRRQETVRAIANTMAPGGSLVVIAMTRD
                     VHVPLRGRPWPLTEAELRSFERHGLIETGRFTADDPTRDYPGRVRCHFAHPA"
     gene            12677..13120
                     /gene="mreD"
                     /locus_tag="VGR22_11570"
     CDS             12677..13120
                     /gene="mreD"
                     /locus_tag="VGR22_11570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEV2129246.1"
                     /translation="MARVIFGVVLFSMVFAQATIIPALNPLNVSPDFVLLLLFVVTLF
                     AGVREGLAWLFVAGILTDVLAMDPLGANGLALLPAVVLVEPARLPVFRANLLIPLVLV
                     LVATLLHGLIQGLIRGMVPDITLVLQALLHAILFPLFYLLLRWLE"
     gene            13166..15607
                     /locus_tag="VGR22_11575"
     CDS             13166..15607
                     /locus_tag="VGR22_11575"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEV2129247.1"
                     /translation="MARRKRIPYDPPHKRKKRRRRREVLRADQIFLNRRMLIAKSGVV
                     AAFGVLAAKLGVMQIQQGEEFRAEAEQNRIRAVKLPAPRGLILDRQGRRLAENRRSWE
                     VRVVEADLPEDGTPERQRVMDTLISALSLEDVLTIRPAAVPIGSQDTILQRVASMLGY
                     EGEDALDAITRWQGEIDKRVSYVLVTQLDIDGAARFRAASSELPGINVINELEYLIGS
                     VWAPRLPVTVKRDVPREIALQLKANLMYLPGVEIDDSALSRVYRGGEVMSHIIGYVQP
                     IDAASLYDPRNMTAGGNRIYDQQDVIGRSGIEATLEEHLRGQKGNRFEEVDVNGVRTR
                     IIPQTERAPVAGDNFTLTIDLEFQQAMGQALEKAIDQAANLKRELNTERRAKGLKEWK
                     IPNSGVAVAYDPRSGEIHGMVSYPYYDNQLFVSGLSGRKWTEYQDPARGKAFVNRAIN
                     ELYPPGSTFKVFLAASALSRGTLTTDTTYSCKGAIRVPFTYNLAQGNNYACWVAWQGG
                     TPHEVTDIYSAIAESCDIFFYNTAVEYTKPVDAFDPIFYYDYNLNQSAIVSDERHVFD
                     GLGIDPIAEDMQSKFWFGKPTGLELPESEGLFPDRQWKRETFAGEGWSVGDTINVSIG
                     QGAFTATPLQLALNVGALAMGGRYFRPHLITQRTSADGKVTEIGAEKIGELGIDAEHL
                     AVVREGMRRVCHTPEGTAYASRGKTKWPLTNPEGEEEILIGGKTGTAEFGEADEGYED
                     DALNTGARDTHALFTCYAPWDDPEIAVAVVIEAGGEGSTVAVPVADETLRAWFELTGR
                     RERGMVLSKEPMPVQ"
     gene            15637..16068
                     /gene="mce"
                     /locus_tag="VGR22_11580"
     CDS             15637..16068
                     /gene="mce"
                     /locus_tag="VGR22_11580"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEV2129248.1"
                     /translation="MAQDTMLLALGGLRLHHIGVVVEDLDVAAERYAALGFIGGERFS
                     VPEQAIEAIVYAAGEESYVELISPTDPEGPIARFMGKRGEGMHHVAYLVDDIEAMIAR
                     LHDAGIRLIDETPRKGTHGWTIAFVHPESTHGVLTELVQTD"
     gene            16394..17017
                     /locus_tag="VGR22_11585"
     CDS             16394..17017
                     /locus_tag="VGR22_11585"
                     /inference="COORDINATES: protein motif:HMM:NF024423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV2129249.1"
                     /translation="MPDLHARLAFFLATDALKEIRRANWVYSDARFETVAEHNWHTLL
                     LAMLFADVAPDGIDRNRVRDLLIVHDLVEVYAGDTPIWDDTARTTESEREREAGERLM
                     AMLPDDIFPSFDPFWREYQAQETAEARFARAIDSLHPIIMAWTEGAHGHANRDITPSR
                     LIERKRATLEAYPELWALVLRILQDAVDRGMLPPDEMMQCSEDADHA"
     gene            17010..18584
                     /locus_tag="VGR22_11590"
     CDS             17010..18584
                     /locus_tag="VGR22_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEV2129250.1"
                     /translation="MPDDRAITTDSDITIEPVYTAREEPVDLALPGDYPYTRGIHPGM
                     YRSRRWTMRQYAGFSSAEESNKRYRLLMSRGTTGLSVAFDLPTQLGLDSDDPRAYGEV
                     GRVGVAISTLDDMRRLFDGIDQEAVTTSMTINAPASLLLLMYQIVAEERGADAAKLDG
                     TIQNDILKEYAARGTYIFPPKPSMRLVTDTFAYCAEHLPKWNTISVSGYHIREAGSSA
                     AQEIAFTLANGVAYVEAAVNAGLHVDKFGPRMSFFFASHSNLFEEVAKFRAARRLWAA
                     IMRERFGATEPKAMMMRFHTQTGGVTLTAQQPLNNIVRVAYQALSAVLGGTQSLHTNG
                     YDEALAIPTEESATIALRTQQILSEETGVPDTVDPLAGSWFIESLTNQVEARVHEWMD
                     QVEEQGGAVTAIEHGFVQNAIAENAYRREMARSSGDEVVVGVNKYRHEDEELDIELHA
                     IDEEMVAAQQARVKQYKAEQNCEAVEAALARVRAAAEGTDNLLPVMKEALLAGATLGQ
                     TANALRDVFGEHQATL"
     gene            18634..19224
                     /locus_tag="VGR22_11595"
     CDS             18634..19224
                     /locus_tag="VGR22_11595"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEV2129251.1"
                     /translation="MDFLEVVRRRRMVRRFTDEPVPWETVERIVRVAQRAPSAGYSQG
                     VSFVAIDDAETRARVAEIVGEAGYVAAGFGPFVSSAPVQIVVCTSEEIYRARYREPDK
                     RRAGARANPWPVPYWHTDAGAALMLVLLAVVNEGLASAFVGVRDPDAVRAELGIPDDH
                     MPIGVVAIGHPAPDRRSSSLKRGRKPLESMLHRNRW"
     gene            19346..>20025
                     /locus_tag="VGR22_11600"
     CDS             19346..>20025
                     /locus_tag="VGR22_11600"
                     /inference="COORDINATES: protein motif:HMM:NF014042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HEV2129252.1"
                     /translation="MGLNIRYRKIVALAGGVGGAKLLQGLDGAAPEGVVTAIVNTADD
                     FELYGLHISPDLDTVMYTLAGIANPAIGWGIEADTRAALDAIARYGEDPWFIVGDQDF
                     ATHILRTHALHEGRTLTDVTLRLASGLGVSMPILPMTDDCVATEVRTADGWLAFQDYF
                     VARRHADEVLDVRFAGIDSARPSERVMMAVSEAELILFCPSNPLVSIEPILALSGVRD
                     AIMASPAPV"
BASE COUNT         3346 a         6475 c         6643 g         3561 t
ORIGIN      
        1 gggcatcaca atgctggcta tcactgccaa cagcgaaccc gccgattgtg ggcgggttcg
       61 ccggtcgaat acacgccaag ggagggcaac gtcgatacgc agtgaggcga tgggcgctgc
      121 cactccgtac gggcggactc catcttcgcc ctgtctgtat cgagcgaccc ctactcccag
      181 cgaacatcct cgtcgaggag ggtcgtcgcc ccctggtcgg cctgccgcac cagcttcgcg
      241 tacttggcca gcacgccgtt cttgtagcgc agctccggcg ccttccagtc cttcagccgc
      301 gctgcgatct cgtcgtcgga gagctccacc gccagcacgc ggttctcggc gtcgatgaca
      361 atctggtcgc cctcctggag cgcggcgatc gggcccccga cctgcgcctc cggcgcgacg
      421 tggccaatca tcaacccctt ggtagcgccg ctgaagcggc cgtccgtgat cagcgcgaca
      481 tcgtcaccgt ggccctcgcc cacgatggcg gcagtcacgg cgagcatttc ctgcatgccc
      541 gggccaccct tcggtccctc gtagcggatc actacgacat cgcccgagtt gatcttgccg
      601 ttgagcacag cctcgtgcgc ctcgtgctcc gagttgtaga cgcgggccgg ccccttgtga
      661 taggtgcgct cgtacccgaa cagcttcacc accgcgccgt ccggcgccag gttacctttc
      721 aggatgtgca ggccgccggt cttcttgatc gggtcggcga cggcatgcac cacgtcctgg
      781 ccctcggtcg ccgtggcggt ggccgcctcc tgagcaagcg tgcgccccgt cggcgtgatg
      841 gcagcaccgt cgatcagccc cgcttcagcc atccgctgcg cgatcaacgg aatcccgcca
      901 gccttgtcca gatcggcggc ggcgtacttg ccgcccggca tcatgtctgc gatcagcggc
      961 gtacgacgag agattcggtc aaaatcgtca atgtcgagat caatccccgc ctctcgggcc
     1021 agcgccagca ggtgcagcac ggagttggtg gagccaccgg tcgcgcagac gccagcgatc
     1081 gcgttctcga acgcggcgcg ggtgagcacg tcgcgcgggc ggacattgcg gcggagcaga
     1141 tccataatca acttgccccc cttctcaccg acttcctcct tgcgcgggtc gaccgctggc
     1201 ggcgatgccg agcccagcgg cgacatgccg aggaactcta tcgccatcgc catggtattg
     1261 gcggtgtact ggccgccgca ggcgcccgcg cccgggcagg cgtggttctc gagatccttc
     1321 aggtcctcgt cactcatctt gccggccgcg ttggcgccga tcgcctcgta gaccgagcgg
     1381 atggtcacct ccttgccgcg ccagaagcca ggcatgatgg tgccgccgta gaggcagaag
     1441 ctgggcacgt tgagccgcgt gagtgccatc gccaggcccg gcagcgtctt gtcacaggcg
     1501 ccgagggcga tgatgccgtc gaacatgtgc ccgcggccga ccagttccac cgaatcggcg
     1561 atgacctcgc gggagacgag cgaggtcttc atgccctccg tgcccatcgt taccccgtcg
     1621 gagatcgcga ccgtgttgaa ctccatcggc gtgccaccgg tagcccggac gcccttcttc
     1681 actgcctcgg cgagccgccg caggttgtag ttgcagggca tcgtctcgat ccatgtgttg
     1741 gcgatgccaa tgatgggttt cccgaggtcc tcgtcggtga agccgaccgc cttcatcatg
     1801 gcacgggcgc cggcgcggtc gttgccgtcg tagagggtgc ggctgttgaa ccgcatgtcc
     1861 acctcgccat tggtctcgct ggatttgccg ttatcgctca tttttcctcc aggcgtcgcc
     1921 tgtctgtgtt gggtggagca ggcacatgtg tgggcacgga gacatgcgcg tgcggcaacc
     1981 ccgcgtggca cacgactacc gactccgttg tcgtgtttcg ctggtcaagt ttcccctcat
     2041 tatcgccact tggcaaggat agccaggcgg gtgagactgg gaaaccgtgt agcgcattac
     2101 gcttcgcctt ccaaggagca caccatgcct ggtacgcccg accgattgct gttcggcatc
     2161 cacacgatcc agacgtggac ctggcccgag ttgcgggact gctggaaatg gttcgaatcg
     2221 ctcggcttcg acagcctctg gctccctgac cattacttcc cgacagccgg ccgtgacaag
     2281 ccgatgttcg aggcttggac gctgctctcc ggcttggcgc tggtcaccga gcgggcacgg
     2341 atcggcatcc tcgtgagcag caacaccttc cggcacccag cgctgctcgc caagcaggcc
     2401 gtcacggtcg atcacctctc cgagggccgg ctggagctgg gaatcggcgc ggggtggttt
     2461 gtcgaggagc atgaggtttt cgggcttgac tttccggaaa ccaggacgct ggtcgaccgc
     2521 ttcgccgagg cagtgcaact gatcgaccag tacctcacac atgaccagac ctcgttcgag
     2581 ggtgagcact accggctcaa cggcgcctgg aaccggccgg ccccggtgca gcagccgcgt
     2641 ccgccgctgg tgatgggtgc acacgggccg cgcatgctca aactggtcgc ccggtacgcc
     2701 gacacctgga attccttcgc gccgccggaa gagctggtcc gccgcaacca gcagttgacc
     2761 gactactgcg aagcaattgg gcgcgacccc cgcacggtga agcgctcgat gttctacggc
     2821 gtcaaccagt cagtggagga gaagccgtgg tcctccaccg acgctttcga ggactacatc
     2881 ggtcgctatc aggcggcagg gatgcaggag ttcatcctgc aaccgcccct tccggaccag
     2941 ttcggcgtgg ttgaacgggt ggcctcggat atactgccga gattgcgtga ccagttcacc
     3001 ccgctcgcag gaggagaggt atgactccat cgcccgatgg cgtgcgccag tttggcattc
     3061 agaccgtcca gacctggacg tggcccgaga tgcaccagcg ctgggcgtgg ttcgaggaga
     3121 tgggctggga gagcctctgg ttgccggacc atttcgtacc gacggcgaac ccgccaggcc
     3181 cgatgttcga agcgtggacg ctgctggcgg ccttggcgac ccacacgaag acggcccggc
     3241 tcggcgtgct ggtgagcagc aacacgttcc ggcacccggc gctgctcgcc aagcaggcgg
     3301 tgacgatcga ccatatctcc aatgggcgcc tggagctggg gatcggcgcc gggtggttcg
     3361 tcgaagagca cgagatgttc gggctggagt tcccggtggc ccggcagctc gtcgagcagt
     3421 acgccgaagc ggtcgatctg cttgatcggt acctcgggtc ggaccagatg accttcgacg
     3481 gcgagtacta ccacctcaag gatgcctgga accgcccggc accggtccag aagccgcgca
     3541 ttccgttgat gctgggcgcg cacggcctga agatgatcga tatcgccggg cagtacgccg
     3601 atagctggaa cagtcgtggt acgccacagg agatgtgcga gcgcaacgag cgcatggatg
     3661 aagcctgccg gcggcatgac cgcgatccga agagcatcaa gcgctcgatg ctctacgtcg
     3721 tcgcccagat gcccgacgag cgcccgtggg attccacgga tgccttcgag gactacgtcg
     3781 ggcgcttcag caaggctggc gtgcaggagt ttatcttcca gccacccccg gcggacaagc
     3841 tcgatatcgt cgagcgcgtt gctacggacg tcttgccaaa gctgcggtag gggtatgcgt
     3901 tggccggtat acttgcggga gcgcgcggag cgtgcactcg tacgtatact caaggaccat
     3961 acccatgggc gccgaactcg ccaacctgtt gctttccatc atccagattc tcagcctgct
     4021 gatcttcgcg cgggcgatct tctcctggtt cgacccgggg ttcagctcgc agatcggcag
     4081 ggtgctcttt cagattacag agccgatcat cgcgccggtt cggcaggtgc ttccgccgat
     4141 gggcatgctc gacctctcgg tgctggttac gctgattctg ctgatgatcc tgcagcagct
     4201 gatccgcagc gcgctcgtcg gctaggtccg gcagcgccga cgggcattgc ttgccaagga
     4261 tggcggcaat cccgtagagt cacggcagtc gtgcggcgaa tggccgcgat ttcccgcacc
     4321 agcgggtcaa cacaacaccg atgaggcctc acggcgggaa cgagcaggag agacgggaat
     4381 gatcatgcgc atgctgttgc tgctggtgat gatcgcgcag atggcgctca acaccgtcga
     4441 tccctccgac gaggaggagg cgcgcgagaa catgggcggt gagggcttcg ccaacatcgt
     4501 gatgctgctg gcgctgctcg gcgcgctgct gctggcggtg ctgatcgtgc tcgcgatcct
     4561 ctcgctgacc ggccagttcg agatggggct ggtggagcct gaggtgtagc gccagcggat
     4621 ctcacaatat cgcagcagga aaccgcccgg gagcatgtgc tcccgggcgg ttctgtagct
     4681 ggcttcagcg ttcacccctg caagcgacca ggcgtgtcgt ttcgaccgaa gcggcgaagc
     4741 cgcggagtgg agaaatttct tacccgccga gacctgatgt ccatgacgca agaagtttct
     4801 caacttcgca ccctctgagg tgctccggtt gaaacgacag gggtatggaa ccgaaagagc
     4861 gcctgctaat acgtcgctcg gccaccggag atatcgaaga ccgcaccggt cgagaacgat
     4921 accctgtccg agcagaggaa cgagacgagg tttgcaacct cctccggctg gccgacccgt
     4981 cccatcggaa ttcggctcgt catgtagtcg atgtgctcct gcgagatctg ctccaggatc
     5041 ggcgtggcaa tcaccgccgg tgtcactgcg ttgatcaaca cccccttggt cgcgacctcc
     5101 ttcgccagag acttcgtcag tccgatcact cctgccttgg cggcggagta gggggaggcg
     5161 tttggattgc cctccttgcc ggcgatcgac gcgatattca cgatccgtcc gtaaccctcc
     5221 tccatcatcg tcggcagcac tgtgcggcag ccgtagaaga cgccgttgag gtcgatgtcg
     5281 atcaccttgt gccattcgtc cagcgagagt tcccacgccg gcgccgaccg tcccgcgatg
     5341 ccggcattgt tgaccagcgc cgtgaccgtg ccgagggcgg ctcgcacggt ctccactgcc
     5401 gttgcccaac tctcggaatc ggtcacatcg agctgcacac ccatggtgcg gtcgccgatg
     5461 gctgcggcgg tctcacgagc ggcatcgagg tcgatgtcag cgacagcgac ggagaagccg
     5521 tcggcggcga gctggcgggc ggtcgccgca ccgatgccgc gtgccgcgcc ggtcacgatt
     5581 gcgacacgcc gcacggggac gtcaggggtg gccatgggtg agactccttg attgctgcgg
     5641 tcatcgatcg aacacactga tcctatccgc atagacggaa atgtaaaacc gggccctgat
     5701 tggagcccgg ctgcacgtcg gctcgcctgt ggttgaatgt cggtggctac tccggtgcgg
     5761 tgaaggctgc gcaggcattc tggccgccga agccgaagcc gttggcaact gctgccctga
     5821 cctcgcgctc acgcttggtg ttcggtgtgt aatcgagatc gcactccggg tcgggcgtat
     5881 ccaggttgat cgtcggcggg atcaccccat cccggatcgc caggatgcag gtcagcgccg
     5941 acaccgcccc cgccgcgccg agcaggtgcc cgaccatcga cttgttggcc gagatggaga
     6001 ggttgtaggc gtggccgcca tagacattct tgagcatgtt ggtctccgcg atgtcgccga
     6061 tggtggtggc agtcgcgtgg gcggcgacgt agtcgatgtc cgttggctcg agctccgctc
     6121 gccgcagcgc accccgcatc gcggtggctg cgccgcgacc ggacggatcc ggcgcggtga
     6181 tgtggtaggc atccgatgtg tagtagccac cgtgagcgaa ggcgatcggt gtggcgccac
     6241 gccgcgcggc atgctcctcc gactcgagga ttgctgccgc cgcgccctca gagaagacga
     6301 agccgtcccg atcagcgtcg aaggggcggc tggcccgttc cggagcctcg ttgcagcgcg
     6361 agagcgcgcg catattgctg aggctggcga tcgcgaccgg aatgatggcg gcctcggtgc
     6421 ctccggtgat gaccacctca acgtcgccgc gctcgatcat ggcgacggcg tcgacgaatg
     6481 cctgtacacc ggcggcacag gccgcaaccg acgtgaccgt tggcccgtga atgtcgaaca
     6541 tgatcgcgac ctggcaactg gccatgttcg gcgcgaagag cgggatgacc agcgagccga
     6601 cacgtttcgg gcctcgctct tccatgacgc gcacttcgct ggcgatcgtc gggatgccgc
     6661 cgccaccggt gttcatcatg atgccgatac gctcgcggtt gtcgtcggtg atctcgagac
     6721 aggcgtgctc gatcgcttcg cgggcggctg cgacgccaaa ctgcgagaag cggtccatcc
     6781 gccgcgcctt cttgatgtcc atatggtcgg caggattgaa gtcgggcact tcgccggcga
     6841 tctggacatc cagaccggag gcatcgaagc tctgaatctt gcggatgccg gatcgtcccg
     6901 ccgtcagtcc ttcccagaag gtgtccacgc cgatcccgag cggcgtaacc gcccccaacc
     6961 ctgttacgac cgctcgcgtc acgtcgaagt tcctttcccg tcgttgcctt agtgcgtagt
     7021 gaggtgcgag tgccgccgat gccgctcgca ggattccgcc tagtgtagcg ggaattgcaa
     7081 tgggtgttct acaggcaggc tcggaatcta ctgcaggtcc ctgagtccaa gggttggcac
     7141 gttccggtcg atgcggcggt tgagcccggc cagcatgctg ccggcaccga gctcgacgaa
     7201 tgtctccgtg cccatctcga tcatccggcg aaccgaatcc gtccagttga ccgggctttc
     7261 catgtgtgtg ccgagtgctc cgcggatggc gtcgccagtc gccagcggct cggtcgtgct
     7321 gttgtcgatg accgggacct ccgggtctcg gaaggcgacg ccggcgagct gcccgctgag
     7381 ctgctcggcc gccgcggaca tcagcggcga gtgcgaggcg atgctgacgc cgagacgggt
     7441 gaccctgcgc gcgccgcgct cctgggcgag agcggtggcg cgctccaagg ccgggatctc
     7501 gccggaaatc accgtctgac ccgggcagtt ggcgttggcg acgcggatta cgccctcgga
     7561 gctggcgtca cgcacgacac cttcgagctc ggcgtccgcc atgccgagga tggccgccat
     7621 gccgcccggc tgcttctcgc cggcctcctt catcaggcgg ccgcgctcgc gcacgatgcg
     7681 caaggcatcg gcgaagtcga tcgcgtcggc ggccactagt gcggtgaact gtcccagtga
     7741 atgaccggcc gcgacgatcg ggttgagagc cacgccgccg ccagctgccc gctggcgcag
     7801 caccgcaagc gacgcggtac tggtcgtgag cagagccggc tgggcgttgt acgtatcctc
     7861 aagctcgtcg gccgggccct caaagcagag ggtcgagagg gagaagccga ggatgtcgtc
     7921 ggcggtttgg aagatccggt gcgcctcgga ggactgctcg accaggtccc tgcccatgcc
     7981 gaccgtctga ctgccctggc ccgggaagat ataggcgatg ggcgtgttgc cgatgtcccg
     8041 tagtgccatg atagctccct ccaatgaatc ttttgaatct ttcatgctga gcgggcaagg
     8101 cgctgcggcg ccgccgtgtc gaagcatctc ttgcgcagcg ggcgtcaggt ttcggacaaa
     8161 gatgccgatg tcgaaggatg acaaccgcac gtggtttccc gctccgtcgt ttgcgcgcta
     8221 tcgggtcaac tctagcgcag catgccgccc tcggagttga tcacctggcc ggtgagccac
     8281 gccgcctcgt ctgatgcgag ccacgcaacc acgcgtgcgg cgtcctccgg tgtgccgaag
     8341 cgcccggccg gcatcatcgg cagcagccac tcccggatct catcagtgat ccagccggtg
     8401 tcggtcacgc cggggttgac cgcgttgacg gtgatgctct tgcctatcac cgccggggcg
     8461 aggctctggg tgaacgtgat gatcgccgcc ttgctcatgg cgtaggccag ctcgccggac
     8521 atcggtccga gatgctgccc cgaactgaag ttgatgatcc ggccgccgga ttctccctgg
     8581 aaacgtcggc agaactcgac cgataacatc gccgttgccc gggcattgac ctgatagtgg
     8641 cgatcgagct cttcggcagt gagtgcctcc cagccgtcac gcgtcgagta ggcggcgttg
     8701 ttgacgagga tcgagagcgg gcccagcgtc tcctccacca cgtcgagcag cgcgggcagc
     8761 ttgtctgcag gcgtcaggtc ccacgggatg ttcgtcgcgt cggcgccgat ctcgcgcagc
     8821 tcggcttcca ggcggtcggg ctcggaaacg tcactgtcgt acatctcggc atcgtaggca
     8881 gtgaagctgg tgaaggccac cttcgcaccg cgcgcggcta gcgcccggca gatcgccgcg
     8941 ccgatgccga tggagcgggt cgccccagtg acgagcgcga cccgcccgac gaggttcttg
     9001 gtcatggtga ggtcgtcctt ggatggatca atggatcgcg cacatggtca tttcgagcgt
     9061 agcagcgaag ccgaggagtc gagaaatctc ttgcgtcaca gaagtcggtt cccgacgggc
     9121 aagagatttc tccactgcgc gcccttcggg cgcttcggtc gaaatgacca tgtgcgggca
     9181 cgcgtgcact atcgttggct accgctggcg gagcatccac cagattcggt cccagccaac
     9241 gatgtagacg atgcccgcgg ccgtgaggaa tgtctccgac gccgtccgcg acacaaagac
     9301 ccagagcgac cacgccgcga caccaaagat cgccagctcg acgagtagcc gcgcccagcc
     9361 gggcaccacg atgaccggtg tcgggttgcg ggcagggtcg tcgggaacgg tcagtgcccc
     9421 ccacagggcc atcgcggtga tcgccatcgc tgccgtgagc acaccacccg gcagtccacc
     9481 atccccgagc tgccagcccg ccaagccgat cgcccagaag gcaaccagct cgagcaggaa
     9541 tcggaacgcg agcatcagcg ccggttgcat cggcggtgcg ccgtcgtcgt gagtcggtac
     9601 cgggcaggcg atcggcgcct ggtcgtctgt cacggcgcct acgtccctgt ccagcgccgc
     9661 aggatgacga ctgcgttctg cccgccgaga ccgaatgagt gcttggagat ggcatcgacc
     9721 tcggccttcc gcgcctcgct gacgacgtcg agtccgattt ccgggtccgg attgcggtgg
     9781 ttgcgggtcg gcgggatgca gttgtccgtc agcgtgcgga ccgccgacat gaattccagc
     9841 gcaccggcgg ctgccatgca gtggccgatt gcccccttga tagaggtgac cggcatcctc
     9901 ttcgcgcgtt cgccgaagac ctcgtggatc gccgcagctt cggccttgtc gccggctgtc
     9961 gtcgaggccg catgggcgtt gacatggtcg atctcgtctg gctccatacc ggcatccttg
    10021 agtgcggcgc gcatcgcccg ggccgcaccg aatccatgcg gctccggtgc gatcgggtgg
    10081 taggcatcgt tggaggtcgc gtatccggcg atctcggcga ggacgattgc actccgggcc
    10141 tgcgcgtgct ccaggtcctc cagcacgaac atcgccgcgc cctcgcccag cacgaacccg
    10201 gcgcgggtgg catcgaaggg gcggctcgcc ttctccgggt tgtcgttgga gtcggtgacg
    10261 agcgcgcgca tcgcctcgaa tccggccacg aagagtgggt ggagggtcga ctccgctgcg
    10321 ccggcgagca tcatcggcgc gtgcccgtcc cggattgcgc ggtagccgtc tccgacggcc
    10381 tgggtgccgg tcgcgcaggc cgtcaccacc gtcgagctcg gcccgccgag cccgaagcgg
    10441 tgctggatgg cgtaggccgc gagattgtgg ggaaacatca catgcaggtg cggtgggacg
    10501 cgagcgtagg agtcgcgctt gagcgccgtc tcgtacgccg gcaggaggtc gtcgtaggtg
    10561 ccgccgatgc acgtgcccag tgccgcgccc ccggtcgtga ggtccatgtt ggtctgcaga
    10621 tcgccgtcga gcagtccgcc ctgcagcatc gcctcgccgg cagcctccac cgcccatcgg
    10681 ctgtagcgtg aggtgttgcg gagcgccttg cgatcgagga agcggttctg gacggcattg
    10741 ttgtcaacgg taccgtgcag caggcaggtg tgtttgtcgg ggtcgagcaa ctccgagcgg
    10801 cggatacctg tctcgccgtc gcggatgcgc tgccagcttt cctcggcagt cggggcgagg
    10861 gaactgacgg caccgatgcc ggtgacgacg acccggcgtt gtggtgatgc agtgctcatc
    10921 ggtgtctgtc ctgtgtaccg cgccgggccg gtgcgaggtg gcgccggcga catgccagat
    10981 ctctggcggc gattgtgcca cgccgacacc cgcgcatggc gacggtggtc actgcgtcga
    11041 tgtgaccggg aggtcgtcgc ggagccccca ctcgccccat gacggcgcgt agttctgcac
    11101 gcggtcatga ccgagcatgc gcagcaccag ccaagtatgt gcagcgcggt agccgctcgc
    11161 gcagaaggtg atcgcgtcat cgtcaggcgc gaggcccata tcggtgtaga gggcccgcag
    11221 ctcgtcgatt ggcttgatcg tgccgtcgcg attgaggtgg tgaatccatt ccacgtgtcg
    11281 ggcgccgggg atggtgccct gcatccactc aaggtcggcg cgggtgtcga tgatggtggt
    11341 atcggtgtcg tcgttgctcg ccgagaggac atcgtcggcg gtggcaaaga gcgccgggtc
    11401 cagcgtcgcc gtgacgtcgc tctggctggc ggtcaccggc tcggtgctga tctcgttgcc
    11461 agccgccagc catgcccgga gcccaccgtc cagcatcgcg ccctcgccga tagagagtgc
    11521 atgcatcagc cagacaccgc gggcggcggt ggtaccactg atgtcctcgt agaacacgac
    11581 gtgccgctcg cggtcgatgc cgatttgacg gaaggcgtgt tcgatctccc ggacccagcc
    11641 ctcgatcttc tcggggtcgg agccaaggat cttgatcggg tagaggtcga gtgataccgc
    11701 accagggata tgcccctgcg cgtattggtc ctcgggacgg acgtcgacga tgagcaggtt
    11761 cgcatcgtcg tggtgctcgg caagccagtc gaccgagacg agcagatcgg ttatggcgtt
    11821 cgcagtcatg gtttctccaa agcgaggccg cgccggtcag gcagggtggg cgaagtggca
    11881 gcgcacgcga ccagggtagt ctcgggtggg atcgtcggcg gtgaatcggc ccgtctcgat
    11941 gagcccgtgg cgctcgaacg atcgcagctc ggcctccgtg agtggccacg ggcgaccgcg
    12001 cagcggcacg tggacatcgc gtgtcatggc aatgacaacc agtgacccgc ccggtgccat
    12061 ggtattggcg atggccctga cagtctcctg ccggcgtgtg accggcagcg actgcacggt
    12121 gtggatctcc acgatgagat cgaacacgcc ctgccagtcg gccggtagct cgaacaggct
    12181 gcgtacctcg taggtgaccc gactgtcggg aaagcgctcc cgcgcccacg cgatggcggc
    12241 ctccgagatg tcgaagccga ccaccaccca gccccgggcg gcgagcgcct cggcatcgtc
    12301 cccgagcccg gagccgacca ccagcgcccg gctgcgtcct gctggcgggc gatctgcctg
    12361 ctccagccac ggcagcaaca gcgggtgtgg caccccgtgg gcccagggga tgcggcgcgg
    12421 atcgcccccg cgcgcggtag cgtagatctc atcgaacagc atgccgtccg ggtcgggttt
    12481 ctgctgttgc ggcatggcgt ggcacctccg tccacatacg gacatcacca gaagccgaca
    12541 gataccatgg tacactcggc tacgttgcga tgcccccatc cgccggcgcc ggctgtcttc
    12601 cgaagagggg caattgtgcc cgattctgct gacaccacgg cgcatcggag ggcatcagga
    12661 gcacggtttc ccgcctatgg cgcgagtgat ctttggggtc gtcctgttct cgatggtatt
    12721 cgcgcaggca accatcatcc cggccctcaa tccgctcaac gtgtcgccgg atttcgtgct
    12781 gctgctcctg ttcgtcgtga cactgttcgc cggggttcgg gaggggctcg catggctctt
    12841 cgtggcgggg attctgaccg atgtgctggc gatggacccg ctcggggcga atggcctcgc
    12901 cctgttgccc gcggtcgtgc tggtcgagcc ggcccggttg ccggtctttc gggcgaacct
    12961 gctgatcccg ctggtgctgg tgctggtcgc gacgctgctg cacgggctca tccaagggct
    13021 cattcgcggg atggtgccgg atatcacact ggtgctgcaa gcactgctgc acgccatcct
    13081 gtttcctctg ttctacctcc tgttgcgctg gctcgaataa tgccggcctt gcataccgcg
    13141 tgaacacacg ttccgggaag tgctgatggc gagacgcaaa cgcatcccct acgatccgcc
    13201 ccataagcga aagaagcgtc gcaggagaag agaggtcctg cgcgctgacc agatcttcct
    13261 caaccgccgc atgctgattg ccaagagcgg cgtggtcgcg gcgttcggcg tgctcgcggc
    13321 caagctcggc gtcatgcaga tccagcaggg tgaggagttt cgtgccgagg ccgagcagaa
    13381 tcgcatccgc gcggtgaaac tgccggcccc gcgcgggctg atcctcgatc ggcagggcag
    13441 gaggctggcc gagaaccgtc ggtcgtggga ggtgcgcgtc gtcgaggcgg acctcccgga
    13501 ggatggcacc cccgagcgcc agcgcgtgat ggacaccctc atctcggcgc tcagtctcga
    13561 ggatgtactc actattcggc cggcagcggt gcccatcggc tcgcaggaca cgatcctcca
    13621 gcgcgtggcc agcatgcttg ggtacgaggg ggaggacgcg ctcgatgcta tcaccagatg
    13681 gcagggcgag atcgacaagc gggtgagcta tgtcctcgtc acccagctcg atatcgatgg
    13741 cgctgcccgc ttccgtgccg ccagctccga gctgcccggc atcaacgtca taaatgaact
    13801 cgaatacctc attggaagcg tctgggcacc ccggctgccg gtcacggtga agcgcgacgt
    13861 cccgcgggag atcgccttgc agctcaaggc gaatctgatg tacctgcccg gcgttgagat
    13921 cgacgacagt gcgctctccc gcgtctaccg agggggagag gtgatgtcac atatcatcgg
    13981 ctatgtgcag ccgatcgatg ccgcatcgct ctacgacccg cgcaacatga ccgccggcgg
    14041 caaccgaatc tacgaccagc aggacgtcat cgggcggtct ggcatcgagg cgacgctgga
    14101 ggagcatctg cgggggcaga agggcaaccg cttcgaggag gtggatgtca acggtgtccg
    14161 cacgcgcatt atcccccaga ccgagcgtgc accggttgcc ggggacaact tcacgctgac
    14221 gatcgacctc gagttccagc aggcgatggg ccaggcgctg gagaaggcga tcgatcaggc
    14281 tgcgaacctc aagcgggagc tcaacaccga acgtcgcgcc aagggcttga aggagtggaa
    14341 gatccccaac tccggcgttg cggtggccta cgacccgcgc tctggtgaga ttcacggcat
    14401 ggtgtcgtat ccctactacg acaaccaact ctttgtttcc ggtctatccg gtcgcaagtg
    14461 gacggagtat caggaccccg cccgcggcaa ggcgtttgtc aaccgcgcga tcaacgagct
    14521 ctacccgccg gggtcgacct tcaaagtttt cctggcggca tccgcgctct cccgcggtac
    14581 cctgacgact gacacgacct actcctgcaa gggtgccatc cgcgtgccgt ttacctacaa
    14641 cctcgcccag ggcaataact acgcctgctg ggtggcttgg caggggggta caccgcacga
    14701 ggtgaccgat atctacagtg ccatcgcgga gtcgtgcgac atcttcttct acaacacggc
    14761 agtggagtac acgaagccgg tcgacgcctt cgacccgatc ttctactacg actacaacct
    14821 caatcaaagt gcgatcgtca gtgacgagcg ccacgtcttc gatggactag ggatcgaccc
    14881 gatcgccgag gacatgcaga gcaagttctg gttcggcaag cccaccggtc ttgagctgcc
    14941 ggagtccgag gggctctttc cggatcggca gtggaagcgc gagacctttg ccggtgaggg
    15001 atggtcggtc ggcgacacca tcaacgtctc catcggccaa ggggccttca ccgccacccc
    15061 gctgcagctc gccctcaacg tcggcgcgct cgcgatgggc gggcgctact tccggccgca
    15121 cctcatcacg cagcggacca gcgccgacgg caaggtgacc gagatcggtg ccgagaagat
    15181 cggcgagctg ggcatcgatg ccgagcacct ggcggtggtc cgtgagggta tgcggcgggt
    15241 ctgccacacg ccggagggca cagcctacgc cagccggggc aagaccaagt ggcctctgac
    15301 caatcccgag ggtgaggagg agattctcat cggcgggaag acgggcacgg ctgagtttgg
    15361 cgaggccgac gaggggtacg aggacgacgc gctcaacacc ggtgcccggg acacgcatgc
    15421 attgttcacc tgctatgcac cgtgggacga cccagagatc gctgtggcgg tggtgatcga
    15481 ggcgggcggc gagggttcga cggtggccgt gcctgttgct gacgagacgc tgcgtgcctg
    15541 gttcgagctg accggccgcc gcgaacgcgg catggtgctg tcgaaggagc cgatgccggt
    15601 ccagtagggc acggacattg cgtgagggga ccggagatgg cgcaagacac aatgctgttg
    15661 gcactcggcg gcctgcgcct gcaccatatc ggagtcgtgg tcgaggacct cgacgttgcc
    15721 gccgagcgct acgccgcgct tgggttcatc ggtggcgagc gcttctccgt acccgagcag
    15781 gcgatcgagg cgatcgtgta cgcggcgggc gaggaaagct atgtcgagct gatctccccg
    15841 accgatcccg agggaccgat cgcgcggttc atgggcaagc gcggcgaggg gatgcaccac
    15901 gttgcctatc tggttgacga tatcgaggcg atgatcgctc gccttcacga cgccgggatc
    15961 cggctgattg acgagacacc gcggaagggc acgcatggct ggaccatcgc gttcgtccat
    16021 ccggagtcga cgcacggcgt cctcaccgag ctcgtgcaaa cagactgagc tgacacgcgc
    16081 agagctgagt cccatgtctg ccgcgttgac acccaacgtc cgccccccac tcggatgtgt
    16141 catttcgagc gaaaggcgag cgcatgtgag gctgcagtcg agacatctct tgccctcccg
    16201 agacaaactt ccgattcggg agagatgact ccactccgtg gcgaagccgc gggacctccc
    16261 ctgtgcgagg gccgtaggcc tgagtcgaat ggggtcgaga tgacaagcgg agatctccag
    16321 tttcctcttc aggttcggag tggcgtaggc ttgagtgtga aaagtccact gttcggatac
    16381 accgagaacc actatgcctg atctccacgc ccgcctcgcg ttcttcctcg ccaccgacgc
    16441 gctgaaggaa attcggcgcg ccaactgggt ctactccgac gcccgcttcg agaccgtcgc
    16501 cgagcacaac tggcacaccc tgctgctggc gatgctcttc gccgatgtcg ccccggacgg
    16561 catcgatcgc aaccgcgtcc gcgatctcct cattgtccat gatcttgtcg aggtctacgc
    16621 cggcgacacc ccgatatggg acgacaccgc ccgaaccacc gagtcggagc gcgagcgcga
    16681 ggcgggcgag cgcctgatgg cgatgctgcc ggatgacatc ttccccagct tcgacccgtt
    16741 ttggcgtgag taccaggcgc aggagaccgc cgaggcgcga ttcgcccggg cgatcgattc
    16801 gctccacccg atcatcatgg cgtggaccga gggtgcccat ggtcacgcca atcgcgacat
    16861 caccccctca cgcctgattg agcgaaaacg tgccaccctt gaggcgtatc cggagttgtg
    16921 ggctctggtc ctccggatcc tgcaggatgc cgtcgatcgt ggcatgctgc caccggacga
    16981 gatgatgcag tgtagcgagg acgccgacca tgcctgacga tcgcgccatc accaccgatt
    17041 ccgacatcac gatcgagccg gtctacactg cgcgtgagga gccggtggat ctcgctctac
    17101 ccggcgacta cccctacacg cgcggcatcc acccgggcat gtaccgctcg cggcgctgga
    17161 ccatgcgcca gtacgcgggc ttctcctcgg cagaggagag caacaagcgc taccgcctcc
    17221 tgatgtcgcg cggcacgacc ggcctgagtg tcgccttcga ccttcccacc cagctcgggc
    17281 tcgactccga cgatcctcga gcctacggtg aggtcggccg cgtcggcgtc gcgatctcga
    17341 cactcgacga catgcggcgc ctcttcgatg ggatcgacca ggaagcggtc accacgtcga
    17401 tgacgatcaa cgcaccggcc tcgctgctgc tgctcatgta ccagatcgtc gccgaggagc
    17461 ggggtgcaga cgcggcgaaa ctcgatggca ccatccagaa cgacatcctc aaggagtatg
    17521 cggcgcgggg gacctacatc ttcccgccga agccctccat gcggctggtg accgacacct
    17581 tcgcctactg cgccgagcac ctgccgaagt ggaacaccat ttcagtctcc ggctaccaca
    17641 tccgggaggc gggatcgtcc gccgcgcagg agatcgcctt cacactcgcg aatggtgtcg
    17701 cctacgttga ggcggcggtc aacgccgggc tccatgtcga caagttcggc ccgcgcatga
    17761 gcttcttctt cgcctcgcac tccaatcttt tcgaggaggt ggccaagttc cgggcggcgc
    17821 ggcggctctg ggcggcgatc atgagggagc gcttcggggc gacggagccg aaggcgatga
    17881 tgatgcgctt tcacacgcag accggtggcg tcacgctgac ggcacagcaa cccctcaaca
    17941 acatcgtccg cgtggcctac caggcgctct cggccgtgct gggcggtacg caaagtctcc
    18001 acaccaacgg ctacgacgag gcgctggcga tcccgaccga ggagtcggca accatcgccc
    18061 ttcgcaccca gcagatcctg tcggaggaga ccggcgtgcc ggataccgtc gacccgctgg
    18121 cagggtcgtg gttcatcgag tcactgacca atcaggtcga ggcgagggtg cacgagtgga
    18181 tggaccaggt cgaggagcag ggcggcgcgg tgaccgcgat cgagcacggg ttcgtgcaga
    18241 acgcgattgc cgagaatgcc taccggcgcg agatggcacg atcgtccggc gacgaggtgg
    18301 tggtcggcgt caataagtac cgccacgagg atgaggagct cgacatcgag ttgcacgcca
    18361 tcgacgagga gatggtcgca gcgcagcagg cgcgcgtgaa gcagtacaag gcggagcaga
    18421 actgcgaggc ggtcgaggcg gcgctggcga gggtgcgggc ggcggccgag ggcaccgaca
    18481 acctgctgcc ggtgatgaag gaggcgctgc tcgctggtgc gacgctcggc cagaccgcca
    18541 acgccctgcg cgatgtcttc ggcgagcacc aggcgacgct gtgacggatc gccctgtcaa
    18601 tgagccacga tcgatgtcgg cacgggggag cgagtggatt ttctggaggt ggtgcgacgg
    18661 cggcggatgg ttcgccgctt taccgacgag ccggtaccgt gggagacggt cgagcgcatc
    18721 gtccgggtgg cgcagcgcgc gccgagtgct gggtacagcc aaggcgtctc gttcgtggcc
    18781 atcgacgacg ccgagactcg ggcgcgcgtc gccgagatcg tcggcgaggc agggtacgtc
    18841 gcagccgggt tcgggccgtt cgtctccagc gcgcccgtcc agatcgtcgt ctgtaccagc
    18901 gaggagatct accgggcgcg gtatcgcgag ccggacaagc ggcgagccgg cgcccgcgcg
    18961 aatccctggc cggtcccgta ctggcacacg gatgccggag ccgcgttgat gctggtgctg
    19021 ctcgccgtgg tcaacgaggg gctcgccagc gccttcgtcg gcgtccgaga ccccgatgcc
    19081 gtccgcgccg agcttggtat ccccgacgat cacatgccga tcggggtcgt ggccatcggc
    19141 cacccggcgc cggacagacg ctcgtcatcg ctcaagcggg gcaggaaacc gctcgagtcg
    19201 atgctccatc gcaatcgctg gtaattcccg tcctgctgca gatggccgct gtaccgggct
    19261 cgtgtatgct cgtgtgagcg agcccgctct gattggcgcc ggatctgcga acacccgatc
    19321 tcgactctcg ccaaggacat cgaacgtggg cctgaacatc cgataccgga agatcgttgc
    19381 gctggcaggc ggggtgggtg gtgccaagct cctgcagggg ctcgatggtg cggcccctga
    19441 gggcgtcgtc accgcgatcg tcaacaccgc cgacgacttc gagctctacg gattgcatat
    19501 cagcccggat ctcgacaccg tcatgtacac cctcgccggg atcgccaacc ctgcgatcgg
    19561 ctggggcatc gaggccgaca cccgagcggc gctcgacgcg atcgcccggt acggcgagga
    19621 tccctggttt atcgtgggcg atcaggattt cgccacgcac atcctgcgca cccatgccct
    19681 gcacgagggc cgtacgctca cggatgtcac cctccgactc gcttctggtc tcggtgtcag
    19741 catgcccatc ctgccgatga ctgacgactg tgtggccacg gaggtgcgta ccgccgatgg
    19801 ttggctggcg tttcaggatt acttcgtggc gcggaggcac gctgacgagg tcctggatgt
    19861 gcgcttcgcc ggcatcgaca gcgccagacc atcggagcgg gtgatgatgg ctgtttctga
    19921 ggcggagttg attctcttct gtccgtccaa ccccctggtg agcatcgagc cgattctcgc
    19981 cctctccggc gtacgagacg cgatcatggc gtcaccggca ccggt
//
LOCUS       DASYWL010000327         3633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_73375, whole genome shotgun sequence.
ACCESSION   DASYWL010000327 DASYWL010000000
VERSION     DASYWL010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73375"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /gene="extP"
                     /locus_tag="VGR22_11605"
     CDS             <1..614
                     /gene="extP"
                     /locus_tag="VGR22_11605"
                     /inference="COORDINATES: protein motif:HMM:NF040969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="selenite/tellurite reduction operon b-type
                     cytochrome ExtP"
                     /protein_id="HEV2129253.1"
                     /translation="VFLHVHPVKIKRYATKVTYTFCLGGLSFFMFLVLTMTGVLLMFY
                     YIPSSDQAYQSMKDIEASVSFGQLMRNMHRWAAQGMVIAVFLHMGRVFYTGSYKPPRE
                     FNWVIGVLLLVTTFLLSFTGYLLPWDQLAYWAITVGSNIGGAAPIVGPQVNRILLGDF
                     QIGQSTLIRFYTLHVIFLPLVAAFLMAVHFWRIRKDGGISGPL"
     gene            661..1452
                     /locus_tag="VGR22_11610"
     CDS             661..1452
                     /locus_tag="VGR22_11610"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129254.1"
                     /translation="MAELANQPTGSAAQQIDEAKRRKLLELVRGRALARPTDLAEDEV
                     MVWPSLVVVEAVSAVVFLGILTALSILVNAPLLERANPNQTPNPSKAPWYFLNLQELL
                     LHMDAGLAGVIVPTVALVAIAAIPYIDRSPLGVGILGTSSKGRKIIAFTAVFTTVVLI
                     GMILLDEAAGTGTKFGIGTFLTEQFGAPEFVTGVLLPSVIMLATIAVFVAIIQGIYRP
                     TRRELIIALFTGFFVAYWVLAFIGTSFRGPGQELVWPWELPLEHH"
     gene            1530..2123
                     /locus_tag="VGR22_11615"
     CDS             1530..2123
                     /locus_tag="VGR22_11615"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEV2129255.1"
                     /translation="MTPLVRTLAGIAGGWLATKVFGLLETLQAAHGATPARKLTSRRS
                     FTRNATLGAIGIVTAQIAGGFVWLLWPNKTGAFGGEITVSAEAVPEVGGDPFRYSQGQ
                     FYVVNTDNGVLALWWKCPHLGCTVPFIAAENQFICPCHGSVYDYNGEYVAGPAPRGMD
                     IMPLEVQEDGAIAVNTDPSTIILGDVDRSEVAVPYPA"
     gene            2182..3177
                     /locus_tag="VGR22_11620"
     CDS             2182..3177
                     /locus_tag="VGR22_11620"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEV2129256.1"
                     /translation="MGAVQKLATVVIIGLVALATVLTVYIAREPDRRDEELTEQEQVA
                     ITRGTQLYITFCLQCHGPAGLGAVGNEENPRIGPPLNQIGAGVPKENLFYQSDDPVQQ
                     QLAEDLIRFRVTYGAPPDPRQTEKIMPSFRNDLSEEQIDELVTLIMEGDWDYVYNQAV
                     LQTGQQVAQAACHDEADEEGEAEECDHIEPGDIEAPPIYPTAPAPEVADPGETAAGEN
                     TGQDAPAEEGVAADADAETADVVLEAIDPYEWSETEPIVAPGSTITAVNAGSLEHNFT
                     VDELGIAEDLPTDGTNVTITIPEDAEPGQYEFYCSVPGHREGGMVGTLIVEESSS"
     gene            3188..3394
                     /locus_tag="VGR22_11625"
     CDS             3188..3394
                     /locus_tag="VGR22_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129257.1"
                     /translation="MLVSNLALVFGTLAETVELWLAIIMMISGIIITHLIQKWADSAG
                     EHPAAANVGLSGSAERREPKTMRQ"
BASE COUNT          631 a         1261 c         1129 g          612 t
ORIGIN      
        1 acgtgttcct gcacgttcat ccggtcaaga tcaagcgcta tgcgaccaag gtcacctaca
       61 ccttctgtct cggcggcctg tcgttcttca tgttcctcgt cctgacgatg accggcgtcc
      121 tgctgatgtt ctattacatc ccgtcgtccg accaggccta tcagtcgatg aaggacatcg
      181 aggcaagcgt ctcgttcggc cagctcatgc gcaacatgca ccgatgggcc gcgcagggca
      241 tggtcatcgc cgtcttcctc cacatggggc gggtcttcta caccgggtcg tacaagccgc
      301 cccgcgagtt caactgggtt atcggcgtgc tgttgctcgt gacgacgttc ctgctgtcct
      361 ttaccggcta cctgctgcca tgggatcagc tcgcctactg ggcaatcacg gtcggctcca
      421 acatcggcgg cgccgccccg atcgtcggac cgcaggtgaa ccggatcctg ctgggtgact
      481 tccagatcgg gcagagcacg ctgatccgct tctacaccct gcacgtgatc ttcctgccgc
      541 tggtcgcggc cttcctgatg gccgtgcact tctggcgcat tcgcaaggac ggcggcatct
      601 ccggcccgct gtaggcctcg agctcgcccc cgtacccacg tcgagagaag gaatccaggc
      661 atggcagaac tcgccaacca gccgaccggc tccgccgccc agcagatcga cgaggccaag
      721 cgccgcaagc tcctcgagct ggtgcgcggc cgcgcgctgg cccgaccgac tgacctggct
      781 gaggacgagg tcatggtctg gccgtcgctg gtggtcgtcg aggcggtctc cgcggtggtc
      841 tttctcggga tcctgaccgc gctctcgatc ctcgtcaacg cgccgttgct cgagcgcgcg
      901 aatcccaacc agacaccgaa cccgtcgaag gctccgtggt acttcctcaa cttgcaggaa
      961 ttgctgctgc acatggacgc cggtctcgcc ggcgtcatcg tgccgacggt cgcgctcgtc
     1021 gcgatcgccg ccatccccta catcgatcgt agcccgctcg gcgtcggcat ccttggcacc
     1081 tcgagcaagg gccggaagat aatcgccttc acggcggtct tcaccaccgt ggtgctaatc
     1141 ggcatgatcc tgctcgacga ggcggcgggc acgggcacga agttcggcat cggcaccttt
     1201 ctcaccgagc agtttggagc gccggagttc gtgaccggtg tcctgcttcc gagcgtgatc
     1261 atgctcgcca ccatcgccgt cttcgtggcc atcatccagg ggatctaccg gccgacccga
     1321 cgcgagctca tcatcgcgct cttcaccgga ttcttcgtgg cgtactgggt gctcgccttc
     1381 atcgggacgt cattccgggg tcccgggcag gagctcgtct ggccgtggga gctcccgctc
     1441 gagcaccact aggccgggtg gtaccgatac atgcaccgat cagccgggcg gacagatgcc
     1501 acccggctca cgagaaggaa cactcaccca tgactccact ggtacgcacg ctcgcgggca
     1561 tcgccggtgg gtggctggcc accaaggtgt ttggcttgct ggagaccctc caggccgccc
     1621 acggcgcgac cccggcacgg aaactgacat cccgccgttc gttcacgcgc aacgcgacgc
     1681 tcggcgcgat cggcatcgtg accgcccaga tcgcgggcgg cttcgtctgg ctgctgtggc
     1741 cgaacaagac cggcgccttc ggcggcgaga tcaccgtcag cgccgaggcc gttcccgaag
     1801 tcggcggtga cccgttccgc tactctcagg gccagttcta cgtggtcaat accgacaacg
     1861 gggttctcgc gctctggtgg aagtgcccgc atctcggctg caccgtcccc ttcatcgccg
     1921 ctgagaacca gttcatctgc ccgtgccacg gctcggtgta cgactacaac ggcgagtatg
     1981 ttgctggccc cgcaccacgc ggaatggata tcatgccgct cgaggttcag gaggacggcg
     2041 ccattgcggt caataccgac ccgtcgacga tcatcctcgg ggatgtcgac cggtccgagg
     2101 tggctgtgcc gtaccctgct tgatcgcacg taacgcgggt cgctgcgggc ggtcacgcga
     2161 gaggatgagg agaagccgaa cgtgggagct gtccaaaagc tcgcaaccgt ggtcatcatc
     2221 gggctcgtcg ccctcgccac ggtcctgacc gtctacatcg cccgcgagcc ggaccggcgc
     2281 gacgaggagc tgaccgagca ggagcaggtc gcgatcacgc gcggcacgca gctctacatc
     2341 accttctgcc tccagtgcca cggcccggcc ggactcggcg ccgttggcaa cgaggagaac
     2401 ccgcgcatcg gcccgccgct caatcagatc ggcgctggag tccctaagga aaatttgttc
     2461 taccagtcgg acgatccggt ccagcagcag cttgccgaag atctcatccg cttccgcgta
     2521 acctacggag cgcccccaga cccgcggcag accgagaaga tcatgccctc gttccgcaac
     2581 gacctcagcg aggagcagat cgacgagctc gtcacgctga tcatggaggg cgactgggac
     2641 tacgtttaca accaggcggt cctccagacc ggccagcagg tggctcaggc ggcctgccat
     2701 gacgaggccg acgaggaggg cgaggcagag gagtgcgacc acatcgagcc gggggatatc
     2761 gaggcaccgc ccatctatcc caccgcccct gcacccgagg ttgccgaccc gggtgagacc
     2821 gccgcaggcg agaacaccgg ccaggacgcc ccggccgagg agggcgtggc ggccgacgcc
     2881 gacgcggaga cggctgacgt cgtcctcgag gcgatcgacc cctacgagtg gagcgagacc
     2941 gagcccatcg tcgcgccggg cagcacgatc acggccgtca acgccggctc cctggagcac
     3001 aacttcacgg tcgacgagct cggcatcgcc gaggatctgc cgaccgacgg caccaatgtc
     3061 acgatcacca tcccggagga tgccgagccc ggtcagtacg agttctactg ctcggtcccc
     3121 ggccaccgtg agggtggcat ggtcggcacg ctgatcgtcg aagaatcatc atcctaggag
     3181 aggcacgatg ctggtatcca acctcgcgct cgtatttggc acgctcgcgg agacggtgga
     3241 gctctggctc gccatcatca tgatgatcag cgggatcatc atcacccacc tcatccagaa
     3301 atgggccgac agcgccggcg agcacccggc cgccgccaac gttggcctca gcggcagcgc
     3361 cgagcggcga gaaccgaaga ccatgcgcca gtagcaggcg cctgccttcg accaccgtga
     3421 cagcccgggt gcaaggggac gcacccgggc cgtttccgtt gatggcacgg gactacacag
     3481 catgagccag cccgacccca gcacctacct ctggtgtgac acctgccggc gcagctaccg
     3541 gcaccacgag acgcgcgacg gcgcctgccc cgtctgtggc ggcgagaccc ggccgatcgg
     3601 caagtaccag gcgatcgttc gcgggctgat gtc
//
LOCUS       DASYWL010000328         2514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_317026, whole genome shotgun sequence.
ACCESSION   DASYWL010000328 DASYWL010000000
VERSION     DASYWL010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317026"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VGR22_11630"
     CDS             complement(<1..500)
                     /locus_tag="VGR22_11630"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEV2129258.1"
                     /translation="MGQIRYKHEEQVLGKAYDSRIVRRLLGFLEPYRQQAWLTLGLVI
                     LVTGAELALPWLFSRAIDEVAPSGEQRLWMLNVIGAAFLLVVVIRFVGQWFQYRTAQW
                     MGHRVVYDMRNRMFRHLQALSMGYIDRRGVGTVMTRIQNDVSVIQEMFSGTALGLMSN
                     VLVLVG"
     gene            complement(509..2338)
                     /locus_tag="VGR22_11635"
     CDS             complement(509..2338)
                     /locus_tag="VGR22_11635"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2129259.1"
                     /translation="MNTALRILSYLKPYWRRMIVVYIALLIAVAGQTAIPLVLGEAID
                     RGAANGDTTFLWQAALFLIGLAALQGGFTFVRSFHLNVLAERVANDLRNQLYAKFQVL
                     PFQFYDQAQTGQLMSRATEDILQIRGMLLMGMRAVPHGLALLVIITVLLLREHVLLAL
                     VALSTTPFLLFWSFRFGVTIRPMFLKVQQQFGVMTSTLQENVAGGRVVRAFAQEAPES
                     NRFEDELDSLFHRNVRASNHWSFHYPLTLAMNGLSIAGVVWVGGYLALTGQVSIGTLV
                     AFERYTALLNEPVRWLGFVVNRVARALAAGERVFEILDRKPAITDSPNAQPMDDMRGE
                     VRFEHVSFAYTGGHGDALDDVSFEAHPGQITALVGPTGSGKSSVVNLIPRFYDPAEGR
                     VLIDDVDVRTITLASLRHNIGVVMQESFLFSMTVRENIAYGQPDATEEDVIAAAKAAQ
                     AHDFIMLMENGYDSLVGERGVTLSGGQKQRLAIARALLLNPRILILDDATASVDSETE
                     HEIQTALRTLMAGRTSFVIAQRLTTVRDADQILVFEGGRITQRGTHDELLAQEGFYRE
                     LYELQMKDQEEAARPGAVIEDEHPSATSAGAVTAGAPGGAEGA"
BASE COUNT          446 a          836 c          824 g          408 t
ORIGIN      
        1 atgccgacga gcaccagcac gttgctcatc aggccgaggg cagtgccgga gaacatctcc
       61 tggatcacgc tgacgtcgtt ctggatgcgc gtcatcaccg tcccgacacc gcgtcggtcg
      121 atgtacccca tgctcagtgc ctgcaggtgc cggaacatgc ggttgcgcat gtcgtagacg
      181 acacgatgtc ccatccattg agcggtgcgg tactggaacc actggccgac gaagcggatc
      241 acgaccacca gaaggaacgc cgcaccgatc acgttgagca tccacaggcg ctgctcgccg
      301 ctcggggcga cctcgtcgat ggcccggctg aacagccacg ggagcgccag ctcggcgccc
      361 gtgaccagga tcaccagccc gagtgtcagc cacgcctgct ggcgataggg ctcgaggaac
      421 ccgaggagtc gccgcacgat gcggctgtcg taggccttgc cgaggacctg ctcttcgtgt
      481 ttgtagcgaa tctggcccat gctgcctcct acgctccttc cgccccgccg ggagctccgg
      541 cggtgacggc ccctgcgctg gtagcgctcg ggtgctcgtc ctcgatgacc gctccgggcc
      601 gggccgcctc ttcctgatcc ttcatctgca gctcgtagag ctcccggtag aagccctcct
      661 gcgccagcag ctcgtcgtgc gtgccgcgct gggtgatgcg gccgccctcg aagacgagga
      721 tctggtcggc gtcgcggacg gtggtgagcc gctgggcgat cacgaacgac gttcgccccg
      781 ccatcagggt ccgcaacgcc gtctggatct catgctcggt ctcgctgtcg acgctcgcgg
      841 tggcatcgtc gaggatgagg atgcgcgggt tgagcagcag cgcgcgggcg atcgccaacc
      901 gctgcttctg accgccggag agcgtgacac cacgctctcc gacgagcgag tcgtacccgt
      961 tctccatcag catgatgaaa tcgtgcgcct gcgccgcctt cgcggcggcg atcacgtcct
     1021 cctcggtcgc gtccggttgg ccataggcga tgttctccct gaccgtcatc gagaagagaa
     1081 agctctcctg catcaccacg ccgatgttgt gccgcagcga cgcgagcgtg atggtccgca
     1141 cgtcgacatc gtcgatcagc accctcccct cagcggggtc atagaagcgc gggatgaggt
     1201 tgaccactga gctcttcccg gaaccggtgg gcccgacgag cgccgtgatc tgccccgggt
     1261 gcgcctcgaa ggagacatcg tccagggcat cgccgtggcc gcccgtgtag gcgaaggaga
     1321 catgctcgaa gcgcacttcg ccgcgcatgt cgtccatcgg ctgcgcattc gggctgtcgg
     1381 tgatcgccgg cttgcggtcg aggatctcga agacccgctc gcccgctgcc agcgcgcggg
     1441 ccacacggtt gacaacgaag ccgagccagc gcaccggctc gttgagcagc gcggtgtagc
     1501 gctcgaaggc gaccagcgtg ccgatggaca cctgcccggt gagcgcgagg tagccgccga
     1561 cccagaccac cccggcgatg ctgagtccgt tcatggccag cgtcagcgga tagtgaaacg
     1621 accagtggtt gctcgcgcgg acgttgcggt gaaagaggct gtccagctcg tcctcgaacc
     1681 ggttggactc cggtgcctcc tgcgcgaagg cacgcaccac ccggccgccg gcgacgttct
     1741 cctgcagcgt cgacgtcatc accccgaact gctgctgcac cttgaggaac atcggccgga
     1801 tggtgacgcc gaaccggaac gaccagaaga gcaggaatgg cgtggtcgag agcgccacca
     1861 gggcgagcag gacgtgctcg cgcaggagca gcacggtgat gatgaccagc agcgcgagac
     1921 cgtgcggcac cgcccgcatg cccatcagca gcatgccgcg gatctgcagg atgtcttcgg
     1981 ttgcgcggga catcaactgg ccggtctgcg cctggtcata gaactggaac ggcagtacct
     2041 gaaacttcgc gtagagctga ttgcgcaggt cgttggcgac ccgctccgcc agcacattga
     2101 gatggaaaga gcgcacgaag gtgaacccgc cctgcagcgc ggccagcccg atcaggaaga
     2161 gggccgcctg ccagaggaag gtcgtatcgc cgttggcggc accacggtcg atcgcctcgc
     2221 ccagcaccag cgggatcgca gtctgcccag caacggcgat gagcagcgcg atgtagacca
     2281 cgatcatgcg gcgccagtac ggtttcaggt aggacaggat ccggagagcg gtgttcacga
     2341 agggcctctt ggttcgttat ctaggcatcg cgacctgacg ggcacacgac ggcgcgtccg
     2401 catgccggcc cgtcagtcta ccgcccatcc cttggtatgt aaacggcagg ctgacaatgg
     2461 cagcacgaga accatgccag cgggcagctc agcaacttac tgctgcgaca ggag
//
LOCUS       DASYWL010000329         2693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_599204, whole genome shotgun sequence.
ACCESSION   DASYWL010000329 DASYWL010000000
VERSION     DASYWL010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599204"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1937)
                     /locus_tag="VGR22_11640"
     CDS             complement(<1..1937)
                     /locus_tag="VGR22_11640"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV2129260.1"
                     /translation="MDDIFGIPMTGLVLTLVALFALCLLSVAWVGWRHPVIFKLGVRN
                     IPRRRAQSTLIVVGLMLSTLIMAAALGIGDTINYSMSADVYDNLGHIDELVVASADSE
                     ARVDLTADGTFDAATFPRIEAMLEGDPNVDGLMPMLDARASVTHETMQLAEPGIIITG
                     IDASRLEGFGGLQDTDGRLIDFGAVGADGVVLSASLAEKLDATAGDRLTLHVNNASVV
                     RTVTAITEDSYLVGTRRSLSSSLVTPGLAMPLASLQTLIGQDGKLSAVAISNAGGVRG
                     GIDYSQAVTTTLREELAGQGLGVAPIKERRVAEAKSFATVFTSVFLVLGLFSIAAGIL
                     LIVLIFTMLAAERRAEMGMERAVGTHRRQLIQQFIAEGSGYALVAGLVGTGLGVLAAL
                     GIGQGMKLIFGDYVPIEPYVAPRSLVAAYCLGMVITFMAVVGSSWKISRLNIVAAVRD
                     IPDISSPQRKKWTLVWAGLLLLAGGLMTFAGAGGTALLFTTGMSLWPFGIILALRYFG
                     VAARPVFTAVGLYLLVFWLLPEDAFNAIFGNYDSGFSQFFVAGVFLVIATTVITINNL
                     DVLLAGLSRLGGPFRSQLPAVRIAIAYPGATKGRTGLTVAMFSLIIFSLVMMATMNQT
                     SIELALGDESNAGWDVRADART"
     gene            complement(2000..>2693)
                     /locus_tag="VGR22_11645"
     CDS             complement(2000..>2693)
                     /locus_tag="VGR22_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2129261.1"
                     /translation="TKTYATGSATVHALKGVDFAVKRGEMVAVMGPSGCGKMTLLNTL
                     SGLDRVDKGTIWLEGQNLAQLSDRKRTDYRARRMGFIFQVYNLLPVLSAVENVELPLL
                     VSGVRPKQARDRAMSALETVALAKWAKHRPAELSGGQRQRVTIARALVNDPAIVWADE
                     PTGALDSTTADEIMHLMRELNERHQLTFVLVTHDPGIGDRCDRIVRMRDGEIVDSVRH
                     DPLVEVAAVATG"
BASE COUNT          494 a          851 c          896 g          452 t
ORIGIN      
        1 gttcgagcat cggcgcggac atcccagccg gcgttcgact cgtctccaag cgccaactcg
       61 atcgacgtct ggttcatcgt cgccatcatc accagcgaga agatgatcag gctgaacatc
      121 gcgacggtca gacccgtccg ccctttggtg gcgcccgggt acgcgatcgc gatgcggacg
      181 gcgggcaatt gagagcggaa cgggccgcca agccggctga gaccggcgag gagaacgtca
      241 aggttattga tggtgatgac cgtggtggcg atgaccaaaa agacccccgc gacgaagaac
      301 tgtgagaagc cgctgtcgta gttaccaaag atcgcgttga aggcatcttc cgggaggagc
      361 cagaagacga gcaggtacag accgactgcg gtgaagaccg gccgggcggc gacgccgaag
      421 tagcgtagcg ccagtatgat accgaatggc cagaggctca tgcccgtggt gaacaggagc
      481 gcggttccac cggctccggc aaacgtcatc agcccgccag cgagcaggag gagccccgcc
      541 cagaccagcg tccacttctt gcgctgggga gaggagatgt cggggatgtc ccggaccgct
      601 gcgacgatgt tgaggcggct gatcttccag gaggagccga ccaccgccat gaacgtgatc
      661 accatgccca agcaatacgc tgcaacgagg ctgcgtgggg cgacgtatgg ctcaatcggt
      721 acatagtcgc cgaagatcag cttcatcccc tggccgatgc ccagtgcggc gaggacgccg
      781 agcccggtgc cgaccagccc ggcgacgagc gcgtagccgc tgccctcggc gatgaactgc
      841 tgaatgagtt gccgccgatg ggtgccgacg gcgcgctcca tccccatctc cgcgcgccgc
      901 tcggcggcga gcatcgtgaa gatgagcacg atcaggagga tgccagccgc gatcgagaag
      961 agcccaagca ccaggaagac ggatgtgaac accgtcgcaa acgacttcgc ctcggcgacg
     1021 cgtcgctcct taatcggggc gacgccgagc ccttgacccg cgagctcctc gcggagcgtg
     1081 gtggtcacgg cttgagagta gtcgatgccg ccgcggacgc ctcccgcgtt cgagatggcg
     1141 acggccgaga gcttgccgtc ttgcccgatc agggtttgca ggctcgcgag tggcatggcc
     1201 agaccggggg tcacgagact actgctgagg ctccgtcgcg tgcccaccaa gtagctatcc
     1261 tccgtgatgg cggtgaccgt gcgcacgacc gaggcattgt tgacgtggag ggtgagccga
     1321 tcacccgctg tggcgtccag cttctcggcg aggctggcgc tcagtacaac tccatccgcg
     1381 ccgaccgccc cgaagtcgat cagccggcca tcggtgtcct gcaaaccacc gaatccctcc
     1441 aatcgtgacg cgtcgatccc cgtgatgatg attcccggct cagcgagctg catggtttcg
     1501 tgggttaccg atgctcgggc gtcaagcatc ggcatcaggc cgtcgacgtt cggatcaccc
     1561 tcgagcatag cttcgatgcg cgggaaggtc gcggcgtcga acgtgccgtc agcggtgagg
     1621 tcaacccgcg cttcgctgtc ggcgctggcg acgacgagtt cgtcgatgtg gccaaggttg
     1681 tcgtagacgt cggcgctcat cgagtagttg atggtatcgc cgatgccgag ggcggcggcc
     1741 atgatcaggg tcgagagcat cagcccaacg acgatgaggg ttgactgcgc ccggcggcgg
     1801 ggaatgtttc gtaccccgag cttaaagatg actggatgcc gccagcccac ccacgccacc
     1861 gacagcaggc agagcgcaaa cagggccacc aaggtcagca cgaggccggt catggggatc
     1921 ccgaagatgt catccatggt ctgattcctc cgttgcgtgc gatgatcgga ttccagtgcg
     1981 agcgctcggg gaccgggcct taacccgtcg cgactgccgc aacctctact agtgggtcgt
     2041 gtctgacact gtcgacaatc tccccgtcgc gcatgcggac gatgcggtcg cagcggtcgc
     2101 cgatgcccgg gtcatgcgtc acgagcacga aggtcagttg gtgacgctcg ttcagctccc
     2161 gcatcaagtg catgatctcg tcggccgtcg tggagtcgag cgcgccggtg ggctcgtcgg
     2221 cccagacgat tgccgggtca ttgacgagcg cacgggcaat ggtcacccgt tggcgctgtc
     2281 ctcccgagag ctcggctggc cgatgcttcg cccacttcgc cagcgcgacg gtttccagcg
     2341 cactcatcgc ccgatcgcgt gcctgcttgg ggcgcacgcc ggagacgagc agcggtagct
     2401 cgacattttc gaccgcactg aggaccggca gcaggttgta gacctggaag atgaagccca
     2461 tccggcgggc gcggtagtcc gtccgcttgc ggtcggacag ctgggcgaga ttctgcccct
     2521 ccagccagat cgtgcccttg tcgacccggt cgaggccgga cagcgtgttt agcaacgtca
     2581 tcttgccgca gccggacggg cccatcaccg cgaccatctc gccgcgcttg acggcgaagt
     2641 cgaccccctt gagggcatgg acggtggctg atcccgtcgc gtaggtcttg gtc
//
LOCUS       DASYWL010000330         1981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_280281, whole genome shotgun sequence.
ACCESSION   DASYWL010000330 DASYWL010000000
VERSION     DASYWL010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280281"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VGR22_11650"
     CDS             complement(<1..414)
                     /locus_tag="VGR22_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV2129262.1"
                     /translation="MAQHAAESTIVTPSPKRRGLSEETQAGFIAISPWILGFIVFTAF
                     PFVASFYFSFTRYDVINPPEWIGLANYERLLTRDRYFPKAVQNTLIYALLYIPSNIVT
                     SLGAAMLLNSARRMKGFFRTAFYLPAMTPAVATAYI"
     gene            complement(544..1938)
                     /locus_tag="VGR22_11655"
     CDS             complement(544..1938)
                     /locus_tag="VGR22_11655"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV2129263.1"
                     /translation="MTYRPSLSRRRFIGTSTIAGASLAGSLNIGIARSAFAQGTPQAL
                     GDASGSLTEWGFGIQETNPLARARILAFQAAYPDVDLEIVESYDEAKLLTAAASDTLP
                     DLLWLGRFETAPWASRGVLQPLDEFVERDGYDLSMFYDWALEEASYDGELYGIPGGAD
                     VRALFVNQDYLDEVGANARELDTSDWDRLSELGAQLVQKSGDVIDRWGFDHKLQAGYI
                     WLWGHGNGGNFINEDGTEVYFDDQEIVEALDWGVQAYDAQGGYTDYLTISSTWQGDEQ
                     FARGEVSMAMYEQWMLSAAVATIDPDMNFWILPIRERGSGPDGPMVTFSGGNAWYITS
                     GAQNPDAAWEFIKFLHTVDTWMIGARAVKTLREDTNAVYFPSLTGNIEADRRQIEELY
                     EPVNEAFDHAVELFPTLVEASTFRQIANSTVAGQLDQIMMDEGVIPALSGERSPQEAL
                     EEADAAAQEAIDFS"
BASE COUNT          339 a          597 c          668 g          377 t
ORIGIN      
        1 gatataggcc gtcgccacgg cgggggtcat ggcgggcagg tagaaggcgg tacggaagaa
       61 gcccttcatg cggcgagcgc tgttcagcag catcgccgcg ccgagcgagg tgacgatgtt
      121 cgacgggata tagagcagcg cgtagatgag cgtgttctgg acggctttcg ggaagtaccg
      181 gtctcgtgtc agcagccgct catagttggc caggccgatc cattctggcg ggttgatgac
      241 atcgtatcgg gtgaagctga agtagaagga ggctacgaag ggaaacgccg tgaagacgat
      301 gaagccgagg atccatggac tgatggcgat gaatcccgct tgcgtctcct cgctgagccc
      361 gcgacgcttc ggagatggcg tgacgatggt ggattctgcc gcgtgctgtg ccatgaagaa
      421 atctctccca cgcttaccgt cgagcgggag cggctccccg gctcgccgtc atcgtgaccc
      481 gcatggggaa ccggggagca cggtgcgtgg gcactcctcc acgcgccgtg ctccgattgt
      541 ggctcaggag aagtcgatcg cttcctgtgc ggcagcgtcc gcctcttcga gcgcttcctg
      601 tggcgaacgc tctcccgata gcgcagggat cacgccctcg tccatcatga tctggtcgag
      661 ctggccggcg acggtcgagt tcgcgatctg gcggaaggtg cttgcctcga ccagtgtcgg
      721 gaagagttcc accgcgtggt cgaatgcctc gttgaccggc tcgtagagtt cctcgatctg
      781 ccggcggtca gcctcgatat tgccggtgag cgacgggaag tagaccgcgt tcgtgtcctc
      841 ccgcagggtc ttgactgccc gcgcgccgat catccatgtg tcgacggtgt ggaggaactt
      901 gatgaactcc caggccgcgt ctggattctg cgcaccggag gtgatgtacc acgcattgcc
      961 gccggagaac gtcaccatcg ggccgtccgg gccgctgccc cgctcgcgga tcggcaggat
     1021 ccagaagttc atgtcgggat caatggtggc gacggcggcg ctgagcatcc actgctcgta
     1081 catcgccatc gagacttcgc ctcgggcaaa ctgctcgtcg ccctgccatg tgctggagat
     1141 ggtcaggtag tccgtgtagc cgccttgggc gtcgtaggcc tgcacgcccc agtcgagcgc
     1201 ctcgacgatc tcctggtcat cgaagtagac ctcggtaccg tcctcattga tgaagtttcc
     1261 gccgttgccg tggccccaga gccagatgta gccggcctgc agcttgtggt cgaagcccca
     1321 acggtcgatg acgtcgccgc tcttttgcac cagctgtgcg ccgagttcgc tcagcctgtc
     1381 ccaatcggaa gtgtcgagtt ctcgggcgtt cgcgccgacc tcgtcgaggt agtcctgatt
     1441 gacgaagagt gcacggacat cagcaccgcc ggggatgccg tagagctcgc cgtcgtagct
     1501 cgcctcctcc agtgcccagt cgtagaacat cgagaggtca tagccgtcgc gctcgacgaa
     1561 ctcgtcgagc ggttgcagca cgccgcggga cgcccacggg gctgtttcga agcgaccgag
     1621 ccagagcagg tcgggcagcg tgtcggaggc cgcagcagtg agcaacttcg cctcgtcgta
     1681 ggactcgacg atctcgaggt cgacgtccgg gtaggccgcc tggaaggcga ggatgcgcgc
     1741 acgagccaag gggttggtct cctggatgcc gaagccccac tccgtcagcg aaccggaagc
     1801 gtcgcccagc gcctgcgggg tgccttgggc aaaggcgctt cgggcgatgc cgatgttgag
     1861 cgatccggcc aggctggccc cggcgatggt gctggtgcca atgaaacgtc ggcgtgacag
     1921 tgacggtcgg tacgtcatga tattacgctc cttattgata taccacctcg gcgcgaggag
     1981 c
//
LOCUS       DASYWL010000331         2888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_92349, whole genome shotgun sequence.
ACCESSION   DASYWL010000331 DASYWL010000000
VERSION     DASYWL010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92349"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VGR22_11660"
     CDS             complement(<1..226)
                     /locus_tag="VGR22_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HEV2129264.1"
                     /translation="MATTRAPGSTAETQLAIEGMTCAACVRRVEKALEKVEGVSNASV
                     NLATERARVAYDPARVDLAALTAAVQKAGYT"
     gene            414..806
                     /locus_tag="VGR22_11665"
     CDS             414..806
                     /locus_tag="VGR22_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129265.1"
                     /translation="MVNQPERPDAASRHGSVQSDGNRARRCPWCGSDDVRLVQRGFTG
                     PTDERDQYISCNRCDRVTYEIISRSARDMRLGQYRTGGTYRDSRRQTRYDITRVLKAG
                     SNEYLLYVKPIIRSENSTPSGPSRRGRF"
     gene            867..2480
                     /locus_tag="VGR22_11670"
     CDS             867..2480
                     /locus_tag="VGR22_11670"
                     /inference="COORDINATES: protein motif:HMM:NF001964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEV2129266.1"
                     /translation="MVQASPASHRALAEEVARRRTFAIISHPDAGKTTLTEKLLLYGG
                     AVQLAGSVTARKSQRHAASDWMEMEQERGISITSTVLAFPYQDYHVNLLDTPGHQDFS
                     EDTYRTLTAVDSAVMVLDGARGIEPQTLKLFEVTRRRRTPIFTFINKMDRPAQHPLEL
                     LEEIEKTLGLATYPLNWPIGDGGDFRGVYDRKSKMVHLFERTEHGAKQAPVRVSGVDD
                     PLLDEQLGEERAATLREELELLDLAGTEFDLDEVLAGNLTPVSFGSALTNFGVQLFLD
                     HFVEMAPAPQPQETSSGATIGPANEPFSGFVFKIQANMDKRHRDRVAYMRIVSGRFER
                     DLDVWVPRLKRKVRLSRPMRLFGSDREVVEEAYAGDVVGLASTGTFTIGDTISSEDIG
                     TFPPLPRFQPEHFALLRHTRADRYKQFLKGLSQIEEEGAIQLFYPVSTGSREPVLAAV
                     GQLQFEVVRYRLENEYNVETELEPLTYTAARWVDGDDGEITTIGNGRGRMRAEDRDGR
                     PVLLFSTEWDLRYAEENAQNVTFSALAGV"
     gene            2619..>2888
                     /locus_tag="VGR22_11675"
     CDS             2619..>2888
                     /locus_tag="VGR22_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129267.1"
                     /translation="MAETIEKTRLPHWDMTTIYPGLDSPEFMNAVEEVRAKIADLEAA
                     VQKLEPGEEITDATVAIFALTNRINDVLQQVSTVRAFVAGYTSVDS"
BASE COUNT          517 a          938 c          931 g          502 t
ORIGIN      
        1 ccgtgtagcc ggccttctgc accgcagcgg tgagggcagc gaggtcgaca cgggcagggt
       61 cgtatgccac cctggcgcgc tcggtcgcca ggttgacgct ggcgttcgac accccctcga
      121 ccttctcgag ggccttttcg acccgccgca cacaggcggc gcacgtcatc ccctcgatcg
      181 cgagctgcgt ctcggcggtg ctgcccggtg ctcgtgttgt tgccatgctc gttgctcctc
      241 gcacgaaccg atggtgcgaa tgccattatg gcatcgatta taccccttcc gggtatatcg
      301 cgggagtggt cacggtcgtt gaagcgcggc atgggattgg tatagtttcc ctgacatgtc
      361 cgatcggggt gccgcgtgct ccgcgcagcg gccacgcgca gcagggaggg gccatggtca
      421 accaaccgga gagaccggac gccgcgtccc gacatggaag cgtccagagc gacgggaacc
      481 gcgcacgtcg ctgcccgtgg tgtggcagtg acgacgtgcg gttggtccag cgtggtttca
      541 cgggcccgac cgacgagcgc gaccagtaca tctcctgcaa ccggtgcgat cgggtaacct
      601 acgagatcat ctcgcgcagc gcccgcgaca tgcggctggg gcagtaccgc acgggtggga
      661 cctatcgcga cagtcggcgg cagacgcggt acgacatcac acgtgtgctc aaggccgggt
      721 cgaacgagta tttgctctac gtcaagccga ttatccgttc cgaaaacagt acccccagtg
      781 gtccctcacg gcgcggacgc ttctgacaac gccatctatc cgcctgtcac ttcccgtcat
      841 tcgcccttcg cttcattgag gatttcatgg ttcaggcatc acccgcgagc catcgtgcgc
      901 tcgccgagga agtcgcgcgc cgtcgaacat tcgcgatcat ctcccatccg gacgccggca
      961 agaccacact cacggagaag ctgctgctct acggcggagc agtccagttg gcaggctccg
     1021 tcactgcccg caagtcgcag cggcacgcgg cctccgactg gatggagatg gagcaggaac
     1081 gcggtatctc catcacctcg accgtattgg cgttcccgta ccaggactat cacgtcaacc
     1141 tgctcgatac ccccggtcac caggactttt cggaggacac ctaccgaacg cttaccgcag
     1201 tcgacagtgc cgtgatggtg ctggatgggg cacgcggcat cgagccgcag acgctcaagc
     1261 tcttcgaggt cacgcgccgc cggcgtacgc cgatttttac cttcatcaac aagatggatc
     1321 gccctgcgca gcacccactg gagctgctcg aggagatcga gaagacactc gggctcgcga
     1381 cctacccgct caactggccg atcggcgatg gtggcgactt ccgcggcgtc tacgatcgca
     1441 agtcgaagat ggtccacctc ttcgagcgca ctgagcacgg tgcgaagcag gcgccggtgc
     1501 gggtttcggg ggtcgacgat ccgctcctgg atgagcagct cggcgaggag cgcgccgcga
     1561 ccctgcgcga ggagttggag ctgctcgatc tcgccggcac cgagttcgat ctcgacgagg
     1621 tgctcgccgg caatctcacg ccggtcagct tcggatcggc gctgaccaac ttcggcgtcc
     1681 aactcttcct cgaccatttc gtcgagatgg ccccggcacc gcagccgcag gagacgtcga
     1741 gcggcgcgac gatcggcccc gccaacgagc cgttctccgg cttcgtcttc aagattcagg
     1801 cgaacatgga caagcgccac cgcgaccgcg tggcctacat gcggattgtc tccggtcgct
     1861 tcgagcgtga tctggatgtc tgggtgccac gcctcaagcg gaaggtgcgt ctctcccggc
     1921 cgatgcggct cttcggctcg gaccgtgagg tggtggagga ggcgtatgcc ggcgacgtcg
     1981 tcggcctggc ctcgaccgga accttcacga tcggcgatac catcagcagc gaggacatcg
     2041 ggaccttccc accgctgccg cgcttccagc cggagcactt cgcgctgctg cgccacaccc
     2101 gcgccgatcg ctacaagcag ttcctcaagg gtctctccca gatcgaggag gagggcgcga
     2161 tccagctctt ctatccggtc tcaacgggca gccgggagcc tgtgctggcg gccgtaggcc
     2221 agttgcagtt cgaggtcgtg cgctaccggc tcgagaacga gtacaacgtc gagacggagc
     2281 tcgaaccgct gacgtacact gcggctcgat gggtggatgg cgacgatggt gagattacca
     2341 cgatcggcaa tggccggggc cggatgcgtg ccgaggatcg cgacggtcgc ccggtactgt
     2401 tgttctcgac cgagtgggac ctgcggtacg ccgaggagaa cgcgcagaac gtgacgttct
     2461 ctgcccttgc cggggtgtag cgatagttgc agcgccaaac cgcgtgtcat gtcgaccgga
     2521 gcggcgctgc gagccgagcc cgagcgccgg ggggacacgg tgtccacccg tacgggacgg
     2581 ttccgtgtca agccggcgaa cacgaaaggc acaacgacgt ggcagagacg atcgagaaga
     2641 caaggctccc gcactgggac atgacgacga tctaccccgg actggactcg ccggagttca
     2701 tgaatgccgt cgaggaggtc agggcgaaga tcgctgacct ggaagcggcc gtgcagaaac
     2761 tcgagcccgg agaagagatc accgatgcta cggtggcgat cttcgcgctg acgaaccgca
     2821 tcaatgacgt gctccagcag gtcagcaccg ttcgcgcctt cgttgccggg tacacctcgg
     2881 tcgactcc
//
LOCUS       DASYWL010000332         1529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_673755, whole genome shotgun sequence.
ACCESSION   DASYWL010000332 DASYWL010000000
VERSION     DASYWL010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673755"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(361..726)
                     /locus_tag="VGR22_11680"
     CDS             complement(361..726)
                     /locus_tag="VGR22_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129268.1"
                     /translation="MEFVHLMHERLATTVMIYFLLVGLWGLYEFARGGVLAGSIAGAL
                     VIGQVLVVVQVLFGVALWVDGFRPGDGTHVLYGITAILVMPFVWTYMRDRAPRQALII
                     YSLIALFIFGLAIRGMTTA"
     gene            complement(838..>1529)
                     /locus_tag="VGR22_11685"
     CDS             complement(838..>1529)
                     /locus_tag="VGR22_11685"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2129269.1"
                     /translation="HEHGDHIRTLPKIAGDDLQLIATSGTARHAGLPAERTEIVNTRA
                     PVRVASATVHMLPVRHDASDPCGYHIEIGGVRLTVLTDLGSWQDHLVDAVASSNLVLL
                     EANYNETMLARGPYPARLKRRVASEIGHLGNIACGQAMVSAVRTGRVEATWWLSHLSQ
                     TNNSPQQAELDVRETLNRADLDAGVTALPRRDPGPVWAFEPTNRKLLMPTTIVTSSKC
                     AAAQLGLPGLG"
BASE COUNT          287 a          483 c          497 g          262 t
ORIGIN      
        1 gtccatgacc ctctggtagt ctcgggattc tgagtccacg acgactcctt tcagatcttg
       61 tctcggccac cccccggcga ccacgaaacg ccgcaacgac actgtgtgca ggtgccgtgc
      121 gatcaacatg tacgcatcag actgtacgat cagattgtac gttgcgagcg gcagagtgtc
      181 aatcatgccc gtacgccgta gccgagacta ccgatgtggt cgtatcggca cacctaacag
      241 ccttatagca accgcagccg gccggtctcc ggtcacgggc aactaccctg gatccgggcc
      301 ggttttcctg cctcactcgc gaaggagggt cgggtctaca ggagccgtct gtcggcgcgg
      361 ttacgcggtg gtcatccctc ggatggcgag gccgaagatg aagagcgcga tgaggctgta
      421 gatgatcaac gcctggcgag gtgcacggtc gcgcatgtag gtccacacga atggcatcac
      481 caggatcgcc gtgatgccgt agagtacatg tgtgccatcg cccggccgga acccgtctac
      541 ccacagcgcc acaccgaaca gcacctgcac gaccaccagc acctggccga tcaccagagc
      601 gccagcgatg ctgccagcaa gcaccccacc tcgcgcgaac tcgtagagtc cccacagtcc
      661 gacgaggagg aagtagatca tcaccgtggt ggcgaggcgc tcatgcatca ggtgaacgaa
      721 ttccatgcag ttctccgggc gctggcgtcg agtccggaac cgaggacgag gttcggcaga
      781 cggccctgag tataggggga ggggacagcg aggacagggg gacggttccg gattgcctca
      841 gccgagaccc gggaggccga gctgcgcggc agcgcacttc gaggaggtga cgatcgtggt
      901 gggcatgagc agcttgcggt tggtcggctc gaaggcccag acaggaccgg gatcacggcg
      961 gggcagggcg gttacgccag catcgagatc ggcccggttg agcgtctccc gaacgtccag
     1021 ctccgcttgc tgaggactgt tgttggtctg cgagagatgg ctgagccacc atgtcgcctc
     1081 cacccgaccg gtcctcactg cgctgaccat tgcctggccg caggcgatat tgcccagatg
     1141 cccgatctct gacgctaccc gccgcttcag acgagccgga tacggaccac gggcgagcat
     1201 cgtctcgttg tagttcgcct caagcaagac gaggttgctg ctggcgaccg catcgaccag
     1261 gtgatcctgc cacgagccga ggtcggtcag cacggtgagc cgaacgccgc cgatctcgat
     1321 atggtagccg cagggatcac tggcgtcgtg ccggaccgga agcatatgca cggttgcgct
     1381 ggcgacgcgc accggtgcac gagtattgac gatctccgtt cgctcggctg gcaggcccgc
     1441 gtgccgcgcc gtgccgctgg tcgcaatgag ctggaggtcg tcccccgcga tcttcggcag
     1501 cgtccggata tggtccccgt gctcatgcg
//
LOCUS       DASYWL010000333        14097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_430093, whole genome shotgun sequence.
ACCESSION   DASYWL010000333 DASYWL010000000
VERSION     DASYWL010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 14097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14097
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430093"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VGR22_11690"
     CDS             <1..366
                     /locus_tag="VGR22_11690"
                     /inference="COORDINATES: protein motif:HMM:NF012611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129270.1"
                     /translation="TPALSWALNYVDSYRREFLPANLIQGQRDFFGAHTYKRLDKEGI
                     FHTEWAPVAPAPVVESEPDTTEDRAEVEEAGERSARGGEVPASADRRALHPEADTELE
                     PDPEEEGERTPEATRKAKY"
     gene            468..2114
                     /gene="zwf"
                     /locus_tag="VGR22_11695"
     CDS             468..2114
                     /gene="zwf"
                     /locus_tag="VGR22_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEV2129271.1"
                     /translation="MTETKTRPTDYDYATDSTIADMDHVGHQFEVKEPTTSTNPLRGG
                     LRLERSPSPCVMIIFGVTGDLTARKLIPALYDLTVGYPLPEGFSIVGVARRDWDDETF
                     RQKMREAVENGARTPVTDESWKLFAQGLFYVSGNFSDEQTYTDLRARLEVIDRERRAH
                     GNRLFYLATSPTYYGPIIKHLGAAQIAARQDIYWDRTNNWNRIVVEKPFGKDVETARA
                     LNAEIGAVFSERQIYRIDHYLGKETVQNVLAFRFANILFEPVWNRQYVDHVQITVAES
                     IGVEGRGGYYEESGALRDMVQSHILQLLSVIAMEPPALFNGNALRDEKVKVLRAVVPP
                     RGEKVNELSVRGRYEAGYVGGRAVPAYRDEKEVSPQSRTETYVAMKLGIDNWRWAGVP
                     FYLRTGKRLPRRVTEIAIEFKQVPHLMFESIGNVELTPNVISMRIQPDEGIALKFAAK
                     VPGPATQLRPVRMDFLYGQSFGEAGPDAYERLLLDAMLGDPTLFARRDEVETAWSMMQ
                     PILDGWDERPVPVYPYEAGTWGPSKAETLVQRDGRVWRRP"
     gene            2221..3450
                     /locus_tag="VGR22_11700"
     CDS             2221..3450
                     /locus_tag="VGR22_11700"
                     /inference="COORDINATES: protein motif:HMM:NF042449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HEV2129272.1"
                     /translation="MAQTFGTKPVLASAWQADTLDTGSIHDELNRLWNELGGPRHGGD
                     APGEMVADPHYGGGGLMRANTLNLIAVATDMREVNLIQQTISTLHDFLPSRAIIIITY
                     DGYDHEAGVFGPRYDVRVELKEQVTDGEDNDNLRFEVITIGADRREIGHLASLVTPLL
                     TADLPDFLWWPSGDFAKHPLFDDLTPVVDRLIVDTAHLGHEIRAIAAIRGIIAEESDA
                     PIVGDFTWLRLEPWRQLIAQFFDPEDVQVCLKTIDQVTIRYAETRDDGSSGLAAALLM
                     TGWLGSRLGWEVIDPLERQKRGGLFAPLRASESALTRDIELRLIPDTSPHARFSLRSV
                     ELVSTGKASGTFAVERTDSDDIVTHSETPNNPPVSRMVYSQRPTSQDMIGQEMQRFGA
                     DPYYEEAMRFAVRLIPN"
     gene            3647..4405
                     /gene="pgl"
                     /locus_tag="VGR22_11705"
     CDS             3647..4405
                     /gene="pgl"
                     /locus_tag="VGR22_11705"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEV2129273.1"
                     /translation="MIDYGERGQVTVARKSSQLPLLATEELAVVYLRAANRPQPALIT
                     LSGGSTPKAMFGIMKDPRFWRRIEWSKVHLLWGDERWVPIEDEQSNAGEALRGFIEEM
                     KLSEEHYHPWPTHLTDPNAAAEAYEMTIRILSGTMEGTPVLDLVFLGMGDDGHTASIF
                     PGTDAVHEQDRLAVAHHVPKLDATRLTLTPPILNAAREAVFLVSGTGKAEMLHKVLDG
                     PIDVDAMPSQAIRPTAGRLRWFVDEAAAAQLERE"
     gene            4411..5103
                     /gene="rpiA"
                     /locus_tag="VGR22_11710"
     CDS             4411..5103
                     /gene="rpiA"
                     /locus_tag="VGR22_11710"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001924.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEV2129274.1"
                     /translation="MDTNTRLERIGAQAAGEVQPGMIVGLGTGSTASAFVRALGARVA
                     EGLSITGVPTSTETERLARSLNIPLTELNRVDVIDLCIDGADEIDPALNVVKGRGGAL
                     LFEKLVARRTDRYIIIAADDKLVNRLGTRLPLPVEIVPMGWQHTARELERIGLGPVLR
                     TGTDGNSFVTDSGHYIVDCETGGIEDAPALASKIKAITGVVDHGLLIGLVDLAMTIDA
                     TGRITTHESAIA"
     gene            5084..5794
                     /locus_tag="VGR22_11715"
     CDS             5084..5794
                     /locus_tag="VGR22_11715"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV2129275.1"
                     /translation="MNQRLPETLADLPSPSTSAGILLVLAGIASVLGFFARGEQDIWG
                     DEAVLESIQAIDAPGLELAVRVSNFAFDTAGALLVAIVLVAVALLLRRPVFVLEMAVV
                     IAFRVAVIVLKPLFASPRPGTEHHVDLAHVPETFGYPSGHAFTAPVVTGMFVLFIDSL
                     NLPSVFKGIAIVLATLVTALGMFSRIWIGAHWPSNTIGGVLYGIAAVALMRIIASFIV
                     LQFGSLFSTAARGRSLRR"
     gene            complement(5730..6260)
                     /locus_tag="VGR22_11720"
     CDS             complement(5730..6260)
                     /locus_tag="VGR22_11720"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEV2129276.1"
                     /translation="MKRTTHLMMGAAAGLLVAAPSPLPVEIGAVWLGVAGGGFPDWLD
                     LRSELRGPLRLRHRGISHSLLFALFATALVGLPLRAVADAVDLPGGMALSPETALVWT
                     LAFAAGLLTHLAGDACTHAGIRPLLPVSRWKMWLLPKLLRSRSDGYLNAVAMLVSVGA
                     IGLSLLWRIGNQIGAL"
     gene            complement(6273..8198)
                     /locus_tag="VGR22_11725"
     CDS             complement(6273..8198)
                     /locus_tag="VGR22_11725"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV2129277.1"
                     /translation="MTVINRFGAFDPETFKFFAADQGGAATMDRETGDDSTNAAEGAD
                     NDEQRVTFASLGLSDDLLQSITAVGFETPTPIQERTIPSLLAGNDVIAQAQTGSGKTA
                     AFGLPIIENIDQKRREVQALVLCPTRELAMQVAQALEEFGRHKRVETLAIYGGQPYER
                     QLRGLQRGVHIVVGTPGRVMDHMRRGTLKLDALDFFVLDEADEMLDMGFVDDIEWVLQ
                     HAPEERQTALFSATMPPRIAELATKYMNEPHRISVRGKEMTVAEIEQTYYEVPQPRKV
                     DALTRILDVEVPSSAMIFCRTKHMVDQLGEALQSRGYAVETIHGDLSQAQRDRVMRRF
                     RTGQAEILIATDVAARGLDIPDVSHVINFDIPESPETYVHRIGRTARAGKGGNAITLV
                     TRREVRWIRTIERVTKAKIQYRPLPSAADVNKRRREMLKKLVSDAADDREDYESYLEP
                     VRELTDGHDAVELAAAILKLYAEETGRAATDEIEQDDLSHASSRPSGERGAGGGRARG
                     AESGMTRLFINIGHNFRVRPQDFVGAIANEAKIPGRAIGAIDIHDAFSFVDVPENMAE
                     QVIDAMNAASVKGRPVNVEVASDGAGGRDDRRGPRRSGPGSPPRGGRFGGPRRDEEGR
                     GRRSSGPGRSGPRRDRW"
     gene            complement(8787..9011)
                     /locus_tag="VGR22_11730"
     CDS             complement(8787..9011)
                     /locus_tag="VGR22_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129278.1"
                     /translation="MPARDELTQAQRDQLERIAAVYGITPERLVALTTERQERDRNAA
                     RAEVARPAVVRKRPAVPFHIGESAGGFRRP"
     gene            complement(9172..9849)
                     /locus_tag="VGR22_11735"
     CDS             complement(9172..9849)
                     /locus_tag="VGR22_11735"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEV2129279.1"
                     /translation="MTDTSLATWSPRSLPSVEPLHGHRVIVRPTDVTGDADALFEATC
                     DGGPALWDYLPYGPFADEAAFEQWLARHVADPTMLTETVVDGRSGQPVGSASYMHMEP
                     EFGVIEIGHIFFGVSLQRTPAATEAIYLMLRHVFDDLGYRRLEWKCGAASARSVRAER
                     FGFTYEGTFRQHRIVKGRNRGTAWFSIIDKEWPAVKASFEALLSPDNFDTNGQQIRRL
                     SVVRSGA"
     gene            complement(9846..10352)
                     /locus_tag="VGR22_11740"
     CDS             complement(9846..10352)
                     /locus_tag="VGR22_11740"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEV2129280.1"
                     /translation="MSRLVRPVTAYRLPVRVRFHECDPLGHVNNAVYLNWLEQAAIDH
                     AALVGWSQENLEREVGGVFVARRHEIDYLRPAYEGDHLEVVTWPDSMQMATALRRYVI
                     RRAIAPNGTPELIEGPDVAALESGEVLVRAVTRWALIRREAARPLRIPKRVIDDFLET
                     GDDEDQQP"
     gene            complement(10349..12106)
                     /locus_tag="VGR22_11745"
     CDS             complement(10349..12106)
                     /locus_tag="VGR22_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129281.1"
                     /translation="MTSHRQMDEWHDHDDMRVERRPGKREVDLYVRTYNTLLESSGAV
                     SVSSLEPAHLTASSSLHAGAEEREPDLSAFLYSVNRIPACLPQVKHIILGQSHRAFSR
                     GGYPEIESWQAVSAPARRRRWYFNGTDTLGAYISSASDLDDLIPSIVAYEIEWNKMHD
                     LLNESPDVRDRLLGKGEPIAADDPAVRKALYLSEPDFERLQRVWGGDLMPNLKRMATD
                     HLRIDIQMLGGTYLGYGRSVRAWWRPVYDVLAARGLLGRPTYFVSSNTHSIANVFSGT
                     AIRRERQLVEFIRETEHPELLPELEKREQGKMRGAWENVLYFAARSWFYGPHRAEERR
                     QRQREEEQVGITYLEPDGSVDVGVQVFELAKLDPSLFDPRLGELDPRGSDAVIININY
                     PLGMTAFHIMTQVGAAIENTRGVYILGKAATLNGRIGDIMMSNVVFDEHSGNTYWFEN
                     AFTYDSVAPYLTFGSALDSQKAVTVKGTYLQNEGYLDFYYRGNYTVVEMEAGPYLSAL
                     YEDVFLERHPTDEAINLAPLSRHIDLGIIHYASDTPYTRAHTLGARGMSFYGMDSTYA
                     STVAILQRIFDLEQERMEE"
     gene            complement(12193..12828)
                     /locus_tag="VGR22_11750"
     CDS             complement(12193..12828)
                     /locus_tag="VGR22_11750"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEV2129282.1"
                     /translation="MRLKTGQKIVMVGDSITDAGRRDIDPPYGAGYVSLVRSIATALY
                     PELDLTWENRGIGGDTVRNLADRWVSDVLDERPDVLTVMIGINDVWRRFGDNPLEAVP
                     ADEYQSTLVTLLRATRERGAPDLYVGSPYMIEADTSDLMRSAMDLYGLLAREVAEEVG
                     AVFIDVQAAFDRVLEHSESSDWADDKVHPNGPGHAVIALEFLRAFGVEILG"
     gene            13359..>14097
                     /locus_tag="VGR22_11755"
     CDS             13359..>14097
                     /locus_tag="VGR22_11755"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEV2129283.1"
                     /translation="MPITIAIGGISHETHAFAGQLTTLADFDGRALLTGNRLLEHARG
                     SDSVLGGIIAAARPDVELVPTLFASAMPGGPVVHEAWDDLSRRLLTRLRTAAIRPPAI
                     DGVVLALHGAMATTESRDPDSALVLAVREIVGDGVPIVVVLDAHGTPSDRLIETADAV
                     LAYRTYPHIDMQATGAAALAVCRSLVNGDVQPRTAVRRLPVLLPLTAQRTNGPTPMTR
                     PVRQAVALERLPAVLQVNLLPGFPFNDV"
BASE COUNT         2415 a         4809 c         4482 g         2391 t
ORIGIN      
        1 accccggctc tgagctgggc gctcaactac gtcgatagct accgccggga gttcctgccc
       61 gcaaacctga tccagggcca gcgggacttc ttcggcgccc acacctacaa gcggctggac
      121 aaagagggga tcttccatac cgaatgggcg cccgtggcgc cggcgccggt ggtggaatcg
      181 gagccggata ccaccgagga tcgcgctgaa gtcgaggaag ccggcgagcg ctccgcgcgc
      241 ggcggcgagg tccctgcaag cgccgacagg agagcgctcc acccggaggc tgacaccgaa
      301 ctggagccag atccggagga agagggcgag cgcacacccg aagcgacccg caaggccaag
      361 tactgatcac acacctggca cgacggcagt gccgtcgtgc caggtcgaaa aggcattccg
      421 cactggattc gcgccatgat ctgtagagcg catgccaggg gacgtccatg acagagacca
      481 agacgcggcc caccgattac gactacgcca ccgacagcac aatagccgac atggaccacg
      541 tcgggcacca gttcgaggtc aaggagccga ctaccagcac caacccgctc cgtggcgggc
      601 tacgcctcga acggtcaccc tcgccctgcg ttatgatcat cttcggcgtg accggtgacc
      661 tcaccgcccg caagctgatc cccgcactgt acgacctcac cgtcggatac cccttgccgg
      721 aaggcttctc catcgtcggc gttgcccggc gcgactggga cgacgagacc ttccgccaga
      781 agatgcgcga ggcagtcgaa aatggcgccc gcacaccggt caccgacgag tcctggaagc
      841 tcttcgcgca ggggctcttc tacgtttccg gcaacttcag cgatgagcag acgtacaccg
      901 acctgcgcgc gcggctggag gtaatcgatc gcgagcgccg cgcccacggc aaccgtctct
      961 tctacctcgc gacctcgccc acctactatg gaccgatcat caagcatctc ggcgccgcac
     1021 agatcgccgc ccggcaggat atctattggg accggaccaa caactggaac cgaatcgtcg
     1081 tcgagaaacc gttcggcaag gatgtcgaga cggcccgggc gctcaacgcg gagatcggtg
     1141 ccgtcttctc ggagcgccag atctaccgca tcgaccacta cctagggaag gagacggtcc
     1201 agaacgtcct cgccttccgc ttcgcaaaca tcctcttcga gccggtatgg aatcggcagt
     1261 acgtcgacca tgtccagatc accgtcgccg agtccatcgg cgtcgagggc cgcggtggct
     1321 actacgagga gtccggcgcg ctgcgggaca tggtgcagag ccatatcctg caactgctca
     1381 gcgtcatcgc gatggagccg ccggcgctct tcaacggcaa cgcgctgcgc gacgagaaag
     1441 tgaaggtgct tcgcgcagta gtgccgccga ggggcgagaa ggtcaacgaa ctgtcggtcc
     1501 gcggacgcta cgaggccggc tacgtgggcg gccgtgctgt ccctgcctac cgggacgaga
     1561 aggaggtcag cccgcagtcg cgcacggaga cctacgtcgc gatgaagctc ggcatcgaca
     1621 actggcgctg ggcaggggtg cccttctacc tgcgcaccgg caaacgtcta ccgcggcgcg
     1681 tgaccgagat cgcgatcgag ttcaagcagg tgccgcacct gatgttcgag tcgatcggca
     1741 acgtcgagct cacacctaac gtgatcagca tgcgcatcca gccggacgag ggcatcgcgc
     1801 tcaagttcgc agccaaggtg ccgggcccgg ccacccagct ccgccccgtg cgaatggact
     1861 tcctctacgg gcagtccttc ggcgaggccg gcccggacgc ctacgagcgc ctgctcctcg
     1921 acgcgatgct cggcgacccg acgctgttcg cccgccgtga cgaggtcgag acggcgtggt
     1981 cgatgatgca gcccatactc gacggctggg acgagcgccc ggtgccggtc tacccgtacg
     2041 aagctggcac gtggggcccg agcaaggcgg aaacgctcgt gcagcgcgac gggcgcgtct
     2101 ggcgccgccc gtagaacaca cgctgcgggc cagttccgga gcgtggacct tctcacaggc
     2161 accgtcccgc acgaccgtcg actcgcatgg ccgtcggcac aacggcgttg gagatacacc
     2221 atggcacaga cattcggcac caagccggtt ctcgcctcgg cgtggcaggc ggataccctc
     2281 gacaccggct ccatccatga cgagctcaac cggctctgga acgagctggg cggacccagg
     2341 cacggcggtg acgctcccgg tgagatggtc gctgatcccc actacggtgg gggcggcctc
     2401 atgcgcgcca acacgctcaa cctcatcgcc gtcgcaaccg acatgcgtga ggtcaacctg
     2461 atccagcaga cgatctcaac cctgcacgac ttcctgccct cgcgcgcgat catcatcatc
     2521 acctacgacg gatacgatca tgaggctggt gtgtttggac cgcgatacga tgtccgtgtg
     2581 gagctcaagg agcaggtgac cgatggcgaa gacaacgaca acctgcgctt cgaagtcatc
     2641 accatcggcg ctgaccggcg cgaaatcggg catctggcca gcttggtgac tcccctgctc
     2701 accgctgacc tgcccgactt cctctggtgg ccgagcgggg actttgccaa gcaccccctc
     2761 ttcgatgacc tgactccggt tgtcgaccgc ctgatcgtcg acaccgccca tctcgggcac
     2821 gaaatccggg cgatcgcggc aatccgaggc atcattgccg aggagtcgga cgccccgatc
     2881 gtcggcgact ttacgtggct gcggctggag ccgtggcggc agctgatcgc gcagttcttc
     2941 gatccagagg acgtacaggt gtgcctcaag acgatcgacc aggtgacgat ccgctatgcg
     3001 gaaacgcgcg acgacggctc ctccggtctg gccgccgcgc tgctgatgac cgggtggctt
     3061 ggcagccggc tcggctggga ggtgatcgac ccgctggagc gccagaagcg tggcgggctc
     3121 ttcgcgccgc tgcgcgcctc cgagagcgca ctcacccgcg acatcgagct tcgactgatc
     3181 cctgacacca gcccgcatgc tcgcttctcg ctgcgcagcg tcgagctcgt gtccacgggc
     3241 aaggcatcgg gcacgtttgc cgtcgagcgc accgacagtg atgacatcgt gacccacagc
     3301 gagacaccga acaacccgcc cgtcagccgc atggtctact cccagcggcc aacgagtcag
     3361 gacatgatcg gccaggaaat gcagcgcttc ggcgccgacc cctactatga ggaggccatg
     3421 cggttcgccg tgcgcctgat cccgaactag ggctctgaca ccggccatat cgctctcgca
     3481 gaggctaacc tttaatgaca gcacggccac ctgcaggatg gctcgaccac ggcacgacca
     3541 actcaggcag ggcccagccc gaggccggcg ccgtagcccg ctggctgtcc gtgcacgggt
     3601 gctcctgcga ctgccgcaca agaatgggga gggatggatg cgacgtgtga ttgattacgg
     3661 agagcgtggg caggtcacag tggcccgcaa gtcgagccag ctacccttgc tggcaaccga
     3721 ggagctggcc gtcgtctatc tgcgcgcggc caaccgtccg cagccggcgc tcattacgct
     3781 ttcgggtgga tcgaccccca aggcgatgtt cgggatcatg aaggacccac gtttctggcg
     3841 tcgcatcgag tggtcgaagg tccatctcct gtggggtgac gagcgctggg tgccgatcga
     3901 ggacgagcag agcaatgccg gcgaggcact ccgtggcttc atcgaggaga tgaagctcag
     3961 cgaggagcac taccaccctt ggccgaccca cctcaccgac ccgaacgccg cggccgaggc
     4021 ctatgagatg actatccgca tcctctccgg aacgatggag ggcaccccgg tgctcgatct
     4081 cgtcttcctc ggcatgggcg acgacggcca cactgcctcc atcttccctg gcaccgacgc
     4141 agtgcacgag caggatcgcc tcgcggtcgc ccaccatgtc ccgaaacttg acgccactcg
     4201 cctgacgctg acgcccccca tcctcaacgc cgcgagggag gccgtcttcc tcgtttccgg
     4261 aaccggcaag gccgagatgc tccacaaggt gcttgacggc ccgatcgacg tcgacgcgat
     4321 gcctagccag gcgatccgac ccaccgccgg ccggctccgc tggttcgtcg acgaggccgc
     4381 cgccgcacag ttggaacggg agtaggcgcg atggacacga acacgcggct ggagcgcatc
     4441 ggcgcacagg ccgccggcga ggtgcagccc ggcatgattg tcgggctcgg cacgggttcg
     4501 accgctagcg ccttcgttcg cgccctgggg gcccgggtgg ccgaggggct ctccatcacg
     4561 ggcgtcccga cgtcgaccga gaccgaacgg ctggcccgca gtctcaatat cccgctgaca
     4621 gagctgaacc gcgtcgacgt catcgacctc tgcatcgacg gtgccgatga gatcgacccg
     4681 gcgctcaacg tcgtcaaagg ccgcgggggt gccctgctct tcgagaagct ggtcgcccgt
     4741 cggaccgatc gctacatcat catcgccgcc gacgacaagc tcgtgaaccg gctcggcacc
     4801 cgcctgccac tgccagtgga gatcgtgccg atgggctggc agcacacggc tcgcgagctg
     4861 gagcgcatcg gtctcgggcc ggtcctgcgc accgggacgg atggcaactc gttcgtcacc
     4921 gacagcggcc actacatcgt cgactgcgag accggcggta tcgaagatgc gccggcactg
     4981 gcgagcaaga ttaaggcgat caccggggtc gtcgaccacg gactcttaat cggcctggtc
     5041 gacctcgcga tgacgatcga cgcaacgggg cgtatcacga cacatgaatc agcgattgcc
     5101 tgaaaccctt gccgatctcc cctccccatc aaccagtgcc ggaatcctgc tggtgcttgc
     5161 cggcatcgct tccgtgcttg gcttcttcgc tcgcggagaa caagatattt ggggtgatga
     5221 ggctgtactg gaatcgatcc aggcaatcga cgctccgggg cttgagctgg cggtacgtgt
     5281 atcgaatttc gcattcgaca ccgcgggtgc gctcctggtt gccatcgtgc tggttgcggt
     5341 cgcacttctg ctgcgccggc ccgtgttcgt gcttgagatg gcggtggtga tcgcgttccg
     5401 cgtggcagtg attgtgctca aaccgctctt cgccagccct cggcccggga ccgaacacca
     5461 cgttgacctt gcgcatgtcc ccgaaacctt cggctatccg agcgggcacg ccttcaccgc
     5521 gcctgtcgtc accggcatgt tcgtgctttt tatcgactcg ctcaatctcc cgtcggtgtt
     5581 caaaggcata gccatcgtgc tcgcaaccct cgtgacagcg ctcgggatgt tctcgcgcat
     5641 ctggatcggg gcccactggc cgtcgaacac aattggcgga gtgctctacg ggattgccgc
     5701 cgttgcgctg atgcgcatca tcgcatcgtt catagtgctc caatttggtt ccctattctc
     5761 cacagcagcg agaggccgat cgctccgacg ctgaccagca tcgcgacagc gttgaggtag
     5821 ccatccgatc ggctccgcag cagcttcggc agcagccaca tcttccacct cgagaccggc
     5881 agcagcgggc ggatacctgc atgggtgcaa gcgtctcccg cgagatgcgt cagcagtccc
     5941 gccgcgaagg cgagggtcca aaccagcgcg gtttccggcg acagcgccat cccgcccggc
     6001 aggtcgacgg catcagcgac tgcccgcagc gggagtccaa ccagtgccgt cgcgaagagc
     6061 gcaaacagca ggctgtgcga gatgccgcga tggcgcagcc ggagcggccc gcgaagctcg
     6121 ctccgcagat cgagccagtc cgggaaacca ccaccggcga caccgagcca gaccgcaccg
     6181 atctcgaccg gcaacggcga gggcgcggca accagcaagc cggccgccgc gcccatcatc
     6241 aggtgcgtcg tgcgtttcat cgggcccgga ggctaccagc gatcacgacg cggaccgctg
     6301 cgacccgggc cgctggagcg gcgcccacgc ccctcctcgt cgcgccgtgg gcccccgaag
     6361 cgtcctccgc gtggcgggct gcccggaccg ctccggcgcg ggccgcggcg gtcgtcgcgt
     6421 ccgccagcac cgtccgaggc aacctcgacg ttgaccgggc gtcccttgac gctcgccgcg
     6481 ttcatcgcgt cgatcacctg ttcggccatg ttctccggaa cgtccacgaa gctgaacgcg
     6541 tcgtggatgt cgatcgcgcc gatcgcacgg cccggaatct tcgcctcgtt ggcgattgcg
     6601 ccgacgaagt cctgcgggcg cacccggaag ttgtgcccga tgttgatgaa gaggcgcgtc
     6661 atgccgctct cggcgccacg cgcacggccg cctccggcac cgcgctcacc gctcgggcgg
     6721 ctgctggcat gcgagaggtc atcctgctcg atctcgtcgg tcgcggcgcg accggtctct
     6781 tcggcgtaga gcttgaggat cgcggcggcc aactcgacgg cgtcatgccc gtcggtgagt
     6841 tctcgcaccg gctccaggta ggactcgtag tcctcgcgat catccgcggc gtcgctgacg
     6901 agtttcttga gcatctcgcg gcggcgcttg ttgacgtccg cagcagacgg caacggtcgg
     6961 tactggatct tcgccttggt cacgcgctcg atcgtgcgaa tccagcggac ctcgcggcgg
     7021 gtcaccagcg tgatcgcgtt gccccccttg ccggcacggg cggtgcgacc gatgcggtgc
     7081 acgtaggtct ccggactctc cggaatgtcg aagttgatca cgtggctgac atcggggatg
     7141 tcgaggccgc gcgcggcgac gtccgtcgcg atcaggatct cggcctgacc ggtgcggaag
     7201 cggcgcatca cgcggtcacg ctgcgcctgg ctgaggtcac cgtggattgt ctccaccgcg
     7261 tacccccggc tctgcaacgc ctcgccgagc tggtcaacca tgtgcttcgt gcggcagaag
     7321 atcatcgccg acgacggcac ctcaacgtcg agaatccgtg tcagggcgtc gaccttccgc
     7381 ggttgcggca cctcgtagta ggtctgctcg atctcggcga cggtcatctc cttgccgcgc
     7441 accgaaatgc gatgcggctc gttcatgtac ttggtcgcga gctcggcgat gcgcggcggc
     7501 atggtcgcag agaagagtgc cgtctggcgc tcctccggcg cgtgctgcag cacccactcg
     7561 atgtcgtcga cgaagcccat gtcgagcatc tcgtcggctt catcgagcac gaaaaagtcg
     7621 agagcatcca gcttgagcgt accgcggcgc atatggtcca ttacgcggcc cggtgtgccg
     7681 accacaatgt ggaccccgcg ctggagcccg cgaagctgcc gctcgtaggg ctggccgccg
     7741 tagatggcga gggtttcgac ccgcttgtgc cggccgaatt cctcgagcgc ctgggcgacc
     7801 tgcatcgcca gctcgcgcgt cgggcagagc acgagcgcct gcacctcgcg gcgcttctgg
     7861 tcgatgttct caatgatcgg caaaccgaac gcggccgtct tgccggaacc ggtctgcgcc
     7921 tgggcgatca cgtcgttgcc cgccagcaga gacggaatcg tgcgctcctg gatcggcgtc
     7981 ggggtctcga agccgacggc ggtgatgctc tgcagcaaat catcggagag gccgagcgag
     8041 gcgaaggtga ctcgctgctc gtcgttgtcg gccccttcag cagcgttggt gctgtcgtcg
     8101 cccgtctcgc gatccatcgt ggcagcgcca ccttggtccg cggcgaaaaa cttgaaggtt
     8161 tcggggtcga acgccccgaa gcggttgata acagtcatgg ttgtactcct ggtatgcgtg
     8221 gttctggacc agccaccgtt tctggtgaca cgcacagcag cagaccggcc aacggtgtcg
     8281 gtgtgcgcaa tgcgtatcga atggtcatgg tgtggtgtcg caagaactcg cgaggagtta
     8341 tcccaccgaa cggcttccgt cgacgacagc cacacgccct ggtcgcaccc ctggttggtg
     8401 cgatgcgagc acgatggcgt ccctgaaagg ccgtgtgctc acgtgaccgg gctcgcatga
     8461 ttcgagccgc ggtgtgcttc ccgtgaaccg tgctgcgcag tggggttggt cgctggcgct
     8521 gatgccaggc tgcgcgaggt ctcgtggggg aagtgtcacg agtcggcccg ccggcgatgg
     8581 ccgaggcgga ctcccattct cccgtctatg cgtgaggaaa acgagcgggg tgcgttcgtg
     8641 acttcacgaa gtatagcata aaaccgtccg gacccgcggg cgggccgggc cgcctgtgct
     8701 gttgtcaatg aaacacgtcg ccaccgattc cgccggggag ggacggtggc gacgcggagt
     8761 tgctgtcgat atcgccgaat cgcgacctac gggcgccgaa acccgccagc ggactcgccg
     8821 atatggaagg gcaccgccgg gcgcttgcgc accaccgcgg gccgcgccac ctcggcgcgg
     8881 gcggcattcc gatcgcgctc ctgccgctcg gtggtcagcg cgacgaggcg ctccggcgtg
     8941 atgccgtaca ctgcggcgat tctctccagt tggtcccgtt gggcttgtgt cagttcgtca
     9001 cgtgccggca tcgtcactgc cctttctccg ctgcgcagat caatcaggtc acgtgtatcc
     9061 catcggcacc acgtcgccta tacttacgta ccatactccc tctcccagcc cttccgcaat
     9121 agccgaatgc gcgaattccg ttcgcaccac accagcggtg ctcaccggtc atcaggcacc
     9181 gctgcgaact actgacagac ggcgaatctg ctgcccattg gtgtcgaagt tgtccgggga
     9241 gagcaacgcc tcgaacgacg ccttcaccgc cggccactcc ttgtcaatga tcgagaacca
     9301 ggcggtgcca cgattacgcc ccttgacgat ccggtgctgc ctgaacgtcc cctcgtaggt
     9361 gaacccgaac cgctcagcgc gtaccgagcg cgcgctggca gcgccgcact tccactccag
     9421 gcgccggtag ccgaggtcgt cgaaaacgtg gcgcagcatc aggtagatcg cctcggttgc
     9481 cgccggcgtg cgctgcaacg ataccccgaa gaagatgtgc ccgatctcga tcacgccgaa
     9541 ctcaggctcc atgtgcatgt aactcgcgct gccgaccggc tgtccgctcc tgccatcgac
     9601 cacggtttcc gtcagcattg tcgggtcggc cacgtggcgc gcgagccact gctcgaacgc
     9661 agcctcatcg gcgaacgggc cgtagggcag gtagtcccac aaggcagggc cgccgtcgca
     9721 tgtcgcctcg aacagggcat cggcgtcgcc ggtgacgtcg gtgggacgca cgatcacccg
     9781 gtgaccgtgc aatggctcga cgcttggcag cgagcgcggc gaccaggtcg cgagcgaggt
     9841 atctgtcatg gctgctggtc ctcatcgtcg ccagtttcga gaaagtcatc gatgacccgt
     9901 ttcgggatcc gcagcgggcg cgccgcctcc cgacggatca gcgcccagcg ggtgacggcc
     9961 cgcaccagca cctcgccgct ctccaacgcg gcgacgtccg gcccctcgat cagctccggc
    10021 gtcccgttcg gcgcgatcgc acggcgaatg acgtagcggc gcagcgccgt agccatctgc
    10081 atggaatccg gccaggtgac cacctccaga tggtctccct catatgccgg tcggaggtag
    10141 tcgatctcgt ggcgccgggc gacgaagact ccgccgacct cccgctctag gttctcctgg
    10201 gaccatccca ccagcgcggc gtggtcgatc gctgcctgct ccagccagtt caggtagacc
    10261 gcattgttga catgcccgag cggatcgcac tcgtggaaac gcacgcgcac cggcaatcga
    10321 taggcggtga cgggtcgaac gaggcggctc attcctccat gcgctcctgc tccagatcaa
    10381 agatgcgctg aaggatggcg accgttgacg cgtaggtcga atccatgccg tagaaggaca
    10441 tgccgcgggc accgagcgtg tgggcgcggg tgtacggcgt gtccgaggcg tagtggatga
    10501 tgccgaggtc gatgtggcgg gagagcggcg cgaggttgat cgcctcatcg gtcggatgcc
    10561 gctccagaaa cacatcctcg tagagcgcgc tcaggtacgg cccggcctcc atttcgacca
    10621 ccgtgtagtt gccgcgatag taaaagtcca gatacccctc gttctggagg taggtgccct
    10681 tgactgtcac cgccttctgg ctgtcaagcg cggacccgaa cgtcagatag ggagccacgg
    10741 aatcgtaggt gaatgcgttc tcgaaccagt aggtgttgcc ggagtgctcg tcgaagacga
    10801 cgttggacat catgatgtcg ccgatgcgcc cgttgagcgt cgccgccttg ccgaggatgt
    10861 agacgccgcg ggtgttctca atcgccgcac cgacttgcgt catgatgtga aacgccgtca
    10921 tgccgagcgg gtagttgatg ttgatgatca cggcgtcgga gccgcgcggg tcgagctcgc
    10981 cgagccgtgg gtcgaagagt gaggggtcca gcttcgccag ttcgaagacc tgcacgccga
    11041 catcgaccga tccatcgggt tcgagatacg tgatgccgac ctgctcctcc tcacgctggc
    11101 gctgccggcg ctcctccgcc ctgtgcggtc catagaacca ggagcgcgcc gcaaagtaga
    11161 gcacgttctc ccatgccccc cgcatcttgc cctgctcgcg cttctccaac tccggcagca
    11221 gttcggggtg ctccgtctcg cggatgaact cgaccagctg gcgctcccgc cggatcgccg
    11281 tgccggagaa cacgttggcg atgctgtggg tgttggagga cacgaagtag gtcggccgcc
    11341 cgagcagccc gcgcgccgcc agcacgtcgt agaccggccg ccaccacgcc cgaacggagc
    11401 gcccatagcc gagataggtc ccgccgagca tctggatgtc aatgcgcagg tggtccgtcg
    11461 ccatccgctt caggttcggc atcaggtcgc cgccccacac gcgctgcagg cgctcgaaat
    11521 cgggctcgga aagatacagt gccttccgca ccgcggggtc gtccgccgcg atcggctcgc
    11581 ccttgcccag caaccggtcg cgcacgtcgg gcgattcgtt gagcagatca tgcatcttgt
    11641 tccactcgat ctcgtaggcc acgatcgagg ggatgagatc atcgaggtcg gaggcggagg
    11701 agatgtaggc gcccagcgtg tcggtgccgt tgaagtacca gcgccgccgg cgggctgggg
    11761 cgctgacagc ctgccatgac tcgatctcag gatagccgcc gcggctgaag gcccggtgcg
    11821 actggccgag gatgatgtgc ttgacctgcg gaaggcaggc ggggatgcga ttgacggagt
    11881 agaggaaggc gctgaggtcc ggttcccgct cctcggcacc ggcgtgcagc gaggaggacg
    11941 cagtcaggtg cgccggctcc agcgaggaga cagagacagc gccagagctc tccagcaggg
    12001 tgttgtaggt gcgaacgtag agatcaacct cgcgcttgcc gggccgccgc tcaacgcgca
    12061 tgtcgtcgtg gtcgtgccac tcgtccatct gccggtgact cgtcatgttt cgctcctcgc
    12121 cccctgtgtc gccgtcgcgc tgcggccgct gcgcatccgc cgggcggaca cgaggtccgt
    12181 ccctacggga acctacccca aaatctccac cccgaaggcg cgcaggaact ccagcgcgat
    12241 caccgcgtgg cccggtccgt tcgggtggac cttgtcgtcg gcccagtcac tgctctcgct
    12301 gtgctccagc acacggtcga acgccgcctg cacgtcgatg aagaccgcgc cgacttcctc
    12361 ggccacctcc cgcgccagca ggccatagag gtccatcgcc gagcgcatca ggtcgctcgt
    12421 gtcggcttca atcatatagg gagagccgac atagagatca ggcgcgcccc gctccctggt
    12481 ggcgcgcagc aacgtgacca aggtcgactg gtactcgtcc gccggcacgg cttcgagtgg
    12541 gttgtcgccg aagcgccgcc aaacgtcgtt gatgccgatc atcacggtca gcacgtcagg
    12601 tcgctcgtcg agcacgtcac tcacccagcg gtcggcaagg ttccggacgg tatcgccgcc
    12661 gatgccccgg ttctcccacg tcaggtccag ttcggggtag agcgccgtgg cgatgctgcg
    12721 caccagcgag acgtagccgg cgccgtaggg tgggtcgatg tcccgccgcc cggcatcggt
    12781 gatgctgtcg ccgaccatga cgatcttctg tcccgttttc agtctcacgt tagtctcctc
    12841 gagttctcgc tcgttcccac agggccgaac ctataggcta tccgccgggc cgcagggcgg
    12901 catcatgtcg gggcatgcaa acccatctcg tccaatgatc caagctgtca ccctgggcga
    12961 aggccgaaag ccggaatcga aggatctctt gcgtcatggg tagtggctct cagcgagcaa
    13021 gcgattcctt cggcttcgct caggacaggc tcttcgacac acatccgctt cgctcggtgc
    13081 aggcactggt gccacaggcg gcttgccagc tcaggatgac agcatgaatc gcttgcgggc
    13141 ggacgactac gtgcgagaag tccaccctgt tgattgtgcg atccattgct caacgagtgt
    13201 cgccatcggc ccgcatcgac gtccaggatc tatcgttttg gacagcaacg cccgattccc
    13261 ctactcttga cacatccgcc gcatcgactc gacggcaccg tcgcccccgc cagcgacctc
    13321 gccaaacccc tgccctgccg cacatgccgc atcgcatctt gcccatcacc atagccatcg
    13381 gcggcatcag tcacgagacc catgccttcg ctgggcagct gaccaccctt gccgacttcg
    13441 acggacgcgc cctgctcacc ggcaaccgcc tgcttgagca cgccaggggc agcgatagcg
    13501 tgctcggcgg catcatcgcc gccgctcgcc ctgacgtcga gttggtcccc accctcttcg
    13561 cctcggcgat gcctggcggt ccggtggtcc acgaggcatg ggatgacctc agccggcgcc
    13621 tcctcacgcg cctgcgcacg gcggcgatcc gcccgcctgc catcgatggc gtcgtgctcg
    13681 ccctccacgg cgcgatggcc accaccgaga gccgcgaccc cgacagtgca ctggtcctcg
    13741 ccgtgcgcga gatcgtgggc gatggcgtcc ccatcgtcgt cgtgctcgac gcgcacggca
    13801 cgccctccga ccgcctgatc gagacggcgg acgcagtcct cgcctaccgc acctatccgc
    13861 acatcgacat gcaggcgacc ggcgcggcag ccctcgccgt ttgccgatcg ctcgtgaatg
    13921 gcgacgtgca gccgcgcacg gcggttcggc gcctgccggt gctgctgcca ctcacggcac
    13981 agcgaaccaa cggcccgacc ccgatgacgc gcccggtgcg gcaggccgtc gcactggagc
    14041 gactcccggc ggtgctgcag gtcaacctgc tgcctggctt ccccttcaac gatgtgc
//
LOCUS       DASYWL010000334         2147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_261945, whole genome shotgun sequence.
ACCESSION   DASYWL010000334 DASYWL010000000
VERSION     DASYWL010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261945"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VGR22_11760"
     CDS             complement(<1..215)
                     /locus_tag="VGR22_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129284.1"
                     /translation="MTQSRSASSIAGVDDNAETMPGMMGVGMSSTLRRGAWWRSALGW
                     TWRLGLLLPLLFAAGVLIFFFGVAGTR"
BASE COUNT          359 a          689 c          703 g          396 t
ORIGIN      
        1 aaccgggtgc cggcgacacc gaagaaaaat atcagtactc ctgcggcgaa cagcaggggc
       61 aggaggagtc ccagccgcca cgtccaaccc agcgcggacc gccaccacgc tccacgtctc
      121 aaggtggagc tcataccgac acccatcatg ccgggcatgg tctctgcgtt gtcgtcgacc
      181 cctgcgatcg agctcgccga gcgactctga gtcataacca ccgcccaccg tcgacggcgc
      241 tgaccagctc gtctcagatt gccggctcgc cgacgggcgg cgccagcgcg aagaagcgtt
      301 cggcgttcct gaccctccac gccgggcggt cggacgccgc cgctcgagac ccccgcgagc
      361 ggcacctcgg tacggctcgt cagcgcgatc gcacggtgca cagcgcgatc atcttccgag
      421 tgcattacga accgactcgc cgcgaggcga gattgttgat cgcggcggcg ctgtgcttct
      481 tgagctggct ggcgtcatgg ctcctggcgg cgatactgcc gtggaaggtg cttggcgccg
      541 ccctcatcgg agggctcggg atcgcttgga tcgcctcgct ggcggtgtgg gagttgtcag
      601 cgacgcgccg acttctcggg aggcgagttc gcatctcgga cttggcgtgg caaacaaggc
      661 tgcgccttaa cctcttgtca ccgcgggtct ggcgtctggc gctcgatgta gcgcgcaacg
      721 gctgaagtcc ggcccgtggc aatcgaggtg gcggacgcca gccgatcgac gcggatattg
      781 ccgggccgcg agcgcgcgag gccagcgcga acaaaccttc ggagctactc accctcgacg
      841 tcgagccgtc ggccgccctc gctcgaggcc aaggagatcg gcacgtagga gcggctgccg
      901 aggtgtgggc aaagggtgat taggttggca gcacgaccgg agccacggct acagccctgc
      961 tcaagtcgag tccgtgctcg accgcttacc ggtgggagag gaccccccct tcttcgtcgt
     1021 gcgggcgacc attgtgcgct ccgtggtcag gctgggatcg gagccttcct ccgactcgtc
     1081 cagcaagagc gagggatcac tctgtcatgc catcacttcg gtcgccgcac tgtgttggca
     1141 ctgtgttgac ggcgtacgtc tcgacggctg ggtcggtgtt gacgccgttg atcttgagga
     1201 tgcgctcggt tttgcttatg gagccgcgcg agtcgctgct agctcgcctc ttgccgcagc
     1261 gggtatcttc tttatcggct atggcattgc tgcttcgtgc tgactaaacg agcgaggttc
     1321 gtggaggaca cgacatcatg cggacaccca agcggatagt gcagtggatc gttgtcgttt
     1381 tattaagtct actcgccatt agggcgctgg tattcacctt ttcatctgta cggagcgggg
     1441 actcaaagtc aatagcagtt ggcgtggcca tttctctatt tcagatgcta gtgctggcgg
     1501 gagccattgc ctggttgaag accgcccaga gcggcgatcg gtaggctcgg ctccgatgcc
     1561 aggcgtcgcc ggacatcccc aagcgcaacc gtcgaaccgt cggagcaggg agcggctgcg
     1621 ggagatgctg gcgattaatt tgccaaagag cgctcgaggt ccgcgacgcg ggacacgggc
     1681 taacccgccg gaatgttcgg tactcctcgg tgccgccggc gccggccctc cgctccggcg
     1741 gtccagcccc acgccgaccg gcacttcggc actcatcgca gcaggagcga gaggggtgcc
     1801 agaacggcga ggactgcgac ggcgatggct gctagccagg ccttccccgt gccggaaaaa
     1861 cgccatctcg cgtgcttccc acgatcgtcc tccgtggtgc ctggccgctt ggatcatgcc
     1921 cactgccagt gccgccaata cgacgaattc gacagccgcg gcgaagatcc gcaccgggcc
     1981 ttcggtagtg tcgccgaaag cgtgggctgt tgcggctacc gcagaggcac cgaaggcggc
     2041 gatcacgttc tttacgcgga tcgtcggccg gtatcgagcg ggcccagcat gcgtgagccg
     2101 ggtcttctct agccagtcat ccagccagcc cggcatcatc tccagtc
//
LOCUS       DASYWL010000335        11120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_524377, whole genome shotgun sequence.
ACCESSION   DASYWL010000335 DASYWL010000000
VERSION     DASYWL010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 11120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11120
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524377"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2205
                     /locus_tag="VGR22_11765"
     CDS             <1..2205
                     /locus_tag="VGR22_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEV2129285.1"
                     /translation="RGIDTSAAKEMPGVVAVLTYDDLARILVDVETETKGESTGEDSM
                     EEEFEDIPVPPILPLAQSRVRYVGQPVAAVIAETQAQAEDAVELIDVDIEPLDVVTDV
                     FEAMEDGAPQLFDEVPNNIAVRFGGERGGDVEAAFASAPVKIKQRIRSQRVNAVPMEP
                     RGVVATKDPISGGLTFWTSTQAPHWNRNAVAKALGLSQGKVRCIAPEVGGGFGQKIGA
                     YPEDYVVTAAAWNLGRPVKWIETRSENMLSSSHGRNQFADMEAAADRDGKILGLRARV
                     YLDCGGYPKGLDLGNATWIMATGCYDIPAIDYRVTGVYTNTGGNGAYRGAGRPEAAYY
                     IERVVDLVADEAGLDPVEVRRRNFIQPEQFPFRTLTGLRYDSGEYEKPLNKALGIADY
                     EGLRKQQAELREQGRYLGIGLASYVEICGFGPYESSTVRVEPSGDISVFTGISPHGQG
                     QETTFAQMAADSLGADYDTIVVHHGDTGNTPQGNGTMGSRGLAVGGAALMISLNKIQE
                     KAKRIAAHMLEAAIEDIEIQDGRYRVKGVPEGGVTLGEIADLAYSGDLPDEIDAGLET
                     TDFFRPSDTTFPFGTHVAVVEVLPETGEVKWHRYISVDDCGPIVSPMLVTGQVHGGLA
                     QGIGQALLEEMHYDSSGELITGTMNDYTVPKAHHFPNFESHHTETPTDVNPMGVKGIG
                     EAATIGATPATANAVIDALEPFGIRHMDVPLMPQKIWKAIQDAQGGSSQAAD"
     gene            2432..2527
                     /locus_tag="VGR22_11770"
     CDS             2432..2527
                     /locus_tag="VGR22_11770"
                     /inference="COORDINATES: protein motif:HMM:NF015060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HEV2129286.1"
                     /translation="MNAKLAALLLVPYLLWTPFAAILNPQHVDLV"
     gene            2708..3652
                     /locus_tag="VGR22_11775"
     CDS             2708..3652
                     /locus_tag="VGR22_11775"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV2129287.1"
                     /translation="MAALNAGLSVPPTPDSQHDLTVIGELDEAGVQTAWQTAGGTRPD
                     STTLMAAAAVTTNAIRLGTSIVPTYPRHPTVLANQAKVIESLAPGRLRLGVGPSHRPT
                     VEGRLGIPMGKPLTHLREYVTVLRGLLKDEDVDFHGEYFNVSMPGSPDPAPVAPEVLV
                     SALSENAFRLGGEIADGAISWVAPVPYLVGTALPALEEGAAKANRAAPPIIAHVPVAV
                     TRDRSSALKASSTMFGAYGSLPFYAKMFAAAGVPVGADNRTSPEAIDVLTVSGSPDEI
                     RTRLEAIIAQGIGELLISHVPIDDEAAERRELARILAG"
     gene            complement(3986..5830)
                     /gene="treZ"
                     /locus_tag="VGR22_11780"
     CDS             complement(3986..5830)
                     /gene="treZ"
                     /locus_tag="VGR22_11780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEV2129288.1"
                     /translation="MTRLMEMGANLTGDGCRFDVWSTNAVDVVVELIQDDRVSTHPME
                     ACGDNHFRAEIAGVRAGDRYRFRLDGAGGLPDPYSRFQPEGVHGPSEVIDPSSFSWSD
                     DGWSGLSAANLAIYELHVGTMTPEGTYAGLEAELEAIRDLGVTAIELMPVAQTPGTRN
                     WGYDGVDLFAPNHVYGRPDDLRRLADAAHRVGLGVILDVVYNHLGPEGAYLSAYSDAY
                     FSETHQTSWGAGLNWDGPHSHWVRQFAVDNACYWISEFHIDGLRLDATHAILDDSQKH
                     IVQELTEAARDATGDREIVMIAEDERHDVTRVRLATRGGEGLDGVWADDFHHEVRVLL
                     TNAHESYYALYDGTTEDIAHAVQNGFGLEQPEPEAQPDASDAASAFVFCIQNHDQIGN
                     RPFGSRLHHEISEDRYRVASALLLFAPETPLLFMGQEFAASTPFLYFTDHPPELGKLV
                     TEGRRKEFAGFRAFDDPRLRDTIPDPQAESTFLVSKLDLSERETNADVLELYRTLLRI
                     RREDPVLTIQDFRRTDAFAPSAELVVVHRWNGIEHRVLVANFGHRTEMPATWLEAVEG
                     GPGERWEMMLSTSEERFGGGGQRPEIDIGARSLTMPARTAAMFRFGHH"
     gene            complement(5827..8823)
                     /gene="treY"
                     /locus_tag="VGR22_11785"
     CDS             complement(5827..8823)
                     /gene="treY"
                     /locus_tag="VGR22_11785"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEV2129289.1"
                     /translation="MTLTTIETDPVIRQMLDHPDTGTPPRAAYRLQFNHTFTFDNAAA
                     IVPWLAELGVSHLYASPIFRATPGSMHGYDVVDYGEINPEMGGREAFGRLVSVVLEHG
                     MKILVDYVPNHMGIEGGANAGWQDVLEHGPASPYESYFDIDWSPIMSELRDKVLLPVL
                     GDQYGVVLENGELNVRYDAGTFTVWYWETPLPVNPVTYPVLLRPVLERVSETLDEDDL
                     DRLELESIITAFERLPEMETSDVPGEHDPRYREQLVTRRRLEDLAERSDVVCQAIEDT
                     LVAFNGEVGDPRSFDRLDNLLGQQSWRVAFWRVAGEEINYRRFFAINTLAAIRQEEPR
                     VFRETHRLLMQLLAAGAINGVRIDHPDGLWDPEGYFFDLQAEFLAVSSLREQGVEPDH
                     LYEEEHVETLERLRAQARIAVEEGAAERQQWPLYVLAEKILEHGEALPETWAIAGTVG
                     YDFAHATTALFVDTASRQMFDRIFSRFTGDSIRFPEMVYRMKHQMMDEAFQSEIAVLS
                     NALNRIAEEDRRYRDFTLNNLRAALTEIMASFPVYRTYTTCADEGVEERDHRYIDEAV
                     AAARRRSSGLDPSVFDFLHEVLVLHVSQEPGAISRFNRRCYFAMKFQQLSGPVMAKGL
                     EDTAFYRYNRLVSLNEVGGDPSHFGTNIEEFHRQNRVRLRRWPYAMLNSSTHDTKRSE
                     DVRARISTLSHDPVTWRAAINRWSRLNRKLKRKVDLALAPYRTDEYVIYQSLVGTWPL
                     EGYRETDRAVYVERLQEYMVKVVREAARFSNWVNPNEEYEAALRAFIAGLFNLRRSAR
                     FLDDIEAFVQERLTTGLQHALAAQVLKLTSPGVPDIYQGTEVWDDSLVDPDNRRPVDF
                     ERISALANRYDDIDGAWEQRLDGAVKVMTTRALLQARSEHPKLFLTGEYQALEVGDVP
                     GLAYLRVRDDRALLVAVGWSPASLENGTVELPERWRSAHWSDVLRDGSAVQVREGGDL
                     PVGTGEGRDGMPVRVLMGVIDEGGDA"
     gene            complement(8864..10324)
                     /gene="argH"
                     /locus_tag="VGR22_11790"
     CDS             complement(8864..10324)
                     /gene="argH"
                     /locus_tag="VGR22_11790"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEV2129290.1"
                     /translation="MTDNGGAGDVRAKAWGGRFMEVPDARLEAFNASVTFDIRLVSED
                     IRGSIAHARMLGAQGIIPVDDAEALELGLWRILDEVEAGEFVLTISDEDVHTGVERRL
                     RELVGQTAGKLHTGRSRNDQVVNDVRFWTRQRLASIVDGLNNLCDALLDLGERYRDAI
                     MPGYTHLQRAQPVLVAHHMQAYVAMFQRDIERIRQAWARTNVLALGSAALAGTTYPID
                     REMVAKDLGFAEVSINSLDAVSDRDFVIDTLSASAVIGMHVSRLSEEVILWTSGEFRF
                     MELADRFSTGSSIMPQKKNADIAELGRGKSGRLYGNLMALLTITKGLPLAFNKDMQED
                     KEPLFDSIDTVLAILEVFPPMMQSATFDKERMATAAVADFTLATDAADLLAKHGVPFR
                     EAHHVVGSLVGKCVSHGKTFADLTDEEWTEIHPVFSEARPPVDAWTSVAGRDSLGGTS
                     PAQVERTRGLQREALESSRQWVAERLAAERNLFQRS"
     gene            complement(10530..>11120)
                     /locus_tag="VGR22_11795"
     CDS             complement(10530..>11120)
                     /locus_tag="VGR22_11795"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV2129291.1"
                     /translation="RSLYRLAIDTGLRQGELLALQWGDINLERGILMVTRNLTGSRAE
                     YDDAGNKIAGALQFTTPKTKAGVRIVPFPLASAEALRNQQAYVDQLRDAATTWTEHDL
                     VFPSRVRTPLTPTNVGNRFRQRREAAGIPDAVFHSFRHTATSRLMLDNIPHPIAMALL
                     GHKNIDTTMLYTHASEEKLIETMRAYFETKTDPEDA"
BASE COUNT         2022 a         3647 c         3542 g         1909 t
ORIGIN      
        1 cgcggcatcg acacctccgc agcgaaggag atgccgggtg tggtcgccgt gctcacctac
       61 gacgaccttg ccaggatcct cgtcgacgtc gagacggaaa ccaagggcga gagcacgggt
      121 gaggattcga tggaggagga gttcgaggac atccccgttc caccgatcct gccgctcgcg
      181 cagtccaggg tgcgctacgt cggtcagccg gtcgccgcgg tgattgccga gacacaagcg
      241 caggcggagg atgccgtcga gctgatcgac gtcgacatcg agccacttga tgtggtgacg
      301 gacgtcttcg aggcgatgga ggacggagcc ccgcagctct tcgacgaggt tcccaacaac
      361 atcgctgtcc gcttcggcgg cgagcggggc ggtgacgtcg aggctgcatt cgcgtcggcc
      421 ccggtgaaga tcaagcaacg catccgcagc cagcgcgtca acgccgtgcc gatggagccg
      481 cgtggcgtgg tcgcgacgaa ggaccccatc agcggcggcc tgacgttctg gaccagcacg
      541 caggcgccgc actggaaccg caacgcggtc gccaaggcgc tcggcctgag ccagggcaag
      601 gtccgctgca tcgccccgga agtcggtggc ggcttcgggc agaagatcgg cgcctacccc
      661 gaagactacg tcgtcaccgc ggcggcctgg aatctgggac ggccggtcaa gtggatcgag
      721 acccgctccg agaacatgct ctccagcagc cacggccgca atcagttcgc ggatatggaa
      781 gcagccgccg acagggacgg caagatcctc gggttgcgcg cccgcgtcta tctcgactgt
      841 ggcggatacc cgaagggcct cgatctcggc aacgcgacct ggatcatggc gacgggctgc
      901 tacgacatcc cggcgattga ctaccgggtt acgggggtct acaccaacac aggcggcaac
      961 ggcgcctatc gcggtgcggg tcgcccggaa gccgcctact acatcgagcg cgtggtcgac
     1021 ctcgtcgccg acgaagcggg gctcgacccg gtcgaggtcc ggcggcgcaa cttcatccag
     1081 cccgagcagt tccccttccg cacattgact ggtcttcgct acgacagcgg cgagtacgag
     1141 aagccgctca acaaggccct ggggatcgcc gactacgagg gattgcgcaa acagcaggcg
     1201 gagctgcgtg agcagggccg ctacctcggc atcggtctcg cctcctacgt ggaaatctgc
     1261 ggattcggcc cgtacgagag ctcgaccgtg cgggtggagc cgagcgggga tatctcggtc
     1321 ttcaccggca tctccccaca cgggcagggg caggagacga ccttcgccca gatggcggct
     1381 gacagcctcg gcgcggatta cgacaccatc gtggtccacc atggtgacac cggcaacacg
     1441 ccgcagggca acggcacgat gggcagccgc ggcctcgcgg tcggtggcgc ggcactgatg
     1501 atctccctca acaagattca ggaaaaggcg aagcggatcg ccgcccacat gctggaggcg
     1561 gcaatcgagg atatcgagat ccaggacggc agataccggg tcaagggcgt acccgagggc
     1621 ggcgtgacgc tcggcgagat cgccgacctc gcctacagcg gtgacctgcc ggacgagatc
     1681 gacgccggcc tcgagacaac cgacttcttc cgcccgtcgg acaccacgtt cccgttcggc
     1741 acgcatgtcg ccgtagtcga ggtgctgccg gagaccggcg aggtgaaatg gcatcgctac
     1801 atctcggtcg atgactgcgg accgatcgtc agcccgatgc tcgtgaccgg gcaggtgcat
     1861 ggcgggctcg cgcaaggcat cggccaagcg ctgctggagg agatgcacta cgactccagc
     1921 ggcgagttga tcactggcac catgaacgac tacacagtgc caaaggccca ccacttcccg
     1981 aacttcgagt cgcaccacac ggagacgccg acggacgtca acccgatggg cgtcaagggt
     2041 atcggcgagg cggcgacgat cggcgcgacc ccggcgacgg cgaacgcagt gatcgatgcg
     2101 ctggagccgt tcggcatcag gcacatggac gttccgctga tgccccagaa gatctggaag
     2161 gcgattcagg acgcgcaggg cgggtcctca caagctgccg actagcggca gcatcacagg
     2221 agtgcgagca ggaaggtggc acggacgatg agtccgcgcc accttcccgt ccaacgcgtc
     2281 aagccggcaa agacagcgaa cgaaaggtag ccccgccgcg aaagtgggcg gggagcgtcg
     2341 gcgcagccaa gaaccgtgca ccggcaaact cacgctaacc cggacatcga aatcctggtc
     2401 tccatgctgg cgacgccttg ctcagcagtt ggtgaacgcg aagctcgcgg cgctgctgct
     2461 ggtgccctac ctgctgtgga cgcccttcgc cgcgatcctc aatcctcaac atgtcgatct
     2521 ggtataagac cgaaaggacc gagcactgag cggaaccctg catcaggttg aggccgcaac
     2581 gccgccgcct tgctcgtgtg cctgtcctgc ctgaccagtt cctgccgcct tggctaccct
     2641 gccgcgaatg ctgatctcat gcccccaccg tccgttgcga cgatggaaga taccggggaa
     2701 gttccagatg gctgcactca acgcgggact gtcggttccg ccgacaccgg attcccaaca
     2761 tgacctcacc gtgatcggtg agctggatga agcgggtgtg caaaccgctt ggcagaccgc
     2821 gggcggcacg cgtccggact cgacgacact gatggcggct gccgcagtta cgaccaacgc
     2881 gatcaggttg ggaacgtcaa tcgtgccgac ctatccccgc catcccaccg ttctggcgaa
     2941 ccaggcgaaa gtcatcgaga gcttggcacc cggccggctg cggctcggcg tgggcccgag
     3001 ccaccgcccc accgttgagg gcaggctcgg cataccgatg gggaagccgc tgacgcatct
     3061 gcgggaatac gtgacggtgc tcaggggcct cctgaaagat gaggacgtcg acttccatgg
     3121 ggagtacttc aacgtatcaa tgccggggtc cccggatcca gccccggtcg cgccggaggt
     3181 cctcgtctcg gccctgagtg agaacgcgtt ccggctcggc ggcgagatcg cggatggggc
     3241 catcagctgg gtcgcacctg tcccctacct cgtcggaact gcgctccccg cccttgagga
     3301 aggcgcggcg aaagcgaatc gcgcggcgcc cccgatcatc gctcacgtgc cggtggccgt
     3361 gacacgcgac cgatcgagcg ccctcaaggc atcgagcacg atgttcggcg cctacgggtc
     3421 gctgcccttc tacgcaaaga tgttcgcggc cgccggcgtc ccggtcggtg ccgacaaccg
     3481 cacgtcgccg gaggccattg acgtgctgac ggtttccgga tcgccggacg aaatcaggac
     3541 acgtctcgaa gcgatcatcg cccagggtat cggcgagttg cttatatcgc acgtcccaat
     3601 cgacgacgaa gccgccgagc gccgggagct ggcccggatc ctcgcggggt gacagaccgg
     3661 ataccgcact ccggggagca tcgatccggc cccctacggc caccctttct cttggagcgg
     3721 gctgacaacg gcctcgttga gcactagcgc ctgcggcgcg gtggcggtcc agacgaccaa
     3781 ggcggccaca ccggtggcgg cttcggcaag agcacatgcc ctcgcgcggc ccaacccact
     3841 gctcgctccc gtcacgagcg caacctgtgc agacagtgtg cgccgatcca cctctgactg
     3901 catctgctac cccgatcacg gctctgacca gtgaacgcca ccatgctgcc acgtccccgc
     3961 cacgtccgcc gtacggcgag gcttgtcagt gatgcccgaa ccggaacata gcagctgtgc
     4021 gggccggcat cgttagggaa cgagcaccga tgtcgatctc cggccgctgt cccccgccgc
     4081 cgaatcgttc ctcgctcgtt gagagcatca tctcccacct ttcgcctggg ccgccctcga
     4141 ccgcctccag ccacgtcgcg ggcatctccg tccggtggcc gaagttcgca acgagcacgc
     4201 gatgctcgat gccattccag cgatggacga cgaccagttc agcgctcggc gcaaacgcat
     4261 ccgtccgccg gaaatcctgg atcgtcagca ccgggtcctc gcgccggatc cggagcaggg
     4321 tccggtagag ctcgagcacg tcggcgttgg tctctcgctc ggagagatcg agcttcgaga
     4381 cgagaaaggt cgactccgcc tgcgggtcgg gaatcgtgtc gcggaggcgc ggatcgtcga
     4441 acgccctgaa accggcaaac tccttgcggc gcccctccgt caccagtttc cccagctcgg
     4501 gcggatggtc ggtaaagtag aggaacggcg tcgaggcggc aaattcctgt cccatgaaga
     4561 gcagcggcgt ctccggcgcg aagagcagca acgccgatgc cacccggtaa cgatcctcgc
     4621 tgatctcatg gtgcagccgg ctgccgaacg ggcggttgcc gatctggtcg tggttctgga
     4681 tgcagaagac gaatgccgag gcggcgtctg acgcatccgg ttgagcctcc ggctccggtt
     4741 gctccagccc gaagccgttc tgcaccgcgt gcgcgatgtc ctcggtcgta ccgtcgtaga
     4801 gcgcatagta gctctcgtgg gcatttgtca gcaggacacg cacctcgtga tggaaatcgt
     4861 ccgcccagac gccatccagc ccctcgccgc ccctcgtcgc cagccggacg cgggtcacgt
     4921 cgtgtcgctc atcctcggcg atcatgacga tctcgcgatc acccgtagcg tcgcgcgccg
     4981 cctcggtcag ctcctgcacg atgtgtttct ggctgtcgtc gagaatcgcg tgggtcgcgt
     5041 cgagccgaag cccgtcgatg tggaactcac tgatccagta acaggcgttg tcgaccgcaa
     5101 attgccgcac ccagtggctg tggggtccgt cccagttcag cccggcgccc cacgacgtct
     5161 ggtgcgtctc actgaagtag gcgtcggagt atgcggaaag gtaggcacct tccgggccga
     5221 ggtggttgta gaccacgtcg aggatcacgc cgagccccac acgatgcgcc gcatcggcga
     5281 ggcgccgcag atcatcggga cgcccgtaga cgtgattcgg cgcgaagagg tcgacgccgt
     5341 cgtaccccca gttgcgcgtt cccggtgtct gggcgacagg catcaactcg atcgccgtca
     5401 cgcccagatc gcgaatcgcc tccagttcgg cttccagccc ggcataggtc ccctcgggag
     5461 tcatcgtgcc aacgtggagc tcatagatcg ccagatttgc ggccgagagc ccactccaac
     5521 cgtcatcgga ccaactgaag ctggaagggt ctatgacttc cgagggacca tggaccccct
     5581 ccggctggaa ccgtgaatac gggtccggaa ggccgcctgc accgtcaagg cggaaccgat
     5641 agcgatcccc tgcccttacc cccgcgatct ccgcccggaa gtgattgtct ccgcacgcct
     5701 ccattggatg cgtcgagacg cgatcgtcct ggatgagttc aacaacgacg tcgactgcat
     5761 tggtcgacca gacgtcgaac cggcagccgt cgcccgtgag gttggccccc atctccatca
     5821 gccgggtcat gcatcgcccc cctcgtcaat cacacccatc aacacgcgta cgggcattcc
     5881 gtcgcgcccc tcacccgtcc cgacgggcag atcaccgccc tcccgaacct gaacggcact
     5941 accgtcccgg agcacgtcgc tccagtgcgc ggagcgccag cgctccggca gctcgacagt
     6001 tccgttttcg agcgacgccg ggctccagcc gaccgcaacg agcaaggcgc gatcgtcacg
     6061 tacccgcagg tatgcgagtc ccgggacatc gcccacctcg agcgcctgat actcgcccgt
     6121 caggaagagc ttcggatgct ccgatcgcgc ctgcagcagc gcccgcgtcg tcatgacttt
     6181 cacggcaccg tcgaggcgtt gctcccaggc tccgtcgatg tcgtcgtatc ggttcgccag
     6241 cgcggagatg cgctcgaagt cgacgggacg ccggttgtcg ggatcgacca gcgagtcatc
     6301 ccagacctcc gtcccctggt agatatcggg cacacccggg ctcgtcagct tcagcacctg
     6361 cgcagcgagc gcgtgctgga gtccggtggt gagccgctcc tggacgaagg cctcaatgtc
     6421 gtcgaggaac cgggccgagc gccgcagatt gaagagcccg gcgatgaacg cccgcagcgc
     6481 cgcttcgtac tcttcgtttg ggttgaccca gttggagaag cgcgccgcct cccgcacgac
     6541 cttgaccata tactcctgca gccgctcgac gtacacggcc cggtcagtct cgcggtaccc
     6601 ctcaagcggc cacgtgccga cgagggactg gtagatgaca tactcatctg tccgatacgg
     6661 agcaagcgca agatcaacct tgcgcttgag cttgcggttg agccgtgacc agcggttgat
     6721 cgctgcccgc cacgtaacag ggtcgtgact caaagtgctg atccgtgccc ggacatcctc
     6781 gctgcgcttg gtgtcgtggg tagaagagtt gagcatggcg tatggccacc tgcgcaaacg
     6841 cacccggttt tgccggtgga actcctcgat gttggtgccg aagtggctcg gatcaccgcc
     6901 tacctcgttg agggacacca accggttgta gcgatagaag gcggtgtcct cgagcccctt
     6961 ggccatcacc ggaccggaga gctgctgaaa cttcatcgcg aagtagcagc gccggttgaa
     7021 gcgggagatt gcgcctggct cctggctcac gtgcagcacc agcacctcgt gtagaaagtc
     7081 gaagacggat ggatcgagcc cggaactccg gcgtctcgcc gccgccacgg cctcgtcgat
     7141 atagcgatgg tcgcgctcct cgacaccctc gtcagcacag gtcgtatagg tccggtagac
     7201 ggggaaggaa gccatgatct cggtgagggc agcccgcagg ttattcaggg tgaagtcgcg
     7261 gtagcggcgg tcctcctcgg cgatacgatt gagcgcgttg ctgagcaccg caatctcgct
     7321 ctggaacgcc tcatccatca tctggtgctt catccggtag accatctcgg gaaagcggat
     7381 ggagtcgccg gtgaaccggc tgaagatgcg atcgaacatc tgccgcgacg cagtatcgac
     7441 gaagagtgct gtcgttgcgt gcgcgaagtc gtacccgacc gtgccggcta tcgcccaggt
     7501 ttccggcagc gcctctccgt gctccagaat cttctcggcg agcacgtaga gcggccactg
     7561 ctggcgttcg gccgctccct cctcgacggc aatacgtgcc tgcgcccgca gccgctccag
     7621 tgtctcgacg tgctcctcct cgtagaggtg gtctggctcg acgccctgct cgcgcagcga
     7681 ggagacggca aggaactcgg cctgaaggtc gaagaagtag ccttccggat cccagagacc
     7741 atcaggatgg tcgatgcgca ccccattgat cgcgcccgcc gccagcagct gcatcaacag
     7801 ccgatgcgtt tcgcggaaga cccgcggctc ctcctgccga atcgcggcaa gcgtgttgat
     7861 cgcaaagaag cggcggtaat tgatttcctc tccggcaacg cgccagaacg ccacccgcca
     7921 cgattgctgc ccgagcaggt tgtccaaacg atcgaatgac cggggatcac caacctcgcc
     7981 gttgaaagcg acgagcgtgt cctcgattgc ctggcagacc acatcggatc gctcggcgag
     8041 gtcctccagc cgccgtcgcg tgacgagttg ctcgcggtat cgcggatcat gctcccctgg
     8101 aacgtcgctg gtttccatct cgggtagacg ctcgaaggcc gtgatgatgc tctccagctc
     8161 gaggcggtcg agatcgtcct catcgagcgt ctcgctcacc cgctccaaca ctggccgcag
     8221 cagcaccggg taggtgaccg ggttgaccgg cagcggtgtc tcccagtacc agacggtgaa
     8281 ggtgccagca tcgtagcgga cgttcagctc gccgttctcg agcacgacac cgtactggtc
     8341 gccgaggacc ggcaggagca ccttgtcgcg cagctcgctc atgatcggcg accagtcgat
     8401 atcgaagtag ctctcgtacg gcgaggcggg accgtgctcc agcacgtcct gccacccggc
     8461 attggcgccg ccctcgatcc ccatgtggtt cggcacgtag tcgacgagga tcttcatgcc
     8521 atgctcgagg acgaccgaga cgagccgacc gaatgcctcg cgaccaccca tttcggggtt
     8581 gatctcgccg tagtcaacga catcgtatcc atgcatcgag ccaggcgtcg ctcggaagat
     8641 cggcgaggca tagaggtgac tcacgccgag ctccgccagc cacggcacga tggcagcggc
     8701 gttgtcaaag gtgaacgtgt ggttgaactg caggcgatag gccgcgcgcg gcggggtgcc
     8761 ggtgtctggg tgatccagca tctgccgaat caccgggtcg gtctcgattg tcgtcagcgt
     8821 cacggttggc tccatcgcaa ttccagcatg tcgatgagga gagtcacgaa cgttggaaga
     8881 ggttccgctc cgccgcgagc cgctcggcga cccattggcg cgacgattcg agcgcctcgc
     8941 gctgcaatcc acgggttcgc tcgacctgcg cgggggaggt gccaccgagg ctatcgcggc
     9001 ctgcaacgct tgtccatgca tccaccggcg gccgtgcctc agagaacacc gggtggatct
     9061 ccgtccactc ctcgtcagtc agatcggcaa atgtcttgcc atgggagacg cacttcccaa
     9121 ccagcgagcc gaccacgtgg tgtgcctcgc ggaacggcac gccatgtttc gcaagtaggt
     9181 cagcggcatc cgtcgcgagg gtgaagtcgg caactgcggc tgtggccatt cgctccttgt
     9241 cgaaggtggc gctttgcatc atcggcggga agacctcgag gatcgccagc accgtgtcga
     9301 tgctgtcgaa gagcggctcc ttgtcctcct gcatgtcctt gttgaatgcc agcggcaacc
     9361 ccttcgtgat ggtcagcagg gccatcagat tgccgtagag gcggccgctc ttgccccggc
     9421 ccagctcggc aatgtcggca ttcttcttct gcggcatgat cgagctaccc gtcgagaagc
     9481 ggtcggccag ctccatgaag cggaactcgc ctgacgtcca gaggatgact tcctcggaga
     9541 gccgggagac gtgcatgccg atcacggcag acgcagacag ggtgtcgatc acgaagtcgc
     9601 gatcggagac ggcgtccagg ctgttgatgc tcacctcggc gaagccgaga tccttcgcga
     9661 ccatctcacg gtcgatcggg taggtcgtcc ctgcgagagc ggccgatccg agtgccagga
     9721 cattcgtacg tgcccacgcc tgccggatgc gctcgatgtc ccgttggaac atggcgacat
     9781 aggcctgcat gtggtgggcg acgagcacgg gctgagcacg ctggaggtgg gtgtaccccg
     9841 gcatgatggc gtcgcgatac cgctcgccga gatcgagcag cgcgtcgcag aggttgttga
     9901 gaccatcgac gatcgaggcg agccgctggc gagtccagaa acggacgtcg ttgacgacct
     9961 ggtcgttccg gctgcgcccg gtgtgcagct tgccggcggt ctggccgacc aactcgcgca
    10021 ggcgtcgttc gaccccggtg tgcacatcct cgtcactgat ggtgaggacg aactctcccg
    10081 cctccacctc atcgaggatg cgccagaggc cgagctccag cgcctcggcg tcgtccaccg
    10141 ggatgatgcc ctgcgcgccg agcattctcg catgcgcgat cgacccccga atgtcctcgc
    10201 tgacaagacg aatatcgaac gtcaccgacg cattgaaggc ttcgagccgg gcgtccggca
    10261 cctccatgaa ccggcctccc cacgctttcg cgcgaacgtc accagcgccg ccgttgtccg
    10321 tcatgtggtc tcactcccaa ggtgtgatca tggctggacg ctcgagtgag tcacccgatg
    10381 tgccacgcgc aatgcaagga agagtgcaac agttttcgcc tcgagccgcc tgtgctctgg
    10441 cgaaacgctc cggaatactg gcgtatcgtg tgtgaacaca agatacgata ctaataaacg
    10501 gataggttgt aatgccaatg ttacaaccac tatgcatctt cagggtccgt tttcgtctcg
    10561 aagtaggcgc gcatcgtttc gatgagcttc tcttcgctgg cgtgagtgta gagcatggtc
    10621 gtgtcgatat tcttgtgtcc gaggagcgcc atggcgatcg gatggggaat gttgtcgagc
    10681 atgagccggc tcgtggcggt atgccggaag gagtgaaaca ccgcgtcggg aatccctgcg
    10741 gcctcacggc gctgccgaaa gcggttgccg acattggttg gcgtgagtgg cgtgcggacg
    10801 cgagagggaa acacgaggtc atgctcggtc caggtcgttg ccgcgtcgcg cagctgatcg
    10861 acgtaggcct gctgattccg cagcgcctct gccgaggcaa gcggaaaggg tacgatccgc
    10921 acaccggcct tcgtcttcgg cgtggtgaac tgcagcgcac ctgcgatctt gttcccggca
    10981 tcgtcgtatt cagctcgtga tccggtgagg ttgcgggtta ccatcaggat gccgcgctcg
    11041 aggttgatgt cgccccactg cagtgccagg agttcgcctt gccgcaggcc ggtgtcgatc
    11101 gccagtcggt acaggctccg
//
LOCUS       DASYWL010000336         2678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_242131, whole genome shotgun sequence.
ACCESSION   DASYWL010000336 DASYWL010000000
VERSION     DASYWL010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242131"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..404
                     /locus_tag="VGR22_11800"
     CDS             198..404
                     /locus_tag="VGR22_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129292.1"
                     /translation="MFPGLNVSDVEAAQSCQIDLHTMAHHRPSAPIVTVPLPPWPHQI
                     GRLARMLAGMLAFLHLRHSSRPVA"
     gene            888..1115
                     /locus_tag="VGR22_11805"
     CDS             888..1115
                     /locus_tag="VGR22_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129293.1"
                     /translation="MTTLLIDALLVFWLLLFGAMALLPVLTGTTSARRQPDRPTEDIV
                     ISIAPARPATQPQGQRTPLLPRDEQRDRPAA"
     gene            1249..1941
                     /locus_tag="VGR22_11810"
     CDS             1249..1941
                     /locus_tag="VGR22_11810"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEV2129294.1"
                     /translation="MCGRYVIEDYQELSERLSQVPFRYDFELPPNWNAAPTQTLPVVV
                     EEDDAWVVKGMHWGLIPKWTKPGQRPKVMPINARSETVAEKPMFKPLISQRRCLVPAN
                     GFYEWQQRAGGGGKQPWFIHPKDHDLFLFAGLWDEANAGPDGEPIQSFTILTTDANAD
                     VSALHDRMPVALDDDVMPDWLDREETEVEPLVHLLSPAPDGSVEMHEVSRAVNSPQNN
                     TPELVAPLDGEE"
     gene            complement(2172..2507)
                     /locus_tag="VGR22_11815"
     CDS             complement(2172..2507)
                     /locus_tag="VGR22_11815"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV2129295.1"
                     /translation="MAIALSVSWQTSLIVFGAFMAYQQFENYVLLPRVYGNTLQVTSL
                     SILVGVLVGGQLLGILGIVLSLPLTASIPVIERVWREQIPEDIEELEREAPVTREPTP
                     TELVRSERA"
     gene            complement(2486..>2678)
                     /locus_tag="VGR22_11820"
     CDS             complement(2486..>2678)
                     /locus_tag="VGR22_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129296.1"
                     /translation="VVSGYVIGQMIISAMFGTFSYVVLFVLDVPQPLLLSLFGAVAVA
                     IRSSACRLQRSRRWRSRSP"
BASE COUNT          507 a          940 c          818 g          413 t
ORIGIN      
        1 acaggaatcc gcccaacacc gaaacacgaa tacgtacatc taccgaggcg gcagcggggc
       61 cggaagcgca tcctgcaggt gggcccagca ggcatgctcg ctcagctcac cggcaccggg
      121 aacggtcgat atcaccatgg attcccggcc gcgcgctgca acgaagacca tcacccccgc
      181 atcaaggagc gaccgccatg tttccaggac tgaacgtttc cgatgtcgaa gcagcgcaga
      241 gctgtcagat tgatctccac accatggcac atcatcggcc gtccgccccg atcgtgaccg
      301 ttcccctgcc accctggccg caccagatcg gtcgcttggc ccgcatgctg gcaggcatgc
      361 tcgccttcct gcacttgcgc cactcgtcga gaccggttgc ctgacccaca cggccaggca
      421 tgatcggggc gatactccgg agaacacccg cgcctttccc cggcatgggc gcaacggcaa
      481 cacccgcttc ctgagccccg tcccagcggt ctgggaggca tgcccgccgg gtcgtccttg
      541 cgccatcgcc catcagggcc gcgaacgtac gcgaacattc gtcccagaaa tgtcttgaca
      601 gccgggcacc gacatgagaa catgcgttcg tatcgatacc gctctgcccc gacggagcga
      661 gtcgcggacg gctcaagttt gacaccgtgg caccgtatgt gcgtacgtac agtgcataag
      721 tggttcagcc gggtggctcg gccccgagtc aatcccgccc cggttccccg caccagcatc
      781 ggccgagatc gctcggccgc ggatttgccg gcgctgtgcc gaagcgcagc ccgtgacgga
      841 tcaaacgttg atgcatcccg gccaggccgg gggaaaggaa ctgagacatg acgactctgt
      901 tgatcgacgc cctgctcgtc ttctggctgc tgctcttcgg tgccatggcc ctcctgccgg
      961 tgctcaccgg caccaccagt gcccggcgcc aaccggaccg cccgaccgaa gatatcgtta
     1021 tcagcatcgc gcccgcccgt ccggcgaccc agccgcaggg gcagcgcacc ccgctcctgc
     1081 cgcgcgacga gcagcgggac cggccagcgg cgtaacgttg gatgaagcac ccgcaccatt
     1141 cccaatcccc tcccccttcc ggacctgggc tggatgcgta cgcgcctccg gcccgggtat
     1201 cgtttcacca gcggaccgat tgattcgcgc tcggaaagga tcacgaccat gtgcggacgc
     1261 tacgtcatcg aggactacca ggagctcagc gagcggctgt cgcaggtgcc cttccgctac
     1321 gacttcgagc tgcctcccaa ctggaatgcc gccccgaccc agaccctgcc cgtcgtggtc
     1381 gaggaggacg acgcctgggt cgtgaaggga atgcactggg ggctgatccc gaagtggacc
     1441 aagcccgggc aacgcccgaa ggtgatgccg atcaacgccc gcagcgagac ggtcgccgag
     1501 aagccgatgt tcaagccgct catcagccag cgacgctgtc tcgtacccgc caacggtttc
     1561 tacgagtggc agcagcgcgc gggtggtggc ggcaagcagc cgtggttcat ccacccaaag
     1621 gaccacgatc tcttcctctt cgccggcttg tgggacgaag ccaacgccgg gccggatggc
     1681 gagccgatcc agagcttcac cattctcacg accgacgcga acgcggacgt cagcgccctt
     1741 cacgaccgca tgccagtcgc gctcgacgac gatgtcatgc cggactggct cgaccgcgag
     1801 gagaccgagg tggagccgct ggtccacctg ctctccccgg cccccgacgg atcggtggag
     1861 atgcacgagg tgagccgagc ggtcaactcg ccacagaaca acacgccgga gctggtggct
     1921 cccctcgacg gcgaggagtg acgcctggca agcgtcaccg cgaagccctc cctgccgcgt
     1981 cgatacgcgg aatccccgag atcatctcac gcggtagacc cgccctgcaa atgggagggt
     2041 tcgatcatga gcggcgccgc gtggttgcat gacgctcatc ttcagggcta cgcaacgaga
     2101 ctcgcgcgta gagacccagg cagatctgct ggcaggtggc tgcctcgtcg ctcgcatctg
     2161 ggtggtcgcc gtcaggcgcg ctcacttctg accagctcag tcggggtcgg ctcgcgcgtg
     2221 acgggggcct cccgctccag ctcctcgata tcttccggga tctgctcgcg ccagacacgc
     2281 tcgatcacgg ggatcgacgc tgtcagcggc agcgatagca cgatgccgag gatccccagc
     2341 agttgcccgc cgacgagcac accgacgagg atgctcagcg aggtcacctg cagtgtgttt
     2401 ccgtagacgc gcggaagcag cacgtagttc tcgaactgct gataggccat gaacgccccg
     2461 aacacgatca gcgaggtctg ccagctcacg gagagcgcga tcgccatcgc cgcgatcgtt
     2521 gcaaccggca cgccgatgat cggatcgcca ccgccacggc gccgaagagc gagagcagca
     2581 gcggctgcgg cacatcgagg acgaagagca caacgtagga gaacgtgccg aacattgccg
     2641 agatgatcat ctggccgatc acgtacccgc tcaccaca
//
LOCUS       DASYWL010000337         7556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_298785, whole genome shotgun sequence.
ACCESSION   DASYWL010000337 DASYWL010000000
VERSION     DASYWL010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7556
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298785"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(239..403)
                     /locus_tag="VGR22_11825"
     CDS             complement(239..403)
                     /locus_tag="VGR22_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129297.1"
                     /translation="MNFIDAMGLLVALLSEARRVEISTAADGQALDYIQHLGRRDGAV
                     AMEHQAWGVA"
     gene            323..883
                     /locus_tag="VGR22_11830"
                     /pseudo
     CDS             323..883
                     /locus_tag="VGR22_11830"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26.; internal
                     stop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
     gene            complement(966..3578)
                     /gene="lon"
                     /locus_tag="VGR22_11835"
     CDS             complement(966..3578)
                     /gene="lon"
                     /locus_tag="VGR22_11835"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEV2129298.1"
                     /translation="MTNEELKNQQHAEQETPDEERTEEQTLKADVTQSTPDPQAEHRP
                     SGDDAGGTADMAENEELLLPLLPLRGTVVFPQTLVPLAAALPRSLRLIDDVMSDDRTV
                     AMVLQKDAENEHAGPEDILEIGTIATIHQMMRVPDGTVRLAVQGRERMRIVEVVQEEP
                     FLKARVIRIPEPPEDSVEVEALSRNLVDLFQRLVGLVPHLPEELITAAINIDDDPRHL
                     VYLVASNLRMEAEERQKLLEIDSISQKLRELNAFITKELDVLELGRKIQTDVQDEMSK
                     SQREYFLREQMKAIQRELGEESETETEVNELREKIESSGMPEEAEREARRELDRLSKL
                     PPAAAEYGVIKTYLDWLTALPWNVTTENEIDIAKARQILDEDHYGLDRIKDRILEYLA
                     VRKLKQDQATEEGEPLVHREPILCFVGAPGVGKTSLAQSIARALDREMTRMSLGGVRD
                     ESEIRGHRRTYVGAMPGRIIQAIRRAGSKDPVFVLDEVDKLGSDWRGDPSSALLEVLD
                     PEQNDSFRDHYLDVAFDLSQVMFIATANFLDQIPAPLRDRMEILELSGYTDEEKLNIA
                     RRYLVPKQMKANGLRSVEVEWSDEALMAVIQHYTREAGVRNLEREIGTVARKLATALA
                     SEKEIPQRIGKAEIREYLGRPRFFYEERAARTALPGVSIGVGVTGVGGDIMFIEATRM
                     AGKGSMTITGQLGDVMKESASAAMAFVRSRASDLGLDPRFYETADIHLHVPAGATPKD
                     GPSAGTAMTTALTSLLSGVPVRDDVAMTGEITLRGQVLPVGGIKQKALAAQRAGITTF
                     ILPKRNEVDLDDLPEELQEGMTFVLAETMDDVLKAAMPDEFFDRTVNFDTGGAPETHE
                     ANEPVDRQAASSVA"
     gene            complement(3575..4051)
                     /locus_tag="VGR22_11840"
     CDS             complement(3575..4051)
                     /locus_tag="VGR22_11840"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEV2129299.1"
                     /translation="MIVIRRGRPRPATRKSNEVDEVVRALMSGQRSVGMRSFQTWRPM
                     LDVYSTGDAFEVVAELAGMRGDDIDVLIEGDVLVISGVRERPAADNCLSYYEARIPFG
                     PFRAEVVVPFEIDWEATTADYTNGFLTVSLPRRQPHAVPVRRRDSADEAEESEIDS"
     gene            complement(4164..5114)
                     /locus_tag="VGR22_11845"
     CDS             complement(4164..5114)
                     /locus_tag="VGR22_11845"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HEV2129300.1"
                     /translation="MIGTRNVSSTLSVAISTGIALLRLARPQQWVKNAVVLAGMVFAA
                     LSDRPSAIVDALVALLAFIAASSAIYVANDLRDMEADRHHPLKRTRPLATGAVQPRTA
                     AWFAVGLVVLSLAVSVLLTPVLALTVAIYLLIMTGYNRWFKQVPILDVTVIAFGFVLR
                     AVGGAVAVGVPISPWLLLCAFLLALFLGFGKRRAELIELGDGATRHRQSLGGYTAPLL
                     DQLVAITAVSALVTYAVYTVVSRSVPANDLMLFTVPFVAFAIFRYLFLVYGRGLGGSP
                     ESLLFRDGWLLATVALWGIAALVIVQTHGDPSLVLVGNDS"
     gene            5642..6628
                     /locus_tag="VGR22_11850"
     CDS             5642..6628
                     /locus_tag="VGR22_11850"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV2129301.1"
                     /translation="MRVETSNIFDVMREDLDLVRDRVADAARVDFPIVSGMVRDIVEA
                     GGKQLRPIVLLLAGKAFTYDRDRLITAAAGVELLHTASLVHDDSIDRASIRRGQPTLN
                     SRINTGAVILIGDFLFAQSAILAAATQNPRVVAIFASSLGDICDGQLREMLSAHNVRQ
                     ERDEYLKRIYGKTASLFAGAAEMGAVIGGASEADIEQMKGYGADLGMAFQIIDDVLDL
                     REQSQAIGKPAGNDLRQGVVTLPTMYYLEGLDEGSDEFEIVERVIAGEETRDAGVNGL
                     VDAIRASGALEMAIQMADGYIASARARLDVVRNPDVRRHFEEILDIAVSRTA"
     gene            complement(6699..>7556)
                     /locus_tag="VGR22_11855"
     CDS             complement(6699..>7556)
                     /locus_tag="VGR22_11855"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEV2129302.1"
                     /translation="RLVGVSTVAGNVPDHVATANTLSVLAWLGAEPVPVHRGASRPLA
                     LPYYDAVQVHGGNGLGGAELGGSAAKEARTNGVQAILDAAEAHDGLLVLAALGPLTNL
                     AMALSIRPGLVDQVQKLTLMCGAFTIPGNVTQHAEYNAFADPDAASQVMGARWNELLV
                     VGLDVTHQTIFARDQWDALEEDGSASAELVRRITERTFKERGVDALYLHDPLAVAVAL
                     DDSLLRTEQQAVSVNTAGDDHRGKTTLAGNGAARIALQVDAERFERWCAELLDLPASG
                     KREPRDRIE"
BASE COUNT         1355 a         2577 c         2281 g         1343 t
ORIGIN      
        1 cctgtcaagc ccgcctcggt gaccgccgtg cgatccccga gccgcgcccc gccaggcgag
       61 ccgccctctt caggcgcgac gaccagtgta ccaccgctcg cctcgccccg tcaagggcct
      121 gtgtcgcatc cgttcgcggg atgccgagcg tctcggcccc gcacccggtc ggacaccgtc
      181 ggcactcggg ctcttgtgcc cgagcgccca gcaactctag ccgcgctcca ccccgatgtc
      241 aagcaacgcc ccacgcctga tgctccatcg ctaccgcacc gtcacgccgg ccgagatgct
      301 gaatgtaatc gagggcctgg ccatcagcgg cggtggagat ctcgacccgg cgcgcttcgg
      361 acaacagcgc cacgaggaga cccatggcat cgatgaagtt catgacccat tcgagtttgc
      421 gctcatttag gtcgctgccg agcgcgatgt gtcaatgctc gccgtctgtc gaagcatcca
      481 ggtaatgtcc gtggcgttcg gcggctcgcc ccaccagcat gtcagcgagg tggtctccgg
      541 cgagttcgaa catcgccagc acaaggccgg tttcacctag catcagcaat cacacctggt
      601 tgtactggag aacacgccga acgcggtgag ttcactgctc ggcaagacag agctgatggt
      661 gaactctttc caccgtcagg ctctcacccg cgtcccggag ccactccgcc tcgccgggcg
      721 ggccgatgac ggaacaatcg aggccatctg gcacccggag atgtcgttcg gcatcggcgt
      781 gcagtggcac ccggagacgc tggcaccgga gtacgaaaac gatgcccggc tcttctcggc
      841 atttgtcccg gccgccacga agaagtcggc cattacgacc tgacagccat caagaggcag
      901 acaccgaacc ggcgccatgc ttaagctgag cgtggcgccg gttggattgc gtctcctact
      961 ggtgctcagg cgacgctgct tgccgcctgg cgatcgactg gctcgttagc ctcatgggtt
     1021 tcgggagcgc cgcccgtgtc gaagttcacc gtgcgatcga agaactcgtc cggcatggct
     1081 gccttgagca catcgtccat cgtctcggcg agcacgaagg tcatgccctc ctgcagctcc
     1141 tcgggcaggt catcgaggtc gacctcgttc cgcttgggca ggatgaaggt cgtgatgccc
     1201 gcacgctgtg ccgccagcgc cttctgcttg atgccaccga cgggcagcac ctgtccgcgg
     1261 agcgtgattt cgccggtcat cgcgacgtcg tcacgcaccg gcacaccgga gagcagcgag
     1321 gtgagcgccg tcgtcattgc cgtaccggcc gaaggcccgt ccttcggcgt cgcaccggca
     1381 ggtacatgca ggtggatgtc cgccgtctcg tagaaccgag gatcgagacc gaggtccgat
     1441 gcgcgggatc gcacgaacgc catcgccgcc gatgccgact ccttcatcac gtcgcccagc
     1501 tggcccgtga tcgtcattga gcccttgccc gccatgcggg tggcctcaat gaacatgatg
     1561 tcgccaccga caccagtcac gccgactccg atcgacacac ccggcagcgc cgtgcgcgcc
     1621 gctcgctcct catagaagaa tcgcggccgg cccaggtact cacggatttc cgccttgccg
     1681 atgcgctgcg ggatctcctt ctccgaggcc agggccgtgg caagcttgcg ggcgacggtg
     1741 ccgatctcgc gctcgaggtt gcggacaccc gcctcgcgag tgtagtgctg gatgaccgcc
     1801 atcagcgcct cgtccgacca ctcgacctca acgctcctca ggccattcgc cttcatttgc
     1861 ttcggaacga ggtaccggcg ggcgatgttc agcttctcct catcggtgta tccactgagc
     1921 tcgaggatct ccatccggtc gcgcagcgga gccgggatct gatccaggaa gttcgcggtg
     1981 gcgatgaaca tcacctgtga aaggtcgaag gcaacatcga ggtagtgatc ccggaacgag
     2041 tcgttctgct cgggatcgag cacctcaagc agagccgagg acgggtcacc gcgccagtcg
     2101 ctgccgagct tgtcgacctc atccagcacg aacaccgggt ccttcgagcc cgcccggcgg
     2161 attgcctgga tgatccggcc gggcatcgca ccgacgtagg tgcgacggtg accgcggatc
     2221 tccgactcgt cgcgtacacc accgagcgac atgcgcgtca tctcgcgatc caacgctcga
     2281 gcaatggact gcgcgaggct cgtcttgcca acgccgggcg cacccacaaa gcagagaatc
     2341 ggctcccggt ggacgagcgg ctcgccctcc tcagtggcct gatcctgctt gagcttgcgc
     2401 acagcgaggt actccaggat gcggtccttg atccgatcga ggccgtagtg gtcctcgtcc
     2461 aggatctgcc gcgccttggc gatgtcgatc tcgttttccg tagtgacgtt ccacggcagc
     2521 gccgtcagcc agtcgagata ggtcttgatc acgccgtact cggcggctgc aggtggcagc
     2581 ttggagagac gatccagctc gcgccgggcc tcgcgctccg cttcctccgg catgccggac
     2641 gattcgatct tctcacgaag ctcgttgacc tctgtttcag tctcgctttc ctcaccaagt
     2701 tcacgctgga tggccttcat ctgctcgcgc aggaagtact cgcgctgcga cttcgacatc
     2761 tcgtcctgaa cgtcggtctg gatcttccgg cccagctcga ggacgtcgag ctccttggtg
     2821 atgaacgcgt tgagctcgcg caacttctgc gaaatcgagt cgatctccag cagcttctgg
     2881 cgctcctcag cctccatccg caggttggat gcgacgaggt agacgagatg ccgcgggtcg
     2941 tcatcgatat tgatcgccgc cgtgatcaac tcctcgggca ggtgcggaac gagcccgacc
     3001 agccgctgga acaggtcaac gaggttgcgc gagagcgcct cgacctcgac cgagtcctct
     3061 ggcggctcgg gaatccgaat cacccgcgcc ttgaggaacg gctcctcctg cacgacctcg
     3121 acgatcctca tccgctcgcg accctgaact gccaggcgca ccgtaccatc cggcacgcgc
     3181 atcatttggt ggatcgtggc gatcgttccg atctcgagga tgtcttctgg gcccgcgtgt
     3241 tcgttttcgg cgtccttctg cagcaccatc gcaaccgtgc gatcgtcgga catcacatca
     3301 tcgatcaggc gcagcgaccg cggcagcgcc gcagccagcg gaaccagcgt ctgtggaaag
     3361 acgaccgttc cacgcagcgg cagaaggggg agcagcagct cctcgttctc cgccatgtca
     3421 gcagtgcccc cagcgtcatc gccagaaggc cgatgctccg cctgggggtc aggcgtggat
     3481 tgggtaacgt ctgccttcag cgtctgttcc tcggttcgct cttcgtccgg ggtctcttgc
     3541 tcagcatgct gctgattctt cagctcttca ttcgtcatga atcgatctca ctctcttccg
     3601 cctcatcagc cgaatctcgg cggcgcacag gaacggcgtg cggctggcgt cgcggcagcg
     3661 agacagtcag gaacccgttg gtgtaatcgg cagtcgtcgc ctcccagtcg atctcgaagg
     3721 gcaccacaac ctcggcccgg aagggcccga aagggatgcg ggcctcgtag tacgagaggc
     3781 agttatcggc agccggccgt tcgcgaacac cgctgatcac caggacatcg ccctcgatca
     3841 gaacatcgat gtcgtcgccg cgcatgcccg ccaactcagc caccacctcg aacgcgtctc
     3901 cggtggagta cacgtcgagc atgggccgcc aggtctgaaa cgagcgcatc cccaccgacc
     3961 gttggccgga catgagcgcc cgcacgacct cgtctacttc gttggacttt cgcgtcgccg
     4021 gacgcggtcg ccctcggcga atcacgatca tgcttcctcc acgtttctct cggatgcgaa
     4081 acaggcgtca atgcccgagc atgccgccgt ctcacgactg cacgaattat aactctcgaa
     4141 gcaggtaatt gctacgattc gtatcaacta tcgttaccaa cgagaaccag cgaagggtcg
     4201 ccgtgagtct gcacaatcac cagcgcggcg atcccccaca gcgcgactgt cgcaaggagc
     4261 cagccatcgc gaaacaggag gctctcaggg ctgccaccca gaccccgccc gtacacgagg
     4321 aacaggtacc ggaagatggc aaaggctaca aagggcacag tgaagagcat caaatcgttc
     4381 gccgggacac tcctggatac caccgtgtat acggcgtagg tgaccagtgc gctgactgcc
     4441 gtgatggcaa caagttggtc gaggagcggg gccgtgtagc cgccaagcga ctggcgatgc
     4501 cgggtggcgc catccccaag ctcgatcagc tccgcccgcc gcttgccaaa gccgaggaag
     4561 agcgccagca ggaaggcgca gagcagcagc cacggcgaaa tcggcacgcc gaccgccact
     4621 gcaccaccca cagcgcgcag gacgaaaccg aacgcgatca ctgtcacgtc gaggatcggc
     4681 acctgcttga accaccggtt gtagccggtc atgatcagca ggtatatcgc gaccgtcagc
     4741 gccagcaccg gtgtcagcag cacggataca gccagcgaga gcacgacgag acccaccgcg
     4801 aaccacgcgg cggttcttgg ctggacagcg ccggttgcca gtgggcgcgt ccgtttgagg
     4861 gggtgatgcc gatctgcttc catgtcccgg aggtcgttgg cgacgtagat cgcactgctg
     4921 gcggcgatga atgcgagcag cgcgactaaa gcgtcgacga ttgcgcttgg gcggtcgctg
     4981 agagcggcaa agaccatccc cgccagcacc accgcgttct tgacccactg ctgagggcgc
     5041 gccagacgca gcagcgcgat gcctgtcgag atcgcaacac ttagggttga gctcacattc
     5101 cgagtgccga tcatgctcga cgcctttcca cacgtatcgg tcaacatgca tctgcgaatt
     5161 gcgtcggcct caggctcgaa cgcatcactc cagagcatcc agtcgctcgc ctcaacccct
     5221 ccgccacgcc tgtccggtcg tggcgaacgt ctgcgtcggc gcgcgcgagc ccgcatcggg
     5281 caaggtcctg cgtccccggc cgcaacctga cctcgagcat cgaaccagca ggccgaccgg
     5341 tgcccggttc ctgtagatcc tctcataggg taaccgttct ggcggccgga gcctgattcg
     5401 agctgcttcc tccagagcgt gatgcgccgg caaccgccag gcgcttgtcc tctcgagccg
     5461 ccggtggcgc cgttggtccc atcccaccgc agcaatgcgg cgaagttgcc agaagggagg
     5521 ccagcacagg tacctgcagt acggggactg tttgacaccg ttgtttctat ccgcggataa
     5581 tcacgaccag catgcccgct cttcccgtga agacgggaag ggcctaccag cgagggcgcc
     5641 agtgagggtc gagacatcga acatattcga cgtcatgcgc gaggatctcg acctcgttcg
     5701 cgaccgtgtc gccgatgccg ctcgtgttga cttcccgata gtctccggca tggtgcgtga
     5761 catcgtcgag gccggcggca agcagctgag accaatcgtg ctcctgctcg ccggcaaggc
     5821 cttcacctac gaccgtgatc ggctgatcac cgctgcagcc ggggtggaac tgctgcacac
     5881 ggcctcgctg gtccacgacg acagcatcga ccgagcctcc atccgccgcg gccagccgac
     5941 tctgaactcc cgaataaaca caggcgcggt catcctgatc ggcgactttc tcttcgcgca
     6001 aagcgctatc ctcgccgccg caacgcagaa cccacgcgtc gtggccatct tcgcctcctc
     6061 cctcggcgac atctgcgacg ggcaactgcg ggaaatgctg agtgcgcaca acgttcggca
     6121 ggaacgggat gagtacctca agcgaatcta cggcaagacc gcgtccctct ttgccggtgc
     6181 tgccgagatg ggcgctgtta tcggtggcgc ctccgaggcc gacatcgagc agatgaaggg
     6241 ctacggcgcg gacctcggta tggccttcca gatcatcgac gacgtccttg accttcggga
     6301 acagtcccag gccatcggca agccagcggg caacgacctc cgccagggcg tggtcactct
     6361 cccgacgatg tactacctcg aggggctcga cgagggttcg gacgaattcg agatagtcga
     6421 acgggtcatc gccggcgagg aaacacgtga cgccggagtg aacggccttg tcgatgccat
     6481 cagggcatcc ggagcgctcg aaatggcgat ccaaatggcc gacggctata tcgcctcggc
     6541 acgagcgcgc ctcgatgtgg tccgcaaccc cgacgttcgc cggcacttcg aagagatcct
     6601 cgacatcgcc gtctcccgta ctgcctgaag cgcgccgcag cctcgcggaa gcgcgccact
     6661 gcagattcga cctcgggcga gcacgtgtct cgttgcgact actcgatgcg atcccgtggc
     6721 tccctcttgc cggacgccgg cagatccaga agctccgcgc accatcgctc gaatcgctcc
     6781 gcgtccactt gcagtgcgat cctggccgcc ccgttaccgg cgagcgtcgt cttgccacgg
     6841 tggtcgtcgc cggcagtatt cacgctgacc gcttgctgct ccgtcctcag cagcgaatcg
     6901 tcgagtgcta cggcgacggc cagcgggtca tgcaggtaga gcgcgtcgac gcctcgctcc
     6961 ttgaaggttc gttccgtgat ccggcggacc agttcggccg atgccgatcc gtcctcttcc
     7021 aacgcgtccc actgatctcg cgcaaagatc gtctgatgcg tgacgtcgag cccgacgaca
     7081 aggagttcgt tccagcgggc acccattacc tggctggccg catccggatc ggcgaacgcg
     7141 ttgtactcgg cgtgctgtgt gacgttccct ggaattgtaa aggcaccgca catgagggtg
     7201 agcttctgga cctggtctac gagacccgga cgaatgctta gcgccatggc gaggttggtc
     7261 aggggaccca acgcagccag gaccagcagc ccgtcgtgcg cctctgcggc gtccaggatc
     7321 gcctgaacgc cgttggtccg cgcctccttg gccgcgcttc cgccgagttc agcacctccg
     7381 agcccgttcc cgccgtgtac ctgcacggcg tcgtagtacg gcagcgccag cgggcggctg
     7441 gccccgcggt gtaccggcac cggctccgcg cccagccagg caagcacgct cagcgtgttc
     7501 gccgtcgcca cgtggtccgg cacattcccg gcaaccgtcg agaccccgac cagcct
//
LOCUS       DASYWL010000338         2578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_430239, whole genome shotgun sequence.
ACCESSION   DASYWL010000338 DASYWL010000000
VERSION     DASYWL010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430239"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(346..480)
                     /locus_tag="VGR22_11860"
                     /pseudo
     CDS             complement(346..480)
                     /locus_tag="VGR22_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(567..1778)
                     /locus_tag="VGR22_11865"
     CDS             complement(567..1778)
                     /locus_tag="VGR22_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129303.1"
                     /translation="MNHSTEQRAASALPASVNSLPRPDDPAQSSFRSFWMGGFEAAGH
                     INESGARVNMLAATQHDRYVAEDFALVRSVGIGTVRESAQWHLIDRDGALDFSTLAPV
                     AEAAQAAGVQVLWTLCHYGWPEDLDLFSDELVARFAAYCEAAARFLASYSDDPPLYAP
                     MNELSFFSWACGDEGLFHPYVRDRGVELKRHLARAVIAGCEAILRADSRARLCHIEPI
                     INVFPPLDRPDLVEEAAAFHELQFAAWDLLAGRQNPEIGGHPRYLDLIGVNFYYDNQW
                     EYLGKRLRWTDVPRDARWVPVRELLANVWRRYRRPLFVAETSNFGAGRVPWLREMAAE
                     VRAAIDLGVPVQGVCLYPIIDRPSWHDPDHWHNSGLWDLIPNETGRLERVLYEEYAAE
                     LVAARVLLPDQ"
     gene            complement(2224..>2578)
                     /locus_tag="VGR22_11870"
     CDS             complement(2224..>2578)
                     /locus_tag="VGR22_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129304.1"
                     /translation="SWEQEAVLAATSDDTVQTRLYDLIGERPFPAENPDRMLRNAFID
                     RWTNRESEIPAQREALQAEVAAGHEHADLAVAGVSAGVSAGLIASARPAGEIVRDIVQ
                     EAEDLLRERPRKLLG"
BASE COUNT          473 a          828 c          846 g          431 t
ORIGIN      
        1 cagagtaagt aacaccataa tgagtgacac tgccgcgttg gtgtccacca accgagagaa
       61 aataaacatt tcggtgcttg tcttggcgac aggacatggg aggtaagcaa caaggcctca
      121 caccccacca caggcgtggt acaggactcc cttgcacacc gttccgcgct cttggcgaga
      181 cgtagcagga gttcgttagt tcacagcacc atcatctcgg gcgcagtacg cagaggcgct
      241 gctggtgaat ctctatcagc actacgacca gaatcagcca tgcagcgcgt gcgcattccc
      301 gatctcactc cacggaactt ctttgcttgc acttagaggc tggtttcaaa ccggcagcca
      361 gggctggatc gccgcgaggg tgatgagcgc atgataggtc tcgtccagtt cttcgtaccg
      421 ggtggcaatt gcccgatgct gcttgagtcg gttgatcgtg tgctcgatcc ggttgcgttc
      481 aacgcgtcca tccccctagg gtggtgagcg cgcggtcagg aagccgggtc aacggggcag
      541 ttccgtccgt ccatccggct ctccgcctac tgatccggta gcagcacgcg agcagcgacc
      601 agctcagccg cgtactcctc atagaggaca cgctcgagac gaccggtctc gtttgggatc
      661 agatcccaaa gaccgctgtt gtgccagtgg tcagggtcat gccagctggg gcggtcgatg
      721 atgggataga ggcagacgcc ctgcaccggc acgccgaggt cgatcgctgc ccggacctcg
      781 gccgccatct cccgcagcca aggcacacgg cccgcgccga aattgctcgt ctcggcgacg
      841 aagaggggac gccgataccg tcgccataca ttggccagca actcgcgtac cggcacccag
      901 cgagcatcgc gcggcacgtc ggtccaacgg agccgtttgc cgagatactc ccactggttg
      961 tcgtagtaga agttgacacc gatcaggtcg aggtagcgtg ggtggccgcc gatctccgga
     1021 ttctggcggc cggcgagaag atcccaggcg gcgaactgga gctcgtggaa ggcagccgcc
     1081 tcctcgacca agtccggtct gtccagcggc gggaagacgt tgatgatcgg ctcaatgtgg
     1141 cagagacgcg cacgcgagtc ggcacgcagg atggcctcgc agccggcgat gaccgcccgc
     1201 gcgaggtggc gcttgagttc cacgccacgg tcacgcacat aaggatggaa gagcccttcg
     1261 tcgccacatg cccagctgaa gaaggagagc tcgttcatcg gcgcgtagag cggcgggtcg
     1321 tcgctgtagg acgccaggaa gcgcgccgcc gcctcgcagt aggcggcgaa gcgggccacc
     1381 agctcatcag agaagaggtc gagatcctcc ggccaaccat agtggcagag cgtccaaagc
     1441 acttggacgc ccgccgcctg ggcggcctcc gcgactggcg cgagcgtgga aaagtcgagc
     1501 gcaccatcgc ggtcaatcag gtgccactgg gccgactcgc ggaccgtccc gatgcccacg
     1561 cttcgcacga gggcgaagtc ctcggccaca tagcggtcgt gctgggtagc ggcgagcatg
     1621 ttcacccgcg caccagactc gttgatgtgg ccggcagcct cgaatccacc catccagaag
     1681 gagcggaagg agctttgcgc ggggtcgtcg gggcgcggca aggaattgac gcttgccggc
     1741 aacgcggacg cggcacgctg ttccgtggag tgattcattc gtggtccggt tattccttct
     1801 gtcgatgcac aagctgtgcg gtcaacgcac catcgatggg cggcacaagt gtgcaccgaa
     1861 aggcgcgatc cggatagggc atcgcacgac gccatcaatc ggccacggac ggggtcgaac
     1921 cgttcctgac cctggcctag gtgccggtgc ccaggtgggc cgcgcacggg gcgacgcact
     1981 tgacagtcag cacgatcggg gcaggattcg cctcagtgga cgaacacgtc accgcactgg
     2041 aacgcgccga gacggcgctc aacagttaag tccgtttcac cgtactcgct ggttcggtgg
     2101 cggctccgag gagcgtccgt ccgatggacc gcaggggagc gggcgaccgg gtggcgccgt
     2161 ctggcaaccg cgctgcgtgc cttatccctg ttcattcggc tgggatgggc gaagagggga
     2221 cgatcagccg agcagcttcc gtggccgctc gcgcagcaga tcctcggcct cctgcacgat
     2281 gtcccgcacg atctcgcccg ccggccgcgc cgacgcgatc agtcccgcgg agacgcccgc
     2341 gctcaccccg gccaccgcca gatcagcgtg ctcatgcccc gccgcaactt ccgcttgcag
     2401 cgcctcccgc tgggcgggaa tctcggactc tcgattcgtc cagcggtcga tgaaggcgtt
     2461 gcgcagcatc cggtccgggt tctcggcggg gaaggggcgc tccccgatca ggtcgtagag
     2521 gcgggtctgg acggtgtcgt cggacgtcgc cgccagcacc gcctcctgtt cccaggac
//
LOCUS       DASYWL010000339        17489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_298935, whole genome shotgun sequence.
ACCESSION   DASYWL010000339 DASYWL010000000
VERSION     DASYWL010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 17489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17489
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298935"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1445)
                     /locus_tag="VGR22_11875"
     CDS             complement(<1..1445)
                     /locus_tag="VGR22_11875"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HEV2129305.1"
                     /translation="MAQNIYITSAEGNTGKSTIALGMLETLKRSGKTIGVFRPISRST
                     TGEDYILDLLLAHLWMPDRAPDLIGVSYDDVHADPDAALARIVARYKAIEPEFDVVLI
                     LGSDYTDVSTPTELSYNARIAANLGAPVLLVLGGRSQNELIPSNAQALTGAARSPEEI
                     AQAAEIALAELHDEHAQLIAVIANRVDPEQLAATTGAIFATVSPSGQIPVWSLPEDAV
                     LIAPTLRALMETLDASLYRGDAGLLDREAMSVVVAAMSMENVLVRLTPDSAVVAPGDR
                     SEVLLSLLMAHTSGTFPSLSGIILNGGFDVSPTIERLIEGIGVDLPILRTNLNTFETA
                     YRVSRTRGRFGADSQRKYDRALSIFDAHVDGKAILAVLDVVREDIVTPLMFEYQLLER
                     ARSNRKHIVLPEGDDDRILRAAGTLLERQAAALTILGDENDIRGRASRLGRDLTGACI
                     LSPFDPELRERFAQSYAQLRAHKGVTLEQAR"
     gene            1679..3265
                     /locus_tag="VGR22_11880"
     CDS             1679..3265
                     /locus_tag="VGR22_11880"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEV2129306.1"
                     /translation="MTDVMAEDQLVGGSGERSKAEHLSDFQREMAEQHQAAREKQEAR
                     GKRSALDRVRQLLDDGSFTELDAFVRHRATQFGMAEKRPFGDGVVTGFGTIDGRQVAV
                     FSQDFTVFGGSLGEAFAEKLVKLMELAERCGCPIIGINDSAGARIQEGVEGLAGYGDV
                     FFRNVRLSGVVPQISIIAGPCAGGAVYSPVMTDFTYMVEGVGRMFITGPDVIKTVTGE
                     RVDPEELGGAATHTGTSGVAHFTATDEDDLFDQVRYLLSFLPSNNLDDPPWFAPEDDP
                     DRESEALDSLVPEASNKPYDMHRVLGEVLDDGEFYEVQQNHAQNLIVGFGRLDGHPVS
                     VVANQPSVLAGTLDIDASVKGARFVRFCDAFNLPIITFVDVPGFLPGTDQEYRGIIRH
                     GSKLLYAYCEATVPKLTVIIRKAYGGAYVVMASKHTGADFNVAWPTGEVAVMGPEAAV
                     GLVFRRELAGLEDPDARKKELVQQYEDLFASPYQAASRGYIDDVIEPHTTRPWLIRSL
                     ALARSKRVATPRRKHGNIPL"
     gene            3262..3495
                     /locus_tag="VGR22_11885"
     CDS             3262..3495
                     /locus_tag="VGR22_11885"
                     /inference="COORDINATES: protein motif:HMM:NF025192.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit epsilon"
                     /protein_id="HEV2129307.1"
                     /translation="MSGNAHRDTGILIRVMPEPTPEELAAIVAVLRARRRRQQTMATT
                     EELSHSVSRWARAGREEAMAAWDFGDGKGTGSW"
     gene            complement(3704..4324)
                     /locus_tag="VGR22_11890"
     CDS             complement(3704..4324)
                     /locus_tag="VGR22_11890"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEV2129308.1"
                     /translation="MRTDRITIEDRPEAKLWGIDGPTLDRWPTLVPGREVTVVKHSVM
                     HGPDFRVAYPGTVVTTAVPAPWVEIQAFWVAGTVQQAHLTFEIDDVLREIFSPIHPYN
                     AFAVYSPAGELKGWYANVVYPTVVTASGEQLEVVWNDLFIDIVVTPDGEVAVLDEEEL
                     EEAELLTNDPELHARILVARGELLRRCQARRTPFSSPNQIDVADYR"
     gene            complement(4314..4826)
                     /locus_tag="VGR22_11895"
     CDS             complement(4314..4826)
                     /locus_tag="VGR22_11895"
                     /inference="COORDINATES: protein motif:HMM:NF040503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsinothricin resistance N-acetyltransferase
                     ArsN1 family A"
                     /protein_id="HEV2129309.1"
                     /translation="MSVSTVAPTARAANPTDAEAIARIYNEGIDDRVATFETLHRTAA
                     DIEAWFDGRHPVVVVEIGGEVVGFASTSTYRPRQCYDGIAEYSVYVARSARGRGVGRI
                     AMEALFDAASETGFWKLISRIFPENASSLRLMRSLGFREVGTYRRHAKLDGEWRDVVI
                     VEKLLTDDAD"
     gene            complement(4823..6208)
                     /gene="manB"
                     /locus_tag="VGR22_11900"
     CDS             complement(4823..6208)
                     /gene="manB"
                     /locus_tag="VGR22_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922131.1"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEV2129310.1"
                     /translation="MTDTTQASLEPLDNSVGKLFKAYDVRGIVPDELSPEIAYRIGRG
                     VVAFLDADEVVVGRDMRVSGPAMQAALMDGIRDQGANVVDIGLVSTDTLYFAVGKYGF
                     PAGVMITASHNPANYNGFKVCREDARALSLDTGIGEIRDMVVSGNFPKPRGKRGDLTE
                     KNVLEAYAEHVLSMIDPSRIKPLKVAVDAGNGMGGKLVPAVFDTLPCEIVPLYFELDG
                     TFPNHEANPIEPENVRDLERTVVNEGCDLGIAFDGDADRMFLIDEKGNFIGGDMTTAM
                     VSLELLKHNPGSTIVYNLICSRSVPELIEENGGHAIRSRVGHSYIKAVMREEDAIFGG
                     EHSGHFYFRDNWYADSGVIAALTVLQLISDADQPLSEILDPIDTRVRSGEINSEVEDI
                     EGTVARVEAFYSDKGAEIDHLDGLTVGFDDWWFNLRASNTQPLLRLNVEAETEELLRE
                     KTSEVLELIRQ"
     gene            6344..7342
                     /locus_tag="VGR22_11905"
     CDS             6344..7342
                     /locus_tag="VGR22_11905"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEV2129311.1"
                     /translation="MLDATDIRLRADDPIDLHLHTFQSDGVWTPMQLIDHLAEAGITV
                     AAVCDHETQRSVVEAIEYGERRGIYVIPGVEVTVRHDDRQWHMLIYGIRPDDDRAEAR
                     PFLEMMREHEASLYSLARANWARVERTGRVLPSLETIVGDGKMWTYPEVLVAMIRDGH
                     APNLTIAANMITELGGRFNADIPIEEVVSKAHEAGGVCVMAHPGRPDLGPAMTAETLA
                     KLLADAPVDGLECHYRTYTDRDTAFFRSLAGQHGLVTSAGSDSHGLNTPVNPRPFRAV
                     WAKSLLERLGVVVDDPVDEPVWAPGMDPLAVKPQRKKRGKWKRRQRLGRFWRRKRA"
     gene            complement(7571..9229)
                     /gene="ggt"
                     /locus_tag="VGR22_11910"
     CDS             complement(7571..9229)
                     /gene="ggt"
                     /locus_tag="VGR22_11910"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEV2129312.1"
                     /translation="MLGFPNDSVTATWPERRPVPTYGQRGMIAAAHPLIVASGAEIQR
                     QSGNAVDVAVACGLAAAVVMPEMCGLGGDLFAVIHDPRTGKTEAVMGSGISPRAATDE
                     IMLRHGDTEHRLMPYQGSMSIAVPGMVDAYFRLLERWGTMSFAQVAAPSIDLARNGFA
                     ILPKGAEDLQENADLLAQDPAAAAVFLENGRPYGAGDRLVQGDLADTLETIAADGADT
                     FYRGAIAKRMLAYLNDRGNLLSAKDFAEHTTEISSPLSTTYQGYTVHQTCLPTQGLIL
                     LEALNIVEKSEITDPLDPATIHTLVEAKKLAYADRLGYAADPRFRDVPLDRLLSKEWA
                     QERFDAIDPEQAATEARHGEMHGGDTTYFATADGDGMMVSLIQSVSSAFGSGVVAGDT
                     GVVMNNRVGRGFSLIPGHPNYFEPGKKTMHTLNCYLIDDPQGRPVLVGGTPGGDGQPQ
                     WNLQMITALVDAGMDVQQAVNMPRWTSWPGTDPITIANQFELRMEFRFDEATREEMKQ
                     RGHEVKVLGAWNGGGAAQMIARNPETGVMVGGSDSRVEGFALGR"
     gene            9346..10260
                     /locus_tag="VGR22_11915"
     CDS             9346..10260
                     /locus_tag="VGR22_11915"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2129313.1"
                     /translation="MTKPATTVLEVDHLTKRYGQILALDDVTMSVPVGSIFGFLGPNG
                     AGKTTLIRILMGFMKPTSGSARVLGHDAWTGGVQARERVGYLVTADGLYPDLSGYDQL
                     DYAATISGRSPEWRQRILDALELDAAALRRRLNTYSKGMRQKLALTVAAQHRPDLLIL
                     DEPTDGLDPLIQRNFEELLRHFRDEGTTVFMSSHDLGEVERTCDQVAVVRAGKLISSG
                     TMESLKRRYSQRVEVMFEGTVPDGLCEVAGVEMVHRDGQLAVMDLDHDINPLLAFLAN
                     HRVSRMEVRPPELQEVFMSFYEREVPAG"
     gene            10257..11072
                     /locus_tag="VGR22_11920"
     CDS             10257..11072
                     /locus_tag="VGR22_11920"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV2129314.1"
                     /translation="MIAPLTQRLLRRGLLGTVMLMLGMMLFQFVNPFIADSLGGPEGL
                     ASLVEQLPPAMQVLAQMSPDFVALTGLAGYLSRGFDHPVFLILLLSAVIGFTARAIAG
                     EVDSGVLALALSRPISRLQLYMARVLSLVLIAIVFGLAGPIASRAGLLVADPTGSVRN
                     GQLLALAALIMLFAWSVGGLALMVSAMSSSTGRVVSWATGYLVIAYFVDVFAELWSIL
                     EPLRPLSLFAWLDTSGAMVDASVPVDSGLVLGIVGLVGVVAGWLVFRQRDFMV"
     gene            complement(11171..11599)
                     /locus_tag="VGR22_11925"
     CDS             complement(11171..11599)
                     /locus_tag="VGR22_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEV2129315.1"
                     /translation="MDTDSVDATPKTCPVCGHENVATARFCVKCGHAFADTTASEEPD
                     IDATQTTSIYVPIGTSAPEPPWSEPEAERTTALPVRDAIWSPSPPAASAHVPQPRKES
                     LRGLVLGIIATMIIVAVLAYYVYAAWLSESLQNNIPDWLP"
     gene            complement(11619..12029)
                     /locus_tag="VGR22_11930"
     CDS             complement(11619..12029)
                     /locus_tag="VGR22_11930"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEV2129316.1"
                     /translation="MTDAANNQPPVAFVDSSAIIALVNRGDPTHDQAVEAYHELVEGG
                     YHLFTTDYVIAETLQLLDLAFGADLARQWLRDQRLPVYHADEQDVRRAIATIVSAHAG
                     TRLSLTDAVSFVVMERLGVSDAFAVDPEFLVDSA"
     gene            complement(12052..12369)
                     /locus_tag="VGR22_11935"
     CDS             complement(12052..12369)
                     /locus_tag="VGR22_11935"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2129317.1"
                     /translation="MEDDRNLLTVAQAARILERSTEQVRRYLREERLPGQRIGGQWFI
                     DRSALREFADNARARQTFLDRVGAARTLRPLDAVIGIANGKGSDVSKGKRTFLRNAAS
                     RAR"
     gene            12518..13489
                     /locus_tag="VGR22_11940"
     CDS             12518..13489
                     /locus_tag="VGR22_11940"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2129318.1"
                     /translation="MGERTMATEETTRGPGTAMKIVEGVYGIDTGFQDRTGAISAWLL
                     VGSEGIGLIETGPSTVLDHLAAGVADAGHDIADIREVAVTHIHLDHSGGLGELMRDHE
                     GMQAWVHPVGLPHLVDPGRLVKSASRIYGDRMEELWGTITGVPEDCVTATEDRETVSL
                     GGRRVTVFDTPGHASHHIALLDETTGTLFTGDVAGVRMQGTVFPVPPLPPPDIDIDAW
                     KRSIALMREIAPARLVLTHFGPYEDVDKHLQQLEEALDEAMRIGREVLLDGGSDRMLT
                     EQLGVWVRDRIGKDADRVWASLDAANPLFMSAMGIHRVLRKNGELEE"
     gene            complement(13672..14172)
                     /locus_tag="VGR22_11945"
     CDS             complement(13672..14172)
                     /locus_tag="VGR22_11945"
                     /inference="COORDINATES: protein motif:HMM:NF024061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HEV2129319.1"
                     /translation="MASHRILDDFSEDLTADDLAIDDRNYRRSRRSSPSRASAPKRPN
                     RRPPRTEADQAFRCRKCRQFIGAPLTGGRHRNHCPNCLHSLHLDLKRPGDRRSDCESL
                     MRPRGLIERRNGEQMILHECLGCGKTQPTRVAADDNPILLMRLRPIGLPEHIDTVEFS
                     VIEETA"
     gene            14678..15640
                     /locus_tag="VGR22_11950"
     CDS             14678..15640
                     /locus_tag="VGR22_11950"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEV2129320.1"
                     /translation="MIDVMLLGIGSMVPLPGRWLSSALLRIGQSLVLLDCGEGTQVAM
                     RERHWGFRKLDAICLSHLHADHCAGLPGLFHTIGNAGKRTPLTIYGPEGTIDVIRGLR
                     AIAPHLPYDMTVTELADGAETEGPEGLRVAVAEGEHRVPVLAYRLERPRATRFQPDRA
                     RTLGVPIDQWSVLQHGEAVVVDGRTVEPHEVLGEAREGVALGFATDTRPTDAVRDVVR
                     GVDLLISEATFGDDGDLDRAIRRGHMTFREAAALARDAEAGHLWLTHFSAAIMDPDIW
                     RDNAAEVFPAVTVGHAGLSATLAFDGGYQPNTVSEGISDDPAVD"
     gene            15737..16987
                     /locus_tag="VGR22_11955"
     CDS             15737..16987
                     /locus_tag="VGR22_11955"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEV2129321.1"
                     /translation="MTSRTDWFVHDRFGLFIHFGIYSAGARHEWLKKREAMTTETYQK
                     YFDHFDPDRYDPQRWARDAKAAGMRYSVITTKHHDGFCLFDSALTDYTATNTPFGRDI
                     IAEFVEAFRAEGLRVGFCYSVIDWHHEDYTRDQVHPQSDDDAFWAGQNRDIARYRAYL
                     HGQVRELLTNYGTIDVMWFDFTPPTKSREDWQSAELAAIVRELQPDIILNDRIDLPES
                     ADFVTPEQYQPREWLTRDGERVTWEACQTLNGSWGYDRDNLDWKSPQMLVRMLVDTVA
                     KGGNLLLNVGPTGRGEFEPRARETLAAIGEWTRLHGHAIYGATAEEAYEASVDCRYTR
                     RGDRLYLHVFTWPMRHIHLEGMAGKIEYAQLLHDASEVKVTDPDPEAQVQNITPPGFE
                     GVATLELPIQRPDVLVPVIELFLK"
BASE COUNT         2948 a         5431 c         6026 g         3084 t
ORIGIN      
        1 tcgcgtgcct gttcgagcgt gacacccttg tgggcgcgga gctgagcata cgactgggcg
       61 aagcgctcgc gcagctccgg atcgaacggg ctcaggatgc acgcgccggt gaggtcgcgc
      121 ccaagccggg acgctcgtcc gcgaatgtcg ttttcgtcgc cgaggatcgt cagcgcggcg
      181 gcctgccgct ccagcagtgt gccggcagcc cgcaggatgc ggtcgtcgtc accctccggc
      241 agcacgatgt gcttgcggtt gctgcgggcg cgctccagca gctggtattc gaacatcagc
      301 ggcgtgacga tgtcttcccg cacgacgtcc agcaccgcca ggatggcctt gccgtcgaca
      361 tgtgcatcga aaatcgagag tgcgcggtcg tacttgcgct gggagtcagc cccgaagcgg
      421 ccgcgtgtgc gggagacgcg gtatgcggtc tcaaaggtgt tcaggttcgt tctcaggatc
      481 ggaaggtcga cgccgatgcc ttcgatgagc cgctcaatcg tcggggagac atcgaacccg
      541 ccgttgagga tgatgcccga gagggacggg aatgttccgg acgtgtgtgc catcagcagg
      601 gacagcagca cctcgctgcg gtcccccggc gccaccacag cagaatccgg cgtgaggcga
      661 acgagcacgt tctccatcga catcgcggcg accaccacgc tcattgcctc gcggtccagc
      721 aggccggcat cgccgcggta gaggctggca tcgagcgtct ccatcagggc gcgaagtgtc
      781 ggggcgatga gcaccgcgtc ctccggcagc gaccaaaccg gaatctgacc ggagggggag
      841 actgtggcga agatcgcccc ggtggtggcc gcgagctgtt cgggatcgac gcggttggca
      901 atcaccgcga ttagctgggc atgctcgtcg tgcagctcgg cgagcgcgat ctccgccgct
      961 tgggcgatct cctcgggcga ccgcgccgcg ccggtgagcg cctgtgcatt gctcgggatc
     1021 agctcgttct ggcttcgccc gccgagtacg agcagcactg gcgcaccgag attggcggcg
     1081 atgcgtgcgt tgtagctcag ctctgtcggc gtggagacat cggtgtaatc ggagccgagg
     1141 atgagcacaa cgtcgaattc gggctcgatt gccttgtagc gagccacgat ccgggcgagc
     1201 gcggcatcgg ggtcggcgtg gacgtcgtcg tagctcacgc cgatgagatc cggcgcgcgg
     1261 tccggcatcc agaggtgcgc gagcagcagg tcgaggatgt aatcctcgcc ggtggtgctg
     1321 cgcgagatcg ggcggaagac gccgatggtc ttcccggatc gcttgagggt ttcgagcatg
     1381 ccgagggcaa tggtcgactt tccggtgttg ccctctgcgg acgtgatgta gatattctgc
     1441 gccataccca atgttcctgt tgagcgaggc gaaccgcgac aagccgcagg ccatcgcacc
     1501 gtgctctctc aagtctagcg ggtgccagcg acgcgacccg gcgttcggtg cgtatcgtgg
     1561 cagggtagaa gagagaaccg catgtcggca ggtaacatgc agggcagtgc ggttgccggg
     1621 ccggcactcg gtacaatgct ctacaggatt cgcggaggag tggaaggggc agtgaccgat
     1681 gacggacgtg atggccgagg accagcttgt cgggggaagt ggcgagcgct cgaaggcgga
     1741 gcacctctcc gacttccagc gggagatggc ggagcagcac caggcggcgc gcgagaagca
     1801 ggaggcgcgc ggcaagcgga gtgccctcga ccgcgtacgg cagttgctcg atgacggctc
     1861 gttcaccgag ctggatgcct tcgtgcgaca tcgagcgacc cagttcggca tggctgagaa
     1921 gcgtcccttc ggggacggcg tggttaccgg cttcggcacc attgacgggc ggcaggtggc
     1981 ggtcttctcg caggatttca ccgtcttcgg cggctctctg ggtgaggcat tcgccgagaa
     2041 gttggtcaag ctcatggagc tcgccgagcg ctgtggctgc cccatcatcg gcatcaacga
     2101 ttccgccggc gcgcgcattc aggagggtgt cgaggggctc gccggctatg gcgacgtgtt
     2161 cttccgcaat gtccgcctct ccggtgtggt gccgcagatc tcgatcattg ccggtccctg
     2221 cgccggaggc gcggtctact cgccggtgat gactgacttc acctacatgg tcgagggcgt
     2281 gggccggatg ttcatcacgg ggccggacgt catcaagacc gtgaccggtg agagggtcga
     2341 ccctgaagag ctcggtggcg cggcgaccca cacgggcacc agcggcgttg cgcacttcac
     2401 tgccacggac gaggacgatc tcttcgacca ggtgcgctac ctgctctcat tcctgccgtc
     2461 caacaatctc gacgacccgc cgtggttcgc ccccgaggac gaccccgatc gggagtcgga
     2521 ggcgctggac agcctcgttc cggaggccag caacaagccc tatgacatgc accgagtgct
     2581 cggcgaggtg ctggacgacg gcgagttcta cgaggtgcag cagaatcacg cgcagaacct
     2641 gatcgtcggc ttcgggcggt tggacggcca cccggtcagt gtggtcgcca accagccgag
     2701 cgtgctcgct gggacgctcg acatcgacgc cagcgtcaag ggcgctcgct tcgtgcgctt
     2761 ctgcgatgcc ttcaacctgc cgatcatcac cttcgtcgac gtgccgggct tcctgccagg
     2821 gaccgatcag gagtatcgtg gcatcattcg gcatggctcc aagttgctct acgcctactg
     2881 tgaggcgacc gtgccgaagc tgacggtgat catccgtaag gcctacggtg gcgcgtatgt
     2941 cgtgatggcg agcaaacaca ccggggcgga cttcaacgtc gcttggccga caggcgaggt
     3001 cgcagtgatg ggtccagagg cggcggtcgg gctggtcttc cgccgggagc tcgccggtct
     3061 ggaagatccg gatgcccgca agaaggagct ggtgcaacag tacgaagacc tctttgccag
     3121 cccgtaccag gcggcgtccc gtggatacat cgacgacgtg attgagccac acaccacccg
     3181 accatggctg atccggtcac tggcgctggc gcggagcaag cgggtggcga cgccgcgccg
     3241 caagcatggg aacatccccc tgtgagtggt aacgcgcacc gtgatacagg catcctaatt
     3301 cgcgtgatgc cggagccgac gcctgaggag ctggcagcaa tcgtcgcggt cctgcgcgcc
     3361 cgtcgtcgcc gacagcagac gatggcaaca accgaggagc tgtcgcattc ggtcagccgg
     3421 tgggcccgtg ccggtcgtga ggaagcgatg gccgcgtggg atttcggcga tggaaagggc
     3481 accgggagtt ggtgacagta gtcgatcgcc ggaggcaacg gcgccggcaa acccgcaccc
     3541 cggcgcgaca gtgggcgggg ttcacacgtg accggcggtt cccgatacgt gaaatcgcat
     3601 gatggacccc gggcattccg ggttggggac tccccgaatc tgcgctgtcc ctgcagccac
     3661 acatcggcac catcagcgtt ctcccaccca cagaggagcg ggcctacctg tagtctgcca
     3721 cgtcgatctg gttcgggctc gagaaaggcg ttcggcgggc ctggcaccgc ctgaggagtt
     3781 cgccgcgggc gacgaggatg cgcgcgtgca gctcgggatc gttcgtcagc agctcggcct
     3841 cctcgagctc ctcctcgtcg agcacggcga cctcgccgtc gggcgtcaca acgatgtcga
     3901 tgaagaggtc gttccagacg acttccagct gttcacctga tgccgtcaca acagtcggat
     3961 agacgacgtt cgcgtaccac cccttgagct cgcctgctgg tgagtagact gcgaaggcat
     4021 tgtacgggtg gatgggggag aagatttccc gcaggacgtc gtcaatctcg aacgtgaggt
     4081 gggcctgctg gacggtccct gcaacccaga acgcctggat ctccacccat ggcgccggca
     4141 cggcggtggt gacgacggtg ccggggtatg caacacggaa gtcagggccg tgcatgactg
     4201 agtgtttgac gacggttacc tcccggccag gaaccagcgt gggccagcgg tcaagcgtcg
     4261 ggccatcgat gccccagagc ttggcctcgg ggcggtcctc gatcgtgatc cggtcagtcc
     4321 gcatcgtcgg tcagcagctt ttcgacgatg acgacgtccc gccactcgcc atccagcttc
     4381 gcgtgcctgc ggtaggtgcc cacctcacga aaaccgagcg agcgcatcag gcgcaggctc
     4441 gacgcgttct ctgggaagat gcgggaaatc agtttccaga accctgtttc gctggcggca
     4501 tcgaagaggg cttccatcgc gatccggccg acgccgcggc cgcgcgcgct ccgtgcgaca
     4561 tagacggagt actcggcgat gccgtcgtag cactggcgcg gccggtaggt ggaggtcgac
     4621 gcgaacccga cgacctctcc gccgatctcg accaccacca ccgggtggcg accatcgaac
     4681 cacgcctcga tgtcggctgc ggtgcggtgg agtgtctcga aggtcgcgac tcgatcgtcg
     4741 atgccttcgt tgtagatacg cgcgatcgcc tccgcatcgg tcggattcgc cgcgcgggcg
     4801 gttggtgcga ccgtcgacac gctcattggc ggatcagctc cagaacctcg ctggtcttct
     4861 cccggaggag ttcctccgtc tccgcctcga cgttcaggcg caatagcggc tgcgtattgg
     4921 aggcgcgaag gttgaaccac cagtcgtcga atccaaccgt gagaccatcc aggtggtcga
     4981 tctcggcgcc cttgtcgctg taaaaggcct cgacccgcgc gacggtgccc tcgatgtcct
     5041 ccacctcgga attgatctcg ccggagcgaa cgcgggtatc aatggggtcg aggatctccg
     5101 agagcggctg gtcggcgtcg gagatcagct ggagcacggt cagcgcggcg atcacgccgg
     5161 aatcggcgta ccagttatcg cggaagtaga agtggccgga gtgctcgcca ccgaagatcg
     5221 cgtcttcctc gcgcatcacc gccttgatgt agctgtgacc aacgcgcgag cgaatggcat
     5281 ggccgccgtt ctcctcgatc agctccggta cgctgcgcga gcagatcagg ttgtaaacga
     5341 tggtcgagcc cggattgtgc ttgagcagct cgagcgacac catcgcggtg gtcatgtcac
     5401 caccgataaa gttgcccttc tcgtcgatca ggaacatgcg gtcggcatca ccatcgaagg
     5461 cgatgccgag gtcgcagccc tcgttgacca ccgtgcgctc gaggtcgcga acgttctccg
     5521 gctcgatcgg gttggcctca tggttgggga aggtgccgtc cagctcgaag taaaggggca
     5581 cgatctcgca tggcagtgtg tcgaagactg ccggcacgag cttgccgccc atgccgttgc
     5641 cggcgtcgac ggcgaccttg agcggcttga tacggctcgg atcaatcatc gagagcacat
     5701 gctcggcata ggcctccagc acgttcttct cggtcaggtc accacgcttg ccgcgcggtt
     5761 tggggaagtt gcccgagacc accatgtcgc gaatctcgcc gatgccggta tccagcgaga
     5821 gggcacgggc atcttcccgg cagaccttga agccgttgta gttggcgggg ttgtgcgagg
     5881 cggtgatcat gaccccggcc gggaagccgt acttgccgac cgcgaagtag agggtgtcgg
     5941 tggagacgag cccgatgtcg acgacatttg cgccctgatc gcgaatgcca tccatcaggg
     6001 ctgcctgcat ggccggaccg gagacgcgca tgtcacgccc gaccaccacc tcgtcggcat
     6061 cgaggaaggc gaccacgccg cggccgatgc ggtaggcgat ctcgggcgac agttcgtcgg
     6121 gcacgattcc tcgtacgtca tatgccttga acagcttgcc gaccgagttg tccaatggct
     6181 cgagggatgc ctgtgtcgtg tcagtcatga gcaggactac ctccggtgat actgacgtta
     6241 gggaacggag ttgttccccg gatgtcgtgt acgatcccta tcatacgtgt ttcgccgttt
     6301 ccggccattt gccaggccgc aagtcgttca ggaacaccac cgcatgttgg atgcaaccga
     6361 tattcgtctc cgcgccgacg acccgatcga tctgcacctg cataccttcc aaagcgacgg
     6421 cgtctggacg ccgatgcagt tgatcgacca tctcgccgag gccgggatca ccgtggcggc
     6481 cgtctgcgac cacgagaccc agcgcagcgt ggtcgaggct atcgagtacg gcgagcggcg
     6541 tggaatctat gtgatccccg gcgtggaagt caccgtccga cacgatgacc ggcagtggca
     6601 catgctgatc tacggcatcc gcccggatga tgatcgtgcc gaggcgcggc cattcctgga
     6661 gatgatgcga gagcatgagg cgagcctgta cagtctcgcc cgggcgaact gggcacgggt
     6721 cgagcggacc gggcgcgtat tgccgtccct tgagacgatc gtcggcgacg gcaagatgtg
     6781 gacctatccc gaggtgctcg tcgcgatgat ccgggacggt cacgcgccca atctcaccat
     6841 cgcggcgaac atgatcaccg agctcggcgg gcgattcaac gcggacattc cgatcgagga
     6901 ggtggtcagc aaggcccacg aggccggggg cgtctgcgtg atggcgcacc ccgggcgccc
     6961 ggacctcggc ccggcgatga ccgccgagac gctggcgaag ctgctcgccg acgcgccagt
     7021 cgatggactg gagtgccact accgcaccta caccgatcgt gacacggcct tcttccggag
     7081 cctcgctggt cagcatgggc tggtgacctc ggcggggtcg gattcgcacg ggctgaatac
     7141 gccggtcaac ccgcgtccct tccgagcagt gtgggcaaag tcgctgctgg aacggttggg
     7201 ggttgtggtg gatgacccag tggacgagcc ggtgtgggcg cccgggatgg acccgctcgc
     7261 cgtcaagccc cagcggaaga agcggggcaa gtggaagagg cgccagaggc tgggccggtt
     7321 ctggcgccgc aaacgagcgt aggggcgctg gaggcggagt cgcacctcgc tggtagcgat
     7381 cgtgtggtgg acatgtcatc tcgagcgaag ggcgagtgca acgagcccgg agtcgagaag
     7441 tcgtgtggtt ggcgaacgac ccaccgtcgc cagccacggg attgctctac gcggcgggct
     7501 gatgcacgcc ttggtcgaaa tgatcatggc tttccgaggg tggccgctcc ttcgcgttgg
     7561 gacgcccgtt ctatcggccc agcgcgaagc cctcgacgcg ggagtcggag cctccaacca
     7621 tgacgcccgt ctccgggttg cgcgcaatca tctgcgctgc tccgccgccg ttccacgccc
     7681 ctagcacctt cacctcgtgc ccgcgctgct tcatctcctc gcgcgtcgcc tcgtcgaaac
     7741 ggaattccat gcgcagctca aattggttgg cgatggtgat cgggtcggtg cccggccagc
     7801 tcgtccagcg cggcatgttg accgcctgct ggacatccat gccagcgtcg accagcgccg
     7861 tgatcatctg caggttccac tgtggctgtc catctccacc cggcgtgccg cccacgagca
     7921 ccggacggcc ctgcgggtcg tcgatcaagt agcaattgag cgtgtgcatc gtcttcttgc
     7981 cgggctcgaa gtagttcgga tggccgggga tgagcgagaa gccgcgcccc acgcggttgt
     8041 tcatcaccac gccggtgtca ccggcgacca caccgctgcc gaaggcgctg ctgaccgact
     8101 ggatgagcga gaccatcatg ccgtcaccgt cagcagtggc gaagtaggtg gtgtcaccgc
     8161 cgtgcatctc gccgtgccgt gcctcggtgg ctgcctgctc cgggtcgatt gcgtcgaacc
     8221 gctcctgcgc ccattccttc gagagcagcc gatccagggg gacatcgcgg aacctgggat
     8281 cggcggcgta gccgagacgg tcggcgtagg cgagcttctt tgcctccacg agcgtgtgga
     8341 tggtggccgg atcgagcggg tcggtgatct ccgatttctc gacgatgttg agcgcctcca
     8401 gcaggatgag tccctgcgtc ggcaggcagg tctggtggac cgtgtagccc tggtaggtag
     8461 tcgagagcgg tgaggaaatc tccgtcgtgt gctcggcaaa gtccttggca ctcagcaggt
     8521 tgccgcggtc gttgaggtag gcgagcatcc gcttcgcgat cgcgccgcgg tagaaggtgt
     8581 cagcgccgtc cgcggcaatg gtctccagcg tgtcggcgag atcgccctgc acgagcctgt
     8641 cgccggctcc gtacggcctg ccgttttcga ggaagacggc cgccgcagca gggtcctgcg
     8701 ccagcaggtc agcgttttcc tgcagatctt ccgcgccctt cggtaggatc gcgaagccat
     8761 tgcgagcgag gtcgatcgac ggggcggcaa cctgcgcaaa ggacatggtc ccccatcgct
     8821 ccagcagccg gaaataggca tcgaccatac cggggacagc gatcgacatc gatccctgat
     8881 aaggcatcag gcggtgctcg gtgtcgccgt gccgcagcat gatctcgtca gtggccgcac
     8941 gtggcgagat gccgctgccc atcacggcct cggtcttgcc ggttcgcggg tcatggatca
     9001 cggcgaagag gtcgccgccg aggccacaca tctccggcat caccaccgcg gcggcaaggc
     9061 cgcatgccac cgctacgtcg acggcgttgc cactctgccg ctggatctcg gcaccagatg
     9121 ccacgatgag cggatgagcg gcggcgatca tgccgcgctg gccgtaggtc ggtaccgggc
     9181 ggcgctcggg ccaggtcgcg gtaacggaat cgttcgggaa gccgagcatg gtgtctcctc
     9241 tgcaacaggg atgcggacga acatcgtgcg atcataatgc caacgatgca tgatgctggc
     9301 gaccccaccg gagccagcga cgattgctgg gagtagagag ccacaatgac caaacccgcc
     9361 acgaccgtgc tcgaggtgga tcacctgacc aagcgctacg gccagatcct cgccctcgac
     9421 gacgtcacga tgtccgtgcc agtgggctcg atcttcggat tcctcggccc gaacggggcc
     9481 ggcaagacta ctctgatccg catcctgatg ggcttcatga agccgacctc cggtagcgcg
     9541 cgggtgctcg gtcacgacgc gtggactggc ggcgtccagg cccgggagcg ggtcggatat
     9601 ctggtcactg ccgatggcct ctaccctgat ctcagcgggt acgaccagct cgactacgcc
     9661 gccaccatca gtgggcgatc gccggagtgg cgccagcgga ttctcgatgc gctcgagctg
     9721 gacgccgccg cgcttcgtcg tcggctcaat acctactcga aggggatgcg gcaaaaactt
     9781 gcgctcaccg tcgctgcgca gcaccggccc gacctcctga ttctcgacga gccgaccgac
     9841 ggactcgatc cgctcatcca gcgcaacttc gaagagctgc tgcgccactt ccgcgacgag
     9901 ggcaccaccg tgttcatgtc ctcacacgat ctcggcgagg tcgagcggac gtgtgaccag
     9961 gtcgccgtgg tgcgagcggg caagctgatc tcgtccggga caatggagtc gctcaagcgg
    10021 cgctactccc agcgcgtgga agtgatgttc gaggggacgg tgccggatgg tctgtgtgag
    10081 gtcgccggcg tggagatggt gcaccgggat ggacagctgg cggtgatgga cctcgatcat
    10141 gacatcaacc cgctgttggc gtttcttgcc aaccaccgcg tgtcacgcat ggaggttcgt
    10201 cctcccgagc tgcaggaggt cttcatgagc ttctacgagc gcgaggtgcc ggccggatga
    10261 tcgcgccgct gacacagcgt ctgttgcggc gcggcctcct cggcacggtg atgctcatgc
    10321 tcgggatgat gctcttccag ttcgtcaatc cgttcatcgc cgactcgctc ggtggtccgg
    10381 aggggttggc gagtctggtc gagcaactgc ctccggcaat gcaggtgctg gcgcagatga
    10441 gcccggattt cgtcgccctg accggactgg ccggctatct ctcccgcggg ttcgaccacc
    10501 cggtcttcct gatcctcctg ctttcggcag tgatcggttt caccgcgcgc gcgatcgctg
    10561 gcgaggtcga cagcggcgtt ctcgcgcttg cgctctcgcg gcccatctcg cgcttgcagc
    10621 tctacatggc gcgagtgctc tcgctggtgc tgatcgccat cgtcttcggg ctcgctggtc
    10681 caatagcgtc gcgagcgggg ctgctggtcg ccgacccgac cgggtccgtc aggaacgggc
    10741 agttgctcgc gctggcggcg ctgatcatgc tctttgcatg gtcggttgga gggctggcgc
    10801 tgatggtttc ggcgatgtcg agcagcaccg ggcgggtggt gagctgggcg actggctacc
    10861 tggtgattgc ctacttcgtc gacgtcttcg ccgagctgtg gtcaattctg gagccgctgc
    10921 gcccactgag cctctttgcg tggctcgaca cgtcgggggc gatggtggac gcttccgtgc
    10981 cggtggactc ggggctggtg ctcggcatcg tcggcctggt cggggtcgtg gcggggtggc
    11041 tggtctttcg ccagcgagat ttcatggtct gacgtcgggg acggtgagga catcccgaaa
    11101 agcgggggga gagccgtcat tcttagcgaa acggggccgc tcctgtgaca tggcgaaccg
    11161 cccgtgcctg tcagggcaac cagtcaggga tgttgttctg gagggattcg ctgagccagg
    11221 cggcatagac gtagtacgcc agcactgcaa cgatgatcat cgtggcgatg ataccgagca
    11281 ccagaccgcg cagcgattcc ttccgcggct gcggcacgtg agcggaggct gccggtggcg
    11341 acggcgacca gatagcgtcg cgcacgggca gggcggtggt ccgctccgct tccggctcgc
    11401 tccatggtgg ctcgggcgcg gatgtgccga ttggcacgta gatgcttgtg gtctgggtcg
    11461 cgtcgatgtc cggttcctcc gacgccgtgg tgtccgcgaa tgcatggccg catttcacac
    11521 agaatctggc ggtggcgacg ttttcgtgcc cgcagacagg acaggtcttc ggggtggcgt
    11581 cgaccgagtc ggtgtccatg gcagatgctc tttccggatc aggcgctgtc gacgaggaac
    11641 tcgggatcga cggcgaacgc atcggagaca cccagccgct ccatcacgac gaagctgacc
    11701 gcgtcagtga gtgagagacg ggttcccgcg tgcgcgctga cgatcgtcgc tattgcgcgg
    11761 cgaacgtcct gctcatctgc atggtagacc ggcaggcgtt ggtcccggag ccactggcgg
    11821 gcgagatcgg cgccgaatgc caggtccaag agctgcaggg tctcggcgat gacgtagtcg
    11881 gtcgtgaaga ggtggtaccc accttcgacc agttcgtggt aggcctccac ggcctgatca
    11941 tgtgtcgggt caccgcgatt gaccagcgcg atgatcgctg acgaatcgac gaacgccact
    12001 ggcggttggt tgtttgcggc atcggtcatg gtaggctcct gacggcgcgg gctagcgcgc
    12061 gcggctggcg gcgttgcgga gaaaggtgcg cttgcccttc gagacatcgg aacccttgcc
    12121 attggcgatg ccgatcacgg catccagcgg gcgcagtgtg cgggcggctc cgacgcggtc
    12181 aagaaaggtc tgccgggcgc gggcgttgtc cgcgaattcg cgcagcgctg atcggtcgat
    12241 gaaccattgc ccgccgatcc gctggccggg gagccgctcc tcgcgcaggt agcggcgcac
    12301 ctgttcggtc gaccgctcca gaatgcgcgc cgcttgggcc acggtcagca ggttccggtc
    12361 gtcttccacg atctgtctcc ctgggtctct cgcgcatacc agcggagtcg cgcgcgtcgc
    12421 ggaagctagg catgcctggc cgaaggtggt aggtgttgtt gtcgacgaca tggtagtgac
    12481 aacctgcagg tcgggcaagg gggtgtccga tccgcccatg ggagagcgaa cgatggcgac
    12541 ggaagagacg acgcgcggcc ccggcaccgc aatgaagatc gtggagggtg tctacggtat
    12601 cgataccggc ttccaggacc gaaccggtgc gatcagcgct tggctgcttg tcggttcgga
    12661 ggggatcggg ctgatcgaaa ccggaccgag tacggtgctg gatcacctcg ccgccggcgt
    12721 cgccgacgca gggcacgaca tcgcagacat cagggaggtc gcggtgacgc acatccatct
    12781 cgatcactcc ggtgggcttg gcgagctgat gcgcgaccat gaagggatgc aggcgtgggt
    12841 tcatcccgtc gggttgccac atctggtcga tcccgggcgg ttggtgaaga gcgcgagtcg
    12901 catctacggt gatcgcatgg aggagctttg ggggacgatc accggggtgc cggaggactg
    12961 tgtgaccgcc accgaggacc gagagacggt ctcgctcggc ggtcgacggg tgacggtctt
    13021 cgacactccg ggccacgcct cgcaccacat cgcgctgctg gacgagacga ccggtacact
    13081 cttcaccggg gatgtcgccg gcgtgcggat gcaggggacc gtgttcccgg tgccaccact
    13141 gccaccaccc gacatcgata tcgatgcctg gaagcggagc atcgcgctga tgcgggagat
    13201 cgcgccggct cggctggtgc tgacccactt cgggccgtac gaagacgtgg acaagcatct
    13261 gcagcagctc gaagaggcac tcgacgaggc gatgcggatt ggccgagagg tgctgctcga
    13321 tggcggcagc gaccgcatgc tgaccgagca gctcggcgtc tgggtgcggg accggatcgg
    13381 gaaggacgcc gatcgggtct gggcatcgct ggatgcagcc aacccgctct tcatgagcgc
    13441 gatgggcatc caccgggtcc tccgcaagaa cggcgagctg gaggagtaga cggcaccgtt
    13501 gctcggcgtg ccatcgcagg agaaaaccgt agggggcgac cctgtgtcgt cccggtcacg
    13561 cggcgagcat ccaatccgtg ggcgagaaac ccatccacgt atcgtcgtcc tgtggcgggc
    13621 cgggctggcg cagcggccag cccctacggt tgcatcgaag cgaaacccgt gtcaggcggt
    13681 ctcctcaatg acactgaatt cgaccgtgtc gatgtgttcc ggcaggccga tcggccgcag
    13741 ccgcatcagc agtattgggt tgtcatcggc ggcgacgcgc gtcggctggg tttttccgca
    13801 gccgaggcac tcatgcagga tcatctgctc accatttcgg cgctcgatca gtccacgtgg
    13861 ccgcatcagc gattcgcagt cgctgcgccg gtcaccgggc cgcttcaggt ccaggtgtag
    13921 tgagtgcaga cagttcgggc agtggttccg gtggcgaccg ccggtgagcg gcgcgccgat
    13981 gaactgtctg cacttccggc agcggaacgc ttggtccgcc tcagtgcgcg gcgggcggcg
    14041 gttcggccgc ttcggcgctg atgcacggct cgggcttgat cgccgcgagc ggcggtaatt
    14101 gcggtcgtcg atcgcaagat cgtcagcggt caggtcctcg gagaaatcgt cgagaattcg
    14161 gtgcgaagcc aacggcgcat cctccgcacc caccgtggtg ggcgcgcatg aagggaagat
    14221 ggacaaggtg ggatgcccgc gacgcagtgg cagcggtcgc ggtggctcgc acaagagcgt
    14281 tcatggatgg catggtgcac ctcgggtagc gttcccacag ccgacgggca aggggagacg
    14341 ccggtaaaac gggacggaac gacagaggga cgcgatcggt cctcgccgga acggcccgtg
    14401 ccggttcagg tacctgagtt ggtgcttcct aggtgatgga tgccgccggt cggtggccgg
    14461 ctggcggaaa ggggctggag ggaagcggtc agtgcaggtc gaacctggcg gcggccagtc
    14521 gggcacgcgc agtgatcgtg gttcggcagg ttcgctgttg cacaaagccc tccagttcgt
    14581 caggtgcggt ggcacactga atacgacggt acgcagcttg cccaaatgcc atcgtatcgg
    14641 agcgatggcc gaaaggcaag gggggcaaga ccaattcatg atcgacgtga tgcttctggg
    14701 aatcggctcc atggtgccgt tgccgggccg atggctctcc agcgcactgc tgcggatcgg
    14761 ccagagcctg gtgctgctcg attgtggcga ggggacgcag gtggccatgc gcgagcggca
    14821 ctgggggttc cgcaagctgg acgcgatctg cctcagccat ctccacgccg accactgcgc
    14881 cggcctgccc gggctgttcc acacgatcgg caacgccggc aagcgcacgc cgctgacgat
    14941 ttatggcccg gagggaacga tcgacgtcat ccgcggtctg cgcgctatcg cgccgcacct
    15001 gccgtatgac atgaccgtga cggagttggc tgatggagcg gagaccgagg gcccggaagg
    15061 gcttcgcgtc gccgtcgccg agggcgaaca ccgtgtgccg gtgctcgcgt accggctcga
    15121 gcgccctcgt gcgacacgct tccagccgga ccgcgcccgc acgctcggcg tgccgatcga
    15181 tcaatggtcg gtgctgcagc acggcgaggc ggtcgtggtc gatggacgca cggtcgagcc
    15241 acatgaggtg ctcggcgagg cgcgtgaggg cgttgcgctc gggtttgcga ccgacacccg
    15301 accaaccgac gccgtgaggg acgtggtgcg cggggtcgac ctgctgatct cggaggcgac
    15361 cttcggtgac gatggcgacc tcgacagggc gatccgacga ggccacatga cgtttcgcga
    15421 ggcggcggcc cttgcccgtg acgccgaggc cggacatctc tggctgacgc acttcagtgc
    15481 agcgatcatg gacccggaca tctggcgtga caacgccgcc gaggtcttcc cagccgtcac
    15541 ggtcggtcat gccgggctct ctgcgacgct tgccttcgat ggcggctacc agcccaacac
    15601 tgtatcggaa gggatcagcg acgatccagc agtggattga cggcaaactg gcctgcgggt
    15661 agtgggccgc taggtgtgac gtgttacggg ctgtatacgc gcaagggacg acccttcgtc
    15721 agggagcgag atcgccatga catcgcgtac ggactggttc gtccacgatc gtttcgggtt
    15781 gttcatccat ttcggcatct actcggccgg tgcccggcac gagtggctca agaagcgcga
    15841 ggcgatgacg accgagacgt accagaagta cttcgaccac ttcgatcccg atcgctatga
    15901 tccccagcgc tgggcgcgcg atgccaaggc ggccggcatg cgctactcgg tgatcaccac
    15961 caagcaccac gacggcttct gcctgttcga ctctgcgctg accgactaca cggccaccaa
    16021 cacgcccttc ggccgcgaca tcatcgccga gttcgtcgag gcgttccgcg ccgaggggct
    16081 gagggtcggg ttctgctact cggtcatcga ctggcaccac gaggactaca cccgcgacca
    16141 ggtgcatccg cagtccgacg atgacgcctt ctgggcgggg cagaaccggg acatcgcccg
    16201 ataccgtgcc tacctccatg ggcaggtgcg cgagctgctc accaactacg gcacgatcga
    16261 cgtgatgtgg ttcgacttca ccccgccgac caagtcgcgg gaggactggc agtcggcgga
    16321 gttggcggcg atcgtgcgcg agctgcaacc agacatcatc ctcaacgacc gcatcgatct
    16381 gcccgagagc gccgacttcg tcacgccgga gcagtaccag ccgagggaat ggctgacccg
    16441 cgacggggag cgggtgacct gggaggcgtg ccagacgctc aacggctcgt ggggctatga
    16501 ccgggacaat ctcgactgga agagcccaca gatgctggtc cggatgctcg tcgacacggt
    16561 ggccaagggt ggcaatctgc tgctcaacgt cggtccgacc gggcggggag agttcgagcc
    16621 gcgggcgcgg gagaccctgg cggcgatcgg cgagtggacg cgcctgcacg gccacgcgat
    16681 ctacggcgcg acggccgagg aggcgtacga ggcctcggtc gattgccgat acacgcggcg
    16741 gggggaccgg ctctacctgc acgtcttcac gtggccgatg cgacacatcc acctcgaggg
    16801 catggccggc aagatcgaat atgcacagct gctgcacgat gcctcggagg tcaaggtgac
    16861 ggatccggac cccgaggcgc aggtgcagaa catcaccccg ccggggttcg agggggtggc
    16921 gacattggag ctgccgatcc agcgcccgga cgtcctcgtc cccgtcatcg agctgttcct
    16981 gaagtagccg gcctgtcatc ccgtccagcc cggcacaccc acacctccgc ggacgggcaa
    17041 cgttgcggga accgagatcg actgccaatt gtgaacgccg gacctgctgg cctgtagact
    17101 cgagcctcgg tgcgtgggtt tcactatttg gtaacgatgt tcgattccga tggcaacacc
    17161 gagggagccg aacggtagcg ccgaccttgt gtcggccgtt cgtcacttga agtcgcgctc
    17221 ccttcgcgcg atgcagacct tcatagtcgg aaccctgaca atcggttctg agcgccacag
    17281 gcaggcggcg atgaacgaaa atgcgcgcat ctgaagcggc ggatccacgt cggatcgggt
    17341 cgagctgccc acagggccgt tgcgccctca cgcatccgtg tcccgcctca ggatccggac
    17401 gccgtcctcg ctgaagacct cggcccagcc ggggccgggg aactccgggg aggtcgcctc
    17461 ggggaatggg ccgggcgcgc aggtcggcc
//
LOCUS       DASYWL010000340         7951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_112095, whole genome shotgun sequence.
ACCESSION   DASYWL010000340 DASYWL010000000
VERSION     DASYWL010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7951
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112095"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="VGR22_11960"
     CDS             <1..852
                     /locus_tag="VGR22_11960"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEV2129322.1"
                     /translation="ALLLGASSSARQVPTDEVAPEEGPRPGAIGEPTAPVSSKHLYDT
                     ANLLNDAQEAKIEADTSRLQRFGLPVVILVQLNELSPNEASAFAAEVRRQWAVETEPG
                     ADDGLVMLVSINTVAGSISTTLSWGENALPNDGLNDALAATIDSEWLDAAIAESQLYE
                     GILFSLRRMIYHSIYAPAPPAPLTTVQRITNATVDVVGPLLGLTGIAIAVAALRFRAS
                     QRAPAPVLVWSVGAAAVLVAVAAVWSRSGWGAAASIALLGSTAVLWVARDPAAHPSRP
                     LGGSVMP"
     gene            849..2174
                     /locus_tag="VGR22_11965"
     CDS             849..2174
                     /locus_tag="VGR22_11965"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEV2129323.1"
                     /translation="MIPPTGTSTNRATSRLWLVIPALLFLLSLAMNLNDVPTTEFHPD
                     ETRWINRAHYFPDLLDPFGPTWEDQYLTRGQPPLGSYLMGLGLLLQGRDTETNGVWDF
                     AYGSEWNRLSGAMTEEADLIAARRTNAVIAAITVVVVFFVGRALTGTIAGTAGGLLLA
                     VHPLHIWIGSQALSDQLLILIIALTVLTAIHLGRGPSRGKALLLGILLGLGGAVKLSP
                     LLLSIPVALYGAALLLLARGGLVDRAKARRLAPLLLVQPIIAAITFVASYPYLWPAPI
                     DRTINLFELRTQEMEGQASAWPQVAIAHPGEGLLRVYDRLTWQSSATGNLAEDILRRF
                     GIESDVWGIDLLLAVLGIAALAWLVIRRGLTSGTALAAYVLAGQIAGIIVGMRVDFYR
                     YHLPIVLAVAILAGVAVQRVWALLVSRATHDQRSVSGSTGQPMIHTGSR"
     gene            2174..3538
                     /locus_tag="VGR22_11970"
     CDS             2174..3538
                     /locus_tag="VGR22_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129324.1"
                     /translation="MPRKVSFTPLAAILAGLAIFGAVETVQFVTADHDGWDIFAPVSV
                     TTGPLVVDPRNMLDGEDREVIARVVRETRDYGVPWSIQVIRPQGSMGNVSAQEFAEQQ
                     YAEQPVESVEDAADGLLMLVSVPEDPTETEIAFAAGPNFYPRGGLTPERLAETVGTQT
                     AQAIEEKRIGDAVIEAATWVAWMHLFRSTPEEPATHLERGLQELLQPLGAVGFAALAL
                     LVLAAAAATVVMTRLHTSTTAVPSKLDGVLAGAIARGRVDRPVLVGAVLDAIDRGVLT
                     ITPDGTVAPGPVQPATERDRLIAATLGDRPAPIRGLLHTLDRRAQLVRLMEDVLAAAG
                     AFHPRSRVLTLWLRWIAVAGLIVGVLAIVVSVLGSARYALVAALALAVISLVVAIWNE
                     RRSWATRTGKRAVAKWRDLHQDGEDRQRTMFETITGMESLAATRSGEAPVSPAAWQVL
                     PDVH"
     gene            complement(3718..5904)
                     /gene="glgP"
                     /locus_tag="VGR22_11975"
     CDS             complement(3718..5904)
                     /gene="glgP"
                     /locus_tag="VGR22_11975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEV2129325.1"
                     /translation="MVMSQPSYTVEPPERISRLNDIAYNLWWTWSNPARLLFKELHPV
                     LWDVVEHNPVLFLHRIDRHRLEAAANNPAYLARYDRVLAAFDRMQSEDVSTTWLGLNR
                     PDLVDKPVAYFSAEFGLHRALPIYSGGLGVLAGDHVKEASDMGIPLVGVSLLYRQGYL
                     RQRIDHHGWQHDVPADLNPGSEPTTQVLNDDGTPLHIEVDLHDGTAPLRLAVWQVRVG
                     RVSIYLLDSDVEGNPEWTRQLSSRLYGGDREHRLRQEIVLGIGGVRVLRALGINPVYW
                     HANEGHAALHMVERVREHVVAGDTAEVAIDKVRRSTVFTTHTPVPAGHDIFSDAMMDR
                     YFSQYWGELGVDREAFLALGRMADEPGFNLTALSMRLAAHVNGVSKKHGEVSRSMWQE
                     LWDDADSDDVPIFSITNGVHLRTWVSPIVRPTYDSYLPSTWRNQQENPLVWETIRNIP
                     DKEFWEAHQLAKQALIDEMDERARRRYAEGQFDAWQVLSSGSFQNANVLTIGFARRFA
                     TYKRATLIFQDMDRLARILTNPDNPVQLVFAGKAHPADEGGKSLIQEIYTRARDPRFE
                     GRIGFAEDYEMGLASLLVAGVDVWLNNPRYPMEASGTSGMKAAANGVLNCSILDGWWI
                     EGYEADESNGWGIPPSGLEGEAQDEAEANAIYETLERKVVPLYYQRDADGLPREWIAR
                     AKNAICTIAPQFSTRRMLIDYVTKLYGPAASGAEIDARMRGLVAAR"
     gene            6186..7595
                     /gene="mptB"
                     /locus_tag="VGR22_11980"
     CDS             6186..7595
                     /gene="mptB"
                     /locus_tag="VGR22_11980"
                     /inference="COORDINATES: protein motif:HMM:NF038066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol phosphomannose-dependent alpha 1,6
                     mannosyltransferase MptB"
                     /protein_id="HEV2129326.1"
                     /translation="MLAGALLLLVAFPFDGNQDALVDLRLMSDASEQGLLVWTVALVL
                     LTGGWVGGILAARGRALRKVRLPVTAFTVLASVAMLLTYPGTAIDVYIYAARSHLLTD
                     LGLDPSTTLPETLWDIDPYVRYASNEWANDPSPYGPLWNLIAAPATAFDDENIAVAVV
                     IFKAIMAAGVAITGWLIHDIARRVQPGLAASATIGWLWSPVVLWEGIANGHNDVLLAL
                     LLVTALWCWYRGYLGSLLPLLGAASLLKVVAMITIPAAAVAIIRRTGWNRALAAIAGR
                     AALFSLLIAWVAFAPFYDVAGVIDAIRAQTTVIVTSPALLAQAISNELGWGLDVGEDF
                     PTFGVPMIILLTLAGAIAAWLRPSQFPTIAYEQLFWFLVLATSNLRPWYTIWLVALGL
                     ALPLGMPFARAAAFAIGGLGTYAYLLWVRHWYAAEWLVEVGITVGIMLGPTLLVTLWA
                     AFSPLHRRLRQAHAVPTEA"
     gene            complement(7631..>7951)
                     /locus_tag="VGR22_11985"
     CDS             complement(7631..>7951)
                     /locus_tag="VGR22_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2129327.1"
                     /translation="QKAGTLSGGEQQMLAIGRALMARPRVLLLDEPSMGLAPILVEQI
                     FRIVQDINAQGTTVLLVEQNALMSLAIAHRGYVMQTGEIVLSDDAHVLMRDEGVRKAY
                     LGEE"
BASE COUNT         1322 a         2696 c         2492 g         1441 t
ORIGIN      
        1 gccctgttgc tcggcgcctc aagcagcgcc cgacaggtgc caacagacga ggtggctccc
       61 gaggagggac cgcggcctgg tgccattgga gaaccaacag cgccggtcag ctcgaagcac
      121 ctctacgaca ccgccaatct gctgaacgac gcccaggaag cgaagatcga ggcagacacg
      181 tcacgcctcc agcgtttcgg cctgccggtg gtcatcctcg tgcagctcaa cgagctctcg
      241 ccaaatgaag cctcggcctt cgcggccgag gtgcgccgtc agtgggcagt ggagacagag
      301 ccaggcgcgg acgacggcct ggtgatgctc gtctcgataa ataccgttgc gggctctatc
      361 tcaacaaccc tctcgtgggg tgagaacgcc ctaccgaacg acggcctgaa cgacgcgctc
      421 gcggcgacga tcgactcaga gtggttggac gccgcaatcg ccgagagtca actgtacgaa
      481 ggtattctct tcagcctccg ccgcatgatc taccactcga tctacgcgcc cgcgccaccg
      541 gcgccgctca ccacggtgca acgtatcacg aacgcgacgg tcgacgtcgt cggaccgttg
      601 ctcgggctga cggggattgc gatagcggtt gcagctctcc gctttcgagc ctcgcagcgt
      661 gcaccggccc cggtgcttgt ctggagcgtc ggcgccgctg ccgtgctcgt tgccgtcgct
      721 gcggtgtggt ctcgcagcgg ctggggcgcg gctgcctcca tcgcgctgct cggctccaca
      781 gccgttctct gggtcgcccg cgatccagca gcacaccctt cgaggccact tggcggatcc
      841 gtgatgccat gatcccccca accggcacga gtaccaatcg agcaacttcc cgcttgtggc
      901 tggttatccc cgcgctgctc ttcctgctct cgctggccat gaatctcaac gacgtgccca
      961 ccaccgagtt tcaccccgac gagacgcgct ggatcaaccg cgcgcactac ttccccgact
     1021 tgctcgaccc cttcgggccg acttgggagg atcaatacct gacccgcgga cagccgccgc
     1081 taggcagcta cctcatgggt ctcgggctgc tgctgcaggg ccgggatacc gaaacgaacg
     1141 gggtctggga cttcgcctac ggttccgaat ggaatcggct cagcggcgcg atgacggagg
     1201 aggctgacct gatcgccgcc cgtcgcacca acgccgtcat cgcggcgatc acggtggtag
     1261 tcgtgttctt tgtcggcagg gcgctgacgg ggacgatcgc tggaacggca ggtggcctgc
     1321 ttctggcagt ccacccactc catatctgga tcggcagcca ggctctctct gaccagttgc
     1381 tgatcctgat catcgctttg accgtgctga cggcgattca tctcggccgt ggccccagtc
     1441 gtggcaaagc gctgctgctg ggcatcctgc tcggccttgg cggggccgtc aagctttctc
     1501 ccctgctgct gagcatcccg gtcgcccttt acggcgcagc gctgctcctt ctggcgcgag
     1561 gcgggctcgt cgatcgggcg aaggcacgcc ggctggcacc attgctcctg gtgcagccca
     1621 tcatcgccgc gatcaccttc gtggccagct atccctatct gtggccagcg ccgatcgatc
     1681 gaacaatcaa cctgtttgag ctgcgcacgc aggagatgga ggggcaggcg tcggcgtggc
     1741 cacaggtcgc cattgcacac ccgggcgagg gcctcctgcg cgtgtacgac cggctcacgt
     1801 ggcagtccag cgcaaccggc aatcttgccg aggacatcct gcgccggttt ggcatcgaga
     1861 gcgacgtctg gggaatcgac ctgctgctcg ccgtgctcgg catcgccgcg ctcgcgtggc
     1921 tcgtgatccg ccgaggcctg acctcgggaa ccgccctcgc cgcctacgtc ctcgccgggc
     1981 agatcgcggg catcatcgtc ggcatgcgcg tcgattttta ccgctaccac ctgccgatcg
     2041 tgcttgcggt cgccatcctg gccggggtcg cggtgcaacg cgtgtgggct ctcctcgtgt
     2101 cccgcgcgac ccacgatcaa cgctccgtct cgggctcgac tggtcagccg atgatacaca
     2161 cagggagtcg atgatgccgc gaaaggtgag cttcaccccg ctcgcagcga tcctcgccgg
     2221 tctcgccatc ttcggcgcgg tggagacggt ccagttcgtg acggccgacc acgatggctg
     2281 ggacatcttc gcgccagtca gcgtcacgac cggaccgctc gttgtcgatc cacgaaacat
     2341 gcttgacgga gaggatcgag aagtgatcgc gcgcgtggtg cgcgagacac gtgactacgg
     2401 cgtgccatgg tcgatccagg tcatccgtcc acagggaagc atggggaacg tctccgcgca
     2461 ggaattcgcc gagcagcagt atgccgagca gccggtggag tccgtcgagg acgccgcaga
     2521 cggactgctc atgctggttt cggtacccga ggacccgaca gagacggaga tcgctttcgc
     2581 cgccggcccg aacttctacc ctcgtggagg cctcacaccg gagcggctgg cggagacggt
     2641 cggcacccag acggcgcaag caatcgagga gaagaggatc ggggacgcgg tgatcgaggc
     2701 agcgacatgg gtcgcgtgga tgcatctttt ccgcagcacc ccggaggaac ccgccaccca
     2761 tctcgaacgc ggcctgcagg aactgctgca gccactcgga gcggtcggat tcgccgcgct
     2821 ggcacttctc gttctcgccg ccgcagccgc aaccgtcgtg atgacccggc tgcatacgtc
     2881 gaccacggcc gtacccagca agctggacgg cgtgctggcg ggcgcgatcg cgcgggggcg
     2941 tgtcgaccgt ccggtactgg tcggcgccgt gctggacgct atcgatcgcg gtgtgctgac
     3001 catcactccg gacggcacgg tggcccccgg cccggtccag cccgcaaccg agcgtgaccg
     3061 actcatcgca gccacgctcg gcgaccgccc cgcccctatt cgcggcttgc tgcacaccct
     3121 tgaccgccgc gcccagctgg tgcggctgat ggaggacgtc ctcgccgctg ctggcgcgtt
     3181 tcatccgcga tcacgggttc ttacgctttg gctacggtgg atcgccgtcg cagggctgat
     3241 cgtcggcgtg ttggccatcg tcgtctcggt gctgggatcg gcacggtacg ccctcgtcgc
     3301 cgcgctcgcg cttgccgtca tctcgctcgt cgtcgcgatc tggaacgaaa ggcgctcgtg
     3361 ggcgacgcga actggcaagc gtgcagttgc gaaatggcgc gatctccatc aagacgggga
     3421 agaccgccag cggacgatgt tcgaaacgat caccgggatg gagtcactcg cagccacccg
     3481 aagtggggag gcgccagtgt cccctgccgc ctggcaagtc ttgcccgacg ttcattgaaa
     3541 ccggcgtcac gcaccgccga tacccggtcg gtgcattggt cgattccctg aagaggacca
     3601 ctgccgctcg ggcaacatgg gcattagcgg taccggcgcc acggcacgca ggagaaacac
     3661 ccgtcccggc gactaccgga acggatactg cacgtcggcg ttgccacatt gtcctgatca
     3721 gcgggctgcg acaagccctc gcatcctggc gtcgatttcc gcgccggatg ccgctggccc
     3781 gtagagcttc gtgacgtagt cgatcagcat tcggcgggtg gagaactgcg gcgcgattgt
     3841 gcagatcgca ttctttgcac gcgcaatcca ctcacgcggc aggccatcgg cgtcccgctg
     3901 gtagtagagc gggaccacct tacgctccag cgtctcgtaa atcgcgttcg cctcggcctc
     3961 gtcctgggcc tcgccttcca gtccagaggg gggaatgccc cagccattgc tctcgtccgc
     4021 ctcgtagccc tcgatccacc agccatcgag gatgctgcag ttcagcacgc cgttggcagc
     4081 cgccttcatc ccgctggtcc ccgaggcctc catagggtag cgtgggttgt tgagccatac
     4141 gtcgacaccg gcgacgagaa gactcgccag ccccatctcg tagtcctcgg caaagccgat
     4201 tcgcccctcg aaacgcgggt cacgcgcgcg ggtatagatc tcctggatca ggctcttgcc
     4261 gccctcgtcg gcggggtgcg ccttgccggc gaacacgagc tgcaccgggt tgtccgggtt
     4321 cgtgaggatc cgcgcgagac gatccatgtc ctggaagatc aacgtggcgc gcttgtaggt
     4381 cgcgaaccga cgggcgaacc cgatcgtcag cacgttggcg ttctggaacg atccggagga
     4441 gagcacctgc cacgcatcga actggccttc ggcgtaccgc cgccgcgcgc gctcgtccat
     4501 ttcgtcgatc agcgcctgct tggcgagctg gtgcgcctcc cagaactcct tgtccggaat
     4561 gttgcggatc gtctcccaga ccaggggatt ctcctgctga tttcgccaag tggacggcag
     4621 gtaactatca tacgtcggtc ggacaatggg agaaacccag gtccgaagat gcacaccgtt
     4681 ggtaatggag aagataggca cgtcatcgga atccgcatcg tcccacagct cctgccacat
     4741 cgagcgggag acctccccat gcttcttgct gacgccgttg acgtgcgcgg ccaagcgcat
     4801 ggagagcgcg gtgagattga agcccggctc atcagccata cgaccgagag cgaggaatgc
     4861 ctcgcggtcg acgcccaact cgccccagta ctggctgaag taccggtcca tcattgcgtc
     4921 cgagaagata tcgtgtcccg cggggaccgg cgtgtgcgtt gtgaagactg tcgaccgccg
     4981 aaccttgtcg atcgccactt cggccgtgtc gcctgcaacg acgtgctcac gcactcgctc
     5041 gaccatgtgc agtgccgcgt ggccctcgtt cgcatgccag taaacgggat tgatgccgag
     5101 cgcgcgcagt acgcgcacgc cgccgatgcc gagcacgatc tcctgacgca gacggtgctc
     5161 gcgatcgccg ccatagagtc gcgacgagag ctggcgggtc cactccgggt ttccttcaac
     5221 gtcggagtca agcaggtaga ttgacacccg gccgacacgc acctgccaga cagcgaggcg
     5281 caacggcgcc gtcccgtcgt ggagatcgac ctcaatgtgc aacggggtcc cgtcatcgtt
     5341 caggacctgc gttgtcggct cggaacccgg attgaggtcc gccggcacat cgtgctgcca
     5401 gccgtggtga tcgatgcgct ggcgcagata tccctggcga tagagcagcg agacgcccac
     5461 cagtgggatg cccatgtcac tcgcttcctt gacatggtca ccggcaagca cccccaggcc
     5521 tcctgagtag atcggcagcg cgcgatgcag accaaactcg gcggagaagt aggcgaccgg
     5581 cttgtccacg aggtcgggac gattgagccc cagccacgtc gtggaaacat cctcgctctg
     5641 catccggtcg aacgcggcga ggacacgatc gtatcgggca aggtacgccg ggttgttcgc
     5701 agccgcttca agtcgatgtc tgtcgatgcg gtggaggaag agcaccgggt tgtgctcgac
     5761 aacgtcccag agcaccgggt gcagttcttt gaacaggagt cgcgcagggt tgctccaggt
     5821 ccaccagagg ttgtaggcga tatcgttgag acgggagatc cgctcgggag gttccaccgt
     5881 gtaggacggt tggctcatga ccatcgttgg taccagctcc tcaggtattc gtatccatgc
     5941 caccccatcc cggccgggct tgtcactcgc tcagtgggat ggaggcaagg cacgcgtgca
     6001 gtatgccgca tcgctccatc atgaaccacc agccaagact ggcgcatcgt gacagggtac
     6061 actccgacag ccatccgcct gacgcagggg cagcacccag cgtgaacccg gactccacac
     6121 ccccaaccga ctccccgcca cggccgagca acagcagcag cctgctctgg atatcaggac
     6181 ttgcaatgct tgctggcgcc ctgctgttgc tcgtcgcatt tcccttcgac ggcaaccagg
     6241 acgcgctggt cgatctccgg ctgatgagcg acgccagcga acagggcttg ctggtctgga
     6301 ccgtggcgct cgtgctgctg accgggggct gggtcggcgg catactcgcc gctcgcggcc
     6361 gagcgctccg taaagtccgg ctcccggtga cggccttcac agtcctcgcc tccgtggcga
     6421 tgctgctcac ctaccccggt accgcgatcg atgtctatat ctacgcggcc cgctcgcatt
     6481 tgctcaccga tctcggcctt gatccctcga cgacactgcc cgaaacactg tgggacatcg
     6541 atccgtatgt ccggtatgca tcgaacgaat gggcgaacga cccttcccca tatggacccc
     6601 tttggaacct gatcgccgcc ccggcaactg cgttcgacga tgagaacatc gccgtggcgg
     6661 tggtgatctt caaggcgatc atggcggccg gtgtcgccat cacgggctgg ctgatccacg
     6721 atatcgcgcg gcgcgtccag cccggactcg cagcgtcggc caccatcggc tggctgtgga
     6781 gcccagtggt gctttgggag ggcatcgcca acgggcacaa cgacgtgctg cttgccctgc
     6841 tgctcgtcac cgcgctctgg tgttggtatc ggggatatct gggatcgctc ctcccccttc
     6901 tcggtgccgc ctccctgctc aaggttgtcg cgatgatcac catcccggct gctgccgtcg
     6961 cgatcatccg gcgaaccggc tggaaccggg cgctggcagc cattgcaggt cgggcagccc
     7021 tattctcgct cctgatcgcg tgggttgctt tcgcaccctt ctacgacgtc gccggcgtca
     7081 tcgacgccat ccgcgcccaa accacggtca tcgtcacctc cccggccctg ctggcgcagg
     7141 cgatcagcaa cgagctcggc tggggactcg acgtcggcga ggactttccg accttcggcg
     7201 tgccaatgat catcctgctg acgctggccg gcgcgatcgc tgcctggctg cggccatcac
     7261 agtttccgac gatcgcctac gagcagctct tctggtttct ggtactggcg acgtcgaacc
     7321 tccgaccgtg gtatacgatc tggctggtcg cgctcggatt ggcgctgccg ctcggcatgc
     7381 cattcgcgcg ggcggcagcg ttcgcgattg gcgggctcgg cacgtacgcc tacctgctat
     7441 gggtccggca ctggtacgcg gcggaatggc tcgtcgaggt gggaatcacc gtcgggatca
     7501 tgctgggccc cacactgctg gtgacactct gggcagcttt cagcccactg caccggcggc
     7561 tccggcaagc gcacgccgtg cccaccgagg cctaggcccc tgcctggccc accacactcg
     7621 ctgcaagcac ctattcctcg ccgaggtagg ccttgcgcac accctcatcg cgcatgagca
     7681 catgagcgtc gtccgacagc acgatctcgc cggtctgcat cacgtaccct cggtgcgcga
     7741 tcgccagcga catcagcgcg ttctgctcga cgagcagcac cgtggttcct tgggcattga
     7801 tgtcctgcac aattcggaag atctgctcca cgaggatcgg ggcgagcccc atcgacggct
     7861 cgtcgaggag cagcacgcgc ggccgcgcca tcagcgcccg accgatggcc agcatctgct
     7921 gctcgccacc ggagagcgta cccgccttct g
//
LOCUS       DASYWL010000341         3702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_449312, whole genome shotgun sequence.
ACCESSION   DASYWL010000341 DASYWL010000000
VERSION     DASYWL010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449312"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VGR22_11990"
     CDS             complement(<1..366)
                     /locus_tag="VGR22_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129328.1"
                     /translation="MTSPISSAITDPTEGKTVAEHPTDDTAHLLELAEEAGTAIDTVV
                     VLDFGSQYSQLITRRVRECGVYCELMHHDTAWDEVSHLDPKGIILSGGPASVYADDAP
                     TLPAWVLERELPVLGICYGM"
     gene            complement(464..1369)
                     /locus_tag="VGR22_11995"
     CDS             complement(464..1369)
                     /locus_tag="VGR22_11995"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEV2129329.1"
                     /translation="MTVTDPFVVIAERITTGARLVRHWPLEGGVSASIDALEVALPDG
                     TLQRVVVRRHGATDRTANPDVARDEYRLLTVLHAAGLPVPTPRLLTDTDDPFPEPCIV
                     VDFVEGTTGVPPHRAHIAARRMADFLCALHAIDLRESGLVFLPSLEQRVERSLRQPLA
                     NAESESIRQPLADRWPQATTSTTAVLHGDFWPGNILWRDGTIAAVIDWEDAVIGDPLA
                     DLAKCRLELLWEFGEQVMDTFTDHYVAISSVATANLPFWELHSTLGPIEKLGTWGLDP
                     EDEARMREKLHTYAERARATLLEER"
     gene            complement(1366..1776)
                     /locus_tag="VGR22_12000"
     CDS             complement(1366..1776)
                     /locus_tag="VGR22_12000"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV2129330.1"
                     /translation="MTSGRPSIRSVVVHFLPALSVMAVIFTFSDQPKLPQIPSLSTQI
                     TSVAGHFTVYLVLALLLWWALGVFDLSTRQRYAIAFVIATLYGISDEWHQSFVPGRQP
                     DVLDVLTDAIGVTTGLLAIHLAHRSERLRALVPR"
     gene            complement(1842..2297)
                     /locus_tag="VGR22_12005"
     CDS             complement(1842..2297)
                     /locus_tag="VGR22_12005"
                     /inference="COORDINATES: protein motif:HMM:NF017663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEV2129331.1"
                     /translation="MSDAPVTVRLTLPLPPGVNNQYVTAGNRRVLSKSAKEFKKEVER
                     LLLDHREKNRITTAQERTVKESFIGVYMTFFFETPLKRDLDGGLKIALDAIASGLGFD
                     DRKVVDIHLTKRIDPLRPRLEIEMETITDWQFDESYVYLGDTEDDASAQ"
     gene            complement(2449..>3702)
                     /locus_tag="VGR22_12010"
     CDS             complement(2449..>3702)
                     /locus_tag="VGR22_12010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEV2129332.1"
                     /translation="RRRPGRRGPARGPGMTPPPNVEPIEVTVERIVGDGKGIAFHDGQ
                     TVFIPETAPGDRIRAKVRSHRGKVIQAEIMEILEPSPQRVEPPCPYFFRCGGCDFQQF
                     SYEDQLATKASMIEDSLRRIAKLDPVPEVPITPSPQPLEYRSRAEWQIDQKERKIGYF
                     ASGTHDVIDVEICPILTPNLKTLLTTLREDFANGLVSREAYEYRGVEGDVGLTLEPTG
                     ATRSQLVMRTVGGHLYRYNAETFFQANIPVTELLLERVLEIADVAKDEPGIAIDLYCG
                     VGLFTRPLAVRFPRVIGVESDKTTTDFAAENTEAPEDGSNVRIVNAPVERWIAEDRSP
                     LGRVALVVFDPPRAGAGPKVIEDMLRLRPAHIAAVSCDPATFARDIRGLIDGGYELVS
                     IEAFDMFPQTHHVELIGHLHRAELS"
BASE COUNT          622 a         1130 c         1277 g          673 t
ORIGIN      
        1 catgccgtag cagatgccga ggaccggtag ctcacgttcc agcacccagg cgggcagggt
       61 gggcgcgtcg tcggcgtaga cgctcgccgg accgccgctg aggatgatgc ctttcgggtc
      121 gaggtggctg acctcgtccc atgcggtgtc gtggtgcatc agctcacagt agacgccgca
      181 ctcgcgcacg cgccgagtga tgagctggct gtactgcgag ccgaaatcaa gcaccaccac
      241 cgtgtcgatg gcggtgccgg cctcctcggc cagctcgagc aggtgcgcgg tgtcgtcggt
      301 cgggtgctcg gcgacggtct tgccctcggt cgggtcagtg atagcggacg agatcgggga
      361 ggtcacgcag gttttctccg atggggttgg atgggttccg gtccgggtgg tagcacgccg
      421 cggcagcgtc gctgggatca gtttacccga cggcaaactc cgctcatcgc tcctccaaca
      481 gggtggcgcg ggcacgctca gcgtaggtgt gcagtttctc gcgcattctg gcctcgtcct
      541 ccggatcaag cccccaggtg ccgagttttt cgatgggtcc gagcgtggaa tgaagctccc
      601 agaagggcag gttggcggta gcaacgctgc ttatcgccac gtagtgatcg gtaaaggtat
      661 ccatcacctg ttcgccgaat tcccagagca attcgagtcg gcatttggcc aggtcagcga
      721 gcgggtctcc gatcactgca tcctcccagt cgatcacggc ggcgatcgta ccgtcccgcc
      781 agaggatatt ccccggccag aaatcgccgt gcagcactgc tgtggtgctg gtggtcgctt
      841 gaggccagcg gtcggcgaga ggttgccgaa tcgactcgga ttccgcgttt gcgagcggct
      901 gccgcaggga tcgctcgaca cgttgctcaa ggcttggcag gaatacgagt ccactctctc
      961 gcaagtcgat cgcgtgcaat gcacacagaa agtcggccat ccgccgggcg gcaatgtgcg
     1021 ctcgatgtgg cggcacgcca gtcgtgccct cgacgaagtc gaccacgatg cacggttccg
     1081 ggaagggatc gtcagtgtcg gtgaggaggc gtggcgtggg gaccggcagc ccggcagcgt
     1141 gcagcacggt caacaggcgg tattcgtctc gtgcgacatc agggttggcg gtgcgatcag
     1201 ttgcaccgtg ccgacgcacg accactcgct gcaacgtccc atccggcaga gcgacttcca
     1261 aggcgtcgat gctcgccgat acgccgccct ccagaggcca gtggcgaacg agccgtgcac
     1321 ctgtggtgat gcgttcggcg atgacgacga acgggtccgt gacggtcacc ggggcaccag
     1381 tgcgcgcagg cgctcggatc ggtgcgccag gtggatggcg aggagaccgg ttgtcacgcc
     1441 gatggcatcc gtgagcacgt cgagcacgtc cggttggcga cctggaacaa agctctggtg
     1501 ccactcgtct gagatgccgt agagcgtggc gatgacgaag gcgatcgcgt accgctggcg
     1561 tgtggacagg tcgaatacgc ccagcgccca ccagagcagg agcgcgagca cgaggtagac
     1621 ggtgaagtgg ccggcgactg aggtgatctg cgtggagagc gagggaatct gcggcagctt
     1681 cggctggtcg gagaaggtaa agatcacggc catcacggag agcgcgggca ggaagtggac
     1741 aaccacggaa cgtatggagg ggcggcctga agtcatgggg cggcagtggt cctcacatct
     1801 ggagcgccat cacaacgcct aacgatacac ccaaggcgtg cttactgcgc cgatgcgtca
     1861 tcctcggtat cgccgaggta gacgtaggac tcgtcgaact gccagtcggt aatcgtctcc
     1921 atctcgatct ccaaccgtgg acggagcgga tcgatccgtt tggtgaggtg gatatcgacc
     1981 accttccggt cgtcgaagcc gagcccgctg gcaatcgcgt ccagcgcgat cttcaggcca
     2041 ccgtccaggt ctcgcttgag tggcgtctca aagaagaagg tcatgtagac gccgatgaag
     2101 gattccttga ccgttcgttc ctgagccgtg gtgatccggt tcttctcccg atgatcgagc
     2161 agtagccgct cgacctcttt cttgaactcc ttcgcactct tgctgagcac ccggcggttg
     2221 ccggcagtga catactggtt gttgacgccg ggcggaagcg gcagcgtcag gcggacggtg
     2281 accggggcgt cgctcatcac ggcatagcga tggccggcac aaggccggcg ctacgtggac
     2341 gtagcgtgac cgtgattgat gtcatcacga agcggcgacc gggcggaaac cctgcgggct
     2401 ccgcccctat ccgccctgtg ggcgcctgct catggcgagg tggtgccatc acgacagctc
     2461 ggcccgatgc aggtggccga tcagctcgac gtggtgggtc tgcgggaaca tgtcgaatgc
     2521 ctcgatcgag accagctcgt agccgccgtc gatcagcccg cggatgtccc gtgcgaaggt
     2581 cgccgggtcg caggagacgg ccgcgatgtg cgctggcctg agccggagca tgtcctcgat
     2641 gaccttcggg ccggcaccgg ctcgcggcgg gtcgaagacg accagtgcga cgcggccgag
     2701 tggcgatcgg tcctcggcga tccatctctc gaccggtgcg ttgacgatgc ggacgttgct
     2761 cccgtcctcc ggcgcctcgg tgttctcggc ggcgaagtcg gtggtcgttt tgtcgctctc
     2821 aacgccgatc acgcggggga agcgaaccgc cagcggcctg gtgaagagac cgactccgca
     2881 gtagagatcg atcgcgatgc ccggctcgtc cttcgcgaca tcggcgatct ccagcacgcg
     2941 ctccagcagg agttcggtga ccgggatgtt cgcctggaag aatgtctcgg cgttgtagcg
     3001 gtagagatgc ccaccgacgg tgcgcatcac cagctgcgag cgggtcgcgc cagtcggctc
     3061 cagcgtcaag ccgacatctc cctcgacccc gcggtactca tacgcctcgc ggctgaccag
     3121 cccgttggcg aagtcctctc ggagcgtggt cagcagggtt ttcaggttcg gtgtgaggat
     3181 tgggcagatc tcgacatcga tgacatcgtg cgttccgctc gcgaagtagc cgatcttgcg
     3241 ctccttctgg tcgatctgcc actccgcacg ggagcggtac tccagtggct gcggcgaggg
     3301 cgtgatcggc acctccggca cggggtcgag cttggcgatg cgtcgcaggc tgtcctcgat
     3361 catggatgcc ttggttgcca gctggtcctc ataggagaac tgctggaagt cgcagccgcc
     3421 gcaccggaag aagtagggac aggggggctc gacgcgctgc ggcgacggtt cgaggatctc
     3481 catgatctcg gcctgtatca ccttgccccg atggcttcgc accttcgcgc ggatacggtc
     3541 acccggggcc gtctccggga tgaagacggt ttgcccatcg tggaaggcga tgcccttgcc
     3601 gtcgccgacg atgcgctcga ccgtcacctc gatcggctcg acgttcggcg gtggggtcat
     3661 gccgggaccg cgcgccgggc cacgccggcc agggcgccgt cg
//
LOCUS       DASYWL010000342         9897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_524632, whole genome shotgun sequence.
ACCESSION   DASYWL010000342 DASYWL010000000
VERSION     DASYWL010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9897
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524632"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(218..1195)
                     /gene="meaB"
                     /locus_tag="VGR22_12015"
     CDS             complement(218..1195)
                     /gene="meaB"
                     /locus_tag="VGR22_12015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEV2129333.1"
                     /translation="MCVMRRLSSSEQLVKGVLAGSPAAVARAITLIENDQPGGREVLD
                     HVFPQTGHAHIVGVTGPPGSGKSSLVNEIIRQSRAVGKKIAVLAIDPSSPITGGATLG
                     DRIRMLEWHADPGVFVRSMASRRFGSGLAQHTLTVAHVFDAAGFDPIIIETVGTGQDE
                     VAIAALAHTTVLLQVPGSGDSVQTLKAGSLEIGDLIVVSKGDRTDALTLARDLRRLQT
                     LREDVGQQTSGWTPRVITTSSIERSGIDTLIAGLGEHREWLRESDEWDRRVRTMLGTE
                     IASRVQLRLLDEVVGGSAAQDEVIDQVLARELSPLTAADRLLEQLRSGQ"
     gene            complement(1186..1635)
                     /locus_tag="VGR22_12020"
     CDS             complement(1186..1635)
                     /locus_tag="VGR22_12020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEV2129334.1"
                     /translation="MTDRTDSPTPSEDQPVRVLVAKPGLDGHDRGAKVMARAFRDAGF
                     EVVYTGLFQTPEMIANTALQEDVDVVGLSILSGAHMSLFPPIMEALRQVGRDDALVIA
                     GGTIPSGDIPKIREMGVAAVFGPGTRLQNAIAFVREHTGRANPAECA"
     gene            complement(1669..2535)
                     /locus_tag="VGR22_12025"
     CDS             complement(1669..2535)
                     /locus_tag="VGR22_12025"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEV2129335.1"
                     /translation="MTQGNRIGIIGAGTMGAGIAQVAAASGFEVALIDTKDEFVASGL
                     QRIESGLQRLVGRERMSGEERDATLGRITTGTSMETLAGSELVIEAVVESFDTKVEVL
                     RQVADVVDKTTILATNTSSISITALAATVPNPERFAGMHFFNPVPVLKLVEVVRGLQT
                     SDATAATIREVAEQMGKTPAEVADMPGFAANRILIPMINEAIFAVAEGVASAEEIDMV
                     MTLGASHPLGPLALADLIGLDVCLHIMEVLHKDFGDDKYRPAPLLRQMVSAGRLGRKS
                     GQGFHSYENPQS"
     gene            complement(2583..3263)
                     /gene="pth"
                     /locus_tag="VGR22_12030"
     CDS             complement(2583..3263)
                     /gene="pth"
                     /locus_tag="VGR22_12030"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEV2129336.1"
                     /translation="MLQRLKQLLRPGMGPDTEDDRDMATGAHIIIGLGNPGLKYANTR
                     HNVGFMVMEELARRSDGGGSRKRFRSQVLDARLPSGRSILVQPQTYMNESGHAVREVR
                     NWYRADPERILIVVDDLDLPFGQLRLRQRGGAGGHNGLKSIIEQLDTQEFPRLRVGIG
                     RGPNQAKAHVLSNFAPSEQQHLPIVIDAAAGAVEFWMREGIVTAMNEVNGRASVLPND
                     QLATGTSV"
     gene            3383..4150
                     /locus_tag="VGR22_12035"
     CDS             3383..4150
                     /locus_tag="VGR22_12035"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEV2129337.1"
                     /translation="MFGIGPMELTIIAVVALLIFGPEKLPEMMGQAGKLVRDFRNMTA
                     EMSGEFEKTIAEAKGISKDFQGEVGSMRNQVNSVTKSVQKDLGGGKSTRTSGKKASST
                     SKRSTSGSSSSKSYSGSKATTTRKSGSDKSSSASKGSSSSSSASKSTSPKKADTATTK
                     PATPQTATREDPTAAISLFEPAEPRRQRRARKATLSAFGEATPRIASINGEETASDLA
                     ASISDNGETATRMVDLNNPLVRARQRRQSAGYARKIS"
     gene            4156..5403
                     /gene="tgt"
                     /locus_tag="VGR22_12040"
     CDS             4156..5403
                     /gene="tgt"
                     /locus_tag="VGR22_12040"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEV2129338.1"
                     /translation="MVEGKLTAGSANVLAYSEANGGRFRFDLLSEDRATWARRGRITT
                     ARGEIETPVFMPVGTQATVKTLLPEEVESTGAQIILSNTYHLMLRPGGELIREAGGLH
                     RFMQWHRPILTDSGGFQVFSLAASNKVTEEGVRFNSHIDGTRWTMTPESATDLQLAFG
                     SDIMMQLDHVIGLPADRTKIQDATERSSRWLERSIAALHDRQPDESPAVLFGIQQGGM
                     EADLRREHAERLGTMDVAGCAVGGLSVGEPKAIMAEMLEHSTPFLPREKPRYLMGVGS
                     PEDLWHGVARGVDMFDCVLPTRAARNSSMYTPDGRITVSNAAWRHTHRPVDETCDCRA
                     CTQYTAAYLHHLFKANEVLGLRLASEHNLRFLARIMEEIRAAIKEGTFGERAEAFLSR
                     YKAVKMHDVVALPSRAPMVSEGE"
     gene            5405..5911
                     /gene="acpS"
                     /locus_tag="VGR22_12045"
     CDS             5405..5911
                     /gene="acpS"
                     /locus_tag="VGR22_12045"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEV2129339.1"
                     /translation="MGERRRRRWPWREPELEQREGPGWYHPIFDPESEGAIAVGTDII
                     EVARIERSYRDFGERFLHRVFTERERERYRGRPNELAARFAAKEATSKALGTGIIGIR
                     WREMEVLPNRRGKPVLILHGQAAERARQLGLNHFSVSLTHSRTDAMAFVIGMRGPEPS
                     TSNEDEHK"
     gene            5911..6927
                     /locus_tag="VGR22_12050"
     CDS             5911..6927
                     /locus_tag="VGR22_12050"
                     /inference="COORDINATES: protein motif:HMM:NF013425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP/ATP-dependent (S)-NAD(P)H-hydrate
                     dehydratase"
                     /protein_id="HEV2129340.1"
                     /translation="MTEHTRPELSGRHQHWADSDLAKSIAPRRSFGTHKWEVGGMVVI
                     AGSPSYIGAAQLCSRSAGRSGAGIVYLAAPRQVINVIAAAIPEVAHIPLGSTDTLAGV
                     RHALGELGDSLRRARAVVLGPGVTTDEGASTLLAALFGVSDHGRNSPMSIGFAASQAV
                     KEETESSEALLFANLDATVVVDADGLNWLAKQDNWWTKVPEGRMILTPHPGEMARLLR
                     LDDPDEVIADPIAMVERAAKEWKQTVILKYGHTAASNGERTVVAEDAPVSLATAGSGD
                     VFAGMVGAFAAQGLAPIDAAALALHIGPRAARRGEQRFGAFGLIAIDLPDAIALELAT
                     LTDE"
     gene            6957..7475
                     /locus_tag="VGR22_12055"
     CDS             6957..7475
                     /locus_tag="VGR22_12055"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV2129341.1"
                     /translation="MTDETQTGVDPEPIVREIMQTNVPTVAPEDTVPVVATMMVQSGI
                     NGVPVIENGRMIGIITDSDIIAREADVDAPMPVVFLDAYFTADAGRSFEEEARRALAI
                     NARQLMSSPVVSIRETATLSHIATLMIDEGVNPVPVLDASDNLVGIVSRADLVRVIAC
                     LESEGTTDDTTA"
     gene            7459..8742
                     /gene="alr"
                     /locus_tag="VGR22_12060"
     CDS             7459..8742
                     /gene="alr"
                     /locus_tag="VGR22_12060"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEV2129342.1"
                     /translation="MTQRRNVDALLSEWDGRPSMALVVLDQLSTNVQALRHRVGSDTR
                     FMAVVKAEGYGHGAVPIAKTAVRAGADEVAVATVEEGVRLRRGGVTAPILVLGPIGTK
                     ERPRAIAFDLMLVIGDPDFVRGLAADVRKTGRKTPLDVHLKVDTGMHRFGVCPERAVG
                     VAKVIDAVPELRLAGVMTHFASADDPDAASTHMQAAMFDEAVAAIRAAGIEVSSEHLC
                     NSAATLRYPEYHRDRVRVGISMYGLRPDPELVPVPDPIRPIMSVHSRITRIEELQPGE
                     RVSYGGTWQAEAPSHVALVPIGYADGYSRQGSNRAWVDVRGQRSPVRGRVCMDQTMVE
                     VGPDARSGDIVTVIGDGQHGEAPSIDELAVLWGTISHEVATGFAARRLGHLYLQGGRL
                     VAVADLWDYREIDEDAFAQPEIGEQDTTGVAQEAR"
     gene            complement(8975..9508)
                     /locus_tag="VGR22_12065"
     CDS             complement(8975..9508)
                     /locus_tag="VGR22_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV2129343.1"
                     /translation="MDSESRDYQRVMDNALQLVYSHYHRLATQLSPEAFRQLTLDQLR
                     SGPLGQDLLRLARLARGQTRESQDVVLEAIDSVLQLLFWPVAADDYTVPRTFWDTDLG
                     RMLALAKFRAYESSELVSIGNAAQQLGVTRPTIYRWMDEKMLNYVRDDMSGRTFVVRK
                     DIERRKQIEMELGGGEF"
BASE COUNT         1748 a         3179 c         3254 g         1716 t
ORIGIN      
        1 tgatggagga ggcgatgggg cgccggccgc caccgaccat cgacgattgg tgacctcgcc
       61 tgctccttga aatccctaca tcgcgcgggc accgatcgaa agactcagtc agatgagcag
      121 gtggtgctca cccttcctga agcctatgcc aggcggtaat agttcgaccc gctttagcgc
      181 tgccgcagtg ctggtgtaac agtcacgatg gctcgcatca ctggcccgat cgcagttgct
      241 ccagtagccg gtcggcggcg gtgaggggac tcaactcgcg cgcgagcacc tggtcgatca
      301 cctcgtcctg cgcggcggag ccgccgacca cctcgtccag caatcgcagc tgaacccgcg
      361 acgcgatctc cgtgccaagc atggtcctta cccggcgatc ccactcgtcg ctctcgcgaa
      421 gccactcgcg gtgctcccca agcccagcga tcagcgtgtc gattcccgac cgctcgatcg
      481 agctcgtggt gatcaccctc ggcgtccagc cgctcgtctg ctgtccgacg tcctctcgca
      541 gggtctggag gcgccggagg tcgcgggcga gcgtcagcgc gtcagtacgg tcacccttgg
      601 agacgacgat gaggtcgccg atctccagcg agcccgcctt gagggtctgc acgctgtccc
      661 cgctacccgg tacctgcagt agcaccgtcg tgtgggcgag cgcggcgatg gcgacctcat
      721 cctgaccggt gccaaccgtc tcgatgatga tcgggtcgaa ccccgctgcg tcgaagacgt
      781 gcgccacggt cagggtgtgc tgtgccaatc cgctgccgaa tcgccgcgac gccatgctgc
      841 ggacaaagac gcccgggtcg gcgtgccact ccagcatgcg gatacgatcg ccgagggtcg
      901 cgccgcccgt gatcggcgag gaaggatcga tcgcaagcac ggcgatcttc ttccccaccg
      961 ctcgactctg ccggatgatc tcgttaacga gcgacgactt gccggatccc ggcgggcccg
     1021 tcacaccgac gatgtgcgca tgtcctgttt gcggaaagac gtgatcgagc acctcacgtc
     1081 caccgggctg atcgttctcg atcaatgtta tcgcacgggc aacagcagcc ggactgccgg
     1141 cgagtacccc tttgacaagc tgctccgatg agctcagtcg tcgcatcacg cacattccgc
     1201 cgggttggcc ctgccggtat gctctcgaac gaaggcgatg gcattctgca ggcgcgtgcc
     1261 ggggccgaac accgctgcga ctcccatctc ccggatcttc ggaatatcgc ccgacggaat
     1321 ggtcccgccc gcgatgacga gcgcgtcgtc acgacccacc tgcctcagcg cctccatgat
     1381 tggtgggaag agcgacatgt gagcacccga gagaatgctg aggccgacga cgtcgacgtc
     1441 ctcctgaagc gcggtgttgg cgatcatctc cggcgtctgg aagagccccg tgtagacgac
     1501 ctcgaagccg gcatcgcgga aggctcgcgc catcaccttg gcgccgcggt cgtgcccgtc
     1561 aaggcccggc ttggcgacca gcacgcgtac cggttggtcc tccgaagggg tgggggaatc
     1621 agttcgatca gtcatcgcgc tcctcgaatt gtgagacagg ccgaaaggtc agctctgcgg
     1681 gttctcgtag ctgtgaaacc cctgaccgct ctttcggccg aggcgtccgg cggagaccat
     1741 ctggcgcagc agcggcgcgg ggcgatactt gtcgtcgccg aagtccttgt gcagcacttc
     1801 catgatatgg aggcagacat cgagaccaat caggtcggcg agcgccagcg gaccgagtgg
     1861 gtgcgacgcg cccagcgtca tcaccatgtc gatctcctcg gcgctggcga cgccctcggc
     1921 caccgcgaag atcgcttcgt tgatcattgg gatgaggatc cggttggccg cgaaaccggg
     1981 catgtcggca acctcggccg gggtcttgcc catctgctct gctacctcgc ggatcgtcgc
     2041 ggcggttgcg tccgatgtct gcaggccccg cacgacctcg accagtttca gcactggaac
     2101 ggggttgaaa aagtgcatcc cggcgaaacg ttcgggattc ggcaccgtgg cagcaagcgc
     2161 ggtgatcgag atcgaggagg tattcgtcgc gagaatcgtg gtcttatcga cgacatccgc
     2221 gacctgccga agaacctcca ccttcgtgtc gaacgactcc acgaccgctt cgatcaccag
     2281 ctcgctcccg gcgagcgtct ccatcgacgt ccccgtggtg atccgaccaa gggtggcatc
     2341 gcgctcttca ccggacatcc gctccctgcc aacgaggcgc tgcaggcccg actcgatcct
     2401 ctgcagaccg gaagcaacga actcgtcctt cgtgtcgatc aatgcgacct caaagccgct
     2461 cgctgcggcg acctgggcga tgcctgcgcc catggtgccc gcgccgatga tgccgattct
     2521 gttgccttgt gtcatgcgtg gtcgtgtcct cgtgtgcagc gcgtcaggcc ggtgtcccgg
     2581 cattatacgg aggttcccgt ggcgagctgg tcgttcggca ggacggatgc gcgcccgttg
     2641 acctcgttca tcgccgtgac gatgccctcg cgcatccaaa actccaccgc accggcagcg
     2701 gcatcgatca caatcggcag atgctgttgc tcgctcggcg cgaagttcga taggacgtgg
     2761 gccttcgcct gattcggccc ccggccaatt ccgacgcgca gcctcgggaa ctcctgcgtg
     2821 tcgagctgct caatgatcga cttgagccca ttgtgacctc cggcaccgcc gcgctgacgg
     2881 aggcggagct ggccgaaggg caggtcgaga tcgtccacca cgatcaggat ccgctcggga
     2941 tcggcgcggt accagttgcg cacctcgcga acggcgtgac cgctctcgtt catgtaggtc
     3001 tgtggctgaa cgaggatgga ccttccgctt gggaggcggg catcgagaac ttgcgaccga
     3061 aaccgcttgc gggagccgcc cccgtcgctt cgccgcgcca gttcttccat caccatgaac
     3121 ccgacattgt gccgggtatt ggcatacttg agtcccgggt ttcctaggcc gataatgata
     3181 tgcgcgccgg ttgccatgtc tcgatcgtcc tcggtatccg gccccatacc cgggcgcagg
     3241 agctgtttga gtcgttgcag catggaggcg tcctccactg ggctatactt tccagcgaac
     3301 cattgtgcca tgaccccgcc agcacggctg cgacttgggt gcgggcgacg gtacactgac
     3361 gacttgtgac gaaaggttgg cgatgtttgg tattggaccg atggagctca ccatcatcgc
     3421 ggtggtggcg ctgctcatct tcggacccga gaagctgccc gagatgatgg ggcaggccgg
     3481 caagctcgtg cgtgatttcc ggaacatgac cgccgagatg tcgggggagt tcgaaaagac
     3541 catcgccgaa gccaagggga tcagcaagga tttccagggc gaggtcggct cgatgcgaaa
     3601 ccaggtcaac tcggtgacca agagtgtcca gaaggacctc ggtggcggca agtcgacccg
     3661 cacatccggc aagaaggcct cgagcacgtc gaagcggtcg acgtccggca gttcgtcgtc
     3721 gaagagctat tcggggtcga aggccaccac gacgcgcaag tctggctcgg acaagagttc
     3781 cagcgcatcg aagggctcgt cgtcctcgtc gagcgcatcg aagtcgacgt cacccaagaa
     3841 ggccgacacg gccacgacca aacccgcaac accacagaca gccacgcgcg aggatccgac
     3901 cgcggcaatc tcgctctttg agccagcgga gccgcgccgc cagcggcgag ctcggaaggc
     3961 gaccctctcg gcgttcggtg aggcaacccc acgcatcgct tccatcaacg gcgaggagac
     4021 ggccagtgac ctggccgcgt cgatctctga caacggcgag acagcgacga ggatggtcga
     4081 tctcaacaac ccgctcgttc gcgcccggca gcgccgacag tccgccggat acgcccgcaa
     4141 gatttcctga gcggcatggt cgaaggcaag ctgacagccg ggagtgccaa tgtgctcgcc
     4201 tacagcgagg cgaacggcgg ccggttccgc ttcgatctgc tctctgaaga cagggcgacc
     4261 tgggcccgcc gagggcgcat taccactgcg cgcggggaga tcgagacgcc ggttttcatg
     4321 ccggtcggca cccaggcaac ggtgaagact ctcttgccgg aggaggtcga gtccaccggc
     4381 gcgcagatca tcctctccaa cacctatcac ctcatgttgc ggcctggcgg cgagctgatc
     4441 cgcgaggcgg gtggtctaca ccggttcatg cagtggcacc ggccgatcct caccgacagc
     4501 ggcgggttcc aggtcttttc cctggctgcc agcaacaagg tgaccgagga gggcgtgcgc
     4561 ttcaactcgc acattgacgg gacacgctgg acgatgacgc cagagagcgc gaccgacctg
     4621 cagcttgcct tcgggtcgga catcatgatg cagctcgacc acgtgatcgg gctccccgca
     4681 gatcgcacga agatccagga cgccaccgag cggtcatccc gctggctgga acgctccatc
     4741 gcggcgctgc acgatcggca gcccgacgaa tctcccgctg tcctcttcgg catccagcag
     4801 ggtggcatgg aggccgatct tcgccgagag catgccgagc ggctcggaac catggacgtg
     4861 gccggctgcg cggtgggcgg gctcagcgtc ggcgaaccga aggccatcat ggcggagatg
     4921 ctggagcatt cgactccatt cctgccgcga gagaagccac gctacctgat gggggtcggc
     4981 tcgccggagg acctctggca tggcgtcgca cgcggggtcg acatgttcga ctgcgtactg
     5041 cccacgcgtg cggcgcgcaa ctccagcatg tacaccccgg acggacgtat tacggtctcc
     5101 aacgccgcgt ggcggcatac ccatcggccg gtcgacgaaa cgtgcgactg ccgcgcctgc
     5161 acgcagtaca ccgccgccta cctgcaccat ctcttcaagg ccaacgaggt gctggggctg
     5221 cggcttgcct cggagcacaa cctgcgcttt ctcgcccgca tcatggagga gatccgtgcg
     5281 gcgatcaagg aaggcacatt tggcgagcgc gccgaggcgt tcctgtcgcg ctacaaggcc
     5341 gtgaagatgc acgacgtcgt ggcgttgccg agccgggcgc cgatggtgag cgagggggag
     5401 tgagatgggt gagcgacgac ggcggcgatg gccgtggcgc gagccggagc tggagcagcg
     5461 cgagggaccg ggctggtacc atcccatctt cgatccggag agcgaggggg cgatcgccgt
     5521 cggcacggac atcatcgagg tcgcgcgcat cgagcggtcg taccgcgatt tcggcgagcg
     5581 attcctgcac cgcgtcttca cggagcgcga gcgcgagcgg tatcggggac gccccaatga
     5641 gctggcggca agattcgccg ccaaggaggc aacctccaag gcgctgggca ccggcatcat
     5701 tggcatccgg tggcgggaga tggaggtgct gccgaaccga cgcggcaagc ccgtgctgat
     5761 cctgcatggg caggccgccg agcgggcgag acaactcggc ttgaaccatt tcagcgtttc
     5821 cctgacgcac tcgcgcaccg atgcgatggc cttcgtgatc ggcatgcgcg gccctgaacc
     5881 atcgaccagc aacgaggatg agcacaagta atgacggaac atacccggcc ggagctgagc
     5941 ggacggcacc agcattgggc ggacagcgac ctcgccaagt cgatcgctcc gcggcgctct
     6001 ttcggcacgc acaagtggga agtcggcggc atggtggtga tcgcgggatc gccgtcctac
     6061 atcggtgcgg cgcagctctg tagccgctcg gccgggcggt ccggcgccgg catcgtctat
     6121 ctcgctgcac cccgccaggt gatcaatgtc atcgctgcgg cgattccgga ggtggcacac
     6181 attccgctcg gatcgaccga cacgctcgcc ggtgtccgcc acgccttggg cgagctcggt
     6241 gactcgctcc ggcgggcgcg tgccgtggtg ctcggtcccg gtgtgaccac cgacgaggga
     6301 gcctcgacac tcctggctgc gcttttcggc gtttccgacc acgggcgcaa ctcgccgatg
     6361 agcatcggct tcgcggcgtc tcaagccgtc aaggaggaaa ccgaaagctc cgaggcgttg
     6421 ctcttcgcca acttggacgc gaccgtcgtg gttgatgccg acggcctcaa ctggctcgcg
     6481 aagcaggaca actggtggac caaggtgccc gagggcagga tgatcctgac cccgcatccg
     6541 ggcgagatgg cgcgactgct ccgcctcgac gatcctgacg aggtgatcgc agacccgatc
     6601 gcgatggtcg agagggccgc gaaggagtgg aagcagacgg tgatcctgaa gtacggccac
     6661 actgccgctt ccaacggcga gcggacggtc gtcgcggagg atgcgccggt ttcgctcgcg
     6721 actgccggca gcggcgatgt cttcgccggc atggttgggg cgttcgccgc gcagggactc
     6781 gcgcccatcg acgcagcagc cctggcactc catatcggac cgcgtgcggc tcggcgtggt
     6841 gagcagcgct tcggtgcctt cggattgatt gcgatcgacc taccagacgc catcgcgctc
     6901 gagttggcga ccctcaccga cgagtaggcc gcaacgaggt gtgaaaggac ccttttatga
     6961 ccgacgagac acagaccggc gtcgatccgg agccgatcgt ccgcgagatc atgcagacca
     7021 acgtgccgac cgtcgcaccg gaggatacgg tgccggtggt cgccaccatg atggtgcaat
     7081 ccggcatcaa cggcgtgccg gtcattgaga acgggcggat gatcggcatc atcaccgatt
     7141 ccgacatcat cgcgcgcgag gcggacgtgg acgcaccgat gccagtcgtc ttcctcgacg
     7201 cgtatttcac ggccgatgcc gggcgcagct tcgaggagga ggcccgtcgg gcgctggcga
     7261 tcaacgcgcg ccagttaatg agcagcccgg tcgtcagcat tcgggagacg gcgacgctat
     7321 cgcacatcgc gacattgatg atcgacgagg gcgtgaatcc ggtccccgtg ctggacgcca
     7381 gcgacaacct cgtcgggatc gtttcgcgtg cggaccttgt gcgagtgatc gcctgcctgg
     7441 agtccgaggg caccactgat gacacaacgg cgtaatgtcg acgcgctgtt gagcgagtgg
     7501 gatggccggc ccagcatggc gctcgtcgtc ctggaccaac tctcgaccaa cgtgcaggcg
     7561 cttcgccacc gcgtcggttc cgacacgcgc ttcatggcgg tggtcaaggc cgaggggtac
     7621 ggccatgggg cggtgccgat cgccaagaca gccgttcgcg ccggtgctga cgaggtggcc
     7681 gtggcaacgg tcgaggaggg cgtccgcttg cgccggggcg gagtgacggc ccccatcctc
     7741 gtcttgggcc cgatcggcac gaaggaacgt ccccgtgcga tcgctttcga cctcatgcta
     7801 gtgatcggcg accccgattt cgttcgagga ctggccgctg atgtccgaaa gaccggccgc
     7861 aagaccccgc ttgatgtcca cctaaaggtc gacacgggga tgcatcgatt tggcgtgtgt
     7921 cccgaacggg cggtgggcgt ggcgaaggtg atcgacgctg tgccggagtt gcggctggcc
     7981 ggtgtgatga cgcacttcgc atcagcggac gatcccgacg ccgcgtccac gcacatgcag
     8041 gcagccatgt ttgacgaggc ggttgctgcg atccgggcgg ctggcatcga ggtgtcgtcc
     8101 gagcacttgt gcaacagcgc cgcgaccctg aggtacccgg aataccatcg cgaccgcgta
     8161 cgggtcggca tctccatgta cgggctgcgg ccggatcccg agctggtgcc ggttcccgat
     8221 ccgatccggc cgatcatgtc ggtccacagc cgcatcacac gcatcgaaga gctgcagccc
     8281 ggcgagaggg tgagctacgg tggtacctgg caggcggagg cgccgtcgca cgtcgcgctc
     8341 gtgccgatcg gatacgccga tggctattcg cgacagggct cgaatcgagc gtgggtggac
     8401 gtgcgggggc agcggtcgcc ggtgcgtggc cgcgtctgca tggatcagac gatggttgag
     8461 gtcggaccgg atgctcgctc tggtgacatc gtgaccgtaa tcggcgacgg gcagcacggc
     8521 gaggcaccgt cgatcgacga gttagcggtg ctgtggggga cgatctcgca cgaggtggcg
     8581 actggcttcg cggcccggcg gctggggcat ctctacctgc agggcggccg gctggtcgca
     8641 gtcgccgact tgtgggacta ccgcgagatc gacgaggacg ccttcgcgca acctgagatc
     8701 ggtgagcagg atacgacggg cgtggcacag gaggcgcgct gagactcgtt ccgtgatttc
     8761 tcgactgtac catttaccct cgcttggctc gacgttcaca atgccgagtc gcgcactgca
     8821 ctcttcctgc aacgtgtaga attgccgttg cagagcattc ggcgatgcgc gaggtcatgc
     8881 cacacgaagg gggctggcag atatctgcca gcccccttcg tccatccgag ttgttgtggc
     8941 ggctcgccaa tcgtccggct tcgagacccg tgatctagaa ctcgccgccg ccgagctcca
     9001 tctcgatctg cttccgccgt tcgatgtcct tgcggaccac gaaggtgcgg ccgctcatgt
     9061 cgtcgcggac atagttgagc atcttctcat ccatccatcg atagatggtc gggcgggtga
     9121 cacccaactg ctgcgctgcg ttcccgatgc tcaccagctc gctggattcg taggctcgga
     9181 acttggccag cgcgagcatg cggccgaggt ccgtatccca gaacgtgcgc ggaacggtat
     9241 agtcgtctgc tgcgaccggc cagaacagca gctgcaggac cgaatcgatc gcctccagca
     9301 ccacatcctg ggactcgcgg gtctggccgc gtgccaagcg ggccaagcgc agaagatcct
     9361 ggcccaacgg gccgctgcga agttggtcca gcgtcaactg ccggaacgcc tcaggtgaga
     9421 gctgagtcgc cagccggtga tagtggctgt agaccaactg cagcgcgttg tccatgaccc
     9481 tctggtagtc tcgggattct gagtccacga cgactccttt cagatcttgt ctcggccacc
     9541 ccccggcgac cacgaaacgc cgcaacgaca ctgtgtgcag gtgctgtgcg atcaacatgt
     9601 acgcatcaga ctgtacgatc agattgtacg ttgcgagcgg cagagtgtca atcatgaccg
     9661 tacgccgtag ccgagactac cgatgtggtc gtatcggcac agctaacagc catatagcaa
     9721 ccgcagccgg ccgttctccg gtcacgggca actaccctgg atccgggcca gttttcctgc
     9781 ctcactcgcg aaggagggtc gggtctacag gagccgtctg tcggcgcggt tacgcggtgg
     9841 tcatccctcg gatggcgagg ccgaagatga agagcgcgat gaggctgtag atgatca
//
LOCUS       DASYWL010000343         3025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_92906, whole genome shotgun sequence.
ACCESSION   DASYWL010000343 DASYWL010000000
VERSION     DASYWL010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92906"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VGR22_12070"
     CDS             <1..341
                     /locus_tag="VGR22_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007069104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2129344.1"
                     /translation="VRELVGYGRFPHQGLMRSASAEDREAVDWAISVTNLDSFRDREV
                     DTLSGGERQRAWIAMALAQKTDVLLLDEPTTYLDIHYQIEMLSLVRELNQEHGITVGW
                     VLWRSRQVRR"
     gene            639..1193
                     /locus_tag="VGR22_12075"
     CDS             639..1193
                     /locus_tag="VGR22_12075"
                     /inference="COORDINATES: protein motif:HMM:NF018677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrase ferredoxin"
                     /protein_id="HEV2129345.1"
                     /translation="MIGFAPDDAYRVDGMRRIIDMQQPAEVVANSYRRTEYLVPQEGR
                     GLLLRLLAFKPNHPRVAELRTPVDPAARDLLICTRGTVDACCATFGYPIYKLLRAMAD
                     GAPNPTRAWRCTHFGGHRFAATALDLPTGRYWGHLNPETLSKLVHRSAPMRDVRRHYR
                     GWAALSERLWQIAEAELSSETGWM"
     gene            1209..1442
                     /locus_tag="VGR22_12080"
     CDS             1209..1442
                     /locus_tag="VGR22_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129346.1"
                     /translation="MAVSGDVSEPEGGRLTIAYRHPALGGGDVEVEIMPSGSVQTMDV
                     TNAESLREAPQYRARILAQNPTGCLDLMRNAPS"
     gene            1429..1821
                     /locus_tag="VGR22_12085"
     CDS             1429..1821
                     /locus_tag="VGR22_12085"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEV2129347.1"
                     /translation="MPHPDSTLRYWLGQRSAVIVDRPMGSRHPEFPELAYPVNYGYIP
                     NTEAGDMEPLDAYVLGVEVPISSFIGEVIAIVVRHDDTEDKLAVAPTGLRPPRGEIER
                     ATRFQERFFAREIIMHAPDPDTRTPDRP"
     gene            1911..2948
                     /locus_tag="VGR22_12090"
     CDS             1911..2948
                     /locus_tag="VGR22_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HEV2129348.1"
                     /translation="MIDLSSDTATRPTEGMRQAMACAPVGDEQIREDPTVHRLQEYVA
                     NLTGKEAALFLPSGTMCNAIALTVHCRRGDAVILDRRAHPVIAEAGGPAVLADVLLDA
                     IDTPRGRFTSDQVAERAHAGSMHRSRTSLISIENTTNMGGGAIWDVDELVKIRNVATD
                     LGIRVHMDGARLMNAVVGSGRPVTDFTQHVDTVWIDLSKGLGAPVGAVLAGPRTFIED
                     ARRYKHMFGGAMRQAGIIAAAGVYGFEHHIERLAEDHANARALAEGLASLPGISLVHD
                     TIDTNIVWADVADTGLSSAAFYERMMQRGVRVSGWSPSGTLIRAVTHLDVNRADVDRA
                     IEVARDVVAQA"
BASE COUNT          541 a          993 c         1000 g          491 t
ORIGIN      
        1 cggttcgcga gttggtcggc tacgggcgct tccctcatca ggggctgatg cgctcggcat
       61 cggcggagga ccgcgaggcg gtcgactggg ccatctcggt gaccaacctc gacagcttcc
      121 gtgatcgtga ggtcgatacc ctctccggtg gcgagcggca gcgggcgtgg atcgcgatgg
      181 cgcttgccca gaagaccgac gtgctgctgc tcgatgagcc aactacctac ctggatatcc
      241 actaccagat cgagatgctc tcgctcgtgc gggagctcaa ccaggagcac ggcatcaccg
      301 tcggctgggt gctctggcgc tcgcgtcagg tgcgacggtg agcaagcccg atcaactgta
      361 ggggacgact cagaaagatc tccgcgcatg tgaactgctg ggaacgctga tgatcgacac
      421 gacatccggt acggccaact cgcgcgtgac cttctgttcc acggttgctg aggccagcgg
      481 cgaggacccg gccggctcgg cttgggcggt acgacgcatc atcgccgtcg agctgccact
      541 gccgtggccc agccaacgcg ctggaaagcc tcggcgcacc agagggattc actgaaccgc
      601 tctacgaggt attcgaggcg ctggatgagc ggtggggcat gatcggcttc gctccggacg
      661 acgcctacag ggtcgatggc atgcgccgaa tcatcgatat gcagcagcca gccgaggtgg
      721 tcgcgaacag ctaccggcga acggagtacc tggtaccgca ggagggacgt ggcctcctcc
      781 tgcggctgct cgccttcaag ccgaatcatc cccgtgtcgc cgagctgcgc acgccggtcg
      841 atccagccgc ccgtgatctc ctgatctgca cccgcggcac ggtcgacgcc tgctgcgcga
      901 cctttgggta ccccatatac aagctcctac gcgcgatggc cgacggtgca ccgaacccca
      961 cgcgcgcttg gcgctgcacc cacttcggcg ggcaccggtt cgctgccacc gcgctcgacc
     1021 tgcccactgg gcgctactgg ggccatctca acccggagac attgagcaag ctggtccacc
     1081 gttcggcacc gatgcgcgat gtgaggcggc actatcgcgg ctgggcggcc ctgtccgagc
     1141 gcctttggca aatcgccgag gcggagctgt cgagcgagac aggctggatg taggaggata
     1201 ccgaggtcat ggcggtcagc ggtgacgtct ccgaaccgga gggcggccgg ctgaccatcg
     1261 cgtatcggca ccccgcgctc ggaggaggag acgtagaggt cgagatcatg cccagcggct
     1321 cagtgcagac catggacgtc accaacgcag aaagcctgcg cgaggcgcca caatacaggg
     1381 cgcgcatcct cgcccagaac ccgacaggat gcctggacct gatgcgcaat gccccatcct
     1441 gactcgacgc tccgctactg gctgggccag cgatccgcgg tgatcgtcga ccgcccgatg
     1501 ggcagccggc atccggagtt ccccgaactc gcttatccag tcaattatgg gtacatcccg
     1561 aacaccgaag ccggcgacat ggagccgctg gacgcgtacg tgcttggggt tgaggtgccg
     1621 atctcatcgt tcattgggga agtgatcgcc atcgtggtcc ggcacgacga cactgaggac
     1681 aagctggcgg tcgccccgac gggactccgg cctccccgtg gagagatcga acgcgcaacc
     1741 cgcttccagg agcggttctt cgctagagag atcattatgc acgcgcccga cccggatacc
     1801 cggaccccag acaggccgta gcccgcgacg acgtgttggt tctcctgacc gttgcgggta
     1861 cgatagcggc ggcgaatatc gtgatatgcg cagggagcga ccacagactc atgatcgatc
     1921 tatccagcga caccgccacc cgaccgaccg aagggatgcg ccaggcgatg gcttgcgccc
     1981 ccgtcggcga cgagcagatc cgcgaagacc cgaccgtcca ccgtttgcag gaatatgtcg
     2041 ccaacctgac cggcaaggag gcagctcttt tcctgccgtc cggcacgatg tgcaacgcga
     2101 tcgcccttac cgttcattgc cgccgcggtg acgcagtaat tctcgaccgc cgcgcccacc
     2161 cggtgatcgc cgaagccggt ggtccggccg tgctcgccga cgtgctgctc gacgcgatcg
     2221 acaccccgcg cggccgcttc acctccgacc aggtagcaga gcgggcccat gccggcagca
     2281 tgcaccgctc ccgcacgagc ctgatctcca tcgagaacac gaccaacatg ggcggcggcg
     2341 cgatctggga cgtcgatgaa ctggtgaaga tccgcaacgt cgccaccgat ctcggaatac
     2401 gtgtacacat ggatggcgcg cggctgatga acgccgtggt cggcagtggc cggccagtca
     2461 ccgactttac tcagcatgtg gatacggtct ggattgacct gtcgaagggg ctcggcgcgc
     2521 ccgtcggtgc agtgctggcc ggaccgcgga cgttcattga ggatgctcgc cgctacaagc
     2581 acatgttcgg cggtgcaatg cggcaggctg ggatcatcgc ggcggcgggg gtctacggct
     2641 ttgagcacca catcgagcga ctggccgagg accacgccaa cgcgagggcg ctggccgagg
     2701 gtctcgcctc gcttccgggg atctcgctgg tgcacgacac gattgacacg aacatcgtct
     2761 gggccgacgt agctgacact gggctgagct cagccgcgtt ctacgagcgc atgatgcagc
     2821 gcggggtgcg cgtgagcggg tggagtccgt ccgggacgct gatccgggcg gtgacgcacc
     2881 tcgacgtgaa ccgtgcagat gtcgaccggg cgatcgaggt ggctcgcgac gtcgttgccc
     2941 aagcgtaaac cgcccggtgc gggccggcgc aaaccgagcc aacggccttg ctgaacggcg
     3001 cttccttgca tggagcgggc cccgg
//
LOCUS       DASYWL010000344         4388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_337137, whole genome shotgun sequence.
ACCESSION   DASYWL010000344 DASYWL010000000
VERSION     DASYWL010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337137"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1557
                     /gene="recN"
                     /locus_tag="VGR22_12095"
     CDS             <1..1557
                     /gene="recN"
                     /locus_tag="VGR22_12095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEV2129349.1"
                     /translation="DLADAPRDRIIAMLEEQGIEPDKEQLVITREVQASGRSVARING
                     QIVTVSVLNEIGGLLVDVHGQSDHFAIRRKDEQRRLLDRYAGLAPLGEEVRELSGEVN
                     QLRSRLASLSAGERDREQRRDLLAFQVEEIDAADLQPGEDEALANEQRVLRNAEMLRE
                     ETLHAIATLSGDDLGPGGVDVGSTLRQAETGLARIAEVDAAAGGLAERLAEISILTED
                     LGRDLRHYLDTVDVDEERLGEVEERLELIRTLKRKYGSTVEDVLAYRDEAGRELESLT
                     GEEFNTEALEARLIEAEKRLGTVAMKLSQERRHAADRLSAEIEESIASLRMGDGVVQI
                     VTRQVADPRGLSVTIDGDERRVQFDRTGIDDVEILVAPNRGEAPKPLGRIASGGETAR
                     IMLAFKSVLSEHDNTPTLVFDEIDVGVGGRTGQVVGERLRDLAERHQVIVITHLPQIA
                     AMAHKHARITKRQVEDRAISVVTELQDGEVEHEIAAMLDGEPVTAASIESAREMIARS
                     HRYQPRAQAS"
     gene            1651..1941
                     /locus_tag="VGR22_12100"
     CDS             1651..1941
                     /locus_tag="VGR22_12100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEV2129350.1"
                     /translation="MKMIQQMQNRMVKIQEELEEAEVEGSAGGGVVTATATGQRVLKG
                     IKIDPSAVDPDDVEMLEDLVLTAVQDAMEKAAALAEEKMSALTGGMKLPGLM"
     gene            1988..2194
                     /locus_tag="VGR22_12105"
     CDS             1988..2194
                     /locus_tag="VGR22_12105"
                     /inference="COORDINATES: protein motif:HMM:NF017879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEV2129351.1"
                     /translation="MGDIVRLRKPHPCGSHEWEVVRLGADIGLRCQGCKRRVLLPRRT
                     LEQRLKRFISRGPDAPEPDESQGR"
     gene            2191..3570
                     /locus_tag="VGR22_12110"
     CDS             2191..3570
                     /locus_tag="VGR22_12110"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2129352.1"
                     /translation="MSAQATSRQHEDTIRAVLSNGAFLRLWIVQAVTQVAQNMINFAL
                     LLRVRSVVETHDITQANTAISLVILAFSLPAVLFGPFAGVVADRVNKRLLMAVLNIAR
                     AVAVLLFLFIRPEWHPETILIAIYVGTFLFGIAGQFFAPVLGATIPIVTPRNQLVSAN
                     ALFNLTFTGAQLAGFAAAGPVFAKLLGIDTLFAVTVVIYIACAALVMTIPPTPVHAVK
                     VPGGFNLSVVMSDIKEGLVYILQDPILIRAIGYLTLAQTTFLMIAALGPEFITGIVGL
                     DKEDIGYIVAPAGVGVLVGVLLVPRVARRFGRGWLIDIALTLAGVMLGGIAAVRYVLD
                     GLWLDGLAPVWLVTIAAALLATALGLCNAFILVPSQTMLQERSHEAVRARVYATFFTV
                     SNTASFVPIFFAAAFADLFGVVQVFAVLAAAVIGLGASALIHRRRAETNRWERERTLH
                     RQGPEVLRR"
     gene            complement(3608..>4388)
                     /gene="pyrB"
                     /locus_tag="VGR22_12115"
     CDS             complement(3608..>4388)
                     /gene="pyrB"
                     /locus_tag="VGR22_12115"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEV2129353.1"
                     /translation="FYEPSTRTRLSFESAMLRLGGQVISTENAREFSSAIKGETVEDT
                     VRIIAGYADGVVIRHYERGAAARAAAVSPVPVINGGDGPGEHPSQALLDLYTIHHELG
                     TFDGLTIALVGDLRYGRAARSLALLLGMSRGTRLIFVAPPAVPMGMDVKQALDRRGVV
                     WQDTPDLAAAMSESDVVYQTRIQRERFATPDEARAAEGHYLINAATMTHMKQRSILLH
                     PLPRVEEISADVDSDPRAAYFRQAENGVYVRMALLHALLST"
BASE COUNT          761 a         1372 c         1493 g          762 t
ORIGIN      
        1 gacctcgccg acgccccacg ggaccggatc atcgcgatgc ttgaggaaca gggcatcgag
       61 ccagacaaag aacaactggt tattactcgc gaggtgcagg cgagcgggcg ctcggtcgcg
      121 cggatcaacg ggcagatcgt cacggtgagc gtgctcaacg agatcggggg gttgcttgtc
      181 gatgtccacg ggcagtcgga tcacttcgct atccggcgca aggatgagca gcgacgcctg
      241 ctggatcggt atgcgggact cgcgccgttg ggcgaggagg tccgggaatt gagcggcgag
      301 gtcaaccagc tccgatcgcg ccttgcgtcc ctctccgcgg gtgagcggga tcgggagcag
      361 cggcgcgacc ttctggcgtt ccaggtcgag gaaatcgatg cggcggacct ccaaccgggc
      421 gaggacgagg cactcgcgaa cgagcagcgt gtgctcagaa acgccgagat gctccgcgag
      481 gagacactac acgccatcgc cacgctttcg ggtgacgatc tcggccccgg gggtgtggac
      541 gtgggctcga cgctgcgcca ggcggagacc gggctggctc ggatagccga ggtggatgcc
      601 gctgccggcg gcctcgccga gcgtctcgct gagatttcga tcctgactga agacctcggg
      661 cgcgatcttc ggcactatct cgataccgtc gacgtcgacg aggagcggct gggagaggtc
      721 gaggagcggc tggagttgat ccgtacgctg aagcgcaagt acggcagcac ggtcgaggac
      781 gtgctcgcgt accgtgacga ggccggccgt gagttggagt ctctcacggg cgaggagttc
      841 aatacggaag ctctggaagc tcgcctcatc gaggcggaga agcgattggg cacggtcgct
      901 atgaagctct ctcaggagcg ccggcacgct gccgaccgac tctccgccga aatcgaggag
      961 agcattgcgt cgctgcgcat gggcgacggc gtcgtgcaga ttgtcacccg ccaagttgcc
     1021 gacccgcggg ggttgtcggt cacaatcgac ggcgacgagc gtcgggtcca gttcgaccga
     1081 accgggatcg acgacgtcga gattctcgtc gcgccgaacc gcggcgaagc gccgaagccg
     1141 cttggtcgca ttgcctctgg tggcgagacc gcccgcataa tgctggcgtt caagtccgtg
     1201 ctctctgagc acgacaacac accgaccctc gtgttcgacg aaatcgacgt cggcgttggt
     1261 ggccgaaccg gacaggtcgt cggggagcgg ctgcgagacc tggccgagcg gcatcaggtg
     1321 attgtgatca cgcacctgcc gcagatcgcc gccatggccc acaagcacgc ccggatcaca
     1381 aagcgacagg ttgaggaccg agccatctcg gtggtgacgg agttgcagga cggcgaggtt
     1441 gagcacgaga ttgcggcgat gctggatggc gagccggtga cggccgcctc gatcgaaagc
     1501 gcccgggaga tgatcgctcg ctcacatcgc taccagcctc gggcccaggc ttcgtagacg
     1561 ccctccgcta tactgatggc acgaaaccgg ccgattgcgt cagagcgatg tgccaagaca
     1621 acaaccaggg gaatgaccgt gcagccaaac atgaaaatga tccagcagat gcagaaccgg
     1681 atggtcaaaa tccaggagga actggaagag gccgaggtcg aggggagcgc gggtggcggc
     1741 gtcgtgacgg cgacggccac tggccagcgc gtactcaagg gcatcaagat cgacccatcg
     1801 gccgtcgatc ccgacgacgt cgagatgctc gaggatctcg ttctcaccgc agtgcaggac
     1861 gcgatggaga aggccgctgc gctcgccgag gagaagatgt cggcgctcac cgggggcatg
     1921 aagctccccg gcctgatgta gcgctcgacc ggtgtccgtg gtgcttcgcg agccgatccc
     1981 gttctacatg ggcgacatcg tgcgcctgcg aaaaccgcac ccatgtgggt cgcacgagtg
     2041 ggaggtggtt cggctcgggg ccgacatcgg gctgcggtgc cagggatgca agcgccgggt
     2101 tctgctgccg cgacggacgc tggagcagcg cctgaagaga tttatctctc ggggaccgga
     2161 cgcgcccgag cctgatgagt cgcaaggccg atgagcgcgc aggccacgtc acgtcaacat
     2221 gaggatacga tccgcgccgt tctctcgaac ggcgcgtttc tgcgcttgtg gatcgtccag
     2281 gcggtcacgc aggtcgcgca gaacatgatc aatttcgcgt tgctgctgcg cgtccgaagc
     2341 gtcgtcgaga cgcacgacat cacccaggcc aacacggcga tcagcctcgt catcctcgcg
     2401 ttctcgttgc ccgccgtcct gttcggaccg ttcgccgggg tggtggccga ccgtgtcaac
     2461 aaacgcctgc tgatggccgt gctgaacatc gctcgggcag tcgcggtgct gctctttctc
     2521 ttcatccggc cagagtggca tccggaaacg attctcattg ccatctacgt cggcaccttc
     2581 ctgttcggga tcgccgggca attctttgcg ccggtcctcg gtgcgacgat cccgatcgtc
     2641 acgccacgga accagcttgt ctccgcgaac gcgctcttca acctcacgtt cacgggcgcc
     2701 cagctggccg ggttcgcggc cgccggcccc gtgttcgcca agctcctcgg catcgacacg
     2761 ctgtttgcgg tgacggtggt catctacatc gcctgcgcag cactcgtcat gacgatcccg
     2821 cccacgcccg tgcatgctgt caaggtgccc ggcggtttca acttgtcggt ggtgatgagc
     2881 gacatcaagg agggcctcgt ctacatcctg caggacccga tcctgattcg agcgatcggc
     2941 tacctgacgc tcgcccagac gacgttcctg atgatcgcgg ctttgggacc ggagttcatc
     3001 actggcatcg tcggcctcga caaggaggac atcggctaca tcgtcgcgcc ggccggagtc
     3061 ggcgtactgg tcggcgtgct gctcgtgcct cgcgtggcgc gacgatttgg ccgcggctgg
     3121 ctgatcgaca tcgcgctcac gctcgcggga gtcatgcttg gcggcattgc ggccgtacgc
     3181 tacgtgctgg acgggttgtg gctcgacggg ttggcgccgg tctggctggt cacgatcgcc
     3241 gcagcgttgc tggcgaccgc cctcggtctc tgcaacgcct tcatcctcgt gccctcccag
     3301 accatgttgc aggagagatc gcacgaggcg gtccgggcac gggtgtacgc aaccttcttc
     3361 accgtctcca acacggcatc cttcgtgccg atcttcttcg cggcggcgtt cgccgatctg
     3421 ttcggcgtcg tccaggtatt cgcggtgctg gccgccgcgg tgatcggact gggggcctct
     3481 gctctgatcc accgtcgccg ggcagagacg aaccgatggg aacgcgagcg gaccctccat
     3541 cggcagggcc cggaggtcct tcgacgttga gtgaccccgc ccggttgccg ttgcgcgggc
     3601 cctgtgatta tgtcgacagt agtgcgtgca gaagcgccat gcggacgtag acgccattct
     3661 cggcttggcg gaagtaggca gcacgcgggt cgctgtcgac gtccgcggag atctcctcaa
     3721 cgcgtggaag agggtgcagc aggatcgacc gctgcttcat gtgcgtcatt gttgctgcgt
     3781 tgatgaggta gtgtccctcg gctgcacgcg cctcgtcggg cgtggcgaag cgttcgcgct
     3841 ggatgcgcgt ctgataaacc acgtccgact ccgacatggc cgcggcgaga tccggagtat
     3901 cttgccagac cacgccccgc cgatcgaggg cctgctttac gtccatgccc atgggaacag
     3961 cgggcggggc aacgaagatc agccgcgtac cgcgcgacat gccgagcaac agggcaagcg
     4021 acctcgccgc gcggccgtaa cgcagatcgc caaccagggc gatggtgaga ccgtcgaacg
     4081 taccgagctc gtgatggatc gtgtagagat cgaggagcgc ctggctggga tgctcgccgg
     4141 gaccgtcgcc gccgttgatc accggcaccg gagatacggc cgccgctcgg gcggcggcgc
     4201 cacgctcgta gtgccggatc acgaccccgt cggcgtagcc ggcaatgatc cgcaccgtgt
     4261 cctcgacggt ctcgcccttg atcgccgagg agaactcgcg cgcgttctcg gtggagatca
     4321 cctgcccacc aagacgcagc atcgcactct cgaagctgag gcgggtacgg gtcgagggct
     4381 cgtagaag
//
LOCUS       DASYWL010000345         4067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_130501, whole genome shotgun sequence.
ACCESSION   DASYWL010000345 DASYWL010000000
VERSION     DASYWL010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130501"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /locus_tag="VGR22_12120"
     CDS             <1..1014
                     /locus_tag="VGR22_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2129354.1"
                     /translation="PAVNGIDLRTEDGEFVVLLGPSGCGKTTLLRMIGGLEKPTSGEI
                     LIGDRVVNDLPPRARQIAMVFQSYALYPHMTVYNNISFPLKAAKLGKQEIDQKVRWAA
                     GILGIDQYFDRKPRQLSGGQRQRVALARALVREPNVFLLDEPLSNLDAQRRASARDEL
                     QVFQREVGTTTIYVTHDQVEAMGLGDRIAVISEGSIRQFGTPHEIYEDPADTFVATFL
                     GSPPMNLVERGDELLGFRPEAVEFAKSTENLDGSSWLTLTLAETREEYLGSEKLIYGE
                     IGGERVVARTAASMPMPVEEGGDIEIAIPNSALRIFDKGTGLRKRESTLRISTSSTEE
                     VRA"
     gene            1011..1958
                     /locus_tag="VGR22_12125"
     CDS             1011..1958
                     /locus_tag="VGR22_12125"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV2129355.1"
                     /translation="MSSAPTTGERYTFEREGILGPLFLLPAVVYILALVGLPLVLAVL
                     YAFSGVTVGDPRITRDDFVGLDNFRAILNDSTFRKALRNTLVFTGISQLIVIILANLL
                     AMVLTANFRGKRFVRFLVLLPWTTPVALGVLGWFWMLDSVYSPIDWLLREIGLLGPDT
                     PFGPARNMYWLGTSHLAQGAVILVHIWRTLPLATVILLAGLTAIPQDIKDAADIDGAG
                     FWRQLVYIRLPLLLPIMAVALLFAVVFAFTDLTIALVLTRGGPVNDTQVLASWAYFKG
                     IQGGDLAQGAAIALFLLPVLVAIAIAMLRLARRGGGEGA"
     gene            1962..2873
                     /locus_tag="VGR22_12130"
     CDS             1962..2873
                     /locus_tag="VGR22_12130"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV2129356.1"
                     /translation="MSRQTAVTGRAPVSDLHRERAETYRGKRIAGRIVLYFAATFFAM
                     FAALPFAWMVLTMFKADSDLYNPNNNPFVYNDPPTMANLDLLWNQTSYRTFIFNTLLV
                     ALFVVLITVVIVVPAAYALTRLTGRWGESLGILIFLVYLVPPTLLFIPLTGVVADVGL
                     QNSKWSMVLVYPTFTIPFCTWLLMGFFRGVPWDIEEQAMIDGYSRIQAIWKTVIPVSL
                     PGILTVVIFSFTLTLHEFVYALAFVQSSGEKTVSVGVFTELIRGDVYFWQSLMAAAAL
                     VAFPVALLYNYFLDRFIAGFTLGAVKG"
     gene            3109..>4067
                     /locus_tag="VGR22_12135"
     CDS             3109..>4067
                     /locus_tag="VGR22_12135"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2129357.1"
                     /translation="MPFALQLPRTLPYYVALALLFASSGTMWGIWIARIPSVANKLDM
                     GTAQLGAVLPAFSAGALVAFPVTAGLTSRCGSRTIIRAAGVMRGLLFPALALAPNTFW
                     LMVALALAGFTHGALDIALNAQGVEIERRTVGSILSRAHGSFSLGALVGSVSIGFAAT
                     AGLPLEWQFIIPGVPAVLMFSTVSTLLIPDDTTPTPTTQRLAAKRKWRGAGMLPRALW
                     TIGAIALITGIADEAIADWAALLIQRELEATPLVASLAYSVYSLALLVGRFSGDGISE
                     RLPSMRILQIAGVMGAVGMIGSAALNSPYAMIGGIAVIGLGFSV"
BASE COUNT          627 a         1419 c         1236 g          785 t
ORIGIN      
        1 cccgcggtca acggcatcga tctccgaacc gaggatggcg aattcgtggt gctgctcggc
       61 ccgtccgggt gtggcaagac cacgctcctc cgcatgatcg gtggcctgga aaagccgaca
      121 tctggcgaga tcctcatcgg ggatcgcgtc gtgaacgatc tgccgcctcg tgcgcggcag
      181 atcgcgatgg tcttccagag ctacgcgctc tacccgcaca tgaccgtcta caacaacatc
      241 tcgttcccgc tcaaggcggc caagctcggc aagcaggaga tcgatcagaa ggtgcggtgg
      301 gctgcgggca ttctcggcat cgaccagtat ttcgaccgca agccgcgcca gctttccggc
      361 ggccaacgac aacgcgtcgc gctggcccgg gcgctggtcc gcgagccgaa tgtcttcctg
      421 ctggacgagc cgctctcgaa cctggacgcg cagcgccggg cctcagcccg agacgagctg
      481 caggttttcc agcgcgaggt cggcaccacc accatctatg tcacgcacga tcaggtcgag
      541 gcaatgggcc tcggcgaccg gatcgcggtg atcagcgagg gcagcattcg gcagttcggc
      601 actccgcacg agatctacga agacccggcc gacacctttg tcgcaacctt cctcggctcc
      661 ccgccgatga acctcgtcga gcgtggtgac gagctgctcg ggttccgccc ggaagcggtc
      721 gagttcgcca agtccactga gaacctggac ggcagctcct ggctgacact gacgctcgcg
      781 gagacacgcg aggagtacct cggctcagaa aaactcatct atggtgagat aggcggcgaa
      841 cgcgtggtgg ctcgcacggc ggcgtcgatg ccgatgccgg ttgaggaggg tggcgacatc
      901 gagatcgcca tcccgaacag tgcgcttcgc atcttcgaca agggcaccgg cctgcggaag
      961 cgtgaatcga cgctgaggat ctcgacctcc agcactgagg aggtgcgcgc atgagctccg
     1021 cgccaaccac cggcgagcgc tataccttcg agcgcgaggg gatcctcgga ccgctcttcc
     1081 tgctgccggc ggtggtctac atcctcgcct tggtcggcct gccgctcgta ctcgccgtgc
     1141 tctatgcctt cagcggcgtc acggtcggag acccgcgcat cacccgcgac gatttcgtcg
     1201 ggctggacaa cttccgcgcg atcctcaacg acagcacctt ccggaaggcg ctgcgcaaca
     1261 cgctcgtctt caccggcatc tcccagctga tcgtgatcat cctcgccaac ctcctggcga
     1321 tggtgcttac cgccaacttc aggggcaagc ggttcgtccg cttcctcgtg ctgctgccgt
     1381 ggacgacgcc cgtggcgctg ggcgtgctcg gctggttctg gatgctcgac tccgtctaca
     1441 gcccaatcga ctggctgctg cgcgagattg gcctcctcgg gccggacacg ccgttcggac
     1501 cggcccggaa catgtactgg ctcggcacct cccacttggc gcaaggtgca gtaatcctcg
     1561 tccacatctg gcgcacgttg ccactggcga ccgtaatcct gctcgccggc ctgactgcga
     1621 tcccgcagga catcaaggat gccgcggata tcgatggcgc cggcttctgg cgacagctcg
     1681 tctacatccg cctgccgctg ctgctgccga tcatggccgt cgccctgttg ttcgcggtgg
     1741 tcttcgcctt caccgacctc acaatcgcgc tcgtgctgac gcgcggcggt cccgtcaacg
     1801 acacccaggt cctcgcgtca tgggcctact tcaagggcat tcagggcggt gaccttgctc
     1861 agggcgccgc aatcgcactc ttcctgctgc ctgtgctggt cgccatcgcc atcgccatgc
     1921 tccgcctcgc gcggcgcggt ggtggggaag gagcctgatc catgtcccgc cagaccgccg
     1981 tgaccggccg cgcacccgtc tccgaccttc atcgcgaacg ggccgagacg taccgcggca
     2041 agcgcatcgc cggccgcatc gtgctctact ttgcggcgac cttcttcgcg atgttcgcgg
     2101 ccctgccgtt cgcgtggatg gtcctgacga tgttcaaggc cgacagcgac ctctacaacc
     2161 cgaacaacaa cccgttcgtc tacaacgacc cgccgacgat ggccaacctg gatctcctct
     2221 ggaaccaaac cagctaccgc accttcatct tcaataccct gctggtggcg ctcttcgtgg
     2281 tgctcatcac ggtggtgatc gtggtgccgg cggcctatgc gctcacccgg ctcaccggac
     2341 gctggggcga gagcctcggc atcctgatct tcctcgtcta cctggtgccg ccgacgctcc
     2401 tcttcatccc gctgacgggc gtggtggccg acgtcgggct gcagaactcg aagtggtcga
     2461 tggtgctcgt ctacccgacg ttcaccattc ccttctgcac gtggctactc atggggttct
     2521 tccgcggcgt gccatgggac atcgaagagc aggcgatgat cgacggctac agccgcatcc
     2581 aggcgatctg gaagaccgtc attcccgtgt cgctgccggg catcctcacg gtcgtcatct
     2641 tctcgttcac gctgaccctc cacgagttcg tctacgcgct ggcgttcgtg cagtcctcgg
     2701 gggagaagac cgtgagcgtt ggtgtcttca ccgagttgat ccgcggtgac gtctacttct
     2761 ggcagtcgct gatggcggcg gcagcgctgg tcgcctttcc ggtggcactg ctctacaact
     2821 acttcctcga ccggttcatc gccggcttca cgctcggcgc cgtcaaggga tagcacaacg
     2881 acgcacgctg acctttgtgc ggtcgggccg accctgcaaa ggtgcgtggt acgcccagca
     2941 tgccccgtgc acgaccaacc gatctgcagg acaccggaca ggcatgcacg gttgccctgt
     3001 cgattgccgg gcgatatagt tctgccggac agcggtcacc ccgctgccga tcccttttcg
     3061 ggttctgcca agtcactgcc ttcctgccca gccgcgagat ggtcccgtat gccgttcgcc
     3121 ctccaactgc cgcgaacgct gccctactac gttgccctcg ctttgctctt cgcatcgagc
     3181 ggtacgatgt ggggcatctg gatcgcgcgt atccccagcg tcgcaaacaa gctcgacatg
     3241 ggcacggccc agcttggcgc ggtcctgccc gccttctcgg ccggggccct ggttgccttc
     3301 cccgtgacgg cgggcctgac aagtcgttgc ggcagtcgta cgatcattcg cgccgccggt
     3361 gtgatgcgtg gattgctctt tccagcactc gccctcgccc caaatacgtt ctggctgatg
     3421 gtcgcgctgg ccctggcggg attcacccac ggcgcgctcg acatcgccct caacgcacag
     3481 ggcgtcgaga tcgagcgccg caccgtcggc tcgatcctct cccgcgcgca tgggtcgttc
     3541 agcctcggcg ccctggtcgg ctcggtcagc atcggtttcg ctgcgaccgc agggctgccg
     3601 ctcgagtggc agttcatcat ccccggcgtg cccgccgtgc tgatgttctc gaccgtcagc
     3661 accttgctca tccccgatga cacgactcca accccaacaa cgcagcgtct ggccgcgaag
     3721 cgcaagtggc gaggcgctgg catgctgccg cgtgcgctgt ggacgattgg cgcgatcgcg
     3781 ctgatcaccg gcatcgccga cgaggcgatc gccgactggg cggccctgct catccagcga
     3841 gagctggagg ctaccccact ggtcgcctcg ctcgcctact ccgtctactc gctggcgctg
     3901 ctagtcggcc ggttctccgg cgacggcatc agcgaacgtc tcccgtcgat gcgtattctg
     3961 cagatcgccg gggtcatggg cgccgtcggg atgatcggga gtgcagcgct caactccccg
     4021 tacgcgatga ttggcggcat tgccgtcatc ggtctcggct tcagcgt
//
LOCUS       DASYWL010000346         7774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_637050, whole genome shotgun sequence.
ACCESSION   DASYWL010000346 DASYWL010000000
VERSION     DASYWL010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7774
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637050"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VGR22_12140"
     CDS             <1..525
                     /locus_tag="VGR22_12140"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV2129358.1"
                     /translation="APCYGEAERRSPMSMFCQRCGAPTHDVCRDGRMRPVCTSCGTTT
                     YLDPKLAVAVLIGREEEVLLGRRGVHTREPGKWSFPSGFVERGERVEDAARRETLEET
                     GLTVELGPVLTLISAPGETVVLVVYPAIAFSGEPSADDDLAELRWWRLDALPELAFPH
                     DLAIIRMWLGSRKH"
     gene            complement(842..1120)
                     /locus_tag="VGR22_12145"
     CDS             complement(842..1120)
                     /locus_tag="VGR22_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEV2129359.1"
                     /translation="MANASKDDLIKGLNDDLAGEYNAIISYLQYSAKVNGPYRPQLVE
                     FLQADIPDEQAHAQYLADKIASLGGEPTVETTTGEDIRRHARDAPVHL"
     gene            1402..2031
                     /locus_tag="VGR22_12150"
     CDS             1402..2031
                     /locus_tag="VGR22_12150"
                     /inference="COORDINATES: protein motif:HMM:NF013209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEV2129360.1"
                     /translation="MGKKTDKAIAKLRSDVQALSEAVWALKEHVRVEAAAESAAAGSR
                     KRTSRKLRQIETQLADGEAQGRVSGYGSVRLPGANGVSRVVRWQLENVTTDSVLPIDL
                     EPAAAVLSAVGHRQRLSILIALLRQPMSVSELVSSLDLGTSGAAYHHLNVLQNVDLVA
                     QQERGIFELAPQQVATVIGMLSALAVSPTVETEESVPGQEPDGGADSAE"
     gene            2345..2752
                     /locus_tag="VGR22_12155"
     CDS             2345..2752
                     /locus_tag="VGR22_12155"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV2129361.1"
                     /translation="MRTFPKPLIEAERDWEVDAIHPRAVMEARRTLRNAAACSDVASL
                     FAVLGDPTRIRVLTALAAGELCVCDLAAATGVNRSTVSHQLRVLRTHRLVRRRREGKV
                     AYYALDDDHVLQLLAVAGEHSSETGAGRKEVTA"
     gene            2749..4992
                     /locus_tag="VGR22_12160"
     CDS             2749..4992
                     /locus_tag="VGR22_12160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEV2129362.1"
                     /translation="MSDRTDAEPTAEADAPYVFDVTGMDCADCARSVERAVWHLPHVE
                     RATVNFASGTLTVGRRDPRGSDDDLVRSVNAAVDRAGYTAALRGESGRRVTATTPWWR
                     HRKLIPATVGAALWFAGFTVGQLTELDWLEVALFAAAIVLGGHQIIRAGVAALQARRI
                     DMNVLMTVSVIGAAILREWSEGALVVVLFSVGTTLQAITFDRTRNAIRSLLDLTPQEA
                     RVVRDGVETTVAANSLEPGDLVRVRPGERLPADGEVVEGFSAIDQAPITGESMAVERE
                     AGDEVFAGTLNGSGVLDIRVTKRSSQSMLASIVHLVEEAQASRAPSQQLVDRFAAVYT
                     PIVVALAAVIALAGGALAGDWDTWVYRALVLLVIACPCALVISTPVSIVSAVGAASRA
                     GILVKGGAALEGAGRAKVVAFDKTGTLTLGRPAVTAVVPYGDHGEQDLVRIAAAVEQG
                     SEHPLARAVVARALYDDVPIPAAPGFSAIPGRGARASVDGAEIVVGSERLMLESGLQG
                     GARRWLADVTLAEAAQGRSVLAVGTSSGDLYGAIVIADKIRPRAREALGALRRVGVER
                     LVVLTGDRQEVAERIAAEVGVDEVRAELLPDEKAAVVADLRKRYGPVAMVGDGINDAP
                     ALAAADVGIAMGVAGSDVALESADLALMRDDLEATSRLFGLSRRTVAIIRQNVTLSLI
                     TKLLALLLGVFGFVSLWIAVLADVGTSVVVTLNGLRLARVEQRVGQPVPREERRAEDA
                     DRPLAAD"
     gene            complement(5299..6159)
                     /locus_tag="VGR22_12165"
     CDS             complement(5299..6159)
                     /locus_tag="VGR22_12165"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2129363.1"
                     /translation="MSEHISIDDNAVANIASEDPATTIADDIAYHHLGIVNAVYIGMP
                     GSVDWVLVDAGIPGTARFIRKAVEERFGKGAKPAAIIMTHGHFDHVGALKSLASDWEV
                     PIYAHPLEVPYLSGSTAYPAPDPSVGGGMMAALSRFFPKGPVDVKEWLQTLPPDGSVP
                     QLPGWRWLHTPGHTEGHISLWRESDRSMIAGDAFVTTDQESIYAVAKQKPALQGPPTY
                     LTPDWPSARQSVRTLADLEPELAIAGHGPALHGPELKIKLKLLADRFDEIAVPDHGKY
                     VPEGMRDEDT"
     gene            complement(6329..6856)
                     /locus_tag="VGR22_12170"
     CDS             complement(6329..6856)
                     /locus_tag="VGR22_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129364.1"
                     /translation="MRSTHDRRSSLRTRRPDDTPLLPQQAESSSLKPAGTNSLDIRLS
                     GGGETVAETRTYELPESDEAATFAERAEIYTGATNVQSGGTETVDGVETKILTFYLLS
                     KTGQSGAWFAWWVDPDTGNLLRLAMIAKMHFMVWNLTDINERFTIEPSPVSAVATPLP
                     SDPQGPAGVSGQLSR"
     gene            complement(6828..>7774)
                     /locus_tag="VGR22_12175"
     CDS             complement(6828..>7774)
                     /locus_tag="VGR22_12175"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2129365.1"
                     /translation="DLCRDRPEGARPMSSQAMTRTETATLEEMGRMPPARSERSRIWR
                     RFVRYKPGLVGLTVIGLLVILAVFAPWIAPHSPTETETSLRGAAPSAEYLLGNDDIGR
                     DILSRIIYGARVALIVGLGATAIAVTIGVTIGATAGYFGGRVDFILSRFVDTLMAFPL
                     LALLLTLSSVFGPSLRNVVIVIGLTVWASYARLVRAEVLSLRERDFVLAARAIGAGDR
                     RIIFRHIVPNALGPVIVIASLAIGGVIILESALSFLGLGVSRPTASWGTMLSDGREFI
                     RNYPHIAIAPGIAITITVLSFNLVGDGLRDALDPRQKE"
BASE COUNT         1249 a         2396 c         2728 g         1401 t
ORIGIN      
        1 gctccatgct acggtgaggc cgaacggagg agcccgatgt cgatgttctg ccagcgctgt
       61 ggcgcgccaa ctcacgatgt ctgccgggat ggtcgcatgc gaccggtctg cacgtcatgc
      121 ggaacgacga cctaccttga cccgaagctc gctgtcgcgg tgctcatcgg gcgggaagag
      181 gaagtgctgc tgggccgccg tggcgtacac acccgggaac cgggcaagtg gagttttccc
      241 tctggttttg tcgagcgcgg ggagcgcgtc gaggatgccg cccgccgcga gacactggag
      301 gagaccgggc tcacggtcga actcggtccc gtgctgacac tgatctccgc tccgggcgag
      361 acggtcgtgc tcgtggtcta ccccgccatt gcgttcagcg gtgagccgag cgcagacgac
      421 gatcttgccg agctccgctg gtggcggctc gacgccctgc cggagctggc cttcccacac
      481 gatctcgcga tcatccgcat gtggcttggc tcccgcaagc actgagtcgg gcaggctcgc
      541 agcgtttcgg cggcatacgc cctgccactg gtccgattcc gagttgccct gtagcaagaa
      601 tgccggaatg gagaacgccc ggagcttgaa ttgccccggg cgttggtgat gccgctccga
      661 tttgtgatgg agcaaggccg cacgctcgtt ccatgatccg ccgaggatct tcttgacctc
      721 gtcgcggtga cgtgtctcga cgctgagcat gtcctccagc tgtaccttta ggccggtttc
      781 tccgcaggcc tccgcctggt cgatgcgctt gcggtagttc tcgacggtgt ctcgttccgc
      841 ctcatagatg aactggagca tctcgcgcgt gtcgccggat gtcttcaccg gtcgtggttt
      901 cgaccgttgg ctcgccaccg agtgacgcga tcttgtcggc caggtattgt gcgtgcgcct
      961 gctcgtccgg aatatccgcc tgcaggaact cgacgagctg tggtcggtag ggaccgttga
     1021 ccttcgccga gtattggagg tagctgatga tcgcgttgta ctcgccagcg agatcgtcgt
     1081 tcagaccctt gatcagatcg tccttgcttg cattggccat cgtgaataac ctcctgccga
     1141 taggggagca tttgccgtcg ctcggactcc tcaacttccc ttcgagcaaa ttccgtacca
     1201 gccaggaacc ctcgggaggt gccgctacgc aattcattcg gccgttcatg ccgccggtgt
     1261 actccaagtc ggccgcgagc ttggtagaga gtgggccgta tcgcgcaggc tgcggcgtgc
     1321 tggagcaggt gcggtatgat gacggaaacc cccaaatcga ataggaaccc gcatcgggtg
     1381 cgtcgcgtgg agggctggac gatgggcaag aaaaccgaca aggcgatcgc caagctccgc
     1441 tcggatgtcc aggcgctctc ggaggccgtt tgggcgctga aggagcacgt ccgagtcgag
     1501 gcggccgccg agtcggctgc cgccggatcg cggaagcgca cgagccgcaa gctgcgtcaa
     1561 atcgagacgc agttggccga tggcgaggct cagggtcgcg tgagtggcta cggtagcgtc
     1621 cggctgccgg gagccaacgg tgtgtcgcga gtagtgcgct ggcagctcga gaacgtcacg
     1681 accgacagcg ttctgccaat tgatctggag cccgcggcgg cggtgctctc cgccgtcggg
     1741 catcgtcagc gattgtcgat cctgatcgcg ctgctgcggc agccgatgtc ggtaagcgaa
     1801 ctggtgagct cgcttgacct cgggacctcc ggagcggcct accaccacct caacgtgctc
     1861 cagaacgtgg atctcgtcgc ccagcaggag cggggcatat tcgagctggc accgcagcag
     1921 gtggcgaccg ttattggcat gttgtcggcg ctggcggttt caccgacggt ggagaccgag
     1981 gagtccgtcc ctgggcagga gccggacggc ggggcggatt ccgccgagtg acgggccgtc
     2041 gcaaagggac gactccgcaa tgctccgtac ggcggcatgc gtcggactga cccggggcga
     2101 gctgtgaccg gggcagtcgt gatgtcgtca gggctgaccg ccgttatggc tgacagcatc
     2161 gaggagggca attgtggaga cgaggatcgg cagctctctg cgggggtcgg aatgactttc
     2221 cagcgagccg actcacctcg gtcgggtttc cgcgcacaaa cgtgtgtttg caggtacaca
     2281 tgtgcattgg tagactcgcc atagacagtc atgtgtatga cgatgcacat atcaggagga
     2341 gcccatgcgg acgttcccca agcccttgat cgaggcagag cgcgactggg aggttgacgc
     2401 gattcatccc cgcgcggtga tggaggcacg caggactctc cgcaatgctg ctgcatgcag
     2461 tgacgttgcc agtctcttcg cggttctcgg cgatcccacc cggatccggg tgctcacggc
     2521 gctcgcggca ggggagctct gtgtctgcga tcttgccgca gcgacgggtg tgaaccgcag
     2581 cacggtctcc catcagctgc gcgtgctccg aacccaccgg ctggtccggc gtcgccggga
     2641 ggggaaggtc gcctactatg cactcgacga tgaccacgtg ctgcagctgc tcgctgtggc
     2701 aggtgaacac agcagcgaga cgggggccgg tcgaaaggag gtgaccgcat gagcgaccga
     2761 acggacgccg agcccacggc cgaggccgat gcgccctatg tcttcgatgt gaccggcatg
     2821 gactgtgctg actgtgcccg cagtgttgag cgcgctgttt ggcaccttcc ccatgtggaa
     2881 cgcgctacgg tcaatttcgc ctcaggcacc ctcaccgtgg gacgacgcga tcccagaggc
     2941 agtgacgatg atctcgttcg gtcagtgaac gcagccgtgg accgtgctgg ctacaccgcc
     3001 gcgctgcgtg gtgagagcgg gcggcgggtg acggcaacca cgccctggtg gcggcaccgc
     3061 aagctcatcc ccgccaccgt cggtgccgcg ctctggtttg cgggcttcac cgtcggccag
     3121 ctgactgagc tcgactggct cgaggttgcc ctcttcgccg ccgcgatcgt gctcggtggg
     3181 caccagatca tacgcgccgg tgtggcagcg ctgcaggctc gccgcatcga catgaatgtg
     3241 ctgatgaccg tctcggtgat cggcgcggcg atcctccgcg agtggtccga gggcgccctg
     3301 gtagtggtgc tcttttcggt cggcacgacc ctccaagcga tcacgttcga ccgcacccgc
     3361 aacgcgatcc gctcgctgct cgacctgacg ccgcaggagg cgcgggtcgt gcgcgacgga
     3421 gtcgagacga cggtggctgc caactcgctg gagccgggcg acctcgtccg cgtacgaccc
     3481 ggcgagcggt tgccagccga cggcgaggtc gtcgaggggt tctccgccat cgaccaggcg
     3541 ccgatcaccg gcgagtcgat ggcggtggag cgagaggcgg gcgacgaagt ctttgccggc
     3601 acgcttaacg gctcgggcgt gctcgacata cgggtcacga agcgctcatc acagtcgatg
     3661 ctggccagta tcgtccatct cgtcgaggag gcgcaggcca gccgtgcccc gtcgcagcag
     3721 ctggtcgatc gtttcgccgc cgtctacacg cccatcgtcg tggcgcttgc tgcggtgatt
     3781 gcgctagccg gaggcgctct cgccggcgac tgggacacgt gggtctaccg ggcgctggtg
     3841 ttgctcgtga tcgcctgtcc ctgcgcgctc gtgatctcca cgccggtctc gatcgtctcg
     3901 gcggtcggtg ccgccagccg ggccggcatc ctcgtcaagg gcggtgcggc actggagggg
     3961 gccggccgtg caaaggtggt cgccttcgac aagacgggca cactgaccct cgggcgtccg
     4021 gcagtcaccg cggtggtgcc gtatggcgac cacggcgagc aggacttggt gcgcatcgcg
     4081 gctgcggtag agcagggatc tgagcaccca ctcgcccgcg cggtggtcgc cagagcactc
     4141 tacgatgatg ttccgattcc ggcggcgccc ggcttttcgg cgatcccggg ccgcggcgca
     4201 agggcgagcg tcgacggcgc cgagatcgtg gtcggcagcg agcggctgat gcttgagagc
     4261 ggtctgcaag gcggcgctcg ccgttggctt gctgatgtga cgctggccga ggcggcgcag
     4321 ggtcgctccg tgctggccgt cggcacctcg tcaggcgatc tgtacggggc catcgtcatc
     4381 gctgacaaga tccggccgcg cgcgcgcgag gcgctgggtg ctcttcggcg tgtcggcgtc
     4441 gagcgactgg tggtgctcac gggcgaccgc caggaagtgg ccgagcggat cgcggcggag
     4501 gtcggggtcg acgaagtgcg tgccgagctg ttgccggatg agaaggccgc tgtcgtcgcc
     4561 gatctccgga agcggtacgg gccggtagcg atggtcggag acggcatcaa cgacgccccg
     4621 gcgctggcag cggccgatgt cggcatcgcg atgggcgtgg cagggtccga cgtcgcgctg
     4681 gagagcgccg atctggcact gatgcgggat gatctggagg cgacgtcgcg gctgtttggc
     4741 ctttcgaggc gcacggtggc aatcattcgg cagaacgtca ccctctcgct gattaccaaa
     4801 ctactcgcgt tgctgctggg cgtcttcggg ttcgtcagcc tgtggatcgc ggtactggcg
     4861 gacgtcggca cgtcggtggt cgtcacgctc aacggtctcc ggctcgcgcg cgttgagcag
     4921 cgggtgggac agccggtccc acgcgaagag cgccgcgccg aagatgccga tcgccctctg
     4981 gccgctgact gaccgggatg gggagcggag ggcggcgatg ctgccggggg gcgcggcccg
     5041 gtcagggtgc tggcgggcgt gcgtggttga tttcgaaccg cccgacccgg tgcgccaatt
     5101 ctcctcgctc gatgcgttcg caagagaaga gacagcgcac ggatcgcgcg ttcctcgacg
     5161 tactagcggc actgggttat ggaaggctgt caaatagctc gcactcgttg gcaaccttga
     5221 cgtgtccgtc gttccaggca aaaccgagaa ggctgccata cggccatgtt tctgcttggc
     5281 agacttctcc gccgtaccct acgtgtcctc gtcgcgcatg ccctccggca cgtatttgcc
     5341 gtggtcgggg accgcgattt cgtcgaatcg gtcggcgagc agtttcagct tgatcttcag
     5401 ttctggccca tggagcgccg ggccgtgccc ggcaatcgcc agttccggct cgagatcggc
     5461 aagcgtccgc accgactgcc gcgccgatgg ccagtccggc gtgagatacg tcggcgggcc
     5521 ctgcagggcc ggcttctgct tcgcgaccgc gtagatcgac tcctgatcgg ttgtgacgaa
     5581 cgcgtccccg gcgatcattg agcggtcgga ctcccgccaa agggagatat gcccctcggt
     5641 gtggccgggg gtatggagcc agcgccaacc cggcagctgc gggacactgc catccggcgg
     5701 aagagtctgc agccactcct tcacgtcgac cggccccttc gggaagaagc gggagagcgc
     5761 cgccatcatg ccaccgccga cgcttggatc gggggctgga taggcggtag agccgctgag
     5821 gtagggcact tccagcggat gggcgtagat cggcacctcc caatcggagg cgaggctctt
     5881 gagcgcgccg acgtgatcga aatgaccgtg agtcatgatg atggctgctg gcttggcgcc
     5941 cttgccgaag cgctcctcca ccgctttgcg gatgaagcgc gcggtgcccg ggatgccggc
     6001 gtcgaccagc acccagtcga cgctgcctgg catgccgatg tagacggcgt tgacgatgcc
     6061 gagatggtgg taggcaatgt cgtctgcgat tgtggtagct ggatcctcgg acgcgatgtt
     6121 ggcaacggcg ttgtcgtcga tcgagatgtg ctcgctcatg gcagaacctc caatgtacta
     6181 cgtcgcgaga gacgtcgtgt cgttgccact gcaaggctgg tgccgggacg tacagagcga
     6241 gcgcaagcgt cagccggtcg tcacgctgag cgaagcgtgg accgggggag gtgtggcgat
     6301 caccccgggt gccattcggc cggtgaagtc acctggaaag ctgcccggac actcctgctg
     6361 gtccctgcgg atcgcttggc aacggcgtcg ccacagcact cacgggagac ggctcgatcg
     6421 tgaatctctc gttgatgtcc gtgaggttcc agaccatgaa gtgcatcttc gcgatcatcg
     6481 ccaggcgcag caggttgccg gtgtccgggt cgacccacca cgcgaaccag gctccactct
     6541 gcccggtttt cgacagcaga tagaaagtca gaatctttgt ttcgacgcca tcgaccgtct
     6601 cggtgccacc ggattgcaca ttggttgccc cggtatagat ctcagcgcgc tcagcgaagg
     6661 tcgctgcctc atcggactcg gggagttcgt aggtgcgcgt ctccgcgacg gtttccccgc
     6721 caccggagag gcgaatgtcg agcgagttgg taccagccgg tttcagggag ctcgattctg
     6781 cctgttgggg gagaagcggg gtgtcatcgg gccttctggt ccgaaggcta ctccttctgt
     6841 cgtgggtcga gcgcatcccg cagcccgtcg ccgacgaggt tgaaggagag tacggtgatg
     6901 gtgattgcga tgccgggcgc gatggcgatg tgcggatagt tgcggatgaa ctcacggcca
     6961 tccgagagca tcgtgcccca cgaggcggtc gggcgcgaga cgccgagccc gaggaacgac
     7021 agcgccgact cgagaatgat aacgcctccg attgcgaggc tggcgatgac tattaccggg
     7081 ccgagtgcgt tcggcacgat gtgccggaag atgatgcggc ggtcgccggc accgatcgct
     7141 cgggcggcga ggacgaagtc gcgctcacgc aggctgagca cctcggcgcg gacaaggcgg
     7201 gcatacgacg cccagacggt cagcccgatg acgatgacga cgttgcggag gctcgggccg
     7261 aaaacggagg agagggtcag cagcagcgcc agcaacggaa aggccatcag ggtgtcgacg
     7321 aagcgcgaga ggatgaagtc gacgcggccc ccgaagtatc cagcggtcgc cccaatcgtg
     7381 acaccgatgg tcacggcgat ggccgtcgcc ccgagcccga cgatcagcgc cacgcgcgcg
     7441 ccgtagatga tgcggctgag aatgtcccgt ccgatgtcgt cgttgccgag caggtactcg
     7501 gccgaaggag ctgctccgcg gagggatgtc tcggtttcgg tcgggctgtg cggagcgatc
     7561 cacggggcga agacggcgag gatgacgagc aggccgatga cagtgaggcc gacaagtccc
     7621 ggcttgtagc gcacgaagcg gcgccagatg cggctgcgct cgctgcgcgc cggcggcatc
     7681 ctgcccatct cctccagcgt cgcggtttcc gtcctggtca tcgcctggct gctcatcgga
     7741 cgcgcaccct cgggtcgatc acggcatagg tcag
//
LOCUS       DASYWL010000347         3498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_225078, whole genome shotgun sequence.
ACCESSION   DASYWL010000347 DASYWL010000000
VERSION     DASYWL010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225078"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VGR22_12180"
     CDS             complement(<1..362)
                     /locus_tag="VGR22_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129366.1"
                     /translation="MSTARIIIDRDYRIGEIDPRLYGSFIEHLGRAVYGGIYEPNHPT
                     ADEQGFRQDVLDVVRELGVPYIRYPGGNFVSGYNWEDGVGPVEDRPRRLDAAWKTTEP
                     NLVGTNEFAAWAQKAGAEV"
     gene            complement(978..2189)
                     /locus_tag="VGR22_12185"
     CDS             complement(978..2189)
                     /locus_tag="VGR22_12185"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV2129367.1"
                     /translation="MRTSAHSRYWLVRVLVLGMLMLGLNPVLGFIGGAAAQEGEQETP
                     GPVVNVELVFDSSGSMAQEVAPGVTRIEAAKSVLNDVIDAMPEREGVNVGFRVYGHEG
                     SNQEADREESCQSTDLVVPIEGVNKEALREAVARYEPVGWTPITLALQEAAEDFSAPQ
                     ENERNAVVLVTDGLETCGGDPCAAAEALAQGDVSVTTYVIGFALAEGEQETLQCIADA
                     GGGLNLGAGSAEELNRALFTVLEELQVVVQTGFLEIEAFGDVFPRATIVCRAGATDSN
                     PEGEEQTVVLTDSNRVELNVGLCEVSWVNPSGSETEILVNILPDETTWVRGSLLKFPQ
                     GAGEIYIVTDLGGTVIWQDQFEQGDFVWVLPGIYRMELLERVGDPILISAEVQTLPGT
                     VTQLEIFTAGS"
     gene            2409..>3498
                     /locus_tag="VGR22_12190"
     CDS             2409..>3498
                     /locus_tag="VGR22_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129368.1"
                     /translation="MTRLMVVLAILAGLVPSAGSLAWAQGEQPPGQQAPAVDEIVIGD
                     ERYQFDRVVPVDLGQLQQVAETANGTPVYAKGEADPLGAVYVPSDTGGARRYLPEHLT
                     APASSCAVATVDASTIEGAGFVYVPTGIEPDLTTDQLVEVGTTEDGRTLYASSAEQPF
                     DVLFATGAEGGLVRYIAIGENGVPTIFPDRMIFAGQEFQLTAGETPDPSGLQRGGCAL
                     RFPALAAAGESPGSFSAVHLRVGDSLVTYQSVGPAEAPTATPTEPAVTPTETPEPTAT
                     ETPEPTATPEPTATPTPTPEPTATSTATPEPTATPTGTSSPTATPTETPTPTPEPTET
                     PEPTETPVPTETPEPTAVPTEAPTSTPEL"
BASE COUNT          646 a         1226 c         1044 g          582 t
ORIGIN      
        1 acctcggcac cggccttttg cgcccacgcg gcgaactcgt tagtgccaac caggttcggc
       61 tcggtcgtct tccaggccgc atcgagacga cgggggcggt cctcgaccgg gccgacacca
      121 tcctcccagt tgtagcccga gacgaagttg ccgccgggat agcggatgta cggcacgccg
      181 agctcgcgaa caacgtccag cacgtcctgc cggaagccct gctcgtcggc ggtggggtgg
      241 ttgggctcgt agatgccgcc gtagaccgcc cggcccaggt gctcgatgaa cgagccgtag
      301 agccgagggt cgatctcgcc gatccggtaa tccctgtcaa tgatgatccg cgccgtgctc
      361 aagatatttg cctcccctgc tcacctaagg cagcatggtc gcatgtgctg tacgtggtgg
      421 ctacaaggga cattggcctc gaagaagacc gcacctcggg aggtgtgacg cggcccagcc
      481 accctccgtt ggcgtaccgg tagaagtcgt cccgcggatc gactgacagg tccatgtcgg
      541 cagggtcgat gccatgcacc tccccatctg atcgccggaa gaccgccagg gcagcgttcg
      601 ccactgcaag acccatcatc atgctcgccg ggagaccgac cgccacccca agaaactgcc
      661 gacgattcaa tcgcatctgt cgctcctcga acgttcttgc agtggttcgc cgcgcgcagc
      721 aggtaccggc acgcctgctc tcacgtgagc acgcctgtct tccacggctg cctggttatg
      781 tctctgactc gaaaacgaat tcccgttggt gttttcgcag attcccttcg cggagtccta
      841 tgccggatcc tccagtcgcg caacggttgc gcgcttttgg caatcgacgc aggagcgagt
      901 gacgttgtac atgaagaagc cgcacccgga agtggttcca gatgcggcct gagcgaggtt
      961 cgaattgtgg tgggtcacta tgacccggcg gtgaaaatct ccagttgcgt gaccgtgcct
     1021 ggcagggtct ggacctcggc cgatatcaga atcggatcac cgactcgctc cagcagctcc
     1081 atgcggtaga tccctggcag cacccacacg aaatcaccct gctcgaactg atcctgccag
     1141 atcacggtcc cgcccagatc cgtgacgata tagatctcgc cggccccctg cgggaacttg
     1201 agcaatgaac ctctgaccca cgtcgtctcg tcaggcagga tgttgacaag aatctccgtc
     1261 tcgctgccgc tcgggttcac ccagctgacc tcgcagaggc cgacgttgag ctcaacgcga
     1321 ttggagtcgg tgagcaccac cgtctgctcc tcaccctcgg gattggaatc ggtcgcgccg
     1381 gcgcggcaga cgatggtcgc gcgcgggaag acgtcgccga aggcctcgat ttcgaggaag
     1441 cctgtctgca cgacgacctg cagctcctcc agcactgtaa agagtgcccg gttgagctcc
     1501 tcggcactgc cggccccgag gttgagacca ccaccggcat cagcgatgca ctggagcgtc
     1561 tcctgctcgc cctcggccag agcgaaaccg atgacatacg tcgttacgct gacatcaccc
     1621 tgggcaagcg cttcggccgc cgcacaggga tcgccaccac acgtctcgag gccgtcggtg
     1681 acgagcacga cggcgttgcg ctcgttctcc tgcggcgcgc tgaagtcctc tgccgcttcc
     1741 tgaagcgcga gcgtgatcgg cgtccagcca accggttcgt aacgtgcgac ggcctcgcgc
     1801 agcgcctcct tgttgacccc ctcgatcggc accaccagat cggtcgactg gcagctctcc
     1861 tcgcggtcag cctcctggtt gctgccctcg tgcccataga cgcggaagcc gacgttcacc
     1921 ccttcccgct cgggcatcgc gtcgatcaca tcattgagga cgctcttggc ggcctcgatg
     1981 cgcgtcacgc cgggagcgac ctcctgggcc atcgagccgg acgagtcgaa aaccagctcg
     2041 acattgacga ccggcccggg tgtctcctgc tccccctcct gcgcggctgc gccgccgatg
     2101 aatcccaaca cgggattgag ccctagcatg agcatgccga gaacaagcac ccggacgagc
     2161 cagtaccgcg agtgcgccga cgttcgcatc gtttccctct cctcgtgagc tgaccggagc
     2221 cgtgctgtct gtcgcagatg gaccgcgaca gccataggtt ttgcaactct cgacagccta
     2281 cccgaaaatg cgcgaagctg cacatcgtgt attgccagtc caagtagcgt aatatctgcg
     2341 gtaccaggca ggcatcagcg aacgttcaga ggcttcacgc agcggaggtg gcggaaatgc
     2401 gatccgggtt gacgcgcttg atggtggtgc tggcgatact ggccggtctc gtaccgagcg
     2461 cgggctcgct cgcgtgggcg cagggcgagc aacctccagg ccagcaggca ccggcggtcg
     2521 acgagatcgt catcggcgac gagcgctacc agttcgaccg cgtcgtgccg gttgacctcg
     2581 gacagctgca gcaggtcgcc gagacagcaa acggcacgcc ggtctacgcg aagggagagg
     2641 cagacccgct cggtgccgtc tacgtaccga gcgacaccgg aggagcgcgc cgctacctgc
     2701 cggaacacct caccgctccg gccagcagct gcgccgtcgc cacggtcgac gccagcacca
     2761 tcgaaggcgc ggggttcgtc tacgtaccga ccggcatcga accagaccta accaccgacc
     2821 agcttgtcga ggtcggcacg acagaggatg gacgcacgct gtacgcgtca tctgccgagc
     2881 agcccttcga cgtgctcttt gccaccggag cggagggcgg cctcgtgcgc tacattgcga
     2941 tcggcgagaa tggagtgccc accatcttcc ccgaccgcat gatctttgcc ggacaggagt
     3001 ttcagctcac cgccggcgag acaccggatc ccagcggcct ccaacgaggt ggctgcgcgc
     3061 ttcgcttccc ggcgctggct gccgctggcg agtctcccgg ctcgttcagt gccgtgcacc
     3121 ttcgggttgg cgactcgctg gtgacctacc agtcagtcgg acccgccgag gctcccaccg
     3181 cgacaccaac cgagccagcc gttacaccga cggagactcc cgaaccgacc gcgaccgaaa
     3241 cgcccgagcc caccgcgacg ccggagccga ccgcaacccc aaccccgaca cctgagccca
     3301 ctgcgacctc gacggccacg cctgaaccaa ccgccacacc gaccgggacg tcatccccta
     3361 ctgccacacc gaccgaaaca ccgactccca cacctgagcc aacggaaaca ccggagccca
     3421 ctgagactcc tgtcccgacc gagacgccgg agccaacggc ggtgccgacg gaagcaccta
     3481 cgtcgacgcc cgagctca
//
LOCUS       DASYWL010000348         7744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_112591, whole genome shotgun sequence.
ACCESSION   DASYWL010000348 DASYWL010000000
VERSION     DASYWL010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7744
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112591"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..1267
                     /locus_tag="VGR22_12195"
     CDS             149..1267
                     /locus_tag="VGR22_12195"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEV2129369.1"
                     /translation="MGAIATQLETTVVRESADTLRLYMATLPGPGQVEWAPYELGEVI
                     DNAGLHYLVEAPSVPADVRASLDAGLTVEKFAPAPAETAAQPDDLSELAESDLGGLRN
                     QVDSDRIASLIAEFQAMGAADGGSVGTRHYTTIGSQIAAEFLYRELESYGFDVWYEEF
                     LTWQGYLVVNVVGELPGRDTGRLYGVMAHFDSISDSPTVAAPGADDNATGMASALEIA
                     RVLSGYDLAYSLQVAFVNVEEEGIIGAEEYARNSLEAGAPLEGVFNLDSIGSSRHGDQ
                     IMLNATGDSIWMQELIQRVNSGYGIGESILIHQSDEIVADDNRLREEGVEAVLVAREL
                     YGMSPVHHTERDVIENVSMRHTRSATELVLVTVGALLV"
     gene            complement(1309..2445)
                     /locus_tag="VGR22_12200"
     CDS             complement(1309..2445)
                     /locus_tag="VGR22_12200"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HEV2129370.1"
                     /translation="MPPSITPHRNGRGSGSRSRPNFWAVIPAGGSGTRLWPLSRAARP
                     KFLLPLLGERSLLQQTFERLQRVTIPERMLVVCGPAHAAAIARQLPELPSANIIVEPS
                     PNGTGPALALATALIDQMDPGAIMGSFAADHDIGDAAAFERAVKTAIRAARGDVLVTI
                     GLTPTRPDTGYGYIERTDEVVVQHEGQTAYRAARFIEKPDLERATEFVASGRFLWNAG
                     MFVWRVQALKRELRRQQAQIHEGVWEVADAWESREQEHTTARIWSQLPAITIDHGIME
                     HAQRVAVVPAEMGWSDVGDWNGLGELIERDALGNSVRGDLLRIDTTNSVIWSETGRMV
                     AMVGLDNIIVVDTEDALLVVDRAKSQEVKKVVEQLRASAHNELS"
     gene            2557..3657
                     /locus_tag="VGR22_12205"
     CDS             2557..3657
                     /locus_tag="VGR22_12205"
                     /inference="COORDINATES: protein motif:HMM:NF042587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HEV2129371.1"
                     /translation="MAGAAEETQAGGVSVVEPRLDAKPWGGRRLERYGFDLPRGETIG
                     EALVTAGEAMITAGAGTGRSLADIVRTDPEGALGAVAREAVRGRAIFPLLVKLIDAQE
                     NLSIQVHPNDEQAASRDRLGKTEAWHVLTAEPGSSLYLGLQQGVSLEELQAAAQRLDG
                     ASAALLRKVPAVPNTTVLIPAGTVHALGAGVMVYEIQQPSDVTFRMDDWGRVDAQGNP
                     REMHLDEGFVVMNPTLRPELIDPVDLAAREGRRHMLVACRHFALERVALPGGGRYTMP
                     GHASSQVVTVLEGAATVGSERLGAGQSAVMWPGRMETVLEAVRPMVALRGWVPDLAAD
                     FPVLTRRAAIDVSTLATLSGLLPDVRAALELQ"
     gene            3673..4599
                     /locus_tag="VGR22_12210"
     CDS             3673..4599
                     /locus_tag="VGR22_12210"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEV2129372.1"
                     /translation="MTSLRARYVGTLLGLAAGDALGGSVEFMPRGAIQRAYPGGLREI
                     TGGGSHDLRLGEVTDDTAMALAIARACTPDGIDLDQVAENFLTWYRSDPKDIGITTSR
                     ALAKMDAGVPWPEIGERMMAETPRKLAGNGSVMRCAPVALRFRHDREQLREVSLDTAR
                     MTHPDPMATWGGVALNQAIAYLLDGGDRDGVRDAATADIENAEVAEAIRMAADREYDD
                     VRSGGFVLETLGAAFWALARSDSAEEAIVTAVNMGDDTDTTGAVTGALVGAHYGVDAL
                     PDRWLVVLEPREELIVLAERLLEWSEADAARS"
     gene            complement(4727..5851)
                     /locus_tag="VGR22_12215"
     CDS             complement(4727..5851)
                     /locus_tag="VGR22_12215"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV2129373.1"
                     /translation="MTNERARLNKPNRRPTPRDGMTSEERDRTLEDPSLEALERRTQP
                     EKPNQLALFAEGPAGTFTASPSGLAPLTASSSLALARAWYHRELEQARRPKNTIAAYT
                     NDLAVLEHLIGSKSIAAVTRRDIARYLGDANSRTTRKRRLTSARRFFDFLIEQAKVLK
                     ADPTDGFYPHGIQLRSPVPLFPDEQERLLAAAEDDAPWSLPAIWLMMRFGLTRAELLT
                     LERDHIDRTTEPAPTVYVFYADPTKRGKERHLLTTPEFGDVLDQFLEARNPHGRLFPV
                     GPQAVNGMVERVRRAAEIDKEVTPNVLRHTFAVDQARQGADQQRLLALLGLADDPRNR
                     ASVDRYLKLAQPPVNAADQPDAGTSADVADPPNEDTPGQT"
     gene            6114..7292
                     /locus_tag="VGR22_12220"
     CDS             6114..7292
                     /locus_tag="VGR22_12220"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV2129374.1"
                     /translation="MGELIVGRPALQVKAAVSLPLDLVSVMSLLYRAVPGSGLDAWLV
                     STRRGLPAQLQHDLDLLHGFSGRLLYFMEEPIMRFEPLREDRLHASVGDLLAFLEVLP
                     AETYREMLEHAVGRVHQDLGTGLVAPQGDHELEWRRFIEPGITTACGEEVLKLVLDSI
                     QLKDRTIRLIRGVWEIAFESEYMRFRDSLEAAGRIAQEAAGRGLTAAFGELTGNRVPS
                     TLAAGLGSAARVTFVPSMHLGSFMSYMVYPPDVIIFFSAQRVLDGDVQQVGVASSRNG
                     RVESMSAGELEAGDVLEGTELLDALRALGDANRLRILDLLGTGELYANEIVGRLGIAQ
                     SAVSRHLSQLERAGLVTVNPRGGMKYYAIDANRLGALAETLRVLAVQAGAADGTEVAS
                     "
BASE COUNT         1267 a         2364 c         2739 g         1374 t
ORIGIN      
        1 ccgagaggat ttggctgcct tgtgcagcga ggcctgtttg gccagggtcg agcgttcttc
       61 ggaggcggcg cgggtactgg gatcgactgg cactcgccct tcttttgccg gagaggactg
      121 gctttggttc atcgcccagc cggagcagat gggcgccatc gcaacccagc tggagaccac
      181 cgtggtgcgc gagagtgcgg acacgctgcg gctctacatg gcgaccctgc cgggcccggg
      241 tcaggtggag tgggctccgt atgagctcgg tgaggtgatc gacaatgctg gtcttcacta
      301 cctcgtcgag gctccatcgg tgccggcgga tgtccgtgcc tcgctcgatg ccgggctgac
      361 agtcgagaaa ttcgcgcccg ccccagcgga aacggcggct cagcctgatg acctatccga
      421 gttggcggaa agcgatctcg gggggctgcg gaaccaggtg gacagcgatc gcatcgcatc
      481 gctgatcgcc gagtttcagg ctatgggtgc cgccgacggc ggcagtgttg gcaccagaca
      541 ctacaccacg attggcagcc agatcgcggc cgagttcctc tatcgggagc tggaatcgta
      601 cggcttcgac gtctggtacg aggagttcct cacgtggcag ggatatctcg tcgtcaacgt
      661 ggtgggcgag cttccgggac gggacaccgg ccggctctac ggcgtgatgg cgcacttcga
      721 ctccatatcc gactcgccga cagtggccgc accgggagcc gacgacaacg ccaccggcat
      781 ggccagcgcg ctcgagatcg cccgcgtcct gagtggttac gaccttgcct actcgcttca
      841 ggtggcgttc gtcaacgtcg aggaggaggg gattatcggg gccgaggagt acgcacggaa
      901 ctcgctcgag gcgggagcgc cgttggaggg ggttttcaac ctcgactcca ttggctcatc
      961 tcgtcatggt gaccagatca tgctcaacgc gacgggtgat tcgatctgga tgcaggagtt
     1021 gatccagcgg gtcaacagcg gatacggcat cggcgagtcc atcctgattc accagagcga
     1081 cgagatcgtt gccgacgaca atcggttgcg ggaagagggg gtggaggccg tgctagtcgc
     1141 acgggagctc tatggcatga gcccggttca ccacactgag cgtgacgtca tcgagaacgt
     1201 ctccatgcgg cacacccgct cggccaccga gctcgtcctc gttactgtcg gagccctgct
     1261 cgtgtagcgg caccacctgc acggaattcc ggcacagacc gccccacctc aactcagctc
     1321 gttgtgagcg gatgcgcgaa gctgctcgac gaccttcttg acctcctgtg acttggcacg
     1381 gtcgacgacg agcagcgcgt cctcggtgtc gacgacgatg atgttgtcga gccccaccat
     1441 ggcgaccatg cgcccggttt ccgaccagat cacgctgttg gtcgtgtcga tgcgcagcag
     1501 gtcgccccgc accgagttgc ccagcgcgtc ccgctcgatg agctcgccaa gaccattcca
     1561 gtcgccgaca tccgaccagc ccatctcagc cggcaccacc gcgacccgct gggcgtgctc
     1621 catgatgccg tggtcgatgg tgatggccgg cagctgggac cagatgcggg cggtcgtgtg
     1681 ctcctgctcg cgcgactccc aggcgtccgc cacctcccaa accccctcgt ggatctgcgc
     1741 ctgctggcgg cgcagctcgc gcttgagggc ctgtacccgc cagacgaaca tgcccgcgtt
     1801 ccagaggaat ctgcccgacg cgacaaactc ggtcgcgcgc tccagatcgg gcttctcgat
     1861 gaaccgtgcg gctcggtagg cggtctggcc ctcatgctgc accacaacct cgtcggtgcg
     1921 ctcgatgtag ccgtagccgg tgtcgggccg ggttggggtc aggccgatcg tgaccagaac
     1981 gtcgccgcgt gctgcccgga tggcggtctt gacggctcgc tcgaaggcgg cggcgtcgcc
     2041 gatgtcatga tcggcagcaa agctgcccat gatcgctccc gggtccattt ggtcgatcaa
     2101 ggccgtcgct agcgccagcg ccgggccggt gccgttgggg gacggctcga cgatgatgtt
     2161 ggcactcggc agctcgggca gctggcgggc gatcgccgct gcatgggcgg ggccgcacac
     2221 aacgagcatc cgctccggga tcgtcactcg ctgcaggcgc tcgaaggtct gctgcagcag
     2281 cgaccgctcg ccgagcagcg gcagcaggaa tttcggccgt gccgcacggc tgagcggcca
     2341 gaggcgggtg ccggagccac cggccgggat gacggcccag aagttggggc gtgatcggga
     2401 tcccgatccc cggccgttcc gatgtggcgt aatcgatggc ggcacaatct gctccttggc
     2461 gtcacaacgg ggactcgtgg aagtatagtg ccggagagat agccgatgtc gagatgtggc
     2521 ctgtcgggat ggcgggtgca gcagggtggg ctggcgatgg caggtgcagc agaggagacg
     2581 caggctggcg gggtctcggt ggtggagccc cggctcgatg ccaagccgtg gggtggccgg
     2641 cggctggagc gctacgggtt cgatctgcct cgtggcgaga cgatcgggga ggcactggtg
     2701 acggctggtg aggcgatgat caccgccggc gcgggcaccg ggaggagcct ggcggatatc
     2761 gtgcggaccg acccggaggg agcgctcggc gcagtcgcgc gggaggcggt ccggggcagg
     2821 gcgatctttc ccctgctcgt gaagctgatc gacgcgcagg agaatctttc cattcaggtc
     2881 cacccgaacg acgagcaggc tgcctcgcgc gaccgcctcg gcaagaccga agcatggcac
     2941 gtgctgaccg cagagcccgg cagcagcctc tacctcggcc tgcagcaggg cgtctcgctg
     3001 gaggagctcc aggcggccgc tcaacgtctc gatggagcct cggcggcgtt gctgcggaag
     3061 gtaccggcgg taccgaacac gacggtgctg atcccggccg gcacggtgca tgcgctgggt
     3121 gccggggtga tggtctacga aatccagcaa ccgtccgacg tgaccttccg gatggacgat
     3181 tgggggcgcg tcgatgcgca gggtaacccg cgcgagatgc accttgacga gggcttcgtg
     3241 gtgatgaacc cgacgttgcg tccggagttg atcgacccgg tcgatctcgc cgcgcgcgag
     3301 ggacgccggc acatgctggt tgcctgccgc cacttcgcgc tggagcgcgt tgcgctgccc
     3361 gggggtggcc ggtatacgat gccgggacac gccagctcac aggtggtgac cgtcctcgag
     3421 ggtgcggcaa cggtcggatc ggagcgtctc ggagcgggac agtctgcagt catgtggccc
     3481 ggccgtatgg aaacggtgct cgaggcagtc cgcccgatgg tcgcgcttcg tgggtgggtc
     3541 cccgatctgg cagccgattt ccctgtgctc actcgccgcg cggcgatcga cgtctcgacg
     3601 ctcgcgaccc tcagcggcct gctccccgat gtgcgcgccg cacttgagct tcagtaggga
     3661 aggaacgaga ccgtgacatc actgcgcgcg cgctacgtgg gaaccctgct cggcttggca
     3721 gccggagatg cgctgggcgg gtcagtcgag ttcatgccgc gtggggcgat ccagcgagcg
     3781 tatcctggcg ggctcaggga gatcaccggc ggtgggtccc acgacctgcg gctgggcgag
     3841 gtgaccgacg atacggcgat ggcgctggca atcgcgcggg catgcacgcc ggacggtatc
     3901 gatctcgatc aggtcgccga gaacttcctt acctggtacc gatcggatcc gaaggacatc
     3961 ggcatcacga ccagccgcgc actggccaag atggacgccg gggtgccatg gcccgagatc
     4021 ggcgagcgga tgatggccga gacgccgcgc aagctcgccg gtaacggttc cgtgatgcga
     4081 tgcgccccgg tcgcgctgcg gtttcgacac gaccgcgagc agttgcgcga ggtcagcctc
     4141 gacactgcac ggatgacgca tccggacccg atggcgacgt ggggaggtgt cgcgctcaac
     4201 caggcgatcg cctacctgct tgacggtggc gaccgggatg gcgtacggga tgcggcgacc
     4261 gccgatatcg agaacgccga ggtggcggag gcgattcgca tggccgccga tcgggagtac
     4321 gacgacgtgc gctcgggcgg gttcgtgctg gagacgctgg gtgcggcgtt ctgggcgctc
     4381 gcgcgcagcg attccgccga ggaagcgatt gttacggcgg tgaacatggg cgacgacacc
     4441 gacaccactg gcgcggtcac cggtgcgctg gtcggcgcgc actatggcgt cgatgccttg
     4501 cccgaccgct ggctggtggt gctggagccg cgcgaagagt tgatcgtcct cgccgaacgg
     4561 ctcctcgaat ggagtgaagc cgacgcggcg aggtcctgat ggttccgccg gatgggtaga
     4621 ccgcgtggag ccttgtggga gcgaagccta ccctacccct acgggcgttc gctcttccct
     4681 gctggttttg ccgacggatc actcgatatt cgcactgcgc tgcgcgttac gtctggccgg
     4741 gagtgtcctc gttgggagga tcggcgacgt cggcgctcgt accagcgtcc ggctggtcag
     4801 cggcgttgac cggcggctgg gccagcttga ggtagcgatc gacgctggcg cgattgcgcg
     4861 ggtcgtcggc gaggcccagc agggccagca accgctgctg gtcggcgccc tggcgagcct
     4921 gatcgacggc gaaggtgtgc cgcaacacgt tcggggtcac ctccttgtcg atctcagccg
     4981 cgcggcgcac gcgttccacc ataccgttca cggcctgtgg accaacaggg aagaggcggc
     5041 cgtgcgggtt gcgcgcttcc aggaactgat cgagcacgtc gccgaactcg ggagtggtca
     5101 ggaggtgtcg ctccttgccg cgcttggtgg ggtcggcgta gaagacgtag acggttggcg
     5161 ccggctccgt ggtccggtcg atgtggtcgc gctccaatgt cagcaactcg gcgcgggtga
     5221 gaccgaaccg catcatcagc cagattgccg gcaggctcca gggcgcgtca tcctcggcag
     5281 ccgccagcag gcgctcctgc tcgtcaggga agaggggcac cggagaccgg agttggatgc
     5341 catgcgggta gaacccgtcg gtcggatccg ccttgagcac cttggcctgc tcgatgagaa
     5401 agtcgaagaa gcggcgtgcc gaggtgaggc ggcgctttcg ggtcgtgcgg ctgttggcgt
     5461 cgccgagata gcgggcgatg tcgcgccggg tgacggcggc gatcgacttg gagccgatca
     5521 ggtgctccag cacggcgagg tcgttggtgt aggcggcgat cgtgttcttc gggcggcgcg
     5581 cttgctcgag ttcccggtgg taccaggctc gggcgagggc gaggctcgag cttgccgtga
     5641 ggggcgcgag gccgctcggt gatgcggtga aggtgccggc cgggccttcg gcgaagagtg
     5701 cgagctggtt cggcttttcc ggctgcgtgc gccgctcgag cgcctcgagc gacgggtcct
     5761 ccagagtgcg gtcgcgctcc tcgctcgtca tcccgtcgcg cggggtgggc cggcggttcg
     5821 gcttgttgag tcgggctcgc tcgttggtca ccgtcgcatc ccctcaaggt cggaactatc
     5881 gtgccgggat gacgctttgg gcagcgtacg tttcgctgaa cataggtgtc aagtagacgg
     5941 aacggctgct gagaatcata taccaacgta tattgaccct tccgagctat gctgatatcg
     6001 tgatcgaaac atgtaagggt ggtcaaggtg gggccggatg agattgcttc aaggagcgca
     6061 cgcccatcta tcgtgatggg tgctggccaa ctgtacaggg agtcaacaca gcaatgggag
     6121 agctcatcgt cggacgtcca gcgctccagg tcaaggctgc ggtttcgctg cccctcgact
     6181 tggtgtcggt gatgtcgctg ttgtaccgtg ccgtgccggg cagtgggctc gacgcctggc
     6241 tggtatcgac gcggagaggg ctgccggcac aactccagca tgacctggac ctgctccacg
     6301 gcttttcggg gcgcctgctg tacttcatgg aagagccgat catgcgcttc gagccgctcc
     6361 gggaggatcg gctgcacgcc tcagttggcg acctgctcgc tttcctcgag gttctgccgg
     6421 cggagaccta ccgggagatg ctcgagcatg ccgtcggacg ggtgcatcag gatctcggca
     6481 ccggtctggt cgcgccgcaa ggtgaccacg agctggagtg gcgccgattc atcgagccgg
     6541 gcatcaccac ggcgtgcggc gaggaagtgc tcaagctggt cctggacagc atccagctca
     6601 aggatcgcac gatccggctg atccggggtg tctgggagat cgcgtttgag agcgagtaca
     6661 tgcggttccg cgacagcttg gaggcagccg ggcgaatcgc ccaggaggcg gcgggccgcg
     6721 ggttaaccgc tgcgttcggc gagctaactg gcaaccgtgt gccgtcgacc cttgcggctg
     6781 gcctcggttc cgccgcgcgc gtcacattcg ttccgtccat gcacctcggc tcattcatga
     6841 gctacatggt ctacccgccg gatgtcatca tcttcttcag tgcacagcga gtgctcgacg
     6901 gggacgtgca gcaggtaggc gttgccagca gcagaaacgg tcgtgtcgaa agcatgtctg
     6961 ctggggagct cgaagcggga gacgtgctgg agggcaccga gttgctagac gcgctccggg
     7021 cgctgggcga cgccaatcgg ttgcggattc tcgatctgct cggcaccggc gagctctatg
     7081 ccaacgagat cgtggggcgt ctcggaatcg cgcagtcggc cgtgtcgcgg catctctccc
     7141 agctggagcg agcaggtctt gtgaccgtca acccgcgcgg cgggatgaag tactacgcca
     7201 tcgacgccaa ccggctgggg gcgctcgccg agacactgcg cgtgctcgcc gttcaggccg
     7261 gtgcggcgga tggcacggaa gttgcgtcgt aagtgatcaa acgagatcgg gttcacgaga
     7321 tgggcatatg ctgcactgga caactcgata tcgcgatgat aggatatctc cagacgagag
     7381 aagcgaagac tcgctagcag agctgacacc cgcctcgtca tgaacgttga caacacaccg
     7441 cgaggaagta agggagccct tgacaggact cccaccggag cacgacgtca caccagacgg
     7501 cattcttcca gactcgtcgt gatccgcaga tgacttagcc gcctcgggcg acgttagcca
     7561 gtcgcctggc ataaaacaac cctcttcttt ccttcctttt agtatgccga cgtctgaagg
     7621 agacagatga tgttggcatc acttcaacac tttcctctcc gaagcctgat ctctcggtcg
     7681 cgatcaggcg ggtatccctc ggctgcgacc gaacagaaaa cggaaacgcc cggtgcatac
     7741 gcac
//
LOCUS       DASYWL010000349         2893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_487412, whole genome shotgun sequence.
ACCESSION   DASYWL010000349 DASYWL010000000
VERSION     DASYWL010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487412"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VGR22_12225"
     CDS             complement(<1..393)
                     /locus_tag="VGR22_12225"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV2129375.1"
                     /translation="MRFGIFSFGRIPYRALADRVRLAEDLGFASAWVDDDLLTPRYSQ
                     FEPWTLLGALARDTSTIRLGTMVTVITFRHPSLLAAQVVTLDHLSDGRVEIGLGAGGP
                     PNPYRAFSHTDWTPRERAERLEEYVAVLG"
     gene            652..1617
                     /locus_tag="VGR22_12230"
     CDS             652..1617
                     /locus_tag="VGR22_12230"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV2129376.1"
                     /translation="MASMHVGIMNFTTPPFEELAKRVRDAEELGFSSAWVNDDVLSLS
                     PRGPELEAWTVLTGLARETSRIRLGTLVTVITFRHPALLAAQVITLDHLSQGRAALGL
                     GTGGPPNNYGALGLEAWPPRERAERLEEQAAILDPLLRGETVDFEGQHYQAHEPAVPM
                     PVQRPRPPLIVAAHGDRGLQVVARHADGWNSFGGQPYRVSQDPTKRLSLADAVAETRR
                     LSERLDGYCQDVGRDPATVRRTVLAYRPSVDPLSSLDAFDDYVGRYREIGIDEVIFYW
                     PPLDNLYPRRPGSPEEQPVFDPPRPISPAQQAAFERIATERIASG"
     gene            2051..2404
                     /locus_tag="VGR22_12235"
     CDS             2051..2404
                     /locus_tag="VGR22_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129377.1"
                     /translation="MSHGPGELLREIDPRGRLVRLTSDQWHSHILPRHPDIAGYLSAI
                     PAVIREPEVVRRDAVQWQRECYYRQQDRPAARSLLIKVVVDFSPQEEPDGFVITCFFT
                     TRMRPGEEVLWLPST"
     gene            2386..2826
                     /locus_tag="VGR22_12240"
     CDS             2386..2826
                     /locus_tag="VGR22_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129378.1"
                     /translation="MVAFHLNDASLDTPASPPAHLHLPDAEGAGVTLTYDDIIDSLMI
                     LVDETPGARVVGPVNRYASLLLNERDEVVGAMVDAFLTHAVHERPELQAIAGYMRLGS
                     RPHGDTARFRTDRPERSAQPDEAWRDAAVRAVRELFAITGGYRE"
BASE COUNT          509 a          964 c          935 g          485 t
ORIGIN      
        1 accgagtacg gcgacatact cctccagccg ctcggcccgc tcgcgcggcg tccagtccgt
       61 gtgactgaag gctcggtatg ggttcggtgg gcccccggcg ccaagcccga tctccactcg
      121 accatccgaa aggtgatcga gggtcacaac ctgggcggcc aggagcgatg ggtggcggaa
      181 ggtaatcacg gtgaccatgg tgcccaaccg gatggtcgat gtgtccctgg caagggcgcc
      241 gagcaaggtc cacggctcga actgggaata gcgcggggtg agcaggtcgt cgtccaccca
      301 ggcgctggca aatcccaggt cttccgcaag cctgacccga tctgcaaggg cacggtaggg
      361 gatgcggccg aagctgaaaa taccgaatcg catgatgtcc tcctgatgcc gcctttctgg
      421 cagcgtcgac tcggctggca tgccaggtac cgcgctggat gccggacagg ttgggcggtg
      481 cgcaccactg cgaaggcacc ggctcaccct tggaatcgat gaccctcatg atatgatcgg
      541 agaggattcc attgttatgg caattatcaa cccatcgtgg cagaaagacc agcttctgga
      601 ttccatcgct cgtggagcgg tcggcgaccg aaactcacag ggagaacggc aatggcttcg
      661 atgcacgttg gaatcatgaa cttcaccacg ccaccattcg aggagctcgc taagcgcgtc
      721 cgcgacgctg aggagctggg cttctccagc gcctgggtca acgacgatgt cctctcgttg
      781 tctcctcgcg ggcccgagct ggaggcatgg actgtcctca ccggcctcgc gcgcgaaacc
      841 agccgcatcc ggctcggcac cctcgtcacg gtcatcacct tccggcaccc cgccctcctc
      901 gccgcccagg tcatcaccct cgaccatctc tcgcaggggc gcgccgccct cggcttgggc
      961 accggcgggc caccgaacaa ctacggggcg cttgggctcg aggcgtggcc gccgcgggag
     1021 cgtgccgagc gtttggagga gcaggcagcc attctcgatc ccctgctgcg cggcgagacg
     1081 gtcgacttcg aggggcagca ctaccaggcc cacgaaccgg cggtccccat gccggtgcag
     1141 cgtccgcgac cacccctgat cgtggcggcc catggtgacc ggggtttgca ggtggtggcg
     1201 aggcacgccg acggctggaa cagcttcggt ggccaaccgt accgagtctc gcaggacccg
     1261 accaaacgcc tgagcctggc cgatgcggtt gccgaaaccc ggcgcctgag cgagcggctg
     1321 gatgggtact gccaggacgt ggggcgcgac ccggccaccg tgcggcggac ggtgctggca
     1381 taccgcccga gcgtagaccc gctctcgtcg ctcgacgcct tcgacgatta cgtggggcga
     1441 taccgagaga tcggcatcga cgaggtgatc ttctactggc cgccgctcga taacctctac
     1501 ccgcggcgtc ccggctcgcc ggaagagcag ccggtcttcg atccacctcg accgatctca
     1561 ccagcacagc aggccgcgtt cgaacgcatc gcgacggaac gaatcgcgag cgggtgagcg
     1621 gaaacgcact acctcaggtg ttttgccgga tgatcggcca cagaagcctt ggacggcggc
     1681 tgaaaggact catgcaacgg ggctggccag ccgacgagac tcccgccgag gtcgaggagg
     1741 atgtcatcct ctcgcggctg ccgtgctgaa cggggcgcgg gcggcgaggc aatgatgcgg
     1801 gacggggaac gaccactgcc ccgcaggcat ccatcggggg tctctgtgaa aagcgcgggt
     1861 agccattctc ccgccacatt gaccattcct cactcaacga tactccagat gttagccggt
     1921 cctgcgaaga gttcgtcatc cgttcgtggc tgctggtcat gaccggtgcg tggattgcgg
     1981 agaaggtcca tgctgccgtt acatccggca cacggccacc gtcatccttc aacgaggagc
     2041 catagcgttc atgtcgcacg gtccgggtga actcctgcgt gagatcgatc ctcgtggcag
     2101 actcgtgcgg ctcacctctg accagtggca cagccacatc cttcctcggc atccggatat
     2161 cgctggatac ctgtctgcca tcccggcggt gatccgggag ccggaagttg tgcgacgtga
     2221 cgccgtgcag tggcagcggg agtgctacta tcgtcaacag gacaggcccg cagcacggtc
     2281 cttgctcatc aaggtggtgg ttgacttttc gccgcaagag gagcctgatg ggttcgtgat
     2341 cacctgcttc ttcacgacac gtatgcggcc gggagaggag gtcctatggt tgccttccac
     2401 ctgaacgatg cctcgctcga cactccagca tctccgccgg cccacctgca cctgccggat
     2461 gcggaagggg ccggcgtcac cctcacctac gacgacatca tcgacagcct gatgatcctg
     2521 gtcgacgaga cgcccggcgc ccgcgtggtt ggcccggtca acaggtacgc ctcgctgctg
     2581 ctcaacgagc gcgacgaggt cgtcggggcg atggtcgacg ccttcctcac ccatgccgtc
     2641 cacgagcgcc cggagcttca ggcgatcgca ggctacatgc gcctcggatc gcgcccgcac
     2701 ggtgacaccg cccgtttccg caccgaccgc ccggagcgga gcgcccagcc cgacgaggcg
     2761 tggcgcgatg ccgccgtccg cgccgttcgg gagctgttcg cgatcaccgg agggtatcgg
     2821 gagtagctgg cgcaagcgat cagggcgtca tcctctcgcg gctgctggcg ctcaacgccg
     2881 cccacggcaa acc
//
LOCUS       DASYWL010000350         2235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_374723, whole genome shotgun sequence.
ACCESSION   DASYWL010000350 DASYWL010000000
VERSION     DASYWL010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374723"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VGR22_12245"
     CDS             <1..348
                     /locus_tag="VGR22_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129379.1"
                     /translation="ATKIVRRHPHIFGDDAAEHAEEVVGLWQRIKAEERANGKQATKA
                     ADGQPHAMPALERAKRVLKKRPLPEAASDADATGDRLLAVIAGIIDAGEDPDAALRAA
                     LERHVAATNRDNA"
     gene            463..>2235
                     /gene="aspS"
                     /locus_tag="VGR22_12250"
     CDS             463..>2235
                     /gene="aspS"
                     /locus_tag="VGR22_12250"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEV2129380.1"
                     /translation="MPAIDVRPETADRTTCGGLRLENAGERITLQGWVNRRRDLGGLI
                     FIDLRDRFGITQVVFNPEVAKAAHDIAGDLRNEYVIRVEGLVRDRPEGTVNPKMDTGE
                     IELEAHAVEILNPSKTPPFYINEEVDVDESLRLKYRYLDLRRRGMTENMIQRHRVVKH
                     MRDFLDKRGFLEIETPLLIKSTPEGARDYLVPSSQFPGMFYALPQSPQQMKQLLMVSG
                     LDRYFQIARCFRDEPQRADRQPEFTQLDLEMSFVDQEDVMALNEALFIELTEKFSDMT
                     IQEKPFPRLSYREAMDRFGNDRPDLRFGLELQDVSDALRETEFKAFKGVLDNGGQVKG
                     IVVPGCADYTRKQIDELTELAKQAGAKGLATMQLQAGGLKSPTAKFLTQNEVQAMTTG
                     IGAKEGDLVLFVADQPAVVAKALSALREEFGERLQLADANLLAFCWVVEFPLLEWDED
                     VDRWDATHNPFSSYYEEDEHLLDTDPGKVRAKQYDLIGNGFELGGGSIRINKRAKQER
                     IFELMGHSKEAQQERFGALLDALEYGAPPHGGCAFGLDRYAMLIAHEDSIREVIAFPK
                     NQRGVDLMFNAPDTVEPEQLDDLGL"
BASE COUNT          439 a          723 c          720 g          353 t
ORIGIN      
        1 gcgaccaaga tcgttcgccg ccacccgcac atcttcgggg atgatgccgc cgagcacgcc
       61 gaggaggttg tcgggctctg gcagaggatc aaggccgagg agcgcgcgaa cggcaagcag
      121 gccaccaagg cagccgacgg ccaaccccac gcgatgcccg cactggagcg cgcgaagcgg
      181 gtgctgaaga agcggccgct ccccgaagca gcaagcgatg ccgacgccac gggagatcgg
      241 ttgctggccg tcattgcggg tatcattgat gctggcgagg atcccgacgc cgcccttcgt
      301 gctgccctcg aacggcacgt cgcggcgacg aatcgcgaca acgcgtaggg gcggatcccg
      361 atccgcactc aggggcgatc atggaatcgg cccatacggg cgtctccatg atcgcccccg
      421 ttttattcgc gcatcgaatc aaccgtacac cagggagagc ggatgccagc catcgatgtc
      481 cgaccagaaa cagccgatcg taccacctgc ggtgggctcc gccttgagaa cgccggcgag
      541 cggatcacgc tgcagggatg ggtcaaccgt cgccgcgacc tcggtgggct gatcttcatc
      601 gatctccgtg accggttcgg catcacgcag gtcgtcttca accctgaggt ggcgaaggcg
      661 gcgcacgaca tcgccggcga tctccgcaac gagtacgtca tccgggtgga ggggctggtg
      721 cgcgaccgcc cggagggcac cgtcaacccg aagatggaca ccggcgagat cgagctcgag
      781 gcacacgccg tcgaaatcct caacccctcg aagacgccac ccttttacat caacgaagag
      841 gtggacgtcg acgagtcgct gcggctcaag taccgctacc tcgaccttcg ccgccgtggg
      901 atgaccgaga acatgatcca gcggcaccgg gtcgtgaaac acatgcgcga cttcctcgac
      961 aagcgcggct tcctcgagat cgaaacgccg ctgctgatca agagcacgcc cgaaggcgcg
     1021 cgcgactacc tcgtgccaag cagccaattt ccgggcatgt tttacgccct gccgcagtcg
     1081 ccgcagcaga tgaagcagct tttgatggtt tcgggactgg atcgctactt ccagattgcc
     1141 cgctgcttcc gtgacgagcc acaacgcgcc gaccgccagc cggagttcac ccagctcgac
     1201 ctcgagatgt cgttcgtcga ccaggaagac gtgatggcgc tcaacgaggc gctcttcatc
     1261 gagctgaccg agaagttctc ggacatgaca atccaggaga agcccttccc gcgcctgagc
     1321 tatcgtgagg cgatggaccg attcggcaac gaccggccgg acctccgctt cggcctggag
     1381 ttgcaggacg tctccgatgc gctccgggag accgagttca aggccttcaa gggcgtgctc
     1441 gacaacggcg ggcaggtcaa gggcatcgtc gtgccgggct gtgccgacta cacccgcaag
     1501 cagatcgacg agttgactga gctggcgaag caggccggag ccaaggggct cgcgaccatg
     1561 cagctgcagg cgggtgggct caagtcgcct actgccaagt tcctcaccca gaacgaagtc
     1621 caggcgatga ccaccggcat aggcgcgaag gagggcgatc tcgtgctctt cgtcgccgac
     1681 cagcccgccg tggtcgccaa ggcgctctcc gccctgcgcg aagagttcgg tgagcgcctg
     1741 cagttggccg acgcgaacct gctcgcgttc tgctgggtgg tcgagttccc gctgctggag
     1801 tgggacgaag acgtcgaccg ctgggatgcc acccacaatc ccttctcaag ctactatgag
     1861 gaggacgagc atctcctcga caccgacccg ggaaaggtgc gcgcgaagca gtacgacttg
     1921 atcggcaacg ggttcgagct gggtggcggc agtattcgca tcaataaacg cgccaagcag
     1981 gagcgcattt tcgagctgat gggccactcg aaggaggcgc agcaggagcg cttcggcgcg
     2041 ctgctggacg cgctcgagta cggcgcacca ccgcacggcg gctgtgcgtt cgggctggac
     2101 cgctacgcga tgctgattgc ccacgaggac tcgatccgcg aggtgatcgc cttcccgaag
     2161 aaccagcgcg gcgtcgacct gatgttcaac gctccggaca ctgttgagcc tgagcagctc
     2221 gacgatctgg gcctg
//
LOCUS       DASYWL010000351        20501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_299567, whole genome shotgun sequence.
ACCESSION   DASYWL010000351 DASYWL010000000
VERSION     DASYWL010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 20501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20501
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299567"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="VGR22_12255"
     CDS             complement(<1..845)
                     /locus_tag="VGR22_12255"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEV2129381.1"
                     /translation="MTQDWNDSRFETRAIHAGQDPDPATGAVITPIYQTSTYAQEGIG
                     NFKGYEYSRTGNPTRTALQDALASLEGGAWGMAYASGLAATHNLAYLLRPGDHVLLSS
                     DSYGGTFRLFDKVLRNYGIEMSLADLSNLDEARDAVQENTKMVWVESPTNPYLRIVDI
                     AGWAEALKGPDTWLVVDNTFASPYLQRPLSLGADFVLHSTTKYLGGHSDVVGGVAVGN
                     DPEVQQILAFHQNAAGAVPGPMDCWLTLRGIKTLGVRMDRHSANAAKVAEWLQAQDEV
                     ETVYYP"
     gene            complement(1003..2502)
                     /locus_tag="VGR22_12260"
     CDS             complement(1003..2502)
                     /locus_tag="VGR22_12260"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR01137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HEV2129382.1"
                     /translation="MTSLRSARHLTADRARERANGVVKRPDGGVYENVIEALGNTPLV
                     RLNSVTRDIRTPVYAKLEMLNPGGSVKDRIGIRMIEEAEDRGWLRPAGTIIEPTSGNT
                     GVGLAMAAAVRGYRCIFVMPDKVSNEKVQLLRAYGAEVVTTPTSVERDDPKSYYSVAD
                     RLTREIPNSFSPNQYFNQMNPQAHYDATGPEIWNQTEGKITHLVAGVGTGGTVTGIGR
                     YLKEQSAGVVVVGADPEGSIYTTDNIHQYLIEGVGEDFWPGTFDRNIVDEWVQVNDTQ
                     AFTMARRVTREEGILIGGSGGMAVHAAVESARRIDDPDAMVVVVLPDSGRGYLSKIYN
                     DDWMREHGFLARFRGPSDMGSLIDTRERNVPDVIAVACDQTVSQAIALLNEYGVSQLP
                     VVRGSGAQAGKECKGPEPGEEIEVHSVIGSLQERKLLDEIFKDPEVLNKPVREIMEPA
                     FQLIDSHEDIERVIPMLAEKAPAVLVQHEGKLIGIVTRADILNHVAKQR"
     gene            complement(2840..3580)
                     /locus_tag="VGR22_12265"
     CDS             complement(2840..3580)
                     /locus_tag="VGR22_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2129383.1"
                     /translation="MPRVWTSSSPTPSRLRQRLRQQRAAGQSRPGHADPPALVPVILP
                     DAELGPALERRLLPTVTRANAGCREARDALHLAFRPKLQRFVRRITVPRAGQDTVGLW
                     DIDDVEQEAWLVFDELIRQWTPDRPFGRYVLATFPWRLRDAVYRGIARAGVPPRMTTV
                     PIGEREWLQDGSAAADEAHVLLDALAARLPALQGTILRRHVGCGETLTEIARDLDVSR
                     STVTRQWRALRDDLASGFAATPQERHMA"
     gene            complement(3957..4238)
                     /locus_tag="VGR22_12270"
     CDS             complement(3957..4238)
                     /locus_tag="VGR22_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129384.1"
                     /translation="MSSAQQPQSGSGQPRVIRMSPPAHRWECGCQQPPVLLATWTDSG
                     QVNIKVRDRYWHVSGCAVIQTTCPRCATEHQLDVKRLQELASASPSQKS"
     gene            complement(4411..5028)
                     /locus_tag="VGR22_12275"
     CDS             complement(4411..5028)
                     /locus_tag="VGR22_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129385.1"
                     /translation="MARALSSQVVQGRGSELSAEMLVRWSGMMAIVAGLITIIVTFVH
                     RSDDVRVDHAMWVPVHIPYFIGLMLLQLALVGVFAAQLRMAGRLGLVGFLLAFLGTAM
                     MLMAGREHLFSPDFGDPDATRGLWQQILASVVFSAGFITLGIGIRRAGVLPATAGILL
                     AVVGPIVAFSPPIGVLAVLIVGSVLFGAGLIWIGYALWNGRTAVR"
     gene            5295..6590
                     /locus_tag="VGR22_12280"
     CDS             5295..6590
                     /locus_tag="VGR22_12280"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2129386.1"
                     /translation="MSTIATLTSDNQLREAVAVHHAVHGDPEPIWGELLYRHPRWSAT
                     GAHRAVIDDDRIVALTSVAMWEHRFGDAILPAGEIGLVGTLSDRRNQGYSRALMESWL
                     ETMRAERIPLSFLIGIPNYYERWDYHYAAPDHANHFLSIGRDALARCAVSGASRRPVD
                     AARDIPAILDLIAAEMRHTAGSPALDATLLRYFVDRAAAHGVDWHVVEGDDGDVSGVI
                     RTKRWTGGTGPQAAGAIALVATRDDHARSAIAAVMLDHLAQGERDELPLAIAPHGPFA
                     RWLYQRGACRRSDRSIYPGGYAAMYRVNDLPTVLEAVHTTWDDREITRHLAGTAVTLR
                     TGSDETQVATLEVTQEEIEIHPGPGGVETDAPPAVSVPWVTGWRSAADWLDGTPFPPL
                     PGRTFDRGEPETLPPDARNLLSMLFPRRHPYVGDTIQGA"
     gene            6879..8531
                     /locus_tag="VGR22_12285"
     CDS             6879..8531
                     /locus_tag="VGR22_12285"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEV2129387.1"
                     /translation="MADVLITGARVIDGTGNPWIYADVVLEGERIADVAPAGTVDRAS
                     VGTVIDADGMICCPGFIDIQSHSVVPFLTDRRSVSKVTQGVTTEILGEGWTPAPFGGE
                     IDAPFSISLVHRVEEDQYEYWDKLGRTWTRFGHWLADLESRGVSVNVGSFLGGGTVRK
                     YGMGERLGEPDEHALDLMREATRESMEDGAFGIATALIYPPGSFAGTDELVELMKVVA
                     KHNGVHITHIRSEGDEIYEGLAEAIEIAERSGVSTEIYHLKAAGRDNWPKMTTVIERI
                     NEARARGIDIGADMYPYDGSGTGLAACLPPWASAEGKRYENLKDPVMRAKIIEEMRNP
                     SGSWENLGARSGPDHVLLAEFRKPENKKFQGRFLSDVADELGMDWHEAICHLLDSEGN
                     DIFTMYLMISEENLEQQFKQEWIKFSTDAGGIDPERDAERGLVHPRAYGTYPRILGRY
                     VREKGYMSWEDAIRKASSAVADRLGLRDRGLLRAGMYADVVVFDPETAIDKATYTDPH
                     HLSVGIRDVFVNGTAVLRNGEHTGALPGKRVNGPGYTPEVGA"
     gene            complement(8940..9923)
                     /locus_tag="VGR22_12290"
     CDS             complement(8940..9923)
                     /locus_tag="VGR22_12290"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEV2129388.1"
                     /translation="MRPVLAALLMLLVLTGLFAPATARQEADDGVEQARGSVVTVYTY
                     HPGGPFSRGQTGRAPSGAGSGWVYDEGLVVTNAHVVDGAEEITVVTTAGELIPAKVVG
                     SDWYQDVAVLRLNPDDPDDLPPSLPIGDSREVEAGEPVVAIGTPHGQFADTVSEGEVL
                     AVDQRINTGQGYSMLNLIRHSALLAPGNSGGPLVNVAGDVIGMNVASRRSSDDDMQQP
                     PTGFAIASDAFVSLVEEIVETGTVARPWLGVTTRLVDGETVVHEVERGSPAAKAGVEE
                     GDRIVAVDGQSVTWAVPFIDLLYQHDQGDEIVLTVERDGAELEVPVGLDRR"
     gene            complement(9998..11443)
                     /locus_tag="VGR22_12295"
     CDS             complement(9998..11443)
                     /locus_tag="VGR22_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_223146965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CUAEP/CCAEP-tail radical SAM protein"
                     /protein_id="HEV2129389.1"
                     /translation="MALSATTTAIASDTSSTLRAPGAVLLVSCYELGHQPLALATAAA
                     SLRAEGFDPTVRDLAVEELTDDDLARARLVSVSVPMHTAMRIGQGVLHRLRVAHPETP
                     VVFYGLYAVLNADHLFATGASAVIGGEFDAPLRQIAAALASGGDLVTAAIPGVMTPDN
                     RSPEPAPRSAFPTPDRAGLPVLRSYAGLEREGTIVPAGYVEATRGCHHTCTHCPITPV
                     YGGRLVVIPRDRVLGDIAQQVKMGAQHITFGDPDFFNGPAHSLRILREMRERWPGLTF
                     DATIKIEHLIEHRQRLPELAELGCQFIVSAVESLNEEALRRMRKGHDAAMVAYAIALL
                     DGIGVPMRPSLLPLSPWETLESYIDLLHFMAEHGMVRNIDPVHWSIRLLIPPGSAVLD
                     DPDTAVWIGPLDPEALTYEWTHPDPRVEALQREVTRLAETGDACQQDPAETFEAIWHA
                     AHRVAGVEAPELPVTTGHRPRLPRLTESWFC"
     gene            complement(11633..11998)
                     /locus_tag="VGR22_12300"
     CDS             complement(11633..11998)
                     /locus_tag="VGR22_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129390.1"
                     /translation="MTSLDRPGSMPSGELEAAFADQLERGAVDIVQDDDTGDLEVHAD
                     GWTLALQGTPPAVAFLAIDDEPAEEPELDSALDEVVSDEDLGAFRRLDADFNGALRAA
                     LEASGDPLSIALASRLALP"
     gene            complement(11995..12894)
                     /locus_tag="VGR22_12305"
     CDS             complement(11995..12894)
                     /locus_tag="VGR22_12305"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEV2129391.1"
                     /translation="MTLRIVAVPYRYDERNTGLGLGPQALLDDGLLGELSDAGVDVAE
                     PVFAELPDEERTDESIAVNIGRLGAHVGEQVATACGAGNRVLVLAGDDTASVGVISGL
                     QRASGAGHRIGLVWLDAHGDFNTPETSVSNLLAGMPVAILAGLAGPLWRDAAMLAATI
                     PTNRIVIAGVRELDSPEEQLMRSTDVTVLRHAEALDGTSFAAAVTRLAERVDEIYLHV
                     DLDVLDPSLVPSSSTPSPDGMTIRQLDDLLGTVYATGRVGVMGVAGLNPGGGKLGDLS
                     VASMQKLLVAAVPKWTREGGEGT"
     gene            13075..13572
                     /locus_tag="VGR22_12310"
     CDS             13075..13572
                     /locus_tag="VGR22_12310"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEV2129392.1"
                     /translation="MTSRTTPFDPDRETLYDLLGVDKRATQKDITRAYRQRMKEHHPD
                     RLPPSERERTEIFAKHINHAYATLKDPMKRKQYDDSIRAQEVQDQIMNRYVGGLGGPG
                     LGGQDLHGQRFRREMTDFERAEVRVADRSATLSLLRAVVFLTVVVIALMLGYALVASA
                     VGLLF"
     gene            complement(13475..14635)
                     /locus_tag="VGR22_12315"
     CDS             complement(13475..14635)
                     /locus_tag="VGR22_12315"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEV2129393.1"
                     /translation="MTDNDTVNRPDWTAAARRLLGDDLIVRSDADRTVAIGDTTARVR
                     LWPPDATIEHVRTVRALHDRVPDRAGVPAPVGNVDVAVLVGGRVFDACTVLEGLPLNR
                     HGMFTVEGYGAVAIPLHETADHGDILAQAARLLGMVHQASRDHIDQERTGAFSARDLH
                     TATRLSWQEARKSLGRQAEALVEVRRWLRCGNRVIPIADDRLRAAGDDATGTPVLIHG
                     DIWPARLMVDEPSRPRTLHGIDGWTMAAAGSPVIDVAALCARITGWSAANVEAVLGAY
                     SEHADLSPGARRLVPVVAAVDLLNQLGRLLEIAFLDDWVADDPAQPFVRGGIAVLLRS
                     LEHLTDVLAPPEPGGRGPGGPRRGGPPRTPPGRTRASGRSPRPSGRRPREAG"
     gene            15087..15791
                     /locus_tag="VGR22_12320"
     CDS             15087..15791
                     /locus_tag="VGR22_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2129394.1"
                     /translation="MPDTRILLVEDEPSISGFVRRGLIFEGFDVSVVTDGHEALEVLR
                     ENPPDLLILDLMLPGVDGLELTRRVRAAEEQGDARLPILMLTARDSVTDRVAGLEAGG
                     DDYLVKPFDFDELVARVRALLRRSQATQQETAGEELTFADLTVDLDSRTVRRNCREIE
                     LTPREFDLLVYFLRNPNRVLTRTQLMERVWGEDFYGESNVLEVVVGNLRRALEAEGED
                     RLIQTVRGVGYVLRQR"
     gene            15824..17437
                     /locus_tag="VGR22_12325"
     CDS             15824..17437
                     /locus_tag="VGR22_12325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2129395.1"
                     /translation="MERARSDTATSRRLPRIDFFRSIRFRLTAWYTLALIIIIALLGL
                     LLSTFMARALEDEVDQRLWNASEFVGDLLGLSEDTTRRYVVQGSNDWVQQIQNNTNDD
                     LDSMLSSGLFVIAIDLVNYDINEEGNAPEGLLNSIHLTGDTPDGALQTGEIDEQQIRV
                     LVRHVYVQSADGDELDAPPVGIVLIGESLQRQEKILQLFNQLLRAVSAGGVLLATWGG
                     WLLAGRALAPVNRITSTADEIARQGDAQESLSTRLEVNDTGDELARLAITFNAMLDRI
                     ERAFRGQRRFVADASHELRTPLTAIRGNVDVLERRARSGNDIDPEMLKESLGDMKRES
                     ARMARLIDDLLLLARTDAEGLGHMIHPEPVSLEVVAREALRTAEAVATGQRLHLTIRQ
                     PLLIYGDGDRLVQVMIILLDNAIRHTPPRGTIDLVIDAAEPDDGQQTSARIQVVDSGE
                     GIPAEHLSHLFERFYRIEGSRSRLAGGSGLGLAIALAIVRGHNGWIDVESEEGKGTHV
                     TVWLPILESPVAKQEGIPVLPHFQESPHA"
     gene            complement(17504..18901)
                     /locus_tag="VGR22_12330"
     CDS             complement(17504..18901)
                     /locus_tag="VGR22_12330"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEV2129396.1"
                     /translation="MAQDTEMRTVRDSMGEMQVPKDAMYGATTARAVENFPISNLRFS
                     RSFLRALGLTKHACASVNVDLGMLDSKLAEAIGQAATEVAEGKWDAEFPIDIFQTGSG
                     TSTNMNANEVIATRAAQLDYGDVTPHPNDHVNMGQSSNDVIPTAIHVAAYMKIAEKLL
                     PAMEHLQQTFERRADELHDVVKTGRTHLMDATPVRMGDEIGGWAAQVELARERIEASL
                     PRLAELAQGGTATGSGINAHVEFGGRVAKKLEEFTGHPFRETRNHFAAQAAQDTAVEV
                     SGQLKAYAAGLMKIANDLRWMNSGPQAGLAEIVLPALQPGSSIMPGKINPVIAEATMM
                     VCAQVMGNDTAITVGAQMGNFELNVMLPVIAHNQLESIELLASVSNVLADKAVAGFTV
                     NREWIAELVEKNPVMVTALNPVIGYERGAKIAKQAYADGRRVIDVAVEMTELAEEDLA
                     RILDPASLAGPGIKE"
     gene            19070..19987
                     /locus_tag="VGR22_12335"
     CDS             19070..19987
                     /locus_tag="VGR22_12335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEV2129397.1"
                     /translation="MEVSIMIEGQQGLSWPRWQRLVHAAEDLGFDGIYRSDHFVEPQG
                     NYQDALELWVSFAWLAANTSRIQFSPLVSPVSFRDPVITAWQASGVDALAGGRLRLGL
                     GAGWNAREHEAFGYELLDVPQRFARFEEGLQVICRLLRENGPVTFEGDYYQLRNAQIA
                     PRSPRENGPPILIGGNGPKRTLPLVAKYADEWNATSMPMDDYRERTQRLNELLEEQGR
                     KPEDVKRTVMRRGVIGRDDTEVNQKLDGADKEAMLARGLVIGTPSEVVEILGTFAEAG
                     IDGVMLQWLDMDDISGLELLAAEVLPQVR"
BASE COUNT         3562 a         6923 c         6551 g         3465 t
ORIGIN      
        1 gggtagtaga ccgtttcgac ctcatcctgc gcctgcagcc attcggcaac cttggctgcg
       61 ttcgcgctgt ggcggtccat gcgcacgccc agcgtcttga tgccgcgcag cgtcaaccag
      121 caatccatcg gtcccggcac cgcaccggcc gcgttctgat ggaaagcgag gatctgctgc
      181 acctcggggt cgttgccaac cgccacgccg ccgacaacgt cggagtgccc gccgaggtac
      241 ttggtcgtgc tgtggagcac gaagtccgca ccgaggctga gcggccgctg taggtagggc
      301 gaggcgaagg tgttgtcaac caccagccag gtgtctgggc ccttcagcgc ctctgcccag
      361 ccggcaatgt cgacgatgcg caggtacggg ttggtcgggc tctcgaccca gaccatcttc
      421 gtgttctcct gcacggcgtc gcgcgcctcg tcgaggttgg aaaggtcggc cagcgacatc
      481 tcgatgccgt agttgcggag taccttgtcg aagagtcgga aggtaccacc gtagctgtcg
      541 ctggaaagca acacatgatc ccctgggcgc agcaagtagg caagattgtg cgtcgcggcg
      601 agcccgctgg cgtaggccat gccccacgcg ccgccctcga gcgaggcgag cgcatcctgc
      661 aatgcagtgc gagtcgggtt gccggtgcgg ctgtactcgt accccttgaa gttgccaatc
      721 ccttcctgtg cgtaggtgct ggtctggtag atcggcgtga tcaccgcacc cgtagcgggg
      781 tccgggtcct gtccggcgtg gatcgcgcgc gtctcgaacc tgctgtcgtt ccaatcctgc
      841 gtcatgtcct gtctctccgt gaacagggcc atcatggaga tcgcccgtac aggtcgactc
      901 catgtcgacc ccggttattg ccagctcgcc aacggccggg aaaggcgtga agctttccca
      961 ttcgggcgag ctccatgccc accctggttc cgccacgtct ggttagcgct gcttcgcgac
     1021 gtggttgagg atgtcggcac gtgtaacgat gccgatcagc ttgccctcgt gctgcaccag
     1081 cactgccggc gccttctcgg ccagcatcgg gatcactcgc tcgatatcct cgtgcgagtc
     1141 gatcagctgg aacgccggtt ccatgatctc gcgtaccggc ttgttcagca cctccgggtc
     1201 cttgaagatt tcgtcgagca gcttgcgctc ctgcagcgag ccgatcacgc tgtggacctc
     1261 gatttcctcg ccaggctcgg gccccttgca ctccttaccc gcctgggcgc cgctgccgcg
     1321 cacgaccggc agctggctga cgccgtactc gttgagcagc gcgatcgcct gcgacaccgt
     1381 ctggtcgcag gcgacggcga tgacgtccgg cacattgcgc tcgcgggtgt cgatcagact
     1441 gcccatgtcg gacggacccc ggaagcgtgc gaggaagccg tgctcgcgca tccagtcgtc
     1501 gttgtagatc ttggagaggt agcctcgccc gctgtccggc aggaccacga ccaccatcgc
     1561 atccggatca tcgatgcgcc gcgcggactc gaccgcggca tgcaccgcca tgccaccgga
     1621 tccgccgatg aggatgccct cctcgcgggt cacgcgccgc gccatcgtga acgcctgggt
     1681 gtcgttgacc tgcacccact cgtcgacgat gttccgatcg aaggtgcccg gccagaagtc
     1741 ctcgcccacg ccctcgatga ggtactggtg gatattatcg gtcgtgtaga tcgagccctc
     1801 cgggtccgcg ccgacaacga cgactccggc gctctgctcc ttgaggtagc gcccgatgcc
     1861 ggtcaccgtg ccgcccgtac cgacgccagc gacgaggtgg gtgatcttcc cctcggtctg
     1921 gttccagatc tccggtccag tggcgtcata gtgcgcctgg ggattcatct gattgaaata
     1981 ctggttcggc gagaacgagt tcgggatctc ccgcgtcagc cggtcggcga ccgagtagta
     2041 gctcttcggg tcgtcacgct cgactgaggt cggggtggtg actacctcgg caccgtaggc
     2101 gcgcaacagc tgcaccttct cgttggagac cttgtctggc atgacgaaga tgcatcggta
     2161 gccgcgcacg gccgccgcca tcgccagccc gacaccggtg ttgccggagg tcggctcgat
     2221 gatcgtgccg gccggccgga gccagccgcg atcctcggcc tcctcgatca tgcggatacc
     2281 gatgcggtcc ttgaccgacc caccgggatt cagcatctcc agcttggcat agaccggcgt
     2341 gcggatgtct ctggtcaccg agttgaggcg taccagcggc gtgttgccca gcgcctcgat
     2401 cacgttctcg tagaccccgc catcaggacg cttcacaacg ccgttggcgc gctcgcgggc
     2461 gcggtcggcg gtgaggtgcc gtgcgcttcg aagactggtc atggccagcc acctttctgt
     2521 ctcgttctgg cttgcgtctg cagatcgtgg gtagcgcggc gggcgtgtcg ggcgtggtgc
     2581 acggcgccaa gtgggtcgat gaagacctgc accgcacggc gctggcgggt ctaccgccgg
     2641 acacggccgc cgctgataca ggcgcaacag ggggagatgc cggtgagcgt ccagcggtcg
     2701 agcaggtcgg ctgccgtgca tccgtcacta ctggggatgg tcgagctacg ggtcatgtcg
     2761 gcgatcgtca tcgcgatccc ttccgaaagc acggcacccc atcgtgccag ccccgactgc
     2821 gggtcaatct cgccctatct tacgccatgt gccgctcttg cggtgtggcg gcgaacccgc
     2881 tggcgaggtc gtcgcgcagc gcccgccact gtcgcgtgac ggtgctgcgg ctgacatcga
     2941 gatcgcgggc gatctcggtc aaggtctcac cgcaaccgac atgcctccgc aggatcgtcc
     3001 cctggagggc cgggagccgg gcagcgagcg cgtcaagcag cacatgggcc tcatcagcgg
     3061 cggcgctgcc gtcctgcagc cattcgcgct cgccgatcgg cacggtggtc atgcggggcg
     3121 gcacgccggc ccgggcaatg cctcggtaga cagcatcgcg cagccgccac ggaaaggtcg
     3181 ccagcacgta gcgcccgaag ggacggtccg gcgtccactg ccggatcagc tcatcgaaga
     3241 ccagccacgc ctcctgctcg acatcgtcga tgtcccagag cccgacggtg tcctgtccgg
     3301 ctcgcggcac ggtgatgcgt cgcacgaacc gctgcagctt gggccggaag gcaaggtgaa
     3361 gtgcatcgcg tgcctcacgg caaccggcgt tggcgcgagt cacggtcggc agcaggcgac
     3421 gctcgagcgc cgggccgagc tcggcgtccg gcaggatcac cggtaccagc gccggcggat
     3481 cggcatggcc cggccgagac tgtccggcgg cgcgttgctg gcgcaaccgc tgccggagcc
     3541 gggaaggagt tggggacgaa gaagtccaca cgcgtggcat gaagggagag cctccgaaga
     3601 aacgtcgaaa cgggatcaag gctcttcgga gctcgtgggg atgggttgca gagcgaggtg
     3661 atgtggaagc tggcgatcgg caatcgatgt acgcacgggc caacaatccg ttggggccga
     3721 cctccggagg tcggccccgc cgcggggcgg caacgtgtgc ctatacaggc tcgtatcaat
     3781 caagcgacct tgtcatttcg agccggcgag gcgggtccgc gccgaagccg tatcgagaaa
     3841 tctcttgcgc gataggcgcc cagttatcga atggcccgac tcttggcggg agcgatctct
     3901 ccactccacg cactcatgcg catctcggtc gaaatgacag cctttccctg gaatacttac
     3961 gatttctgtg acgggctcgc gctcgccaac tcctgcaggc gcttcacgtc aagctggtgc
     4021 tcggtcgcac agcgtgggca ggtggtctgg atcaccgcgc aaccggagac gtgccagtag
     4081 cgatcgcgga ccttgatgtt gacctgcccg ctgtcggtcc aggtcgccag cagcactggt
     4141 ggctgttggc agccacattc ccagcggtgc gccggcgggg acatgcggat cacgcggggc
     4201 tggcctgagc cggattgcgg ttgctgagcg gatgacatga gcgtggtccc tccccaaacg
     4261 atggtcgact ggcatggcgt gtcaaggaca tgagccggtg tgccgtgagt agaacatatg
     4321 ttctaatttt cgagtctaga gggcgccggg tcatctgtca agatgtcgcg tggagcgaac
     4381 ggtcaggcca atcaacccgg ccattcggcc tcatcgcacg gcggtgcggc cgttccagag
     4441 cgcgtaccca atccagatca gccccgcgcc gaagagcacg ctgccgacga tcagcaccgc
     4501 cagcaccccg atcggcggcg agaacgcgac gatcggtccg acaaccgcga gcaaaatccc
     4561 agccgtcgcc ggcagcacgc ccgcccgccg gatcccgatg cccagcgtga tgaaccctgc
     4621 gctgaagacg acgctggcga ggatctgctg ccacaggcca cgcgttgcgt cgggatcgcc
     4681 aaaatccggc gagaagagat gctcccgccc cgccatcagc atcatcgccg taccgaggaa
     4741 cgcgagcagg aacccgacca gccccaggcg ccccgccatc cggagctgtg cggcgaagac
     4801 gcccaccagt gccagctgca acagcataag cccgatgaag tacgggatgt gcaccggcac
     4861 ccacattgcg tggtcaaccc tgacatcatc gctccggtgc acgaacgtca cgatgatcgt
     4921 gatcagcccg gcgacgatcg ccatcatgcc actccagcgg accagcatct ctgccgacag
     4981 ctcggatccc cttccctgca cgacctgtga actcagcgcc ctggccatcc cgacctcctt
     5041 cactatggag atgctgcatg acgaacatga aagcgcacac tgcgccctga cgaaccgggc
     5101 cgcaaacccg tgatcggtga tgcctgcgtc gactccatgg aacgcggacg gtggtccgga
     5161 gaatcaccta gttggtagtg tctcgttcac ctgtcagtgt agcgatctcg ctacattgcg
     5221 aaaagctcct aacacgcagg accgaatggc agcacaatga agcagcatcg cagaagccga
     5281 ggaggtgcag ccgagtgtcg acaatcgcca ccctcaccag cgacaaccag ctccgcgaag
     5341 cggttgccgt ccatcacgct gtccatggcg atcccgagcc catttggggg gagcttctct
     5401 atcgccaccc gcgctggagc gcaaccggcg cccatcgggc agtcatcgac gacgaccgca
     5461 tcgtcgcgct gacctccgtc gccatgtggg agcatcgctt cggcgacgcc atcctgccgg
     5521 ccggggagat cggcctcgtc ggcaccctgt cggatcgccg gaaccagggg tacagccgcg
     5581 cgctgatgga gagctggctg gaaaccatgc gcgcggaacg catccccctc tccttcctga
     5641 tcggcatccc gaactactac gagcgctggg actaccacta cgcggcaccg gaccacgcca
     5701 accacttcct ctcgatcggc cgcgacgccc ttgcacgatg cgccgtctcg ggtgcatccc
     5761 gtcggccggt cgacgcggcg cgcgacattc ccgccatcct cgacctgatc gccgccgaga
     5821 tgcgtcacac ggccggctcg ccggcgctcg atgcaacgct gctgcgctac ttcgttgatc
     5881 gcgccgccgc gcacggcgtc gactggcatg tagtggaggg tgacgacggt gacgtctccg
     5941 gcgtgattcg cacgaagcgc tggacgggcg gcaccggacc ccaggcggct ggcgcgatcg
     6001 ccctcgtcgc cacccgcgat gaccatgccc gaagcgcgat cgcggccgtc atgctggacc
     6061 accttgcgca gggcgagcgg gatgagttgc cgctcgcgat tgcgccgcac ggtcccttcg
     6121 cccgctggct ctaccagcgg ggcgcctgcc ggcgctccga ccgctcgatc tacccgggcg
     6181 gctacgccgc gatgtaccgc gtcaacgatt tgccaaccgt gctggaggcg gtgcacacga
     6241 cctgggatga ccgggagatc accaggcacc tagcaggtac ggcggtcacg ctgcgcaccg
     6301 gatccgacga gacccaggtc gcgacgctcg aagtcactca ggaggaaatc gagatccatc
     6361 ccggtcccgg tggggtcgaa accgacgcac cgcccgccgt gtccgtgccc tgggtgaccg
     6421 gctggcgctc ggcagcggac tggctcgacg gcacgccgtt cccaccactc cccggccgaa
     6481 ccttcgaccg cggtgagccg gaaaccctcc cgcccgacgc ccgtaacctt ctcagcatgc
     6541 tcttcccccg tcgccacccc tacgtcggcg acaccatcca gggcgcgtga tcggctccca
     6601 tcgtaaccag tctgtagacc cgcgcatctg agcgcgggct tcggaatgga cttagcactc
     6661 accagcccag agcgaccgat gttcccgtca accagtactc cccggctgca agtgaccggc
     6721 acagaacccg gcaggacaca ctcggttcgc ggcaccccgc ctccggcgcg ccgctcttga
     6781 cgacgtcctg aatcccggtg accctgtcgg catcgaccag acggcagccg cgcctgccgt
     6841 catttccatg gccgccacca gacgaaagcg agaggtttat ggccgacgtc ctcatcaccg
     6901 gcgcccgcgt catcgacgga accggtaacc cctggatcta cgccgatgtc gttctcgagg
     6961 gcgagcgcat cgccgacgtc gctccggcgg gcaccgtcga cagggcgagc gtcggtaccg
     7021 tcatcgatgc tgacgggatg atctgctgcc ccggattcat cgacatccag tcccactccg
     7081 tcgtcccgtt cctcaccgac cgccgctctg tctccaaggt cacccagggc gtcaccaccg
     7141 aaatcctcgg cgaggggtgg accccggccc ctttcggcgg cgagatcgat gcccccttct
     7201 cgatctcgct cgtccaccgc gtcgaggagg accagtacga gtactgggac aagctcggcc
     7261 gcacgtggac ccgtttcggc cactggctcg ccgacctcga atcgcgcggc gtctcggtca
     7321 acgtcggctc cttcctcggc ggcggcacgg ttcgcaagta cggcatgggc gagcggttgg
     7381 gcgagcccga cgagcacgcc ctcgacctca tgcgcgaggc cacccgcgag tcgatggagg
     7441 acggcgcctt cggtatcgcc accgcgctca tctacccgcc gggctccttt gccggcaccg
     7501 acgagctggt cgagctgatg aaggtggtgg ccaagcacaa cggcgtccac atcacgcaca
     7561 tccgctctga gggtgacgag atctacgagg gactggccga agcgatcgag atcgccgagc
     7621 ggtccggcgt ctccaccgag atctaccacc tcaaggcagc gggtcgtgac aattggccga
     7681 agatgacgac cgtgatcgag cgcatcaacg aggcacgcgc ccgcggcatc gacatcggcg
     7741 ccgacatgta cccctacgac ggctccggca ctggcctcgc cgcctgcctg ccgccatggg
     7801 cgagcgccga gggcaagcgc tacgagaacc tgaaggatcc ggtcatgcgg gcgaagatca
     7861 tcgaggagat gcgcaacccc tccggcagct gggagaatct cggcgcccgg tccggaccgg
     7921 accacgtgct gctggccgag ttccgcaagc ccgagaacaa gaagttccag ggccgctttc
     7981 tctccgatgt cgcggacgag ctcggcatgg actggcacga ggcgatctgc cacctgctcg
     8041 acagcgaggg caacgacatc ttcacgatgt acctcatgat cagcgaggag aatctcgagc
     8101 agcagttcaa acaggagtgg atcaagttca gcaccgatgc tggcggcatt gacccggaga
     8161 gagacgctga gcgtggcctc gtccacccgc gcgcctacgg cacctatccg cgcatcctcg
     8221 gccgctacgt ccgcgagaag ggctacatga gctgggagga tgcgatccgg aaggcgagct
     8281 ctgccgtcgc cgaccgcctc gggctgcgcg accgcggcct gctgcgcgcc gggatgtacg
     8341 ccgacgtcgt cgtcttcgac ccggagacgg caatcgacaa ggcgacgtac accgacccgc
     8401 accacctatc ggtcggcatc cgggacgtct tcgtgaacgg tacggcagtc ctgcgcaacg
     8461 gcgagcacac cggggcgctg cccggcaagc gcgtcaacgg tccgggctac acgcccgagg
     8521 tcggagcgtg acgccctgca gggcacaacg agcaacagcg gtgtccgatg tggacaccgc
     8581 cgccacccag ggatgtgggg aggagagaag aagaacgacg ctgaggttca tcagcgttga
     8641 tatcgccctt caattgatga agtatggagc cggcatctga acgatcgctg acccgagcat
     8701 gaaaacaggc tgaagaacgt ggggatgccg gggatgggca gaagatatcg cctcgaaaac
     8761 gcatcacggg cggctccaga tctggaaccg cccgtgaaga ggggtgggga ggagagaaga
     8821 aagcgaagac ttcgcttcgt ccttccaatc atgagtttat ggtggcggcc tgaacggcga
     8881 ctgaatgatt cctgaagaat atctgaaggt ttccgtacat cctgctcgta cacgttggat
     8941 catcgcctgt cgaggccaac cggcacctcg agctctgcac catcgcgctc taccgtgagc
     9001 acgatctcgt cgccttggtc gtgttggtag agtagatcga tgaacggtac ggcccacgtg
     9061 accgactggc catcgaccgc gacaattcga tccccctcct cgactcccgc cttcgcggcc
     9121 ggtgagccgc gttccacctc gtgtacgact gtctccccat cgacgagtcg cgtcgtcact
     9181 cccagccatg gccgcgcgac cgttcccgtt tccacgatct cctcgactag cgacacgaaa
     9241 gcgtcgctcg cgatcgcgaa cccggtgggt ggctgctgca tgtcgtcatc gctcgaacgc
     9301 cggctggcga cgttcatccc gatcacatcg cccgcgacat tgaccagcgg cccacccgag
     9361 tttcccgggg cgagcagcgc cgagtgccgg atcaggttga gcatcgaata tccctggccg
     9421 gtattgatgc gctggtcgac cgccagcacc tctccttcgc tgaccgtgtc ggcgaattgt
     9481 ccgtgcggcg taccgatcgc cacaaccggc tcgccggcct ccacctccct gctgtcaccg
     9541 atcgggagcg acggcggcag atcgtccggg tcgtcagggt tgagccggag tacggcaacg
     9601 tcctggtacc aatcgctccc aacgaccttg gcagggatca actcccccgc cgtcgtcacc
     9661 acggtgatct cctcagcgcc gtccaccacg tgcgcattgg tgaccaccag gccctcgtcg
     9721 tacacccagc ccgaccccgc accggacggc gcacgtccgg tctggccgcg cgaaaacggc
     9781 ccaccaggat ggtaggtata aaccgtgacg actgaaccgc gcgcctgctc gacgccatcg
     9841 tctgcttcct gccgtgcggt tgcgggagcg aacagcccgg tcagcaccag cagcatcagc
     9901 agcgcggcaa gcacaggtcg catggcgacg ctccggagga tcagtggaag gataccggga
     9961 ccggtcagcg cgacaactgc cgcgcggttg gctctgctca acagaaccag ctttcggtca
    10021 gccgcggtag tcggggccgg tggcccgtcg tcaccgggag ctcgggcgcc tcaacacccg
    10081 ccacgcggtg agctgcatgc cagatcgcct cgaatgtctc cgccgggtcc tgctggcagg
    10141 catcgcccgt ttcggcgagc cgggtgacct cccgctgcaa cgcctccacg cgcggatccg
    10201 ggtgcgtcca ctcgtaggtg agggcctcgg gatcgagtgg tccgatccat acggcggtgt
    10261 ccgggtcgtc gagcacggcg gagccaggcg ggatcagcag tcggatgctc cagtgcaccg
    10321 gatcgatgtt gcggaccatt ccgtgctccg ccatgaagtg cagcaggtcg atgtagctct
    10381 ccagcgtctc ccagggcgag aggggcagca gcgatggccg catcggcacg ccgatcccat
    10441 cgagcagcgc gatcgcgtag gcgaccatcg ccgcatcgtg ccccttgcgc atgcgccgca
    10501 gagcctcctc gttgagcgac tccacagccg agacgatgaa ctggcaaccg agctcggcca
    10561 gctccggcaa ccgctgccgg tgctcgatca ggtgctcgat cttgatcgtc gcgtcgaagg
    10621 tgagccccgg ccaacgctcg cgcatctcgc ggaggatgcg gagcgagtgt gccggcccgt
    10681 tgaagaagtc cggatcgccg aaggtgatat gctgcgcgcc cattttgacc tgctgggcta
    10741 tgtcgcccag tacccggtcg cgcgggatga ctaccagtcg gccaccgtag accggtgtga
    10801 tcgggcagtg ggtgcaggtg tggtggcagc cacgcgtggc ctccacatac ccggccggca
    10861 cgatcgtgcc ctcgcgctcg agtccggcat aggagcgcag caccggcagg ccagcgcggt
    10921 ccggtgtcgg gaatgccgat ctcggcgcgg gttccggcga ccggttgtcc ggcgtcatca
    10981 caccgggaat cgcggcagtc acgaggtcgc cgccagaggc gagcgccgcg gcgatctgac
    11041 ggaggggagc atcgaactcg ccgccgatca ccgcactcgc gccggtcgcg aaaaggtggt
    11101 cggcgttcag caccgcatag aggccgtaga acaccactgg cgtctcggga tgcgcaacgc
    11161 ggagacgatg cagcacacct tggccgatcc gcatcgcggt gtgcatcggc acgctcacag
    11221 agaccagcct cgcccgggcg aggtcgtcat cggtgagctc ctcgacggcg aggtcgcgca
    11281 cggtcggatc gaagccctcg gcccggagcg aggcggcggc ggtggcaagg gccagcggct
    11341 ggtgtcccag ctcgtagcag gaaacgagca gcacggcccc cggcgcgcgg agggtgctgc
    11401 tggtgtcgct ggctatggct gttgtcgtcg cggaaagggc catggctggc tttcggtgat
    11461 gcaggcggtg acgcgtaccc gtcatgctct caagtctacc gggcatcggc aacgcgagaa
    11521 cgatcggcat gtagccacgg cattgtggcc ggcgtagcca cgaacatccc gacgatcgga
    11581 accctcgacc gttacccgcc cttccggggg cgagccaaca ccgtatcaga tgtcacggca
    11641 gagcaaggcg cgaggcgagc gcgatcgaga gcgggtcacc agaggcttcg agcgccgcgc
    11701 gcagggcgcc gttgaagtcg gcgtcgagcc gacggaaggc gcccaggtcc tcgtcgctga
    11761 ccacttcgtc cagcgccgag tccagctcgg gctcctcggc cggttcgtca tcgatcgcga
    11821 ggaaggcgac cgccggcggc gttccctgca gggcaagcgt ccagccatcg gcgtggacct
    11881 cgaggtcgcc ggtatcgtcg tcctggacga tgtcgaccgc accccgctcc aactggtcgg
    11941 caaacgcggc ttccagctca cccgaaggca tcgatcccgg tcggtcaagg ctcgtcacgt
    12001 cccctctccg ccttctcgtg tccacttcgg taccgcggcg acgagcagct tttgcattga
    12061 ggcgaccgag aggtccccga gcttgccacc gcccggattc aaccccgcta cgcccatcac
    12121 cccgaccctg ccggttgcat agaccgttcc aagcagatcg tcgagctggc ggatcgtcat
    12181 gccgtccggc gaaggcgtcg acgagctggg cacgagcgat ggatcaagca catcgaggtc
    12241 aacatgcagg tagatctcgt cgacccgctc ggcgagacga gtcacggcgg cagcgaagct
    12301 cgtgccatcc agcgcctcgg cgtgccgcag cacagtgaca tcggtcgagc gcatcagctg
    12361 ctcctcgggg ctgtccagct cccgcacgcc ggcgatcacg atgcgattgg tggggatcgt
    12421 ggcggcaagc atcgcggcat ctcgccagag cgggccagcg agcccagcca gaatcgcgac
    12481 cggcatgcca gccagcaggt tgctcacgga ggtctcgggg gtgttgaaat ccccgtgggc
    12541 gtccagccag accagcccga tgcggtgtcc ggcgccggag gcacgctgca gcccggagat
    12601 cacgccgacg ctggcggtat cgtccccggc gaggaccagc acccgatttc cggcaccgca
    12661 ggcagtggca acctgctcgc caacatgcgc gcccaaccga ccaatattga ccgcaatcga
    12721 ctcgtcagta cgctcctcat cgggcagttc ggcgaagacc ggctcggcga catcgacgcc
    12781 agcatcggac agctcgccga gcaggccgtc atcgagcagc gcctgcggcc cgagcccgag
    12841 gccggtattg cgttcgtcat atcggtacgg caccgccacg atgcgcaagg tcatgtcgcc
    12901 tcctccggcc tgtgaacctg tcattcttcc ccgtattctg cgagaaatgc accgttcgtg
    12961 ccacccagtg tcgggaaatc ccagcacgga agtgctgggc catcggacgg taaaatgagt
    13021 agcagcaggc cggcgcaccc gcgccctatc tgtccgaaag gcagcagcgg cgtcgtgact
    13081 tcacgaacca ccccattcga tcctgaccgg gagacgctct acgaccttct tggggtcgac
    13141 aagcgtgcta cccagaagga cattacgcgc gcctatcggc agcgcatgaa ggagcaccat
    13201 ccggatcgcc tgccaccttc cgaacgggag cgcaccgaga tctttgccaa gcacatcaac
    13261 cacgcctacg cgacgctcaa ggatccgatg aagcgcaagc agtacgacga ttcgattcgt
    13321 gcgcaggaag tgcaggacca gatcatgaat cgttacgtcg gtggtctcgg cgggccggga
    13381 ctcggtggcc aggatctgca cggacagcgg tttcgacgag agatgaccga tttcgaaagg
    13441 gcagaggtgc gcgtcgccga ccgctcggcg acgctcagcc tgcttcgcgc ggtcgtcttc
    13501 ctgacggtcg tggtgatcgc cctgatgctc gggtacgccc tggtggcgtc cgcggtgggc
    13561 ctcctcttct aggcccacca ggcccccgcc cacccggttc cggtggtgcc aacacatcgg
    13621 tcaggtgctc cagcgagcgc agcagcactg cgatcccacc ccggacgaag ggctgggcgg
    13681 ggtcgtccgc cacccagtcg tcgaggaacg cgatctccag cagccggccg agttgattca
    13741 gcagatcgac cgccgcgacc accggcacca gccggcgcgc tcccggggag agatcagcat
    13801 gctcgctgta ggcgccgagc accgcctcga cattggccgc cgaccagccg gtgatgcgcg
    13861 cacagagcgc ggcgacgtcg atcaccggcg acccggcggc tgccatcgtc cagccatcga
    13921 taccgtggag tgtccgtggc cgcgaaggtt catccaccat cagccgcgca ggccagatgt
    13981 cgccgtggat gagcaccggc gtgccggtcg cgtcgtcgcc agccgcccga aggcgatcgt
    14041 cggcaatcgg gatgacacga ttgccacatc ggagccaccg gcgcacctcg acgagagcct
    14101 cagcctgccg tccaagagac tttcgcgcct cctgccagga gagtctggtg gcggtgtgta
    14161 ggtcacgcgc agaaaacgcg ccggttcgtt cctggtcgat gtgatcacgc gaggcctgat
    14221 gcaccatgcc gaggagccgg gcggcctggg cgagtatgtc accgtggtcg gcggtctcgt
    14281 ggagcggaat cgcgaccgcc ccgtaccctt ccacagtgaa catgccgtgc cggttgagcg
    14341 gcagtccctc gaggaccgtg catgcatcaa agacgcgacc accgacgagc acagcgacat
    14401 ccacattgcc caccggcgct ggcacaccag cgcgatccgg aacccgatcg tgcagggcgc
    14461 ggaccgtccg gacgtgctcg atcgtcgcgt cgggcggcca gaggcggact cgggccgtgg
    14521 tgtcgccgat ggcaacggta cgatcggcat cggaccgtac gatcaggtcg tcaccgagca
    14581 gcctccgcgc cgcggcggtc cagtcgggtc gattgacggt gtcgttgtcg gtcatcatgc
    14641 cgccagtata cgcgccggcc gaacgcgccc gctcgtctgc aggtgaggag acgtgccggt
    14701 tttttcagac attcttcagg cacggttcaa ttccggttca ggttctggcc cgattctgaa
    14761 tatcgaggga agcgacggag cgtagtgacg cggatgttac cgatcctccg gtcgctctcc
    14821 ttctctcctc cctcaccctt gatgatcggc agtcttcgtt cgcggaggct gccgattatc
    14881 gtttatgacc acggagtgga tgctgcagta ccatgaagcc aacgcgagac gtatcacggg
    14941 tacccggtat cagatggctc ctgctcatgg tccggacgct ccgccgggtg acgggtggcg
    15001 cgagactcag cctcgtcgct ccggccctct cccggaatcc cgctgatacg acgctcgccg
    15061 gccgacctga tgctgcagga ccatccatgc cggatacgcg cattctcctc gtcgaagacg
    15121 agccgtccat ctccggcttc gtgcgccgag gtctgatttt cgaagggttc gacgtctccg
    15181 tcgtcaccga tggacatgag gcgctcgagg tgctgcgcga gaacccgccg gacctcctga
    15241 ttctcgacct gatgttgccc ggcgttgacg gcttggagct cacacggcgc gtccgagccg
    15301 ccgaggaaca gggcgacgca cgcctgccga tcctcatgct caccgcgcgc gactcggtga
    15361 cggatcgcgt cgccggcctc gaagccggcg gggacgacta cctcgtcaag cccttcgatt
    15421 tcgatgaatt ggtcgcccgc gtgcgcgcgc tcctccgccg atcacaggca acccagcagg
    15481 aaaccgctgg ggaagagctc accttcgccg atctcaccgt cgatctcgac agtcgcaccg
    15541 tccggcgcaa ttgccgcgaa attgagctca ccccccgcga gttcgacctg ctcgtctact
    15601 tcctgcgcaa cccgaaccgc gtgctcacgc gcacccagct catggagcga gtctggggcg
    15661 aggacttcta cggcgagtcc aatgtcctcg aggtggtcgt cggcaatctg cgacgcgcgc
    15721 tggaggccga aggcgaggac cgcctgatcc aaacggtgcg cggtgtgggg tatgtcctga
    15781 ggcagcgatg agaatccggg ctggcagcta tcccggcacg aagatggaac gtgcacggtc
    15841 ggacaccgcg accagccgcc ggctcccccg catcgacttc ttccggtcca tccgcttccg
    15901 gctgaccgcg tggtacaccc tcgcgttgat catcatcatc gcgctgcttg gcctgctgct
    15961 gtcgacgttt atggcccgtg cgttggagga cgaggtcgac cagcggctct ggaatgccag
    16021 cgagttcgtg ggcgatctgc tcgggctgag cgaggacaca actcgacggt acgttgtaca
    16081 gggcagcaac gactgggttc aacagataca aaacaacacg aatgatgatc tcgactcgat
    16141 gctgtcttcg gggctgttcg tcatagccat cgatttggtg aactatgaca tcaacgagga
    16201 gggtaacgct cccgaaggtc tgctgaattc cattcacttg acaggcgata ctcccgatgg
    16261 tgccttgcaa acaggtgaaa tcgacgagca gcagattcgc gtcctagtac gccatgtata
    16321 cgtccagtca gctgatggcg acgagctgga tgctcccccg gttggcatcg ttctgatcgg
    16381 cgagtccctt cagcggcagg agaagatcct gcagttgttc aaccagctct tgcgcgccgt
    16441 aagcgctggc ggtgtgctgc tggcaacctg gggcggctgg cttcttgcag ggcgcgctct
    16501 cgcgccagtc aatcgcatca cctcaacggc cgacgagatc gcacggcagg gtgatgccca
    16561 ggagtcgctc tcgacccgcc ttgaggtcaa cgacaccggc gacgagctgg cccgcctcgc
    16621 gatcaccttc aacgccatgc tcgaccgcat cgagcgtgcc ttccggggcc agcgtcgctt
    16681 cgtcgcggat gcctcccacg aattgcgcac gccgctgacg gcgatccgcg gcaacgtcga
    16741 cgtgctggag cgccgtgcac gcagtggcaa cgatatcgac ccggagatgc tcaaggaatc
    16801 actcggcgac atgaagcgcg agagcgctcg catggcccgg ctgatcgacg acctgctcct
    16861 gctcgcccgc accgacgccg agggactcgg gcacatgatc catccggagc cggtctcgct
    16921 cgaggtcgtg gcgcgggagg cactccgcac ggcggaggca gtcgcgaccg gccagcgcct
    16981 ccatctcacg atccgccagc cgctgctgat ctacggcgac ggcgaccggc tggtgcaggt
    17041 gatgatcatc ctgctggaca atgccatccg gcacacgccg ccgcgcggaa cgatcgatct
    17101 ggtgatcgat gcggccgaac ccgacgacgg ccagcagacg agcgcgcgca ttcaggttgt
    17161 cgacagcggc gagggcatcc cagccgaaca cctgtcgcac ctgttcgagc gcttctaccg
    17221 cattgaggga tcgcgctcgc gcctggccgg tggcagtggt ctcggcttgg cgatcgcgct
    17281 ggcgatcgtg cggggccaca acggctggat cgacgttgag tcggaggagg gcaagggcac
    17341 gcacgtgaca gtgtggctgc cgatcctcga gtcccctgtc gcaaagcagg aaggcattcc
    17401 cgtcctcccc cacttccagg aatccccaca tgcgtgagca cccgatgaag cgcgtcagcg
    17461 ccgcagtcgt gcaggagggc ggcatcacgc cttgctgacg tctctactcc ttgataccgg
    17521 gcccggcgag gcttgccggg tcgagaatgc gtgcaaggtc ctcctctgca agctccgtca
    17581 tctccaccgc aacatcgatc acccgccggc cgtccgcgta cgcctgcttg gcgatcttcg
    17641 cgccccgctc gtacccgatg accgggttga gcgccgtcac catgaccgga ttcttctcga
    17701 ccagctcggc gatccactcc cggttgaccg tgaagccggc caccgccttg tccgccagca
    17761 cgtttgagac gctcgccagc agctcgatcg actccagctg gttgtgtgcg atcaccggca
    17821 gcatcacgtt gagctcgaag ttgcccatct gcgcgccgac cgtgatcgcg gtgtcgttgc
    17881 ccatcacctg cgcacagacc atcatcgtcg cctcggcgat caccgggttg atcttccccg
    17941 gcatgatcga cgatcccggc tgcagcgctg gcagcacgat ctccgcgagc cctgcctgcg
    18001 ggccgctgtt catccatcgc aggtcgttcg cgatcttcat cagaccggcg gcgtaggcct
    18061 taagctgccc gctcacctcg acggccgtat cctgcgcggc ctgcgcggcg aaatggttgc
    18121 gcgtctcgcg gaacggatgc ccggtgaact cctcgagctt cttcgcgacc cgaccgccga
    18181 actcgacatg cgcgttgatc ccgctcccgg tcgcggtgcc gccctgcgcc agctcggcca
    18241 gccgcggcag cgatgcctcg atccgctcgc gcgcgagctc gacctgcgcc gcccacccgc
    18301 caatctcgtc gcccatccga accggggtgg cgtccatcag gtgcgtgcgg ccggtcttga
    18361 ccacgtcgtg cagctcgtcg gcccgccgct cgaaggtctg ctgcaggtgc tccatcgctg
    18421 gcagcaactt ctccgcgatt ttcatgtagg cggcgacatg gatcgcggtc gggatcacgt
    18481 cgttgctcga ctgtcccatg ttgacgtgat cgttcgggtg cggcgtcaca tcaccgtaat
    18541 cgagctgggc cgcacgggtc gcgatcacct cgttggcgtt catgttggtc gacgtgccgg
    18601 agccggtctg gaagatgtcg atcgggaact cggcatccca cttgccctcg gcaacctccg
    18661 tcgccgcctg cccgatcgcc tctgccagct tcgagtcgag catgccgaga tcgacattga
    18721 cgctggcaca ggcgtgcttg gtcaacccca gcgcccggag aaaggagcgt gagaagcgca
    18781 ggttactgat cggaaagttc tcgaccgccc gggccgtggt cgcgccgtac atcgcgtcct
    18841 tcggcacctg catctcaccc atcgaatcgc gaacggtccg catctcggtg tcctgagcca
    18901 ttccggtgat ctccatcctg catcgttgct gaccacatct tcgctgctga cagtgtatcg
    18961 cctgcccaac ctgatacagg ggaatggtgc gaggttgccg gggcatcgta cgcttgacac
    19021 aggtggacag cccgacccaa gtggcatcat cgaggagaac tggattcaca tggaagtctc
    19081 aatcatgatc gaaggtcagc aggggctgtc atggcctcgc tggcagcgac tcgtccacgc
    19141 cgccgaggac ctcggcttcg atggcatcta tcgctccgac cacttcgtcg agccccaggg
    19201 caactatcag gacgcccttg agctatgggt ctcgttcgcg tggctcgccg ccaacacgag
    19261 ccgaatccag ttcagcccac tggtctcgcc cgtctcgttc cgcgacccgg tcatcacggc
    19321 ctggcaggcc agcggtgtcg atgccctcgc cggcgggcgg ctgcgactcg ggctcggtgc
    19381 cggctggaac gcgcgcgagc acgaggcgtt cggctacgag ttgctcgatg tccctcagcg
    19441 cttcgcccgc tttgaggaag ggctgcaggt catctgtagg ctgctgcggg agaacggccc
    19501 ggtgaccttc gagggggact attaccagct gcggaacgcc cagatcgcgc cccggtcgcc
    19561 ccgcgagaat gggccgccga tcctcattgg cgggaacggg ccgaagcgca cgctgccgct
    19621 cgtggcgaag tacgctgacg agtggaacgc gacctcgatg ccgatggacg actaccgtga
    19681 gcgaacgcaa cgcctgaacg aactgctgga ggagcagggt cgcaagccag aggacgtgaa
    19741 gcggacggtg atgcgccgcg gcgtgatcgg ccgggacgac accgaggtca accagaagct
    19801 ggatggtgcc gacaaggaag ccatgctggc gcgcggtctg gttattggca caccgagcga
    19861 ggtggtggag attctcggga cgttcgccga ggcgggcatc gacggcgtga tgctgcaatg
    19921 gctggacatg gacgacatct cgggccttga gctgctcgcc gccgaggtcc tgccacaggt
    19981 ccggtaaccc tgtgttgttg cgcgggtccg tgacggggca ccggtcggcg tttcccagag
    20041 accggcagag agtcatcctg agcggagggc cgaagtcgaa gtatcccccg gccggcgcat
    20101 atgccggccg gtccgttctt catgaacgac accaccatca tgagttgctt tcaccacagc
    20161 aacgaacaac actgtccttg gcgtgtggca agggcagggg atgcttcgac gccctcggcg
    20221 cagtaggcgc ctcgccgact agcatggcac cacttcaggt agttggagtt cgaacccctg
    20281 cgccctagtg acacaaagaa tcccggaacg acagggaaga tggggaacct cggctcaagc
    20341 ggctgggggt ttctgttggc agtgacagcc aacatgacag ccaaagtacg aggagtggtc
    20401 atttctcggc ctccagcgcg tcctcgatca atgtcagcgc gtcccgcatg gagtccccca
    20461 cgacgtggga atagacgtcc atcgtgaccg cgatatttga g
//
LOCUS       DASYWL010000352        23605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_637416, whole genome shotgun sequence.
ACCESSION   DASYWL010000352 DASYWL010000000
VERSION     DASYWL010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 23605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23605
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637416"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(747..1736)
                     /locus_tag="VGR22_12340"
     CDS             complement(747..1736)
                     /locus_tag="VGR22_12340"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HEV2129398.1"
                     /translation="MDDLFEQQTVPEYNTRAVETMTGVPADTFRAWERRHGLPSPART
                     PGNHRLYSERDIAIISWLKQMTEQGISISRAVSMARRRLRSASNAPPTESHDFSTAPP
                     SNDNGLHTLQQLLLGAFFEFDAQRANQIVEEALAIYDVETVTLDVMQPALVELGTMWE
                     RGEACMAVEHFATSFCMRKLSGLFNASEPERGSPTVIMSGVAGEMHELGLLVAALVFS
                     RCGCRVIYLGANLPGTELVEAVRNVGPNAVVLSTPTPPSVETLRDTVTCLRAIETSGT
                     EPLIGYGGAVFEDQPELRDTVDALYLGKDARRAHERLEQALRTRYQEVAYSRA"
     gene            1821..3230
                     /locus_tag="VGR22_12345"
     CDS             1821..3230
                     /locus_tag="VGR22_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129399.1"
                     /translation="MSRLHANQRRARRRELVDRSWKLSKWSRAVLVGIVVLLAASACT
                     DNTDDRQFASDPRTSEPTPAAEVPDVLTGTPRAPAHPLASPETLVDRRGAPSVVYAEI
                     AGSSRAITTDGVNRLTDGPVAAFAASPSGNQVAVVVPSATGLAYDVKIYASDGTLLQR
                     IDTVLISSPPATPADQALSEDEAVALSWSPQGGRLLLADVSGQLIDIRLDGTVEQIQT
                     RSSLSGLIQAEWSPRGDQIGVLFRDDDGYGSLALIDLSIDPASVDVIAPVGLVSGEPA
                     SVESFAWNPYGDGVLFLQGNPPDDGVRNGQVVGWNQASNTTEVIATGGQVGPTGSIRQ
                     FSIAPDGKALAYAIDIRTPEGSDFGGLYVRSLVQPQVYRVPVKPNVTVTGFWWVADGL
                     AWAGVVEAEGKAQTLVVRWIDGSRRIRTLAEIRMATMATPVAAPMPGAPAATPVPITA
                     VASFATPVAVPAEGATPVE"
     gene            complement(3319..5349)
                     /gene="dnaX"
                     /locus_tag="VGR22_12350"
     CDS             complement(3319..5349)
                     /gene="dnaX"
                     /locus_tag="VGR22_12350"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEV2129400.1"
                     /translation="MTDSLFDVEATQPSKPSTAAADPDVVPATGRQSLSLYRKYRPQS
                     FASDELFGQDHVVNTLRNAIRLDRIAHAYLFCGPRGTGKTTTARILAKAVSCLDPDPT
                     NRPCNRCEACLAINANATTDVIEIDAASNRGIDDIRELRERVKYAPTSLRTKFYIIDE
                     AHQITGAAANAFLKTLEEPPAHTRFVLATTDPEELLQTIVSRCQRFDFRRISIDAMSA
                     RLRQVAELEGIEIDEDGIATIARHATGSLRDALGLLDQVSVYSEATDDESPRVTVELV
                     RAVLGVSRNDRVEGLVRAVADRDPGAGLRIINDAVEAGEDIRQLGRQLIGYLRVLMLE
                     RASGSPDADEIAGVLAHRFDLNDLARVATIFAEIDYKARHAAIVQLPLELALVEACSA
                     PQQVSVATPTGDIPEQQSAPVPRQRSRPSLKDRVRGASMPRDAAPQRSAAPEVPQQEA
                     PETSSATPQQQTEPSRRQISEAEPAETGFEPPAEATASLPPGDLELVVERWDNIRMDI
                     KALNRRTDALLQQADPLHIEGSQLTLVAAYPFHQKRLNEDDTRRIIEEVIERRVGKKL
                     TVLCVSRDEAQSLRPRASAAAPVRDPTLQGVQSKTPRERTEAMGSETPPSPDADSEPE
                     PAAQTVRPPDPLNEEVVDAVASTPSAVSSLDIDEERIKAAMNIFDAVIIDEE"
     gene            5460..6296
                     /gene="rsmI"
                     /locus_tag="VGR22_12355"
     CDS             5460..6296
                     /gene="rsmI"
                     /locus_tag="VGR22_12355"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEV2129401.1"
                     /translation="MGTLYVVATPVGNLDDISPRAVRTLAEVSLIAAEDTRHTARLLR
                     RYEIATPVFSYHRHNERERVQRLLEALVAGDVALVSDAGTPAVSDPGAVLVCAVRKAG
                     HRVVPIPGPSSLVAAVSASGLVEGAFLFGGFLPRSGDERRRAMQRAAIVDVPLVLFES
                     PNRLIQTLDDLRTTFGDVQAVVARELTKIHEEIRAGRLSELAEHYRSAEVRGEICIVV
                     EAAPAVEAAGGTDDTAEIAARLLADGTQPSKAARALARITGMSSGDAYEVIRSVREQS
                     DR"
     gene            complement(6419..7573)
                     /locus_tag="VGR22_12360"
     CDS             complement(6419..7573)
                     /locus_tag="VGR22_12360"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV2129402.1"
                     /translation="MVTLRPIYIQVKPGDTARDRWRDFDLILFVTMLVLMGFGIMAIW
                     SAVGLPPLTTLNNGTFQALLGAVGIVILLITASIDYRFLNTLAWPLYCIGVLALIAVQ
                     TFGVELGGARNWFDFGFFLLQPAEFVRITTLIALASFVASREDELRSFINFLTSGMIV
                     ALPAGLVLIGPDLGMTMVYVAIWASALLVMRTRTLYILLVLVSLPLLGWIAWQFILED
                     YQKTRFQVSFTPEVAPLAEGFQILQARISIGAGGLWGEGLAGGTQSQLNLLSVRDSDF
                     IFAHASGMFGFVGMFGLFLALVIFLWRCLKVAEIARDQFGQVLSVGITGVLFFQAFLN
                     IGMNVGVMPVTGITLPFVSYGLSSLWSNLILVGILQSIRMHHRKLAFIRL"
     gene            7735..8292
                     /locus_tag="VGR22_12365"
     CDS             7735..8292
                     /locus_tag="VGR22_12365"
                     /inference="COORDINATES: protein motif:HMM:NF022734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HEV2129403.1"
                     /translation="MADELFSSGMPSDRDVQRLRVEALGEPGQRRFRLMAVINGETHI
                     VWMEKQQMQALGMAVEQLLEQVPHSEIPVIESNAAVEFDQNTLHQFRAGRIELGYDPT
                     SERVVINAYDLQQEDEDAQATLVLRLNLRQSAELSADAAVIVAAGRPLCPMCGQPMNP
                     EGHVCPQQNGHLPISLDDDDLMEEL"
     gene            8806..10575
                     /gene="ilvB"
                     /locus_tag="VGR22_12370"
     CDS             8806..10575
                     /gene="ilvB"
                     /locus_tag="VGR22_12370"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEV2129404.1"
                     /translation="MVATQRPPESTESTDESSVARAPEVLSGAEITCRSIEAEGVSKV
                     FGYPGGAVIPLYDALPSSSLRHVLVRFEQWAGLAADAYARATGEVGVCIATSGPGATN
                     LVTALANANLDSVPMVAITGQVGQQLIGRDGFQEVDITGVSLPVTKHNYLVQRPEDIA
                     PTIKEAFYLARSGRPGPVLVDIPKNVFVTKAEYKPPAQVGRKSYQPTTKPHVRQVRLA
                     IEEMARAKKPLIMAGHGIILSGAEAELQQFAEKTGVPVIYTLLGLGAMSEMHPLSLGM
                     MGMHGHRHVNRALEECDLLINIGARFDDRATGRLNGFCTNARIVHVDVDPAEIGKNVP
                     TDVPVVGDAKESLLMLIEEAGEAQDYSAWMSWIDGQRNRVLEAALEDRPEWPEPYSIV
                     KAIAEATDHDAIVTTDVGQHQMWAAQQLGIKHGKRWITSGGLGTMGYGLPSAIGAKIG
                     RPDLEVWTIVGDGGFQMSFNELATAVQENLDINIAIINNGYLGMVRQWQDLFHNHNYS
                     EVRISAPDFVQLAESFGVTGIRVTTDDEIMPAVERARSIKGPVVIDFIVEPEANVYPM
                     VPPGGSNSEMVHDPQWTPHEEDV"
     gene            10578..11117
                     /gene="ilvN"
                     /locus_tag="VGR22_12375"
     CDS             10578..11117
                     /gene="ilvN"
                     /locus_tag="VGR22_12375"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEV2129405.1"
                     /translation="MKHAHTLAVLVEDQPGVLNRVVSLMRRRSFNIDSITVGHSEQPG
                     MSRMTLVVRGEDVEVEQVTKQLYKLLEVQKVTDLSEFDSVTREMVMVKVAAKGQSRTE
                     ILQLVQVYNARVLDAMPSTLMIEATGDEQQIDSFLAMMRVFGIREMVRSGKIAMSRTS
                     GATRAKGEVTANGVSTAAD"
     gene            11243..12286
                     /gene="ilvC"
                     /locus_tag="VGR22_12380"
     CDS             11243..12286
                     /gene="ilvC"
                     /locus_tag="VGR22_12380"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV2129406.1"
                     /translation="MPATIYYETDATLAPLQDKTIAIIGYGSQGRAHALNLRDSGLSV
                     VVGLHEGSKSRAKAEADGVRVESAADAAKEADVIMMLVPDQTQKKVYEESVKPHLDVS
                     KTLMFAHGFNIHFKQIESPQGVDVTMIAPKAPGTRVRELYEEGMGVPALVAVHQDASG
                     NAKEQALSYARALGSLSAGVLETSFEEETETDLFGEQAVLCGGVSALIQAGFETLTEA
                     GYQPESAYFEVLHELKLIVDLIYEGGLTQMREVVSDTAEYGDYYAGPQIITEDTKQAM
                     KNLLGKIQDGTFAREWIAENEAGRENFMRMRQEHTDHEIERVGRQLRAMMPWLKSGKA
                     AREDAKAAMSASQ"
     gene            12369..13952
                     /locus_tag="VGR22_12385"
     CDS             12369..13952
                     /locus_tag="VGR22_12385"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEV2129407.1"
                     /translation="MSDRESNAGVEQSRVIVFDTTLRDGEQSPGATLTTDEKLEVADA
                     LVALNVDVIEAGFPAASPGDFEAVREIARRSKGVSIAGLARANTRDIERAAAAVHEAE
                     APRIHTFIATSDIHLKHKLRMTRQEVLERVREMVAFSKSFVSDVEFSCEDATRSDWDY
                     LVQVFQAAIEAGATTINVPDTVGYTTPNEFVGLMHHLRSNVDRIDDVIISVHCHNDLG
                     MATANTLAAVKTGARQVEVTVNGIGERAGNTALEEVVMALQTRGEQFGGVHTNVETTR
                     LVPASRLVSGLTGLQVQVNKAIVGANAFAHEAGIHQDGMLKERTTYEIMNPEDVGWEG
                     TRLVLGKHSGRAGFKNALKELGLVLDGDQVQGVYERFLELADRKKKVTGADISALVAD
                     EVAGEEGRYELVRWNAAIGTGGAATASVVLSQDGREIAGDGEGNGPVNALYAAIDNAV
                     QIGNELEYYHVEAVTPGEDAQGQVHVRIRVGQNLYSGHGLATDIVEASAKAYLSALSR
                     VDQDQPDTVLMGSSTSRWS"
     gene            13956..14711
                     /locus_tag="VGR22_12390"
     CDS             13956..14711
                     /locus_tag="VGR22_12390"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2129408.1"
                     /translation="MREPDVPDASRRDVIWRRDDTVANYLGATRQAIPLAAEQLDAAM
                     RAIGAFAVPTRSVLDVGSGGGSAAAAISQCFPVERITLVDFSMPMLRQRIVRFEGSPW
                     LVDLIEADLQDPAWQDELPADQPAYDLVVSRYAIHHLPDDRKQDLYAEIFGRTAPGGL
                     FVNIEHVSSVAPVYEDIFESLIVEGMAATSETGQDLAAATTAFHARQDRETNILAPAD
                     LQCGWLRDTGFVDVNVLMKTFELAVIVGRRPNM"
     gene            14806..16221
                     /gene="leuC"
                     /locus_tag="VGR22_12395"
     CDS             14806..16221
                     /gene="leuC"
                     /locus_tag="VGR22_12395"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF004016.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEV2129409.1"
                     /translation="MPKTLAEKLWDRHMVHSAPGEPDLLYIDLHLIHEVTSPQAFDGL
                     RLAGRAVRRPDLTVGTADHNVPTIGISLPVADPISRKQLEKLTENCREFGIELYPMGH
                     PGQGIVHVIGPELGLTQPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLPQ
                     SKPKTMAITLNGDLQNGASAKDVILAIIGKIGTGGGIGSMVEYRGSVIESLSMEGRMT
                     ICNMSIEAGAKAGMVAPDDTTYRYVEGRPHAPKGKLWEQALEDWQSLRTDEDAEFDHE
                     VGLDGSTIQPHVSWGTNPGQVAPIDSRVPDPDSYQSASDRNAAERALQYMALKPGTAI
                     RDIAVDKVFIGSCTNSRMEDLRAAAEIVAGQRLANNVQAMVVPGSMRVKAQAEAEGLD
                     RIFTDAGFEWRAAGCSMCLGMNPDQLQPGERCASTSNRNFEGRQGKGGRTHLVSPQVA
                     AATAIAGTFATPTDILAGDRA"
     gene            16218..16829
                     /gene="leuD"
                     /locus_tag="VGR22_12400"
     CDS             16218..16829
                     /gene="leuD"
                     /locus_tag="VGR22_12400"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEV2129410.1"
                     /translation="MNPIETIEGRAVPLDRSDVDTDQIIPASYLKRVERTGFGEGLFA
                     SWRENDKNFVLNRAEFEGASIMVAGPNFGTGSSREHAVWALTDYGIKAVICPQFGDIF
                     RNNATKSGLVPVQVDPDFGAELLEATAEHPFLEIKIDIRNRKVSAPAVGLEATFPLDE
                     FTQYRLLEGLDDIGLTLRNEDKISEFEQHRPAYKPRLETAAAR"
     gene            16904..17977
                     /gene="leuB"
                     /locus_tag="VGR22_12405"
     CDS             16904..17977
                     /gene="leuB"
                     /locus_tag="VGR22_12405"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEV2129411.1"
                     /translation="MQATIVTFPGDGVGPEVMGQALRVLQAVAEQNGHAFAFDERKIG
                     GIAIDAYGTPLRDEEIEAARDADAILLGAVGGPKWDNPNAAVRPEQGLLRIRKALRLF
                     ANLRPVVLQESLIGASPLRPERVKGTDLVVVRELTGGLYFGKPSKQWRERRGQVAVDT
                     LVYREHEIERVVRLAFQLAQGRSKKVTSVDKANVLSSSRLWRSVVTEVAAEYPDVQLE
                     HMLIDAMTMRLIQTPSSFDVIVTENMFGDIITDEASVLGGSIGLLPSASLGAEREGQR
                     RKGLYEPIHGSAPDIAGKGTANPAGMILSAAMMVRLSLGLEQEAVSVERAVASAIDDG
                     VRTADLGGDASTEMFGDAVVQRL"
     gene            complement(18061..18876)
                     /locus_tag="VGR22_12410"
     CDS             complement(18061..18876)
                     /locus_tag="VGR22_12410"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEV2129412.1"
                     /translation="MPRPIRTTLPRPPRRRLRLRGPAAWWPPVSARQDEAFRNIWQAL
                     RQHGQVQAVTLGSEKIQDRADTFVVCAIRLPRDKTASALKPLRAALSEFPFVRLAPAE
                     SLAITIQELGFLVDEPQRIDEISQERIEEFVHHSSTPVCDFPRFEIRVGGFNSFLDTP
                     FLDVHDDGWCFRMHHRLRDFTLLPVADDYAYLPHVPLGYYTRDETMRGFPARMARWRD
                     RTFVMFHAEALELLAIATADPFSEPEILHRFDLGHSLGPAETITQRGPSDVFV"
     gene            complement(18891..19370)
                     /gene="greA"
                     /locus_tag="VGR22_12415"
     CDS             complement(18891..19370)
                     /gene="greA"
                     /locus_tag="VGR22_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEV2129413.1"
                     /translation="MDKERIEITEEGHERLQEELRQLIEVERPKIIEAVADARSHGDL
                     RENSAYDAARQDQAMIEKRISDLEATLRNATVVARDAANRKRSTVTLGSKVAVDMEGE
                     EEVFTIVGAAEARPSAGFISNASPIGKALDGRRAGETVSVSTPGGHLDITIRSVDNS"
     gene            complement(19542..19994)
                     /locus_tag="VGR22_12420"
     CDS             complement(19542..19994)
                     /locus_tag="VGR22_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEV2129414.1"
                     /translation="MSVDDRLKELGIELPEPVSPAANYVRHQQTGNLLFISGTGPTDA
                     SPKGKVGADVSVEDAYQAAREVALQILATARQALGGDLGRITRTVKVLGMVNSAPDFG
                     EQPRVINGCSDTLVEVLGEKGRHTRSAVGFAALPNNICVEIEATFEVE"
     gene            complement(20036..21229)
                     /locus_tag="VGR22_12425"
     CDS             complement(20036..21229)
                     /locus_tag="VGR22_12425"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV2129415.1"
                     /translation="METRRQPADFLADRVKNATPSPTLAVSDKARQLKAEGIDVIDLG
                     GGDPDFITPEHVRHAAAEAMNSGETHYVASPGTPAFRKAIASKLRDDNGLAYDPNDEI
                     LVTPGGKAALFEAAMALVEPGVDVLVVEPAWVSYAPQIELAGGNVVAVPTSPEDNWVV
                     TEQALEAHVTPNSRVLLINSPNNPTGHVMDRKELKTIVTFANRHDLLVWTDEMYEKIV
                     YDDHEHVSIATFDGMKERTLTFNGLSKAYAMTGWRLGYVAGPRPLISKLATVHGHAVT
                     CATSFAQAGGVAALEGPQDVIGEMVQAWDRRRKLITEGVNGINGLSWMPAEGAFYAFV
                     DGRETGLSSTDLAAKMLEEAHVAVVPGIAFGEAGEGHVRLSFATSDDALSSTIERLTR
                     LFGTR"
     gene            21644..21940
                     /gene="groES"
                     /locus_tag="VGR22_12430"
     CDS             21644..21940
                     /gene="groES"
                     /locus_tag="VGR22_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEV2129416.1"
                     /translation="MASNSLKPLGDRLVIKPASREEQTASGIVLPDTAKEKPQRGTVI
                     AAGDGRRDEDGDRIPMEVKVGDEVIFAKYAGTEFKLDEEDVLILSEKDVLAVIG"
     gene            21996..>23605
                     /gene="groL"
                     /locus_tag="VGR22_12435"
     CDS             21996..>23605
                     /gene="groL"
                     /locus_tag="VGR22_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779552.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEV2129417.1"
                     /translation="MAKVLEFNEQARHSLKEGVDTLANAVKTTLGPKGRNVALDKKFG
                     APTVTHDGVTVAKEIELEDHFANMGAQLLKEAATKTNDAAGDGTTTATVLAQAIVHEG
                     MRNVAAGANAMMLKQGIDLASDAIVARIREVATEVKDKEEISQVAAISAADKEIGDLI
                     AEVMDKVGKDGVITVEESRGLAFETEYTEGMQIDRGYISPYFVTNTERMEASFEDPFI
                     LITDKKISSIQDILPTVEMVARQGKGLVIIAEDVDGEALATLVVNKLQGRFQSLAIKA
                     PGFGDRRKEMLRDIAALTGARVISEEVGRKLDSVTAEDLGSARRVVATKDDTTFVDGQ
                     GAKEDVQARVQQIRSQIETTTSDFDREKLQERLAKLAGGVAVIKVGAATETELKEKKH
                     RVEDALSATRAAVEEGIVPGGGVTLINAVSALDTVQAEGDIKTGVNILRRALEEPLRG
                     IVRNAGRDGSVVIEMVRRLHEEKGDNNLGYDVIKDEYVNMLKAGIIDPAKVTRSAVEN
                     ASSIAGMILTTEALVSDKPEKEQPMPAMP"
BASE COUNT         4344 a         7181 c         7943 g         4137 t
ORIGIN      
        1 cggcgatcgc cgggagcgtc cgctcgacgg ccgcggcgac ggttgcgtcc tccgcgttga
       61 gtcggcggac gagatcgatg gtgggcaggg catcgatatc ttccgtgtta gggttccgct
      121 gctcggttga aagcttgccg aggtccttac cggtccgctg gtcggtcacc ttgctttctc
      181 ctgctggatc aacgggcggt agatgaggcc accgcccacg gaattgacga acgcgcagct
      241 ttgcatgcgc cagcaatcgc cgaggcgtca gtgttgccga cattatcgat tgtctcgttc
      301 aggtttgggg tgcgtccgag gagatccatg aagcgcgctt ctccaagtcg tgttcaggaa
      361 gagctcacgc tgcgacctcc caagctatcg ccagcgcctt cgggcccggc attctgatgc
      421 tcgggtggtg ggcgctcacg ggacgggtct cggctccgct gcagattctg ccctcgtgcc
      481 tgggaaagga tggcaagaag gtcattggtt ggaacaggta gccgactgat ccgccgatgt
      541 tcagtctgtt tggaggcagg attggcgatt cgtggcacgc cgatgttctt ggaacgacgg
      601 taccacgccc ttcggaacag gtcgttcctt gccaggcaag tggcggtgga ggtgcaaggc
      661 atcaagcgtc gggcaagccg gcgctttgac ccggccccat gcagcaggcg gcttacggag
      721 aaacggttcg agccctgcgg tcggggtcac gccctgctat acgccacttc ctggtaccgg
      781 gtgcgaagcg cctgctcgag ccgctcgtgt gcccgcctcg cgtccttccc gagatatagc
      841 gcatccaccg tatcccgaag ctccggctgg tcctcaaaca ccgccccgcc gtagccgatc
      901 aggggctcgg tgccggatgt ttcgatcgcg cgcaggcagg tgacggtatc ccggagcgtc
      961 tcgaccgaag gcggtgtcgg tgtggagagc accacagcat tcggtccaac gttgcgaacc
     1021 gcctcgacca gctcggtacc cggcaggttg gccccgaggt agatcacgcg gcagccacag
     1081 cgggagaaaa caagcgctgc gacgagcaag cccagctcgt gcatctcgcc ggcgacgccg
     1141 ctcatgatga ccgtagggct gccgcgctcc ggctctgatg cattgaagag gccggagagc
     1201 ttgcgcatgc agaacgaggt cgcgaagtgc tcgaccgcca tgcaggcctc accgcgctcc
     1261 cacattgtac cgagctcgac gagagcgggc tgcatgacgt cgagggtgac cgtctcgacg
     1321 tcatagattg ccagtgcctc ttcaacgatc tggttggcgc gttgggcgtc gaactcgaag
     1381 aaggcaccaa gcagcaactg ctggagcgtg tgcaagccgt tgtcgttgga gggtggggca
     1441 gttgagaaat cgtgagattc cgtgggtggt gcgttgctcg ccgaccggag ccgacggcgg
     1501 gccatggaga ccgcgcgact gatggagatt ccctgctcgg tcatctgctt gagccaggaa
     1561 atgatcgcga tgtcccgctc agagtaaagg cggtggtttc ctggcgtgcg cgcgggcgaa
     1621 ggaagcccgt gacgccgctc ccatgcccga aaggtatcgg ctggaacacc ggtcatggtt
     1681 tcgacggcac gcgtgttata ttcgggcaca gtctgctgtt caaagaggtc atccacaggg
     1741 acgcgttctc cttgctggac gcctgcatac gggctgtcga ccattcatcc tagaattgca
     1801 caccctatgg accgctactg atgtcgaggc tacatgccaa ccagcggcgc gcacggcgac
     1861 gtgaactcgt cgaccgatcc tggaaactgt cgaagtggtc acgagcagtg ctggtcggga
     1921 ttgtcgtgct gctggcggca agcgcctgta cggataatac cgacgatcgg cagtttgcgt
     1981 cggacccacg cacatcagag ccgacgccgg ccgcagaagt gcccgatgta ctgaccggga
     2041 cgccccgagc gcctgcacac ccgctggcgt cacccgagac cctggtcgat cgccgcggcg
     2101 cgccgtctgt cgtatacgcc gaaattgccg gaagttcccg ggcaatcacc acggacggcg
     2161 tcaaccgcct tactgacggg cccgttgcgg cgttcgcggc atcgccgagc ggcaaccagg
     2221 tcgctgtcgt cgttccgtcc gccacggggc ttgcctatga cgtgaagatc tacgcatccg
     2281 atggtacgct cctgcagcgc atcgatacag tgctcatctc gtctccgcct gctactccag
     2341 cagatcaggc gctcagcgag gacgaggctg tcgcgttgag ttggtcgcca cagggagggc
     2401 ggctgctgct ggcagatgtc tccggtcaac tgatcgacat ccgtctggac ggtaccgttg
     2461 agcagattca aacgcgatcg tcgctctccg ggctgattca ggcggaatgg tcgcctcgag
     2521 gcgatcagat cggggtgctc ttccgcgacg acgatggcta cggcagcctc gcattgatcg
     2581 acctatcgat agatcctgct tccgtggacg tgatcgcgcc ggtcggtctg gtttcgggag
     2641 agcctgcgtc ggtcgagagc ttcgcctgga acccttacgg tgacggcgtg ctctttcttc
     2701 aaggcaaccc gcccgatgat ggcgtgcgca atgggcaggt cgtaggctgg aaccaggcgt
     2761 ccaacacgac cgaggtgatc gcgaccggtg ggcaagtagg gccgactggg tcgatccgcc
     2821 aattcagcat cgcgcccgac ggcaaggcct tggcctatgc aatcgatatc cgcacgccgg
     2881 aaggctcgga tttcggtggg ctctacgtcc gctcgctggt acagccgcaa gtctaccgcg
     2941 tgccggtcaa accgaacgtg acggtgacgg ggttctggtg ggtggctgac gggctcgcgt
     3001 gggcaggcgt ggtcgaggca gagggcaaag cgcagacgtt ggtcgtgcgg tggattgatg
     3061 ggagtaggag gatccggaca ctcgccgaga ttcgcatggc gacgatggcg acgccggtcg
     3121 ctgctccgat gccgggggct ccggcagcca cgccggtacc catcacagcc gtggcttcat
     3181 ttgccactcc ggtagcggtg ccggcagagg gtgcaactcc agtggagtga cggtgcagga
     3241 aggatcgcag agggcgtcca ctacagcgtc cacccacggg ataacgatat cgacccctgg
     3301 cggcatctga ctcgagcgtc actcctcgtc gatgatcacc gcatcgaaga tgttcatcgc
     3361 cgccttgatt cgctcctcat cgatgtcgag tgaactgaca gccgagggcg tcgacgcgac
     3421 ggcgtcaacc acctcctcgt tgagtggatc gggcggtcgc acggtctgcg ctgccggctc
     3481 aggctcgctg tcagcgtcag gcgagggtgg ggtctcacta cccattgcct cggtgcgctc
     3541 acggggagtc ttgctctgca caccttgtaa agtggggtcc cggaccggcg cggcggccga
     3601 tgctctgggt cgcagggact gtgcctcgtc acgcgacacg cagagaaccg tcagcttctt
     3661 gccgacccgt cgctcgatca cctcctcgat gatccgtcgg gtgtcatcct cgttcagccg
     3721 cttctggtgg aatggatagg ccgcaacgag ggtgagctgc gacccttcga tgtgtagcgg
     3781 atcggcttgc tgcagcaggg cgtcggtgcg ccggttgaga gccttgatgt ccatgcggat
     3841 gttgtcccac cgctcgacga ccagctcgag gtctccgggc ggcaaagagg ccgtcgcttc
     3901 ggcaggcggc tcgaagccgg tctccgcggg ttccgcctcg gagatctggc ggcgcgatgg
     3961 ctctgtctgt tgctggggag tggctgagga tgtctcgggc gcttcctgct gtggcacctc
     4021 tggcgcggcg gagcgctgcg gcgctgcatc gcgaggcatg ctcgcgccgc gaacccgatc
     4081 cttgagcgat ggtcgggagc gctgtcgggg caccggcgct gactgctgct ccggtatgtc
     4141 gcctgtggga gtcgcgactg agacttgctg cggtgccgag caggcctcga cgagcgccag
     4201 ctccagcggc agctggacga tcgccgcatg ccgggccttg tagtcgatct cggcgaagat
     4261 cgtggcgacc cgcgcgagat cgttcagatc gaaccggtgc gccaacaccc ccgctatctc
     4321 gtcagcgtct gggctgccgg aggcgcgttc cagcatcagc acgcggaggt agccgatcag
     4381 ctgtcgtccg agttgccgga tatcctcacc ggcctcgacg gcatcattga tgatgcgcaa
     4441 tcccgcgccg gggtcgcggt cggcgacggc acgcacaagt ccttcgaccc gatcgttgcg
     4501 gctcaccccg agcactgccc gcacgagctc aacggtcact cgagggctct cgtcatcggt
     4561 ggcctccgag tagacggaga cttggtcgag gaggccgagt gcatcccgca ggctgcccgt
     4621 agcgtggcgg gcgatcgtcg cgatgccatc ctcgtcgatc tcgatgcctt ccagctcggc
     4681 cacttgccgt agccgtgccg acattgcgtc gatgctgatg cggcggaaat cgaagcgctg
     4741 gcaccgggac acaatggttt ggagcaactc ctcggggtcg gttgtggcga ggacgaagcg
     4801 ggtgtgcgcg ggcggctcct ccagcgtctt gaggaaggcg ttggcggccg caccggtgat
     4861 ctgatgggcc tcgtcgatga tatagaactt ggtcctgagg cttgtcggtg cgtacttgac
     4921 ccgctcgcgc agctcgcgga tatcgtcgat accacggttg ctggcggcgt cgatctcgat
     4981 cacgtcggtg gtggcatttg cattgatggc aaggcaggcc tcgcaccggt tgcagggccg
     5041 gttggtcgga tcggggtcca gacagctgac agccttggcg aggatccggg cggttgtcgt
     5101 tttgcccgtg ccgcgcggcc cgcagaagag ataggcatgg gcgatgcgat cgaggcggat
     5161 ggcgttgcgc aacgtgttga cgacgtggtc ctgcccgaaa agctcgtccg aggcgaacga
     5221 ctgtggccga tacttgcggt agagcgagag cgactgccgg cctgtcgcag gcaccacatc
     5281 cgggtcagcg gctgcggtcg atggcttcga gggctgggtt gcttcgacgt cgaacaggga
     5341 gtcggtcatg gttgcgtagg cctcgggatg tgttccggat gtctgcagtg gggccggaag
     5401 gtgtggatca tgctacctca gtatacccgt acggacactg tggctcaatg gaggagtaga
     5461 tgggaacgct gtacgtcgtc gccacgccgg ttggcaacct tgatgatatc tcgccccggg
     5521 ctgtgcgaac gctcgcggag gtctcgctga tcgcggcgga ggatacgcgg cacaccgcgc
     5581 ggctcctgag gcgctacgag atcgccaccc ccgtgttcag ctatcacagg cacaacgagc
     5641 gcgagcgggt ccagcggctg ctggaggcgc tcgtcgcagg cgatgtcgcc ctcgtctccg
     5701 atgccggcac gccagcggtc tcggatcccg gcgcggtgtt ggtttgcgcg gtccgcaaag
     5761 cggggcaccg cgtggtgccg atccccggac cctcgtcgct tgtggcggcc gtcagcgcta
     5821 gtgggttggt cgagggggcc ttcctgtttg gcgggttctt gccccgttca ggcgacgagc
     5881 gccggcgggc gatgcagcgg gcggccatcg tcgacgtgcc gctggtgctg ttcgagagcc
     5941 cgaaccggct gatacagacg ctcgacgacc ttcgtacgac gttcggcgat gtacaggcgg
     6001 tggtcgcgcg ggagctgacg aagattcacg aggagattcg ggccgggaga ctctcggagc
     6061 tggccgagca ttaccgttcg gcggaggtcc ggggggagat ctgcatcgtc gtggaggccg
     6121 ctccggcggt cgaggcggcg ggcggtaccg atgacactgc cgagattgcc gcgcggctgc
     6181 tggcggatgg aacgcagccg agcaaggcgg cgcgtgcgtt ggcgcgcatc acaggcatgt
     6241 cgagcggcga cgcgtatgag gtcattcgat ctgtgcggga gcaatccgac cggtaggggc
     6301 cggcctagcg ttgacctgat cgcccctgcg gcggggtgac gcccggctga cacaagcgca
     6361 gctgtgctga gcgcagcgag gcatcgatcc ccaaagtaca cgtgatgccc catgtctcct
     6421 atagacggat gaaggcgagc ttgcggtgat gcatccggat gctctggagg atgccgacga
     6481 gaatcaggtt tgaccagagc gaagagaggc catagctgac gaacggcaag gtgatgcccg
     6541 tcactggcat aacgccgaca ttcattccga tgttgaggaa tgcctggaag aacagcaccc
     6601 cggtgatacc cacggacagc acctggccga actggtcgcg cgcgatctcg gccaccttca
     6661 ggcaacgcca caggaagatc acgagcgcga ggaagagccc gaacataccg acgaacccga
     6721 acatcccgct cgcgtgcgcg aagataaagt ccgagtcgcg gacgctgagc aggttgagct
     6781 ggctctgcgt tccgccggcc agtccctcgc cccagagtcc accggcaccg atgctgatcc
     6841 tcgcctgcag gatctggaat ccctccgcga gtggcgcgac ctccggcgtg aacgagacct
     6901 ggaaacgggt tttctgatag tcctcgagaa tgaactgcca agcgatccag ccgagcagag
     6961 gcaggctgac gagcaccagc aggatataga gcgtccgagt gcgcatcacc agaagagcgc
     7021 tagcccatat ggccacatac accatcgtca tgccgaggtc cgggccgatc agcacgagtc
     7081 ctgccggcag agcgacgatc atcccggagg tcagaaagtt gatgaacgag cgcaactcgt
     7141 cctcgcgaga tgcgacgaac gaggcgaggg cgatgagtgt cgtgatgcgc acgaattctg
     7201 ccggctgcag caggaagaag ccgaagtcga accagttcct tgcgccgccc agctcgacac
     7261 cgaatgtctg cacggcgatc aacgcgagca caccgatgca gtagagcggc caggccaagg
     7321 tgttcaggaa gcggtaatcg atagaggcgg tgatcaggag gatcacgata cctacggcgc
     7381 cgaggagcgc ctggaaggtg ccgttgttga gggtggtcag gggcggcaag ccgaccgccg
     7441 accagatcgc catgataccg aaccccatca acaccagcat ggtgacgaag aggatcagat
     7501 cgaagtcgcg ccagcgatca cgggcggtgt cgcccggttt gacctgtatg tagatgggac
     7561 gaagcgtgac catgggcgtc cagtctgcat cgggtgtcgc gggcggccag gagcaggcgt
     7621 tacgcattca ccaccagcgg cggatgcgta acgcctgcgt gctattctag cccctaaccg
     7681 ggccggcatc cggttacttg tgttgcccgg tttcgtgcag gtagatggat ttgtatggct
     7741 gacgaacttt tctcctccgg catgccctct gatcgggacg tccagcgact gcgagtcgag
     7801 gcgctaggtg agccgggcca gcggcgcttc cggctgatgg cggtgatcaa cggtgaaacg
     7861 cacattgtct ggatggagaa gcagcagatg caggcgctgg gcatggcggt cgagcaactg
     7921 ctggagcagg tgccgcacag cgagatcccg gtgatcgaga gcaatgctgc ggtcgagttc
     7981 gaccagaata cgctgcacca gttccgcgcc ggtcgcatcg agctcggcta cgatcccacg
     8041 agcgagcggg ttgtgatcaa cgcgtacgat ctccagcagg aggacgagga cgcgcaggcg
     8101 acgctcgtgc tgcggctcaa cctgcggcag tcagcggagt tgagcgccga tgcggcggtg
     8161 atcgtcgctg ctggtcggcc actttgcccc atgtgtggcc agcccatgaa tccggaaggg
     8221 catgtctgcc cacagcagaa tggacacttg ccgatctctc tggatgacga cgacctcatg
     8281 gaagaattgt gattcgggaa atgctgctgg atgacgacga actgtaatcc ggagagtgtg
     8341 aagcggttga caatcatctc aggtcccgcg tacgatagga ccaagcaggc gatggcctgc
     8401 gcctgtcgaa gactgtgtca atcaacctga aggacatctc tcgtggctcc ttgccagatc
     8461 gccgacaagg tactggtact tatttcgctc ccgatcgtag ggagtatttg gtgtgcgggg
     8521 agacagtaac gccggcaggc gaaatcgaaa gaaaccgacg actggcctcc cccttgagtg
     8581 ggaggcattt ttgttgctcc tgaccaacgg cgtgaaggat gcagcccaac gtctcccaca
     8641 atccagcggc ggatgaacgg gaggcaagac gcagattggc gttggtgtca ccgatcccga
     8701 tccatcggtc taggtgacgg agagtggtcg ccggagctag attcacgcat cgtgtaagga
     8761 tggatcggtc agccccggcc gacccatcgg gaaatggagc aaactatggt cgcaactcaa
     8821 cgcccgcccg agtcgaccga gtctaccgac gaatcctccg tggcacgcgc ccccgaggta
     8881 ctcagcggtg cggagatcac ctgccgcagc atcgaggctg aaggagtctc gaaagttttc
     8941 ggctaccccg gcggtgcggt gattccgctc tacgacgcac tgccgtcgtc ttccttgaga
     9001 cacgtcttgg ttcgctttga gcagtgggcc ggtttggccg ccgatgcgta tgcccgtgca
     9061 acgggcgagg tcggggtgtg catcgccact tccgggccgg gcgcgaccaa tctggtgacc
     9121 gcgctcgcca atgccaatct tgactccgtt ccgatggtgg caatcaccgg gcaggtcggc
     9181 cagcagctca tcggtcgcga tggcttccag gaggttgaca tcaccggcgt ctcgctgccg
     9241 gtcaccaagc acaactatct ggtccagcgg ccggaggata ttgccccgac gatcaaggag
     9301 gcgttctacc tcgcccggag cggtcggcct ggcccggtgc tggtcgatat tcccaagaac
     9361 gtcttcgtca caaaggccga gtacaagccg ccggctcagg tgggccgcaa gagctaccag
     9421 ccgacaacca agccgcacgt ccggcaggtt cgcctcgcga tcgaggagat ggccagggcg
     9481 aagaagccgc tgatcatggc tggtcacggc atcattctga gcggtgccga ggcggagctt
     9541 cagcagttcg ccgagaagac gggcgttccg gtgatctaca cgctgctcgg gctcggtgcc
     9601 atgtcggaga tgcacccgct ctcgctcggc atgatgggca tgcatggcca ccgacatgtc
     9661 aatcgtgccc ttgaggagtg cgacctgctc atcaacatcg gtgcccgctt cgatgacagg
     9721 gccaccggcc ggctcaacgg gttttgcacc aatgcgcgga tcgtccacgt cgatgtcgat
     9781 cctgccgaga ttggcaagaa cgtgccgacg gacgtccccg tggtcgggga cgccaaggaa
     9841 tcgcttctca tgctgatcga ggaggcgggc gaggcgcagg actacagcgc ctggatgtcg
     9901 tggatcgacg gccagcgcaa ccgggtgctt gaggcggcgt tggaggatcg tcccgagtgg
     9961 ccggagccgt actcgatcgt caaggcgatc gccgaggcga ccgaccacga cgcgatcgtc
    10021 accaccgacg tcggccagca ccagatgtgg gccgcgcagc agctcgggat caagcacgga
    10081 aaacgctgga tcacctctgg cggtctgggc accatggggt atggccttcc atcagcgatc
    10141 ggcgccaaga tcggtcgtcc ggaccttgaa gtttggacga tcgtcggcga tggtggtttc
    10201 cagatgtcct tcaatgaact ggcgacagcg gttcaggaga acctggacat caacattgcc
    10261 atcatcaaca acggctacct tggcatggtg cggcagtggc aggatctctt ccacaaccac
    10321 aactattctg aagtgaggat cagcgctccc gacttcgtcc agctggctga gtcgttcggt
    10381 gtcaccggca tccgggttac gaccgacgac gagatcatgc cggcagtcga gcgagcgcgg
    10441 tccatcaagg gtccggtcgt gatcgacttc atcgtcgaac cggaagcgaa tgtctacccg
    10501 atggtgccgc cggggggcag caactcggag atggtccacg acccgcagtg gacgccgcac
    10561 gaggaggacg tctagccatg aagcacgccc acacactcgc tgtgctggtc gaggaccagc
    10621 ccggtgtcct caaccgggtg gtgtccctga tgcgccgccg ttcgttcaac atcgactcga
    10681 tcacggttgg gcactcggag cagccaggca tgtcccgcat gaccttggtc gtgcgaggcg
    10741 aggatgtcga ggttgagcag gtgaccaagc agctgtacaa gctgctggag gtgcagaagg
    10801 tcacagacct ctctgagttc gactccgtta cccgtgagat ggtgatggtg aaggtcgcgg
    10861 ccaaggggca gtcgcggacg gaaatcctac agctcgtgca ggtctataac gcgcgggtgc
    10921 tggacgccat gccgagtacg ctgatgatcg aggcaaccgg ggacgagcag cagatcgact
    10981 cgttcctggc gatgatgcgc gtttttggca ttcgtgagat ggtacgaagc ggcaagatcg
    11041 ccatgtcgcg gacctcgggc gccactcgcg ccaagggcga ggtcacagcg aatggcgtaa
    11101 gcactgccgc cgattgatgg aggacgagtg gagacgggag gccgcaagga cctcccgttt
    11161 ccatgagcgg acgatatcgc tggcccgtgg aggctgcggg acacgaacgc aacgagggcc
    11221 gggagagccc gaggaggatt caatgccagc aacgatctat tacgagaccg acgcgacgct
    11281 tgcgccgctt caggacaaga cgattgcgat catcgggtac ggctcccaag ggcgcgcgca
    11341 cgcactcaac ctgcgcgatt ccggtctcag cgttgttgtc ggcctgcacg agggcagcaa
    11401 gagccgagcc aaggcggagg cggatggcgt ccgggtcgag tcggcggctg atgcggcgaa
    11461 ggaagccgac gtcatcatga tgctcgttcc ggaccagacc cagaagaagg tttacgagga
    11521 gtcggtcaag ccgcacctgg acgtgagcaa gaccctcatg ttcgcgcacg gcttcaacat
    11581 ccacttcaag caaatcgaat cgccccaagg cgttgatgtg accatgatcg cgccgaaggc
    11641 cccgggcacg cgggtccggg agctgtacga ggagggcatg ggcgttccgg ctctggtggc
    11701 cgtccaccag gacgccagcg gcaatgcgaa ggagcaggcc ctctcctacg cacgggcgct
    11761 tggctcgctc agcgcgggcg tgcttgaaac ctccttcgag gaggagaccg agaccgatct
    11821 cttcggagag caggcggtcc tctgtggcgg tgtctcggcg ctgatccaag ccggattcga
    11881 gaccctgacc gaggccggct accagccgga gagcgcctat ttcgaggtgc tccacgagct
    11941 gaagctcatc gtcgacctca tctacgaggg tggcctcacg caaatgcgcg aggtggtttc
    12001 cgataccgcc gagtacggcg actactacgc tggtccgcag atcatcaccg aggacacgaa
    12061 gcaggcgatg aagaacctgc tcggcaagat tcaggacggc acgttcgcgc gggagtggat
    12121 cgccgagaac gaggccggcc gcgagaactt catgcggatg cgccaggaac acactgacca
    12181 cgagatcgag cgtgtcggca ggcagctccg cgccatgatg ccgtggctca agagcggcaa
    12241 ggctgcccgc gaggatgcca aggcggcgat gagcgcatct cagtagcggc ggacctcgtg
    12301 tccgcctggc tcagcgcgat tcccgccgat ccgtagtatc gggccaaccg gctggggaga
    12361 ggatcatcat gagcgatcga gagagcaatg caggtgtcga gcagtcccgt gtcatcgttt
    12421 tcgacacgac gcttcgcgac ggcgagcagt cgccgggcgc gacgcttacg accgacgaga
    12481 agctcgaggt ggcagatgcc ctcgtggctc tcaacgtcga cgtgatcgag gccggattcc
    12541 ccgccgcatc gccgggcgac ttcgaggcgg ttcgcgagat tgcgcggcgt tcgaagggcg
    12601 tctccatcgc cggtctggcg cgcgccaaca cgagggacat cgagcgtgct gcggcggcag
    12661 ttcacgaggc agaggcaccg cgcatccaca ccttcatcgc cacgtcggac atccacctga
    12721 agcacaagct gcggatgacc cggcaggagg tgctggagcg ggttcgagag atggtggcct
    12781 tctccaagag cttcgtgagc gatgtcgagt tctcctgcga ggatgcgact cgttcggact
    12841 gggactacct cgtgcaggtc ttccaggcag ccatcgaggc cggtgcgacg accatcaacg
    12901 tgccggatac cgtcgggtac acaacaccca acgagtttgt cggactgatg caccaccttc
    12961 ggtcgaatgt cgatcgcatc gacgatgtga ttatctcggt tcactgccac aacgacctcg
    13021 gtatggccac cgccaacacc ttggcagcgg tcaagaccgg ggcacggcaa gtcgaggtaa
    13081 cggtcaatgg catcggcgaa cgcgccggta acaccgcgct cgaagaggtc gtgatggccc
    13141 tgcagacacg gggcgagcag ttcggcggcg tgcacaccaa cgtcgagacg acgcggctgg
    13201 taccggcaag ccgcttggtc tccggcctga cgggtctgca ggtccaagtc aacaaggcga
    13261 ttgtgggcgc gaatgcattc gcacacgagg cgggcatcca ccaggacggc atgctcaagg
    13321 agcgcacgac ctacgagatc atgaacccgg aggatgtcgg atgggaaggc acgcgcctgg
    13381 tgctcggcaa gcactctgga cgcgctggct tcaagaacgc actcaaggaa ctcgggctgg
    13441 tgctcgatgg ggatcaggtg cagggcgtct acgagcgctt ccttgagctc gccgaccgca
    13501 agaagaaggt taccggcgcg gacatctcgg cactggtcgc cgacgaggtt gcgggcgagg
    13561 aaggccgtta cgaactggtt cgctggaacg cggcgatcgg caccggtggt gctgcgactg
    13621 cgtcggtggt cctctcacag gatggccggg agatcgctgg cgacggcgag gggaacggtc
    13681 ccgtcaacgc tctctacgcg gcgatcgaca acgcggtgca gatcgggaac gagctggagt
    13741 actaccacgt cgaggccgtc acccccggag aggacgctca gggccaggtc catgtgcgta
    13801 ttcgcgtcgg ccagaatctc tacagcggtc acggcctcgc cacggacatc gtcgaggcga
    13861 gcgccaaggc ctacctgtcg gcgctcagcc gtgtcgatca agatcagccc gacacggtgc
    13921 tcatgggctc gtcgacctcg cgctggagct aggccgtgcg cgaaccggat gtccccgatg
    13981 cgtcccggcg tgacgtgatc tggcgccgag acgataccgt ggcgaattac ctcggtgcca
    14041 cccggcaggc gatcccgctc gccgccgagc agctcgatgc cgcgatgcgg gcgattggcg
    14101 ccttcgccgt cccgacccgc tcggtgctcg acgtcgggtc gggcggcggc tcggccgcgg
    14161 cggcgatctc ccagtgcttc ccggtggagc gcatcacgct cgtcgacttc tcaatgccga
    14221 tgcttcggca gaggatcgtc cgcttcgagg gcagcccctg gctcgtcgac ctgatcgaag
    14281 ccgatctcca ggatcccgcc tggcaggatg agttgccggc ggaccagcca gcatacgacc
    14341 tggtggtttc gcgctacgcg atccaccacc tgcctgacga ccggaagcag gatctctacg
    14401 ccgagatctt cgggcgcacg gcaccgggag gactgttcgt gaacatcgag cacgtctcgt
    14461 cggtggcgcc ggtctacgag gacattttcg agagtctgat cgtcgaggga atggcggcga
    14521 cgagcgagac aggtcaggac ttggccgccg cgaccaccgc cttccacgcc cggcaggacc
    14581 gggaaaccaa cattcttgct cccgctgatc tgcaatgtgg ctggctgcgg gatactggct
    14641 tcgtcgacgt caacgttttg atgaagacct tcgaacttgc ggtcatcgtc ggccgcaggc
    14701 cgaatatgta agggcggggc atcgtcgtgg aaacgctcta tacgagcgga ctccgtgtcc
    14761 gcccgacgaa tgacccagcc gtcacatgga ggaatccggg tgatcatgcc caagacgctc
    14821 gctgaaaagc tgtgggaccg ccacatggtg cactcggcac cgggcgagcc cgatctgctc
    14881 tacatcgatc tccatctcat ccacgaggtg acctcgccgc aggcgttcga cgggctccgg
    14941 ctcgccggcc gcgccgtgcg ccggccagac ctcaccgtcg gcaccgccga ccacaacgtg
    15001 ccgaccatcg gcatcagcct gccagtcgcc gacccgatct ctcgaaagca gctcgagaag
    15061 ctgaccgaga actgccggga gttcggcatc gagctctacc cgatgggcca cccggggcag
    15121 gggatcgtgc atgtgatcgg tcctgagttg gggctcacgc agccgggtat gacaatcgtg
    15181 tgcggtgaca gccatacctc gactcacggt gccttcggtg cactcgcctt cggcatcggc
    15241 acgagcgagg tggagcacgt gctggccacg cagacattgc cgcagagcaa gccgaagacg
    15301 atggcgatca ccctgaatgg cgacctccag aacggcgcaa gtgccaagga tgtcatcctc
    15361 gccatcatcg gcaagatcgg cacgggcggc ggcatcggct cgatggtgga gtatagggga
    15421 tcagtcatcg agagcctctc gatggagggc cgcatgacga tctgcaacat gtcgatcgag
    15481 gcgggcgcca aggccggcat ggtcgcgccc gacgacacga cctatcgtta cgtcgaaggc
    15541 cgaccgcacg cgccaaaagg caagctctgg gagcaggcgc tagaggactg gcagtcgctc
    15601 cggactgacg aggacgccga gttcgaccac gaggtggggc tggatggcag caccatccag
    15661 cctcacgttt cctggggtac caacccggga caggtcgcgc ccatcgacag ccgcgtcccc
    15721 gaccccgact cctaccaatc cgccagcgat cgcaatgccg ccgaacgtgc gctgcagtac
    15781 atggcgttga agcccggcac cgcgatccgt gacatcgcag tcgacaaggt cttcattggc
    15841 agctgcacca actcgcgcat ggaggatctg cgtgccgcgg cggagatcgt cgcgggccag
    15901 cggctcgcga acaacgtgca ggcaatggtg gttcccggct cgatgcgcgt gaaggcacag
    15961 gcggaggcag aggggcttga ccgcatcttc acagatgcgg ggttcgagtg gcgcgctgct
    16021 ggctgctcga tgtgtctcgg catgaacccc gaccaactgc agccgggcga gcgttgcgcc
    16081 tcgaccagca atcgcaactt tgagggccgg cagggcaagg gcggacggac gcatctggta
    16141 tcgccacagg tcgccgcagc gaccgcgatt gccggtacct tcgccacccc gaccgacatt
    16201 ctggcaggag atcgcgcatg aacccgattg agacgatcga aggccgcgcc gtcccgctgg
    16261 atcggagcga cgtcgatacc gaccagatca tcccggcgtc gtacctcaag cgggtggagc
    16321 ggacggggtt cggagagggc ctcttcgcga gctggcggga gaacgacaag aatttcgtgc
    16381 tcaaccgggc cgagttcgag ggcgcgagca tcatggtcgc cggcccgaac ttcggtaccg
    16441 gatcctcccg tgagcacgcc gtctgggcgc tgacggatta cggcatcaag gcggtgatct
    16501 gcccgcagtt cggcgacatt ttccgcaaca acgcaacgaa gagcgggctg gtaccggtgc
    16561 aggtcgatcc cgacttcggc gccgagttgc ttgaggcgac ggccgagcat ccgtttctcg
    16621 agatcaagat cgacatccgc aaccggaagg tctcggcacc ggcggtcgga ttggaggcaa
    16681 ccttcccgct tgacgagttc acccagtacc ggctgctgga ggggctcgac gacatcgggc
    16741 tgacccttcg caacgaggac aagatcagcg agttcgaaca gcaccgaccg gcgtacaagc
    16801 cgcgcctcga gacggcggct gcacggtagt cagtcgaagc aaggagcagt cgcaggggcg
    16861 gaccccggag gggtccgccc gttttcctga aagggaggtt cgagtgcagg cgacgatagt
    16921 gacgttcccg ggcgacgggg tcgggccgga ggtaatgggg caggcgcttc gcgtgttgca
    16981 ggctgttgct gagcagaacg ggcatgcgtt cgctttcgac gaacggaaga tcggcggtat
    17041 cgccatcgat gcctacggca ccccgctgcg cgacgaggag atcgaagcgg cccgagatgc
    17101 cgacgcgatc ctgctcggcg cggtgggcgg tcccaagtgg gacaacccca atgccgcggt
    17161 tcgtccggag caaggcttgc tcaggattcg caaggcgctg cgcctctttg caaatctgcg
    17221 tcccgtggtg ctgcaggaat cgctgatcgg cgcctcgccg ctccgcccgg agcgcgtgaa
    17281 ggggaccgac ctcgtcgtgg tccgcgagct tactggcggt ctctacttcg gcaagccctc
    17341 gaaacagtgg cgggagcgcc ggggccaggt cgcggtcgac acgttggtct accgcgagca
    17401 cgaaatcgag cgggttgtcc gcttggcgtt ccaactcgcc caaggtcgat cgaagaaggt
    17461 cacgagcgtc gacaaggcca acgtgctgtc gtcgtcgcgg ctctggcgct cagtggtcac
    17521 cgaagttgcc gctgagtacc ccgatgtgca actggagcac atgctcattg atgcgatgac
    17581 gatgagactg atccagacac cgtcgagttt cgacgtgatt gtcaccgaga acatgttcgg
    17641 tgacatcatt acggatgagg cctcggtgct gggcgggtcg attgggttgt tgccgtcggc
    17701 gagcctcggt gccgagcgcg agggacagcg gcggaaggga ctctacgagc cgatacatgg
    17761 cagcgccccc gatatcgccg ggaaggggac ggccaacccg gccggaatga tcctttcggc
    17821 cgcgatgatg gttaggctct cgctcggtct tgagcaggaa gcggtgtcgg tcgagcgggc
    17881 cgtggcgagc gccatcgacg atggcgtgcg aaccgccgat ctgggtggtg atgccagcac
    17941 ggagatgttc ggcgatgccg ttgtccaacg gctctgaccc aaggaagaca gcgggaacca
    18001 gggcggaaac acgacgagga tgtaagggac ggataaacgg cggcctgtct tcctgcctgc
    18061 tcagacgaat acgtccgagg gacctctctg ggtgatggtt tcggcggggc cgagcgaatg
    18121 gcctagatcg aatctgtgga gaatctccgg ctccgagaat ggatcagccg tcgcgatggc
    18181 cagcagctcg agtgcctcgg cgtgaaacat cacgaacgtc cggtcgcgcc agcgtgccat
    18241 tcgagctggg aagccccgca tcgtctcgtc tcgcgtgtag tacccgagcg ggacgtgagg
    18301 gaggtaggcg taatcgtcgg ccaccggcag gagcgtgaag tcacggagcc ggtgatgcat
    18361 cctgaagcac cagccgtcgt cgtgtacgtc gagaaacggg gtatcgagga aggagttgaa
    18421 ccctcccact ctgatttcga agcgcggaaa atcgcagacc ggcgtgctgg aatggtgaac
    18481 gaactcttcg atccgttcct gagaaatttc gtcgatccgt tggggctcgt caaccaggaa
    18541 gccgagctcc tggatcgtga ttgccagcga ctccgcgggc gccagcctga cgaacggaaa
    18601 ctccgacagt gccgcgcgca gcggcttgag cgcgctggcc gtcttgtccc gaggcaagcg
    18661 gatcgcgcag accacaaagg tgtcggcgcg atcctggatc ttttcactgc caagcgtgac
    18721 ggcctgcacc tgaccgtgct gtcgaagcgc ctgccagatg tttcggaagg cctcgtcctg
    18781 acgggcagag accggtggcc accacgctgc cggccctcga aggcgcagcc gccggcgggg
    18841 aggccgcggc agagtggtgc gaatagggcg cggcatggcg gcttcctggc ttaggagttg
    18901 tccacggagc ggatggtgat atcgaggtgg ccgcccgggg tgctgaccga taccgtctca
    18961 ccggcgcgcc gcccgtcgag tgccttgccg atgggcgagg cgttcgagat gaagccggca
    19021 ctcgggcgag cctcggctgc gccgacgatc gtgaacacct cttcctcacc ttccatgtcg
    19081 acggcgacct tgctgccgag cgtgacagtt gaacgctttc tgttcgccgc atcacgagcg
    19141 accacagtcg cgttgcggag ggttgcctcc aggtcggaga tacgcttttc gatcatcgcc
    19201 tggtcctggc gcgcggcgtc ataggccgag ttctcgcgca ggtcgccgtg cgagcgggca
    19261 tcggcgaccg cctcgatgat cttcgggcgc tcgacctcga tcagctggcg caactcctcc
    19321 tgcaggcgct cgtgcccctc ttcggtaatc tcgattcgtt ccttgtccat cgggcggata
    19381 tctctccatc caagcgcgcc acgtcgcccg tggcgggtga tcgttcctga cttcggcaat
    19441 agggaaacgc cgggatagcc acccggcgtt tcaaccatgc tccgacccac aaggatacga
    19501 tacccgtcaa gttgcggtgt gccgccctgc gcccgacggt gctactccac ctcgaaagtc
    19561 gcctcgattt cgacgcagat gttgtttggc agcgcggcga aaccgacggc ggaccgggtg
    19621 tggcgtcctt tctcgccgag gacctcgacc agcgtgtccg agcatccgtt gatcaccctg
    19681 ggctgctcgc cgaagtcggg tgcactgttg accatgccga gcaccttgac cgtcctggtt
    19741 atgcggccga ggtcaccgcc gagcgcctgc ctggcggtcg cgaggatctg cagcgcgacc
    19801 tcgcgtgcgg cttggtaggc gtcctcgacg ctcacgtcgg cgccgacctt gcccttcggt
    19861 gaggcatctg tcgggccggt cccggagatg aacagcaggt tgccggtctg ctggtggcgg
    19921 acgtagttgg cggccgggct caccggttcc ggcagctcga tccccagttc cttgaggcga
    19981 tcatcaacgc tcatgggtcc tcccttgtca cttcgctcag ttcgtagcta ccgcatcagc
    20041 gcgtcccgaa gagcctggtg agccgctcga tggtcgacga gagggcatcg tcgctggtgg
    20101 cgaaactcaa gcggacatgg ccctcgccgg cctccccgaa ggcgatcccg ggcaccacgg
    20161 caacgtgcgc ttcctcgagc atcttcgccg ccagatctgt agaggacaac cccgtctcgc
    20221 gaccatcgac aaaggcgtag aaggcgccct cggccggcat ccacgagagg ccgttgatcc
    20281 cattgacgcc ctcggtgatc agcttacggc ggcgatccca cgcctgcacc atctcgccga
    20341 tcacgtcctg cgggccctcc agggcggcga caccaccagc ctgggcaaaa gaggtcgcgc
    20401 aagtgactgc gtgaccatgc acggtcgcga gtttcgatat caacggtcgt ggaccggcca
    20461 cgtaacccag acgccagccg gtcatggcgt aggccttgga gaggccgttg aaggtaagcg
    20521 tccgctcctt catgccgtcg aaggtggcga tgctgacgtg ctcgtgatcg tcatagacga
    20581 tcttctcgta catctcgtcg gtccacacca gcagatcgtg gcggttggcg aacgtgacaa
    20641 tggtcttgag ttcctttcgg tccatgacat ggccggttgg gttattcggc gagttgatca
    20701 acagtacccg cgagtttggt gtgacgtgcg cctcaagtgc ctgctccgtg acgacccagt
    20761 tgtcctcagg actggtggga accgcgacga cgttcccgcc ggcaagctcg atctgcgggg
    20821 cgtaggacac ccaggccggt tcgaccacga ggacgtcgac gccgggctcg acaagcgcca
    20881 ttgcggcctc gaagagcgca gccttgccgc cgggggtgac gaggatctcg tcgttggggt
    20941 cgtaggcgag cccgttgtcg tcgcggagct tgctggcgat cgccttgcgg aaggcggggg
    21001 tgccagggct ggcgacataa tgtgtctcgc cgctgttcat cgcctcggcg gccgcatggc
    21061 ggacatgctc gggcgtgatg aagtcagggt ccccgccgcc gagatcgatc acgtcaattc
    21121 cctctgcctt gagttgccgc gccttgtcgg aaacggcaag ggtgggcgag ggggtggcgt
    21181 ttttcacgcg gtcggcaaga aagtctgctg gctggcggcg tgtctccatt ggtccttcct
    21241 catgactcta ccacggtcgg cgtgctggtc gcgtcaacgt ctaccttgcc tcagtgtacc
    21301 ggttccgaat gctcaggaca ggcggacatt tcacgcgtgg tggctttgag aaagacccta
    21361 ggggtgtcag ctccgtgggc ggaacggacc tggacggcat ccgtgccagg aggagacggc
    21421 gtatattcat gttgaagcgg gctcgttggg tctccagtag ttacccgggt tagcactcat
    21481 atcatgctga ttgccaaatt gctggcactc cacccttgca gagtgctaat cactgtgcta
    21541 gtctgaggtc gcaatcgaga tccgggccgc cgcagggtcc ggattcgtga tgtcgaggag
    21601 ccttgacggc actagcgcat tgtttcagga ggattgaagg accatggcga gtaattcact
    21661 caagcctctc ggtgaccggt tggtgatcaa gcctgcctcc cgtgaagagc agaccgcaag
    21721 cggcatcgtg ctgccggaca cggccaagga gaagccgcag cgcggcaccg tgatcgccgc
    21781 tggcgacggt cgtcgtgatg aagacggcga tcgcatcccg atggaggtga aggtcggcga
    21841 cgaggtgatc ttcgccaagt acgccggcac cgagttcaag ctcgacgaag aggatgtcct
    21901 catcctttcg gagaaggacg tgctggccgt catcggctag ccgttcgatt ctcatccgtc
    21961 tctgacgaca ggcgtttcaa tcaggaggaa tcactgtggc aaaggtactg gaattcaacg
    22021 agcaggcgcg acactcgctg aaggagggcg tggatacgct cgccaacgcg gtgaagacga
    22081 cgctcggccc gaagggccgc aacgtcgccc tggacaagaa gttcggcgcg ccgaccgtca
    22141 cccatgacgg cgtcaccgtc gccaaggaga tcgagctgga ggaccacttc gccaacatgg
    22201 gtgcccagct cctcaaggag gcggcgacca agaccaacga cgccgccggt gacggcacca
    22261 ccacggccac cgtgctcgcc caggcgattg tgcacgaggg catgcgcaac gtcgccgccg
    22321 gcgcgaacgc gatgatgctc aagcagggca tcgatctggc gtccgacgcc atcgtggccc
    22381 gtatccgcga ggtggccacc gaggtcaagg acaaggagga gatctcccag gtcgccgcga
    22441 tctcggctgc cgacaaggag atcggtgacc tcatcgccga ggtcatggac aaggtcggca
    22501 aggacggcgt gatcacggtc gaggagtcca ggggcctcgc gttcgagacc gagtacaccg
    22561 agggcatgca gatcgaccgc ggctacatct ccccgtactt cgtgaccaac acggagcgca
    22621 tggaggcgag cttcgaggat ccgttcatcc tcatcaccga caagaagatc tcctcgatcc
    22681 aggatattct gccgacggtc gagatggttg cccgccaggg caagggcctc gtgatcatcg
    22741 ccgaggacgt cgacggcgag gcgctcgcga cgctggtggt caacaagctt caaggtcgct
    22801 tccagtcgct ggccatcaag gcaccgggct tcggtgaccg ccgcaaggag atgctccgcg
    22861 acatcgccgc gttgaccggt gcgcgggtaa tctcggaaga ggtcggccgc aagctcgaca
    22921 gcgtgactgc cgaggacctg ggctccgccc gccgcgtggt cgccaccaag gacgacacta
    22981 ccttcgtcga tggccaaggt gccaaggagg atgtccaggc gcgcgtccag cagatccggt
    23041 cccagatcga gacgacgacc tcggacttcg accgcgagaa gctccaggag cggctggcga
    23101 agctggctgg tggcgtggcg gtgatcaagg tcggcgccgc gacggagacc gagctcaagg
    23161 agaagaagca ccgtgttgag gacgcgctga gcgcgacccg cgccgccgtc gaggagggca
    23221 tcgtcccggg tggcggtgtg acgctgatca acgccgtcag cgccttggat accgtgcagg
    23281 ctgagggtga catcaagacc ggcgtcaaca tcctccggcg cgcgctggag gagccgctgc
    23341 gcggcattgt ccgcaacgcg ggccgcgacg gctcggtcgt gatcgagatg gtgcgccgcc
    23401 tccacgagga gaagggtgac aacaacctcg gctacgacgt gatcaaggat gagtacgtca
    23461 acatgctcaa ggcgggcatc atcgacccgg ccaaggtgac ccgctcggcg gtcgagaacg
    23521 ccagctccat cgcgggcatg atcctcacca ccgaggcgct cgtctccgac aagccggaga
    23581 aggagcagcc gatgccggcc atgcc
//
LOCUS       DASYWL010000353         7790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_131038, whole genome shotgun sequence.
ACCESSION   DASYWL010000353 DASYWL010000000
VERSION     DASYWL010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7790
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131038"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..1167
                     /locus_tag="VGR22_12440"
     CDS             232..1167
                     /locus_tag="VGR22_12440"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEV2129418.1"
                     /translation="MRIAAFDGGKPGIVASDDTVVEIGDVLAQYDPLGPENYIPDLIT
                     HYEELRPELERRAEQGGGTPLDQVQLDAPFARPGKIICLMGNYREGTDRPKQRIDLFF
                     KSPESLVGDRGTVMLPPHQADIFHHEAEIAVVIGKEAKSLSDDEAMDAVFGYVAFNDV
                     SARGLGRSEINSFLGKSFDTFGGFGPWIVTKDEIPDPQDLHITVDVDGERRQDYHTSD
                     MERPIKELMTYISSITTLHPGDVICCGTNHQGLGSMQDGDSVTTTVEGIGSFTLRVKD
                     SAARAWQRGVDKATAERVKAQAVHPDVPEAGGSGS"
     gene            1164..2057
                     /locus_tag="VGR22_12445"
     CDS             1164..2057
                     /locus_tag="VGR22_12445"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEV2129419.1"
                     /translation="MRLVLTKDDRIGVLVDNGMVDVSTAFEDLRYRTAADRMPRVMAV
                     LKERRDKVEELAASGTPEAVPALQAPVPRPPKVIAAFGNYREGWDRERQPQDMFLESP
                     DSVTGPDSTVVLPDHPADIFHHEAELALVIGERAKDLPADASAMDCLAGFTCAMDVSA
                     RGIGRVGPSRIGKSFDTFTPLGPAVVTPDEVLDVQNLRVTLHVNGDQRQDYTTADMEY
                     TVPEVLHFISSYMTLVPGDVILCGTQHQGLGALQDGDHAEMTIEHVGSLAVDVRDPLG
                     REWPRGIDEEMAARQRAVGQS"
     gene            2149..3810
                     /locus_tag="VGR22_12450"
     CDS             2149..3810
                     /locus_tag="VGR22_12450"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2129420.1"
                     /translation="MTQRKMTSTPPRIQGSRMSRRRAVQAGMAGAAVLAVAGQVGGAS
                     ARGVGTARLMRQDGEPQRGGRMVIASTGQPAGLDVQLFNNRTVTLIAWNMHEALFTFD
                     GDYATVPMLAEGIEVSEDGLTNTITLRQGVPFHNGEEMVANDVVASFNRWMPISSLGQ
                     AIQPFLESITAVDDYTVEIVLTTPLVALPSLLARQSQGLAIMPASIMENAGGEPLATE
                     DYIGTGPYRFVEFQADRYTLMERFEDYASLDTEHSGYAGAKPAYLDELEFVPVPDEAA
                     RLAGLQSGEYHYLEEIIPDQIEAVGGDPNVTIEILPPRSFGVVTLNNAGGLTTDVAIR
                     RAIQAAVSVEPLAQASHGEGYFEPGPGIMLPQTAWVSEVGSELYNQGDPEKCQQLLDE
                     AGYDGTPIRIITTQEDLGDYNAAVVLQQQLEEGGFTVELEVFDEATLDDNLEEEEGWE
                     ITTDSIVFRPDPALINAYASCTADAQWCSDEKVAIMERIQTEQDPEARFAAFEELQQL
                     WYDEVPMVKITDQFGVAALASNVQGLLGTMHFEIEPEFANAWLSE"
     gene            3966..4916
                     /locus_tag="VGR22_12455"
     CDS             3966..4916
                     /locus_tag="VGR22_12455"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2129421.1"
                     /translation="MIAYIIRRLLLLIPVLIIVAVIVFSLVHLTPGDPTSAMLGREAS
                     AEDRAALREELGLNKPLLKQFVDWSWGAMRLDFGESFFIRKPVTEALLDRAEPTGLLT
                     LYSLTFAVALALPSGVIAAMRPNSLIDRLLMVTSISGAAIPSFFFGILLMLFFAVNLG
                     WLPSGGYQDIGNGFWQHFRYMIMPSVALGFSSAGLLARIVRSTMLDVLNQEYIRTAYA
                     KGLAENQVIRGHALRNALIPAMTVTGIMLANMLGGAVVIETVFNIPGMGRLLVQSVVR
                     RDFPVVQAAVLVVAVIEVLVMLLIDILYVFVDPRIRYGSE"
     gene            4918..5826
                     /locus_tag="VGR22_12460"
     CDS             4918..5826
                     /locus_tag="VGR22_12460"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2129422.1"
                     /translation="MDLASNATAPKRARRFTPYAIPSNSKFYWMRLVAENPAAVGSLI
                     LLLIIALGATFAPVITQFDPDYVNPADRLSPPSSTYWFGTDDLGRDIYTRVVYGARVS
                     LLVGLAVMVGATTIGALLGLIAGFYPRRIDTPLMRVMDGLEAFPSILLAIAIMAAFGA
                     AVQNVIIALSVVYAPNIVRLVRGTTLMNSRELYVESARAIGMRDWSIMHKYILRNSLS
                     PLIVQATFVVAFAILAEASLSFLGAGVPQDIPTWGGMVSAGQARLQQAWWAAVFPGVM
                     LFITVLAINLIGDALRDALDPRSRQR"
     gene            complement(5839..6318)
                     /locus_tag="VGR22_12465"
     CDS             complement(5839..6318)
                     /locus_tag="VGR22_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129423.1"
                     /translation="MADSEKAGVDGTVTRESVDNSDAVIEAILEEASDDPKVVLIRLS
                     LEIEKELRFLLGSAGFLQDRDYLPLRKAVETLWHEYPVLPPTVIDAIEAFWEVGNHTI
                     HGGKGDRDAVVRAVDSGLVVLKTLHGLPMVQFVVYHPGVEVYADAAGQQVRQDVKKL"
     gene            complement(6579..6704)
                     /locus_tag="VGR22_12470"
     CDS             complement(6579..6704)
                     /locus_tag="VGR22_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129424.1"
                     /translation="MKFMDATQDQSFMASCQLGAATIADGFDGELLIMETGGQFR"
BASE COUNT         1457 a         2448 c         2434 g         1451 t
ORIGIN      
        1 gcaccgaggg tgacaagcgc gcggcggcca atgttctccc cctcgaccta tcccacatcg
       61 cattttccgt gaaatcggca caccggtgtc tgacgttcga gagtcagcca taattagata
      121 ctttctgatg acatttgcag aagcctattg acacgccgat tgccgcgtga caccatttcg
      181 ctacttgcac aattgagctg ctcgccgcgg caaggagtga caggaggcaa gatgcgtatc
      241 gctgcattcg atggcgggaa acccggcatc gtcgcgtctg acgacaccgt ggtcgagatc
      301 ggggacgtgc tggcgcagta tgacccgctg ggcccggaga actacattcc ggatctcatt
      361 acgcactacg aggagctgcg cccagagctg gagcgccgcg ccgagcaagg cggaggcacg
      421 ccgctcgacc aggtccagct cgacgcgccc ttcgcgcggc cgggcaagat aatctgcctg
      481 atgggcaact accgcgaggg caccgaccgg ccgaaacagc ggatcgacct cttcttcaag
      541 tcgccggaga gcctggttgg ggaccgcggc accgtgatgc tccccccgca tcaggccgac
      601 atcttccacc acgaggccga aattgctgtg gtcatcggca aggaggcgaa atcgctctcc
      661 gatgacgagg caatggatgc cgtgttcggc tacgttgcct tcaacgatgt ctcggcgcgc
      721 ggcctcggcc gctcggagat caacagcttc ctcggcaagt cgtttgacac cttcggtggg
      781 ttcggtccgt ggatcgttac gaaagacgaa attcccgacc cgcaagacct gcacatcacg
      841 gtcgatgtcg acggcgagcg ccggcaggat taccacacca gcgacatgga gcgcccgatc
      901 aaggagttga tgacctacat ctcctcaatt accacgctgc accccggcga cgtcatctgc
      961 tgcggcacca accaccaggg tctcggctcg atgcaggacg gcgattcggt gacgaccacg
     1021 gtcgagggta tcggttcgtt tacgctgcgt gtgaaggatt cggcggcgcg agcgtggcag
     1081 cgtggcgtcg acaaggcaac ggcagagcgg gtgaaggcgc aggcagtgca tcccgatgtg
     1141 cctgaggcag gaggatcggg ttcatgagac tcgtgctgac gaaggacgat cgaatcggcg
     1201 tgcttgttga taacgggatg gtcgacgtct cgaccgcctt cgaagatctc cgctatcgca
     1261 ccgccgcgga ccgcatgccg cgcgtgatgg cggtgttgaa ggagcgacgc gacaaggtcg
     1321 aggagttggc cgccagcggc acgcccgagg cggttccggc gcttcaggcg cccgtgccac
     1381 gaccaccgaa ggtgatcgcc gcgttcggca actaccgcga ggggtgggac cgcgagcgcc
     1441 agccgcaaga catgttcctg gagagccccg actcggtcac cggcccggac agcaccgtgg
     1501 tactgccgga ccaccccgcc gacatcttcc accacgaagc cgagctggcg ctcgtgatcg
     1561 gcgagcgggc gaaggacctc cccgccgacg cctcggcgat ggattgcctc gccgggttca
     1621 cctgtgcgat ggatgtctcg gcgcgcggca ttggccgtgt cgggcctagt cgtatcggca
     1681 agtcattcga cacatttacg ccgctcggcc cggccgtggt gacgccggac gaggtgctcg
     1741 acgtgcagaa cctgcgtgtg acgctgcacg tcaacggcga ccagcggcag gattacacca
     1801 ccgccgacat ggaatacacc gtgccggagg tcctgcactt catctcgagc tacatgacgc
     1861 tcgtccccgg tgatgtcatc ctctgcggaa cccagcacca ggggctcggc gccctgcagg
     1921 atggcgacca cgccgagatg accatcgaac acgtcgggtc ccttgcggta gatgtccggg
     1981 accctctcgg ccgcgagtgg ccgcgaggca tcgatgagga gatggctgcg cgccagcgcg
     2041 ccgtcggcca gtcataggga gggtagcgat cgcgcagagc ctggcccata tcgtcgcgta
     2101 ttgccggaca cagctggaac gattcacggt aatgagcagg aggcaacgat gacccagcgc
     2161 aagatgacct cgacaccccc ccgcattcag ggcagccgca tgagccgccg ccgcgccgtt
     2221 caggcgggca tggccggtgc cgccgtgttg gcggtggccg gacaggtcgg tggcgccagc
     2281 gcccgcggtg tcgggaccgc gcgcctgatg cgccaggatg gcgagccgca gcggggcggg
     2341 cggatggtga tcgcctcgac cgggcagcca gccgggctgg atgtgcagct cttcaacaac
     2401 cgcaccgtta ccctgattgc ctggaacatg cacgaggcgc tcttcacttt cgatggcgat
     2461 tacgccaccg tgccaatgct cgcggagggc atcgaggtca gcgaggacgg cctcaccaat
     2521 accattaccc tgcggcaggg agtgcccttc cacaatggtg aggagatggt ggcaaacgat
     2581 gtggtcgcct cgttcaaccg ctggatgccg atcagctcgt tggggcaagc cattcagccg
     2641 ttcctcgagt ccatcacagc ggtggacgac tacaccgtgg agatcgtgct gaccaccccg
     2701 ttggtcgcgc tgccgagcct gctcgcgcgc caaagccagg gtctcgccat catgccggct
     2761 tccatcatgg agaatgccgg cggtgagccg ctcgcgaccg aggactacat cggcaccggg
     2821 ccgtaccggt tcgtcgagtt ccaggctgac cgctacaccc tgatggagcg gttcgaagat
     2881 tacgccagcc tcgacaccga gcacagcggc tacgccggcg cgaaacccgc ctacctcgac
     2941 gagctcgaat tcgtgccggt gcccgatgag gccgcgcgcc tcgcgggctt gcagtccggc
     3001 gaataccact acctcgagga aatcatcccc gaccagatcg aggcggtcgg tggcgacccg
     3061 aacgtcacca tcgagatcct gccgccgcgg agcttcggcg tggtcacgct gaacaacgcc
     3121 ggcggtctca ccacggacgt cgccatccgc cgcgcgatcc aggctgccgt gtcggtcgaa
     3181 ccgctcgcgc aggcgtcgca tggtgagggc tacttcgagc cgggcccggg catcatgctg
     3241 ccgcagacgg catgggtctc cgaggtcggc tccgagctct acaatcaggg cgacccggag
     3301 aagtgccagc agctgctgga cgaggccgga tacgacggca cgccgatccg gatcatcacc
     3361 acgcaggagg acctcggcga ctacaacgcc gccgtggtgc tccagcagca gctcgaggag
     3421 ggtggcttca ccgtcgagct cgaggtcttc gacgaggcta cgctcgacga caacctggag
     3481 gaagaggaag gctgggagat caccaccgac tcgatcgtct tccggccgga tccggcgctc
     3541 attaatgcct acgcgagctg cacggcggac gcccagtggt gctctgacga gaaagtcgcg
     3601 atcatggagc ggatccagac cgaacaggat ccggaggccc ggttcgccgc gttcgaggag
     3661 ctgcagcagc tctggtatga cgaggttccg atggtcaaga tcaccgacca gttcggtgtg
     3721 gccgcactcg cctcaaacgt gcagggcctg ctgggcacca tgcatttcga gatcgagccg
     3781 gagttcgcga acgcctggct ctcggagtaa ggattcgtgc cgagggcggg gccaggtctg
     3841 ccggtatcgc ccggtcgagg atcggccggc gcgtcgctga ccgatccatt cctcgcaacc
     3901 agtgacaccg ggcccggcgc ctcgccgcgc ccggtcgatc ggtggctgct ttcggggagt
     3961 tgcgcgtgat tgcgtatata atccgacgcc tgttgttgct gataccggtg ctaatcatcg
     4021 tcgcggtcat cgtgttcagc ctggtgcacc tgacaccggg tgaccctacc tcggcgatgc
     4081 ttggtcgcga ggcgtccgcc gaggatcgcg ccgccctgcg tgaggagctg gggctcaaca
     4141 agccgctgct caagcagttc gttgactggt cttggggtgc gatgcggctc gacttcggcg
     4201 agtcgttctt catccgcaag ccggtcaccg aggcgttgct agatcgcgcc gagcccaccg
     4261 gcctcctcac gctgtactcc ctgacattcg ccgtcgcact cgccctaccc tcaggcgtga
     4321 tcgccgccat gaggccgaac tcgctgattg accggttgct gatggtgaca tcgatcagcg
     4381 gcgcggcgat cccgagcttc ttcttcggca ttctgctgat gcttttcttc gccgtgaacc
     4441 tcggctggct gccctcgggc ggctatcagg acattggcaa cggtttctgg cagcacttcc
     4501 gctacatgat catgccgtcc gtcgcgctcg gcttctcgtc agcgggcctg ctcgcgcgca
     4561 tcgtgcgctc gacgatgctc gatgtgctca atcaggagta catccgcacc gcctacgcca
     4621 aggggctggc ggaaaaccag gtaatccgcg ggcatgcgct tcgcaacgcg ctcatccctg
     4681 cgatgaccgt gaccggcatc atgctcgcca acatgctcgg cggcgcggtg gtgatcgaaa
     4741 cggtgttcaa catccccggc atggggcggc tgctggtgca aagcgtggtc cggcgtgact
     4801 tcccggtcgt gcaggccgcc gtgctggtgg tggccgtgat cgaagtgctg gtgatgctgc
     4861 tgatcgatat cctgtacgtg ttcgtcgacc cgaggatccg gtatggcagt gagtaggatg
     4921 gacctggcca gcaacgccac cgcgccgaag cgcgctcgcc ggttcacgcc ctatgcgatt
     4981 ccgtcgaact cgaagttcta ctggatgcgg ctggtggcgg agaaccccgc cgctgtcggc
     5041 agcctgatcc tgctgctgat catcgcgctc ggcgcgacct tcgccccggt catcacccag
     5101 ttcgaccccg attatgtgaa cccggccgac cggctcagcc cgccatcgag tacctactgg
     5161 ttcggaaccg atgatctggg acgcgatatc tacaccaggg tcgtctacgg tgcgcgggtc
     5221 tcgctgctgg tcgggctcgc tgtgatggtc ggcgcgacca ccatcggtgc gctgctcgga
     5281 ctgatcgcgg ggttctaccc gcggcgcatc gacacacccc tgatgcgcgt gatggacggg
     5341 ctcgaggcat tcccgagcat ccttctggcg atcgcgatca tggcggcgtt cggcgcggcg
     5401 gtccagaacg tcatcatcgc gctgtcggtg gtgtacgcgc ccaacatcgt gcggctggta
     5461 cgtggcacca cgctgatgaa cagccgtgag ctgtacgtag agtcggctcg cgcgatcggg
     5521 atgagggact ggtcgatcat gcacaagtac atcctgcgga actcactctc gccgctgatc
     5581 gtgcaggcga ctttcgtggt cgcgttcgcg atcctcgccg aggcatcgct gtcgttcctc
     5641 ggcgctggcg tcccgcagga catccccacc tggggcggga tggtgagcgc ggggcaggcg
     5701 cggctgcagc aggcgtggtg ggccgcggtg ttccccgggg tgatgctctt catcaccgtg
     5761 ctggccatca acctcatcgg cgacgcgctc cgcgatgcac tcgacccgcg ttcccgtcag
     5821 cgctgacctg ctcgattatc acagcttttt gacatcttgc cgtacttgtt gcccagccgc
     5881 atcagcataa acctccacgc ccggatggta gacaacaaat tgaaccatcg gcagtccatg
     5941 tagggtcttc agcactacga gtcctgaatc aactgcccgc accacggcgt ccctatcgcc
     6001 cttgccacca tgaatggtgt gatttccgac ctcccagaat gcctcaatcg cgtcaatcac
     6061 cgtgggcggt agtactgggt actcatgcca gagtgtttcc acagctttcc gcaaaggtaa
     6121 gtagtctcgg tcttggagga accccgcgct acctagcagg aatctcagtt ccttttcgat
     6181 ctccagagat aggcggatga ggactacttt gggatcatca gacgcttctt cgagaatagc
     6241 ctcgatgacc gcgtcgctgt tgtcgaccga ctctctagtg accgtcccat ccactcctgc
     6301 cttctcagag tcagccaccc cgacggctac agtttgaggg acggacctgc ttcgctgcac
     6361 agtttctgca gatgcgacgg cggtactggt cccatgtgcc gatccagacg cctcatatgt
     6421 ctttctcttc gacgctatat cctccgagga cgatcgcccg ccacgacagc gtccgatcat
     6481 ctgctaagga gctacttgga ttcatacact ttgctcacca tgttgacgca tcttctcgcc
     6541 ggagttctcg ctgcaggtga tgggcacgag accggctctc accggaactg accaccggtc
     6601 tccatgatga gcagttcgcc gtcgaagccg tcggcgatcg tagcggcacc gagctggcag
     6661 gatgccatga aggactggtc ttgcgtagca tccatgaact tcatgatcac ctcctcatca
     6721 cccggctgcc gtccggctcg tcgcgttcat tagctcagag cgtctcatag ggccactgta
     6781 ttccaaaagc tgagatctgt ccttttaaat ttatatagtc tctttttcgg gtataattta
     6841 tagagaggaa ctccacaata tgacagacga accacagctt ctttctatca gtgtggcagc
     6901 caagcggctc ggtatctcgc agggaacgct acgcgtttat gccgataagg ggatcatccc
     6961 cgtcatcacc ctcccgagag gacaccggcg attcaagccg gaagacgtcg aacgactccg
     7021 gcgggaatgg ggcattgacg aaacagatcc agggaaggac gaaagagcat gaccacgcga
     7081 cgcgggaata atgaggggtc cattacgcaa aggaaggatg ggcgctggga agggaaggtc
     7141 tcgctgggtt tccgaccgga cgggacgcgt atccgtaagt ccgtctacgg caagacgaag
     7201 acagacgtcc gaaatcagat gaaagctctt gatcgggaac atgcggcggg aatgccggtc
     7261 gacgtgaaac ggcagacggt cgcccagttc atgaatacgt ggctccagga cgtcgctaag
     7321 gggtcggtgc gaccccgaac ctacaatagt tatgcacaac ttattcgcga ccacttgaac
     7381 ccgtcgctgg gacggcatca gctatcgaag ctgacgcccc agcatgtcca gacgatgatg
     7441 aatgacaagc tagcatcagg gctgtccccc gaaccgttca atacatgcgg gcggtattga
     7501 gaagggcgct gggtcaagcg ttgaagtggg gtctggtctc gcgcaacgtc gcgacgctgg
     7561 tcgacccgcc aaagccggag catcacgaca ttcagttcct cactccggaa taggctaagg
     7621 tgttccttaa ggcagtcaag ggcgacaggc tagaagccct gtattctgtc gacatgacgc
     7681 taggtcttcg acagagcgaa gcactcggtc tgaagtggga cgatatcgac ttcgtgaaca
     7741 ggacgctagc gatacgacgg tcgctccagc gaatcaatcg ggaactgatc
//
LOCUS       DASYWL010000354         2572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_131185, whole genome shotgun sequence.
ACCESSION   DASYWL010000354 DASYWL010000000
VERSION     DASYWL010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131185"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VGR22_12475"
     CDS             complement(<1..725)
                     /locus_tag="VGR22_12475"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEV2129425.1"
                     /translation="MVRKNELKRKLRAGEKVIGVFMPPPSPLLVEVSAAAGFDFVLLD
                     NEHGPIGPETAYHMILAAEATGITPLIRVGLRDKQEVLKFLDLGIQGIMSPQVNTAED
                     AEYAVASSRYIPRGIRGLAGGRAFNFGVGIAPSDAAQQINEEMLSLIQFEHIDALPEL
                     EKIVQVPDLDVLFVGPNDLAQSMGKPGLPGDPEVTKIADEAVATAKSAGVKTGTTAFS
                     VEAAKAALDRGFDMVVANAPGLYL"
     gene            complement(833..1483)
                     /locus_tag="VGR22_12480"
     CDS             complement(833..1483)
                     /locus_tag="VGR22_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129426.1"
                     /translation="MPVGVDRPHAAAQHGKRATADLQRGVVSHTVDTERQPRHDRHAG
                     AGEVGGDAARHVLAVGSRALGTDDRNRAAVGEANIAAQEDHRRWRRYLAKQVGVGVMA
                     QRQDVDGIPAQQLEFTLRIAAIPGDGKLFQRASIQESAQLLLVGVPGFAHGAERGQQP
                     LEAHWTDPVHIAERGPVRRVLIRLVGDRSDFTRLGHHARPFLPNALRRVTWRGIYR"
     gene            1485..2168
                     /locus_tag="VGR22_12485"
     CDS             1485..2168
                     /locus_tag="VGR22_12485"
                     /inference="COORDINATES: protein motif:HMM:NF036806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEV2129427.1"
                     /translation="MSSPHASIGQGAILSDFHPDAAPDGPNFPARNRLISGLSLGVAV
                     VEAPERSGALITVDFTADQGRDVFAVAGPVAWPSSAGCNRMLRDGARLARSADDILED
                     LRLGEAPKQLPMESPAALDEPSRRVLAVLTGEPRHTDEISASAGIPIASLASTLMTLE
                     LQGLFVTSANTPRLPRLSPEKPRNSRKNRGIEVLQSLPILSVCAKTDAKTLSELALGV
                     SPDGCRGSP"
     gene            2321..>2572
                     /locus_tag="VGR22_12490"
     CDS             2321..>2572
                     /locus_tag="VGR22_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129428.1"
                     /translation="MEIVPSYRPAAVQPMEMLSCLECGDPIRYALNPHVSIRFDTSPP
                     SLKYGDRKVDSTYRAFVGDFCDRCALQYASDADRPSMLRD"
BASE COUNT          437 a          823 c          843 g          469 t
ORIGIN      
        1 aggtagaggc ccggcgcgtt ggcgaccacc atgtcgaacc cgcgatccag agccgccttc
       61 gccgcctcga cgctgaatgc cgtggtcccg gttttcacgc cggcgctctt cgcggtcgcc
      121 accgcctcgt cggcaatctt cgtgacttcg ggatcacccg gaaggcccgg cttgcccatt
      181 gactgcgcga gatcgttggg gccgacgaag agcacgtcca ggtcgggcac ctgcacgatc
      241 ttctcgagtt ccggcagcgc gtcgatgtgc tcgaactgga tgagggagag catttcctcg
      301 ttgatctgct gggcagcgtc gctcggcgcg atgccgactc cgaagttgaa ggcgcggccg
      361 cccgcgagcc cgcggatacc gcgagggatg tatcgcgagg aggcgaccgc gtactcggcg
      421 tcttccgcgg tgttgacctg tggcgacatg attccctgga taccgagatc gagaaacttg
      481 agaacttcct gcttgtcccg caggccgacg cgaatgagcg gcgtgatgcc ggtggcctcg
      541 gcggcgagga tcatgtggta ggcggtctcc gggccgatgg gaccgtgctc gttgtcgagc
      601 aggacgaaat caaaacctgc cgcggccgaa acctcgacga gcagcggcga cggtggtggc
      661 atgaacacgc cgatgacctt ctcacccgcc cgcaacttcc gcttcagttc gttcttccgg
      721 accacgcttg cactccttgt ctcgttggcg actctgccgg ccggcgcgac gttcgcgtcg
      781 ctaccgggga gtcacaccgg tcccattccg tggcatgatg acgtcattgt gatcatctgt
      841 aaatgccacg ccaggtgacg cggcggaggg cgttggggag aaaaggccgg gcatggtgtc
      901 cgagacgggt aaagtcgctc ctgtcgccga cgagccggat caggacgcgc cgtactggac
      961 cgcgttccgc aatgtgaacg ggatcggtcc agtgcgcttc gagcggctgc tggccgcgtt
     1021 cggccccatg cgcgaagcct ggcacgccga caagcaggag ttgcgcggac tcctggatcg
     1081 acgctcgctg gaacagcttg ccgtcgcccg gaatcgcggc gatccggagg gtgaattcga
     1141 gctgctgcgc cgggatgcca tcgacgtcct gacgctgcgc catgacacct accccgacct
     1201 gcttcgcgag atacctgcgc caccgccggt gatcttcctg cgcggcgata ttcgcctctc
     1261 cgaccgccgc gcggttgcga tcgtcggtac ccaacgcgcg actgcctacg gcaaggacat
     1321 ggcgcgcagc atcaccaccg acctcgcccg cgccggcgtg acggtcgtgt cggggctggc
     1381 gctcggtatc gacggtgtgg ctcaccacgc cgcgctggag gtcggcggtc gcacgcttgc
     1441 cgtgctgggc agcggcgtgc ggacgatcta cccctaccgg catcgtgagc tcgccgcacg
     1501 catcaatagg acagggggcg attctctccg acttccatcc cgacgccgcc cccgatggtc
     1561 ccaactttcc ggctcggaat cgactcatct ccggtctgtc gctgggcgtg gcggtcgtcg
     1621 aggcgccaga gcgcagcggg gcgctgatca cggtcgattt cactgccgat caggggcggg
     1681 atgtcttcgc ggtcgcgggc ccagtcgcct ggccatcgag cgcgggctgc aatcgtatgc
     1741 tgcgagatgg cgcgcgtctg gcgcgaagcg ccgacgacat cctggaggat ctgcggctcg
     1801 gtgaggcacc gaagcagctg ccgatggaat caccggcggc gctcgacgag ccatcccgcc
     1861 gggtgctcgc ggtgctgacc ggcgagcctc gacacaccga cgagatctcg gcgtcggccg
     1921 gcattccgat cgcgagcctg gcgtcgacgc tcatgacgct ggagctgcaa ggcttgttcg
     1981 taacgtcggc aaatacgccg cggctcccgc gtctgtcccc tgaaaaacct cgtaacagtc
     2041 gaaagaatag gggtatcgag gttctccaga gcttgccgat attatcagtg tgcgccaaaa
     2101 ctgacgccaa aacattatcg gaactggctc tcggtgtctc cccggatggg tgccgtggaa
     2161 gcccctgagt aaggtgctaa agatgccata gatgacaaag gtgctaacgc aacctcgcct
     2221 agcatcttag cttggcacgt cgctgatttc gggacgaaaa catcccgcgc cgggagcgag
     2281 ccggccggga tgatggtgcc cactgggaag gagtggatac atggagattg tacccagtta
     2341 caggcctgcg gcagtgcaac cgatggagat gctgtcctgc ctagaatgcg gcgacccgat
     2401 tcggtatgcg ctcaacccgc acgtcagcat ccgttttgac acgagtcctc cgagtctcaa
     2461 gtacggagat cgcaaggtcg actcgaccta ccgtgcgttc gtgggggact tctgtgatcg
     2521 gtgcgctctc cagtatgcct ccgacgccga tcggcccagc atgctgcgtg ac
//
LOCUS       DASYWL010000355        11723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_393918, whole genome shotgun sequence.
ACCESSION   DASYWL010000355 DASYWL010000000
VERSION     DASYWL010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 11723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11723
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393918"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..342)
                     /locus_tag="VGR22_12495"
     CDS             complement(88..342)
                     /locus_tag="VGR22_12495"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV2129429.1"
                     /translation="MDILWWILIGLVAGGVASLIVPGRTPGGVIGAIVVGILGGLLGG
                     WLVEDVLDLGVGGGIIGAILVAIVGAVIILFAFRQISQRT"
     gene            756..1919
                     /locus_tag="VGR22_12500"
     CDS             756..1919
                     /locus_tag="VGR22_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2129430.1"
                     /translation="MATQPQQRTSAVVDGAAPQGQPLLEVKNLKTQFFTQDGVVKAVD
                     DVSFFVMPGETLGVVGESGSGKSMTGLSLMRLIPNPPGKIVNGEVVFNGNDVLKMSED
                     QVRSIRGNDIAMIFQDPMTSLNPVLTINRQISESLQLHMGMNKNQARNRAIELLNMVG
                     IPNAQERVDQYPHQFSGGMRQRVMIAMALSCDPKLLIADEPTTALDVTIQAQILDLMR
                     NLQHETGAGVLLITHSMGVVAGMADRVQVMYAGHIVETAKTEEIFANPRMPYTVGLMQ
                     SIPRLDSQRREKLRPIRGLPPDLIDLPDMCPFTPRCNYAREKCEQKNPPLLEVKPGHY
                     AACWFWEEVSKADNPDDVARRHGHLPPSDPQAELEAEQLATKDHLDRTDIGQT"
     gene            1977..3074
                     /locus_tag="VGR22_12505"
     CDS             1977..3074
                     /locus_tag="VGR22_12505"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEV2129431.1"
                     /translation="MSAAVTEERQMEPSAAYESDGEALLDVRNLVMHFPLTQGIIFQR
                     KVGAVQAVDGVSFSVKRGETLGLVGESGCGKSTTGRAILQLYKPTSGEVHFNGRDLTK
                     IDGTEMRKMRRHLQMIFQDPYASLNPRMTVGNIVSEPMQIHKLVPKRERTERVQELLQ
                     TVGLNPYFANRYPHEFSGGQRQRIGIARALAANPDFIVADEPVSALDVSIQAQIVNLM
                     EDLQDQFGLTYLFIAHDLSVVRHISDRVAVVYLGKIVELADRNALYDDPLHPYTKALL
                     SAVPIPDPAIEKRRERIILSGDVPSPINPPSGCHFHTRCPYMMPDICPKVDPVFADQG
                     DGHYVACHLYPGSVPGDMAPGGKATTADVRR"
     gene            3075..3743
                     /locus_tag="VGR22_12510"
     CDS             3075..3743
                     /locus_tag="VGR22_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEV2129432.1"
                     /translation="MGFSIRPVEDILAILLAFVIATSIHEFMHARSAFQLGDSTARDQ
                     GRITLNPLAHFEPVGFFGMMMIAIGFPFIGWGKPVPVAPWRMSRISRERRKQGMALVA
                     FAGPLSNVVQAAIAAIVIQVVVRNGIDLGPALNVLEWFVIINALLASFNLIPIPPLDG
                     FNILNGLLPAFWEPILSPLARYGFPVLLLLIFVGGQIGGSIFASMAYPFRNTLIDLFF
                     HGLA"
     gene            3868..4254
                     /locus_tag="VGR22_12515"
     CDS             3868..4254
                     /locus_tag="VGR22_12515"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV2129433.1"
                     /translation="MMWHRLSPYDQHHLVAVAEDLESAGCDTLTVLAGLLHDIGKAGS
                     VALPARVAAVLLKPLDGVAERVRGMTDPPVGLKGLQLLLTHAERGARLLEEHGADPRV
                     VRLVRHHEDAHPDPAIAALQAADERH"
     gene            4281..5072
                     /locus_tag="VGR22_12520"
     CDS             4281..5072
                     /locus_tag="VGR22_12520"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEV2129434.1"
                     /translation="MSLNAPNGQGMLTNYQLQLPGYEGPLDVLLRLIERNQLEITDIS
                     LVAVTSQFLAHVGELNEAPAEVTASFATVGTRLAVLKSRSLLPKPTSVVEDDAPSDLT
                     QQLLEYRRARDASAFLARRAEQNLRSYARPLPVPVEPALRHTSERLIDYPASALVGAL
                     RRRLTVLPKSGRMIPARRVVSLRETLNRLLELTGIRQRVPFSQIVSPYVSRTEVATAF
                     LGMLVLMRRAVISAEQSELFCEIHLNRNTDTEDAGEIYERFESTA"
     gene            5047..5736
                     /gene="scpB"
                     /locus_tag="VGR22_12525"
     CDS             5047..5736
                     /gene="scpB"
                     /locus_tag="VGR22_12525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEV2129435.1"
                     /translation="MSDSRAQPDAAEDRVVGDLTQLVIPGAEPGHRLSDAYIGAIIEA
                     MLFVAPEPPTVSELAAGAELDPEEIERGLEVLRRGPTRGLVVQRHGETVTLGTAPALA
                     DHVRRFLRLDREARLSSAAMETLAIVAYRQPVTRSEIEAVRGVDCSGVIATLHARNLI
                     ESVSRRLTIGNPIQYGTTPEFLRHFGLSSLADLPSLGSINGEPGQDVLGAAMQEPPPP
                     SDRQPDQVSPG"
     gene            5733..6701
                     /locus_tag="VGR22_12530"
     CDS             5733..6701
                     /locus_tag="VGR22_12530"
                     /inference="COORDINATES: protein motif:HMM:NF016533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEV2129436.1"
                     /translation="MTTVAVRGHGELTLPPVLLTNLRAVHGDRFEPWLVGLGPMLAGA
                     LGELDARIVPTDLDLSYHLVLFARRAGGEDIVVKCTVPNAEQPPEVAGVEALSAAGIG
                     PRLLWKDLERGVFAMERIVPGERLPTHIPTLEEDAKIIATIGDIAARMSRKTDITRYA
                     GRLVTVRDYTRALDTYDPHSQLWQNHRDEIARARALRDAMLSPPDRRDTFVHGDLHHY
                     NLLVDAMGEPKVIDPKGLVGPAGYDFGVMSYNPMGIQRHPDLAALFRERVTVWSEAVG
                     LPWGEVRDWGYVGAMVSACWESEGGAQGWQDALLSAVTIRELTQPS"
     gene            complement(6685..7884)
                     /locus_tag="VGR22_12535"
     CDS             complement(6685..7884)
                     /locus_tag="VGR22_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEV2129437.1"
                     /translation="MTYGREGVYLDHAATTPTDPAVVESMLPWFTERFGNPSSIYQIG
                     QEARAAIDNARRQIAGVLRCVPSEVLFTSGATESNNLAIRASIGAWRQKHGWDRKPHV
                     VISAIEHHAVLDVVLQLRDEGLAVDIVPCDSIGRVDPAAVEAAVKPETCLISVMLVNN
                     EIGTVQPVPEIAAVARERGIVVHTDAVQAAGLRPIAAQHIGVDMLSLSAHKFYGPKGV
                     GVLFVRKGTPMRFQQRGGSQEAGRRGGTENVPLIVGMGEALQRADAISDQYATHCRVL
                     RDRLWKQLQERVPGILRNGTDDPESAQPNILNVVLPGVQGETMLLNLDLLGVSASAGS
                     ACTTGNPEPSHVVRAIGRNDEECRASLRFSVGRGNSLSEMDEAARVVAESVERVRALS
                     GTVSSAG"
     gene            complement(7931..8389)
                     /locus_tag="VGR22_12540"
     CDS             complement(7931..8389)
                     /locus_tag="VGR22_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEV2129438.1"
                     /translation="MKVSTRGEYGVRAMVALARHYGEGPLSIADIARESSVPPAYLEQ
                     LIAPLRRASLVTSKRGARGGYTLSREPEAVRIGDIYRVMEGPVAPMDCVSENEADQTC
                     PLIDGCETRPVWLKVRDSIVEALDSTTLADLANKRGAQLPGTSTEVKTIA"
     gene            complement(8473..9627)
                     /locus_tag="VGR22_12545"
     CDS             complement(8473..9627)
                     /locus_tag="VGR22_12545"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEV2129439.1"
                     /translation="MRAFRVARLLDVDIHIHPTFLLIVAWAVARWGFGSDGGVGSLLL
                     GLVFVLLIMVSVVVHELGHVLMAREFGVRVLDVTLWPFGGVARIEQIPAHSRAEFMIS
                     LAGPAMNFAIFVALLPVLILVGLIGGRDALLAGAELFDRITLVGLLTYVALTNLFIMV
                     FNLIPAFPMDGGRVLRAALVNIVGRDRATTVAVTLGSLLALLAAVWGVWDRSLVLVAV
                     GIFVAIAAQAEARAAHAEEGMRRLRVGQFSLWDMGGISPDQPLTLAMRGGPRDLVVTK
                     NGEVLGMLWRADLLRSLQGGMGGRTVADVMDRSVYVADVNDSVLDVQRVMNSLNRWAV
                     PVTENGQYRGIFTAERFVHLHRQVMPPVFSGWSLPEPWRDAIRHNLRVRR"
     gene            9883..11385
                     /locus_tag="VGR22_12550"
     CDS             9883..11385
                     /locus_tag="VGR22_12550"
                     /inference="COORDINATES: protein motif:HMM:NF016827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129440.1"
                     /translation="MEAGFELIPEMRQWVSPSLIEANYILSLSQQELQAVIEQELLSN
                     PALELEERSTCPACGSSLEGSWCPTCRAEPAPSLDRVAGTDDENEWRVSSVASEVDED
                     FDPLSIVASRADPREAILSDARILLEPREHPIAGYLIDSLDARGFLNEDVATLASEIG
                     APCNEVVEVLEVIQDVAPVGVGARSLAECLHLQADFLRRSGEEVPEHVSYLIDNHLDD
                     LGAHKFGKLARQLGVTPDDIEAARDFIRDHLNPHPMQLTQARSWGSPTDSPLVSPDVM
                     VHIDNDDLIVEIVDSRYNYLRMDGVYSDLLARISRQPKDKKAEPAPEAETDDEDEVEG
                     FEEETVAEAATSMKSEERDHIRQYSNRARLFISNIEQRRETLLKISKVVCEFQEEFLR
                     GGVRNLKPLTRAVVAQQVGVHESTVSRATANKYVMLPNNKVIPYSDFFRPSLSTKDVI
                     KEIIENEESKGCSITDREICDRLLDEGIRIARRTVAKYRSELGILPSTMR"
     gene            complement(11509..>11723)
                     /locus_tag="VGR22_12555"
     CDS             complement(11509..>11723)
                     /locus_tag="VGR22_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2129441.1"
                     /translation="FILTGPDARARGGPEVWRMERDPYLLETSNPGVFAAGEVRLGSA
                     KRVATAVGEGASAVLSVWQYRAAMGL"
BASE COUNT         2155 a         4023 c         3614 g         1931 t
ORIGIN      
        1 gctgagaaca gcataccacc cacaccgccc cccggagacg cgtctcgggg ggcggtgtgg
       61 gtcaatacgg cccggtcacc acagggatca ggtccgctgg ctgatctgcc gaaaggcgaa
      121 caggatgatc accgcgccga cgatcgcaac cagaatggcg ccgatgatgc cgccgcccac
      181 gccgagatcg agcacatcct cgacaagcca accaccgaga aggccaccga ggataccgac
      241 cacgatcgcg ccgatgacgc cacccggcgt gcgaccggga acgatcaggc tggcaacacc
      301 gccagccacg agacctatca ggatccacca cagaatgtcc atcgtctcat ctcctcctgt
      361 ttggtctgac acccatcgcc ggaaccgtag cgatggctgc taccatgcac gccagaccgt
      421 ccgtacgtcc actctgttgc aagtcgagtg ccagcaggac tcgcatccct gcccaattgg
      481 ccatacagct tgacgccgga cattgcggtc ccgaaactga cgtggtatcg tgcactaaca
      541 gtaaatcctc gccaaaacag gggatgggca gcgcaacgga gcgcggaatc gaaccgattg
      601 gtcacgggcc agccggcctt tccggctttg cagtgaccgc cggatttcga tcttcgggat
      661 ttcgcacggt ctggcgctgg ctgtatcggg tgtctggcaa ggattggccc cggcgcagag
      721 tcagcagtga aacaccaacg gcgagaggag tcaggatggc aacacagcca cagcagcgca
      781 cgtcggccgt cgtggacggg gccgcccccc agggccagcc cctgctcgag gtcaagaacc
      841 tgaagaccca attcttcacg caggacggtg tcgtcaaggc cgtcgacgac gtgtcgttct
      901 tcgtgatgcc aggcgagacg ctcggcgtgg tgggcgagtc cggttccggc aagagcatga
      961 cgggactctc cctcatgcgg ctgattccga atccgcccgg caaaatcgtc aatggtgagg
     1021 tcgtcttcaa cggcaacgac gtgttgaaga tgagcgaaga tcaggtccgg tcgatccgtg
     1081 gcaacgacat cgccatgatc tttcaggacc cgatgacctc gctgaacccc gtgctcacga
     1141 tcaaccggca gatcagcgag tcgctgcagc tgcacatggg catgaacaag aaccaggccc
     1201 gcaaccgcgc gatcgagctg ctcaatatgg tcggtatccc caacgctcag gagcgcgtcg
     1261 accagtaccc gcaccagttc tcgggcggta tgcgccagcg cgtgatgatc gccatggcgc
     1321 tctcgtgcga cccgaagctg ctgatcgccg acgagcctac caccgcgctg gacgtgacga
     1381 tccaggcgca gatcctcgac ctgatgcgca acctgcagca cgagacgggt gccggcgtgc
     1441 tgctgatcac ccactcgatg ggtgtggtcg ccggcatggc cgaccgcgta caggtcatgt
     1501 acgccggcca catcgtggag acagcgaaga ccgaagagat tttcgccaac ccgcggatgc
     1561 cctacacggt cgggctgatg cagtcgatcc cccggctcga ctctcagcga cgcgagaagc
     1621 tccgtccgat ccgcgggctt ccgcctgatc tgatcgacct gccggacatg tgtccgttca
     1681 ccccgcgctg caactacgcc cgggagaagt gtgagcagaa gaatccgccg ctcctggagg
     1741 tcaagccggg ccactacgct gcgtgctggt tctgggaaga ggtctcaaag gccgacaacc
     1801 cggatgatgt cgcgcgccgg cacggtcacc tgcccccatc ggatccccaa gcggagcttg
     1861 aggctgagca gcttgccacc aaggaccacc tcgaccgcac agacatcgga caaacctgat
     1921 cgcccgaagc gtggcgcaac agatctcgat gatccgaatc tcggaggaac atggatatgt
     1981 cagcggctgt cactgaagaa cgccaaatgg agccgtcagc tgcctacgaa tccgacggcg
     2041 aggctttgct cgacgtccgc aacctggtca tgcacttccc gctgacccag ggcatcatct
     2101 tccagcggaa ggtcggtgcc gtgcaggccg tcgacggcgt ttcgttcagc gtcaagcgcg
     2161 gcgagacgct cggtctcgtg ggcgagtccg gctgcggcaa gtccaccacg ggacgggcca
     2221 tcctgcagct ctacaagccg acctcgggcg aggtccactt caacgggcgc gacctgacca
     2281 agatcgatgg caccgaaatg cgcaagatgc gccgccacct gcagatgatc ttccaggacc
     2341 cgtacgcctc cctgaacccg cgcatgacgg tcggcaacat cgtgtccgag ccgatgcaga
     2401 tccacaagct ggtgccgaag cgggagcgca ccgagcgcgt gcaggagctg ctgcagacag
     2461 tcggtctcaa cccgtacttc gccaaccgct acccccacga gttctcgggc ggacagcggc
     2521 agcgtatcgg catcgcgcgt gcgctggcgg cgaacccgga cttcatcgtc gccgacgagc
     2581 ccgtttcggc gctcgacgtc tccattcagg cgcaaatcgt caacctgatg gaggatctgc
     2641 aggatcagtt cggcctgacc tacctcttca tcgcgcacga cctctccgtc gttcgccaca
     2701 tctccgaccg cgtcgccgtg gtgtacctcg gcaagatcgt cgaactcgcc gaccgcaatg
     2761 cgctctacga tgatccgctg cacccgtaca ccaaggctct gctctcggcg gtgccgattc
     2821 ccgaccccgc cattgagaag cgccgcgagc gcatcatcct ctcgggcgac gtgccctcgc
     2881 cgatcaatcc gccctcgggc tgccacttcc acacccgctg cccgtacatg atgcctgaca
     2941 tctgcccgaa ggtggacccg gtgttcgcgg accagggcga tggtcactat gtggcgtgcc
     3001 acctctaccc aggctcggtc cccggtgaca tggcgccggg tggcaaggca acgacggcag
     3061 acgtgcggcg gtagatgggc ttttcgatcc ggccggtcga ggacatcctt gcgatccttc
     3121 tcgcgtttgt catcgccacg tcgatccacg agttcatgca cgccaggtcc gcgttccagc
     3181 tgggcgacag caccgctcgc gaccaaggcc gcatcaccct gaacccactg gctcactttg
     3241 agccggtggg tttcttcggg atgatgatga ttgccatcgg cttccccttc atcggctggg
     3301 gcaagccggt ccctgtggcg ccgtggcgca tgagccgcat ctctcgggag cgacgaaagc
     3361 agggcatggc gctcgtcgcc tttgccggac cgctctccaa cgtggtgcag gcagccatcg
     3421 ccgcgattgt gatccaggtc gtggtccgca acggtatcga tctgggtccg gcgctcaacg
     3481 tcctcgagtg gttcgtcatc atcaacgcgc tgctcgcctc gttcaacctg atcccaatcc
     3541 cgccactgga cggcttcaat atcctgaacg ggctgctgcc cgcgttctgg gagccgatcc
     3601 tctcgcctct ggcccgctat ggcttcccgg tcctcctgct gctgatcttc gtcggtggcc
     3661 agatcggcgg ctcgatcttc gcgagcatgg cctatccatt ccgcaacacc ctgattgatc
     3721 tcttcttcca cggactcgcc tgaacctgcc ctgaccatgc caccgtaccg tgcccgcctc
     3781 cgccagggtc tcaccgccct cttccgctcg cgcccgccgt tggagtttcc ggcacggtgg
     3841 tcagcccatg ctccggatga ggtccggatg atgtggcacc gtctctcccc ctacgaccag
     3901 caccacctcg tcgcggtcgc ggaagacctc gaatcagcgg gctgcgatac tctcacggtc
     3961 ctggctggcc tgctgcacga catcggcaag gctggctccg tcgcgctccc cgctcgggtc
     4021 gctgctgtcc tcctcaaacc gctggacggc gtggccgaac gcgtacgcgg gatgaccgat
     4081 cccccggtcg ggctcaaggg actacaattg ctgctgaccc acgccgagcg cggtgcccgc
     4141 ctgttggaag agcacggcgc agacccgcgc gtggtccgac tcgtccggca ccacgaggat
     4201 gcccatcccg acccggcgat cgcggccctg caggctgccg atgagcgcca ctgagaggcg
     4261 tacacctgag gaaggtcgag atgagcctca acgcgcccaa cgggcaaggc atgctcacga
     4321 actaccagct ccagctcccc ggctatgagg gccctctcga tgtccttctg cgcctcatcg
     4381 aacgcaacca gctggagatc accgatatct cgctggtggc agtgacctcg cagttcctgg
     4441 cccacgtcgg cgagctgaac gaggcgccgg cagaagtgac cgcgtcgttc gcaactgtcg
     4501 gcacgcggct cgccgtgctc aagagccggt ccctgctgcc gaagccaacg agcgtcgtgg
     4561 aggacgacgc gccctcggac ctgacccagc agctcctgga gtaccgccgc gcgcgtgatg
     4621 ccagcgcgtt tctcgcaagg cgcgccgagc agaacctgcg ctcctacgcg cgcccccttc
     4681 ccgtgccggt tgaaccggca cttcggcaca catccgagcg cctgatcgac tacccggcgt
     4741 ccgctctcgt cggggcgctg cgacgccggc tcaccgtcct gccgaagtcg ggcaggatga
     4801 ttccggctcg cagggtcgtc agcctgcgcg agacgctcaa ccgtctgctg gagctgaccg
     4861 gaatccgcca gcgcgtgccc ttctcccaga tcgtgagccc gtatgtctcg cggaccgagg
     4921 tggcaacggc ctttctcggc atgctggtgc tcatgcgccg cgccgtcatc tccgccgagc
     4981 agtccgagtt gttctgcgag atccacctga accgaaacac tgacaccgag gacgccggag
     5041 agatctatga gcgattcgag agcacagcct gacgccgccg aggaccgagt cgtcggcgac
     5101 ctgacccagc tggtgattcc gggagccgag ccaggccatc gcctgtccga cgcctacatc
     5161 ggcgcgatca tcgaggcgat gctctttgtc gctccggagc cgccgaccgt gagtgagctg
     5221 gcggcgggcg ctgagttgga ccccgaggaa atcgagcgcg gtctcgaggt gctgcgccgt
     5281 ggcccgacac gtggactggt ggtccaacgg catggcgaga ccgtcacgct cggtaccgca
     5341 ccggcgctgg ccgaccatgt ccggcgattc ctgagattag accgggaggc gcggctctcc
     5401 tcggcggcga tggagacgct ggcgattgtt gcctaccgtc agccggtcac tcgcagcgaa
     5461 atcgaggcag ttcgcggcgt cgactgttcc ggcgtgatcg caaccctgca cgcacgcaac
     5521 ctgatcgaat cggtttcccg gcgactaacc atcggcaacc cgatccagta cgggaccacg
     5581 ccggagttcc tgcgccactt cggactgagt tcactcgcgg acctgccgag tctcggtagc
     5641 atcaacggcg agcccggcca ggacgttctt ggggccgcga tgcaggaacc gcctccacca
     5701 tccgatcgac aaccggatca ggtcagtcca ggatgaccac cgtcgctgtt cgagggcacg
     5761 gtgagttgac cctgccgccc gtgctgctga cgaatctccg ggccgtccac ggcgatcgct
     5821 tcgagccgtg gctcgtcggg ctgggcccaa tgctcgccgg cgcgctcggc gagctcgacg
     5881 ctcgcatcgt gccgacggac ctcgatctct cctaccacct tgtcctcttc gctcggcggg
     5941 ccggcggcga ggacatcgtc gtcaagtgca cggtgccgaa cgcggagcag ccgccagagg
     6001 tggcgggtgt cgaagccctc tcagccgccg gcatcgggcc gcgtctgctg tggaaggacc
     6061 tcgagcgcgg cgtgttcgcc atggagcgca ttgtgccggg tgagcgactg ccgacgcaca
     6121 tcccgacgct ggaggaagac gcgaagatta ttgccaccat cggcgacatc gccgctcgca
     6181 tgagtcgcaa gactgacatc acccggtacg cgggccggct ggtgacggta cgggactaca
     6241 cgagggcgct cgacacgtac gacccacact cccaactctg gcagaaccac cgggacgaga
     6301 tcgctcgggc gcgtgcgctg cgcgacgcca tgttgtctcc tccggatcgc cgggacacgt
     6361 tcgtccacgg cgacctgcac cactacaacc tcctggtcga tgccatggga gagccgaagg
     6421 tgatcgaccc gaaggggctg gtgggcccgg ccgggtatga cttcggcgtg atgagctaca
     6481 acccgatggg catccagcgc cacccggacc ttgccgcgct ctttcgcgag cgcgtgacgg
     6541 tctggtccga agccgtgggc ctgccgtggg gtgaagtgcg cgactggggc tacgtcggcg
     6601 cgatggtcag cgcttgctgg gagtcggagg gcggcgcgca aggctggcag gacgcgctgc
     6661 tgagcgcagt gacgattcgg gagctcaccc agccgagctg acggtcccgc tcagcgctcg
     6721 cacgcgctcg acgctttccg caacgaccct cgccgcctca tccatctctg acagcgagtt
     6781 cccacgccca acagagaacc ggaggctcgc gcgacactcc tcgtcgttcc gtccgatggc
     6841 gcggacgaca tggctcggct ccggattccc ggtcgtacac gcggatcccg ccgaggctga
     6901 cacgccgagg agatcgaggt tgagcagcat cgtctccccc tggaccccgg gaagtaccac
     6961 attgagaatg ttgggctgtg cggactcagg gtcgtccgtc ccgttgcgca ggatgccggg
     7021 cacccgctcc tgcagctgtt tccagaggcg gtcgcgcagt acgcggcagt gcgtggcgta
     7081 ctggtcgcta atggcatccg cgcgctggag cgcctcgccc atgccgacga tcagcggcac
     7141 gttttcggtg ccacctcgcc ggccagcttc ctggctacct ccgcgctgct gaaagcgcat
     7201 gggtgtcccc ttgcgtacga agaggacgcc aacccccttc ggcccgtaaa acttgtgggc
     7261 cgagagcgag agcatgtcca ccccgatatg ctgggcagca atcggtcgga gtcccgccgc
     7321 ctgcaccgcg tccgtgtgca ccacgatccc gcgctcgcgc gcgacggcag cgatctccgg
     7381 caccggctgc accgtgccga tctcgttgtt gacgagcatg acgctgatca ggcacgtctc
     7441 cggcttgacc gccgcctcga cggcggcagg atcgaccctg ccgatgctgt cgcatggaac
     7501 gatgtccacc gcgagcccct cgtcgcggag ctgcaggaca acatccagca cggcgtggtg
     7561 ttcgatcgcg gagatgacca cgtgtggctt cctgtcccac ccgtgctttt gccgccaggc
     7621 gccgattgac gcgcggatgg cgagattgtt gctctcagtg gctccactgg tgaagagcac
     7681 ctcgctcggc acgcaccgga gcaccccggc aatctgccgc cgagcgttgt cgattgctgc
     7741 ccgagcctcc tgtccgatct ggtagatgct gctggggtta ccgaagcgct ccgtgaacca
     7801 cggcagcatc gactcaacca ccgcggggtc cgtcggggtc gtcgccgcat ggtccaggta
     7861 gacgccttcc cggccatagg tcatggcgtg atcgtctcca tccggaaggt gttggcgtgc
     7921 agaatgacga tcaggcgatc gtctttacct cggtagacgt accgggcagt tgggcgccgc
     7981 gcttgttggc aagatccgcc agggtcgtcg agtcgagggc ctcgacgatg gaatcgcgga
     8041 ccttgagcca gaccgggcgc gtctcgcacc cgtcgatcag cggacaggtc tgatccgcct
     8101 cattctcgga gacgcaatcc atcggcgcca ccgggccttc catcacccgg tagatgtcac
     8161 caatgcgcac cgcctccggc tcgcgagaga gggtgtagcc accgcgcgcg ccgcgcttgc
     8221 tcgtcacgag gctggcacgc cgcaacggtg cgatgagttg ttccaggtag gcaggaggaa
     8281 cagatgactc ccgggcgatg tcagcgatcg acagcgggcc ttcaccgtag tgccgggcca
     8341 atgcgaccat cgccctgaca ccatattctc cccgtgtcga caccttcatg atgcagagcc
     8401 tcctgtcaga agtggcatca cttcaatttg gatactgtac ggtgcgcggg aaagaaggtc
     8461 aaccgaagcg ggttaccgac gcacgcgcag gttatgccga atggcatccc tccacggctc
     8521 cggaagggac cagccggaga agacgggcgg catcacctgc cggtgtaggt gcacgaagcg
     8581 ttccgccgtg aagatgccac gatactgacc gttttcggtc accggcacgg cccagcggtt
     8641 caacgagttc atcacccgct gcacatcaag cacagagtcg ttgacatcgg cgacgtagac
     8701 ggagcggtcc atcacatcgg cgacggttcg gccgcccatg ccgccctgca ggctgcgcag
     8761 caaatccgct cgccagagca tgcccagcac ctcgccgttc ttcgtcacca cgagatcgcg
     8821 cggtccaccc cgcatcgcaa gcgtcaacgg ctgatccggt gagattccgc ccatgtccca
     8881 gagggagaac tgcccgaccc tcaggcggcg catgccctcc tccgcgtggg cggcgcgagc
     8941 ctctgcctgg gcggcgatcg ccacgaagat tcccaccgca accagcacga gcgagcgatc
     9001 ccagacgccc cacacggcgg ccaacagcgc caacaggctc cccaaggtga cagcgacagt
     9061 cgtcgcccgg tcacgaccga cgatattgac gagggcggcg cgcagcacgc gcccgccgtc
     9121 catcgggaac gccgggatga ggttgaacac catgatgaag aggttggtga gcgcgacgta
     9181 ggtcaacaaa ccgaccagcg tgatgcggtc gaacaactcg gcaccggcca gcaaggcgtc
     9241 gcgcccccca atgaggccca ctaggatcaa aacgggcaga agggcgacga agatcgcgaa
     9301 gttcatggcg ggaccagcga gcgagatcat gaactctgcg cgcgagtgcg ccggaatctg
     9361 ctcgatgcgc gcgacgccgc cgaagggcca aagcgtaaca tcaagcacgc gcacgccgaa
     9421 ttcccgcgcc atcaggacat ggcccagctc gtggacgacc acggaaacca tgatcagcag
     9481 gacaaagacg aggcccagca acagcgagcc aacacctccg tccgatccga agccccagcg
     9541 cgccaccgcc cacgcgacga tcagcagaaa cgtgggatgg atgtggatat cgacatcaag
     9601 cagtcgcgct acgcggaacg cgcgcattgc gagcacccct tcaggccgga cacagaggcg
     9661 accagggcgc ccgtgatcgt ggtgttccag cgcggcggaa ctgtgcgctt cttagcaaga
     9721 tcgttgccat cacttctccc aagccctata ctttcgtgat agaggaacta accatcgcag
     9781 gtggtggaag ccccagcgtt gttgcccggt cgcggatatg tcgaccacaa gtatcgcatt
     9841 tctgccgttc ccgcacagca atgcgagcga ttgaggtgat agatggaagc cggatttgaa
     9901 ctcataccag aaatgcgaca gtgggtatcg cccagcctga tcgaagccaa ctacatcctc
     9961 agcctgtctc agcaggaact ccaggcggtg atcgagcagg aactgctctc caaccctgct
    10021 ctcgagctgg aagagcgctc tacctgtccg gcctgcggga gctcactgga gggatcatgg
    10081 tgccccacct gtcgggcaga acccgccccg tcacttgacc gggtcgccgg cacggacgac
    10141 gagaacgaat ggcgcgtctc cagcgtggcg tcggaggtgg atgaagattt cgacccactc
    10201 tcgatcgtcg cctctcgcgc cgatccaaga gaagcaatcc tctccgacgc ccgtattctc
    10261 ttggagcccc gagagcatcc catcgccggg tacctcattg actccttgga cgcgcgcggc
    10321 ttcctcaacg aggacgtggc tacgctcgcc agcgagatcg gcgcgccgtg caacgaggtc
    10381 gtggaggttc tggaggtcat ccaggatgtt gccccggtcg gcgtcggcgc ccggtcgctc
    10441 gccgagtgcc tgcacctcca ggccgatttc cttcgccgct cgggcgagga ggtccccgag
    10501 cacgtcagct atctcatcga caaccatctc gacgacctcg gggcgcacaa gttcggcaag
    10561 ctcgcgcgcc aactcggcgt cacgccggac gacatcgaag ccgcgcgcga cttcatcagg
    10621 gaccacctca atccgcaccc gatgcagctg acgcaggcgc gctcctgggg ctccccgacc
    10681 gacagccccc tagtgtcgcc ggacgtcatg gtgcacatcg acaacgacga cctcatcgtc
    10741 gagatcgtcg acagccggta caactacctg cgaatggacg gcgtctacag cgatctgctc
    10801 gcccgcatct ctcgccagcc gaaggacaag aaagccgagc cggcacccga ggccgagacg
    10861 gacgatgagg atgaggtgga agggttcgag gaggagaccg tcgccgaggc cgcaacctcg
    10921 atgaaatccg aggagcggga tcacatccgg cagtacagca accgggcgcg cctcttcatt
    10981 tcgaacattg agcagcgccg cgagaccctg ctcaagatca gcaaggtcgt ctgcgagttc
    11041 caggaggagt ttctccgcgg cggcgtgcgg aacctcaagc cgctcacgcg cgcggtggtc
    11101 gcacagcagg tcggtgtgca cgagtcgacg gtgagccgtg caacggccaa caagtacgtc
    11161 atgctgccga acaacaaggt catcccatac agcgacttct tccgcccgtc cctgagcacg
    11221 aaggacgtga tcaaggagat catcgagaac gaggagtcga agggctgcag catcaccgac
    11281 cgggagatct gcgaccggct gctggacgaa ggcatccgca tcgcccgccg caccgtcgcc
    11341 aagtaccgct ccgaactcgg aatcctgccc tcgaccatgc gctagcgtcg cagcattgcc
    11401 ctccccggtc cctgctttgc gctgaaccgg ggctgtccgc ctcgttggaa cccgggacga
    11461 tcgccaggat cgcctccgct acgcccccat ctgtgcgccc ggcggccgtc acagacccat
    11521 cgcggcgcgg tactgccaga cggagaggac ggccgacgca ccctcgccca cggcagtcgc
    11581 gacccgcttc gccgagccga gtcgcacctc acctgcggcg aatacccccg ggttgctcgt
    11641 ctccaacagg taggggtcgc gctccatgcg ccacacctcc ggcccaccgc gtgcccgggc
    11701 gtcgggcccg gtcaggatga agc
//
LOCUS       DASYWL010000356         9562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6280
            k141_562850, whole genome shotgun sequence.
ACCESSION   DASYWL010000356 DASYWL010000000
VERSION     DASYWL010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955732
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:23:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,520
            CDSs (total)                      :: 2,483
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9562
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562850"
                     /isolate="SMAG_U6280"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VGR22_12560"
     rRNA            <1..412
                     /locus_tag="VGR22_12560"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            501..619
                     /gene="rrf"
                     /locus_tag="VGR22_12565"
     rRNA            501..619
                     /gene="rrf"
                     /locus_tag="VGR22_12565"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            640..714
                     /locus_tag="VGR22_12570"
     tRNA            640..714
                     /locus_tag="VGR22_12570"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:672..674,aa:Met,seq:cat)
     gene            complement(788..3022)
                     /locus_tag="VGR22_12575"
     CDS             complement(788..3022)
                     /locus_tag="VGR22_12575"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEV2129442.1"
                     /translation="MYDPLAGRFEPTLSERTVFTEDDAPKLLYGMDSTARVVAIEPYG
                     SNEVLLYKRDGRGRTVSERRVTSPWVLSSDLRPFRGTGDADIAELRGEHSYRYLITFP
                     SWDAYRTATTGETLPRGTVIGPPTITQQYQLRSGLTLFKGMEYADLRRMQIDLETLTL
                     GPAAPDAGVIMISVRQGNREEILVRDSTEEDLFERFTRLVTKLDPDVIEGHNLYRFDF
                     PYLVHRATKVGVPLLLGRNRSLPRVVDVNGRQASVYIHGRHVIDTYQQIQRFDVSGSL
                     SRYGLKSVIHEMGLEREDRTFVEGASIADMWRQGHRELDQLAAYALDDVRDVDALSRV
                     IMPTMFYQTQMLPTTYQRSTLTGTGRKIDDFMFRTYLAAGHSIPRPTPGRTYAGGYAE
                     VVHSGIFEPIVKCDVESLYPSIMLRESIASSTDVLQAFPVLLRDLTRRRIDAKRKAAT
                     SEGEARATWTALQASFKILINSFYGYLGFGGGAFNDYTAAEHVSLEGQRLIREVVQHL
                     RDLGATPIEIDTDGAYFSPPDSVSTEADEHAFIARISDRLPTGINLAHDGRYWRMLSL
                     KIKTYALLDHSGGITLKGSALRSRALEQCFQEFIRYAAERFLHHDRDAVRERYFNLAE
                     AIQRRALPVEAISQWTTIRHDSMNSRRRLKRLLDEHPGQWHDGERVEVYERADGSLAF
                     AEHYADDENVAVLLRRLHEVAERFRPLIGDDAGFDAMFPRITPITNLAAARDRKPARQ
                     LTLM"
     gene            3232..4434
                     /locus_tag="VGR22_12580"
     CDS             3232..4434
                     /locus_tag="VGR22_12580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEV2129443.1"
                     /translation="MANNTGAQGGARSAMAAGALLGAAALVWAGKRVQEFATTDRPEG
                     LVNWERALEIALRMNRESALTATQREELSASYAQLVDTCIPLISDYTQTTLPQSARET
                     FAFDRVDWVHANLAGFQRMFAPIEDLDAGPGKNRNAASRLAGGVNQAVLSSEIGVLLG
                     YMAKRVLGQYDLALLGREPVTAGKLYFVEPNIRGLERKLDLPKDDFRMWLALHETTHA
                     FEFEAYPWVREHFNGLLERYMEFMRQDAEYLRQGVEGMKVMLQRLREGRSGNGEEAGS
                     WIESFMNGEQRALFTEMQALMSVIEGYSNHVMNAVGRDLLPTYDLISKRFEERQKHRS
                     TGEKLFAQLTGLNVKMEQYRQGEAFVNAIVERHGPEAIERLWAGPESIPSMSEVRNPD
                     EWSARIFA"
     gene            complement(4512..6047)
                     /gene="purH"
                     /locus_tag="VGR22_12585"
     CDS             complement(4512..6047)
                     /gene="purH"
                     /locus_tag="VGR22_12585"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEV2129444.1"
                     /translation="MRALISVSDKSGIEPFARALHERRMEIVSTGGTARALESVGIPV
                     TPVSEVTHFLEMLDGRVKTLHPNIHAGLLAKLEDPAHRSQLEQAGVEPITVLVVNLYP
                     FERTATSPAASQDGIVESIDIGGPAMVRAAAKNFAHVYVVTSPDDYDAVLEALDTPDT
                     EHLALRKHLAAKAFGHVSTYDSLVAAWLRSDDEPFPEELTLGLRRSIVPKYGENAHQE
                     AAVYASLRPGQPAPGLLDAEQIKGETLSFNNFLDADAAWNAAQLFQEPTVAIVKHTVP
                     CGLAVRQTLVEAYRAAYEGDSVSAFGGIVALNRAVDAATAQQMRKLKLDIIIAPGFAD
                     DSLDLLRKKKATRILQMPNRQGARIHELDVRTIAGAMLVQEPDITEESVDDWQVVTKR
                     APTAEERRDLEFAWRVIPSIRSNAILFAKDRAIVGLGAGQPNRLESVTIAARKAGPKA
                     KGAVMASDAFFPFPDGVEQGIDAGITAVVQPGGSVRDEEVIAAADAAGISMLFTGTRH
                     FRH"
     gene            complement(6175..7635)
                     /locus_tag="VGR22_12590"
     CDS             complement(6175..7635)
                     /locus_tag="VGR22_12590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEV2129445.1"
                     /translation="MSRWQPNQKIVVAVVYVSAMLLNSLDATMINVALPTLAREFDVS
                     PASTESVVLGYLVSLAVFIPVSGWVGDRFGTKRTFLAALFIFTVGSLASGFATSLNTL
                     VAMRVLQGVGGGMLTPVGMAMLYRTFAPHERIRVGRVLMFALILGPALGPIIGGAILG
                     AWSWPWIFFIKVPLSTAALVFGLLFLHEHREEEPGRFDAAGFLLAGVGFASIMYGLSQ
                     GPQHGWESAAVAILVGGLVAMALFVVVELRQSKPMIDLRLLGNRLFRATLLVSFFASA
                     AFLGVLFLVPIFLQEVVRLSPLETGISTAPEAIGVVVSTQLVARIYPHVGPRRLMTAG
                     LTIVTGAILLMSEFRPESNIWFIRGTMFLLGTGMAFIFLPNQAASLATITRRDTGRAT
                     TLTNVQRQLGSALGVALLSTVLSTVGAVAVAQNGVMIPNVDAYRAAFFVAAGIAVIGA
                     LWATRVPDEEAAETMVTGSERARRSGTDNAASAAKP"
     gene            7844..8689
                     /locus_tag="VGR22_12595"
     CDS             7844..8689
                     /locus_tag="VGR22_12595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEV2129446.1"
                     /translation="MATVDVLIPTYGRKTGLAMVLTSLLGQTFTDFDVVVSDQTDEAE
                     SYLDSIEIQTAVQALRYHGHQVHLHRHLPRRGLAEQRQFLLDQSNAPYVHFLDDDVVV
                     EPNVMERLLSVIRSDQCGFAGSPAAGLWFLEDERPHQQGIEIWEGPVRPEGITPDTIP
                     EYRHGVNVAANALHVERRLAPAGQTIRYKVAWIGGANVLYDREKLLDVGGFGFWDRLP
                     EEHAGEDAVVQFLLIHAYGGCGVLPTGTYHLCLDTTVPNRETNAAELFGDLLEEWRSR
                     HGCDS"
     gene            8903..>9562
                     /locus_tag="VGR22_12600"
     CDS             8903..>9562
                     /locus_tag="VGR22_12600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEV2129447.1"
                     /translation="MVSFGIKTAPQHTTYDAMLRVWQEAEAIPSIEHAWLFDHFAPIQ
                     GDLDGPCLEGWTVLAAFAAVTERLRLGLMVTGNTYRHPAVLAKIAATVDVISEGRLDF
                     GIGAGWNEYEHESMGIPLYQPGERIRRLDEACEMIKRLFTEPTVDFDGRYYQLKEARS
                     EPKPVQRPYPPLVIGGGGEQLTLRVVAKHADVWNYTGGSVEEFRHKVGVLRRHCEEVG
                     RN"
BASE COUNT         1760 a         2905 c         3171 g         1726 t
ORIGIN      
        1 ttggcacctc gatgtcggct cgtcgcatcc tggggccgga gaaggtccca agggttgggc
       61 tgttcgccca ttaaagcggt acgcgagctg ggttcagaac gtcgtgagac agttcggtct
      121 ctatccgctg tgggcgtagg atgtgtgagg ggggccgtcc ctagtacgag aggaccggga
      181 tggacgaacc gctggtgagc cagttgtcgc gaccgcggca tcgctgggta gccacgttcg
      241 gcatggcgaa ccgctgaagg catctaagcg ggaatccggc cccgagacga gacatcccag
      301 gggcataagc cccgagagac tcccggcaga cgaccgggta gataggcgcg aggtggacag
      361 cgggcaaccg actggagctg acgcgtccta atagtcgatg cggagttggg tcaaccgaca
      421 gcaggcatgg tcttcgtggt gagcgcggag gggccggtgg tccctcgctt gcaacagtgg
      481 tgtgtgcgac gatgcggggt gcccgtggcc gagagcggat cggccccacc cggacccatc
      541 ccgaacccgg tcgtgacccg gtccagcgcc ggagagtact gtggcggcga cgccacggga
      601 ggcgaggccg ctgcgggcac cccgcatcgc cgcacccgac gcggggtgga gcagtggcag
      661 ctcgttgggc tcataaccca aaggtcggag gttcgaatcc tccccccgct accaagatcg
      721 agaaaatgga aaccccggag gcactgcctc tggggctttg ctgtattcct gctttctccg
      781 cagccttcta cattagggtg agttggcgcg caggcttgcg gtcgcgggcc gcagcgagat
      841 tcgtgatcgg cgtgatacgg ggaaacatgg cgtcgaaccc tgcgtcgtcg ccgatcagcg
      901 gtcggaaccg ctccgcgact tcgtgcagcc ggcggagcag cacggcgacg ttctcgtcgt
      961 cggcatagtg ttcggcgaac gcgagtgatc cgtcggcccg ctcgtacact tcaacgcgct
     1021 ctccatcgtg ccactggccg ggatgttcgt ccagaaggcg cttcaggcgc ctgcgactgt
     1081 tcatcgaatc gtgccggatc gtggtccact ggctgatcgc ctcgactggc agggcgcgcc
     1141 tttgtatcgc ttccgcgagg ttgaagtagc gctcgcggac ggcatcgcga tcgtggtgga
     1201 ggaagcgctc agcggcgtac cgaatgaact cctggaagca ttgctccagg gcgcggctgc
     1261 gaagcgcgct gcccttgagc gtgatgccgc cgctgtggtc gaggagtgcg tacgtcttga
     1321 tcttgagtga cagcatgcgc cagtagcggc cgtcgtgggc cagattgatg cccgtgggca
     1381 acctgtcgct gatgcgcgcg atgaaggcgt gttcatccgc ctcggtgctg acgctatctg
     1441 gaggcgagaa gtaggcgccg tcggtatcga tctcgatggg cgttgcaccg agatcgcgaa
     1501 gatgctggac gacttcgcgg atgagccgct gtccctcaag cgagacatgc tctgctgccg
     1561 tgtagtcgtt gaaggcgccg ccgccgaatc ccaggtagcc atagaacgaa ttgatcagga
     1621 tcttgaagct cgcctgcagt gccgtccagg tcgctcgtgc ttcgccttcg gaggtcgctg
     1681 ccttccgctt ggcgtcgatc cggcgtcgcg tgaggtcgcg gagcaacacg gggaatgcct
     1741 gaagcacgtc cgtcgacgat gcgatcgatt cacggagcat gatggatgga tagaggctct
     1801 cgacgtcaca tttcacgatc ggctcgaaga tgccagaatg gacgacctcc gcgtagccac
     1861 cggcgtacgt ccgacccggc gttgggcgcg gaatgctgtg gccggccgcc aggtaggtcc
     1921 gaaacatgaa atcgtcgatc ttgcggcctg tgcctgtcag cgtggaacgc tggtatgtcg
     1981 tgggcagcat ctgggtctgg tagaacatcg ttggcatgat gacacgggag agggcatcga
     2041 catcgcgaac atcatccagt gcgtaggccg caagttgatc gagttcccgg tggccttggc
     2101 gccacatgtc ggcgatcgac gcgccttcga cgaaggtgcg gtcctcccgc tccaggccca
     2161 tctcgtggat tacgctcttc aatccgtatc gcgataggga gcccgacaca tcaaaccgct
     2221 gaatttgctg gtacgtgtcg atgacatgtc gaccgtggat gtagaccgac gcctgtcgcc
     2281 cgttaacatc gacgacgcgt ggcagggacc tgttgcgtcc cagaagcagc ggtacgccaa
     2341 ctttcgtggc ccggtgcacc agatagggga agtcgaatcg gtagaggttg tgaccctcga
     2401 tcacatccgg gtcgagcttg gtgacgagcc gtgtgaatcg ttcgaacagg tcttcctcgg
     2461 tcgagtcacg gaccagaatc tcttcacggt ttccctgacg aacagagatc atgatgacgc
     2521 cggcatcggg agcggcaggg ccgagcgtca gcgtctcgag atcgatctgc atgcgccgga
     2581 gatcggcgta ctccatccct ttgaacaggg tgagcccact gcggagctgg tactgctgag
     2641 tgatcgttgg cggccctatg acggtacccc gaggcagagt ctcgcctgtg gtcgccgttc
     2701 ggtaggcatc ccacgaaggg aaggtgatca ggtaccggta ggaatgttcg ccacgcagtt
     2761 cggcaatatc ggcatccccg gttccgcgga atgggcgaag atcactgctc aggacccaag
     2821 ggctggtcac tcggcgttcg cttaccgtgc gaccgcggcc gtcccgcttg tagagcagga
     2881 cttcgttgga cccgtacggc tcgatcgcaa cgacgcgcgc cgtcgaatcc atcccgtaaa
     2941 gcagcttcgg tgcatcatct tccgtgaaaa ctgtccgctc cgagagtgtc ggttcaaatc
     3001 ggccggcgag cggatcgtac atgcgattgc tttccaggcg cgctcaggcg caggttctgg
     3061 gccagtctcg tcaagtctac tcctttggcc gtcaacgaaa tcctgcgcgc acacgcctcg
     3121 gcggtccgcc gcccgaacta cctgccacgg gcgcctggtg taggcttacg cgtatcgcag
     3181 gtctcttctg gggcgcaacg tgcgacggag tcgctgcatt aaaggtggag tatggctaac
     3241 aatacaggcg cccagggcgg tgcacgatcg gcgatggcag caggtgcgct gctcggcgcc
     3301 gccgcgctcg tttgggccgg gaagcgtgtg caggagttcg ccacgaccga tcgaccggag
     3361 ggactggtca actgggagcg agcccttgag atcgcgctcc gcatgaacag ggagagtgcg
     3421 ctcaccgcta cccagcgcga ggagctctcg gcgagctatg cccaactcgt cgacacgtgc
     3481 atcccattga tatcagacta cactcaaacg acccttccgc agagcgcgcg cgaaacgttc
     3541 gcgttcgacc gggtcgactg ggtccacgcc aacctcgccg ggttccagcg catgttcgcg
     3601 ccaatcgagg atctggacgc gggtcccggc aagaaccgca acgctgcctc ccgattggcg
     3661 ggcggtgtaa accaggctgt gctcagcagt gagatcgggg tcctgctcgg ctacatggcc
     3721 aagcgcgtgc tcggacagta cgatctcgcg ctgctcggcc gcgagccggt aacagccgga
     3781 aagctctact tcgtggagcc gaacatccgg ggcctggagc gcaaacttga tctgccgaag
     3841 gacgacttcc gcatgtggct cgcgctccac gagacgacgc acgcgttcga gttcgaggcg
     3901 tacccctggg tgcgggagca cttcaacggc ttgctggagc gctacatgga attcatgcgc
     3961 caggacgctg agtatcttcg ccaaggcgtc gaaggcatga aggtcatgct ccagcggctc
     4021 cgcgagggac gaagcggcaa cggcgaggaa gcgggcagct ggatcgagtc cttcatgaac
     4081 ggcgagcagc gagcgctttt caccgagatg caggcgttga tgagcgtcat cgagggctac
     4141 tcgaaccatg tgatgaacgc tgtcggccgt gatctgcttc cgacctacga tctgatctcc
     4201 aagcggttcg aggaacggca gaagcatcgc tcgaccggcg agaaactctt cgcccagctc
     4261 actggcctca acgtgaagat ggagcagtac cggcagggcg aggcgttcgt gaacgcgatc
     4321 gtcgagcgcc atggccccga ggcgatcgag cgtctttggg ctggacccga atcgatccca
     4381 tcgatgtccg aggtgcgcaa cccggacgag tggtcagccc gcattttcgc ctagcgggcc
     4441 gccgggcagg aagagagcaa aagccgcgcc gtcttccgcc gcgtgccgtc gtgtttgcgt
     4501 gagccggccg gctaatgccg gaagtggcgc gtgccggtga acagcatcga aatcccggct
     4561 gcgtcggcgg ccgcgatcac ctcctcgtct cttacggacc ctccgggctg caccactgca
     4621 gttatgccgg cgtctatgcc ttgctccaca ccgtccggga agggaaagaa ggcatcgctc
     4681 gccatcactg cacccttcgc ctttggccct gccttgcggg cagcgatggt gacgctttcg
     4741 agacggttcg gctggcccgc ccctaggccg acgatcgccc tgtccttcgc gaagagaata
     4801 gcgttggacc tgatcgacgg aatcacgcgc caagcgaact ccaggtcgcg tcgctcctcg
     4861 gcagtcggcg ctctcttcgt aaccacttgc cagtcgtcaa ccgactcctc ggtgatgtcc
     4921 ggctcctgca ccagcatcgc gccagcgatg gtccgtacgt ccagctcatg gatgcgagca
     4981 ccttggcggt taggcatttg caggatgcgt gtcgccttct tcttgcgtag caagtccagc
     5041 gagtcgtccg caaagccggg agcgatgatg atatcaagct tcagcttgcg catctgctga
     5101 gcggtggcgg cgtccaccgc tcggttcagc gcaacgatgc cgccgaaggc cgacacgctg
     5161 tcgccctcgt aggccgcgcg gtatgcctca acgagcgtct gccgaaccgc aagcccgcaa
     5221 ggcaccgtgt gcttgacgat agcaaccgtc ggttcctgga agagctgtgc cgcgttccag
     5281 gcggcgtcgg cgtccaggaa gttgttgaac gagagggttt cgcccttgat ctgctcagcg
     5341 tcgagcaatc ctggcgccgg ctggccgggt cgtagactgg cgtagacagc agcttcctgg
     5401 tgggcgtttt caccgtactt tggcacgatc gatcgccgga gcccgagggt cagctcctcg
     5461 gggaacggct catcatcaga ccggagccac gccgcgacga ggctgtcgta ggtggagacg
     5521 tgcccgaacg ccttcgctgc cagatgcttg cgcagcgcaa ggtgctccgt gtccggcgtg
     5581 tcgagcgcct ccagcaccgc gtcatagtca tcgggtgagg tgacgacgta gacatgcgca
     5641 aagttctttg cggcggcgcg gaccattgcc ggcccgccga tgtcgatact ctcgacgatg
     5701 ccgtcctgag acgcagcggg tgacgtcgcc gtccgctcga atgggtagag gttgacgacc
     5761 agcaccgtaa ttggctcgac gcctgcctgc tcgagctgcg agcggtgggc gggatcctcg
     5821 agcttcgcca gcaggccggc gtggatgttt ggatggaggg ttttgaccct cccgtcgagc
     5881 atttccagaa agtgagtcac ttcgctcact ggcgtcaccg ggattcccac cgactccaga
     5941 gcgcgcgccg tgccgccggt ggagacgatc tccattctgc gttcgtgcaa cgcgcgcgca
     6001 aacggttcga tacccgactt gtcggaaacg ctgatcagcg ctcgcatggt gttcgttgct
     6061 gctccattcg ttgtcattcg tttagagcgt ggaggcggac gtgccaccct ggctggaacg
     6121 gtagcacgag acggtccttg ggtcgcgtgc gagccgccga gcgacctgca agagtcaggg
     6181 cttggctgca gacgccgcgt tatcggtccc tgagcgccga gcgcgctcgc tgccagtgac
     6241 catcgtttct gcggcttcct catccggcac gcgagtcgcc cataatgcac cgatgacggc
     6301 gatgccggcg gccacgaaga aggcggctcg ataggcgtcg acgttcggga tcatcacgcc
     6361 attctgcgcc acggcaaccg cgccaacggt ggagagcacg gtgctgagca gcgccacgcc
     6421 gagtgcggag ccgagctggc gctggacatt ggtgagggtg gtggcacggc cggtatcgcg
     6481 gcgggttatc gtcgccaggc tcgccgcctg gttgggcagg aagatgaacg ccatccccgt
     6541 cccgagcagg aacatggttc ccctgatgaa ccagatgttg gactccggcc gaaactccga
     6601 catgagaagg atagcgcccg tcacgatcgt cagtcccgcc gtcattaacc ggcgtggtcc
     6661 gacgtgtggg tagatgcggg cgaccaactg ggtcgacacc accacgccga tcgcctcagg
     6721 tgcggtcgaa ataccggttt ccagcgggct gagacgcacg acttcctgca ggaagatcgg
     6781 aaccagaaag agcacgccga ggaaggccgc tgacgcgaag aaggagacga gcagcgtggc
     6841 ccgaaacagc cggttgccga gcaaccgcag gtcgatcatc ggcttggact ggcgcaactc
     6901 gacgacgacg aagagcgcca tggcaaccag cccgcctacc agaatcgcga ctgctgccga
     6961 ttcccaaccg tgttgcggtc cctggctcag gccgtacatg atcgacgcga aaccgacgcc
     7021 ggcgagcagg aatcccgcgg catcgaacct tcccggctct tcctcgcgat gctcgtggag
     7081 gaagagaaga ccgaaaacca gggccgccgt gctcagcgga accttgatga agaaaatcca
     7141 gggccaggac cacgcaccga ggatcgcccc gccgatgatc ggaccgagtg cggggccgag
     7201 gatgagcgcg aacatgagca cgcggccaac ccggatgcgc tcgtgcggtg cgaaggtgcg
     7261 gtagagcatc gccattccga ccggcgtcag catgccccca ccgactccct gcaagacacg
     7321 catcgcgacg agcgtattga gcgacgttgc gaaaccgctg gccagcgagc caacggtgaa
     7381 aatgaagagc gcggcgagga acgtccgctt ggtgccgaag cgatcgccga cccagccgga
     7441 gaccgggatg aagactgcga gactgacgag gtagcccaga acgaccgatt cggtcgatgc
     7501 ggggctgacg tcgaactcgc gagcgagcgt ggggagcgcg acattgatca tcgtcgcgtc
     7561 gagggagttg agcagcatcg cgctcacgta tactacggcc acaacgattt tctggttcgg
     7621 ttgccagcgg gacatcaagg aataacctgt tctgtggtgc gcgtgcaggc ggttggaggg
     7681 gcagcagcgt cccgtcaggc attctagccg cgtttttcgg agcgtgaaag gcgatccgtg
     7741 gatgtgcgcg ctactcctcc tgattgcggt cggtacgcat cttactcaac ctggattgct
     7801 tcgcctgcaa ttagccgcag tctgaaggga gtctggatac gcagtggcaa cggttgatgt
     7861 gttgatcccg acgtatggac gcaagaccgg gctcgccatg gtcttaacga gtctgcttgg
     7921 tcagacgttc accgatttcg acgtcgttgt gtccgatcag accgacgagg cggagagcta
     7981 cctcgacagc atcgagatcc agacggcggt acaagcgctt cgctaccacg gccatcaggt
     8041 gcatctgcac cgtcacctgc cgaggcgagg tctcgcggag cagcgccagt ttctgcttga
     8101 tcagagcaac gcaccgtacg tgcactttct cgacgacgac gtggtggtcg agccgaacgt
     8161 catggagcga ctgttgtccg tcattcgatc cgaccaatgc ggatttgcgg ggtcaccagc
     8221 agcaggcctt tggtttctcg aggacgagcg gccccaccag caaggcatcg agatctggga
     8281 gggaccggtt cgcccggagg gcattacgcc ggatacaatt cccgagtatc gtcatggagt
     8341 caacgttgcg gcgaacgcgc tacacgttga gcgaaggctg gcgccggccg ggcaaaccat
     8401 acgctacaag gtagcgtgga tcggcggcgc caacgtgctc tacgaccgcg agaagctcct
     8461 tgacgtgggc gggttcggct tctgggaccg actgccggaa gagcacgccg gcgaagacgc
     8521 cgtggtgcag tttctgctta tccacgcata cggtggctgt ggagtcctgc cgactggcac
     8581 ctaccacctg tgccttgata ctacggtgcc caatcgagaa acgaacgcag cagagctgtt
     8641 cggtgatctt ctggaggagt ggagatcccg tcacggctgt gacagctaga cccccgagtc
     8701 accggcggga aatccgcgct gccgactcag gcacttccag gctcgtcccg gcatccttga
     8761 tggggcgccc cagcagatcg gcttggaagc ggccctcggg ctctcgcgag ccgctcttgt
     8821 ttttctgcta ccctgactgg agcggccata ctccggtttg cggttggcct ggaactgctt
     8881 ggcaaagaaa gaggggagag cgatggtcag ctttgggatc aagaccgcgc cgcagcacac
     8941 cacctacgac gcgatgctgc gggtctggca ggaagccgag gcaattccca gcatcgagca
     9001 cgcctggctc ttcgaccact tcgccccgat tcagggcgac ctcgacgggc cctgcctcga
     9061 gggctggacg gtgctcgccg ccttcgcggc cgtgaccgag cggctgcggc tggggttgat
     9121 ggtgacgggc aatacctacc gtcacccggc ggtgctggcc aagatcgcgg cgaccgtcga
     9181 cgtcatctcc gagggccggc ttgacttcgg catcggggcc ggctggaacg agtacgagca
     9241 cgagagcatg ggcatcccgc tctaccagcc gggcgagcgc atccgacggc tggatgaggc
     9301 gtgtgagatg atcaagcggc tgttcaccga gccgaccgtg gacttcgatg gacgctacta
     9361 ccagctcaag gaggcgcgca gcgagcccaa gccggtgcag cggccctacc cgccgctcgt
     9421 gatcgggggt ggcggcgagc agctgacgct gcgcgtggtc gccaagcatg cggatgtgtg
     9481 gaactacacc ggcgggtcgg tcgaggaatt ccgccacaag gtcggggtcc tgcgccggca
     9541 ctgcgaggag gtgggacgga ac
//
